title: Bacillus anthracis but not always anthrax.
abstract: Gram-positive bacilli isolated during epidemiological investigations which, on the basis of conventional tests, resemble Bacillus anthracis but which fail to produce the capsule or to induce anthrax in test animals have long been dismissed in clinical and veterinary laboratories as B. cereus or simply as unidentified Bacillus spp. and thereupon discarded as inconsequential. In this study, the application of newly available DNA probe, polymerase chain reaction and specific toxin antigen detection technology has revealed that a proportion of such strains are B. anthracis which lack the plasmid carrying the capsule gene (pXO2). While these techniques cannot, of course, be used to confirm the identities of strains resembling B. anthracis but which also lack the plasmid carrying the toxin genes (pXO1), the likelihood that these also are bona fide B. anthracis becomes more acceptable. (As yet no naturally occurring pXO1-/2+ strains have been found.) At this point, the significance of the presence of such avirulent forms of B. anthracis in specimens can only be a subject for speculation, but the possibility that they may be indicators of virulent parents somewhere in the system being examined must be considered.
authors.affiliation: Division of Biologics, Public Health Laboratory Service Centre for Applied Microbiology & Research, Porton Down, Salisbury, Wiltshire, UK.
authors.forename: P C
authors.initials: PC
authors.lastname: Turnbull
authors.forename: R A
authors.initials: RA
authors.lastname: Hutson
authors.forename: M J
authors.initials: MJ
authors.lastname: Ward
authors.forename: M N
authors.initials: MN
authors.lastname: Jones
authors.forename: C P
authors.initials: CP
authors.lastname: Quinn
authors.forename: N J
authors.initials: NJ
authors.lastname: Finnie
authors.forename: C J
authors.initials: CJ
authors.lastname: Duggleby
authors.forename: J M
authors.initials: JM
authors.lastname: Kramer
authors.forename: J
authors.initials: J
authors.lastname: Melling
mesh_terms: D000818:Animals; D000881:Anthrax / Q000382:microbiology*; D001408:Bacillus anthracis / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014774:Virulence
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial