title: A delayed and sustained rise of cytosolic calcium is elicited by oxidized LDL in cultured bovine aortic endothelial cells.
abstract: Bovine aortic endothelial cells (BAEC) pulsed for 5 h with mildly oxidized low density lipoproteins (LDL), exhibited a broad, sustained and high peak of [Ca2+]i occurring several hours after the end of the pulse and reaching very high [Ca2+]i values (around 2500-3000 nmol/l) and a concomitant drop of cytosolic pH (around 0.2-0.3 pH units) without any loss of cell viability. When BAEC were continuously pulsed with oxidized LDL, the peak of [Ca2+]i was more sustained than in short pulse experiments and was associated with irreversible morphological changes usually associated with cytotoxic events (blebbing) and with a marked loss of viability. The potential involvement of these biochemical and morphological changes in atherogenesis are discussed.
authors.affiliation: Department of Biochemistry, Faculty of Medicine in Rangueil, University Paul Sabatier, Toulouse, France.
authors.forename: A
authors.initials: A
authors.lastname: Nègre-Salvayre
authors.forename: G
authors.initials: G
authors.lastname: Fitoussi
authors.forename: V
authors.initials: V
authors.lastname: Réaud
authors.forename: M T
authors.initials: MT
authors.lastname: Pieraggi
authors.forename: J C
authors.initials: JC
authors.lastname: Thiers
authors.forename: R
authors.initials: R
authors.lastname: Salvayre
mesh_terms: D000818:Animals; D001011:Aorta; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction
chemical_list: D008077:Lipoproteins, LDL; D002118:Calcium