Mammalian expression of the human sex steroid-binding protein of plasma (SBP or SHBG) and testis (ABP). Characterization of the recombinant protein.
A full-length 1,209 bp cDNA encoding the human sex steroid-binding protein of plasma (SBP or SHBG) and testis (ABP) was constructed and expressed in BHK-21 cells. The sequence agrees with the published gene and protein sequences. The cells were found to secrete SBP following transfection and G418r selection. The recombinant protein binds 5 alpha-dihydrotestosterone with a Kd of 0.28 nM. It also binds testosterone and 17 beta-estradiol but not progesterone, estrone or cortisol revealing a steroid-binding specificity identical to that of human SBP. SDS-PAGE patterns are less complex than human SBP and show a monomeric molecular weight of about 43 kDa.
Hagen
F S
FS
Zymogenetics Inc., Seattle, WA 98105.
Arguelles
C
C
Sui
L M
LM
Zhang
W
W
Seidel
P R
PR
Conroy
S C
SC
Petra
P H
PH
D001483:Base Sequence; D001769:Blood; D003001:Cloning, Molecular; D004247:DNA; D015870:Gene Expression; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009928:Organ Specificity / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012738:Sex Hormone-Binding Globulin / Q000235:genetics* / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
D011994:Recombinant Proteins; D012738:Sex Hormone-Binding Globulin; D004247:DNA