Effect of topical retinoic acids on the levels of collagen mRNA during the repair of UVB-induced dermal damage in the hairless mouse and the possible role of TGF-beta as a mediator.
Topically applied retinoic acids have been found to enhance the gene expression for collagen types I and III in the skin of UVB-irradiated hairless mice. Prior damage is required because the effect is not observed in the skin of age-matched, non-irradiated control animals. Immunochemical methods have shown an increase in TGF-beta 1 and, to a lesser extent, of TGF-beta 2 in the epidermis following retinoic acid treatment. There were no changes in mRNA levels for any of the isotypes of TGF-beta induced by retinoic acid treatment. This study suggests that TGF-beta may mediate the effect of retinoic acids on dermal repair through the stimulation of collagen gene expression.
Kim
H J
HJ
Preclinical Research, Roche Dermatologics, Hoffmann-La Roche Inc., Nutley, New Jersey 07110-1199.
Bogdan
N J
NJ
D'Agostaro
L J
LJ
Gold
L I
LI
Bryce
G F
GF
D000287:Administration, Topical; D000818:Animals; D003094:Collagen / Q000235:genetics*; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D012333:RNA, Messenger / Q000032:analysis*; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D016212:Transforming Growth Factor beta / Q000502:physiology*; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014212:Tretinoin; D003094:Collagen