Kinetics of phosphofructokinase from granulocytes isolated from patients with insulin resistance.
Phosphofructokinases from granulocytes isolated from insulin-resistant patients, mainly those from type II diabetics where the degree of insulin resistance was more pronounced, exhibit some changes in their kinetic behavior when assayed under allosteric conditions, characterized by an increased affinity for fructose-6-phosphate, being more resistant to ATP inhibition while it became more sensitive to citrate inhibitory effect. Those changes are suggestive of a isozymic modification to a more L-type enriched enzyme with a loss of the F-type component, probably present in the normal granulocyte.
Campos
G
G
Instituto de Investigaciones Clínicas, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela.
Ryder
E
E
Morales
L M
LM
Raleigh
X
X
D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000494:Allosteric Regulation; D002951:Citrates / Q000494:pharmacology; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology; D005636:Fructosephosphates / Q000378:metabolism; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007333:Insulin Resistance; D007700:Kinetics; D009765:Obesity / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000037:antagonists & inhibitors / Q000378:metabolism*
D002951:Citrates; D005636:Fructosephosphates; C027618:fructose-6-phosphate; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1