title: Induction of severe vascular leakage by low doses of Escherichia coli hemolysin in perfused rabbit lungs.
abstract: S. aureus alpha-toxin and E. coli hemolysin (Hly) represent two prototypes of pore-forming cytolysins. Both are established virulence factors and have been implicated in the development of septic lung failure. Low doses of these agents cause thromboxane-mediated vasoconstriction and edema formation in isolated perfused rabbit lungs. In a preceding investigation, we observed that alpha-toxin causes overt endothelial cell damage in these lungs, as demonstrable by electron microscopy (Seeger W, Birkemeyer RG, Ermert L, Suttorp N, Bhakdi S, Duncker HR: Lab Invest 63:341, 1990). Here, we present results of a parallel study conducted with E. coli hemolysin. Thromboxane-dependent pulmonary hypertension was suppressed by the addition of acetylsalicylic acid to the perfusion fluid in all cases. Administration of 0.2 hemolytic units (HU; i.e., 20 ng/ml protein) resulted in progressive weight gain after a lag period of 10 to 15 minutes, and 30 minutes after toxin application the gravimetrically determined capillary filtration coefficients (Kfc) were increased greater than 10-fold. Perfusion was terminated when the total lung weight gain surpassed 20 gm. 0.12 HU/ml E. coli hemolysin caused 2- to 3-fold increased capillary filtration coefficients values within 110 minutes, concomitant with intermediate quantities of edema formation (9.7 +/- 2.7 gm). Potassium liberation in the absence of lactate dehydrogenase release occurred in all toxin treated lungs. Electron microscopic examination after perfusion fixation revealed interstitial edema formation in areas remote from the blood-gas exchange barrier. Increased numbers of endothelial plasmalemmal vesicles were visualized at the very onset of edema formation in lungs exposed to 0.2 HU/ml, and after a 110-minute exposure to 0.12 HU/ml of the toxin, but not in lungs exhibiting severe edema (greater than 20 gm weight gain). In contrast to our previous results with alpha-toxin, endothelial cells displayed normal electron density here and were not detached from the fused basal lamina. Hence, although both pore formers provoke severe vascular leakage in our experimental model, the underlying pathways probably divert fundamentally from each other.
authors.affiliation: Department of Internal Medicine, Justus-Liebig-University Giessen, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Ermert
authors.forename: S
authors.initials: S
authors.lastname: Rousseau
authors.forename: H
authors.initials: H
authors.lastname: Schütte
authors.forename: R G
authors.initials: RG
authors.lastname: Birkemeyer
authors.forename: F
authors.initials: F
authors.lastname: Grimminger
authors.forename: S
authors.initials: S
authors.lastname: Bhakdi
authors.forename: H R
authors.initials: HR
authors.lastname: Duncker
authors.forename: W
authors.initials: W
authors.lastname: Seeger
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000633:toxicity*; D002199:Capillary Permeability / Q000187:drug effects; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005260:Female; D006460:Hemolysin Proteins / Q000008:administration & dosage / Q000633:toxicity*; D066298:In Vitro Techniques; D008168:Lung / Q000098:blood supply* / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D010477:Perfusion; D011652:Pulmonary Circulation / Q000187:drug effects; D011654:Pulmonary Edema / Q000139:chemically induced* / Q000473:pathology; D011817:Rabbits
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli