A Streptomyces glaucescens endodeoxyribonuclease which shows a strong preference for CC dinucleotide.
We have characterized a deoxyribonuclease from Streptomyces glaucescens that cleaves double-stranded DNA preferably between the dinucleotide 5'-CC-3'. The cleavage specificity was demonstrated by both analysis of the terminal nucleotides of the generated fragments and DNA sequencing of partially digested DNA. Digestion of lambda DNA with this enzyme resulted in the production of double-stranded fragments with 5' and/or 3'-protruding single-stranded tails. DNase I footprinting experiments indicated that the nuclease specifically binds to its cleavage sites on the DNA under non-catalytic conditions. The enzyme is not affected by cytosine methylation in hemimethylated DNA.
Aparicio
J F
JF
Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
Freije
J M
JM
Lopez-Otin
C
C
Cal
S
S
Sanchez
J
J
D010582:Bacteriophage lambda; D001483:Base Sequence; D001665:Binding Sites; D003596:Cytosine; D004279:DNA, Viral / Q000378:metabolism; D015226:Dinucleoside Phosphates; D004706:Endodeoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010761:Phosphorus Radioisotopes; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
D004279:DNA, Viral; D015226:Dinucleoside Phosphates; D010761:Phosphorus Radioisotopes; D003596:Cytosine; D004706:Endodeoxyribonucleases