Effect of combined deficiency of fat and vitamin A on N-nitrosodiethylamine-induced lung carcinogenesis in mice.
The present investigation shows the effect of combined dietary deprivation of fat and vitamin A on N-nitrosodiethylamine (NDEA) induced lung carcinogenesis in male Swiss NMRI mice. Lung tumors were induced by three intragastric administrations of NDEA (80, 80, 40, mg/kg body wt.) in normal saline with a gap of 4 weeks between each administration. The tumor incidence and tumor burden in control animals were found to be 39.1 and 3.9%, respectively. Fat deficiency decreased the tumor incidence to 30.4% and tumor burden to 3.7%. On the other hand, vitamin A deficiency increased them to 95.6 and 6.8%, respectively. Fat deprivation in the diet of animals fed with vitamin A deficient diet decreased the tumor burden and tumor incidence to 59.0 and 4.0%, respectively which were still more than in the control animals. This study suggests a protective role for low intake of fat in vitamin A deficiency.
Khanduja
K L
KL
Department of Biophysics, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Koul
I B
IB
Gandhi
R K
RK
Sehgal
S
S
Sharma
R R
RR
D000818:Animals; D004041:Dietary Fats / Q000378:metabolism*; D004052:Diethylnitrosamine; D008175:Lung Neoplasms / Q000139:chemically induced*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D014801:Vitamin A / Q000378:metabolism; D014802:Vitamin A Deficiency / Q000150:complications*
D004041:Dietary Fats; D014801:Vitamin A; D004052:Diethylnitrosamine