Diffusion properties of an artificial membrane used for Langerhans islets encapsulation: an in vitro test.
Glucose and insulin permeability of an artificial membrane (AN69, HOSPAL, Sweden) used for Langerhans islets encapsulation were investigated. In vivo, a 1 and 7 d intraperitoneal implantation of the AN69 membrane in rats induced a loss of permeability towards glucose and insulin probably due to a protein-coating performed after implantation. In vitro, a protein-coating of the AN69 membrane with fetal calf serum solution reproduced similar results. Thus this in vitro test which mimicks in vivo conditions should be proposed to evaluate rapidly the physicochemical properties of a membrane suitable for pancreatic islets encapsulation.
Kessler
L
L
INSERM Unité 61, Biologie Cellulaire et Physiopathologie Digestives, Strasbourg, France.
Aprahamian
M
M
Keipes
M
M
Damgé
C
C
Pinget
M
M
Poinsot
D
D
D000180:Acrylic Resins; D000181:Acrylonitrile / Q000031:analogs & derivatives; D000818:Animals; D001672:Biocompatible Materials; D004058:Diffusion; D005947:Glucose / Q000493:pharmacokinetics; D007328:Insulin / Q000493:pharmacokinetics; D007515:Islets of Langerhans / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D008422:Materials Testing; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010539:Permeability; D051381:Rats
D000180:Acrylic Resins; D001672:Biocompatible Materials; D007328:Insulin; D008567:Membranes, Artificial; C016892:AN-69; D005947:Glucose; D000181:Acrylonitrile