Sucrose fermentation by Fusobacterium mortiferum ATCC 25557: transport, catabolism, and products.
Studies of sucrose utilization by Fusobacterium mortiferum ATCC 25557 have provided the first definitive evidence for phosphoenolpyruvate-dependent sugar:phosphotransferase activity in the family Bacteroidaceae. The phosphoenolpyruvate-dependent sucrose:phosphotransferase system and the two enzymes required for the dissimilation of sucrose 6-phosphate are induced specifically by growth of F. mortiferum on the disaccharide. Monomeric sucrose 6-phosphate hydrolase (M(r), 52,000) and a dimeric ATP-dependent fructokinase (subunit M(r), 32,000) have been purified to electrophoretic homogeneity. The physicochemical and catalytic properties of these enzymes have been examined, and the N-terminal amino acid sequences for both proteins are reported. The characteristics of sucrose 6-phosphate hydrolase and fructokinase from F. mortiferum are compared with the same enzymes from both gram-positive and gram-negative species. Butyric, acetic, and D-lactic acids are the end products of sucrose fermentation by F. mortiferum. A pathway is proposed for the translocation, phosphorylation, and metabolism of sucrose by this anaerobic pathogen.
Thompson
J
J
Laboratory of Microbial Ecology, National Institute of Dental Research, Bethesda, Maryland.
Nguyen
N Y
NY
Robrish
S A
SA
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000595:Amino Acid Sequence; D001693:Biological Transport, Active; D002087:Butyrates / Q000378:metabolism; D004790:Enzyme Induction; D005673:Fusobacterium / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008969:Molecular Sequence Data; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D013395:Sucrose / Q000378:metabolism*; D043324:beta-Fructofuranosidase
D000085:Acetates; D002087:Butyrates; D007773:Lactates; D019344:Lactic Acid; D013395:Sucrose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D010732:Phosphofructokinase-1; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D019342:Acetic Acid