Specific hybridization probes for mouse alpha 2(IX) and alpha 1(X) collagen mRNAs.
We have used polymerase chain reaction (PCR) technology and available cross-species sequence information to construct cDNA probes for mouse alpha 2(IX) and alpha 1(X) collagen transcripts. Sequencing confirmed the identification of the clones. Northern analysis proved sufficient divergence of the cloned sequences from other collagen transcripts: specific detection of the mouse 2.9 kb alpha 2(IX) and 3.3 kb alpha 1(X) collagen mRNAs was seen under normal hybridization and washing conditions.
Elima
K
K
University of Turku, Department of Medical Biochemistry, Finland.
Metsäranta
M
M
Kallio
J
J
Perälä
M
M
Eerola
I
I
Garofalo
S
S
De Crombrugghe
B
B
Vuorio
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003094:Collagen / Q000737:chemistry / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*
D015342:DNA Probes; D012333:RNA, Messenger; D003094:Collagen