title: Yellow fever 17DD vaccine virus is temperature sensitive when grown in mosquito C6-36 cells.
abstract: Twenty-three yellow fever (YF) vaccine viruses and three wild-type YF viruses were propagated independently in human adenocarcinoma (SW13) cells and mosquito Aedes albopictus C6-36 cells. The three YF 17DD vaccine viruses were found to be slightly temperature sensitive (ts) at 39.5 degrees C versus 37.0 degrees C (efficiency of plaquing 0.04 to 0.1) following propagation in C6-36 but not in SW13 cells. A plaque-purified preparation of the 17DD vaccine manufactured in Brazil was ts when grown in C6-36 cells and remained ts when passaged back into the SW13 cell line.
authors.affiliation: Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, UK.
authors.forename: T N
authors.initials: TN
authors.lastname: Ledger
authors.forename: B K
authors.initials: BK
authors.lastname: Sil
authors.forename: L M
authors.initials: LM
authors.lastname: Dunster
authors.forename: J R
authors.initials: JR
authors.lastname: Stephenson
authors.forename: P D
authors.initials: PD
authors.lastname: Minor
authors.forename: A D
authors.initials: AD
authors.lastname: Barrett
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D002460:Cell Line; D006358:Hot Temperature; D006801:Humans; D010641:Phenotype; D014407:Tumor Cells, Cultured; D014765:Viral Vaccines / Q000032:analysis*; D015005:Yellow fever virus / Q000235:genetics / Q000254:growth & development*
chemical_list: D014765:Viral Vaccines