Human melanotransferrin (p97) has only one functional iron-binding site.
The iron-binding properties of melanotransferrin, the tumour-associated antigen also known as p97, have been investigated by UV/visible and fluorescence spectroscopy, amino acid sequence comparison, and modelling. These show that, in contrast to other transferrins, melanotransferrin binds only one Fe3+ ion per molecule. The binding properties of its N-terminal site are similar to other transferrins, but its C-terminal site does not bind iron at all. The differences can be related to specific amino acid changes in the C-terminal site.
Baker
E N
EN
Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand.
Baker
H M
HM
Smith
C A
CA
Stebbins
M R
MR
Kahn
M
M
Hellström
K E
KE
Hellström
I
I
D000951:Antigens, Neoplasm; D001665:Binding Sites; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D008545:Melanoma; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D007501:Iron