Beta-N-acetylhexosaminidase in the urine, kidney and serum of bromobenzene-intoxicated mice.
beta-N-Acetylhexosaminidase isoenzymes were separated from the kidney, serum and urine of normal mice and mice intoxicated with bromobenzene, using DEAE-cellulose chromatography. Both mouse serum and urine showed hexosaminidase profiles similar to the human counterparts with the presence of B (basic), I (intermediate) and A (acidic) isoenzymes. A notable feature was the presence of a high proportion of an intermediate form in mouse urine which is not always present in human urine. Hexosaminidase activity increased significantly in urine of mice intoxicated with bromobenzene. Its increase was time-dependent and due to kidney damage with a release in the urine of hexosaminidase A, I and, in higher proportion, B. No significant differences were observed in mouse kidney and serum profiles following intoxication with bromobenzene. The total activity of hexosaminidase, using 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside as substrate, did not increase in the serum of mice intoxicated with bromobenzene. Both hexosaminidase activity and the isoenzyme pattern in urine can be used as indicators of kidney damage by bromobenzene intoxication.
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Dipartimento di Medicina Sperimentale e Scienze Biochimiche, University of Perugia, Italy.
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D000818:Animals; D001969:Bromobenzenes / Q000506:poisoning*; D002848:Chromatography, DEAE-Cellulose; D054818:Hexosaminidase A; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008991:Monitoring, Physiologic / Q000379:methods; D001619:beta-N-Acetylhexosaminidases / Q000097:blood / Q000378:metabolism* / Q000652:urine
D001969:Bromobenzenes; C032036:bromobenzene; D054818:Hexosaminidase A; D001619:beta-N-Acetylhexosaminidases