Subunits of sterol sulphate sulphohydrolase from human placenta microsomes.
A procedure for separation of the catalytic and regulatory subunits of sterol sulphate sulphohydrolase from human placenta microsomes with the use of Concanavalin A-Sepharose chromatography is presented. The Km value for the catalytic subunit with oestrone sulphate is 1.2 x 10(-5) M. The Hill coefficient value h, for the reconstituted enzyme complex is 3, the S0.5 = 0.68 x 10(-3) M and the value of Km is 0.31 x 10(-12) M. The regulatory subunit is trypsin sensitive, while the catalytic one is resistant to trypsin digestion.
Gniot-Szulzycka
J
J
Department of Biochemistry, Mikołaj Kopernik University, Toruń, Poland.
Wojczuk
B
B
D002850:Chromatography, Gel; D006801:Humans; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology*; D010920:Placenta / Q000201:enzymology*; D013429:Sulfatases / Q000737:chemistry / Q000378:metabolism*
D013429:Sulfatases