title: Geometric relationship between the nicotinamide and isoalloxazine rings in NADPH-cytochrome P-450 oxidoreductase: implications for the classification of evolutionarily and functionally related flavoproteins.
abstract: The stereospecificity of hydride abstraction from NADPH and the conformation of the nicotinamide ring around the glycosidic bond have been determined for the flavoprotein NADPH-cytochrome P-450 oxidoreductase (P-450R). The A-side (pro-R) hydrogen is abstracted from NADPH, and the nicotinamide ring is in the anti conformation. These results are consistent with the apparently strong correlation between A-side stereospecificity and anti conformation and between B-side stereospecificity and syn conformation [You, K. (1985) CRC Crit. Rev. Biochem. 17, 313]. This correlation reveals how the flavin and nicotinamide rings are oriented relative to each other. In P-450R, the flavin is then "on top of" (on the exo side of) the nicotinamide ring. In another flavoprotein dehydrogenase, glutathione reductase, which is a B-side/anti enzyme [Pai, E. F., & Schulz, G. E. (1983) J. Biol. Chem. 258, 1752], the flavin is "underneath" (on the endo side of) the nicotinamide ring. We argue that all enzymes that are evolutionarily related to these two flavoproteins should have their respective overall configurations. The overall configuration is defined by the following five properties: (1) relative orientation of the isoalloxazine and nicotinamide rings, (2) stereospecificity of hydride transfer to/from the nicotinamide ring, (3) conformation of the nicotinamide ring around the glycosidic bond, (4) stereospecificity of hydride transfer to/from the flavin, and (5) conformation of the flavin around its N5-N10 axis. There are only eight possible overall configurations, and a knowledge of only three of the five properties is needed to determine which one is present (as long as the combination of properties is not 1, 2, 3 or 1, 4, 5).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
authors.forename: D S
authors.initials: DS
authors.lastname: Sem
authors.forename: C B
authors.initials: CB
authors.lastname: Kasper
mesh_terms: D000818:Animals; D005075:Biological Evolution; D005415:Flavins / Q000737:chemistry* / Q000378:metabolism; D005420:Flavoproteins / Q000145:classification*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009251:NADPH-Ferrihemoprotein Reductase / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D009536:Niacinamide / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D051381:Rats
chemical_list: D005415:Flavins; D005420:Flavoproteins; D009536:Niacinamide; C008173:isoalloxazine; D009251:NADPH-Ferrihemoprotein Reductase