Two novel decaglycosylceramides with a blood group A-active tetrasaccharide repeat in the epithelial cells of the small intestine of inbred rats.
A novel type of blood group A-active glycosphingo-lipid was isolated from the epithelial cells of the small intestine of one strain of inbred rats. Electron-impact mass spectrometry of the permethylated and LiAlH4-reduced glycolipid indicated that it is a decaglycosylceramide with a branched oligosaccharide chain. Methylation analysis, gas chromatography-mass spectroscopy of the partially methylated alditol acetates, sequential degradation by exoglycosidases and characterization of the reaction products by TLC immunostaining with appropriate anti-A and anti-H antibodies, and 1H NMR spectrometry resulted in the characterization of a decaglycosylceramide with two variants in a 7/3 ratio. It was termed AA-10. [formula: see text] The major variant has only type 1 chains, whereas the minor one has type 2 chain in the C6-linked branch. This is a novel type of glycolipid with a blood group A-active tetrasaccharide repeat. Genetic analysis demonstrated that AA-10 is inherited as an autosomal dominant trait.
Bouhours
D
D
Institut National de la Santé et de la Recherche Médicale, Unité 76, Paris, France.
Hansson
G C
GC
Angström
J
J
Jovall
P A
PA
Bouhours
J F
JF
D000017:ABO Blood-Group System / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D005963:Glucosylceramides / Q000737:chemistry*; D006028:Glycosphingolipids / Q000737:chemistry*; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000737:chemistry* / Q000166:cytology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D051381:Rats; D011919:Rats, Inbred Strains
D000017:ABO Blood-Group System; D005963:Glucosylceramides; D006028:Glycosphingolipids; D009844:Oligosaccharides