Muscle phosphorus energy state in very-low-birth-weight infants: effect of exercise.
Skeletal muscle hypotonia is a hallmark clinical finding in very-low-birth-weight (VLBW) human infants. Although the biochemical basis for this phenomenon is not completely understood, one hypothesis is that the phosphorylation potential is abnormally low in the skeletal muscle of these infants. Therefore, we used 31P-nuclear magnetic resonance (NMR) spectroscopy to measure phosphorus metabolites in the skeletal muscle of VLBW infants during rest and during reflex-induced muscle contractions. Compared with healthy larger infants or to adults, the total phosphorus NMR signal is lower in VLBW infants. In VLBW infants during rest, [PCr]/([PCr]+[Pi]), where PCr is phosphocreatine and brackets denote concentration, was 89% and [ATP]/[ADP][Pi] was 59% of that found in larger infants (P less than 0.05). During reflex-induced isometric contractions in VLBW infants, [PCr]/([PCr]+[Pi]) declined by 24% and [ATP]/[ADP][Pi] declined by 35% (P less than 0.05 vs. rest). In all conditions, muscle pH remained 7.1. Overall, the differences in skeletal muscle energy state during rest and the corresponding changes in concentration of high-energy phosphates during mild exercise suggest a very limited energy reserve in the hypotonic muscle of VLBW infants.
Bertocci
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Rogers Magnetic Resonance Center, Department of Radiology, University of Texas Southwestern Medical Center, Dallas 75235-9085.
Mize
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Uauy
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D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D004734:Energy Metabolism; D006801:Humans; D007230:Infant, Low Birth Weight / Q000378:metabolism*; D007231:Infant, Newborn; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D009119:Muscle Contraction; D009132:Muscles / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D005082:Physical Exertion; D012016:Reference Values
D010710:Phosphates; D010725:Phosphocreatine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate