[Digital imaging microscopy for intracellular Ca2+ in cultured single rat vascular smooth muscle cells using fluorescent Ca2+ indicator "fura-2"].
We presented the principle and methodology of digital imaging microscopy for intracellular Ca2+ in cultured single vascular smooth muscle cells of the rat using fluorescent Ca2+ indicator "fura-2". The methods seemed useful for studying the physiological and pathological phenomena in a single smooth muscle cell. Analysis of the spatial and temporal dynamics of intracellular Ca2+ might be crucial for studying the active sites of vasoactive or anesthetic drugs in a vascular smooth muscle cell as well as for understanding pathophysiology of the arterial spasm.
Fujihara
H
H
Department of Anesthesiology, Niigata University School of Medicine.
Fukuda
S
S
Fujiwara
N
N
Taga
K
K
Shimoji
K
K
D000818:Animals; D002118:Calcium / Q000032:analysis*; D016257:Fura-2; D007477:Ions; D008853:Microscopy / Q000379:methods*; D009131:Muscle, Smooth, Vascular / Q000737:chemistry* / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains
D007477:Ions; D002118:Calcium; D016257:Fura-2