The formation of metabolites of mephentermine by microsomal and cytosolic preparations of male Wistar rat livers.
1. Metabolites of mephentermine (MP), phentermine (Ph), p-hydroxy-MP, p-hydroxy-Ph, N-hydroxy-MP and N-hydroxy-Ph on incubation with rat liver microsomal and cytosolic preparations were identified by g.l.c. and g.l.c.-mass spectrometry. 2. Identification of the metabolites indicated the following new metabolic routes of MP: NADPH-dependent microsomal formation of p-hydroxy-MP from MP, of p-hydroxy-Ph from p-hydroxy-MP, and the NADH-dependent microsomal formation of Ph from N-hydroxy-Ph.
Mori
M A
MA
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Kobayashi
M
M
Sakai
K
K
Nakafuku
K
K
Mori
Y
Y
Kozuka
H
H
D000818:Animals; D003600:Cytosol / Q000378:metabolism*; D008658:Inactivation, Metabolic; D008099:Liver / Q000378:metabolism*; D008297:Male; D008616:Mephentermine / Q000031:analogs & derivatives / Q000378:metabolism / Q000493:pharmacokinetics*; D008862:Microsomes, Liver / Q000378:metabolism*; D008954:Models, Biological; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010645:Phentermine / Q000031:analogs & derivatives / Q000378:metabolism / Q000493:pharmacokinetics; D051381:Rats; D011919:Rats, Inbred Strains
D009243:NAD; C066111:4-hydroxymephentermine; C013568:N-hydroxyphentermine; C066112:4-hydroxyphentermine; D009249:NADP; C076661:N-hydroxymephentermine; D010645:Phentermine; D008616:Mephentermine