[Chemical reactions in double-helical nucleic acids. XIV. Effectiveness of forming phosphodiester bonds between various nucleotide units].
Efficiency of the template-directed condensation of oligonucleotides depends on the nature of the nucleotide units to be joined. Fourteen out of sixteen dinucleotide combinations in double-stranded DNA were examined. Alterations of the reaction efficiency are identical for cyanogen bromide and 1-ethyl-3-(3'-dimethylaminopropyl)carbodiimide coupling reagents. Dependence of the chemical ligation efficiency on the DNA sequence-specific local conformation is discussed.
Merenkova
I P
IP
Dolinnaia
N G
NG
Oretskaia
T S
TS
Sokolova
N I
NI
Shabarova
Z A
ZA
D001483:Base Sequence; D003488:Cyanogen Bromide / Q000737:chemistry; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D013698:Templates, Genetic
D009696:Nucleic Acids; D009841:Oligonucleotides; D003488:Cyanogen Bromide; D005022:Ethyldimethylaminopropyl Carbodiimide
Khimicheskie reaktsii v dvuspiral'nykh nukleinovykh kislotakh. XIV. Effektivnost' obrazovaniia fosfodiéfirnykh sviazeĭ mezhdu razlichnymi nukleotidnymi zven'iami.