Serological study using alpha-N-acetylgalactosaminidase from Acremonium sp.
alpha-N-Acetylgalactosaminidase, produced by Acremonium sp. destroyed blood group A active substance. This enzyme converted human erythrocytes from group A to O(H). The enzyme was ascertained to liberate N-acetylgalactosamine from group A erythrocytes membranes accompanying a decrease in hemagglutination inhibition activity of A erythrocyte-anti-A serum by the erythrocyte membranes. Glycoproteins and glycolipids extracted from A erythrocytes reduced their blood group A activity after the enzyme treatment. The enzyme also worked on Forssman hapten glycolipid.
Izumi
K
K
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
Yamamoto
K
K
Tochikura
T
T
Hirabayashi
Y
Y
D000017:ABO Blood-Group System; D000116:Acetylgalactosamine / Q000378:metabolism; D000164:Acremonium / Q000201:enzymology*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D006017:Glycolipids / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006385:Hemagglutination Inhibition Tests; D006596:Hexosaminidases / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D048809:alpha-N-Acetylgalactosaminidase
D000017:ABO Blood-Group System; D006017:Glycolipids; D006023:Glycoproteins; D006596:Hexosaminidases; C482722:NAGA protein, human; D048809:alpha-N-Acetylgalactosaminidase; D000116:Acetylgalactosamine