DNA binding sites 5' of the IgG1 switch region comprising IL4 inducibility and B cell specificity.
Immunoglobulin class switch recombination is directed to the same switch region on both chromosomes of a B cell by an as yet unknown mechanism. The cytokine interleukin 4 (IL4) targets recombination in activated B lymphocytes to the gamma 1 switch region (s gamma 1). Here we report two DNA-binding-proteins which bind to a sequence 5' of s gamma 1. One protein is B cell specific, while binding of the other one is induced by IL4. These two proteins bind to a region 700 bp upstream of the putative promoter region of the gamma 1 germline transcripts and may be involved in the process of recombination and/or transcription.
Illges
H
H
Institut für Genetik, Universität zu Köln, Germany.
Radbruch
A
A
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000502:physiology*; D007074:Immunoglobulin G / Q000235:genetics*; D007134:Immunoglobulin Switch Region / Q000235:genetics*; D015847:Interleukin-4 / Q000502:physiology*; D008212:Lymphocyte Depletion; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D015847:Interleukin-4