Comparative studies of galactose-6-mustard and L-phenylalanine mustard on cell growth and cell cycle kinetics in vitro.
The effect of galactose-6-mustard (G-6-M) on cell growth and cell cycle kinetics was studied in murine P388 leukemia and Chinese hamster ovary (CHO) cells in vitro and compared with the effect of L-phenylalanine mustard (L-PAM). The IC50 values of G-6-M for the P388 and CHO cells were 10 and 100 microM, respectively. No difference of the IC50 value of L-PAM (2 microM) between the two cell lines was found. The effect of G-6-M and L-PAM on cell kinetics was similar for the two cell lines at IC50 doses. The relative cell outflow from the G2 stage was inhibited to a higher extent than the relative cell outflow from the S phase. The relative cell outflow from the G1 stage was only partly inhibited. These results are discussed in relation to growth conditions, differences in DNA repair capacity, and cellular uptake of G-6-M between P388 and CHO cells.
Stenberg
K
K
Department of Medical Radiobiology, Karolinska Institute, Stockholm, Sweden.
Skog
S
S
Tribukait
B
B
Borg
A L
AL
D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D016466:CHO Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005688:Galactosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007941:Leukemia P388 / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008558:Melphalan / Q000494:pharmacology*; D051379:Mice; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000477:Alkylating Agents; D004273:DNA, Neoplasm; D014316:Tritium; C050475:C6-galactose mustard; D005688:Galactosamine; D008558:Melphalan; D013936:Thymidine