Fatty acids rendered immunogenic.
A method allowing the covalent binding of fatty acids to a carrier was developed. The absence of absorbed fatty acids was controlled by HPTLC. Rabbits were immunized with these immunogens. The antibodies obtained were studied by ELISA and were shown to be directed against the fatty acyl chain, whatever its length.
Maneta-Peyret
L
L
IBCN-CNRS, Bordeaux, France.
Picard
J P
JP
Bezian
J H
JH
Cassagne
C
C
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002855:Chromatography, Thin Layer; D004337:Drug Carriers; D004797:Enzyme-Linked Immunosorbent Assay; D005227:Fatty Acids / Q000737:chemistry / Q000276:immunology*; D008024:Ligands; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry
D004337:Drug Carriers; D005227:Fatty Acids; D008024:Ligands; D012710:Serum Albumin, Bovine