title: Nucleotide sequence of a cDNA for canine amylin.
abstract: Canine amylin cDNA was cloned and sequenced from a dog pancreas library using oligonucleotide probes corresponding to portions of human amylin. The sequence reported here is significantly different from an incomplete sequence previously reported. The non-amyloidogenic canine mature peptide is 89% and 95% identical to its amyloidogenic human and cat homologues, respectively. Both the dog and cat peptides are identical over the proposed amyloidogenic region, amino acids 20-29. Hydropathy plots comparing these three peptides are nearly identical suggesting that the primary sequence of amylin is in itself insufficient to explain pancreatic amyloid formation.
authors.affiliation: Department of Molecular Biology, Amylin Corporation, San Diego, CA 92121.
authors.forename: K
authors.initials: K
authors.lastname: Albrandt
authors.forename: E
authors.initials: E
authors.lastname: Mull
authors.forename: G J
authors.initials: GJ
authors.lastname: Cooper
authors.forename: M J
authors.initials: MJ
authors.lastname: Johnson
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004285:Dogs; D058228:Islet Amyloid Polypeptide; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000682:Amyloid; D015342:DNA Probes; D058228:Islet Amyloid Polypeptide; D009838:Oligodeoxyribonucleotides; D011498:Protein Precursors; D004247:DNA