Inhibition of human immunodeficiency virus type 1 Rev function by a Rev mutant which interferes with nuclear/nucleolar localization of Rev.
A nonfunctional mutant of human immunodeficiency virus type 1 Rev was created by deleting seven amino acid residues within the nucleolar targeting signal. This mutant Rev remained in the cytoplasm in expressed cells and strongly inhibited the function of Rev by interfering with the nuclear/nucleolar localization of coexpressed Rev. These findings strongly suggest the multimerization of Rev in the cytoplasm before migration to the nucleus/nucleolus, where wild-type Rev functions as a trans-regulator.
Kubota
S
S
Department of Molecular Virology, Kyoto University, Japan.
Furuta
R
R
Maki
M
M
Hatanaka
M
M
D000595:Amino Acid Sequence; D001692:Biological Transport; D002460:Cell Line; D002466:Cell Nucleolus / Q000382:microbiology*; D002467:Cell Nucleus / Q000382:microbiology*; D015694:Gene Products, rev / Q000235:genetics / Q000378:metabolism*; D015757:Genes, rev; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D054321:rev Gene Products, Human Immunodeficiency Virus