title: Localization of HstI transcripts to the apical ectodermal ridge in the mouse embryo.
abstract: The HstI gene is a transforming gene, coding for a protein of the fibroblast growth factor family (Sakamoto et al., 1986). Previous RNA hybridization studies with the mouse homolog demonstrated the presence of a 3.0-kb transcript in Day 11 and 14 mouse embryos. Here we detect a 3.0-kb transcript in the limb and body of the dissected Day 11 mouse embryo. PCR amplification using HstI-specific primers also showed comparable results. In order to localize the HstI transcripts during development, corresponding HstI cDNA was isolated, and an HstI-specific region was used as a probe for in situ hybridization analysis. Serial sections of embryos from Day 8 (early-somite stages) through Days 9, 10, 11, and 12 of gestation were examined. With the antisense probe, a signal was detected in the Day 11 and 12 embryo, where it was localized to the apical ectodermal ridge (AER) of the limb bud. This structure is well known for its role in promoting the distal outgrowth of the developing limb bud. Signal was detected in both fore- and hindlimbs during the period of rapid distal growth. This restricted localization suggests a role for HstI in normal embryogenesis, including outgrowth of the limb bud.
authors.affiliation: Department of Biology, University of Iowa, Iowa City 52242.
authors.forename: H R
authors.initials: HR
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Sakamoto
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Sugimura
authors.forename: M
authors.initials: M
authors.lastname: Terada
authors.forename: M
authors.initials: M
authors.lastname: Solursh
mesh_terms: D000818:Animals; D015342:DNA Probes; D004475:Ectoderm / Q000378:metabolism*; D005121:Extremities / Q000196:embryology*; D051519:Fibroblast Growth Factor 4; D005346:Fibroblast Growth Factors / Q000235:genetics*; D006133:Growth Substances / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000196:embryology; D015513:Oncogene Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D015342:DNA Probes; C496446:Fgf4 protein, mouse; D051519:Fibroblast Growth Factor 4; D006133:Growth Substances; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D005346:Fibroblast Growth Factors