Atrial natriuretic peptide (ANP): a study of ANP and its mRNA in cardiocytes, and of plasma ANP levels in non-obese diabetic mice.
Atrial natriuretic peptide (ANP) levels in cardiocytes and plasma were examined by using immunohistochemistry, electron microscopy, and radioimmunoassay in non-obese diabetic mice (NOD). Cardiocyte ANP mRNA expression was measured by the polymerase chain reaction method. ANP immunoreactivity in the auricular cardiocytes was more prominent in hyperglycemic mice (NOD-h) than in normoglycemic mice (NOD-n). Ultrastructural examination showed that auricular cardiocytes of the NOD-h group contained more cytoplasmic granules than cells of the NOD-n group. Ultrastructural morphometry indicated that the number of granules per auricular cardiocyte was significantly larger in the NOD-h group than in the NOD-n group (P less than 0.01), whereas the granule diameter was significantly smaller in the NOD-h group (P less than 0.01). Radioimmunoassay showed that ANP levels in the NOD-h auricular cardiocytes were significantly higher than those in the NOD-n cardiocytes (P less than 0.01); the opposite was true in plasma. Cardiocyte ANP mRNA expression was lower in the NOD-h group than in the NOD-n group.
Mifune
H
H
Institute of Animal Experiment, Kurume University School of Medicine, Fukuoka, Japan.
Suzuki
S
S
Honda
J
J
Kobayashi
Y
Y
Noda
Y
Y
Hayashi
Y
Y
Mochizuki
K
K
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis* / Q000097:blood; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005246:Feedback; D005260:Female; D005786:Gene Expression Regulation; D006325:Heart Atria; D006943:Hyperglycemia / Q000378:metabolism / Q000473:pathology; D051379:Mice; D016688:Mice, Inbred NOD / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor