An enzyme-linked immunosorbent assay for the detection of human seminal plasma inhibin.
A simple and sensitive enzyme-linked immunosorbent assay (ELISA) is described for the measurement of inhibin in urine and seminal plasma. Standards used cover a range from 50 ng to 0.05 ng/0.1 ml with a detection limit of 0.098 ng/0.1 ml. Coefficients of variation for intra-assay precision and for inter-assay precision were obtained and compared favourably with RIA. Given the ease of application, this technique is an useful alternative to existing radioimmuno-assays for this peptide.
Jose
S M
SM
Institute for Research in Reproduction (ICMR), Parel, Bombay, India.
Moodbidri
S B
SB
Sheth
A R
AR
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006801:Humans; D007265:Inhibins / Q000032:analysis* / Q000592:standards / Q000652:urine; D008297:Male; D011863:Radioimmunoassay; D012015:Reference Standards; D012661:Semen / Q000737:chemistry*; D012680:Sensitivity and Specificity
D007265:Inhibins