Human cathepsin D precursor is associated with a 60 kDa glycosylated polypeptide.
Human cathepsin D is synthesized as a 53 kDa precursor. Most of it is segregated into lysosomal compartments and subjected to a proteolytic fragmentation. Using a cross-linking reagent we show that a large proportion of the precursor is associated with a distinct protein which--under denaturing and reducing conditions--is characterized as a 60 kDa glycopeptide. Studies on cells cultured in the presence of drugs known to affect the intracellular transport (deoxynojirimycin, brefeldin A and NH4Cl) indicated that the association with cathepsin D precursor occurs early after the synthesis and is at least partially maintained after secretion.
Grässel
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Institut für Physiologische Chemie und Pathobiochemie, Münster, FRG.
Hasilik
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D017485:1-Deoxynojirimycin; D020126:Brefeldin A; D002117:Calcitriol / Q000494:pharmacology; D002402:Cathepsin D / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D002460:Cell Line; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D004792:Enzyme Precursors / Q000235:genetics*; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors; D006031:Glycosylation; D006801:Humans; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational
D003432:Cross-Linking Reagents; D003517:Cyclopentanes; D004792:Enzyme Precursors; D017485:1-Deoxynojirimycin; D020126:Brefeldin A; D006026:Glycoside Hydrolases; C058345:procathepsin D; D002402:Cathepsin D; D002117:Calcitriol; D005944:Glucosamine