The murine local lymph node assay: a commentary on collaborative studies and new directions.
The murine local lymph node assay is a predictive test for the identification of contact allergens. This paper provides a historical background to the development of the assay and describes the performance of a recently completed interlaboratory trial designed to evaluate further the utility of the method as an alternative or adjunct to guinea-pig predictive tests. On the basis of these and supplementary investigations, a number of recommendations can be made regarding the use and interpretation of the local lymph node assay. Finally, a number of issues arising from recent studies are discussed, including comparisons of the local lymph node assay with guinea-pig methods.
Kimber
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ICI Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, UK.
Basketter
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D000485:Allergens / Q000633:toxicity*; D000818:Animals; D003877:Dermatitis, Contact; D008198:Lymph Nodes / Q000187:drug effects*; D051379:Mice; D008808:Mice, Inbred CBA
D000485:Allergens