Characterization of the cDNA and genomic sequence of a G protein gamma subunit (gamma 5).
A cDNA from human placenta and liver tissues that contained both sequence for the lysosomal glycosidase di-N-acetylchitobiase and sequence homologous to the gamma subunit of GTP-binding proteins was previously isolated. Here we have shown that the gamma-subunit-homologous portion of this unusual cDNA is derived from a member of the gamma-subunit multigene family. The partial human gamma-subunit sequence was used to isolate the corresponding full-length cDNA clones from bovine and rat livers. The two cDNAs encode identical 68-amino-acid proteins (7.3 kDa) homologous to previously cloned G protein gamma subunits. The bovine gene sequence encoding this new gamma-subunit isoform (gamma 5) was determined and found to have an intron-exon structure consistent with the original human chitobiase-gamma 5-subunit hybrid mRNA being a product of alternative splicing. Genomic cloning also resulted in the isolation of a human gamma 5 pseudogene.
Fisher
K J
KJ
Department of Molecular and Cell Biology, Althouse Laboratory, Pennsylvania State University, University Park 16802.
Aronson
N N
NN
D000118:Acetylglucosaminidase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D005091:Exons; D019204:GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D007438:Introns; D008099:Liver / Q000737:chemistry; D008957:Models, Genetic; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D011544:Pseudogenes / Q000235:genetics; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D012322:RNA Precursors; D000118:Acetylglucosaminidase; D019204:GTP-Binding Proteins