title: Cyclic 2',3'-phosphates and nontemplated nucleotides at the 3' end of spliceosomal U6 small nuclear RNA's.
abstract: Spliceosomal U6 small nuclear RNA (U6 RNA) in species as diverse as man, frog, fruitfly, and soybean have at their 3' ends a cyclic 2',3'-phosphate (greater than p) apparently derived from uridylic acid residues that were added post-transcriptionally. The 3' ends of U6 RNA's from various sources may be processed in different ways, or to different extents, depending on the organism or stage of development. The presence of a greater than p terminus on U6 RNA may influence the activity of U6 RNA either directly during splicing or indirectly by ensuring that the RNA has a defined length or proper conformation (or both).
authors.affiliation: Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison 53706.
authors.forename: E
authors.initials: E
authors.lastname: Lund
authors.forename: J E
authors.initials: JE
authors.lastname: Dahlberg
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002107:Caenorhabditis; D002850:Chromatography, Gel; D004331:Drosophila melanogaster; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing / Q000502:physiology; D012342:RNA, Small Nuclear / Q000737:chemistry*; D012440:Saccharomyces; D013025:Glycine max; D016833:Trypanosoma brucei rhodesiense; D014982:Xenopus laevis
chemical_list: D012342:RNA, Small Nuclear