title: Microbial contamination of enteral feeding solution and its prevention.
abstract: In an investigation of microbial contamination of enteral feeding solutions, all 22 residual solutions obtained immediately after administration were contaminated at concentrations of 10(3) to 10(6) viable counts/ml. Major contaminants were glucose-nonfermenting gram-negative bacilli such as Pseudomonas aeruginosa and Acinetobacter calcoaceticus var anitratus. Contamination seemed to have been caused by frequent reuse of bag-type containers and the infusion tubes connected to the bags, neither of which can be washed or dried. Decontamination methods were evaluated by using polypropylene containers that can be washed and disinfected for administration. Few Serratia marcescens on the inside wall of the container were removed by rinsing with tap water, alone or in combination with detergent scrub. Tap water and detergent plus air-drying at 56 degrees C for 1 hour reduced Serratia marcescens only somewhat. Tap water and detergent plus immersion in 0.01% sodium hypochlorite for 1 hour or in water at 70 degrees C for 3 minutes eliminated all 10(11) cells of Serratia marcescens.
authors.affiliation: Department of Pharmacy, Yamaguchi University Hospital, Ube, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Oie
authors.forename: A
authors.initials: A
authors.lastname: Kamiya
authors.forename: K
authors.initials: K
authors.lastname: Hironaga
authors.forename: A
authors.initials: A
authors.lastname: Koshiro
mesh_terms: D015169:Colony Count, Microbial; D004203:Disinfection / Q000379:methods / Q000592:standards; D004209:Disposable Equipment / Q000592:standards; D004750:Enteral Nutrition / Q000295:instrumentation / Q000592:standards*; D004867:Equipment Design / Q000592:standards; D005069:Evaluation Studies as Topic; D005516:Food Microbiology / Q000592:standards*; D005526:Food, Formulated / Q000382:microbiology* / Q000592:standards; D006801:Humans; D017053:Infection Control / Q000379:methods