Reduced expression of neutrophil CD11b and CD16 after severe traumatic injury.
Overwhelming sepsis continues to be a major source of morbidity and mortality in patients who have sustained severe traumatic injury. Recently, much interest has been focused on the role of the peripheral blood neutrophil (PMN) in infections in these patients. Two surface receptors, CD11b (CR3) and CD16 (Fc gamma RIII), are thought to participate in bacterial phagocytosis and are both present on greater than 85% of normal PMNs. We have previously shown that cells that lack both of these receptors have markedly reduced phagocytic function. The purpose of this study was to determine the effect of severe trauma on the expression of these PMN receptors. Twenty severe trauma patients, age 19-70 years, presenting with an initial APACHE II score of greater than or equal to 10 were arbitrarily divided into two groups to define severity of injury: Group A, initial APACHE II of 10-18 (n = 11) and Group B, initial APACHE II of 19-25 (n = 9). Blood was obtained on admission, on Day 3, and weekly thereafter. PMNs were stained with fluorochrome-labeled monoclonal antibodies directed against CD11b and CD16 and then analyzed by flow cytometry. Controls consisted of 14 normal adults, age 20-65 years. The percentage and absolute numbers of CD11b+/CD16+ PMNs were determined for each patient or control sample. ANOVA and multiple comparison of variables (P = 0.05) were performed for each week. Values for Group A were different from controls at Weeks 0, 1, and 3. Values for Group B were significantly lower than those of controls at all weeks.(ABSTRACT TRUNCATED AT 250 WORDS)
White-Owen
C
C
Department of Surgery, University of Cincinnati, Ohio.
Alexander
J W
JW
Babcock
G F
GF
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D016177:Macrophage-1 Antigen / Q000378:metabolism*; D009504:Neutrophils / Q000276:immunology*; D011961:Receptors, Fc / Q000378:metabolism*; D017452:Receptors, IgG; D013997:Time Factors; D014947:Wounds and Injuries / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D016177:Macrophage-1 Antigen; D011961:Receptors, Fc; D017452:Receptors, IgG