Identification of invasive Yersinia species using oligonucleotide probes.
Oligonucleotide probes directed to the inv and ail invasion genes of Yersinia species were used to analyse yersiniae and non-yersiniae isolates by colony hybridization. The INV-3 probe, targeted to the inv gene of Yersinia pseudotuberculosis, hybridized with all 48 HeLa cell-invasive Y. pseudotuberculosis isolates examined; the PF-13 probe, specific for the ail gene of Yersinia enterocolitica, identified all invasive strains (36 of 52) of Y. enterocolitica tested. Neither probe hybridized with non-yersinia isolates or other Yersinia species. Southern analyses of restriction enzyme-digested genomic DNA confirmed the specificity of both probes. INV-3 hybridized with a 4.5 kilobase (kb) Bam HI fragment known to carry the inv gene in Y. pseudotuberculosis. PF-13 was specific for a 1.2 kb Cla I-Ava I fragment in Y. enterocolitica that carried the ail locus. Reactivity with either probe correlated closely with the ability of Y. pseudotuberculosis and Y. enterocolitica isolates to invade HeLa cells.
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Division of Microbiology, Food and Drug Administration, Washington, DC 20204.
D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000235:genetics; D010641:Phenotype; D014774:Virulence / Q000235:genetics; D015007:Yersinia / Q000235:genetics* / Q000472:pathogenicity
D015345:Oligonucleotide Probes