[Effects of McAbs HIP2, APT4 and HI117 on the human platelet cytoskeleton].
The effects of monoclonal antibodies HIP2, APT4 and HI117 on the platelet cytoskeleton were investigated. The results showed that HIP2, APT4 and HI117 could induce an increase of platelet cytoskeleton materials in PRP. The action of HIP2 and APT4 could be obviously inhibited by the calmodulin inhibitor EBB. The inhibition rates of EBB were 76.3% and 48.95%, respectively. But EBB couldn't inhibit the HI117-induced an increase of platelet cytoskeleton materials. EBB could completely inhibit APT4-induced platelet aggregation(100%) and partially inhibit HIP2-induced platelet aggregation (14.26%). It couldn't inhibit HI117-induced platelet aggregation. These McAbs couldn't induce aggregation and actin mobilization in washed platelets in the presence of EDTA. The results indicate that platelet aggregation and the increase of actin induced by these McAbs, in addition to Ca2+, could be related to a factor or factors in the plasma. The Ca(2+)-CM system might play an important role in platelet aggregation and actin mobilization induced by HIP2 and APT4.
Sun
H
H
Institute of Hematology, Tianjin.
Li
J
J
D000199:Actins / Q000097:blood; D000328:Adult; D000911:Antibodies, Monoclonal / Q000502:physiology*; D001792:Blood Platelets / Q000276:immunology*; D003599:Cytoskeleton / Q000378:metabolism*; D006801:Humans; D010974:Platelet Aggregation
D000199:Actins; D000911:Antibodies, Monoclonal