Yellow fever 17DD vaccine virus is temperature sensitive when grown in mosquito C6-36 cells.
Twenty-three yellow fever (YF) vaccine viruses and three wild-type YF viruses were propagated independently in human adenocarcinoma (SW13) cells and mosquito Aedes albopictus C6-36 cells. The three YF 17DD vaccine viruses were found to be slightly temperature sensitive (ts) at 39.5 degrees C versus 37.0 degrees C (efficiency of plaquing 0.04 to 0.1) following propagation in C6-36 but not in SW13 cells. A plaque-purified preparation of the 17DD vaccine manufactured in Brazil was ts when grown in C6-36 cells and remained ts when passaged back into the SW13 cell line.
Ledger
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TN
Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, UK.
Sil
B K
BK
Dunster
L M
LM
Stephenson
J R
JR
Minor
P D
PD
Barrett
A D
AD
D000330:Aedes / Q000382:microbiology*; D000818:Animals; D002460:Cell Line; D006358:Hot Temperature; D006801:Humans; D010641:Phenotype; D014407:Tumor Cells, Cultured; D014765:Viral Vaccines / Q000032:analysis*; D015005:Yellow fever virus / Q000235:genetics / Q000254:growth & development*
D014765:Viral Vaccines