title: Relationship between sensitivity to natural killer cells and MHC class-I antigen expression in colon carcinoma cell lines.
abstract: The sensitivity of colorectal tumors to NK-cell-mediated cytotoxicity and their expression of major histocompatibility complex (MHC) class-I antigens were studied in an attempt to determine whether such antigens play a role in the susceptibility of colorectal tumors to NK-cell lysis. In a rat colon-carcinoma model, 2 clones differing in their sensitivity to NK-cell-mediated cytotoxicity were tested for class-I expression; it was seen that the more sensitive cells (REGb) expressed less class-I products than did the resistant cells (PROb). However, when MHC class-I antigen expression was increased by IFN-gamma treatment, no change in NK-cell lysis was found with the PROb cells, while an increase in cytotoxicity was obtained with the REGb cells. After in vivo or in vitro selection of NK-resistant REGb cells, we observed in the selected cells an important decrease in RT-I class-I antigen expression. Fifteen different human colorectal cell lines were also studied for HLA class-I expression and NK-cell susceptibility, and no quantitative correlation between these 2 features was seen. However, cell lines which were deficient in HLA class-I antigens were more sensitive than class-I-positive cells.
authors.affiliation: CJF INSERM 90.11, Faculté de Médecine, Nantes, France.
authors.forename: H M
authors.initials: HM
authors.lastname: Blottière
authors.forename: R
authors.initials: R
authors.lastname: Zennadi
authors.forename: C
authors.initials: C
authors.lastname: Burg
authors.forename: J Y
authors.initials: JY
authors.lastname: Douillard
authors.forename: K
authors.initials: K
authors.lastname: Meflah
authors.forename: J
authors.initials: J
authors.lastname: Le Pendu
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000276:immunology*; D003110:Colonic Neoplasms / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I