The distribution of neural crest-derived Schwann cells from subsets of brachial spinal segments into the peripheral nerves innervating the chick forelimb.
Neural crest cells from brachial levels of the neural tube populate the ventral roots, spinal nerves, and peripheral nerves of the chick forelimb where they give rise to Schwann cells. The distribution of neural crest cells in the developing forelimb was examined using homotopic and heterotopic chick-quail chimeras to label neural crest cells from subsets of the brachial spinal segments. Neural crest cells from particular regions of the spinal cord populated ventral roots and spinal nerves adjacent to or immediately posterior to the graft. Crest cells also populated the brachial plexus in accord with their segmental origins. In the forelimb, neural crest cells populated muscle nerves with anterior brachial spinal segments populating nerves to anterior musculature of the forelimb and posterior brachial spinal segments populating nerves to posterior musculature. Similar patterns were seen following both homotopic and heterotopic transplantation. In both types of grafts, the distribution of neural crest cells largely matched the sensory and motor projection pattern from the same spinal segmental level. This suggests that neural crest-derived Schwann cells from a particular spinal segment may use sensory and motor fibers emerging from the same segmental level as substrates to guide their migration into the periphery.
Carpenter
E M
EM
Committee on Neurobiology, University of Chicago, Illinois 60637.
Hollyday
M
M
D000818:Animals; D002642:Chick Embryo; D002678:Chimera; D003370:Coturnix / Q000196:embryology*; D005552:Forelimb / Q000196:embryology* / Q000294:innervation; D009024:Morphogenesis; D009432:Neural Crest / Q000196:embryology; D010525:Peripheral Nerves / Q000166:cytology / Q000196:embryology*; D012583:Schwann Cells / Q000166:cytology*; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology