title: Histaminase activity in rat lung and its comparison with intestinal mucosal diamine oxidase.
abstract: In rat lung microsomes, an enzyme showing high histaminase activity is present. The oxidation of histamine is dependent on the presence of two enzymic activities, both inhibited by alpha-aminoguanidine and by B24, an inhibitor of semicarbazide-sensitive amine oxidases (SSAO) which have benzylamine as preferential substrate. These enzymic activities differ in substrate specificity: one appears to be a classical tissue bound SSAO enzyme with high affinity for benzylamine, the other a diamine oxidase (DAO) with properties that are very different from the classical DAO. This latter enzyme is not inhibition by high histamine concentrations and is more active at pH 8.5 than at pH 7.4.
authors.affiliation: Department of Preclinical and Clinical Pharmacology M. Aiazzi Mancini, University of Florence, Firenze, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Ignesti
authors.forename: G
authors.initials: G
authors.lastname: Banchelli
authors.forename: L
authors.initials: L
authors.lastname: Raimondi
authors.forename: R
authors.initials: R
authors.lastname: Pirisino
authors.forename: F
authors.initials: F
authors.lastname: Buffoni
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000201:enzymology*; D008168:Lung / Q000201:enzymology*; D008297:Male; D010084:Oxidation-Reduction; D011700:Putrescine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D013552:Swine
chemical_list: D006631:Amine Oxidase (Copper-Containing); D011700:Putrescine