title: A possible involvement of central atrial natriuretic peptide in cerebral cortical microcirculation.
abstract: The possible involvement of atrial natriuretic peptide (ANP) in cerebral cortical microcirculation was investigated in rats by means of laser-Doppler flowmetry and immunohistochemistry. In the laser-Doppler study, local cerebral blood flow (LCBF) changes after the administration of 10(-6) to 10(-8) mol/LANP solution or vehicle (saline solution) as an intracortical injection for 5 minutes were continuously monitored throughout the 30 minutes of the study and were expressed as percentages of preinjection values represented as 0%. The administration of 10(-6) to 10(-8) mol/LANP caused a significant decrease in LCBF; the onset of LCBF responses occurred within a few minutes after the start of the injection and the decrease in LCBF reached the maximum level within 7 to 10 minutes after the completion of the administration, after which LCBF gradually recovered. In the immunohistochemical study, no specific ANP immunoreactivity was found associated with the intraparenchymal blood vessels; however, ANP-immunoreactive neurons were observed primarily in the hypothalamus and septum, in which high concentrations of ANP-containing neurons have been identified. The data from the laser-Doppler study suggest that central ANP may produce a vasoconstriction of the intraparenchymal blood vessels, regardless of whether through direct action on these vessels or through the mediation by some system in the central nervous system. Because there is no evidence for ANP-containing nerves around these vessels, the role of central ANP in the cerebral circulation must await identification of the source of perivascular ANP.
authors.affiliation: Department of Neurological Surgery, Wakayama Medical College, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Nakao
authors.forename: T
authors.initials: T
authors.lastname: Itakura
authors.forename: Y
authors.initials: Y
authors.lastname: Uematsu
authors.forename: H
authors.initials: H
authors.lastname: Yokote
authors.forename: K
authors.initials: K
authors.lastname: Nakai
authors.forename: N
authors.initials: N
authors.lastname: Komai
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis / Q000502:physiology*; D001783:Blood Flow Velocity; D002540:Cerebral Cortex / Q000098:blood supply; D002560:Cerebrovascular Circulation / Q000502:physiology*; D007124:Immunoenzyme Techniques; D008297:Male; D008833:Microcirculation; D009474:Neurons / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D014661:Vasoconstriction / Q000502:physiology*
chemical_list: D009320:Atrial Natriuretic Factor