title: Identification of a major growth factor for AIDS-Kaposi's sarcoma cells as oncostatin M.
abstract: Conditioned medium from human T cell leukemia virus type 2 (HTLV-II)-infected T cells supports the growth and long-term culture of cells derived from acquired immunodeficiency syndrome (AIDS)-associated Kaposi's sarcoma lesions (AIDS-KS cells). A protein of 30 kilodaltons was purified from conditioned medium that supports the growth of AIDS-KS cells. The amino-terminal sequence of this protein was identical to the amino-terminal sequence of Oncostatin M, a glycoprotein that inhibits the growth of a variety of cancer cells. Oncostatin M from conditioned medium stimulated a twofold increase in the growth of AIDS-KS cells at a concentration of less than 1 nanogram of the protein per milliliter of medium.
authors.affiliation: Department of Cell Biology, Advanced BioScience Laboratories, Inc., Kensington, MD.
authors.forename: B C
authors.initials: BC
authors.lastname: Nair
authors.forename: A L
authors.initials: AL
authors.lastname: DeVico
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: T D
authors.initials: TD
authors.lastname: Copeland
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Patel
authors.forename: T
authors.initials: T
authors.lastname: O'Neil
authors.forename: S
authors.initials: S
authors.lastname: Oroszlan
authors.forename: R C
authors.initials: RC
authors.lastname: Gallo
authors.forename: M G
authors.initials: MG
authors.lastname: Sarngadharan
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000595:Amino Acid Sequence; D003470:Culture Media / Q000737:chemistry; D006133:Growth Substances / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D053683:Oncostatin M; D010455:Peptides / Q000302:isolation & purification / Q000502:physiology*; D012514:Sarcoma, Kaposi / Q000209:etiology / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media; D006133:Growth Substances; C508351:OSM protein, human; D010455:Peptides; D053683:Oncostatin M