Details of an isolation method for hepatic lymphocytes in mice.
The liver comprises a unique lymphocyte population, i.e., extrathymic alpha beta T cells with TcR of intermediate intensity. In the present study, we attempted to determine what pretreatments were appropriate to isolate hepatic mononuclear cells (MNC) containing such intermediate alpha beta TcR cells in mice. Hepatic MNC were isolated from untreated mice and mice subjected to either bleeding or liver perfusion, and the intermediate alpha beta TcR cells in each preparation were identified. For reasons of simplicity, cell purity and cell yields, hepatic lymphocytes should be obtained from mice subjected to total bleeding. Additional information on extrathymic alpha beta T cells obtained by using the recommended method is also presented.
Watanabe
H
H
Department of Immunology, Niigata University School of Medicine, Japan.
Ohtsuka
K
K
Kimura
M
M
Ikarashi
Y
Y
Ohmori
K
K
Kusumi
A
A
Ohteki
T
T
Seki
S
S
Abo
T
T
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001212:Asialoglycoproteins / Q000032:analysis; D017252:CD3 Complex; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002469:Cell Separation / Q000379:methods; D007694:Killer Cells, Natural / Q000276:immunology; D008099:Liver / Q000737:chemistry*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D013154:Spleen / Q000166:cytology
D000945:Antigens, Differentiation, T-Lymphocyte; D001212:Asialoglycoproteins; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D011947:Receptors, Antigen, B-Cell; D011948:Receptors, Antigen, T-Cell