Human intraovarian interleukin-1 (IL-1) system: highly compartmentalized and hormonally dependent regulation of the genes encoding IL-1, its receptor, and its receptor antagonist.
To delineate the scope of the human intraovarian IL-1 system we used a solution hybridization/RNase protection assay to test for expression of the genes encoding IL-1, its type I receptor (IL-1R), and its receptor antagonist (IL-1RA). IL-1 transcripts were not detected in whole ovarian material from days 4 or 12 of an unstimulated menstrual cycle but transcripts (IL-1 beta much greater than IL-11 alpha) were detected in preovulatory follicular aspirates from gonadotropin-stimulated cycles. Concurrently obtained peripheral monocytes did not contain IL-1 beta transcripts but macrophage-depleted follicular aspirates did, thus implicating the granulosa cells as the site of IL-1 expression. IL-1R transcripts were detected in RNA from whole ovaries and follicular aspirates but not in RNA from peripheral monocytes. IL-1RA transcripts were detected in whole ovarian material as well as in macrophage-free follicular aspirates. Cultured human granulosa and theca cells did not contain mRNA for IL-1 beta or IL-1RA but did contain mRNA for IL-1R. Treatment of cell cultures with forskolin (25 microM) induced IL-1 beta transcripts in granulosa but not theca cells. Forskolin also increased the basal levels of IL-1R transcripts in both granulosa and theca cells but did not induce IL-RA transcripts in either cell type. Taken together, these findings reveal the existence of a complete, highly compartmentalized, hormonally dependent intraovarian IL-1 system replete with ligands, receptor, and receptor antagonist.
Hurwitz
A
A
Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.
Loukides
J
J
Ricciarelli
E
E
Botero
L
L
Katz
E
E
McAllister
J M
JM
Garcia
J E
JE
Rohan
R
R
Adashi
E Y
EY
Hernandez
E R
ER
D000328:Adult; D002478:Cells, Cultured; D046508:Culture Techniques; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006062:Gonadotropins / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000235:genetics* / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D017472:Receptors, Interleukin-1
D006062:Gonadotropins; D007375:Interleukin-1; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1