Interaction of p107 with cyclin A independent of complex formation with viral oncoproteins.
The p107 protein and the retinoblastoma protein (RB) both bind specifically to two viral oncoproteins, the SV40 T antigen (T) and adenoviral protein E1A (E1A). Like RB, p107 contains a segment (the pocket) that, alone, can bind specifically to T, E1A, and multiple cellular proteins. Cyclin A bound to the p107 pocket, but not the RB pocket. Although both pockets contain two, related collinear subsegments (A and B), the unique sequence in the p107 pocket that occupies the space between A and B is required for the interaction with cyclin A.
Ewen
M E
ME
Dana-Farber Cancer Institute, Boston, MA 02115.
Faha
B
B
Harlow
E
E
Livingston
D M
DM
D017456:Adenovirus Early Proteins; D000595:Amino Acid Sequence; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D003001:Cloning, Molecular; D016213:Cyclins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005134:Eye Neoplasms; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012175:Retinoblastoma; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism*; D050720:Retinoblastoma-Like Protein p107; D013329:Structure-Activity Relationship
D017456:Adenovirus Early Proteins; D000952:Antigens, Polyomavirus Transforming; D016213:Cyclins; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D009856:Oncogene Proteins, Viral; D011506:Proteins; C493844:RBL1 protein, human; D011993:Recombinant Fusion Proteins; D016160:Retinoblastoma Protein; D050720:Retinoblastoma-Like Protein p107; D005982:Glutathione Transferase