Polypeptide composition of the 8S form of prolyl-tRNA synthetase from rat liver.
Rat liver Fraction X containing the 24S complex of nine aminoacyl-tRNA synthetases, including prolyl-tRNA synthetase, was centrifuged on a 15-35% sucrose density gradient to obtain the 8S form of prolyl-tRNA synthetase. The enzyme was purified on a prolyldiaminohexyl-Sepharose 4B affinity column, specifically binding prolyl-tRNA synthetase to Sepharose-bound proline. After SDS-polyacrylamide gel electrophoresis, two peptides of 58 and 61 kDa were detected in the peak of prolyl-tRNA synthetase activity eluted from the affinity column. The 58 and 61 kDa peptides were also present in the 24S complex containing prolyl-tRNA synthetase activity isolated on the sucrose density gradient.
Bianchi
C
C
Institute of General Pathology, University of Milano, Italy.
Perego
R
R
Del Monte
U
U
D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008297:Male; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains
D046911:Macromolecular Substances; D010446:Peptide Fragments; D000604:Amino Acyl-tRNA Synthetases; C100134:prolyl T RNA synthetase