title: Production of endothelin-1 and big-endothelin-1 by pleural mesothelial cells.
abstract: Immunoreactive endothelin-1 (ET-1) and big-endothelin-1 (big-ET-1) were detected in conditioned medium of cultured rat pleural mesothelial cells by using sensitive sandwich-type enzyme immunoassay. The amount of both ET-1 and big-ET-1 increased time-dependently. In both instances, maximal amount was detectable in conditioned medium obtained after 72 h in culture (ET-1: 117.1 +/- 30.1 pg/10(6) cells; big-ET-1: 2.4 +/- 2.1 pg/10(6) cells). Fetal calf serum markedly stimulated the production of both ET-1 and big-ET-1.
authors.affiliation: Department of Comparative Pathophysiology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kuwahara
authors.forename: M
authors.initials: M
authors.lastname: Kuwahara
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D019332:Endothelin-1; D016232:Endothelins / Q000032:analysis / Q000096:biosynthesis*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D010994:Pleura / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011498:Protein Precursors / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology
chemical_list: D003470:Culture Media; D019332:Endothelin-1; D016232:Endothelins; D011498:Protein Precursors; D012710:Serum Albumin, Bovine