An enhanced chemiluminescence detection system combined with a modified immunoblot technique for the detection of low molecular weight IgM in sera from healthy adults and neonates.
An enhanced chemiluminescence detection system combined with a modified immunoblot technique is described for the detection of low molecular weight IgM (LMW IgM) in human sera and cell culture supernatants at levels down to 2 pg/ml. This detection system is reliable, specific and more sensitive than the previously described chromogenic detection system. Importantly, this method has, for the first time, revealed LMW IgM in the sera from all 34 healthy adults and 26 neonatal cord bloods tested. A significant linear correlation was observed between the LMW IgM and the total circulating IgM in both the healthy adult sera (r = 0.88, p less than 0.001) and cord blood sera (r = 0.98, p less than 0.001).
Xu
H J
HJ
Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, South Australia.
Umapathysivam
K
K
McNeilage
J
J
Gordon
T P
TP
Roberts-Thomson
P J
PJ
D000328:Adult; D000937:Antigen-Antibody Reactions; D002478:Cells, Cultured; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007075:Immunoglobulin M / Q000032:analysis* / Q000737:chemistry; D007231:Infant, Newborn; D008163:Luminescent Measurements; D008970:Molecular Weight
D007075:Immunoglobulin M