title: Chromosomal localization of a human cDNA containing a DIDS binding domain and demonstrating high homology to yeast omnipotent suppressor 45.
abstract: We recently have identified a full-length cDNA (TB3-1) from a human adenocarcinoma cell line T84 cDNA library that encodes a 47.8-kDa protein. TB3-1 shares identity with the putative yeast translation termination factor omnipotent suppressor 45. Using human-mouse somatic cell panel analysis, a family of sequences with high homology to the TB3-1 cDNA clone were localized to human chromosomes 5, 6, 7, and X. Southern analysis of a panel of mammalian and chicken genomic DNA demonstrates that TB3-1 is well conserved in higher vertebrates.
authors.affiliation: Department of Cell Biology, University of Alabama, Birmingham.
authors.forename: H E
authors.initials: HE
authors.lastname: Grenett
authors.forename: P G
authors.initials: PG
authors.lastname: Eipers
authors.forename: V J
authors.initials: VJ
authors.lastname: Kidd
authors.forename: P
authors.initials: P
authors.lastname: Bounelis
authors.forename: G M
authors.initials: GM
authors.lastname: Fuller
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D002874:Chromosome Mapping; D002877:Chromosomes, Human / Q000737:chemistry*; D002896:Chromosomes, Human, Pair 6 / Q000737:chemistry; D002897:Chromosomes, Human, Pair 7 / Q000737:chemistry; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis*; D016153:Genes, Suppressor; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014960:X Chromosome / Q000737:chemistry
chemical_list: D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D004247:DNA; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid