title: Shigella-specific IgA in saliva of children with bacillary dysentery.
abstract: To study the secretory immune response after Shigella infection, the anti-lipopolysaccharide and anti-Shiga-toxin response in saliva, obtained from children with confirmed shigellosis and healthy children, were determined by enzyme-linked immunosorbent assay and by Western blot. Children with infection showed high titers compared to healthy controls. After Shigella dysenteriae type 1 infection a significant change in titer could be observed in a large number of cases, in contrast to Shigella flexneri infection. It appeared that, in children living in endemic areas, infection with one serotype can give a rise in antibody titer to another serotype. This could be ascribed to polyclonal B cell activation since children in endemic areas routinely show relatively high titers to Shigella antigens. We conclude that the dynamics of salivary anti-Shigella LPS and anti-Shiga-toxin in children with dysentery indicate that it can be applied to studies of immune response in shigellosis for epidemiological and vaccination purposes.
authors.affiliation: Department of Medical Microbiology, Academical Medical Centre, Amsterdam, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Schultsz
authors.forename: F
authors.initials: F
authors.lastname: Qadri
authors.forename: S A
authors.initials: SA
authors.lastname: Hossain
authors.forename: F
authors.initials: F
authors.lastname: Ahmed
authors.forename: I
authors.initials: I
authors.lastname: Ciznar
mesh_terms: D001427:Bacterial Toxins / Q000276:immunology; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D004405:Dysentery, Bacillary / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D012463:Saliva / Q000276:immunology*; D022603:Shiga Toxins; D012762:Shigella dysenteriae / Q000276:immunology*; D012763:Shigella flexneri / Q000276:immunology*
chemical_list: D001427:Bacterial Toxins; D007071:Immunoglobulin A, Secretory; D008070:Lipopolysaccharides; D022603:Shiga Toxins