Identification of recoverin-like immunoreactivity in mouse brain.
Mouse brain was found by immunoblot analysis to have a protein of Mr 23,000 (P23k) that was clearly different from recoverin and was labeled with an antiserum raised against the NH2-terminus of recoverin. P23k could not be detected by an antiserum raised against the COOH-terminus of recoverin. Blots with 45Ca demonstrated that P23k bound Ca2+ and had a Ca(2+)-dependent membrane-binding property. Immunohistochemical studies provided the specific distribution of P23k in cerebral cortex and cerebellum.
Takamatsu
K
K
Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
Uyemura
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002531:Cerebellum / Q000737:chemistry / Q000166:cytology; D002540:Cerebral Cortex / Q000737:chemistry / Q000166:cytology; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins; D051597:Hippocalcin; D007106:Immune Sera; D015151:Immunoblotting; D007150:Immunohistochemistry; D008074:Lipoproteins; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009419:Nerve Tissue Proteins; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology; D009928:Organ Specificity; D051603:Recoverin; D012160:Retina / Q000737:chemistry / Q000166:cytology*
D002135:Calcium-Binding Proteins; D005136:Eye Proteins; C496692:Hpca protein, mouse; D007106:Immune Sera; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D009842:Oligopeptides; C496732:Rcvrn protein, mouse; D051603:Recoverin; D051597:Hippocalcin