The gene encoding the small nuclear ribonucleoprotein-associated protein N is expressed at high levels in neurons.
The highly homologous small nuclear ribonucleoprotein-associated proteins of the Sm group, human N and B/B', are derived from distinct, but similar genes. While the almost identical structural organization of the genes for N and B/B' suggests that they emerged from a common ancestral gene via a duplication event, they now reside on different chromosomes. In contrast to B (which is expressed in all tissues examined) and B' (which is widely expressed with the notable exception of the brain), results from in situ hybridization experiments showed that N is found predominantly in central neurons. Analysis of the transcriptional activity of the 5'-flanking sequences of the human N-encoded gene suggests that the cell-specific expression of N is achieved by selective repression of transcription by distal 5'-flanking sequences.
Schmauss
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Howard Hughes Medical Institute Research Laboratories, Yale University School of Medicine, New Haven, Connecticut 06510.
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Lerner
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D000818:Animals; D001324:Autoantigens / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D005091:Exons; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012261:Ribonucleoproteins / Q000235:genetics*; D017411:Ribonucleoproteins, Small Nuclear; D014158:Transcription, Genetic; D014162:Transfection; D055517:snRNP Core Proteins
D001324:Autoantigens; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins; D004247:DNA