Flow cytometric analysis of colorectal mucosa from patients with Crohn's disease, ulcerative colitis and cancer.
Samples of colorectal mucosa from patients with Crohn's disease, ulcerative colitis and cancer were analyzed by means of flow cytometry. S- and G2-phase fractions were determined and mean values were calculated for different groups of patients. Almost identical results were obtained for inflamed and normal appearing mucosa from patients with Crohn's disease as well as inflamed mucosa from patients with ulcerative colitis. The mean S- and G2-phase fractions in normal appearing mucosa from cancer patients, however, were significantly higher. This seems to be due to the fact that patients with Crohn's disease are between 15 and 45 years old, while cancer patients are mostly over 45. A detailed analysis of the S- and G2-phase fractions in different age groups revealed a slight, but significant increase in colorectal proliferation between 25 and 75 years.
Zölzer
F
F
Institut für Medizinische Strahlenbiologie, Universitätsklinikum Essen, BRD.
Streffer
C
C
van Beuningen
D
D
Pelzer
T
T
Gross
E
E
Eigler
F W
FW
D000328:Adult; D000367:Age Factors; D002453:Cell Cycle; D003093:Colitis, Ulcerative / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000473:pathology*; D003424:Crohn Disease / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D007414:Intestinal Neoplasms / Q000473:pathology; D008875:Middle Aged