title: Comparison of intradermal injections of bromodeoxyuridine and tritiated thymidine in the in vivo measurement of epidermal cell turnover time in goats.
abstract: Study of keratinisation defects such as seborrhoea may require investigation of epidermal cell kinetics in clinical cases. For this to be practically useful a safe, reliable and quick method is essential. This study compared epidermal cell kinetics in the goat following the use of intradermal injection of tritiated thymidine ([3H]TdR) and bromodeoxyuridine (BURD) to label S phase nuclei. Turnover time for goat epithelial cells was not significantly different for the two agents; 26.3 +/- 6.0 days for thymidine and 21.8 +/- 5.2 days for BURD. It was concluded that intradermal injection of BURD will be a suitable technique for investigating epidermal cell kinetics in vivo and could have applications in a clinical situation because it is quicker and safer than [3H]TdR.
authors.affiliation: Department of Clinical Veterinary Medicine, Cambridge.
authors.forename: F C
authors.initials: FC
authors.lastname: Casas
authors.forename: A R
authors.initials: AR
authors.lastname: Jefferies
mesh_terms: D000818:Animals; D001345:Autoradiography; D001973:Bromodeoxyuridine / Q000008:administration & dosage*; D000078404:Epidermal Cells; D006041:Goats / Q000502:physiology*; D007150:Immunohistochemistry; D007271:Injections, Intradermal / Q000662:veterinary; D007700:Kinetics; D016196:S Phase; D013936:Thymidine / Q000008:administration & dosage*
chemical_list: D001973:Bromodeoxyuridine; D013936:Thymidine