title: The effect of proanthocyanidin-rich hulls and proanthocyanidin extracts from bean (Vicia faba L.) hulls on nutrient digestibility and digestive enzyme activities in young chicks.
abstract: Proanthocyanidins were prepared from three bean (Vicia faba L.) varieties by extracting hulls in aqueous acetone. The amounts of freeze-dried extracts recovered were 74, 89 and 97 g/kg hull for the varieties Brunette, Statissa and Minica respectively. Chicks (3 weeks old) were fed on a maize-soya-bean control diet or the same control diet substituted with either 30 g proanthocyanidin extracts/kg or 300 g proanthocyanidin-rich hulls/kg. Chicks were tube-fed diets twice daily for 4 d. Nutrient digestibilities were calculated from amounts present in diets and freeze-dried excreta with the aid of titanium dioxide as a marker. Enzyme activities were measured in digesta removed from the jejunum. Extracts of proanthocyanidins depressed the digestibility of protein by 34%, starch by 3% and had no effect on the digestibility of lipid. Proanthocyanidin-rich hulls depressed the digestibility of protein by 62%, starch by 6% and lipid by 4%. Digestive enzyme activities were depressed to the same extent by extracts and hulls, trypsin (EC 3.4.21.4) by 55 and 62%, alpha-amylase (EC 3.2.1.1) by 75 and 78% and lipase (EC 3.1.1.3) by 31 and 32% for proanthocyanidin-extract and proanthocyanidin-rich-hull diets respectively. The susceptibility of substrates as well as enzymes to the effects of proanthocyanidins is discussed.
authors.affiliation: Instituto de Alimentacion Animal, Ciudad Universitaria, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Yuste
authors.forename: M
authors.initials: M
authors.lastname: Longstaff
authors.forename: C
authors.initials: C
authors.lastname: McCorquodale
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology*; D002645:Chickens; D004041:Dietary Fats / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism; D004063:Digestion / Q000187:drug effects*; D007887:Fabaceae / Q000737:chemistry / Q000378:metabolism*; D005766:Gastrointestinal Contents / Q000201:enzymology*; D008049:Lipase / Q000037:antagonists & inhibitors; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D044945:Proanthocyanidins; D013213:Starch / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000494:pharmacology; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D000872:Anthocyanins; D004041:Dietary Fats; D004044:Dietary Proteins; D010936:Plant Extracts; D044945:Proanthocyanidins; D014361:Trypsin Inhibitors; C013221:proanthocyanidin; D013213:Starch; D008049:Lipase; D000516:alpha-Amylases