title: [The study of DNA fingerprint using probe directly labelled with horseradish peroxidase and enhanced chemiluminescence in forensic science application].
abstract: We describe here the technique of DNA probe directly labelled with horseradish peroxidase (HRP) and enhanced chemiluminescence (ECL) detection for determining DNA fingerprints. The patterns of DNA fingerprint were clear and with high distinction. The sensitivity of DNA fingerprint was 0.8 microgram, similar to the method of 32P labelling. The DNA fingerprints of 150 unrelated individuals were studied and showed that the probability of chance association of DNA fragments between random individuals was 3.7 x 10(-14). The method is stable, simple and quick, and can be used in individual identification and paternity test.
authors.affiliation: Institute of Forensic Science, Ministry of Public Security, Beijing.
authors.forename: J
authors.initials: J
authors.lastname: Ni
authors.forename: J
authors.initials: J
authors.lastname: Ye
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Zheng
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D015342:DNA Probes; D005554:Forensic Medicine; D006735:Horseradish Peroxidase; D006801:Humans; D008163:Luminescent Measurements
chemical_list: D015342:DNA Probes; D006735:Horseradish Peroxidase