rol A modulates the sensitivity to auxin of the proton translocation catalyzed by the plasma membrane H(+)-ATPase in transformed tobacco.
In order to investigate the effect of the rol A gene product on the plasma membrane response to auxin, a clone of rol A-transformed tobacco was prepared. Auxin sensitivity was measured by the auxin concentration which induced the highest stimulation of H(+)-ATPase-mediated proton translocation on isolated plasma membrane vesicles. Both transformed and control plants exhibited identical auxin-sensitivity changes during vegetative and induction to flowering periods. However the sensitivity of flowering-transformed plants was 100-times higher than that of control plants. Consistent observations were also made when using rol A+B+C-transformed plants. These results suggest that the rol A gene product could either participate in or affect the reception/transduction pathway of auxin signal at the plasma membrane.
Vansuyt
G
G
INRA, Physiopathologie Végétale, Dijon, France.
Vilaine
F
F
Tepfer
M
M
Rossignol
M
M
D001692:Biological Transport; D002384:Catalysis; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology*; D010947:Plants, Toxic; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012231:Rhizobium / Q000235:genetics*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic
D007210:Indoleacetic Acids; D006180:Proton-Translocating ATPases