title: Regulation of perfused capillary density in canine intestinal mucosa during endotoxemia.
abstract: When O2 delivery (blood flow X arterial O2 content) is reduced, many tissues respond by increasing perfused capillary density. This facilitates the increase in O2 extraction required to maintain tissue O2 consumption in the face of limited O2 supply. In a previous study of isolated canine small intestine (J. Appl. Physiol. 64: 2410-2419, 1988), endotoxin administration was associated with an impaired ability to increase O2 extraction in response to progressive reductions in O2 delivery. The aim of the present study was to determine whether reductions in perfused capillary density occur after endotoxin administration. Fourteen male dogs were anesthetized with chloralose (150 mg/kg iv) and urethan (750 mg/kg iv), and a segment of small intestine was exteriorized through a midline laparotomy. The segment was isolated vascularly, autoperfused, and maintained at body temperature. Escherichia coli endotoxin (5 mg/kg) or sham challenge was administered, and the animals were allowed to stabilize. Blood flow and arterial and gut venous blood O2 contents were measured after 3 h. Perfused vessels were then labeled by injecting colloidal carbon (less than 0.8 microns) through the arterial cannula and clamping the artery and vein as the bolus passed through the tissue. In some of the experiments a second gut segment was successfully obtained within 1 h of the first, yielding a total of 14 gut segments in nine endotoxin animals and nine segments in five control animals. Morphological analysis of capillary surface density in mucosal villi and crypts showed a significantly higher perfused capillary density in control tissue blocks (77.8 +/- 9.2%) than in blocks from endotoxin-treated animals (68.8 +/- 8.0%, P less than 0.04).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Pulmonary and Critical Care Medicine, University of Chicago, Illinois 60637.
authors.forename: R
authors.initials: R
authors.lastname: Drazenovic
authors.forename: R W
authors.initials: RW
authors.lastname: Samsel
authors.forename: M E
authors.initials: ME
authors.lastname: Wylam
authors.forename: C M
authors.initials: CM
authors.lastname: Doerschuk
authors.forename: P T
authors.initials: PT
authors.lastname: Schumacker
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects / Q000502:physiology; D002196:Capillaries / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D004285:Dogs; D004731:Endotoxins / Q000633:toxicity; D007413:Intestinal Mucosa / Q000098:blood supply*; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D010477:Perfusion; D014115:Toxemia / Q000473:pathology* / Q000503:physiopathology
chemical_list: D004731:Endotoxins