title: A 3' transcriptional enhancer within the coding sequence of a yeast gene encoding the common subunit of two multi-enzyme complexes.
abstract: A well-defined set of isogenic yeast strains has been constructed whereby each strain contains a different LPD::lacZ gene fusion integrated at the ura3 locus. These LPD::lacZ fusions differ in the amount of the LPD1 gene (encoding lipoamide dehydrogenase) that is fused to the lacZ reporter. Comparison of the beta-galactosidase activities of each strain during growth on glucose or ethanol revealed that some part of the LPD1 coding region between +13 and +700 is involved in activating gene expression in a carbon source-dependent manner. This activation occurs at the mRNA level, and is not mediated by changes in mRNA stability. Therefore, the LPD1 gene appears to contain a transcriptional enhancer that lies 3' to the transcriptional start site, and which responds to carbon source.
authors.affiliation: Institute of Cell and Molecular Biology, University of Edinburgh, UK.
authors.forename: Z
authors.initials: Z
authors.lastname: Zaman
authors.forename: A J
authors.initials: AJ
authors.lastname: Brown
authors.forename: I W
authors.initials: IW
authors.lastname: Dawes
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D008058:Dihydrolipoamide Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000235:genetics; D005800:Genes, Fungal; D007763:Lac Operon; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D009097:Multienzyme Complexes; D012333:RNA, Messenger; D008058:Dihydrolipoamide Dehydrogenase