A new biological activity of the complement factor H: identification of the precursor of the major macrophage-chemotactic factor in delayed hypersensitivity reaction sites of guinea pigs.
The guinea pig complement factor H(FH) and the plasma precursor(PMCFS-1) of the major monocyte-chemotactic factor(MCFS-1) found in the skin site of delayed hypersensitivity reaction(DHR) induced in the guinea pigs were compared in the antigenicity and the function. Both anti-FH-IgG and anti-MCFS-1-IgG formed a single precipitation line against FH, PMCFS-1, MCFS-1 and guinea pig plasma, and these lines fused one another without any spur formation. The inhibition activity of FH for C3bBb was absorbed by anti-MCFS-1-F(ab')2 in a dose-dependent manner. PMCFS-1 inhibited C3bBb activity dose-dependently as FH. These results show that FH is identical to PMCFS-1 and imply that FH, converted to MCFS-1 plays as a monocyte-chemotactic factor in the site of DHR.
Imamura
T
T
Department of Neuroscience and Immunology, Kumamoto University Graduate School of Medical Sciences, Japan.
Ohtsuka
H
H
Matsushita
M
M
Tsuruta
J
J
Okada
H
H
Kambara
T
T
D000818:Animals; D002630:Chemotactic Factors / Q000276:immunology / Q000502:physiology*; D002634:Chemotaxis, Leukocyte; D003180:Complement C3b Inactivator Proteins / Q000276:immunology / Q000502:physiology*; D017242:Complement Factor H; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000503:physiopathology*; D005779:Immunodiffusion; D008264:Macrophages / Q000502:physiology*; D011498:Protein Precursors / Q000276:immunology
D002630:Chemotactic Factors; D003180:Complement C3b Inactivator Proteins; D011498:Protein Precursors; D017242:Complement Factor H