title: Search for MHC-associated genes in human: five new genes centromeric to HLA-DP with yet unknown functions.
abstract: Taking advantage of five mouse genomic or cDNA probes [KE5(probe 14), KE4 (probe 11), KE3 (probe 7), KE2 (probe 5), and SET] mapped on the H-2K region in mouse, we have identified and localized homologues of these five genes in the human major histocompatibility complex region (HKE5, HKE4, HKE3, HKE2, and HSET, respectively). Cosmid cloning and pulsed field gel electrophoresis analyses indicated that a human homologous gene, HKE5, is located 10 kilobases (kb) centromeric of the alpha 2 (XI) collagen (COL11A2) gene followed by HKE4. HKE3, closely linked to HKE2, is located 170 kb centromeric of HKE4. Furthermore, HSET is located 50 kb centromeric of HKE2. This gene organization outside the DP subregion is completely identical to that of the mouse H-2K region centromeric of I-Pb3, a mouse homologue of the DPB gene, except the lack of genes corresponding to the H-2K and -K2 genes in human.
authors.affiliation: Department of Transplantation II, Tokai University School of Medicine, Isehara, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Janatipour
authors.forename: Y
authors.initials: Y
authors.lastname: Naumov
authors.forename: A
authors.initials: A
authors.lastname: Ando
authors.forename: K
authors.initials: K
authors.lastname: Sugimura
authors.forename: N
authors.initials: N
authors.lastname: Okamoto
authors.forename: K
authors.initials: K
authors.lastname: Tsuji
authors.forename: K
authors.initials: K
authors.lastname: Abe
authors.forename: H
authors.initials: H
authors.lastname: Inoko
mesh_terms: D015139:Blotting, Southern; D002460:Cell Line; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D008040:Genetic Linkage / Q000235:genetics; D006183:H-2 Antigens / Q000235:genetics; D006682:HLA-DP Antigens / Q000235:genetics; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015342:DNA Probes; D006183:H-2 Antigens; D006682:HLA-DP Antigens