Mutational analysis of a variant of ARS1 from Saccharomyces cerevisiae.
A naturally occurring single base-pair G to A transition, creating a 10/11 near-match close to the essential 11 base-pair core consensus of ARS1, was used to investigate the importance of near-match sequences. The 10/11 near-match can not substitute for the core consensus since an ARS- phenotype is observed when the core consensus is deleted. However, deletion mutations revealed that this near-match together with a short palindromic sequence, also situated in the B-flanking region, comprise a single element crucial for optimal ARS function. The palindrome has the potential of forming a stem-loop structure. Rather precise observations concerning the borders of the B-region were achieved. The four base pairs separating the near-match from the core consensus perform a spacing function where the identity of the bases are unimportant. However, this spacing is highly important since deletion of these four base pairs leads to an ARS- phenotype.
Kirpekar
F
F
Department of Molecular Biology, Odense University, Denmark.
Gulløv
K
K
D001483:Base Sequence; D004252:DNA Mutational Analysis; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal; D004277:DNA, Single-Stranded