Production of antibodies specific for double stranded antigen DNA cloned from immune complexes in plasma of a SLE patient.
The antigenicity of antigen DNA isolated from immune complexes in plasma of a SLE patient was examined. DDY mice were immunized with the cloned antigen DNA carrying a sequence homologous with a part of bacteriophage f1 (KS8 DNA) by the coupling method, and the antibody response was estimated by radioimmunoassay. Antibodies specific to double stranded DNA were elicited. Moreover, the antibodies showed preferential binding to KS8 DNA than other DNA derived from Escherichia coli. These results suggest that KS8 DNA has a significant antigenicity in mice.
Terada
K
K
Department of Biochemistry, Akita University School of Medicine, Japan.
Hirose
S
S
Okuhara
E
E
D000910:Antibodies, Heterophile / Q000276:immunology; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000276:immunology*; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D001667:Binding, Competitive; D004247:DNA / Q000276:immunology*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D000910:Antibodies, Heterophile; D000936:Antigen-Antibody Complex; D004247:DNA