Two anti-parvovirus B 19 IgM capture assays incorporating a mouse monoclonal antibody specific for B 19 viral capsid proteins VP 1 and VP 2.
During an outbreak of parvovirus B 19 in 1989 in Northern Ireland, 7580 blood donors were screened for B 19 antigen. Two units screened positive, one of which was obtained for use as viral antigen. A monoclonal antibody (R92F6) made against this antigen was specific for B 19 capsid proteins VP 1 and VP 2. The monoclonal antibody was used in the development of 2 anti-B 19 IgM capture enzyme assays. These used a conventional substrate (O-phenylene diamine) and a chemiluminescent signal reagent. There was excellent concordance between the 2 assays. A total of 403 patients sera were tested and 65 sera were positive in each assay.
O'Neill
H J
HJ
Regional Virus Laboratory, Royal Victoria Hospital, Belfast, U.K.
Coyle
P V
PV
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000097:blood / Q000276:immunology; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D004196:Disease Outbreaks; D016731:Erythema Infectiosum / Q000453:epidemiology / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology*; D016253:Microscopy, Immunoelectron; D009660:Northern Ireland / Q000453:epidemiology; D016732:Parvovirus B19, Human / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D007075:Immunoglobulin M