The K2-type killer toxin- and immunity-encoding region from Saccharomyces cerevisiae: structure and expression in yeast.
The cDNA copies of M2-1, the larger heat-cleavage product of M2 double-stranded (ds) RNA, have been synthesized, cloned, sequenced and expressed in yeast. This sequence, in combination with the known terminal sequence of M2-1 dsRNA, identifies a translation reading frame for a 362-amino-acid protein of 38.7 kDa, similar in size to the one of several protein species produced from M2-1 dsRNA in vitro translation. The expression of this cDNA clone in yeast confers both killer and immunity phenotypes.
Meskauskas
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Institute of Botany, Lithuanian Academy of Sciences, Vilnius.
Citavicius
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D000348:Aflatoxins / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004271:DNA, Fungal; D055646:Killer Factors, Yeast; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000276:immunology; D029701:Saccharomyces cerevisiae Proteins
D000348:Aflatoxins; D003062:Codon; D004271:DNA, Fungal; C073585:K2 killer toxin; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins