title: Effect of islet amyloid polypeptide (IAPP/amylin) on 2-deoxyglucose uptake in mouse pancreatic acini.
abstract: In order to examine the effect of islet amyloid polypeptide (IAPP/amylin), a product of the pancreatic beta cell and a major component of islet amyloid deposits, on exocrine pancreatic function, we studied the effect of rat IAPP amide (IAPP-NH2) on 2-deoxy-D-glucose (2-DG) uptake in isolated mouse pancreatic acini. Mouse pancreatic acini were preincubated for 80 min with various concentrations of IAPP-NH2 (1 nM-1 microM) and [3H]2-DG uptake for 20 min was measured. The full effect of IAPP-NH2 on this function was not immediate but increased linearly with time for up to 80 min of incubation. IAPP-NH2 caused a dose-dependent stimulation of 2-DG uptake by mouse acini; a detectable effect at 10 nM and a maximal effect at 1 microM. In the presence of 1 microM IAPP-NH2, 2-DG uptake increased by 69 +/- 8% above basal (mean +/- SD, n = 6). The results indicate that IAPP-NH2 stimulates glucose uptake in mouse pancreatic acini, and raise the possibility that IAPP-NH2 plays some physiological role in the insuloacinar axis in mouse pancreas.
authors.affiliation: Division of Endocrinology and Metabolism, Jichi Medical School, Tochigi-Ken, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Iwamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Sakuma
authors.forename: I
authors.initials: I
authors.lastname: Shiraishi
authors.forename: G
authors.initials: G
authors.lastname: Inooka
authors.forename: S
authors.initials: S
authors.lastname: Kumakura
authors.forename: T
authors.initials: T
authors.lastname: Awata
authors.forename: T
authors.initials: T
authors.lastname: Kuzuya
mesh_terms: D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003847:Deoxyglucose / Q000378:metabolism*; D066298:In Vitro Techniques; D058228:Islet Amyloid Polypeptide; D007700:Kinetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium
chemical_list: D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D014316:Tritium; D003847:Deoxyglucose