Time course and inhibition of saponin-induced hemolysis.
Hemolytic activities of 3 steroid saponins reached plateaus within 5 min, whereas those of 4 triterpenoid saponins did not within 60 min. Erythrocytes pretreated with a low concentration of tigogenin were resistant to hemolysis of some of these saponins, but those pretreated with hecogenin or tomatidine were as sensitive as non-treated erythrocytes. Therefore, the ketone group of hecogenin or the amino group of tomatidine would weaken the interactions between the erythrocytes and these sapogenins. Furthermore, incubations of these saponins with a small amount of cholesterol diminished the hemolytic activities.
Takechi
M
M
Faculty of Pharmaceutical Sciences, Kinki University, Higashiosaka, Japan.
Shimada
S
S
Tanaka
Y
Y
D000818:Animals; D004912:Erythrocytes / Q000187:drug effects; D006460:Hemolysin Proteins; D066298:In Vitro Techniques; D008055:Lipids / Q000494:pharmacology; D012502:Sapogenins / Q000494:pharmacology; D012503:Saponins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D012756:Sheep; D013997:Time Factors
D006460:Hemolysin Proteins; D008055:Lipids; D012502:Sapogenins; D012503:Saponins