title: Effect of changes in the nucleotide sequence of the P gene of respiratory syncytial virus on the electrophoretic mobility of the P protein.
abstract: A consensus sequence for the P protein gene of the RSN-2 strain of respiratory syncytial (RS) virus was obtained by PCR amplification of cDNA obtained by reverse transcription. This established that the extent of sequence variation between two P genes of strains of antigenic subtype B is similar to that among A strains, confirming the conservation of P genes within subtypes and the divergence of the two antigenic subtypes of RS virus. The P protein of RS virus exhibits anomalous electrophoretic mobility with respect to its molecular weight. In vitro transcription and translation of RSN-2 strain cDNA possessing single point mutations revealed that substitutions involving charged amino acids in the carboxy-terminal region had a marked effect on the electrophoretic mobility of the P protein.
authors.affiliation: Biological Sciences Department, University of Warwick, Coventry, UK.
authors.forename: C
authors.initials: C
authors.lastname: Caravokyri
authors.forename: C R
authors.initials: CR
authors.lastname: Pringle
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D012136:Respiratory Syncytial Viruses / Q000737:chemistry / Q000235:genetics*; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D010750:Phosphoproteins; D014758:Viral Core Proteins; D015678:Viral Structural Proteins