title: Crystallization and preliminary X-ray diffraction studies of mitochondrial short-chain delta 3,delta 2-enoyl-CoA isomerase from rat liver.
abstract: Crystals of short-chain delta 3,delta 2-enoyl-CoA isomerase (EC 5.3.3.8) from rat liver mitochondria have been grown using the hanging-drop vapour diffusion technique. The enoyl-CoA isomerase is an auxiliary enzyme in the beta-oxidation pathway of fatty acid metabolism, and catalyzes the isomerization of unsaturated fatty acids to produce the metabolizable delta 2-trans isomer. The crystals belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions a = 47.9, b = 118.4 and c = 164.8 A, and diffract to 3 A.
authors.affiliation: European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: J P
authors.initials: JP
authors.lastname: Zeelen
authors.forename: R A
authors.initials: RA
authors.lastname: Pauptit
authors.forename: R K
authors.initials: RK
authors.lastname: Wierenga
authors.forename: W H
authors.initials: WH
authors.lastname: Kunau
authors.forename: J K
authors.initials: JK
authors.lastname: Hiltunen
mesh_terms: D000818:Animals; D019748:Carbon-Carbon Double Bond Isomerases; D003460:Crystallization; D064006:Dodecenoyl-CoA Isomerase; D007535:Isomerases / Q000737:chemistry* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology*; D015394:Molecular Structure; D051381:Rats; D014961:X-Ray Diffraction
chemical_list: D007535:Isomerases; D019748:Carbon-Carbon Double Bond Isomerases; D064006:Dodecenoyl-CoA Isomerase