Cell swelling increases bile flow and taurocholate excretion into bile in isolated perfused rat liver.
The effects of aniso-osmotically and amino-acid-induced cell-volume changes on bile flow and biliary taurocholate excretion were studied in isolated perfused rat liver. With taurocholate (100 microM) in the influent perfusate, hypo-osmotic exposure (225 mosmol/l) increased taurocholate excretion into bile and bile flow by 42 and 27% respectively, whereas inhibition by 32 and 47% respectively was observed after hyperosmotic (385 mosmol/l) exposure. The effects of aniso-moticity on taurocholate excretion into bile was observed throughout aniso-osmotic exposure, even after completion of volume-regulatory ion fluxes and were fully reversible upon re-exposure to normo-osmotic media. Hypo-osmotic cell swelling (225 mosmol/l) increased the Vmax. of taurocholate translocation from the sinusoidal compartment into bile about 2-fold. Also, cell swelling induced by glutamine and glycine stimulated both bile flow and biliary taurocholate excretion. There was a close relationship between the aniso-osmotically and amino-acid-induced change of cell volume and taurocholate excretion into bile. The data suggest that liver cell volume plays an important role in regulating bile-acid-dependent bile flow and biliary taurocholate excretion.
Hallbrucker
C
C
Medizinische Universitätsklinik, Freiburg, Federal Republic of Germany.
Lang
F
F
Gerok
W
W
Häussinger
D
D
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001646:Bile / Q000187:drug effects / Q000502:physiology*; D007038:Hypotonic Solutions; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013656:Taurocholic Acid / Q000378:metabolism*
D000596:Amino Acids; D007038:Hypotonic Solutions; D012462:Saline Solution, Hypertonic; D012965:Sodium Chloride; D013656:Taurocholic Acid