[Effects of fatty acids on hepatic amino acid metabolism in isolated perfused liver in rats].
Large disparity in experimental conditions concerning the use of isolated perfused liver led us to investigate the effects of two free-fatty acids on amino acid metabolism in this model. Oleate uptake was 210 +/- 46 nmol/min.g and octanoate uptake 550 +/- 60 nmol/min.g. Fatty acid utilization led to an increase in ketogenesis, enhanced by octanoate in accordance with its known availability for beta-oxidation. In our experiments neither oleate nor octanoate modified hepatic amino acid exchange and metabolism as assessed by the measurements of amino acids fluxes and glucose and urea production.
De Bandt
J P
JP
Laboratoire de Biochimie A, Hôpital Saint-Antoine, Paris.
Cynober
L
L
Rey
C
C
Coudray-Lucas
C
C
Lim
S K
SK
Poupon
R
R
Giboudeau
J
J
D000596:Amino Acids / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002210:Caprylates / Q000378:metabolism / Q000494:pharmacology*; D006003:Glycogen / Q000032:analysis; D006885:Hydroxybutyrates / Q000032:analysis; D066298:In Vitro Techniques; D007657:Ketone Bodies / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009829:Oleic Acids / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D002210:Caprylates; D006885:Hydroxybutyrates; D007657:Ketone Bodies; D009829:Oleic Acids; D006003:Glycogen
Effets des acides gras sur le métabolisme hépatique des acides aminés dans le foie de rat isolé et perfusé.