Cloning and characterization of a sixth adenylyl cyclase isoform: types V and VI constitute a subgroup within the mammalian adenylyl cyclase family.
A sixth member of the mammalian adenylyl cyclase family has been isolated from a canine cardiac cDNA library. This isoform is more highly homologous to type V than to the other adenylyl cyclase types; sequence similarity is apparent even in the transmembrane regions where the greatest divergence among the types exists. Type VI mRNA expression is most abundant in heart and brain; however, unlike type V, a low level of expression is also observed in a variety of other tissues examined. Type VI adenylyl cyclase can be stimulated by NaF, guanosine 5'-[gamma-thio]triphosphate, and forskolin but not by Ca2+/calmodulin, whereas it is inhibited by adenosine and its analogues. Comparison of both their structural and biochemical properties suggests that types V and VI constitute a distinct subgroup of the mammalian adenylyl cyclase family.
Katsushika
S
S
Department of Pharmacology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Chen
L
L
Kawabe
J
J
Nilakantan
R
R
Halnon
N J
NJ
Homcy
C J
CJ
Ishikawa
Y
Y
D000241:Adenosine / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D003001:Cloning, Molecular; D004285:Dogs; D015870:Gene Expression; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D014018:Tissue Distribution; D014162:Transfection
D002147:Calmodulin; D012333:RNA, Messenger; D000262:Adenylyl Cyclases; D000241:Adenosine; D002118:Calcium