Glutamyl-tRNA reductase activity in Bacillus subtilis is dependent on the hemA gene product.
The Bacillus subtilis hemAXCDBL operon encodes enzymes for the synthesis of 5-aminolaevuline acid via the C5 pathway (hemA and hemL) and uroporphyrinogen III (hemB, hemC and hemD). B. subtilis HemA protein (molecular mass 50 kDa) was overexpressed in hemA mutant of both Escherichia coli and B. subtilis. A mutant B. subtilis HemA protein with a Cys to Tyr change at position 105 was also overexpressed. Both wild-type and mutant HemA proteins migrated as oligomers (molecular mass greater than or equal to 230 kDa) on gel-filtration columns. All column fractions containing wild-type HemA protein had glutamyl-tRNA reductase activity. No glutamyl-tRNA reductase activity was found with the mutant HemA protein. It is concluded that the B. subtilis hemA gene product is identical to, or part of, the glutamyl-tRNA reductase of the C5 pathway.
Schröder
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I
Department of Microbiology, University of Lund, Sweden.
Hederstedt
L
L
Kannangara
C G
CG
Gough
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P
D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000622:Aminolevulinic Acid / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005971:Glutamates / Q000378:metabolism; D008970:Molecular Weight; D009154:Mutation; D010957:Plasmids; D012352:RNA, Transfer, Glu / Q000378:metabolism; D013379:Substrate Specificity; D014169:Transformation, Bacterial
D005971:Glutamates; D012352:RNA, Transfer, Glu; C057794:glutamate-1-semialdehyde; D000622:Aminolevulinic Acid; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase