Formation of beta-galactosyl compounds of pyridoxine in growing culture of Sporobolomyces singularis.
Several pyridoxine compounds were found to be formed in a high yield in a growing culture of Sporobolomyces singularis containing lactose and pyridoxine. Three compounds, I, II and III, were isolated from cultured broth by Dowex 50W-X8 column chromatography, paper chromatography, lyophilization, and then obtained as needle crystals (m.p. (decomp.): I, 204-206 degrees C; II, 192-194 degrees C; III, 222-223 degrees C. [alpha]D16: I, -27.7 degrees (c = 3.82, H2O); II, -1.6 degrees (c = 2.52, H2O); III, +8.3 degrees (c = 3.98, H2O)). Compounds I, II and III were identified as 5'-O-(beta-D-galactopyranosyl)-pyridoxine, 4'-O-(beta-D-galactopyranosyl)-pyridoxine, and 4'-O-(beta-D-galactopyranosyl-(1----4)-beta-D-galactopyranosyl)-pyrid oxi ne, respectively, on the basis of the various experimental results, viz., ultraviolet, infra-red, 1H-NMR, and 13C-NMR spectra, products by hydrolysis with acid and with alpha- and beta-galactosidases, migration on paper electrophoresis, and Gibbs reaction in the presence and absence of boric acid. Also, the yeast produced a remarkable amount of beta-glucosyl compounds of pyridoxine in cultured broth, when grown on cellobiose and pyridoxine.
Suzuki
Y
Y
Research Institute for Bioresources, Okayama University Kurashiki, Japan.
Uchida
K
K
D002240:Carbohydrate Sequence; D005285:Fermentation; D005697:Galactosides / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D003904:Mitosporic Fungi / Q000378:metabolism*; D008969:Molecular Sequence Data; D011736:Pyridoxine / Q000031:analogs & derivatives* / Q000302:isolation & purification
D005697:Galactosides; D011736:Pyridoxine