Localization of proteins forming the outer surface of isolated metaphase chromosomes.
The outer surface of isolated metaphase chromosomes has been investigated by a method of thermally activated tritium labelling. We show that both chromosomal proteins and DNA are tritium-labelled. Fractionation of the chromosomal proteins reveals that scaffold proteins are the most labelled in condensed and EDTA-decondensed chromosomes. Exposition of some scaffold proteins on the outer surface of metaphase chromosomes is suggested.
Belyaev
N D
ND
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences.
Budker
V G
VG
Dubrovskaya
V A
VA
Kim
A A
AA
Kiseleva
E V
EV
Sidorov
V N
VN
D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D002875:Chromosomes; D005347:Fibroblasts / Q000378:metabolism; D008677:Metaphase; D051379:Mice; D008854:Microscopy, Electron; D014316:Tritium
D002868:Chromosomal Proteins, Non-Histone; D014316:Tritium