title: Formation of stable p53 homotetramers and multiples of tetramers.
abstract: We have used gradient gel electrophoresis and chemical cross-linking to analyze the quaternary structure of purified, wild-type, murine p53. Under nondenaturing conditions, p53 electrophoreses as tetramers and multiples of tetramers. Under denaturing conditions, fully cross-linked p53 also behaves as tetrameric structures. We confirmed the composition of the tetramers by partially cross-linking p53 and dissociating tetramers into monomers, dimers, and trimers.
authors.affiliation: Department of Microbiology, State University of New York, Stony Brook 11794.
authors.forename: J E
authors.initials: JE
authors.lastname: Stenger
authors.forename: G A
authors.initials: GA
authors.lastname: Mayr
authors.forename: K
authors.initials: K
authors.lastname: Mann
authors.forename: P
authors.initials: P
authors.lastname: Tegtmeyer
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008970:Molecular Weight; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D016159:Tumor Suppressor Protein p53