Use of an indwelling bronchial catheter model of bovine pneumonic pasteurellosis for evaluation of therapeutic efficacy of various compounds.
A model of bovine pneumonic pasteurellosis, using an indwelling bronchial catheter for inoculation and subsequent lavage of a single main stem bronchus of the lung, was evaluated in a preliminary efficacy trial of an experimental therapeutic compound. Inoculation of 10(7) Pasteurella haemolytica organisms into the bronchus consistently induced a focal pneumonic lesion with typical morphology of pneumonic pasteurellosis in the left or right caudal lung lobe. The experimental treatment caused significant (P less than 0.05) reduction in lung lesion volume, compared with that of a saline-treated control. It also caused significant (P less than 0.05) reduction in lavage fluid bacterial counts at 48 hours after inoculation, compared with counts in the controls. The inflammatory cell count and the percentage of neutrophils increased markedly in lavage fluids 8 hours after inoculation, but differences were not detected between treatments. Significant differences between treatments were not found in clinical signs, rectal temperature, or histologic changes. This model appears to be a sensitive indicator of treatment efficacy and has the advantage over previous models of pneumonic pasteurellosis of allowing sequential monitoring of the primary lesion site.
Paulsen
D B
DB
Department of Veterinary Pathology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
Corstvet
R E
RE
McClure
J R
JR
Enright
F M
FM
McBride
J W
JW
McDonough
K C
KC
D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use; D000907:Antibodies, Bacterial / Q000097:blood; D001831:Body Temperature; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000382:microbiology; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D002939:Ciprofloxacin / Q000031:analogs & derivatives / Q000627:therapeutic use; D015169:Colony Count, Microbial / Q000662:veterinary; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D024841:Fluoroquinolones; D016978:Mannheimia haemolytica / Q000276:immunology* / Q000302:isolation & purification; D010118:Oxytetracycline / Q000627:therapeutic use; D012766:Pasteurellosis, Pneumonic / Q000188:drug therapy* / Q000473:pathology
D000890:Anti-Infective Agents; D000907:Antibodies, Bacterial; D024841:Fluoroquinolones; D002939:Ciprofloxacin; C047225:difloxacin; D010118:Oxytetracycline