Immunoreactive thyroliberin (TRH) precursor forms in human hypothalamus and anterior pituitary tissues.
Immunoreactive (IR) proTRH forms were characterized in human hypothalamic tissue with two antisera raised against a hepta- and a decapeptide containing the TRH progenitor sequence (-Gln-His-Pro-Gly-). A similar study was performed in human normal and adenomatous anterior pituitaries, tissues in which TRH synthesis has been previously suggested. IR-proTRH was found in all the samples ranging from 42-775 fmol/mg proteins. Size exclusion chromatography identified a major 25-35 kDa form and a minor 4-8 kDa form. The existence of the major form was confirmed by immunoblotting. The results suggest that both human hypothalamic and normal or adenomatous anterior pituitary tissues synthesize similar IR-proTRH forms.
Croissandeau
G
G
INSERM U223, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
Pagésy
P
P
Grouselle
D
D
Le Dafniet
M
M
Peillon
F
F
Li
J Y
JY
D000236:Adenoma / Q000378:metabolism; D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D006801:Humans; D007031:Hypothalamus / Q000737:chemistry*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010903:Pituitary Gland, Anterior / Q000737:chemistry*; D010911:Pituitary Neoplasms / Q000378:metabolism; D011498:Protein Precursors / Q000032:analysis*; D011863:Radioimmunoassay; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis*
D011498:Protein Precursors; D013973:Thyrotropin-Releasing Hormone