Induction of heme oxygenase by delta 12-prostaglandin J2 in porcine aortic endothelial cells.
delta 12-Prostaglandin (PG)J2 stimulated the synthesis of a 31,000-dalton protein (termed p31) and the induction of cellular heme oxygenase activity in porcine aortic endothelial cells. A good correlation was observed between the time courses and dose dependencies of the induction of p31 synthesis and that of heme oxygenase activity by delta 12-PGJ2. Hemin, a known inducer of heme oxygenase, also induced p31 synthesis as well as heme oxygenase activity in the cells. On two-dimensional gel electrophoresis, p31 induced by delta 12-PGJ2 exhibited an isoelectric point of 5.4, which coincided exactly with that induced by hemin. These results indicate that the p31 induced by delta 12-PGJ2 in porcine aortic endothelial cells is heme oxygenase.
Koizumi
T
T
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Negishi
M
M
Ichikawa
A
A
D000818:Animals; D001011:Aorta; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D004790:Enzyme Induction; D006360:Heat-Shock Proteins; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D006427:Hemin / Q000494:pharmacology; D007525:Isoelectric Focusing; D008970:Molecular Weight; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000494:pharmacology; D011465:Prostaglandins, Synthetic / Q000494:pharmacology*; D014176:Protein Biosynthesis; D013552:Swine
D006360:Heat-Shock Proteins; D011465:Prostaglandins, Synthetic; C037112:9-deoxy-delta-9-prostaglandin D2; D006427:Hemin; D006419:Heme Oxygenase (Decyclizing); D015230:Prostaglandin D2