TSH is able to induce cell cycle-related gene expression in rat thyroid cell.
Rat thyroid cells in culture (FRTL-5 strain) require thyrotropic hormone (TSH) for growth. TSH alone in serum free medium is able to induce DNA synthesis of FRTL-5 cells. DNA synthesis occurs 18-20 hours following TSH stimulation of quiescent cells. Here we demonstrate that two sets of genes, related to the entry of cells in the S phase, are induced by TSH: 1) immediate early genes, such as c-jun and a gene coding for a zinc-finger protein Xrox 20/Egr2, both having a pattern of expression similar to the c-fos oncogene; 2) early delayed genes such as ornithine decarboxylase (ODC), 2F-1, a gene that shows a strong similarity in aminoacid sequence to a mitochondrial ADP/ATP carrier, and the asparagine synthetase gene (TS11). Furthermore, an increased expression of the histone H3 gene, a typical marker of S phase, has been observed in TSH-treated FRTL-5 cells.
Colletta
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Istituto di Patologia Umana e Medicina Sociale, Facoltà di Medicina e Chirurgia, Chieti, Italy.
Cirafici
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AM
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D005576:Colforsin / Q000494:pharmacology; D016895:Culture Media, Serum-Free; D015870:Gene Expression / Q000187:drug effects*; D006657:Histones / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology*
D016895:Culture Media, Serum-Free; D006657:Histones; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D005576:Colforsin; D013972:Thyrotropin; D013755:Tetradecanoylphorbol Acetate