title: Altered pterin patterns in photobehavioral mutants of Phycomyces blakesleeanus.
abstract: Pterins were extracted with methanol from sporangiophores of the lower fungus Phycomyces blakesleeanus and separated and identified by high performance liquid chromatography (HPLC) with fluorescence detection. The following pterins were found and identified for the wild-type strain NRRL1555: carboxypterin (6.7 x 10(-6) M), neopterin (4.2 x 10(-7) M), xanthopterin (5.3 x 10(-6) M), biopterin (3.9 x 10(-7) M), pterin (9.1 x 10(-7) M), and 6,7-dimethylpterin (1.2 x 10(-6) M). The HPLC elution profiles of the wild type were compared to a set of phototropism mutants (genotype mad) with specific defects in the light-transduction pathway. The mutant profiles were qualitatively similar to those of the wild type. Quantitative differences were, however, discerned for madA, madC, and madH mutants. The madA mutation was associated with increased amounts of biopterin and 6,7-dimethylpterin and a reduction of neopterin, pterin, xanthopterin, and unidentified pterins eluting at 14-18 min. The stimulatory effect of the madA mutation on biopterin and 6,7-dimethylpterin appears to be compensated by a secondary mutation (pde) which is responsible for the loss of 75% of adenosine 3',5'-cyclic monophosphate (cAMP)-phosphodiesterase activity. In a madA pde double mutant the amounts of biopterin and 6,7-dimethylpterin fell below the wild-type level. These results suggest that an increased level of endogenous cAMP represses the biosynthesis of these pterins. The madC mutation increased the amounts of biopterin and xanthopterin and that of the unidentified pterins which could be derivatized to carboxypterin. Single madB mutations had, compared to the wild type, two times higher amounts of biopterin and two times lower amounts of neopterin.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Fachbereich Biologie/Botanik der Philipps-Universität Marburg, Fed. Rep. Germany.
authors.forename: N
authors.initials: N
authors.lastname: Hohl
authors.forename: P
authors.initials: P
authors.lastname: Galland
authors.forename: H
authors.initials: H
authors.lastname: Senger
mesh_terms: D005415:Flavins / Q000378:metabolism; D005800:Genes, Fungal; D009154:Mutation; D010777:Photochemistry; D010786:Photoreceptor Cells / Q000378:metabolism; D010800:Phycomyces / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D011622:Pterins / Q000378:metabolism*
chemical_list: D005415:Flavins; D011622:Pterins