In situ hybridization of PCR amplified inter-Alu sequences from a hybrid cell line.
Polymerase chain reaction amplified products promoted by oligonucleotides complementary to the highly repetitive human Alu sequence can be used for in situ hybridization on metaphase chromosomes to investigate the human content of a hybrid cell line. The TC65 primer, which combines the advantages of promoting the amplification of large inter-Alu sequences together with only small flanking Alu sequences, enables a simple and precise characterization to be made, with a high signal to noise ratio. Total human DNA is an efficient competitor in the removal of non-specific signals. The use of this oligonucleotide should be considered in the characterization of the human content of hybrids or in the generation of specific reagents for chromosome decoration.
Desmaze
C
C
URA 620 CNRS, Institut Curie, Paris, France.
Zucman
J
J
Delattre
O
O
Thomas
G
G
Aurias
A
A
D000818:Animals; D002460:Cell Line; D002892:Chromosomes, Human, Pair 22; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology; D007621:Karyotyping; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid
D009838:Oligodeoxyribonucleotides; D004247:DNA