[Modification of Memebrane Lipid composition by lipophilic agents, and its effect on the activity of membrane-bound enzymes].
Rat jejunum was perfused for two and a half hours with aqueous ethanol (volume fraction 0.05) or aqueous carbon tetrachloride (volume fraction 0.005). Membrane vesicles subsequently prepared from the brush border displayed markedly decreased contents of cholesterol and phospholipids, as well as decreases in the activities of membrane-bound enzymes. These decreases were more pronounced in vesicles prepared after carbon tetrachloride than after ethanol perfusion. It is suggested that the anchoring of membrane-bound enzymes is decreased by physical chemical alterations in the hydrophobic region of the membrane.
Bossmann
B
B
Institut für Pathologische Biochemie, Martin-Luther-Universität Halle-Wittenberg, Halle/Saale.
Hütter
H J
HJ
D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology*; D002784:Cholesterol / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D006867:Hydrolases / Q000378:metabolism; D007583:Jejunum / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008871:Microvilli; D010477:Perfusion; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014166:Transferases / Q000378:metabolism
D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D000431:Ethanol; D002784:Cholesterol; D002251:Carbon Tetrachloride; D014166:Transferases; D006867:Hydrolases; D010450:Endopeptidases
Beeinflussung membrangebundener Enzymaktivitäten durch Veränderung der Membranlipidzusammensetzung.