title: Evolution of ruminant hemoglobins. Thermodynamic divergence of ox and buffalo hemoglobins.
abstract: The ligand-binding properties of hemoglobins from two homozygote phenotypes (AA and BB) of water buffalo (Bubalus bubalis) have been characterized by equilibrium and kinetic techniques. In the case of the BB phenotype, the two constituent hemoglobins have been purified and separately analysed. Buffalo hemoglobins display the reduced sensitivity to organic phosphates characteristic of ruminant hemoglobins, their physiological effector probably being the chloride ion. In contrast to the other known hemoglobins from ruminants, all the hemoglobins from the water buffalo display a significant temperature sensitivity, the delta H for oxygen binding in the presence of physiological effectors approaching that of human hemoglobin (delta H = -30.5 kJ/mol O2). This discrepancy with the other ruminant hemoglobins (e.g. ox, delta H = -10.4 kJ/mol O2), whose primary structure is very similar to that of buffalo, hemoglobins might be correlated to the different habitat and phylogenetic history of the two subfamilies (Bos and Bubalus) of Bovidae.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Rome, Tor Vergata, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Giardina
authors.forename: F
authors.initials: F
authors.lastname: Arevalo
authors.forename: M E
authors.initials: ME
authors.lastname: Clementi
authors.forename: L
authors.initials: L
authors.lastname: Ferrara
authors.forename: A
authors.initials: A
authors.lastname: Di Luccia
authors.forename: E
authors.initials: E
authors.lastname: Lendaro
authors.forename: A
authors.initials: A
authors.lastname: Bellelli
authors.forename: S G
authors.initials: SG
authors.lastname: Condò
mesh_terms: D000818:Animals; D006454:Hemoglobins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D010641:Phenotype; D012418:Ruminants / Q000235:genetics*; D013816:Thermodynamics
chemical_list: D006454:Hemoglobins; D010100:Oxygen