Polygenic and single gene responses to selection for resistance to diazinon in Lucilia cuprina.
Following mutagenesis with ethyl methanesulfonate, selection in a susceptible strain with a concentration of the insecticide diazinon (0.0004%, w/v) above that required to kill 100% of the susceptible strain, the LC100 of that strain, resulted in a single gene response. The resultant four mutant resistant strains have equivalent physiological, genetical and biochemical profiles to a diazinon-resistant strain derived from a natural population and homozygous for the Rop-1 allele. Modification of the microsomal esterase E3 is responsible for resistance in each case. The Rop-1 locus maps approximately 4.4 map units proximal to bu on chromosome IV. Selection within the susceptible distribution, at a concentration of diazinon [0.0001% (w/v)] less than the LC100, resulted in a similar phenotypic response irrespective of whether the base population had been mutagenized. The responses were polygenically based, unique to each selection line and independent of Rop-1. The relevance of the results to selection for insecticide resistance in laboratory and natural populations is discussed.
McKenzie
J A
JA
Department of Genetics, University of Melbourne, Parkville, Australia.
Parker
A G
AG
Yen
J L
JL
D000818:Animals; D002874:Chromosome Mapping; D003976:Diazinon / Q000494:pharmacology*; D004175:Diptera / Q000187:drug effects / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007305:Insecticide Resistance / Q000235:genetics; D008297:Male; D010641:Phenotype; D012641:Selection, Genetic
D003976:Diazinon