Anti-vimentin monoclonal antibody recognizes a cell with dendritic appearance in the chicken's bursa of Fabricius.
The bursa of Fabricius was studied by immunohistochemical method using anti-vimentin monoclonal antibody (clone 3B4). This monoclonal antibody identified a vimentin positive cell in the medulla of the bursal follicle. During the first 2 weeks of life the vimentin positive cells located along the corticomedullary border and later became prominent in the medulla with the exception of a narrow zone adjacent to the corticomedullary border. After hatching the accumulation of vimentin-type intermediate filaments on one side of the nucleus endowed the vimentin positive cells with a polarized appearance. This "cap-like" vimentin positive area of the cytoplasm determined the position of the major cell process. Within the medulla the Ia positive secretory dendritic cells contained secretory granules in one of the cell processes. The distribution, shape, and polarized appearance of the vimentin positive cells were identical with that of the secretory dendritic cells. Therefore, the anti-vimentin monoclonal antibody proved to be useful for identification of the bursal secretory dendritic cells. During rapid bursal growth the number of secretory dendritic cells increased, possibly, by proliferation of vimentin negative secretory dendritic cell precursors located along the corticomedullary border.
Olah
I
I
Poultry Science Department, Clemson University, South Carolina 29634-0379.
Kendall
C
C
Glick
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D002060:Bursa of Fabricius / Q000166:cytology / Q000378:metabolism*; D002645:Chickens; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D014746:Vimentin / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D014746:Vimentin