In vitro biosynthesis of complement factor I by human endothelial cells.
We have studied the secretion of the complement regulatory protein factor I by human umbilical vein endothelial cells (HUVEC). Northern and Western blot analysis and biosynthetic labeling experiments indicate that HUVEC secrete factor I at very low levels in basal conditions and that this secretion is significantly enhanced by interferon-gamma. Analysis of the proteolytic inactivation of C3b by HUVEC supernatants show that factor I is secreted in a functional form and can promote the specific proteolytic inactivation of C3b to iC3b. Together with previous studies establishing the secretion of complement factor H by HUVEC, this work demonstrates that the endothelial cell is able to secrete in its environment two complement regulatory proteins, factor I and factor H, which can mediate the degradation of C3b to iC3b. The secretion of factor I by HUVEC provides a useful in vitro model to analyze the modulation of this secretion and may be relevant to the local deposition of iC3b at the surface of the endothelium during the inflammatory reaction.
Julen
N
N
INSERM Unité 78, Bois-Guillaume.
Dauchel
H
H
Lemercier
C
C
Sim
R B
RB
Fontaine
M
M
Ripoche
J
J
D002478:Cells, Cultured; D003179:Complement C3b / Q000378:metabolism; D003180:Complement C3b Inactivator Proteins / Q000096:biosynthesis*; D017244:Complement Factor I; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D012697:Serine Endopeptidases / Q000096:biosynthesis* / Q000235:genetics
D003180:Complement C3b Inactivator Proteins; D007375:Interleukin-1; D012333:RNA, Messenger; D003179:Complement C3b; D007371:Interferon-gamma; D012697:Serine Endopeptidases; D017244:Complement Factor I