Production of porcine parvovirus empty capsids with high immunogenic activity.
The VP2 gene of porcine parvovirus was cloned in the baculovirus system and expressed in insect cells. The resulting product was present in high yield. It self-assembled into particles which were structurally and antigenically indistinguishable from regular PPV capsids. A high degree of purity of the recombinant capsids was obtained by ammonium sulphate precipitation of cell lysates. These virus-like particles were used as antigen in the immunization of two pigs. The pigs elicited an immune response which, when assayed by standard serological techniques, was identical to that of a commercial vaccine. The amount of recombinant antigen needed in a vaccine dose was only 3 micrograms in a primary dose and 1.5 micrograms in the booster.
Martínez
C
C
Inmunologia y Genetica Aplicada SA (Ingenasa), Madrid, Spain.
Dalsgaard
K
K
López de Turiso
J A
JA
Cortés
E
E
Vela
C
C
Casal
J I
JI
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000276:immunology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors / Q000235:genetics; D006386:Hemagglutination Tests; D007114:Immunization; D008969:Molecular Sequence Data; D010321:Parvoviridae / Q000235:genetics*; D011994:Recombinant Proteins / Q000302:isolation & purification; D012641:Selection, Genetic; D013552:Swine; D014162:Transfection / Q000235:genetics
D004279:DNA, Viral; D011994:Recombinant Proteins