[Flow cytometric analysis of effects of chemohormonal agents on the cell cycle distribution of MCF-7 cells].
Five anticancer agents (MTX, 5FU, MMC, ADR and VCR) and an antiestrogenic agent (TAM) were investigated to observe the effects on the growth of MCF-7 cells and the changes on the cell cycle distribution of them by flow cytometry. The growth-inhibitory effects of these agents were divided into three manners. The growth rate by ADR was affected in a concentration-dependent manner; MTX in a time-dependent; 5FU, MMC and VCR in both time and concentration-dependent manners. The effects on cell kinetics were divided into three categories. MCF-7 cells were accumulated on S phase by MTX, G0G1 phases by TAM and G2M phases by the other agents. The combined effect of two agents was the most prominent when MTX was first administered and followed by an agent which affected on G2M phases. In the combination of TAM and MMC, the strongest effect was obtained when MMC was first administered and followed by TAM. These results indicated that the combined effect of cell inhibition was obtained on cell kinetic basis by employing the technique of analytical flow cytometry.
Suzuki
M
M
Second Department of Surgery, Fukushima Medical College, Japan.
D000970:Antineoplastic Agents / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects*; D004317:Doxorubicin / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D013629:Tamoxifen; D004317:Doxorubicin; D005472:Fluorouracil; D008727:Methotrexate