Nucleotide sequence of a cDNA for canine amylin.
Canine amylin cDNA was cloned and sequenced from a dog pancreas library using oligonucleotide probes corresponding to portions of human amylin. The sequence reported here is significantly different from an incomplete sequence previously reported. The non-amyloidogenic canine mature peptide is 89% and 95% identical to its amyloidogenic human and cat homologues, respectively. Both the dog and cat peptides are identical over the proposed amyloidogenic region, amino acids 20-29. Hydropathy plots comparing these three peptides are nearly identical suggesting that the primary sequence of amylin is in itself insufficient to explain pancreatic amyloid formation.
Albrandt
K
K
Department of Molecular Biology, Amylin Corporation, San Diego, CA 92121.
Mull
E
E
Cooper
G J
GJ
Johnson
M J
MJ
D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004285:Dogs; D058228:Islet Amyloid Polypeptide; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D000682:Amyloid; D015342:DNA Probes; D058228:Islet Amyloid Polypeptide; D009838:Oligodeoxyribonucleotides; D011498:Protein Precursors; D004247:DNA