title: Trypsin-like arginine amidases including plasminogen and plasmin in human seminal plasma by affinity adsorption and elution.
abstract: An enzyme preparation with affinity to a lysine column was detected from a DEAE-cellulose-adsorbed preparation of human seminal plasma containing plasminogen and plasmin. Two kinds of trypsin-like acidic arginine amidase activity with different affinity to lima bean trypsin inhibitor (LBTI) and aprotinin affinity column were detected from the DEAE-cellulose-adsorbed preparation after treatment of the lysine column. Two kinds of trypsin-like basic arginine amidase activity were also separated by the above-mentioned affinity adsorptions from a CM-cellulose-adsorbed preparation of human seminal plasma. The effect of calcium chloride on these two enzymes was different from human acrosin.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: J Y
authors.initials: JY
authors.lastname: Park
authors.forename: I
authors.initials: I
authors.lastname: Hara
authors.forename: S
authors.initials: S
authors.lastname: Kaneko
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: S
authors.initials: S
authors.lastname: Nozawa
authors.forename: S
authors.initials: S
authors.lastname: Akihama
mesh_terms: D000327:Adsorption; D000581:Amidohydrolases / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D001120:Arginine / Q000378:metabolism*; D002122:Calcium Chloride; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012661:Semen / Q000201:enzymology*
chemical_list: D001120:Arginine; D000581:Amidohydrolases; C019665:amidase; D002122:Calcium Chloride