Establishment of a sensitive radioimmunoassay for serum thymic factor with a special reference to extraction procedures.
A radioimmunoassay (RIA) for serum thymic factor (facteur thymique serique) was established. This assay could detect less than 0.5 pg of serum thymic factor without the interference by other thymic hormones, including thymopoietin II and thymosin alpha 1. Both zinc-free and zinc-bound serum thymic factor showed a similar standard curve, indicating that this RIA could detect both forms of serum thymic factor in the same way. We observed that two procedures were required prior to the measurement in order to measure serum thymic factor content in plasmas; the first, the extraction procedure to remove substances interfering with the assay nonspecifically; the second, the storage of the sample with ethylenediamine tetraacetic acid disodium salt to inhibit the proteases in the plasma to degrade serum thymic factor. By employing this assay system, we observed that cord blood plasmas contained significantly higher level of serum thymic factor than did adult blood plasmas.
Mukaida
N
N
Department of Clinical Pathology, Jichi Medical School, Tochigi-Ken, Japan.
Morimoto
T
T
Takanashi
N
N
Kawai
T
T
D000328:Adult; D002850:Chromatography, Gel; D004355:Drug Stability; D005069:Evaluation Studies as Topic; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D013935:Thymic Factor, Circulating / Q000032:analysis* / Q000302:isolation & purification
D013935:Thymic Factor, Circulating