title: Correlation between glycosylation of flagellin proteins and sensitivity of flagellar filaments to Triton X-100 in methanogens.
abstract: The flagellins of Methanospirillum hungatei strains JF1 and GP1, Methanococcus deltae, and Methanothermus fervidus are glycosylated. Isolated flagellar filaments from these organisms are dissociated by low concentrations (0.5% (v/v)) of Triton X-100. Flagellar filaments from other methanogens (Methanococcus voltae, Methanococcus vannielii and Methanoculleus marisnigri) composed of non-glycosylated flagellins are resistant to Triton X-100 treatment. Consequently, the isolation techniques (employing Triton X-100) used to isolate basal body-hook-filament complexes in eubacteria may not be applicable to many methanogens.
authors.affiliation: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
authors.forename: D M
authors.initials: DM
authors.lastname: Faguy
authors.forename: S F
authors.initials: SF
authors.lastname: Koval
authors.forename: K F
authors.initials: KF
authors.lastname: Jarrell
mesh_terms: D003902:Detergents / Q000494:pharmacology*; D005407:Flagella / Q000187:drug effects* / Q000648:ultrastructure; D005408:Flagellin / Q000378:metabolism*; D006031:Glycosylation / Q000187:drug effects; D017013:Methanobacteriaceae / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology*
chemical_list: D003902:Detergents; D005408:Flagellin; D011092:Polyethylene Glycols; D017830:Octoxynol