A suppressive lymphokine derived from Ts clone 13G2 is IL-10.
CD8+ suppressor T cell (Ts) clone 13G2 produced a soluble suppressive lymphokine, termed the T-cell growth inhibitory factor (TGIF), which suppressed the proliferation of type 1 helper T-cell (Th1) clone 3D20 cells. The specific activity of TGIF was concentrated approximately 66,000-fold (3.7 x 10(6) U/mg of protein) by sequential chromatography from the culture supernatant of 13G2 cells. The suppressive activity of the highly purified TGIF toward the proliferation of Th1 cells was neutralized by the anti-IL-10 monoclonal antibody, and was replaced by IL-10. We detected the IL-10 molecule in the highly purified TGIF by using immunoblotting with the anti-IL-10 antibody. A PCR study showed that mRNA of IL-10 was expressed in the 13G2 cells. From these results, we conclude that TGIF produced from CD8+ T-cell clone 13G2 was IL-10. The suppressive activity of the whole supernatant was also completely blocked by the anti-IL-10 antibody, suggesting that IL-10 could play an important role in immune suppression by Ts cells.
Hisatsune
T
T
Department of Agricultural Chemistry, University of Tokyo, Japan.
Minai
Y
Y
Nishisima
K
K
Enomoto
A
A
Moore
K W
KW
Yokota
T
T
Arai
K
K
Kaminogawa
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D016827:CD8 Antigens; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells / Q000276:immunology*; D006131:Growth Inhibitors / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D015151:Immunoblotting; D016753:Interleukin-10 / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology; D008222:Lymphokines / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D006131:Growth Inhibitors; D008222:Lymphokines; D016753:Interleukin-10