Invasive epithelial cells show more fast plasma membrane movements than related or parental non-invasive cells.
Fast plasma membrane movements (FPMM) are involved in ruffling, blebbing, fast shape change, and fast translocation. A simple method for the quantification of FPMM was used to study the relation between FPMM and invasive capacity in five pairs of invasive and noninvasive variants from four different epithelial cell types. The human mammary cell line MCF-7/6, the ras-transformed dog kidney cell line ras-MDCK, the ras-transformed mouse mammary gland cell lines NM9-ras-12 and NM-f-ras-TD, and spontaneously transformed late passage mouse lens explant MLE cells, all of which were invasive in vitro, showed more FPMM in our measurements and displayed more ruffling activity on time-lapse video films than the related or parental MCF-7/AZ, MDCK-3, NM9, and NM-f cell lines and early passage MLE cells, none of which were invasive. Interestingly, induction of invasive capacity in MCF-7/AZ cells by retinoic acid was accompanied by an increase in FPMM, but speed of translocation was not increased. Together these observations support the hypothesis that a certain level of FPMM is a prerequisite for invasive capacity.
van Larebeke
N A
NA
Department of Radiotherapy and Nuclear Medicine, University of Gent, Belgium.
Bracke
M E
ME
Mareel
M M
MM
D000818:Animals; D002460:Cell Line; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000187:drug effects / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D004285:Dogs; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009924:Organ Culture Techniques; D011554:Pseudopodia; D014212:Tretinoin / Q000494:pharmacology*; D014741:Video Recording / Q000379:methods
D014212:Tretinoin