Immunohistochemical detection of c-myc products in colorectal cancer and proliferative cell rate.
Expression of c-myc protein was studied immunohistochemically in colorectal cancers using a monoclonal antibody, MYC-1. Immunoblotting assays with cellular lysates demonstrated a band of the gene products at the level of 60 kDa. c-myc-protein-positive tumor cells were observed in 43 (43.4%) of 99 specimens of colorectal cancers. There was no significant correlation between the incidence of MYC-1-positive tumors and clinicopathological findings. The rate of MYC-1 proteins occurring in patients with liver metastasis was significantly higher than that in patients without the metastasis. The rate of occurrence of DNA polymerase-alpha-positive cells in MYC-1 protein-positive tumors was significantly higher than in MYC-1 negative ones. The results suggested that MYC-1 immunoreactivity might possibly be a useful prognostic marker of colorectal cancers.
Yamaguchi
A
A
Department of Surgery II, School of Medicine, Kanazawa University, Ishikawa, Japan.
Ninomiya
I
I
Ishida
T
T
Nishimura
G
G
Kanno
M
M
Yonemura
Y
Y
Miwa
K
K
Miyazaki
I
I
Matsukawa
S
S
D000230:Adenocarcinoma / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D015153:Blotting, Western; D002455:Cell Division / Q000235:genetics; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D004257:DNA Polymerase II / Q000032:analysis; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis*
D016271:Proto-Oncogene Proteins c-myc; D004257:DNA Polymerase II