Characterization of a Xenopus oocyte factor that binds to a developmentally regulated cis-element in the TFIIIA gene.
TFIIIA mRNA expression is developmentally regulated during Xenopus oogenesis. We have identified a positive-acting cis-element in the TFIIIA gene located between -671 and -629 with respect to the mRNA initiation site, termed element 3. Element 3 contributes to the efficient transcription of the TFIIIA gene in stage III oocytes, but is inactive in the normal context of the TFIIIA gene in stage VI oocytes. A trans-acting factor, termed B3, in immature ovary extracts binds to a region within element 3 containing four repeats of the consensus nucleotide sequence 5' (T/A)GGTTACT. We discuss how the developmental regulation of B3-element 3 function could be achieved in the context of the TFIIIA gene during oogenesis.
Pfaff
S L
SL
Department of Molecular Physiology and Biophysics, School of Medicine, Vanderbilt University, Nashville, Tennessee 37232-0615.
Taylor
W L
WL
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D005260:Female; D008969:Molecular Sequence Data; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism*; D009866:Oogenesis / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D015534:Trans-Activators; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*; D014981:Xenopus / Q000196:embryology / Q000235:genetics*
D004268:DNA-Binding Proteins; D015534:Trans-Activators; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors