title: Involvement of histidine residues in catalytic activity of xanthine dehydrogenase from hen liver.
abstract: 1. Modification of histidine residue(s) of xanthine dehydrogenase from hen liver by DEP and photooxidation results in loss of the ability to transfer electrons from xanthine to NAD+ and also from NADH to 2,6-dichlorophenolindophenol (DCIP). 2. The kinetics of inactivation suggest that carbethoxylation of more than one histidyl residue in the enzyme may be responsible for the inactivation.
authors.affiliation: Department of Comparative Biochemistry, Polish Academy of Sciences, Warsaw.
authors.forename: B
authors.initials: B
authors.lastname: Zakrzewska
authors.forename: Z W
authors.initials: ZW
authors.lastname: Kamiński
mesh_terms: D015086:2,6-Dichloroindophenol / Q000378:metabolism; D000818:Animals; D002645:Chickens; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006639:Histidine / Q000737:chemistry*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010777:Photochemistry; D013329:Structure-Activity Relationship; D019820:Xanthine; D014968:Xanthine Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D014970:Xanthines / Q000378:metabolism
chemical_list: D014970:Xanthines; D009243:NAD; D019820:Xanthine; D006639:Histidine; D015086:2,6-Dichloroindophenol; D014968:Xanthine Dehydrogenase; D004047:Diethyl Pyrocarbonate