title: Non-essential repeats in the promoter region of a Brassica rapa acyl carrier protein gene expressed in developing embryos.
abstract: A genomic clone of an acyl carrier protein gene (Bcg4-4) which is highly expressed in developing embryos of Brassica rapa was isolated and sequenced. The promoter and transcription terminator regions of Bcg4-4 were used to express a beta-glucuronidase reporter gene in transgenic rapeseed. Deletion of repeated domains in the promoter region did not lower beta-glucuronidase expression in seeds.
authors.affiliation: Calgene, Inc., Davis, CA 95616.
authors.forename: D
authors.initials: D
authors.lastname: Scherer
authors.forename: A
authors.initials: A
authors.lastname: Sato
authors.forename: D W
authors.initials: DW
authors.lastname: McCarter
authors.forename: S E
authors.initials: SE
authors.lastname: Radke
authors.forename: J C
authors.initials: JC
authors.lastname: Kridl
authors.forename: V C
authors.initials: VC
authors.lastname: Knauf
mesh_terms: D000213:Acyl Carrier Protein / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017343:Genes, Plant; D015698:Genomic Library; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012639:Seeds / Q000502:physiology; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D000213:Acyl Carrier Protein; D004247:DNA; D005966:Glucuronidase