Biological inactivation by singlet oxygen: distinguishing O2(1 delta g) and O2(1 sigma g+)
Experiments were performed to determine whether bacterial inactivation in the separated-surface-sensitizer system for singlet oxygen generation is due to O2(1 delta g) or O2(1 sigma g+). The rates of inactivation of Gram-negative Salmonella typhimurium LT-2 and a nonpigmented strain of Gram-positive Sarcina lutea were found to increase linearly with the concentration of 1 delta g. The gas phase lifetime of the inactivating agent was found to be within the range of values expected for the gas phase lifetime of 1 delta g rather than 1 sigma g+. These measurements conclusively demonstrate that bacterial inactivation in this system is due predominantly to 1 delta g. Therefore, studies of bacterial inactivation with this singlet oxygen generating system can be used to assess the role of singlet oxygen in various biological and medically relevant situations.
Midden
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Chemistry Department, Bowling Green State University, OH 43403.
Dahl
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D000097:Acetonitriles; D005663:Furans / Q000737:chemistry; D007700:Kinetics; D008027:Light; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology*; D012395:Rose Bengal; D012486:Salmonella typhimurium / Q000187:drug effects*; D012505:Sarcina / Q000187:drug effects*; D026082:Singlet Oxygen
D000097:Acetonitriles; D005663:Furans; D026082:Singlet Oxygen; D012395:Rose Bengal; C037555:2,5-dimethylfuran; D010100:Oxygen; C032159:acetonitrile