Simultaneous determination of propranolol or metoprolol in the presence of benzodiazepines in the plasma by gas chromatography.
A method for the simultaneous gas chromatographic determination of propranolol and metoprolol and some benzodiazepines in human plasma is described. After the addition of an internal standard, plasma samples were adjusted to pH 8.5 and extracted by SPE chromatography. The analysis was performed by GC with packed and capillary columns, using a FID detector. The quantitative recovery was reported. The assay response was linear in concentration range 50-300 ng/mL for propranolol-benzodiazepine and 100-350 ng/mL for metoprolol-benzodiazepine mixtures.
Quaglio
M P
MP
Dipartimento di Scienze Farmaceutiche, Università di Ferrara.
Bellini
A M
AM
Minozzi
L
L
D001569:Benzodiazepines / Q000097:blood*; D002849:Chromatography, Gas; D006801:Humans; D008790:Metoprolol / Q000097:blood*; D011433:Propranolol / Q000097:blood*
D001569:Benzodiazepines; D011433:Propranolol; D008790:Metoprolol