title: Effects of blood sampling, anesthesia and surgery on plasma vasopressin concentration in rats.
abstract: The influence of blood sampling, anesthesia and surgery on plasma vasopressin concentration was assessed in rats. Mean plasma concentration in conscious, chronically catheterized rats was 1.4 +/- 0.1 pg/ml (n = 6). This value remained constant over repeated plasma samplings in the same animals. On the other hand, decapitation increased the plasma vasopressin concentration to 6.0 +/- 2.4 (in pg/ml) (n = 6), inactin anesthesia to 2.9 +/- 0.6 (n = 6), anesthesia and femoral cannulation to 13.3 +/- 5.8 (n = 6) and surgery for renal micropuncture to 81.3 +/- 35.0 (n = 6). It is concluded that the level of circulating plasma vasopressin is highly dependent on the sampling technique and is closely related to the extent of surgery.
authors.affiliation: Département de Biologie Cellulaire et Moléculaire, CEN Saclay, Gif-sur-Yvette, France.
authors.forename: B
authors.initials: B
authors.lastname: Corman
authors.forename: G
authors.initials: G
authors.lastname: Geelen
mesh_terms: D000758:Anesthesia; D000818:Animals; D001800:Blood Specimen Collection; D001815:Bloodletting; D005260:Female; D007668:Kidney / Q000601:surgery*; D051381:Rats; D013874:Thiopental / Q000031:analogs & derivatives / Q000494:pharmacology; D014667:Vasopressins / Q000097:blood*
chemical_list: D014667:Vasopressins; C004316:thiobutabarbital; D013874:Thiopental