Bodo caudatus medRNA and 5S rRNA genes: tandem arrangement and phylogenetic analyses.
The Bodo caudatus mini-exon-derived RNA gene repeat has been isolated following PCR amplification. The DNA sequence of the mini-exon fits the trypanosomatid mini-exon consensus, supporting inclusion of Bodo in this group. The B. caudatus mini-exon repeat also contains the 5S ribosomal RNA gene, an organization found in the trypanosome T. rangeli and five genera of nematodes. Phylogenetic analysis of both mini-exon-derived RNA gene and 5S gene sequences show that the free-living B. caudatus is more closely related to the monogenetic Crithidia than the digenetic Trypanosoma. Similarity between the Euglena gracilis trans-spliced leader and trypanosomatid mini-exon sequences was also noted during these comparisons.
Campbell
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Department of Microbiology and Immunology, University of California, Los Angeles 90024.
D000818:Animals; D001483:Base Sequence; D003421:Crithidia / Q000235:genetics; D004247:DNA / Q000235:genetics; D005056:Euglena gracilis / Q000235:genetics; D056890:Eukaryota / Q000235:genetics*; D005091:Exons; D015321:Gene Rearrangement; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014345:Trypanosoma / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D012341:RNA, Ribosomal, 5S; D004247:DNA