title: Characterization of the glyoxysomal isocitrate lyase genes of Aspergillus nidulans (acuD) and Neurospora crassa (acu-3).
abstract: The nucleotide sequences of the genes encoding the acetate-inducible glyoxylate cycle enzyme isocitrate lyase from the ascomycete fungi Aspergillus nidulans (acuD) and Neurospora crassa (acu-3) are presented. The respective A. nidulans and N. crassa genes are interrupted at identical positions by two introns and encode proteins of 538 and 543 amino acids, which have 75% identity. The predicted protein sequences do not demonstrate the C-terminal tripeptide S-K-L that has been implicated in peroxisomal targeting and found in the glyoxysomally located enzyme malate synthase from the same species. However, the protein sequences do exhibit a partial repeat which, in common with malate synthase, is located in regions that are absent from, or non-homologous with, the E. coli enzyme, which is not compartmentalized.
authors.affiliation: Department of Microbiology, University of Reading, Whiteknights, UK.
authors.forename: L D
authors.initials: LD
authors.lastname: Gainey
authors.forename: I F
authors.initials: IF
authors.lastname: Connerton
authors.forename: E H
authors.initials: EH
authors.lastname: Lewis
authors.forename: G
authors.initials: G
authors.lastname: Turner
authors.forename: D J
authors.initials: DJ
authors.lastname: Ballance
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D006038:Glyoxylates / Q000378:metabolism*; D007522:Isocitrate Lyase / Q000737:chemistry / Q000235:genetics*; D008292:Malate Synthase / Q000737:chemistry / Q000235:genetics; D008830:Microbodies / Q000201:enzymology*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004271:DNA, Fungal; D006038:Glyoxylates; D008292:Malate Synthase; D007522:Isocitrate Lyase