Elevation of levels of carcinogenic tryptophan pyrolysis products in plasma and red blood cells of patients with uremia.
The carcinogenic tryptophan pyrolysis products, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), have been measured in plasma and red blood cells (RBC) of patients with uremia and normal subjects by using a high-performance liquid chromatography (HPLC) method. In both uremic patients and normal subjects, these carcinogens have been detected in RBC as well as plasma. Trp-P-1 and Trp-P-2 levels in plasma of uremic patients were significantly higher than those of normal subjects. Moreover, these carcinogen levels in RBC (per hemoglobin) were significantly elevated in uremic patients in spite of the presence of severe anemia. These results suggest that patients with uremia are continuously exposed to higher levels of these carcinogens as compared with normal subjects. Our data also support the idea that these carcinogens in plasma and RBC are suitable for monitoring exposure levels in humans.
Manabe
S
S
Department of Hygiene & Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Suzuki
M
M
Kusano
E
E
Wada
O
O
Asano
Y
Y
D000328:Adult; D000368:Aged; D002243:Carbolines / Q000097:blood* / Q000302:isolation & purification; D002273:Carcinogens / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000737:chemistry*; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009153:Mutagens / Q000032:analysis* / Q000302:isolation & purification; D010949:Plasma / Q000737:chemistry; D014511:Uremia / Q000097:blood*
D002243:Carbolines; D002273:Carcinogens; D006454:Hemoglobins; D009153:Mutagens; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole