Induction of phosphatidylinositol-glycan-linked Fc gamma RIII in human monocytic THP-1 cells by transforming growth factor-beta 1 and retinoic acid.
Human monocytic leukemic cell line THP-1 was incubated with transforming growth factor-beta 1 (TGF-beta 1) and retinoic acid (RA) and the expression of Fc gamma RIII was investigated. Fc gamma RIII was induced after incubation of the cells with both TGF-beta 1 and RA but not with either TGF-beta 1 or RA alone. Such effects of TGF-beta 1 and RA were not detected on human promyelocytic HL-60 cells. Northern blot analysis revealed the induction of Fc gamma RIII transcripts in THP-1 cells. Furthermore, the Fc gamma RIIIs newly expressed on the cell surface were cleaved by phosphatidylinositol-specific phospholipase C (PI-PLC) and reacted with monoclonal antibody MG38 which specifically binds to granulocyte-type Fc gamma receptors. These results indicated that TGF-beta 1 could induce phosphatidylinositol-glycan-linked Fc gamma RIII (Fc gamma RIII-I) in THP-1 cells in the presence of RA.
Morikawa
M
M
Tokyo Institute for Immunopharmacology, Inc., Japan.
Harada
N
N
Zeng
H
H
Koike
T
T
Yoshida
T
T
D000943:Antigens, Differentiation / Q000096:biosynthesis*; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D017261:Glycosylphosphatidylinositols; D006801:Humans; D007074:Immunoglobulin G; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D010716:Phosphatidylinositols / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D011961:Receptors, Fc / Q000096:biosynthesis*; D017452:Receptors, IgG; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology
D000943:Antigens, Differentiation; D017261:Glycosylphosphatidylinositols; D007074:Immunoglobulin G; D010716:Phosphatidylinositols; D011134:Polysaccharides; D011961:Receptors, Fc; D017452:Receptors, IgG; D016212:Transforming Growth Factor beta; D014212:Tretinoin; D002117:Calcitriol