title: Simultaneous patient-side measurement of hemoglobin, glucose, and cholesterol in finger-stick blood.
abstract: We describe a multianalyte assay system for patient-side use comprising single-use plastic cartridges and a small monitor. Hemoglobin, glucose, and cholesterol can be simultaneously measured in 3 min in an unmeasured volume of blood. The sample is drawn by capillary action into four channels for delivery to assay-specific stacks containing a set of closely apposed layers. The distal layer is a membrane that acts as the optical surface for reflectance optics. For glucose and cholesterol assays, erythrocytes are removed by a fibrous filter layer and oxidase-peroxidase chemical reactions contained in the optical membrane generate a colored product. For hemoglobin measurement, blood is lysed by detergent contained in a porous disk. The amount of color reaching the optical membrane is measured by fiber optics. To ensure fail-safe operation, sensors verify sample sufficiency and degree of hemolysis. The assays perform comparably with laboratory methods.
authors.affiliation: Biotrack, Ciba Corning Diagnostics, Mountain View, CA 94043.
authors.forename: B
authors.initials: B
authors.lastname: Sterling
authors.forename: T
authors.initials: T
authors.lastname: Kiang
authors.forename: K
authors.initials: K
authors.lastname: Subramanian
authors.forename: M
authors.initials: M
authors.lastname: Saltman
authors.forename: W
authors.initials: W
authors.lastname: Smart
authors.forename: M
authors.initials: M
authors.lastname: Tsay
authors.forename: J
authors.initials: J
authors.lastname: Sugarman
authors.forename: D
authors.initials: D
authors.lastname: Patel
authors.forename: D
authors.initials: D
authors.lastname: Monger
authors.forename: D
authors.initials: D
authors.lastname: Martin
mesh_terms: D001786:Blood Glucose / Q000032:analysis*; D002784:Cholesterol / Q000097:blood*; D003902:Detergents; D004912:Erythrocytes / Q000737:chemistry; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D010088:Oxidoreductases / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D010783:Photometry / Q000379:methods
chemical_list: D001786:Blood Glucose; D003902:Detergents; D006454:Hemoglobins; D002784:Cholesterol; D010088:Oxidoreductases; D010544:Peroxidases