Changes in rabbit corpus luteum progesterone secretion and cellular morphology following unilateral luteectomy or ovariectomy.
The objective of this study was to determine whether removal of corpora lutea (CL) from one ovary (unilateral luteectomy; ULL) or removal of the entire ovary (unilateral ovariectomy; ULO) of pseudopregnant rabbits would cause compensatory growth and progesterone production by the contralateral ovary. Pseudopregnancy was induced in rabbits with hCG (Day 0). On the first day of pseudopregnancy, one group of rabbits received a sham operation (controls), another group underwent ULL, and a third group underwent ULO. On Day 11 of pseudopregnancy, each rabbit underwent laparotomy, the ovarian artery and vein were cannulated, and the ovary(ies) was removed and perfused in vitro for 6 h. The mean CL weight increased by 33% in the ULL group and by 28% in the ULO group as compared to sham-operated controls. Peripheral estradiol and progesterone levels in sham-operated control, ULL, and ULO groups were similar. Ovarian venous estradiol levels were similar in the control and ULL groups, but were significantly increased in the remaining ovary of the ULO group. Both ovarian venous progesterone in vivo and progesterone secretion in vitro increased significantly in contralateral ovaries from ULL and ULO rabbits as compared to sham-operated controls. Progesterone secretion by ovaries perfused in vitro increased significantly in the contralateral ovary of the ULL and ULO groups. Mean number of luteal cells per CL increased significantly in the ULL group, but not in the ULO group. In contrast, luteal cell volume increased significantly in the ULO, but not in the ULL group. The stimuli responsible for increased progesterone production following ULL and ULO result in morphological changes in the remaining CL.(ABSTRACT TRUNCATED AT 250 WORDS)
Dharmarajan
A M
AM
Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Zanagnolo
V L
VL
Dasko
L M
LM
Hardy
M P
MP
Wallach
E E
EE
D000818:Animals; D003338:Corpus Luteum / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D004958:Estradiol / Q000097:blood; D005260:Female; D066298:In Vitro Techniques; D008184:Luteal Cells / Q000166:cytology; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology; D011374:Progesterone / Q000097:blood / Q000378:metabolism*; D011555:Pseudopregnancy / Q000473:pathology / Q000503:physiopathology; D011817:Rabbits
D011374:Progesterone; D004958:Estradiol