title: Structure of the gene complementing uvr-402 in Streptococcus pneumoniae: homology with Escherichia coli uvrB and the homologous gene in Micrococcus luteus.
abstract: The repair ability for UV-induced damage observed for Streptococcus pneumoniae proceeds through a system similar to the Uvr-dependent system in Escherichia coli. The DNA sequence of a gene complementing uvr-402, a mutation conferring UV sensitivity, was determined. Alignments of the deduced amino acid sequence revealed an extensive sequence homology of 55% with the UvrB protein of E. coli and 59% with the UvrB-homologous protein of Micrococcus luteus. Nucleotide-binding site consensus was observed. The high conservation of the uvrB-like gene among these three species suggests that the role of the UvrB protein and excision repair in general might be very important for cell survival.
authors.affiliation: Centre National de la Recherche Scientifique, Université Paul Sabatier, Toulouse, France.
authors.forename: N
authors.initials: N
authors.lastname: Sicard
authors.forename: J
authors.initials: J
authors.lastname: Oreglia
authors.forename: A M
authors.initials: AM
authors.lastname: Estevenon
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016982:Micrococcus luteus / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013296:Streptococcus pneumoniae / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays