Immunoglobulin idiotype expression in reactive lymphoid tissues and B-cell lymphomas.
In an investigation of the immunoglobulin idiotypic expression of non-tumour and neoplastic B lymphocytes in situ, fresh-frozen specimens of reactive tonsils, lymph nodes and B-cell malignant lymphomas (B-MLs) from Japanese patients were studied immunohistochemically with 39 different anti-idiotype antibodies. In reactive lymphoid tissues, while idiotype-bearing cells were largely distributed sparsely in follicles and perifollicular areas, some were heavily crowded in particular germinal centres (GCs), suggesting the presence of oligoclonal proliferations of B-cells in GCs. Forty-eight out of 100 B-MLs reacted with anti-idiotype antibodies. This proportion was significantly higher than those reported in Western cases (27-36%), indicating that Japanese B-MLs share public idiotypes much frequently than western cases. The idiotypes demonstrated in these lymphomas, in contrast to those not expressed in any B-ML, were found much commonly in non-tumour lymphocytes, suggesting that such public idiotypes as were common in B-MLs were frequently shared by normal B-lymphocytes.
Shiota
M
M
Department of Pathology, University of Tokyo, Japan.
Kitamura
K
K
Noda
M
M
Kawanishi
Y
Y
Hart
S
S
Miller
R A
RA
Mori
S
S
D000906:Antibodies; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D006801:Humans; D006965:Hyperplasia; D007124:Immunoenzyme Techniques; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D008198:Lymph Nodes / Q000276:immunology* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000276:immunology* / Q000473:pathology; D014066:Palatine Tonsil / Q000276:immunology* / Q000473:pathology
D000906:Antibodies; D007130:Immunoglobulin Idiotypes