title: Differential recognition of secretory vesicles by annexins. European Molecular Biology Organization Course "Advanced Techniques for Studying Secretion".
abstract: The interactions of synexin, calpactin, and p68 (annexins VII, II, and VI) with zymogen granules and adrenal medullary chromaffin granules were compared. Synexin promoted the aggregation and fusion of both types of organelles. Calpactin inhibited the action of synexin on zymogen granules but would aggregate only chromaffin granules. p68 inhibited aggregation of chromaffin granules by calpactin and fusion of zymogen granules by synexin. The results demonstrate that these annexins, all of which bind acidic phospholipids, interact differently with different biological membranes and therefore may play specific and non-overlapping roles in cells where they are co-expressed.
authors.affiliation: Department of Physiology, University College, London, U.K.
authors.forename: C E
authors.initials: CE
authors.lastname: Creutz
authors.forename: S
authors.initials: S
authors.lastname: Moss
authors.forename: J M
authors.initials: JM
authors.lastname: Edwardson
authors.forename: I
authors.initials: I
authors.lastname: Hide
authors.forename: B
authors.initials: B
authors.lastname: Gomperts
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism; D000818:Animals; D017317:Annexin A6; D017310:Annexin A7; D017302:Annexins; D002118:Calcium / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002417:Cattle; D002837:Chromaffin Granules / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000378:metabolism; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D009391:Nephelometry and Turbidimetry; D010179:Pancreas / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D017317:Annexin A6; D017310:Annexin A7; D017302:Annexins; D002135:Calcium-Binding Proteins; D008565:Membrane Proteins; D011506:Proteins; D011994:Recombinant Proteins; D002118:Calcium