title: The gene for the S7 ribosomal protein of Chlamydia trachomatis: characterization within the chlamydial str operon.
abstract: The prokaryotic ribosomal operon, str, contains open reading frames for the two elongation factors, elongation factor G (EF-G) and elongation factor Tu (EF-Tu), and ribosomal proteins S7 and S12. The DNA sequence and predicted amino acid sequence for S7 from Chlamydia trachomatis are presented and compared with homologues from other prokaryotes. Also, the relationship of the S7 gene to the open reading frames for ribosomal protein S12 and EF-G is described. Significant amino acid homology is also noted when the amino-terminal sequence of chlamydial EF-G is compared with the cytoplasmic tetracycline resistance factors, tetM and tetO, from streptococci and Campylobacter jejuni. Related findings and possible resistance mechanisms for the newly recognized tetracycline-resistant clinical isolates of C. trachomatis are discussed.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of California, Los Angeles 90024-1732.
authors.forename: E A
authors.initials: EA
authors.lastname: Wagar
authors.forename: M
authors.initials: M
authors.lastname: Pang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002692:Chlamydia trachomatis / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors / Q000235:genetics; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D004269:DNA, Bacterial; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D012269:Ribosomal Proteins; C037442:ribosomal protein S7