Successful 96-Hr cold-storage preservation of canine pancreas with UW solution containing the thromboxane A2 synthesis inhibitor OKY046.
Prostanoids, such as prostacyclin (PGI) and thromboxane A2 (TxA), have been recently suggested to play an important role in preservation-induced injury of pancreas grafts. We have previously shown that the TxA synthesis inhibitor OKY046 prevents a decrease of both the PGI/TxA ratio and blood flow in pancreas grafts after 24-hr preservation with Euro-Collins solution. In our present study, we analyzed whether OKY046 added to University of Wisconsin (UW) solution could extend successful cold-storage preservation of segmental canine pancreas grafts, compared with UW alone. We divided 30 dogs into four preservation groups: Group 1, UW solution for 72 hr (n = 7); Group 2, UW solution for 96 hr (n = 8); Group 3, UW solution plus OKY046 (10(-4) M) for 72 hr (n = 7); and Group 4, UW solution plus OKY046 (10(-4) M) for 96 hr (n = 8). After the cold storage period, segmental pancreas auto-transplantation with immediate completion pancreatectomy was done. Preservation was deemed successful if serum glucose less than 150 mg/dl was maintained for at least 5 days. Intravenous glucose tolerance tests and biopsies were done in those dogs with functioning grafts 14 days post-transplant. Successful preservation rates were as follows: Group 1, 57.1%; Group 2, 12.5%; Group 3, 100%; and Group 4, 75%. The mean K values (+/- standard error) were: Group 1, 1.54 +/- 0.13; Group 2, 0.59; Group 3, 1.54 +/- 0.14; and Group 4, 1.59 +/- 0.24 (not statistically different).(ABSTRACT TRUNCATED AT 250 WORDS)
Kin
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Department of Surgery, University of Minnesota, Minneapolis 55455.
Stephanian
E
E
Gores
P
P
Shirabe
K
K
Tanaka
T
T
Salazar
A
A
Brayman
K
K
Gruessner
R
R
Sutherland
D E
DE
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003080:Cold Temperature; D004285:Dogs; D011464:Epoprostenol / Q000502:physiology; D005260:Female; D005978:Glutathione; D007328:Insulin; D008297:Male; D008689:Methacrylates / Q000494:pharmacology*; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D016035:Pancreas Transplantation; D011887:Raffinose; D012996:Solutions; D013930:Thromboxane-A Synthase / Q000037:antagonists & inhibitors*; D013931:Thromboxanes / Q000502:physiology; D013997:Time Factors
D007328:Insulin; D008689:Methacrylates; D019188:Organ Preservation Solutions; D012996:Solutions; D013931:Thromboxanes; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D011464:Epoprostenol; D013930:Thromboxane-A Synthase; D005978:Glutathione; D000241:Adenosine; C034364:ozagrel; D011887:Raffinose