title: Ultrastructural localization of L-fucose residues in nuclei of root primordia of the green pea Pisum sativum.
abstract: Sugars have been demonstrated in animal cell nuclei, but only a few studies have mentioned their presence in plant cell nuclei. In this study L-fucose residues were localized at the ultrastructural level, using Ulex europeaus agglutinin I lectin, during the early stages of germination of Pisum sativum and in mature root tip cells. This sugar was present after 1 h of germination, and its concentration was found to vary during 3 to 6 h inhibition; after 72h of inhibition its concentration had more than doubled. Furthermore, labelling was particularly abundant in the nucleolus, nucleolus-associated bodies and dense nuclear bodies. The possibility that some of the L-fucose residues are associated with proteins is discussed.
authors.affiliation: Biology Department, Faculty of Science and Engineering, Laval University, Quebec, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Chamberland
authors.forename: J G
authors.initials: JG
authors.lastname: Lafontaine
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D007887:Fabaceae / Q000166:cytology*; D005643:Fucose / Q000032:analysis* / Q000378:metabolism; D006046:Gold; D006651:Histocytochemistry / Q000379:methods; D037102:Lectins; D008854:Microscopy, Electron / Q000379:methods; D037121:Plant Lectins; D010946:Plants, Medicinal
chemical_list: D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D005643:Fucose; D006046:Gold