title: Fibrin gel limits intra-abdominal adhesion formation.
abstract: Autologous fibrin gel (FG) has recently been reported efficacious in hepatic injury; the effects of fibrin compounds on intra-abdominal adhesion formation is controversial. This study evaluated intra-abdominal adhesion formation in a rabbit devascularization model. Seventeen New Zealand rabbits were anesthetized and laparotomy was done. The uterine horns were abraded to punctate bleeding followed by bilateral uterine devascularization. Treatment consisted of 10 cc saline control (c) or FG applied to the uterine horns. Peritoneal lavage was done at 15 minutes for red blood cell (RBC) analysis. Autopsy was performed at 1 week. Adhesions were graded from grade 0 (no adhesions) to grade III (dense adhesions). Adhesion grading revealed no difference in average adhesion grade between FG and C with small bowel (1.0 +/- 1.3 vs 0.5 +/- 1.0); bladder (2.1 +/- 1.1 vs 2.4 +/- 1.2); or uterus (1.2 +/- vs 2.0 +/- 1.2). Adhesion grade was significantly less in FG compared to C for the colon and the abdominal incision (0.4 +/- 0.5 vs 1.7 +/- 1.1 and 1.2 +/- 1.1 vs 3.0 +/- 1.2; P less than 0.05 by t-test). There were no differences in lavage RBC count between FG and C (13.1 x 106 +/- 4.1 x 10(6) vs 8.7 x 106 +/- 3.2 x 10(6)). Fibrin gel significantly decreased incisional and colonic adhesions and reduced other abdominal adhesion formation by a nonhemostatic dependent mechanism.
authors.affiliation: Department of Surgery, Wayne State University School of Medicine, Detroit, Michigan.
authors.forename: G W
authors.initials: GW
authors.lastname: Chmielewski
authors.forename: J M
authors.initials: JM
authors.lastname: Saxe
authors.forename: S A
authors.initials: SA
authors.lastname: Dulchavsky
authors.forename: L N
authors.initials: LN
authors.lastname: Diebel
authors.forename: J K
authors.initials: JK
authors.lastname: Bailey
mesh_terms: D000287:Administration, Topical; D000818:Animals; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D004906:Erythrocyte Count; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D005782:Gels; D007410:Intestinal Diseases / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D010533:Peritoneal Lavage; D011817:Rabbits; D012720:Severity of Illness Index; D000267:Tissue Adhesions
chemical_list: D015718:Fibrin Tissue Adhesive; D005782:Gels