Differential modulation by glucocorticoids of alternative complement protein secretion in cells of the monocyte/macrophage lineage.
The effect of the synthetic glucocorticoid dexamethasone (DXM) on the secretion by human monocytes of alternative complement proteins C3, factor B and factor H was investigated. Results indicated that DXM modulates this secretion in a direction which would be consistent with its anti-inflammatory properties. DXM, at therapeutic concentrations, had a suppressive effect on C3 and factor B secretion and a stimulatory effect on factor H secretion by monocytes. This differential modulation on C3, factor B and factor H secretion was similar in mature macrophages. Together with previous studies showing that DXM had a suppressive effect on C3 and factor B secretion and a stimulatory effect on factor H secretion by human endothelial cells, our results indicate that DXM appears to have the general property of regulating local production of complement components so as to control complement activation.
Lemercier
C
C
INSERM Unité 78, Bois-Guillaume, France.
Julen
N
N
Coulpier
M
M
Dauchel
H
H
Ozanne
D
D
Fontaine
M
M
Ripoche
J
J
D002454:Cell Differentiation; D002478:Cells, Cultured; D003176:Complement C3 / Q000378:metabolism*; D003180:Complement C3b Inactivator Proteins / Q000378:metabolism*; D011415:Complement Factor B / Q000378:metabolism*; D017242:Complement Factor H; D003170:Complement Pathway, Alternative; D003907:Dexamethasone / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins
C493495:CFH protein, human; D003176:Complement C3; D003180:Complement C3b Inactivator Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D003907:Dexamethasone; D017242:Complement Factor H; D007371:Interferon-gamma; D011415:Complement Factor B