Expression of the HIV-1 Nef protein in the baculovirus system: investigation of anti-Nef antibodies response in human sera and subcellular localization of Nef.
The nef gene of HIV-1 was expressed in insect cells using the eucaryotic baculovirus system. The recombinant Nef protein frequently reacted with seropositive sera of HIV-1 and HIV-2 infected patients. Anti-Nef antibodies in HIV-1 seronegative high risk groups individuals were only occasionally seen. Confocal laser scanning microscopy demonstrated that Nef is present both in the cytoplasm and in the nucleus, indicating that Nef might directly function on gene expression.
Kienzle
N
N
Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Homburg, Federal Republic of Germany.
Enders
M
M
Buck
M
M
Siakkou
H
H
Jahn
S
S
Petzold
G
G
Schneweis
K E
KE
Bachmann
M
M
Müller
W E
WE
Müller-Lantzsch
N
N
D016367:Baculoviridae / Q000235:genetics*; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D015693:Gene Products, nef / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D015754:Genes, nef; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000097:blood; D006679:HIV Seropositivity / Q000097:blood; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D015498:HIV-2 / Q000276:immunology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology; D054311:nef Gene Products, Human Immunodeficiency Virus
D015693:Gene Products, nef; D015483:HIV Antibodies; D011994:Recombinant Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus