Artifact formation during transmethylation of lipid peroxides.
By-product formation during base-catalyzed transesterification of lipid peroxides was observed in model experiments. A partially oxidized linoleic acid methyl ester as well as purified hydroxylinoleates served as test compounds. By-products formed during a simulated transmethylation of purified hydroxylinoleates were separated from the main components by means of thin-layer chromatography. The loss attributable to these by-products amounted to 10%. Oxygenated fatty acid derivatives were completely destroyed by acid-catalyzed transmethylation. Catalytic hydrogenation prior to base-catalyzed transmethylation proved to be a simple means to minimize side-product formation. By using this technique the yield of hydroxystearates from a partially autoxidized linoleic acid methyl ester preparation was improved significantly.
Ulberth
F
F
Department of Dairy Research and Bacteriology, Agricultural University, Vienna, Austria.
Kamptner
W
W
D016477:Artifacts; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D008041:Linoleic Acids / Q000737:chemistry; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000737:chemistry; D008745:Methylation; D010084:Oxidation-Reduction
D008041:Linoleic Acids; D008054:Lipid Peroxides; C005575:methyl linoleate; C008285:linoleic acid hydroperoxide