title: The direct binding of an HIV fragment with granulocytes from healthy subjects and infected patients.
abstract: The binding of the synthetic fragment from the CD4 binding site of HIV (gp 120) with polymorphonuclear neutrophils (PMN) in 13 healthy donors and 31 HIV-infected patients was studied using a biotin-streptavidin-texas-red complex. The largest percentage of PMN-bound peptide was reached at a final peptide concentration of 10 micrograms/ml. The increase of peptide concentration did not raise the per cent of positive PMN. Preliminary incubation of PMN or mononuclear cells with non-biotinylated peptide abolished subsequent binding of these cells with biotinylated peptide, while preliminary treatment of the cells by anti-CD4 monoclonal antibody did not lead to such abrogation. It was revealed that about 14% of PMN but no lymphocytes from healthy donors were able to bind peptide. The number of such PMN in HIV-infected patients was significantly less (6.3 +/- 8.9%, P less than 0.05). A connection between peptide-bound PMN and their functional activity was found. The percentage of such cells was 13.0 +/- 11.5% in patients with normal values in a stimulated nitroblue tetrazolium reduction test and only 2.6 +/- 2.8% in patients with low values in this test (P less than 0.05).
authors.affiliation: Laboratory of AIDS Epidemiology and Prevention, Central Institute of Epidemiology, USSR Ministry of Public Health, Moscow.
authors.forename: D I
authors.initials: DI
authors.lastname: Gabrilovich
authors.forename: A T
authors.initials: AT
authors.lastname: Kozich
authors.forename: S A
authors.initials: SA
authors.lastname: Moshnikov
authors.forename: V V
authors.initials: VV
authors.lastname: Pokrovsky
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D015704:CD4 Antigens / Q000032:analysis / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology / Q000378:metabolism*; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D015658:HIV Infections / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D016131:Lymphocyte Subsets; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D010446:Peptide Fragments