Influence of inhibitors of lipid biosynthesis on the production of avermectins in Streptomyces avermitilis.
Streptomyces avermitilis was grown in the presence of four inhibitors of fatty acid biosynthesis (p-chlorophenoxyacetic, DL-2-(p-chlorophenoxy)-propionic, 2-(p-chlorophenoxy)-2-methyl-propionic and p-fluorophenoxyacetic acids). The production of total lipids was inhibited and the cellular content of individual types of fatty acids was altered by these compounds. The anteiso-acid content decreased by up to one-half, whereas the content of iso-odd acids increased 10-fold in the presence of inhibitors. The decrease in anteiso-acids was accompanied by increased production of avermectins having a s-butyl group in the side chain (type 'a' avermectins). It was concluded that the common precursor, 2-methylbutyryl-CoA, was diverted from the biosynthetic pathway of fatty acids to that of avermectins.
Rezanka
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T
Institute of Microbiology, Prague, Czechoslovakia.
Míková
H
H
Jurková
M
M
D000960:Hypolipidemic Agents / Q000494:pharmacology*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D000960:Hypolipidemic Agents; D008055:Lipids; D014280:Triglycerides; D007559:Ivermectin; C019264:avermectin