Purification, characterization and function of bacterioferritin from the cyanobacterium Synechocystis P.C.C. 6803.
Storage and buffering of iron is achieved by a class of proteins, the ferritins, widely distributed throughout the living kingdoms. All ferritins have in common their three-dimensional structure and their ability to store large amounts of iron in their central cavity. However, eukaryotic ferritins from plants and animals and bacterioferritins have no sequence similarity, and besides non-haem iron bacterioferritins contain haem residues whereas eukaryotic ferritins do not. In this paper we report the first purification and characterization of a bacterioferritin from a cyanobacterium. It has a molecular mass of 400 kDa and is built up from 19 kDa subunits. Its N-terminal sequence shows 73% identity with that of the Escherichia coli bacterioferritin subunit. It contains 2300 atoms of iron and 1500 molecules of phosphate per ferritin molecule and 0.25 haem residue per subunit; the alpha-peak of the cytochrome has its maximum at 559 nm. In contrast with what is known for eukaryotic ferritins, we found that bacterioferritin from Synechocystis is not inducible by iron under the conditions that we have tested and that it has a constant concentration whatever the iron status of the cells, even at very low iron concentration. Bacterioferritin from Synechocystis P.C.C. 6803 is fully assembled in vivo and it is shown by labelling with 59Fe that it is able to load iron in vitro as well as in vivo. Bacterioferritin from Synechocystis is shown to have an iron-buffering function while the bulk of cellular iron is found associated with a pool of low-molecular-mass electronegative molecules. The role of Synechocystis bacterioferritin in iron metabolism is discussed.
Laulhère
J P
JP
Laboratoire de Biologie Moléculaire Végétale, Centre National de la Recherche Scientifique (Unité de Recherche Associée n. 1178), Grenoble, France.
Labouré
A M
AM
Van Wuytswinkel
O
O
Gagnon
J
J
Briat
J F
JF
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D000458:Cyanobacteria / Q000737:chemistry*; D003573:Cytochrome b Group / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry; D005293:Ferritins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006418:Heme / Q000032:analysis; D007501:Iron / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010710:Phosphates / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D013053:Spectrophotometry
D001426:Bacterial Proteins; D003573:Cytochrome b Group; D010710:Phosphates; D006418:Heme; D005293:Ferritins; C018032:bacterioferritin; D007501:Iron