title: Cloning and domain structure of the mammalian splicing factor U2AF.
abstract: A complementary DNA clone encoding the large subunit of the essential mammalian pre-messenger RNA splicing component U2 snRNP auxiliary factor (U2AF65) has been isolated and expressed in vitro. It contains two functional domains: a sequence-specific RNA-binding region composed of three ribonucleoprotein-consensus sequence domains, and an arginine/serine-rich motif necessary for splicing but not for binding to pre-mRNA.
authors.affiliation: Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
authors.forename: P D
authors.initials: PD
authors.lastname: Zamore
authors.forename: J G
authors.initials: JG
authors.lastname: Patton
authors.forename: M R
authors.initials: MR
authors.lastname: Green
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004722:Endoribonucleases / Q000235:genetics*; D005816:Genetic Complementation Test; D006801:Humans; D008322:Mammals; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D004247:DNA; D004722:Endoribonucleases; C022544:ribonuclease U2