Incomplete process of recombinant human platelet-derived growth factor produced in yeast and its effect on the biological activity.
Partially purified recombinant human Platelet-derived Growth Factor BB homodimer isolated from yeast culture media contains variable amounts of unprocessed PDGF-BB. This unprocessed PDGF-BB is found as a result of incomplete cleavage of the precursor to form the mature protein. Although the signal peptide is efficiently removed, a fraction of the PDGF secreted has an extended sequence corresponding to the truncated yeast alpha-factor leader. The data suggest that it is the amino acid chain from the truncated a-factor leader and not the sugar moiety attached to it that is responsible for the higher mitogenic activity found in this unprocessed molecule compared to highly purified PDGF-BB.
Calderón-Cacia
M
M
Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608.
Tekamp-Olson
P
P
Allen
J
J
George-Nascimento
C
C
D000595:Amino Acid Sequence; D015153:Blotting, Western; D006031:Glycosylation; D006801:Humans; D046911:Macromolecular Substances; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010982:Platelet-Derived Growth Factor / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D021382:Protein Sorting Signals / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013329:Structure-Activity Relationship
D046911:Macromolecular Substances; D008934:Mitogens; D010982:Platelet-Derived Growth Factor; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011994:Recombinant Proteins