Pre-boiling high GC content, mixed primers with 3' complementation allows the successful PCR amplification of Pseudomonas aeruginosa DNA.
Kureishi
A
A
Department of Microbiology & Infectious Diseases, University of Calgary, Alberta, Canada.
Bryan
L E
LE
D001482:Base Composition / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D013696:Temperature
D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides