Binding specificity of lung surfactant protein SP-D for glucosylceramide.
The specificities of the binding of lung surfactant protein SP-D to glycolipids were examined using 125I-labeled SP-D as a probe. When the binding study was performed on TLC plates, SP-D bound exclusively to GlcCer, whereas it failed to bind to GalCer, GM1, GM2, asialo-GM1, asialo-GM2, sulfatide, Forssman antigen, ceramide dihexoside, ceramide trihexoside, globoside, paragloboside or ceramide. Excess native SP-D competed with 125I-SP-D for the binding to GlcCer. Antibody to rat SP-D inhibited 125I-SP-D binding to GlcCer. Ca2+ was absolutely required for the binding of SP-D to GlcCer; Mg2+ failed to substitute for Ca2+. SP-D bound to ceramide monohexoside in glycolipids isolated from rat lung and bronchoalveolar lavage fluids of rats.
Kuroki
Y
Y
Department of Biochemistry, Sapporo Medical College, Japan.
Gasa
S
S
Ogasawara
Y
Y
Shiratori
M
M
Makita
A
A
Akino
T
T
D000818:Animals; D001667:Binding, Competitive; D001923:Brain Chemistry; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002518:Ceramides / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005963:Glucosylceramides / Q000378:metabolism*; D006017:Glycolipids / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000737:chemistry; D008274:Magnesium / Q000494:pharmacology; D037663:Pulmonary Surfactant-Associated Protein D; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D013154:Spleen / Q000737:chemistry; D013661:Tay-Sachs Disease
D002518:Ceramides; D005963:Glucosylceramides; D006017:Glycolipids; D006023:Glycoproteins; D037663:Pulmonary Surfactant-Associated Protein D; D011663:Pulmonary Surfactants; D008274:Magnesium; D002118:Calcium