title: Stimulation of DNA synthesis by rat plasma following in vivo treatment with three liver mitogens.
abstract: The present study was undertaken to determine the effect of two different types of liver cell proliferative stimuli, namely compensatory regeneration and direct hyperplasia on DNA synthesis of normal and preneoplastic isolated hepatocytes. Platelet-poor plasma (PPP) isolated from male Wistar rats treated with three different hepato-mitogens, lead nitrate (LN), cyproterone acetate (CPA) and ethylene dibromide (EDB), or subjected to surgical partial hepatectomy (PH), was tested for its ability to stimulate DNA synthesis in normal and preneoplastic hepatocytes in primary cultures. Induction of DNA synthesis was detected as early as 30 min after CPA, EDB and PH administration and persisted up to 5 days after the LN administration. In addition, hepatocytes isolated from preneoplastic liver nodules were also able to respond in culture to the DNA synthesis stimulus induced by these factors.
authors.affiliation: Istituto di Patologia Sperimentale, Università di Cagliari, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Coni
authors.forename: G
authors.initials: G
authors.lastname: Pichiri-Coni
authors.forename: G M
authors.initials: GM
authors.lastname: Ledda-Columbano
authors.forename: E
authors.initials: E
authors.lastname: Semple
authors.forename: S
authors.initials: S
authors.lastname: Rajalakshmi
authors.forename: P M
authors.initials: PM
authors.lastname: Rao
authors.forename: D S
authors.initials: DS
authors.lastname: Sarma
authors.forename: A
authors.initials: A
authors.lastname: Columbano
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003534:Cyproterone / Q000031:analogs & derivatives / Q000494:pharmacology; D017373:Cyproterone Acetate; D004247:DNA / Q000096:biosynthesis*; D015946:Ethylene Dibromide / Q000494:pharmacology; D006133:Growth Substances / Q000096:biosynthesis / Q000097:blood* / Q000494:pharmacology; D007854:Lead / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology; D008297:Male; D008934:Mitogens / Q000494:pharmacology*; D009566:Nitrates / Q000494:pharmacology; D011230:Precancerous Conditions / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
chemical_list: D006133:Growth Substances; D008934:Mitogens; D009566:Nitrates; D015946:Ethylene Dibromide; D007854:Lead; D017373:Cyproterone Acetate; C017461:lead nitrate; D004247:DNA; D003534:Cyproterone