Truncated variants of gp120 bind CD4 with high affinity and suggest a minimum CD4 binding region.
The envelope glycoprotein, gp120, of human immunodeficiency virus type 1 (HIV-1) binds the cellular protein CD4 with high affinity. By deletion we show that 62 N- and 20 C-terminal residues along with the V1, V2 and V3 variable regions of gp 120 are unnecessary for CD4 binding. A 287 residue variant (ENV59), missing those 197 amino acids, binds to CD4 with high affinity. A polyclonal antibody failed to efficiently precipitate ENV59 which is consistent with the loss of immunodominant antigenic structures in the regions deleted. This suggests that ENV59 may have potential as an immunogen, able to elicit antibodies against more conserved regions of gp120. Additionally, complementing co-expressed gp120 fragments as well as a circularly permuted molecule bind CD4, and suggest either that the molecular termini are adjacent in the folded structure, or that an N-terminal region folds into the structure unconstrained by its method of attachment to the rest of the molecule.
Pollard
S R
SR
Biogen Inc., Cambridge, MA.
Rosa
M D
MD
Rosa
J J
JJ
Wiley
D C
DC
D000818:Animals; D001665:Binding Sites; D015704:CD4 Antigens / Q000378:metabolism*; D002460:Cell Line; D002872:Chromosome Deletion; D004220:Disulfides / Q000378:metabolism; D014644:Genetic Variation; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008961:Models, Structural; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D015202:Protein Engineering; D014162:Transfection
D015704:CD4 Antigens; D004220:Disulfides; D015699:HIV Envelope Protein gp120