title: Effects of substitutions of glycine and asparagine for serine132 on activity and binding of human lipoprotein lipase to very low density lipoproteins.
abstract: For studying the role of Ser132 in the putative catalytic site of human lipoprotein lipase (LPL), mutant LPL cDNAs expressing LPLs with amino acid substitutions of Gly or Asn for Ser132 were obtained by site-directed mutagenesis, and were expressed in COS-1 cells. Considerable amounts of LPL enzyme protein mass were detected in the culture medium of COS-1 cells transfected with wild-type LPL, LPL-Gly132, or LPL-Asn132. LPL-Gly132 hydrolyzed Triton X-100-triolein and tributyrin as effectively as wild-type LPL, whereas LPL-Asn132 showed no activity. LPL-Asn132 bound to very low density lipoproteins as effectively as wild-type LPL.
authors.affiliation: Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Tashiro
authors.forename: J
authors.initials: J
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Shirai
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: I
authors.initials: I
authors.lastname: Fukamachi
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Nishida
authors.forename: T
authors.initials: T
authors.lastname: Shibui
authors.forename: Y
authors.initials: Y
authors.lastname: Morimoto
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001216:Asparagine / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002384:Catalysis; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D004789:Enzyme Activation; D005998:Glycine / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008071:Lipoprotein Lipase / Q000737:chemistry* / Q000235:genetics; D008079:Lipoproteins, VLDL / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D012694:Serine / Q000737:chemistry / Q000235:genetics*; D014304:Triolein
chemical_list: D008079:Lipoproteins, VLDL; D014304:Triolein; D012694:Serine; D001216:Asparagine; D008071:Lipoprotein Lipase; D005998:Glycine