Inhibition of trypsin by the beta-amyloid protein precursor. A comparative study between transfected cells, human brain and cerebrospinal fluid.
Soluble beta-amyloid protein precursors (beta-APPs) were studied in human brain and cerebrospinal fluid (CSF) after partial purification by ion exchange chromatography. Proteins were analysed in immunoblotting experiments using a monoclonal antibody directed against the N-terminal segment of the beta-APP 770, and by reverse enzymography. In the human brain and CSF, a protein which comigrates with the beta-APP 770 expressed by transfected CHO cells was able to inhibit trypsin.
Delvaux
A
A
Laboratoire de Neurochimie, Université Catholique de Louvain, Brussels, Belgium.
Van der Elst
L
L
Octave
J N
JN
D016564:Amyloid beta-Protein Precursor / Q000134:cerebrospinal fluid / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D016466:CHO Cells; D002460:Cell Line; D002848:Chromatography, DEAE-Cellulose; D006224:Cricetinae; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D014162:Transfection; D014361:Trypsin Inhibitors
D016564:Amyloid beta-Protein Precursor; D003470:Culture Media; D014361:Trypsin Inhibitors