Purification and characterization of chicken follicle-stimulating hormone.
1. Highly purified chicken follicle-stimulating hormone (cFSH) was isolated from chicken pituitaries by differential extraction, sequential chromatography on HPLC cation and anion exchange columns, and gel filtration chromatography. 2. Purified cFSH (USDA-cFSH-K-1) had a potency of 77.44 units/mg in a chicken testes radioreceptor assay, and was biologically active in stimulating the secretion of progesterone by chicken granulosa cells. 3. Purified cFSH contained negligible luteinizing hormone and thyroid stimulating hormone activity. 4. The apparent molecular weight of cFSH was 38,000 Da and a single band on isoelectric focusing had a pI of 4.65.
Krishnan
K A
KA
USDA-ARS, Avian Physiology Laboratory, Beltsville Agricultural Research Center, MD 20705.
Proudman
J A
JA
Bahr
J M
JM
D000818:Animals; D001667:Binding, Competitive; D002645:Chickens; D005260:Female; D005640:Follicle Stimulating Hormone / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D007526:Isoelectric Point; D008970:Molecular Weight; D010903:Pituitary Gland, Anterior / Q000737:chemistry; D011374:Progesterone / Q000096:biosynthesis; D011962:Receptors, FSH / Q000378:metabolism
D011962:Receptors, FSH; D011374:Progesterone; D005640:Follicle Stimulating Hormone