Identification of a human cDNA with high homology to yeast omnipotent suppressor 45.
Omnipotent suppression is a well-established phenomenon in yeast and bacteria in which nonsense mutations are misread. Wild-type (wt) suppressors are presumed to be involved in ensuring the fidelity of translation. We report a human homolog to wt yeast omnipotent suppressor 45 which shares 63% identity at the nucleotide level in the area of open reading frame (ORF) and 73% similarity at the amino acid (aa) level. The aa sequence of the human protein was deduced from a 2.3-kb cDNA (TB3-1) isolated from an adenocarcinoma T84 cell line cDNA library. The cDNA contains an ORF of 1284 bp which encodes a 47.8-kDa protein. Two transcripts for the clone were identified (2.6 and 4.0 kb) in a variety of human cell types. The strong structural similarity to yeast omnipotent suppressor 45, and its widespread expression suggest that this cDNA may play a role in the accurate recognition of nonsense codons in mammalian cells.
Grenett
H E
HE
Department of Cell Biology, University of Alabama, Birmingham 35294.
Bounelis
P
P
Fuller
G M
GM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003062:Codon / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis / Q000235:genetics*; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013489:Suppression, Genetic; D014407:Tumor Cells, Cultured
D003062:Codon; D004274:DNA, Recombinant; D005656:Fungal Proteins; D011506:Proteins; D012269:Ribosomal Proteins