In vivo recognition of orf virus early transcriptional promoters in a vaccinia virus recombinant.
The 4.4-kb BamHI-E fragment of the orf virus (OV) genome contains three discrete open reading frames designated ORF-pp, ORF-1, and ORF-3, all of which are flanked by vaccinia virus-like early transcriptional control sequences. To determine whether the vaccinia transcriptional machinery would recognize these promoters and faithfully transcribe OV genes in vivo the BamHI-E fragment was inserted into the thymidine kinase (TK) locus of vaccinia virus and the recombinant used in transcription studies. Northern blotting analysis of early RNA isolated from 143B-TK- cells infected with the recombinant virus showed that OV genes were transcribed and that the three transcripts of 0.70-(ORF-pp), 0.48- (ORF1), and 0.75-kb (ORF-3) were the same size as their counterparts in OV-infected cells. Analysis of the 5' end of transcripts by S1 nuclease and primer extension showed that the transcriptional start points (tsp) of ORF-pp, ORF-1, and ORF-3 in the recombinant were identical or within four nucleotides of the tsps of the same ORFs in OV. However, there were quantitative differences. ORF-1 was transcribed more efficiently in recombinant virus-infected cells than in those infected with OV and analysis of the putative promoter, 5'-AAAATTGTAAATGTA, showed that it was similar to the 7.5-kDa early promoter of vaccinia virus. This demonstrates that the transcriptional control sequences of OV genes are recognized by vaccinia virus transcriptional factors but that quantitative differences exist suggesting that the generically different transcriptional factors have different promoter sequence requirements for maximal transcription.
Fleming
S B
SB
Health Research Council of New Zealand, Virus Research Unit, Dunedin.
Mercer
A A
AA
Fraser
K M
KM
Lyttle
D J
DJ
Robinson
A J
AJ
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009923:Orf virus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic; D014616:Vaccinia virus / Q000235:genetics
D004274:DNA, Recombinant; D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral