Biopsy specimen identification by detection of sex chromosomes: application of in situ hybridisation.
OBJECTIVE
To investigate the feasibility of non-radioactive in situ hybridisation (ISH) for the identification of sex-mismatched plastic embedded bone marrow biopsy specimens.


METHODS
After a suspected accidental transposition of two glycol-methacrylate embedded bone marrow specimens, in situ hybridisation with sex chromosome specific probes was performed.


RESULTS
Quantitative analysis of the hybridisation signals established unequivocably the origin of the specimens.


CONCLUSIONS
ISH is feasible on GMA embedded bone marrow specimens, and can be used for the identification of accidentally transposed specimens provided that they are of sex-matched origin.
Kibbelaar
R E
RE
Laboratory of Pathology, State University Leiden, The Netherlands.
Leenheers-Binnendijk
C F
CF
Spaander
P J
PJ
Kluin
P M
PM
D001853:Bone Marrow / Q000648:ultrastructure*; D003584:Cytological Techniques; D015342:DNA Probes; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012730:Sex Chromosomes / Q000737:chemistry*; D013048:Specimen Handling
D015342:DNA Probes