title: A new model of equilibrium dysfunction in the rat induced by photochemical damage to the inner ear's microcirculation.
abstract: A new photochemical method was employed to damage the inner ear microcirculation in the rat. Under pentobarbital anesthesia, the middle ear was exposed by a ventral approach and the tympanic membrane and the malleus and incus were removed. The vestibule was then illuminated by a filtered xenon light (wave length: 540 nm) while rose bengal was infused intravenously. Microscopic examination revealed disintegration of the hair cells in the vestibule. Changes could be prevented by pretreatment with intravenous acetylsalicylic acid (ASA) or heparin. Twenty-four hours after the completion of photo-illumination the rats exhibited nystagmus toward the intact ear and showed rolling during the swimming test, both signs of equilibrium dysfunction. These findings were inhibited by ASA or heparin pretreatment. Our present results indicate that our method causes a photochemically induced occlusion in the rat's inner ear microcirculation and may be useful for evaluating the various effects of drugs on the inner ear.
authors.affiliation: Department of Pharmacology, Hamamatsu University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kohno
authors.forename: K
authors.initials: K
authors.lastname: Umemura
authors.forename: Y
authors.initials: Y
authors.lastname: Asai
authors.forename: T
authors.initials: T
authors.lastname: Uematsu
authors.forename: M
authors.initials: M
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007758:Ear, Inner / Q000098:blood supply* / Q000473:pathology; D008027:Light; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000528:radiation effects; D010777:Photochemistry; D004856:Postural Balance / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012395:Rose Bengal / Q000008:administration & dosage
chemical_list: D012395:Rose Bengal