A supercoil-dependent structural alteration within the regulatory region of the human transferrin receptor gene.
The transferrin receptor gene is transcribed at low levels in quiescent cells and at much higher levels in growing or transformed cells. This regulation involves elements located within the first 114 base pairs upstream of the major transcriptional start site. This region is specifically recognized by several transacting factors and contains an element that is composed of alternating purines and pyrimidines. In vitro this element can adopt a non-B DNA conformation in a supercoil-dependent manner. Similar elements, with nearly identical spacing relative to a protein recognition sequence, can be observed in several other proliferation dependent gene promoters.
Ouyang
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University of South Carolina, Department of Biological Sciences, Columbia 29208.
Miskimins
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D001483:Base Sequence; D004278:DNA, Superhelical / Q000187:drug effects; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D011990:Receptors, Transferrin / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*
D004278:DNA, Superhelical; D011990:Receptors, Transferrin; D004047:Diethyl Pyrocarbonate