Identification of Candida albicans antigens reactive with immunoglobulin E antibody of human sera.
Candida albicans antigens which reacted with immunoglobulin E (IgE) antibodies of 57 allergic patients were detected by immunoblotting. Of the various antigens, the 175-, 125-, 46-, 43-, and 37-kDa antigenic components reacted most frequently with the patient sera. To purify the major antigens, C. albicans cells were fractionated. The 46-, 43-, and 37-kDa antigens were recovered in cytoplasmic fractions, but the 175- and 125-kDa antigens were not recovered in any fraction. The 46-, 43-, and 37-kDa antigens were purified from cytoplasmic fractions by DEAE and P11 ion-exchange chromatography. Antigens were isolated by cutting bands out of sodium dodecyl sulfate-polyacrylamide gels. The purified components confirmed by immunoblotting were next processed for amino acid sequencing. Parts of the sequences of the 46-, 43-, and 37-kDa antigens had significant levels of homology with Saccharomyces cerevisiae glycolytic enzyme enolase, phosphoglycerate kinase, and aldolase, respectively. Rabbit IgG antibodies prepared against the 46- and 43-kDa antigens strongly cross-reacted with the homologous proteins of S. cerevisiae. However, S. cerevisiae enolase and phosphoglycerate kinase did not cross-react with IgE of patient sera. This result suggests that IgE antibodies against only small parts of their epitopes are elevated in the allergic patients. Since enolase is reported to be a major antigen for systemic candidiasis, this enzyme may be the immunodominant protein in both allergies and fungal infections.
Ishiguro
A
A
Laboratory of Medical Mycology, Nagoya University School of Medicine, Japan.
Homma
M
M
Torii
S
S
Tanaka
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000946:Antigens, Fungal / Q000032:analysis* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001249:Asthma / Q000276:immunology; D015153:Blotting, Western; D002176:Candida albicans / Q000276:immunology*; D002458:Cell Fractionation; D002648:Child; D002675:Child, Preschool; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003488:Cyanogen Bromide; D003876:Dermatitis, Atopic / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D011852:Radioallergosorbent Test; D012689:Sequence Homology, Nucleic Acid
D000946:Antigens, Fungal; D007073:Immunoglobulin E; D003488:Cyanogen Bromide