title: Regeneration of pericardial tissue on absorbable polymer patches implanted into the pericardial sac. An immunohistochemical, ultrastructural and biochemical study in the sheep.
abstract: A new absorbable polymer prepared from polyhydroxybutyrate (PHB) was inserted as a pericardial patch in sheep to serve as a temporary scaffold for regeneration of pericardial tissue. Postoperative adhesions were rare or absent. The present study focuses on characterization of the regenerated surface cells. The luminal surface of the regenerated tissue was covered with a complete layer of mesothelium-like cells which at light and scanning electron microscopy resembled those in native pericardium. Immunohistochemical stainings for cytokeratin and thrombomodulin were positive in these cells. Heparan sulfate proteoglycan was found in a basement-membrane-like structure beneath the surface cells, as in the normal pericardium. Transmission electron microscopy of the regenerated surface revealed cells with the characteristics of mesothelium. Prostacyclin production in the regenerated tissue was similar to that in native pericardium. The results indicate regeneration of a mesothelial layer with many of the important functions of native mesothelial cells. This may explain the presently and previously observed prevention of pericardial adhesions after cardiac surgery in this field. Clinical testing of PHB patches as pericardial substitutes is warranted in cardiac surgery when pericardial closure is desired.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, University Hospital, Uppsala, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Malm
authors.forename: S
authors.initials: S
authors.lastname: Bowald
authors.forename: A
authors.initials: A
authors.lastname: Bylock
authors.forename: T
authors.initials: T
authors.lastname: Saldeen
authors.forename: C
authors.initials: C
authors.lastname: Busch
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D011464:Epoprostenol / Q000378:metabolism; D006885:Hydroxybutyrates; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D010496:Pericardium / Q000033:anatomy & histology / Q000502:physiology* / Q000601:surgery; D019736:Prostheses and Implants; D012038:Regeneration; D012756:Sheep
chemical_list: D006885:Hydroxybutyrates; D011464:Epoprostenol