title: Induction of myogenic differentiation in human rhabdomyosarcoma cells by ionising radiation, N,N-dimethylformamide and their combination.
abstract: Differentiation-inducing ability of gamma-radiation, N,N-dimethylformamide and their combination has been tested on human rhabdomyosarcoma RMZ-RC2 clone cells. Ionising radiation at 2-5 Gy doses induced a more differentiated morphology, with the appearance of an increased proportion of multinuclear myotube-like cells, and a significant increase in myosin-positive and multinuclear cells. Radiation appeared to act by inducing de novo differentiated elements. N,N-dimethylformamide was able to induce an increased myosin expression, but did not affect multinuclear cell proportion. The combined treatment (ionising radiation and N,N-dimethylformamide) resulted in an additive increase in the proportion of myosin-positive cells, approaching 25-35%, but de novo differentiated elements were not increased above the levels obtained with irradiation alone.
authors.affiliation: Institute of Cancerology, University of Bologna, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Nicoletti
authors.forename: C
authors.initials: C
authors.lastname: De Giovanni
authors.forename: L
authors.initials: L
authors.lastname: Landuzzi
authors.forename: G
authors.initials: G
authors.lastname: Simone
authors.forename: P
authors.initials: P
authors.lastname: Rocchi
authors.forename: P
authors.initials: P
authors.lastname: Nanni
authors.forename: P L
authors.initials: PL
authors.lastname: Lollini
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects; D004126:Dimethylformamide / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D009132:Muscles / Q000166:cytology*; D011650:Pulmonary Alveoli / Q000473:pathology; D012208:Rhabdomyosarcoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D004126:Dimethylformamide