title: Rat ovarian apolipoprotein E: localization and gonadotropic control of messenger RNA.
abstract: Apolipoprotein E (apo E) is a 35-kDa protein found in association with various lipoproteins. It is synthesized by a wide variety of tissues, including the ovary. It can serve several functions, such as 1) transport of excess cholesterol from peripheral tissue to the liver; 2) directed movement of cholesterol from areas of high to low cholesterol concentration within tissue or organs; and 3) inhibition of the conversion of theca progesterone to androgen, thus acting as an autocrine or paracrine factor within the ovary. To better understand the physiological role of ovarian apo E, we employed the technique of in situ hybridization utilizing 35S-labeled apo E riboprobes to identify cells containing E mRNA. We studied ovaries of hypophysectomized immature rats administered various regimens of gonadotropins because of the uniform, predictable stimulation of follicular granulosa and theca development, ovulation, and corpus luteum formation. Apo E mRNA was localized predominantly in the theca, with an increase associated with theca hypertrophy. Apo E mRNA increased in granulosa cells with the development of preovulatory Graafian follicles, but decreased to baseline following ovulation and corpus luteum formation. These data are consistent with two roles for apo E in the ovary: 1) directing cholesterol to cells needing cholesterol as substrate for cell proliferation and steroidogenesis, and 2) acting as an autocrine regulatory factor to reduce theca androgen substrate for follicle estrogen production.
authors.affiliation: Department of Pathology, University of Chicago, Illinois 60637.
authors.forename: D
authors.initials: D
authors.lastname: Polacek
authors.forename: M W
authors.initials: MW
authors.lastname: Beckmann
authors.forename: J R
authors.initials: JR
authors.lastname: Schreiber
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics* / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D015342:DNA Probes; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D006080:Ovarian Follicle / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013799:Theca Cells / Q000378:metabolism
chemical_list: D001057:Apolipoproteins E; D006063:Chorionic Gonadotropin; D015342:DNA Probes; D012333:RNA, Messenger; D005640:Follicle Stimulating Hormone