Peptide secondary structure induced by a micellar phospholipidic interface: proton NMR conformational study of a lipopeptide.
The conformational change of the model peptide Ac-K-G-R-G-D-G-amide induced by a phospholipidic interface was investigated by proton nuclear magnetic resonance (1H NMR). In aqueous solution, the free peptide is highly flexible and disordered, even in the presence of deuterated dodecyl-phosphocholine (DPC-d38) micelles which mimic a membrane interface. The lipopeptide, obtained by grafting a lipid anchor [2,3-dipalmitoyl-D-(+)-glyceric acid] to the lysine side chain of the peptide, was studied by standard 2D 1H NMR spectroscopy combined with distance geometry and simulated annealing calculations. When anchored to a micelle interface, the peptide acquires a definite turn (II/I') conformation. We were also able to describe precisely the conformation of the diacylglyceric fragment of the lipopeptide in a lipid environment and to establish the average orientation of the peptide segment with respect to the micelle surface.
Macquaire
F
F
Département de Biologie Cellulaire et Moléculaire, SBPM, URA CNRS 1290, Gif sur Yvette, France.
Baleux
F
F
Giaccobi
E
E
Huynh-Dinh
T
T
Neumann
J M
JM
Sanson
A
A
D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003903:Deuterium; D005988:Glyceric Acids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008074:Lipoproteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D010168:Palmitates / Q000737:chemistry*; D010743:Phospholipids; D010767:Phosphorylcholine / Q000031:analogs & derivatives; D011487:Protein Conformation; D012996:Solutions
D005988:Glyceric Acids; D008074:Lipoproteins; D008823:Micelles; D009842:Oligopeptides; D010168:Palmitates; D010743:Phospholipids; D012996:Solutions; D010767:Phosphorylcholine; C073582:2,3-dipalmitoylglyceric acid; C028810:dodecylphosphocholine; D003903:Deuterium