title: A family of beta 7 integrins on human mucosal lymphocytes.
abstract: The heterodimeric protein complex recognized by the human mucosal lymphocyte 1 (HML-1) monoclonal antibody is expressed on 95% of intraepithelial lymphocytes but on only 1-2% of peripheral blood lymphocytes [Cerf-Bensusson, N., Jarry, A., Brousse, N., Lisowska-Grospierre, B., Guy-Grand, D. & Griscelli, C. (1987) Eur. J. Immunol. 17, 1279-1285]. We purified the smaller HML-1 subunit (105 kDa under nonreducing conditions) from hairy-cell leukemia cells and determined the N-terminal amino acid sequence of this chain. The 17 residues determined were identical to the deduced amino acid sequence encoded by an integrin beta 7 cDNA clone [Yuan, Q., Jiang, W.-M., Krissansen, G.W. & Watson, J.D. (1990) Int. Immunol. 2, 1097-1108]. Biochemical analysis of the larger HML-1 subunit (175 kDa under nonreducing conditions) suggested that it was a distinct member of the cleaved group of integrin alpha chains, which we designated alpha E. The beta 7 chain also was associated with the integrin alpha 4 subunit, suggesting that the HML-1 antigen (alpha E beta 7) and alpha 4 beta 7 constitute a beta 7 integrin family on mucosal lymphocytes. Interestingly, regulation of the expression of the HML-1 antigen was reciprocal to that of lymphocyte function-associated molecule 1 in the presence of transforming growth factor beta 1. We suggest that these beta 7 integrins may play a specific role in mucosal localization or adhesion and that the expression of the HML-1 antigen might be regulated by transforming growth factor beta 1 produced at or near epithelial tissues.
authors.affiliation: Laboratory of Immunochemistry, Dana-Farber Cancer Institute, Boston, MA.
authors.forename: C M
authors.initials: CM
authors.lastname: Parker
authors.forename: K L
authors.initials: KL
authors.lastname: Cepek
authors.forename: G J
authors.initials: GJ
authors.lastname: Russell
authors.forename: S K
authors.initials: SK
authors.lastname: Shaw
authors.forename: D N
authors.initials: DN
authors.lastname: Posnett
authors.forename: R
authors.initials: R
authors.lastname: Schwarting
authors.forename: M B
authors.initials: MB
authors.lastname: Brenner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D039641:Integrin beta Chains; D016023:Integrins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology*; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011498:Protein Precursors / Q000378:metabolism; D013601:T-Lymphocytes / Q000737:chemistry* / Q000166:cytology; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D039641:Integrin beta Chains; D016023:Integrins; D046911:Macromolecular Substances; D011498:Protein Precursors; D016212:Transforming Growth Factor beta; C070709:integrin alphaEbeta7; C091368:integrin beta7