title: The response of rapidly formed adult human endothelial-cell monolayers to shear stress of flow: a comparison of fibronectin-coated Teflon and gelatin-impregnated Dacron grafts.
abstract: Endothelial cells labeled with indium 111-oxine were seeded in supraconfluent densities onto fibronectin-coated expanded polytetrafluoroethylene (ePTFE) and gelatin-impregnated Dacron graft segments. These grafts with rapidly formed endothelial-cell monolayers were then exposed to varying shear stresses at flow rates of 200 and 300 ml/min, using tissue culture medium in an artificial flow circuit. As the loss of radioactivity represented endothelial-cell loss, cell retention was calculated by the ratio of counts recorded at different time points during 2 hours of flow to initial counts. Although initial cell adherence to gelatin-impregnated Dacron graft segments was poor compared to ePTFE, once cells were attached they resisted shear stress of flow better at 200 ml/min and equally well at 300 ml/min. The cell retention on fibronectin-coated ePTFE was 55.4 +/- 12.9% at 200 ml/min and 56.5 +/- 15.2% at 300 ml/min; cell retention for gelatin-impregnated Dacron graft segments was 69.0 +/- 6.0% and 66.5 +/- 5.5%, respectively. Qualitatively scanning electron microscopy of both ePTFE and gelatin-impregnated Dacron graft segments showed patchy coverage of grafts with cells. There was preferential attachment of endothelial cells to the nodes on ePTFE, although on gelatin-impregnated Dacron graft segments, cells conformed to the Dacron fibers at different levels and directions with evidence of bridging in the gaps between individual fibers. This study shows conclusively that rapidly formed endothelial-cell monolayers on ePTFE and gelatin-impregnated Dacron graft segments resisted a shear stress of flow equal to that seen in a femoropopliteal vein graft with significant cell retention at 2 hours.
authors.affiliation: Department of Vascular Surgery, Manchester Royal Infirmary, U.K.
authors.forename: R
authors.initials: R
authors.lastname: Vohra
authors.forename: G J
authors.initials: GJ
authors.lastname: Thomson
authors.forename: H M
authors.initials: HM
authors.lastname: Carr
authors.forename: H
authors.initials: H
authors.lastname: Sharma
authors.forename: M G
authors.initials: MG
authors.lastname: Walker
mesh_terms: D001807:Blood Vessel Prosthesis; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D005353:Fibronectins; D005780:Gelatin; D006801:Humans; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010477:Perfusion; D011093:Polyethylene Terephthalates; D011138:Polytetrafluoroethylene; D013314:Stress, Mechanical; D013997:Time Factors
chemical_list: D005353:Fibronectins; D011093:Polyethylene Terephthalates; D005780:Gelatin; D011138:Polytetrafluoroethylene