title: Inhibition of immunoglobulin (Ig)G-Fc-mediated cytotoxicity by seminal plasma IgG-Fc receptor III antigens.
abstract: OBJECTIVE
  To determine if the presence of soluble immunoglobulin (Ig)G-Fc receptor III (Fc gamma RIII) antigens in human seminal plasma interfere with IgG-Fc-mediated effector functions.
  
  
  METHODS
  An antibody-dependent cellular cytotoxicity (ADCC) assay was used as a model for IgG-Fc-mediated effector functions. Human red blood cells (RBC), labeled with 51Cr were sensitized with rabbit anti-RBC and used as targets for peripheral blood leukocyte (PBL) effector cells. Cytotoxicity was measured by assessing the release of 51Cr from RBC.
  
  
  METHODS
  (1) Seminal plasma was added at different concentrations to the ADCC, and inhibitory effects were measured. (2) The level of seminal plasma interaction in ADCC was studied by comparing ADCC results in the presence and absence of seminal plasma with findings of target and effector cells that had been preincubated with seminal plasma. (3) The role of seminal plasma Fc gamma RIII in inhibiting ADCC was studied by coincubating seminal plasma with monoclonal antibodies (MAs) Leu 11b that block Fc gamma RIII binding sites. Two isotype-matched MA controls were used at identical concentrations.
  
  
  RESULTS
  (1) Seminal plasma dose dependently inhibits ADCC. (2) Seminal plasma inhibition of ADCC occurs at the level of IgG-Fc interaction with effector cell Fc gamma receptors. (3) Inhibitory effects of seminal plasma on ADCC can be specifically blocked by coincubating seminal plasma with MAs Leu 11b that block Fc gamma RIII binding sites.
  
  
  CONCLUSIONS
  Seminal plasma Fc gamma RIII antigens interfere with IgG-Fc-mediated effector functions. This mechanism could play a beneficial role in controlling potentially harmful antipaternal immune responses.
authors.affiliation: Methodist Hospital of Indiana, Indianapolis 46202.
authors.forename: C J
authors.initials: CJ
authors.lastname: Thaler
authors.forename: P R
authors.initials: PR
authors.lastname: McConnachie
authors.forename: J A
authors.initials: JA
authors.lastname: McIntyre
mesh_terms: D000920:Antibody-Dependent Cell Cytotoxicity; D000943:Antigens, Differentiation / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D008954:Models, Biological; D011961:Receptors, Fc / Q000276:immunology*; D017452:Receptors, IgG; D012661:Semen / Q000276:immunology*
chemical_list: D000943:Antigens, Differentiation; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG