LBL, a novel, developmentally regulated, laminin-binding lectin.
A 190/220-kDa complex found in integrin preparations was purified, and monoclonal antibodies were raised against it. The immunoaffinity-purified complex appears to be a trimer of very similar or identical 70-kDa subunits. It is a novel extracellular matrix molecule as determined by its subunit composition, N-terminal amino acid sequence, and in vivo localization. It is distributed widely in basement membranes including those from muscle, nerve, and kidney. It is also present in connective tissue regions such as perineurium and perimysium. It has the unusual property that it is initially expressed very late in avian development near the time of hatching. This protein is found to copurified with integrin because it binds to the carbohydrate support in Sepharose. Hemagglutination assays with mono- and disaccharides show that it functions as a lectin with galactoside-binding specificity. This protein is also found to bind strongly and specifically to laminin at a site distinct from its lectin activity, but does not bind to fibronectin or type IV collagen. The protein appears to be conserved and is a common contaminant of many laminin preparations. We call this novel protein "LBL" for laminin-binding lectin.
Bao
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Department of Cell and Structural Biology, University of Illinois, Urbana 61801.
Muschler
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J
Horwitz
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AF
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002645:Chickens; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005697:Galactosides / Q000378:metabolism; D006386:Hemagglutination Tests; D016023:Integrins / Q000378:metabolism; D007797:Laminin / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000276:immunology / Q000378:metabolism*; D017474:Receptors, Laminin; D013379:Substrate Specificity; D014018:Tissue Distribution
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D016326:Extracellular Matrix Proteins; D005697:Galactosides; D016023:Integrins; D007797:Laminin; D037102:Lectins; D011971:Receptors, Immunologic; D017474:Receptors, Laminin