title: Effect of local hemostatics on bone induction in rats: a comparative study of bone wax, fibrin-collagen paste, and bioerodible polyorthoester with and without gentamicin.
abstract: Local hemostatics for osseous tissue should preferably be absorbable and biocompatible and should not inhibit osteogenesis. The tissue response and effect on demineralized bone-induced heterotopic osteogenesis in the abdominal muscle of 120 male Wistar rats by different local hemostatics were evaluated by light microscopy and 85Sr uptake analyses. Non-absorbable bone wax of 88% beeswax and absorbable bovine fibrin-collagen paste both significantly inhibited osteoinduction, whereas a bioerodible polyorthoester drug delivery system with or without 4% gentamicin did not. Bone wax was not absorbed and induced a chronic foreign body reaction. Fibrin-collagen paste induced less inflammation with numerous monocytes and macrophages with engulfed material. Bioerodible polyorthoester caused a very moderate tissue reaction and was mostly resorbed at week 4.
authors.affiliation: Institute for Surgical Research, Rikshospitalet, University of Oslo, Norway.
authors.forename: E
authors.initials: E
authors.lastname: Solheim
authors.forename: E M
authors.initials: EM
authors.lastname: Pinholt
authors.forename: G
authors.initials: G
authors.lastname: Bang
authors.forename: E
authors.initials: E
authors.lastname: Sudmann
mesh_terms: D000042:Absorption; D000818:Animals; D001672:Biocompatible Materials / Q000493:pharmacokinetics / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects* / Q000473:pathology; D003094:Collagen / Q000493:pharmacokinetics / Q000494:pharmacology; D004338:Drug Combinations; D005337:Fibrin / Q000493:pharmacokinetics / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D006490:Hemostatics / Q000493:pharmacokinetics / Q000494:pharmacology*; D007249:Inflammation / Q000209:etiology / Q000473:pathology; D008297:Male; D008422:Materials Testing; D010012:Osteogenesis / Q000187:drug effects; D010168:Palmitates / Q000493:pharmacokinetics / Q000494:pharmacology; D011091:Polyesters / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014885:Waxes / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D004338:Drug Combinations; D005839:Gentamicins; D006490:Hemostatics; D010168:Palmitates; D011091:Polyesters; D014885:Waxes; C055480:bone wax; C045986:Alzamer; D005337:Fibrin; D003094:Collagen