Microwave irradiation and cross-linking of collagen.
In a multifactorial experiment, dermal sheep collagen was treated in diluted glutaraldehyde solutions, 70% ethyl alcohol, Cialit 1:5000, and distilled water for 1, 3 and 5 min, respectively, in combination with microwave irradiation at different temperature settings. The shrinkage temperature indicating the degree of cross-linking achieved was then determined. Treatment in 0.65% glutaraldehyde with microwave irradiation setting for 60 degrees C resulted in the maximum shrinkage temperature within 1 min, whilst at the lower setting of 50 degrees C, the maximum shrinkage temperature for both glutaraldehyde solutions is only reached after 5 min. Neither microwave irradiation by itself, Cialit or ethyl alcohol induce cross-linking of collagen fibres. These findings are relevant for implant studies.
Visser
C E
CE
Department of Otorhinolaryngology, Academic Medical Centre, Amsterdam, The Netherlands.
Voute
A B
AB
Oosting
J
J
Boon
M E
ME
Kok
L P
LP
D000818:Animals; D001672:Biocompatible Materials; D003094:Collagen / Q000737:chemistry / Q000528:radiation effects*; D003432:Cross-Linking Reagents; D005976:Glutaral; D066298:In Vitro Techniques; D008422:Materials Testing; D008872:Microwaves
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D003094:Collagen; D005976:Glutaral