Specific binding of nucleotides and NAD+ to Clostridium difficile toxin A.
Binding of nucleotides, a tetrapolyphosphate, and NAD+ to purified toxin A of Clostridium difficile was determined by monitoring changes in intrinsic fluorescence following excitation at 280 nm, and recording emissions at 340 nm. Binding was specific for concentrations over the range 5 to 100 microM for ATP, GTP, and their respective non-hydrolysable analogues AMP-PNP and Gpp(NH)p, tetrapolyphosphate and NAD+.
Lobban
M D
MD
Department of Biochemistry, University of Glasgow, UK.
Borriello
S P
SP
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000378:metabolism*; D001665:Binding Sites; D016360:Clostridioides difficile / Q000378:metabolism*; D004768:Enterotoxins / Q000737:chemistry / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000737:chemistry
D001427:Bacterial Toxins; D004768:Enterotoxins; D009711:Nucleotides; C041348:tcdA protein, Clostridium difficile; D009243:NAD; D006160:Guanosine Triphosphate; D014364:Tryptophan; D000255:Adenosine Triphosphate