Suramin prevents import of acyl-CoA oxidase into rat liver peroxisomes.
The inhibitory effect of suramin on the import of [35S]acyl-CoA oxidase into purified rat liver peroxisomes was investigated in vitro. The import of acyl-CoA oxidase was inhibited completely by 10 microM suramin, whilst the latency of catalase remained unchanged. The important value decreased 60% by pretreatment of peroxisomes with 10 microM suramin, but it did not decrease by pretreatment of translation products. Polysulfonate compounds which have two clusters of negative charges, such as Cibacron blue F3GA and Trypan blue, as well as suramin, inhibited the import, whilst mono- and disulfonate compounds did not.
Imanaka
T
T
Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Lazarow
P B
PB
Takano
T
T
D042962:Acyl-CoA Oxidase; D000251:Adenosine Triphosphatases / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008830:Microbodies / Q000187:drug effects* / Q000201:enzymology; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D013498:Suramin / Q000494:pharmacology*
D013498:Suramin; D010088:Oxidoreductases; D002374:Catalase; D042962:Acyl-CoA Oxidase; D000251:Adenosine Triphosphatases