Failure of passive transfer of serum from patients with alopecia areata and alopecia universalis to inhibit hair growth in transplants of human scalp skin grafted on to nude mice.
We have previously demonstrated regrowth of hair in scalp skin grafts taken from patients with alopecia areata (AA) and alopecia universalis (AU) following engraftment on to nude mice. This present study was to determine whether serum from patients with AA and AU, has a role in the process of hair loss and the role of antibodies and complement. Forty mice were grafted with transplants obtained from seven patients. One group of the grafted mice was given patients' serum and another group normal serum. The mice were treated topically with cyclosporin (CyA), or olive oil. Hair growth was noted in most grafts and intravenous injections of serum did not prevent or inhibit this process. Immunofluorescence studies before grafting showed deposition of immunoglobulins and complement in hair follicles in both normal and affected scalp skin, but a more striking deposition was noted in the affected skin. Deposition of immunoreactants after grafting was observed only after the injection of serum from the patients but not with normal serum. Thus the sera from patients with AA or AU, when injected into nude mice with hair transplants from the scalp skin of patients with these disorders, does not alter the hair growth despite deposition of immunoreactants around the hair follicles.
Gilhar
A
A
Skin Research Laboratory, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
Pillar
T
T
Assay
B
B
David
M
M
D000505:Alopecia / Q000276:immunology*; D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology*; D003165:Complement System Proteins / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D006197:Hair / Q000254:growth & development*; D006801:Humans; D007116:Immunization, Passive; D007136:Immunoglobulins / Q000032:analysis; D008297:Male; D051379:Mice; D008819:Mice, Nude; D012535:Scalp; D012867:Skin / Q000276:immunology; D016038:Skin Transplantation / Q000276:immunology*
D007136:Immunoglobulins; D003165:Complement System Proteins