Neuronal and glial gamma-aminobutyric acid+ transporters are distinct proteins.
In the central nervous system, two subtypes of sodium- and chloride-coupled GABA transporter exist. One is sensitive to ACHC, the other to beta-alanine. They are thought to be of neuronal and glial origin, respectively. GABA transport in membrane vesicles derived from astroglial cells was found to be sodium- and chloride-dependent, electrogenic and much more sensitive to beta-alanine than to ACHC. Immunoblotting with antibodies directed against a variety of sequences of the ACHC-sensitive transporter indicated that none of these epitopes was shared by the glial transporter.
Mabjeesh
N J
NJ
Department of Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem, Israel.
Frese
M
M
Rauen
T
T
Jeserich
G
G
Kanner
B I
BI
D000596:Amino Acids / Q000494:pharmacology; D000598:Amino Acids, Cyclic; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002478:Cells, Cultured; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D050485:GABA Plasma Membrane Transport Proteins; D015151:Immunoblotting; D007700:Kinetics; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D027361:Organic Anion Transporters; D015091:beta-Alanine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000596:Amino Acids; D000598:Amino Acids, Cyclic; D002352:Carrier Proteins; D003509:Cyclohexanecarboxylic Acids; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D027361:Organic Anion Transporters; D015091:beta-Alanine; C015666:3-aminocyclohexanecarboxylic acid; D005680:gamma-Aminobutyric Acid