Lyophilised bovine serum as a substitute for frozen serum in the cultivation of Babesia bigemina and B bovis.
Lyophilised serum offers significant advantages over frozen serum when it comes to shipping such material over long distances. Babesia bigemina and B bovis were cultured in medium supplemented by either frozen-thawed or lyophilised-rehydrated serum. There were no significant differences between the two types of medium in the growth of parasites and percentage of infected cells during subcultivation for 18 days.
Fish
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L
Division of Parasitology, Kimron Veterinary Institute, Beit-Dagan, Israel.
Pipano
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E
Indrakamhang
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P
D000818:Animals; D001403:Babesia / Q000254:growth & development*; D016793:Babesia bovis / Q000254:growth & development*; D001793:Blood Preservation; D002417:Cattle / Q000097:blood*; D015925:Cryopreservation; D003470:Culture Media; D005260:Female; D005612:Freeze Drying
D003470:Culture Media