Platelet-derived growth factor induces c-fms and scavenger receptor genes in vascular smooth muscle cells.
Vascular smooth muscle cells proliferate and transform to foam cells in the process of atherosclerosis. In the present study, we demonstrated that platelet-derived growth factor (PDGF)-BB induced expression of proto-oncogene c-fms in vascular smooth muscle cells, which normally do not express c-fms, isolated from either human umbilical artery or rabbit aorta. No effect of the protein kinase C activator, phorbol ester, was demonstrated on mRNA expression of c-fms. In contrast, the scavenger receptor activity was induced by both PDGF-BB and phorbol ester. These results indicate that two characteristic genes of monocyte-macrophages were induced by PDGF-BB via the different pathways, and suggest that PDGF-BB plays an important role in initiating phenotypic conversion of smooth muscle cells to macrophage-like cells.
Inaba
T
T
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Gotoda
T
T
Shimano
H
H
Shimada
M
M
Harada
K
K
Kozaki
K
K
Watanabe
Y
Y
Hoh
E
E
Motoyoshi
K
K
Yazaki
Y
Y
D000818:Animals; D001161:Arteriosclerosis / Q000378:metabolism*; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D008297:Male; D008565:Membrane Proteins; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000502:physiology*; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics*; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B
C000715470:MAS1 protein, human; D008565:Membrane Proteins; D010982:Platelet-Derived Growth Factor; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D004247:DNA; D016186:Receptor, Macrophage Colony-Stimulating Factor