title: Oxidation of residue 45 mutant forms of pig deoxymyoglobin with [Fe(CN)6]3-.
abstract: The effect of replacement of the highly conserved Lys45 residue in pig myoglobin (Mb) with His, Ser, Glu, and Arg has been investigated. Rate constants/M-1 s-1 at 25 degrees C and pH 8.0, I = 0.100 M (NaCl), for the oxidation of deoxyMb with [Fe(CN)6]3- have been determined, and are for wild-type Lys45 (2.83 x 10(6)), His45 (1.02 x 10(6)), Ser45 (1.12 x 10(6)), Glu45 (0.87 x 10(6)), and Arg45 (3.06 x 10(6)). It is concluded that charge on the residue at position 45 has only a mild effect on reactivity, and that this is unlikely to be the site for electron transfer.
authors.affiliation: Department of Chemistry, The University, Newcastle upon Tyne, U.K.
authors.forename: B J
authors.initials: BJ
authors.lastname: Zhang
authors.forename: S J
authors.initials: SJ
authors.lastname: Smerdon
authors.forename: A J
authors.initials: AJ
authors.lastname: Wilkinson
authors.forename: A G
authors.initials: AG
authors.lastname: Sykes
mesh_terms: D000818:Animals; D004563:Electrochemistry; D005292:Ferricyanides / Q000378:metabolism*; D007700:Kinetics; D008239:Lysine / Q000378:metabolism; D015394:Molecular Structure; D016296:Mutagenesis; D009211:Myoglobin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship; D013552:Swine
chemical_list: D005292:Ferricyanides; D009211:Myoglobin; C055547:deoxymyoglobin; D008239:Lysine; C028033:potassium ferricyanide