Down-regulation of adenylate cyclase-coupled response to native bovine parathyroid hormone and fragments in the osteoblast-like cell line UMR-106.
1. Parathyroid hormone-induced down-regulation was studied in the osteosarcoma cell line UMR-106. 2. A maximal priming does of bPTH (1-84) down-regulated PTH-responsiveness to 40% of its initial value; bPTH (1-41) was less effective than bPTH (1-84), whereas bPTH (42-84) had no effect, alone or in combination with bPTH (1-41). 3. A tentative model for the function of different domains of parathyroid hormone in down-regulation is suggested.
Woods
D B
DB
St Bartholomews Hospital, London, England.
Thangavelu
V
V
Barling
P M
PM
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D010006:Osteoblasts / Q000187:drug effects / Q000201:enzymology*; D012516:Osteosarcoma; D010281:Parathyroid Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D010281:Parathyroid Hormone; D010446:Peptide Fragments; D003907:Dexamethasone; D000262:Adenylyl Cyclases