title: Adenosine stimulates breathing in fetal sheep with brain stem section.
abstract: Breathing responses to adenosine were determined in 12 chronically catheterized fetal sheep (greater than 0.8 term) in which hypoxic inhibition of breathing had been eliminated by brain stem section. The caudal extent of transection varied from the rostral midbrain to the pontomedullary junction. Isocapnic hypoxia [delta arterial PO2 (PaO2) of -12 Torr] doubled the incidence and depth of breathing activity and increased the incidence of eye movements. Intra-arterial infusion of adenosine (0.30 +/- 0.03 mg.min-1.kg fetal wt-1) increased the incidence and amplitude of breathing without affecting blood gases. Adenosine did not significantly alter the incidence of eye activity. Intra-arterial injection of oligomycin (120 +/- 26 micrograms/kg fetal wt), an inhibitor of mitochondrial oxidative phosphorylation, also stimulated breathing activity. In four fetuses with brain stem section, peripheral arterial chemodenervation blunted the stimulatory effects of hypoxia on breathing activity and abolished altogether the excitatory effects of adenosine. It is concluded that 1) hypoxia and adenosine likely inhibit breathing in normal fetuses by affecting similar areas of the brain stem and 2) in fetuses with brain section, hypoxic hyperpnea depends on peripheral and central mechanisms, whereas adenosine stimulates breathing via the peripheral arterial chemoreceptors.
authors.affiliation: Department of Obstetrics and Gynecology, Nicholas S. Assali Perinatal Research Laboratory, University of California, Los Angeles School of Medicine 90025.
authors.forename: B J
authors.initials: BJ
authors.lastname: Koos
authors.forename: A
authors.initials: A
authors.lastname: Chao
authors.forename: W
authors.initials: W
authors.lastname: Doany
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D001933:Brain Stem / Q000502:physiology*; D002628:Chemoreceptor Cells / Q000503:physiopathology; D003714:Denervation; D005260:Female; D005311:Fetal Hypoxia / Q000503:physiopathology; D005333:Fetus / Q000187:drug effects* / Q000502:physiology; D009840:Oligomycins / Q000494:pharmacology; D011247:Pregnancy; D012119:Respiration / Q000187:drug effects* / Q000502:physiology; D012756:Sheep
chemical_list: D009840:Oligomycins; D000241:Adenosine