title: Bioavailability to rats of bound [14C] pirimiphos-methyl in stored wheat.
abstract: Stored wheat treated with radiolabelled pirimiphos-methyl (0-2-diethyl-amino-6-methyl-pyrimidin-4-yl 0,0-dimethyl phosphorothioate) formed bound (nonextractable) 14C residues. Supercritical fluid extraction, gas chromatography and mass spectrometric techniques were used to identify and quantitate the 14C bound residues in wheat grains. The amount of bound 14C residues present after 28 weeks of storage was about 9.9% of the applied radioactivity. Pirimiphos-methyl accounted for 80% of the bound residue. Grain-bound residues were fed to rats for 5 days. After a total period of 8 days a substantially large percentage of the administered bound 14C residues (72.9%) was eliminated in urine while feces contained only 17.9%. Bound pirimiphos-methyl in wheat grain was metabolized in rats by processes involving hydrolysis, N-dealkylation and 0-demethylation. The results indicate that wheat-bound residues of pirimiphos-methyl are highly bioavailable to the rat and may possess a toxicological potential as manifested by a significant reduction in body weight gain.
authors.affiliation: Centre for Land and Biological Resources Research, Agriculture Canada, Ottawa, Ontario.
authors.forename: S U
authors.initials: SU
authors.lastname: Khan
authors.forename: S
authors.initials: S
authors.lastname: Kacew
authors.forename: W
authors.initials: W
authors.lastname: Matthews
mesh_terms: D000818:Animals; D001682:Biological Availability; D001835:Body Weight / Q000187:drug effects; D002250:Carbon Radioisotopes; D005506:Food Contamination; D005511:Food Handling; D007306:Insecticides / Q000032:analysis / Q000493:pharmacokinetics* / Q000633:toxicity; D008297:Male; D009946:Organothiophosphorus Compounds / Q000032:analysis / Q000493:pharmacokinetics* / Q000633:toxicity; D010573:Pesticide Residues / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains; D014908:Triticum
chemical_list: D002250:Carbon Radioisotopes; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D010573:Pesticide Residues; C014153:pirimiphos methyl