title: Phenobarbital and sulfonylurea-inducible operons encoding herbicide metabolizing cytochromes P-450 in Streptomyces griseolus.
abstract: We have identified the promoters for two inducible genes, in Streptomyces griseolus, that encode herbicide-metabolizing cytochromes P-450. They are in the class of promoters that have -35 and -10 sequences similar to those used in Escherichia coli by RNA polymerase E sigma 70. Transcription from either promoter was shown to be induced by sulfonylurea (chlorimuron ethyl) or phenobarbital. Mapping of mRNA showed that each cytochrome P450-encoding gene was transcribed on a separate multicistronic mRNA that encodes cytochrome P-450 (suaC or subC), ferredoxin (suaB or subB) and at least one other open reading frame. An inducible, site-specific DNA-binding activity was identified that bound to two similar 8-bp inverted repeat sequences within or near the sua promoter (suaP). A noninducible DNA-binding activity, distinct from that which bound to suaP, was found that bound to an 11-bp inverted repeat at the sub transcription start point.
authors.affiliation: E.I. DuPont and Co., Central Research and Development Department, Experimental Station, DE 19880-0173.
authors.forename: N V
authors.initials: NV
authors.lastname: Patel
authors.forename: C A
authors.initials: CA
authors.lastname: Omer
mesh_terms: D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005288:Ferredoxins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D006540:Herbicides / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D010634:Phenobarbital / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D013453:Sulfonylurea Compounds / Q000494:pharmacology*
chemical_list: D004268:DNA-Binding Proteins; D005288:Ferredoxins; D006540:Herbicides; D013453:Sulfonylurea Compounds; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital