Blockade of transgenic gamma delta T cell development in beta 2-microglobulin deficient mice.
The gamma delta T cell receptor (TCR) of the hybridoma KN6 recognizes the self molecule encoded by a class I gene which maps within the TL region of the major histocompatibility complex (MHC) of H-2b mice. Mice transgenic (Tg) for this TCR were crossed with mice genetically deficient in beta 2-microglobulin (beta 2m). No mature Tg gamma delta T cells were detected in the thymus or the spleen of the beta 2m- gamma delta Tg mice. We conclude that interaction between the Tg gamma delta TCR and a beta 2m-associated molecule (probably an MHC class I molecule) is required for the generation of mature Tg gamma delta T cells.
Pereira
P
P
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139.
Zijlstra
M
M
McMaster
J
J
Loring
J M
JM
Jaenisch
R
R
Tonegawa
S
S
D000818:Animals; D030801:Animals, Genetically Modified; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000276:immunology; D001483:Base Sequence; D051927:CD24 Antigen; D002454:Cell Differentiation; D003433:Crosses, Genetic; D004622:Embryo, Mammalian / Q000276:immunology; D015870:Gene Expression; D005838:Genotype; D006239:Haplotypes; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics* / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology; D001613:beta 2-Microglobulin / Q000172:deficiency / Q000235:genetics*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D051927:CD24 Antigen; C497890:Cd24a protein, mouse; D008562:Membrane Glycoproteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D001613:beta 2-Microglobulin