Nucleotide sequence and transcriptional analysis of the p80 gene of Autographa californica nuclear polyhedrosis virus: a homologue of the Orgyia pseudotsugata nuclear polyhedrosis virus capsid-associated gene.
The 67.2- to 68.5-m.u. region of Autographa californica nuclear polyhedrosis virus was sequenced. A large open reading frame (ORF) was identified in the clockwise direction on the circular genome map which could potentially encode an 80-kDa polypeptide. Analysis of the predicted amino acid sequence of this ORF indicated that it was a homologue of the p87 capsid-associated gene of Orgyia pseudotsugata MNPV with an overall amino acid similarity of 34.3%. A late transcript of 2.1 kb was mapped to this open reading frame. An antisense 3.1-kb transcript partially overlapped the 5' end of the 2.1-kb RNA. Anti-extracellular virus sera reacted with a fusion protein consisting of a portion of the AcMNPV p80 gene fused to the bacterial trpE gene product, suggesting that the AcMNPV p80 gene product was also a component of the virus capsid.
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Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
Carstens
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D000595:Amino Acid Sequence; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D004279:DNA, Viral; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
D036022:Capsid Proteins; D004279:DNA, Viral; D014764:Viral Proteins; C076630:Ac-vp80 protein, Autographa californica nucleopolyhedrovirus