title: Biopsy specimen identification by detection of sex chromosomes: application of in situ hybridisation.
abstract: OBJECTIVE
  To investigate the feasibility of non-radioactive in situ hybridisation (ISH) for the identification of sex-mismatched plastic embedded bone marrow biopsy specimens.
  
  
  METHODS
  After a suspected accidental transposition of two glycol-methacrylate embedded bone marrow specimens, in situ hybridisation with sex chromosome specific probes was performed.
  
  
  RESULTS
  Quantitative analysis of the hybridisation signals established unequivocably the origin of the specimens.
  
  
  CONCLUSIONS
  ISH is feasible on GMA embedded bone marrow specimens, and can be used for the identification of accidentally transposed specimens provided that they are of sex-matched origin.
authors.affiliation: Laboratory of Pathology, State University Leiden, The Netherlands.
authors.forename: R E
authors.initials: RE
authors.lastname: Kibbelaar
authors.forename: C F
authors.initials: CF
authors.lastname: Leenheers-Binnendijk
authors.forename: P J
authors.initials: PJ
authors.lastname: Spaander
authors.forename: P M
authors.initials: PM
authors.lastname: Kluin
mesh_terms: D001853:Bone Marrow / Q000648:ultrastructure*; D003584:Cytological Techniques; D015342:DNA Probes; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012730:Sex Chromosomes / Q000737:chemistry*; D013048:Specimen Handling
chemical_list: D015342:DNA Probes