title: The mtrAB operon of Bacillus subtilis encodes GTP cyclohydrolase I (MtrA), an enzyme involved in folic acid biosynthesis, and MtrB, a regulator of tryptophan biosynthesis.
abstract: mtrA of Bacillus subtilis was shown to be the structural gene for GTP cyclohydrolase I, an enzyme essential for folic acid biosynthesis. mtrA is the first gene in a bicistronic operon that includes mtrB, a gene involved in transcriptional attenuation control of the trp genes. mtrA of B. subtilis encodes a 20-kDa polypeptide that is 50% identical to rat GTP cyclohydrolase I. Increased GTP cyclohydrolase I activity was readily detected in crude extracts of B. subtilis and Escherichia coli in which MtrA was overproduced. Biochemical evidence indicating that MtrA catalyzes dihydroneopterin triphosphate and formic acid formation from guanosine triphosphate is presented. It was also shown that mtrB of B. subtilis encodes a 6-kDa polypeptide. Expression of mtrB is sufficient for transcriptional attenuation control of the B. subtilis trp gene cluster in Escherichia coli. Known interrelationships between genes involved in folic acid and aromatic amino acid biosynthesis in B. subtilis are described.
authors.affiliation: Department of Biological Sciences, Stanford University, California 94305.
authors.forename: P
authors.initials: P
authors.lastname: Babitzke
authors.forename: P
authors.initials: P
authors.lastname: Gollnick
authors.forename: C
authors.initials: C
authors.lastname: Yanofsky
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005492:Folic Acid / Q000378:metabolism; D006136:GTP Cyclohydrolase / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014364:Tryptophan / Q000096:biosynthesis
chemical_list: D014364:Tryptophan; D005492:Folic Acid; D006136:GTP Cyclohydrolase