Insoluble DNA-like phosphorylated polystyrene: specific interactions with anti-DNA antibodies from systemic lupus erythematosus patients.
Systemic lupus erythematosus (SLE) is an autoimmune disease. Antibodies directed mainly against DNA and/or phospholipids are present in the serum of SLE patients. Therefore phosphorylated polystyrene derivatives acting as DNA-like polymers should be able to interact with the SLE anti-DNA antibodies. Such functional polymers were synthesized and subsequently their interactions with the anti-DNA antibodies studied. Adsorption experiments performed with both anti-DNA antibodies and normal immunoglobulins showed high affinity constants of the phosphorylated polymer for anti-DNA antibodies (4 x 10(9) M-1) whereas for normal IgG the affinity was low (2 x 10(5) M-1). Moreover, the interaction was specific involving the idiotypic moiety of the anti-DNA antibodies and an array of phosphoester groups at the surface of this biomaterial.
Letourneur
D
D
Laboratoire de Recherches sur les Macromolécules, CSP Université Paris-Nord, Villetaneuse, France.
Jozefowicz
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M
D000327:Adsorption; D000974:Antibodies, Antinuclear; D000915:Antibody Affinity; D000941:Antigens; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D004247:DNA / Q000276:immunology; D006801:Humans; D007120:Immunochemistry; D007074:Immunoglobulin G; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008422:Materials Testing; D010766:Phosphorylation; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry
D000974:Antibodies, Antinuclear; D000941:Antigens; D001672:Biocompatible Materials; D007074:Immunoglobulin G; D011137:Polystyrenes; D004247:DNA