Differential expression of cytochrome P-450 in proliferating and quiescent cultures of murine lung epithelial cells.
Expression of the cytochrome P-450 monooxygenase activity 7-ethoxyresorufin O-deethylase (7-ERD) was surveyed in proliferating and quiescent cultures of murine cell line C-10, a non-tumorigenic line of presumed alveolar type II origin. 7-ERD activities were undetectable in subconfluent/proliferating cultures but became detectable once the cultures had become confluent and their growth had arrested due to contact inhibition. Serum deprivation of subconfluent cultures resulted in a rapid inhibition of cell proliferation and the subsequent expression of 7-ERD. These results suggest that 7-ERD expression is regulated as a function of the proliferative status of C-10 cells.
Reiners
J J
JJ
Research Division, University of Texas M.D. Anderson Cancer Center, Smithville 78957.
Pavone
A
A
Cantu
A R
AR
Auerbach
C
C
Malkinson
A M
AM
D000818:Animals; D002455:Cell Division / Q000502:physiology; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010078:Oxazines / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics*; D010105:Oxygenases / Q000235:genetics*
D016895:Culture Media, Serum-Free; D010078:Oxazines; C007649:ethoxyresorufin; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D010105:Oxygenases; D019363:Cytochrome P-450 CYP1A1