title: Effect of chronic maternal dietary magnesium deficiency on placental calcium transport.
abstract: Metabolisms of calcium (Ca) and magnesium (Mg) are closely interrelated in the intestine, bone, and kidney. Interaction of Ca and Mg at the placental level, however, is not well defined. The occurrence of decreased bone mineral content and hypocalcemia in infants of hypomagnesemic mothers led us to test the hypothesis that chronic dietary maternal Mg deficiency decreases placental Ca transport. On day 10 of gestation, 20 Sprague-Dawley rats were randomized to a Mg-deficient diet (3.3 mg/day, n = 10) or to a control diet (70 mg/day, n = 10). On day 20 of gestation (term = 22 days), intact placentas were perfused in situ through the umbilical artery and perfusate was collected through the umbilical vein. Calcium 45 (45Ca) and chromium 51-EDTA (51Cr-EDTA) (a diffusional marker for placental membrane integrity) were injected to the dam and steady state maternofetal clearance (Kmf45Ca, microliter/min/g placenta) of both isotopes were calculated. There was no difference in the clearance of 45Ca and 51Cr in both groups (55 +/- 10 vs 57 +/- 16 and 3.2 +/- 0.4 vs 3.6 +/- 0.4, respectively, mean +/- SEM). We conclude that, in the rat, placental Ca transfer is unaffected by chronic maternal dietary Mg deficiency. We speculate that Ca and Mg cross the placenta by independent mechanisms.
authors.affiliation: Department of Pediatrics, University of Cincinnati College of Medicine, OH 45267-0541.
authors.forename: M L
authors.initials: ML
authors.lastname: Cruz
authors.forename: F
authors.initials: F
authors.lastname: Mimouni
authors.forename: R C
authors.initials: RC
authors.lastname: Tsang
authors.forename: G
authors.initials: G
authors.lastname: Hammond
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000493:pharmacokinetics*; D002908:Chronic Disease; D005260:Female; D008275:Magnesium Deficiency / Q000378:metabolism*; D008431:Maternal-Fetal Exchange; D010477:Perfusion; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism*; D051381:Rats
chemical_list: D002118:Calcium