[BME 361 I--a new site-specific deoxyribonuclease type II from Bacillus megaterium 361].
Bme 361 I, a new site-specific type II deoxyribonuclease, was purified from Bacillus megaterium 361 by chromatography on phosphocellulose P 11 and hydroxylapatite. The enzyme recognizes and cleaves the nucleotide sequence 5'-GG decreases CC-3' in double-strand DNA. Thus it is a true isoschizomer of deoxyribonucleases Hae III and BspR I.
Lebedev
L R
LR
Andreeva
I S
IS
Serov
G D
GD
Denisova
L Ia
LIa
Pustoshilova
N M
NM
D001345:Autoradiography; D001410:Bacillus megaterium / Q000201:enzymology*; D010582:Bacteriophage lambda / Q000378:metabolism; D002853:Chromatography, Liquid; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D010957:Plasmids; D013379:Substrate Specificity
D004269:DNA, Bacterial; D004279:DNA, Viral; D003851:Deoxyribonucleases
BME 361 I--novaia saĭt-spetsificheskaia dezoksiribonukleaza II tipa iz Bacillus megaterium 361.