Binding of dietary cobalt to sarcoplasmic reticulum proteins.
It has previously been shown that cobalt accumulates in the myocardium of rats, mainly the sarcoplasmic reticulum (SR) and the mitochondrial inner membrane. In order to investigate the mode of accumulation of cobalt in the SR, rats were given a dietary cobalt supplementation of 40 mg of CoSO4 x 7H2O kg-1 body wt, after which the rats were sacrificed and the sarcoplasmic reticulum was isolated. The SR proteins were subjected to analysis by polyacrylamide gel electrophoresis followed by protein staining and determination of the content of cobalt in each protein band. The major cobalt-binding protein was found to have a molecular weight of about 100,000; a 200,000 molecular weight protein was also found to bind cobalt, although less extensively. These results suggest that cobalt is bound to the monomeric and dimeric forms of Ca(2+)-ATPase in the SR of the myocardium.
Persson
B
B
Department of Biochemistry, University of Stockholm, Sweden.
Carlenor
E
E
Clyne
N
N
Hultman
E
E
Lins
L E
LE
Pehrsson
S K
SK
Rydström
J
J
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D003035:Cobalt / Q000378:metabolism*; D004032:Diet; D009206:Myocardium / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D011506:Proteins; D003035:Cobalt; D000252:Calcium-Transporting ATPases