Evaluation of four methods for rapid detection of adenovirus.
Four methods for rapid detection of adenovirus were evaluated by testing retrospectively 28 frozen clinical specimens from which an adenovirus strain had been isolated. After thawing all specimens were retested for the presence of adenovirus by conventional culture on KB cells and found to be positive. The four tests used for rapid detection of adenovirus were a 48-hour culture technique, and an immunoassay, a latex agglutination test and an immunofluorescence assay for direct detection of viral antigen using commercially available reagents. Of the 28 specimens all were positive in the 48-hour culture, 25 (89%) positive in the immunoassay and 10 (36%) positive in the latex agglutination test. Six of eight nasopharyngeal aspirate specimens were positive in the immunofluorescence assay. Twenty-five clinical specimens negative for adenovirus on conventional culture were also negative in the 48-hour culture technique. Overall, the rapid (48-hour) culture technique was 100% sensitive and 100% specific compared to conventional culture. The direct detection of viral antigen by immunoassay was less sensitive, however results were available within a few hours. Prospective comparative studies are warranted to determine whether these rapid techniques could replace conventional culture in the routine diagnosis of adenovirus infection.
Trabelsi
A
A
Microbiology Laboratory, Faculty of Medicine, Jean Monnet University, St. Etienne, France.
Pozzetto
B
B
Mbida
A D
AD
Grattard
F
F
Ros
A
A
Gaudin
O G
OG
D000256:Adenoviridae / Q000276:immunology / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000032:analysis; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D012189:Retrospective Studies
D000956:Antigens, Viral