title: Requirement of N-linked glycosylation site in Drosophila rhodopsin.
abstract: In vitro mutagenesis and germline transformation were used to create a Drosophila mutant, delta Asn20, lacking the N-linked glycosylation site near the amino terminus of the major rhodopsin (Asn20-Gly-Ser changed to Ile-Gly-Ser). Low opsin protein levels are detected in delta Asn20 photoreceptors. Electroretinogram responses of mutant flies show that the residual rhodopsin found in this mutant is capable of initiating phototransduction. The organization of rhabdomeres, the photoreceptor organelle containing nearly all of the rhodopsin, is aberrant in the delta Asn20 mutant and undergoes age-dependent deterioration. These results establish that an N-linked glycosylation site, and likely glycosylation itself, plays a critical role in the maturation of Drosophila rhodopsin.
authors.affiliation: Department of Biological Sciences, University of Notre Dame, IN 46556.
authors.forename: J E
authors.initials: JE
authors.lastname: O'Tousa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001216:Asparagine / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004330:Drosophila; D004591:Electrophoresis, Polyacrylamide Gel; D004596:Electroretinography; D005136:Eye Proteins / Q000032:analysis; D015870:Gene Expression; D006031:Glycosylation; D008027:Light; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010786:Photoreceptor Cells / Q000378:metabolism / Q000648:ultrastructure; D012243:Rhodopsin / Q000235:genetics / Q000378:metabolism*; D017299:Rod Opsins; D015398:Signal Transduction
chemical_list: D005136:Eye Proteins; D017299:Rod Opsins; D001216:Asparagine; D012243:Rhodopsin