A kinetic study of the generation and decomposition of some phenothiazine free radicals formed during enzymatic oxidation of phenothiazines by peroxidase-hydrogen peroxide.
A kinetic study of the oxidation of four different phenothiazines (Pts) by peroxidase-hydrogen peroxide was carried out. The free radical formed during the enzymatic oxidation suffers a non-enzymatic breakdown and the overall system was analysed and characterized. The non-enzymatic breakdown of the cation radical does not occur through a disproportionation mechanism but through a more complex mechanism. The kinetic parameters of the overall system were determined for the different Pts. These experimental data may serve in the understanding of the pharmacological action of Pts.
Vázquez
A
A
Departamento de Química-Físca, E. U. del Profesorado de E.G.B. de Albacete, Universidad de Castilla-La Mancha, Espinardo, Murcia, Spain.
Tudela
J
J
Varón
R
R
García-Cánovas
F
F
D002021:Buffers; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008433:Mathematics; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry*; D010640:Phenothiazines / Q000737:chemistry* / Q000494:pharmacology; D013053:Spectrophotometry; D013881:Thioridazine / Q000737:chemistry; D014268:Trifluoperazine / Q000737:chemistry; D014273:Triflupromazine / Q000737:chemistry
D002021:Buffers; D005609:Free Radicals; D010640:Phenothiazines; D014268:Trifluoperazine; D006861:Hydrogen Peroxide; D010544:Peroxidases; D013881:Thioridazine; D014273:Triflupromazine