[Design of a DNA-matrix with a specific structure for synthesizing RNA using the polymerase chain reaction].
Combination of chemical DNA synthesis and polymerase chain reactions has been used for obtaining DNA templates of preset structure coding for target mRNAs. The obtained DNA were 136 to 246 bp in length, contained promoters for RNA polymerases of phage T7 or E. coli and coded for 136-membered mRNA.
Smelkova
N V
NV
Elov
A A
AA
Shabarova
Z A
ZA
D000595:Amino Acid Sequence; D001345:Autoradiography; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012331:RNA, Fungal / Q000096:biosynthesis*; D013604:T-Phages / Q000201:enzymology; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004271:DNA, Fungal; D012331:RNA, Fungal; D012321:DNA-Directed RNA Polymerases
Konstruirovanie DNK-matrits zadannoĭ struktury dlia sinteza RNK posredstvom polimeraznykh tsepnykh reaktsiĭ.