Determination of alpha- and beta-zearalenol and zearalenone in cereals by gas chromatography with ion-trap detection.
A method for determination of estrogenic mycotoxins, alpha- and beta-zearalenol and zearalenone, in cereals (wheat, barley, oats, corn) is described. After extraction with ethylacetate, clean-up involved a base treatment and partition with water; derivatization was by trimethylsilylation. For quantitation and confirmation a capillary gas chromatograph combined with a selective mass detector (ion trap), working in the electron impact-mode was used. The detection limit for the complete method is 1 microgram/kg for each of the three mycotoxins in full scan. Recoveries from spiked cereals were 82-86%.
Schwadorf
K
K
Institute of Animal Nutrition, University of Hohenheim, Stuttgart, Germany.
Müller
H M
HM
D002849:Chromatography, Gas / Q000379:methods; D002523:Edible Grain / Q000737:chemistry*; D013058:Mass Spectrometry; D015025:Zearalenone / Q000032:analysis*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000032:analysis
C029659:zearalenol; D015025:Zearalenone; D015029:Zeranol