title: Phosphoglycerate mutase from Streptomyces coelicolor A3(2): purification and characterization of the enzyme and cloning and sequence analysis of the gene.
abstract: The enzyme 3-phosphoglycerate mutase was purified 192-fold from Streptomyces coelicolor, and its N-terminal sequence was determined. The enzyme is tetrameric with a subunit Mr of 29,000. It is 2,3-bisphosphoglycerate dependent and inhibited by vanadate. The gene encoding the enzyme was cloned by using a synthetic oligonucleotide probe designed from the N-terminal peptide sequence, and the complete coding sequence was determined. The deduced amino acid sequence is 64% identical to that of the phosphoglycerate mutase of Saccharomyces cerevisiae and has substantial identity to those of other phosphoglycerate mutases.
authors.affiliation: Department of Biochemistry, University of Glasgow, United Kingdom.
authors.forename: P J
authors.initials: PJ
authors.lastname: White
authors.forename: J
authors.initials: J
authors.lastname: Nairn
authors.forename: N C
authors.initials: NC
authors.lastname: Price
authors.forename: H G
authors.initials: HG
authors.lastname: Nimmo
authors.forename: J R
authors.initials: JR
authors.lastname: Coggins
authors.forename: I S
authors.initials: IS
authors.lastname: Hunter
mesh_terms: D019794:2,3-Diphosphoglycerate; D000595:Amino Acid Sequence; D001483:Base Sequence; D001731:Bisphosphoglycerate Mutase / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000302:isolation & purification; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004163:Diphosphoglyceric Acids / Q000737:chemistry; D005798:Genes, Bacterial; D005988:Glyceric Acids / Q000737:chemistry; D007700:Kinetics; D008969:Molecular Sequence Data; D011487:Protein Conformation; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D013379:Substrate Specificity; D014638:Vanadates / Q000494:pharmacology
chemical_list: D004163:Diphosphoglyceric Acids; D005988:Glyceric Acids; D019794:2,3-Diphosphoglycerate; D014638:Vanadates; C005156:3-phosphoglycerate; D001731:Bisphosphoglycerate Mutase