Southern molecular hybridization experiments with parallel complementary DNA probes.
We have detected the specific binding in Southern blot hybridization experiments of both complementary antiparallel and parallel 40 bp synthetic DNA probes, corresponding to a cloned Drosophila DNA fragment. The highly cooperative annealing and melting were observed in solution with these probes, which are complementary in the same direction and possess 17 GC pairs. The binding of ethidium bromide is indicative of formation of a perfect parallel DNA duplex. The specific binding was also detected in both genomic and in plaque hybridization experiments.
Tchurikov
N A
NA
Department of Genome Molecular Organization, V.A. Engelhardt Institute of Molecular Biology, Moscow, Russia.
Shchyolkina
A K
AK
Borissova
O F
OF
Chernov
B K
BK
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods*; D003001:Cloning, Molecular; D004247:DNA; D004331:Drosophila melanogaster / Q000235:genetics; D004996:Ethidium; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes
D015345:Oligonucleotide Probes; D004247:DNA; D004996:Ethidium