Oxytocin, amino acid and monoamine release in the region of the medial preoptic area and bed nucleus of the stria terminalis of the sheep during parturition and suckling.
Microdialysis sampling was used to measure the release of oxytocin (OXY) and monoamine and amino acid transmitters from the region of the medial preoptic area (MPOA) and the bed nucleus of the stria terminalis (BNST) during parturition and suckling in sheep. Results showed that OXY and gamma-aminobutyric acid release increased in both the MPOA and BNST during parturition and suckling. Noradrenaline (NA) release increased in both structures during parturition but not during suckling. Dopamine (DA) release increased in the MPOA and decreased in the BNST during both parturition and suckling. Aspartate release increased in the MPOA during parturition, and the BNST during suckling, and glutamate release increased in the MPOA and BNST at parturition and only in the BNST during suckling. No changes in the release of serotonin or taurine occurred in these structures during parturition or suckling. In a further experiment on 6 estrogen-primed sheep, OXY (10 micrograms/ml) was infused into the MPOA via bilaterally placed microdialysis probes. This treatment inhibited rejection behavior towards lambs, but did not activate positive maternal responses. These OXY infusions also stimulated release of NA. These results show that complex patterns of neurochemical release occur in two closely related areas of the brain, the BNST and MPOA, during parturition when maternal behavior is stimulated. However, while these patterns of release are similar in the two structures, particularly at birth when maternal behavior is stimulated, they are not identical during labor contractions and suckling. The release of oxytocin within the MPOA during parturition may be important for stimulating a reduction in aggression towards lambs, although this action might be mediated via the effect of OXY on NA release.
Kendrick
K M
KM
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
Keverne
E B
EB
Hinton
M R
MR
Goode
J A
JA
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D007263:Infusions, Parenteral; D007743:Labor, Obstetric / Q000502:physiology*; D007774:Lactation / Q000502:physiology*; D009638:Norepinephrine / Q000378:metabolism*; D010052:Ovariectomy; D010121:Oxytocin / Q000008:administration & dosage / Q000494:pharmacology; D011247:Pregnancy; D011301:Preoptic Area / Q000187:drug effects / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism; D012756:Sheep; D013238:Stereotaxic Techniques; D013654:Taurine / Q000378:metabolism; D013787:Thalamic Nuclei / Q000187:drug effects / Q000502:physiology*; D014590:Uterine Contraction; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000596:Amino Acids; D013654:Taurine; D001224:Aspartic Acid; D012701:Serotonin; D004958:Estradiol; D010121:Oxytocin; D005680:gamma-Aminobutyric Acid; D004298:Dopamine; D009638:Norepinephrine