Binding sites involved in the interaction of actin with the N-terminal region of dystrophin.
Two actin-binding sites have been identified on human dystrophin by proton NMR spectroscopy of synthetic peptides corresponding to defined regions of the polypeptide sequence. These are Actin-Binding Site 1 (ABS1) located at residues 17-26 and Actin-Binding Site 2 (ABS2) in the region of residues 128-156. Using defined fragments of the actin amino acid sequence, ABS1 has been shown to bind to actin in the region represented by residues 83-117 and ABS2 to the C-terminal region represented by residues 350-375. These dystrophin-binding sites lie on the exposed domain in the actin filament.
Levine
B A
BA
Department of Physiology, University of Birmingham, UK.
Moir
A J
AJ
Patchell
V B
VB
Perry
S V
SV
D000199:Actins / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D016189:Dystrophin / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D013049:Spectrin / Q000737:chemistry; D013329:Structure-Activity Relationship
D000199:Actins; D016189:Dystrophin; D013049:Spectrin