Construction of a broad-host-range non-mobilizable stable vector carrying RP4 par-region.
Plasmid pXL1635 was constructed from the already segregationally stable incP-derived pRK290. Plasmid pXL1635 should be suitable for industrial and environmental uses in Gram- bacteria since (i) it contains the par fragment from RP4 which increases its stability in Pseudomonas denitrificans, a cobalamin-producing and industrially used bacterium, and (ii) the RK2 oriT has been deleted, leading to a non-mobilizable plasmid.
Crouzet
J
J
Département Biotechnologies, Rhône-Poulenc Rorer S.A., Vitry sur Seine, France.
Lévy-Schil
S
S
Cauchois
L
L
Cameron
B
B
D001483:Base Sequence; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D013045:Species Specificity