title: Paired comparison of platelet concentrates prepared from platelet-rich plasma and buffy coats using a new technique with 111In and 51Cr.
abstract: Two techniques for the preparation of platelet concentrate (PC), the standard platelet-rich plasma (PRP) and buffy coat (BC) methods, were compared in nine paired studies with regard to platelet harvest, white cell (WBC) contamination, and PC quality after 5 days of 22 degrees C storage. Platelet harvest using the BC method averaged approximately 56 percent of the whole blood level (6.2 x 10(10)/concentrate), which was less than the 76 percent achieved with the PRP-PC method (8.7 x 10(10)/concentrate). An additional 5 units collected into an experimental siphon bag for BC-PC processing showed improved platelet harvest (6.7 x 10(10)/concentrate, or approx. 70% of whole blood). WBCs remaining in the BC-PC averaged 0.19 x 10(8) per unit compared to 3.6 x 10(8) per unit for PRP-PC. Buffy coat processing produced red cell (RBC) units with 50 percent of the WBC contamination of conventionally prepared units (9.8 +/- 6.2 x 10(8)/unit vs. 18.9 +/- 7.1 x 10(8)/unit). The siphon bag further reduced WBC levels in the AS-3 RBC units (6.4 +/- 3.7 x 10(8)/unit). In vitro studies performed on Days 1 and 5 after collection showed no significant differences in platelet metabolic and biologic function or cell integrity. Beta-thromboglobulin and surface glycoprotein levels, indicators of platelet activation and membrane alteration, respectively, did not differ significantly in the PRP-PC and BC-PC; nor was lactate production higher in PRP-PC, despite the substantially higher WBC counts. Autologous in vivo platelet viability determinations were performed by using concurrent transfusion of 111In-labeled freshly drawn platelets and 51Cr-labeled stored platelets.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: American Red Cross, Mid-Atlantic Regional Blood Services, Norfolk, Virginia.
authors.forename: T
authors.initials: T
authors.lastname: Keegan
authors.forename: A
authors.initials: A
authors.lastname: Heaton
authors.forename: S
authors.initials: S
authors.lastname: Holme
authors.forename: M
authors.initials: M
authors.lastname: Owens
authors.forename: E
authors.initials: E
authors.lastname: Nelson
authors.forename: R
authors.initials: R
authors.lastname: Carmen
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D002860:Chromium Radioisotopes; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D007205:Indium Radioisotopes; D007773:Lactates / Q000097:blood; D007962:Leukocytes / Q000166:cytology; D010976:Platelet Count; D010983:Plateletpheresis / Q000295:instrumentation / Q000379:methods*; D011865:Radioisotope Dilution Technique
chemical_list: D002860:Chromium Radioisotopes; D007205:Indium Radioisotopes; D007773:Lactates