Isolation and genetic complementation of a sulfolipid-deficient mutant of Rhodobacter sphaeroides.
All photosynthetic organisms are thought to contain the sulfolipid 6-sulfo-alpha-D-quinovosyl diacylglycerol. However, the pathway of sulfolipid biosynthesis has not been elucidated, and the functional or structural significance of this lipid is not known. Mutants of Rhodobacter sphaeroides deficient in sulfolipid accumulation were isolated by directly screening for altered sulfolipid content. The mutants had no apparent phenotype except for the sulfolipid deficiency. A gene, designated sqdA, which complemented one of the mutations was isolated and characterized. The putative sqdA gene product is a protein with a molecular mass of 33.6 kDa that has no sequence similarity to any enzyme of known function.
Benning
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MSU-DOE-Plant Research Laboratory, Michigan State University, East Lansing 48824-1312.
Somerville
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002849:Chromatography, Gas; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012242:Rhodobacter sphaeroides / Q000737:chemistry / Q000235:genetics*; D013431:Sulfates / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008055:Lipids; D013431:Sulfates; C015518:sulfolipids; C073765:sqdA protein, Rhodobacter sphaeroides