Effects of oestrogen on mitochondrial thymidine kinase activity in the immature rat uterus.
Thymidine kinase (TK) is a key enzyme in the pyrimidine salvage pathway. High TK activity and the existence of cytosolic isozyme have consistently been observed in rapidly proliferating tissues. Oestrogen treatment induces the moderate increase of activities of mitochondrial TK and the fetal type TK isozyme in immature rat uterus. These results indicate that oestrogen induces the increase of uterine DNA synthesis in both cytosol and mitochondria in immature rats.
Sakamoto
S
S
Medical Research Institute, Tokyo Medical and Dental University, Japan.
Sawasaki
Y
Y
Kudo
H
H
Suzuki
S
S
Sawaki
K
K
Nagasawa
H
H
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D003600:Cytosol / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D003845:Deoxycytosine Nucleotides / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007527:Isoenzymes / Q000032:analysis*; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013937:Thymidine Kinase / Q000032:analysis*; D013942:Thymine Nucleotides / Q000494:pharmacology; D014599:Uterus / Q000187:drug effects* / Q000201:enzymology
D003845:Deoxycytosine Nucleotides; D007527:Isoenzymes; D013942:Thymine Nucleotides; C024107:2'-deoxycytidine 5'-triphosphate; D004958:Estradiol; D013937:Thymidine Kinase; C024157:thymidine 5'-triphosphate