title: Z-DNA as a probe for localized supercoiling in vivo.
abstract: Biological processes such as transcription are expected to generate local variations in DNA supercoiling. The existence of localized supercoiling was recently demonstrated in Escherichia coli by using the supercoil-driven B-to-Z transition as a superhelicity probe. This new methodology is described and its extension to other biological systems discussed.
authors.affiliation: Centre de Biophysique Moléculaire, CNRS, Orleans, France.
authors.forename: A R
authors.initials: AR
authors.lastname: Rahmouni
mesh_terms: D015342:DNA Probes; D004278:DNA, Superhelical / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000502:physiology
chemical_list: D015342:DNA Probes; D004278:DNA, Superhelical