Nucleotide sequence of a mouse Tcp-1 pseudogene: a nucleotide record for a t complex gene carried by an ancestor of the mouse.
We have isolated clones of a processed pseudogene of mouse t complex polypeptide 1 (Tcp-1) and determined the nucleotide sequence of the pseudogene. The pseudogene was 1363 bp long and had no intron. The Tcp-1 pseudogene had 88.4% or 88.3% nucleotide identity to the mouse Tcp-1 cDNA of wild-type (Tcp-1b) or t haplotype (Tcp-1a), and 87.5% identity to the rat Tcp-1 cDNA. On 12 nucleotide positions where the open reading frames (ORFs) of mouse Tcp-1b and Tcp-1a cDNAs have bp substitutions, the Tcp-1 pseudogene had 6 bp identical to Tcp-1b, 5 bp identical to Tcp-1a and 1 bp not identical to neither. On ten amino acid positions where TCP-1B and TCP-1A polypeptides have substitutions, deduced amino acids of the Tcp-1 pseudogene had four amino acids identical to TCP-1B, five amino acids identical to TCP-1A and one amino acid identical to neither. These results suggest that the ancestral mouse Tcp-1 gene would have had no significant difference between the resemblance to Tcp-1b and that to Tcp-1a before they were diverged and that amino acids of TCP-1B and TCP-1A would have been substituted in similar high rates.
Kubota
H
H
Department of Microbial Genetics, Osaka University, Japan.
Morita
T
T
Satta
Y
Y
Nozaki
M
M
Matsushiro
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D010802:Phylogeny; D011544:Pseudogenes; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013735:Testicular Hormones / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region
D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D013735:Testicular Hormones; D004247:DNA; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases