title: Studies of dihydroxyacetone phosphate acyltransferase in rat small intestine. Subcellular localization and effect of partially hydrogenated fish oil and clofibrate.
abstract: The subcellular localization of dihydroxyacetone phosphate acyltransferase (DHAPAT) activity in rat small intestine was investigated by Nycodenz-gradient centrifugation. We found that DHAPAT had a predominant peroxisomal distribution, with a separate enzyme activity located in the microsomal fraction, the same distribution as found in rat liver. The effect of feeding rats on a diet with 20% (w/w) partially hydrogenated fish oil (PHFO) or 0.3% clofibrate on the activity of DHAPAT in rat small intestine and liver was studied. Both 20% PHFO and 0.3% clofibrate gave a 1.8-fold stimulation of the specific activities of DHAPAT in peroxisomes of the small intestine, whereas in the liver 20% PHFO gave a 1.4-fold stimulation and 0.3% clofibrate a 1.6-fold stimulation of the total DHAPAT activities in the postnuclear supernatant. The specific activities of DHAPAT in liver were not affected.
authors.affiliation: Institute for Nutrition Research, University of Oslo, Norway.
authors.forename: B
authors.initials: B
authors.lastname: Ruyter
authors.forename: J S
authors.initials: JS
authors.lastname: Lund
authors.forename: M S
authors.initials: MS
authors.lastname: Thomassen
authors.forename: E N
authors.initials: EN
authors.lastname: Christiansen
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002994:Clofibrate / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005395:Fish Oils / Q000494:pharmacology*; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
chemical_list: D005227:Fatty Acids; D005395:Fish Oils; D000217:Acyltransferases; C020051:glycerone-phosphate O-acyltransferase; D002994:Clofibrate