Stimulation of glycolysis by corticotropin and phorbol ester in cultured neurons.
Incubation of cultured neurons from chick embryo forebrain with corticotropin (ACTH) or the phorbol ester TPA (12-O-tetradecanoylphorbol 13-acetate) stimulates the production of lactate. The stimulation is seen after 2 h of treatment and is maximal after 12 h. Both ACTH (1-24) and TPA increase the concentration of fructose 2,6-bisphosphate (Fru-2,6-P2), a metabolic activator of 6-phosphofructo-1-kinase (PFK-1). This effect is concentration-dependent and is maximal after 4 h of treatment. PFK-1 activity is increased in a dose-dependent manner by ACTH (1-24) or TPA. This increase is not visible during the first 6 h and reaches its maximum after 18 h of treatment. The stimulation of PFK-1 activity is not due the increase of Fru-2,6-P2 by ACTH (1-24) or TPA, since saturating concentrations of Fru-2,6-P2 are present in the PFK-1 assay medium. Thus, it appears that ACTH (1-24) and TPA regulate glycolysis through two modes with different time responses: increase in Fru-2,6-P2 is the main mechanism operating during the first 6 h following the treatments and increase in the amount, or stable increase in activity of PFK-1, takes place during the later phase. It is suggested that the action of corticotropin on glycolysis is part of the mechanism of the neurotrophic activity of this hormone.
Anglard
P
P
Centre de Neurochimie du CNRS, Strasbourg, France.
Magal
E
E
Louis
J C
JC
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003366:Cosyntropin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005635:Fructosediphosphates / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D007773:Lactates / Q000096:biosynthesis; D009474:Neurons / Q000187:drug effects*; D010703:Phorbol Esters / Q000494:pharmacology*; D010732:Phosphofructokinase-1 / Q000187:drug effects; D016548:Prosencephalon / Q000187:drug effects*; D013997:Time Factors
D005635:Fructosediphosphates; D007773:Lactates; D010703:Phorbol Esters; D003366:Cosyntropin; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1; D005947:Glucose