title: The primary structure of peptide Y (PY) of the spiny dogfish, Squalus acanthias: immunocytochemical localisation and isolation from the pancreas.
abstract: 1. Endocrine cells within islets, exocrine parenchyma and ductal epithelium in the pancreas of the spiny dogfish, Squalus acanthias, were immunostained with an antiserum to the C-terminal region of mammalian neuropeptide Y (NPY). 2. Radioimmunoassay of pancreatic extracts with the same antiserum detected immunoreactivity in the dorsal lobe (338 pmol/g) and ventral lobe (433 pmol/g). Reverse phase HPLC analysis of both extracts resolved a single immunoreactive peptide. 3. The primary structure of the isolated peptide was established as: YPPKPENPGEDAPPEELAKYYSALRHYINLITRQRY.NH2. 4. Peptide Y (PY) from Squalus acanthias is identical in primary structure to an NPY-related peptide isolated from the pancreas of Scyliorhinus canicula and has a 31/36 residue homology with porcine NPY. The 5 substitutions are highly-conservative.
authors.affiliation: Comparative Neuroendocrinology Research Group, School of Biology, Queen's University of Belfast, Northern Ireland.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Pan
authors.forename: C
authors.initials: C
authors.lastname: Shaw
authors.forename: D W
authors.initials: DW
authors.lastname: Halton
authors.forename: L
authors.initials: L
authors.lastname: Thim
authors.forename: C F
authors.initials: CF
authors.lastname: Johnston
authors.forename: K D
authors.initials: KD
authors.lastname: Buchanan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004284:Dogfish / Q000145:classification / Q000235:genetics*; D005399:Fishes / Q000235:genetics; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010179:Pancreas / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D009479:Neuropeptides; C076795:spiny dogfish peptide Y