title: [Isolation and properties of intracellular peptidase from Brevibacterium].
abstract: The intracellular peptidase of Brevibacterium E531, a lysine-producing bacterial species, was purified 6500-fold by chromatography on DEAE-cellulose and the affinity adsorbent H-Thr(But)-Phe-Pro-hexamethylene-diamine-Sepharose 4B and by gel filtration on Sephadex G-200. The enzyme displayed the maximum activity towards proline p-nitroanilide at pH 7.7-7.9 and readily split glycine, alanine and proline from di-, tri- and tetrapeptides but did not practically hydrolyze oligopeptides of a greater chain length. The enzyme was not inhibited by complexons (EDTA, 8-oxiquinoline and 1.10-phenanthroline). The peptidase was not activated by divalent metal ions and was inhibited by Zn2+; Cd2+, Hg2+ and Cu2+. Data brom gel filtration on Sephadex G-200 suggest that the molecular mass of the enzyme is no less than 250 kDa. In the presence of sodium dodecyl sulfate the molecular mass of the enzyme is 43 kDa, which is suggestive of the presence of a quaternary structure. One peculiarity of the enzyme is its activation by alkaline metal halogenides and sodium nitrate which reaches a maximum at the 0.05-0.1 M concentration of the salts.
authors.forename: S E
authors.initials: SE
authors.lastname: Movsesian
authors.forename: T I
authors.initials: TI
authors.lastname: Vaganova
authors.forename: Z M
authors.initials: ZM
authors.lastname: Zaĭstseva
authors.forename: R G
authors.initials: RG
authors.lastname: Ovumian
authors.forename: E A
authors.initials: EA
authors.lastname: Timokhina
authors.forename: V M
authors.initials: VM
authors.lastname: Stepanov
mesh_terms: D000626:Aminopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D001951:Brevibacterium / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007477:Ions; D008670:Metals / Q000494:pharmacology
chemical_list: D007477:Ions; D008670:Metals; D000626:Aminopeptidases
vernacular_title: Vydelenie i svoĭstva vnutrikletochnoĭ peptidazy Brevibacterium.