Schwannoma-derived growth factor promotes the neuronal differentiation and survival of PC12 cells.
Schwannoma-derived growth factor (SDGF) was initially isolated from schwannoma cells as a mitogen for glial cells and fibroblasts. The present data show that SDGF causes the morphological and molecular differentiation of rat PC12 cells in a manner similar to, but distinguishable from nerve growth factor (NGF). It also promotes PC12 survival in serum-free conditions. SDGF induced changes include neurite outgrowth and the induction of the mRNAs for GAP-43 and transin, proteins which are highly expressed in axons. In addition, both SDGF and NGF induce the transcription factor, NGFI-A. The time course of the response to SDGF is similar to that for NGF. Gap-43 mRNA induction by both SDGF and NGF is inhibited by dexamethasone, but dexamethasone has no effect on NGFI-A mRNA synthesis. These observations show that SDGF has a differentiation and survival promoting effect on PC12 cells in addition to its mitogenic activity on glial cells and fibroblasts.
Kimura
H
H
Salk Institute, San Diego, California 92186-5800.
Schubert
D
D
D066258:Amphiregulin; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D066255:EGF Family of Proteins; D051766:Early Growth Response Protein 1; D019922:GAP-43 Protein; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins; D006133:Growth Substances / Q000494:pharmacology*; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D019278:Matrix Metalloproteinase 3; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000235:genetics; D009414:Nerve Growth Factors / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D016501:Neurites / Q000648:ultrastructure*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D016716:PC12 Cells; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics
D066258:Amphiregulin; C587850:Areg protein, rat; D003470:Culture Media; D004268:DNA-Binding Proteins; D066255:EGF Family of Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D019922:GAP-43 Protein; D006023:Glycoproteins; D006133:Growth Substances; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D003907:Dexamethasone; D003513:Cycloheximide; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3