Topographical investigations of human ovarian-carcinoma polymorphic epithelial mucin by scanning tunnelling microscopy.
Human polymorphic epithelial mucin is a high-molecular-mass glycoprotein that associates to provide protection to the epithelial-cell surface and may afford the malignant cell a selective advantage for growth. The scanning-tunnelling-microscopy micrographs obtained in the present study identify the purified human ovarian-carcinoma polymorphic epithelial mucin glycoproteins as rod-shaped molecules of mixed length. The dimensions of the individual molecules range from 25 to 45 nm in length and are 3-4 nm in width. The images further suggest that lateral association of the rods occurs.
Roberts
C J
CJ
VG STM Laboratory for Biological Applications, Department of Pharmaceutical Sciences, Nottingham, U.K.
Sekowski
M
M
Davies
M C
MC
Jackson
D E
DE
Price
M R
MR
Tendler
S J
SJ
D000818:Animals; D000911:Antibodies, Monoclonal; D001202:Ascitic Fluid / Q000737:chemistry; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000648:ultrastructure* / Q000652:urine; D051379:Mice; D016252:Microscopy, Scanning Tunneling / Q000379:methods; D015394:Molecular Structure; D018396:Mucin-1; D009077:Mucins / Q000302:isolation & purification / Q000648:ultrastructure* / Q000652:urine; D010051:Ovarian Neoplasms / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate
D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D018396:Mucin-1; D009077:Mucins; D012967:Sodium Dodecyl Sulfate