title: Appearance of thymosin alpha 1 in supernatants of monocytes incubated with prothymosin alpha.
abstract: Prothymosin alpha, a polypeptide of 109 to 111 amino acid residues, contains the entire thymosin alpha 1 sequence (residues 1-28) at its amino terminal. Human peripheral blood monocytes incubated with prothymosin alpha release thymosin alpha 1 in the culture supernatants. In addition total RNA is found to increase. The production of thymosin alpha 1 involves de novo protein synthesis as shown by the kinetics of this release and its inhibition by actinomycin D and cycloheximide. Thymosin alpha 1 release, possibly in association with HLA-DR, stimulates the proliferation of the T cell population.
authors.affiliation: Laboratory of Biological Chemistry, University of Ioannina Medical School, Greece.
authors.forename: S
authors.initials: S
authors.lastname: Frillingos
authors.forename: K
authors.initials: K
authors.lastname: Seferiadis
authors.forename: M
authors.initials: M
authors.lastname: Papanastasiou
authors.forename: C N
authors.initials: CN
authors.lastname: Baxevanis
authors.forename: M
authors.initials: M
authors.lastname: Frangou-Lazaridis
authors.forename: M
authors.initials: M
authors.lastname: Economou
authors.forename: M
authors.initials: M
authors.lastname: Papamichail
authors.forename: O
authors.initials: O
authors.lastname: Tsolas
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007700:Kinetics; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine; D013601:T-Lymphocytes / Q000378:metabolism; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D013997:Time Factors
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; C040782:prothymosin alpha; D013947:Thymosin; D000077596:Thymalfasin