Antibodies to beta 1-integrins cause alterations of aortic vasculogenesis, in vivo.
Vasculogenesis is the de novo formation of blood vessels from mesoderm. This process occurs very early in development and provides a convenient system for studying morphogenesis in higher vertebrates. The cell-extracellular matrix (ECM) interactions that occur during dorsal aortic vasculogenesis were examined using the monoclonal antibody, CSAT, a reagent known to neutralize the ligand-binding activity of avian beta 1-integrins. We injected CSAT into quail embryos during a period of active vasculogenesis (4-10 somites). The CSAT antibodies, but not controls, had a marked and reproducible effect on aortic vessel formation. Vasculogenesis appeared to be arrested at the stage when slender cord-like assemblies of angioblasts rearrange to form tubules. Indeed, aortic primordia near the site of CSAT injection did not form patent vessels.
Drake
C J
CJ
Department of Anatomy and Cell Biology, University of Virginia, Charlottesville 22908.
Davis
L A
LA
Little
C D
CD
D000818:Animals; D000911:Antibodies, Monoclonal; D001011:Aorta / Q000002:abnormalities / Q000196:embryology*; D003370:Coturnix; D004707:Endoderm / Q000502:physiology; D007074:Immunoglobulin G; D016023:Integrins / Q000276:immunology / Q000502:physiology*; D008648:Mesoderm / Q000502:physiology; D009024:Morphogenesis
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D016023:Integrins