title: Differential expression of vimentin in rat prostatic tumors.
abstract: Differential hybridization analysis was used to identify molecular differences between a relative benign and a highly aggressive rat prostatic tumor derived from the Dunning R-3327-H adenocarcinoma. From the several differentially expressed mRNAs identified, we here report the characterization of pBUS51 which encodes a transcript highly expressed in all-anaplastic Dunning tumors. Only a very low expression was detectable in normal rat prostate or in the differentiated tumors of the Dunning system. Nucleotide sequence analysis and computer-assisted database comparison revealed that pBUS51 was highly homologous to vimentin and therefore likely the rat homolog of this protein.
authors.affiliation: Department of Urology, Urological Research Laboratory, University of Nijmegen, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: Bussemakers
authors.forename: G W
authors.initials: GW
authors.lastname: Verhaegh
authors.forename: A
authors.initials: A
authors.lastname: van Bokhoven
authors.forename: F M
authors.initials: FM
authors.lastname: Debruyne
authors.forename: J A
authors.initials: JA
authors.lastname: Schalken
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011471:Prostatic Neoplasms / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D015183:Restriction Mapping; D014746:Vimentin / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D014746:Vimentin