Recombinant alpha-interferon inhibits colony formation of bone marrow fibroblast progenitor cells (CFU-F).
Alpha-Interferon (IFN-alpha) has been shown to inhibit colony formation of hematopoietic progenitor cells, including colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM), day 7 colony-forming unit granulocyte-macrophage (CFU-GM), day 14 CFU-GM, burst-forming unit erythroid (BFU-E), pluripotent stem cells (CFU-S), and colony-forming unit megakaryocyte (CFU-MK). The present study was designed to see whether IFN-alpha also has inhibitory effects on bone marrow fibroblast progenitors (CFU-F). We found that IFN-alpha exerted a significant inhibitory effect on both rabbit and human CFU-F formation. Inhibition of human marrow CFU-F formation by alpha interferon was unaffected by removal of 98% of monocytes/macrophages and T lymphocytes from light density marrow cells. This finding suggests that IFN-alpha probably exerts a direct inhibitory effects.
Wang
J C
JC
Department of Medicine, Brookdale Hospital Medical Center, New York 11212.
Lang
H D
HD
Liao
P
P
Wong
A
A
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001853:Bone Marrow / Q000737:chemistry / Q000187:drug effects; D001854:Bone Marrow Cells; D017252:CD3 Complex; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004950:Esterases / Q000032:analysis; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D007150:Immunohistochemistry; D007370:Interferon Type I / Q000494:pharmacology*; D008264:Macrophages / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D009000:Monocytes / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D011817:Rabbits; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000187:drug effects
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D007370:Interferon Type I; D011948:Receptors, Antigen, T-Cell; D011994:Recombinant Proteins; D004950:Esterases