title: A short form of the tick-borne encephalitis virus NS3 protein.
abstract: Using monoclonal antibodies to the tick-borne encephalitis virus (TBE) nonstructural protein NS3 two forms of this protein were revealed in TBE-infected mammalian cells: a full-length form (69 kDa) and a short form (49 kDa) which has not been observed before and was called NS3'. Recombinant plasmids were constructed and various fragments of the TBE NS3 gene were expressed in rabbit reticulocyte lysate. By analyzing immune precipitates of 35S-labeled translation products, we could monitor and localize internal cleavage of NS3, due to which the NS3' protein was generated.
authors.affiliation: Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
authors.forename: K V
authors.initials: KV
authors.lastname: Pugachev
authors.lastname: Nomokonova NYu
authors.forename: O V
authors.initials: OV
authors.lastname: Morozova
authors.forename: A G
authors.initials: AG
authors.lastname: Pletnev
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004669:Encephalitis Viruses, Tick-Borne / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D014176:Protein Biosynthesis; D020365:RNA Helicases; D012333:RNA, Messenger / Q000235:genetics; D012697:Serine Endopeptidases; D013552:Swine; D014158:Transcription, Genetic; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000235:genetics*; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D004279:DNA, Viral; D020365:RNA Helicases; D012333:RNA, Messenger; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases