The xylanase introns from Cryptococcus albidus are accurately spliced in transgenic tobacco plants.
The xylanase gene from Cryptococcus albidus contains seven introns. Genomic and cDNA clones under the control of the CaMV 35S promoter were transferred into tobacco plants using Agrobacterium-mediated cell transformation. The genes were transcribed and the mRNAs were amplified by the polymerase chain reaction using primers on each side of the intron region. About 90% of the amplification products from plants transformed with the genomic clone corresponded to the size of the pre-mRNA (1.2 kb) and 10% represented the spliced product (0.85 kb). The 0.85 kb fragment was cloned and sequenced and the result indicated that the introns from the xylanase gene were accurately spliced by the plant cells.
Laliberté
J F
JF
Institut Armand-Frappier, Ville de Laval, Québec, Canada.
Nicolas
O
O
Durand
S
S
Morosoli
R
R
D001483:Base Sequence; D003454:Cryptococcus / Q000201:enzymology / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics*; D043325:Xylan Endo-1,3-beta-Xylosidase
D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D012322:RNA Precursors; D006026:Glycoside Hydrolases; D043325:Xylan Endo-1,3-beta-Xylosidase