title: Reovirus genome segment assortment into progeny genomes studied by the use of monoclonal antibodies directed against reovirus proteins.
abstract: Using a panel of monoclonal antibodies (MABs) against reovirus proteins, we have identified proteins that associate with reovirus messenger RNA molecules prior to the generation of progeny double-stranded (ds) genome segments and proteins that are components of the structures within which progeny ds genome segments are generated. The following conclusions can be drawn from the results obtained. (1) Three proteins rapidly become associated with mRNA molecules to form single-stranded RNA-containing complexes (ssRCCs): the nonstructural protein microNS, the nonstructural protein sigma NS, and protein sigma 3. (2) Analysis of populations of ssRCCs in density gradients and by sequential exposure to various MABs indicates that some ssRCCs contain only microNS, others microNS and sigma NS or sigma 3, and others all three proteins. Each ssRCC contains one RNA molecule and, depending on the size of the RNA, 10-30 protein molecules. (3) The relative proportions of the individual RNA species in the ssRCC populations reflect the composition of the total mRNA population present in infected cells (which differs substantially from equimolarity). (4) RCCs that contain dsRNA, which become detectable as early as 4 hr after infection, contain not only microNS, sigma NS, and sigma 3, but also lambda 2. (5) The relative proportions of the 10 genome segments in dsRCCs are equimolar. This suggests that genome segment assortment into progeny genomes is linked to the transcription of plus strands into minus strands.
authors.affiliation: Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: J B
authors.initials: JB
authors.lastname: Antczak
authors.forename: W K
authors.initials: WK
authors.lastname: Joklik
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D006224:Cricetinae; D007739:L Cells; D051379:Mice; D008970:Molecular Weight; D011233:Precipitin Tests; D012367:RNA, Viral / Q000378:metabolism*; D012087:Reoviridae / Q000254:growth & development / Q000276:immunology / Q000648:ultrastructure*; D012261:Ribonucleoproteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D000911:Antibodies, Monoclonal; D012367:RNA, Viral; D012261:Ribonucleoproteins; D014764:Viral Proteins