Differential regulation by phorbol myristate acetate of IFN-gamma and IL-4 expression in anti-CD3 stimulated mouse spleen cells.
Expression of messenger RNA (mRNA) specific for two cytokines representative of both Th1 and Th2 cell subtypes, interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) respectively, was analysed in murine spleen cells stimulated in vitro with an anti-CD3 monoclonal antibody (mAb). This stimulation induced predominantly an IFN-gamma message. In contrast, in the presence of phorbol myristate acetate (PMA), the expression of IFN-gamma was decreased whereas the IL-4 message was dramatically enhanced. These results were confirmed by Polymerase Chain Reaction (PCR) analysis and extended to include another T-cell activator [Concanavalin A (Con A)]. The results suggest the existence of a differential pathway of activation for the Th subpopulations or a differential regulation of the T helper lymphokine gene expression by PMA-induced signals.
Monteyne
P
P
Unit of Experimental Medicine, Université Catholique de Louvain, Brussels, Belgium.
Renauld
J C
JC
Coutelier
J P
JP
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D015152:Blotting, Northern; D017252:CD3 Complex; D015870:Gene Expression / Q000187:drug effects*; D007371:Interferon-gamma / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics*; D008213:Lymphocyte Activation / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D015847:Interleukin-4; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate