Estrogen inhibits calbindin-D28k expression in mouse uterus.
The cellular localization and hormonal controls of calbindin-D9k expression in the rodent reproductive tract have suggested new functions for this protein. The present studies were undertaken to extend the earlier studies of calbindin-D9k to the related protein, calbindin-D28k. Immunohistochemical studies revealed that calbindin-D28k was absent from female rat reproductive tissues, but was abundantly expressed in immature mouse uterus and oviduct. Immunoreactivity was restricted to the endometrial and glandular epithelium of the uterus and the oviductal epithelium. Neither 1,25-dihydroxyvitamin D- nor strontium-containing diets (to blunt 1,25-dihydroxyvitamin D production) affected expression of calbindin-D28k. Uterine, but not oviductal, calbindin-D28k decreased markedly at sexual maturity; this pattern persisted in pregnant mice and was reproduced in immature mice by the administration of estradiol (3 micrograms/day for 3 days). RNA extraction and Northern analyses demonstrated that estrogen markedly decreased calbindin-D28k mRNA abundance in the uterus, but not in the oviduct. These findings suggest that estrogen affects mammalian calbindin-D28k expression and represent a rare example of estrogen-induced down-regulation of gene expression.
Opperman
L A
LA
Department of Pathology, University of Virginia School of Medicine, Charlottesville 22908.
Saunders
T J
TJ
Bruns
D E
DE
Boyd
J C
JC
Mills
S E
SE
Bruns
M E
ME
D000818:Animals; D015152:Blotting, Northern; D064092:Calbindin 1; D064026:Calbindins; D002117:Calcitriol / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D004717:Endometrium / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D007150:Immunohistochemistry; D051379:Mice; D010057:Oviducts / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D014599:Uterus / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism
C576499:Calb1 protein, mouse; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D004967:Estrogens; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G; C576507:S100g protein, mouse; C576508:S100g protein, rat; D004958:Estradiol; D002117:Calcitriol