Cross-reactivity of highly purified okadaic acid (OA), synthetic, spiroketal East sphere of OA and ciguatoxin.
This study presents data from the cross-reactivity analysis of purified ciguatoxin (CTX), okadaic acid (OA), and the East sphere or Fragment B-C of OA with their homologous antibodies, monoclonal antibodies to ciguatoxin (MAb-CTX) and okadaic acid (MAb-OA). The test system used was the stick enzyme immunoassay. MAb-CTX gave peak titers of 1.5ng, 10ng and 50ng respectively for CTX, East sphere and OA. Competitive inhibition analysis showed that 4ng purified CTX blocked completely MAb-CTX reaction with crude CTX, OA and East sphere of OA blocked at similar concentrations (approximately 50ng). The activity with MAb-OA in the homologous system with OA and East sphere was insignificant. This may be attributable to the improper concentrations used. The cross-reactivity between MAb-CTX with OA and its Fragment B-C may cause difficulty in the test system in its application to assess toxic fish due to ciguatoxin.
Hokama
Y
Y
Department of Pathology, JABSOM, University of Hawaii, Honolulu 96822.
Hong
T W
TW
Isobe
M
M
Ichikawa
Y
Y
Yasumoto
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002922:Ciguatoxins / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D004988:Ethers, Cyclic / Q000737:chemistry / Q000276:immunology*; D007120:Immunochemistry; D007124:Immunoenzyme Techniques; D008387:Marine Toxins / Q000737:chemistry / Q000276:immunology; D015394:Molecular Structure; D019319:Okadaic Acid
D000911:Antibodies, Monoclonal; D004988:Ethers, Cyclic; D008387:Marine Toxins; D002922:Ciguatoxins; D019319:Okadaic Acid