Oncostatin M binds the high-affinity leukemia inhibitory factor receptor.
Oncostatin M (OSM) is a glycoprotein cytokine that was recently demonstrated to be structurally and functionally related to the leukemia inhibitory factor (LIF). We have investigated the binding of each cytokine to a variety of cellular receptors including those on solid tumor lines, leukemic cells, endothelial cells, macrophages, and cells transfected with the recently cloned low-affinity LIF receptor, and to a soluble form of the LIF receptor. LIF is incapable of binding either high- or low-affinity OSM receptors, yet OSM is capable of binding the high-affinity but not the low-affinity LIF receptor. Since the presence of high-affinity LIF receptors correlates with the biological activity of LIF on a wide range of target cells, we predict that OSM should have similar effects on LIF-responsive cells.
Gearing
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DP
Immunex Research and Development Corporation, Seattle, WA 98101.
Bruce
A G
AG
D000818:Animals; D003001:Cloning, Molecular; D016207:Cytokines / Q000378:metabolism*; D006131:Growth Inhibitors; D006801:Humans; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines; D053683:Oncostatin M; D010455:Peptides / Q000378:metabolism*; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D053606:Receptors, OSM-LIF; D014407:Tumor Cells, Cultured
D016207:Cytokines; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; C507840:LIFR protein, human; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines; C508351:OSM protein, human; D010455:Peptides; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic; D053606:Receptors, OSM-LIF; D053683:Oncostatin M