Amplification and rearrangement of melF/mouse CD43 (leukosialin) gene encoding a highly glycosylated membrane protein gp120 in Friend erythroleukemia cells.
Using a differential hybridization method, we isolated a cDNA (melF) overexpressed in a murine Friend erythroleukemia cell line, F5-5. Southern blotting and sequence analysis revealed that the melF cDNA encodes a membrane protein of 395 amino acids that is the couterpart to human CD43 (leukosialin) surface antigen of lymphocytes in mice. The melF/mouse CD43 (mCD43) gene was found to be amplified eight- to 10-fold not only in F5-5 cells, but also in their sib cell lines, including the earliest clone established. This indicates that the amplification of melF/mCD43 gene occurred at an early stage of malignant transformation of the F5-5 family. Furthermore, the same gene was rearranged in another Friend erythroleukemia cell line TSA8. In both cell lines, F5-5 and TSA8, the levels of melF/mCD43 mRNA were 30- to 50-fold higher than that in uninfected spleen cells. Among normal tissues examined, the expression of melF/mCD43 gene was restricted to hematopoietic tissues. These results suggest that melF/mCD43 gene has an important role in progression of leukemic cells in the erythroid lineage and also has a physiological function in normal hematopoiesis.
Misawa
Y
Y
Department of Genetics, University of Tokyo, Japan.
Shibuya
M
M
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D015321:Gene Rearrangement; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D051917:Leukosialin; D008099:Liver / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D015682:Retroviridae Proteins, Oncogenic; D012795:Sialoglycoproteins / Q000235:genetics*; D013154:Spleen / Q000378:metabolism
D015703:Antigens, CD; D004268:DNA-Binding Proteins; D051917:Leukosialin; D015682:Retroviridae Proteins, Oncogenic; D012795:Sialoglycoproteins; C497863:Spn protein, mouse; C062022:v-Spi-1 protein, Friend spleen focus-forming virus