[Effect of intratumoral injection of the mixture of OK-432 and fibrinogen (OK/fbg) on gastric cancer].
We have reported the intratumoral injection of OK/fbg is a very effective immunotherapy for colorectal cancer. In this study, we injected OK/fbg into gastric cancer. Histopathological examinations revealed the formation of fibrin fibers, marked infiltration of inflammatory cells and regression of tumor tissue. Seven days after injection, cytotoxicity of splenic lymphocytes was significantly high and HLA-DR, CD25+ splenic lymphocytes were increased. But there was no significant change in peripheral blood and lymph node lymphocytes. On the contrary, several hours after injection, cytotoxicity and surface markers were changed in peripheral blood and lymph node lymphocytes. However, there was no change in splenic lymphocytes. We suppose that activation of splenic lymphocytes was caused by activation of peripheral blood and regional lymph nodes.
Takeda
T
T
Dept. of Surgery 2, Osaka University Medical School.
Wakasugi
T
T
Katsumoto
Y
Y
Sakita
I
I
Nagaoka
H
H
Morimoto
H
H
Tsujinaka
T
T
Monden
T
T
Shiozaki
H
H
Shimano
T
T
D017024:Chemotherapy, Adjuvant; D003602:Cytotoxicity, Immunologic; D005340:Fibrinogen / Q000008:administration & dosage*; D006801:Humans; D007167:Immunotherapy; D015552:Injections, Intralesional; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D010844:Picibanil / Q000008:administration & dosage*; D013154:Spleen / Q000276:immunology; D013274:Stomach Neoplasms / Q000276:immunology / Q000601:surgery / Q000628:therapy*
D010844:Picibanil; D005340:Fibrinogen