Comparison of lysis of bromelain and papain treated red cells in ADCC assays.
Red cells were pretreated with the proteolytic enzymes bromelain or papain prior to use in antibody-dependent cell-mediated cytotoxicity (ADCC) assays with lymphocytes or peripheral blood mononuclear cells (PBMC) as effector cells. At low concentrations of anti-D or anti-A, lysis of papain-treated cells by lymphocytes was greater than that of bromelain-treated cells. Papain digestion resulted in both greater sensitivity to haemolysis by lymphocytes or PBMC and higher agglutination titres of anti-D-sensitised red cells than bromelain. With anti-A, however, although papain also promoted greater haemolysis, it was slightly less effective at red cell agglutination than bromelain.
Kumpel
B M
BM
U.K. Transplant Support Services Authority, Bristol.
Bakács
T
T
D000017:ABO Blood-Group System / Q000276:immunology; D000371:Agglutination / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D001963:Bromelains / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000276:immunology; D006461:Hemolysis / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D010206:Papain / Q000494:pharmacology*; D012204:Rh-Hr Blood-Group System / Q000276:immunology
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen; D001963:Bromelains; D010206:Papain