IL-2-dependent generation of natural killer cells from bone marrow: role of MAC-1-, NK1-1- precursors.
We have previously shown that interleukin-2 (IL-2) is able to induce the generation of natural killer (NK) activity in bone marrow (BM) cell cultures from mice pretreated with 5-fluorouracil (5-FU). Cell fractionation experiments to analyze the nature of BM precursors indicate that MAC-1-, NK1-1- noncytotoxic precursors are induced by IL-2 to proliferate and generate cytolytic NK cells. These data demonstrate that the phenotype and functional characteristics of the IL-2-responsive cells in the FUBM are different from those of mature NK cells in that they are MAC-1+, NK1.1+, CD3- and susceptible to boosting by IFN-alpha.
Migliorati
G
G
Institute of Pharmacology, School of Medicine, University of Perugia, Italy.
Moraca
R
R
Nicoletti
I
I
Riccardi
C
C
D000818:Animals; D000941:Antigens / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000950:Antigens, Ly; D000954:Antigens, Surface; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D017252:CD3 Complex; D002454:Cell Differentiation; D005260:Female; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type; D016177:Macrophage-1 Antigen / Q000032:analysis; D008297:Male; D051379:Mice; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins / Q000032:analysis; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D014407:Tumor Cells, Cultured
D000941:Antigens; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface; D017252:CD3 Complex; D007376:Interleukin-2; C527211:Klrb1c protein, mouse; D037181:Lectins, C-Type; D016177:Macrophage-1 Antigen; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins; D011948:Receptors, Antigen, T-Cell