title: Point mutation at codon 12 of the c-Ha-ras gene in human gastric cancers.
abstract: The molecular mechanisms of the carcinogenic process of gastric cancer have not been fully understood yet. In order to know whether c-Ha-ras gene is being involved in the process of gastric carcinogenesis, 8 gastric cancer cell lines, 8 cases of gastric cancer and same number of adjacent dysplasia were analyzed for the presence of mutation at codon 12, 13 and 61 of the c-Ha-ras gene by using polymerase chain reaction (PCR) and mutant-specific oligonucleotide hybridization. Point mutations at codon 12 of the c-Ha-ras gene were found in 2 out of 8 gastric cancer and dysplasia samples in one case, but we found no mutation at codon 13 or 61 of the c-Ha-ras gene. These results suggest that the frequency of mutation of the c-Ha-ras gene detected by sensitive PCR technique is low indeed, however it would be notable that such a genetic change has been detected in the dysplastic lesion of the gastric cancer patient.
authors.affiliation: Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: E H
authors.initials: EH
authors.lastname: Koh
authors.forename: H C
authors.initials: HC
authors.lastname: Chung
authors.forename: K B
authors.initials: KB
authors.lastname: Lee
authors.forename: E K
authors.initials: EK
authors.lastname: Han
authors.forename: S H
authors.initials: SH
authors.lastname: Oh
authors.forename: J S
authors.initials: JS
authors.lastname: Min
authors.forename: E M
authors.initials: EM
authors.lastname: Choi
authors.forename: J K
authors.initials: JK
authors.lastname: Youn
authors.forename: B S
authors.initials: BS
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D003062:Codon; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D003062:Codon