Molecular cloning and expression of hsp82 gene of the dimorphic pathogenic fungus Histoplasma capsulatum.
We have cloned a nucleotide sequence from Histoplasma capsulatum G222B corresponding to a heat inducible hsp82 gene, and determined its entire sequence and the flanking regions. During the temperature-controlled mycelium-to-yeast phase transition the gene is more actively transcribed at 37 degrees C in the temperature tolerant and mouse-virulent G222B strain, while 34 degrees C is the optimum for transcription in the temperature sensitive and mouse-avirulent Downs strain.
Minchiotti
G
G
International Institute of Genetics and Biophysics, CNR, Naples, Italy.
Gargano
S
S
Maresca
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000096:biosynthesis*; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics*; D006658:Histoplasma / Q000235:genetics*; D008969:Molecular Sequence Data; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; C074510:HSP82 protein, S cerevisiae; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins