Quiescence and proliferative response of normal human embryonic fibroblasts in homologous environment. Effect of aging.
A cell culture system for the study of human serum growth factors is described. It is based on a diploid human embryonic lung fibroblast cell strain (Flow 2002) with a finite life span (60 +/- 3 CPDs). When maintained in homologous (human) plasma-derived serum (PDS) at concentrations as low as 0.05%, the cells attain quiescence. Under these conditions, they remain viable for at least 14 days and they readily respond when stimulated to proliferate by human serum or human PDS, as well as by growth factors, such as PDGF, EGF, FGF. During in vitro aging, the cells retain their responsiveness to these growth stimuli, although the net proliferative effect decreases as they approach senescence.
Kletsas
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Laboratory for Enzyme Research, Institute of Biology, National Center for Scientific Research Demokritos, Athens, Greece.
Stathakos
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D001769:Blood; D002455:Cell Division; D002478:Cells, Cultured; D016922:Cellular Senescence; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004622:Embryo, Mammalian; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D005347:Fibroblasts; D006801:Humans; D007399:Interphase; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology
D003470:Culture Media; D016895:Culture Media, Serum-Free; D010982:Platelet-Derived Growth Factor; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor