Construction of a tetracycline-inducible promoter in Schizosaccharomyces pombe.
We have developed a tightly repressed Schizosaccharomyces pombe promoter which can be efficiently induced by Tetracycline. This promoter is a derivative of the plant viral cauliflower mosaic virus 35S promoter which normally functions as a strong constitutive promoter in S. pombe. Location of three binding sites for the Tn10-encoded Tet repressor in the vicinity of the TATA-box of the CaMV 35S promoter led to a tight repression of promoter activity in the presence of the Tet repressor protein. Up to a 400-fold induction was observed after addition of the inducer Tetracycline, which inactivates the operator-binding capacity of the repressor.
Faryar
K
K
Institut für Genbiologische Forschung Berlin GmbH, Federal Republic of Germany.
Gatz
C
C
D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*; D013752:Tetracycline / Q000494:pharmacology*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics
D005656:Fungal Proteins; D012097:Repressor Proteins; D015500:Chloramphenicol O-Acetyltransferase; D013752:Tetracycline