Interleukin 6-induced differentiation of a human B cell line into IgM-secreting plasma cells is mediated by c-fos.
The role of the protooncogene c-fos in interleukin (IL) 6-induced B cell differentiation was assessed. Treatment of SKW 6.4 cells with IL 6 induced a transient and early stimulation of c-fos sense mRNA expression. The effect appeared within 30 min and returned to basal levels after 2 h. The addition of antisense oligonucleotides to c-fos significantly inhibited IL 6-induced IgM production by SKW 6.4 cells (p less than 0.001), whereas control oligonucleotides had no inhibitory effect. These results indicate that activation of c-fos is involved in IL 6-induced differentiation of SKW 6.4 cells into IgM-secreting cells.
Körholz
D
D
Department of Pediatric Hematology and Oncology, Heinrich-Heine-University Medical Center, Düsseldorf.
Gerdau
S
S
Enczmann
J
J
Zessack
N
N
Burdach
S
S
D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D016762:Genes, fos; D006801:Humans; D007075:Immunoglobulin M / Q000235:genetics*; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000494:pharmacology*; D010950:Plasma Cells / Q000166:cytology*; D016760:Proto-Oncogene Proteins c-fos / Q000502:physiology*; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000235:genetics
D007075:Immunoglobulin M; D015850:Interleukin-6; D016760:Proto-Oncogene Proteins c-fos; D016372:RNA, Antisense; D012333:RNA, Messenger