title: The bovine renal epithelial cell line NBL-1 expresses a broad specificity Na(+)-dependent neutral amino acid transport system (System Bo) similar to that in bovine renal brush border membrane vesicles.
abstract: (1) In the bovine renal epithelial cell line NBL-1, transport of alanine and glutamine is highly Na(+)-dependent while the transport of leucine and phenylalanine is also stimulated by Na+ although to a much lesser extent. (2) Na(+)-dependent alanine transport is insensitive to inhibition by methyl AIB, lysine and glutamate but is inhibited by a range of other neutral amino acids. (3) Inhibition of Na(+)-dependent alanine transport by glutamine, phenylalanine and leucine is competitive indicating that these amino acids are transported on a common carrier. (4) Amino acid transport in these cells appears to be localised preferentially on the basolateral membrane. (5) The results are consistent with the presence in confluent cells of a broad-specificity Na(+)-dependent neutral amino acid transport system (System Bo) similar to that in bovine BBMV. An Na(+)-independent system with high activity similar to System L is also present. (6) It is argued that previous results in the literature are consistent with the occurrence of this broad-specificity system in other renal cell lines.
authors.affiliation: Department of Biochemistry, University of Bristol, UK.
authors.forename: F A
authors.initials: FA
authors.lastname: Doyle
authors.forename: J D
authors.initials: JD
authors.lastname: McGivan
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002417:Cattle; D002460:Cell Line; D004848:Epithelium / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008871:Microvilli / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D012964:Sodium