The distribution of o,p'-DDD (mitotane) among serum lipoproteins in normo- and hypertriglyceridemia.
We found that the distribution of the lipophilic chemotherapeutic agent o,p'-DDD (mitotane) among serum (lipo)proteins was altered in hypertriglyceridemia, with relatively more o,p'-DDD accumulating in the chylomicron and very-low-density lipoprotein (VLDL) fraction. Intralipid, an artificial chylomicron emulsion, or isolated VLDL could extract o,p'-DDD from the other serum (lipo)proteins. There was an inverse relationship between the relative amount of o,p'-DDD found in the fraction exhibiting a density of less than 1.006 g/ml (chylomicrons plus VLDL) and the relative amount observed in the LDL or HDL fractions of serum. Our results indicate that hypertriglyceridemia may impede the entry of o,p'-DDD into the brain or the adrenals. For therapeutic monitoring of o,p'-DDD levels in severe hypertriglyceridemia, we recommend that the chylomicron and VLDL fraction first be removed from the serum by ultracentrifugation.
Gebhardt
D O
DO
Department of Clinical Chemistry, University Hospital, Leiden, The Netherlands.
Moolenaar
A J
AJ
van Seters
A P
AP
van der Velde
E A
EA
Gevers Leuven
J A
JA
D000306:Adrenal Cortex Neoplasms / Q000097:blood / Q000188:drug therapy; D002277:Carcinoma / Q000097:blood / Q000188:drug therapy; D002849:Chromatography, Gas; D002914:Chylomicrons / Q000097:blood; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood*; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D008939:Mitotane / Q000097:blood* / Q000627:therapeutic use; D012016:Reference Values; D013997:Time Factors
D002914:Chylomicrons; D008074:Lipoproteins; D008939:Mitotane