Pseudomonas aeruginosa acid phosphatase. Activation by divalent cations and inhibition by aluminium ion.
In Pseudomonas aeruginosa, the effect of different cations on the acid phosphatase activity was studied in order to acquire more information related to a previously proposed mechanism, involving the coordinated action of this enzyme with phospholipase C. Although the natural substrate of this enzyme is phosphorylcholine, in order to avoid the possible interaction of its positive charge and those of the different cations with the enzyme molecule, the artificial substrate p-nitrophenylphosphate was utilized. Kinetic studies of the activation of acid phosphatase (phosphorylcholine phosphatase) mediated by divalent cations Mg2+, Zn2+ and Cu2+ revealed that all these ions bind to the enzyme in a compulsory order (ordered bireactant system). The Km values obtained for p-NPP in the presence of Mg2+, Zn2+ and Cu2+ were 1.4 mM, 1.0 mM and 3.5 mM, respectively. The KA values for the same ions were 1.25 mM, 0.05 mM and 0.03 mM, respectively. The Vmax obtained in the presence of Cu2+ was about twofold higher than that obtained in the presence of Mg2+ or Zn2+. The inhibition observed with Al3+ seems to be a multi-site inhibition. The K'app and n values, from the Hill plot, were about 0.25 mM and 4.0 mM, respectively, which were independent of the metal ion utilized as activator. It is proposed that the acid phosphatase may exert its action under physiological conditions, depending on the availability of either one of these metal ions.
Domenech
C E
CE
Departamento de Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Lisa
T A
TA
Salvano
M A
MA
Garrido
M N
MN
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000535:Aluminum / Q000494:pharmacology*; D002413:Cations, Divalent; D003300:Copper / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D015032:Zinc / Q000494:pharmacology
D002413:Cations, Divalent; D003300:Copper; D000535:Aluminum; D000135:Acid Phosphatase; D008274:Magnesium; D015032:Zinc