title: Effect of particle lipid content on the structure of insect lipophorins.
abstract: Four distinct subspecies of the major insect lipoprotein, lipophorin, that range in overall lipid content from 20 to 51% of the particle mass, were isolated from the hemolymph or oocytes of the tobacco hornworm, Manduca sexta. Examination of these subspecies by electron microscopy revealed distinctive morphologies. Adult high density lipophorin (HDLp-A) was found to be an approximately spherical particle with a diameter of 15 +/- 1 nm, while HDLp-Wanderer 1 (W1), was more rectangular in shape and had a distinct cleft extending into the particle at one end. In the case of HDLp-Wanderer 2 (W2) the cleft was deeper and wider than that in HDLp-W1. In egg very high density lipophorin (VHDLp-E) the cleft was increased in size to the extent that the particle had an overall crescent-like conformation. Circular dichroism spectroscopy of the three lipophorin subspecies that contain only apolipophorin I and II revealed that only minor differences in the global protein secondary structure occur as the particle lipid content is decreased. The VHDLp-E apolipoproteins are an exception in that, while having the same alpha-helix content as HDLp-W1 and HDLp-W2, they contain less beta-structure and correspondingly more random coil. Limited digestion of the apolipoprotein components of the lipophorin subspecies with trypsin revealed that as the lipid content of the particles decreases the susceptibility of the apolipoprotein to proteolytic degradation increases. Likewise, tryptophan fluorescence quenching experiments demonstrated that the relative exposure of lipophorin apolipoprotein tryptophan residues also increases as the particle lipid content decreases.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Canada.
authors.forename: R O
authors.initials: RO
authors.lastname: Ryan
authors.forename: C M
authors.initials: CM
authors.lastname: Kay
authors.forename: K
authors.initials: K
authors.lastname: Oikawa
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: R
authors.initials: R
authors.lastname: Bradley
authors.forename: D G
authors.initials: DG
authors.lastname: Scraba
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D002942:Circular Dichroism; D008055:Lipids / Q000737:chemistry*; D008074:Lipoproteins; D008854:Microscopy, Electron; D009036:Moths; D013050:Spectrometry, Fluorescence; D014357:Trypsin
chemical_list: D001053:Apolipoproteins; D002352:Carrier Proteins; D008055:Lipids; D008074:Lipoproteins; C032830:lipophorin; D014357:Trypsin