title: Synthetic peptide with antiproliferative activity: a short C-terminal fragment of the human interferon alpha-2 molecule.
abstract: The biological activity of six synthetic peptides of the 124-144 region of the human interferon alpha-2 (IFN alpha-2) molecule was studied. Peptides were examined for their ability to inhibit mitogen induced proliferation of human blood cells in vitro. Only the peptide corresponding to the amino acid sequence 124-138 (2438) possessed IFN-like antiproliferative activity. Other tested synthetic peptides did not affect cell proliferation in this experimental system. As with the native IFN alpha-2 molecule, the inhibitory effect of the peptide 2438 was dose-dependent. On simultaneous addition of peptide 2438, antiproliferative activity of IFN alpha-2 was enhanced. Direct cytotoxic effects of synthetic peptide 2438 were not revealed. These results suggest that a synthetic peptide corresponding to the 124-138-amino acid sequence of the human IFN alpha-2 molecule serves as a cytostatic agent.
authors.affiliation: Department of Cellular Physiology and Immunology, Biological Faculty, Moscow State University, U.S.S.R.
authors.forename: A V
authors.initials: AV
authors.lastname: Danilkovich
authors.forename: K V
authors.initials: KV
authors.lastname: Freze
authors.forename: A F
authors.initials: AF
authors.lastname: Shevalier
authors.forename: V V
authors.initials: VV
authors.lastname: Samukov
authors.forename: A F
authors.initials: AF
authors.lastname: Kirkin
authors.forename: M V
authors.initials: MV
authors.lastname: Gusev
mesh_terms: D000595:Amino Acid Sequence; D004305:Dose-Response Relationship, Drug; D006801:Humans; D016898:Interferon-alpha / Q000737:chemistry* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D016898:Interferon-alpha; D010446:Peptide Fragments; D010835:Phytohemagglutinins