title: Acidic calmodulin binding protein, ACAMP-81, is MARCKS protein interacting with synapsin I.
abstract: ACAMP-81 is an acidic calmodulin binding protein with molecular mass of 81 kDa. We report partial amino acid analysis of ACAMP-81 and its interaction with synapsin I. 123 amino acids of ACAMP-81 were determined and the sequence was completely identical with that of MARCKS protein which was thought to be a substrate for calcium/phospholipid dependent protein kinase (PKC). We found ACAMP-81 bound to synapsin I with 125I-labeled ACAMP-81 overlay method. ACAMP-81 bound to the cysteine specific cleaved 51 kDa fragment derived from middle/tail region of synapsin I.
authors.affiliation: Department of Pharmacology, Nagoya University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Mizutani
authors.forename: H
authors.initials: H
authors.lastname: Tokumitsu
authors.forename: H
authors.initials: H
authors.lastname: Hidaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D010446:Peptide Fragments / Q000302:isolation & purification; D010766:Phosphorylation; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D016704:Synapsins / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D002148:Calmodulin-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D010446:Peptide Fragments; D011506:Proteins; D016704:Synapsins; D000076250:Myristoylated Alanine-Rich C Kinase Substrate