Effect of local hemostatics on bone induction in rats: a comparative study of bone wax, fibrin-collagen paste, and bioerodible polyorthoester with and without gentamicin.
Local hemostatics for osseous tissue should preferably be absorbable and biocompatible and should not inhibit osteogenesis. The tissue response and effect on demineralized bone-induced heterotopic osteogenesis in the abdominal muscle of 120 male Wistar rats by different local hemostatics were evaluated by light microscopy and 85Sr uptake analyses. Non-absorbable bone wax of 88% beeswax and absorbable bovine fibrin-collagen paste both significantly inhibited osteoinduction, whereas a bioerodible polyorthoester drug delivery system with or without 4% gentamicin did not. Bone wax was not absorbed and induced a chronic foreign body reaction. Fibrin-collagen paste induced less inflammation with numerous monocytes and macrophages with engulfed material. Bioerodible polyorthoester caused a very moderate tissue reaction and was mostly resorbed at week 4.
Solheim
E
E
Institute for Surgical Research, Rikshospitalet, University of Oslo, Norway.
Pinholt
E M
EM
Bang
G
G
Sudmann
E
E
D000042:Absorption; D000818:Animals; D001672:Biocompatible Materials / Q000493:pharmacokinetics / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects* / Q000473:pathology; D003094:Collagen / Q000493:pharmacokinetics / Q000494:pharmacology; D004338:Drug Combinations; D005337:Fibrin / Q000493:pharmacokinetics / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D006490:Hemostatics / Q000493:pharmacokinetics / Q000494:pharmacology*; D007249:Inflammation / Q000209:etiology / Q000473:pathology; D008297:Male; D008422:Materials Testing; D010012:Osteogenesis / Q000187:drug effects; D010168:Palmitates / Q000493:pharmacokinetics / Q000494:pharmacology; D011091:Polyesters / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014885:Waxes / Q000493:pharmacokinetics / Q000494:pharmacology
D001672:Biocompatible Materials; D004338:Drug Combinations; D005839:Gentamicins; D006490:Hemostatics; D010168:Palmitates; D011091:Polyesters; D014885:Waxes; C055480:bone wax; C045986:Alzamer; D005337:Fibrin; D003094:Collagen