title: Ultrastructural localization of glycoconjugates in human bronchial glands: the subcellular organization of N- and O-linked oligosaccharide chains.
abstract: We investigated the glycoconjugates of the human bronchial glands at light and electron microscopic level by means of lectin histochemistry in combination with neuraminidase digestion and beta-elimination reaction. Both direct and indirect techniques using lectin-peroxidase, lectin-gold, and glycoprotein-gold complexes were applied. The binding pattern of the six lectins (ConA, HPA, DSA, WGA, LEA, and PNA) used in the present study suggests that mucous and serous cells of human bronchial glands contain both N- and O-glycosylated proteins in the secretory granules. Asparagine-linked oligosaccharides containing Gal(beta-1,4) GlcNAc and Man residues were abundant in serous cells. The demonstration of both the terminal Neu 5Ac (alpha-2,3, or 6) Gal (beta-1,4) GlcNAc sequence in the N-linked oligosaccharides of mucous cells and the terminal disaccharide Gal (beta-1,4) GlcNAc in the N-linked oligosaccharide chains of serous cells suggests the existence of complex type sugar chains N-glycosidically linked to the peptide region of the glycoproteins. The binding pattern of the DSA and the neuraminidase-DSA sequence provides evidence for the existence of sialyltransferase activity in the forming mucous granules of mucous bronchial cells.
authors.affiliation: Department of Cell Biology, Medical School, University of Murcia, Spain.
authors.forename: M T
authors.initials: MT
authors.lastname: Castells
authors.forename: J
authors.initials: J
authors.lastname: Ballesta
authors.forename: J F
authors.initials: JF
authors.lastname: Madrid
authors.forename: J A
authors.initials: JA
authors.lastname: Martínez-Menárguez
authors.forename: M
authors.initials: M
authors.lastname: Avilés
mesh_terms: D001216:Asparagine; D001980:Bronchi / Q000473:pathology / Q000648:ultrastructure*; D002240:Carbohydrate Sequence; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004187:Disaccharides / Q000032:analysis; D006001:Glycoconjugates / Q000032:analysis* / Q000737:chemistry; D006056:Golgi Apparatus / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins; D008175:Lung Neoplasms / Q000473:pathology / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009092:Mucous Membrane / Q000473:pathology / Q000648:ultrastructure; D009844:Oligosaccharides / Q000032:analysis*
chemical_list: D004187:Disaccharides; D006001:Glycoconjugates; D037102:Lectins; D009844:Oligosaccharides; D001216:Asparagine