Isolation of the rhabdomeral microvillar cytoskeleton of the crayfish (Orconectes limosus) photoreceptor by a crosslinking reagent.
1. Rhabdomeral microvilli of photoreceptor cells of invertebrates contain a labile central cytoskeleton. For stabilization of the rhabdomeral cytoskeleton of the crayfish Orconectes limosus the crosslinking reagent suberic acid bis (N-hydroxysuccinimide ester) was used. 2. It was found that this crosslinking reagent can be successfully used to stabilize and isolate the microvillar cytoskeleton of crayfish photoreceptors. 3. After detergent treatment cytosolic proteins and the cell membranes were removed. 4. By the combined use of crosslinker and detergent the accessibility of antibodies or other markers to the microvillar cytoskeleton is possible. 5. This method may be useful, because at present little is known about the proteins associated with the central filament of invertebrate photoreceptors.
Schraermeyer
U
U
Institut für Biologie II (Zoologie), RWTH Aachen, Germany.
Rack
M
M
Stieve
H
H
D000818:Animals; D003400:Astacoidea / Q000648:ultrastructure*; D002458:Cell Fractionation; D003432:Cross-Linking Reagents; D003599:Cytoskeleton / Q000648:ultrastructure; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure; D010786:Photoreceptor Cells / Q000648:ultrastructure*
D003432:Cross-Linking Reagents