Detection of T lymphocytes in canine tissue embedded in paraffin wax by means of antibody to CD3 antigen.
This report describes the immunocytochemical detection of CD3 antigen by means of a polyclonal antibody in sections of formol-fixed canine lymphoid tissue, embedded in paraffin wax. In all hybrid organs the lymphocytes in the T cell regions showed an intense immune reaction, located particularly in the cytoplasmic membrane and at the cytoplasmic border.
Ferrer
L
L
Department of Animal Pathology, Faculty of Veterinary Science, Autonomous University of Barcelona, Spain.
Fondevila
D
D
Rabanal
R
R
Ramis
A
A
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D017252:CD3 Complex; D004285:Dogs; D007150:Immunohistochemistry; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell