Glibenclamide inhibits a voltage-gated K+ current in the human neuroblastoma cell line SH-SY5Y.
The whole-cell patch-clamp technique was used to record outward K+ currents in the human neuroblastoma cell line SH-SY5Y. These K+ currents were inhibited by tetraethylammonium (20 mM) and by 4-aminopyridine (2 mM). The Ca2+ channel blocker Cd2+ (0.2 mM) also inhibited K+ currents, indicating that a component was Ca(2+)-activated. Glibenclamide, a presumed selective inhibitor of ATP-sensitive K+ channels, caused reversible inhibitions of the K+ currents and appeared to accelerate their inactivation. These effects were not significantly affected by intracellular ATP (ATPi), and were observed in the presence of 0.2 mM Cd2+. It is concluded that glibenclamide inhibits a voltage-gated, Ca(2+)- and ATPi-independent K+ current in SH-SY5Y cells.
Reeve
H L
HL
Department of Pharmacology, University of Leeds, U.K.
Vaughan
P F
PF
Peers
C
C
D015761:4-Aminopyridine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology; D002460:Cell Line; D005905:Glyburide / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D009447:Neuroblastoma; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D015221:Potassium Channels; D013757:Tetraethylammonium Compounds; D002104:Cadmium; D019789:Tetraethylammonium; D000255:Adenosine Triphosphate; D015761:4-Aminopyridine; D005905:Glyburide