The effect of desferrioxamine and ferric ammonium citrate on the uptake of iron by the membrane iron-binding component of human melanoma cells.
The effect of ferric ammonium citrate (FAC) and desferrioxamine (DFO) on membrane iron and transferrin uptake have been investigated using SK-MEL-28 human melanoma cells which express the membrane-bound transferrin homologue, melanotransferrin, at high concentrations. Exposure of melanoma cells to DFO increased membrane non-Tf-bound Fe uptake (putative melanotransferrin Fe-binding sites), suggesting upregulation of the membrane Fe-binding component. However, exposure to FAC did not result in down-regulation. Indeed, an increase in non-Tf-bound membrane Fe was apparent. Results suggested that non-Tf-bound membrane Fe uptake occurred by two processes corresponding to the specific and non-specific mechanisms of Fe uptake from Tf described previously (Richardson, D.R. and Baker, E. (1990) Biochim. Biophys. Acta 1053, 1-12).
Richardson
D R
DR
Department of Physiology, University of Western Australia, Nedlands.
Baker
E
E
D002462:Cell Membrane / Q000378:metabolism; D003676:Deferoxamine / Q000494:pharmacology*; D005290:Ferric Compounds / Q000494:pharmacology*; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008545:Melanoma; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D014168:Transferrin / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D005290:Ferric Compounds; D000644:Quaternary Ammonium Compounds; D014168:Transferrin; D007501:Iron; D003676:Deferoxamine; C013531:ferric ammonium citrate