title: Control of acyl-CoA carboxylase activity in mycobacteria.
abstract: Acyl-CoA carboxylase activity in four pathogenic mycobacteria and Mycobacterium smegmatis was shown with both acetyl-CoA and propionyl-CoA substrates. Only very low activity was detected in mycobacteria grown in host tissues or on egg-based media rich in lecithin and avidin. This appeared to be a result of severe depression of activity, as strains which could be grown both in host tissue and egg-based media, and in the relatively simple Dubos or Sauton's media showed 8 to 120-fold higher activity in the simpler media.
authors.affiliation: Department of Applied Biology, University of Hull, U.K.
authors.forename: P R
authors.initials: PR
authors.lastname: Wheeler
authors.forename: K
authors.initials: K
authors.lastname: Bulmer
authors.forename: C
authors.initials: C
authors.lastname: Ratledge
authors.forename: J W
authors.initials: JW
authors.lastname: Dale
authors.forename: E
authors.initials: E
authors.lastname: Norman
mesh_terms: D000818:Animals; D019735:Carbon-Carbon Ligases; D003470:Culture Media; D008025:Ligases / Q000378:metabolism*; D051379:Mice; D009162:Mycobacterium avium / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity
chemical_list: D003470:Culture Media; D008025:Ligases; D019735:Carbon-Carbon Ligases; C018757:acyl-CoA carboxylase