title: Effect of changes in sodium intake on atrial natriuretic factor (ANF) and peptides derived from the N terminus of the ANF prohormone in the rat.
abstract: The purpose of the present study was to determine whether variations in salt intake would alter the plasma concentrations of atrial natriuretic factor and the N-terminal atrial natriuretic factor prohormone peptides proANF 1-98 and proANF 31-67. Two groups of rats were placed on different salt intakes for 1 week. The low salt group of rats was fed a diet providing less than 0.1 mM NaCl/day and given deionized water to drink. The normal salt group of rats was fed regular rat chow with deionized water to drink, providing them with approximately 2 mM NaCl/day. Plasma atrial natriuretic factor was 204 +/- 60 pg/ml (mean +/- SE) in normal salt rats and was significantly lower in the low salt group (44 +/- 13 pg/ml, P less than 0.01). ProANF 1-98 was also significantly higher in the normal salt group (635 +/- 47 pg/ml) compared with the low salt group (353 +/- 33 pg/ml, P less than 0.01). ProANF 31-67 was 123 +/- 21 pg/ml in the normal salt group and 59 +/- 12 pg/ml in the low salt group (P less than 0.05). Plasma renin activity in ng angiotensin l/ml/hr averaged 1.80 +/- 0.15 in the normal salt group of rats and was significantly higher in the low salt group of rats (5.66 +/- 1.07, P less than 0.05). These results suggest that atrial natriuretic factor and the atrial natriuretic factor prohormones may play a role in the physiological adjustments to low salt intake.
authors.affiliation: Department of Physiology, University of South Florida, College of Medicine, Tampa.
authors.forename: J R
authors.initials: JR
authors.lastname: Dietz
authors.forename: D L
authors.initials: DL
authors.lastname: Vesely
authors.forename: S J
authors.initials: SJ
authors.lastname: Nazian
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000502:physiology; D008297:Male; D010446:Peptide Fragments / Q000097:blood*; D011498:Protein Precursors / Q000097:blood* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism; D012982:Sodium, Dietary / Q000008:administration & dosage*
chemical_list: D010446:Peptide Fragments; D011498:Protein Precursors; D012982:Sodium, Dietary; C054548:atrial natriuretic factor prohormone (31-67); C472610:atrial natriuretic peptide, rat; C055659:atrial natriuretic factor prohormone (1-98); D009320:Atrial Natriuretic Factor; D012964:Sodium