Differential induction responses of delta-aminolevulinate synthase mRNAs during erythroid differentiation: use of nonradioactive in situ hybridization.
Expression of mRNAs encoding the erythroid-specific delta-aminolevulinate synthase (ALAS-E) and the nonspecific delta-aminolevulinate synthase (ALAS-N) were examined in murine Friend virus-transformed erythroleukemia (MEL) cells using nonradioactive in situ hybridization. Following dimethyl sulfoxide (DMSO) treatment, ALAS-E mRNA increased markedly, while ALAS-N mRNA did not increase in wild-type MEL cells. In contrast, in a DMSO-resistant clone of MEL cells, ALAS-E was not detectable before and after DMSO treatment. These findings suggest that ALAS-E and ALAS-N mRNAs are under separate controls and that the expression of ALAS-E mRNA is a critical event in erythroid differentiation.
Mitani
K
K
Rockefeller University, New York, NY 10021.
Fujita
H
H
Hayashi
N
N
Yamamoto
M
M
Sassa
S
S
D000624:5-Aminolevulinate Synthetase / Q000235:genetics*; D000818:Animals; D004273:DNA, Neoplasm / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015672:Erythroid Precursor Cells / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D004920:Erythropoiesis / Q000235:genetics* / Q000502:physiology; D005622:Friend murine leukemia virus; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D007527:Isoenzymes / Q000235:genetics; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D051379:Mice; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology / Q000473:pathology
D004273:DNA, Neoplasm; D007527:Isoenzymes; D012333:RNA, Messenger; D000624:5-Aminolevulinate Synthetase; D004121:Dimethyl Sulfoxide