Soluble proteins delivered to dendritic cells via pH-sensitive liposomes induce primary cytotoxic T lymphocyte responses in vitro.
Effective immunity to many infectious agents, particularly viruses, requires a CD8+ cytotoxic T lymphocyte (CTL) response. Understanding how to achieve CTL induction with soluble proteins is important for vaccine development since such antigens are usually not processed appropriately to induce CTL. In the present report, we have demonstrated that a potent primary CTL response against a soluble protein can be achieved by delivering antigen in pH-sensitive liposomes to dendritic cells (DC) either in vivo or in vitro. Since the pH-sensitive liposome delivery system is efficient and easy to use, the approach promises to be valuable both in the study of basic mechanisms in antigen processing, and as a practical means of immunization.
Nair
S
S
Department of Microbiology, College of Veterinary Medicine, University of Tennessee, Knoxville 37996.
Zhou
F
F
Reddy
R
R
Huang
L
L
Rouse
B T
BT
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D004337:Drug Carriers; D005260:Female; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014407:Tumor Cells, Cultured
D004337:Drug Carriers; D008081:Liposomes; D010047:Ovalbumin