Cell-to-cell contact between normal fibroblasts and lymphoblasts deficient in lysosomal enzymes.
Human lymphoblasts deficient in iduronate sulfatase or in alpha-N-acetylglucosaminidase acquire discrete levels of enzyme activity after co-culture with human normal skin fibroblasts. This occurs by direct cell-to-cell contact and not by uptake of secreted fibroblast enzyme. The process is dependent on time and on the number of fibroblasts used. Electron-microscopic examination of the co-culture of the two cell types reveals extensive region of intimate contact. Fibroblastic projections appear frequently in close apposition with lymphoblast invaginations; a diffuse micropinocytotic activity is evident only in fibroblastic cells.
Di Natale
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P
Dipartimento di Biochimica e Biotecnologie Mediche, II Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli Federico II, Italy.
Annella
T
T
Daniele
A
A
Negri
R
R
Nitsch
L
L
D000118:Acetylglucosaminidase / Q000172:deficiency / Q000378:metabolism*; D002450:Cell Communication; D002472:Cell Transformation, Viral; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology; D006801:Humans; D007066:Iduronate Sulfatase / Q000378:metabolism*; D008214:Lymphocytes / Q000166:cytology* / Q000201:enzymology; D008854:Microscopy, Electron; D009083:Mucopolysaccharidoses / Q000201:enzymology; D016532:Mucopolysaccharidosis II
D007066:Iduronate Sulfatase; D000118:Acetylglucosaminidase