Common double- and single-stranded DNA binding factor for a sterol regulatory element.
A cis-acting element necessary for sterol regulation, SRE-1, has previously been identified in the promoters of the low density lipoprotein receptor, hydroxymethylglutaryl (HMG)-CoA reductase, and HMG-CoA synthase genes. In this report we describe a nuclear factor, SRE-BF, isolated from Chinese hamster ovary nuclear extracts, that binds to the SRE-1 octanucleotide sequence. In addition to sequence-specific binding to SRE-1, as indicated by competition analysis with double-stranded DNA fragments, single-stranded oligomer DNA sequences also compete for binding in a sequence-specific fashion. Photochemical cross-linking experiments suggest that a common protein factor, with apparent molecular mass of 45-49 kDa, recognizes both single-stranded and double-stranded SRE-1. The binding specificity of SRE-BF to single-stranded SRE-1 closely correlates with the reported in vivo ability of SRE-1 to direct sterol responsiveness of transcription.
Stark
H C
HC
Department of Medicine, Columbia University, New York, NY 10032.
Weinberger
O
O
Weinberger
J
J
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D016466:CHO Cells; D002467:Cell Nucleus / Q000378:metabolism* / Q000528:radiation effects; D006224:Cricetinae; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D013261:Sterols / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*; D014466:Ultraviolet Rays
D015342:DNA Probes; D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D013261:Sterols