title: Tract formation and axon fasciculation of molecularly distinct peripheral neuron subpopulations during leech embryogenesis.
abstract: In leech, the central projections of peripheral sensory neurons segregate into specific axonal tracts, which are distinguished by differential expression of surface antigens recognized by the monoclonal antibodies Lan3-2 and Lan4-2. Lan3-2 recognizes an epitope expressed on axons that segregate into three distinct axon fascicles. In contrast, the Lan4-2-positive axons selectively project into only one of the Lan3-2-positive axon tracts. These observations provide evidence for a hierarchy of guidance cues mediating specific pathway formation in this system. Since the Lan3-2 antibody has been shown to perturb this process and since, as shown here, the Lan3-2 and Lan4-2 antigens are closely molecularly interrelated, these antibodies may help define molecules and epitopes mediating neuronal recognition and axonal guidance.
authors.affiliation: Department of Zoology and Genetics, Iowa State University, Ames 50011.
authors.forename: K M
authors.initials: KM
authors.lastname: Johansen
authors.forename: D M
authors.initials: DM
authors.lastname: Kopp
authors.forename: J
authors.initials: J
authors.lastname: Jellies
authors.forename: J
authors.initials: J
authors.lastname: Johansen
mesh_terms: D000344:Afferent Pathways / Q000196:embryology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001369:Axons / Q000648:ultrastructure; D002454:Cell Differentiation; D007150:Immunohistochemistry; D007865:Leeches / Q000196:embryology*; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism*; D010525:Peripheral Nerves / Q000196:embryology*
chemical_list: D000911:Antibodies, Monoclonal; D009419:Nerve Tissue Proteins