Interaction of calmodulin with lactoferrin.
Calmodulin, as a major intracellular calcium-binding protein, regulates many Ca(2+)-dependent enzymes and plays an important role in a wide spectrum of cellular functions of the eukaryotes. Interaction between calmodulin and human lactoferrin, a 78 kDa protein with antibacterial properties, was found in the presence of Ca2+ using (i) a method for the detection of calmodulin binding proteins with biotinylated calmodulin, (ii) affinity chromatography on an agarose-calmodulin column with subsequent detection by an enzyme-linked immunosorbent assay (ELISA). The binding of calmodulin to lactoferrin blocked the ability of lactoferrin to agglutinate Micrococcus lysodeikticus.
de Lillo
A
A
Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
Tejerina
J M
JM
Fierro
J F
JF
D000372:Agglutination Tests; D000818:Animals; D001710:Biotin; D002147:Calmodulin / Q000378:metabolism*; D002645:Chickens; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D007781:Lactoferrin / Q000378:metabolism*; D008837:Micrococcus / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism
D002147:Calmodulin; D001710:Biotin; D009113:Muramidase; D007781:Lactoferrin