E-rosette formation using heteropolymeric monoclonal antibodies.
We have used bispecific, cross-linked monoclonal antibodies (heteropolymers, HP) to facilitate rosette formation between human erythrocytes (EH) and dinitrophenylated sheep erythrocytes (DNP-ES) in the absence of complement. The HP contain monoclonal antibodies (mAbs) specific for both the EH C3b receptor (CR1), and the DNP group, and control experiments with homologous competing non-cross-linked mAbs and naive EH and ES confirm the specificity of the rosetting reaction. These results extend our previous studies, of HP-mediated binding of simple protein antigens to EH CR1, to complex particulate antigens and may eventually allow for the targeting and clearance from the circulation of a variety of pathogens associated with infectious disease.
Labuguen
R H
RH
Department of Biochemistry, University of Virginia School of Medicine, Charlottesville 22908.
Taylor
R P
RP
Zimmermann
R L
RL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004136:Dinitrobenzenes; D004912:Erythrocytes; D006801:Humans; D011108:Polymers; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D012397:Rosette Formation; D012756:Sheep
D000911:Antibodies, Monoclonal; D004136:Dinitrobenzenes; D011108:Polymers; D011951:Receptors, Complement; D017463:Receptors, Complement 3b