title: Cloning and mapping of the CYS4 gene of Saccharomyces cerevisiae.
abstract: A DNA fragment containing the CYS4 gene of Saccharomyces cerevisiae was isolated from a genomic library. The cloned fragment hybridized to the transverse-alternating-field-electrophoresis band corresponding to chromosomes VII and XV. According to the 2 microns DNA chromosome-loss procedure, the cys2 and cys4 mutations, which are linked together and co-operatively confer cysteine dependence, were assigned to chromosome VII. By further mapping involving tetrad analysis, the cys2-cys4 pair was localized between SUP77 (SUP166) and ade3 on the right arm of chromosome VII.
authors.affiliation: Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
authors.forename: B
authors.initials: B
authors.lastname: Ono
authors.forename: C
authors.initials: C
authors.lastname: Heike
authors.forename: Y
authors.initials: Y
authors.lastname: Yano
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Naito
authors.forename: S
authors.initials: S
authors.lastname: Nakagami
authors.forename: A
authors.initials: A
authors.lastname: Yamane
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003541:Cystathionine beta-Synthase / Q000235:genetics; D003545:Cysteine / Q000096:biosynthesis*; D005800:Genes, Fungal; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D051050:Serine O-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D051050:Serine O-Acetyltransferase; D003541:Cystathionine beta-Synthase; D003545:Cysteine