The chicken IL-1 receptor: differential evolution of the cytoplasmic and extracellular domains.
The evolutionary conservation of a sequence or part of it can help to identify the essential functional and structural domains within a protein. We have cloned and characterised a cDNA coding for the type-I interleukin-1 receptor (IL-1R) of chick (ch) embryo fibroblasts. The comparison of the amino acid (aa) sequences of the avian with that of murine (m) and human (h) IL-1Rs shows a 60% homology. The intracellular domain is the most conserved region of the chIL-1R, showing 76-79% homology to the murine and human sequences, respectively. The striking conservation of the cytoplasmic region of the receptor is confirmed by its homology with the Toll receptor protein of Drosophila melanogaster. The alignment between the chicken and D. melanogaster proteins shows the presence of four aa blocks with more than 80% homology. The possible functional significance of this homology is discussed. The extracellular binding region of the receptor has a clearly recognisable immunoglobulin-like structure although the sequence divergence is higher than in the cytoplasmic domain.
Guida
S
S
Laboratory of Molecular Biology, Sclavo Research Center, Siena, Italy.
Heguy
A
A
Melli
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D029721:Drosophila Proteins; D007301:Insect Hormones / Q000737:chemistry; D007375:Interleukin-1; D008562:Membrane Glycoproteins / Q000737:chemistry; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry / Q000235:genetics*; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000737:chemistry / Q000235:genetics*; D017472:Receptors, Interleukin-1; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D051193:Toll-Like Receptors
D029721:Drosophila Proteins; D007301:Insect Hormones; D007375:Interleukin-1; D008562:Membrane Glycoproteins; D011948:Receptors, Antigen, T-Cell; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; C068721:Tl protein, Drosophila; D051193:Toll-Like Receptors