title: Transient accumulation and subsequent rapid loss of messenger RNA encoding high molecular mass CD45 isoforms after T cell activation.
abstract: High molecular mass isoforms of CD45 protein tyrosine phosphatase, predominantly CD45RA, are expressed on naive T cells with increasing density during the first 2 days of cellular activation, and subsequently down-regulated concurrent with increasing expression of the low Mr isoform, CD45RO. We show this to be de novo synthesis of CD45RA dependent upon both RNA and protein synthesis. Using a probe shown to detect mRNA encoding the alternatively spliced abc, ab, bc, and b exon isoforms of CD45, the expression of CD45 was analyzed. Kinetic studies of the transition from high to low Mr CD45 mRNA indicate an immediate decrease in the level of high Mr CD45 mRNA after mitogen stimulation of T cells, quickly followed at 4 h by an increase to above steady state levels. This is consistent with the transitory increase in cell-surface density of CD45RA observed at 1 to 2 days poststimulation. Within 24 h of stimulation the level of high Mr CD45 mRNA declines precipitously such that little or no mRNA encoding any of the alternatively spliced exons is detectable. High levels of CD45 mRNA are detectable at later points but this does not hybridize with the Sfa N1 probe recognizing the abc exons suggesting that only the CD45RO mRNA splicing pattern is operative. We also show that CD45 mRNA have a relatively short t1/2. In mitogen-stimulated cells the t1/2 of high and low m.w. CD45 mRNA was estimated at 2.25 h and 3.5 h, respectively. In unstimulated T cells the t1/2 of high Mr CD45 mRNA was estimated at 2.8 h. CD45 mRNA is super-induced in the presence of the protein synthesis inhibitor, cycloheximide, indicating that the degradation and/or splicing of CD45 mRNA is controlled by a labile pathway, and suggesting that mechanisms may exist in vivo to prolong synthesis of CD45RA. The kinetics of accumulation for high Mr CD45 mRNA are very similar to those of the TCR beta-chain and pp56lck suggesting that these functionally linked signaling molecules are regulated in tandem. This implicates stringent molecular control mechanisms on the production of mRNA encoding either high or low m.w. isoforms, consistent with the fundamental role of CD45 in signal transduction, and the apparent need to selectively and sequentially express functionally distinct external CD45 domains.
authors.affiliation: Department of Immunology, University of Alberta, Edmonton, Canada.
authors.forename: J P
authors.initials: JP
authors.lastname: Deans
authors.forename: H M
authors.initials: HM
authors.lastname: Serra
authors.forename: J
authors.initials: J
authors.lastname: Shaw
authors.forename: Y J
authors.initials: YJ
authors.lastname: Shen
authors.forename: R M
authors.initials: RM
authors.lastname: Torres
authors.forename: L M
authors.initials: LM
authors.lastname: Pilarski
mesh_terms: D015703:Antigens, CD / Q000235:genetics*; D003513:Cycloheximide / Q000494:pharmacology; D015342:DNA Probes; D015870:Gene Expression / Q000187:drug effects; D006649:Histocompatibility Antigens / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D017493:Leukocyte Common Antigens; D008213:Lymphocyte Activation; D008970:Molecular Weight; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D015703:Antigens, CD; D015342:DNA Probes; D006649:Histocompatibility Antigens; D012333:RNA, Messenger; D003513:Cycloheximide; D017493:Leukocyte Common Antigens