Identification of a major growth factor for AIDS-Kaposi's sarcoma cells as oncostatin M.
Conditioned medium from human T cell leukemia virus type 2 (HTLV-II)-infected T cells supports the growth and long-term culture of cells derived from acquired immunodeficiency syndrome (AIDS)-associated Kaposi's sarcoma lesions (AIDS-KS cells). A protein of 30 kilodaltons was purified from conditioned medium that supports the growth of AIDS-KS cells. The amino-terminal sequence of this protein was identical to the amino-terminal sequence of Oncostatin M, a glycoprotein that inhibits the growth of a variety of cancer cells. Oncostatin M from conditioned medium stimulated a twofold increase in the growth of AIDS-KS cells at a concentration of less than 1 nanogram of the protein per milliliter of medium.
Nair
B C
BC
Department of Cell Biology, Advanced BioScience Laboratories, Inc., Kensington, MD.
DeVico
A L
AL
Nakamura
S
S
Copeland
T D
TD
Chen
Y
Y
Patel
A
A
O'Neil
T
T
Oroszlan
S
S
Gallo
R C
RC
Sarngadharan
M G
MG
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000595:Amino Acid Sequence; D003470:Culture Media / Q000737:chemistry; D006133:Growth Substances / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D053683:Oncostatin M; D010455:Peptides / Q000302:isolation & purification / Q000502:physiology*; D012514:Sarcoma, Kaposi / Q000209:etiology / Q000473:pathology*; D014407:Tumor Cells, Cultured
D003470:Culture Media; D006133:Growth Substances; C508351:OSM protein, human; D010455:Peptides; D053683:Oncostatin M