Transient expression in Physarum of a chloramphenicol acetyltransferase gene under the control of actin gene promoters.
We cloned and sequenced two actin promoters from Physarum, and constructed plasmids carrying these promoters upstream of a bacterial chloramphenicol acetyltransferase (cat) gene. We then tested the plasmids for their ability to express cat in Physarum amoebae. We present reliable methods for introducing plasmid DNA into Physarum amoebae by electroporation, and show that expression of the cat gene in amoebae occurs in the presence, but not the absence, of one or the other Physarum actin promoter.
Burland
T G
TG
McArdle Laboratory for Cancer Research, Madison, WI 53706.
Bailey
J
J
Adam
L
L
Mukhopadhyay
M J
MJ
Dove
W F
WF
Pallotta
D
D
D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002021:Buffers; D002463:Cell Membrane Permeability; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D004560:Electricity; D005821:Genetic Techniques; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010804:Physarum / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014162:Transfection
D000199:Actins; D002021:Buffers; D015500:Chloramphenicol O-Acetyltransferase