Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils.
A novel 28 kDa protein, which we have named 'grancalcin', has been identified in human neutrophils. The protein was isolated from the cytosol and found to be a homodimer, with an apparent molecular mass of 55 kDa on gel filtration. Polyclonal antibodies were raised to the native protein. N-Terminal sequence analysis and tryptic-peptide sequence analysis was performed. The protein exhibits sequence similarity to sorcin, a 24 kDa calcium-binding protein over-expressed in certain multi-drug-resistant cell lines. It appears to be a member of the EF-hand family of calcium-binding proteins. The association of a high proportion of this protein with the membranes and granules in the presence of physiological concentrations of calcium may indicate a role in granule-membrane fusion and degranulation.
Teahan
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CG
Department of Medicine, University College London, Rayne Institute, Faculty of Clinical Sciences, London, U.K.
Totty
N F
NF
Segal
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AW
D000595:Amino Acid Sequence; D001345:Autoradiography; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000737:chemistry*; D010449:Peptide Mapping; D011499:Protein Processing, Post-Translational; D012689:Sequence Homology, Nucleic Acid
D002135:Calcium-Binding Proteins; C503121:GCA protein, human; D002118:Calcium