title: Quantitation of Dam methyltransferase in Escherichia coli.
abstract: An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.
authors.affiliation: Department of Biophysics, Institute for Cancer Research, Oslo, Norway.
authors.forename: E
authors.initials: E
authors.lastname: Boye
authors.forename: M G
authors.initials: MG
authors.lastname: Marinus
authors.forename: A
authors.initials: A
authors.lastname: Løbner-Olesen
mesh_terms: D015153:Blotting, Western; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D007124:Immunoenzyme Techniques; D008780:Methyltransferases / Q000032:analysis* / Q000276:immunology; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
chemical_list: D029968:Escherichia coli Proteins; D008780:Methyltransferases; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C499294:dam protein, E coli