A simple method of generating axenic derivatives of Dictyostelium strains.
We describe a generally applicable method of adapting Dictyostelium from growth on a bacterial food source to axenic growth. Cells are initially selected by growth on a plastic substratum but subsequently acquire the ability to grow in suspension culture. These strains can be transformed efficiently by DNA-mediated gene transfer.
Morrison
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Imperial Cancer Research Fund, Clare Hall Laboratory, South Mimms, Herts, United Kingdom.
Harwood
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D000818:Animals; D004023:Dictyostelium / Q000254:growth & development*; D005856:Germ-Free Life; D010641:Phenotype; D010957:Plasmids