title: Activating effect of adenosine on rat erythrocyte glycolysis.
abstract: 1. Adenosine increases the adenine nucleotide pool in rat erythrocytes. Hence, we tested the effect of the nucleoside on the glycolytic pathway in red blood cells. 2. A 2.5-fold increase in the level of fructose-1,6-bisphosphate and a 34% augmentation in lactate pool were observed in rat erythrocytes, 30 min after adenosine treatment. 3. Under conditions preventing adenosine metabolism, 1 microM nucleoside addition to isolated erythrocytes induced an 89% increase in lactate production and an increase in glucose consumption. 4. Activation of red cell phosphofructokinase (PFK) is produced by addition of microM concentrations of adenosine. Our data suggest a role for adenosine in the glycolysis flux regulation through PFK activation.
authors.affiliation: Departamento de Bioquimica, Facultad de Medicina, Universidad Nacional Autonóma de México, D.F.
authors.forename: R
authors.initials: R
authors.lastname: Gutierrez-Juarez
authors.forename: M
authors.initials: M
authors.lastname: Castrejon-Sosa
authors.forename: H
authors.initials: H
authors.lastname: Martinez-Valdez
authors.forename: P G
authors.initials: PG
authors.lastname: Blancas-Torres
authors.forename: E
authors.initials: E
authors.lastname: Piña
authors.forename: V
authors.initials: V
authors.lastname: Madrid-Marina
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D004912:Erythrocytes / Q000378:metabolism*; D005635:Fructosediphosphates / Q000097:blood; D006019:Glycolysis / Q000187:drug effects*; D007700:Kinetics; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008297:Male; D010732:Phosphofructokinase-1 / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005635:Fructosediphosphates; D007773:Lactates; D019344:Lactic Acid; D010732:Phosphofructokinase-1; D000241:Adenosine; C029063:fructose-1,6-diphosphate