title: Mammalian expression of the human sex steroid-binding protein of plasma (SBP or SHBG) and testis (ABP). Characterization of the recombinant protein.
abstract: A full-length 1,209 bp cDNA encoding the human sex steroid-binding protein of plasma (SBP or SHBG) and testis (ABP) was constructed and expressed in BHK-21 cells. The sequence agrees with the published gene and protein sequences. The cells were found to secrete SBP following transfection and G418r selection. The recombinant protein binds 5 alpha-dihydrotestosterone with a Kd of 0.28 nM. It also binds testosterone and 17 beta-estradiol but not progesterone, estrone or cortisol revealing a steroid-binding specificity identical to that of human SBP. SDS-PAGE patterns are less complex than human SBP and show a monomeric molecular weight of about 43 kDa.
authors.affiliation: Zymogenetics Inc., Seattle, WA 98105.
authors.forename: F S
authors.initials: FS
authors.lastname: Hagen
authors.forename: C
authors.initials: C
authors.lastname: Arguelles
authors.forename: L M
authors.initials: LM
authors.lastname: Sui
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: P R
authors.initials: PR
authors.lastname: Seidel
authors.forename: S C
authors.initials: SC
authors.lastname: Conroy
authors.forename: P H
authors.initials: PH
authors.lastname: Petra
mesh_terms: D001483:Base Sequence; D001769:Blood; D003001:Cloning, Molecular; D004247:DNA; D015870:Gene Expression; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009928:Organ Specificity / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012738:Sex Hormone-Binding Globulin / Q000235:genetics* / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D012738:Sex Hormone-Binding Globulin; D004247:DNA