Unique and overlapping pollutant stress proteins of Escherichia coli.
Exposure of growing batch cultures of Escherichia coli to nine different "model micropollutants" (benzene, cadmium chloride, chlorpyrivos, 2,4-dichloroaniline, dioctylphtalate, hexachlorobenzene, pentachlorophenol, trichloroethylene, and tetrapropylbenzosulfonate) led to the induction of 13 to 39 proteins, as analyzed by two-dimensional gel electrophoresis. Some of these proteins overlapped with heat shock and carbon starvation proteins, but at least 50% were unique to a given chemical. The stress protein induction showed a temporal pattern, indicating sequential gene expression. Chemical stress protein synthesis occurred even at concentrations that had no effect on growth. Thus, the synthesis of these proteins can be a sensitive index of stress and the nature of environmental pollution.
Blom
A
A
Department of Biology, TNO Institute of Environmental Sciences, Delft, The Netherlands.
Harder
W
W
Matin
A
A
D001426:Bacterial Proteins / Q000096:biosynthesis*; D015180:Electrophoresis, Gel, Two-Dimensional; D004785:Environmental Pollutants / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis*
D001426:Bacterial Proteins; D004785:Environmental Pollutants; D006360:Heat-Shock Proteins