title: Increased endocytotic and lysosomal activities in denervated type I and type II muscle fibres.
abstract: Previous work has shown that increased endocytotic and lysosomal activities occur in the endplate region of denervated skeletal muscle fibres. This, however, does not engage all fibres of a muscle at a given time after denervation. The present study was carried out in order to determine if both type I (slow) and type II (fast) muscle fibres can react to denervation by increased endocytotic and lysosomal activities. Uptake of horseradish peroxidase as a marker for endocytosis was studied in conjunction with acid phosphatase staining for lysosomal activity in type I and type II fibres of the denervated mouse hemidiaphragm. Fibre typing was performed using a monoclonal antibody against fast skeletal myosin and by adenosine triphosphatase staining. The results show that increased endocytosis and lysosomal activation occur in both type I and type II fibres after denervation.
authors.affiliation: Department of Pharmacology, University of Lund, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Lawoko
authors.forename: S
authors.initials: S
authors.lastname: Tågerud
authors.forename: R
authors.initials: R
authors.lastname: Libelius
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D004705:Endocytosis; D005260:Female; D007150:Immunohistochemistry; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D051379:Mice; D009121:Muscle Denervation; D009132:Muscles / Q000378:metabolism*
chemical_list: D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases