Regional distribution of DARPP-32 (dopamine- and adenosine 3',5'-monophosphate-regulated phosphoprotein of Mr = 32,000) mRNA in mouse brain.
DARPP-32 (dopamine- and adenosine 3',5'-monophosphate-regulated phosphoprotein of Mr = 32,000) mRNA distribution was examined in adult mouse central nervous system by in situ hybridization. In general, DARPP-32 mRNA was found in regions of brain where cells express the dopamine D1 subtype receptor. Cells of the olfactory tubercle, caudate-putamen, and nucleus accumbens had the highest levels of DARPP-32 mRNA, as did choroid plexus and Purkinje cells. Relatively high levels were found in medial habenula and lateral piriform cortex. Moderate levels were seen in cerebral cortex layer VI, medial piriform cortex, lateral entorhinal cortex, tenia tecta, anterior olfactory nucleus, and lateral bed nucleus of the stria terminalis. Low levels were observed in hippocampus, cerebral cortex layers II and III, olfactory bulb, and the nucleus of the lateral olfactory tract. DARPP-32 mRNA levels in the amygdaloid nuclei varied greatly.
Perez
R G
RG
Department of Neurobiology, Anatomy and Cell Science, University of Pittsburgh, School of Medicine, Pennsylvania 15261.
Lewis
R M
RM
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000033:anatomy & histology; D001923:Brain Chemistry / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D066298:In Vitro Techniques; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009693:Nucleic Acid Hybridization; D010750:Phosphoproteins; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D011954:Receptors, Dopamine / Q000378:metabolism; D017447:Receptors, Dopamine D1
D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D015347:RNA Probes; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D004247:DNA