An efficient microtest to study adherence of bacteria to mammalian cells.
A simple, fast and highly reproducible microtest was developed for in vitro adherence studies. A rat epithelial cell line was investigated for the adherence of clinical and subclinical ovine and bovine Staphylococcus aureus strains isolated from mastitis. Staphylococcus aureus strains differed in their ability to adhere to epithelial cells, the degree of adherence being dependent on the concentration of bacteria used in the test.
Aguilar
B
B
Department of Animal Production, Servicio de Investigación Agraria (Diputación General de Aragón), Zaragoza, Spain.
Iturralde
M
M
Baselga
R
R
Amorena
B
B
D000818:Animals; D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D002417:Cattle; D002460:Cell Line; D004848:Epithelium / Q000382:microbiology; D007413:Intestinal Mucosa / Q000382:microbiology; D008414:Mastitis, Bovine / Q000382:microbiology; D051381:Rats; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology; D013203:Staphylococcal Infections / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000502:physiology*