An immunoglobulin-like antigen in human cell lines and sera of cancer patients.
Different sera from cancer patients were tested for the presence of C1-GMA, an antigen described by us, which was shared by HeLa-like human cell lines and gastric mucosa. Using the immunodiffusion test, dot-blotting and electrophoresis followed by immunoblotting, we failed to reveal the antigen in the sera of patients. However, an Ig-like 56-60 kD antigen was found in the sera of patients with ovary and stomach carcinoma and lymphoid tumors. The Ig-like antigen was also revealed in 2 out of 5 tested human cell lines of non-leukocyte origin (HEp-2 and E16-B).
Bobrova
T S
TS
Research Institute of Cancerogenesis, All-Union Cancer Research Center, Academy of Medical Sciences, Moscow, CIS.
Chuev YuV
D000230:Adenocarcinoma / Q000097:blood / Q000276:immunology*; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D002417:Cattle; D003429:Cross Reactions; D005753:Gastric Mucosa / Q000276:immunology; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D005779:Immunodiffusion; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D008970:Molecular Weight; D011817:Rabbits; D013274:Stomach Neoplasms / Q000097:blood / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D007143:Immunoglobulin Heavy Chains; D007136:Immunoglobulins; D014357:Trypsin