Analysis of a Nicotiana plumbaginifolia cDNA encoding a novel small GTP-binding protein.
Small GTP-binding proteins belonging to the Ras superfamily have been found in evolutionarily divergent organisms. Here, we report the isolation and analysis of a cDNA encoding a putative small GTP-binding protein, designated Rhn1, from the plant, Nicotiana plumbaginifolia. The 21.8-kDa protein has 60% amino acid similarity with the mammalian Rab5 proteins. The Rhn1 protein is encoded by a small multigene family. Northern analysis shows the highest steady-state mRNA levels to be in roots and flowers. Furthermore, the Rhn1 protein has 80% amino acid similarity with an Arabidopsis small GTP-binding protein, designated Rha1.
Terryn
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N
Laboratorium voor Genetica, Institut National de la Recherche Argonomique, Universiteit Gent, Belgium.
Anuntalabhochai
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S
Van Montagu
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M
Inzé
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D
D000595:Amino Acid Sequence; D001483:Base Sequence; D002454:Cell Differentiation; D019204:GTP-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D010947:Plants, Toxic; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics*
D019204:GTP-Binding Proteins