Defining the carbohydrate specificities of Abrus precatorius agglutinin as T (Gal beta 1----3GalNAc) greater than I/II (Gal beta 1----3/4GlcNAc).
The combining site of the nontoxic carbohydrate binding protein (Abrus precatorius agglutinin, APA) purified from the needs of Abrus precatorius (Jequirity bean), was studied by quantitative precipitin and precipitin-inhibition assays. Of 26 glycoproteins and polysaccharides tested, all, except sialic acid-containing glycoproteins and desialized ovine salivary glycoproteins, reacted strongly with the lectin, and precipitated over 70% of the lectin added, indicating that APA has a broad range of affinity and recognizes (internal) Gal beta 1----sequences of carbohydrate chains. The strong reaction with desialized porcine and rat salivary glycoproteins as well as pneumococcus type XIV polysaccharide suggests that APA has affinity for one or more of the following carbohydrate sequences: Thomsen-Friedenreich (T, Gal beta 1----3GalNAc), blood group precursor type I and/or type II (Gal beta 1----3/4GlcNAc) disaccharide determinants of complex carbohydrates. Among the oligosaccharides tested, the T structure was the best inhibitor; it was 2.4 and 3.2 times more active than type II and type I sequences, respectively. The blood group I Ma-active trisaccharide, Gal beta 1----4GlcNAc beta 1----6Gal, was about as active as the corresponding disaccharide (II). From the above results, we conclude that the size of the combining site of the A. precatorius agglutinin is probably as large as a disaccharide and most strongly complementary to the Gal beta 1----3GalNAc (T determinant) sequence. The carbohydrate specificities of this lectin will be further investigated once the related oligosaccharide structures become available.
Wu
A M
AM
Glyco-Immunochemistry Research Laboratory, Chang-Gung Medical College, Tao-yuan, Taiwan.
Lin
S R
SR
Chin
L K
LK
Chow
L P
LP
Lin
J Y
JY
D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism; D002240:Carbohydrate Sequence; D004492:Edetic Acid / Q000494:pharmacology; D007887:Fabaceae / Q000737:chemistry*; D006023:Glycoproteins / Q000378:metabolism; D006384:Hemagglutination; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D037121:Plant Lectins; D010946:Plants, Medicinal; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D013329:Structure-Activity Relationship
D006023:Glycoproteins; D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; D011135:Polysaccharides, Bacterial; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium