Control of biosynthesis and post-transcriptional modification of the progesterone receptor.
The rabbit progesterone receptor undergoes dual regulation at the level of transcription: positive by estrogens and negative by progestins. The two aspects of this regulation are mediated by a single intragenic estrogen-responsive element. Estrogen receptor binding to this element has been demonstrated but progestin down-regulation does not proceed through DNA binding of the progesterone receptor. This result suggests some kind of protein-protein interaction--direct or indirect--between estrogen and progesterone receptors. At the post-transcriptional level, the progesterone receptor undergoes a hormone-dependent hyperphosphorylation of serine residues localized in the N-terminal region. Studies of progesterone receptor mutants have determined the influence of the different receptor domains in the phosphorylation mechanism. A casein kinase copurifies with the receptor. The role of this phosphorylation remains to be determined.
Chauchereau
A
A
Unité de Recherches Hormones et Reproduction, Institut National de la Santé et de la Recherche Médicale, (INSERM Unité 135), Hôpital de Bicêtre, France.
Savouret
J F
JF
Milgrom
E
E
D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D009154:Mutation; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011980:Receptors, Progesterone / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D011980:Receptors, Progesterone; D004247:DNA