Sequence of the vanC gene of Enterococcus gallinarum BM4174 encoding a D-alanine:D-alanine ligase-related protein necessary for vancomycin resistance.
The amplification product obtained with DNA from vancomycin-resistant (VmR) Enterococcus gallinarum BM4174 and a pair of degenerate oligodeoxyribonucleotides that correspond to conserved amino acid (aa) motifs in Escherichia coli D-alanine (D-Ala):D-Ala ligases and in En. faecium VmR protein (VanA) was used as a probe to clone the vanC gene of that strain. The vanC product, with a calculated Mr of 37,504, exhibits 29 to 38% aa identity with VanA and E. coli ligases. Insertional inactivation of vanC led to Vm sensitivity of BM4174 suggesting that the gene may encode a D-Ala:D-Ala ligase of altered specificity.
Dutka-Malen
S
S
Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
Molinas
C
C
Arthur
M
M
Courvalin
P
P
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D004252:DNA Mutational Analysis; D004351:Drug Resistance / Q000235:genetics; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000737:chemistry / Q000235:genetics*; D014640:Vancomycin / Q000494:pharmacology*
D001426:Bacterial Proteins; D014640:Vancomycin; D010453:Peptide Synthases; C019638:D-alanylalanine synthetase