Crystalline enzyme kinetics: activity of the Streptomyces R61 D-alanyl-D-alanine peptidase.
The specificity constant, kcat/Km, for the hydrolysis of hippuryl-mercaptoacetate by crystals of the Streptomyces R61 D-D peptidase was measured by reaction of the thiol produced with 4,4'-dithiodipyridine. The values of kcat/Km for the crystal and in solution were the same (within experimental error). A novel method for treating the lag in the progress curves was developed.
Kelly
J A
JA
Sir William Dunn School of Pathology, University of Oxford, U.K.
Waley
S G
SG
Adam
M
M
Frère
J M
JM
D002268:Carboxypeptidases / Q000378:metabolism*; D003460:Crystallization; D004220:Disulfides / Q000378:metabolism; D006626:Hippurates / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D011092:Polyethylene Glycols / Q000378:metabolism; D011725:Pyridines / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*; D013439:Sulfhydryl Reagents / Q000378:metabolism; D013864:Thioglycolates / Q000378:metabolism*
D004220:Disulfides; D006626:Hippurates; D011725:Pyridines; D013439:Sulfhydryl Reagents; D013864:Thioglycolates; C073654:hippuryl-mercaptoacetate; C007542:4,4'-dipyridyl disulfide; D011092:Polyethylene Glycols; D002268:Carboxypeptidases; C033455:zinc D-Ala-D-Ala carboxypeptidase