Uptake and metabolism of a fluorescent sulfatide analogue in cultured skin fibroblasts.
The sulfatide fluorescent analogue N-lissamine rhodaminyl-(12-aminododecanoyl) cerebroside 3-sulfate was administered in the form of albumin complex to normal human skin fibroblasts and its metabolic fate was investigated. Ceramide, galactosylceramide, glucosylceramide, sphingomyelin and free acid, all containing the fluorophore lissamine rhodamine, have been synthesized as reference standards for the identification of the metabolic products. Ceramide appeared to be the main metabolic product present both in cell extract and medium, followed by galactosylceramide and sphingomyelin. Fluorescence microscopy of cells showed a marked perinuclear fluorescence.
Monti
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E
Department of Biomedical Sciences and Biotechnology, University of Brescia, Italy.
Preti
A
A
Novati
A
A
Aleo
M F
MF
Clemente
M L
ML
Marchesini
S
S
D000293:Adolescent; D002478:Cells, Cultured; D002675:Child, Preschool; D002855:Chromatography, Thin Layer; D005347:Fibroblasts / Q000378:metabolism; D005456:Fluorescent Dyes; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence; D012235:Rhodamines; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D013433:Sulfoglycosphingolipids / Q000378:metabolism*
D005456:Fluorescent Dyes; D012235:Rhodamines; D013433:Sulfoglycosphingolipids