Deoxynucleotide pools and DNA synthesis in resting and PHA-stimulated human lymphocytes treated with mutagens.
In resting and PHA-stimulated PBL treated with uv light or MMS we measured the sizes of the dTTP and dATP pools and the variation of ATP content taken as an indicator of cytotoxicity. The effects on DNA synthesis were examined by measuring DNA repair (unscheduled DNA synthesis) in resting PBL and the inhibition of DNA replication in stimulated PBL. While both treatments affected DNA synthesis, only MMS perturbed dNTP pools and decreased the intracellular concentration of ATP. All the effects were more evident in cycling than in resting lymphocytes.
Ferraro
P
P
Department of Biology, University of Padova, Italy.
Bianchi
V
V
Biasin
M R
MR
Celotti
L
L
D000255:Adenosine Triphosphate / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000096:biosynthesis*; D004260:DNA Repair; D003854:Deoxyribonucleotides / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D010835:Phytohemagglutinins
D003854:Deoxyribonucleotides; D010835:Phytohemagglutinins; D000255:Adenosine Triphosphate; D004247:DNA; D008741:Methyl Methanesulfonate