title: Successful 96-Hr cold-storage preservation of canine pancreas with UW solution containing the thromboxane A2 synthesis inhibitor OKY046.
abstract: Prostanoids, such as prostacyclin (PGI) and thromboxane A2 (TxA), have been recently suggested to play an important role in preservation-induced injury of pancreas grafts. We have previously shown that the TxA synthesis inhibitor OKY046 prevents a decrease of both the PGI/TxA ratio and blood flow in pancreas grafts after 24-hr preservation with Euro-Collins solution. In our present study, we analyzed whether OKY046 added to University of Wisconsin (UW) solution could extend successful cold-storage preservation of segmental canine pancreas grafts, compared with UW alone. We divided 30 dogs into four preservation groups: Group 1, UW solution for 72 hr (n = 7); Group 2, UW solution for 96 hr (n = 8); Group 3, UW solution plus OKY046 (10(-4) M) for 72 hr (n = 7); and Group 4, UW solution plus OKY046 (10(-4) M) for 96 hr (n = 8). After the cold storage period, segmental pancreas auto-transplantation with immediate completion pancreatectomy was done. Preservation was deemed successful if serum glucose less than 150 mg/dl was maintained for at least 5 days. Intravenous glucose tolerance tests and biopsies were done in those dogs with functioning grafts 14 days post-transplant. Successful preservation rates were as follows: Group 1, 57.1%; Group 2, 12.5%; Group 3, 100%; and Group 4, 75%. The mean K values (+/- standard error) were: Group 1, 1.54 +/- 0.13; Group 2, 0.59; Group 3, 1.54 +/- 0.14; and Group 4, 1.59 +/- 0.24 (not statistically different).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, University of Minnesota, Minneapolis 55455.
authors.forename: S
authors.initials: S
authors.lastname: Kin
authors.forename: E
authors.initials: E
authors.lastname: Stephanian
authors.forename: P
authors.initials: P
authors.lastname: Gores
authors.forename: K
authors.initials: K
authors.lastname: Shirabe
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Salazar
authors.forename: K
authors.initials: K
authors.lastname: Brayman
authors.forename: R
authors.initials: R
authors.lastname: Gruessner
authors.forename: D E
authors.initials: DE
authors.lastname: Sutherland
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003080:Cold Temperature; D004285:Dogs; D011464:Epoprostenol / Q000502:physiology; D005260:Female; D005978:Glutathione; D007328:Insulin; D008297:Male; D008689:Methacrylates / Q000494:pharmacology*; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D016035:Pancreas Transplantation; D011887:Raffinose; D012996:Solutions; D013930:Thromboxane-A Synthase / Q000037:antagonists & inhibitors*; D013931:Thromboxanes / Q000502:physiology; D013997:Time Factors
chemical_list: D007328:Insulin; D008689:Methacrylates; D019188:Organ Preservation Solutions; D012996:Solutions; D013931:Thromboxanes; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D011464:Epoprostenol; D013930:Thromboxane-A Synthase; D005978:Glutathione; D000241:Adenosine; C034364:ozagrel; D011887:Raffinose