title: Analysis of a novel linkage unit of O-linked carbohydrates from the crystalline surface layer glycoprotein of Clostridium thermohydrosulfuricum S102-70.
abstract: The surface layer glycoprotein of Clostridium thermohydrosulfuricum S102-70 was shown to contain a new type of glycan chain. Different from all known eubacterial glycoproteins, the saccharide moiety consists only of six sugar residues without any repeat sequences. Proteolytic digestion of purified S-layer glycoprotein resulted in isolation of several glycopeptide fractions. These are composed of the same hexasaccharide portion but are linked to oligopeptides of different length. One of them contains only a single amino acid. As concluded from chemical analyses and proton and carbon nuclear magnetic resonance spectroscopy of this preparation, the hexasaccharide moiety is linked via a novel O-glycosidic linkage. This is a beta-D-glucose residue linked to the phenolic hydroxyl group of tyrosine in intact S-layer glycoprotein.
authors.affiliation: Zentrum für Ultrastrukturforschung, Universität für Bodenkultur, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Messner
authors.forename: R
authors.initials: R
authors.lastname: Christian
authors.forename: J
authors.initials: J
authors.lastname: Kolbe
authors.forename: G
authors.initials: G
authors.lastname: Schulz
authors.forename: U B
authors.initials: UB
authors.lastname: Sleytr
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003013:Clostridium / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data
chemical_list: D001425:Bacterial Outer Membrane Proteins; D006020:Glycopeptides; D006023:Glycoproteins