Localization of the human gene for the type I cyclic GMP-dependent protein kinase to chromosome 10.
We have recently characterized cDNAs and genomic DNA fragments for human type I cGMP-dependent protein kinase (cGK). By probing human x hamster hybrid cell lines with a 1.2-kb intron fragment from the human type I cGK gene, we identified a 5.9-kb BglII restriction fragment and localized it to human chromosome 10. In situ hybridization analyses using 3H-labeled cDNA and genomic DNA probes for the human type I cGK to human metaphase chromosomes supported the somatic cell hybrid data and indicated that the gene (PRKG1B; protein kinase, cGMP-dependent) maps to 10p11.2----q11.2.
Orstavik
S
S
Institute of Medical Biochemistry, Rikshospitalet, University of Oslo, Norway.
Sandberg
M
M
Bérubé
D
D
Natarajan
V
V
Simard
J
J
Walter
U
U
Gagné
R
R
Hansson
V
V
Jahnsen
T
T
D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D011494:Protein Kinases / Q000235:genetics*
D011494:Protein Kinases