Scatter factor is a glycoprotein but glycosylation is not required for its activity.
Scatter factor (SF) is a protein produced by fibroblasts, smooth muscle cells, and human placenta which scatter cohesive epithelial cell colonies and increases cellular motility. SF bound to concanavalin A and other lectins with high affinity. SF could also be stained with a glycoprotein specific stain. Incubation of producer cells (N-ras-transformed 3T3), with tunicamycin homolog A1 did not have any significant effect on the secretory activity of SF. The treatment of SF with N- and O-glycanases as well as endoglycosidase H had no effect on its activity. However, treatment of target (Madin Darby canine kidney) cells with tunicamycin A1, abolished the scattering response. These studies suggest that scatter factor is a glycoprotein, but glycosylation is not required for its secretion or activity by the producer cells; however, glycosylation of proteins in the target cells is required for SF action.
Hofmann
R
R
Department of Radiation Oncology, Long Island Jewish Medical Center, New Hyde Park, NY 11042.
Joseph
A
A
Bhargava
M M
MM
Rosen
E M
EM
Goldberg
I
I
D016475:3T3 Cells; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D016207:Cytokines / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D004285:Dogs; D005347:Fibroblasts; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D006031:Glycosylation; D006133:Growth Substances / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D017228:Hepatocyte Growth Factor; D006801:Humans; D007668:Kidney; D037102:Lectins / Q000737:chemistry; D008114:Liver Neoplasms, Experimental / Q000737:chemistry / Q000378:metabolism; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000494:pharmacology; D008715:Methionine / Q000378:metabolism; D051379:Mice; D011257:Pregnancy Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D012685:Sepharose / Q000031:analogs & derivatives / Q000737:chemistry; D014407:Tumor Cells, Cultured; D014415:Tunicamycin / Q000494:pharmacology
D016207:Cytokines; D006023:Glycoproteins; D006133:Growth Substances; D037102:Lectins; D011257:Pregnancy Proteins; C028326:concanavalin A-sepharose; D014415:Tunicamycin; D017228:Hepatocyte Growth Factor; D012685:Sepharose; D008715:Methionine; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase