title: Interaction of staphylococcal fibronectin binding protein (FnBP), TSST-1 and alpha-toxin with murine lymphocytes.
abstract: The most restrictive factor in the inductive phase of immune response is the contact and adhesion of antigen-presenting cells and responding lymphocytes. We report the in vitro formation of aggregates (clusters) of murine T lymphocytes and dendritic cells (DC) pulsed with highly purified staphylococcal toxins (TSST-1, alpha-toxin) and gene-cloned surface fibronectin binding protein (gal-FnBP). A high percentage of T lymphocytes were involved in clustering with TSST-1-pulsed DC. Lymphocytes of mice infected with Staphylococcus aureus (strain Cowan 1) 21 days before testing, clustered with the same ability as cells of mice infected 3 days before. Dendritic cells pulsed with alpha-toxin formed a moderate number of clusters only when co-cultured with T lymphocytes of mice infected 3 days before the test. FnBP-pulsed DC aggregated strongly with T lymphocytes. This response peaked at 3-4.5 h-cultures and increased again after 24 h. We concluded that at the beginning of co-culture, FnBP amplified the cells' adhesion process on antigen-independent way. This conclusion is supported by the observation that DC pulsed with formalin-modified FnBP (with a 65% decreased ability to bind fibronectin) formed fewer clusters with T-immune cells than dendritic cells pulsed with native, non-treated FnBP.
authors.affiliation: Department of Medical Microbiology, University of Lund, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Rozalska
authors.forename: T
authors.initials: T
authors.lastname: Wadström
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D002449:Cell Aggregation / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D004768:Enterotoxins / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000276:immunology / Q000378:metabolism; D018089:Superantigens; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D005353:Fibronectins; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; D010738:Type C Phospholipases