title: The effects of OKT4A monoclonal antibody on cellular immunity of nonhuman primate renal allograft recipients.
abstract: Significant differences in cellular responses were found among allograft recipients treated with various OKT4A mAb protocols. Recipients of multiple infusion low-dose and 2-bolus OKT4A immunosuppressive regimens regularly showed potent donor-specific cytotoxic CD8+ and CD4+ intragraft T cells and donor-reactive PBMC in MLC tests. In contrast, PBMC isolated from recipients of high-dose OKT4A therapy generally showed very weak or no response to donor-antigens during the later posttransplant periods. Furthermore, an absence of IL2-responsive intragraft cells was found to correlate with stable graft function in these recipients. We conclude that OKT4A mAb, in high doses, can block allosensitization and induce donor-specific nonresponsiveness in vivo. An OKT4A-based therapy, therefore, may have the potential of inducing long-lasting donor-specific immunosuppression, or even tolerance.
authors.affiliation: Transplantation Unit, Massachusetts General Hospital, Boston 02214.
authors.forename: S L
authors.initials: SL
authors.lastname: Wee
authors.forename: D M
authors.initials: DM
authors.lastname: Stroka
authors.forename: F I
authors.initials: FI
authors.lastname: Preffer
authors.forename: L K
authors.initials: LK
authors.lastname: Jolliffe
authors.forename: R B
authors.initials: RB
authors.lastname: Colvin
authors.forename: A B
authors.initials: AB
authors.lastname: Cosimi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002465:Cell Movement; D007111:Immunity, Cellular; D007166:Immunosuppressive Agents / Q000592:standards; D007376:Interleukin-2 / Q000494:pharmacology; D016030:Kidney Transplantation / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008252:Macaca fascicularis; D008297:Male; D014184:Transplantation, Homologous / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016827:CD8 Antigens; D007166:Immunosuppressive Agents; D007376:Interleukin-2