Hierarchy of responsiveness in vivo and in vitro among T cells expressing distinct Mls-1a-reactive v beta domains.
In this report we have compared responses of T cells bearing different T cell receptor (TcR) V beta domains, including V beta 6, 7, 8.1 and 9, to the minor lymphocyte stimulatory locus (Mls-1a). The virtually complete deletion of peripheral T cells using these TcR V beta domains in mice expressing Mls-1a verified their Mls-1a reactivity. However, varied responses among these cells following stimulation with Mls-1a in vitro suggested a hierarchy of TcR reactivities for Mls-1a with V beta 6 and V beta 9 greater than V beta 8.1 much greater than V beta 7. This hierarchy was further supported by in vivo studies which showed that V beta 6+CD4+ cells dominated responses to Mls-1a.
Waanders
G A
GA
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
McDonald
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HR
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D016747:Minor Lymphocyte Stimulatory Antigens / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D016747:Minor Lymphocyte Stimulatory Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta