Molecular cloning and characterization of catechol 2,3-dioxygenases from biphenyl/polychlorinated biphenyls-degrading bacteria.
Catechol 2,3-dioxygenases were cloned from Alcaligenes sp. KF711, Pseudomonas putida KF715, and Achromobacter xylosoxidans KF701 which are biphenyl/polychlorinated biphenyls-degrading bacteria. All of the cloned enzymes were purified by preparative polyacrylamide gel electrophoresis (PAGE). The purified catechol 2,3-dioxygenases were significantly different from one another in ring-fission activities to catechol and its derivatives. The catechol 2,3-dioxygenase from Alcaligenes sp. KF711 exhibited higher ring-fission activity to 4-chlorocatechol than those from P. putida KF715 and A. xylosoxidans KF701. In electrophoretic mobilities, the three enzymes were different from one another on nondenaturing PAGE but the same on SDS-PAGE.
Chang
H
H
College of Pharmacy, Chungbuk National University, Korea.
Lee
J
J
Roh
S
S
Kim
S R
SR
Min
K R
KR
Kim
C K
CK
Kim
E G
EG
Kim
Y
Y
D000421:Alcaligenes / Q000201:enzymology* / Q000235:genetics; D001713:Biphenyl Compounds / Q000378:metabolism*; D050557:Catechol 2,3-Dioxygenase; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D049308:Dioxygenases; D004591:Electrophoresis, Polyacrylamide Gel; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics; D015183:Restriction Mapping
D001713:Biphenyl Compounds; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D011078:Polychlorinated Biphenyls; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase