title: Human epidermal Langerhans cells express the high affinity receptor for immunoglobulin E (Fc epsilon RI).
abstract: It has been suggested that epidermal Langerhans cells (LC) bearing immunoglobulin E (IgE) may be involved in the genesis of atopic disease. The identity of the IgE receptor(s) on LC remained unclear, although it represents a crucial point in understanding cellular events linked to the binding of allergens to LC via IgE. In this report, we demonstrate that epidermal LC express the high affinity receptor for the Fc fragment of IgE (Fc epsilon RI) which has, so far, only been described on mast cells and basophils. Epidermal LC react with antibodies specific for the alpha subunit of the tetrameric (alpha, beta, 2 gamma) Fc epsilon RI. Specific transcripts for Fc epsilon RI alpha and Fc epsilon RI gamma were detected in LC and correspond to those of human basophils and of the human basophil cell line KU812. Furthermore, human basophils, KU812 cells, and LC express the putative beta subunit. Thus human LC express the complete structure of Fc epsilon RI. This finding opens new perspectives in the putative functional role of this structure on antigen-presenting cells.
authors.affiliation: Department of Dermatology, University of Munich Medical School, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Bieber
authors.forename: H
authors.initials: H
authors.lastname: de la Salle
authors.forename: A
authors.initials: A
authors.lastname: Wollenberg
authors.forename: J
authors.initials: J
authors.lastname: Hakimi
authors.forename: R
authors.initials: R
authors.lastname: Chizzonite
authors.forename: J
authors.initials: J
authors.lastname: Ring
authors.forename: D
authors.initials: D
authors.lastname: Hanau
authors.forename: C
authors.initials: C
authors.lastname: de la Salle
mesh_terms: D000595:Amino Acid Sequence; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001491:Basophils / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000378:metabolism*; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000166:cytology / Q000378:metabolism*; D008969:Molecular Sequence Data; D011961:Receptors, Fc / Q000235:genetics / Q000378:metabolism*; D017455:Receptors, IgE; D016415:Sequence Alignment
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D007141:Immunoglobulin Fc Fragments; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E; D004247:DNA