Structure and expression of the barley lipid transfer protein gene Ltp1.
We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of Ltp1 showed that it contains an open reading frame of 351 bp interrupted by a single intron of 133 bp. Transient expression assays indicated that 702 bp of the 5' upstream region of Ltp1 is sufficient to direct aleurone-specific expression during late seed development and early germination.
Skriver
K
K
Carlsberg Research Laboratory, Copenhagen, Denmark.
Leah
R
R
Müller-Uri
F
F
Olsen
F L
FL
Mundy
J
J
D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D017343:Genes, Plant; D001467:Hordeum / Q000235:genetics* / Q000378:metabolism; D007438:Introns; D007700:Kinetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D014158:Transcription, Genetic
D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C511920:lipid transfer proteins, plant