Polylysine activates smooth muscle actin-myosin interaction without LC20 phosphorylation.
Phosphorylation/dephosphorylation of the 20-kDa light chain of smooth muscle myosin is a major regulator of actin-myosin interaction. Phosphatase inhibitors have thus been shown to enhance contraction in smooth muscle. The activity of type II phosphatase against phosphorylated myosin light chains is inhibited by polylysine. Thus we studied the effects of polylysine (10-13 kDa) on actin-myosin interaction in permeabilized guinea pig taenia coli fibers and in bovine aortic actomyosin. Addition of polylysine (10-20 microM) to Ca-ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid buffered solution ([Ca2+] less than 0.01 microM) elicited a contraction in fibers of 40 +/- 8% (n = 6) of maximally stimulated contractions ([Ca2+] congruent to 1.5 microM). Untreated fibers did not generate any significant force in parallel control experiments. Similarly, polylysine stimulated the ATPase activity both in fibers and actomyosin in a dose-dependent manner. This stimulation could be completely inhibited and abolished upon addition of heparin, a negatively charged heteropolysaccharide. In actomyosin previously phosphorylated with ATP gamma S, polylysine in a concentration range of 2-13 microM did not further stimulate enzyme activity. These increases in activity were not connected with significant changes in the phosphorylation of 20-kDa myosin light chain nor could any incorporation of 32P associated with polylysine stimulation be detected in both skinned fibers and actomyosin by autoradiography of SDS gels. Our data indicate that polylysine increases actin-myosin interaction in both smooth muscle model systems by directly influencing contractile proteins. As such, polylysine may be a useful probe for the mechanism of activation of smooth muscle.
Szymanski
P T
PT
Department of Physiology and Biophysics, College of Medicine, University of Cincinnati, Ohio 45267-0576.
Strauss
J D
JD
Doerman
G
G
DiSalvo
J
J
Paul
R J
RJ
D000199:Actins / Q000378:metabolism*; D000205:Actomyosin / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D002417:Cattle; D003106:Colon / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D009130:Muscle, Smooth / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009218:Myosins / Q000378:metabolism*; D010766:Phosphorylation; D011107:Polylysine / Q000378:metabolism*
D000199:Actins; D002147:Calmodulin; D011107:Polylysine; D004533:Egtazic Acid; D000205:Actomyosin; D009218:Myosins; D002118:Calcium