A new gene of the f1 operon of Y. pestis involved in the capsule biogenesis.
The DNA sequence determination of the f1 operon between the genes encoding the F1 subunit (caf1) and chaperone-like protein (caf1M) revealed a large open reading frame that codes for a polypeptide similar to some E. coli proteins involved in the biogenesis of fimbria. The deletion and in trans complementation analyses showed that this gene is not necessary for extracellular transport of the F1 subunit but plays a role in the capsule assembly.
Karlyshev
A V
AV
Institute of Immunology, State Concern Biopreparation, Lyubuchany, Moscow Region, USSR.
Galyov
E E
EE
Smirnov OYu
Guzayev
A P
AP
Abramov
V M
VM
Zav'yalov
V P
VP
D000595:Amino Acid Sequence; D016667:Bacterial Capsules / Q000096:biosynthesis*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D015183:Restriction Mapping; D016415:Sequence Alignment; D015010:Yersinia pestis / Q000235:genetics*
D004269:DNA, Bacterial