title: Interaction of human immunodeficiency virus glycoprotein 160 with CD4 in Jurkat cells increases p56lck autophosphorylation and kinase activity.
abstract: The tyrosine protein kinase p56lck, specifically expressed in lymphoid cells, undergoes modifications of its autophosphorylation and kinase activity when these cells are triggered by mAbs to the T cell determinants. The kinase activity and the autophosphorylation of p56lck were analysed following triggering Jurkat cells with the human immunodeficiency virus (HIV) glycoprotein gp160 which interacts with CD4: both the autophosphorylation and the kinase activity are increased within 1-5 min following addition of gp160, this increase is maximum at 5 min and is followed by a gradual return to the basal level within 2 h. Similar to observations made with anti-CD4 mAbs the increase in kinase activity of p56lck is not associated with changes in the gel mobility nor is it associated with T cell activation. Triggering of T cells with a combination of anti-CD3 mAbs which activate T cells but not p56lck and gp160 greatly potentiated the increase of p56lck autophosphorylation and kinase activity.
authors.affiliation: ICGM, Unité INSERM U332, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Soula
authors.forename: R
authors.initials: R
authors.lastname: Fagard
authors.forename: S
authors.initials: S
authors.lastname: Fischer
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D017252:CD3 Complex; D015704:CD4 Antigens / Q000276:immunology*; D002460:Cell Line; D015686:Gene Products, env / Q000276:immunology*; D019302:HIV Envelope Protein gp160; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000276:immunology; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D011498:Protein Precursors / Q000276:immunology*; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D013997:Time Factors
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D015704:CD4 Antigens; D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D009856:Oncogene Proteins, Viral; D011498:Protein Precursors; D011948:Receptors, Antigen, T-Cell; D011505:Protein-Tyrosine Kinases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)