title: Construction of a broad-host-range non-mobilizable stable vector carrying RP4 par-region.
abstract: Plasmid pXL1635 was constructed from the already segregationally stable incP-derived pRK290. Plasmid pXL1635 should be suitable for industrial and environmental uses in Gram- bacteria since (i) it contains the par fragment from RP4 which increases its stability in Pseudomonas denitrificans, a cobalamin-producing and industrially used bacterium, and (ii) the RK2 oriT has been deleted, leading to a non-mobilizable plasmid.
authors.affiliation: Département Biotechnologies, Rhône-Poulenc Rorer S.A., Vitry sur Seine, France.
authors.forename: J
authors.initials: J
authors.lastname: Crouzet
authors.forename: S
authors.initials: S
authors.lastname: Lévy-Schil
authors.forename: L
authors.initials: L
authors.lastname: Cauchois
authors.forename: B
authors.initials: B
authors.lastname: Cameron
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D013045:Species Specificity