Charge effect in the interaction of doxorubicin and derivatives with polydeoxynucleotides.
The equilibrium interaction of doxorubicin and its N-acetyl derivative with a series of purine-pyrimidine alternating polydeoxynucleotides has been studied through spectrofluorometry to assess the relevance of the electrostatic contribution to DNA intercalation. The results have shown that: (a) the suppression of the positive charge on the aminosugar has: (I) a profound negative effect on the free energy of intercalation, as expected, and (II) a negligible influence on the base specificity, which supports the notion of an essentially electrostatic effect of N-acetylation on intercalation; (b) a reasonably good accord with the demands of a polyelectrolytic model, due to Friedman and Manning, is found.
Ruggiero
J
J
Physics Department, Institute of Biosciences, UNESP, São José do Rio Preto, Brazil.
Xodo
L E
LE
Ciana
A
A
Manzini
G
G
Quadrifoglio
F
F
D004247:DNA / Q000378:metabolism*; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000378:metabolism*; D004560:Electricity; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D004317:Doxorubicin; D004247:DNA