A membrane vesicle/ribosome preparation from Serratia marcescens elicits peritoneal exudate cells expressing both tumoricidal and bactericidal activity.
A biological response modifier called ImuVert, derived from the bacterium Serratia marcescens, produced long-lasting elevation of peritoneal exudate cell (PEC) numbers after intraperitoneal injection into mice. These cells had enhanced ability to phagocytose both latex beads and opsonized Listeria monocytogenes. PEC harvested 2-14 days after a single injection of ImuVert killed L. monocytogenes, and ImuVert protected mice from infection by L. monocytogenes, measured both by LD50 and bacterial growth in vivo. Cells harvested 7 and 14 days after ImuVert injection also were tumoricidal, measured as killing of P815 mastocytoma cells, and ImuVert induced macrophages to express tumoricidal properties in vitro. These data suggest that ImuVert has a unique ability to induce a chronic inflammatory response, as other agents do not induce such a long-lasting influx of bactericidal inflammatory cells that also show tumoricidal activity. The consequences of this response appear to include protection from infection by certain bacteria.
McCall
C
C
Cell Technology Inc., Boulder, Colorado 80301.
Weimer
L
L
Baldwin
S
S
Riches
D W
DW
Canono
B
B
Campbell
P A
PA
D000818:Animals; D001688:Biological Products; D001770:Blood Bactericidal Activity / Q000187:drug effects*; D002452:Cell Count; D002462:Cell Membrane / Q000502:physiology; D011415:Complement Factor B / Q000032:analysis; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005122:Exudates and Transudates / Q000166:cytology*; D005260:Female; D007155:Immunologic Factors / Q000494:pharmacology*; D007700:Kinetics; D008089:Listeria monocytogenes / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D012270:Ribosomes / Q000737:chemistry*; D012706:Serratia marcescens / Q000648:ultrastructure*
D001688:Biological Products; D007155:Immunologic Factors; C062792:ImuVert; D011415:Complement Factor B