title: HIV-associated thrombocytopenia: in vitro megakaryocyte colony formation in 10 patients.
abstract: In vitro bone-marrow megakaryocyte colony formation was studied in 10 patients with HIV-associated thrombocytopenia to investigate the mechanism of thrombocytopenia. Increased colony formation was observed in 3 patients and decreased growth in 7 patients. No relationship was noted between the growth potential of megakaryocyte progenitors and platelet count, number of CD4+ celts, platelet response to azidothymidine, and platelet count 7 days after culture. In all patients, megakaryocyte morphology was abnormal: blebbing of the membrane and abnormal chromatin with separated lobes of nuclei. Further studies are needed to determine if growth potential of megakaryocyte progenitors is useful in understanding the mechanism of thrombocytopenia in HIV-infected individuals.
authors.affiliation: Service d'Hématologie, CHU, Hôpital Augustin-Morvan, Brest, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Abgrall
authors.forename: N
authors.initials: N
authors.lastname: el Kassar
authors.forename: C
authors.initials: C
authors.lastname: Berthou
authors.forename: J M
authors.initials: JM
authors.lastname: Cauvin
authors.forename: I
authors.initials: I
authors.lastname: Renard
authors.forename: C
authors.initials: C
authors.lastname: Autrand
authors.forename: P
authors.initials: P
authors.lastname: Zilliken
authors.forename: L
authors.initials: L
authors.lastname: Sensebe
authors.forename: G
authors.initials: G
authors.lastname: Guern
authors.forename: G
authors.initials: G
authors.lastname: Le Gall
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000150:complications* / Q000503:physiopathology; D000328:Adult; D003114:Colony-Forming Units Assay; D005260:Female; D006412:Hematopoietic Stem Cells; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000473:pathology; D013921:Thrombocytopenia / Q000097:blood / Q000209:etiology* / Q000503:physiopathology