Seasonal inhibition of puberty in domestic gilts is overcome by melatonin administered orally, but not by implant.
The ability of exogenous melatonin, applied either orally or by implant, to overcome the seasonal inhibition of puberty in domestic gilts was tested in two experiments. In Expt 1, 24 gilts received two melatonin implants at 126 days of age and again at 161 days and 196 days, while 24 gilts acted as controls. All gilts were slaughtered at a mean age of 223 days. Blood samples were collected by venepuncture from eight gilts in each treatment at 126, 144 and 178 days of age and the plasma was assayed for melatonin concentration by direct radioimmunoassay. In Expt 2A, four gilts (125 days of age) were fed either 0, 1, 2 or 4 mg of melatonin at 14:00 h on each of four consecutive days. Blood samples for melatonin assay were collected via indwelling jugular catheters every 30 or 60 min from 12:00 to 22:00 h. In Expt 2B, 27 gilts were fed 1 mg of melatonin at 15:00 h each day from 129 days of age until slaughter at 221 days, while 25 gilts acted as controls. In both experiments, the presence of morphologically normal corpora lutea at slaughter was the criterion for puberty. In Expt 1, constant-release melatonin implants had no effect on the percentage of gilts which reached puberty. Among the 24 control gilts, two (8.3%) reached puberty compared with one of the 24 (4.2%) gilts with implants. In all the samples from control gilts, and in the samples taken from treated gilts prior to implantation at 126 days of age, mean plasma melatonin concentration was below the sensitivity of the assay (3.6 pg/ml).(ABSTRACT TRUNCATED AT 250 WORDS)
Paterson
A M
AM
Animal Production Division, Department of Agriculture, South Perth, Australia.
Maxwell
C A
CA
Foldes
A
A
D000284:Administration, Oral; D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D004343:Drug Implants; D005260:Female; D008550:Melatonin / Q000008:administration & dosage* / Q000097:blood; D011863:Radioimmunoassay; D012621:Seasons; D012741:Sexual Maturation / Q000187:drug effects*; D013552:Swine / Q000097:blood / Q000502:physiology*
D004343:Drug Implants; D008550:Melatonin