Somatic cell mapping of the bovine interferon-alpha receptor.
The bovine interferon-alpha receptor (BoIFN-alpha R) mediates the activity of bovine IFN-alpha s and IFN-beta. In addition, human IFN-alpha s have uniformly high biological activity on bovine cells. A 32P-labeled derivative of human recombinant IFN-alpha A (HuIFN-alpha A-P1) binds well and can form a characteristic 130-kDa complex on bovine cells, but not on hamster cells. We have, therefore, analyzed the binding and covalent crosslinking of [32P]HuIFN-alpha A-P1 to a panel of bovine-hamster somatic cell hybrids. Binding to several bovine-hamster hybrid cell lines was strong (about 30-50% of that seen with bovine MDBK cells) and specific. The binding correlated uniquely with bovine syntenic group U10. In several of the hybrid lines, the ability of human IFN-alpha B to enhance the expression of endogenous MHC class I molecules correlated with the binding results. We thus conclude that the bovine IFN-alpha R structural gene (locus designation IFNAR) localizes to syntenic group U10. This group includes a number of other genes whose homologs map to human Chromosome (Chr) 21.
Langer
J A
JA
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854-5635.
Puvanakrishnan
R
R
Womack
J E
JE
D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping / Q000379:methods; D006224:Cricetinae; D006822:Hybrid Cells; D016898:Interferon-alpha; D011869:Radioligand Assay; D011971:Receptors, Immunologic / Q000235:genetics*; D017471:Receptors, Interferon
D016898:Interferon-alpha; D011971:Receptors, Immunologic; D017471:Receptors, Interferon