Amino acid and cDNA sequences of a neutral phospholipase A2 from the long-nosed viper (Vipera ammodytes ammodytes) venom.
The amino acid sequence of a non-toxic phospholipase A2, ammodytin I2, from the venom of the long-nosed viper (Vipera ammodytes ammodytes) and its cDNA sequence have been determined. The protein sequence was elucidated by sequencing the peptides generated by CNBr cleavage, mild acid hydrolysis and tryptic digestion of maleylated and non-maleylated protein. Sequencing of the cDNA showed that the protein is synthesized as an 137-amino-acid-residue precursor molecule consisting of a 16-residue signal peptide, followed by a 121-residue mature enzyme. Ammodytin I2 cDNA shows 73% nucleotide and 59% amino acid identities in the mature protein region in comparison to that of ammodytoxin A, the most presynaptically neurotoxic phospholipase A2 from the long-nosed viper. Identities in the signal-peptide region are considerably higher, 96% and 100%, respectively.
Krizaj
I
I
Department of Biochemistry, Jozef Stefan Institute, Ljubljana, Slovenia.
Liang
N S
NS
Pungercar
J
J
Strukelj
B
B
Ritonja
A
A
Gubensek
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D010449:Peptide Mapping; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D021382:Protein Sorting Signals; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014757:Viper Venoms / Q000201:enzymology* / Q000235:genetics*
D010446:Peptide Fragments; D021382:Protein Sorting Signals; D014757:Viper Venoms; C073683:ammodytin I(2); D004247:DNA; C068117:ammodytoxin A; D010741:Phospholipases A; D054467:Phospholipases A2