Gene probes for the detection of 6-deoxyhexose metabolism in secondary metabolite-producing streptomycetes.
DNA probes were designed from the streptomycin production genes strDELM of Streptomyces griseus involved in the biosynthesis of the 6-deoxyhexose (6DOH) dihydrostreptose which could detect the genomic fragments coding for 6DOH formation in other actinomycetes strains. In about 70% of the 43 strains tested at least one signal could be detected with strD-, strE- or strLM-specific probes. Evidence is presented that the hybridizing genes are mostly clustered and probably engaged in the formation of secondary metabolites. Because of the wide-spread use of 6DOH constituents in natural products these probes should allow to detect a vast array of different secondary metabolic gene clusters in actinomycetes.
Stockmann
M
M
Bergische Universität GH, Wuppertal, F.R.G.
Piepersberg
W
W
D015342:DNA Probes; D003837:Deoxy Sugars / Q000096:biosynthesis / Q000378:metabolism*; D005798:Genes, Bacterial; D006601:Hexoses / Q000096:biosynthesis / Q000378:metabolism*; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D013305:Streptomyces griseus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
D015342:DNA Probes; D003837:Deoxy Sugars; D006601:Hexoses; C007418:dihydrostreptose