Effects of anti-triadin antibody on Ca2+ release from sarcoplasmic reticulum.
The monoclonal antibody, mAb GE 4.90, raised against triadin, a 95 kDa protein of sarcoplasmic reticulum (SR), inhibits the slow phase of Ca2+ release from SR following depolarization of the T-tubule moiety of the triad. The antibody has virtually no effect on the fast phase of depolarization-induced Ca2+ release nor on caffeine-induced Ca2+ release. Since the slow phase of depolarization-induced Ca2+ release is also inhibited by dihydropyridines (DHP), these results suggest that triadin may be involved in the functional coupling between the DHP receptor and the SR Ca2+ channel.
Brandt
N R
NR
Department of Pharmacology, University of Miami School of Medicine, FL 33101.
Caswell
A H
AH
Brunschwig
J P
JP
Kang
J J
JJ
Antoniu
B
B
Ikemoto
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins; D007700:Kinetics; D009124:Muscle Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D009124:Muscle Proteins; C070048:triadin; D002118:Calcium