title: Participation of 650-kDa protease (20 S proteasome) in starfish oocyte maturation.
abstract: A protease involved in oocyte maturation of a starfish, Asterina pectinifera, was explored. Trypsin-like and chymotrypsin-like activities of the 650-kDa protease in oocyte extract were revealed to increase more than twice under the influence of 1-methyladenine before germinal vesicle breakdown (GVBD) during maturation. The inhibitory potencies of leupeptin and its five analogs against the chymotrypsin-like activity, but not the trypsin-like activity, of this protease was well in accord with those against GVBD (Takagi Sawada et al. (1989). Dev. Biol. 133, 609-612). These results indicate that the chymotrypsin-like activity of the 650-kDa protease (most probably 20 S proteasome) plays a key role in starfish oocyte maturation.
authors.affiliation: Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Japan.
authors.forename: M T
authors.initials: MT
authors.lastname: Sawada
authors.forename: T
authors.initials: T
authors.lastname: Someno
authors.forename: M
authors.initials: M
authors.lastname: Hoshi
authors.forename: H
authors.initials: H
authors.lastname: Sawada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002918:Chymotrypsin / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008297:Male; D008540:Meiosis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000302:isolation & purification / Q000378:metabolism*; D009842:Oligopeptides; D009865:Oocytes / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D046988:Proteasome Endopeptidase Complex; D013045:Species Specificity; D013094:Spermatozoa / Q000201:enzymology; D013215:Starfish; D013379:Substrate Specificity; D014357:Trypsin / Q000378:metabolism
chemical_list: D009097:Multienzyme Complexes; D009842:Oligopeptides; D002918:Chymotrypsin; D014357:Trypsin; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex