title: Evidence for a novel splice variant of the alpha subunit of Go in rat male haploid germ cells.
abstract: Northern analysis shows that Go alpha mRNA is highly expressed in immature germ cells from rat. Whereas alpha o2 mRNA is the major form in pachytene spermatocytes, a message of shorter chain length is present in large amount in haploid germ cells. This mRNA was detected with an oligonucleotide specific for the 3'-coding region of alpha o2, but did not hybridize to oligonucleotides specific for the 5'-untranslated and 5'-coding regions. The results indicate the presence of a novel splice variant of alpha o mRNA, which may code for a Go alpha protein important for germ cell development.
authors.affiliation: Institute of Medical Biochemistry, University of Oslo, Norway.
authors.forename: T B
authors.initials: TB
authors.lastname: Haugen
authors.forename: W
authors.initials: W
authors.lastname: Eskild
authors.forename: V
authors.initials: V
authors.lastname: Hansson
mesh_terms: D000818:Animals; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D019204:GTP-Binding Proteins / Q000235:genetics*; D014644:Genetic Variation; D006238:Haploidy; D008297:Male; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D012708:Sertoli Cells / Q000737:chemistry*; D013094:Spermatozoa / Q000737:chemistry*
chemical_list: D012333:RNA, Messenger; D019204:GTP-Binding Proteins; D019206:GTP-Binding Protein alpha Subunits, Gi-Go