title: Selection of mouse cells with amplified metallothionein genes retaining their glucocorticoid inducibility.
abstract: Two new mouse cell mutants, resistant to either 80 or 100 mM CdCl2, were isolated to study the regulation of transcription by the glucocorticoid hormones. Their metallothionein mt-1% and mt-2+ genes were amplified coordinately to a maximum of 30 copies per cell. By Southern blot analysis, no gross rearrangement was detectable near the mt+ loci. Contrary to other mutants previously isolated, the metallothionein-specific mRNAs of these mutants are inducible by dexamethasone.
authors.affiliation: Department de Microbiologie, Faculté de Médecine, Université de Sherbrooke, Qué, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Thibodeau
authors.forename: C
authors.initials: C
authors.lastname: De Souza
authors.forename: M
authors.initials: M
authors.lastname: Smorawinska
authors.forename: J P
authors.initials: JP
authors.lastname: Thirion
mesh_terms: D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D015139:Blotting, Southern; D002104:Cadmium / Q000494:pharmacology; D002478:Cells, Cultured; D004247:DNA; D003907:Dexamethasone / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005784:Gene Amplification; D015870:Gene Expression / Q000187:drug effects; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D002104:Cadmium; D003907:Dexamethasone; D004247:DNA; D008668:Metallothionein