Human brain natriuretic peptide-like immunoreactivity in human brain.
The presence of immunoreactive human brain natriuretic peptide in the human brain was studied with a specific radioimmunoassay for human brain natriuretic peptide-32. This assay showed no significant cross-reaction with human alpha atrial natriuretic peptide, porcine brain natriuretic peptide or rat brain natriuretic peptide. Immunoreactive human brain natriuretic peptide was found in all 5 regions of human brain examined (cerebral cortex, thalamus, cerebellum, pons and hypothalamus) (0.6-6.7 pmol/g wet weight, n = 3). These values were comparable to the concentrations of immunoreactive alpha atrial natriuretic peptide in human brain (0.5-10.1 pmol/g wet weight). However, Sephadex G-50 column chromatography showed that the immunoreactive human brain natriuretic peptide in the human brain eluted earlier than synthetic human brain natriuretic peptide-32. These findings suggest that human brain natriuretic peptide is present in the human brain mainly as larger molecular weight forms.
Takahashi
K
K
Second Department of Internal Medicine, Tohoku University School of Medicine, Miyagi, Japan.
Totsune
K
K
Sone
M
M
Ohneda
M
M
Murakami
O
O
Itoi
K
K
Mouri
T
T
D000368:Aged; D009320:Atrial Natriuretic Factor / Q000276:immunology; D001344:Autopsy; D001923:Brain Chemistry; D003429:Cross Reactions; D005260:Female; D006801:Humans; D008297:Male; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D012680:Sensitivity and Specificity; D013045:Species Specificity
D009419:Nerve Tissue Proteins; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor