Partial purification and characterization of a factor for the enhancement of colony formation in vitro by myeloid progenitor cells.
We have purified a factor, hematopoietic promoting factor (HPF), from porcine kidney extract (PKE), which exhibits a promoting activity on granulocyte/macrophage (GM) colony and burst-forming-unit-erythroid (BFU-E)-derived colony formation by progenitors from murine bone marrow cells in vitro. The addition of HPF resulted in an enhancement of the GM colonies as well as BFU-E-derived colonies, but did not enhance the colony-forming-unit-erythroid (CFU-E)-derived colony formation. HPF was added to the BFU-E cultures together with cytokines, such as recombinant murine interleukin-3 (IL-3), recombinant murine GM colony-stimulating-factor (GM-CSF) and recombinant human G-CSF, which have all been shown to enhance BFU-E growth. The combination of HPF plus these cytokines resulted in an enhancement of benzidine negative colony formation in comparison to the case of each cytokine alone; however, no increase was found on BFU-E colony formation. HPF is able to enhance the granulopoiesis and erythropoiesis in vitro. And the synergistic activity of HPF is significantly affected by the presence of cytokines in the cultures.
Kashiwakura
I
I
Hokkaido Institute of Pharmaceutical Sciences, Otaru, Japan.
Murakami
M
M
Hayase
Y
Y
Takagi
Y
Y
D000818:Animals; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000302:isolation & purification / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects; D016298:Hematopoietic Cell Growth Factors / Q000302:isolation & purification* / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D014020:Tissue Extracts / Q000302:isolation & purification* / Q000494:pharmacology
D016298:Hematopoietic Cell Growth Factors; D014020:Tissue Extracts; D016178:Granulocyte-Macrophage Colony-Stimulating Factor