Rhodanese and ALA-S in mammary tumor and liver from normal and tumor-bearing mice.
1. Basal levels and allyl-isopropylacetamide (AIA) or veronal induced levels of delta-amino-levulinate synthetase (ALA-S), cytoplasmic and mitochondrial rhodanese were determined in tumor (T) and liver of both normal mice (NM) and T-bearing mice (TBM). 2. Rhodanese tumoral mitochondrial levels were higher than the hepatic normal mitochondrial fraction, while the cytoplasmic activity was nearly equal in all sources. 3. In neither case was the activity of tumoral ALA-S and rhodanese altered by any of the porphyrinogenic drugs. 4. Mitochondrial and cytoplasmic rhodanese activity was also measured in tumor and liver of TBM at different intervals after transplantation. We concluded that the behaviour of rhodanese is a property inherent to the tissue and not one attained with time.
Navone
N M
NM
Centro de Investigaciones sobre Porfirinas y Porfirias, (CONICET, FCEN, UBA), Buenos Aires, Argentina.
Vázquez
E S
ES
Polo
C F
CF
Batlle
A M
AM
D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis / Q000378:metabolism*; D000502:Allylisopropylacetamide / Q000494:pharmacology; D000818:Animals; D001462:Barbital / Q000494:pharmacology; D003593:Cytoplasm / Q000201:enzymology; D004790:Enzyme Induction / Q000187:drug effects; D008099:Liver / Q000201:enzymology; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000201:enzymology; D013884:Thiosulfate Sulfurtransferase / Q000096:biosynthesis / Q000378:metabolism*
D000502:Allylisopropylacetamide; D001462:Barbital; D000624:5-Aminolevulinate Synthetase; D013884:Thiosulfate Sulfurtransferase