title: Sequence and characterization of the met-7 gene of Neurospora crassa.
abstract: The proteins encoded by the met-7+ and met-3+ genes of Neurospora crassa are required to form a functional cystathionine-gamma-synthase (CGS). The met-7+ gene has been cloned by complementation of a met-7 mutant. The nucleotide sequence of the complementing DNA reveals the presence of a 542-amino acid open reading frame (ORF). Disruption of this ORF abolishes complementation of the met-7 mutation.
authors.affiliation: Department of Genetics, University of Georgia, Athens 30602.
authors.forename: J M
authors.initials: JM
authors.lastname: Crawford
authors.forename: R F
authors.initials: RF
authors.lastname: Geever
authors.forename: D K
authors.initials: DK
authors.lastname: Asch
authors.forename: M E
authors.initials: ME
authors.lastname: Case
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D019757:Carbon-Oxygen Lyases; D003001:Cloning, Molecular; D005816:Genetic Complementation Test; D008190:Lyases / Q000235:genetics*; D008715:Methionine / Q000235:genetics*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics
chemical_list: D008715:Methionine; C022696:O-succinylhomoserine (thiol)-lyase; D008190:Lyases; D019757:Carbon-Oxygen Lyases