[HPTLC separation and fluorodensitometric determination of isoquinoline alkaloids in Chelidonine majus L].
The quantitative analysis of isoquinoline alkaloids in Chelidonine majus L. was investigated and a HPTLC method has been established. Using this method, we separated and determined eight isoquinoline alkaloids, i.e. chelidonine, protopine, berberine, coptisine, tetrahydrocoptisine, 6-methoxydihydrochelerythrine, 6-methoxydihydrosanguinarine and dihydrosanguinarine. The HPTLC method developed used one developing system on high performance silica gel plate (10 x 10 cm). After separation of these eight alkaloids, fluorescence derivatization was carried out in situ. The content was determined by fluorescence scanning. TLC fluorescence derivatization, fluorescence enhancement and fluorescence stability have been studied. Experiments showed that this method is simple, fast, highly sensitive and highly selective.
Niu
C Q
CQ
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
He
L Y
LY
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003720:Densitometry / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007546:Isoquinolines / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D007546:Isoquinolines