Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31.
The chromosomal localizations of two closely related human DNA repair genes, HHR6A and HHR6B, were determined by in situ hybridization with biotinylated probes. HHR6A and HHR6B (human homolog of yeast RAD6) encode ubiquitin-conjugating enzymes (E2 enzymes), likely to be involved in postreplication repair and induced mutagenesis. The HHR6B gene was assigned to human chromosome 5q23-q31, whereas the HHR6A gene was localized on the human X chromosome (Xq24-q25). This latter assignment was confirmed with an X-specific human-mouse/hamster somatic cell hybrid panel. Southern blot analysis points to an X and an autosomal localization of HHR6A and HHR6B, respectively, in the mouse. The potential involvement of these genes in human genetic disorders is discussed.
Koken
M H
MH
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Smit
E M
EM
Jaspers-Dekker
I
I
Oostra
B A
BA
Hagemeijer
A
A
Bootsma
D
D
Hoeijmakers
J H
JH
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D004260:DNA Repair / Q000235:genetics*; D030342:Genetic Diseases, Inborn / Q000235:genetics; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology / Q000502:physiology; D008025:Ligases / Q000235:genetics*; D051379:Mice; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D044763:Ubiquitin-Conjugating Enzymes; D014960:X Chromosome
D015342:DNA Probes; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; C471606:RAD6 protein, S cerevisiae; C471545:UBE2A protein, human; C471546:UBE2B protein, human; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases