Reduced incidence of insulitis in NOD mice following anti-CD3 injection: requirement for neonatal injection.
The incidence of diabetes in NOD mice is reduced following a single neonatal injection of the anti-CD3 antibody, 145.2C11. We now show that the reduction in incidence is greater when the antibody is given in the first than in the third week of life. Anti-CD3 antibody injected in macro-aggregated form did not protect the recipients from insulitis and protection was diminished when elimination of the antibody was accelerated by injecting anti-hamster IgG. Protection was not reversed when anti-CD3 injection was followed by anti-CD4 and anti-CD8. Animals neonatally injected with anti-CD3 were not protected from the induction of diabetes following transfer of spleen cells from diabetic donors. These results contrast with the view that anti-CD3-mediated protection from diabetes depends on a long-lived change in recipient T cells. The findings are consistent with immunosuppression alone being an adequate explanation for the effect of anti-CD3 antibody on susceptibility to diabetes in NOD mice.
Hayward
A R
AR
University of Colorado, School of Medicine, Denver 80262.
Shriber
M
M
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000008:administration & dosage*; D017252:CD3 Complex; D015704:CD4 Antigens / Q000008:administration & dosage; D016827:CD8 Antigens / Q000008:administration & dosage; D003922:Diabetes Mellitus, Type 1 / Q000517:prevention & control*; D005260:Female; D016219:Immunotherapy, Adoptive; D008297:Male; D008562:Membrane Glycoproteins / Q000008:administration & dosage*; D051379:Mice; D016688:Mice, Inbred NOD; D011948:Receptors, Antigen, T-Cell
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D008562:Membrane Glycoproteins; D011948:Receptors, Antigen, T-Cell