Regulation of ribosomal protein S25 in HL60 cells isolated for resistance to adriamycin.
The ribosomal protein S25 gene is highly overexpressed in HL60 cells isolated for resistance to adriamycin. In contrast there is no overexpression of 3 other ribosomal genes which code for proteins S14, S17 and S24. Studies with an antibody against a synthetic peptide of the S25 protein show that although the S25 gene is overexpressed in resistant cells there is no corresponding increase in the levels of S25 protein. These results suggest that the r-protein levels are highly regulated by translational controls or protein turnover.
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Division of Biology, Kansas State University, Manhattan 66506.
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D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D015342:DNA Probes; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D015342:DNA Probes; C489168:RPS25 protein, human; D012269:Ribosomal Proteins; D004317:Doxorubicin