Isolation and characterization of additional genes influencing resistance to various mutagens in the yeast Saccharomyces cerevisiae.
Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNNG, to 4-NQO and triaziquone, and to mutagens 4-NQO, MNNG, and triaziquone, respectively. Integration of the gene disruption-constructs into the haploid yeast genome yielded viable null-mutants with a mutagen-sensitive phenotype. Thus, copy number of these non-essential yeast genes determines the relative resistance to certain chemical mutagens, with zero copies yielding a phenotype of mutagen sensitivity and multiple copies one of mutagen hyper-resistance, respectively.
Haase
E
E
Institut für Mikrobiologie der J.W. Goethe-Universität, Frankfurt/Main, Federal Republic of Germany.
Servos
J
J
Brendel
M
M
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D003001:Cloning, Molecular; D004260:DNA Repair / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens / Q000378:metabolism / Q000494:pharmacology*; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014228:Triaziquone / Q000494:pharmacology
D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D015112:4-Nitroquinoline-1-oxide; D014228:Triaziquone