title: Effects of halothane and other volatile anaesthetics on protein synthesis and secretion in guinea pig liver slices.
abstract: We have investigated the effect of volatile anaesthetics on protein synthesis and secretion in Hartley male guinea pig liver slices. The slices (250-300 microns thick) were incubated in sealed roller vials containing Krebs-Henseleit buffer at 37 degrees C under 95% oxygen. Volatile anaesthetics were vaporized in the vials to produce constant concentrations in the medium. Halothane 1-2.1 mmol litre-1 produced a concentration-related decrease in protein synthesis (3H-leucine incorporation) and secretion. Deuterated halothane (d-halothane), which is less biotransformed, was less inhibiting than halothane: uptake of the 3H-leucine was not affected but its incorporation into the nascent peptide was inhibited. Enflurane 2.2 mmol litre-1, isoflurane 2.2 mmol litre-1 and sevoflurane 2.1 mmol litre-1 also inhibited protein synthesis, but to a lesser extent than halothane and d-halothane. We conclude that alterations in protein synthesis and secretion are an early and sensitive indicator of cellular injury by volatile anaesthetics in liver slices.
authors.affiliation: Department of Anesthesiology, University of Arizona, Tucson 85724.
authors.forename: H N
authors.initials: HN
authors.lastname: Ghantous
authors.forename: J L
authors.initials: JL
authors.lastname: Fernando
authors.forename: R L
authors.initials: RL
authors.lastname: Keith
authors.forename: A J
authors.initials: AJ
authors.lastname: Gandolfi
authors.forename: K
authors.initials: K
authors.lastname: Brendel
mesh_terms: D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D003864:Depression, Chemical; D006168:Guinea Pigs; D006221:Halothane / Q000494:pharmacology*; D066298:In Vitro Techniques; D007930:Leucine / Q000493:pharmacokinetics; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D013997:Time Factors
chemical_list: D000777:Anesthetics; D011506:Proteins; D007930:Leucine; D006221:Halothane