White muscle differentiation in the eel (Anguilla anguilla L.): changes in the myosin isoforms pattern and ATPase profile during post-metamorphic development.
Myosin isoforms and their light and heavy chains subunits were studied in the white lateral muscle of the eel during the post metamorphic development, in relation with the myosin ATPase profile. At elver stage VI A1 the myosin isoforms pattern was characterized by at least two isoforms, FM3 and FM2. The fast isomyosin type 1 (FM1) appeared during subsequent development. It increased progressively in correlation with the increase in the level of the light chain LC3f. FM1 became predominant at stage VI A4. At the elver stage VI A1, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed at least two heavy chains, namely type II-1 and II-2. The type II-1 heavy chain disappeared in the yellow eel white muscle, and V8-protease peptide map showed the appearance of a minor heavy chain type II-3 as early as stage VI B. Comparison of myosin heavy chains and myosin isoforms patterns showed the comigration of different myosin isoforms during white muscle development. The myosin ATPase profile was characterized by a uniform pattern as far as stage VI A4. A mosaic aspect in white muscle was observed as early as stage VI B, showing the appearance of small acid labile fibers. This observation suggests that the type II-3 heavy chain is specific to the small fibers.
Chanoine
C
C
Laboratoire de Biologie du Développement, URA CNRS 1188, Université René Descartes, Paris, France.
Guyot-Lenfant
M
M
el Attari
A
A
Saadi
A
A
Gallien
C L
CL
D000251:Adenosine Triphosphatases / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D004524:Eels / Q000378:metabolism / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D007536:Isomerism; D008675:Metamorphosis, Biological / Q000502:physiology*; D009132:Muscles / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D009218:Myosins / Q000032:analysis* / Q000378:metabolism
D000251:Adenosine Triphosphatases; D009218:Myosins