title: Effect of spaceflight on the extracellular matrix of skeletal muscle after a crush injury.
abstract: The organization and composition of the extracellular matrix were studied in the crush-injured gastrocnemius muscle of rats subjected to 0 G. After 14 days of flight on COSMOS 2044, the gastrocnemius muscle was removed and evaluated by histochemical and immunohistochemical techniques from the five injured flight rodents and various Earth-based treatment groups. In general, the repair process was similar in all injured muscle samples with regard to the organization of the extracellular matrix and myofibers. Small and large myofibers were present within an expanded extracellular matrix, indicative of myogenesis and muscle regeneration. In the tail-suspended animals, a more complete repair was observed with no enlarged area of nonmuscle cells or matrix material visible. In contrast, the muscle samples from the flight animals were less well organized and contained more macrophages and blood vessels in the repair region, indicative of a delayed repair process, but did not demonstrate any chronic inflammation. Myofiber repair did vary in muscles from the different groups, being slowest in the flight animals and most complete in the tail-suspended ones.
authors.affiliation: Department of Physiology, West Virginia University, Morgantown 26506.
authors.forename: W T
authors.initials: WT
authors.lastname: Stauber
authors.forename: V K
authors.initials: VK
authors.lastname: Fritz
authors.forename: T E
authors.initials: TE
authors.lastname: Burkovskaya
authors.forename: E I
authors.initials: EI
authors.lastname: Ilyina-Kakueva
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002196:Capillaries / Q000648:ultrastructure; D002199:Capillary Permeability; D003208:Concanavalin A / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D005109:Extracellular Matrix / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000648:ultrastructure; D009132:Muscles / Q000293:injuries / Q000473:pathology*; D011509:Proteoglycans / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013026:Space Flight
chemical_list: D000911:Antibodies, Monoclonal; D011509:Proteoglycans; D003208:Concanavalin A; D010450:Endopeptidases