Aluminum(III) influences the permeability of the blood-brain barrier to [14C]sucrose in rats.
To determine the influence of the metal coordination sphere on the permeability of the blood-brain barrier (BBB), rats were injected intraperitoneally with aluminum lactate (Al(lact)3), aluminum acetylacetonate (Al(acac)3), aluminum maltolate (Al(malt)3) at pH 7.5, or with physiological saline. Two h after each treatment, [14C]sucrose physiological saline solution was injected in animals, and the radioactivity was measured in 5 brain regions (cerebral cortex, mesencephalon, diencephalon, medulla-pons, cerebellum). Radioactivity was significantly elevated in brains from animals treated with Al(malt)3 (hydrolytically stable and hydrophilic), and with Al(acac)3 (hydrolytically stable and lipophilic) but not with Al(lact)3. Time-course study carried out at 2, 4 and 24 h with different aluminum compounds showed a persistent radioactivity 24 h after treatment only in the brain from animals treated with Al(acac)3. Morin stain localized AlIII only in neurons from animals treated with Al(acac)3. These findings indicate that AlIII alters the BBB function in the rat either permanently or transiently depending on the physiochemical properties of the metal coordination sphere. Implications of these results, in terms of AlIII as a potential toxic factor in humans, are considered and discussed.
Favarato
M
M
CNR-Unit for the study of Physiology and Biochemistry of Hemocyanin and other Metalloproteins, Padua, Italy.
Zatta
P
P
Perazzolo
M
M
Fontana
L
L
Nicolini
M
M
D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D001921:Brain / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism*; D002250:Carbon Radioisotopes; D007773:Lactates / Q000494:pharmacology; D019344:Lactic Acid; D008297:Male; D009928:Organ Specificity; D009942:Organometallic Compounds / Q000494:pharmacology; D010422:Pentanones / Q000494:pharmacology; D011753:Pyrones / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012039:Regional Blood Flow / Q000187:drug effects; D013395:Sucrose / Q000378:metabolism*
D002250:Carbon Radioisotopes; D007773:Lactates; D009942:Organometallic Compounds; D010422:Pentanones; D011753:Pyrones; C067527:aluminum maltolate; D019344:Lactic Acid; D013395:Sucrose; C058508:tris(acetylacetonate) aluminum(III); D000535:Aluminum