Analysis of URSG-mediated glucose repression of the GAL1 promoter of Saccharomyces cerevisiae.
Repression of GAL1 expression during growth on glucose is mediated in part by cis-acting promoter elements designated URSG. We show that oligonucleotides containing sequences from two regions of URSG confer glucose repression upon a heterologous promoter. Repression caused by URSG is dependent on trans-acting factors of the glucose repression pathway and is independent of orientation or location within a promoter, suggesting that URSG contains binding sites for a glucose-activated repressor protein(s). Genetic analysis identified three apparently novel genes (URR1, URR3 and URR4) that are specifically required for URSG-mediated repression and may encode such repressor proteins. Mutations in the URR genes suppress the defect in URSG derepression caused by a snf1 mutation.
Flick
J S
JS
Department of Genetics, Washington University Medical School, St. Louis, Missouri 63110.
Johnston
M
M
D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D005947:Glucose / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D014158:Transcription, Genetic
D004271:DNA, Fungal; D005947:Glucose