title: Use of gene replacement transformation to elucidate gene function in the qa gene cluster of Neurospora crassa.
abstract: Gene replacement by transformation, employing selective genetic recombination techniques, has been used to delete or disrupt the qa-x, qa-y and qa-1S genes of the qa gene cluster of Neurospora crassa. The growth characteristics of the strain carrying the deletion of the qa-y gene support earlier evidence that this gene encodes a quinic acid permease. The strain containing the deletion of the qa-1S gene (delta qa-1S) was examined with respect to quinic acid induction and carbon catabolite repression. The delta qa-1S strain exhibits constitutive expression of the qa genes supporting earlier evidence that the qa-1S gene codes for a repressor. Several of the qa genes continued to be expressed at high levels even in the presence of glucose in the delta qa-1S strain, which indicates that transcription of these genes is not being affected directly by a repressor molecule in the presence of glucose.
authors.affiliation: Department of Genetics, University of Georgia, Athens 30602.
authors.forename: M E
authors.initials: ME
authors.lastname: Case
authors.forename: R F
authors.initials: RF
authors.lastname: Geever
authors.forename: D K
authors.initials: DK
authors.lastname: Asch
mesh_terms: D002872:Chromosome Deletion; D005800:Genes, Fungal / Q000235:genetics / Q000502:physiology*; D005810:Multigene Family / Q000235:genetics / Q000502:physiology*; D009154:Mutation / Q000502:physiology; D009492:Neurospora crassa / Q000235:genetics*; D011801:Quinic Acid / Q000378:metabolism; D014170:Transformation, Genetic / Q000235:genetics*
chemical_list: D011801:Quinic Acid