Functional molecular mass of the 14C-azidobenzamidotaurocholic acid binding proteins in hepatocellular bile acid transport systems.
The apparent target size of 14C-azidobenzamidotaurocholate binding proteins in basolateral rat liver plasma membranes (blPm) was determined by analysis of the radiation induced decrease of the binding of this photoreactive taurocholate analog to blPm. Radiation causes a dose-dependent mono-exponential reduction of binding of ABATC to the protein subunits with molecular masses of 48-50 and 52-54 kDa in SDS-PAGE. The minimal functional molecular mass of the 48-50 and 52-54 kDa ABATC binding proteins was determined to be 99 +/- 8.2 and 93.2 +/- 7 kDa, respectively.
Ziegler
K
K
Institute of Pharmacology and Toxicology, Justus-Liebig University, Giessen, Germany.
Elsner
R H
RH
D000818:Animals; D001386:Azides / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008970:Molecular Weight; D010777:Photochemistry; D051381:Rats; D013656:Taurocholic Acid / Q000031:analogs & derivatives* / Q000378:metabolism
D001386:Azides; D001647:Bile Acids and Salts; D002352:Carrier Proteins; C059201:azidobenzamidotaurocholate; D013656:Taurocholic Acid