Homeoviscous adaptation under pressure: the pressure dependence of membrane order in brain myelin membranes of deep-sea fish.
Steady-state and time-resolved anistropy of 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence have been used to compare the hydrocarbon order of brain myelin membranes from a shallow water (plaice) and two deep-sea fish species (Coryphenoides rupestris and Coryphenoides armatus). At atmospheric pressure the deep sea fish displayed lower steady-state anisotropies than shallow water species although the pressure dependence of anisotropy was similar in all species. Time-resolved measurements allowed the separate determination of the rate of probe motion from the amplitude of that motion. Anisotropy decays were analysed in terms of two correlation times and a constant (r infinity). The r infinity and mean value of P2 order parameter for all species increased with pressure, the graphs for deep-sea species being translated to higher pressures relative to shallow-water species. The resulting pressure coefficients for C. armatus was distinctly less than for the two shallower species. These time-resolved studies show that the interspecific differences provide for similar order parameters in all three species when corrected to their respective habitat conditions of pressure and temperature. This indicates that myelin order is highly conserved despite the profound ordering effects of high hydrostatic pressure.
Behan
M K
MK
Department of Environmental and Evolutionary Biology, University of Liverpool, UK.
Macdonald
A G
AG
Jones
G R
GR
Cossins
A R
AR
D000222:Adaptation, Physiological; D000818:Animals; D001921:Brain / Q000502:physiology*; D001923:Brain Chemistry; D002462:Cell Membrane / Q000737:chemistry* / Q000502:physiology; D005399:Fishes; D005454:Fluorescence Polarization; D006874:Hydrostatic Pressure; D009186:Myelin Sheath / Q000737:chemistry* / Q000502:physiology; D014783:Viscosity