title: Equine leukocyte elastase inhibitor. Primary structure and identification as a thymosin-binding protein.
abstract: The primary structure of an elastase inhibitor located in the cytoplasm of leukocytes obtained from the equine species has been determined. By sequence comparison, the protein was found to be a member of the serpin family with strong identity to plasminogen activator inhibitor-2. In contrast to other serpins this protein contained no carbohydrate and had a blocked amino terminus. Preliminary evidence indicates that the inhibitor has the additional feature of being a thymosin beta 4-binding protein, since this polypeptide was always located in purified preparations of the protein. This suggests a physiological role for cytoplasmic elastase inhibitors in the thymosin beta 4-regulated rearrangement of the cytoskeleton of leukocytes.
authors.affiliation: Institute of Molecular Biology, Jagiellonian University, Cracow, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Dubin
authors.forename: J
authors.initials: J
authors.lastname: Travis
authors.forename: J J
authors.initials: JJ
authors.lastname: Enghild
authors.forename: J
authors.initials: J
authors.lastname: Potempa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006736:Horses; D006801:Humans; D019272:Leukocyte Elastase; D008969:Molecular Sequence Data; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000235:genetics; D011971:Receptors, Immunologic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013947:Thymosin / Q000378:metabolism
chemical_list: D011971:Receptors, Immunologic; C034055:thymosin receptor; D013947:Thymosin; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase