Effects of isoflurane on transport across the blood-brain barrier.
The blood-brain barrier (BBB) plays an important role in regulating and restricting transfer of molecules into the interstitial space of the brain. Anesthetic agents may affect the permeability of the BBB. In this investigation, the effect of isoflurane on the transport of small hydrophilic molecules across the BBB was studied in rats by measuring the blood-brain transfer coefficient (Ki) and the regional cerebral blood flow (rCBF) and by calculating the capillary permeability-surface area (PS) product. In the control group, after a femoral artery and vein were catheterized under isoflurane anesthesia, rats were allowed to remain awake for 2 h before measuring Ki (n = 6) or rCBF (n = 11). In the two experimental groups, rats were anesthetized with 1% and 2% isoflurane respectively, and their lungs were mechanically ventilated through a tracheal tube. Ki was measured (1% n = 6, 2% n = 7) using 14C-alpha-aminoisobutyric acid, and rCBF was measured (2% n = 7) using 14C-iodoantipyrine. Two percent isoflurane did not affect rCBF in 9 of 13 brain regions. Blood flow was less in the lateral and posterior cortex and greater in the medulla and pons when compared with the control group. Ki was less in 11 of 13 brain regions in both the 1% and 2% isoflurane groups than in the control group. There was no significant difference between 1 and 2% isoflurane in any brain region.(ABSTRACT TRUNCATED AT 250 WORDS)
Chi
O Z
OZ
Department of Anesthesia, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick 08903-0019.
Anwar
M
M
Sinha
A K
AK
Wei
H M
HM
Klein
S L
SL
Weiss
H R
HR
D000621:Aminoisobutyric Acids / Q000493:pharmacokinetics; D000769:Anesthesia, Inhalation; D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000502:physiology; D002199:Capillary Permeability / Q000187:drug effects; D002250:Carbon Radioisotopes; D002560:Cerebrovascular Circulation / Q000187:drug effects; D007530:Isoflurane / Q000494:pharmacology*; D008297:Male; D051381:Rats
D000621:Aminoisobutyric Acids; D002250:Carbon Radioisotopes; D007530:Isoflurane; D000983:Antipyrine; C008135:iodoantipyrine