title: Incomplete process of recombinant human platelet-derived growth factor produced in yeast and its effect on the biological activity.
abstract: Partially purified recombinant human Platelet-derived Growth Factor BB homodimer isolated from yeast culture media contains variable amounts of unprocessed PDGF-BB. This unprocessed PDGF-BB is found as a result of incomplete cleavage of the precursor to form the mature protein. Although the signal peptide is efficiently removed, a fraction of the PDGF secreted has an extended sequence corresponding to the truncated yeast alpha-factor leader. The data suggest that it is the amino acid chain from the truncated a-factor leader and not the sugar moiety attached to it that is responsible for the higher mitogenic activity found in this unprocessed molecule compared to highly purified PDGF-BB.
authors.affiliation: Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608.
authors.forename: M
authors.initials: M
authors.lastname: Calderón-Cacia
authors.forename: P
authors.initials: P
authors.lastname: Tekamp-Olson
authors.forename: J
authors.initials: J
authors.lastname: Allen
authors.forename: C
authors.initials: C
authors.lastname: George-Nascimento
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D006031:Glycosylation; D006801:Humans; D046911:Macromolecular Substances; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010982:Platelet-Derived Growth Factor / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D021382:Protein Sorting Signals / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D046911:Macromolecular Substances; D008934:Mitogens; D010982:Platelet-Derived Growth Factor; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011994:Recombinant Proteins