Selection of mouse cells with amplified metallothionein genes retaining their glucocorticoid inducibility.
Two new mouse cell mutants, resistant to either 80 or 100 mM CdCl2, were isolated to study the regulation of transcription by the glucocorticoid hormones. Their metallothionein mt-1% and mt-2+ genes were amplified coordinately to a maximum of 30 copies per cell. By Southern blot analysis, no gross rearrangement was detectable near the mt+ loci. Contrary to other mutants previously isolated, the metallothionein-specific mRNAs of these mutants are inducible by dexamethasone.
Thibodeau
J
J
Department de Microbiologie, Faculté de Médecine, Université de Sherbrooke, Qué, Canada.
De Souza
C
C
Smorawinska
M
M
Thirion
J P
JP
D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D015139:Blotting, Southern; D002104:Cadmium / Q000494:pharmacology; D002478:Cells, Cultured; D004247:DNA; D003907:Dexamethasone / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005784:Gene Amplification; D015870:Gene Expression / Q000187:drug effects; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic
D012333:RNA, Messenger; D002104:Cadmium; D003907:Dexamethasone; D004247:DNA; D008668:Metallothionein