Free radical activity during development of insulin-dependent diabetes mellitus in the rat.
Free radical-induced lipid peroxidation was quantified by measuring expired pentane from diabetic prone BB Wistar rats of 45-90 d of age. Insulin-dependent diabetes mellitus was manifest at the age of 71 +/- 8 d. Expired pentane increased from 2.1 +/- 0.7 to 5.0 +/- 3.0 pmol/100g/min (p less than 0.01) at manifestation of the disease and remained high throughout the test period. In healthy age-matched control rats it persisted low. In rats made diabetic with streptozotocin, expired pentane remained low. The changes in expired pentane suggest that the development of endogenous insulin-dependent diabetes mellitus in BB rats is associated with increased free radical activity. This is not due to hyperglycemia or ketosis per se, and reflects a fundamental difference in the free radical activity between the spontaneously diabetic BB rats and the disease produced by streptozotocin. Development of spontaneous insulin-dependent diabetes in BB rats is associated with increased free radical activity that persists after the manifestation of the disease.
Pitkänen
O M
OM
Children's Hospital, I Department of Pediatrics, Helsinki, Finland.
Martin
J M
JM
Hallman
M
M
Akerblom
H K
HK
Sariola
H
H
Andersson
S M
SM
D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D005609:Free Radicals / Q000737:chemistry; D008054:Lipid Peroxides / Q000737:chemistry / Q000378:metabolism*; D010420:Pentanes / Q000378:metabolism; D051381:Rats; D011913:Rats, Inbred BB; D011919:Rats, Inbred Strains; D013311:Streptozocin
D005609:Free Radicals; D008054:Lipid Peroxides; D010420:Pentanes; C033353:pentane; D013311:Streptozocin