title: Sequence analysis and editing of the phosphoprotein (P) gene of rinderpest virus.
abstract: We have cloned the cDNA of the phosphoprotein (P) gene of the virulent (Kabete "O") strain of rinderpest virus and provided a comparative analysis of its sequence with that of the P genes of measles, canine distemper, and phocid distemper viruses. The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids with a calculated molecular weight of 54,344. The second ATG would produce a C polypeptide of 177 residues with a predicted molecular weight of 19,927. In addition, the insertion of a G residue at position 740 generates an alternative mRNA potentially encoding the V polypeptide of rinderpest virus. The homology comparisons in P amino acid sequences between rinderpest and measles, between rinderpest and canine distemper, and between rinderpest and phocid distemper viruses are 60, 44, and 46%, respectively. A four-way comparison shows an identity of 34%. Similar homology comparisons with the C amino acid sequence between rinderpest and measles, rinderpest and canine distemper, and rinderpest and phocid distemper viruses are 56, 42, and 40%, respectively. A homology of 31% is found in a four-way comparison for the C polypeptide. From the point of the insertion of the G residue, there is a homology of 78% between the V polypeptides of rinderpest and measle viruses.
authors.affiliation: Department of Veterinary Microbiology and Immunology, University of California, Davis 95616.
authors.forename: M
authors.initials: M
authors.lastname: Yamanaka
authors.forename: B
authors.initials: B
authors.lastname: Dale
authors.forename: T
authors.initials: T
authors.lastname: Crisp
authors.forename: B
authors.initials: B
authors.lastname: Cordell
authors.forename: M
authors.initials: M
authors.lastname: Grubman
authors.forename: T
authors.initials: T
authors.lastname: Yilma
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D012302:Rinderpest virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D010750:Phosphoproteins; D014764:Viral Proteins; C076637:P gene protein, Rinderpest virus