Two signals are involved in polyclonal B cell stimulation by T helper type 2 cells: a role for LFA-1 molecules and interleukin 4.
T helper cell type 2 (Th2) cells when triggered by antibodies to CD3 acquire the capacity to stimulate the polyclonal proliferation of syngeneic, resting B cells. Here, we tested the ability of various monoclonal antibodies (mAb) to block the B cell proliferation-inducing potential of such activated Th2 cells. We demonstrate that anti-interleukin 4, as well as anti-LFA-1 antibodies interfere with the T-B cell interaction. In kinetic studies, anti-LFA-1 was found to be operative during the first half and anti-interleukin 4 during the second half of the 48-h culture period. This defines at least two different steps in B cell triggering by Th2 cells. In addition, the data imply that the T-B cell interaction involves an additional structure, namely an activation molecule, on the T cell surface.
Lohoff
M
M
Institut für Klinische Mikrobiologie, Universität Erlangen-Nürnberg, FRG.
Koch
A
A
Röllinghoff
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D001402:B-Lymphocytes / Q000276:immunology*; D017252:CD3 Complex; D002478:Cells, Cultured; D005260:Female; D066298:In Vitro Techniques; D015847:Interleukin-4 / Q000502:physiology*; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D016169:Lymphocyte Function-Associated Antigen-1; D011948:Receptors, Antigen, T-Cell; D015847:Interleukin-4