[Cloning and expression of the gene for Escherichia coli thermostable enterotoxin].
Gene estA coding for thermostable enterotoxin of Escherichia coli has been cloned. It is shown that in the E. coli strain SA162 this gene is located on the chromosome. Using polymerase chain reaction a site-directed mutagenesis of the cloned gene has been carried out, resulted in a recombinant strain--producer of the thermostable enterotoxin.
Mikul'skis
A V
AV
Domoradskaia
T I
TI
Filippov
S A
SA
Dobrynin
V N
VN
Korobko
V G
VG
D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D015139:Blotting, Southern; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D015870:Gene Expression; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D016133:Polymerase Chain Reaction
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli
Klonirovanie i ékspressiia gena termostabil'nogo énterotoksina Escherichia coli.