Effect of allogenic Dalton's lymphoma cells on the activation of murine peritoneal macrophages to tumoricidal state by cisplatin and lipopolysaccharide (LPS).
Murine peritoneal macrophages on treatment with cisplatin or LPS for 24 h showed enhanced tumoricidal activity. Co-incubation of macrophages with fresh allogeneic Dalton's lymphoma (DL) cells significantly inhibited the activation of macrophages to tumoricidal state by cisplatin or LPS. Incubation of macrophages with DL cell culture supernatants or paraformaldehyde (PFA) fixed DL cells also significantly down-regulated the activation of macrophages with cisplatin or LPS. On the other hand, preexposure of macrophages to DL cells for 24 h before activation with cisplatin and LPS enhanced the tumoricidal activity of murine peritoneal macrophages. These results may contribute to understanding the effect of tumor cells on macrophages.
Singh
S M
SM
School of Biotechnology, Banaras Hindu University, Varanasi, U.P., India.
Singh
R K
RK
Sodhi
A
A
D000818:Animals; D002945:Cisplatin / Q000494:pharmacology*; D003470:Culture Media; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology; D007111:Immunity, Cellular / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides; D008223:Lymphoma / Q000276:immunology* / Q000473:pathology; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D010529:Peritoneal Cavity / Q000166:cytology; D011108:Polymers / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D003470:Culture Media; D008070:Lipopolysaccharides; D011108:Polymers; D005557:Formaldehyde; D002945:Cisplatin; C003043:paraform