Thioredoxin gene expression is transcriptionally up-regulated by retinol in monkey conducting airway epithelial cells.
Using the differential hybridization technique, a cDNA clone, MT78, was isolated from the cDNA library of retinol-treated monkey tracheobronchial (TBE) epithelial cells. MT78 has a high sequence homology to human thioredoxin. The cDNA insert contains 506 nucleotides which encodes a peptide of 105 amino acids. The deduced peptide contains the highly conserved sequence Cys-Gly-Pro-Cys, found at the active site of all thioredoxins. The expression of the thioredoxin gene is stimulated 8-10 fold by vitamin A (retinol) in monkey TBE cells. The expression is significantly enhanced within 4 h after the vitamin A treatment and concurrent protein synthesis is not required for this enhancement. These results, in conjunction with the nuclear run-on transcriptional assay, support the conclusion that thioredoxin gene is transcriptionally up-regulated by retinol and/or its metabolites.
An
G
G
California Primate Research Center, University of California, Davis 95616.
Wu
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001980:Bronchi / Q000166:cytology / Q000187:drug effects*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013879:Thioredoxins / Q000187:drug effects / Q000235:genetics*; D014132:Trachea / Q000166:cytology / Q000187:drug effects*; D015854:Up-Regulation / Q000187:drug effects; D014801:Vitamin A / Q000494:pharmacology*
D014801:Vitamin A; D013879:Thioredoxins