title: [Recombinant interleukin-3 expression system in E. coli].
abstract: Plasmid pTOTE2IL3 (III) has been constructed, expressing an artificial human interleukin-3 (hIL3) gene under conditions of the induced protein biosynthesis. Levels of the recombinant protein synthesis have been compared in several E. coli strains containing expression plasmids pTE2IL3 (I) (constitutive biosynthesis) and (III) (induced biosynthesis). Optimal combinations of the expression plasmids and the bacterial strains are of importance. A simple and effective method has been elaborated for isolation, purification and renaturation of the recombinant protein accumulated in inclusion bodies.
authors.forename: S V
authors.initials: SV
authors.lastname: Lutsenko
authors.forename: A I
authors.initials: AI
authors.lastname: Gurevich
authors.forename: L R
authors.initials: LR
authors.lastname: Ptitsyn
authors.forename: L A
authors.initials: LA
authors.lastname: Riazanova
authors.forename: V A
authors.initials: VA
authors.lastname: Smirnov
mesh_terms: D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D007377:Interleukin-3 / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D007377:Interleukin-3; D011994:Recombinant Proteins
vernacular_title: Sistemy ékspressii v E. coli rekombinantnogo interleĭkina-3.