Effects of T cells on platelet-derived growth factor-like protein secretion from endothelial cells.
The cause of accelerated graft arteriosclerosis after heart transplantation is unknown. To examine whether the interactions of T cells and endothelial cells (ECs) could contribute to the cause of this phenomenon, T cells were co-incubated with human umbilical artery endothelial cells (HUAEC) and human vein endothelial cells (HUVEC) and the resulting conditioned medium supernatant was assayed for the presence of platelet-derived growth factor (PDGF)-like protein. PDGF-like protein secretion was significantly greater from HUAECs co-incubated with T cells at T-cell/HUAEC ratios of 30:1 (6.9 +/- 1.1 fmol/10(6) ECs) and 10:1 (6.0 +/- 1.1 fmol/10(6) ECs) than the combined background secretion from HUAECs and T cells incubated separately (3.6 +/- 0.8 fmol/10(6) ECs) (p less than 0.05). PDGF-like protein secretion above background levels from HUAECs was significantly greater, however, than from HUVECs co-incubated with T cells at T-cell/HUVEC ratios of 30:1 (1.0 +/- 0.4 fmol/10(6) ECs) and 10:1 (0.75 +/- 0.36 fmol/10(6) ECs) (p less than 0.05). In four experiments, preincubation of HUAECs with gamma-interferon induced HLA-DR antigen expression but actually caused a decrease in T-cell-induced PDGF-like protein secretion above background levels (3.0 +/- 0.6 fmol/10(6) ECs) when compared to nonstimulated HUAECs (4.0 +/- 0.4 fmol/10(6) ECs; p less than 0.05). PDGF-like protein secretion was minimal at 1 hour and increased over time to a maximum at 24 hours. The conclusion is that T cells are capable of inducing secretion of a very potent mitogen, PDGF-like protein, from endothelial cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Shaddy
R E
RE
Department of Pediatrics, University of Utah School of Medicine, Salt Lake City.
Hansen
J C
JC
Cowley
C G
CG
D016475:3T3 Cells; D000818:Animals; D003324:Coronary Artery Disease / Q000276:immunology; D003470:Culture Media; D004730:Endothelium, Vascular / Q000378:metabolism*; D015870:Gene Expression; D006084:Graft Rejection / Q000276:immunology; D006684:HLA-DR Antigens / Q000235:genetics; D016027:Heart Transplantation / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D013601:T-Lymphocytes / Q000502:physiology*; D014469:Umbilical Arteries / Q000166:cytology; D014471:Umbilical Veins / Q000166:cytology
D003470:Culture Media; D006684:HLA-DR Antigens; D010982:Platelet-Derived Growth Factor