Cloning and expression of an inducible lymphoid-specific, protein tyrosine phosphatase (HePTPase).
Tyrosine phosphorylation and dephosphorylating events have been shown to be central to the process of growth regulation and signal transduction. We report here, the identification of a new gene with a tyrosine phosphatase domain (EC 3.1.3.48) which is expressed exclusively in thymus and spleen. A cDNA of 2760 bp encodes a 339-amino acid, intracellular, single-domain tyrosine phosphatase. When expressed as a glutathionine-S-transferase fusion protein, efficient lysis of p-nitrophenyl phosphate is noted, indicating in vitro enzymatic activity of the cloned gene product. Normal mouse lymphocytes increase mRNA expression 10-15-fold upon stimulation with phytohemagglutinin, concanavalin A, lipopolysaccharide or anti-CD3 monoclonal antibody. This new hematopoietic tyrosine phosphatase, (HePTP), may play a role in the regulation of T and B lymphocyte development and signal transduction.
Zanke
B
B
Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Canada.
Suzuki
H
H
Kishihara
K
K
Mizzen
L
L
Minden
M
M
Pawson
A
A
Mak
T W
TW
D000595:Amino Acid Sequence; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D001483:Base Sequence; D017252:CD3 Complex; D003001:Cloning, Molecular; D003208:Concanavalin A; D008070:Lipopolysaccharides; D008214:Lymphocytes / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins; D017027:Protein Tyrosine Phosphatases / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D011948:Receptors, Antigen, T-Cell / Q000502:physiology
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D003208:Concanavalin A; D017027:Protein Tyrosine Phosphatases