title: Expression of c-fos protein in rat brain elicited by electrical stimulation of the pontine parabrachial nucleus.
abstract: The expression of Fos, the protein product of the primary response gene c-fos, was used metabolically to map the short-term (1 hr) effects of urethane and sodium pentobarbital anesthesia in rat. Subsequently, urethane-anesthetized rats were used to study the integrated response to electrical stimulation (1-1.5 hr) of the pontine parabrachial nucleus (PBN), an important center for relay of autonomic information in the brain. Immunohistochemistry was used to localize Fos-like immunoreactivity (FLI) in the brain. To approximate amounts of FLI in the conscious animal, rats were killed immediately after attaining surgical anesthesia with sodium pentobarbital (50 mg/kg) or urethane (1.2-1.7 gm/kg). No FLI was found in the brains of these rats. In rats killed 1 hr after anesthesia with sodium pentobarbital, FLI was found only in the habenulae. After 1 h of urethane anesthesia, low levels of FLI were found in the following areas: nucleus of the tractus solitarius (NTS); caudal and rostral ventrolateral medulla (VLM); lateral PBN; ventromedial, paraventricular, and supraoptic nuclei (SON) of the hypothalamus; medial preoptic area; central nucleus of the amygdala (ACE); endopiriform cortex; insular cortex; piriform cortex; and islands of Calleja. Electrical stimulation of the PBN (10 sec on, 10 sec off; 15-50 microA at 20 Hz for 60-90 min) in rats anesthetized with urethane led to increases in mean arterial pressure (10-30 mm Hg) and to ipsilateral increases of FLI in the lateral PBN, dorsal division of SON, ACE, endopiriform nucleus, insular cortex, piriform cortex, and islands of Calleja. In two animals, ipsilateral increases were found in the ventromedial hypothalamus and medial amygdaloid nucleus.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Alberta, Edmonton, Canada.
authors.forename: T L
authors.initials: TL
authors.lastname: Krukoff
authors.forename: T L
authors.initials: TL
authors.lastname: Morton
authors.forename: K H
authors.initials: KH
authors.lastname: Harris
authors.forename: J H
authors.initials: JH
authors.lastname: Jhamandas
mesh_terms: D000758:Anesthesia; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004558:Electric Stimulation; D007124:Immunoenzyme Techniques; D008297:Male; D010424:Pentobarbital; D011149:Pons / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014520:Urethane
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D014520:Urethane; D010424:Pentobarbital