Self-association of kangaroo and chicken insulins, chicken despentapeptide insulin and deamidated insulin.
Intact and modified metal-free insulins from kangaroo, chicken and pig have been purified by HPLC and studied by equilibrium sedimentation at pH 7. The aggregation of deamidated pig insulin is lowered significantly, consistent with its modified electrostatic charge. Native kangaroo and chicken insulins self-associate to the same extent as pig and cow insulins under these conditions. Despentapeptide chicken insulin does not self-associate at pH 7.
Jeffrey
P D
PD
Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberro.
Treacy
G B
GB
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000302:isolation & purification; D007700:Kinetics; D046911:Macromolecular Substances; D007614:Macropodidae; D008970:Molecular Weight; D013552:Swine
D007328:Insulin; D046911:Macromolecular Substances