Tetrahydroaminoacridine and related compounds interfere with fura-2 and indo-1.
In earlier experiments, using a fluorimetric method (fura-2), we found what seemed to be a decreased cytoplasmic [Ca2+] in neuroblastoma cells when 1,2,3,4-tetrahydro-9-aminoacridine (THA) was added, but discovered by repeating our work under cell-free conditions, that THA affected the Ca(2+)-fura-2 signal. The present study aimed at investigating the previously observed interference of THA with fura-2. 1 mM THA completely inhibits fluorescence of fura-2 and another Ca(2+)-indicator, indo-1, 1 microM each (apparent IC50 = 40 microM), presumably by absorbing excitation light.
Järlebark
L
L
Unit of Neurochemistry and Neurotoxicology, University of Stockholm, Sweden.
Heilbronn
E
E
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D016257:Fura-2 / Q000032:analysis*; D007211:Indoles / Q000032:analysis*; D051381:Rats; D013619:Tacrine / Q000494:pharmacology*
D007211:Indoles; D013619:Tacrine; D015761:4-Aminopyridine; C048960:indo-1; D016257:Fura-2