Characterization of the glyoxysomal isocitrate lyase genes of Aspergillus nidulans (acuD) and Neurospora crassa (acu-3).
The nucleotide sequences of the genes encoding the acetate-inducible glyoxylate cycle enzyme isocitrate lyase from the ascomycete fungi Aspergillus nidulans (acuD) and Neurospora crassa (acu-3) are presented. The respective A. nidulans and N. crassa genes are interrupted at identical positions by two introns and encode proteins of 538 and 543 amino acids, which have 75% identity. The predicted protein sequences do not demonstrate the C-terminal tripeptide S-K-L that has been implicated in peroxisomal targeting and found in the glyoxysomally located enzyme malate synthase from the same species. However, the protein sequences do exhibit a partial repeat which, in common with malate synthase, is located in regions that are absent from, or non-homologous with, the E. coli enzyme, which is not compartmentalized.
Gainey
L D
LD
Department of Microbiology, University of Reading, Whiteknights, UK.
Connerton
I F
IF
Lewis
E H
EH
Turner
G
G
Ballance
D J
DJ
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D006038:Glyoxylates / Q000378:metabolism*; D007522:Isocitrate Lyase / Q000737:chemistry / Q000235:genetics*; D008292:Malate Synthase / Q000737:chemistry / Q000235:genetics; D008830:Microbodies / Q000201:enzymology*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D004271:DNA, Fungal; D006038:Glyoxylates; D008292:Malate Synthase; D007522:Isocitrate Lyase