Specific binding of influenza A virus NS1 protein to the virus minus-sense RNA in vitro.
The non-structural protein NS1, encoded by genome segment 8 of influenza A virus, was expressed in Escherichia coli from cloned cDNA and purified. The NS1 protein had a specific RNA-binding activity, binding to influenza A virus minus-sense but not plus-sense RNA synthesized in vitro from cloned DNA using phage RNA polymerase. NS1 bound preferentially to the regions of RNA containing either 5'- or 3'-terminal common sequences of the genomic RNA. Binding was inhibited by virion RNA, but not by single-stranded minus-sense cDNA and oligo DNAs having the common sequences. In addition, binding was also inhibited by 28S rRNA but not 18S rRNA prepared from MDCK cells.
Hatada
E
E
Department of Biochemistry, Kanazawa University School of Medicine, Japan.
Takizawa
T
T
Fukuda
R
R
D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D016372:RNA, Antisense / Q000378:metabolism*; D012337:RNA, Ribosomal, 18S / Q000378:metabolism; D012339:RNA, Ribosomal, 28S / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014758:Viral Core Proteins / Q000378:metabolism*; D017361:Viral Nonstructural Proteins
D016372:RNA, Antisense; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D012367:RNA, Viral; D011994:Recombinant Proteins; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins