Activation of human eosinophil and neutrophil functions by haematopoietic growth factors: comparisons of IL-1, IL-3, IL-5 and GM-CSF.
We compared the effect of haematopoietic growth factors granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-1, IL-3, and IL-5 on the functional activation of human eosinophils and neutrophils from the same donor. All four colony-stimulating factors (CSF) enhanced the phagocytosis of Candida albicans by eosinophils and increased staphylococcal, but not Candida, killing. GM-CSF and IL-5 had a profound stimulating effect on eosinophil staphylocidal activity. GM-CSF and IL-3 enhanced the generation of leukotriene C4 (LTC4) induced by calcium ionophore A23187 and the release of arylsulphatase and beta-glucuronidase from specific and small granules of eosinophils. In contrast, IL-1 and IL-5 had no effect on degranulation. GM-CSF and IL-1 enhanced phagocytosis of C. albicans by neutrophils, and GM-CSF stimulated degranulation and the release of the enzymes beta-glucuronidase and arylsulphatase from neutrophils while IL-1 stimulated the release of arylsulphatase only. This study indicates that the eosinophil-active colony-stimulating factors can markedly enhance the host defence function of the eosinophil and even make it the equal of the neutrophil in staphylocidal activity. The CSF-activated eosinophil, however, may cause inappropriate inflammation and normal tissue damage.
Fabian
I
I
Department of Histology and Cell Biology, Sackler School of Medicine, Tel-Aviv University, Ramat-Aviv, Israel.
Kletter
Y
Y
Mor
S
S
Geller-Bernstein
C
C
Ben-Yaakov
M
M
Volovitz
B
B
Golde
D W
DW
D000818:Animals; D002176:Candida albicans; D006224:Cricetinae; D004804:Eosinophils / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology*; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology; D007377:Interleukin-3 / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D007378:Interleukins / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013211:Staphylococcus aureus
D007375:Interleukin-1; D007377:Interleukin-3; D015848:Interleukin-5; D007378:Interleukins; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor