Antibodies to MHC class II peptides are present in HIV-1-positive sera.
Seventy-five per cent of sera from HIV-1-infected individuals bind to the human B-lymphoma cells bearing the major histocompatibility class II molecule in enzyme-linked immunosorbent assay (ELISA). The binding is caused by the antibodies against the class II molecule present in the serum samples which prevent the interaction of murine anti-HLA.DR monoclonal antibody with B lymphoma in FACS analysis. The three highly conserved amino acid sequences in alpha- and beta-chains of the class II molecule and three homologous fragments in HIV-1 gp120 and gp41 were identified by computer search and synthesized. Using these peptides it was demonstrated that 28-48% of HIV-positive sera contain antibodies that cross-react with the peptide of HIV-1 origin and with the peptide from the class II molecule as well.
Zaitseva
M B
MB
All-Union Cancer Research Center, Academy of Medical Sciences, Moscow, USSR.
Moshnikov
S A
SA
Kozhich
A T
AT
Frolova
H A
HA
Makarova
O D
OD
Pavlikov
S P
SP
Sidorovich
I G
IG
Brondz
B B
BB
D000595:Amino Acid Sequence; D000906:Antibodies / Q000097:blood*; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015700:HIV Envelope Protein gp41 / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006681:HLA-D Antigens / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D016393:Lymphoma, B-Cell / Q000276:immunology; D008969:Molecular Sequence Data
D000906:Antibodies; D000911:Antibodies, Monoclonal; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D006681:HLA-D Antigens; D006684:HLA-DR Antigens