Alloantisera can detect some of the variants of HLA DRw13 identified by oligotyping.
Oligotyping has revealed a considerable polymorphism of HLA DRw13; so far, 5 alleles coded by DRB1 have been identified. An even greater number of haplotypes are apparent when considering the association of DRw13 with alleles coded by DRB3 and DQB1. Serology can now define some of these variants which had formerly escaped detection. We have tested by serology and by oligoprobes 66 genotyped Caucasoid cells comprising the known DR and DQ alleles. Among the 28 DRw6 cells, 10 different haplotype patterns were selected, of which 8 concern DRw13 and 2 DRw14. Four variants of DRw13 were represented: DRB1 *1301, *1302, *1303 and *1305 associated with usual and with uncommon alleles belonging to DRB3 and to DQB1. We have centered our analysis on 19 sera of the XIth Workshop defining DRw6. Two sera (639, 826) were noteworthy since they reacted with certain subtypes of DRw13 and with DRB1 *1102, *1103 only, the other DR specificities remaining negative.
Gebuhrer
L
L
Histocompatibility Laboratory, Blood Transfusion Center, Lyon, France.
Lambert
J
J
Labonne
M P
MP
Mollet
I
I
Freidel
A C
AC
Betuel
H
H
D000595:Amino Acid Sequence; D015343:DNA Probes, HLA / Q000235:genetics; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology*; D059810:HLA-DR Serological Subtypes; D006239:Haplotypes; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D016130:Immunophenotyping / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D044465:White People / Q000235:genetics
D015343:DNA Probes, HLA; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C097337:HLA-DR13 antigen; D007106:Immune Sera