Vitamin K2 modulates proliferation and function of osteoblastic cells in vitro.
A human osteosarcoma cell line, HOS TE85 cells, and a mouse osteoblastic cell line, MC3T3-E1 cells, were cultured for 3 days in a medium containing various concentrations of menaquinone-4 (vitamin K2). As a result, the proliferation of HOS cells was suppressed by vitamin K2 in a dose dependent manner up to 56% of control by 10(-7)M of vitamin K2 and that of MC3T3-E1 cells was suppressed to 84% of control by 10(-6)M of vitamin K2. Vitamin K2 increased alkaline phosphatase activity in both kinds of cells. Warfarin counteracted the effect of vitamin K2 on osteoblastic cell proliferation. Our results show that vitamin K2 modulates proliferation and function of osteoblastic cells by some mechanisms including gamma-carboxylation system.
Akedo
Y
Y
Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
Hosoi
T
T
Inoue
S
S
Ikegami
A
A
Mizuno
Y
Y
Kaneki
M
M
Nakamura
T
T
Ouchi
Y
Y
Orimo
H
H
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D012516:Osteosarcoma; D014407:Tumor Cells, Cultured; D014812:Vitamin K / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D024482:Vitamin K 2 / Q000031:analogs & derivatives; D014859:Warfarin / Q000494:pharmacology
D024482:Vitamin K 2; D014812:Vitamin K; C030814:menatetrenone; D014859:Warfarin