title: Isolation of the neuropeptide less than Glu-Trp-Leu-Lys-Gly-Arg-Phe-NH2 (Pol-RFamide II) from the hydromedusa Polyorchis penicillatus.
abstract: Using a radioimmunoassay for the sequence Arg-Phe-NH2 (RFamide), we have isolated the peptide less than Glu-Trp-Leu-Lys-Gly-Arg-Phe-NH2 (Pol-RFamide II) from acetic acid extracts of the hydromedusa Polyorchis penicillatus. This peptide is a neuropeptide and constitutes a peptide family together with less than Glu-Leu-Leu-Gly-Gly-Arg-Phe-NH2 (Pol-RFamide I), the first neuropeptide isolated from Polyorchis, and less than Glu-Gly-Arg-Phe-NH2 (Antho-RFamide), a neuropeptide isolated from sea anemones and sea pansies.
authors.affiliation: Centre for Molecular Neurobiology (ZMNH), University of Hamburg, FRG.
authors.forename: C J
authors.initials: CJ
authors.lastname: Grimmelikhuijzen
authors.forename: K L
authors.initials: KL
authors.lastname: Rinehart
authors.forename: A N
authors.initials: AN
authors.lastname: Spencer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D009842:Oligopeptides / Q000737:chemistry* / Q000302:isolation & purification; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D011863:Radioimmunoassay; D037401:Scyphozoa / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D009479:Neuropeptides; D009842:Oligopeptides; C073612:pyroglutamyl-tryptophyl-leucyl-lysyl-glycyl-arginyl-phenylalaninamide; C058047:arginylphenylalaninamide; D011761:Pyrrolidonecarboxylic Acid