title: A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes.
abstract: Monoclonal antibody (mAb) G7 has been developed and appears to recognize a triggering structure on porcine natural killer (NK) cells and granulocytes. G7 mAb binds to approximately 13% of lymphocytes, 70% of monocytes, and greater than 95% of granulocytes. G7 mAb does not react with B cells. G7 mAb immunoprecipitates a heterodispersed molecule of approximately 40 kDa. Functionally, whole but not F(ab')2 fragments of G7 mAb enhance NK killing of Fc receptor positive K562, U937, and MOLT-4 targets but not Fc receptor negative CEM, WEHI-164, or YAC-1 targets. Both whole and F(ab')2 fragments of G7 mAb inhibit lymphocyte-mediated antibody-dependent cellular cytotoxicity. Interestingly, G7 mAb induces dramatic levels of granulocyte killing against nucleated K562 targets. These results suggest that G7 mAb recognizes a trigger molecule involved in porcine cellular cytotoxicity.
authors.affiliation: Department of Microbiology and Immunology, University of Health Sciences, Chicago Medical School, Illinois 60064.
authors.forename: M E
authors.initials: ME
authors.lastname: Dato
authors.forename: W G
authors.initials: WG
authors.lastname: Wierda
authors.forename: Y B
authors.initials: YB
authors.lastname: Kim
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D002460:Cell Line; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D011233:Precipitin Tests; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments