Mucosal immunity to Pseudomonas aeruginosa alginate in cystic fibrosis.
Patients with cystic fibrosis commonly acquire chronic pulmonary infection with alginate-producing Pseudomonas aeruginosa. The infection remains localized at the mucosal surfaces of the airways. Using enzyme-linked immunosorbent assays immunoglobulin concentrations and titers of specific antibodies to purified P. aeruginosa alginate and to P. aeruginosa sonicated antigens were measured in tears, saliva, sputum and serum. CF patients had significantly higher concentrations of IgG, IgA and SIgA in serum and saliva than controls. They also had significantly higher levels of specific antibodies to alginate and sonicated antigen in secretions and serum. Local production of IgA, IgG and IgM antibodies to P. aeruginosa was demonstrated. Only a minor proportion of specific IgA antibodies were present as secretory IgA in tears, saliva and sputum. The ratio of alginate-specific SIgA to specific monomeric IgA in sputum was significantly lower than the similar ratio in saliva, whereas the same ratio for specific P. aeruginosa sonicate antigens was found in saliva and sputum.
Pedersen
S S
SS
Statens Seruminstitut, Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
Møller
H
H
Espersen
F
F
Sørensen
C H
CH
Jensen
T
T
Høiby
N
N
D000293:Adolescent; D000328:Adult; D000464:Alginates; D000907:Antibodies, Bacterial / Q000378:metabolism*; D000942:Antigens, Bacterial / Q000276:immunology*; D003550:Cystic Fibrosis / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D008168:Lung / Q000276:immunology*; D009092:Mucous Membrane / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D012463:Saliva / Q000276:immunology; D013666:Tears / Q000276:immunology
D000464:Alginates; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory