Cytological and molecular characterization of centromeres in Mus domesticus and Mus spretus.
We have applied EM in situ hybridization (EMISH) and pulsed field gel electrophoresis (PFGE) to samples from diploid primary cell cultures and an established cell line to examine in detail the relative organization of the major and minor satellite DNAs and telomere sequences in the genomes of Mus domesticus and Mus spretus. EMISH localizes the Mus domesticus minor satellite to a single site at the centromere-proximal end of each chromosome. Double label hybridizations with both minor satellite and telomere probes show that they are in close proximity and possibly are linked. In fact, PFGE of M. domesticus DNA digested with Sal I and Sfi I reveals the presence of fragments which hybridize to both probes and is consistent with the physical linkage of these two sequences. The M. domesticus minor satellite is the more abundant satellite in Mus spretus. Its distribution in M. spretus is characterized by diffuse labeling with no obvious concentration near chromosome ends. In addition to this repeat the M. spretus genome contains a small amount of DNA that hybridizes to a M. domesticus major satellite probe. Unlike the M. domesticus minor satellite, it is not telomere proximal but is confined to a domain at the border of the centromere and the long arm. Thus, although both species possess all three sequences, except for the telomeres, their distribution relative to one another is not conserved. Based on the results presented, we propose preliminary molecular maps of the centromere regions of Mus domesticus and Mus spretus.
Narayanswami
S
S
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Doggett
N A
NA
Clark
L M
LM
Hildebrand
C E
CE
Weier
H U
HU
Hamkalo
B A
BA
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002503:Centromere / Q000648:ultrastructure*; D016521:Electrophoresis, Gel, Pulsed-Field; D051379:Mice; D008854:Microscopy, Electron; D009115:Muridae; D009693:Nucleic Acid Hybridization