title: Cell polarity and calcium oxalate crystal adherence to cultured collecting duct cells.
abstract: The relationship between cell membrane polarity and calcium oxalate (CaOx) crystal binding was studied in rat renal inner medullary collecting duct (IMCD) cells in primary culture. Cultures grew as simple monolayers (M) with interspersed cellular aggregates (A), and CaOx bound preferentially to A. An antibody that recognizes an exclusively basolateral epitope in intact IMCD binds to some of the cells in A but not to cells in M. Lysing of intercellular junctions with 3 mM EGTA (monitored by transepithelial resistance, R) resulted in basolateral antibody binding to the previously negative cells in M and a 21-fold increase in CaOx adherence to M over control (P less than 0.01). Enhanced CaOx attachment appeared to lag behind the fall in R by 5-10 min. Crystal attachment returned to control between 30 and 120 min after removal of EGTA and readdition of Ca. These data suggest that loss of epithelial membrane polarity may result in enhanced capacity to bind CaOx. Such loss of cell membrane polarity may occur in IMCD with some forms of epithelial injury and repair and may provide a site of crystal fixation to initiate nephrolithiasis.
authors.affiliation: Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295.
authors.forename: R J
authors.initials: RJ
authors.lastname: Riese
authors.forename: N S
authors.initials: NS
authors.lastname: Mandel
authors.forename: J H
authors.initials: JH
authors.lastname: Wiessner
authors.forename: G S
authors.initials: GS
authors.lastname: Mandel
authors.forename: C G
authors.initials: CG
authors.lastname: Becker
authors.forename: J G
authors.initials: JG
authors.lastname: Kleinman
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002129:Calcium Oxalate; D002449:Cell Aggregation; D016764:Cell Polarity; D002478:Cells, Cultured; D003460:Crystallization; D004533:Egtazic Acid / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007685:Kidney Tubules, Collecting / Q000166:cytology*; D008297:Male; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D002129:Calcium Oxalate; D004533:Egtazic Acid