Expression of interferon-gamma receptors on bladder cancer cells: does it correlate with biological response?
Previously we have shown a differential biological response of three human bladder cancer cell lines (RT4, RT112 and MGH-U1) to gamma interferon (IFN-gamma). The present study examines the relationship between the biological response and the expression of the interferon-gamma receptor on the tumour cell surface. Using a competitive radioligand binding assay and Scatchard analysis, we measured the number and affinity of the IFN-gamma receptors on each of the above cell lines. Individual cells from each line expressed large numbers (29,100-41,800) of high-affinity receptors (kd = 2.4-3.9 x 10(10) M). There was no statistically significant difference in either of these parameters between the three lines. We therefore conclude that the biological response of these bladder lines to IFN-gamma does not relate to the number or affinity of its receptor on the plasma membrane of these tumour cells.
Hawkyard
S J
SJ
Department of Surgery/Urology, Western General Hospital, UK.
Jackson
A M
AM
Hawkins
R A
RA
James
K
K
Smyth
J F
JF
Chisholm
G D
GD
D002295:Carcinoma, Transitional Cell / Q000276:immunology* / Q000628:therapy; D002460:Cell Line; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism* / Q000627:therapeutic use; D007700:Kinetics; D011971:Receptors, Immunologic / Q000378:metabolism*; D017471:Receptors, Interferon; D014407:Tumor Cells, Cultured / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology* / Q000628:therapy
D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D007371:Interferon-gamma