IL-4 inhibits LPS-, IL-1 beta- and TNF alpha-induced expression of tissue factor in endothelial cells and monocytes.
Inflammatory mediators such as endotoxin, interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) dose-dependently increased the expression of tissue factor on the surface of cultured bovine aortic endothelial cells (ABAE), human umbilical vein endothelial cells (HUVEC) and human monocytes. In ABAE, endotoxin-, IL-1 beta- and TNF alpha-induced tissue factor expression was suppressed by interleukin-4 (IL-4) which also neutralized the pyrogenic effect of endotoxin in HUVEC and monocytes. IL-4 did not alter TNF-alpha-induced procoagulant changes in HUVEC and monocytes but strongly protected the monocyte surface against IL-1 beta-induced procoagulant changes.
Herbert
J M
JM
Sanofi Recherche, Toulouse, France.
Savi
P
P
Laplace
M C
MC
Lale
A
A
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors; D015847:Interleukin-4 / Q000494:pharmacology*; D008070:Lipopolysaccharides; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D013925:Thromboplastin / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors
D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D013925:Thromboplastin