Inhibition of replication of HIV-1 by retroviral vectors expressing tat-antisense and anti-tat ribozyme RNA.
A ribozyme was constructed that specifically cleaves RNA that contains the first coding exon of the tat gene of HIV-1. This anti-tat ribozyme was incorporated into a Moloney murine leukemia virus vector. A sequence containing only the 48-nucleotide antisense region of the ribozyme was also inserted into the retroviral vector. Human T-cell lines constitutively producing the tat-antisense and the anti-tat ribozyme RNA were created by transduction into Jurkat cells. When challenged with HIV-1, both the tat-antisense and anti-tat ribozyme-producing cells inhibited the replication of HIV-1. The antisense vector conferred a greater resistance to HIV-1 replication than did the anti-tat ribozyme vector.
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Division of Human Retrovirology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
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D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D004279:DNA, Viral; D015696:Gene Products, tat / Q000235:genetics*; D015726:Giant Cells / Q000166:cytology; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D016372:RNA, Antisense / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D013601:T-Lymphocytes / Q000382:microbiology; D014779:Virus Replication / Q000235:genetics*; D054322:tat Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015696:Gene Products, tat; D016372:RNA, Antisense; D016337:RNA, Catalytic; D012367:RNA, Viral; D054322:tat Gene Products, Human Immunodeficiency Virus