title: Relative amounts of 1-O-alkyl- and 1-acyl-2-acetyl-sn-glycero-3-phosphocholine in stimulated endothelial cells.
abstract: The specific precursor for platelet-activating factor, 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine, constitutes 10 per cent of the 1-radyl-2-acyl-sn-glycero-3-phosphocholines in endothelial cells. Stimulation of endothelial cells results in accumulation of PAF and its sn-1-acyl- analog (acylPAF), with acylPAF the predominant product. Mass spectrometry confirmed these relative amounts and confirmed that stimulated endothelial cells accumulate 1-3 ng PAF per million cells. These data suggest that stimulated endothelial cells accumulate both PAF and acylPAF and that the PAF synthetic pathway in endothelial cells is not highly selective for the specific PAF precursor (1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine).
authors.affiliation: Veterans Affairs Medical Center, Salt Lake City, Utah.
authors.forename: R E
authors.initials: RE
authors.lastname: Whatley
authors.forename: K L
authors.initials: KL
authors.lastname: Clay
authors.forename: F H
authors.initials: FH
authors.lastname: Chilton
authors.forename: M
authors.initials: M
authors.lastname: Triggiani
authors.forename: G A
authors.initials: GA
authors.lastname: Zimmerman
authors.forename: T M
authors.initials: TM
authors.lastname: McIntyre
authors.forename: S M
authors.initials: SM
authors.lastname: Prescott
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000032:analysis; D011651:Pulmonary Artery / Q000378:metabolism; D014316:Tritium; D014471:Umbilical Veins / Q000378:metabolism
chemical_list: C036761:1-alkyl-2-acyl-sn-glycero-3-phosphocholine; D010972:Platelet Activating Factor; D014316:Tritium