Analysis of peripheral blood of pregnant women for the presence of fetal Y chromosome-specific ZFY gene deoxyribonucleic acid sequences.
Deoxyribonucleic acid sequences of human ZFY (zinc-finger-Y) gene, a Y-chromosome-specific gene and candidate for the testis-determining factor, has been identified by an in vitro enzymatic deoxyribonucleic acid amplification method in peripheral blood specimens of women pregnant with male fetuses. This technique permits detection of ZFY gene deoxyribonucleic acid sequences in as few as a single male cell among 1,000,000 female cells. Maternal blood results were confirmed by amplification of ZFY gene deoxyribonucleic acid sequences in chorionic villus cells and by karyotyping in 33 of 36 pregnant women. There was no false-positive male result, and two of the three blood specimens with false-negative results were obtained from pregnant women at a very early gestational age. With properly designed guidelines, this deoxyribonucleic acid amplification method may be an alternative to determine the fetal sex for those pregnancies at risk for X-linked genetic disorders.
Kao
S M
SM
Department of Biochemistry, Chang Gung Medical College, TaoYuan, Taiwan, Republic of China.
Tang
G C
GC
Hsieh
T T
TT
Young
K C
KC
Wang
H C
HC
Pao
C C
CC
D001483:Base Sequence; D015193:Chorionic Villi Sampling; D004247:DNA / Q000235:genetics*; D005260:Female; D005333:Fetus / Q000502:physiology*; D005796:Genes; D006801:Humans; D007621:Karyotyping; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy / Q000097:blood*; D012732:Sex Determination Analysis; D014998:Y Chromosome / Q000502:physiology*
D004247:DNA