Biological activity of recombinant Ricinus communis agglutinin A chain produced in Escherichia coli.
DNA encoding Ricinus communis agglutinin A chain was ligated into the E. coli expression vector pDS 5/3. Induced E. coli 71.18 cells which had been transformed with this plasmid express Ricinus communis agglutinin A chain in a soluble and biologically active form. Recombinant Ricinus communis agglutinin A chain had ribosomal RNA N-glycosidase activity and was approximately 10-fold less active than ricin A chain in a cell-free protein synthesis inhibition assay.
O'Hare
M
M
Department of Biological Sciences, University of Warwick, Coventry, UK.
Roberts
L M
LM
Lord
J M
JM
D002474:Cell-Free System / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D037102:Lectins / Q000235:genetics / Q000378:metabolism*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D037121:Plant Lectins; D010947:Plants, Toxic; D011994:Recombinant Proteins / Q000378:metabolism; D054788:Ribosome Inactivating Proteins; D012270:Ribosomes / Q000378:metabolism; D012278:Ricinus / Q000235:genetics
D037102:Lectins; D037121:Plant Lectins; D011994:Recombinant Proteins; C017707:Ricinus communis agglutinin-1; D009699:N-Glycosyl Hydrolases; D054788:Ribosome Inactivating Proteins