Further characterization of monoclonal antibody indicates specificity for (6-4)-dipyrimidine photoproducts.
Monoclonal antibody aUVssDNA-1 is produced by hybridoma cell line 25JF.C3B6 originally selected from cell fusions using spleen cells from mice immunized with UV-irradiated polydeoxynucleotides (Strickland and Boyle, Photochem. Photobiol. 34, 595-601, 1981). Original and subsequent studies of the binding characteristics of aUVssDNA-1 indicated that it was specific for cyclobuta-dithymidine photoproducts. Those investigations examined action spectrum, short-wavelength photo-reversal, nucleotide sequence effects, and photoreactivation using E. coli photolyase and incandescent light. However, the more recent studies reported here examined acetophenone-UV-B photosensitization, UV-B photoisomerization, and photoreactivation using cloned E. coli photolyase and filtered incandescent light. The results indicate that aUVssDNA-1 recognizes photoproducts with characteristics of (6-4)-dipyrimidines. Thus, previous studies in which relatively rapid repair of cyclobuta-dithymidine photoproducts was inferred using this antibody, require re-interpretation in light of these new findings.
Strickland
P T
PT
Johns Hopkins School of Hygiene and Public Health, Baltimore, MD 21205.
Nikaido
O
O
Matsunaga
T
T
Boyle
J M
JM
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004277:DNA, Single-Stranded / Q000276:immunology / Q000528:radiation effects; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000276:immunology* / Q000528:radiation effects; D014466:Ultraviolet Rays
D000911:Antibodies, Monoclonal; D004277:DNA, Single-Stranded; D011740:Pyrimidine Dimers