Evidence for a novel splice variant of the alpha subunit of Go in rat male haploid germ cells.
Northern analysis shows that Go alpha mRNA is highly expressed in immature germ cells from rat. Whereas alpha o2 mRNA is the major form in pachytene spermatocytes, a message of shorter chain length is present in large amount in haploid germ cells. This mRNA was detected with an oligonucleotide specific for the 3'-coding region of alpha o2, but did not hybridize to oligonucleotides specific for the 5'-untranslated and 5'-coding regions. The results indicate the presence of a novel splice variant of alpha o mRNA, which may code for a Go alpha protein important for germ cell development.
Haugen
T B
TB
Institute of Medical Biochemistry, University of Oslo, Norway.
Eskild
W
W
Hansson
V
V
D000818:Animals; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D019204:GTP-Binding Proteins / Q000235:genetics*; D014644:Genetic Variation; D006238:Haploidy; D008297:Male; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D012708:Sertoli Cells / Q000737:chemistry*; D013094:Spermatozoa / Q000737:chemistry*
D012333:RNA, Messenger; D019204:GTP-Binding Proteins; D019206:GTP-Binding Protein alpha Subunits, Gi-Go