title: In vitro megakaryocyte colony formation in patients with idiopathic thrombocytopenic purpura: differences between acute and chronic ITP.
abstract: In vitro megakaryocyte colony formation from the bone marrow of patients with acute idiopathic thrombocytopenic purpura (ITP) or chronic ITP was compared using a plasma clot system. The number of megakaryocyte colony-forming units (CFU-Meg) was significantly higher (p less than 0.05) in acute ITP compared to chronic ITP (54.3 +/- 68.4 vs. 12.9 +/- 15.3/10(5) nonadherent mononuclear cells, mean +/- SD), and significantly lower (p less than 0.05) in chronic ITP compared to controls (12.9 +/- 15.3 vs. 22.8 +/- 15.9). A significant correlation was observed between platelet recovery 7 and 30 days after culture, and the number of CFU-Meg (r = 0.49 and 0.45, respectively, p less than 0.05). An inverse correlation was observed between platelet count at the time of culture and the number of Megs per colony (r = -0.48, p less than 0.05). These results indicated a difference between acute and chronic ITP in the ability to promote in vitro Meg colony formation and may suppose a different immune mechanism for thrombocytopenia in these two disorders.
authors.affiliation: Department of Hematology, Hôpital A. Morvan, Brest University, School of Medicine, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Abgrall
authors.forename: N
authors.initials: N
authors.lastname: el-Kassar
authors.forename: C
authors.initials: C
authors.lastname: Berthou
authors.forename: I
authors.initials: I
authors.lastname: Renard
authors.forename: J M
authors.initials: JM
authors.lastname: Cauvin
authors.forename: A
authors.initials: A
authors.lastname: Le Pailleur
authors.forename: C
authors.initials: C
authors.lastname: Autrand
authors.forename: L
authors.initials: L
authors.lastname: Sensebe
authors.forename: G
authors.initials: G
authors.lastname: Guern
authors.forename: P
authors.initials: P
authors.lastname: Zilliken
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000276:immunology*; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology*; D013234:Stem Cells