title: Purification of the murine heat-stable antigen from erythrocytes.
abstract: The rat anti-mouse erythrocyte (MRBC) monoclonal antibody (mAb), R13, has been developed. The MRBC membrane protein recognized by R13 (R13-Ag) can be purified by loading the butanol-extracted MRBC membrane solution on a R13-conjugated Cellulofine column in the presence of 0.1% CHAPS followed by elution with 1% CHAPS. The amino acid sequence of the affinity-purified R13-Ag corresponded to that predicted from the cDNA for the murine heat-stable antigen. It was revealed that the actual heat-stable antigen was composed of 27 amino acids.
authors.affiliation: Department of Clinical Laboratory Medicine, Ehime University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hitsumoto
authors.forename: A
authors.initials: A
authors.lastname: Nakano
authors.forename: H
authors.initials: H
authors.lastname: Ohnishi
authors.forename: F
authors.initials: F
authors.lastname: Hamada
authors.forename: S
authors.initials: S
authors.lastname: Saheki
authors.forename: N
authors.initials: N
authors.lastname: Takeuchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D051927:CD24 Antigen; D002793:Cholic Acids; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000276:immunology*; D006031:Glycosylation; D008562:Membrane Glycoproteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D051927:CD24 Antigen; C497890:Cd24a protein, mouse; D002793:Cholic Acids; D008562:Membrane Glycoproteins; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate