Antifibrotic and uveitogenic properties of gamma interferon in the rabbit eye.
The feasibility of gamma (gamma)-interferon injection for the treatment of ocular fibrotic conditions was studied in rabbits using recombinant rabbit gamma-interferon and the cell-injection model of tractional detachment. A toxicity study revealed that intravitreal injection of greater than 10(4) units gamma-interferon consistently produced panuveitis. For tractional detachment, 250,000 rabbit dermal fibroblasts were injected intravitreally into 20 eyes; 1 day later, 5 of these eyes received intravitreal injections of 10(4) units gamma-interferon, another 5 were given 10(6) units, and the remaining 10 received balanced salt solution. Slit-lamp examination and fundus photography were performed at regular intervals for 21 days and were graded by a masked observer. The eyes were then enucleated and processed for histology. Doses of 10(4) units gamma-interferon significantly reduced the severity of detachments, but injections of 10(6) units induced panuveitis.
Hjelmeland
L M
LM
Department of Ophthalmology, School of Medicine, University of California, Davis 95616.
Li
J W
JW
Toth
C A
CA
Landers
M B
MB
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005128:Eye Diseases / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects; D005355:Fibrosis; D007371:Interferon-gamma / Q000633:toxicity*; D015864:Panuveitis / Q000139:chemically induced* / Q000473:pathology; D011817:Rabbits; D011994:Recombinant Proteins; D012163:Retinal Detachment / Q000473:pathology / Q000517:prevention & control*; D012164:Retinal Diseases / Q000473:pathology; D014822:Vitreous Body / Q000473:pathology
D011994:Recombinant Proteins; D007371:Interferon-gamma