Lack of a role of interleukin 11 in the growth of multiple myeloma.
Interleukin (IL) 11 is a recently described lymphokine which, like IL-6, stimulates normal hematopoietic murine and human hematopoietic progenitor cells and therefore has potential value for either enhancing hematopoiesis in disease states or augmenting hematopoietic recovery after myeloablative therapies. Since IL-6 is known to promote the growth of human myeloma, either in an autocrine or paracrine fashion, we examined the effect of IL-11 on the growth of a murine plasmacytoma cell line, human myeloma-derived cell lines, and freshly isolated human myeloma cells. Interleukin 11 does increase DNA synthesis by the murine plasmacytoma line T10 in the presence of neutralizing antibody to IL-6. However, neither human myeloma cells nor derived cell lines express IL-11 mRNA; secrete IL-11; express IL-11 cell surface receptors; or augment either DNA synthesis or Ig secretion in response to exogenous IL-11. These findings strongly suggest that IL-11 does support the growth of a murine plasmacytoma cell line but does not play a role in the growth of either freshly isolated human myeloma cells or derived cell lines.
Paul
S R
SR
Division of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA 02115.
Barut
B A
BA
Bennett
F
F
Cochran
M A
MA
Anderson
K C
KC
D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division / Q000502:physiology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D007136:Immunoglobulins / Q000502:physiology; D017370:Interleukin-11; D015850:Interleukin-6 / Q000502:physiology; D007378:Interleukins / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D007700:Kinetics; D051379:Mice; D009101:Multiple Myeloma / Q000378:metabolism / Q000473:pathology*; D010954:Plasmacytoma / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D007136:Immunoglobulins; D017370:Interleukin-11; D015850:Interleukin-6; D007378:Interleukins; D012333:RNA, Messenger