title: Conservation of intergenic spacer length in ribosomal DNA of the tailed frog, Ascaphus truei.
abstract: We have examined the organization of cloned rDNA [encoding ribosomal RNA (rRNA)] repeat units from the tailed frog, Ascaphus truei, and have compared rDNA spacer lengths in the genomes of eleven individuals from two widely-separated populations. This comparison has shown that the A. truei spacer is always very short (about 1.5 kb) and that it is remarkably constant in length. In none of the individuals tested were more than two spacer-length classes found and the maximum difference in spacer length found in comparisons both within single animals and across both populations was about 120 bp. We point out those structural features that may contribute to the unusual stability of this spacer and the consequent absence of the extensive length heterogeneities found amongst rDNA repeat units in most genomes.
authors.affiliation: Department of Genetics, University of Nottingham, Queens Medical Centre, U.K.
authors.forename: G T
authors.initials: GT
authors.lastname: Morgan
authors.forename: K M
authors.initials: KM
authors.lastname: Middleton
mesh_terms: D000818:Animals; D001001:Anura / Q000235:genetics*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S