Primary culture of collecting duct cell epithelium from neonate rabbit kidney in monolayer.
Cortical collecting duct cells from rabbit neonate kidney capsula fibrosa explants were grown in low (0.1%) serum medium on permeable culture supports. We found that over a period of 3-4 days in culture, the cells migrated from the explants, to form monolayers. The cells in monolayer displayed morphology similar to adult collecting duct cells. Approximately 24% of cells grown in monolayer were found to bind a specific beta-cell-probe, peanut agglutinin. Single cells were isolated by brief exposure to 0 Ca(2+)-Mg2+ solutions and the whole-cell clamp technique was used to measure macroscopic currents. The whole-cell conductance of 1.35 +/- 0.1 nS was reduced to 0.9 +/- 0.2 nS in the presence of 100 microM-amiloride. We conclude that these cells express cell membrane properties consistent with those of the collecting duct and that it may be a useful model for the study of cellular transport mechanisms and the factors controlling development of collecting duct cell types.
White
S
S
Department of Physiology, University of Leeds.
Reeve
H
H
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004594:Electrophysiology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000166:cytology* / Q000378:metabolism; D037102:Lectins; D008854:Microscopy, Electron; D019887:Peanut Agglutinin; D011817:Rabbits
D037102:Lectins; D019887:Peanut Agglutinin