title: The influence of the density of adrenergic innervation on the extracellular steady-state concentration gradient for 3H-noradrenaline.
abstract: After inhibition of extraneuronal uptake by corticosterone, isolated right atria and lengthwise halved vasa deferentia of the rat were incubated with 0.2 mumol/l 3H-noradrenaline for 60 min, washed out for 100 min and then prepared for autoradiography. The autoradiographic images were digitized, and silver grain density was determined as a function of the distance from the surface. Silver grain density declined towards the centre of the tissue; the decline was monophasic exponential and significantly steeper in the vas deferens (0.016 microns-1) than in the less densely innervated right atrium (0.011 microns-1). Silver grain density at the surface of the tissue was higher in vas deferens than in right atrium. The results show that the extracellular steady-state concentration gradient for 3H-noradrenaline (generated by uptake1 during the incubation with this amine) largely depends on the density of the adrenergic innervation.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Universität Würzburg, Federal Republic of Germany.
authors.forename: E
authors.initials: E
authors.lastname: Schömig
authors.forename: I
authors.initials: I
authors.lastname: Azevedo
authors.forename: D
authors.initials: D
authors.lastname: Moura
authors.forename: U
authors.initials: U
authors.lastname: Trendelenburg
mesh_terms: D000818:Animals; D006325:Heart Atria / Q000294:innervation; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000032:analysis*; D051381:Rats; D013564:Sympathetic Nervous System / Q000502:physiology*; D014316:Tritium; D014649:Vas Deferens / Q000294:innervation
chemical_list: D014316:Tritium; D009638:Norepinephrine