title: Preliminary biological characterization of a melanization stimulating factor (MSF) from the dorsal skin of the channel catfish, Ictalurus punctatus.
abstract: A two step fractionation of conditioned media made from the darkly pigmented dorsal skin of the channel catfish, Ictalurus punctatus, has produced fractions that contain a melanization stimulating factor (MSF). Isolated neural tubes of Xenopus laevis embryos exposed to conditioned media and to specific fractions exhibit greater melanization (increased numbers of melanized cells and elevated percentages of melanized cells), a greater number of dendrites per melanized cell, and a greater number of emigrated neural crest cells than control neural tubes. The presence of MSF activity in the darkly pigmented dorsal integument suggests a role for a molecule or molecules in the development and maintenance of the dorsal/ventral pigment pattern of this piscine species and possibly of other vertebrates.
authors.affiliation: Department of Anatomy, University of Arizona, Tucson 85724.
authors.forename: W C
authors.initials: WC
authors.lastname: Johnson
authors.forename: P
authors.initials: P
authors.lastname: Samaraweera
authors.forename: A
authors.initials: A
authors.lastname: Zuasti
authors.forename: J H
authors.initials: JH
authors.lastname: Law
authors.forename: J T
authors.initials: JT
authors.lastname: Bagnara
mesh_terms: D000818:Animals; D006358:Hot Temperature; D007059:Ictaluridae / Q000378:metabolism / Q000502:physiology*; D008543:Melanins / Q000378:metabolism / Q000502:physiology; D009074:Melanocyte-Stimulating Hormones / Q000302:isolation & purification* / Q000494:pharmacology / Q000502:physiology; D008722:Methods; D010858:Pigmentation / Q000187:drug effects / Q000502:physiology*; D012867:Skin / Q000737:chemistry*; D012879:Skin Physiological Phenomena; D014357:Trypsin / Q000494:pharmacology; D014982:Xenopus laevis
chemical_list: D008543:Melanins; D009074:Melanocyte-Stimulating Hormones; D014357:Trypsin