Comparative analysis of syngeneic and allogeneic mixed lymphocyte reaction of 'naturally' activated and resting T cells.
'Naturally' activated (NA) or resting T lymphocytes obtained from the spleen of normal BALB/c mice were compared in their capacity to mount a syngeneic mixed lymphocyte reaction (SMLR). Both T-cell subsets were able to proliferate and secrete IL-3/GM-CSF in SMLR cultures. IL-2 was present in 'resting' T-cell SMLR supernatants, and barely detectable in NA T-cell SMLR supernatants. Both NA and 'resting' T-cell SMLRs were inhibited with anti-class II, anti-CD4, or anti-IL-2R MoAbs. NA T cells exhibited a background proliferative and secretory activity in the absence of syngeneic accessory cells. This autonomous activity was susceptible to anti-CD4, but poorly inhibited with anti-class II MoAbs. Both NA and 'resting' T lymphocytes displayed strong responsiveness to allogeneic stimuli. The analysis of the relative frequency of proliferating cells in the SMLR (BALB/c), or allo-MLR (B10, B10.A, B10.D2) from NA or 'resting' T cells indicated an enrichment for syngeneic reactivity among NA T lymphocytes. The meaning of these results for NA T-cell function and repertoire is discussed.
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Department of Immunobiology, Federal Fluminense University, Niteroi, Brazil.
De Jesus
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Da Nobrega
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D000818:Animals; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008222:Lymphokines / Q000032:analysis / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines