title: Blood culture bottles are superior to lysis-centrifugation tubes for bacteriological diagnosis of spontaneous bacterial peritonitis.
abstract: The conventional method of ascitic fluid culturing was compared with the bedside inoculation of ascites into blood culture bottles and into lysis-centrifugation tubes. The conventional culture method was compared with the blood culture bottle method in 31 episodes of spontaneous bacterial peritonitis (SBP). Cultures were positive with the conventional culture method in 11 (35%) episodes and with the blood culture bottle method in 26 (84%) episodes (P less than 0.001). The lysis-centrifugation tube method was compared with the blood culture bottle method in 24 episodes of SBP. Cultures were positive with the lysis-centrifugation tube method in 11 (46%) episodes and with the blood culture bottle method in 19 (79%) episodes (P less than 0.05). Moreover, the blood culture bottle method also shortened the time needed for the detection of bacterial growth. In conclusion, bedside inoculation of ascites into blood culture bottles should be used routinely for patients with suspected SBP. Culturing of ascites in lysis-centrifugation tubes is more laborious than and inferior to that in blood culture bottles.
authors.affiliation: Department of Internal Medicine, University Hospital, Rotterdam, The Netherlands.
authors.forename: P D
authors.initials: PD
authors.lastname: Siersema
authors.forename: S
authors.initials: S
authors.lastname: de Marie
authors.forename: J H
authors.initials: JH
authors.lastname: van Zeijl
authors.forename: D J
authors.initials: DJ
authors.lastname: Bac
authors.forename: J H
authors.initials: JH
authors.lastname: Wilson
mesh_terms: D001201:Ascites / Q000150:complications / Q000382:microbiology; D001424:Bacterial Infections / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006095:Gram-Positive Cocci / Q000302:isolation & purification; D006801:Humans; D008103:Liver Cirrhosis / Q000150:complications; D010538:Peritonitis / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology