Point mutation at codon 12 of the c-Ha-ras gene in human gastric cancers.
The molecular mechanisms of the carcinogenic process of gastric cancer have not been fully understood yet. In order to know whether c-Ha-ras gene is being involved in the process of gastric carcinogenesis, 8 gastric cancer cell lines, 8 cases of gastric cancer and same number of adjacent dysplasia were analyzed for the presence of mutation at codon 12, 13 and 61 of the c-Ha-ras gene by using polymerase chain reaction (PCR) and mutant-specific oligonucleotide hybridization. Point mutations at codon 12 of the c-Ha-ras gene were found in 2 out of 8 gastric cancer and dysplasia samples in one case, but we found no mutation at codon 13 or 61 of the c-Ha-ras gene. These results suggest that the frequency of mutation of the c-Ha-ras gene detected by sensitive PCR technique is low indeed, however it would be notable that such a genetic change has been detected in the dysplastic lesion of the gastric cancer patient.
Koh
E H
EH
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
Chung
H C
HC
Lee
K B
KB
Han
E K
EK
Oh
S H
SH
Min
J S
JS
Choi
E M
EM
Youn
J K
JK
Kim
B S
BS
D001483:Base Sequence; D003062:Codon; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*
D003062:Codon