The effect of A23187 on glucose production from methylglyoxal and pyruvate in isolated murine hepatocytes.
1. A23187 increased the glucose production from methylglyoxal in isolated hepatocytes, and maximal stimulation was obtained at 10(-6) M. The effect of A23187 was dependent on the presence of Ca2+. 2. Glucose production from pyruvate (less than 1 mM) in isolated hepatocytes was stimulated by A23187 in the presence of 2.5 mM Ca2+ and was depressed at pyruvate concentrations above 1 mM. Both the virtual Km and the virtual Vmax of glucose production from pyruvate were decreased by A23187.
Kalapos
M P
MP
Semmelweis University, Medical School, First Institute of Biochemistry, Budapest, Hungary.
Riba
P
P
Garzó
T
T
Antoni
F
F
Mandl
J
J
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005943:Gluconeogenesis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011765:Pyruvaldehyde / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D019289:Pyruvic Acid
D011773:Pyruvates; D000001:Calcimycin; D004533:Egtazic Acid; D011765:Pyruvaldehyde; D019289:Pyruvic Acid; D002118:Calcium