Lack of effect of gallium nitrate on bone density in a rat model of simulated microgravity.
The effect of gallium (Ga) nitrate upon bone density was studied in 24 male Sprague-Dawley rats (275-335 g) in an established model of simulated microgravity. Rats, tail-suspended for 14 d from a system of double pulleys, were allowed free mobility with their hind limbs unloaded. Animals were randomized into four groups of six: 1) unsuspended, saline; 2) suspended, saline; 3) unsuspended, Ga; and 4) suspended, Ga. On day 0, 1 d prior to suspension, rats received equal volumes of a single subcutaneous injection of either saline or 30 mg/kg of elemental Ga in the form of Ga nitrate. On day 15, all animals were euthanized under anesthesia, and their hind limbs analyzed in vitro using single-photon absorptiometry. Although we previously demonstrated that Ga inhibits bone resorption in patients with Paget's disease of bone, and although Ga exhibits many apparent similarities in efficacy to diphosphonates, which prevent bone loss in ovariectomized, paralyzed, and single-limb immobilized rats, Ga failed to prevent bone loss in the tail-suspended rats.
Apseloff
G
G
Department of Pharmacology, College of Medicine, Ohio State University, Columbus 43210.
Girten
B
B
Gerber
N
N
Shepard
D R
DR
Matkovic
V
V
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology; D015519:Bone Density / Q000187:drug effects*; D001862:Bone Resorption / Q000188:drug therapy; D005708:Gallium / Q000008:administration & dosage / Q000494:pharmacology*; D006112:Gravitation; D007279:Injections, Subcutaneous; D008297:Male; D010006:Osteoblasts / Q000187:drug effects; D010010:Osteoclasts / Q000187:drug effects; D011187:Posture; D051381:Rats; D011919:Rats, Inbred Strains
D000970:Antineoplastic Agents; D005708:Gallium; C027235:gallium nitrate