title: The biochemical basis of secondary ischemia.
abstract: In this study rat epigastric island flaps were used as a model to investigate selected tissue biochemical changes occurring during secondary ischemia. It was hypothesized that free radical damage, depletion of free radical scavengers, depletion of ATP, and increased edema might explain differences in flap survival between partial (venous obstruction) and total (arteriovenous obstruction) ischemia and decreased flap survival with increasing ischemia time. Flaps were given 2 hr or primary ischemia, 8 hr of normal perfusion, then secondary ischemia of 0, 2, 4, 8, or 12 hr with either arteriovenous obstruction or venous obstruction. Biochemical analysis of the skin was performed after 0, 24, or 96 hr reperfusion. Only minor differences were found between arteriovenous and venous ischemia for any of five biochemical parameters, despite a previous finding that venous ischemic flaps are more susceptible to necrosis. Levels of xanthine oxidase and malonyldialdehyde (both indices of free radical generation) increased with ischemia time. Levels of superoxide dismutase (a free radical scavenger) correspondingly decreased. Tissue levels of ATP decreased after ischemia and recovered to normal for shorter but not for longer ischemia times after 96 hr of reperfusion in parallel with flap survival. Edema increased immediately after the ischemic insult but decreased once the tissue became necrotic. These results imply roles for free radicals, ATP, and edema in secondary ischemia, but do not distinguish between arteriovenous and venous secondary ischemia.
authors.affiliation: Microsurgery Research Centre, St. Vincent's Hospital, Melbourne, Australia.
authors.forename: C G
authors.initials: CG
authors.lastname: Mellow
authors.forename: K R
authors.initials: KR
authors.lastname: Knight
authors.forename: M F
authors.initials: MF
authors.lastname: Angel
authors.forename: S A
authors.initials: SA
authors.lastname: Coe
authors.forename: B M
authors.initials: BM
authors.lastname: O'Brien
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003250:Constriction; D004487:Edema / Q000209:etiology; D007511:Ischemia / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013524:Surgical Flaps / Q000502:physiology; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase