Leishmania donovani: purification and partial characterization of a glycophosphosphingolipid antigen expressed on promastigote surface.
A ceramide-anchored glycophosphosphingolipid antigen was isolated from the lipid extract of Leishmania donovani promastigotes. The affinity-purified glycolipid antigen contained galactose, mannose, myo-inositol, phosphate, ceramide, and hexosamine but no sialic acid. The phosphate group was present internally at the core of the structure: inositol (1-O)-phosphorylceramide. The phosphate group became susceptible to alkaline phosphatase only after alkali-catalyzed hydrolysis of the glycolipid.
De-Majumdar
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Indian Institute of Chemical Biology, Calcutta.
D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D006028:Glycosphingolipids / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D017261:Glycosylphosphatidylinositols; D007893:Leishmania donovani / Q000276:immunology*; D010716:Phosphatidylinositols / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D010743:Phospholipids / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*
D000953:Antigens, Protozoan; D002241:Carbohydrates; D005227:Fatty Acids; D006017:Glycolipids; D006028:Glycosphingolipids; D017261:Glycosylphosphatidylinositols; D010716:Phosphatidylinositols; D010743:Phospholipids