Metabolic alkalosis and the response of the trout, Salmo fario, to acute severe hypoxia.
Trout (Salmo fario) were acutely transferred from seawater to freshwater in order to induce blood metabolic alkalosis (cf. Maxime et al., J. Comp. Physiol. 160: 31-39, 1990). After 2 weeks, the fish were exposed to severe environmental hypoxia (final water oxygen partial pressure, PWO2 = 25-45 Torr, reached within 20 min), to assess the impact of the experimentally induced alkalosis, and hence increased haemoglobin-oxygen (Hb-O2) affinity, on various aspects of the hypoxic response. This was accomplished by monitoring oxygen partial pressure and total oxygen content of arterial blood (PaO2 and CaO2), extracellular pH (pHe), red blood cell (RBC) intracellular pH (pHi), and the concentrations of plasma adrenaline, noradrenaline, lactate and haemoglobin (Hb) at 5 min intervals. Blood from normoxic fish exhibited high pHe and RBC pHi values (8.32 +/- 0.02 and 7.53 +/- 0.03, respectively). During hypoxia PaO2 declined to 10 Torr within 25 min; the first 5 min provoked increases of pHe and pHi to 8.43 +/- 0.03 and 7.71 +/- 0.03, respectively; thereafter, pHe decreased whilst pHi remained elevated. The blood lactate concentration increased from 2.30 +/- 0.76 mmol.L-1 in normoxia to 14.94 +/- 5.7 mmol.L-1 at the conclusion (60 min) of the hypoxic exposure and catecholamine levels also increased progressively (from 2.94 +/- 0.51 and 1.90 +/- 0.50 nmol.L-1, in normoxia, to 191.91 +/- 64.25 and 72.00 +/- 25.02 nmol.L-1, at their highest levels, for adrenaline and noradrenaline, respectively). Determination of the PaO2 thresholds for lactate and catecholamine release demonstrated that these substances appeared in the bloodstream when the degree of O2 saturation of the haemoglobin fell below 60%. The results demonstrate that initial blood alkalosis does not prevent the typical physiological responses of trout to hypoxia but simply shifts to lower PaO2 values the threshold at which these responses begin.
Thomas
S
S
CNRS, UA 648, Faculté des Sciences et Techniques, Université de Bretagne Occidentale, Brest, France.
Perry
S F
SF
Pennec
Y
Y
Maxime
V
V
D000136:Acid-Base Equilibrium / Q000502:physiology*; D000818:Animals; D001784:Blood Gas Analysis; D002245:Carbon Dioxide / Q000378:metabolism*; D004837:Epinephrine / Q000097:blood; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007773:Lactates / Q000097:blood; D009638:Norepinephrine / Q000097:blood; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D014337:Trout
D007773:Lactates; D002245:Carbon Dioxide; D010100:Oxygen; D009638:Norepinephrine; D004837:Epinephrine