title: Sequential intracoelomic and intrabursal immunization of chickens with inactivated Mycoplasma gallisepticum bacterin and iota carrageenan adjuvant.
abstract: Chickens immunized by sequential intracoelomic (analogous to intraperitoneal route in mammals) and intrabursal (i.c./i.b.) routes with inactivated Mycoplasma gallisepticum (MG) bacterin mixed with 0.2% iota carrageenan (iCGN) as an adjuvant were resistant to airsacculitis induced by a subsequent aerosol challenge with virulent R strain MG. In contrast, immunization by the i.c./i.b. routes with inactivated bacterin without the adjuvant or with 0.2% iCGN did not confer significant protection. Chickens immunized by the i.c./i.b. routes with the adjuvanted bacterin had increased levels of circulating and local anti-MG IgG but not IgM or IgA. Tracheal populations of MG were reduced when compared with unimmunized controls.
authors.affiliation: Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602-4875.
authors.forename: M G
authors.initials: MG
authors.lastname: Elfaki
authors.forename: S H
authors.initials: SH
authors.lastname: Kleven
authors.forename: L H
authors.initials: LH
authors.lastname: Jin
authors.forename: W L
authors.initials: WL
authors.lastname: Ragland
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000032:analysis; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D002351:Carrageenan / Q000008:administration & dosage*; D002645:Chickens; D007114:Immunization / Q000379:methods*; D007136:Immunoglobulins / Q000096:biosynthesis; D007267:Injections; D007274:Injections, Intraperitoneal; D008221:Lymphoid Tissue; D008297:Male; D009174:Mycoplasma / Q000276:immunology* / Q000302:isolation & purification; D013997:Time Factors; D015164:Vaccines, Inactivated / Q000008:administration & dosage
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007136:Immunoglobulins; D015164:Vaccines, Inactivated; D002351:Carrageenan