Monoclonal antibodies affecting sperm-zona binding and/or zona-induced acrosome reaction.
The majority of anti-sperm monoclonal antibodies which were shown to inhibit in vitro fertilization in our laboratory react with antigens in the acrosomal region of spermatozoa. To elucidate the mechanism of human and/or mouse fertilization inhibition, the effects of these antibodies on mouse sperm-zona binding and mouse zona-induced acrosome reaction in vitro were studied. Among these antibodies, MS-4 and MS-7 were shown to react partially with uncapacitated mouse sperm, and to react more with capacitated mouse sperm, whereas HS-9, HS-11 and HS-63 react only with capacitated and acrosome-intact human or mouse sperm. HS-63 and MS-4 were shown to inhibit zona-induced acrosome reaction significantly, but not the sperm-zona binding. On the other hand, HS-9, HS-11 and MS-7 were shown to inhibit both sperm-zona binding and zona-induced acrosome reaction. The results of this study suggest that fertilization inhibition caused by these antibodies could result from that of the initial sperm-zona binding and/or zona-induced acrosome reaction.
Fann
C H
CH
Dept. Obstetrics and Gynecology, University of British Columbia, Vancouver, Canada.
Lee
C Y
CY
D000177:Acrosome / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005260:Female; D005307:Fertilization in Vitro; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013084:Sperm-Ovum Interactions / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology* / Q000502:physiology; D015044:Zona Pellucida / Q000502:physiology*
D000911:Antibodies, Monoclonal