Effects of brefeldin A on the synthesis of chondroitin 4-sulfate by cultures of mouse mastocytoma cells.
Mouse mastocytoma cells were cultured with brefeldin A in medium containing [35S]sulfate and [3H]glucosamine in order to determine the effects of this fungal metabolite on the formation of chondroitin 4-sulfate by these cells. There was a marked reduction in the incorporation of [35S]sulfate into the glycosaminoglycan which was approximately equal to the reduction in the incorporation of [3H]hexosamine into the same molecule. The chondroitin 4-sulfate chain size was greatly diminished, while the number of chains appeared to remain relatively constant, indicating that the brefeldin A partially disrupted the polymerizing system, but had little effect upon movement of the nascent proteochondroitin to the site for chondroitin polymerization and sulfation.
Sugumaran
G
G
Connective Tissue Laboratory, Department of Veterans Affairs Outpatient Clinic, Boston, MA 01730.
Katsman
M
M
Silbert
J E
JE
D000818:Animals; D020126:Brefeldin A; D002809:Chondroitin Sulfates / Q000096:biosynthesis*; D003517:Cyclopentanes / Q000494:pharmacology*; D005944:Glucosamine / Q000378:metabolism; D012515:Mast-Cell Sarcoma / Q000378:metabolism*; D051379:Mice; D009183:Mycotoxins / Q000494:pharmacology; D013431:Sulfates / Q000378:metabolism; D014407:Tumor Cells, Cultured
D003517:Cyclopentanes; D009183:Mycotoxins; D013431:Sulfates; D020126:Brefeldin A; D002809:Chondroitin Sulfates; D005944:Glucosamine