title: Maturational changes in airway smooth muscle structure-function relationships.
abstract: Airways become less compliant with age. When examined at either extreme of the developmental spectrum, airway smooth muscle (ASM) undergoes changes that parallel the trachea: both passive and active stress increase from preterm to adult. To determine how ASM changes throughout maturation, trachealis muscles from sheep airways of five age groups (group 1, less than 110 d gestation; group 2, 110-124 d gestation; group 3, 125-140 d gestation; group 4, newborn; and group 5, adult) were separated from their cartilaginous supports and cleaned of their mucosa and serosa. The length at which active stress was optimal was determined and passive and active stress were measured. Concentration-effect curves for acetylcholine (ACh) and KCl were performed at the length at which active stress was optimal. Morphometric analysis of the muscle was performed by computerized image analysis. At the length at which active stress was optimal, both passive and active stress increased with maturation (p less than 0.001). Concentration-effect curves for both ACh and KCl also showed a significant increase in active stress as a function of dose and of age (p less than 0.002), and the ED50 for ACh decreased with maturation (p less than 0.005). Although muscle length, thickness, and area increased with age (p less than 0.005), the ratio of contractile to connective tissue within the muscle bundle remained constant throughout maturation. These data demonstrate that ASM undergoes a progressive increase in contractility and sensitivity to ACh throughout maturation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pediatrics, St. Christopher's Hospital for Children, Temple University School of Medicine, Philadelphia, Pennsylvania 19134-1095.
authors.forename: H B
authors.initials: HB
authors.lastname: Panitch
authors.forename: K S
authors.initials: KS
authors.lastname: Deoras
authors.forename: M R
authors.initials: MR
authors.lastname: Wolfson
authors.forename: T H
authors.initials: TH
authors.lastname: Shaffer
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000403:Airway Resistance / Q000187:drug effects / Q000502:physiology; D000818:Animals; D005260:Female; D005333:Fetus / Q000033:anatomy & histology / Q000502:physiology; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D024510:Muscle Development; D009130:Muscle, Smooth / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology; D011247:Pregnancy; D012132:Respiratory Muscles / Q000033:anatomy & histology / Q000254:growth & development* / Q000502:physiology; D012756:Sheep; D014132:Trachea / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology
chemical_list: D011189:Potassium Chloride; D000109:Acetylcholine