title: Recombinant Leishmania Hsp90 and Hsp70 are recognized by sera from visceral leishmaniasis patients but not Chagas' disease patients.
abstract: Approximately 70% of the cDNA clones identified by immunoscreening Leishmania donovani expression libraries with serum from a patient with visceral leishmaniasis (kala-azar) were found to encode the highly conserved Hsp90 and Hsp70 members of the heat shock protein family. Recombinant fusion proteins containing the C-terminal portions of L. donovani Hsp90 and Hsp70 were used as target antigens in enzyme-linked immunosorbent assays of various sera. Sera from four patients with visceral leishmaniasis recognized recombinant Leishmania Hsp90 and Hsp70, while sera from seven patients with Chagas' disease did not, despite the fact that Trypanosoma cruzi Hsp90 and Hsp70 share more than 80% amino acid identity with their counterparts in Leishmania spp. Thus, Leishmania Hsp90 and Hsp70 elicit strong humoral responses and are potential candidates for specific serodiagnostic assays capable of distinguishing between L. donovani and T. cruzi infections.
authors.affiliation: Department of Internal Medicine, University of Iowa, Iowa City.
authors.forename: C R
authors.initials: CR
authors.lastname: de Andrade
authors.forename: L V
authors.initials: LV
authors.lastname: Kirchhoff
authors.forename: J E
authors.initials: JE
authors.lastname: Donelson
authors.forename: K
authors.initials: K
authors.lastname: Otsu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014355:Chagas Disease / Q000276:immunology*; D016054:DNA, Protozoan / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007893:Leishmania donovani / Q000235:genetics* / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid; D014349:Trypanosoma cruzi / Q000235:genetics / Q000276:immunology
chemical_list: D016054:DNA, Protozoan; D006360:Heat-Shock Proteins; D011993:Recombinant Fusion Proteins