title: Translation enhancing properties of the 5'-leader of potato virus X genomic RNA.
abstract: The double-stranded DNA copy corresponding to the 5'-nontranslated alpha beta-leader of potato virus X (PVX) genomic RNA (positions -3 to-85 according to AUG initiator) was chemically synthesized and fused to the transcription plasmids containing three different reporter genes: neomycinphosphotransferase type II (NPT II) gene, Bacillus thuringiensis coleopteran-specific toxic protein gene and beta-glucuronidase (GUS) gene. Expression of the reporter genes in vitro and in plant protoplasts (in the case of GUS gene) reveals that the alpha beta-leader of PVX RNA acts as a translation enhancer despite the presence of the upstream vector-derived sequence and irrespective of the length of the spacer sequence preceding the reporter genes.
authors.affiliation: Department of Virology, Moscow State University, USSR.
authors.forename: D A
authors.initials: DA
authors.lastname: Zelenina
authors.forename: O I
authors.initials: OI
authors.lastname: Kulaeva
authors.forename: E V
authors.initials: EV
authors.lastname: Smirnyagina
authors.forename: A G
authors.initials: AG
authors.lastname: Solovyev
authors.forename: N A
authors.initials: NA
authors.lastname: Miroshnichenko
authors.forename: O N
authors.initials: ON
authors.lastname: Fedorkin
authors.forename: N P
authors.initials: NP
authors.lastname: Rodionova
authors.lastname: Morozov SYu
authors.forename: J G
authors.initials: JG
authors.lastname: Atabekov
mesh_terms: D000818:Animals; D001483:Base Sequence; D002474:Cell-Free System; D003001:Cloning, Molecular; D003062:Codon; D004279:DNA, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D011993:Recombinant Fusion Proteins; D011198:Solanum tuberosum / Q000382:microbiology
chemical_list: D003062:Codon; D004279:DNA, Viral; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins