Evaluation of four commercial methods for identification and biotyping of genital and neonatal strains of Haemophilus species.
Four commercial methods for identification of Haemophilus species were evaluated in comparison to conventional methods using 188 genital and neonatal Haemophilus strains. In the case of discrepancies between results obtained by the different methods, DNA-DNA hybridization was performed. The four commercial systems and conventional methods showed excellent correlation of results in 167 strains (88%). DNA-DNA hybridization was performed in 8 strains with discrepant identification results and 13 strains with discrepant biotyping results. In 15 cases discrepancies could be explained by the fact that the strains belonged to a newly recognised species of Haemophilus.
Quentin
R
R
Départment de Microbiologie Médicale et Moléculaire, URA CNRS 1334, Centre Hospitalier Universitaire Bretonneau, Tours, France.
Dubarry
I
I
Martin
C
C
Cattier
B
B
Goudeau
A
A
D004269:DNA, Bacterial / Q000032:analysis; D005069:Evaluation Studies as Topic; D005260:Female; D005831:Genital Diseases, Female / Q000382:microbiology; D006190:Haemophilus / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D009693:Nucleic Acid Hybridization
D004269:DNA, Bacterial