title: Nonrandom F-plasmid replication in Escherichia coli K-12.
abstract: Both the autonomous and chromosomally integrated F plasmids were found to replicate in a nonrandom fashion after a density transfer from heavy medium ([13C]glucose, 15NH4) to light medium ([12C]glucose, 14NH4). The data are consistent with the hypothesis that both the chromosome and the F plasmid are replicated in a cell cycle-specific manner. Thus, these data support the proposal (J. D. Keasling, B. O. Palsson, and S. Cooper, J. Bacteriol. 173:2673-2680, 1991) that plasmids replicate in a cell cycle-specific manner.
authors.affiliation: Department of Microbiology, University of Uppsala Biomedical Center, Sweden.
authors.forename: L J
authors.initials: LJ
authors.lastname: Koppes
mesh_terms: D002453:Cell Cycle; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial