Preliminary evidence for a postsynaptic action of beta-bungarotoxin in mammalian skeletal muscle.
Two hours after treatment with beta-bungarotoxin (0.34-0.4 microM), when there was complete neuromuscular block, the peak contracture response to 50 microM succinylcholine was significantly reduced by about 35% in the mouse phrenic nerve-diaphragm preparation. Additionally, significant phospholipase A2 activity was detected on primary cell cultures from skeletal muscle which were incubated for 2 hr with concentrations of beta-bungarotoxin greater than or equal to 0.1 microM. Thus, beta-bungarotoxin appears to have pharmacologically and biochemically detectable postsynaptic actions in mammalian muscle systems.
Storella
R J
RJ
Department of Anesthesiology, Hahnemann University, Philadelphia, PA 19102.
Schouchoff
A L
AL
Fujii
M
M
Hill
J
J
Fletcher
J E
JE
Jiang
M S
MS
Smith
L A
LA
D000818:Animals; D002038:Bungarotoxins / Q000633:toxicity*; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D009132:Muscles / Q000187:drug effects* / Q000201:enzymology; D009469:Neuromuscular Junction / Q000187:drug effects*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D013390:Succinylcholine / Q000494:pharmacology
D002038:Bungarotoxins; D010741:Phospholipases A; D054467:Phospholipases A2; D013390:Succinylcholine