Efficient inoculation with CaMV 35 S promoter-driven DNA clones of the tobravirus PEBV.
Clones have been constructed containing full-length cDNA copies of PEBV RNA1 and RNA2, flanked by the CaMV 35 S RNA promoter and the nopaline synthase terminator. The clones are infectious when inoculated onto Nicotiana benthamiana plants. Both the viral RNAs and the virus particles were identified in infected plants.
MacFarlane
S A
SA
Department of Virus Research, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, United Kingdom.
Gilmer
D
D
Davies
J W
JW
D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004252:DNA Mutational Analysis; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010942:Plant Viruses / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D004247:DNA