Development of an immunoassay using recombinant maltose-binding protein-STa fusions for quantitating antibody responses against STa, the heat-stable enterotoxin of Escherichia coli.
A set of fusion proteins containing heat-stable enterotoxin (STa) and maltose-binding protein were engineered. These molecules were readily purified and used as solid-phase antigens in an enzyme-linked immunosorbent assay to monitor anti-STa responses in mice immunized with a recombinant vaccine composed of STa and the B subunit of heat-labile enterotoxin.
Aitken
R
R
Department of Genetics, University of Leicester, United Kingdom.
Hirst
T R
TR
D018528:ATP-Binding Cassette Transporters; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001427:Bacterial Toxins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000302:isolation & purification; D002352:Carrier Proteins / Q000276:immunology; D004768:Enterotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000276:immunology*; D029968:Escherichia coli Proteins; D005069:Evaluation Studies as Topic; D058619:Maltose-Binding Proteins; D051379:Mice; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins / Q000276:immunology
D018528:ATP-Binding Cassette Transporters; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; C034151:heat stable toxin (E coli); C461599:maltose transport system, E coli