Three C-phycoerythrin-associated linker polypeptides in the phycobilisome of green-light-grown Calothrix sp. PCC 7601 (cyanobacteria).
Microanalyses by SDS-PAGE and microsequencing demonstrate that, under green-light conditions, 3 C-phycoerythrin associated rod-linker polypeptides with different N-terminal amino acid sequences are present in phycobilisomes (PBS) from Calothrix sp. 7601 cells. Two of these polypeptides, corresponding to SDS-PAGE bands at 36 and 37 kDa, could be assigned, respectively, to the cpeC and cpcD genes found on a separate cpeCD-operon in Calothrix sp. 7601 (Federspiel, N.A. and Grossman, A.R. (1990) J. Bacteriol, 172, 4072-4081). The third C-PE rod-linker polypeptide, LR,2PE,33, requires, therefore, a third gene with the suggested locus designation 'cpeE'. A C-PE (alpha beta)6-LR,2PE,33 complex containing this third rod-linker polypeptide could be isolated from phycobilisomes and characterized. PBS from both green- and red-light cells of Calothrix contain a single, unique LRC28 rod-core linker polypeptide which is not altered during chromatic adaptation.
Glauser
M
M
Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule, Zürich, Switzerland.
Sidler
W A
WA
Graham
K W
KW
Bryant
D A
DA
Frank
G
G
Wehrli
E
E
Zuber
H
H
D000595:Amino Acid Sequence; D000458:Cyanobacteria / Q000378:metabolism* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009876:Operon; D010455:Peptides / Q000378:metabolism*; D045524:Phycobilisomes; D010799:Phycoerythrin / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
D010455:Peptides; D045524:Phycobilisomes; D010799:Phycoerythrin