title: Minimally modified lipoproteins in diabetes.
abstract: Studies from several laboratories suggest that oxidized LDL may play an important role in atherogenesis. Our group previously showed that treatment of aortic endothelial cells with low levels of MM-LDL caused increased expression of MCP-1, M-CSF, tissue factor, and a monocyte-binding protein. In these studies MM-LDL was produced by storage of native LDL. We now show that cocultures of endothelial and smooth muscle cells can also produce MM-LDL from native LDL. This production of MM-LDL by cells is prevented by preincubating the LDL with probucol or vitamin E. However, addition of antioxidants to MM-LDL did not block its action. In past studies we also showed that endothelial cells exhibit differential sensitivity to the effects of MM-LDL. We report herein that in resistant cells there is no elevation of catalase, glutathione peroxidase, or copper-zinc-dependent SOD. However, manganese-dependent SOD is elevated in resistant cells. Ways in which MM-LDL production may be elevated in poorly controlled diabetics subjects are discussed.
authors.affiliation: Department of Pathology, University of California, Los Angeles 90024.
authors.forename: J A
authors.initials: JA
authors.lastname: Berliner
authors.forename: M
authors.initials: M
authors.lastname: Territo
authors.forename: M
authors.initials: M
authors.lastname: Navab
authors.forename: A
authors.initials: A
authors.lastname: Andalibi
authors.forename: F
authors.initials: F
authors.lastname: Parhami
authors.forename: F
authors.initials: F
authors.lastname: Liao
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Estworthy
authors.forename: A J
authors.initials: AJ
authors.lastname: Lusis
authors.forename: A M
authors.initials: AM
authors.lastname: Fogelman
mesh_terms: D003920:Diabetes Mellitus / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D008077:Lipoproteins, LDL