title: Nucleotide sequence analyses of peanut stunt cucumovirus RNAs 1 and 2.
abstract: The nucleotide sequences of the RNAs 1 and 2 of the peanut stunt virus strain J (PSV-J) were determined and compared with those of the cucumber mosaic virus strain Y (CMV-Y, subgroup I), strain Q (CMV-Q, subgroup II) and the tomato aspermy virus strain V (TAV-V) at both the nucleotide and protein levels. RNA 1 of PSV-J consists of 3355 nucleotides (nt) and has one large open reading frame (ORF) which can encode the putative 1a protein of Mr 112025. PSV-J RNA 1 and the 1a protein are 65 to 73% identical to those of CMV-Y and -Q, and 65 to 69% to those of TAV-V, RNA 2 of PSV-J contains 2946 nt and also has one large ORF which can encode the putative 2a protein of Mr 93575. For RNA 2 and the 2a protein, identities between PSV-J and two strains of CMV are calculated to be 53 to 61%. When compared with TAV-V, the same degree of similarity as seen with CMVs is observed. The 1a protein has the consensus sequences found in some helicases and methyltransferases and the 2a protein includes a sequence which exists in several RNA-dependent RNA polymerases.
authors.affiliation: Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Karasawa
authors.forename: K
authors.initials: K
authors.lastname: Nakaho
authors.forename: T
authors.initials: T
authors.lastname: Kakutani
authors.forename: Y
authors.initials: Y
authors.lastname: Minobe
authors.forename: Y
authors.initials: Y
authors.lastname: Ehara
mesh_terms: D000595:Amino Acid Sequence; D010367:Arachis / Q000382:microbiology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010942:Plant Viruses / Q000235:genetics*; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000737:chemistry*; D016364:Reading Frames; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004277:DNA, Single-Stranded; D012367:RNA, Viral