31P nuclear magnetic resonance study of phospholipid metabolites in ischemic liver.
To assess the metabolic alterations induced by normothermic hepatic ischemia, 31P nuclear magnetic resonance analysis was performed on liver samples using perchloric acid extraction. In particular, phosphomonoesters and phosphodiesters, the intermediary metabolites of membrane phospholipid turnover, were characterized precisely and quantitated. Phosphocholine and phosphoethanolamine, the precursors of phospholipid anabolism, did not change, while the phosphodiesters decreased. In contrast, alpha-glycerophosphate, which is both a precursor of phospholipid synthesis and the intermediary product of phospholipid degradation, markedly increased following 30 min of normothermic ischemia. These findings suggest that cellular phospholipids are actively degraded during normothermic hepatic ischemia.
Hachisuka
T
T
Department of Surgery II, Nagoya University School of Medicine, Japan.
Nakayama
S
S
Tomita
T
T
Takagi
H
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D005994:Glycerophosphates / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D009711:Nucleotides / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*
D005994:Glycerophosphates; D009711:Nucleotides; D010710:Phosphates; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D000255:Adenosine Triphosphate; C029620:alpha-glycerophosphoric acid