Crystallization and preliminary analysis of oxidized, recombinant, heterocyst [2Fe-2S] ferredoxin from Anabaena 7120.
The [2Fe-2S] ferredoxin produced in the heterocyst cells of Anabaena 7120 plays a key role in nitrogen fixation, where it serves as an electron acceptor from various sources and an electron donor to nitrogenase. Crystals of recombinant heterocyst ferredoxin, coded for by the fdx H gene from Anabaena 7120 and overproduced in Escherichia coli, have been grown from ammonium sulfate solutions and are suitable for high resolution X-ray crystallographic analysis. They belong to the hexagonal space group P6(1) or P6(5) with unit cell dimensions of a = b = 44.2 A and c = 80.6 A. The crystals contain one molecule per asymmetric unit and diffract to a nominal resolution of 1.6 A. The molecular structure of this heterocyst ferredoxin is of special interest in that 4 of the 22 amino acid positions thought to be absolutely conserved in nonhalophilic ferredoxins are different and, based on amino acid sequence alignments, three of these positions are located in the metal-cluster binding loop. Consequently, a high-resolution X-ray analysis of this [2Fe-2S] ferredoxin, and subsequent three-dimensional comparisons with other known ferredoxin models, will provide new insight into structure/function relationships for this class of redox proteins.
Jacobson
B L
BL
Institute for Enzyme Research, University of Wisconsin, Madison 53705.
Chae
Y K
YK
Böhme
H
H
Markley
J L
JL
Holden
H M
HM
D000645:Ammonium Sulfate; D017033:Anabaena / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003460:Crystallization; D005288:Ferredoxins / Q000737:chemistry*; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols; D011994:Recombinant Proteins / Q000737:chemistry; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
D005288:Ferredoxins; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; D000645:Ammonium Sulfate