Occurrence in vivo of 5-hydroxytryptophol in the brain of rats treated with ethanol.
The effect of ethanol (EtOH) on the release of dopamine (DA) and 5-hydroxytryptamine (5-HT) and the efflux of their metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxyindol-3-ylacetic acid (5-HIAA) and 5-hydroxytryptophol (5-HTOL) from the striatum of the freely moving rat were studied in vivo using brain microdialysis. Striatal DA and 5-HT release was maximally enhanced at first fraction after the administration of EtOH (2 g/kg, i.p.). The level of the DA-oxidized metabolite, DOPAC, decreased significantly. In the 5-HT metabolic pathway, the oxidized metabolite, 5-HIAA, did not show significant changes, whereas levels of the biogenic alcohol 5-HTOL were increased to 180% at 90 min following EtOH administration. It is suggested that EtOH, most probably via acetaldehyde, could shift 5-HT metabolism from the oxidative to the reductive pathway in the rat brain.
Yoshimoto
K
K
Department of Legal Medicine, Kyoto Prefectural University of Medicine, Japan.
Komura
S
S
Kawamura
K
K
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000096:biosynthesis; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D000431:Ethanol / Q000008:administration & dosage*; D006917:Hydroxytryptophol / Q000378:metabolism*; D008297:Male; D051381:Rats; D012701:Serotonin / Q000378:metabolism
D015102:3,4-Dihydroxyphenylacetic Acid; D006917:Hydroxytryptophol; D012701:Serotonin; D000431:Ethanol; D000444:Aldehyde Dehydrogenase; D004298:Dopamine