Electron immunocytochemical localization of Actinobacillus actinomycetemcomitans leukotoxin.
The Actinobacillus actinomycetemcomitans leukotoxin was localized in A. actinomycetemcomitans bacteria using an electron immunocytochemical thin-section labeling method. An immuno-dot blot procedure was initially used to ascertain the optimal specimen fixation. This consisted of a periodate-lysine-paraformaldehyde (2%) fixative in a phosphate buffer followed by embedding in LR White. Affinity-purified toxin was used to produce a monospecific polyclonal antibody. The reaction sites were visualized with a colloidal gold-tagged reporter antibody. The leukotoxin was found to be localized either in the cell envelope and/or in membranous vesicles on the outer surface of the bacterial cell. These results support previous observations indicating the same location.
Berthold
P
P
School of Dental Medicine, University of Pennsylvania.
Forti
D
D
Kieba
I R
IR
Rosenbloom
J
J
Taichman
N S
NS
Lally
E T
ET
D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry* / Q000648:ultrastructure; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001427:Bacterial Toxins / Q000032:analysis*; D005098:Exotoxins / Q000032:analysis*; D006046:Gold; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009055:Mouth / Q000382:microbiology
D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; C018579:leukotoxin; D006046:Gold