Is ribosomal cistron activity increased in human lymphoblastoid cell lines?
The genomic activity of nucleolar organizer regions (NORs) in eight human B-cell lymphoblastoid cell lines was studied following the routinely used Ag-NOR technique. The results demonstrate that (a) Ag-NORs are located in the short arms of D- and G-group chromosomes, (b) two out of eight cell lines have 66.7% and 49.0% of metaphases, respectively, with 9 to 10 active Ag-NORs, and (c) as a whole, Ag-NOR activity is much higher in B-cell lines as compared with conventional 72-hr peripheral blood lymphocyte (T-cell) harvests.
Gupta
N J
NJ
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Hopwood
V L
VL
Pathak
S
S
D001402:B-Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000648:ultrastructure; D004275:DNA, Ribosomal / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D009697:Nucleolus Organizer Region / Q000378:metabolism / Q000648:ultrastructure*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D016622:Silver Staining
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal