title: Alterations in lipid peroxidation, antioxidant enzymes, and carcinogen metabolism in liver microsomes of vitamin E-deficient trout and rat.
abstract: Feeding rainbow trout for 16 weeks a diet in which the levels of vitamin E were reduced 70-fold resulted in marked depletion (18-fold) of vitamin E levels in liver microsomes from these fish. The susceptibility of hepatic microsomes to lipid peroxidation in vitro and the levels of plasma and liver microsomal lipid hydroperoxides generated in vivo were markedly elevated in vitamin E-depleted trout. No appreciable alterations were observed in the liver microsomal cytochrome P450-dependent mixed-function oxidase system or in the fatty acid composition of trout liver microsomal membranes. Livers from rats fed a vitamin E-deficient diet for 10 weeks also had significantly lower levels of microsomal vitamin E. In addition, total cytochrome P450 levels were depressed (15%) and cytosolic glutathione was enhanced (40%) in livers from rats fed the vitamin E-depleted diet. Covalent binding of [3H]-(+)-benzo[a]pyrene-7,8-dihydrodiol to exogenous DNA in vitro was enhanced with liver microsomes from vitamin E-deficient trout and these fish were much more sensitive to the acute toxicity of this carcinogenic polycyclic aromatic hydrocarbon. These results indicate that trout may be a useful model for studying the significance of peroxidative pathways in carcinogenesis and their manipulation by dietary antioxidants.
authors.affiliation: Department of Food Science & Technology, Oregon State University, Corvallis 67331.
authors.forename: D E
authors.initials: DE
authors.lastname: Williams
authors.forename: H M
authors.initials: HM
authors.lastname: Carpenter
authors.forename: D R
authors.initials: DR
authors.lastname: Buhler
authors.forename: J D
authors.initials: JD
authors.lastname: Kelly
authors.forename: M
authors.initials: M
authors.lastname: Dutchuk
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014337:Trout; D014811:Vitamin E Deficiency / Q000378:metabolism*; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D005227:Fatty Acids; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System