Assignment of the gene encoding the catalytic subunit C beta of cAMP-dependent protein kinase to the p36 band on chromosome 1.
A cDNA for the human catalytic subunit (C beta) of cAMP-dependent protein kinase (PKA) has been cloned from a testis cDNA library. In the present study, we have determined the chromosomal localization of this gene using a cDNA for C beta as a probe. Southern blot analysis of genomic DNA from human/mouse cell hybrids revealed that the presence or absence of a 20-kb XbaI fragment, which hybridized with the C beta probe, was concordant with the presence of human chromosome 1. In situ hybridization to metaphase chromosome confirmed the somatic cell hybrid data and regionally mapped the C beta gene of PKA to the p36 band on chromosome 1.
Simard
J
J
MRC Group in Molecular Endocrinology, CHUL Research Center and Laval University, Québec, Canada.
Bérubé
D
D
Sandberg
M
M
Grzeschik
K H
KH
Gagné
R
R
Hansson
V
V
Jahnsen
T
T
D000818:Animals; D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D011494:Protein Kinases / Q000235:genetics*
D015342:DNA Probes; D011494:Protein Kinases