HIV-1 protease cleaves actin during acute infection of human T-lymphocytes.
Actin, one of the most abundant proteins of the cell, is hydrolyzed by the human immunodeficiency virus type 1 (HIV-1) protease during acute infection of cultured human T lymphocytes. The actin fragments produced during the course of infection are identical to those obtained by recombinant HIV-1 protease digests of (1) a lysate from uninfected T lymphocytes and (2) globular actin itself. Hydrolysis by the HIV-1 protease of physiologically important host cellular proteins during infection may have important consequences relative to viral pathogenesis.
Adams
L D
LD
Biochemistry Unit, Upjohn Laboratories, Kalamazoo, MI 49001.
Tomasselli
A G
AG
Robbins
P
P
Moss
B
B
Heinrikson
R L
RL
D000199:Actins / Q000378:metabolism*; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D016333:HIV Protease / Q000378:metabolism*; D015497:HIV-1 / Q000201:enzymology* / Q000502:physiology; D006801:Humans; D006868:Hydrolysis; D013601:T-Lymphocytes / Q000382:microbiology
D000199:Actins; D016333:HIV Protease