Both the anaerobic pathway and aerobic desaturation are involved in the synthesis of unsaturated fatty acids in Vibrio sp. strain ABE-1.
Vibrio sp. strain ABE-1 is a unique marine bacterium in terms of its ability to synthesize delta 9-trans-hexadecenoic acid and delta 7-cis-tetradecenoic acid (14:1(7c); Okuyama, H., Sasaki, S., Higashi, S. and Murata, N. (1990) J. Bacteriol. 172, 3515-3518). The present study, involving labeling with [1-14C]acetate, demonstrated that 14:1 is synthesized by the anaerobic pathway. When cells of this bacterium were grown in the presence of [1-14C]myristic acid (14:0), this compound was converted to palmitic (16:0) and hexadecenoic (16:1) acids but not to 14:1, under aerobic conditions. These results suggest that the incorporated 14:0 was elongated to 16:0 and then converted to 16:1 by the aerobic desaturation of 16:0. It appears that the anaerobic pathway and aerobic desaturation are both involved in the synthesis of unsaturated fatty acids during aerobic growth of Vibrio sp. strain ABE-1.
Morita
N
N
Department of Biology, Faculty of Science, Hokkaido University, Sapporo, Japan.
Gotoh
M
M
Okajima
N
N
Okuyama
H
H
Hayashi
H
H
Higashi
S
S
Murata
N
N
D000085:Acetates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004952:Esters; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D019814:Myristic Acid; D009227:Myristic Acids / Q000737:chemistry; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000737:chemistry; D014733:Vibrio / Q000254:growth & development / Q000378:metabolism*
D000085:Acetates; D004952:Esters; D005231:Fatty Acids, Unsaturated; D009227:Myristic Acids; D010169:Palmitic Acids; D019814:Myristic Acid; D019308:Palmitic Acid