Photosensitized formation of 8-hydroxydeoxyguanosine in cellular DNA by riboflavin.
Photosensitized formation of 8-hydroxydeoxyguanosine (oh8dG) in DNA by riboflavin has recently been shown in vitro. The present study describes the formation of oh8dG in cellular DNA by photo-irradiation of cultured mammalian cells in the presence of riboflavin. Formation of oh8dG was dependent on the concentration of riboflavin as well as the duration of photoirradiation. These results suggest that photosensitized formation of oh8dG in DNA by riboflavin may be involved in photocarcinogenesis.
Yamamoto
F
F
Biology Division, National Cancer Center Research Institute, Tokyo, Japan.
Nishimura
S
S
Kasai
H
H
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002460:Cell Line; D004247:DNA / Q000378:metabolism / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008027:Light; D008223:Lymphoma / Q000378:metabolism; D051379:Mice; D012256:Riboflavin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; D012256:Riboflavin