title: Functional implications of interleukin-1 beta based on the three-dimensional structure.
abstract: The molecular structure of interleukin-1 beta, a hormone-like cytokine with roles in several disease processes, has been determined at 2.0 A resolution and refined to a crystallographic R-factor of 0.19. The framework of this molecule consists of 12 antiparallel beta-strands exhibiting pseudo-3-fold symmetry. Six of the strands make up a beta-barrel with polar residues concentrated at either end. Analysis of the three-dimensional structure, together with results from site-directed mutagenesis and biochemical and immunological studies, suggest that the core of the beta-barrel plays an important functional role. A large patch of charged residues on one end of the barrel is proposed as the binding surface with which IL-1 interacts with its receptor.
authors.affiliation: Center for Advanced Research in Biotechnology, University of Maryland, Rockville.
authors.forename: B
authors.initials: B
authors.lastname: Veerapandian
authors.forename: G L
authors.initials: GL
authors.lastname: Gilliland
authors.forename: R
authors.initials: R
authors.lastname: Raag
authors.forename: A L
authors.initials: AL
authors.lastname: Svensson
authors.forename: Y
authors.initials: Y
authors.lastname: Masui
authors.forename: Y
authors.initials: Y
authors.lastname: Hirai
authors.forename: T L
authors.initials: TL
authors.lastname: Poulos
mesh_terms: D006801:Humans; D006860:Hydrogen Bonding; D007375:Interleukin-1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D013329:Structure-Activity Relationship
chemical_list: D007375:Interleukin-1