Neuron-specific antigen HPC-1 from bovine brain reveals strong homology to epimorphin, an essential factor involved in epithelial morphogenesis: identification of a novel protein family.
We have already cloned the cDNA for the HPC-1 antigen, a neuron-specific protein antigen from the rat brain. Here we report the molecular cloning of the bovine HPC-1 antigen homologue, and much strong sequence conservation between rat and bovine. By searching the recent protein data base, it was found that the HPC-1 antigen revealed unusual similarity to epimorphin which was mesenchymal factor related to the morphogenesis of primitive epidermal tissues in embryonic stages. We also found that the HPC-1 antigen was identical to p35A (syntaxin) which bound both to a synaptic vesicle protein and to N-type calcium channel. Although the relationship of the physiological functions, structures and topologies along cellular membrane between the HPC-1 antigen and epimorphin have not been consistent yet, these two proteins belong to a novel protein family.
Inoue
A
A
Department of Physiological Sciences, School of Life Sciences, Graduate University for Advanced Studies, Aichi, Japan.
Akagawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface; D001483:Base Sequence; D001923:Brain Chemistry; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004848:Epithelium / Q000502:physiology; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000502:physiology; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018377:Neurotransmitter Agents; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D050827:Syntaxin 1
D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D012333:RNA, Messenger; C494212:STX1A protein, human; C494213:Stx1a protein, rat; C504581:Stx2 protein, rat; D050827:Syntaxin 1; C076849:STX1A protein, Bos taurus; D004247:DNA