title: Passive immunization protects guinea pigs from lethal Toxoplasma infection.
abstract: The cellular and humoral interactions that contribute to protective immunity in toxoplasmosis were studied by adoptive transfer of selective cell populations or immune serum and its fractions into normal syngeneic strain 2 guinea pigs. The results of this study with the RH strain of Toxoplasma gondii confirm and extend the findings of previous studies by showing that the passive transfer of parasite-sensitized T cells or of immune serum from previously infected donors protected recipient guinea pigs against lethal toxoplasmosis. An additional key finding was that similar levels of complete protection against lethal infection occurred in guinea pigs receiving partially purified anti-Toxoplasma immunoglobulins or immune cells that had been enriched for B cells prior to transfer. Cells residing in the spleen, lymph nodes and peritoneal cavity, but not the thymus, were equally effective in conferring immunity to challenged recipients. In addition, cell titration experiments revealed that guinea pigs could survive T. gondii infection by infusing them with as little as 2 x 10(7) sensitized T cells or B cells. Unlike protection mediated by T cells, protection against lethal disease occurring in the B cell recipients was associated with the formation of Toxoplasma antibodies. These findings illustrate the major role of both humoral and cell-mediated immunity in affording protection against toxoplasmosis based on a guinea pig model of the human disease.
authors.affiliation: Department of Medicine, New York Medical College, Valhalla.
authors.forename: C S
authors.initials: CS
authors.lastname: Pavia
authors.forename: S J
authors.initials: SJ
authors.lastname: Bittker
authors.forename: K E
authors.initials: KE
authors.lastname: Curnick
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D007116:Immunization, Passive; D013601:T-Lymphocytes / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000913:Antibodies, Protozoan; D007106:Immune Sera