Construction of an infectious cDNA clone of echovirus 6.
A complete cDNA copy of the echovirus 6 genome was constructed. Complementary DNA was reversed transcribed from viral RNA. Subgenomic cDNAs were obtained by direct cloning and polymerase chain reactions. Full length cDNA was constructed into the Bluescript II vector (pBSII) using unique, overlapping, restriction sites of four clones. The cDNA was infectious and produced echovirus 6 particles that behaved in the same manner as the parental virus.
Blackburn
R V
RV
Department of Immunology and Microbiology, Wayne State University, Detroit, MI 48201.
Racaniello
V R
VR
Righthand
V F
VF
D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004458:Echovirus 6, Human / Q000235:genetics* / Q000472:pathogenicity; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D004279:DNA, Viral