title: Non-coordinate expression of collagen mRNAs during carbon monoxide-induced cardiac hypertrophy.
abstract: Collagen gene expression during volume overload-induced cardiac hypertrophy was investigated in adult male rats. Hypertrophy of the left ventricle (22%) and right ventricle (37%) occurred following 27 days continuous exposure to 700 ppm carbon monoxide; hematocrit increased nearly 47%. To examine potential cellular and molecular control of restructuring in the heart, we investigated the expression of two specific procollagen mRNAs for collagen types which have different structural-functional roles [Type I (alpha-1) & Type IV]. Type I (interstitial) mRNA levels increased at least 100% relative to controls within 3 days of initial exposure to 700 ppm CO, then declined afterwards; type IV (basement membrane) mRNA levels increased more modestly at first, and increased further afterwards. The ratio of type I/type IV RNA's also increased initially, then later declined, with the greatest differences in the relative responses of type I and IV mRNAs seen in the right ventricle. These data suggest that types I and IV collagen mRNA expression is not coordinately expressed during this type of volume overload-induced hypertrophy in rat heart.
authors.affiliation: Wayne State University School of Medicine, Detroit, MI 48201.
authors.forename: D G
authors.initials: DG
authors.lastname: Penney
authors.forename: L B
authors.initials: LB
authors.lastname: Bugaisky
mesh_terms: D000818:Animals; D002248:Carbon Monoxide; D006332:Cardiomegaly / Q000139:chemically induced / Q000235:genetics*; D003094:Collagen / Q000235:genetics*; D015870:Gene Expression; D006400:Hematocrit; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D012333:RNA, Messenger; D002248:Carbon Monoxide; D003094:Collagen