Isolation of elafin and elastase-specific inhibitor (ESI) from bronchial secretions. Evidence of sequence homology and immunological cross-reactivity.
Evidence is presented that the elastase-specific inhibitor of Mr 2500 (Sallenave, J.-M. & Ryle, A.P. (1991) Biol. Chem. Hoppe-Seyler 372, 13-21) is a biologically active fragment of a larger molecule described in the skin of patients with psoriasis (Wiedow, O., Shroder, J.-M., Gregory, H., Young, J.A. & Christophers, E. (1990) J. Biol. Chem. 265, 14791-14795) which the authors called elafin. We also describe the purification of the complete elafin molecule from bronchial secretions from a patient suffering from bronchial carcinoma, thus showing that the elafin, like the mucus proteinase inhibitor (MPI), is not of single origin but is probably a marker of inflammation (chronic obstructive pulmonary diseases, psoriasis...) present in different tissues.
Sallenave
J M
JM
Department of Biochemistry, Edinburgh University Medical School.
Marsden
M D
MD
Ryle
A P
AP
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002283:Carcinoma, Bronchogenic / Q000737:chemistry; D002853:Chromatography, Liquid; D003429:Cross Reactions; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry; D008969:Molecular Sequence Data; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D015842:Serine Proteinase Inhibitors / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D013183:Sputum / Q000737:chemistry
D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors; D010196:Pancreatic Elastase