T cell receptor V beta usage in synovial fluid of children with arthritis.
We have examined synovial fluid (SF) T cells from 5 children with various types of juvenile arthritis. Using the polymerase chain reaction (PCR) to determine the expression of 20 individual T cell receptor (TCR) beta variable (V) region families, we have shown with each patient that the SF T cells are polyclonal. Nevertheless, expanded usage of some families by SF T cells relative to peripheral blood T cells was evident in some cases. We conclude that the pattern of T lymphocytes in the inflamed joint may be a consequence in part of a polyclonal expansion or nonspecific homing of T cells to the joint, and in part a more restricted expansion of T cells potentially driven by arthritogenic antigens.
Doherty
P J
PJ
Department of Immunology, Toronto Western Hospital, ON, Canada.
Silverman
E D
ED
Laxer
R M
RM
Yang
S X
SX
Pan
S
S
D000974:Antibodies, Antinuclear / Q000032:analysis; D001171:Arthritis, Juvenile / Q000097:blood / Q000378:metabolism*; D002648:Child; D005260:Female; D015796:HLA-B27 Antigen / Q000032:analysis; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism*; D013582:Synovial Fluid / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism
D000974:Antibodies, Antinuclear; D015796:HLA-B27 Antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta