Effect of synthetic thymic humoral factor (THF-gamma 2) on T cell activities in immunodeficient ageing mice.
Immunodeficient ageing (C57BL/10 x DBA/2)F1 mice were treated by a single injection of synthetic thymic hormones and 4 days later their thymus and spleen cells were assayed in vitro for T cell activities. A few nanograms of THF-gamma 2 were found to raise the frequency of mitogen-responsive T cells in thymus and spleen cell populations as well as the frequency of cytokine-producing splenic T cells, up to the levels observed in young mice. Moreover, injection of THF-gamma 2 was found to restore T cell growth factor (TCGF) production by mitogen-stimulated spleen cells. Also, the helper activity of spleen cells was enhanced by this treatment and increased with increasing the THF-gamma 2 dose over a wide range. Similarly, the effects of thymopentin and thymosin-alpha 1 on T helper cell activity increased with increasing the injected dose, but the efficiencies of THF-gamma 2 and thymopentin were, respectively, 400-fold and eight-fold greater than that of thymosin-alpha 1.
Goso
C
C
Laboratory of Immunology, ENEA C.R.E. Casaccia, Rome, Italy.
Frasca
D
D
Doria
G
G
D000375:Aging / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D000077596:Thymalfasin; D016305:Thymopentin / Q000276:immunology; D013947:Thymosin / Q000031:analogs & derivatives* / Q000276:immunology; D013951:Thymus Hormones / Q000276:immunology*
D007376:Interleukin-2; D009842:Oligopeptides; D013951:Thymus Hormones; C064563:thymic humoral factor gamma 2; D013947:Thymosin; D016305:Thymopentin; D000077596:Thymalfasin