Cloning and mapping of the CYS4 gene of Saccharomyces cerevisiae.
A DNA fragment containing the CYS4 gene of Saccharomyces cerevisiae was isolated from a genomic library. The cloned fragment hybridized to the transverse-alternating-field-electrophoresis band corresponding to chromosomes VII and XV. According to the 2 microns DNA chromosome-loss procedure, the cys2 and cys4 mutations, which are linked together and co-operatively confer cysteine dependence, were assigned to chromosome VII. By further mapping involving tetrad analysis, the cys2-cys4 pair was localized between SUP77 (SUP166) and ade3 on the right arm of chromosome VII.
Ono
B
B
Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Heike
C
C
Yano
Y
Y
Inoue
T
T
Naito
K
K
Nakagami
S
S
Yamane
A
A
D000123:Acetyltransferases / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003541:Cystathionine beta-Synthase / Q000235:genetics; D003545:Cysteine / Q000096:biosynthesis*; D005800:Genes, Fungal; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D051050:Serine O-Acetyltransferase
D000123:Acetyltransferases; D051050:Serine O-Acetyltransferase; D003541:Cystathionine beta-Synthase; D003545:Cysteine