[Improved measurements of anti-L-asparaginase IgG antibody and its clinical applications].
To elucidate the usefulness in prediction of hypersensitive reactions of L-asparaginase (L-asp), anti-L-asp IgG antibody was measured by two kinds of enzyme-linked immunosolvent assay (ELISA) with improved microplate method and original immunobeads method. The correlation coefficient of both methods matched very well (R = 0.949). Nine out of the 10 patients who were treated with L-asp for the first time showed the elevation of the anti L-asp IgG antibody and returned to normal range within 9 months thereafter. Twenty-two out of 26 patients (84.6%) who had ever developed hypersensitive reactions showed the positive anti-L-asp antibody. There was no positive correlation between the anti-L-asp titer and the severity of the hypersensitive reactions. We concluded that the ELISA with microplate was simple and useful methods to determined the anti-L-asp IgG antibody.
Tsukimoto
I
I
First Department of Pediatrics, Toho University School of Medicine.
Matsui
J
J
Iwashita
H
H
Shigeta
K
K
Suzuki
H
H
Hashimoto
T
T
D001215:Asparaginase / Q000276:immunology*; D002648:Child; D004342:Drug Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D011237:Predictive Value of Tests
D007074:Immunoglobulin G; D001215:Asparaginase