Chloroplast and cytoplasmic enzymes: isolation and sequencing of cDNAs coding for two distinct pea chloroplast aldolases.
Two cDNAs which correspond to two very similar Class I aldolases have been isolated from a pea (Pisum sativum L.) cDNA library. With the exception of one codon they match the experimentally determined N-terminal sequence of a pea chloroplast aldolase. The deduced C-terminal sequence of one of these clones is unique among Class I aldolases. The deduced C-terminus of the other is more like the C-terminus of other eucaryotic Class I aldolases. Comparisons of sequence homology suggest that the pea chloroplast isozymes are only marginally more closely related to the anaerobically induced plant aldolases than to aldolases from animals.
Razdan
K
K
Department of Biological Sciences, University of Illinois, Chicago 60680.
Heinrikson
R L
RL
Zurcher-Neely
H
H
Morris
P W
PW
Anderson
L E
LE
D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000201:enzymology*; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000201:enzymology; D004247:DNA / Q000235:genetics; D007887:Fabaceae; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D015183:Restriction Mapping; D016415:Sequence Alignment; D013045:Species Specificity
D004247:DNA; D005634:Fructose-Bisphosphate Aldolase