Effects of caffeine on paracetamol activation in rat and mouse liver microsomes.
1. The effects of caffeine on the NADPH-dependent production of paracetamol-glutathione conjugate were studied in rat and mouse liver microsomes. 2. In the presence of caffeine, glutathione conjugate production in rat microsomes was enhanced, whereas that in mouse microsomes was not affected significantly, showing an apparent species difference. 3. The data partly explain the species difference in the effects of caffeine on paracetamol hepatotoxicity.
Liu
J
J
Division of Health Science, Tokyo Medical and Dental University, Japan.
Sato
C
C
Shigesawa
T
T
Kamiyama
T
T
Tajiri
K
K
Miyakawa
H
H
Marumo
F
F
D000082:Acetaminophen / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics*; D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D008658:Inactivation, Metabolic; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000082:Acetaminophen; D002110:Caffeine; D009249:NADP; C034780:3-(glutathion-S-yl)acetaminophen