Analysis of K99 plasmids from enterotoxigenic Escherichia coli.
Evaluation of 9 wild-type K99 positive strains of Escherichia coli showed that each had a plasmid of approximately 87.8 kb that hybridized with two DNA probes specific for K99 genes. The K99 reference plasmid from E. coli also is 87.8 kb. Each of these strains had a conserved 7.15-kb BamHI fragment that also hybridized to these probes. Several K99 negative mutants and three 3P- strains also contained K99 plasmids as well as the 7.15-kb BamHI fragment. These results suggest that there is a conservation in size of the K99 plasmids of diverse strains.
Isaacson
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Department of Veterinary Pathobiology, University of Illinois, Urbana 61802.
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D001345:Autoradiography; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000032:analysis; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D010957:Plasmids; D013045:Species Specificity
D004269:DNA, Bacterial; D004768:Enterotoxins