[Synchronous spectrofluorimetry for simultaneous determination of riboflavin and pyridoxine and its application in multivitamin preparations].
A new method for the simultaneous determination of riboflavin and pyridoxine by synchronous spectrofluorimetry is described. The measurement was carried out in a buffer solution of pH 7 with delta lambda = 58 nm for synchronous scanning. Under the experimental conditions two peaks appeared at 526 nm and 389 nm in the obtained synchronous fluorescence spectrum, corresponding to riboflavin and pyridoxine, respectively. This method was proved to be simple and specific. The fluorescence relative intensity is linearly related with concentration over the range of 0-1 microgram/ml and 0-1.5 micrograms/ml for riboflavin and pyridoxine, respectively. The detection limit was 0.5 ng/ml for riboflavin and 1 ng/ml for pyridoxine. The relative errors of the measurements were less than 5% for artificial samples. The proposed method has been applied to the analyses of riboflavin and pyridoxine in three kinds of commercial multivitamin preparations. The obtained synchronous spectra of multivitamin preparations coincided with those of the mixed standard solution of riboflavin and pyridoxine. Recoveries of 93.5-105.7% were obtained.
Li
Y Q
YQ
Department of Chemistry, Xiamen University.
Huang
X Z
XZ
Xu
J G
JG
Chen
G Z
GZ
D004338:Drug Combinations; D011736:Pyridoxine / Q000032:analysis*; D012256:Riboflavin / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D004338:Drug Combinations; D011736:Pyridoxine; D012256:Riboflavin