The mRNA 5' cap-binding protein, eIF-4E, cooperates with v-myc or E1A in the transformation of primary rodent fibroblasts.
We present evidence that eIF-4E, the mRNA 5' cap-binding protein, cooperates with two immortalizing oncogenes, v-myc and E1A, to cause transformation of rat embryo fibroblasts. eIF-4E alone can transform rat embryo fibroblasts when selection is applied. The pattern of transformation by eIF-4E is similar to that of p21 Ras, raising the possibility that eIF-4E shares a common signal transduction pathway with p21 Ras.
Lazaris-Karatzas
A
A
Department of Biochemistry, McGill University, Montréal, Québec, Canada.
Sonenberg
N
N
D017456:Adenovirus Early Proteins; D000818:Animals; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D039561:Eukaryotic Initiation Factor-4E; D005347:Fibroblasts; D016259:Genes, myc; D009856:Oncogene Proteins, Viral / Q000235:genetics; D009857:Oncogenes; D010448:Peptide Initiation Factors / Q000378:metabolism*; D039381:RNA Cap-Binding Proteins; D012315:RNA Caps / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection
D017456:Adenovirus Early Proteins; D039561:Eukaryotic Initiation Factor-4E; D009856:Oncogene Proteins, Viral; D010448:Peptide Initiation Factors; D039381:RNA Cap-Binding Proteins; D012315:RNA Caps; D016601:RNA-Binding Proteins