Immunological characterization of protective antigens prepared by alkaline treatment of whole cells and from the culture filtrate of Erysipelothrix rhusiopathiae.
Culture filtrate and alkaline-extracted antigens from whole cells of an attenuated strain of Erysipelothrix rhusiopathiae (strain Koganei: serovar 1a) were fractionated with ammonium sulfate; both induced protective immunity in mice. Sephadex G-200 gel filtration revealed three protein fractions in the alkaline-extracted antigen and four protein fractions in the culture filtrate antigen. A fraction in the alkaline extract (NaOH P-2) and in the culture filtrate (CF P-2) induced protection in mice against challenge with a different serovar strain (strain Agata: serovar 5). Anti-NaOH P-2 and anti-CF P-2 mouse sera were protective against different serovars. Glycoprotein fraction derived from CF P-2 antigen by affinity chromatography with Con A-Sepharose 4B did not show protective activity. Western blotting between the antisera (anti-NaOH P-2, Anti-CF P-2 and anti-Koganei strain) and the antigens (NaOH P-2, and sonicated antigens of Agata, Fujisawa and Koganei strains) showed strong recognition of the same bands at 62, 42 and 41 kDa.
Kobayashi
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Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Sato
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Hirose
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Saito
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D000645:Ammonium Sulfate; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines; D015153:Blotting, Western; D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004888:Erysipelothrix / Q000276:immunology*; D004889:Erysipelothrix Infections / Q000517:prevention & control*; D007106:Immune Sera / Q000276:immunology; D007116:Immunization, Passive; D051379:Mice; D008970:Molecular Weight; D012972:Sodium Hydroxide; D013047:Specific Pathogen-Free Organisms
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007106:Immune Sera; D012972:Sodium Hydroxide; D000645:Ammonium Sulfate