A competitive ELISA for lipoprotein(a).
A competitive ELISA for lipoprotein(a) (Lp(a)) is described. The method uses a commercially available polyclonal anti-Lp(a) antibody and an IgG biotinstreptavidin-horseradish peroxidase detection system. The method is simple and robust with an assay sensitivity of 0.7 ng/well (1.4 micrograms/l). The antibody cross-reactivity was 0.14% against LDL and 0.70% against plasminogen. The coefficients of variation obtained with control sera of 266 and 552 mg/l were: 5.0% and 4.6% (n = 6), respectively for the intraassay; and 10.8% and 9.5% (n = 16), respectively for the interassay. The method showed an excellent correlation with a commercial immunoradiometric assay (IRMA), y (ELISA) = 0.94x (IRMA) - 8, (r = 0.98). A recovery study in which a 200 mg/L standard and four plasma samples were diluted with different proportions of a low plasma sample, gave linear relationships and also confirmed the specificity of the antibody.
Yeo
K H
KH
Department of Clinical Biochemistry, Christchurch Hospital, New Zealand.
Walmsley
T A
TA
Owen
M C
MC
George
P M
PM
D001667:Binding, Competitive; D003327:Coronary Disease / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015592:Immunoradiometric Assay; D017270:Lipoprotein(a); D008074:Lipoproteins / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood
D017270:Lipoprotein(a); D008074:Lipoproteins; D008077:Lipoproteins, LDL