Immunohistologic study of T-cell receptor delta-chain expression in rheumatoid synovial membranes.
Lymphocytes expressing gamma delta T-cell receptors (TCRs) have been shown to be reactive to mycobacterial antigens as well as the so-called stress proteins. The detection of increased numbers of gamma delta cells in the synovial fluid and peripheral blood of some patients with rheumatoid arthritis has suggested a potential role for these lymphocytes in the pathogenesis of this disorder. Twenty-three rheumatoid synovial membranes were studied using immunohistology and monoclonal antibodies in an attempt to define the patterns of distribution of gamma delta T cells in rheumatoid synovitis. Consecutive sections were stained for T1(CD5), T4(CD4), T8(CD8), TAC(CD25), the delta-chain markers delta TCR1 and delta TCS1, and the beta-chain marker beta F1. Our results show some regional differences in the distribution of CD4 and CD8 cells, the former being prominent in the lymphocytic aggregates and the latter most prominent in diffuse infiltrates immediately adjacent to the synovial lining layer. All tissues showed extensive staining for beta F1; an estimated average of more than 90% of T cells expressed alpha beta TCR. The majority of samples showed limited staining for both delta-chain antibodies, with 20 of the 23 tissues appearing to have less than 1% of T lymphocytes expressing these markers. Three tissues stained extensively for both delta TCR1 and delta TCS1 in particular areas of the section. In these areas, small perivascular lymphocytic aggregates appeared to be composed mainly of gamma delta cells. TAC staining was virtually absent in all areas and tissues. It was concluded that the majority of T lymphocytes infiltrating rheumatoid synovial membranes express alpha beta TCR.(ABSTRACT TRUNCATED AT 250 WORDS)
el-Gabalawy
H S
HS
Section of Rheumatology, University of Manitoba, Winnipeg, Canada.
Keillor
J
J
D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013583:Synovial Membrane / Q000737:chemistry*; D013601:T-Lymphocytes / Q000166:cytology
D016692:Receptors, Antigen, T-Cell, gamma-delta; D015375:Receptors, Interleukin-2