Immobilization of immunoglobulin on a quartz surface by BrCN without loss of antigen binding capability.
BrCN-activated quartz was used for the immobilization of monoclonal immunoglobulins. BrCN-activated quartz keeps its ability to quantitatively bind immunoglobulins for at least 8 h. The surface density of the immobilized immunoglobulins was 100 ng cm-2 and they were found to retain their ability to bind antigen in a molar ratio of 1:2 for not less than three months.
Krapivinskaya
L D
LD
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow.
Krapivinsky
G B
GB
Ratner
V L
VL
D000937:Antigen-Antibody Reactions / Q000276:immunology*; D015374:Biosensing Techniques; D003488:Cyanogen Bromide; D007118:Immunoassay; D007136:Immunoglobulins / Q000737:chemistry*; D011791:Quartz; D013499:Surface Properties
D007136:Immunoglobulins; D011791:Quartz; D003488:Cyanogen Bromide