title: A comparative study of the temperature dependence of the oxygen-binding properties of mammalian hemoglobins.
abstract: The effect of temperature on the oxygen-binding properties of hemoglobin (Hb) from ruminants, such as ox, reindeer, musk ox, mouflon and egyptian water buffalo is compared to that of human adult Hb (HbA). A striking difference emerges where in the presence of chloride ions and in the absence of 2,3-diphosphoglycerate [Gri(2,3)P2] a strongly reduced exothermic oxygenation process is observed for all ruminant Hb investigated with respect to HbA. Next, in the presence of physiological concentrations of Gri(2,3)P2, HbA displays a less exothermic oxygenation process, with values tending toward those observed in ruminant Hb [where Gri(2,3)P2 is not a physiological effector and for which the addition of Gri(2,3)P2 has essentially no effect on the oxygenation enthalpy]. Different from HbA, the intrinsically less exothermic oxygen binding seems to be independent of the experimental conditions for ruminant Hb, underlying specific structural characteristics which might be responsible for this feature.
authors.affiliation: Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Coletta
authors.forename: M E
authors.initials: ME
authors.lastname: Clementi
authors.forename: P
authors.initials: P
authors.lastname: Ascenzi
authors.forename: R
authors.initials: R
authors.lastname: Petruzzelli
authors.forename: S G
authors.initials: SG
authors.lastname: Condò
authors.forename: B
authors.initials: B
authors.lastname: Giardina
mesh_terms: D019794:2,3-Diphosphoglycerate; D000818:Animals; D002712:Chlorides / Q000378:metabolism; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D006441:Hemoglobin A / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008322:Mammals; D010100:Oxygen / Q000378:metabolism*; D010108:Oxyhemoglobins / Q000378:metabolism; D013696:Temperature; D013816:Thermodynamics
chemical_list: D002712:Chlorides; D004163:Diphosphoglyceric Acids; D006454:Hemoglobins; D010108:Oxyhemoglobins; D019794:2,3-Diphosphoglycerate; D006441:Hemoglobin A; D010100:Oxygen