Acidic phospholipids are required during solubilization of amino acid transport systems of Lactococcus lactis.
The branched-chain amino acid transport system of Lactococcus lactis was solubilized with n-octyl beta-D-gluco-pyranoside and reconstituted into proteoliposomes. Transport activity was recovered only when solubilization was performed in the presence of acidic phospholipids. Omission of acidic phospholipids during solubilization resulted in an inactive transport protein and the activity could not be restored in the reconstitution step. Similar results have been obtained for the arginine/ornithine exchange protein from Pseudomonas aeruginosa and L. lactis. Functional reconstitution of the transport protein requires the presence of aminophospholipids or glycolipids in the liposomes (Driessen, A.J.M., Zheng, T., In't Veld, G., Op den Kamp, J.A.F. and Konings, W.N. (1988) Biochemistry 27, 865-872). We propose that during the detergent solubilization the acidic phospholipids protect the transport systems against denaturation by preventing delipidation.
In 't Veld
G
G
Department of Microbiology, University of Groningen, Haren, Netherlands.
de Vrije
T
T
Driessen
A J
AJ
Konings
W N
WN
D000597:Amino Acids, Branched-Chain / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism; D001692:Biological Transport; D006863:Hydrogen-Ion Concentration; D013294:Lactococcus lactis / Q000378:metabolism*; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009952:Ornithine / Q000378:metabolism; D010713:Phosphatidylcholines / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism*; D011510:Proteolipids; D012995:Solubility
D000597:Amino Acids, Branched-Chain; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D010743:Phospholipids; D011510:Proteolipids; C020485:proteoliposomes; D001120:Arginine; D009952:Ornithine; C017251:1,2-oleoylphosphatidylcholine