The effect of growth factor formula (platelet derived wound healing formula) in experimental spinal cord injuries.
The main purpose of this study was to investigate the usefulness of platelet derived wound healing formula (PDWHF) in the treatment of experimentally induced spinal cord injuries in cats. The injury model in ten adult cats consisted of the placement of three 22-gauge Teflon catheter sheaths into the spinal cord at the L2 level. Treatment consisted of coating these sheaths with PDWHF in Hydron. Three animals were used as controls. In the 7 remaining cats, treatment was double blinded. Cats were sacrificed at 3 weeks after injury and injured spinal cord segments were excised for histologic evaluation. Electrophysiologic and clinical motor function were evaluated throughout a period of observation. Evoked potentials in both the treated and control groups indicated incomplete spinal cord lesions due to insertion of the needles. There did not appear to be any significant improvement or difference in the evoked response and clinical function as a result of treatment with PDWHF. The histological findings in the PDWHF-treated group showed significant new vessel formation as well as dilation and around the injury site. This neovascularization, both qualitative and quantitative, was noted in the treatment group. This information, with a limited injury and very simple delivery system for growth factor, would suggest there is definite neovascularization occurring as a result of this treatment and this may be useful in the subsequent wound healing response such as axonal growth and scar tissue formation.
Hiraizumi
Y
Y
Department of Orthopaedic Surgery, University of Minnesota, Minneapolis 55455.
Transfeldt
E E
EE
Kawahara
N
N
Fiegel
V D
VD
Knighton
D
D
Sung
J H
JH
D000818:Animals; D002415:Cats; D004594:Electrophysiology; D006133:Growth Substances / Q000627:therapeutic use*; D013116:Spinal Cord / Q000473:pathology / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D014945:Wound Healing
D006133:Growth Substances