Effects of terminal deletion mutations on function of the movement protein of tobacco mosaic virus.
A series of carboxy- and amino-terminal deletion mutations in the movement protein (MP) gene of tobacco mosaic virus (TMV) were ligated into a cloned TMV cDNA deleted for the endogenous MP gene. RNA transcripts were produced in vitro from clones carrying the various mutated MP genes. The effect of the deletion mutations on local and systemic movements of the infection was evaluated. Deletion of 9 or 33 amino acids from the carboxy terminus of the movement protein did not effect cell-to-cell movement as reflected by local lesion formation on Nicotiana tabacum cv. Xanthi NN plants. Deletion of 55 amino acids resulted in impaired MP that supported the formation of local lesions of 1 mm in diameter compared to lesions of 3-5 mm caused by the wild-type MP. Deletion of 74 amino acids (or more) from the carboxy terminus resulted in a protein that could not support virus movement. Modified viruses that contained repeated sequences in the 3' region of the MP gene lost the repeated sequences during replication and reverted to the wild type. This was evidenced by the size of the MP produced and by sequence analysis of reverse-transcribed PCR-amplified products, following infection by the modified virus. MP deleted for as few as 3 amino acids at the amino terminus could not support virus movement thus indicating that the amino-terminal domain is critical for MP activity.
Gafny
R
R
Department of Biology, Washington University, St. Louis, Missouri 63130.
Lapidot
M
M
Berna
A
A
Holt
C A
CA
Deom
C M
CM
Beachy
R N
RN
D002473:Cell Wall / Q000382:microbiology; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D053830:Plant Viral Movement Proteins; D010947:Plants, Toxic; D013329:Structure-Activity Relationship; D014026:Nicotiana / Q000382:microbiology; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity*; D014764:Viral Proteins / Q000235:genetics*
D053830:Plant Viral Movement Proteins; D014764:Viral Proteins