Cloning of the 62-kilodalton component of basic transcription factor BTF2.
Cloning of the mammalian basic transcription factors serves as a major step in understanding the mechanism of transcription initiation. The 62-kilodalton component (p62) of one of these transcription factors, BTF2 was cloned and overexpressed. A monoclonal antibody to this polypeptide inhibited transcription in vitro. Immunoaffinity experiments demonstrated that the 62-kilodalton component is closely associated with the other polypeptides present in the BTF2 factor. Sequence similarity suggests that BTF2 may be the human counterpart of RNA polymerase II initiation factor b from yeast.
Fischer
L
L
Laboratoire de Génétique Moléculaire des Eucaryotes, Centre National de la Recherche Scientifique, Unité 184, Faculté de Médecine, Strasbourg, France.
Gerard
M
M
Chalut
C
C
Lutz
Y
Y
Humbert
S
S
Kanno
M
M
Chambon
P
P
Egly
J M
JM
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH