Retinoid augmentation of bioactive interleukin-1 production by murine keratinocytes.
The effect of retinoids on the production of interleukin-1 (IL-1) by murine epidermal keratinocytes was investigated. Freshly isolated keratinocytes were cultured in the presence of etretinate, acitretin, all-trans retinoic acid or 13-cis retinoic acid at concentrations of 8 x 10(-9)-8 x 10(-6) mol/l. Exposure of keratinocytes to retinoids increased IL-1 bioactivity in culture supernatants and cell extracts at concentrations as low as 8 x 10(-9) mol/l, as assessed by T-cell proliferation. Prolongation of the culture period enhanced the augmentative effect of retinoids. All-trans retinoic acid and 13-cis retinoic acid had a greater ability to induce IL-1 production than the two aromatic retinoids, etretinate and acitretin. Treatment with 8-methoxypsoralen plus ultraviolet A radiation and treatment with triamcinolone acetonide both reduced the effect of retinoids on the production of bioactive IL-1.
Tokura
Y
Y
Department of Dermatology, Yale University School of Medicine, New Haven.
Edelson
R L
RL
Gasparro
F P
FP
D017255:Acitretin; D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005050:Etretinate / Q000494:pharmacology; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis*; D015474:Isotretinoin / Q000494:pharmacology; D015603:Keratinocytes / Q000378:metabolism*; D008730:Methoxsalen / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D012176:Retinoids / Q000494:pharmacology*; D014212:Tretinoin / Q000031:analogs & derivatives / Q000494:pharmacology; D014222:Triamcinolone Acetonide / Q000494:pharmacology; D014466:Ultraviolet Rays
D007375:Interleukin-1; D012176:Retinoids; D014212:Tretinoin; D005050:Etretinate; D004247:DNA; D015474:Isotretinoin; D014222:Triamcinolone Acetonide; D017255:Acitretin; D008730:Methoxsalen