The monoclonal antibody MEL-14 can block lymphocyte migration into a site of chronic inflammation.
The effect of MEL-14, a monoclonal antibody which binds to the lymphocyte homing receptor (MEL-14 Ag) on lymphocytes for peripheral lymph node (PLN) high endothelial venules (HEV), was investigated on lymphocyte migration into a delayed-type hypersensitivity (DTH)-like lesion produced by sensitization and challenge to Bordetella pertussis vaccine (BPV). Pretreatment of lymphocytes with saturating concentrations of MEL-14 caused a highly significant inhibition of lymphocyte migration into the chronically inflamed site and PLN. This finding suggests that lymphocyte migration into the BPV-induced site of chronic inflammation may be regulated by the same mechanism as lymphocyte migration into the PLN. It further indicates that the ligand for the MEL-14 Ag adhesion molecule, recognized by lymphocyte which home to the PLN, may also be expressed on HEV-like vessels at sites of BPV-induced chronic inflammation.
Dawson
J
J
Department of Pharmacology, Royal Veterinary College, Hatfield, London.
Sedgwick
A D
AD
Edwards
J C
JC
Lees
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D015703:Antigens, CD / Q000502:physiology; D001790:Blood Physiological Phenomena; D002464:Cell Migration Inhibition; D002465:Cell Movement / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006649:Histocompatibility Antigens / Q000502:physiology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007249:Inflammation / Q000276:immunology*; D017493:Leukocyte Common Antigens; D008099:Liver / Q000502:physiology; D008198:Lymph Nodes / Q000276:immunology / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010567:Pertussis Vaccine / Q000276:immunology; D010581:Peyer's Patches / Q000502:physiology; D016201:Receptors, Lymphocyte Homing / Q000502:physiology*; D013154:Spleen / Q000502:physiology; D013950:Thymus Gland / Q000502:physiology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006649:Histocompatibility Antigens; D010567:Pertussis Vaccine; D016201:Receptors, Lymphocyte Homing; D017493:Leukocyte Common Antigens