Association of oriC region of Escherichia coli chromosome with outer membrane: effects of culture condition.
We isolated complexes containing oriC region DNA and outer membrane, named origin complex heavy and origin complex light, from the cells of Escherichia coli cultured in media with poor or rich of nutrients, and found the different nature of association between origin DNA and outer membrane. The ratio of origin complex light to origin complex heavy prepared from the cells cultured in rich media was lower than that of those from minimal medium culture. Outer membrane preparations from the cells grown in nutritious media had high abilities of association with origin complex light in the presence of magnesium. These results indicated that the number of binding sites on outer membrane with origin region DNA increase, or the binding between outer membrane and origin region DNA become more rigid, when cells grow faster and DNA replication initiate more frequently in a nutritious medium.
Kambe-Honjoh
H
H
Department of Agricultural Chemistry, University of Tokyo, Japan.
Tamura
G
G
Nagai
K
K
D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D008274:Magnesium / Q000494:pharmacology
D003470:Culture Media; D004269:DNA, Bacterial; D008274:Magnesium