title: The nifU, nifS and nifV gene products are required for activity of all three nitrogenases of Azotobacter vinelandii.
abstract: Strains with mutations in 23 of the 30 genes and open reading frames in the major nif gene cluster of A. vinelandii were tested for ability to grow on N-free medium with molybdenum (Nif phenotype), with vanadium (Vnf phenotype), or with neither metal present (Anf phenotype). As reported previously, nifE, nifN, nifU, nifS and nifV mutants were Nif- (failed to grow on molybdenum) while nifM mutants were Nif-, Vnf- and Anf-. nifV, nifS, and nifU mutants were found to be unable to grow on medium with or without vanadium, i.e. were Vnf- Anf-. Therefore neither vnf nor anf analogoues of nifU, nifS, nifV or nifM are expected to be present in A. vinelandii.
authors.affiliation: AFRC Institute of Plant Science Research, University of Sussex, Brighton, UK.
authors.forename: C
authors.initials: C
authors.lastname: Kennedy
authors.forename: D
authors.initials: D
authors.lastname: Dean
mesh_terms: D016948:Azotobacter vinelandii / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D005798:Genes, Bacterial; D005838:Genotype; D008982:Molybdenum / Q000494:pharmacology; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000235:genetics*; D010641:Phenotype; D014639:Vanadium / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; C088637:NifU protein, Bacteria; D014639:Vanadium; D008982:Molybdenum; D009591:Nitrogenase