Atrial natriuretic factor-like peptide and its prohormone within single cell organisms.
The present investigation was designed to determine if the atrial natriuretic peptide hormonal system is present within single cell organisms. Paramecium multimicronucleatum were examined with 3 sensitive and specific radioimmunoassays which recognize the N-terminus [amino acids 1-98; proANF(1-98)], the midportion of the N-terminus [amino acids 31-67; proANF(31-67)] and C-terminus (amino acids 99-126; ANF) of the 126 amino acid atrial natriuretic factor (ANF) prohormone. ProANF(1-98), proANF(31-67), and ANF-like peptides were all present within these unicellular organisms at concentrations of 460 +/- 19 pg/ml, 420 +/- 15 pg/ml, and 14.5 +/- 2 pg/ml, respectively. These concentrations are similar to their respective concentrations in the plasma of the rat (Rattus norvegicus). These results suggest that even single cell organisms contain the atrial natriuretic peptide-like hormonal system.
Vesely
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Department of Medicine, University of South Florida Health Sciences Center, Tampa 33612.
Giordano
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D000328:Adult; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010247:Paramecium / Q000737:chemistry*; D010446:Peptide Fragments / Q000276:immunology; D011498:Protein Precursors / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains
D010446:Peptide Fragments; D011498:Protein Precursors; C054548:atrial natriuretic factor prohormone (31-67); C055659:atrial natriuretic factor prohormone (1-98); D009320:Atrial Natriuretic Factor