title: Hyperresponsiveness to dietary cholesterol in inbred rabbits is not associated with enhanced reduction in binding of beta-VLDL to liver membranes.
abstract: In two inbred strains of rabbits with high or low response of serum cholesterol to dietary cholesterol, binding of rabbit beta-VLDL to hepatic membrane preparations was determined. The objective was to test the hypothesis that after cholesterol feeding, hyperresponders show a more dramatic reduction in hepatic apolipoprotein (apo) B/E receptors, which may explain the development of the high degree of cholesterolemia in these animals. The number of hepatic high affinity receptors for beta-VLDL in hyperresponders fed a diet without added cholesterol was, on average, 20% lower than that in hyporesponders. After the addition of increasing amounts of cholesterol to the diet, liver cholesterol concentrations were elevated to a greater extent in hyper- than in hyporesponsive rabbits. Liver free cholesterol concentrations were negatively associated with maximal binding of beta-VLDL to liver membranes. With increasing liver free cholesterol concentrations, maximal binding was less effectively depressed in hyper- than hyporesponders. We conclude that in the inbred strains of rabbits, hyperresponsiveness to dietary cholesterol is not caused by enhanced depression of hepatic apo B/E receptors.
authors.affiliation: Department of Laboratory Animal Science, University of Utrecht, The Netherlands.
authors.forename: G W
authors.initials: GW
authors.lastname: Meijer
authors.forename: A C
authors.initials: AC
authors.lastname: Beynen
authors.forename: M R
authors.initials: MR
authors.lastname: Lovati
authors.forename: C
authors.initials: C
authors.lastname: Manzoni
authors.forename: L F
authors.initials: LF
authors.lastname: Van Zutphen
authors.forename: C R
authors.initials: CR
authors.lastname: Sirtori
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011817:Rabbits
chemical_list: D002791:Cholesterol, Dietary; D008079:Lipoproteins, VLDL