title: A modified kanamycin-resistance cassette to facilitate two-codon insertion mutagenesis.
abstract: A kanamycin-resistance (KmR) cassette was modified to allow its introduction as a blunt-ended DNA fragment into target plasmids. Subsequent excision of the KmR cassette with ApaI and religation of the plasmid leaves behind six base pairs (5'-GGGCCC) that result in the insertion of two amino acids in the target protein.
authors.affiliation: Department of Microbiology and Immunology, SUNY-Health Science Center, Brooklyn 11203-2098.
authors.forename: W J
authors.initials: WJ
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Gross
authors.forename: K E
authors.initials: KE
authors.lastname: Joho
authors.forename: W T
authors.initials: WT
authors.lastname: McAllister
mesh_terms: D001483:Base Sequence; D003062:Codon; D004247:DNA; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D007613:Kanamycin Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D010957:Plasmids; D015183:Restriction Mapping; D013604:T-Phages / Q000235:genetics; D014764:Viral Proteins
chemical_list: D003062:Codon; D014764:Viral Proteins; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases