title: Characterization of a polyclonal anti-peptide antibody to the angiotensin II type-1 (AT1) receptor.
abstract: A polyclonal antibody has been prepared against a synthetic peptide corresponding to amino acids 14-23 of the angiotensin II type-1 (AT1) receptor. The antibody is of high titer and mono-specific. Western blot analysis of membranes from rat liver, kidney, and adrenal gland showed that the antibody specifically recognizes a protein band of MW 70,000 whose amounts are highest in the liver, followed by kidney and adrenals. In addition, a relatively less prominent band of MW 95,000 was also detected. The relative distribution of this protein correlates well with the values obtained for [3H]-DuP753 binding and AT1 receptor mRNA.
authors.affiliation: Department of Physiology, College of Medicine, University of Florida, Gainesville 32610.
authors.forename: B
authors.initials: B
authors.lastname: Zelezna
authors.forename: E M
authors.initials: EM
authors.lastname: Richards
authors.forename: W
authors.initials: W
authors.lastname: Tang
authors.forename: D
authors.initials: D
authors.lastname: Lu
authors.forename: C
authors.initials: C
authors.lastname: Sumners
authors.forename: M K
authors.initials: MK
authors.lastname: Raizada
mesh_terms: D000311:Adrenal Glands / Q000737:chemistry; D000804:Angiotensin II / Q000378:metabolism*; D000910:Antibodies, Heterophile; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008566:Membranes / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis* / Q000276:immunology*; D011945:Receptors, Angiotensin / Q000032:analysis* / Q000276:immunology*
chemical_list: D000910:Antibodies, Heterophile; D010446:Peptide Fragments; D011945:Receptors, Angiotensin; D000804:Angiotensin II