An appraisal of different methods for the detection of the walnut strain of cherry leafroll virus.
Three methods were evaluated for the detection of cherry leafroll virus: ELISA, dot-blot and reverse transcriptional polymerase chain reaction (RT-PCR). Dot-blot and RT-PCR were carried out in crude plant extracts without any further RNA purification. Dot-blot hybridization using a 32P-labelled DNA probe was as sensitive as previously reported ELISA results for cherry leafroll virus detection. The most sensitive method was RT-PCR, which amplified a specific fragment of 448 bp from the 3' untranslated region of both viral genomic RNAs. RT-PCR was used to detect cherry leafroll virus in infected walnut buds and twigs.
Borja
M J
MJ
CIT-INIA, Dpto. Protección Vegetal, Crta. La Coruña, Madrid, Spain.
Ponz
F
F
D001483:Base Sequence; D004279:DNA, Viral; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012328:RNA Viruses / Q000302:isolation & purification; D015183:Restriction Mapping
D004279:DNA, Viral