Cloning and characterization of the gene encoding the ADP-ribosylation factor in Candida albicans.
We have cloned and sequenced the gene (ARF) encoding the ADP-ribosylation factor (ARF) of Candida albicans. The gene contains an open reading frame of 537 nucleotides (nt) that codes for a protein with an Mr of 20,259. The C. albicans ARF gene is 67-70% identical at the nt level to other ARF sequences including those of humans; the deduced amino acid sequence of C. albicans ARF shows a 78-83% identity and 89-92% similarity to the other ARFs. Southern analysis of C. albicans genomic DNA suggested the presence of a second ARF gene. The presence of multiple ARF genes is a consistent finding among the other organisms previously shown to have ARFs.
Denich
K T
KT
Division of Endocrinology, Stanford University School of Medicine, CA 94305.
Malloy
P J
PJ
Feldman
D
D
D020727:ADP-Ribosylation Factors; D000262:Adenylyl Cyclases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D002417:Cattle; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000235:genetics*; D005800:Genes, Fungal; D006801:Humans; D008969:Molecular Sequence Data
D002352:Carrier Proteins; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors; D000262:Adenylyl Cyclases