Tris discriminates between the different alpha-glucosidase activities from extracts of human neutrophils.
In an attempt to detect acid maltase deficiency in neutrophils from patients with type II glycogenosis, without interference from the 'renal' alpha-glucosidase activity present in these cells, we have evaluated the contribution of the renal component in the total activity measured at pH 4.0 in extracts of human neutrophils. The renal contribution is about 13-25% and renal glucosidase appears to be closely related to the enzyme present on the epithelium of small intestine, which is known to be inhibited by Tris. We have used this compound as a selective inhibitor of the renal component of alpha-glucosidase activity measured at pH 4.0 in total extracts of neutrophils. Our results demonstrate that 0.1 mol/L Tris is an inhibitor of the renal alpha-glucosidase present in neutrophils and can be used to reduce the interference from this enzyme in assays of acid maltase.
Ortiz de Apodaca
M A
MA
Laboratorio de Bioquímica, Hospital Virgen de la Salud, Toledo, Spain.
Fernandez
E
E
de la Fuente
G
G
D065089:Glycoside Hydrolase Inhibitors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000097:blood*; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology*; D014325:Tromethamine / Q000494:pharmacology*; D000520:alpha-Glucosidases / Q000097:blood*
D065089:Glycoside Hydrolase Inhibitors; D007527:Isoenzymes; D014325:Tromethamine; D000520:alpha-Glucosidases