Effect of chlorpromazine on the smg GDS action.
A stimulatory GDP/GTP exchange protein for smg p21 (smg GDS) stimulated the binding of guanosine 5'-(3-0-thio) triphosphate (GTP gamma S) to smg p21B. Chlorpromazine (CPZ) inhibited the smg GDS action in a manner competitive with smg GDS and in a manner noncompetitive with smg p21B. In spite of the inhibitory effect of CPZ on the smg GDS action, it counteracted the inhibition of the smg GDS action by acidic phospholipids. These results suggest that CPZ interacts with smg p21B, smg GDS, or both, and thereby inhibits the smg GDS action, and that CPZ also interacts with the acidic phospholipids and thereby counteracts their inhibitory effect on the smg GDS action.
Kim
Y S
YS
Department of Biochemistry, Kobe University School of Medicine, Japan.
Kikuchi
A
A
Takai
Y
Y
D000639:Amitriptyline / Q000494:pharmacology; D001418:Baclofen / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002746:Chlorpromazine / Q000494:pharmacology*; D003992:Dibucaine / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D010634:Phenobarbital / Q000494:pharmacology; D010712:Phosphatidic Acids / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D020668:rap GTP-Binding Proteins
D010712:Phosphatidic Acids; D010743:Phospholipids; D006153:Guanosine Diphosphate; D000639:Amitriptyline; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D011433:Propranolol; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins; D001418:Baclofen; D003992:Dibucaine; D002746:Chlorpromazine; D010634:Phenobarbital