Similarity of the genomic structure between the two members in a new family of heparin-binding factors.
By differential hybridization of cDNA libraries from Fv-1 restrictive and non-restrictive somatic mutant mouse cells, we isolated a cDNA clone specifically present in the Fv-1 restrictive cells. Its sequence was found identical to that of heparin-binding growth-associated molecule or pleiotrophin (HB-GAM/PTN). We report here its genomic organization which has not been yet reported. Although the exon organization was similar to that of midkine, another member of the same family of heparin-binding factors, the sizes of introns were much larger and occupied more than a three-fold larger chromosomal region than midkine. As its introduction into Fv-1 non-restrictive cells failed to confer the Fv-1 restrictive character, HB-GAM/PTN is probably unrelated to Fv-1 restriction.
Naito
A
A
Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
Yoshikura
H
H
Iwamoto
A
A
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins; D002460:Cell Line; D003001:Cloning, Molecular; D016207:Cytokines / Q000145:classification / Q000235:genetics*; D005347:Fibroblasts; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000145:classification / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D002352:Carrier Proteins; D016207:Cytokines; D011506:Proteins; C066845:pleiotrophin