The effect of butylated hydroxytoluene, with and without cortisol, on stimulated lymphocytes.
In the present work we undertook to ascertain whether butylated hydroxytoluene (BHT), which is used in food as an antioxidant, is capable of either inhibiting human lymphocyte stimulation or acting synergistically with cortisol and prednisolone to the same end. BHT cytotoxicity was observed at concentrations higher than 100 micrograms/ml. In the concentration range of 0.0 to 60.0 micrograms/mL, BHT showed no effect on the uptake of 3H-thymidine by PHA stimulated lymphocytes. However, at 50 micrograms/mL BHT suppressed mixed lymphocyte reaction (MLR). A synergistic effect with regard to suppression of PHA stimulated lymphocytes was observed when the cells were incubated with BHT in the presence of either cortisol or prednisolone.
Klein
A
A
Department of Medical Laboratories, Bar-Ilan University, Kfar Saba, Israel.
Bruser
B
B
D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D007958:Leukocyte Count / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000187:drug effects; D011239:Prednisolone / Q000494:pharmacology
D002084:Butylated Hydroxytoluene; D011239:Prednisolone; D006854:Hydrocortisone