Purification and characterization of a boar sperm protein interacting with immunoglobulins from an infertile woman.
Immunoglobulin G (IgG) fraction was prepared from a serum obtained from an infertile woman (IS) that induced sperm agglutination of human and boar sperm. The antisperm antibodies interacted with a 50-kD boar sperm protein, determined by immunoblot. The 50-kD protein was extracted with deoxycholate (DOC) and purified by affinity chromatography on concanavalin A column, ion exchange chromatography on CM52 column, affinity chromatography on IS IgG-protein G-agarose, and by preparative electrophoresis with electroelution. The purified 50-kD protein migrated as a single homogeneous band when analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and by Western blot. The production of antibodies against specific sperm proteins may be a cause of immunological infertility.
Yoshida
H
H
Center for Biomedical Research, Population Council, New York, New York 10021.
Kamada
M
M
Koide
S S
SS
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism*; D007247:Infertility, Female / Q000276:immunology*; D008297:Male; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism*; D013073:Sperm Agglutination / Q000276:immunology; D013094:Spermatozoa / Q000737:chemistry*; D013552:Swine
D000906:Antibodies; D007074:Immunoglobulin G; D011506:Proteins; D003208:Concanavalin A