Appearance of thymosin alpha 1 in supernatants of monocytes incubated with prothymosin alpha.
Prothymosin alpha, a polypeptide of 109 to 111 amino acid residues, contains the entire thymosin alpha 1 sequence (residues 1-28) at its amino terminal. Human peripheral blood monocytes incubated with prothymosin alpha release thymosin alpha 1 in the culture supernatants. In addition total RNA is found to increase. The production of thymosin alpha 1 involves de novo protein synthesis as shown by the kinetics of this release and its inhibition by actinomycin D and cycloheximide. Thymosin alpha 1 release, possibly in association with HLA-DR, stimulates the proliferation of the T cell population.
Frillingos
S
S
Laboratory of Biological Chemistry, University of Ioannina Medical School, Greece.
Seferiadis
K
K
Papanastasiou
M
M
Baxevanis
C N
CN
Frangou-Lazaridis
M
M
Economou
M
M
Papamichail
M
M
Tsolas
O
O
D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007700:Kinetics; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine; D013601:T-Lymphocytes / Q000378:metabolism; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D013997:Time Factors
D011498:Protein Precursors; D012333:RNA, Messenger; C040782:prothymosin alpha; D013947:Thymosin; D000077596:Thymalfasin