Molecular and genetic analysis of the yeast early meiotic recombination genes REC102 and REC107/MER2.
By selecting for mutations which could rescue the meiotic lethality of a rad52 spo13 strain, we isolated several new Rec genes required relatively early in the meiotic recombination process. This paper presents data to confirm that two of them, REC102 and REC107, are general, meiosis-specific recombination genes that have no detectable role during mitosis. Sequence analysis and genetic complementation indicate that REC107 is identical to the MER2 gene. No sequences related to REC102 have been found in the GenBank or EMBL collections. REC102 is expressed only in meiosis, prior to the reductional division, at about the time that genetic recombination occurs. Examination of the REC102 sequence indicates the presence of several sequences which may play a role in the regulation of its expression; however, the URS1 sequence commonly found in genes expressed early in meiosis is not present.
Cool
M
M
Department of Biology, University of Iowa, Iowa City 52242.
Malone
R E
RE
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004260:DNA Repair; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D004171:Diploidy; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007700:Kinetics; D008540:Meiosis / Q000235:genetics*; D008969:Molecular Sequence Data; D045522:Recombinases; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C073372:REC102 protein, S cerevisiae; C071054:REC107 protein, S cerevisiae; D045522:Recombinases; D029701:Saccharomyces cerevisiae Proteins