title: A novel mutation FruS, altering synthesis of components of the phosphoenolpyruvate: fructose phosphotransferase system in Escherichia coli K12.
abstract: A novel mutation, FruS localised in the fru operon was obtained. It uncouples expression of the genes determining synthesis of the fructose-specific transport proteins and fructose-1-phosphate kinase. In FruS bacteria the fruA and fruF genes (coding for Enzyme IIfru and FPr, respectively) are constitutive by expressed while fruK (encoding fructose-1-phosphate kinase) remains inducible. In contrast to other mutations, which render expression of the whole fru operon constitutive, the FruS mutation: (1) does not lead to D-xylitol sensitivity; (2) does not inhibit growth on D-lactate, pyruvate and L-alanine; (3) does not decrease phosphoenolpyruvate (PEP) synthase activity.
authors.affiliation: N.F. Gamaleya Institute for Epidemiology and Microbiology, Academy of Medical Sciences, Moscow, USSR.
authors.forename: T N
authors.initials: TN
authors.lastname: Bolshakova
authors.forename: M L
authors.initials: ML
authors.lastname: Molchanova
authors.forename: R S
authors.initials: RS
authors.lastname: Erlagaeva
authors.forename: Y A
authors.initials: YA
authors.lastname: Grigorenko
authors.forename: V N
authors.initials: VN
authors.lastname: Gershanovitch
mesh_terms: D000409:Alanine / Q000378:metabolism; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D005838:Genotype; D007773:Lactates / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D010732:Phosphofructokinase-1 / Q000235:genetics / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D014161:Transduction, Genetic; D014993:Xylitol / Q000378:metabolism
chemical_list: D007773:Lactates; D011773:Pyruvates; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D010732:Phosphofructokinase-1; C068652:1-phosphofructokinase; D000409:Alanine; D008358:Mannose; D014993:Xylitol