Glucose regulates the expression of Gi-proteins in cultured BC3H-1 myocytes.
To examine whether glucose has regulatory effects on the expression of Gi-proteins, BC3H-1 myocytes were incubated for 24 hr in the presence of various concentrations of glucose (0-25 mM) and the amount of Gi-proteins was detected by pertussis toxin ADP-ribosylation and immunoblot analysis. Both detection methods showed a progressive decrease in the amount of Gi proteins in cells treated with increasing concentrations of glucose. A maximal reduction of 40% was observed after a 24 hr exposure to 25 mM glucose. The reduction in Gi-proteins correlated with a decrease in insulin-stimulated glucose transport.
Moises
R S
RS
Department of Medicine, University of California, San Diego, La Jolla 92093.
Heidenreich
K A
KA
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003847:Deoxyglucose / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D007106:Immune Sera; D015151:Immunoblotting; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000378:metabolism
D007106:Immune Sera; D009842:Oligopeptides; D010566:Virulence Factors, Bordetella; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D003847:Deoxyglucose; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D005947:Glucose