Sucrose promotes the functional activity of blood vessels after cryopreservation in DMSO-containing fetal calf serum.
The cryoprotective effect of sucrose has been investigated using 3 different vascular smooth muscle preparations, namely canine saphenous veins and arteries and porcine circumflex coronary arteries following storage in liquid nitrogen (at -196 degrees C). Contractile responses to noradrenaline, 5-HT, prostaglandin F2 alpha and KCl and relaxant responses to substance P and 5-HT were determined on fresh tissues and after cryostorage in fetal calf serum (FCS) containing either 1.8 M dimethyl sulfoxide (DMSO), or 0.1 M sucrose or both agents combined. The data demonstrate that the addition of sucrose to the DMSO-containing cryomedium promotes the preservation of both contractile and relaxant activity of cryostored blood vessels, though sucrose alone did not confer any noticeable protection.
Müller-Schweinitzer
E
E
Preclinical Research, Sandoz Pharma AG, Basel, Switzerland.
Ellis
P
P
D000818:Animals; D001808:Blood Vessels / Q000187:drug effects* / Q000502:physiology; D002417:Cattle; D015925:Cryopreservation; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage; D009638:Norepinephrine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D012501:Saphenous Vein / Q000187:drug effects / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology*
D012701:Serotonin; D013395:Sucrose; D011189:Potassium Chloride; D009638:Norepinephrine; D004121:Dimethyl Sulfoxide