title: Antigen-specific tachycardia and hypotension in rodents.
abstract: Tachycardia and hypotension, two cardiovascular responses to anaphylaxis, were specifically induced by antigen in mice and rats, respectively. Intravenous injection of poly (Glu60Ala30Tyr10) (GAT) elicited tachycardia within 30-40 sec in GAT-primed B6 mice. Moreover, a minute amount of GAT (0.2 micrograms) was enough to sensitize the mice to subsequent GAT-induced tachycardia. Challenging doses ranging from 100 ng to 500 micrograms. could elicit tachycardia. The kinetics of tachycardia induction was different from that of antibody production or delayed-type hypersensitivity. Tachycardia was induced from day 6 after immunization, while delayed-type hypersensitivity developed as early as day 4, and anti-GAT antibodies were undetectable on day 6 and would not reach a maximum until day 8. Specific antigen-induced hypotension was also observed in rats. Furthermore, cardiovascular changes in both species could be passively transferred by heat-treated (56 degrees C, 30 min) sera from immunized animals. These benchmarks of antigen-induced cardiovascular changes in mice or rats could be used as models to study the immune control of cardiovascular changes in anaphylactic responses.
authors.affiliation: Department of Microbiology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Lei
authors.forename: H I
authors.initials: HI
authors.lastname: Chen
authors.forename: S H
authors.initials: SH
authors.lastname: Chan
authors.forename: S S
authors.initials: SS
authors.lastname: Leir
authors.forename: S B
authors.initials: SB
authors.lastname: Lin
authors.forename: L Y
authors.initials: LY
authors.lastname: Wing
mesh_terms: D000595:Amino Acid Sequence; D000707:Anaphylaxis / Q000209:etiology; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001794:Blood Pressure; D004562:Electrocardiography; D006968:Hypersensitivity, Delayed / Q000209:etiology; D007022:Hypotension / Q000209:etiology*; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D011108:Polymers; D051381:Rats; D011919:Rats, Inbred Strains; D013610:Tachycardia / Q000209:etiology*
chemical_list: D000941:Antigens; D010455:Peptides; D011108:Polymers; C020749:GAT