title: Thawing fresh frozen plasma in a microwave oven. A comparison with thawing in a 37 degrees C waterbath.
abstract: We show in this report that fresh frozen plasma (FFP) can be thawed faster using a specifically designed microwave oven (MWO) (WesLabs Plasma Defroster, Westmorland Laboratories, Inc., New Brunswick, Canada) than using 37 degrees C water bath (WB) and that the thawed product was equivalent to FFP thawed by WB. Paired plasma bags (200 mL/bag) from plasma pools were frozen, stored at -35 degrees C, and thawed in parallel, one bag in MWO, the other in WB. Mean thaw time (mean + SD) by MWO was 6.99 + 1.3 minutes; by WB the time was 17.6 + 1.7 minutes (n = 24; P less than 0.005). Rapid calorimetry of thawed plasma showed that MWO-thawed FFP temperature was 20.4 + 2.5 degrees C, whereas WB-thawed FFP was 15.4 + 3.3 degrees C (n = 24; P less than 0.005). Except for thrombin time (MWO = 20.1 seconds; WB = 19.8 seconds; n = 24; P = 0.023), no significant differences were observed in the 23 other coagulation parameters and plasma proteins studied. Faster thawing and freedom from risk of contamination may make MWO the method of choice for emergency thawing of FFP.
authors.affiliation: Blood Bank, Brigham Women's Hospital, Boston, Massachusetts 02115.
authors.forename: W H
authors.initials: WH
authors.lastname: Churchill
authors.forename: B
authors.initials: B
authors.lastname: Schmidt
authors.forename: J
authors.initials: J
authors.lastname: Lindsey
authors.forename: M
authors.initials: M
authors.lastname: Greenberg
authors.forename: S
authors.initials: S
authors.lastname: Boudrow
authors.forename: C
authors.initials: C
authors.lastname: Brugnara
mesh_terms: D001777:Blood Coagulation; D002151:Calorimetry; D005615:Freezing; D006801:Humans; D007101:Immersion; D008872:Microwaves; D010949:Plasma; D013048:Specimen Handling; D013696:Temperature; D013997:Time Factors