Isolation and characterisation of a new hepatic bilirubin UDP-glucuronosyltransferase. Absence from Gunn rat liver.
A novel UDP-glucuronosyltransferase that conjugates bilirubin IX alpha, bilirubin monoglucuronide and an arylalkanoic acid was purified to homogeneity from clofibrate treated Wistar rats. The enzyme displayed a subunit molecular mass of 54 kDa, a pI of 7.6 and was demonstrated to be N-glycosylated. Sequence analysis of peptides derived by endoproteinase Glu-C cleavage of the purified enzyme indicated that it was a new member of the recently identified UGT1 subfamily. Immunoblot analysis demonstrated that this enzyme was absent from Gunn rat liver. The molecular derivation of this enzyme and the lack of it in Gunn rats is discussed.
Clarke
D J
DJ
Department of Biochemical Medicine, University of Dundee, Ninewells Hospital and Medical School, UK.
Keen
J N
JN
Burchell
B
B
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D014453:Glucuronosyltransferase; D006602:Hexosyltransferases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D051381:Rats; D011911:Rats, Gunn; D011919:Rats, Inbred Strains
C019787:bilirubin glucuronoside glucuronosyltransferase; D006602:Hexosyltransferases; D014453:Glucuronosyltransferase