Gomisin A inhibits tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin.
Gomisin A, isolated from the fruits of Schisandra chinensis, is one of the dibenzocyclooctadiene lignans. Application of 12-O-tetradecanoylphorbol-13-acetate (TPA, 1 microgram/ear), a tumor-promoting agent, to the ears of mice induces inflammation. Among seven dibenzocyclooctadiene lignans assayed, gomisin A, gomisin J, and wuweizisu C inhibited the inflammatory activity induced by TPA in mice. The ED50 of these compounds for TPA-induced inflammation was 1.4-4.4 mumol. Gomisin A, with an ED50 of 1.4 mumol, showed the strongest inhibitory effect. Furthermore, at 5 mumol/mouse, it markedly suppressed the promotion effect of TPA (2.5 micrograms/mouse) on skin tumor formation in mice following initiation with 7,12-dimethylbenz[a]anthracene (50 micrograms/mouse). It is assumed that the inhibition of tumor promotion by gomisin A is due to its anti-inflammatory activity.
Yasukawa
K
K
College of Pharmacy, Nihon University, Chiba, Japan.
Ikeya
Y
Y
Mitsuhashi
H
H
Iwasaki
M
M
Aburada
M
M
Nakagawa
S
S
Takeuchi
M
M
Takido
M
M
D000818:Animals; D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000494:pharmacology*; D034242:Cyclooctanes; D004149:Dioxoles; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D017705:Lignans; D051379:Mice; D008813:Mice, Inbred ICR; D011083:Polycyclic Compounds / Q000737:chemistry / Q000494:pharmacology*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives
D016588:Anticarcinogenic Agents; D034242:Cyclooctanes; D004149:Dioxoles; D004365:Drugs, Chinese Herbal; D017705:Lignans; D011083:Polycyclic Compounds; C033585:schizandrol B; C032975:12-O-tetradeca-2,4,6,8-tetranoylphorbol-13-acetate; C031409:schizandrin C; D013755:Tetradecanoylphorbol Acetate