Peptide analogues of consensus receptor sequence inhibit the action of cytokines on human basophils.
A novel cytokine receptor family has been defined based on structural homologies between the extracellular receptor domains. All receptors share a WSXWS consensus sequence just outside the plasma membrane. To evaluate whether this box of homology has a role in receptor function we synthesized peptides encompassed within the WSXWS region. In a basophil mediator release assay, three peptides inhibited the priming effect of interleukin (IL)-3, IL-5, and granulocyte/macrophage colony-stimulating factor (GM-CSF) on synthesis of leukotriene C4 induced by chemotactic peptides. The study suggests that, analogous to the RGD motif in adhesive proteins, the WSXWS motif serves as a recognition site for functional protein-protein interaction of cytokine receptors.
Bischoff
S C
SC
Institute of Clinical Immunology, Inselspital, Bern, Switzerland.
de Weck
A L
AL
Dahinden
C A
CA
D000595:Amino Acid Sequence; D001491:Basophils / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D015848:Interleukin-5 / Q000494:pharmacology; D008969:Molecular Sequence Data; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D016184:Receptors, Colony-Stimulating Factor / Q000737:chemistry* / Q000502:physiology; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry / Q000502:physiology; D011971:Receptors, Immunologic / Q000737:chemistry / Q000502:physiology; D018123:Receptors, Interleukin; D016185:Receptors, Interleukin-3 / Q000737:chemistry / Q000502:physiology; D053649:Receptors, Interleukin-5; D013189:SRS-A / Q000096:biosynthesis*
D007377:Interleukin-3; D015848:Interleukin-5; D009842:Oligopeptides; D016184:Receptors, Colony-Stimulating Factor; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D011971:Receptors, Immunologic; D018123:Receptors, Interleukin; D016185:Receptors, Interleukin-3; D053649:Receptors, Interleukin-5; D013189:SRS-A; D009240:N-Formylmethionine Leucyl-Phenylalanine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor