title: Extracellular RGD-binding proteins modulate cell adhesion.
abstract: Cell/fibronectin adhesion in extracellular matrices is partly mediated by integrin receptor recognition of RGD domains in fibronectin. Since blood contains significant levels of soluble fibronectin we have now investigated the occurrence of extracellular RGD-binding proteins. Attachment assays indicate that extracellular RGD-binding proteins prevent cell adhesion, suggesting their potential as novel secreted modulators of blood-borne cell adhesive interactions. These extracellular RGD-binding proteins also showed electrophoretic changes with reducing agents, suggestive of intrachain disulphide bonds, like those found in RGD-binding integrins. However, they differed from the latter in their electrophoretic profile, which was greatly dependent on the presence of protease inhibitors. Plasma from tumor-bearing mice showed a greater proportion of fast-migrating RGD-binding species under reducing condition compared to similarly treated normal plasma, suggesting that tumor development is associated with a partial degradation of extracellular RGD-binding proteins.
authors.affiliation: I.V.I.C., Caracas, Venezuela.
authors.forename: A
authors.initials: A
authors.lastname: Ciarrocchi
authors.forename: M S
authors.initials: MS
authors.lastname: Rieber
authors.forename: M
authors.initials: M
authors.lastname: Rieber
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002846:Chromatography, Affinity; D005260:Female; D005353:Fibronectins / Q000378:metabolism*; D016023:Integrins / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000378:metabolism*
chemical_list: D005353:Fibronectins; D016023:Integrins; D009842:Oligopeptides; D011480:Protease Inhibitors; D011971:Receptors, Immunologic; C047981:arginyl-glycyl-aspartic acid