title: Cloning and characterization of a sixth adenylyl cyclase isoform: types V and VI constitute a subgroup within the mammalian adenylyl cyclase family.
abstract: A sixth member of the mammalian adenylyl cyclase family has been isolated from a canine cardiac cDNA library. This isoform is more highly homologous to type V than to the other adenylyl cyclase types; sequence similarity is apparent even in the transmembrane regions where the greatest divergence among the types exists. Type VI mRNA expression is most abundant in heart and brain; however, unlike type V, a low level of expression is also observed in a variety of other tissues examined. Type VI adenylyl cyclase can be stimulated by NaF, guanosine 5'-[gamma-thio]triphosphate, and forskolin but not by Ca2+/calmodulin, whereas it is inhibited by adenosine and its analogues. Comparison of both their structural and biochemical properties suggests that types V and VI constitute a distinct subgroup of the mammalian adenylyl cyclase family.
authors.affiliation: Department of Pharmacology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
authors.forename: S
authors.initials: S
authors.lastname: Katsushika
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Kawabe
authors.forename: R
authors.initials: R
authors.lastname: Nilakantan
authors.forename: N J
authors.initials: NJ
authors.lastname: Halnon
authors.forename: C J
authors.initials: CJ
authors.lastname: Homcy
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D003001:Cloning, Molecular; D004285:Dogs; D015870:Gene Expression; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D014018:Tissue Distribution; D014162:Transfection
chemical_list: D002147:Calmodulin; D012333:RNA, Messenger; D000262:Adenylyl Cyclases; D000241:Adenosine; D002118:Calcium