Role of fos-AP-1 binding sequence (FAP) in the induction of c-fos expression by purified C-kinase and in c-fos down-regulation following serum induction.
Microinjection of purified calcium phospholipid-dependent protein kinase (C-kinase) resulted in the rapid and transient induction of c-fos in quiescent rat embryo fibroblats. This C-kinase-induced expression of c-fos was prevented by in vivo competition using co-injection of oligonucleotides corresponding to the sequence of either the serum response element (SRE) or the fos AP-1 binding sequence (FAP) adjacent to SRE. This indicates that both these sequences must be involved in the binding/activation of protein factors required for the induction of c-fos by C-kinase. In contrast, the induction of c-fos by serum or by casein kinase II microinjection, which is also inhibited by injection of SRE oligonucleotides, is only delayed and then markedly prolonged by injecting TRE/FAP sequence, demonstrating that the FAP site plays a prominent role in vivo in the down-regulation of the endogenous c-fos gene expression.
Gauthier-Rouvière
C
C
CRBM, CNRS/INSERM, Montpellier, France.
Basset
M
M
Lamb
N J
NJ
Fernandez
A
A
D000818:Animals; D001483:Base Sequence; D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media; D015536:Down-Regulation; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D016762:Genes, fos; D066298:In Vitro Techniques; D008845:Microinjections; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage / Q000737:chemistry; D011493:Protein Kinase C / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics* / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid
D003470:Culture Media; D009838:Oligodeoxyribonucleotides; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D011493:Protein Kinase C