Hepatocyte growth factor (HGF) mediates the sustained formation of 1,2-diacylglycerol via phosphatidylcholine-phospholipase C in cultured rat hepatocytes.
The addition of hepatocyte growth factor (HGF) to rat hepatocytes in primary culture resulted in the formation of inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and 1,2-diacylglycerol (DG) by a phosphoinositide-specific phospholipase C (PI-PLC). DG showed a biphasic increase; the first phase, corresponding with the peak of Ins(1,4,5)P3 and a second larger and prolonged phase. The HGF stimulates the phosphatidylcholine (PC)-derived prolonged DG formation by a phospholipase C pathway (PC-PLC) but not by a phospholipase D pathway. HGF also was found to elicit [Ca2+] oscillations which may be associated with the prolonged DG production from PC via the PC-PLC phospholipase C pathway.
Osada
S
S
Second Department of Surgery, Gifu University School of Medicine, Japan.
Nakashima
S
S
Saji
S
S
Nakamura
T
T
Nozawa
Y
Y
D000818:Animals; D002478:Cells, Cultured; D004075:Diglycerides / Q000378:metabolism*; D006133:Growth Substances / Q000378:metabolism*; D017228:Hepatocyte Growth Factor; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015398:Signal Transduction; D010738:Type C Phospholipases / Q000378:metabolism*
C011439:1,2-diacylglycerol; D004075:Diglycerides; D006133:Growth Substances; D010713:Phosphatidylcholines; D017228:Hepatocyte Growth Factor; D010738:Type C Phospholipases