title: [Diastereomers of nonionic oligonucleotide analogs. VI. Isolation of diastereomers of ethyl phosphotriesters of the octanucleotide d(GCCAAACA) by high performance affinity chromatography].
abstract: Diastereomers of oligonucleotide ethyl phosphotriesters were separated by high-performance complementary (affinity) chromatography on a column with the immobilized complementary oligonucleotide. The elution buffer contained 0.18 M K2HPO4, pH 7.5, and 30% acetonitrile. The temperature of the separation was a few degrees lower than Tm of corresponding oligonucleotide complexes. The diastereomers separated completely or partially were: d[GCC(Et)AAACA], d[GCCA(Et)AACA], d[GCAA(Et)ACA], d[GCC(Et)A(Et)AACA], d[GCC(Et)AA(Et)ACA], d[GCCA(Et)A(Et)ACA], d[GCC(Et)A(Et)A(Et)ACA].
authors.forename: I A
authors.initials: IA
authors.lastname: Vtorushina
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Gorbunov
authors.forename: V F
authors.initials: VF
authors.lastname: Zarytova
authors.forename: N I
authors.initials: NI
authors.lastname: Komarova
authors.forename: A V
authors.initials: AV
authors.lastname: Lebedev
authors.forename: S G
authors.initials: SG
authors.lastname: Lokhov
authors.forename: A V
authors.initials: AV
authors.lastname: Manaenko
authors.forename: A N
authors.initials: AN
authors.lastname: Siniakov
authors.forename: I G
authors.initials: IG
authors.lastname: Shishkina
mesh_terms: D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D009841:Oligonucleotides; D009943:Organophosphorus Compounds
vernacular_title: Issedovanie diastereomerov neionnykh analogov oligonukleotidov. VI. Razdelenie diastereomerov étilovykh fosfotriéfirov oktanukleotida d(GCCAAACA) metodom vysokoéffektivnoĭ affinnoĭ khromatografii.