title: Cloning of the 62-kilodalton component of basic transcription factor BTF2.
abstract: Cloning of the mammalian basic transcription factors serves as a major step in understanding the mechanism of transcription initiation. The 62-kilodalton component (p62) of one of these transcription factors, BTF2 was cloned and overexpressed. A monoclonal antibody to this polypeptide inhibited transcription in vitro. Immunoaffinity experiments demonstrated that the 62-kilodalton component is closely associated with the other polypeptides present in the BTF2 factor. Sequence similarity suggests that BTF2 may be the human counterpart of RNA polymerase II initiation factor b from yeast.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes, Centre National de la Recherche Scientifique, Unité 184, Faculté de Médecine, Strasbourg, France.
authors.forename: L
authors.initials: L
authors.lastname: Fischer
authors.forename: M
authors.initials: M
authors.lastname: Gerard
authors.forename: C
authors.initials: C
authors.lastname: Chalut
authors.forename: Y
authors.initials: Y
authors.lastname: Lutz
authors.forename: S
authors.initials: S
authors.lastname: Humbert
authors.forename: M
authors.initials: M
authors.lastname: Kanno
authors.forename: P
authors.initials: P
authors.lastname: Chambon
authors.forename: J M
authors.initials: JM
authors.lastname: Egly
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
chemical_list: D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH