title: Molecular genetic analysis of the Escherichia coli phoP locus.
abstract: We have cloned the Escherichia coli phoP gene, a member of the family of environmentally responsive two-component systems, and found its deduced amino acid sequence to be 93% identical to that of the Salmonella typhimurium homolog, which encodes a major virulence regulator necessary for intramacrophage survival and resistance to cationic peptides of phagocytic cells. The phoP gene was mapped to kilobase 1202 on the Kohara map (25-min region) of the E. coli genome (Y. Kohara, K. Akiyama, and K. Isono, Cell 50:495-508, 1987) and found to be transcribed in a counterclockwise direction. Both E. coli and S. typhimurium phoP mutants were more sensitive than their isogenic wild-type strains to the frog-derived antibacterial peptide magainin 2, suggesting a role for PhoP in the response to various stresses in both enteric species.
authors.affiliation: Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110-1093.
authors.forename: E A
authors.initials: EA
authors.lastname: Groisman
authors.forename: F
authors.initials: F
authors.lastname: Heffron
authors.forename: F
authors.initials: F
authors.lastname: Solomon
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D010583:Bacteriophage mu / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012093:Replicon; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D014157:Transcription Factors; C061064:PhoP protein, Bacteria