Inhibition of benzo[a]pyrene-induced mouse forestomach neoplasia by a principal flavor component of Japanese-style fermented soy sauce.
A refined diet supplemented with Japanese-style fermented soy sauce (shoyu) inhibits benzo[a]pyrene-induced forestomach neoplasia in mice (Cancer Res., 51:2940-2942, 1991). In the present study, soy sauce was extracted with ethyl acetate. The soluble fraction contained flavor/aroma compounds and antioxidants, whereas amino-carbonyl compounds that impart color were concentrated in the ethyl acetate insoluble fraction. Both fractions inhibited benzo[a]pyrene-induced forestomach neoplasia in a protocol in which the test material was fed following exposure to the carcinogen. A principal flavor/aroma component of soy sauce, 4-hydroxy-2(or 5)-ethyl-5(or 2)-methyl-3(2H)-furanone, was fed to mice following benzo[a]pyrene administration and found to inhibit the subsequent development of forestomach neoplasia. 4-Hydroxy-2(or 5)-ethyl-5(or 2)-methyl-3(2H)-furanone was effective when fed at 4 mg/kg body weight/day, indicating that it is a potent anticarcinogen.
Nagahara
A
A
Department of Food Microbiology and Toxicology, University of Wisconsin-Madison 53706.
Benjamin
H
H
Storkson
J
J
Krewson
J
J
Sheng
K
K
Liu
W
W
Pariza
M W
MW
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000302:isolation & purification; D001564:Benzo(a)pyrene / Q000633:toxicity*; D004032:Diet; D005260:Female; D005285:Fermentation; D005663:Furans / Q000302:isolation & purification / Q000627:therapeutic use*; D007564:Japan; D051379:Mice; D008813:Mice, Inbred ICR; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000517:prevention & control*
D000970:Antineoplastic Agents; D000975:Antioxidants; D005663:Furans; D001564:Benzo(a)pyrene