title: Mapping the whole human genome by fingerprinting yeast artificial chromosomes.
abstract: Physical mapping of the human genome has until now been envisioned through single chromosome strategies. We demonstrate that by using large insert yeast artificial chromosomes (YACs) a whole genome approach becomes feasible. YACs (22,000) of 810 kb mean size (5 genome equivalents) have been fingerprinted to obtain individual patterns of restriction fragments detected by a LINE-1 (L1) probe. More than 1000 contigs were assembled. Ten randomly chosen contigs were validated by metaphase chromosome fluorescence in situ hybridization, as well as by analyzing the inter-Alu PCR patterns of their constituent YACs. We estimate that 15% to 20% of the human genome, mainly the L1-rich regions, is already covered with contigs larger than 3 Mb.
authors.affiliation: Centre d'Etude du Polymorphisme Humain, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Bellanné-Chantelot
authors.forename: B
authors.initials: B
authors.lastname: Lacroix
authors.forename: P
authors.initials: P
authors.lastname: Ougen
authors.forename: A
authors.initials: A
authors.lastname: Billault
authors.forename: S
authors.initials: S
authors.lastname: Beaufils
authors.forename: S
authors.initials: S
authors.lastname: Bertrand
authors.forename: I
authors.initials: I
authors.lastname: Georges
authors.forename: F
authors.initials: F
authors.lastname: Glibert
authors.forename: I
authors.initials: I
authors.lastname: Gros
authors.forename: G
authors.initials: G
authors.lastname: Lucotte
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal; D015342:DNA Probes; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
chemical_list: D015342:DNA Probes