title: A gamma delta+ T-cell leukemia bearing a novel t(8;14)(q24;q11) translocation demonstrates spontaneous in vitro natural killer-like activity.
abstract: A highly malignant human T-cell leukemia was identified by cell surface analysis as a member of the T-cell receptor (TCR) gamma delta lineage. Cytogenetic and molecular analysis showed a novel t(8;14)(q24;q11) rearrangement involving the J delta 1 gene segment on chromosome 14 and the distal end of chromosome 8 near the c-myc proto-oncogene locus. The gamma delta TCR of the leukemia blasts was functionally intact and could be activated to generate intracellular calcium flux and to target Fc receptor-mediated redirected tumor cell lysis. In addition, non-major histocompatibility complex restricted lysis of a limited target cell panel was shown by fresh leukemic blasts and by the in vitro-maintained leukemia cells that was comparable to known T-cell lines with natural killer-like activity. These data suggest that the T-cell leukemia potentially had in vivo functional cytolytic activity. However, whether this activity did contribute to the patient's clinical condition could not be determined.
authors.affiliation: Division of Hematology, Brigham and Women's Hospital, Boston, MA.
authors.forename: R T
authors.initials: RT
authors.lastname: Maziarz
authors.forename: R J
authors.initials: RJ
authors.lastname: Arceci
authors.forename: S C
authors.initials: SC
authors.lastname: Bernstein
authors.forename: L
authors.initials: L
authors.lastname: Frazier
authors.forename: B R
authors.initials: BR
authors.lastname: Smith
authors.forename: M
authors.initials: M
authors.lastname: Kasai
authors.forename: R
authors.initials: R
authors.lastname: Tantravahi
authors.forename: J L
authors.initials: JL
authors.lastname: Strominger
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D002883:Chromosomes, Human, Pair 14; D002898:Chromosomes, Human, Pair 8; D016259:Genes, myc; D006801:Humans; D007223:Infant; D007694:Killer Cells, Natural / Q000276:immunology*; D015458:Leukemia, T-Cell / Q000235:genetics* / Q000276:immunology; D008297:Male; D000090063:Proto-Oncogene Mas; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D014178:Translocation, Genetic
chemical_list: D015703:Antigens, CD; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016692:Receptors, Antigen, T-Cell, gamma-delta