[Thymidine phosphorylase activity and its relationship to trimethoprim in initial strains and thymidine-, thymine-dependent and trimethoprim-resistant mutants of plague microbe].
The comparative study revealed thymidine phosphorylase activity in the initial strains of a plague microbe of the field variety and in thymidine-, thymine-dependent and trimethoprim-resistant mutants of the plague microbe of other varieties. The data fully conformed to the results of the microbiological investigation of the strains' ability to grow on the nutrient media with trimethoprim in the presence of thymine and thymidine. On the basis of these results it appeared possible to divide the initial and mutant strains of the plague microbe into four arbitrary groups: initial strains of the plague microbe of all the varieties except the field ones sensitive to trimethoprim under any temperature conditions of incubation on any medium with any supplements; initial strains of the plague microbe of the field variety resistant to trimethoprim at 28 degrees C in the presence of thymine or thymidine alone; Tmpr mutants whose resistance to trimethoprim at 28 degrees C did not depend on the presence of thymine or thymidine, purine and vitamins, but depended on the presence of these substances at a temperature of 37 degrees C.
Kravchenko
A N
AN
Mishan'kin
B N
BN
D009154:Mutation / Q000235:genetics; D013936:Thymidine / Q000378:metabolism*; D013937:Thymidine Kinase / Q000378:metabolism*; D013939:Thymidine Phosphorylase / Q000378:metabolism*; D013941:Thymine / Q000378:metabolism*; D014296:Trimethoprim Resistance / Q000235:genetics*; D015010:Yersinia pestis / Q000187:drug effects* / Q000235:genetics
D013939:Thymidine Phosphorylase; D013937:Thymidine Kinase; D013941:Thymine; D013936:Thymidine
Aktivnost' timidinfosforilazy i otnoshenie k trimetoprimu u iskhodnykh shtammov, timidino-, timinozavisimykh i trimetoprimoustoĭchivykh mutantov chumnogo mikroba.