Sensitization primes platelet-activating factor (PAF)-induced accumulation of eosinophils in mouse skin lesions: contribution of cytokines to the response.
We have examined the hypothesis that cytokines mediate the enhanced responsiveness of eosinophils to PAF in sensitized mouse skin. PAF (10 ng per site) resulted in a considerable degree of eosinophil accumulation in ovalbumin (OA)-sensitized mice but not in non-sensitized mice. Intradermal preadministration of cytokines (IL-5, IL-3 and GM-CSF) also significantly enhanced PAF-induced migration of eosinophils in a dose-dependent manner. The relative potency with which these cytokines primed cell migration was IL-5 greater than IL-3 greater than GM-CSF, however, each cytokine alone showed no direct effect. We conclude that the sensitization or the exogenous application of cytokines is capable of augmenting PAF-induced eosinophil migration in mice in vivo, and the cytokines thus elicited by sensitization may contribute to the extensive recruitment of inflammatory cells in allergic diseases.
Yukawa
T
T
Department of Medicine and Clinical Immunology, Dokkyo University School of Medicine, Tochigi, Japan.
Terashi
K
K
Terashi
Y
Y
Arima
M
M
Sagara
H
H
Motojima
S
S
Fukuda
T
T
Makino
S
S
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology*; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D015848:Interleukin-5 / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010972:Platelet Activating Factor / Q000494:pharmacology*; D012867:Skin / Q000166:cytology*
D016207:Cytokines; D007377:Interleukin-3; D015848:Interleukin-5; D010972:Platelet Activating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor