Abnormal immune adherence and elimination of hepatitis B surface antigen/antibody complexes in patients with AIDS.
C3b-coated immune complexes (IC) adhere to complement receptor 1 (CR1) on human E in the circulation. E from AIDS patients have an acquired low CR1 number. To study immune adherence and IC elimination in AIDS, radiolabeled hepatitis B surface Ag/antibody complexes were injected i.v. in six AIDS patients and in 14 healthy controls. The binding of IC to E was reduced in AIDS patients (mean binding 2 min after injection: 24.9 +/- 13.3%) compared with healthy individuals (63 +/- 3.7%) (p = 0.0005). The low binding correlated directly with the number of CR1/E and to the capacity of these E to bind IC in vitro. During the first 15 min disappearance of IC was faster in AIDS patients than in normal subjects and correlated with CR1 number. Thereafter, elimination was very slow in AIDS patients, which suggested that a fraction of IC might be released back into the circulation similarly to what has been observed for C3b-coated E. When the data were analyzed with a mathematical model allowing for such release to occur, five of six AIDS patients had a high release rate compared with little or no release in normal individuals (p less than 0.001). Thus, low CR1 on E is responsible for defective immune adherence, and might determine abnormal disappearance of IC from the circulation as well.
Madi
N
N
Département de Médecine, Hôpital Cantonal Universitaire, Geneva, Switzerland.
Steiger
G
G
Estreicher
J
J
Schifferli
J A
JA
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000378:metabolism / Q000493:pharmacokinetics*; D006510:Hepatitis B Antibodies / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006801:Humans; D008657:Metabolic Clearance Rate; D011951:Receptors, Complement / Q000378:metabolism; D017463:Receptors, Complement 3b
D000936:Antigen-Antibody Complex; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D011951:Receptors, Complement; D017463:Receptors, Complement 3b