Teicoplanin reduces in-vitro reactivity and murine lethality of Salmonella minnesota R595 lipopolysaccharide.
Three different tests were performed to investigate the effect of teicoplanin on lipopolysaccharide (LPS). After incubation for 3 h with teicoplanin, LPS from Salmonella minnesota R595 showed reduced reactivity in the metachromatic dimethyl-methylene blue assay and the limulus amoebocyte lysate test. In addition, galactosamine-sensitized mice had an increased survival rate, from 29% to 72%, when teicoplanin was pre-incubated for 3 h with the LPS to be injected intraperitoneally. The results suggest that teicoplanin may have a neutralizing effect on LPS.
Focà
A
A
Institute of Microbiology, University of Reggio Calabria, Policlinico Mater Domini, Catanzaro, Italy.
Matera
G
G
Berlinghieri
M C
MC
Liberto
M C
MC
De Sarro
G B
GB
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D005688:Galactosamine / Q000494:pharmacology; D006020:Glycopeptides / Q000494:pharmacology; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D008751:Methylene Blue / Q000031:analogs & derivatives; D051379:Mice; D008810:Mice, Inbred C57BL; D011112:Polymyxin B / Q000494:pharmacology; D012475:Salmonella / Q000187:drug effects*; D017334:Teicoplanin
D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D008070:Lipopolysaccharides; D017334:Teicoplanin; D005688:Galactosamine; D011112:Polymyxin B; D008751:Methylene Blue; C016401:1,9-dimethylmethylene blue