Sandwich immunoassay of small molecules. II. Effects of heterology and development of a highly sensitive and specific enzyme immunoassay for testosterone.
The effect of dimer heterology in the sandwich immunoassay of testosterone was studied using symmetrical and asymmetrical dimers prepared from testosterone-3-(O-carboxymethyl)oxime and 4-(carboxymethyl-mercapto)testosterone. The effect of antibody heterology was studied using antibodies against 3-carboxymethyl oxime and 17-hemisuccinate derivatives of the steroid and an asymmetrical dimer prepared from the same two derivatives. Using antibody against the 3-carboxymethyl oxime and the dimer of 4-(carboxymethylmercapto)testosterone, the sensitivity of direct enzyme immunoassay of testosterone in serum by this method was 0.5 pg/well and the cross-reactivity of 5 alpha-dihydrotestosterone was 5%.
Sengupta
J
J
Indian Institute of Chemical Biology, Calcutta.
Dhar
T K
TK
Ali
E
E
D000818:Animals; D003429:Cross Reactions; D013196:Dihydrotestosterone; D007124:Immunoenzyme Techniques; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013739:Testosterone / Q000031:analogs & derivatives / Q000032:analysis*
C067733:testosterone-3-carboxymethyloxime; D013196:Dihydrotestosterone; D013739:Testosterone; C029763:testosterone-17-succinate; C073678:4-(carboxymethylmercapto)testosterone