[Identification of sex in mouse preimplantation embryos by indirect immunofluorescent assay].
Indirect immunofluorescent assay was used for detecting male-specific H-Y antigen on preimplantation embryos. Our results showed that, embryos examined, 54.1% were H-Y positive (male) and 45.9% were H-Y negative (female). Significant difference was not found between natural sex ratio of kunming strain mouse (male: 52.1%; female: 47.9%) and our results (P greater than 0.05). Moreover, the results sequentially were confirmed by cytogenetic method. 75.50% H-Y positive embryos were male and 82.97% H-Y negative embryos were female respectively (P less than 0.01). Some factors influencing the accurency of sex selection with indirect immunofluorescent assay were also discussed.
Men
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H
Kunming Institute of Zoology, Academia Sinica.
Chen
Y
Y
Zhou
X
X
He
X
X
Shi
L
L
D000818:Animals; D001755:Blastocyst / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006182:H-Y Antigen / Q000032:analysis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D012732:Sex Determination Analysis
D006182:H-Y Antigen