Constitutive expression of c-fos gene inhibits type 1 collagen synthesis in transfected osteoblasts.
To clarify the contribution of c-fos DNA to bone formation, the effect of constitutive expression of the c-fos gene in collagen synthesis was examined by introducing c-fos DNA into osteoblastic MC3T3-E1 cells. The [3H] proline incorporation into the collagenase digestible protein(CDP) and the percent collagen synthesis were significantly decreased in the c-fos transfectants which constitutively express c-fos mRNA as compared with control transfectants. Transcription of type 1(alpha 1) collagen gene was also specifically decreased in the c-fos transfectants. This indicates that constitutive expression of c-fos DNA interferes with bone formation by inhibiting collagen synthesis in osteoblasts.
Kuroki
Y
Y
Department of Medicine, Kobe University School of Medicine, Japan.
Shiozawa
S
S
Sugimoto
T
T
Fujita
T
T
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D003094:Collagen / Q000096:biosynthesis*; D003360:Cosmids; D003470:Culture Media; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D016762:Genes, fos; D005822:Genetic Vectors; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000502:physiology*; D011392:Proline / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D003470:Culture Media; D012333:RNA, Messenger; D003094:Collagen; D004247:DNA; D011392:Proline