title: Sequences of the botulinal neurotoxin E derived from Clostridium botulinum type E (strain Beluga) and Clostridium butyricum (strains ATCC 43181 and ATCC 43755).
abstract: Recently, it has been shown that two Clostridium butyricum strains (ATCC 43181 and ATCC 43755), isolated from cases of infant botulism, produce a botulinal neurotoxin type E (BoNT/E). Here we have determined the nucleotide sequences of the BoNT/E genes of these two C. butyricum strains and from C. botulinum E strain Beluga. We show that the sequences of the BoNT/E genes from the two C. butyricum strains are identical and differ in only 64 positions resulting in 39 amino acid changes (97% identity at the amino acid level) from that derived from C. botulinum. Our data suggest a transfer of the BoNT/E gene from C. botulinum to the originally nontoxigenic C. butyricum strains.
authors.affiliation: Unité des Antigènes Bactériens, Institut Pasteur, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Poulet
authors.forename: D
authors.initials: D
authors.lastname: Hauser
authors.forename: M
authors.initials: M
authors.lastname: Quanz
authors.forename: H
authors.initials: H
authors.lastname: Niemann
authors.forename: M R
authors.initials: MR
authors.lastname: Popoff
mesh_terms: D000595:Amino Acid Sequence; D001905:Botulinum Toxins / Q000235:genetics*; D003013:Clostridium / Q000235:genetics*; D003014:Clostridium botulinum / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001905:Botulinum Toxins