Interactions between proteins bound to the duck beta A-globin gene promoter and enhancer detected by the DNaseI footprinting.
The duck beta A-globin (beta AGLB) enhancer was closely linked to the duck beta A-GLB promoter, and the construct was used to study binding of nuclear proteins to specific sites of these regulatory elements. DNaseI-footprint analysis showed that the presence of the enhancer induced binding of proteins to additional sites on the promoter. The results are consistent with the looping-out model, based on specific interactions of enhancer-bound and promoter bound-proteins.
Cvekl
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Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague.
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D000818:Animals; D004247:DNA / Q000378:metabolism; D015342:DNA Probes / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D004372:Ducks / Q000235:genetics*; D004586:Electrophoresis; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D005914:Globins / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D015342:DNA Probes; D014157:Transcription Factors; D005914:Globins; D004247:DNA; D003850:Deoxyribonuclease I