The IL-6 signal transducer, gp130: an oncostatin M receptor and affinity converter for the LIF receptor.
Leukemia inhibitory factor (LIF) and interleukin-6 (IL-6) are multifunctional cytokines with many similar activities. LIF is structurally and functionally related to another cytokine, Oncostatin M (OSM), that binds to the high-affinity LIF receptor but not to the low-affinity LIF receptor. A complementary DNA was isolated that encodes the high-affinity converting subunit of the LIF receptor. The converter conferred high-affinity binding of both LIF and OSM when expressed with the low-affinity LIF receptor and is identical to the signal transducing subunit of the IL-6 receptor, gp130. The gp130 subunit alone confers low-affinity binding of OSM when expressed in COS-7 cells. This receptor system resembles the high-affinity receptors for granulocyte-macrophage colony-stimulating factor, IL-3, and IL-5, which share a common subunit.
Gearing
D P
DP
Immunex Research and Development Corporation, Seattle, WA 98101.
Comeau
M R
MR
Friend
D J
DJ
Gimpel
S D
SD
Thut
C J
CJ
McGourty
J
J
Brasher
K K
KK
King
J A
JA
Gillis
S
S
Mosley
B
B
D000818:Animals; D015703:Antigens, CD; D001667:Binding, Competitive; D002461:Cell Line, Transformed; D050822:Cytokine Receptor gp130; D006131:Growth Inhibitors / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D053683:Oncostatin M; D010455:Peptides / Q000378:metabolism*; D011869:Radioligand Assay; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic / Q000378:metabolism*; D053606:Receptors, OSM-LIF; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
D015703:Antigens, CD; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D008562:Membrane Glycoproteins; D010455:Peptides; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic; D053606:Receptors, OSM-LIF; D011994:Recombinant Proteins; D053683:Oncostatin M; D050822:Cytokine Receptor gp130