Localization of basic fibroblast growth factor-like immunoreactivity in the rat brain.
The immunohistochemical localization of basic fibroblast growth factor (bFGF) was studied in the adult rat brain, using a specific antibody against a synthetic bFGF fragment (the N-terminal 12 residues). Widespread but uneven regional localization of bFGF-like immunoreactive neurons and fibers was observed. Ependymal cells were also stained. The immunoreactive neurons were found in the cerebral cortex, olfactory bulb, septum, basal magnocellular nuclei, thalamus, hypothalamus, globus pallidus, hippocampus, amygdala, red nucleus, central gray of the midbrain, cerebellum, dorsal tegmental area, reticular formation, cranial motor nuclei and spinal cord. Immunoreactive fiber bundles and nerve terminals were also detected. These results indicate that bFGF is produced by or present in a specific neuronal cell population of the central nervous system.
Matsuyama
A
A
Department of Neuroanatomy, Osaka University Medical School, Japan.
Iwata
H
H
Okumura
N
N
Yoshida
S
S
Imaizumi
K
K
Lee
Y
Y
Shiraishi
S
S
Shiosaka
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001921:Brain / Q000033:anatomy & histology*; D001923:Brain Chemistry / Q000502:physiology*; D001933:Brain Stem / Q000033:anatomy & histology / Q000276:immunology / Q000378:metabolism; D002531:Cerebellum / Q000033:anatomy & histology / Q000276:immunology / Q000378:metabolism; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000276:immunology / Q000378:metabolism; D003429:Cross Reactions; D016222:Fibroblast Growth Factor 2 / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008032:Limbic System / Q000033:anatomy & histology / Q000276:immunology / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009411:Nerve Endings / Q000276:immunology / Q000378:metabolism; D009412:Nerve Fibers / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000033:anatomy & histology / Q000276:immunology / Q000378:metabolism
D016222:Fibroblast Growth Factor 2