Dissociation-enhanced lanthanide fluorescence immunoassay of lipoprotein(a) in serum.
Lipoprotein(a), a human serum lipoprotein structurally related to low-density lipoprotein (LDL), contains in addition to apolipoprotein B (apo B) apolipoprotein(a) [apo(a)], a glycoprotein with a strong homology to plasminogen. Lp(a) is a risk factor for coronary heart disease and ischemic cerebrovascular disease. Several immunological techniques are used to quantify Lp(a) in human serum, including radioimmunoassays, rocket immunoelectrophoresis, and enzyme-linked immunosorbent assays. Only the last method is suitable for large-scale clinical studies. We describe another solid-phase immunoassay, based on the dissociation-enhanced lanthanide fluorescence system Delfia (Wallac Oy), and outline the technical details. A polyclonal antiserum directed against Lp(a) was used as the capture antibody. Two kinds of detection antibodies were applied, a polyclonal antiserum against apo B and the polyclonal antiserum against Lp(a). The results agree excellently with the values estimated by rocket immunoelectrophoresis. This assay is easily established, measures Lp(a) in a wide concentration range, and is suitable for screening large populations.
Jürgens
G
G
Institute of Medical Biochemistry, Karl-Franzens Universität Graz, Austria.
Hermann
A
A
Aktuna
D
D
Petek
W
W
D004797:Enzyme-Linked Immunosorbent Assay; D005063:Europium; D005470:Fluorometry; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D007122:Immunoelectrophoresis; D017270:Lipoprotein(a); D008074:Lipoproteins / Q000097:blood*; D011863:Radioimmunoassay
D017270:Lipoprotein(a); D008074:Lipoproteins; D005063:Europium