Identification of replication and stability functions in the complete nucleotide sequence of plasmid pUH24 from the cyanobacterium Synechococcus sp. PCC 7942.
The complete nucleotide sequence is presented for pUH24, the small plasmid of Synechococcus sp. PCC 7942. pUH24 consists of 7835bp and has a G + C content of 59%. The distribution of translation start and stop codons in the sequence allows 36 open reading frames that potentially encode polypeptides of 50 or more amino acids. We postulate that eight of these open reading frames are actual coding sequences. A region has been identified, by experiment, that contains two functions, designated pmaA and pmaB, involved in the segregational stability of the plasmid. The minimal region of pUH24 fully capable of supporting autonomous replication consists of a 3.6kb DNA fragment, which is almost entirely occupied by two overlapping genes most likely coding for essential replication proteins (repA and repB).
Van der Plas
J
J
Department of Biotechnology, TNO Nutrition and Food Research, Zeist, The Netherlands.
Oosterhoff-Teertstra
R
R
Borrias
M
M
Weisbeek
P
P
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial