cDNA cloning for and preparation of antibodies against subunit d of H(+)-ATP synthase in rat mitochondria.
The cDNA for subunit d of rat mitochondrial H(+)-ATP synthase was cloned from a brain cDNA library. The protein contains internally repeat structures that have sequences similar to those of other ATP-related proteins. The antibodies raised against the protein A-subunit d fusion protein expressed in E. coli specifically recognized only the protein in the mitochondria. The Na2 CO3 fractionation followed by immunoblotting analyses suggest that at least a part of the protein is inserted into the membrane.
Motojima
K
K
Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Imanaka
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002994:Clofibrate / Q000494:pharmacology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D015151:Immunoblotting; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D011132:Polyribosomes / Q000187:drug effects / Q000502:physiology; D011487:Protein Conformation; D006180:Proton-Translocating ATPases / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000906:Antibodies; D046911:Macromolecular Substances; D004247:DNA; D006180:Proton-Translocating ATPases; D002994:Clofibrate