Biosynthesis of cholic acid accelerated by diabetes: its mechanism and effect of vanadate administration.
[7 beta-3H]3 alpha,7 alpha-Dihydroxy-5 beta-cholestane was infused into the femoral vein of bile-fistula rats. The main metabolites in bile were cholic, chenodeoxycholic, and alpha-muricholic acids. Cholic acid accounted for only 7.5% of the total biliary [3H]bile acids in the normal rats, but 51.8% in the diabetic rats. This increased proportion of cholic acid was partially cancelled by treatment of the diabetic rats with insulin (29.8%) or vanadate (28.8%). These results clearly confirm that an alternative biosynthetic pathway of cholic acid via 3 alpha,7 alpha-dihydroxy-5 beta-cholestane is accelerated by diabetes, and indicate that vanadate has an insulin-like effect on the formation of cholic acid in an insulin-deficient state.
Kimura
K
K
Department of Biochemistry, Faculty of Medicine, Tottori University, Japan.
Ogura
Y
Y
Ogura
M
M
D000818:Animals; D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D019826:Cholic Acid; D002793:Cholic Acids / Q000096:biosynthesis*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014638:Vanadates / Q000494:pharmacology*
D001647:Bile Acids and Salts; D002793:Cholic Acids; D014638:Vanadates; D019826:Cholic Acid