title: Three C-phycoerythrin-associated linker polypeptides in the phycobilisome of green-light-grown Calothrix sp. PCC 7601 (cyanobacteria).
abstract: Microanalyses by SDS-PAGE and microsequencing demonstrate that, under green-light conditions, 3 C-phycoerythrin associated rod-linker polypeptides with different N-terminal amino acid sequences are present in phycobilisomes (PBS) from Calothrix sp. 7601 cells. Two of these polypeptides, corresponding to SDS-PAGE bands at 36 and 37 kDa, could be assigned, respectively, to the cpeC and cpcD genes found on a separate cpeCD-operon in Calothrix sp. 7601 (Federspiel, N.A. and Grossman, A.R. (1990) J. Bacteriol, 172, 4072-4081). The third C-PE rod-linker polypeptide, LR,2PE,33, requires, therefore, a third gene with the suggested locus designation 'cpeE'. A C-PE (alpha beta)6-LR,2PE,33 complex containing this third rod-linker polypeptide could be isolated from phycobilisomes and characterized. PBS from both green- and red-light cells of Calothrix contain a single, unique LRC28 rod-core linker polypeptide which is not altered during chromatic adaptation.
authors.affiliation: Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule, Zürich, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Glauser
authors.forename: W A
authors.initials: WA
authors.lastname: Sidler
authors.forename: K W
authors.initials: KW
authors.lastname: Graham
authors.forename: D A
authors.initials: DA
authors.lastname: Bryant
authors.forename: G
authors.initials: G
authors.lastname: Frank
authors.forename: E
authors.initials: E
authors.lastname: Wehrli
authors.forename: H
authors.initials: H
authors.lastname: Zuber
mesh_terms: D000595:Amino Acid Sequence; D000458:Cyanobacteria / Q000378:metabolism* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009876:Operon; D010455:Peptides / Q000378:metabolism*; D045524:Phycobilisomes; D010799:Phycoerythrin / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D010455:Peptides; D045524:Phycobilisomes; D010799:Phycoerythrin