Production of monoclonal antibodies against a novel glycoprotein synthesized and secreted by dog thyroid C-cells.
A monoclonal antibody (MAb) that reacted only with thyroid C-cells was raised against cell suspensions from dog thyroid glands, to examine a glycoprotein secreted by C-cells. After chronically-induced hypercalcemia and administration of an anti-thyroid drug, reaction products for the antibody markedly decreased in C-cells, coinciding with alterations in calcitonin immunoreactivity. The antigen recognized by the MAb appears to be a secretory protein. The MAb reacted with C-cells from a wide variety of mammalian species, including rats, mice, hamsters, cattle, cats, rabbits, and monkeys. Furthermore, tumor cells of human medullary thyroid carcinoma, which is derived from C-cells, were immunoreactive to the MAb. Exceptionally, C-cells from guinea pigs and pigs were not stained with the MAb. No crossreactivity was observed in any of the dog tissues examined. Immunoblot analysis demonstrated that the MAb recognized a single prominent band at a molecular weight of approximately 79,000. The 79 KD band reacted with various digoxigenin-labeled lectins, including GNA, DSA, SNA, and MAA; it is a glycoprotein containing mannose, N-acetylglucosamine, and sialic acid. Dog thyroid C-cells were also densely stained with these lectins. The results indicate that thyroid C-cells synthesize and secrete a specific glycoprotein in addition to peptide hormones.
Kameda
Y
Y
Department of Anatomy, Kitasato University School of Medicine, Kanagawa, Japan.
D000117:Acetylglucosamine / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured / Q000378:metabolism; D003429:Cross Reactions; D003594:Cytoplasmic Granules / Q000378:metabolism; D004076:Digoxigenin; D004285:Dogs; D006023:Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism; D006825:Hybridomas; D006934:Hypercalcemia / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D037102:Lectins; D008358:Mannose / Q000032:analysis*; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000032:analysis*; D013961:Thyroid Gland / Q000378:metabolism* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D037102:Lectins; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid; D004076:Digoxigenin; D008358:Mannose; D000117:Acetylglucosamine