The primary structure of peptide Y (PY) of the spiny dogfish, Squalus acanthias: immunocytochemical localisation and isolation from the pancreas.
1. Endocrine cells within islets, exocrine parenchyma and ductal epithelium in the pancreas of the spiny dogfish, Squalus acanthias, were immunostained with an antiserum to the C-terminal region of mammalian neuropeptide Y (NPY). 2. Radioimmunoassay of pancreatic extracts with the same antiserum detected immunoreactivity in the dorsal lobe (338 pmol/g) and ventral lobe (433 pmol/g). Reverse phase HPLC analysis of both extracts resolved a single immunoreactive peptide. 3. The primary structure of the isolated peptide was established as: YPPKPENPGEDAPPEELAKYYSALRHYINLITRQRY.NH2. 4. Peptide Y (PY) from Squalus acanthias is identical in primary structure to an NPY-related peptide isolated from the pancreas of Scyliorhinus canicula and has a 31/36 residue homology with porcine NPY. The 5 substitutions are highly-conservative.
Pan
J Z
JZ
Comparative Neuroendocrinology Research Group, School of Biology, Queen's University of Belfast, Northern Ireland.
Shaw
C
C
Halton
D W
DW
Thim
L
L
Johnston
C F
CF
Buchanan
K D
KD
D000595:Amino Acid Sequence; D000818:Animals; D004284:Dogfish / Q000145:classification / Q000235:genetics*; D005399:Fishes / Q000235:genetics; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010179:Pancreas / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D009479:Neuropeptides; C076795:spiny dogfish peptide Y