Human immunodeficiency virus type 1 and hepatitis B virus transcription in peripheral blood lymphocytes from co-infected subjects.
The molecular characteristics of peripheral blood lymphocyte (PBL) infection by hepatitis B virus (HBV) were studied in human immunodeficiency virus type 1 (HIV-1) infected subjects using highly sensitive polymerase chain reaction (PCR) based techniques. DNA and RNA samples were purified from PBLs of HIV-1 infected individuals, regardless of their HBV serological status and assayed using PCR and reverse-transcription (RT) PCR, respectively. The data shown here are an extension of previous reports documenting HBV and HIV-1 co-infection of PBLs and indicate that transcriptionally active HBV infection of PBLs is detectable in a significant proportion of asymptomatic HIV-1 infected patients.
Clementi
M
M
Institute of Microbiology, University of Ancona, Italy.
Manzin
A
A
Bagnarelli
P
P
Menzo
S
S
Varaldo
P E
PE
Carloni
G
G
D004279:DNA, Viral / Q000235:genetics; D015658:HIV Infections / Q000097:blood / Q000150:complications / Q000382:microbiology*; D015497:HIV-1 / Q000235:genetics*; D006509:Hepatitis B / Q000097:blood / Q000150:complications / Q000382:microbiology*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
D004279:DNA, Viral; D012367:RNA, Viral