title: Modes of binding of 2'-AMP to RNase T1. A computer modeling study.
abstract: The modes of binding of adenosine 2'-monophosphate (2'-AMP) to the enzyme ribonuclease (RNase) T1 were determined by computer modelling studies. The phosphate moiety of 2'-AMP binds at the primary phosphate binding site. However, adenine can occupy two distinct sites--(1) The primary base binding site where the guanine of 2'-GMP binds and (2) The subsite close to the N1 subsite for the base on the 3'-side of guanine in a guanyl dinucleotide. The minimum energy conformers corresponding to the two modes of binding of 2'-AMP to RNase T1 were found to be of nearly the same energy implying that in solution 2'-AMP binds to the enzyme in both modes. The conformation of the inhibitor and the predicted hydrogen bonding scheme for the RNase T1-2'-AMP complex in the second binding mode (S) agrees well with the reported x-ray crystallographic study. The existence of the first mode of binding explains the experimental observations that RNase T1 catalyses the hydrolysis of phosphodiester bonds adjacent to adenosine at high enzyme concentrations. A comparison of the interactions of 2'-AMP and 2'-GMP with RNase T1 reveals that Glu58 and Asn98 at the phosphate binding site and Glu46 at the base binding site preferentially stabilise the enzyme-2'-GMP complex.
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
authors.forename: P V
authors.initials: PV
authors.lastname: Balaji
authors.forename: W
authors.initials: W
authors.lastname: Saenger
authors.forename: V S
authors.initials: VS
authors.lastname: Rao
mesh_terms: D000249:Adenosine Monophosphate / Q000737:chemistry*; D001216:Asparagine / Q000737:chemistry; D001665:Binding Sites; D003198:Computer Simulation; D005978:Glutathione / Q000737:chemistry; D006147:Guanine / Q000737:chemistry; D006163:Ribonuclease T1 / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000249:Adenosine Monophosphate; D006147:Guanine; D001216:Asparagine; D006163:Ribonuclease T1; D005978:Glutathione