Regional and generalized changes in cytosolic free calcium in monocytes during phagocytosis.
We measured and visualized cytosolic free calcium ([Ca2+]i) in individual human peripheral blood monocytes during phagocytosis by using the fluorescent indicator fura-2. Monocytes exhibit a rapid rise in [Ca2+]i from a basal level of 75 +/- 11 nM to a peak level of 676 +/- 78 nM (means +/- standard errors of the means; P less than 0.001) by 34 +/- 5 s after contact with opsonized zymosan particles, and a thin rim of high [Ca2+]i was observed surrounding the ingested particle.
Kim
E
E
Department of Medicine, University of Virginia School of Medicine, Charlottesville 22908.
Enelow
R I
RI
Sullivan
G W
GW
Mandell
G L
GL
D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D010587:Phagocytosis
D002118:Calcium