Class-specific antibodies to bovine respiratory syncytial virus in experimentally infected lambs.
Enzyme-linked immunoabsorbent assay (ELISA) was used to titrate virus-specific IgG, IgM and IgA levels in nasal secretions, lung lavage fluids and serum samples sequentially obtained from lambs experimentally infected with bovine respiratory syncytial virus (RSV). Virus-specific IgG and IgM responses were measured by the indirect double antibody sandwich ELISA using anti-bovine RSV monoclonal antibody, as capture antibody, and peroxidase-conjugated anti-sheep IgG and anti-sheep IgM. Virus-specific IgA antibodies were measured by antibody capture assay using anti-sheep IgA (alpha-chain specific) and anti-bovine RSV monoclonal antibodies. Bovine RSV-specific IgM and IgA antibodies were detected in the serum samples within 6 days post-inoculation (p.i.). Virus-specific IgC antibodies appeared in serum samples 4 days later. In nasal secretions, IgA antibodies appeared 7 days p.i. but IgM antibodies were not detected until 12-16 days p.i. In serum samples, IgM titres were predominant for the first 2 weeks p.i. IgC titres becoming predominant thereafter. In nasal secretions and lung lavage fluids, IgA titres were significantly higher than IgM or IgG titres up to 21 days p.i. (0.01).
Sharma
R
R
University of Liverpool, Department of Veterinary Pathology, Neston, Wirral, UK.
Woldehiwet
Z
Z
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D007070:Immunoglobulin A / Q000032:analysis / Q000096:biosynthesis / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis / Q000097:blood; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis / Q000097:blood; D007136:Immunoglobulins / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D008168:Lung / Q000276:immunology; D009297:Nasal Mucosa / Q000276:immunology; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D010253:Respirovirus Infections / Q000276:immunology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins