title: Determination of serum glucose by isotope dilution mass spectrometry: candidate definitive method.
abstract: We report a rather simple method to determine glucose concentration in serum, using isotope dilution mass spectrometry and [13C6]glucose as internal standard. The procedure involves a single step of sample purification and the conversion of the analyte into its aldononitrile pentaacetate. The between-day and within-day contribution to total variance for a single measurement was determined by assaying Standard Reference Material (SRM) 909 serum. The method was then applied to measurement of glucose concentration in three lyophilized sera: SRM 909 and two other commercially available sera. In the two studies, the concentration of SRM 909 serum was found to be 0.8% above and 0.3% below the reported value (6.25 mmol/L), respectively; the overall coefficient of variation for determinations in all sera ranged from 0.37% to 0.56%. The precision and the accuracy of the method satisfy the requirements for a Definitive Method.
authors.affiliation: Laboratorio Centrale Analisi, Ospedale S. Raffaele, Milan, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Magni
authors.forename: R
authors.initials: R
authors.lastname: Paroni
authors.forename: P A
authors.initials: PA
authors.lastname: Bonini
authors.forename: M G
authors.initials: MG
authors.lastname: Kienle
mesh_terms: D001786:Blood Glucose / Q000032:analysis*; D006801:Humans; D007201:Indicator Dilution Techniques; D013058:Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D011786:Quality Control
chemical_list: D001786:Blood Glucose