title: Immunohistochemical localization of fibronectin-like protein in the inner ear of the developing gerbil and rat.
abstract: Immunohistochemistry was used to demonstrate the distribution of fibronectin-like protein within the developing inner ear of two species of altricial rodents: gerbils and rats. While there were temporal differences between the two species, the developmental sequence of immunostaining was virtually identical. Most notably, in rats from embryonic day 18 through day 1 postpartum, and in gerbils from birth through day 4 postpartum, intense, discrete fibronectin-like immunoreactivity was observed in the cochlea immediately beneath the inner and outer hair cells, sites of active auditory nerve fiber growth and nerve-hair cell synaptogenesis at these ages. The results suggest that fibronectin is appropriately positioned spatially and temporally to play a significant role in promoting, guiding and/or maintaining neural innervation within the developing organ of Corti. The temporo-spatial pattern of immunostaining in Schwann cells and auditory (VIIIth cranial) nerve neurons implies that fibronectin also plays a significant role in the early formation of myelin. In non-neural elements of the cochlea, fibronectin is a major structural component within the basilar membrane at all of the developmental stages investigated.
authors.affiliation: Department of Surgery, University of California, San Diego, School of Medicine, La Jolla.
authors.forename: N K
authors.initials: NK
authors.lastname: Woolf
authors.forename: F J
authors.initials: FJ
authors.lastname: Koehrn
authors.forename: A F
authors.initials: AF
authors.lastname: Ryan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003051:Cochlea / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D007758:Ear, Inner / Q000196:embryology* / Q000254:growth & development; D005353:Fibronectins / Q000378:metabolism*; D005849:Gerbillinae / Q000196:embryology* / Q000254:growth & development; D006198:Hair Cells, Auditory / Q000378:metabolism; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism; D051381:Rats / Q000196:embryology* / Q000254:growth & development; D013569:Synapses / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D005353:Fibronectins