title: Antibody response to Staphylococcus aureus whole cell, lipase and staphylolysin in patients with S. aureus infections.
abstract: Three assays to measure antibodies against Staphylococcus aureus whole cells, lipase and staphylolysin were used to try to discriminate between complicated and uncomplicated S. aureus septicaemia. Sera were examined from 8 patients with S. aureus endocarditis, 23 patients with complicated S. aureus septicaemia, 12 patients with uncomplicated S. aureus septicaemia and 93 febrile non-septicaemic controls. No single assay could distinguish between complicated and uncomplicated S. aureus septicaemia. If the criterion for a positive result is defined as positive antibody level in the anti-lipase ELISA as well as in at least 1 of the other 2 assays, 10/31 patients with S. aureus endocarditis or complicated septicaemia were positive compared to 0/93 non-septicaemic patients and 0/12 patients with uncomplicated S. aureus septicaemia. Therefore, the combined use of serological assays in the diagnosis of complicated S. aureus septicaemia, one of which is the anti-lipase ELISA, is recommended.
authors.affiliation: Department of Infectious Diseases, Lund University Hospital, Sweden.
authors.forename: U
authors.initials: U
authors.lastname: Ryding
authors.forename: J
authors.initials: J
authors.lastname: Renneberg
authors.forename: J
authors.initials: J
authors.lastname: Rollof
authors.forename: B
authors.initials: B
authors.lastname: Christensson
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D016470:Bacteremia / Q000175:diagnosis*; D003937:Diagnosis, Differential; D004697:Endocarditis, Bacterial / Q000175:diagnosis; D004731:Endotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008049:Lipase / Q000276:immunology*; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D004731:Endotoxins; C016110:staphylolysin; D008049:Lipase