Non-essential repeats in the promoter region of a Brassica rapa acyl carrier protein gene expressed in developing embryos.
A genomic clone of an acyl carrier protein gene (Bcg4-4) which is highly expressed in developing embryos of Brassica rapa was isolated and sequenced. The promoter and transcription terminator regions of Bcg4-4 were used to express a beta-glucuronidase reporter gene in transgenic rapeseed. Deletion of repeated domains in the promoter region did not lower beta-glucuronidase expression in seeds.
Scherer
D
D
Calgene, Inc., Davis, CA 95616.
Sato
A
A
McCarter
D W
DW
Radke
S E
SE
Kridl
J C
JC
Knauf
V C
VC
D000213:Acyl Carrier Protein / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017343:Genes, Plant; D015698:Genomic Library; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012639:Seeds / Q000502:physiology; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D000213:Acyl Carrier Protein; D004247:DNA; D005966:Glucuronidase