title: The 'natural' hybrid haemoglobin from mule. Interrelationships with its parent haemoglobins from horse and donkey.
abstract: The equilibrium O2-binding properties of the hybrid haemoglobin (Hb) present in vivo in erythrocytes from mule and of its parent Hbs from horse and donkey were compared with special reference to the effect of heterotropic ligands such as Cl-, D-glycerate 2,3-bisphosphate (DPG) and inositol hexakisphosphate. All these Hbs display a decreased effect by polyphosphates, confirming that what has been observed for horse Hb [Giardina, Brix, Clementi, Scatena, Nicoletti, Cicchetti, Argentin & Condò (1990) Biochem. J. 266, 897-900] is common to other equine species, at least from a qualitative standpoint. However, different quantitative aspects can be detected, which can be accounted for by a different role for the two types of chain in characterizing the binding free energy for the various heterotropic effectors. In particular, it is shown that the binding mode of DPG and inositol hexakisphosphate displays different features since long-range effects can be observed clearly for inositol hexakisphosphate but not for DPG. In general terms, in spite of a different intrinsic O2 affinity, the modulation of functional properties by third ligands leads these Hbs to behave, under physiological conditions, similarly to human HbA. It might represent an interesting example of how different species with similar functional needs find different ways to produce a similar functional behaviour.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Italy.
authors.forename: S G
authors.initials: SG
authors.lastname: Condò
authors.forename: M
authors.initials: M
authors.lastname: Coletta
authors.forename: R
authors.initials: R
authors.lastname: Cicchetti
authors.forename: G
authors.initials: G
authors.lastname: Argentin
authors.forename: P
authors.initials: P
authors.lastname: Guerrieri
authors.forename: S
authors.initials: S
authors.lastname: Marini
authors.forename: S
authors.initials: S
authors.lastname: el-Sherbini
authors.forename: B
authors.initials: B
authors.lastname: Giardina
mesh_terms: D000818:Animals; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006736:Horses; D007526:Isoelectric Point; D010527:Perissodactyla / Q000097:blood*; D010833:Phytic Acid / Q000378:metabolism; D013045:Species Specificity
chemical_list: D004163:Diphosphoglyceric Acids; D006454:Hemoglobins; C015891:glycerate 1,3-biphosphate; D010833:Phytic Acid