title: Both membrane stretch and fatty acids directly activate large conductance Ca(2+)-activated K+ channels in vascular smooth muscle cells.
abstract: Large conductance Ca(2+)-activated K+ channels in rabbit pulmonary artery smooth muscle cells are activated by membrane stretch and by arachidonic acid and other fatty acids. Activation by stretch appears to occur by a direct effect of stretch on the channel itself or a closely associated component. In excised inside-out patches stretch activation was seen under conditions which precluded possible mechanisms involving cytosolic factors, release of Ca2+ from intracellular stores, or stretch induced transmembrane flux of Ca2+ or other ions potentially capable of activating the channel. Fatty acids also directly activate this channel. Like stretch activation, fatty acid activation occurs in excised inside-out patches in the absence of cytosolic constituents. Moreover, the channel is activated by fatty acids which, unlike arachidonic acid, are not substrates for the cyclo-oxygenase or lypoxygenase pathways, indicating that oxygenated metabolites do not mediate the response. Thus, four distinct types of stimuli (cytosolic Ca2+, membrane potential, membrane stretch, and fatty acids) can directly affect the activity of this channel.
authors.affiliation: Department of Physiology, University of Massachusetts Medical School, Worcester 01655.
authors.forename: M T
authors.initials: MT
authors.lastname: Kirber
authors.forename: R W
authors.initials: RW
authors.lastname: Ordway
authors.forename: L H
authors.initials: LH
authors.lastname: Clapp
authors.forename: J V
authors.initials: JV
authors.lastname: Walsh
authors.forename: J J
authors.initials: JJ
authors.lastname: Singer
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002412:Cations; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D005227:Fatty Acids / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019814:Myristic Acid; D009227:Myristic Acids / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D011651:Pulmonary Artery / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits
chemical_list: D002412:Cations; D005227:Fatty Acids; D009227:Myristic Acids; D015221:Potassium Channels; D019814:Myristic Acid; D016718:Arachidonic Acid; D002118:Calcium