Specific deuteration of dichlorobenzoate during reductive dehalogenation by Desulfomonile tiedjei in D2O.
Desulfomonile tiedjei DCB-1 is a strict anaerobe capable of reductively dechlorinating meta-chlorobenzoates. To probe the mechanism of this aryl dechlorination, we incubated cell suspensions of D. tiedjei in D2O and with 2,5-dichlorobenzoate. The deuterium was incorporated into the dechlorination product exclusively at the position of dehalogenation, as shown by gas chromatography-mass spectrometry and proton magnetic resonance analyses. These results favor a model for dechlorination that should not allow proton exchange at other positions, as would be the case if partial ring reduction occurred.
Griffith
G D
GD
Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824.
Cole
J R
JR
Quensen
J F
JF
Tiedje
J M
JM
D001421:Bacteria, Anaerobic / Q000378:metabolism*; D002723:Chlorobenzoates / Q000378:metabolism*; D003903:Deuterium / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry
D002723:Chlorobenzoates; D003903:Deuterium; C073069:2,5-dichlorobenzoic acid