title: [Interphase cytogenetic studies of human X chromosome].
abstract: The chromosome in situ hybridization with human X chromosome alpha satellite DNA probe (pBamX7) on human lymphocyte metaphases and interphase nuclei was performed for interphase cytogenetic studies. The individuals with numerical or structural abnormalities of X chromosome were studied. The results showed that the probe hybridized specifically to the centromeric region (p11----q11) of X chromosome. The number of silver grain clusters in interphase nuclei was correlated with that of X chromosome. Most of the clusters located near the nuclear membrane where inactive X chromatins (Barr-bodies) were usually found. The method of ascertaining the number of X chromosomes by in situ hybridization was much more reliable than that by counting the number of Barr-bodies. The modified R-banding technique was introduced and the significance of this work was also discussed.
authors.affiliation: Institute of Basic Medical Sciences, CAMS, Beijing.
authors.forename: H
authors.initials: H
authors.lastname: Qiu
authors.forename: Z
authors.initials: Z
authors.lastname: Cheng
authors.forename: C
authors.initials: C
authors.lastname: Gao
authors.forename: L
authors.initials: L
authors.lastname: Ye
authors.forename: S
authors.initials: S
authors.lastname: Ma
authors.forename: J
authors.initials: J
authors.lastname: Zhu
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Fu
mesh_terms: D002869:Chromosome Aberrations; D002871:Chromosome Banding; D005260:Female; D006801:Humans; D007399:Interphase; D008297:Male; D009693:Nucleic Acid Hybridization; D014960:X Chromosome / Q000648:ultrastructure*