The role of the exocyclic amino groups of conserved purines in hammerhead ribozyme cleavage.
A series of 2 stranded hammerhead ribozymes has been synthesized in which single conserved adenosine residues have been replaced by nebularine or single guanosine residues by inosine. Comparison of the rates of trans-cleavage for the modified structures suggests the presence of interstrand non-Watson-Crick hydrogen bonding interactions including a GA:AG double mismatch. The exocyclic amino group of one of the guanosine residues is essential for cleavage.
Slim
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MRC Laboratory of Molecular Biology, Cambridge, UK.
Gait
M J
MJ
D000241:Adenosine / Q000737:chemistry; D000588:Amines / Q000737:chemistry*; D001483:Base Sequence; D006151:Guanosine / Q000737:chemistry; D007288:Inosine / Q000737:chemistry; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011684:Purine Nucleosides / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D012263:Ribonucleosides / Q000737:chemistry; D013379:Substrate Specificity
D000588:Amines; D011684:Purine Nucleosides; D016337:RNA, Catalytic; D012263:Ribonucleosides; D006151:Guanosine; D007288:Inosine; C005248:nebularine; D000241:Adenosine