title: Molecular cloning and characterization of catechol 2,3-dioxygenases from biphenyl/polychlorinated biphenyls-degrading bacteria.
abstract: Catechol 2,3-dioxygenases were cloned from Alcaligenes sp. KF711, Pseudomonas putida KF715, and Achromobacter xylosoxidans KF701 which are biphenyl/polychlorinated biphenyls-degrading bacteria. All of the cloned enzymes were purified by preparative polyacrylamide gel electrophoresis (PAGE). The purified catechol 2,3-dioxygenases were significantly different from one another in ring-fission activities to catechol and its derivatives. The catechol 2,3-dioxygenase from Alcaligenes sp. KF711 exhibited higher ring-fission activity to 4-chlorocatechol than those from P. putida KF715 and A. xylosoxidans KF701. In electrophoretic mobilities, the three enzymes were different from one another on nondenaturing PAGE but the same on SDS-PAGE.
authors.affiliation: College of Pharmacy, Chungbuk National University, Korea.
authors.forename: H
authors.initials: H
authors.lastname: Chang
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Roh
authors.forename: S R
authors.initials: SR
authors.lastname: Kim
authors.forename: K R
authors.initials: KR
authors.lastname: Min
authors.forename: C K
authors.initials: CK
authors.lastname: Kim
authors.forename: E G
authors.initials: EG
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000421:Alcaligenes / Q000201:enzymology* / Q000235:genetics; D001713:Biphenyl Compounds / Q000378:metabolism*; D050557:Catechol 2,3-Dioxygenase; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D049308:Dioxygenases; D004591:Electrophoresis, Polyacrylamide Gel; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D001713:Biphenyl Compounds; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D011078:Polychlorinated Biphenyls; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase