title: Analysis of a Nicotiana plumbaginifolia cDNA encoding a novel small GTP-binding protein.
abstract: Small GTP-binding proteins belonging to the Ras superfamily have been found in evolutionarily divergent organisms. Here, we report the isolation and analysis of a cDNA encoding a putative small GTP-binding protein, designated Rhn1, from the plant, Nicotiana plumbaginifolia. The 21.8-kDa protein has 60% amino acid similarity with the mammalian Rab5 proteins. The Rhn1 protein is encoded by a small multigene family. Northern analysis shows the highest steady-state mRNA levels to be in roots and flowers. Furthermore, the Rhn1 protein has 80% amino acid similarity with an Arabidopsis small GTP-binding protein, designated Rha1.
authors.affiliation: Laboratorium voor Genetica, Institut National de la Recherche Argonomique, Universiteit Gent, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Terryn
authors.forename: S
authors.initials: S
authors.lastname: Anuntalabhochai
authors.forename: M
authors.initials: M
authors.lastname: Van Montagu
authors.forename: D
authors.initials: D
authors.lastname: Inzé
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002454:Cell Differentiation; D019204:GTP-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D010947:Plants, Toxic; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics*
chemical_list: D019204:GTP-Binding Proteins