title: Clonal diversity of methicillin-resistant Staphylococcus aureus in an acute-care institution.
abstract: OBJECTIVE
  To evaluate plasmid and chromosomal typing methods for differentiation of methicillin-resistant Staphylococcus aureus (MRSA).
  
  
  METHODS
  Comparison of relatedness of strains using epidemiologic features, phage typing, and antimicrobial susceptibility with blinded assessment by molecular typing methods. Molecular typing methods included AccI and ClaI restriction endonuclease fingerprinting of chromosomal DNA and plasmid typing.
  
  
  METHODS
  Tertiary-care teaching hospital.
  
  
  METHODS
  Convenience sample of 10 MRSA strains, including 4 outbreak-associated and 6 sporadic strains of diverse epidemiologic origins without evidence of nosocomial transmission.
  
  
  RESULTS
  Only 2 strains were phage typeable. The epidemic strain was distinguishable by its susceptibility pattern. The other strains were not consistently separable by phenotyping or plasmid typing methods but were distinct by chromosome typing.
  
  
  CONCLUSIONS
  These observations document the diversity of origins of MRSA strains introduced into an acute-care institution. Chromosomal typing may be the preferred method for the determination of clonal origin of MRSA.
authors.affiliation: Infection Control Unit, University of Manitoba, Winnipeg, Canada.
authors.forename: L E
authors.initials: LE
authors.lastname: Nicolle
authors.forename: H
authors.initials: H
authors.lastname: Bialkowska-Hobrzanska
authors.forename: L
authors.initials: L
authors.lastname: Romance
authors.forename: V S
authors.initials: VS
authors.lastname: Harry
authors.forename: S
authors.initials: S
authors.lastname: Parker
mesh_terms: D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000382:microbiology; D005260:Female; D006784:Hospitals, Teaching; D006801:Humans; D008297:Male; D008350:Manitoba; D016106:Methicillin Resistance / Q000235:genetics*; D010641:Phenotype; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification