8-Azido-ATP labelling of the FecE protein of the Escherichia coli iron citrate transport system.
The iron(III) transport system via citrate displays the characteristics of a binding protein-dependent transport mechanism through the cytoplasmic membrane. One of the five transport proteins, FecE, contains the two motifs found in ATP-binding proteins. Cloned overexpressed FecE was photoaffinity-labelled with [32P]8-azido-ATP. Labelling was inhibited by 1 mM ATP, ADP, GTP and less by CTP, demonstrating the specificity of the reaction. It is suggested that ATP is the principal energy source for iron(III) citrate transport through the cytoplasmic membrane of Escherichia coli.
Schultz-Hauser
G
G
Mikrobiologie II, Universität Tübingen, FRG.
Van Hove
B
B
Braun
V
V
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism*; D000345:Affinity Labels; D001386:Azides; D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002951:Citrates / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism; D011485:Protein Binding
D000345:Affinity Labels; D001386:Azides; D001426:Bacterial Proteins; D002352:Carrier Proteins; D002951:Citrates; D009711:Nucleotides; C017040:8-azidoadenosine 5'-triphosphate; D000255:Adenosine Triphosphate; D007501:Iron