Molecular neuroanatomy of monoamine oxidases in human brainstem.
Specific, high-resolution techniques (quantitative enzyme radioautography and in situ hybridisation histochemistry) have revealed distribution, abundance and cellular localization of the isoenzymes MAO-A and MAO-B and their mRNAs in human post-mortem brainstem. Whereas MAO-A protein and mRNA are expressed by noradrenergic neurons of the locus coeruleus, MAO-B protein and mRNA are expressed by serotoninergic neurons of the raphé nuclei. In the substantia nigra, MAO-B was more abundant than MAO-A; the former was localized in the reticular zone and the latter in the compact zone (where melanin-containing dopaminergic neurons are found). To date, it has not been possible to detect mRNA for either MAO-A or MAO-B in the substantia nigra or in glial cells of the brain regions investigated, suggesting either that the technique has limited sensitivity, or the possible existence of MAO-A and MAO-B subtypes.
Richards
J G
JG
Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd, Basel, Switzerland.
Saura
J
J
Ulrich
J
J
Da Prada
M
M
D000368:Aged; D001345:Autoradiography; D001933:Brain Stem / Q000033:anatomy & histology* / Q000201:enzymology; D002874:Chromosome Mapping; D005260:Female; D006801:Humans; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010848:Picolinic Acids / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D013844:Thiazoles / Q000494:pharmacology
D008996:Monoamine Oxidase Inhibitors; D015345:Oligonucleotide Probes; D010848:Picolinic Acids; D012333:RNA, Messenger; D013844:Thiazoles; C063456:Ro 41-1049; C059303:lazabemide; D008995:Monoamine Oxidase