Modulatory influence of injectable contraceptive steroid medroxyprogesterone acetate on methylcholanthrene-induced carcinogenesis in the uterine cervix of mouse.
Methylcholanthrene (approximately 300 micrograms) plus beeswax-impregnated thread, when placed inside the canal of the uterine cervix of virgin female adult mice for 30, 60 and 90 days produced cervical tumors in 0.0, 10 and 30% of mice, respectively. Employing this experimental cervical carcinogenesis model system, the present study evaluated the modulatory influence of medroxyprogesterone acetate (MPA) on the incidences of precancerous and cancerous lesions in the cervical epithelium as well as on phase I and phase II drug metabolizing enzymes and acid soluble sulfhydryl level in the liver. Intramuscular administration of MPA (50 micrograms every 5th day) to the carcinogen-thread inserted mice for 30, 60 and 90 days produced cervical tumors respectively in 0.0, 13.3 and 60.5% (P less than 0.05) of mice. A significant increase (P less than 0.05) in hyperplasia was also observed in the present study. A significant decrease in cytochrome b5 was found after 30 days.
Hussain
S P
SP
Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Rao
A R
AR
D000282:Administration, Intravaginal; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003271:Contraceptive Agents, Female / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects / Q000378:metabolism; D015786:Cytochromes b5 / Q000187:drug effects / Q000378:metabolism; D004347:Drug Interactions; D005260:Female; D005982:Glutathione Transferase / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008525:Medroxyprogesterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D017258:Medroxyprogesterone Acetate; D008748:Methylcholanthrene; D051379:Mice; D013438:Sulfhydryl Compounds / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000139:chemically induced*
D003271:Contraceptive Agents, Female; D013438:Sulfhydryl Compounds; D008748:Methylcholanthrene; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D017258:Medroxyprogesterone Acetate; D005982:Glutathione Transferase; D008525:Medroxyprogesterone