Nephritogenicity of the lprcg gene on the MRL background.
The novel lymphoproliferative and autoimmune lprcg gene, originally discovered in the CBA/K1Jms (CBA) strain of mice, was transferred onto the MRL/MpJ (MRL) strain background, and the resultant partially congenic MRL-lprcg/lprcg carrying 93% or more MRL genomes on average were examined for immune-complex glomerulonephritis and serological aberrations. Ordinary histological studies revealed that MRL-lprcg/lprcg mice developed glomerulonephritis histologically indistinguishable from that in MRL-lpr/lpr but at a lower frequency than in MRL-lpr/lpr mice. Glomerular immune complex deposition was almost the same in MRL-lprcg/lprcg and MRL-lpr/lpr mice. The levels of serum Ig, circulating immune complexes and autoantibodies in MRL-lprcg/lprcg were comparable to or even higher than those in MRL-lpr/lpr mice. Comparison of the serological abnormalities between MRL-lprcg/lprcg with glomerulonephritis and CBA-lprcg/lprcg without it evidenced the enhanced class switch from IgM to IgG responses in both class-specific autoantibody responses and serum Ig levels in MRL-lprcg/lprcg as in MRL-lpr/lpr mice. These results taken together indicate that the lprcg gene functions in much the same manner as lpr in induction of glomerulonephritis and serological abnormalities on the MRL background as expected from the allelism between the two mutant genes.
Kimura
M
M
Department of Infectious Diseases, University of Tokyo, Japan.
Ogata
Y
Y
Shimada
K
K
Wakabayashi
T
T
Onoda
H
H
Katagiri
T
T
Matsuzawa
A
A
D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis; D000936:Antigen-Antibody Complex; D001327:Autoimmune Diseases / Q000235:genetics* / Q000276:immunology; D003176:Complement C3 / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005921:Glomerulonephritis / Q000235:genetics* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007668:Kidney / Q000033:anatomy & histology / Q000276:immunology / Q000473:pathology; D008180:Lupus Erythematosus, Systemic / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009929:Organ Size; D011507:Proteinuria
D000974:Antibodies, Antinuclear; D000936:Antigen-Antibody Complex; D003176:Complement C3; D007074:Immunoglobulin G; D007075:Immunoglobulin M