Effect of cisplatin treatment of human monocyte cell line U937 on the induction of tumoricidal activity.
U937, a human monocyte-like, cell line was checked for cytotoxic activity against tumor target cells. Untreated U937 cells showed little cytotoxicity against tumor cells. Granulocyte-macrophage colony stimulating factor (GM-CSF) and LPS significantly activated the U937 cells to tumoricidal state. Treatment of U937 cells with cisplatin did not enhance the tumoricidal activity. Similarly, interferon gamma (IFN-Y) and macrophage colony stimulating factor (M-CSF) could also not activate either the tumoricidal activity of U937 cells. Pretreatment of U937 cells with GM-CSF for 24 h and then the treatment with cisplatin significantly augmented the tumoricidal activity as compared to that of GM-CSF alone.
Singh
R K
RK
School of Biotechnology, Banaras Hindu University, Varanasi, India.
Singh
S M
SM
Sodhi
A
A
D002945:Cisplatin / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D016260:Monocytes, Activated Killer / Q000276:immunology; D014407:Tumor Cells, Cultured
D008070:Lipopolysaccharides; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D002945:Cisplatin