title: Bst71I: an isoschizomer of the type-IIS restriction enzyme, BbvI, recognizing the GCAGC(8/12) site.
abstract: A new type-IIS restriction enzyme, Bst71I, with the specificity 5'-GCAGC(N)8/3'-CGTCG(N)12 was isolated from Bacillus stearothermophilus (Promega No. 71). This enzyme is an isoschizomer of BbvI with somewhat improved characteristics for use by molecular biologists.
authors.affiliation: Promega Corporation, Madison, WI 53711.
authors.forename: T
authors.initials: T
authors.lastname: Schoenfeld
authors.forename: M
authors.initials: M
authors.lastname: Fiandt
authors.forename: M
authors.initials: M
authors.lastname: Schink
mesh_terms: D004247:DNA / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C025629:GCAGC-specific type II deoxyribonucleases