title: Trans-acting factors and properly positioned DNA elements repress mating-type genes in fission yeast.
abstract: Repression of the mating-type P genes at the silent mat2-P locus in fission yeast is dependent on four cis-acting DNA elements, two on each side of the coding sequences. The mechanism by which these elements exert their influence on the mating-type promoter is studied here by insertion of a bacterial antibiotic resistance gene at several positions in the silent region. The behavior of the resistance gene itself, and the changes its insertion causes in mating-type expression, reveal that the repressive elements have a limited range of action and that the four elements have unequal effects on gene expression. Repression of the antibiotic resistance gene inside the silent region leads to an antibiotic-sensitive phenotype and facilitates the selection of resistant mutants. These mutants can de-repress the resistance gene at other positions than the one used for their selection. Strong antibiotic resistance correlates with derepression of the plasmid-borne mating-type cassette. These data argue that mat2-P repression is dependent on trans-acting factors and the positioning of the repressive DNA elements, but less dependent on the nature of the affected promoter.
authors.affiliation: Department of Molecular Biology, Uppsala University, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Ekwall
authors.forename: T
authors.initials: T
authors.lastname: Olsson
authors.forename: T
authors.initials: T
authors.lastname: Ruusala
mesh_terms: D000483:Alleles; D002843:Chromatin / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D005800:Genes, Fungal / Q000235:genetics*; D049770:Genes, Mating Type, Fungal; D007613:Kanamycin Resistance / Q000235:genetics; D009154:Mutation; D010957:Plasmids; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D002843:Chromatin; D012097:Repressor Proteins; D015534:Trans-Activators