title: Cross-linkage and cross-linking of peptidoglycan in Escherichia coli: definition, determination, and implications.
abstract: The glycan chains in peptidoglycan or murein are cross-linked by transpeptidation of the peptide side chains. To assess the fraction of side chains involved in cross-bridges, distinction has been made between cross-linkage and cross-linking. The first expression refers to the situation in unlabeled (or fully labeled) peptidoglycan, and the second refers to pulse-labeled peptidoglycan. It is argued that for the determination of the cross-linking value, the mode of insertion as denoted by the so-called acceptor/donor radioactivity ratio should be taken into account.
authors.affiliation: Department of Microbiology, University of Amsterdam, The Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: Driehuis
authors.forename: B
authors.initials: B
authors.lastname: de Jonge
authors.forename: N
authors.initials: N
authors.lastname: Nanninga
mesh_terms: D003432:Cross-Linking Reagents; D004926:Escherichia coli / Q000737:chemistry*; D010457:Peptidoglycan / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D003432:Cross-Linking Reagents; D010457:Peptidoglycan