Opsonic activity of bovine serum and mammary secretion after Escherichia coli J5 vaccination.
Six pairs of cows were used to determine the effects of immunization with an Escherichia coli (O111:B4) J5 bacterin on in vitro opsonization of a smooth heterologous strain of E. coli. One cow in each pair was either immunized with the vaccine or sham-immunized at drying off, 30 d after drying off, and at calving. Opsonizing bacteria with serum collected from vaccinated cows 21 d after calving resulted in higher mean number of intracellular bacteria per phagocytosing neutrophil than opsonizing bacteria with serum collected from control cows. Phagocytic parameters using serum collected at drying off and calving did not differ between treatment groups. A trend for enhanced opsonic activity of colostrum from vaccinates was noted. Enhanced opsonization by serum from vaccinated cows coincided with higher serum IgM titer to E. coli J5 whole cell antigen compared with controls. Serum IgG titers to E. coli J5 did not differ between groups. Colostrum IgG titers to E. coli J5 were greater at calving in vaccinated than in control cows. Colostrum and milk collected 21 d after calving from vaccinated cows had higher IgM titers to E. coli J5 than did mammary secretions from control cows. Numbers of intracellular bacteria per phagocytizing neutrophil were correlated positively with IgM titers to E. coli J5 in both serum and colostrum.
Hogan
J S
JS
Department of Dairy Science, Ohio State University, Wooster.
Todhunter
D A
DA
Tomita
G M
GM
Smith
K L
KL
Schoenberger
P S
PS
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D002417:Cattle; D003126:Colostrum / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000662:veterinary; D022361:Escherichia coli Vaccines; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007075:Immunoglobulin M / Q000032:analysis / Q000097:blood; D008321:Mammary Glands, Animal / Q000276:immunology*; D008414:Mastitis, Bovine / Q000517:prevention & control; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins / Q000097:blood / Q000276:immunology*; D010587:Phagocytosis; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D022361:Escherichia coli Vaccines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009895:Opsonin Proteins