title: Aggregation of lasalocid A in membranes: a fluorescence study.
abstract: The aggregation behavior of the carboxylic ionophore, lasalocid A, has been studied in egg phosphatidylcholine vesicles by monitoring the intrinsic fluorescence of lasalocid A. Self quenching of lasalocid A fluorescence in vesicles of egg phosphatidylcholine suggests aggregation of lasalocid A. When aggregated lasalocid A is treated with increasing concentrations of lipid, there is an increase in fluorescence due to gradual reduction of self quenching on lateral dilution. This confirms the presence of loosely held non-covalent aggregates of lasalocid A in the membrane. This result is relevant in elucidating the molecular mechanism of cation transport by lasalocid A across membranes.
authors.affiliation: Centre for Cellular and Molecular Biology, Hyderabad, India.
authors.forename: A
authors.initials: A
authors.lastname: Chattopadhyay
authors.forename: S S
authors.initials: SS
authors.lastname: Komath
authors.forename: B
authors.initials: B
authors.lastname: Raman
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D007832:Lasalocid / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence
chemical_list: D008055:Lipids; D007832:Lasalocid