[The lethal and mutagenic action of 3H incorporated into the 2' position of the deoxyribose in the DNA of the extracellular phage lambda].
The lethal and mutagenic effects of 3H decay in 2' position of deoxyribose residues in DNA of extracellular lambda phage were studied, [2'-3H]-deoxyadenosine (3H-dA) or [2'-3H]-thymidine (3H-dT) being used as labelled DNA precursors. As estimated by the efficiency of the lethal and mutagenic actions of 3H decay in position 2' was significantly lower than that of the decay in the incorporated 3H-pyrimidines. The genetic effects of 3H decay in 2' position may be attributed to the radiation effect of beta-particles on DNA. In UV-irradiated E. coli cells, with the induced SOS repair, the mutagenic effect of 3H-dA in phage lambda is significantly higher than that of 3H-dT. This is perhaps related to the formation in DNA of AP-sites, resulting from 3H-decay in 2' position, and to the predominant incorporation of adenosine residues opposite to AP-sites during SOS repair.
Konevega
L V
LV
Kalinin
V L
VL
D010582:Bacteriophage lambda / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D003839:Deoxyadenosines / Q000378:metabolism / Q000528:radiation effects; D003855:Deoxyribose / Q000378:metabolism / Q000528:radiation effects*; D004926:Escherichia coli / Q000528:radiation effects; D016296:Mutagenesis / Q000235:genetics / Q000528:radiation effects*; D013014:SOS Response, Genetics / Q000235:genetics / Q000528:radiation effects; D013936:Thymidine / Q000378:metabolism / Q000528:radiation effects; D014316:Tritium / Q000378:metabolism / Q000633:toxicity*; D014466:Ultraviolet Rays
D004279:DNA, Viral; D003839:Deoxyadenosines; D014316:Tritium; D003855:Deoxyribose; D013936:Thymidine
Letal'noe i mutagennoe deĭstvie 3H, inkorporirovannogo v polozhenie 2' dezoksiribozy v DNK vnekletochnogo faga lambda.