title: Systemic-oral immunization with 56 kDa molecule of Giardia lamblia affords protection in experimental mice.
abstract: Prior systemic-oral immunization of inbred mice with Giardia lamblia surface-associated antigen of molecular mass 56 kDa not only significantly blocked colonization but also resulted in elimination of G. lamblia trophozoites by 9-11 days following challenge. The colonization and multiplication of the trophozoites in unprotected animals were accompanied by a pronounced influx of suppressor T cells in intraepithelial or lamina propria of the small intestine and a significant decline in IgA-bearing plasma cells in the lamina propria. An induction of helper/inducer T cells in the intraepithelial and lamina propria and significant enhancement of IgA and IgG-bearing cells in the lamina propria of the small gut resulted in a decline, and eventual elimination, of the trophozoites from the gut. The completion of the immunization of animals with 56 kDa G. lamblia antigen resulted in: significant enhancement of helper/inducer T lymphocytes with no effect on suppressor T cells in the intraepithelial and lamina propria of the small gut; significant enhancement of IgA- and IgG-bearing plasma cells in lamina propria; and significant elevation of antibodies to 56 kDa G. lamblia antigen in the systemic circulation. The stimulation of such effector mechanisms in 56 kDa-immunized animals appears to result in failure of the trophozoites to get established, prevention of multiplication and earlier elimination from the gut. The data suggest that the 56 kDa molecule of G. lamblia immunoregulates the giardial infection.
authors.affiliation: Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Vinayak
authors.forename: K
authors.initials: K
authors.lastname: Kum
authors.forename: R
authors.initials: R
authors.lastname: Khanna
authors.forename: M
authors.initials: M
authors.lastname: Khuller
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology / Q000382:microbiology; D016829:Giardia lamblia / Q000276:immunology*; D005873:Giardiasis / Q000276:immunology / Q000517:prevention & control*; D007070:Immunoglobulin A / Q000276:immunology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000276:immunology; D007583:Jejunum / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D016131:Lymphocyte Subsets / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D014611:Vaccination
chemical_list: D000906:Antibodies; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D007070:Immunoglobulin A