title: The structure of two IgMs showing different activity from a patient with Waldenstrom's macroglobulinaemia.
abstract: The two monoclonal IgMs (IgM1 and IgM2) were characterized from a patient Waldenström's macroglobulinaemia that resulted in a gammapathy. Heavy and light chains were isolated from the IgM. The complete primary structure of the two light chains and the NH2-terminal region of the two heavy chain molecules were determined. The sequence data indicated that the heavy and light chains from both IgMs belong to the same (III and II) lambda subgroups. By testing their antibody activity it was found by immunofluorescence and immunoblotting that only IgM2 reacts with an intermediate filament protein.
authors.affiliation: Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Méndez
authors.forename: C
authors.initials: C
authors.lastname: Osuna
authors.forename: A
authors.initials: A
authors.lastname: Sánchez
authors.forename: Y
authors.initials: Y
authors.lastname: Revilla
authors.forename: F
authors.initials: F
authors.lastname: Soriano
authors.forename: C
authors.initials: C
authors.lastname: Montalbán
authors.forename: J
authors.initials: J
authors.lastname: Seguí
authors.forename: J
authors.initials: J
authors.lastname: Avila
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005803:Genes, Immunoglobulin / Q000276:immunology; D006801:Humans; D006942:Hypergammaglobulinemia / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry; D007147:Immunoglobulin Light Chains / Q000737:chemistry; D007075:Immunoglobulin M / Q000737:chemistry* / Q000276:immunology; D007382:Intermediate Filaments / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000276:immunology; D013329:Structure-Activity Relationship; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology*
chemical_list: D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M