Synthetic peptide with antiproliferative activity: a short C-terminal fragment of the human interferon alpha-2 molecule.
The biological activity of six synthetic peptides of the 124-144 region of the human interferon alpha-2 (IFN alpha-2) molecule was studied. Peptides were examined for their ability to inhibit mitogen induced proliferation of human blood cells in vitro. Only the peptide corresponding to the amino acid sequence 124-138 (2438) possessed IFN-like antiproliferative activity. Other tested synthetic peptides did not affect cell proliferation in this experimental system. As with the native IFN alpha-2 molecule, the inhibitory effect of the peptide 2438 was dose-dependent. On simultaneous addition of peptide 2438, antiproliferative activity of IFN alpha-2 was enhanced. Direct cytotoxic effects of synthetic peptide 2438 were not revealed. These results suggest that a synthetic peptide corresponding to the 124-138-amino acid sequence of the human IFN alpha-2 molecule serves as a cytostatic agent.
Danilkovich
A V
AV
Department of Cellular Physiology and Immunology, Biological Faculty, Moscow State University, U.S.S.R.
Freze
K V
KV
Shevalier
A F
AF
Samukov
V V
VV
Kirkin
A F
AF
Gusev
M V
MV
D000595:Amino Acid Sequence; D004305:Dose-Response Relationship, Drug; D006801:Humans; D016898:Interferon-alpha / Q000737:chemistry* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000378:metabolism; D013329:Structure-Activity Relationship
D016898:Interferon-alpha; D010446:Peptide Fragments; D010835:Phytohemagglutinins