title: The products of the kdpDE operon are required for expression of the Kdp ATPase of Escherichia coli.
abstract: The expression of the Kdp system for K+ uptake in Escherichia coli requires the products of two genes, kdpD and kdpE. These genes constitute an operon adjacent to the kdpABC operon that encodes the three membrane protein subunits of Kdp. Both operons are transcribed in the same direction and overlap; the kdpDE promoter is in kdpC, the last gene of the kdpABC operon. Transcription of the kdpDE operon is at a low level when Kdp is not expressed; transcription increases about 10-fold when kdpABC is turned on, indicating significant read-through of the kdpDE operon by transcripts beginning at the promoter of kdpABC operon. The proximal region of the kdpD gene is the site of most mutations that lead to constitutive expression of the kdpABC operon.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637.
authors.forename: J W
authors.initials: JW
authors.lastname: Polarek
authors.forename: G
authors.initials: G
authors.lastname: Williams
authors.forename: W
authors.initials: W
authors.lastname: Epstein
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001693:Biological Transport, Active; D027682:Cation Transport Proteins; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D009876:Operon; D011188:Potassium / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D004269:DNA, Bacterial; D000251:Adenosine Triphosphatases; C022761:potassium transporting ATPase; D011188:Potassium