title: Sensitization primes platelet-activating factor (PAF)-induced accumulation of eosinophils in mouse skin lesions: contribution of cytokines to the response.
abstract: We have examined the hypothesis that cytokines mediate the enhanced responsiveness of eosinophils to PAF in sensitized mouse skin. PAF (10 ng per site) resulted in a considerable degree of eosinophil accumulation in ovalbumin (OA)-sensitized mice but not in non-sensitized mice. Intradermal preadministration of cytokines (IL-5, IL-3 and GM-CSF) also significantly enhanced PAF-induced migration of eosinophils in a dose-dependent manner. The relative potency with which these cytokines primed cell migration was IL-5 greater than IL-3 greater than GM-CSF, however, each cytokine alone showed no direct effect. We conclude that the sensitization or the exogenous application of cytokines is capable of augmenting PAF-induced eosinophil migration in mice in vivo, and the cytokines thus elicited by sensitization may contribute to the extensive recruitment of inflammatory cells in allergic diseases.
authors.affiliation: Department of Medicine and Clinical Immunology, Dokkyo University School of Medicine, Tochigi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yukawa
authors.forename: K
authors.initials: K
authors.lastname: Terashi
authors.forename: Y
authors.initials: Y
authors.lastname: Terashi
authors.forename: M
authors.initials: M
authors.lastname: Arima
authors.forename: H
authors.initials: H
authors.lastname: Sagara
authors.forename: S
authors.initials: S
authors.lastname: Motojima
authors.forename: T
authors.initials: T
authors.lastname: Fukuda
authors.forename: S
authors.initials: S
authors.lastname: Makino
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology*; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D015848:Interleukin-5 / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010972:Platelet Activating Factor / Q000494:pharmacology*; D012867:Skin / Q000166:cytology*
chemical_list: D016207:Cytokines; D007377:Interleukin-3; D015848:Interleukin-5; D010972:Platelet Activating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor