Presence of angiotensin peptides in human urine.
Immunoreactive angiotensin I and angiotensin II were found in human urine that was purified on octadecasilylsilica cartridges. The daily excretion of angiotensin I and II in healthy volunteers was 189.00 (SE 38.36) and 17.54 (SE 3.07) pmol/24 h or 148.09 (SE 32.22) and 12.82 (SE 2.34) pmol/L, respectively (n = 12). No circadian rhythm was observed in the excretion patterns of angiotensin I and II. In vitro degradation of angiotensin I or II could not be detected in acidified urine samples. A marked increase in the excretion of angiotensin I and II could be demonstrated in patients with anaphylactoid reactions to drugs and food additives after oral challenge. Immunoreactive angiotensin I and II could be characterized by HPLC as Ile5-angiotensin I, Ile5-angiotensin II, and angiotensin II metabolites.
Hermann
K
K
Department of Dermatology, Ludwig Maximilians University, Munich, FRG.
Rittweger
R
R
Phillips
M I
MI
Ring
J
J
D000328:Adult; D000707:Anaphylaxis / Q000652:urine; D000803:Angiotensin I / Q000378:metabolism / Q000652:urine*; D000804:Angiotensin II / Q000378:metabolism / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay
D000804:Angiotensin II; D000803:Angiotensin I