In vivo resolution of circular plasmids containing concatemer junction fragments from minute virus of mice DNA and their subsequent replication as linear molecules.
During replication of their linear, single-stranded DNA genomes, parvoviruses generate a series of concatemeric duplex intermediates. We have cloned, into Escherichia coli plasmids, junction fragments from these palindromic concatemers of minute virus of mice DNA spanning both the right end-to-right end (viral 5' to 5') and left end-to-left end (viral 3' to 3') fusions. When mouse cells were transfected with these circular plasmids and superinfected with minute virus of mice, the viral junctions were resolved and the plasmids replicated as linear chromosomes with vector DNA in their centers and viral DNA at their termini. Resolution did not occur when the concatemer joint was replaced by a different palindromic sequence or when the transfected cells were not superinfected, indicating the presence of latent origins of replication which could only be activated by a viral trans-acting factor(s). Moreover, the products of resolution and replication from the two termini were characteristically different. Analysis of individual terminal fragments showed that viral 5' (right-end) sequences were resolved predominantly into "extended" structures with covalently associated copies of the virally encoded NS-1 polypeptide, while bridges derived from the 3' (left) end resolved into both NS-1-associated extended termini and lower-molecular-weight "turn-around" forms in which the two DNA strands were covalently continuous. This pattern of resolution exactly coincides with that seen at the two termini of replicative-form intermediates in normal virus infections. These results demonstrate that the bridge structures are authentic substrates for resolution and indicate that the frequency with which extended versus turn-around forms of each terminus are generated is an intrinsic property of the telomere.
Cotmore
S F
SF
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
Tattersall
P
P
D000818:Animals; D015139:Blotting, Southern; D002213:Capsid / Q000378:metabolism; D003001:Cloning, Molecular; D004261:DNA Replication; D004270:DNA, Circular / Q000096:biosynthesis*; D004279:DNA, Viral / Q000096:biosynthesis; D007739:L Cells; D051379:Mice; D008915:Minute Virus of Mice / Q000235:genetics*; D010957:Plasmids; D014758:Viral Core Proteins / Q000378:metabolism; D017361:Viral Nonstructural Proteins
D004270:DNA, Circular; D004279:DNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins