Adapting the uricase/peroxidase procedure for plasma urate to reduce interference due to haemolysis, icterus or lipaemia.
The estimation of serum urate by uricase/peroxidase procedures involving single point bichromatic absorbance monitoring gives inaccurate results with samples which are haemolysed, icteric or lipaemic. These inaccuracies can be greatly minimized by reducing the rate of chromogen formation and applying the assay in a kinetic mode.
Leary
N O
NO
Biochemistry Laboratory, Regional Hospital, Cork, Ireland.
Pembroke
A
A
Duggan
P F
PF
D001663:Bilirubin / Q000097:blood; D005217:Fat Emulsions, Intravenous / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D008055:Lipids / Q000097:blood; D013054:Spectrophotometry, Atomic / Q000295:instrumentation; D014503:Urate Oxidase / Q000097:blood*
D005217:Fat Emulsions, Intravenous; D008055:Lipids; D014503:Urate Oxidase; D001663:Bilirubin