[Effect of ribonuclease on cell cycle dynamics in tumor cells in vitro and in vivo].
The effects of RNAase from Bacteroides intermedius and pancreatic RNAase on the dynamics of cellular cycle phases were studied in the cells of ascitic lympholeukemia NK/Ly in vivo and in vitro, in the cells of the human ovary carcinoma CaOv and in the cells of ascitic leukemia P388 in vivo. It was shown that both the RNAases induced cell accumulation (blocking) in the G2/M phase of a cellular cycle with simultaneous certain depletion of the proportion of DNA-synthesizing cells (S-phase). In in vitro experiments, there was a higher efficiency of pancreatic RNAase. In animal experiments, RNAase from B. intermedius was more efficient. The likely target of the in vitro and in vivo effects of the enzymes on the cellular cycle dynamics and biological activity are discussed.
Sergeeva
E V
EV
Nikolaeva
T G
TG
Kurinenko
B M
BM
Dobrynin
Ia V
IaV
D000818:Animals; D001407:Bacillus / Q000201:enzymology; D002453:Cell Cycle / Q000187:drug effects*; D005260:Female; D006801:Humans; D007942:Leukemia, Experimental / Q000188:drug therapy*; D051379:Mice; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D012260:Ribonucleases / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D012260:Ribonucleases
Vliianie ribonukleaz na dinamiku kletochnogo tsikla opukholevykh kletok in vitro i in vivo.