title: Detection of drugs in human hair using Abbott ADx, with confirmation by gas chromatography/mass spectrometry (GC/MS).
abstract: The authors suggest use of the fluorescence polarization immunoassay (FPIA) technique in evaluation of chronic drug abuse using human hair. Hair was decontaminated in 5 mL of ethanol for 15 min at 37 degrees C and then incubated in 3 mL of 1M sodium hydroxide (NaOH) for 1 h at 100 degrees C. Afterwards, the aliquots were neutralized and analyzed using Abbott ADx for a negative or positive response for the following drugs: benzodiazepines, barbiturates, antidepressants, opiates, cocaine, amphetamine, and cannabis. All the positive samples were confirmed by gas chromatography/mass spectrometry (GC/MS). Only one false positive was detected (caused by interference of a phenothiazine with the antidepressants kit), clearly demonstrating the capability of ADx for toxicological screening of human hair.
authors.affiliation: Institut de Medicine Legale, Strasbourg, France.
authors.forename: P
authors.initials: P
authors.lastname: Kintz
authors.forename: B
authors.initials: B
authors.lastname: Ludes
authors.forename: P
authors.initials: P
authors.lastname: Mangin
mesh_terms: D005454:Fluorescence Polarization; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry*; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results
chemical_list: D013287:Illicit Drugs