Asparagine-linked sugar chains of erythrocyte membrane glycoproteins from Wiskott-Aldrich syndrome are not impaired.
In both healthy controls and patients with Wiskott-Aldrich syndrome, the main oligosaccharide in asparagine-linked sugar chains of the membrane glycoproteins of erythrocytes was biantennary sugar chain with bisected GlcNAc (Gal2-GlcNAc2-Man3-GlcNAc-GlcNAc-Fuc-GlcNAcOT). Biantennary sugar chain with an alpha-fucosyl residue linked at the proximal GlcNAc was seen but biantennary sugar chain without an alpha-fucosyl residue at the proximal GlcNAc was not detected in each subject. There was no difference in quality and quantity of asparagine-linked sugar chains of erythrocyte membrane glycoproteins between healthy controls and the patients. These results suggest that asparagine-linked sugar chains in membrane glycoproteins of hematopoietic cells may not be impaired in Wiskott-Aldrich syndrome.
Kondo
N
N
Department of Pediatrics, Gifu University School of Medicine, Japan.
Motoyoshi
F
F
Orii
T
T
D000293:Adolescent; D001216:Asparagine / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002648:Child; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood* / Q000737:chemistry; D008969:Molecular Sequence Data; D014923:Wiskott-Aldrich Syndrome / Q000097:blood*
D008562:Membrane Glycoproteins; D001216:Asparagine