title: An extra copy of nimEcyclinB elevates pre-MPF levels and partially suppresses mutation of nimTcdc25 in Aspergillus nidulans.
abstract: Previous work has shown that nimA encodes a cell cycle regulated protein kinase that is required along with the p34cdc2 histone H1 kinase (MPF) for mitosis in Aspergillus nidulans. We have now identified two other gene products required for mitosis in A.nidulans. nimT encodes a protein similar to the fission yeast cdc25 tyrosine phosphatase and is required for the conversion of pre-MPF to MPF and nimE encodes a B-type cyclin which is a subunit of MPF. A new genetic interaction between nimEcyclinB and nimTcdc25 type genes is reported. Increased copy number of nimEcyclinB can suppress mutation of nimTcdc25 and also lead to increased accumulation of tyrosine phosphorylated p34cdc2 (pre-MPF). This biochemical observation suggests an explanation for the genetic complementation. If nimEcyclinB recruits p34cdc2 for tyrosine phosphorylation to form pre-MPF it follows that increased expression of nimEcyclinB would increase the level of pre-MPF. The increased level of pre-MPF generated may then allow the mutant nimTcdc25 protein to convert enough pre-MPF to MPF and thus permit some mitotic progression. We also demonstrate that correct cell cycle regulation by the p34cdc2 protein kinase pathway is essential for correct developmental progression in A.nidulans.
authors.affiliation: Department of Pharmacology, UMDNJ-Robert Wood Johnson Medical School, Piscataway 08854.
authors.forename: M J
authors.initials: MJ
authors.lastname: O'Connell
authors.forename: A H
authors.initials: AH
authors.lastname: Osmani
authors.forename: N R
authors.initials: NR
authors.lastname: Morris
authors.forename: S A
authors.initials: SA
authors.lastname: Osmani
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D016203:CDC2 Protein Kinase / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics*; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D003360:Cosmids; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D005800:Genes, Fungal; D016200:Maturation-Promoting Factor / Q000235:genetics*; D008938:Mitosis / Q000235:genetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013489:Suppression, Genetic
chemical_list: D016213:Cyclins; D005656:Fungal Proteins; D009838:Oligodeoxyribonucleotides; D011498:Protein Precursors; C067034:maturation-promoting factor precursor; C075058:NIME protein, Emericella nidulans; D011494:Protein Kinases; D016203:CDC2 Protein Kinase; D016200:Maturation-Promoting Factor