Transfer of isolated nuclei into protoplasts of Aspergillus nidulans.
Nuclei were isolated from protoplasts of a haploid auxotrophic Aspergillus nidulans strain. Transformation of protoplasts prepared from a complementary haploid auxotrophic strain with these purified nuclei resulted in both heterokaryotic and diploid colonies. The nutritionally-complementing colonies appeared at a frequency of 5 x 10(-7) to 10(-8).
Vágvölgyi
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C
Department of Microbiology, Attila József University, Szeged, Hungary.
Ferenczy
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L
D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D053652:Nuclear Transfer Techniques; D011523:Protoplasts; D014170:Transformation, Genetic