The complete sequence of a 9,543 bp segment on the left arm of chromosome III reveals five open reading frames including glucokinase and the protein disulfide isomerase.
We report here the DNA sequence of a 9.5 kb segment of chromosome III. The sequence was determined by subcloning the segment into subfragments generated by appropriate restriction enzymes followed by oligonucleotide-directed sequencing. The segment contains at least five open reading frames, YCL311, YCL312, YCL313, YCL314, YCL315. YCL311 and YCL315 extend in the adjacent fragments, A4H and A6C respectively. YCL312 encodes glucokinase, and YCL313 the protein disulfide isomerase. Disruption of YCL311, 314 and 315 by insertion of a URA3 cassette does not lead to a detectable phenotype, whereas disruption of YCL313 provokes cell lethality.
Scherens
B
B
Institut de Recherches du CERIA-COOVI, Université Libre de Bruxelles, Belgium.
Messenguy
F
F
Gigot
D
D
Dubois
E
E
D002872:Chromosome Deletion; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000737:chemistry* / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics* / Q000302:isolation & purification; D005800:Genes, Fungal / Q000235:genetics*; D005941:Glucokinase / Q000235:genetics* / Q000302:isolation & purification; D007535:Isomerases / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D019704:Protein Disulfide-Isomerases; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D004271:DNA, Fungal; D005656:Fungal Proteins; D005941:Glucokinase; D007535:Isomerases; D019704:Protein Disulfide-Isomerases