Human intestinal mucin-like protein (MLP) is homologous with rat MLP in the C-terminal region, and is encoded by a gene on chromosome 11 p 15.5.
A cDNA specific for a human intestinal mucin (MLP) was amplified by PCR from cDNA of cultured human colonic adenocarcinoma cells, LS174T. The human cDNA shared high sequence homology with a corresponding rat intestinal mucin (MLP) cDNA in the 3' terminal region, and hybridized to the same mRNA (approximately 9.0 Kb) that was recognized by a probe for the MUC-2 human intestinal mucin gene. The gene encoding our human mucin peptide also mapped to chromosome 11 p 15.5, the known locus of MUC-2. Our findings suggest that human MLP and MUC-2 are encoded by the same gene and that rat and human intestinal mucin share a common C-terminal amino acid structure.
Xu
G
G
Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
Huan
L
L
Khatri
I
I
Sajjan
U S
US
McCool
D
D
Wang
D
D
Jones
C
C
Forstner
G
G
Forstner
J
J
D000230:Adenocarcinoma / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11; D003110:Colonic Neoplasms / Q000235:genetics*; D004252:DNA Mutational Analysis; D006801:Humans; D007421:Intestine, Small / Q000737:chemistry; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D009077:Mucins