Salivary anti-candidal assays.
Inhibition of Candida albicans blastospore viability by parotid, submandibular-sublingual and whole salivas could not be determined by direct assay of yeast cells in each respective saliva. Determination of antifungal activity could, however, be carried out if saliva was first preincubated with Candida cells and this was immediately followed by removal of saliva and resuspension of yeast cells in nonenriched buffers of pH 5-7 for appropriate incubation periods. To attain accurate reproducible quantitative data, parotid, submandibular-sublingual and whole salivas each required different preincubation times with C. albicans as well as prior acidification and boiling. Acidification was also necessary for optimizing the germ tube assay although, in contrast to blastospore viability, inhibition of blastospore-germ tube conversion could be determined directly in saliva. Salivary antifungal effects on blastospore division were negligible at yeast cell concentrations greater than 10(6) colony-forming units per ml and were found to be independent of pH, whereas salivary inhibition of germ tube formation was significant only at pH 5 in the assay systems employed. The requirement for acidification and an observed enhancement of antifungal activity on aqueous dilution of the saliva suggested that only a fraction of the salivary antifungal components present in saliva were available in the free form to exert their biological activity. These results open up the possibility of investigating salivary antifungal activity in human health and disease.
Santarpia
R P
RP
School of Dental Medicine, State University of New York, Stony Brook.
Xu
L
L
Lal
K
K
Pollock
J J
JJ
D000935:Antifungal Agents; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development*; D015169:Colony Count, Microbial; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012463:Saliva / Q000382:microbiology* / Q000502:physiology; D012471:Salivary Proteins and Peptides / Q000502:physiology*; D013172:Spores, Fungal / Q000187:drug effects
D000935:Antifungal Agents; D012471:Salivary Proteins and Peptides