Simultaneous patient-side measurement of hemoglobin, glucose, and cholesterol in finger-stick blood.
We describe a multianalyte assay system for patient-side use comprising single-use plastic cartridges and a small monitor. Hemoglobin, glucose, and cholesterol can be simultaneously measured in 3 min in an unmeasured volume of blood. The sample is drawn by capillary action into four channels for delivery to assay-specific stacks containing a set of closely apposed layers. The distal layer is a membrane that acts as the optical surface for reflectance optics. For glucose and cholesterol assays, erythrocytes are removed by a fibrous filter layer and oxidase-peroxidase chemical reactions contained in the optical membrane generate a colored product. For hemoglobin measurement, blood is lysed by detergent contained in a porous disk. The amount of color reaching the optical membrane is measured by fiber optics. To ensure fail-safe operation, sensors verify sample sufficiency and degree of hemolysis. The assays perform comparably with laboratory methods.
Sterling
B
B
Biotrack, Ciba Corning Diagnostics, Mountain View, CA 94043.
Kiang
T
T
Subramanian
K
K
Saltman
M
M
Smart
W
W
Tsay
M
M
Sugarman
J
J
Patel
D
D
Monger
D
D
Martin
D
D
D001786:Blood Glucose / Q000032:analysis*; D002784:Cholesterol / Q000097:blood*; D003902:Detergents; D004912:Erythrocytes / Q000737:chemistry; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D010088:Oxidoreductases / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D010783:Photometry / Q000379:methods
D001786:Blood Glucose; D003902:Detergents; D006454:Hemoglobins; D002784:Cholesterol; D010088:Oxidoreductases; D010544:Peroxidases