title: An automated method for the analysis of trabecular bone structure.
abstract: Trabecular structure as well as bone mass is important in studies of bone disease and fracture. An automated method for the direct analysis of two-dimensional trabecular micro-anatomy and its application to human iliac crest bone biopsies is described. Compared with established methods which require expensive equipment and complex software, costs have been reduced and availability increased by using an image analyzer driven by a microcomputer. Routine histological sections are accepted and an editing function enables the removal of artifacts. An elastic window allows field expansion for large specimens. The program enables the rapid assessment of the bone volume and trabecular surface from the intact image, followed by image skeletonization and the deduction of the trabecular length, number, character, and spacing together with the number of trabecular junctions and discontinuities; the trabecular width is calculated indirectly. Images may be stored to disk or printed as permanent records for diagnostic or research purposes.
authors.affiliation: Department of Anatomy, University of Leeds, England.
authors.forename: J E
authors.initials: JE
authors.lastname: Aaron
authors.forename: D R
authors.initials: DR
authors.lastname: Johnson
authors.forename: J A
authors.initials: JA
authors.lastname: Kanis
authors.forename: B A
authors.initials: BA
authors.lastname: Oakley
authors.forename: P
authors.initials: P
authors.lastname: O'Higgins
authors.forename: S K
authors.initials: SK
authors.lastname: Paxton
mesh_terms: D001706:Biopsy; D001847:Bone Diseases / Q000473:pathology*; D001857:Bone Matrix / Q000033:anatomy & histology* / Q000473:pathology; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D010001:Osteitis Deformans / Q000473:pathology; D010024:Osteoporosis / Q000473:pathology