Characterization of a polyclonal anti-peptide antibody to the angiotensin II type-1 (AT1) receptor.
A polyclonal antibody has been prepared against a synthetic peptide corresponding to amino acids 14-23 of the angiotensin II type-1 (AT1) receptor. The antibody is of high titer and mono-specific. Western blot analysis of membranes from rat liver, kidney, and adrenal gland showed that the antibody specifically recognizes a protein band of MW 70,000 whose amounts are highest in the liver, followed by kidney and adrenals. In addition, a relatively less prominent band of MW 95,000 was also detected. The relative distribution of this protein correlates well with the values obtained for [3H]-DuP753 binding and AT1 receptor mRNA.
Zelezna
B
B
Department of Physiology, College of Medicine, University of Florida, Gainesville 32610.
Richards
E M
EM
Tang
W
W
Lu
D
D
Sumners
C
C
Raizada
M K
MK
D000311:Adrenal Glands / Q000737:chemistry; D000804:Angiotensin II / Q000378:metabolism*; D000910:Antibodies, Heterophile; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008566:Membranes / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis* / Q000276:immunology*; D011945:Receptors, Angiotensin / Q000032:analysis* / Q000276:immunology*
D000910:Antibodies, Heterophile; D010446:Peptide Fragments; D011945:Receptors, Angiotensin; D000804:Angiotensin II