title: Purification and characterization of a new member of the S-100 protein family from human placenta.
abstract: A novel Ca(2+)-binding protein which is termed S-100P was purified from human placenta with a hydrophobic column followed by an anion exchange column and reverse phase high performance liquid chromatography (HPLC). Molecular mass of the protein was 10 kDa according to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. Using immunoblotting technique, anti-human calcyclin antibodies did not bind to the S-100P. Isoelectric point of S-100P was pI = 4.6. S-100P did not formed disulfide-linked dimer. Calcium binding ability was proved by UV difference spectrometry, urea/alkaline gel electrophoresis, and 45Ca overlay technique. A ninety amino acid sequence of S-100P was determined. It is 49% identical with human S-100 beta, 38% with human calcyclin, and 37% with human cystic fibrosis antigen.
authors.affiliation: Department of Pharmacology, Nagoya University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Emoto
authors.forename: R
authors.initials: R
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Akatsuka
authors.forename: H
authors.initials: H
authors.lastname: Hidaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D002135:Calcium-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D009418:S100 Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
chemical_list: D002132:Calcium Radioisotopes; D002135:Calcium-Binding Proteins; D009418:S100 Proteins; D002118:Calcium