Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads.
Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population containing essentially only tumor cells. Removal of cells from the beads is possible by papain treatment. Negative selection of tumor cells by means of beads coated with a leukocyte binding antibody offers no advantage since dead cells and erythrocytes remain.
Kemmner
W
W
Institut für Biochemie II, Universität Heidelberg, Germany.
Moldenhauer
G
G
Schlag
P
P
Brossmer
R
R
D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D015179:Colorectal Neoplasms / Q000276:immunology / Q000473:pathology*; D004848:Epithelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007158:Immunologic Techniques; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008280:Magnetics; D008863:Microspheres
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface