title: Regional and generalized changes in cytosolic free calcium in monocytes during phagocytosis.
abstract: We measured and visualized cytosolic free calcium ([Ca2+]i) in individual human peripheral blood monocytes during phagocytosis by using the fluorescent indicator fura-2. Monocytes exhibit a rapid rise in [Ca2+]i from a basal level of 75 +/- 11 nM to a peak level of 676 +/- 78 nM (means +/- standard errors of the means; P less than 0.001) by 34 +/- 5 s after contact with opsonized zymosan particles, and a thin rim of high [Ca2+]i was observed surrounding the ingested particle.
authors.affiliation: Department of Medicine, University of Virginia School of Medicine, Charlottesville 22908.
authors.forename: E
authors.initials: E
authors.lastname: Kim
authors.forename: R I
authors.initials: RI
authors.lastname: Enelow
authors.forename: G W
authors.initials: GW
authors.lastname: Sullivan
authors.forename: G L
authors.initials: GL
authors.lastname: Mandell
mesh_terms: D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D010587:Phagocytosis
chemical_list: D002118:Calcium