Expression and ligand-binding function of the integrin alpha 4 beta 1 (VLA-4) on neural-crest-derived tumor cell lines.
Human neural-crest-derived tumor cell lines, including three neuroblastomas, an astrocytoma, a glioblastoma, a rhabdomyosarcoma and a melanoma were screened for the expression of the integrin alpha 4 beta 1 (VLA-4). The neuroblastomas IMR-32 and SK-N-SH, the astrocytoma 131-INI, the glioblastoma Fogerty and the rhabdomyosarcoma TE-671 expressed alpha 4 beta 1 as determined by cytofluorometry and immunoprecipitation. Another neuroblastoma line, LA-N-1, did not express alpha 4 beta 1. Analysis of immunoprecipitated alpha 4 beta 1 showed that the alpha 4 subunit from the various cell types differed in relative molecular weight (M(r)). The variability in the observed M(r) could be accounted for by differences in the levels of N-linked glycosylation. The observed variability in M(r) did not appear to affect function since intact cells and solubilized alpha 4 beta 1 bound to a synthetic peptide identical in sequence to the CS-1 region of the alternatively spliced IIICS domain of fibronectin, a known alpha 4 beta 1 ligand.
Bednarczyk
J L
JL
Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
McIntyre
B W
BW
D000595:Amino Acid Sequence; D001254:Astrocytoma / Q000378:metabolism / Q000503:physiopathology / Q000648:ultrastructure; D002846:Chromatography, Affinity; D005910:Glioma / Q000378:metabolism / Q000503:physiopathology / Q000648:ultrastructure; D006031:Glycosylation; D006801:Humans; D039041:Integrin alpha4beta1; D016023:Integrins / Q000378:metabolism / Q000502:physiology*; D008545:Melanoma / Q000378:metabolism / Q000503:physiopathology / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009423:Nervous System Neoplasms / Q000378:metabolism / Q000503:physiopathology* / Q000648:ultrastructure; D009432:Neural Crest / Q000473:pathology*; D009447:Neuroblastoma / Q000378:metabolism / Q000503:physiopathology / Q000648:ultrastructure; D011233:Precipitin Tests; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000502:physiology; D012208:Rhabdomyosarcoma / Q000378:metabolism / Q000503:physiopathology / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D039041:Integrin alpha4beta1; D016023:Integrins; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic