Translation enhancing properties of the 5'-leader of potato virus X genomic RNA.
The double-stranded DNA copy corresponding to the 5'-nontranslated alpha beta-leader of potato virus X (PVX) genomic RNA (positions -3 to-85 according to AUG initiator) was chemically synthesized and fused to the transcription plasmids containing three different reporter genes: neomycinphosphotransferase type II (NPT II) gene, Bacillus thuringiensis coleopteran-specific toxic protein gene and beta-glucuronidase (GUS) gene. Expression of the reporter genes in vitro and in plant protoplasts (in the case of GUS gene) reveals that the alpha beta-leader of PVX RNA acts as a translation enhancer despite the presence of the upstream vector-derived sequence and irrespective of the length of the spacer sequence preceding the reporter genes.
Zelenina
D A
DA
Department of Virology, Moscow State University, USSR.
Kulaeva
O I
OI
Smirnyagina
E V
EV
Solovyev
A G
AG
Miroshnichenko
N A
NA
Fedorkin
O N
ON
Rodionova
N P
NP
Morozov SYu
Atabekov
J G
JG
D000818:Animals; D001483:Base Sequence; D002474:Cell-Free System; D003001:Cloning, Molecular; D003062:Codon; D004279:DNA, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D011993:Recombinant Fusion Proteins; D011198:Solanum tuberosum / Q000382:microbiology
D003062:Codon; D004279:DNA, Viral; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins