Xlcaax-1 is localized to the basolateral membrane of kidney tubule and other polarized epithelia during Xenopus development.
Xlcaax-1 is a novel, maternally expressed, 110-kDa, CAAX box containing protein that undergoes isoprenylation and palmitoylation through which it associates with the plasma membrane. We report here the cellular and subcellular localization of the xlcaax-1 protein during development of Xenopus laevis. Whole-mount immunocytochemistry and immunoperoxidase staining of tissue sections show that during development the xlcaax-1 protein accumulation is coincident with the differentiation of the epidermis, pronephros, and mesonephros. In the pronephros and mesonephros the xlcaax-1 protein is localized to the basolateral membrane of differentiated tubule epithelial cells. Thus, the xlcaax-1 protein serves as a marker for tubule formation and polarization during Xenopus kidney development. Xlcaax-1 may also be used as a marker for the functional differentiation of the epidermis and the epidermally derived portions of the lens and some cranial nerves. Western blot analysis shows that in the adult the xlcaax-1 protein is most abundant in kidney. Immunogold EM analysis shows that the xlcaax-1 protein is highly enriched in the basal infoldings of the basolateral membrane of the epithelial cells in adult kidney distal tubules. In addition, immunoperoxidase staining of tissue sections detected low levels of xlcaax-1 protein in the epithelial cells of skin, urinary bladder, gall bladder, and parietal glands of the stomach. The localization pattern of xlcaax-1 suggests that the protein may function in association with an ion transport channel or pump.
Cornish
J A
JA
Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Kloc
M
M
Decker
G L
GL
Reddy
B A
BA
Etkin
L D
LD
D000818:Animals; D002454:Cell Differentiation; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D004848:Epithelium / Q000196:embryology; D007684:Kidney Tubules / Q000196:embryology / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis*; D008650:Mesonephros / Q000196:embryology / Q000648:ultrastructure; D012867:Skin / Q000196:embryology; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology*
D008565:Membrane Proteins; C068700:PALM3 protein, Xenopus; D029867:Xenopus Proteins