The nature of an accessory cell in bone marrow stimulating murine megakaryocytopoiesis.
The nature of accessory cells in unstimulated mouse bone marrow that modulates in vitro megakaryocytopoiesis by releasing megakaryocyte potentiator (Mk-potentiator) activity was investigated. An active cell class was identified by the 7/4 and Ia cellular antigens. Bone marrow macrophages were obtained by their defined outgrowth in the presence of growth factors. Bone marrow macrophages grown in the presence of interleukin 3 (IL-3) were more active in producing Mk-potentiator than were macrophages grown in the presence of colony-stimulating factor 1 (CSF-1). The data indicate that the accessory cells producing Mk-potentiator are a select population of bone marrow macrophages that may be responsible for inducing megakaryocyte maturation under steady-state conditions.
Jackson
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Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.
Williams
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N
Banu
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D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000502:physiology*; D001853:Bone Marrow / Q000378:metabolism / Q000502:physiology; D001854:Bone Marrow Cells; D002455:Cell Division / Q000502:physiology; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D058851:GPI-Linked Proteins; D006410:Hematopoiesis / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007377:Interleukin-3 / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008533:Megakaryocytes / Q000166:cytology* / Q000502:physiology; D008562:Membrane Glycoproteins; D000090204:Mesothelin; D051379:Mice; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000378:metabolism / Q000502:physiology
D003115:Colony-Stimulating Factors; D058851:GPI-Linked Proteins; D000949:Histocompatibility Antigens Class II; D007377:Interleukin-3; D008562:Membrane Glycoproteins; D011506:Proteins; D000090204:Mesothelin