Detection of Campylobacter upsaliensis in diarrheic dogs and cats, using a selective medium with cefoperazone.
Using a newly formulated selective medium containing cefoperazone, we isolated 72 Campylobacter strains in fecal samples from 397 diarrheic dogs and cats. Of these, 39 were thermophilic catalase-negative Campylobacter species. We identified these Campylobacter strains by DNA:DNA hybridization, using digoxigenin-labeled total genomic DNA of 4 Campylobacter reference strains (C jejuni, C coli, C lari, and C upsaliensis) as a probe. The labeling was done with a commercially available kit. We could identify 66 of the 72 Campylobacter isolates to the species level with this method; identification with probes always agreed with conventional test results. Of the 66 identified strains, 33 were C upsaliensis and 33 were C jejuni. Six isolates could not be assigned to a known species with probes or conventional tests. On the basis of our findings, C upsaliensis is more resistant to cefoperazone than to cephalothin, thereby explaining the unexpected recovery of these campylobacters on cephalosporin-containing media.
Burnens
A P
AP
Institute of Veterinary Bacteriology, University of Berne, Switzerland.
Nicolet
J
J
D000818:Animals; D002167:Campylobacter / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D002438:Cefoperazone / Q000494:pharmacology; D002512:Cephalothin / Q000494:pharmacology; D003470:Culture Media; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D004352:Drug Resistance, Microbial; D005243:Feces / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D011933:Reagent Kits, Diagnostic / Q000662:veterinary
D003470:Culture Media; D015342:DNA Probes; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; D002438:Cefoperazone; D002512:Cephalothin