Carboplatin as opposed to cisplatin does not stimulate the expression of the human immunodeficiency virus long terminal repeat sequences.
The recombinant plasmid pBHIV1 carrying the long terminal repeat (LTR) of the human immunodeficiency virus 1 (HIV-1), linked to the chloramphenicol acetyl transferase (CAT) gene, was introduced into human and rat fibroblasts. Stable transfectants resistant to geneticin expressed CAT activity from the HIV-1 LTR. It was found that the cytotoxic drug cis-diammine(1,1-cyclobutanedicarboxylato)platinum(II) (carboplatin) at concentrations from 1 x 10(-6) to 1 x 10(-4) M does not stimulate the expression of CAT from the HIV-1 LTR. These results differ from previous studies with the related drug cis-diamminedichloroplatinum(II) which showed stimulation of gene expression from the HIV-1 LTR and suggest that carboplatin could be used in the treatment of cancer patients with Acquired Immune Deficiency Syndrome.
Zoumpourlis
V
V
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
Kerr
D J
DJ
Spandidos
D A
DA
D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy; D000818:Animals; D016190:Carboplatin / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D002455:Cell Division / Q000187:drug effects; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis*; D002945:Cisplatin / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D016325:HIV Long Terminal Repeat / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D051381:Rats; D014162:Transfection
D016190:Carboplatin; D015500:Chloramphenicol O-Acetyltransferase; D002945:Cisplatin