[Immobilization of EcoR1, Pae1 and Lpl1 restriction enzymes].
In search for sorbents (silica gels, styrene-divinylbenzene copolymers), for immobilization of some restriction endonucleases, derivatives of trityl-containing silochroms are shown to bind EcoRI, PaeI and LplI endonucleases with the retention of 10-20, 60-70 and 40-60% activity, respectively. The immobilized restriction endonucleases have the unchanged substrate specificity, can be used several times and are stable at storage. Tritylaminopropylsilochrom is suggested to be the sorbent of choice.
Kovalenko
N A
NA
Sokolov
N N
NN
Cherkasova
T A
TA
Vonskiĭ
V E
VE
Leĭkin
Iu A
IuA
D010582:Bacteriophage lambda / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004800:Enzymes, Immobilized
D004279:DNA, Viral; D004800:Enzymes, Immobilized; D015246:Deoxyribonuclease EcoRI; C046184:endodeoxyribonuclease PaeI; C032824:ATCGAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Immobilizatsiia restriktaz EcoR1, Pae1 i Lpl1]