Biotin transport in primary culture of astrocytes: effect of biotin deficiency.
The uptake of radioactive biotin has been studied in glial cell cultures from dissociated cerebral hemispheres of newborn rats. We describe saturable kinetics for the biotin uptake at biotin concentrations of less than 60 nM and linear at greater than 60 nM. The uptake appeared temperature sensitive, Na+ independent, nonsensitive to valinomycin, and not affected by metabolic inhibitors such as sodium fluoride or azide. Lipoic acid and biocytin were effective in inhibiting the biotin uptake. These findings are consistent with biotin uptake by the primary culture of astrocytes as a process of facilitated diffusion. Moreover, biotin uptake in astrocytes grown in biotin-restricted conditions was significantly higher compared with the control. This increase appeared mediated through a pronounced increase (10-fold) in the Vmax of the biotin uptake without any change in the apparent Km.
Rodriguez-Pombo
P
P
Departamento de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Ugarte
M
M
D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000502:physiology; D001692:Biological Transport; D001710:Biotin / Q000172:deficiency / Q000493:pharmacokinetics*; D002478:Cells, Cultured; D008239:Lysine / Q000031:analogs & derivatives / Q000494:pharmacology; D008564:Membrane Potentials; D008063:Thioctic Acid / Q000494:pharmacology; D014634:Valinomycin / Q000494:pharmacology
D014634:Valinomycin; D001710:Biotin; D008063:Thioctic Acid; C013411:biocytin; D008239:Lysine