title: Differential inhibition by acetazolamide on carbonic anhydrase distribution in the quail kidney: a proposal for a membrane-bound isoenzyme.
abstract: The effects of different concentrations of acetazolamide, a specific carbonic anhydrase inhibitor, have been investigated in the quail kidney. The histochemical patterns, interpreted by means of quantitative analyses proved that 0.1 microM acetazolamide inhibited the enzyme activity in all the reactive tubular segments except for distal tubules. At this site, the reaction product disappeared from the cytoplasm but strong positivity persisted at the apical surface. The luminal staining was still present at higher inhibitor concentrations up to 0.8 microM acetazolamide. Under histophotometric analyses, the residual reactivity proved to be nearly the same at the increasing inhibitor concentrations assayed. The validity of the results was checked by similar investigations in other control tissues. On the basis of the properties known for carbonic anhydrase in mammalian kidney, we conclude that the luminal membrane staining in the quail distal tubules might be due to a carbonic anhydrase isoenzyme that is similar, both in affinity for acetazolamide and in intracellular localization, to the membrane-bound enzyme purified from mammalian proximal convoluted tubules.
authors.affiliation: Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Gabriella
authors.forename: P
authors.initials: P
authors.lastname: Palatroni
mesh_terms: D000086:Acetazolamide / Q000494:pharmacology*; D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis*; D002417:Cattle; D006168:Guinea Pigs; D006651:Histocytochemistry; D007425:Intracellular Membranes / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D007527:Isoenzymes / Q000032:analysis*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008168:Lung / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D011784:Quail; D051381:Rats
chemical_list: D007527:Isoenzymes; D002256:Carbonic Anhydrases; D000086:Acetazolamide