title: [Preliminary studies of dose-effect relationship in overdose irradiation of electron beam with high energy--comparative observation of several indices of nuclear damage in lymphocyte of human peripheral blood].
abstract: In this paper we studied the relationship between doses (5-40Gy) and indices of nuclear damage in lymphocyte of human peripheral blood irradiated by 10 Mev of electron beam to explore possibility of establishing biodosimeter of overdose irradiation. The main results are as follows: (1) The frequency of micronucleated cell (MNCF), the frequency of irregular nucleus (INF), the frequency of pyknotic nucleus (PNF), the frequency of karyorrhetic nucleus (KNF) and the frequency of nuclear anomalies (NAF = MNCF + INF + PNF + KNF) increased along with increase of irradiation dose. Within the range of 0-20Gy indices of nuclear damages which were linear with irradiation doses are NAF (P less than 0.01), MNF (P less than 0.01), INF (P less than 0.05) and KNF (r = 0.8972), P greater than 0.05). Within the range of 0-40Gy the index of nuclear damage which was linear with irradiation dose was PNF (P less than 0.01) alone. (2) As compared with chromosome aberration analysis nuclear anomaly test is a more rapid and convenient assay, and can detect overdose radiation damage using chromosome analysis.
authors.affiliation: Jiangsu Institute of Cancer Research, Nanjian.
authors.forename: K
authors.initials: K
authors.lastname: Xue
authors.forename: S
authors.initials: S
authors.lastname: Wang
mesh_terms: D002467:Cell Nucleus / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004583:Electrons; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000528:radiation effects*