A novel mutation FruS, altering synthesis of components of the phosphoenolpyruvate: fructose phosphotransferase system in Escherichia coli K12.
A novel mutation, FruS localised in the fru operon was obtained. It uncouples expression of the genes determining synthesis of the fructose-specific transport proteins and fructose-1-phosphate kinase. In FruS bacteria the fruA and fruF genes (coding for Enzyme IIfru and FPr, respectively) are constitutive by expressed while fruK (encoding fructose-1-phosphate kinase) remains inducible. In contrast to other mutations, which render expression of the whole fru operon constitutive, the FruS mutation: (1) does not lead to D-xylitol sensitivity; (2) does not inhibit growth on D-lactate, pyruvate and L-alanine; (3) does not decrease phosphoenolpyruvate (PEP) synthase activity.
Bolshakova
T N
TN
N.F. Gamaleya Institute for Epidemiology and Microbiology, Academy of Medical Sciences, Moscow, USSR.
Molchanova
M L
ML
Erlagaeva
R S
RS
Grigorenko
Y A
YA
Gershanovitch
V N
VN
D000409:Alanine / Q000378:metabolism; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D005838:Genotype; D007773:Lactates / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D010732:Phosphofructokinase-1 / Q000235:genetics / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D014161:Transduction, Genetic; D014993:Xylitol / Q000378:metabolism
D007773:Lactates; D011773:Pyruvates; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D010732:Phosphofructokinase-1; C068652:1-phosphofructokinase; D000409:Alanine; D008358:Mannose; D014993:Xylitol