A novel format for a rapid sandwich EIA and its application to the identification of snake venoms.
A rapid sandwich enzyme immunoassay format is described where conjugate is lyophilised within the well in which the test reaction will occur. The format is straight forward to manufacture, has a prolonged shelf life, and eliminates one incubation and wash step from the usual test procedure. The technology has been applied to the development of a rapid assay for the identification of snake venom in clinical specimens. The resultant assay was specific and sensitive, provided rapid results and was appropriate for field use.
Cox
J C
JC
Immunodiagnostics Section, Commonwealth Serum Laboratories Limited, Parkville, Victoria, Australia.
Moisidis
A V
AV
Shepherd
J M
JM
Drane
D P
DP
Jones
S L
SL
D007124:Immunoenzyme Techniques; D011933:Reagent Kits, Diagnostic; D012910:Snake Venoms / Q000032:analysis / Q000276:immunology*
D011933:Reagent Kits, Diagnostic; D012910:Snake Venoms