Telomere-related markers for the pseudoautosomal region of the mouse genome.
The pseudoautosomal (PA) region of the mammalian genome is the region of the X and Y chromosomes that shares extensive DNA sequence homology and is of special interest because it may play an essential role during male meiosis. We have identified three telomere-related restriction fragments from the PA region of the mouse genome, using an oligonucleotide probe composed of the mammalian telomere consensus sequence TTAGGG. PA assignment of two C57BL/6J-derived fragments was initially suggested by analysis of DNAs from progeny sired by C57BL/6J males carrying the rearranged Y chromosome, Y*: the hybridization intensity of both fragments was concordant with the sex-chromosome complement of the offspring. Further analysis indicated that both fragments were present in female and male F1, mice regardless of the sex of their C57BL/6J parent--a criterion for autosomal or PA linkage. Both fragments were closely linked to each other and located on the X chromosome distal to amelogenin (Amg)--in agreement with X or PA linkage. Confirmation of the PA derivation of these fragments was accomplished by following their segregation in a cross involving XY* males mated to DBA/2J females. A similar experiment identified a third PA-derived restriction fragment of LT/SvEi origin. Identification of PA-derived telomere-related restriction fragments will enable further genetic analysis of this region of the mouse genome.
Eicher
E M
EM
Jackson Laboratory, Bar Harbor, ME 04609.
Lee
B K
BK
Washburn
L L
LL
Hale
D W
DW
King
T R
TR
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D006570:Heterochromatin / Q000502:physiology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D009115:Muridae / Q000235:genetics*; D015345:Oligonucleotide Probes; D011995:Recombination, Genetic; D016615:Telomere / Q000502:physiology*; D014960:X Chromosome; D014998:Y Chromosome
D015342:DNA Probes; D005819:Genetic Markers; D006570:Heterochromatin; D015345:Oligonucleotide Probes; D004247:DNA