Expression of mumps virus glycoproteins in mammalian cells from cloned cDNAs: both F and HN proteins are required for cell fusion.
Two recombinant plasmids were constructed by inserting the cDNAs of either the fusion (F) or the hemagglutinin-neuraminidase (HN) protein genes of mumps virus into the pcDL-SR alpha expression vector. Both the F and the HN proteins expressed in COS7 cells transfected with their respective recombinant plasmids were indistinguishable in terms of electrophoretic mobility from their counterparts synthesized in mumps virus-infected cells. The F protein was cleaved and expressed on the cell surface, but uncleaved forms were also detected. The expressed HN protein was transported to the cell surface and adsorbed guinea pig erythrocytes. Syncytium formation was induced when COS7 cells were transfected with both recombinant plasmid DNAs together, but not with the recombinant plasmid only carrying the F gene. This observation indicates that cell fusion mediated by mumps virus requires both the F and the HN glycoproteins.
Tanabayashi
K
K
Department of Measles Virus, National Institute of Health, Tokyo, Japan.
Takeuchi
K
K
Okazaki
K
K
Hishiyama
M
M
Yamada
A
A
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002522:Chlorocebus aethiops; D004247:DNA / Q000235:genetics; D015707:HN Protein / Q000502:physiology*; D066298:In Vitro Techniques; D009109:Mumps virus / Q000235:genetics*; D011994:Recombinant Proteins / Q000502:physiology; D014162:Transfection; D014760:Viral Fusion Proteins / Q000502:physiology*
D015707:HN Protein; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins; D004247:DNA