Structural organization of two fast, rhythmically active crustacean muscles.
The organization of the flagellum abductor muscle and of a scaphognathite levator muscle of the green crab, Carcinus maenas, has been compared quantitatively using light and electron microscopy. These muscles are rhythmically active at relatively high frequencies and for long durations. Fibers of both muscles are interconnected to form fascicles of 50 or more fibers within which there is cytoplasmic continuity. A single muscle is made up of 8-12 fascicles. Individual fibers consist of a peripheral rind of densely packed mitochondria, a thick region of glycogen granules, and myofibrils arranged into scattered central islands. Less than half the volume-density of these muscles is contractile material, the balance being largely mitochondria and glycogen. The fibers within a muscle are structurally similar. They have short sarcomeres (about 2 microns), thin to thick filament ratios of about 3:1, and junctions between the sarcoplasmic reticulum and the transverse tubules at the M line. Sarcoplasmic reticulum occupies about 10% of the myofibrillar volume-density. A well developed sarcoplasmic reticulum appears to underlie the capacities of these two muscles for high frequency contraction; extensive mitochondria and glycogen stores should confer fatigue resistance under both aerobic and anaerobic conditions.
Stokes
D R
DR
Department of Biology, Emory University, Atlanta, GA 30322.
Josephson
R K
RK
D000818:Animals; D003386:Brachyura / Q000033:anatomy & histology*; D006003:Glycogen / Q000032:analysis; D008297:Male; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D009132:Muscles / Q000033:anatomy & histology* / Q000737:chemistry / Q000648:ultrastructure; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure
D006003:Glycogen