title: Nonradioactive quantification of low concentrations of hemoglobin A by HPLC for midtrimester prenatal diagnosis of beta-thalassemia.
abstract: The usual methods for prenatal diagnosis of beta-thalassemia and other hemoglobinopathies by assay of fetal blood erythrocytes are either complex (analysis of globin chains synthesis by carboxymethylcellulose chromatography) or only semiquantitative [isoelectric focusing of hemoglobin (Hb)]. To further simplify the diagnostic procedure and to obtain quantitative data, we measured the small concentrations of Hb A in fetal erythrocytes by using a high-pressure liquid chromatography (HPLC) instrument (DIAMAT-TM; Bio-Rad) equipped with the new column proposed for measuring Hb A2. We analyzed 212 uncontaminated fetal blood samples obtained by cordocentesis between the 18th and 22nd weeks of pregnancy, using the HPLC procedure, and compared the results with those obtained by the above-named methods. The Hb A values obtained ranged between 0% and 8.5%; they were less than or equal to 1.8% in 44 fetuses affected by homozygous beta-thalassemia and greater than 2.5% in 168 unaffected fetuses. The method was simple, rapid, and reproducible (CV 3.2%) and there was good correlation between Hb A concentrations determined by HPLC and the beta/gamma ratio determined by carboxymethylcellulose chromatography (r = 0.7687; P less than 0.0001). No false-negative or false-positive results were observed, and there was no overlap of values between affected and unaffected fetuses.
authors.affiliation: Laboratorio di Ricerche Cliniche, Anatomia ed Istologia Patologica, Istituti Clinici di Perfezionamento, Milano, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Maiavacca
authors.forename: S
authors.initials: S
authors.lastname: Tedeschi
authors.forename: A
authors.initials: A
authors.lastname: Mosca
authors.forename: S
authors.initials: S
authors.lastname: Calmi
authors.forename: P
authors.initials: P
authors.lastname: De Leonardis
authors.forename: C
authors.initials: C
authors.lastname: Todaro
authors.forename: G
authors.initials: G
authors.lastname: Zuliani
authors.forename: M
authors.initials: M
authors.lastname: Buscaglia
authors.forename: A
authors.initials: A
authors.lastname: Cantù-Rajnoldi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005260:Female; D006441:Hemoglobin A / Q000032:analysis*; D006720:Homozygote; D006801:Humans; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011296:Prenatal Diagnosis; D013789:Thalassemia / Q000097:blood / Q000175:diagnosis* / Q000235:genetics
chemical_list: D006441:Hemoglobin A