Comparison of a monoclonal antibody fluorescent polarization immunoassay with monoclonal antibody radioimmunoassay for cyclosporin determination in whole blood.
We have compared the whole blood concentrations of parent cyclosporin A (CsA) using monoclonal fluorescence polarization immunoassay (FPIA) and radioimmunoassay (RIA) as well as polyclonal FPIA in kidney, heart, and bone marrow transplant patients (n = 89). A good correlation was found between monoclonal FPIA and monoclonal RIA (r = 0.96) and a slightly worse one between polyclonal and monoclonal FPIA (r = 0.90). The interassay coefficient of variation was satisfactory for all the methods-less than 5% for monoclonal FPIA. The monoclonal FPIA assay with Abbott TDx appears to provide rapid, precise, and accurate measurement of parent CsA. It is therefore useful for therapeutic monitoring of CsA in whole blood in kidney, heart, and bone marrow transplant patients.
Alvarez
J S
JS
Clinical Pharmacology Unit, Hospital Santa Cruz, Liencres-Cantabria, Spain.
Sacristan
J A
JA
Alsar
M J
MJ
D000911:Antibodies, Monoclonal; D016026:Bone Marrow Transplantation / Q000276:immunology; D016572:Cyclosporine / Q000097:blood*; D016231:Fluorescence Polarization Immunoassay; D016027:Heart Transplantation / Q000276:immunology; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D016572:Cyclosporine