A 1.5 kb sequence homology in 3'-terminal regions of RNA-1 and RNA-2 of a birch isolate of cherry leaf roll nepovirus is also present, in part, in a rhubarb isolate.
A series of cDNA clones has been made from the birch isolate of cherry leaf roll nepovirus. Restriction enzyme analysis and sequencing showed that at the 3' end, RNA-1 and RNA-2 are identical for 1.5 kb. Also a 0.7 kb 3' end homology exists between the birch and rhubarb isolate. These sequences do not seem to code for any proteins; however, the sequence conservation points to a role in virus replication.
Scott
N W
NW
NERC Institute of Virology and Environmental Microbiology, Oxford, U.K.
Cooper
J I
JI
Liu
Y Y
YY
Hellen
C U
CU
D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D010942:Plant Viruses / Q000235:genetics*; D010946:Plants, Medicinal; D012367:RNA, Viral / Q000737:chemistry*; D015183:Restriction Mapping; D012250:Rheum / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D014197:Trees / Q000382:microbiology*; D014779:Virus Replication / Q000235:genetics
D004279:DNA, Viral; D012367:RNA, Viral