Delivery of an accessory signal for cell activation by exogenous phosphatidylinositol-specific phospholipase C.
Digestion of phosphatidylinositol (PI) or glycosylphosphatidylinositol (GPI) anchors of membrane proteins on the external cell surface with exogenous PI-specific phospholipase C (PIPLC) from Bacillus thuringiensis was shown to transmit a signal into the thymocyte to modulate the TCR/CD3 complex-induced signal delivery for cell activation. This was demonstrated for very early protein tyrosine phosphorylation, early c-fos transcription and late DNA synthesis. For this effect preincubation of the cells with PIPLC was required, but there was no evidence of involvement of any soluble products released from the cell surface by PIPLC in the signaling, suggesting a crucial role of the membrane-bound counterpart (diacylglycerol or diradylglycerol) of the PI/GPI hydrolysate. A possible role for this accessory signal in the microorganism-linked control of the (diacylglycerol or diradylglycerol) of the PI/GPI hydrolysate. A possible role for this accessory signal in the microorganism-linked control of the T cell receptor function is discussed.
Jamshedur Rahman
S M
SM
Department of Immunology, Nagoya University School of Medicine, Japan.
Pu
M V
MV
Zhang
Y H
YH
Hamaguchi
M
M
Iwamoto
T
T
Taguchi
R
R
Ikezawa
H
H
Isobe
K
K
Yoshida
T
T
Nakashima
I
I
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D001413:Bacillus thuringiensis / Q000201:enzymology; D017252:CD3 Complex; D002478:Cells, Cultured; D006017:Glycolipids / Q000378:metabolism; D017261:Glycosylphosphatidylinositols; D051379:Mice; D008810:Mice, Inbred C57BL; D010716:Phosphatidylinositols / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D015398:Signal Transduction; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism*
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D010716:Phosphatidylinositols; D011948:Receptors, Antigen, T-Cell; D010738:Type C Phospholipases