Carnitine administration to juvenile visceral steatosis mice corrects the suppressed expression of urea cycle enzymes by normalizing their transcription.
Previous studies in our laboratories have revealed that juvenile visceral steatosis mice show suppressed transcription of urea cycle enzyme genes during development and are systemically deficient in carnitine. It has not yet been explained, however, how this carnitine deficiency relates to the abnormal gene expression. We investigated the effect of carnitine on abnormal gene expression, growth retardation, and fatty liver. Carnitine administration relieved the suppression of the developmental induction of two urea cycle enzymes examined, carbamoyl-phosphate synthetase and argininosuccinate synthase, and kept the activities of enzymes normal. However, carnitine did not reduce accumulated lipid in the liver to the normal level. These results suggest that carnitine deficiency plays an important role in the abnormal expression of urea cycle enzyme genes and that the abnormal expression of the genes is not directly caused by lipid accumulation in the liver.
Horiuchi
M
M
Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan.
Kobayashi
K
K
Tomomura
M
M
Kuwajima
M
M
Imamura
Y
Y
Koizumi
T
T
Nikaido
H
H
Hayakawa
J
J
Saheki
T
T
D000375:Aging; D000818:Animals; D001124:Argininosuccinate Synthase / Q000096:biosynthesis / Q000235:genetics*; D015152:Blotting, Northern; D001835:Body Weight / Q000187:drug effects; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000096:biosynthesis / Q000235:genetics*; D002331:Carnitine / Q000494:pharmacology*; D004794:Enzyme Repression / Q000187:drug effects; D005234:Fatty Liver / Q000201:enzymology* / Q000235:genetics; D005634:Fructose-Bisphosphate Aldolase / Q000096:biosynthesis / Q000235:genetics*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008817:Mice, Mutant Strains; D009954:Ornithine Carbamoyltransferase / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*; D014508:Urea / Q000378:metabolism*
D012333:RNA, Messenger; D014508:Urea; D009954:Ornithine Carbamoyltransferase; D005634:Fructose-Bisphosphate Aldolase; D002222:Carbamoyl-Phosphate Synthase (Ammonia); D001124:Argininosuccinate Synthase; D002331:Carnitine