Effect of vehicle on skin penetration and retention of a lipophilic red tide toxin (PbTx-3).
The purpose of this study was to determine the effect of carrier vehicle on the penetration of brevetoxin ([3H]PbTx-3) into skin layers and receptor fluid. Disks of guinea-pig skin were mounted on penetration chambers. Epidermal surfaces were dosed with 0.320 microgram/cm2 of PbTx-3 dissolved in 50 microliters of vehicle [water, methanol, or dimethylsulfoxide (DMSO)]. In vitro skin penetration by PbTx-3 during 24 hr of exposure was 6.2, 2.3 and 26% for water, methanol, and DMSO, respectively (expressed as % of dose applied).
Kemppainen
B W
BW
Department of Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, AL 36849.
Mehta
M
M
Stafford
R
R
Riley
R T
RT
D000818:Animals; D006168:Guinea Pigs; D008297:Male; D008387:Marine Toxins / Q000008:administration & dosage / Q000493:pharmacokinetics*; D014677:Pharmaceutical Vehicles; D012867:Skin / Q000378:metabolism*
C017885:Gymnodinium breve toxin; D008387:Marine Toxins; D014677:Pharmaceutical Vehicles