Requirement for prolactin during cell cycle regulated gene expression in cloned T-lymphocytes.
The neuroendocrine hormone PRL acts as a progression factor during interleukin-2 (IL2) stimulated lymphocyte proliferation. Since the sequential expression of cell cycle regulated genes occurs during this process, we examined the contribution of IL2 and PRL to specific RNA accumulation. Stimulation of the cloned T cell line L2 with IL2 and PRL induced the sequential expression of interferon regulatory factor-1, c-myc, proliferating cell nuclear antigen, thymidine kinase, cyclin B, and histone H3. Stimulation of L2 cells with PRL alone, however, induced only the expression of interferon regulatory factor-1. Depletion of PRL, through the use of an anti-PRL antiserum, inhibited IL2 driven proliferation and the expression of cyclin B and histone H3. These results demonstrate that PRL may regulate T cell proliferation by enhancing the expression of some genes necessary for entry into S-phase.
Clevenger
C V
CV
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia.
Sillman
A L
AL
Hanley-Hyde
J
J
Prystowsky
M B
MB
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002999:Clone Cells; D004261:DNA Replication / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D007106:Immune Sera; D007376:Interleukin-2 / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D011388:Prolactin / Q000276:immunology / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013936:Thymidine / Q000378:metabolism
D007106:Immune Sera; D007376:Interleukin-2; D012333:RNA, Messenger; D011994:Recombinant Proteins; D011388:Prolactin; D013936:Thymidine