title: Inhibition of polyamine synthesis impairs the secretion of atrial natriuretic peptide.
abstract: The aim of the present study was to evaluate in vivo the role of polyamines in the secretion of atrial natriuretic peptide (ANP). alpha-Difluoromethylornithine (DFMO) which inhibits ornithine decarboxylase activity and polyamine synthesis was given in drinking water and through intraperitoneal administration to Sprague-Dawley rats. Carotid artery was cannulated for collection of blood samples and measurement of blood pressure following the administration of arginine-vasopressin (AVP). Analysis of polyamines in cardiac tissue indicated that DFMO treatment decreased contents of putrescine and spermidine in cardiac tissue by 80% and 48%, respectively. Quantitation of ANP in plasma by radioimmunoassay indicated that both basal and stimulated levels of ANP in DFMO-treated animals were 21.5% and 50% of those in control rats. The administration of putrescine restored the levels of basal and AVP-stimulated levels of ANP in plasma which confirmed that DFMO effect on ANP secretion occurred specifically through the polyamine pathway.
authors.affiliation: Department of Pathology, University of Texas Medical Branch, Galveston 77550.
authors.forename: U R
authors.initials: UR
authors.lastname: Tipnis
authors.forename: P J
authors.initials: PJ
authors.lastname: Boor
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000008:administration & dosage / Q000494:pharmacology*; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects; D000518:Eflornithine / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013095:Spermidine / Q000378:metabolism
chemical_list: D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines; D001127:Arginine Vasopressin; D009320:Atrial Natriuretic Factor; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine