Incomplete splicing of simian virus 40 large T antigen transcripts in a transformed mouse cell line.
The SV40 large T antigen transcripts from an SV40-transformed mouse embryo fibroblast cell line (215) have been analyzed by Northern blots and by mapping and sequencing of the corresponding cDNAs. We have observed that T antigen mRNA is highly overexpressed in the 215 cells, and is mostly unspliced, but there are no sequence changes at the splice sites. However, normal amounts of wild-type T antigen are produced, suggesting that the splicing and translation of T antigen RNA is tightly controlled in these cells.
Kennady
P K
PK
Centre for Cellular and Molecular Biology, Hyderabad, India.
Parnaik
V K
VK
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D051379:Mice; D011233:Precipitin Tests; D012326:RNA Splicing; D015183:Restriction Mapping; D014158:Transcription, Genetic
D000952:Antigens, Polyomavirus Transforming