Disaccharide analysis of the skin glycosaminoglycans in systemic sclerosis.
The disaccharides constituting chondroitinase-digestible glycosaminoglycan (GAG) in the skin lesions of patients with systemic sclerosis were determined using high-performance liquid chromatography (HPLC). In scleroderma there was an increase in the amount of delta Di-4S(DS), the main disaccharide unit of dermatan sulphate, and a decrease in delta Di-HA, the disaccharide unit of hyaluronic acid, as compared with normal skin from a similar site. The distribution pattern of the main disaccharides constituting chondroitin sulphate and dermatan sulphate in scleroderma differed from that in scars or scleredema.
Akimoto
S
S
Department of Dermatology, School of Medicine, Gunma University, Maebashi, Japan.
Hayashi
H
H
Ishikawa
H
H
D000328:Adult; D000368:Aged; D002809:Chondroitin Sulfates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003871:Dermatan Sulfate / Q000032:analysis; D004187:Disaccharides / Q000032:analysis*; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis; D008297:Male; D008875:Middle Aged; D012595:Scleroderma, Systemic; D012867:Skin / Q000737:chemistry*
D004187:Disaccharides; D006025:Glycosaminoglycans; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates