Titration calorimetric and differential scanning calorimetric studies of the interactions of n-butanol with several phases of dipalmitoylphosphatidylcholine.
The interactions of n-butanol with dipalmitoylphosphatidylcholine (DPPC) were studied using titration calorimetry and differential scanning calorimetry (DSC). DSC results indicated that n-butanol induces the interdigitated phase in DPPC above 10 mg/mL butanol. A new application of titration calorimetry for measuring partition coefficients of nonsaturating solutes into lipids was developed. The partition coefficients and the heat of binding of n-butanol into DPPC were measured for the L beta', P beta', L alpha, and L beta I phases of DPPC. The partition coefficients were temperature dependent and ranged from 70 to 110 for the L beta I phase, from 170 to 183 for the L alpha phase, and similar to that for the L beta I phase in the P beta' phase. The binding to the L beta' phase could not be detected, giving an upper limit for this partition coefficient of 23. The enthalpies for binding to the L beta I and L alpha phases were 1.0 and 1.5 kcal/mol, respectively. The van't Hoff enthalpy was in good agreement with the calorimetric enthalpy for the partitioning into the L alpha phase; however, it was greater than the calorimetric enthalpy for the L beta I phase, suggesting that the interaction of n-butanol with this phase is cooperative in some way.
Zhang
F
F
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66103.
Rowe
E S
ES
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000440:Butanols / Q000737:chemistry*; D002151:Calorimetry / Q000379:methods; D002152:Calorimetry, Differential Scanning; D006358:Hot Temperature; D013816:Thermodynamics
D000440:Butanols; D015060:1,2-Dipalmitoylphosphatidylcholine