title: The immunity-conferring plasmid p phi HL from the Halobacterium salinarium phage phi H: nucleotide sequence and transcription.
abstract: The complete nucleotide sequence of the plasmid p phi HL, composing the central 12,041-bp L-region from the temperate phage phi H of Halobacterium salinarium is presented. Transcripts mapped to the p phi HL and the L-region produced under immune conditions, under lytic growth or constitutively, are described. The sequences upstream of the transcription start points show homology to the consensus sequence for archaeal (formerly archaebacterial) promoters. Lytic transcription is shown to be strictly time-dependent, with an early gene product required for the expression of late genes.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Gropp
authors.forename: B
authors.initials: B
authors.lastname: Grampp
authors.forename: P
authors.initials: P
authors.lastname: Stolt
authors.forename: P
authors.initials: P
authors.lastname: Palm
authors.forename: W
authors.initials: W
authors.lastname: Zillig
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D006217:Halobacterium / Q000235:genetics*; D007700:Kinetics; D008242:Lysogeny; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral