title: cDNA cloning of mitochondrial delta 3, delta 2-enoyl-CoA isomerase of rat liver.
abstract: A 1.08 kbp cDNA encoding rat liver mitochondrial delta 3, delta 2-enoyl-CoA isomerase (ECI) of 298 amino acid residues (Mr 32,895) was isolated from rat liver lambda gt11, lambda gt10 cDNA libraries by the combination of an immunochemical method with a rabbit-antibody against rat liver ECI and a plaque hybridization method. The deduced amino acid sequence from the cDNA indicates that ECI is synthesized with an amino-terminal extrasequence of 35 amino acid residues and processed to the mature enzyme (Mr 29,256).
authors.affiliation: Department of Pharmaceutical Sciences, Tohoku University Hospital, Miyagi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tomioka
authors.forename: A
authors.initials: A
authors.lastname: Hirose
authors.forename: H
authors.initials: H
authors.lastname: Moritani
authors.forename: T
authors.initials: T
authors.lastname: Hishinuma
authors.forename: T
authors.initials: T
authors.lastname: Hashimoto
authors.forename: M
authors.initials: M
authors.lastname: Mizugaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D019748:Carbon-Carbon Double Bond Isomerases; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D064006:Dodecenoyl-CoA Isomerase; D006801:Humans; D007535:Isomerases / Q000737:chemistry / Q000235:genetics*; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D051381:Rats; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D004247:DNA; D007535:Isomerases; D019748:Carbon-Carbon Double Bond Isomerases; D064006:Dodecenoyl-CoA Isomerase; C576298:ECI1 protein, human; C119738:ECI2 protein, human