Identification of 2 alpha-hydroxy-4-pregnene-3,20-dione in human pregnancy urine.
2 alpha-Hydroxyprogesterone (2 alpha-hydroxy-4-pregnene-3,20-dione) was identified in human late pregnancy urine by liquid-gel chromatography, GLC and GC-MS. In addition, the following 2-hydroxylated C21 steroids were found and identified as 2 zeta-hydroxy-5 zeta-pregnane-3,20-dione, 2 zeta,20 zeta-dihydroxy-4-pregnen-3-one, 2 alpha,3 alpha-dihydroxy-5 alpha- (and 5 beta)-pregnan-20-one, two isomers of pregnane-2,3,20-triol and 2 zeta,3 zeta,16 zeta-trihydroxy-5 zeta-pregnan-20-one.
Colombier
M
M
Laboratoire de Biochimie Médicale, Faculté de Médecine, Université d'Auvergne, (Clermont I), France.
Gachancard-Bouya
J L
JL
Bègue
R J
RJ
Prost
M
M
D002850:Chromatography, Gel; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006908:Hydroxyprogesterones / Q000652:urine*; D011247:Pregnancy
D006908:Hydroxyprogesterones