title: Revised nucleotide sequence of the gltP gene, which encodes the proton-glutamate-aspartate transport protein of Escherichia coli K-12.
abstract: The gene encoding the proton-glutamate carrier (GltP) of Escherichia coli K-12 was sequenced, and the primary structure of the protein was analyzed. The nucleotide sequence was found to differ in several aspects from the previously published sequence (B. Wallace, Y. Yang, J. Hong, and D. Lum, J. Bacteriol. 172:3214-3220, 1990). The corrected open reading frame encodes a protein of 437 (instead of 395) amino acids. Hydropathy analysis predicts 12 membrane-spanning alpha-helical regions. The complementary strand does contain an open reading frame possibly encoding a highly hydrophilic polypeptide of 272 amino acids.
authors.affiliation: Department of Microbiology, University of Groningen, Haren, The Netherlands.
authors.forename: B
authors.initials: B
authors.lastname: Tolner
authors.forename: B
authors.initials: B
authors.lastname: Poolman
authors.forename: B
authors.initials: B
authors.lastname: Wallace
authors.forename: W N
authors.initials: WN
authors.lastname: Konings
mesh_terms: D000595:Amino Acid Sequence; D027322:Amino Acid Transport System X-AG; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005971:Glutamates / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012995:Solubility; D027981:Symporters
chemical_list: D027322:Amino Acid Transport System X-AG; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D005971:Glutamates; D006023:Glycoproteins; D027981:Symporters; C073766:GltP protein, E coli