[Immunohistochemical analysis of the distribution of H4 antigen in normal and cancer cells of the skin and uterine cervix].
The aim of the work was immunohistochemical localization of antigen H-4 in the epithelium of skin, uterine cervix and their malignant tumours. Mouse monoclonal antibodies (MABs) against antigen H-4 obtained by the hybridoma method after immunization of mice with rat colon epithelium cytoskeletal fractions were used. Cryostat sections of various types of epithelium and human tumours showed MABs H-4 to react with the cells of simple single-layered, metaplastic squamous epithelium, non-cornified and cornified squamous epithelia as well as with the cells of malignant tumours from these epithelia. Myoepithelial cells of eccrine sweat glands and basal cells of ectocervix were areactive. The localization of antigen H-4 in the intermediate filaments of epithelial cells and cells of squamous cell carcinoma of the skin and uterine cervix was revealed by means of ultrastructural immunocytochemistry. MABs H-4 seem to mark either a general epitope of a number of cytokeratin peptides or cytokeratin-associated protein. MABs H-4 are suggested to be used as a universal immunohistochemical marker of epithelium.
Petrov
S V
SV
Raĭkhlin
N T
NT
D000328:Adult; D000941:Antigens / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002584:Cervix Uteri / Q000166:cytology / Q000276:immunology* / Q000473:pathology; D004848:Epithelium / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D012016:Reference Values; D012867:Skin / Q000166:cytology / Q000276:immunology* / Q000473:pathology; D012878:Skin Neoplasms / Q000276:immunology* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000276:immunology* / Q000473:pathology
D000941:Antigens; D000951:Antigens, Neoplasm
Immunogistokhimicheskiĭ analiz raspredeleniia antigena H4 v normal'nykh i rakovykh kletkakh kozhi i sheĭki matki.