Hexamethylene bisacetamide and cis-platin stimulate the expression from the HIV-1 long terminal repeat sequence in human MCF-7 cells.
We have employed a recombinant plasmid pBHIV-1 carrying the Long Terminal Repeat sequences (LTR) of the Human Immunodeficiency Virus (HIV-1) linked to the reporter chloramphenicol acetyl transferase (CAT) gene and to the aminoglycoside phosphotransferase (aph) gene as a selectable marker. We have introduced pBHIV-1 DNA in human breast cancer MCF-7 cells and obtained the MCF-7HIV-1 cell line, resistant to geneticin. We have studied the effect of hexamethylene-bisacetamide (HMBA), cis-platin, interferon-aB, dexamethasone and tamoxifen on the LTR regulated CAT activity in MCF-7HIV-1 cells. It was found that HMBA and cis-platin stimulated CAT activity, whereas interferon-aB, dexamethasone and tamoxifen had no significant effect.
Tsatsanis
C
C
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
Tsiriyotis
C
C
Spandidos
D A
DA
D000081:Acetamides / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology; D002945:Cisplatin / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D016325:HIV Long Terminal Repeat / Q000187:drug effects*; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D016898:Interferon-alpha; D009376:Neoplasms, Hormone-Dependent / Q000473:pathology; D011994:Recombinant Proteins; D013268:Stimulation, Chemical; D013629:Tamoxifen / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000081:Acetamides; D007370:Interferon Type I; D016898:Interferon-alpha; D011994:Recombinant Proteins; C076799:interferon-alpha 8; D013629:Tamoxifen; D003907:Dexamethasone; C014026:hexamethylene bisacetamide; D002945:Cisplatin