title: Osteoblasts on hydroxyapatite, alumina and bone surfaces in vitro: morphology during the first 2 h of attachment.
abstract: The morphological responses of individual osteoblasts as they attached and spread on hydroxyapatite, bovine bone, alumina with rough and polished surfaces, and tissue culture polystyrene in vitro were examined with scanning electron microscopy. Depending on the surface tested two different morphological sequences were observed during 2 h of adhesion. On alumina, both rough and smooth, bone, and tissue culture polystyrene the cells were round after 0.5 h, and spread radially during the next 1.5 h until they were almost flat, with a nuclear bulge. On hydroxyapatite, however, the cells were flat and circular at 0.5 h, and the edge of the cytoplasm was hardly discernable. This morphology did not change much during the subsequent 1.5 h. The observed cellular morphological response may be related to the bioreactivity of hydroxyapatite.
authors.affiliation: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590.
authors.forename: M A
authors.initials: MA
authors.lastname: Malik
authors.forename: D A
authors.initials: DA
authors.lastname: Puleo
authors.forename: R
authors.initials: R
authors.lastname: Bizios
authors.forename: R H
authors.initials: RH
authors.lastname: Doremus
mesh_terms: D000537:Aluminum Oxide; D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones; D002478:Cells, Cultured; D046508:Culture Techniques; D006882:Hydroxyapatites; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000648:ultrastructure*; D051381:Rats; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites; D000537:Aluminum Oxide