A novel probe for human DNA fingerprinting based on chi-like sequences.
A synthetic oligodeoxyribonucleotide (oligo) containing crossover initiating hotspot-like sequences was designed on the assumption that hypervariability is partly due to the presence of molecular signals which promote recombination. This oligo, when used as a probe for human DNA fingerprinting, generated individual-specific DNA band patterns. The probability of two unrelated individuals having the same DNA band pattern, using this probe, was estimated to be 1.9 x 10(-13).
Ehtesham
N Z
NZ
National Institute of Immunology, New Delhi, India.
Das
A
A
Hasnain
S E
SE
D001483:Base Sequence; D016172:DNA Fingerprinting; D015342:DNA Probes / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides