[Effect of physico-chemical properties of polyacrylamide gel media on the growth of Escherichia coli colonies].
We studied the growth of Escherichia coli LE-392 colonies on polyacrylamide gels (PAAG) depending on the physico-chemical properties of the latter, i.e. polymer concentration in the gel, swelling degree, bound water content (fm), spin-lattice relaxation and spin-spin relaxation times of water molecule protons, and modulus of elasticity (G0). S- or R-type colonies formed depending on gel properties; the diametral growth rate of S colonies was 3 times less compared with that on the control agar medium (Tryptose broth). The procedure is proposed for preparation of PAAG which rules out syneresis. Functional relations between the polymer concentrations in uniformly swelling gels and concentrations of copolymers in the reaction mixture, fm and G0 were revealed. The fm and G0 parameters can be used for controlling the quality of PAAG.
Rodin
V B
VB
Bulatova
R F
RF
Domotenko
L V
LV
Inkovskaia
T E
TE
Artiukhin
V I
VI
D000180:Acrylic Resins / Q000737:chemistry*; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D003470:Culture Media; D000099166:Polyacrylamides
Vliianie fiziko-khimicheskikh svoĭstv gelevoĭ osnovy poliakrilamidnykh sred na rost koloniĭ Escherichia coli.