title: Reappraisal of the hypo-osmotic swelling test to improve assessment of seminal fertility status.
abstract: The hypo-osmotic swelling (HOS) test has been proposed as a useful assay for evaluation of the functional competence of the human sperm membranes. To assess this further, the HOS-test was evaluated in 187 semen samples collected from fertile men and from male patients consulting for infertility. These samples were classified as normal, oligo-, astheno- or oligoasthenozoospermic on the basis of their standard semen variables. The percentage of total sperm tail swelling and of sperm exhibiting different tail swelling patterns was recorded. In the fertile men and in the group of patients with normal semen variables, significantly more (P less than 0.001) HOS-reactive sperm were observed after hypo-osmotic treatment in comparison with those groups exhibiting abnormal semen parameters. Swelling of the sperm in a hypo-osmotic medium was highly correlated with both progressive motility (r = 0.62, P less than 0.001) and sperm viability (r = 0.65, P less than 0.001). A weak positive correlation was also observed between sperm swelling and sperm morphological features (r = 0.31, P less than 0.005) and between sperm swelling and sperm concentration (r = 0.31, P less than 0.005). No significant correlation was observed between sperm swelling and in-vitro sperm fertilizing capacity as assessed by the zona-free hamster oocyte penetration assay. However the majority of the semen samples (87.3%) showing a normal penetration rate (greater than or equal to 10%) also exhibited a 60% (or higher) reaction in the HOS-test.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Cell Biology and Genetics, School of Medicine, University of Chile, Santiago.
authors.forename: R
authors.initials: R
authors.lastname: Smith
authors.forename: M
authors.initials: M
authors.lastname: Madariaga
authors.forename: E
authors.initials: E
authors.lastname: Bustos-Obregon
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000473:pathology; D006224:Cricetinae; D005069:Evaluation Studies as Topic; D005260:Female; D007248:Infertility, Male / Q000175:diagnosis*; D008297:Male; D009997:Osmotic Pressure; D012661:Semen; D013076:Sperm Count; D013081:Sperm Motility; D013082:Sperm Tail / Q000473:pathology; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000473:pathology