Correlation between epidermal growth factor receptor concentration and the growth of human gastric cancer xenografts in nude mice.
Seven human gastric cancer xenografts with different concentrations of EGF receptor were established in nude mice. The expression of EGF receptor in the tumors was demonstrated by Western blotting with anti-EGF receptor antibody, binding assay with 125I-EGF and immunohistochemistry with anti-EGF receptor antibody. Western blotting revealed EGF receptor doublet bands at molecular masses of 150 KDa and 170 KDa in all of the samples. The concentration of 125I-EGF binding activity in the tumors ranged from 36.0 to 11,000 fmol/mg protein, with a mean of 345 fmol/mg protein. EGF receptor was also demonstrated immunohistochemically on the apical border of the glands and the cell membrane of the tumor cells. There seemed to be a close correlation between the concentration of 125I-EGF binding activity and the doubling time of these tumors in nude mice (gamma = -0.68). However, no definite correlation was observed between EGF ligand binding and histological features of intestinal type or diffuse type. The expression of EGF receptor appears to facilitate the growth of human gastric cancer xenografts in nude mice.
Kiyama
T
T
First Department of Surgery, Nippon Medical School, Tokyo, Japan.
Onda
M
M
Tokunaga
A
A
Fujita
I
I
Okuda
T
T
Mizutani
T
T
Yoshiyuki
T
T
Shimizu
Y
Y
Nishi
K
K
Matsukura
N
N
D000818:Animals; D015153:Blotting, Western; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000737:chemistry / Q000473:pathology*; D014183:Transplantation, Heterologous
D004815:Epidermal Growth Factor; D066246:ErbB Receptors