title: Immunohistochemical detection of c-myc products in colorectal cancer and proliferative cell rate.
abstract: Expression of c-myc protein was studied immunohistochemically in colorectal cancers using a monoclonal antibody, MYC-1. Immunoblotting assays with cellular lysates demonstrated a band of the gene products at the level of 60 kDa. c-myc-protein-positive tumor cells were observed in 43 (43.4%) of 99 specimens of colorectal cancers. There was no significant correlation between the incidence of MYC-1-positive tumors and clinicopathological findings. The rate of MYC-1 proteins occurring in patients with liver metastasis was significantly higher than that in patients without the metastasis. The rate of occurrence of DNA polymerase-alpha-positive cells in MYC-1 protein-positive tumors was significantly higher than in MYC-1 negative ones. The results suggested that MYC-1 immunoreactivity might possibly be a useful prognostic marker of colorectal cancers.
authors.affiliation: Department of Surgery II, School of Medicine, Kanazawa University, Ishikawa, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: I
authors.initials: I
authors.lastname: Ninomiya
authors.forename: T
authors.initials: T
authors.lastname: Ishida
authors.forename: G
authors.initials: G
authors.lastname: Nishimura
authors.forename: M
authors.initials: M
authors.lastname: Kanno
authors.forename: Y
authors.initials: Y
authors.lastname: Yonemura
authors.forename: K
authors.initials: K
authors.lastname: Miwa
authors.forename: I
authors.initials: I
authors.lastname: Miyazaki
authors.forename: S
authors.initials: S
authors.lastname: Matsukawa
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D015153:Blotting, Western; D002455:Cell Division / Q000235:genetics; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D004257:DNA Polymerase II / Q000032:analysis; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis*
chemical_list: D016271:Proto-Oncogene Proteins c-myc; D004257:DNA Polymerase II