Biochemical parameters which may be useful in improving organ viability after storage.
Biochemical methods that accurately detect ischemic damage in livers stored by flush cooling would be useful in the efficient development of new storage solutions. This study compares UW and Collins' solutions to evaluate those biochemical parameters which may be useful in assessing the reversibility of ischemic damage and the efficiency of organ storage solutions. Livers stored in UW solution showed higher levels of adenine nucleotides at all storage times studied. This increase in adenine nucleotides averaged 29% and was statistically significant. There was also a significant increase in the NAD levels in organs stored in UW solutions; however, the ATP levels were not significantly different after storage in either solution. Storage of livers in UW solution also decreased the amount of DNA damage which occurs with storage as compared to storage in Collins' solution, becoming statistically significant after 48 hr of storage. This suggests that these biochemical parameters may be useful in designing improved storage solutions since they reflect the extent of ischemic damage to the organ and can be determined on a small section of liver taken by biopsy.
Warnick
C T
CT
Department of Surgery, LDS Hospital, Salt Lake City, Utah 84143.
Stevens
M H
MH
Richards
K F
KF
Rees
W V
WV
Belnap
L P
LP
Stevens
L E
LE
D000227:Adenine Nucleotides / Q000032:analysis; D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D005978:Glutathione; D006982:Hypertonic Solutions / Q000494:pharmacology*; D007328:Insulin; D008099:Liver / Q000737:chemistry / Q000166:cytology*; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011887:Raffinose; D012996:Solutions / Q000494:pharmacology*; D013552:Swine; D013556:Swine, Miniature
D000227:Adenine Nucleotides; C024102:Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D004247:DNA; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose