Quantitation of HIV-1 proviral DNA in cells from cerebrospinal fluid.
The number of proviral copies in the cerebrospinal fluid of 13 patients in various stages of HIV-1 infection was determined using the polymerase chain reaction. HIV-1 proviral DNA was detected at a median value of 1 provirus per 300 cells with a range from 1 per 20 to 1 per 2,400 cells and a mean value of 1 provirus per 570 cells. Interestingly, this indicates a higher proportion of infected cells in cerebrospinal fluid than is reported to be the case for blood mononuclear cells. However, a clear correlation of these findings to the CDC stage or to neurologic complications was not obvious.
Steuler
H
H
Department of Neurology, University of Heidelberg, Germany.
Munzinger
S
S
Wildemann
B
B
Storch-Hagenlocher
B
B
D000163:Acquired Immunodeficiency Syndrome / Q000134:cerebrospinal fluid / Q000235:genetics / Q000382:microbiology; D001483:Base Sequence; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis / Q000134:cerebrospinal fluid* / Q000737:chemistry; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics*; D013347:Subcellular Fractions / Q000382:microbiology
D004279:DNA, Viral