title: Localization of the human gene for the type I cyclic GMP-dependent protein kinase to chromosome 10.
abstract: We have recently characterized cDNAs and genomic DNA fragments for human type I cGMP-dependent protein kinase (cGK). By probing human x hamster hybrid cell lines with a 1.2-kb intron fragment from the human type I cGK gene, we identified a 5.9-kb BglII restriction fragment and localized it to human chromosome 10. In situ hybridization analyses using 3H-labeled cDNA and genomic DNA probes for the human type I cGK to human metaphase chromosomes supported the somatic cell hybrid data and indicated that the gene (PRKG1B; protein kinase, cGMP-dependent) maps to 10p11.2----q11.2.
authors.affiliation: Institute of Medical Biochemistry, Rikshospitalet, University of Oslo, Norway.
authors.forename: S
authors.initials: S
authors.lastname: Orstavik
authors.forename: M
authors.initials: M
authors.lastname: Sandberg
authors.forename: D
authors.initials: D
authors.lastname: Bérubé
authors.forename: V
authors.initials: V
authors.lastname: Natarajan
authors.forename: J
authors.initials: J
authors.lastname: Simard
authors.forename: U
authors.initials: U
authors.lastname: Walter
authors.forename: R
authors.initials: R
authors.lastname: Gagné
authors.forename: V
authors.initials: V
authors.lastname: Hansson
authors.forename: T
authors.initials: T
authors.lastname: Jahnsen
mesh_terms: D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D011494:Protein Kinases / Q000235:genetics*
chemical_list: D011494:Protein Kinases