[The relationship between ploidy and morphological classification of human megakaryocytes].
The relation between polyploidy and morphological classification of human megakaryocytes was studied in bone marrow aspirates from five normal individuals. On a Wright-Giemsa stained smear, megakaryocytes were morphologically classified into four groups according to a modification of Feinendegen's classification which is considered to reflect megakaryocyte maturation. The DNA of the morphologically classified cell is measured by microcytofluorometry using DAPI (4',6-diamidino-2-phenylindole) staining after removing the Wright-Giemsa stain. Most of the normal megakaryocytes were classified into type III (mature megakaryocytes) and the maximum peak in population of the megakaryocyte ploidy was observed at 16N. In each individual, the ploidy showed a similar pattern regardless of the classification. These findings suggest that the development of ploidy depends on a factor different from the one that determined the megakaryocyte maturation of cytoplasm and the ploidy is determined at the level of a megakaryocyte precursor or the most juvenile megakaryocyte.
Kobayashi
Y
Y
1st Department of Internal Medicine, Kyoto Prefectural University of Medicine.
Maruo
N
N
Tanaka
K
K
Kimura
S
S
Wada
K
K
Ozawa
M
M
Horiuchi
H
H
Kondo
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D004247:DNA / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000737:chemistry / Q000166:cytology*; D011003:Ploidies; D011123:Polyploidy
D004247:DNA