Interaction between human cyclin A and adenovirus E1A-associated p107 protein.
The products of the adenovirus early region 1A (E1A) gene are potent oncoproteins when tested in standard transformation and immortalization assays. Many of the changes induced by E1A may be due to its interaction with cellular proteins. Four of these cellular proteins are the retinoblastoma protein (pRB), p107, cyclin A, and p33cdk2. The pRB and p107 proteins are structurally related and have several characteristics in common, including that they both bind to the SV40 large T oncoprotein as well as to E1A. Cyclin A and p33cdk2 are thought to function in the control of the cell cycle. They bind to one another, forming a kinase that closely resembles the cell cycle-regulating complexes containing p34cdc2. Cyclin A is now shown to bind to p107 in the absence of E1A. The association of p107 with cyclin A suggests a direct link between cell cycle control and the function of p107.
Faha
B
B
Massachusetts General Hospital Cancer Center, Charlestown 02129.
Ewen
M E
ME
Tsai
L H
LH
Livingston
D M
DM
Harlow
E
E
D017456:Adenovirus Early Proteins; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002460:Cell Line; D016213:Cyclins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D016160:Retinoblastoma Protein / Q000378:metabolism; D050720:Retinoblastoma-Like Protein p107
D017456:Adenovirus Early Proteins; D000911:Antibodies, Monoclonal; D016213:Cyclins; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; D011506:Proteins; C493844:RBL1 protein, human; D011993:Recombinant Fusion Proteins; D016160:Retinoblastoma Protein; D050720:Retinoblastoma-Like Protein p107; D008715:Methionine; D005982:Glutathione Transferase; D016203:CDC2 Protein Kinase