A codon sugar structure strongly influences tRNA binding to programmed ribosomes.
To study the role of a codon sugar-phosphate backbone in aminoacyl-tRNA selection on the ribosome a comparison of tRNA(Phe) affinity for pdTpdTpdT and prUprUprU in solution, and for correspondingly programmed 30S ribosomal subunits has been performed. In solution the tRNA(Phe) affinity for pdTpdTpdT appeared to be even slightly higher than for prUprUprU, whereas deoxyribocodon was significantly less efficient in the stimulation of Phe-tRNA(Phe) binding to the 30S ribosomal subunit. Some difference in neomycin action in both systems was revealed.
Soldatkin
K O
KO
Institute of Molecular Biology and Genetics, Kiev, Ukraine.
Koval'chuke
O
O
Potapov
A P
AP
El'skaya
A V
AV
D000926:Anticodon / Q000187:drug effects / Q000378:metabolism; D002236:Carbohydrate Conformation; D003062:Codon / Q000187:drug effects / Q000378:metabolism; D009355:Neomycin / Q000494:pharmacology; D011071:Poly T / Q000378:metabolism*; D011072:Poly U / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000187:drug effects / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*
D000926:Anticodon; D003062:Codon; D012360:RNA, Transfer, Phe; D011071:Poly T; D011072:Poly U; D009355:Neomycin