Biochemical and immunological properties of rat recombinant interleukin-2 and interleukin-4.
We have previously described the isolation and sequencing of cDNA clones encoding rat interleukin-2 (IL-2) and interleukin-4 (IL-4). In the present study, we report the generation of stably transfected Chinese hamster ovary (CHO) cell lines which constitutively synthesize and secrete high levels of rat recombinant IL-2 (rIL-2) and IL-4 (rIL-4). The expression of the cytokine cDNA sequences is driven by the human cytomegalovirus promoter/enhancer within the respective pEE6. HCMV-GS vector constructs, following the successful transfection and isolation of methionine sulphoximine (MSX)-resistant CHO cell lines. Analyses of metabolically labelled CHO.rIL-2 and CHO.rIL-4 have been performed, in addition to studies which demonstrate certain biological properties of these recombinant cytokines including T-cell growth factor activity (rIL-2) and the ability to enhance expression of class II major histocompatibility complex (MHC) molecules on spleen cells (rIL-4). The availability of large quantities of these rat recombinant cytokines, conveniently produced by a mammalian cell line, will prove invaluable in future studies into the induction and regulation of immune responses in this species.
McKnight
A J
AJ
MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, U.K.
Classon
B J
BJ
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D007376:Interleukin-2 / Q000737:chemistry* / Q000276:immunology; D015847:Interleukin-4 / Q000737:chemistry* / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000737:chemistry* / Q000276:immunology; D014162:Transfection
D007376:Interleukin-2; D011994:Recombinant Proteins; D015847:Interleukin-4