title: Induction of premature centromere division affecting all chromosomes under culture conditions of fragile site expression.
abstract: In a study of chromosome fragility carried out under folate and thymidine deficiency conditions, we observed a seven- to ninefold increase of the incidence of premature centromere divisions (PCDs) affecting all chromosomes. This early separation of centromeres is clearly a culture effect and distinct from PCD and centromere splitting (CS), which imply a defect in the centromere of one or more chromosomes.
authors.affiliation: Departament de Biologia Cellular i Fisiologia, Facultat de Medicina, Universitat Autònoma de Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Fuster
authors.forename: R
authors.initials: R
authors.lastname: Miró
authors.forename: L
authors.initials: L
authors.lastname: Barrios
authors.forename: J
authors.initials: J
authors.lastname: Egozcue
mesh_terms: D002478:Cells, Cultured; D002503:Centromere / Q000187:drug effects / Q000648:ultrastructure*; D002871:Chromosome Banding; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D006801:Humans; D007621:Karyotyping; D008727:Methotrexate / Q000494:pharmacology*; D012016:Reference Values; D013936:Thymidine / Q000378:metabolism
chemical_list: D013936:Thymidine; D008727:Methotrexate