Dual localization of the mitochondrial phospholipase A2: outer membrane contact sites and inner membrane.
Mitochondria were fractionated according to a procedure which allowed to get free outer and inner membrane plus two distinct contact sites between the two membranes. The data indicate that phospholipase A2 is localized in outer membrane contact sites and in inner membrane. The enzyme activity is twice higher in the contact site fraction than in the free membrane. The major fatty acids released are linoleic and docosahexanoic acids.
Levrat
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C
Department of General and Medical Biochemistry, INSERM-CNRS U189, Lyon-Sud Medical School, Oullins, France.
Louisot
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P
D000818:Animals; D005227:Fatty Acids / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D007425:Intracellular Membranes / Q000201:enzymology* / Q000648:ultrastructure; D051379:Mice; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000648:ultrastructure; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D013347:Subcellular Fractions / Q000201:enzymology / Q000648:ultrastructure
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010741:Phospholipases A; D054467:Phospholipases A2