title: The nucleotide sequence of the coat protein gene and 3' untranslated region of papaya ringspot virus type W (Aust).
abstract: The nucleotide sequence of the 3' terminal region of the Australian isolate of papaya ringspot virus type W [PRSV-W (Aust)] was determined. An open reading frame (864 bp), encoding the putative coat protein gene, occurs upstream of the putative 3' untranslated region (206 bp) and poly(A) tail. A 23 amino acid sequence was obtained from N-terminal analysis of the coat protein from purified virions. This sequence has 100% homology with a region of the amino acid sequence inferred from the nucleic acid sequence of the coat protein gene. However, this region is 13 amino acids downstream from the N terminus predicted for two American isolates of PRSV. The coat protein gene of PRSV-W (Aust) was shown to have 96.8% and 96.4% nucleotide sequence similarity with American isolates of PRSV-W and PRSV-P, respectively.
authors.affiliation: Centre for Molecular Biotechnology, Queensland University of Technology, Brisbane, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Bateson
authors.forename: J
authors.initials: J
authors.lastname: Dale
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D015342:DNA Probes; D004279:DNA, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D036022:Capsid Proteins; D015342:DNA Probes; D004279:DNA, Viral; C073700:coat protein, Papaya ringspot virus