Calbindin D-28K- and parvalbumin-reacting neurons in the hypothalamic magnocellular neurosecretory nuclei of the rat.
The distribution of calbindin D-28K- and parvalbumin-reacting neurons in the hypothalamic magnocellular neurosecretory nuclei of the rat was studied using the avidin-biotin-immunoperoxidase method and highly specific monoclonal antibodies. Incubation with anticalbindin D-28K-antiserum revealed immunoreactive neurons in the following nuclei: supraoptic, paraventricular (both in the magnocellular and parvicellular regions), circularis, fornicals and medial forebrain bundle. Incubation with parvalbumin antiserum displayed immunoreactive neurons only in the circularis nucleus. Additionally, it was possible to observe scattered calbindin and parvalbumin immunoreactive neurons (which do not form part of the nuclei considered) located in the hypothalamic area between the supraoptic and the paraventricular nuclei, especially for the calbindin D-28K antiserum.
Sánchez
F
F
Department of Human Anatomy and Histology (Faculty of Medicine), University of Salamanca, Spain.
Alonso
J R
JR
Arévalo
R
R
Carretero
J
J
Vázquez
R
R
Aijón
J
J
D000818:Animals; D064026:Calbindins; D005260:Female; D007031:Hypothalamus / Q000737:chemistry* / Q000166:cytology; D007124:Immunoenzyme Techniques; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000737:chemistry*; D009490:Neurosecretory Systems / Q000737:chemistry* / Q000166:cytology; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry; D010320:Parvalbumins / Q000032:analysis*; D016548:Prosencephalon / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D013495:Supraoptic Nucleus / Q000737:chemistry; D009626:Terminology as Topic
D064026:Calbindins; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G