Incorporation of 2-fluorohistidine in murine protein in vivo.
The tissue distribution and time course of incorporation into acid insoluble (bound) and acid soluble (free) fractions of [3H]2-fluorohistidine is compared to that of U[14C]Histidine in mouse tissues in vivo. The cycloheximide-sensitive incorporation of 2-FHis is between 9 and 17 percent of that of His. Unlike [14C]His a major fraction, approximately 90% at 72 hrs, of isotope derived from [3H]2-FHis remains in tissues for a prolonged period in an acid soluble form. The excretion of isotope derived from [14C]His (T1/2 = 5 hr) is more rapid than from [3H]2-FHis (T1/2 = 11.4 hrs). 2-FHis, at doses from 100 to 250 mg/kg produce a reversible inhibition of growth in mice.
Creveling
C R
CR
Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Padgett
W L
WL
McNeal
E T
ET
Cohen
L A
LA
Kirk
K L
KL
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006639:Histidine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics; D007668:Kidney / Q000378:metabolism; D007930:Leucine / Q000378:metabolism / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011506:Proteins / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012995:Solubility; D014018:Tissue Distribution; D014316:Tritium
D002250:Carbon Radioisotopes; D011506:Proteins; D014316:Tritium; D006639:Histidine; C007749:2-fluorohistidine; D003513:Cycloheximide; D007930:Leucine