Effect of p-chloromercuribenzoate on Clostridium perfringens beta toxin.
p-Chloromercuribenzoate (PCMB) was shown to bind to Clostridium perfringens beta toxin. Treatment of the toxin with N-ethylmaleimide (NEM), 5,5'-dithio-bis(2-nitro-benzoic acid) (DTNB), o-iodosobenzoate (OIBA) and metal ions such as Cu2+ and Ag+ decreased the lethal activity, but PCMB did not affect the lethal activity. On the other hand, the binding of PCMB to the toxin was inhibited by DTNB and NEM in a dose-dependent manner. Furthermore, the lethal activity of beta toxin pretreated with PCMB was not blocked by treatment with NEM, DTNB, OIBA, Cu2+ and Ag+. However, the PCMB-treated toxin treated with reduced glutathione, dithiothreitol, 2-mercaptoethanol, liver homogenate or serum from mice was inactivated by NEM.
Sakurai
J
J
Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
Fujii
Y
Y
Nagahama
M
M
D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism / Q000633:toxicity*; D002729:Chloromercuribenzoates / Q000378:metabolism / Q000494:pharmacology*; D003016:Clostridium perfringens; D003300:Copper / Q000494:pharmacology; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D008110:Liver Extracts / Q000494:pharmacology; D008297:Male; D051379:Mice; D012834:Silver / Q000494:pharmacology; D020245:p-Chloromercuribenzoic Acid
D001427:Bacterial Toxins; C031884:CPB protein, Clostridium perfringens; D002729:Chloromercuribenzoates; D008110:Liver Extracts; D012834:Silver; D020245:p-Chloromercuribenzoic Acid; D003300:Copper; D004228:Dithionitrobenzoic Acid; D005978:Glutathione; D005033:Ethylmaleimide