Transverse pore gradient gel electrophoresis of lipoprotein [a] using methylenebisacrylamide gradients.
We describe a modification of transverse pore gradient gel electrophoresis in which pore size is regulated by crosslinker proportion (%C) rather than by total monomer concentration (%T). We electrophoresed plasma lipoprotein [a] transversely across linear N,N'-methylenebisacrylamide gradients and measured mobility as a function of %C. This method allows for the simultaneous assessment of pore sizes generated over a wide range of crosslinker proportions.
Smejkal
G B
GB
Cleveland Clinic Foundation Research Institute, Department of Vascular Cell Biology and Atherosclerosis Research, OH 44195.
Hoff
H F
HF
D000178:Acrylamides; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D017270:Lipoprotein(a); D008074:Lipoproteins / Q000032:analysis*; D016062:Porosity
D000178:Acrylamides; D017270:Lipoprotein(a); D008074:Lipoproteins; C021221:N,N'-methylenebisacrylamide