In-vitro interaction between nitrofurantoin and Vibrio cholerae DNA.
In-vitro interaction of nitrofurantoin with V. cholerae DNA resulted in a quenching and red spectral shift of the drug absorption pattern. Scatchard analysis revealed that the drug binding involved more than one processes and that the strongest mode of binding was characterised by an association constant (k) of 5.04 x 10(6) M-1 and the number of binding sites per nucleotide (n) of 0.015. Based on viscosity measurements, the mode of drug binding to DNA appeared to be through intercalation, the helix unwinding angle of supercoiled plasmid pBR322 DNA being 10 degrees. Nitrofurantoin binding to DNA resulted in an elevation of the thermal melting temperature (Tm) of DNA by 6 degrees C and inhibition of the action of DNase on DNA.
Mukherjee
U
U
Biophysics Division, Saha Institute of Nuclear Physics, Calcutta, India.
Chatterjee
S N
SN
D001665:Binding Sites; D004269:DNA, Bacterial / Q000737:chemistry* / Q000187:drug effects; D003851:Deoxyribonucleases / Q000737:chemistry; D004347:Drug Interactions; D007364:Intercalating Agents / Q000737:chemistry; D009582:Nitrofurantoin / Q000737:chemistry*; D013053:Spectrophotometry; D013696:Temperature; D013816:Thermodynamics; D014734:Vibrio cholerae / Q000737:chemistry* / Q000235:genetics; D014783:Viscosity
D004269:DNA, Bacterial; D007364:Intercalating Agents; D009582:Nitrofurantoin; D003851:Deoxyribonucleases