Phenotypic profile and functional characteristics of human gamma and delta T cells during acute toxoplasmosis.
Gamma and delta (gamma delta) T-cell receptor lymphocytes are increased during acute toxoplasmosis. These cells are BB3+ CD45RO+ CD8-. Purified gamma delta T cells failed to proliferate in response to Toxoplasma gondii antigen (stimulation index, 1.4 +/- 0.6) but were responsive to phytohemagglutinin stimulation (stimulation index, 20.8 +/- 1.9). Natural-killer-like cytotoxicity was strongly acquired only after in vitro culture of purified gamma delta T cells with recombinant interleukin 2 (40% +/- 7% specific lysis). Our data show that gamma delta T-cell receptor T cells with a peculiar phenotype are increased during human acute T. gondii infection.
De Paoli
P
P
Department of Microbiology and Immunology, General Hospital USL11, Pordenone, Italy.
Basaglia
G
G
Gennari
D
D
Crovatto
M
M
Modolo
M L
ML
Santini
G
G
D000208:Acute Disease; D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008297:Male; D010641:Phenotype; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology; D014123:Toxoplasmosis / Q000276:immunology*
D000953:Antigens, Protozoan; D016692:Receptors, Antigen, T-Cell, gamma-delta