Mapping the entry of reactive oxygen metabolites into target erythrocytes during neutrophil-mediated antibody-dependent cellular cytotoxicity.
Transmitted Soret band optical microscopy has been used to image the entry and passage of reactive oxygen metabolites across target erythrocytes. Due to the rapid cytosolic diffusion of hemoglobin in comparison to video rates, it was necessary to use erythrocytes with relatively immobilized hemoglobin. To achieve this, erythrocytes from patients with sickle cell anemia were used. The movement of reactive oxygen metabolites across rabbit IgG-opsonized sickle cells was observed in real time. These observations indicate that reactive oxygen metabolites can enter and cross targets in an asymmetric fashion.
Petty
H R
HR
Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202.
Liang
B
B
Maher
R J
RJ
D000755:Anemia, Sickle Cell / Q000097:blood; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D003593:Cytoplasm / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D009504:Neutrophils / Q000276:immunology*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D012756:Sheep
D010100:Oxygen