ortho-toluidine blood protein adducts: HPLC analysis with fluorescence detection after a single dose in the adult male rat.
Hemoglobin (Hb) and albumin (Alb) adducts of the suspect human carcinogen ortho-toluidine (OT) were quantified in blood samples collected from rats after a single i.p. injection. Mild alkaline hydrolysis of Hb adducted with [14C]OT followed by extraction with ethyl acetate resulted in recovery of 63% of the bound radioactivity. HPLC analysis revealed a single radiolabeled peak which was identified as OT by GC-MS. In subsequent experiments Hb and Alb adduct levels were determined by HPLC analysis of this cleavage product using fluorescence detection. 4-Ethylaniline was used as internal standard. The detection limit for OT was approximately 450 pg/injection or 5 pmol/mg Hb. Mean adduct levels for Hb increased rapidly over the first 4 hr with the highest (ng/mg Hb +/- SD) 3.7 +/- 0.5 detected 24 hr after OT administration at 50 mg/kg body wt. In contrast, adduct levels for pooled Alb samples increased from 0.7 ng/mg Alb at 2 hr to 2.5 ng/mg Alb at 4 hr, but were not detectable 24 hr after dosing. Hb adducts showed a linear relationship for OT doses of 10, 20, 40, 50, and 100 mg/kg body wt. The Hb adduct t1/2 (11 days) was determined after a single 100 mg/kg OT dose. Hb adduct levels were quantifiable (1.3 +/- 0.2 ng/mg Hb) by HPLC/fluorescence 28 days after 100 mg/kg OT. Although Hb and Alb adducts differ in stability, a ratio of such OT adducts may be useful in long-term industrial biomonitoring for evaluation of OT exposure.
Cheever
K L
KL
Department of Health and Human Services, Centers for Disease Control, National Institute for Occupational Safety and Health, Cincinnati, Ohio 45226.
DeBord
D G
DG
Swearengin
T F
TF
Booth-Jones
A D
AD
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004333:Drug Administration Routes; D005453:Fluorescence; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism*; D014052:Toluidines / Q000097:blood*
D006454:Hemoglobins; D012709:Serum Albumin; D014052:Toluidines; C023622:2-toluidine