Identification of a gp63 surface glycoprotein in Leishmania tarentolae.
The promastigote stage of most if not all Leishmania species possesses an abundant surface glycoprotein of 63 kDa (gp63) that has protease activity. We show that the lizard parasite Leishmania tarentolae appears to lack the surface protease activity. L. tarentolae does, however, possess an approximately 63-kDa molecule that is antigenically cross-reactive with the L. major gp63. Additionally, the genome of L. tarentolae contains sequences that hybridise at high stringency to a L. major gp63 gene probe.
Campbell
D A
DA
Department of Microbiology and Immunology, University of California, Los Angeles 90024.
Kurath
U
U
Fleischmann
J
J
D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D003429:Cross Reactions; D016054:DNA, Protozoan / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D007891:Leishmania / Q000737:chemistry* / Q000235:genetics; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D008666:Metalloendopeptidases / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid
D016054:DNA, Protozoan; D008562:Membrane Glycoproteins; D010450:Endopeptidases; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania