Sequence and characterization of the met-7 gene of Neurospora crassa.
The proteins encoded by the met-7+ and met-3+ genes of Neurospora crassa are required to form a functional cystathionine-gamma-synthase (CGS). The met-7+ gene has been cloned by complementation of a met-7 mutant. The nucleotide sequence of the complementing DNA reveals the presence of a 542-amino acid open reading frame (ORF). Disruption of this ORF abolishes complementation of the met-7 mutation.
Crawford
J M
JM
Department of Genetics, University of Georgia, Athens 30602.
Geever
R F
RF
Asch
D K
DK
Case
M E
ME
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D019757:Carbon-Oxygen Lyases; D003001:Cloning, Molecular; D005816:Genetic Complementation Test; D008190:Lyases / Q000235:genetics*; D008715:Methionine / Q000235:genetics*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics
D008715:Methionine; C022696:O-succinylhomoserine (thiol)-lyase; D008190:Lyases; D019757:Carbon-Oxygen Lyases