Ultrastructural observations on autolytic changes of atrial specific granules in pig heart.
The progressive autolytic changes of atrial specific granules (ASGS) up to 24 hours after death were studied electron microscopically. Tissue samples taken from the left auricular myocardium of four pig hearts at 0, 0.5, 1.5, 3.5, 6, 12 and 24 hours post mortem were fixed for 24 hours by immersion in 2.5 per cent cacodylate buffered glutaraldehyde (pH 7.4). Two pig hearts were kept at 20 degrees C and two at 4 degrees C. Although typical autolytic changes were seen in many organelles, no remarkable modifications in aspect, distribution, content and diameter of ASGS were observed in all samples. The results of this study indicate that ASGS are not morphologically affected by the autolytic processes, at least during the first 24 hours. Hence, the electron microscopic evaluation of ASGS does not need a particular fixation method such as perfusion. This may eliminate technical problems when slaughterhouse material is used.
Castagnaro
M
M
Dipartimento di Patologia Animale, Facoltà di Medicina Veterinaria, Università degli Studi, Turin, Italy.
Benatti
A
A
Guarda
F
F
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D001329:Autolysis; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D009206:Myocardium / Q000648:ultrastructure*; D011180:Postmortem Changes; D013552:Swine / Q000033:anatomy & histology*
D009320:Atrial Natriuretic Factor