title: The nucleotide sequence of the infectious cloned DNA component of tobacco yellow dwarf virus reveals features of geminiviruses infecting monocotyledonous plants.
abstract: An infectious clone of the Australian geminivirus tobacco yellow dwarf virus (TobYDV) was constructed from virus-specific double-stranded DNA isolated from infected tobacco and used to demonstrate a single-component genome. The nucleotide sequence of TobYDV DNA comprises 2580 nucleotides. TobYDV DNA has three coding regions, two in the virion sense and one in the complementary sense, homologous to those identified for other geminiviruses, particularly those infecting monocotyledonous (monocot) plants. The complementary sense coding region is comprised of two overlapping reading frames, with an intron of 86 nucleotides. Efficient splicing of the mRNA for this coding region was observed in the infected dicotyledonous (dicot) hosts bean and tobacco despite the intron having an A + U content (57%) more typical of geminiviruses of monocot plants. TobYDV encapsidates a small oligonucleotide able to prime synthesis of the complementary DNA strand in vitro. The TobYDV genome organization, low A + U intron, and encapsidated oligonucleotide primer resemble those of the monocot-infecting geminiviruses. These results strongly suggest that TobYDV is a monocot geminivirus which has become adapted to dicot hosts.
authors.affiliation: Molecular Biology Group, DSIR Plant Protection, Auckland, New Zealand.
authors.forename: B A
authors.initials: BA
authors.lastname: Morris
authors.forename: K A
authors.initials: KA
authors.lastname: Richardson
authors.forename: A
authors.initials: A
authors.lastname: Haley
authors.forename: X
authors.initials: X
authors.lastname: Zhan
authors.forename: J E
authors.initials: JE
authors.lastname: Thomas
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D007438:Introns; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010942:Plant Viruses / Q000235:genetics*; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014026:Nicotiana / Q000382:microbiology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D014764:Viral Proteins; D015678:Viral Structural Proteins