Metabolism of glycerol by mature boar spermatozoa.
Mature boar spermatozoa oxidized glycerol to carbon dioxide in the absence of any detectable activity of glycerol kinase. With triosephosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase inhibited by the presence of 3-chloro-1-hydroxypropanone (CHOP), dihydroxyacetone phosphate accumulated in incubates when glycerol-3-phosphate was the substrate, but not when it was glycerol. Both dihydroxyacetone and glyceraldehyde could be used as substrates; in the presence of CHOP, dihydroxyacetone phosphate and fructose-1,6-bisphosphate accumulated when dihydroxyacetone was the substrate, but not when it was glyceraldehyde. The metabolic pathways glycerol----glyceraldehyde----glyceraldehyde 3-phosphate and dihydroxyacetone----dihydroxyacetone phosphate have been shown to operate in these cells.
Jones
A R
AR
Department of Biochemistry, University of Sydney, NSW, Australia.
Chantrill
L A
LA
Cokinakis
A
A
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D002478:Cells, Cultured; D004098:Dihydroxyacetone / Q000378:metabolism; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D005635:Fructosediphosphates / Q000378:metabolism; D005985:Glyceraldehyde / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D008297:Male; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*
D005635:Fructosediphosphates; D002245:Carbon Dioxide; D005985:Glyceraldehyde; D004099:Dihydroxyacetone Phosphate; C029063:fructose-1,6-diphosphate; D004098:Dihydroxyacetone; D005990:Glycerol