Apolipoprotein A-II modulates HDL remodeling in plasma.
Native and reduced-carboxamidomethylated (RCM) HDL3 are incubated with a lipoprotein-depleted plasma fraction in the presence of triacylglycerol-rich particles isolated from Intralipid. Unmodified HDL3 are mainly converted into large HDL2b particles (diameter: 9.84 +/- 0.15 nm); RCM-HDL3 are transformed into both large HDL2b (9.76 +/- 0.10 nm) and small HDL3c (7.68 +/- 0.07 nm). These findings indicate that the apo A-II dimers participate in the remodeling of HDL, by inhibiting the formation of small HDL particles.
Calabresi
L
L
Center E. Grossi Paoletti, University of Milano, Italy.
Banfi
C
C
Sirtori
C R
CR
Franceschini
G
G
D000818:Animals; D016633:Apolipoprotein A-II / Q000378:metabolism*; D005217:Fat Emulsions, Intravenous / Q000378:metabolism; D000882:Haplorhini; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood / Q000378:metabolism*; D010084:Oxidation-Reduction; D010316:Particle Size; D014280:Triglycerides / Q000378:metabolism
D016633:Apolipoprotein A-II; D005217:Fat Emulsions, Intravenous; D008075:Lipoproteins, HDL; D014280:Triglycerides