title: Binding sites involved in the interaction of actin with the N-terminal region of dystrophin.
abstract: Two actin-binding sites have been identified on human dystrophin by proton NMR spectroscopy of synthetic peptides corresponding to defined regions of the polypeptide sequence. These are Actin-Binding Site 1 (ABS1) located at residues 17-26 and Actin-Binding Site 2 (ABS2) in the region of residues 128-156. Using defined fragments of the actin amino acid sequence, ABS1 has been shown to bind to actin in the region represented by residues 83-117 and ABS2 to the C-terminal region represented by residues 350-375. These dystrophin-binding sites lie on the exposed domain in the actin filament.
authors.affiliation: Department of Physiology, University of Birmingham, UK.
authors.forename: B A
authors.initials: BA
authors.lastname: Levine
authors.forename: A J
authors.initials: AJ
authors.lastname: Moir
authors.forename: V B
authors.initials: VB
authors.lastname: Patchell
authors.forename: S V
authors.initials: SV
authors.lastname: Perry
mesh_terms: D000199:Actins / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D016189:Dystrophin / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D013049:Spectrin / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D000199:Actins; D016189:Dystrophin; D013049:Spectrin