title: Changes in permeability of dog tracheal epithelium in response to hydrostatic pressure.
abstract: We tested the hypothesis that, in asthma, the airway epithelial damage and leakage of blood proteins into the lumen are the result of edema and raised submucosal hydrostatic pressure. Sheets of dog tracheal epithelium were mounted in Ussing chambers, and the effects of transepithelial hydrostatic pressure differences (delta P) on conductance (G), [3H]mannitol flux (Jman), and fluorescein isothiocyanate-albumin flux (Jalb) were determined. delta P values of 20 cmH2O directed from the mucosal to submucosal side of the tissue (m----s) had no significant effects on G, Jman, Jalb, or tissue ultrastructure. delta Ps----m caused increases in conductance (G) with a maximal effect at approximately 20 cmH2O. delta Ps----m of 20 cmH2O significantly (P less than 0.05) increased G (4.3 +/- 0.6 to 10.6 +/- 1.6 mS/cm2), Jman s----m (18 +/- 5 to 411 +/- 54 nmol.cm-2.h-1), J(alb)s----m (0.3 +/- 0.1 to 6.0 +/- 2.0 micrograms.cm-2.h-1), and J(alb)m----s (0.7 +/- 0.3 to 1.8 +/- 0.4 micrograms.cm-2.h-1). Jman m----s was not affected. On removal of delta P, G and Jman s----m returned to preexposure values, though J(alb)s----m remained slightly elevated at 1.1 +/- 0.3 micrograms.cm-2.h-1. Morphologically, delta Ps----m caused dilation of lateral intercellular spaces, disruption of tight junctions, and submucosal edema. The large increases in s----m fluxes of albumin and mannitol are consistent with bulk flow of fluid toward the lumen via the areas of epithelial damage.
authors.affiliation: Cystic Fibrosis Research Center, University of California, San Francisco 94143.
authors.forename: M
authors.initials: M
authors.lastname: Kondo
authors.forename: W E
authors.initials: WE
authors.lastname: Finkbeiner
authors.forename: J H
authors.initials: JH
authors.lastname: Widdicombe
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005110:Extracellular Space; D006874:Hydrostatic Pressure; D007365:Intercellular Junctions / Q000648:ultrastructure; D008353:Mannitol / Q000493:pharmacokinetics; D008854:Microscopy, Electron; D010539:Permeability; D012709:Serum Albumin / Q000493:pharmacokinetics; D013395:Sucrose / Q000493:pharmacokinetics; D014132:Trachea / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D012709:Serum Albumin; D008353:Mannitol; D013395:Sucrose