Protein kinase C activation in murine endothelioma cell lines containing middle T antigen stimulated by platelet-activating factor.
Murine endothelial cell lines containing middle T antigen provide a good model to study the biology of capillary cells of different anatomical districts. These cell lines obtained from skin (sEnd.1), brain (bEnd.4), thymus (tEnd-1) and embryo tissue (eEnd.1) are activated by platelet-activating factor (PAF). PAF, but not lyso-PAF or the enantiomer (S)-PAF, induces the rapid translocation of protein kinase C (PKC) from the cytosol to the membrane. Maximal translocation of the enzyme is achieved after 5 min in all cell lines tested. The ED50 of PAF able to promote PKC translocation has a value of 5 nM in sEnd.1, bEnd.4 and eEnd.1, and 20 nM in tEnd.1, suggesting a different sensitivity between these cell lines. The data indicate that PAF activates capillary cells, thus explaining its activation of microvascular beds.
Bussolino
F
F
Dipartimento di Genetica, Biologia e Chimica Medica, Universita di Torino, Italy.
Guglielmetti
A
A
Ghigo
D
D
Pescarmona
G
G
Bosia
A
A
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D001932:Brain Neoplasms / Q000201:enzymology; D004789:Enzyme Activation; D006390:Hemangioendothelioma / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D012878:Skin Neoplasms / Q000201:enzymology; D013237:Stereoisomerism; D013950:Thymus Gland / Q000201:enzymology; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000952:Antigens, Polyomavirus Transforming; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D011493:Protein Kinase C