Measurement of rat plasma adenosine levels during normoxia and hypoxia.
A stop solution containing EDTA, EGTA, dipyridamole, erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA) and d,l-alpha-glycerophosphate has been used to prevent adenosine formation and loss from rat femoral arterial blood samples prepared for measurement of plasma adenosine levels. The femoral arterial plasma adenosine concentration in normoxic rats was 79.2 +/- 12.7 nM. During a 5 min period of hypoxia (8% oxygen) plasma adenosine increased to 190.2 +/- 32.2 nM. A resting plasma adenosine level of circa 80 nM, which is 10X lower than most previous estimates, approximates the threshold levels of adenosine required for arterial dilation.
Phillis
J W
JW
Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201.
O'Regan
M H
MH
Perkins
L M
LM
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000241:Adenosine / Q000097:blood*; D000249:Adenosine Monophosphate / Q000097:blood; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002245:Carbon Dioxide / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004176:Dipyridamole / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000097:blood*; D008297:Male; D010100:Oxygen / Q000097:blood*; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains
D002245:Carbon Dioxide; D000249:Adenosine Monophosphate; D004533:Egtazic Acid; C010192:9-(2-hydroxy-3-nonyl)adenine; D004176:Dipyridamole; D004492:Edetic Acid; D000225:Adenine; D000241:Adenosine; D010100:Oxygen