Neuronal cells mature faster on polyethyleneimine coated plates than on polylysine coated plates.
Cell morphology, protein/DNA ratio, ganglioside analysis, taurine uptake, and the activity of neurone specific enolase showed that neuronal cells mature faster when grown on polyethyleneimine coated plates compared to cells grown on polylysine coated plates. Our results also show higher protein/DNA ratio and total and neuron specific enolase activities in cells grown in serum supplemented medium, when compared to their counterparts grown in synthetic medium. Moreover, our results show that only some specific markers can be used to determine the early and late events of cell maturation, whereas other markers continuously vary with time.
Lelong
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Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.
Petegnief
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Rebel
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D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D002855:Chromatography, Thin Layer; D003470:Culture Media; D003584:Cytological Techniques; D004247:DNA / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011094:Polyethyleneimine; D011107:Polylysine; D011506:Proteins / Q000378:metabolism; D013654:Taurine / Q000378:metabolism
D002395:Catecholamines; D003470:Culture Media; D005732:Gangliosides; D011506:Proteins; D013654:Taurine; D011107:Polylysine; D011094:Polyethyleneimine; D004247:DNA; D010751:Phosphopyruvate Hydratase