title: Studies on human lens: I. Origin and development of fluorescent pigments.
abstract: Fluorescence spectra of normal mature human lenses have been measured and at least eight species with distinct emission characteristics identified. To determine the specific photochemical and photophysical processes responsible for the origin and development of these fluorophores, emission behavior of the products generated by successive irradiation of young human lenses (3-6 y old) as well as of L-tryptophan solution have been systematically monitored. Fluorescent products that resulted from this irradiation were comparable to many of the fluorophores detected in aged lenses, indicating that light plays a major role in the development of these pigments. In addition to photogenerated species, there are other compounds in human lenses, presumably advanced glycosylated end products, with marked fluorescence properties. Several oxidation products of tryptophan including N-formylkynurenine or its derivatives, beta-carboline or its derivatives, and anthranilic acid have been identified in the mature human lens. The development of several photoproducts also was attributed to endogenous ascorbate-mediated Maillard reaction products, which undergo photoconversion by the visible light. Although some of these chromophores could act as photosensitizers, the sensitizing efficiency of many are low. Conversely, the near-UV filtering capability of these colored compounds conceivably could protect the vitreous and retina from development of any photochemical lesion.
authors.affiliation: Eye Research Institute, Harvard Medical School, Boston, MA 02114.
authors.forename: A C
authors.initials: AC
authors.lastname: Sen
authors.forename: N
authors.initials: N
authors.lastname: Ueno
authors.forename: B
authors.initials: B
authors.lastname: Chakrabarti
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002675:Child, Preschool; D006801:Humans; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000737:chemistry*; D008875:Middle Aged; D010777:Photochemistry; D010860:Pigments, Biological / Q000737:chemistry* / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000737:chemistry / Q000528:radiation effects
chemical_list: D010860:Pigments, Biological; D014364:Tryptophan