[Diastereomers of nonionic oligonucleotide analogs. VI. Isolation of diastereomers of ethyl phosphotriesters of the octanucleotide d(GCCAAACA) by high performance affinity chromatography].
Diastereomers of oligonucleotide ethyl phosphotriesters were separated by high-performance complementary (affinity) chromatography on a column with the immobilized complementary oligonucleotide. The elution buffer contained 0.18 M K2HPO4, pH 7.5, and 30% acetonitrile. The temperature of the separation was a few degrees lower than Tm of corresponding oligonucleotide complexes. The diastereomers separated completely or partially were: d[GCC(Et)AAACA], d[GCCA(Et)AACA], d[GCAA(Et)ACA], d[GCC(Et)A(Et)AACA], d[GCC(Et)AA(Et)ACA], d[GCCA(Et)A(Et)ACA], d[GCC(Et)A(Et)A(Et)ACA].
Vtorushina
I A
IA
Gorbunov
Iu A
IuA
Zarytova
V F
VF
Komarova
N I
NI
Lebedev
A V
AV
Lokhov
S G
SG
Manaenko
A V
AV
Siniakov
A N
AN
Shishkina
I G
IG
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D013237:Stereoisomerism; D013696:Temperature
D009841:Oligonucleotides; D009943:Organophosphorus Compounds
Issedovanie diastereomerov neionnykh analogov oligonukleotidov. VI. Razdelenie diastereomerov étilovykh fosfotriéfirov oktanukleotida d(GCCAAACA) metodom vysokoéffektivnoĭ affinnoĭ khromatografii.