title: Phosphorylation of smg p21B/rap1B p21 by cyclic GMP-dependent protein kinase.
abstract: smg p21B/rap1B p21, a member of ras p21-like small GTP-binding protein superfamily, has been shown to be phosphorylated by cyclic AMP-dependent protein kinase (protein kinase A). We show here that this protein was also phosphorylated by cyclic GMP-dependent protein kinase (protein kinase G) in a cell-free system. The same serine residue (Ser179) in the C-terminal region was phosphorylated by both protein kinases G and A. The Km and Vmax values of smg p21B for protein kinase G were 5 x 10(-7) M and 4 x 10(-9) mol/min/mg, and those values for protein kinase A were 1 x 10(-7) M and 3 x 10(-8) mol/min/mg.
authors.affiliation: Department of Biochemistry, Kobe University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
authors.forename: K
authors.initials: K
authors.lastname: Kaibuchi
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: J D
authors.initials: JD
authors.lastname: Corbin
authors.forename: S H
authors.initials: SH
authors.lastname: Francis
authors.forename: Y
authors.initials: Y
authors.lastname: Takai
mesh_terms: D001345:Autoradiography; D002474:Cell-Free System; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D020668:rap GTP-Binding Proteins
chemical_list: D011518:Proto-Oncogene Proteins; D011494:Protein Kinases; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins