Age-related decrease in omega conotoxin binding to rat cardiac synaptosomes.
A cardiac synaptosomal preparation developed by this laboratory was used to study neuronal calcium channels in aging rat heart. Ca2+ channels were quantified by measuring binding of iodinated omega conotoxin, which is reported to specifically block neuronal Ca2+ channels. We determined the binding of [125I]-omega conotoxin GVIA to a synaptosomal preparation from the hearts of 6- and 24-month-old male Fisher 344 rats. The maximum number of binding sites (Bmax +/- SD, fmol/mg protein) is lower in preparations from 24-month (2.2 +/- 0.6) than from 6-month (3.4 +/- 0.7)-old rats. This decrease in number of binding sites suggests an age-related reduction in the number of neuronal calcium channels. Since calcium is essential for exocytotic release of norepinephrine and is made available intracellularly through neuronal calcium channels, the reduction in neuronal calcium channel number may explain, in part, our previous observations of diminished release of norepinephrine in senescent hearts.
Parikh
A M
AM
Department of Pharmacology, Medical College of Pennsylvania, Philadelphia 19129.
Johnson
M D
MD
Aloyo
V
V
McIlvain
H B
HB
Bhavsar
V
V
Roberts
J
J
D000367:Age Factors; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000378:metabolism*; D011493:Protein Kinase C / Q000502:physiology; D051381:Rats; D011916:Rats, Inbred F344; D013574:Synaptosomes / Q000378:metabolism*; D020866:omega-Conotoxin GVIA
D002121:Calcium Channel Blockers; D010456:Peptides, Cyclic; D020866:omega-Conotoxin GVIA; D011493:Protein Kinase C; D002118:Calcium