title: Eicosapentaenoic acid inhibits antigen-presenting cell function of murine splenocytes.
abstract: Recently, many investigators have studied the effects of eicosapentaenoic acid (EPA)-rich fish oil on immune function and immune disease. However, effects of dietary supplementation of fish oil or EPA on the immune system are still unclear. In the present study, the effects of EPA on antigen presentation were investigated. We have used antigen-specific helper T-cell clones that proliferate in the presence of antigen [keyhole limpet haemocyanin (KLH)] and spleen cells as antigen-presenting cells (APC). Mice were divided into two groups and fed an experimental diet or a control diet for 4 weeks ad libitum. In mice fed the experimental diet, the arachidonic acid (AA) content of spleen cells was decreased and that of EPA and docosapentaenoic acid was increased markedly compared to those of the control diet. Dietary enrichment with EPA inhibited the ability of accessory cells to present antigen to murine helper T-cell clones. This effect was observed for two distinct helper T-cell clones, Th1 and Th2. We also examined the effects of EPA-TG emulsion on APC function. The direct addition of EPA-TG emulsion to a T-cell proliferation assay system suppressed APC function. The inhibition was proportional to the concentration of EPA-TG emulsion. Pretreatment of splenocytes with EPA-TG emulsion resulted in inhibition of APC function. Inhibition of antigen presentation by dietary supplementation with EPA might depress immune reactivity.
authors.affiliation: First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Fujikawa
authors.forename: N
authors.initials: N
authors.lastname: Yamashita
authors.forename: K
authors.initials: K
authors.lastname: Yamazaki
authors.forename: E
authors.initials: E
authors.lastname: Sugiyama
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Hamazaki
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000187:drug effects*; D004032:Diet; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013025:Glycine max; D013154:Spleen / Q000737:chemistry / Q000276:immunology*; D014280:Triglycerides / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D014280:Triglycerides; C051339:1,2,3-trieicosapentaenoyl glycerol; D015118:Eicosapentaenoic Acid