Tumoricidal properties of rat peritoneal macrophages activated with various activators depend on nitrogen oxid synthesis.
Cytolytic activity of mineral oil elicited rat peritoneal macrophages activated by lipopolysaccharide (LPS) and/or rIFN-gamma, rIL-2, Zymosan and PMA (4-beta-phorbol-12-beta-myristate 13-alpha-acetate) was detected in the presence of various concentrations of L-arginine. This paralleled the NO2- production in the presence, but not in the absence, of L-arginine. Significant amount of NO2- was detected in the peritoneal macrophages cultured with 0.4 mmol of L-arginine 8 days and the last 24 h with LPS at a concentration of 1 microgram/ml. No significant differences were found between activated peritoneal macrophages obtained from normal (healthy) and/or from tumor bearing rats to induce tumoricidal activity and NO2- production under the same experimental conditions. The results showed that the major cytolytic mechanism against BP6-Tu2 and U 937 tumor cell lines is L-arginine-dependent nitrogen oxide synthesis of activated rat peritoneal macrophages.
Kusenda
J
J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Kalafut
F
F
Klobusická
M
M
Novotná
L
L
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003470:Culture Media; D005260:Female; D007167:Immunotherapy; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D008070:Lipopolysaccharides; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000628:therapy*; D009573:Nitrites / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D002860:Chromium Radioisotopes; D003470:Culture Media; D007376:Interleukin-2; D008070:Lipopolysaccharides; D009573:Nitrites; D011994:Recombinant Proteins; D007371:Interferon-gamma; D015054:Zymosan; D001120:Arginine; D013755:Tetradecanoylphorbol Acetate