title: Localization of proteins forming the outer surface of isolated metaphase chromosomes.
abstract: The outer surface of isolated metaphase chromosomes has been investigated by a method of thermally activated tritium labelling. We show that both chromosomal proteins and DNA are tritium-labelled. Fractionation of the chromosomal proteins reveals that scaffold proteins are the most labelled in condensed and EDTA-decondensed chromosomes. Exposition of some scaffold proteins on the outer surface of metaphase chromosomes is suggested.
authors.affiliation: Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences.
authors.forename: N D
authors.initials: ND
authors.lastname: Belyaev
authors.forename: V G
authors.initials: VG
authors.lastname: Budker
authors.forename: V A
authors.initials: VA
authors.lastname: Dubrovskaya
authors.forename: A A
authors.initials: AA
authors.lastname: Kim
authors.forename: E V
authors.initials: EV
authors.lastname: Kiseleva
authors.forename: V N
authors.initials: VN
authors.lastname: Sidorov
mesh_terms: D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D002875:Chromosomes; D005347:Fibroblasts / Q000378:metabolism; D008677:Metaphase; D051379:Mice; D008854:Microscopy, Electron; D014316:Tritium
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D014316:Tritium