title: The molecular mechanism of photoporphyrin (YHPD)'s photosensitization--laser Raman spectroscopic study of microcosmic and photosensitive damage of YHPD to protein.
abstract: Laser Raman spectroscopy was used to investigate the microcosmic and photosensitive damage of YHPD to lysozyme, of which the three-dimensional structure has been elucidated. The experimental results shown by various damages of the main-chain and side-chain of lysozyme are as follows: (i) Phe and Cys are also damaged by photosensitization of YHPD, except for Trp, Tyr, Met, 1/2Cys and His; (ii) the order of the photosensitized sensitivity of various groups of these amino acids have been described; (iii) Trp and Tyr buried in the three-dimensional structure of the protein are damaged very greatly, and (iv) the main-chain conformation of the protein has changed considerably, such as a decrease in orderly structure (alpha-helix, beta-sheet and beta-turn) and a simultaneous increase in random
authors.affiliation: Institute of Biophysics, Academia Sinica, Beijing, PRC.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Y M
authors.initials: YM
authors.lastname: Xu
mesh_terms: D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins / Q000494:pharmacology*; D008968:Molecular Conformation; D009113:Muramidase / Q000187:drug effects*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D013059:Spectrum Analysis, Raman
chemical_list: D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative; D009113:Muramidase