Cloning and sequencing of a COUP transcription factor gene expressed in Xenopus embryos.
A cDNA clone encoding COUP transcription factor, a member of the steroid/thyroid receptor superfamily, has been isolated from a Xenopus neurula (stage 17 embryo) library. Sequencing of this clone reveals an open reading frame encoding a 397 amino acid protein. The amino acid sequence of Xenopus COUP has been compared with its human and Drosophila homologues showing that there are few similarities within the amino-terminal region, whereas the remainder of the protein, including the putative DNA and ligand binding domains, is very well conserved.
Matharu
P J
PJ
Department of Biochemistry, University of Wales College of Cardiff, UK.
Sweeney
G E
GE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051838:COUP Transcription Factor I; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004625:Embryo, Nonmammalian; D015870:Gene Expression; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics*; D014981:Xenopus
D051838:COUP Transcription Factor I; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D004247:DNA