title: The role of the exocyclic amino groups of conserved purines in hammerhead ribozyme cleavage.
abstract: A series of 2 stranded hammerhead ribozymes has been synthesized in which single conserved adenosine residues have been replaced by nebularine or single guanosine residues by inosine. Comparison of the rates of trans-cleavage for the modified structures suggests the presence of interstrand non-Watson-Crick hydrogen bonding interactions including a GA:AG double mismatch. The exocyclic amino group of one of the guanosine residues is essential for cleavage.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: G
authors.initials: G
authors.lastname: Slim
authors.forename: M J
authors.initials: MJ
authors.lastname: Gait
mesh_terms: D000241:Adenosine / Q000737:chemistry; D000588:Amines / Q000737:chemistry*; D001483:Base Sequence; D006151:Guanosine / Q000737:chemistry; D007288:Inosine / Q000737:chemistry; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011684:Purine Nucleosides / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D012263:Ribonucleosides / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D000588:Amines; D011684:Purine Nucleosides; D016337:RNA, Catalytic; D012263:Ribonucleosides; D006151:Guanosine; D007288:Inosine; C005248:nebularine; D000241:Adenosine