The DM20 protein of myelin: intracellular and surface expression patterns in transfectants.
DM20 is an abundant CNS myelin-specific protein whose role in myelinogenesis is unknown. We have cloned the DM20 cDNA from adult mouse brain total RNA using the polymerase chain reaction and expressed it in HeLa cells. DM20, detected by immunofluorescence in stable transfectants, is present in some cells in large, intensely fluorescent intracellular clumps that probably represent elements of the rough endoplasmic reticulum and Golgi apparatus. Frequently, intense DM20 fluorescence could be detected at the plasma membrane. These findings are consistent with previous studies demonstrating that an intracellular "pool" of DM20 and its larger isoform, proteolipid protein, exists and that a substantial lag occurs between synthesis and insertion of these proteins into the expanding myelin membrane. Permanent DM20 expressors in contact with one another do not display any ultrastructural rearrangements at regions of cell-cell contact, in contrast to what we have previously reported for P0, a PNS-specific protein shown to mediate adhesion of the extracellular faces of the Schwann cell during PNS myelinogenesis. We believe that these results indicate that if DM20 is indeed an adhesion molecule, this property is likely to be significantly more subtle than P0-mediated adhesion.
Timsit
S
S
Hôpital de la Salpêtrière, INSERM U. 134, Paris, France.
Sinoway
M P
MP
Levy
L
L
Allinquant
B
B
Stempak
J
J
Staugaitis
S M
SM
Colman
D R
DR
D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000378:metabolism*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D018991:Myelin Proteolipid Protein; D011510:Proteolipids / Q000378:metabolism*; D014018:Tissue Distribution; D014162:Transfection
D018991:Myelin Proteolipid Protein; D011510:Proteolipids