Coexistence of two calsequestrin isoforms in rabbit slow-twitch skeletal muscle fibers.
The cardiac and skeletal muscle isoforms of calsequestrin (CS), the low affinity, high capacity Ca2+ binding protein localized in the lumen of sarcoplasmic reticulum, are the products of two different genes (Fliegel, L., Leberer, E., Green, N.M. and MacLennan, D.H. (1982) FEBS Lett. 242, 297-300), and can be both purified from slow-twitch skeletal muscle of the rabbit (Damiani, E., Volpe, P. and Margreth, A. (1990) J. Muscle Res. Cell Motil. 11, 522-530). Here we show that both CS isoforms coexist in slow-twitch muscle fibers as indicated by indirect immunofluorescent staining of cryosections with affinity-purified antibodies specific for each CS isoform.
Biral
D
D
Centro di Studio per la Biologia e la Fisiopatologia Muscolare del CNR, Università di Padova, Italy.
Volpe
P
P
Damiani
E
E
Margreth
A
A
D000818:Animals; D015153:Blotting, Western; D002155:Calsequestrin / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D007536:Isomerism; D051379:Mice; D009132:Muscles / Q000378:metabolism*; D011817:Rabbits
D002155:Calsequestrin