Dorsal-ventral patterning in Drosophila: DNA binding of snail protein to the single-minded gene.
The Drosophila snail gene is required for proper mesodermal development. Genetic studies suggest that it functions by repressing adjacent ectodermal gene expression including that of the single-minded (sim) gene. The snail gene encodes a protein with a zinc-finger motif, and here we report that the snail gene product is a sequence-specific DNA binding protein. The snail protein recognizes a 14-base-pair consensus sequence that is found nine times in a 2.8-kilobase sim regulatory region. These results provide evidence for the direct control of sim transcription by snail.
Kasai
Y
Y
Department of Biology, University of California, Los Angeles 90024.
Nambu
J R
JR
Lieberman
P M
PM
Crews
S T
ST
D017456:Adenovirus Early Proteins; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001756:Blastoderm / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000502:physiology; D005796:Genes; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009856:Oncogene Proteins, Viral / Q000235:genetics; D011401:Promoter Regions, Genetic; D016335:Zinc Fingers / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D017456:Adenovirus Early Proteins; D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D009856:Oncogene Proteins, Viral; D001616:beta-Galactosidase