title: Charge effect in the interaction of doxorubicin and derivatives with polydeoxynucleotides.
abstract: The equilibrium interaction of doxorubicin and its N-acetyl derivative with a series of purine-pyrimidine alternating polydeoxynucleotides has been studied through spectrofluorometry to assess the relevance of the electrostatic contribution to DNA intercalation. The results have shown that: (a) the suppression of the positive charge on the aminosugar has: (I) a profound negative effect on the free energy of intercalation, as expected, and (II) a negligible influence on the base specificity, which supports the notion of an essentially electrostatic effect of N-acetylation on intercalation; (b) a reasonably good accord with the demands of a polyelectrolytic model, due to Friedman and Manning, is found.
authors.affiliation: Physics Department, Institute of Biosciences, UNESP, São José do Rio Preto, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Ruggiero
authors.forename: L E
authors.initials: LE
authors.lastname: Xodo
authors.forename: A
authors.initials: A
authors.lastname: Ciana
authors.forename: G
authors.initials: G
authors.lastname: Manzini
authors.forename: F
authors.initials: F
authors.lastname: Quadrifoglio
mesh_terms: D004247:DNA / Q000378:metabolism*; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000378:metabolism*; D004560:Electricity; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D004317:Doxorubicin; D004247:DNA