A comparative study on the electrophoretic patterns of snake venoms.
1. Examination of the polyacrylamide gel electrophoretic (PAGE) and SDS-PAGE patterns of snake venoms shows that these patterns are useful for species differentiation (and hence identification) for snakes of certain genera but have only limited application for snakes from some other genera, due either to the marked individual variations in the venoms or the lack of marked interspecific differences within the same genus. 2. There is no substantial intersubspecific difference in the electrophoretic patterns of the venoms. 3. In general there are no common characteristics in the electrophoretic patterns of the venom at the generic level because of the wide variations in the electrophoretic patterns of venoms of snakes within the same genus. 4. At the familial level, the venoms of Elapidae exhibited SDS-PAGE patterns distinct from those of Crotalidae.
Tan
N H
NH
Department of Biochemistry, University of Malaya, Kuala Lumpur, Malaysia.
Ponnudurai
G
G
D000818:Animals; D003435:Crotalid Venoms / Q000145:classification / Q000302:isolation & purification; D004546:Elapid Venoms / Q000145:classification / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D012910:Snake Venoms / Q000145:classification / Q000302:isolation & purification*; D012911:Snakes / Q000145:classification; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity; D014757:Viper Venoms / Q000145:classification / Q000302:isolation & purification
D003435:Crotalid Venoms; D004546:Elapid Venoms; D012910:Snake Venoms; D014757:Viper Venoms; D012967:Sodium Dodecyl Sulfate