title: Isolation of human colonic crypts for study of xenobiotics metabolism.
abstract: Methods allowing study of human colonic drug and carcinogen metabolism in intact epithelial preparations in vitro are needed. We describe an isolation method yielding intact colonic crypts with well-maintained ultrastructural integrity and good viability parameters. The crypt preparations obtained with this method were used to study phase I and II drug biotransformation. The colonic crypts demethylated aminopyrine and nitrosodimethylamine slowly, but the colonic carcinogen dimethylhydrazine at a higher rate. The phenolic compounds estrone, 17 beta-estradiol, and 1-naphthol were readily conjugated. In conclusion, the present method yields preparations well suited for further investigation of the role of human colonic biotransformation in carcinogenesis and drug pharmacokinetics.
authors.affiliation: Abteilung Allgemeine Pharmakologie, Medizinische Hochschule Hannover, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schwenk
authors.forename: P
authors.initials: P
authors.lastname: Déchelotte
authors.forename: T
authors.initials: T
authors.lastname: Riemenschneider
mesh_terms: D000328:Adult; D000368:Aged; D001711:Biotransformation; D002273:Carcinogens / Q000378:metabolism; D003106:Colon / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D010101:Oxygen Consumption; D013048:Specimen Handling; D014529:Uridine / Q000378:metabolism; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D002273:Carcinogens; D015262:Xenobiotics; D014529:Uridine