Anti-epidermal growth factor antibody inhibits compensatory renal hyperplasia but not hypertrophy after unilateral nephrectomy in mice.
The effect of anti-epidermal growth factor (EGF) antibody on the compensatory renal growth including hyperplasia and hypertrophy was investigated. The antibody used in this study blocked the stimulatory effect of EGF on the DNA synthesis of cultured rat hepatocytes. When this antibody was injected into mice intravenously after unilateral nephrectomy, the labeling index of the renal cortical tubular cells decreased significantly at the second day after uninephrectomy, but the antibody caused no decrease in remaining kidney weight. Immunohistochemical study revealed that injected anti-mouse EGF rabbit IgG was positively stained at the renal cortical tubular cells. EGF would thus appear importantly essential to compensatory renal hyperplasia.
Kanda
S
S
Department of Urology, Nagasaki University School of Medicine, Japan.
Igawa
T
T
Sakai
H
H
Nomata
K
K
Kanetake
H
H
Saito
Y
Y
D000818:Animals; D000906:Antibodies; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000276:immunology / Q000502:physiology*; D006965:Hyperplasia; D006984:Hypertrophy; D007116:Immunization, Passive; D007150:Immunohistochemistry; D007668:Kidney / Q000473:pathology*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D009392:Nephrectomy; D009929:Organ Size; D051381:Rats
D000906:Antibodies; D004815:Epidermal Growth Factor; D004247:DNA