title: Prognostic factors in bladder carcinoma: histologic parameters and expression of a cell cycle-related nuclear antigen (Ki-67).
abstract: A total of 316 biopsies from patients with bladder carcinoma who entered a trial of the genito-urinary group of the European Organization for Research and Treatment of Cancer (EORTC) were reviewed. The histological data were subsequently correlated with the clinical course. A strong correlation between the number of mitoses and the time of first recurrence, muscle invasion, and death was noted. Next the expression of Ki-67 in frozen sections from 49 transitional cell carcinomas (TCCs) and the DNA content of the tumour cells were determined. The frequency of Ki-67-positive tumour cells increased with tumour grade and stage. Grade II TCCs and superficially infiltrating TCCs showed a wide range of Ki-67 scores. There was a significant difference in Ki-67 score between non-infiltrating (Ta) and superficially infiltrating (T1) grade II TCCs. All DNA-aneuploid carcinomas but also 15 out of 36 DNA-diploid tumours contained more than 10 per cent Ki-67-positive cells. Only some of these tumours were DNA-aneuploid or -tetraploid. The results indicate that the number of Ki-67-positive cells in grade II tumours may be a useful aid in separating grade II TCCs with a favourable prognosis from those with a poor clinical outcome.
authors.affiliation: Department of Pathology, Academic Hospital Dijkzigt, Erasmus University, Rotterdam, The Netherlands.
authors.forename: A H
authors.initials: AH
authors.lastname: Mulder
authors.forename: J C
authors.initials: JC
authors.lastname: Van Hootegem
authors.forename: R
authors.initials: R
authors.lastname: Sylvester
authors.forename: F J
authors.initials: FJ
authors.lastname: ten Kate
authors.forename: K H
authors.initials: KH
authors.lastname: Kurth
authors.forename: E C
authors.initials: EC
authors.lastname: Ooms
authors.forename: T H
authors.initials: TH
authors.lastname: Van der Kwast
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D034961:Antigens, Nuclear; D002295:Carcinoma, Transitional Cell / Q000276:immunology / Q000473:pathology*; D002455:Cell Division / Q000502:physiology; D006801:Humans; D007124:Immunoenzyme Techniques; D008938:Mitosis / Q000502:physiology; D009687:Nuclear Proteins / Q000032:analysis*; D011379:Prognosis; D001749:Urinary Bladder Neoplasms / Q000276:immunology / Q000473:pathology*
chemical_list: D000951:Antigens, Neoplasm; D034961:Antigens, Nuclear; D009687:Nuclear Proteins