title: Localization of the basement membrane heparan sulfate proteoglycan in islet amyloid deposits in type II diabetes mellitus.
abstract: Amyloid in the islets of Langerhans is the uniform pathologic feature in the pancreata of patients with type II diabetes mellitus. Although the mechanisms of islet amyloid fibrillogenesis are unknown, the presence of heparan sulfate proteoglycan in many other forms of amyloid suggests a role for this proteoglycan in amyloidogenesis in general. In this study, islet amyloid was evaluated for the presence of the basement membrane heparan sulfate proteoglycan using histochemical and immunohistochemical techniques. Staining with sodium sulfate-alcian blue identified highly sulfated glycosaminoglycans within all islet amyloid deposits, and anti-basement membrane heparan sulfate proteoglycan antisera localized this specific proteoglycan within the islet amyloid. The presence of the basement membrane heparan sulfate proteoglycan links islet amyloid to other disparate forms of amyloid and further supports the hypothesis that it has a role in a common pathway of amyloid fibrillogenesis.
authors.affiliation: Department of Pathology, Queen's University, Kingston, Ontario, Canada.
authors.forename: I D
authors.initials: ID
authors.lastname: Young
authors.forename: L
authors.initials: L
authors.lastname: Ailles
authors.forename: S
authors.initials: S
authors.lastname: Narindrasorasak
authors.forename: R
authors.initials: R
authors.lastname: Tan
authors.forename: R
authors.initials: R
authors.lastname: Kisilevsky
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D001485:Basement Membrane / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000737:chemistry*; D011509:Proteoglycans / Q000032:analysis*
chemical_list: D000682:Amyloid; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D011509:Proteoglycans