Prostaglandin- or endothelium-mediated vasodilation is not involved in the blunted responses of blood vessels to vasoconstrictors in pregnant rats.
Pregnancy is associated with decreases of blood pressure and vascular sensitivity to vasopressor agents. We have hypothesized that the increased liberation of endogenous vasodilator(s) by the vascular endothelium or other structures could mediate these blunted responses. Thoracic aorta rings of nonpregnant, 21 days pregnant, and first day post partum rats respond similarly to acetylcholine, an endothelium-dependent vasorelaxant. In contrast, the potency of the response to sodium nitroprusside, an endothelium-independent vasorelaxant, is unchanged in tissues of pregnant rats and increased (p less than 0.05) in those of post partum animals. In the presence of indomethacin (10 mumol/L) the three groups of tissues show a decreased potency. The effects of phenylephrine on aortic rings of both nonpregnant and pregnant rats are markedly increased in the presence of Ng-monomethyl-L-arginine. Indeed, the concentration producing 50% of the maximum response of phenylephrine decreases (p less than 0.001) from 50.7 to 8.02, from 93.8 to 37.6, and from 60.4 to 5.97 nmol/L with the use of Ng-monomethyl-L-arginine (0.1 mmol/L) in rings from nonpregnant, pregnant, and postpartum rats, respectively. Simultaneously, the maximum response to phenylephrine increases markedly in the three groups of tissues. In the presence of Ng-monomethyl-L-arginine, indomethacin does not influence the response to phenylephrine. Our results do not support the possible involvement of an endogenous vasodilator (prostaglandin-like or endothelium-derived) in the blunted responses to vasoconstrictors during pregnancy.
St-Louis
J
J
Vascular and Perinatal Pharmacology Laboratory, Hôpital Ste-Justine, Montreal, Quebec, Canada.
Sicotte
B
B
D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000502:physiology; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D009569:Nitric Oxide / Q000502:physiology; D049590:Postpartum Period / Q000502:physiology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011453:Prostaglandins / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D014661:Vasoconstriction / Q000187:drug effects*; D014662:Vasoconstrictor Agents / Q000494:pharmacology*; D014664:Vasodilation
D011453:Prostaglandins; D014662:Vasoconstrictor Agents; D009569:Nitric Oxide