Comparison of the inducing effect of dehydroepiandrosterone on hepatic peroxisome proliferation-associated enzymes in several rodent species. A short-term administration study.
The in-vivo effect of dehydroepiandrosterone (DHEA) on hepatic enzyme activities of rats, mice, hamsters and guinea pigs was investigated. After DHEA treatment (300 mg/kg body weight, per os, 14 days), the activities of peroxisomal beta-oxidation, catalase, carnitine acetyltransferase, carnitine palmitoyltransferase, lauric acid omega-hydroxylation, 1-acylglycerophosphocholine acyltransferase, malic enzyme and cytosolic palmitoyl-CoA hydrolase were increased in rats and in mice although to a smaller extent in the latter. These enzyme activities, however, were unchanged in hamsters with the exception of omega-hydroxylation (2.5-fold increase) and 1-acylglycerophosphocholine acyltransferase (2.0-fold increase). No significant changes were observed in any of these enzyme activities in guinea pigs. Immunoblot analysis confirmed the induction of peroxisomal acyl-CoA oxidase and enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional enzyme in rats and mice. These results indicate that there are species differences in the inducing effect of DHEA on hepatic peroxisome proliferation-associated enzymes, which correlates well with the enzyme induction observed with other peroxisome proliferators.
Sakuma
M
M
Department of Clinical Biochemistry, Tokyo College of Pharmacy, Japan.
Yamada
J
J
Suga
T
T
D000818:Animals; D001835:Body Weight; D006224:Cricetinae; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D006168:Guinea Pigs; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008830:Microbodies / Q000201:enzymology; D009929:Organ Size; D010084:Oxidation-Reduction; D051381:Rats; D011916:Rats, Inbred F344
D003687:Dehydroepiandrosterone