title: Type II and type IX collagen form heterotypic fibers in the tectorial membrane of the inner ear.
abstract: The presence of type II and IX collagen in the adult gerbil inner ear was probed by use of preembedding and post-embedding immunocytochemistry. Monoclonal antibodies to type II and IX collagen both label the tectorial membrane, an acellular structure which lies over the cochlear sensory hair cells and plays an essential role in the transduction process. At the light microscopic level, the antibodies are localized throughout the tectorial membrane. At the electron microscopic level, antibodies against types II and IX collagen are co-localized over the thick unbranched (Type A) radial fibers but not over the thin highly branched (Type B) fibers in which the thick fibers are embedded. Thus, the tectorial membrane of the cochlea represents another non-cartilaginous structure in which type II and IX collagen are present and arranged in heterotypic fibers. The organization of these fibers into bundles, meshworks and layers results in the formation of a structure with the unique properties necessary to withstand mechanical stresses associated with sensory transduction.
authors.affiliation: Institute for Sensory Research, Syracuse University, NY.
authors.forename: N B
authors.initials: NB
authors.lastname: Slepecky
authors.forename: L K
authors.initials: LK
authors.lastname: Cefaratti
authors.forename: T J
authors.initials: TJ
authors.lastname: Yoo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003094:Collagen / Q000032:analysis* / Q000145:classification / Q000276:immunology / Q000648:ultrastructure; D005849:Gerbillinae; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D013314:Stress, Mechanical; D013680:Tectorial Membrane / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D003094:Collagen