Selective susceptibility of cultured striatal neurons to kainic acid.
Previous work has shown a selective sensitivity of striatal D2-receptor-containing neurons (D2 cells) to kainic acid (KA). In order to see whether this phenomenon exists in cultured cells, which could provide a very accessible model for subsequent mechanistic studies, we examined striatal cultures grown for up to 30 days and labeled with either [3H]spiperone, for D2 receptors, or with [3H]-SCH23390, for D1 receptors. Analysis of the cells was performed with a digital imaging system (RAS). The cultured cells were examined for ligand binding to receptors, cell size, and susceptibility to KA. The D2 cells showed an increased mortality over D1-receptor-containing neurons (D1 cells) in the presence of KA, of a magnitude similar to in vivo loss, with larger D2 cells showing the greatest vulnerability.
Mesco
E R
ER
Gerontology Research Center, NIA/NIH, Baltimore, Maryland.
Joseph
J A
JA
Roth
G S
GS
D000375:Aging / Q000502:physiology; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology* / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000494:pharmacology; D007608:Kainic Acid / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000187:drug effects; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2
D000596:Amino Acids; D005904:Glial Fibrillary Acidic Protein; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D007608:Kainic Acid