Nuclear magnetic resonance spectroscopy to determine the micellar cholesterol in human bile.
The cholesterol of gallstones comes from the vesicular rather than the micellar phase of bile. Progress in this field has been limited because conventional analytical methods disturb the distribution of cholesterol between the two phases. The resonance of the cholesterol C6 proton occurs at a chemical shift of 5.4 ppm, to be shown by 2D NMR to be specific for biliary cholesterol, and arises only from the micellar mode. Thus integration of the C6 proton resonance peak area provides a direct non-invasive determination of the cholesterol distribution in human bile.
Ellul
J P
JP
Gastroenterology Unit, United Medical School of Guy's Hospital, London, UK.
Murphy
G M
GM
Parkes
H G
HG
Slapa
R Z
RZ
Dowling
R H
RH
D001646:Bile / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles
D008823:Micelles; D002784:Cholesterol