[Detection with nonradioactively labeled probes of the toxin genes of different E. coli pathotypes from swine].
We tested hemolytic E. coli from 86 pigs with edema disease or colidiarrhea. They were tested serologically and with nonradioactive digoxigenin-dUTP labelled probes for the presence of enterotoxin or Shiga-like-toxin genes. By slide-agglutination we detected 38 cases with E. coli O149:K88, 28 with E. coli O139:82B and 20 with E. coli O141. E. coli of serogroup O149:K88 isolated from diarrheic pigs, reacted with the probes for LT and STb genes. Edema disease E. coli O139:82B reacted with the SLTII probe. E. coli O141, isolated from colidiarrhea or edema disease showed a diversity of toxin gene patterns. All the E. coli O141 from diarrheic pigs reacted with the probes for LT and STap in addition to SLTII. No strains isolated from pigs with edema disease possessed any of these enterotoxin genes. Gene probe technique confirmed the serological method as useful tool for diagnosing E. coli O149:K88 and O139:82B as ETEC or VTEC, respectively. On the other hand only the demonstration of toxin genes with probes could explain the pathological findings in the pigs shedding E. coli of serogroup O141.
Boss
P
P
Institut für Veterinärbakteriologie, Universität Bern.
Monckton
R P
RP
Nicolet
J
J
Burnens
A P
AP
D000818:Animals; D015342:DNA Probes; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004488:Edema Disease of Swine / Q000382:microbiology*; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D015342:DNA Probes; D004768:Enterotoxins
Nachweis von Toxingenen verschiedener E. coli Pathotypen beim Schwein mit nichtradioaktiv markierten Sonden.