Two-dimensional model of calcium waves reproduces the patterns observed in Xenopus oocytes.
Biological excitability enables the rapid transmission of physiological signals over distance. Using confocal fluorescence microscopy, we previously reported circular, planar, and spiral waves of Ca2+ in Xenopus laevis oocytes that annihilated one another upon collision. We present experimental evidence that the excitable process underlying wave propagation depends on Ca2+ diffusion and does not require oscillations in inositol (1,4,5)trisphosphate (IP3) concentration. Extending an existing ordinary differential equation (ODE) model of Ca2+ oscillations to two spatial dimensions, we develop a partial differential equation (PDE) model of Ca2+ excitability. The model assumes that cytosolic Ca2+ couples neighboring Ca2+ release sites. This simple PDE model qualitatively reproduces our experimental observations.
Girard
S
S
Department of Pharmacology, Mayo Clinic and Foundation, Rochester, Minnesota.
Lückhoff
A
A
Lechleiter
J
J
Sneyd
J
J
Clapham
D
D
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004058:Diffusion; D005260:Female; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D008433:Mathematics; D008954:Models, Biological; D009865:Oocytes / Q000378:metabolism*; D014982:Xenopus laevis
D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium