title: Assignment of the gene encoding the catalytic subunit C beta of cAMP-dependent protein kinase to the p36 band on chromosome 1.
abstract: A cDNA for the human catalytic subunit (C beta) of cAMP-dependent protein kinase (PKA) has been cloned from a testis cDNA library. In the present study, we have determined the chromosomal localization of this gene using a cDNA for C beta as a probe. Southern blot analysis of genomic DNA from human/mouse cell hybrids revealed that the presence or absence of a 20-kb XbaI fragment, which hybridized with the C beta probe, was concordant with the presence of human chromosome 1. In situ hybridization to metaphase chromosome confirmed the somatic cell hybrid data and regionally mapped the C beta gene of PKA to the p36 band on chromosome 1.
authors.affiliation: MRC Group in Molecular Endocrinology, CHUL Research Center and Laval University, Québec, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Simard
authors.forename: D
authors.initials: D
authors.lastname: Bérubé
authors.forename: M
authors.initials: M
authors.lastname: Sandberg
authors.forename: K H
authors.initials: KH
authors.lastname: Grzeschik
authors.forename: R
authors.initials: R
authors.lastname: Gagné
authors.forename: V
authors.initials: V
authors.lastname: Hansson
authors.forename: T
authors.initials: T
authors.lastname: Jahnsen
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D011494:Protein Kinases / Q000235:genetics*
chemical_list: D015342:DNA Probes; D011494:Protein Kinases