Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae.
An unusual protein splicing reaction joins the N-terminal segment (A) and the C-terminal segment (C) of the 119K primary translation product (ABC) of the yeast VMA1 gene to yield a 69K vacuolar H(+)-ATPase subunit (AC) and an internal 50K polypeptide (B). This 50K protein is a site-specific DNA endonuclease that shares 34% identity with the homothallic switching endonuclease. The site cleaved by the VMA1-derived endonuclease exists in a VMA1 allele that lacks the derived endonuclease segment of the open reading frame. Cleavage at this site only occurs during meiosis and initiates 'homing', a genetic event that converts a VMA1 allele lacking the endonuclease coding sequence into one that contains it.
Gimble
F S
FS
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Thorner
J
J
D000483:Alleles; D001483:Base Sequence; D002872:Chromosome Deletion; D004271:DNA, Fungal / Q000737:chemistry; D003850:Deoxyribonuclease I / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005785:Gene Conversion; D005800:Genes, Fungal; D007425:Intracellular Membranes / Q000201:enzymology; D007438:Introns; D008540:Meiosis / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D006180:Proton-Translocating ATPases / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013172:Spores, Fungal / Q000502:physiology; D014169:Transformation, Bacterial; D014617:Vacuoles / Q000201:enzymology
D004271:DNA, Fungal; D011994:Recombinant Proteins; D003850:Deoxyribonuclease I; D006180:Proton-Translocating ATPases