Assessment of neonatal avian inflammatory macrophage function following embryonic cyclophosphamide exposure.
Inflammatory macrophage recruitment and function were examined in 4-5-week-old chickens which had received two doses of cyclophosphamide (CP) or vehicle (dH2O) during late embryogenesis (18 and 19 days of incubation). Mononuclear leukocyte chemotaxis to N-formyl-methionyl-leucyl-phenylalanine (FMLP) and hematological parameters were unchanged in CP-treated vs control chickens. Peritoneal exudate cells (PECs) elicited in response to intraperitoneal (i.p.). Sephadex injection did not differ in CP-treated vs control chickens with respect to cell number, cell type, superoxide anion production, or cell surface expression of Ia and transferrin receptor (TfR) antigens. The CP-treated chickens did exhibit the expected decrease in bursa weight; male chickens exposed to CP also had inhibited testes growth. Although embryonic exposure to CP at this dose results in irreversible bursal damage and subsequent impaired humoral immunity, it appears that there are no long-lasting effects on avian inflammatory macrophage function.
Golemboski
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Department of Microbiology, Immunology and Parasitology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
Bloom
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Dietert
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D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D001835:Body Weight / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002642:Chick Embryo / Q000187:drug effects*; D002645:Chickens; D003520:Cyclophosphamide / Q000633:toxicity*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007958:Leukocyte Count / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011990:Receptors, Transferrin / Q000032:analysis
D000949:Histocompatibility Antigens Class II; D011990:Receptors, Transferrin; D003520:Cyclophosphamide