Cell-free transmission of Vif mutants of HIV-1.
To determine the phenotype of human immunodeficiency virus type 1 (HIV-1) defective in the production of Vif, mutations were introduced into the vif gene of infectious molecular clones of the LAI, MAL, and ELI strains. Previous results had demonstrated that viruses derived from these wild type clones display different replicative capacities on peripheral blood mononuclear cells (PBMC) and different tropisms for CD4-positive cell lines. Using cell-free infection, Vif mutants of LAI, MAL, and ELI were found to have delayed kinetics and to produce less virus than their corresponding wild type viruses when propagated on most permissive cell lines. An additional mutation in the vpr gene in the LAI strain had no effect on this phenotype. However, on one T cell line, the H9 cell line, two Vif mutants of LAI and a Vif.Vpr double mutant were unable to replicate. Furthermore, Vif mutants from all three strains were only able to establish a productive infection on PBMC by coculture and not by cell-free infection. No defects in the processing of the virion proteins or the release of particles in the Vif mutants were detected.
Fan
L
L
Laboratory of Molecular Microbiology, NIAID, NIH, Bethesda, Maryland 20892.
Peden
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002474:Cell-Free System; D004279:DNA, Viral; D016346:Gene Products, vif / Q000235:genetics / Q000502:physiology*; D016341:Genes, vif; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D014162:Transfection; D054320:vif Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D016346:Gene Products, vif; D054320:vif Gene Products, Human Immunodeficiency Virus