title: Intestinal absorption of fish oil in rats previously adapted to diets containing fish oil or corn oil.
abstract: A study was conducted to evaluate whether the composition of previous dietary fat affects the absorption and composition of lymph obtained after a meal of fish oil. Adult male Sprague-Dawley rats were fed diets containing either corn oil or fish oil (MaxEPA) for 2 weeks. They were then given intraduodenally a bolus of an emulsion of 0.5 ml of fish oil plus 0.5 ml of 20 mM sodium taurocholate. Intestinal lymph was collected from a cannula in the main intestinal lymph trunk for various times after oil administration. Rats proportion of the test dose fo fish oil than those fed corn oil. There was an effect of previous diet on the fatty acid composition of the lymph. Rats fed fish oil had a higher percentage of eicosapentaenoic and docosahexaenoic acids in the lymph lipids than those fed corn oil while those fed corn oil had a higher percentage of linoleic acid. These results rule out decreased intestinal absorption as a mechanism for the hypotriacylglycerolemic effect of dietary fish oils. They also indicate a significant contribution of endogenous lipids to the fatty acids in lymph.
authors.affiliation: Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada.
authors.forename: G R
authors.initials: GR
authors.lastname: Herzberg
authors.forename: G A
authors.initials: GA
authors.lastname: Chernenko
authors.forename: J A
authors.initials: JA
authors.lastname: Barrowman
authors.forename: K T
authors.initials: KT
authors.lastname: Kean
authors.forename: K M
authors.initials: KM
authors.lastname: Keough
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D000818:Animals; D003314:Corn Oil / Q000378:metabolism*; D004041:Dietary Fats / Q000494:pharmacology*; D004281:Docosahexaenoic Acids / Q000032:analysis; D015118:Eicosapentaenoic Acid / Q000032:analysis; D005395:Fish Oils / Q000008:administration & dosage / Q000378:metabolism*; D007408:Intestinal Absorption; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000032:analysis; D008196:Lymph / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004041:Dietary Fats; D005395:Fish Oils; D008041:Linoleic Acids; D004281:Docosahexaenoic Acids; D003314:Corn Oil; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid