[Study of the structure of the duplex (Phn-NH(CH2)2NH)pd(CCAAACA) .pd(TGTTTGGC) with covalently bound 10-(2-hydroxyethyl)phenazine in an aqueous solution by 2D-1H-NMR spectroscopy].
The spatial structure of duplex (Phn-NH(CH2)2NH)pd(CCAAACA).pd(TGTTTGGC) having a N-(2-oxyethyl)-phenazinium residue covalently linked with the 5'-terminal phosphate of the heptanucleotide was studied by means of one- and two-dimensional 1H-NMR spectroscopy. The resonances of phenazinium protons, ethylenediamine linker protons, as well as, oligonucleotide H5/H6/H8/CH3 base protons and H1',H2'a, H2'b, H3', H4' deoxyribose protons have been assigned by means of 1H-COSY, 1H-NOESY and 1H-13C-COSY. The presence of the phenazine residue in duplex causes an additional imino proton signal of the terminal (G-7).(C-1) base pair, suggesting a higher stability of the duplex (Phn-NH(CH2)2NH)pd(CCAAACA).pd(TGTTTGGC) as compared to the unmodified duplex pd(CCAAACA).pd(TGTTTGGC). Analysis of NOE interactions between protons of the dye and the oligonucleotides show the phenazinium polycyclic system to intercalate between G-7 and C-8 residues of the octanucleotide.
Bichenkova
E V
EV
Zarytova
V F
VF
Ivanova
E M
EM
Mal'tseva
T V
TV
Sal'nikov
G E
GE
Lebedev
A V
AV
D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D010619:Phenazines / Q000737:chemistry*; D012996:Solutions; D014867:Water
D007202:Indicators and Reagents; D010619:Phenazines; D012996:Solutions; D014867:Water; C076555:10-(2-hydroxyethyl)phenazine
Issledovanie struktury dupleksa (Phn-NH(CH2)2NH)pd(CCAAACA.pd(TGTTTGGC)s kovlentno prisoedinennym 10-(2-gidroksiétil)fenaziniem v vodnom rostvore metodom 2M-1H-IaMR-spektroskopii.