[Effects of magnesium on early post-depolarization and trigger activity induced by cesium on Purkinje fibers in ferrets].
The effects of magnesium (Mg) on afterdepolarisations and cesium-induced activity were studied in 15 preparations of ferret Purkinje fibres. When the perfusion medium was enriched with Mg to concentrations of 5 mM, the duration of the action potentials stopped increasing and the diastolic membrane potential was stabilised despite persistent exposure to cesium. A suppressor effect of Mg on the high and low voltage early afterdepolarisations was observed which was significant at 2.2 mM (p less than 0.03) and even more so at 5 mM (p = 3. 10-5) related to a lower probability of early afterdepolarisations. A suppressor effect of Mg on high and low voltage induced activities was also recorded, significant at 5 mM, due to a reduction in the probability of apparition of a first induced action potential (p = 2. 10-4) and a reduction in the number of induced action potentials (p = 8. 10-4). The rapid onset of action (a few minutes) of Mg suggests an extracellular mode of action. The suppressor effect of Mg is probably related to inhibition of an unmasked current by the cesium, a calcium antagonist effect and/or non-specific membrane stabilising effect. The suppressor effect of Mg on early afterdepolarisations and induced activity could, at least partially, explain its in vito antiarrhythmic action on torsades de pointe.
Borgat
C
C
Service de physiologie et explorations fonctionnelles, hôpital G, Nantes.
Charpentier
F
F
Le Marec
H
H
D000200:Action Potentials / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002586:Cesium / Q000494:pharmacology*; D004594:Electrophysiology; D005289:Ferrets; D008274:Magnesium / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D011690:Purkinje Fibers / Q000187:drug effects* / Q000502:physiology
D002586:Cesium; D008274:Magnesium
Effets du magnésium sur les post-dépolarisations précoces et l'activité déclenchée induites par le césium sur les fibres de Purkinje de furet.