title: Nucleotide sequence of the cDNA encoding the common alpha subunit of the chicken pituitary glycoprotein hormones.
abstract: Recombinant cDNA clones that encode the alpha subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754 bp in length. It contained 81 nucleotides of the 5'-untranslated region (UTR), an open-reading frame of 360 bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature alpha subunit, and 268 nucleotides of the 3'-UTR, followed by a 45 bp poly(A) tract. There was 69-79% homology between the nucleotide sequence of the coding region for the chicken and mammalian alpha-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp alpha-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I.
authors.affiliation: Department of Poultry Science, Ohio State University, Wooster 44691.
authors.forename: D N
authors.initials: DN
authors.lastname: Foster
authors.forename: D
authors.initials: D
authors.lastname: Galehouse
authors.forename: T
authors.initials: T
authors.lastname: Giordano
authors.forename: B
authors.initials: B
authors.lastname: Min
authors.forename: I C
authors.initials: IC
authors.lastname: Lamb
authors.forename: D A
authors.initials: DA
authors.lastname: Porter
authors.forename: K J
authors.initials: KJ
authors.lastname: Intehar
authors.forename: W L
authors.initials: WL
authors.lastname: Bacon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004247:DNA / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D010907:Pituitary Hormones / Q000235:genetics*; D011487:Protein Conformation
chemical_list: D006023:Glycoproteins; D010907:Pituitary Hormones; D004247:DNA