title: Cell-to-cell contact between normal fibroblasts and lymphoblasts deficient in lysosomal enzymes.
abstract: Human lymphoblasts deficient in iduronate sulfatase or in alpha-N-acetylglucosaminidase acquire discrete levels of enzyme activity after co-culture with human normal skin fibroblasts. This occurs by direct cell-to-cell contact and not by uptake of secreted fibroblast enzyme. The process is dependent on time and on the number of fibroblasts used. Electron-microscopic examination of the co-culture of the two cell types reveals extensive region of intimate contact. Fibroblastic projections appear frequently in close apposition with lymphoblast invaginations; a diffuse micropinocytotic activity is evident only in fibroblastic cells.
authors.affiliation: Dipartimento di Biochimica e Biotecnologie Mediche, II Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli Federico II, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Di Natale
authors.forename: T
authors.initials: T
authors.lastname: Annella
authors.forename: A
authors.initials: A
authors.lastname: Daniele
authors.forename: R
authors.initials: R
authors.lastname: Negri
authors.forename: L
authors.initials: L
authors.lastname: Nitsch
mesh_terms: D000118:Acetylglucosaminidase / Q000172:deficiency / Q000378:metabolism*; D002450:Cell Communication; D002472:Cell Transformation, Viral; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology; D006801:Humans; D007066:Iduronate Sulfatase / Q000378:metabolism*; D008214:Lymphocytes / Q000166:cytology* / Q000201:enzymology; D008854:Microscopy, Electron; D009083:Mucopolysaccharidoses / Q000201:enzymology; D016532:Mucopolysaccharidosis II
chemical_list: D007066:Iduronate Sulfatase; D000118:Acetylglucosaminidase