Isolation and bioactivity of putative cholecystokinin-releasing peptide from rat small intestinal mucosa.
Pancreatic exocrine secretion in the conscious rat is regulated by proteases in the intestine secreted by the pancreas, and cholecystokinin (CCK) is known to be involved in the mechanism. The authors proposed that the release of CCK was regulated by a CCK-releasing factor secreted into the intestinal lumen from the proximal intestine. We isolated and partially purified a CCK-releasing factor from rat small intestine by gel filtration and high performance liquid chromatography. The partially purified CCK-releasing factor increased pancreatic exocrine secretions and plasma CCK concentrations in conscious rats and this activity was abolished after the incubation with trypsin. The bioactivity of the partially purified CCK-releasing factor was confirmed.
Miyasaka
K
K
Department of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.
Funakoshi
A
A
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004386:Duodenum / Q000378:metabolism*; D005246:Feedback / Q000502:physiology; D005768:Gastrointestinal Hormones / Q000302:isolation & purification* / Q000378:metabolism*; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000302:isolation & purification* / Q000378:metabolism*
D005768:Gastrointestinal Hormones; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; C504705:Spink1 protein, rat; D014359:Trypsin Inhibitor, Kazal Pancreatic