title: PDGF BB stimulates proliferation and differentiation in cultured chondrocytes from rat rib growth plate.
abstract: The role of two isoforms (PDGF AA and PDGF BB) of platelet derived growth factor either alone or in combination with insulin-like growth factor I, on the regulation of proliferation and differentiation of rat rib growth plate chondrocytes was analyzed. PDGF BB increased DNA-synthesis in a dose dependent manner with a half maximal effect at 1 ng/ml. When PDGF BB was combined with IGF-I, an additive effect on DNA-synthesis was observed. PDGF AA and BB alone or combined with IGF-I had no appreciable effects on proteoglycan synthesis. Both homodimers caused an increase in AP-activity, indicating stimulation of cell differentiation. Cultured chondrocytes bound 125I-PDGF AA and 125I-PDGF BB and after stimulation with PDGF expressed c-fos protein. Thus, both homodimers play an important role in chondrocyte differentiation and together with IGF-I interact in the regulation of longitudinal bone growth.
authors.affiliation: Department of Medical Cell Genetics, Medical Nobel Institute, Stockholm, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Wroblewski
authors.forename: C
authors.initials: C
authors.lastname: Edwall
mesh_terms: D000199:Actins / Q000032:analysis; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D004247:DNA / Q000096:biosynthesis; D006132:Growth Plate / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D011509:Proteoglycans / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000199:Actins; D010982:Platelet-Derived Growth Factor; D011509:Proteoglycans; D007334:Insulin-Like Growth Factor I; D004247:DNA; D000469:Alkaline Phosphatase