title: Immobilization of immunoglobulin on a quartz surface by BrCN without loss of antigen binding capability.
abstract: BrCN-activated quartz was used for the immobilization of monoclonal immunoglobulins. BrCN-activated quartz keeps its ability to quantitatively bind immunoglobulins for at least 8 h. The surface density of the immobilized immunoglobulins was 100 ng cm-2 and they were found to retain their ability to bind antigen in a molar ratio of 1:2 for not less than three months.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow.
authors.forename: L D
authors.initials: LD
authors.lastname: Krapivinskaya
authors.forename: G B
authors.initials: GB
authors.lastname: Krapivinsky
authors.forename: V L
authors.initials: VL
authors.lastname: Ratner
mesh_terms: D000937:Antigen-Antibody Reactions / Q000276:immunology*; D015374:Biosensing Techniques; D003488:Cyanogen Bromide; D007118:Immunoassay; D007136:Immunoglobulins / Q000737:chemistry*; D011791:Quartz; D013499:Surface Properties
chemical_list: D007136:Immunoglobulins; D011791:Quartz; D003488:Cyanogen Bromide