Activation of prothrombin in the presence of human umbilical-vein endothelial cells.
Addition of Factor Xa, Factor Va and prothrombin to immobilized cultured human umbilical-vein endothelial cells resulted after a time delay in thrombin formation. The prothrombin-converting (prothrombinase) activity, however, was not associated with the cell surface. Rather, perturbation by thrombin, either formed in situ or exogenously added, induced a procoagulant phospholipid surface in the fluid phase, which, in the presence of Factor Xa and Factor Va, enabled the assembly of prothrombinase.
Schoen
P
P
Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Limburg, The Netherlands.
Reutelingsperger
C
C
Lindhout
T
T
D017304:Annexin A5; D002135:Calcium-Binding Proteins / Q000494:pharmacology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000502:physiology*; D015943:Factor Va / Q000378:metabolism; D015951:Factor Xa / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011257:Pregnancy Proteins / Q000494:pharmacology; D011516:Prothrombin / Q000378:metabolism*; D013917:Thrombin / Q000378:metabolism; D013925:Thromboplastin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014471:Umbilical Veins
D017304:Annexin A5; D002135:Calcium-Binding Proteins; D011257:Pregnancy Proteins; D015943:Factor Va; D011516:Prothrombin; D013925:Thromboplastin; D013917:Thrombin; D015951:Factor Xa