Reassignment of the human macrophage colony stimulating factor gene to chromosome 1p13-21.
Macrophage colony stimulating factor (CSF-1) is a member of a family of glycoproteins that are necessary for the normal proliferation and differentiation of myeloid progenitor cells. The human CSF-1 gene has previously been assigned to chromosome 5 using somatic cell hybrids, and further localized to 5q33 by in situ hybridization with a 3H labelled cDNA probe. However, the murine macrophage colony stimulating factor gene (csfm) has been localized to a region on mouse chromosome 3 which was previously shown to be syntenic with the proximal region of 1p and not 5q. Using a human genomic DNA clone that contains the CSF-1 gene, we have localized CSF-1 to chromosome 1p13-21 by fluorescence in situ hybridization. The reassignment of the CSF-1 gene argues against its involvement in myeloid disorders with deletions of the long arm of chromosome 5.
Saltman
D L
DL
Department of Molecular Genetics, Genelabs Incorporated, Redwood City, CA 94063.
Dolganov
G M
GM
Hinton
L M
LM
Lovett
M
M
D000818:Animals; D016477:Artifacts; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007621:Karyotyping; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics*; D008677:Metaphase; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D013050:Spectrometry, Fluorescence
D009838:Oligodeoxyribonucleotides; D016173:Macrophage Colony-Stimulating Factor; D004247:DNA