Light and electron microscope study of GABA-immunoreactive neurones in lamina III of rat spinal cord.
In order to determine whether different morphological types of neurone in lamina III of rat spinal dorsal horn contain different neurotransmitters, a combined Golgi and immunocytochemical study was performed. Semithin sections through the cell bodies of 52 Golgi-impregnated neurones in this lamina were tested with antisera to GABA and glycine. Thirty of these cells were immunoreactive with anti-GABA antiserum and 25 of these also showed glycine-like immunoreactivity. These cells had dendrites which were oriented along the rostrocaudal axis and occupied lamina III, with some extension into lamina IV and the ventral half of lamina II. Although some of the nonimmunoreactive cells had similar morphology, many of them had dendrites which passed in a dorsal and/or ventral direction and crossed laminar boundaries. Three of the neurones which were immunoreactive with both antisera were examined with the electron microscope. These cells received a variety of synapses including some from axons which resembled low threshold myelinated mechanoreceptive primary afferents. These results indicate that there is a relationship between morphology and function for neurones in lamina III. It is suggested that the inhibitory neurones which contain both GABA and glycine selectively regulate the transmission of information from low threshold mechanoreceptive primary afferents to other dorsal horn neurones.
Powell
J J
JJ
Department of Anatomy, University of Glasgow, Scotland.
Todd
A J
AJ
D000818:Animals; D005998:Glycine / Q000032:analysis; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000737:chemistry / Q000648:ultrastructure*; D009475:Neurons, Afferent / Q000737:chemistry / Q000648:ultrastructure; D010146:Pain / Q000503:physiopathology; D051381:Rats / Q000033:anatomy & histology*; D013116:Spinal Cord / Q000166:cytology*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D005680:gamma-Aminobutyric Acid; D005998:Glycine