Immunohistological localization of beta-2-microglobulin in renal tissue as an indicator of renal dysfunction.
To elucidate the relationship between localization of beta 2-microglobulin (beta 2-MG) and renal lesions or dysfunction, 119 patients with various renal diseases and various degrees of renal injuries were examined: patients with beta 2-MG deposition (group 1, n = 69), and patients without renal beta 2-MG deposition (group 2, n = 50). beta 2-MG was found mainly in the tubular epithelium and tubular casts. No significant difference in the degree of proteinuria and hematuria were found between the two groups. Group 1 had a significant decrease in glomerular filtration rate (GFR; p less than 0.01): the average values of GFR in group 1 and 2 were 61.1 +/- 35.7 and 95.4 +/- 34.5 ml/min. Group 1 had a significant decrease in the phenolsulfonphthalein excretion test (p less than 0.01) and the maximum urine specific gravity in Fishberg's concentration test (p less than 0.02). Group 1 had a significant high incidence of glomerular sclerotic lesions (p less than 0.001), arteriolar elastosis (p less than 0.01), tubulo-interstitial changes (p less than 0.001) and renal deposition of lysozyme (p less than 0.001). The present study demonstrates that the immunohistological study of renal beta 2-MG deposition is a reliable method to identify renal dysfunction and renal injuries, especially the presence of tubulo-interstitial changes, in various renal diseases.
Suzuki
S
S
Department of Medicine (II), Niigata University School of Medicine, Japan.
Sato
H
H
Inomata
A
A
Maruyama
H
H
Ueno
M
M
Gejyo
F
F
Arakawa
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D005455:Fluorescent Antibody Technique; D005919:Glomerular Filtration Rate; D006801:Humans; D007150:Immunohistochemistry; D007674:Kidney Diseases / Q000175:diagnosis* / Q000503:physiopathology / Q000652:urine; D007684:Kidney Tubules / Q000737:chemistry* / Q000503:physiopathology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D010637:Phenolsulfonphthalein / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000032:analysis*
D001613:beta 2-Microglobulin; D010637:Phenolsulfonphthalein