Stimulatory effect of free fatty acids on growth hormone releasing hormone secretion by fetal rat neurons in monolayer culture.
The effects of free fatty acids (FFAs) on growth hormone releasing hormone (GHRH) secretion by fetal rat cortical and hypothalamic neurons in monolayer culture have been investigated. Treatment with caprylic or oleic acids for 90 min, induced a dose-dependent increase in GHRH secretion. The stimulatory effect of caprylic acid was time-dependent, being present after 90 min and 5 h but not after 24 h. Finally, GHRH release induced by 56 mM KCl or by the calcium ionophore A23187 was not modified by 5 x 10(-3) M caprylic acid. These data support a role for FFAs on GHRH secretion.
Señaris
R M
RM
Department of Physiology, Faculty of Medicine, University of Santiago, Spain.
Lewis
M D
MD
Lago
F
F
Dominguez
F
F
Scanlon
M F
MF
Dieguez
C
C
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002210:Caprylates / Q000494:pharmacology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005333:Fetus; D013007:Growth Hormone-Releasing Hormone / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D007700:Kinetics; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats
D002210:Caprylates; D000001:Calcimycin; D011189:Potassium Chloride; D013007:Growth Hormone-Releasing Hormone; C031492:octanoic acid