Induction of 6-thioguanine-resistant mutants in human diploid fibroblasts in vitro with ethylene oxide.
Human diploid fibroblasts (strain VH-10) were exposed to the direct-acting alkylating agent, ethylene oxide (EtO), in vitro, and the frequency of HPRT mutants was evaluated by selection in medium containing 6-thioguanine. A dose-dependent increase of the mutant frequency was found in the dose range of 2.5-10 mMh of EtO. The EtO-induced mutant frequency increased 5-19 times the background frequency at low or moderately toxic doses, which indicates that EtO is a strong mutagen in human fibroblasts in vitro. The mutagenic potency was 9.8 x 10(-6) per mMh.
Kolman
A
A
Department of Radiobiology, Stockholm University, Sweden.
Bohusová
T
T
Lambert
B
B
Simons
J W
JW
D000477:Alkylating Agents / Q000633:toxicity; D002470:Cell Survival; D002478:Cells, Cultured; D004171:Diploidy; D004351:Drug Resistance; D005027:Ethylene Oxide / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D008297:Male; D009153:Mutagens; D013866:Thioguanine
D000477:Alkylating Agents; D009153:Mutagens; D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine; D005027:Ethylene Oxide