Autologous anti-metatype immune response in rabbits.
Rabbits hyperimmunized with fluorescyl-conjugated KLH exhibited bound ligand associated with a high affinity circulating IgG anti-fluorescein population. After cessation of immunogen administration the liganded complexes were eventually spontaneously cleared from the circulation. Individual rabbits synthesized autologous anti-metatype antibodies specific for ligand-antibody complexes. Autologous anti-metatype antibodies reacted optimally with autologous liganded anti-fluorescein antibodies. However, cross reactivity was noted with allogenic rabbit liganded antibodies from three affinity-purified pools. An autologous anti-metatype response, reminiscent of autoanti-idiotype responses, has important implications concerning in vivo clearance of antigen-antibody complexes and may serve as a model to study immune complex diseases.
Voss
E W
EW
Department of Microbiology, University of Illinois, Urbana 61801.
Moore
J K
JK
Weidner-McGufficke
K M
KM
Denzin
L K
LK
Bedzyk
W D
WD
Voss
V H
VH
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000276:immunology* / Q000493:pharmacokinetics; D016650:Fluorescein-5-isothiocyanate / Q000276:immunology* / Q000378:metabolism; D006241:Haptens / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology* / Q000378:metabolism; D007110:Immunity, Active; D007074:Immunoglobulin G / Q000378:metabolism; D008024:Ligands; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D006241:Haptens; D007074:Immunoglobulin G; D008024:Ligands; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D016650:Fluorescein-5-isothiocyanate