title: Demonstration of Cu-Zn superoxide dismutase in rat liver peroxisomes. Biochemical and immunochemical evidence.
abstract: In this study, by using highly purified rat liver peroxisomes, we provide evidence from analytical cell fractionation, Western blot, and immunocytochemical analysis that Cu-Zn superoxide dismutase is present in animal peroxisomes. Treatment with ciprofibrate, a peroxisome proliferator, increased the peroxisomal superoxide dismutase activity by 3-fold with no effect on mitochondrial activity but a marked decrease in cytosolic superoxide dismutase activity, further supporting that besides cytosolic and mitochondrial localization, Cu-Zn superoxide dismutase is present in peroxisomes also. Demonstration of superoxide dismutase in peroxisomes suggests a new role for this organelle in pathophysiological conditions, such as ischemia-reperfusion injury.
authors.affiliation: Department of Pediatrics and Pathology, Medical University of South Carolina, Charleston 29425.
authors.forename: G S
authors.initials: GS
authors.lastname: Dhaunsi
authors.forename: S
authors.initials: S
authors.lastname: Gulati
authors.forename: A K
authors.initials: AK
authors.lastname: Singh
authors.forename: J K
authors.initials: JK
authors.lastname: Orak
authors.forename: K
authors.initials: K
authors.lastname: Asayama
authors.forename: I
authors.initials: I
authors.lastname: Singh
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002995:Clofibric Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D058607:Fibric Acids; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008830:Microbodies / Q000201:enzymology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D058607:Fibric Acids; D002995:Clofibric Acid; D013482:Superoxide Dismutase; C019304:ciprofibrate