title: Induction of a putative laminin-binding protein of Streptococcus gordonii in human infective endocarditis.
abstract: There is evidence to suggest that the virulence of Streptococcus strains in infective endocarditis might be due to the expression of binding sites for the extracellular matrix proteins of damaged valves. In this communication, we draw attention to one laminin-binding protein from a strain of Streptococcus gordonii isolated from a patient with human endocarditis. This 145-kDa protein was found on the cell wall of the bacterium. The level of expression of this binding protein might be regulated by the presence of extracellular matrix proteins: the protein was lacking after in vitro selection of laminin, collagen I, and fibronectin nonbinding variants, and it was recovered after growth of the variants when laminin or collagen I was added to the growth medium. It was also missing after 10 subcultures in minimal medium, indicating some positive control. Furthermore, the 145-kDa protein was recognized as a major antigen by sera from patients treated for streptococcal infective endocarditis, while sera from patients with valvulopathies gave only slight recognition, suggesting an increase of the expression of this protein during infective endocarditis. It was also shown that the 145-kDa protein carried a collagen I-like determinant detected with anti-human collagen I antibodies.
authors.affiliation: Department of Pathology, Centre National de la Recherche Scientifique URA 1459, Institut Pasteur of Lyon, France.
authors.forename: P
authors.initials: P
authors.lastname: Sommer
authors.forename: C
authors.initials: C
authors.lastname: Gleyzal
authors.forename: S
authors.initials: S
authors.lastname: Guerret
authors.forename: J
authors.initials: J
authors.lastname: Etienne
authors.forename: J A
authors.initials: JA
authors.lastname: Grimaud
mesh_terms: D003094:Collagen / Q000276:immunology / Q000378:metabolism; D003429:Cross Reactions; D004697:Endocarditis, Bacterial / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D008970:Molecular Weight; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D017474:Receptors, Laminin; D013290:Streptococcal Infections / Q000378:metabolism*; D013298:Streptococcus sanguis / Q000378:metabolism*
chemical_list: D007797:Laminin; D011971:Receptors, Immunologic; D017474:Receptors, Laminin; D003094:Collagen