title: In vivo cytotoxic T lymphocyte induction with soluble proteins administered in liposomes.
abstract: The in vivo induction of a CTL response usually requires that Ag be endogenously synthesized so that appropriate processing can occur. In most of the few examples where successful CTL induction was reported with proteins and peptides, unacceptable adjuvants or means of Ag formulation were used. In the present report, liposomes were used to incorporate the soluble proteins OVA and beta-galactosidase. This simple and convenient to use approach, which requires minimal amounts of Ag, results in priming for a CD8+ CTL response and the establishment of immunologic memory. The liposome approach may not only prove a convenient means of inducing CTL responses in vivo but may also be useful to study the mechanisms of Ag processing.
authors.affiliation: Department of Microbiology, College of Veterinary Medicine, University of Tennessee, Knoxville 37996-0845.
authors.forename: R
authors.initials: R
authors.lastname: Reddy
authors.forename: F
authors.initials: F
authors.lastname: Zhou
authors.forename: S
authors.initials: S
authors.lastname: Nair
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.forename: B T
authors.initials: BT
authors.lastname: Rouse
mesh_terms: D000818:Animals; D016827:CD8 Antigens / Q000032:analysis; D005260:Female; D008081:Liposomes / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*; D001616:beta-Galactosidase / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D016827:CD8 Antigens; D008081:Liposomes; D010047:Ovalbumin; D001616:beta-Galactosidase