title: A novel mitogen-inducible gene product related to p50/p105-NF-kappa B participates in transactivation through a kappa B site.
abstract: A Rel-related, mitogen-inducible, kappa B-binding protein has been cloned as an immediate-early activation gene of human peripheral blood T cells. The cDNA has an open reading frame of 900 amino acids capable of encoding a 97-kDa protein. This protein is most similar to the 105-kDa precursor polypeptide of p50-NF-kappa B. Like the 105-kDa precursor, it contains an amino-terminal Rel-related domain of about 300 amino acids and a carboxy-terminal domain containing six full cell cycle or ankyrin repeats. In vitro-translated proteins, truncated downstream of the Rel domain and excluding the repeats, bind kappa B sites. We refer to the kappa B-binding, truncated protein as p50B by analogy with p50-NF-kappa B and to the full-length protein as p97. p50B is able to form heteromeric kappa B-binding complexes with RelB, as well as with p65 and p50, the two subunits of NF-kappa B. Transient-transfection experiments in embryonal carcinoma cells demonstrate a functional cooperation between p50B and RelB or p65 in transactivation of a reporter plasmid dependent on a kappa B site. The data imply the existence of a complex family of NF-kappa B-like transcription factors.
authors.affiliation: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: V
authors.initials: V
authors.lastname: Bours
authors.forename: P R
authors.initials: PR
authors.lastname: Burd
authors.forename: K
authors.initials: K
authors.lastname: Brown
authors.forename: J
authors.initials: J
authors.lastname: Villalobos
authors.forename: S
authors.initials: S
authors.lastname: Park
authors.forename: R P
authors.initials: RP
authors.lastname: Ryseck
authors.forename: R
authors.initials: R
authors.lastname: Bravo
authors.forename: K
authors.initials: K
authors.lastname: Kelly
authors.forename: U
authors.initials: U
authors.lastname: Siebenlist
mesh_terms: D000595:Amino Acid Sequence; D017487:Ankyrins; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D020572:Oncogene Proteins v-rel; D016366:Open Reading Frames / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics; D011233:Precipitin Tests; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D013601:T-Lymphocytes / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D017487:Ankyrins; D001798:Blood Proteins; D008565:Membrane Proteins; D008934:Mitogens; D016328:NF-kappa B; D020572:Oncogene Proteins v-rel; D010750:Phosphoproteins; D011498:Protein Precursors; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; C072386:p50B-p97 protein, human; D011505:Protein-Tyrosine Kinases