title: The arsenical ATPase efflux pump mediates tellurite resistance.
abstract: The ars operon of the resistance plasmid R773 was found to produce moderate levels of resistance to tellurite. A MIC of 64 micrograms of TeO3(2-) per ml was found for Escherichia coli cells harboring plasmids which contained all three of the structural genes (arsA, arsB, and arsC) of the anion-translocating ATPase. MICs specified by plasmids carrying only one or two structural elements or the cloning vector alone were 2 to 4 micrograms/ml. The rate of TeO3(2-) uptake was found to be on the order of 55% less for cultures containing the resistance plasmids.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Canada.
authors.forename: R J
authors.initials: RJ
authors.lastname: Turner
authors.forename: Y
authors.initials: Y
authors.lastname: Hou
authors.forename: J H
authors.initials: JH
authors.lastname: Weiner
authors.forename: D E
authors.initials: DE
authors.lastname: Taylor
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000965:Antimony / Q000378:metabolism / Q000633:toxicity; D001149:Arsenates / Q000378:metabolism / Q000633:toxicity; D001151:Arsenic / Q000378:metabolism / Q000633:toxicity; D020261:Arsenic Poisoning; D001152:Arsenicals / Q000378:metabolism*; D053501:Arsenite Transporting ATPases; D018053:Arsenites; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D016623:Ion Pumps; D008565:Membrane Proteins / Q000378:metabolism; D009097:Multienzyme Complexes; D011815:R Factors / Q000235:genetics*; D013691:Tellurium / Q000378:metabolism* / Q000633:toxicity
chemical_list: D001149:Arsenates; D001152:Arsenicals; D018053:Arsenites; D016623:Ion Pumps; D008565:Membrane Proteins; D009097:Multienzyme Complexes; C061374:antimonite; D000965:Antimony; D000251:Adenosine Triphosphatases; D053501:Arsenite Transporting ATPases; C015001:arsenite; D001151:Arsenic; D013691:Tellurium