2'-Amino-2'-deoxyguanosine is a cofactor for self-splicing in group I catalytic RNA.
In investigations on self-splicing in the group I intron of the pre-mRNA from the nrdB gene of bacteriophage T4 it was found that 2'-amino-2'-deoxyguanosine can replace guanosine as cofactor. This is the first guanosine-analogue with a modification in the 2'-position and substantial activity in a group I self-splicing reaction. The results suggest that the 2'-amino and 2'-hydroxy groups of the cosubstrates have some properties in common, which are important for binding as well as for catalysis.
Strömberg
R
R
Department of Organic Chemistry, Stockholm University, Sweden.
Hahne
S
S
Sjögren
A S
AS
Sjöberg
B M
BM
D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D006151:Guanosine / Q000031:analogs & derivatives / Q000378:metabolism*; D007438:Introns; D012322:RNA Precursors / Q000378:metabolism*; D016337:RNA, Catalytic / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013604:T-Phages / Q000378:metabolism*
D012322:RNA Precursors; D016337:RNA, Catalytic; D006151:Guanosine; C014319:2'-amino-2'-deoxyguanosine; D003849:Deoxyguanosine