title: Platelet responses to cardiopulmonary bypass. Assessment by a shear stress activation technique.
abstract: Platelet responses before and during cardiopulmonary bypass were studied using a shear stress activation technique. Whole blood was passed through a 10 microns micro-porous filter and the percentage of platelets retained was determined. Retention in control subjects (n = 20, age 62 +/- 11 years) was 65.2 +/- 15.1%. Patients with ischaemic heart disease (n = 20, age 61 +/- 9 years) had significantly higher retention at 82.6 +/- 13.2%, p less than 0.001. In valvular heart disease (n = 15, age 57 +/- 14 years) the retention rate was 43.5 +/- 15.4%, lower than both controls and ischaemic heart disease patients (p less than 0.001). In vitro haemodilution had no significant effect on platelet retention. High retention rates were maintained in patients undergoing coronary artery surgery. During valve surgery retention increased before aortic cross-clamp removal (83.5 +/- 13.4%) compared with baseline values (p less than 0.001) and remained high 24 hours postoperatively (79.6 +/- 9.4%). Platelet retention also increased within 20 minutes of thoracotomy without cardiopulmonary bypass (n = 10, age 57 +/- 8 years, 79.3 +/- 11.6% versus 61.6 +/- 10.9% baseline, p less than 0.01) and remained high 24 hours postoperatively. Significant differences in platelet responses, assessed by shear stress activation, were demonstrated between healthy controls and patients with ischaemic and valvular heart disease. Surgery, with or without extracorporeal circulation, caused a significant increase in platelet retention.
authors.affiliation: Department of Cardiothoracic Surgery, Oxford Heart Centre, John Radcliffe Hospital, England.
authors.forename: J
authors.initials: J
authors.lastname: Butler
authors.forename: G M
authors.initials: GM
authors.lastname: Rocker
authors.forename: J R
authors.initials: JR
authors.lastname: O'Brien
authors.forename: M
authors.initials: M
authors.lastname: Etherington
authors.forename: R
authors.initials: R
authors.lastname: Pillai
authors.forename: S
authors.initials: S
authors.lastname: Westaby
mesh_terms: D000368:Aged; D001792:Blood Platelets / Q000502:physiology*; D002315:Cardiopulmonary Bypass; D003327:Coronary Disease / Q000097:blood / Q000601:surgery; D005260:Female; D006349:Heart Valve Diseases / Q000097:blood / Q000601:surgery; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D013314:Stress, Mechanical; D013908:Thoracotomy