title: Cytosolic phospholipase A2 from U937 cells: size of the functional enzyme by radiation inactivation.
abstract: We have studied the cytosolic phospholipase A2 (cPLA2) of human U937 cells by radiation inactivation in order to characterize the functional form of the native enzyme by a method that was independent of the discrepancies observed by SDS-PAGE and cDNA cloning. The Radiation Inactivation Size of cPLA2 was reproducible and gave a value of 76,800-80,100 daltons. We eluted the active enzyme from polyacrylamide-gradient gel electrophoresis at a molecular weight of 77,000, confirming the irradiation result. We conclude that cPLA2 is active as the monomeric enzyme and is composed of a single major functional domain that is sensitive to irradiation.
authors.affiliation: Merck Frosst Centre for Therapeutic Research, Merck Frosst Canada Inc., Dorval, Québec.
authors.forename: N M
authors.initials: NM
authors.lastname: Tremblay
authors.forename: D
authors.initials: D
authors.lastname: Nicholson
authors.forename: M
authors.initials: M
authors.lastname: Potier
authors.forename: P K
authors.initials: PK
authors.lastname: Weech
mesh_terms: D002478:Cells, Cultured / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008970:Molecular Weight; D010741:Phospholipases A / Q000737:chemistry* / Q000302:isolation & purification; D054467:Phospholipases A2; D011487:Protein Conformation; D011839:Radiation, Ionizing
chemical_list: D010741:Phospholipases A; D054467:Phospholipases A2