Growth-related production of proteoglycans and hyaluronic acid in synchronous arterial smooth muscle cells.
1. The growth-stimulating effect of serum on the proteoglycan and hyaluronic acid production in arterial smooth muscle cells was investigated, using cells synchronized by serum deprivation. 2. After stimulation, synthesis of [35S]sulfated proteoglycans and [14C]hyaluronic acid increased during G1 and G2 phases (about 2- and 5-fold, respectively, in the culture medium), in comparison with quiescent cells. 3. Neither the size, nor the charge, nor the relative proportions of [35S]glycosaminoglycans of the proteoglycans were modified. 4. However, when the cells were stimulated to divide, increased synthesis of large [14C]hyaluronic acid was observed concomitantly with the production of higher hydrodynamic size [35S]proteoglycans, which aggregated with hyaluronic acid (20%).
Deudon
E
E
Laboratoire de Biochimie, INSERM-U.181, Faculté de médecine Saint-Antoine, Paris, France.
Berrou
E
E
Breton
M
M
Picard
J
J
D000818:Animals; D001011:Aorta; D001769:Blood; D002455:Cell Division; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D003871:Dermatan Sulfate / Q000096:biosynthesis; D016193:G1 Phase; D016195:G2 Phase; D006025:Glycosaminoglycans / Q000378:metabolism; D006497:Heparan Sulfate / Q000096:biosynthesis; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis*; D013462:Sulfur Radioisotopes; D013552:Swine
D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D011509:Proteoglycans; D013462:Sulfur Radioisotopes