title: Features of replication fork blockage by the Escherichia coli terminus-binding protein.
abstract: Blockage of the progress of a DNA replication fork in Escherichia coli can be ascribed to an inhibition of helicase action at the orientation-specific binding of a termination sequence (ter) by the ter-binding protein (Lee, E.H., Kornberg, A., Hidaka, M., Kobayashi, T., and Horiuchi, T. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9104-9108). These observations have been extended to include the PriA helicase, thus confirming that blockage is general for helicases. The site of arrest of synthesis by a replication fork is at the very first nucleotide of the 22-base pair E. coli-terB sequence. Strand displacement by DNA polymerases is also inhibited, but is less profound and is orientation-specific. The ter sequences of plasmids R1-terR and -terL and of plasmids R6K and R100 have been compared with those of E. coli-terA and -terB.
authors.affiliation: Department of Biochemistry, Stanford University School of Medicine, California 94305-5306.
authors.forename: E H
authors.initials: EH
authors.lastname: Lee
authors.forename: A
authors.initials: A
authors.lastname: Kornberg
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis / Q000378:metabolism; D001345:Autoradiography; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D004265:DNA Helicases; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D010957:Plasmids; D013728:Terminator Regions, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C061910:tus protein, Bacteria; C489236:tus protein, E coli; D004259:DNA-Directed DNA Polymerase; D000251:Adenosine Triphosphatases; C506743:priA protein, E coli; D004265:DNA Helicases