[Determination of nucleolar organizer activity in the differential diagnosis of severe dysplasia and cancer of the large intestine].
The method is based on the selective nucleolar staining with silver salts, the number of silver granules serves as an index of the nucleolar organizer activity which corresponds to the number of RNA-polymerases acting in the cell. The number of silver granules is 5.7, 6.3 and 6.8 per cell in mild, moderate and severe dysplasia, respectively. The histogram of the cell distribution, with regard to the silver grain number, in severe dysplasia is characterized by a shift to the left, and by one high peak. The average number of silver grains is significantly increased in non-invasive and invasive carcinoma and reaches the values of 18.8 and 20.4. A histogram in carcinoma is pulled to the right due to the cells with high content of silver grains and has many low peaks this reflecting variability of cells as to the silver grain content.
Koshkina
L I
LI
Neĭshtadt
E L
EL
Mamaev
N N
NN
D000236:Adenoma / Q000737:chemistry / Q000175:diagnosis*; D002277:Carcinoma / Q000737:chemistry / Q000175:diagnosis*; D012321:DNA-Directed RNA Polymerases / Q000032:analysis; D003937:Diagnosis, Differential; D006801:Humans; D007414:Intestinal Neoplasms / Q000737:chemistry / Q000175:diagnosis*; D007420:Intestine, Large / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009697:Nucleolus Organizer Region / Q000737:chemistry*; D016622:Silver Staining
D012321:DNA-Directed RNA Polymerases
Opredelenie aktivnosti iadryshkovykh organizatorov pri differentsial'noĭ diagnostike tiazheloĭ displazii i kartsinomy tolstoĭ kishki.