Studies on synthetic pathway of xylose-containing N-linked oligosaccharides deduced from substrate specificities of the processing enzymes in sycamore cells (Acer pseudoplatanus L.).
We measured the activities of alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, beta-1,4-mannosyl-glycoprotein beta-1,2-xylosyltransferase and glycoprotein 3-alpha-L-fucosyltransferase in the Golgi fraction of suspension-cultured cells of sycamore (Acer pseudoplatanus L.) using fluorescence-labelled oligosaccharides as acceptor substrates for these transferase reactions. The structures of the pyridylaminated oligosaccharides produced by these reactions were analyzed by two-dimensional sugar mapping using high-performance liquid chromatography. We demonstrated that (formula; see text) was processed to produce by these in vitro reactions. On the basis of these results, we discuss a biosynthetic pathway for xylose containing N-linked oligosaccharides in plant glycoproteins.
Tezuka
K
K
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Hayashi
M
M
Ishihara
H
H
Akazawa
T
T
Takahashi
N
N
D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D005453:Fluorescence; D005647:Fucosyltransferases / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases; D009844:Oligosaccharides / Q000096:biosynthesis*; D010430:Pentosyltransferases / Q000378:metabolism; D013379:Substrate Specificity; D014197:Trees; D014994:Xylose / Q000378:metabolism*; D000097608:UDP Xylose-Protein Xylosyltransferase
D005647:Fucosyltransferases; D005964:Glucosyltransferases; D006023:Glycoproteins; D017351:N-Acetylglucosaminyltransferases; D009844:Oligosaccharides; D010430:Pentosyltransferases; D014994:Xylose; D000097608:UDP Xylose-Protein Xylosyltransferase; C063057:UDP-N-acetylglucosamine-peptide beta-N-acetylglucosaminyltransferase