title: Solid phase enzyme immunoassays for the quantification of serum amyloid P (SAP) and complement component 3 (C3) proteins in acute-phase mouse sera.
abstract: Simple, reliable and sensitive enzyme immunoassays have been developed for the quantification of the mouse acute-phase SAP and C3 proteins. The ELISA systems were validated using sera from mice injected with S. dysenteriae endotoxin, and detected 500 pg protein/ml. The assays use 96-well microtitre plates permitting rapid processing of a large number of samples.
authors.affiliation: National Institute for Biological Standards and Control, Division of Endocrinology, South Mimms, Potters Bar, Herts, United Kingdom.
authors.forename: Y S
authors.initials: YS
authors.lastname: Taktak
authors.forename: B
authors.initials: B
authors.lastname: Stenning
mesh_terms: D000210:Acute-Phase Reaction / Q000097:blood*; D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity; D003176:Complement C3 / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000683:Serum Amyloid P-Component / Q000032:analysis*; D022603:Shiga Toxins; D012762:Shigella dysenteriae
chemical_list: D001427:Bacterial Toxins; D003176:Complement C3; D000683:Serum Amyloid P-Component; D022603:Shiga Toxins