Evaluation of the genotoxic potential of sorbic acid and potassium sorbate.
The genotoxic potential of sorbic acid and potassium sorbate was investigated in vivo and in vitro. Oral administration of sorbic acid (up to 5000 mg/kg body weight) did not induce sister chromatid exchanges or the formation of micronuclei in bone marrow cells of mice. Intraperitoneal treatment of rats with 400-1200 mg potassium sorbate/kg body weight did not alter the elution profile of DNA from isolated liver cells in the in vivo alkaline elution assay. Sorbic acid did not induce DNA repair in cultured human A549 cells in the unscheduled DNA synthesis (UDS) assay. In vitro incubation of the cells with 1-1000 micrograms potassium sorbate/ml, in the absence or presence of rat liver homogenate, did not result in the formation of DNA single-strand breaks in the alkaline elution assay. These results demonstrate that sorbic acid and its potassium salt are not genotoxic in vivo or in vitro. In contrast to sorbic acid and potassium sorbate, sodium sorbate is very sensitive to oxidative degradation; the main oxidation product was identified to be 4,5-oxohexenoate, which was mutagenic in the Ames test.
Jung
R
R
Hoechst AG, Frankfurt, Germany.
Cojocel
C
C
Müller
W
W
Böttger
D
D
Lück
E
E
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004277:DNA, Single-Stranded / Q000096:biosynthesis; D004355:Drug Stability; D005260:Female; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D051381:Rats; D011919:Rats, Inbred Strains; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013011:Sorbic Acid / Q000633:toxicity*
D004277:DNA, Single-Stranded; D013011:Sorbic Acid