Common antigen lipopolysaccharide from Pseudomonas aeruginosa AK1401 as a receptor for bacteriophage A7.
A-band, a D-rhamnose-containing common lipopolysaccharide antigen isolated from Pseudomonas aeruginosa AK1401, was found to be a receptor for bacteriophage A7. The phage-borne rhamnanase was capable of hydrolyzing the A-band to expose core-lipid A containing only two or three rhamnose repeats. Interaction of the hydrolyzed A-band with core- or lipid A-specific monoclonal antibodies revealed that common epitopes exist in the inner core and lipid A regions, while the outer core of A-band appears to be different from that of the serotype-specific (B-band) lipopolysaccharide.
Rivera
M
M
Department of Biochemistry, Michigan State University, East Lansing 48824.
Chivers
T R
TR
Lam
J S
JS
McGroarty
E J
EJ
D000942:Antigens, Bacterial / Q000378:metabolism*; D001435:Bacteriophages / Q000201:enzymology / Q000378:metabolism*; D015153:Blotting, Western; D003837:Deoxy Sugars / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism*; D008351:Mannans / Q000378:metabolism; D008361:Mannosidases / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*
D000942:Antigens, Bacterial; D003837:Deoxy Sugars; D008070:Lipopolysaccharides; D008351:Mannans; D011135:Polysaccharides, Bacterial; D011991:Receptors, Virus; C065836:rhamnan; D008361:Mannosidases; C073767:rhamnanase