[Inhibitory effect of vitamin E on rats with bladder tumors induced by N-butyl-N-(4-hydroxybutyl) nitrosamine].
The anticancer activity of vitamin E may involve its functions as an antioxidant free radical scavenger, and immunomodulater. We studied the effect of vitamin E on rats with bladder tumors induced by N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN). Three-week-old male Wistar rats were given a diet containing 0 (A), 2 mg (B:control), 40 mg (C), and 60 mg (D) of vitamin E per 100 g of food, respectively, until they were 30 weeks old. The carcinogen, 0.05% BBN was given in drinking water to the rats for 8 weeks from the 9th week of age, and all rats were killed at 30 weeks old. The number and the size of the tumors, the thickness of epithelial cell layers in hyperplasia, the diameter of papillary tumor, and the cellular atypia were assessed by using a point method according to U-test. As assessment of immune response, the lymphocyte count in blood, the degree of lymphocyte infiltration in tissue, and CD4 (helper/inducer-T cell)/CD8 (suppressor/cytotoxic-T cell) ratio in blood by flow cytometry using monoclonal antibodies were evaluated according to the t-test. Moreover, the correlation between CD4/CD8, cellular atypia, and serum level of vitamin E were evaluated by coefficient of correlation. There were no differences between any two groups in the number or the size of the tumors. The cellularity in group A was significantly higher than that in group D (p less than 0.05). The point of the cellular atypia tended to decrease, with increasing dose of vitamin E, but not significantly, whereas the CD4/CD8 tended to increase by increasing the dose of vitamin E.(ABSTRACT TRUNCATED AT 250 WORDS)
Kudo
K
K
Department of Urology, Kyorin University School of Medicine.
Suwa
J
J
D000818:Animals; D002085:Butylhydroxybutylnitrosamine; D016516:CD4-CD8 Ratio; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D014810:Vitamin E / Q000493:pharmacokinetics / Q000494:pharmacology*; D014811:Vitamin E Deficiency / Q000276:immunology / Q000473:pathology
D014810:Vitamin E; D002085:Butylhydroxybutylnitrosamine