Cloning and characterization of a gene encoding extracellular metalloprotease from Streptomyces lividans.
The prt gene, encoding a protease (Prt) from Streptomyces lividans TK24, was cloned and sequenced. An S. lividans host with plasmid-borne prt secreted 200 micrograms/ml of a 22-kDa Prt into the culture medium. Prt is classified as a metalloprotease since its activity is significantly inhibited by 1,10-phenanthroline or EDTA. The region upstream from prt codes for an incomplete open reading frame (ORF) oriented opposite to prt. This ORF has a strong similarity to a gene family (lysR) whose members regulate the transcription of structural genes required for either biosynthesis or degradation.
Lichenstein
H S
HS
Amgen Inc., Thousand Oaks, CA 91320.
Busse
L A
LA
Smith
G A
GA
Narhi
L O
LO
McGinley
M O
MO
Rohde
M F
MF
Katzowitz
J L
JL
Zukowski
M M
MM
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D015698:Genomic Library; D006863:Hydrogen-Ion Concentration; D008666:Metalloendopeptidases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D013696:Temperature
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008666:Metalloendopeptidases; C073584:Prt protein, Streptomyces