A novel mitogen-inducible gene product related to p50/p105-NF-kappa B participates in transactivation through a kappa B site.
A Rel-related, mitogen-inducible, kappa B-binding protein has been cloned as an immediate-early activation gene of human peripheral blood T cells. The cDNA has an open reading frame of 900 amino acids capable of encoding a 97-kDa protein. This protein is most similar to the 105-kDa precursor polypeptide of p50-NF-kappa B. Like the 105-kDa precursor, it contains an amino-terminal Rel-related domain of about 300 amino acids and a carboxy-terminal domain containing six full cell cycle or ankyrin repeats. In vitro-translated proteins, truncated downstream of the Rel domain and excluding the repeats, bind kappa B sites. We refer to the kappa B-binding, truncated protein as p50B by analogy with p50-NF-kappa B and to the full-length protein as p97. p50B is able to form heteromeric kappa B-binding complexes with RelB, as well as with p65 and p50, the two subunits of NF-kappa B. Transient-transfection experiments in embryonal carcinoma cells demonstrate a functional cooperation between p50B and RelB or p65 in transactivation of a reporter plasmid dependent on a kappa B site. The data imply the existence of a complex family of NF-kappa B-like transcription factors.
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Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Burd
P R
PR
Brown
K
K
Villalobos
J
J
Park
S
S
Ryseck
R P
RP
Bravo
R
R
Kelly
K
K
Siebenlist
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D000595:Amino Acid Sequence; D017487:Ankyrins; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D020572:Oncogene Proteins v-rel; D016366:Open Reading Frames / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics; D011233:Precipitin Tests; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D013601:T-Lymphocytes / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*; D014407:Tumor Cells, Cultured
D017487:Ankyrins; D001798:Blood Proteins; D008565:Membrane Proteins; D008934:Mitogens; D016328:NF-kappa B; D020572:Oncogene Proteins v-rel; D010750:Phosphoproteins; D011498:Protein Precursors; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; C072386:p50B-p97 protein, human; D011505:Protein-Tyrosine Kinases