title: Functional reconstitution of the isolated erythrocyte water channel CHIP28.
abstract: Measurements of water permeability indicate the existence of a facilitated water transporting pathway in erythrocytes, kidney tubules and amphibian urinary bladder. Two lines of evidence suggest that one type of water channel is an approximately 30-kDa protein: the approximately 30-kDa target size determined by radiation inactivation (van Hoek, A. N., Hom, M. L., Luthjens, L. H., de Jong, M. D., Dempster, J. A., and van Os, C. H. (1991) J. Biol. Chem. 266, 16633-16635) and the increased water permeability in oocytes that express mRNA encoding a 28-kDa erythrocyte protein (CHIP28, Preston, B. M., Carroll, T. P., Guggino, W. B., and Agre, P. (1992) Science 256, 385-387). We report direct evidence that CHIP28 is the erythrocyte water channel. Osmotic water permeability (Pf) remained high (0.029 cm/s, 37 degrees C) when erythrocyte membranes were stripped of nearly all proteins except for CHIP28. N-terminal sequence analysis confirmed that the 28-kDa protein was CHIP28. Pf in proteoliposomes reconstituted with solubilized CHIP28 was high (Pf = 0.03 cm/s, 37 degrees C), the activation energy was low (2.2 kcal/mol), and Pf was decreased by greater than 50-fold by mercurial sulfhydryl reagents and Me2SO. The single-channel water permeability was approximately 10(-13) cm3/s, slightly higher than that of the gramicidin A channel. The water channel excluded the small solute urea. These data establish a procedure to reconstitute functional water channels into liposomes and demonstrate that CHIP28 is the erythrocyte water channel.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143-0532.
authors.forename: A N
authors.initials: AN
authors.lastname: van Hoek
authors.forename: A S
authors.initials: AS
authors.lastname: Verkman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000235:genetics; D002463:Cell Membrane Permeability; D004231:Diuresis; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000502:physiology; D008969:Molecular Sequence Data; D011510:Proteolipids; D012333:RNA, Messenger / Q000378:metabolism; D014867:Water / Q000378:metabolism*; D014981:Xenopus
chemical_list: D001798:Blood Proteins; D011510:Proteolipids; D012333:RNA, Messenger; C020485:proteoliposomes; D014867:Water