Homology of the root adhesin of Pseudomonas fluorescens OE 28.3 with porin F of P. aeruginosa and P. syringae.
The gene encoding the root adhesin from the outer membrane of Pseudomonas fluorescens OE 28.3 was isolated from a genomic lambda EMBL3 library and sequenced. The deduced protein (32104 daltons) displayed strong homology with the amino- and carboxyterminal parts of porin F (OprF) from P. aeruginosa and P. syringae. Significant homology was also found within the C-terminal domain of the OmpA proteins from Enterobacteria and major outer membrane proteins from Neisseria species. However, a cysteine-rich domain present in the OprFs of P. aeruginosa and P. syringae is absent from the adhesin of P. fluorescens. Instead, it contains a shorter sequence with eight alternating proline residues.
De Mot
R
R
F.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium.
Proost
P
P
Van Damme
J
J
Vanderleyden
J
J
D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D015723:Gene Library; D005800:Genes, Fungal; D037102:Lectins; D008969:Molecular Sequence Data; D018272:Porins; D011549:Pseudomonas / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D011551:Pseudomonas fluorescens / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D037102:Lectins; D018272:Porins; C038692:adhesin, Pseudomonas