Average density and size of microclusters of epidermal growth factor receptors on A431 cells.
For some hormone receptors, the early events of signal transduction depend on their molecular arrangement and interactions at the cell surface. An understanding of the mechanism of signal transduction in general needs a careful analysis of the receptor distribution. Here, we present the first quantitative measurement of epidermal growth factor receptor distribution on A431 cells obtained by scanning fluorescence correlation spectroscopy. Prior to epidermal growth factor binding, the A431 cell membrane presents an average surface density of 7.7-8.4 microclusters/microns 2, each containing an average of 130 receptors.
St-Pierre
P R
PR
Department of Chemistry, University of Western Ontario, London, Canada.
Petersen
N O
NO
D000911:Antibodies, Monoclonal; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D066246:ErbB Receptors / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence
D000911:Antibodies, Monoclonal; D066246:ErbB Receptors