ASP-56, a new actin sequestering protein from pig platelets with homology to CAP, an adenylate cyclase-associated protein from yeast.
A new 56 kDa actin-binding protein (ASP-56) was isolated from pig platelet lysate. In falling ball viscosimetry it caused a reduction in viscosity that could be attributed to a decrease in the concentration of polymeric actin. Fluorescence measurements with NBD-labelled actin showed reduction of polymeric actin, too. These results could be explained by sequestering of actin in a non-polymerizable 1:1 ASP-56/actin complex. Sequencing of about 20 tryptic peptides of ASP-56 and comparison with known sequences revealed about 60% homology to the adenylate cyclase-associated protein (CAP) from yeast.
Gieselmann
R
R
Biophysik Department, Technische Universität München, Garching, Germany.
Mann
K
K
D051344:Actin Capping Proteins; D000199:Actins / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001792:Blood Platelets / Q000737:chemistry*; D002352:Carrier Proteins; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008840:Microfilament Proteins / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D011506:Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D051344:Actin Capping Proteins; D000199:Actins; C489097:CAP1 protein, S cerevisiae; D002352:Carrier Proteins; C034037:F-actin-binding proteins; D008840:Microfilament Proteins; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; D000262:Adenylyl Cyclases