title: [Assistance in human oocyte fertilization using micromanipulation].
abstract: In recent years different types of micromanipulation have become available to improve human gametes interaction, particularly in cases of severe sperm defect. Direct injection of one spermatozoon into the cytoplasm of the egg has not been applied in man, not only because of technical difficulties and low efficacy, but also in view of the theoretical risk of chromosomal anomalies. However, subzonal sperm injection (SUZI) and partial zona dissection (PZD) are now used routinely by a number of IVF centers. In our hands this latter method has increased significantly the fertilization rate (21.6 vs 2.9 p.cent among intact oocytes) and has allowed to obtain 3 pregnancies (of which 2 ongoing) after 28 attempts. Our results and those of the literature are discussed with respect to the risks and advantages linked to these techniques.
authors.affiliation: Unité de Recherche en Reproduction humaine, Hôpital St-Pierre, U.L.B., Bruxelles.
authors.forename: P
authors.initials: P
authors.lastname: Barlow
authors.forename: P
authors.initials: P
authors.lastname: Van der Zwalmen
authors.forename: A
authors.initials: A
authors.lastname: Delvigne
authors.forename: J
authors.initials: J
authors.lastname: Van Hoeck
authors.forename: R
authors.initials: R
authors.lastname: Schoysman
authors.forename: F
authors.initials: F
authors.lastname: Leroy
mesh_terms: D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D008845:Microinjections; D008846:Micromanipulation / Q000379:methods*; D008866:Microsurgery; D009865:Oocytes; D011247:Pregnancy; D013084:Sperm-Ovum Interactions; D015044:Zona Pellucida
vernacular_title: L'assistance à la fécondation de l'ovocyte humain par micromanipulation.