title: Production of porcine parvovirus empty capsids with high immunogenic activity.
abstract: The VP2 gene of porcine parvovirus was cloned in the baculovirus system and expressed in insect cells. The resulting product was present in high yield. It self-assembled into particles which were structurally and antigenically indistinguishable from regular PPV capsids. A high degree of purity of the recombinant capsids was obtained by ammonium sulphate precipitation of cell lysates. These virus-like particles were used as antigen in the immunization of two pigs. The pigs elicited an immune response which, when assayed by standard serological techniques, was identical to that of a commercial vaccine. The amount of recombinant antigen needed in a vaccine dose was only 3 micrograms in a primary dose and 1.5 micrograms in the booster.
authors.affiliation: Inmunologia y Genetica Aplicada SA (Ingenasa), Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Martínez
authors.forename: K
authors.initials: K
authors.lastname: Dalsgaard
authors.forename: J A
authors.initials: JA
authors.lastname: López de Turiso
authors.forename: E
authors.initials: E
authors.lastname: Cortés
authors.forename: C
authors.initials: C
authors.lastname: Vela
authors.forename: J I
authors.initials: JI
authors.lastname: Casal
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000276:immunology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors / Q000235:genetics; D006386:Hemagglutination Tests; D007114:Immunization; D008969:Molecular Sequence Data; D010321:Parvoviridae / Q000235:genetics*; D011994:Recombinant Proteins / Q000302:isolation & purification; D012641:Selection, Genetic; D013552:Swine; D014162:Transfection / Q000235:genetics
chemical_list: D004279:DNA, Viral; D011994:Recombinant Proteins