In vitro protein synthesis and alpha amylase activity in F cells from hepatopancreas of Palaemon serratus (Crustacea; Decapoda).
In crustaceans, all the steps in the assimilation of food take place in the hepatopancreas. To facilitate the study of this organ, a method for the dissociation of cell types was developed. The hepatopancreas of the prawn Palaemon serratus was mechanically dissociated and the cells separated by Percoll density-gradient centrifugation. The E and R cells had similar densities of around 1.05 g/ml. The F cells were separated into two distinct fractions with densities of 1.075 and 1.082 g/ml. The B cells sedimented at a density of 1.12 g/ml. The ratio between the two populations of F cells was found to vary during the intermolt cycle while B cells disappeared after the molt. When the density gradient fractions were incubated with 3H-leucine, incorporation was highest in the F cell fractions. Measurements of alpha-amylase activity, indicated that the two populations of F cells may be derived from the same cell type.
Toullec
J Y
JY
Laboratoire de Biologie Marine du Collège de France, Concarneau.
Chikhi
M
M
van Wormhoudt
A
A
D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D004064:Digestive System / Q000378:metabolism*; D007930:Leucine; D008099:Liver / Q000166:cytology / Q000378:metabolism; D033541:Palaemonidae; D010179:Pancreas / Q000166:cytology / Q000378:metabolism; D014176:Protein Biosynthesis; D000516:alpha-Amylases / Q000378:metabolism*
D000516:alpha-Amylases; D007930:Leucine