Transforming potential of truncated v-myb and stimulation of replication by gag-myb fusion products.
We have previously reported that truncated forms of the v-myb oncogene of avian myeloblastosis virus (AMV) are expressed in transformed chicken embryo fibroblasts (CEF). In this paper, we show that deletion mutants encoding v-myb products altered in either the DNA-binding or the negative regulatory domains are able to induce CEF transformation. In addition, we report that recombinant plasmids expressing gag-myb fusion proteins are maintained as extrachromosomal forms in transfected cells. This observation provides an important clue for a possible role of myb in the DNA replication processes.
Merzak
A
A
Institut Curie, Centre Universitaire, Orsay, France.
Soret
J
J
Martinerie
C
C
Sureau
A
A
Crochet
J
J
Perbal
B
B
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics; D015683:Gene Products, gag; D066298:In Vitro Techniques; D020626:Oncogene Proteins v-myb; D009857:Oncogenes; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D013329:Structure-Activity Relationship
D004268:DNA-Binding Proteins; D015683:Gene Products, gag; D020626:Oncogene Proteins v-myb; D015682:Retroviridae Proteins, Oncogenic