A discordance between the human muscle NCAM sequence and those seen in other NCAM cDNA clones.
A cDNA clone, designated NC7, has been isolated from human foetal kidney that partially codes for the 140 kDa isoform of human NCAM. This clone contains a 6 bp insert that is not present in the human muscle cDNA clone lambda 4.4. This same sequence has also been found in both a cDNA clone obtained from a human Small Cell Lung Carcinoma (SCLC) line and in human genomic DNA. Furthermore, an equivalent sequence to the 6 bp region identified in the above samples is present in mouse, rat and chicken NCAM. The 6 bp insertion does not lie at a predicted intron/exon boundary as extrapolated by homology studies with the chicken and, therefore, the mechanism by which the sequence is deleted from the human muscle clone lambda 4.4 remains unclear.
Rossell
R J
RJ
ICRF, Frenchay Hospital, Bristol, UK.
Malik
A
A
Helfrich
W
W
Kemshead
J T
JT
Patel
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry / Q000235:genetics*; D004247:DNA / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000737:chemistry; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D015816:Cell Adhesion Molecules, Neuronal; D004247:DNA