Mutagenicity of benzo(a)pyrenyl-1-sulfate in the Ames test.
Comparison of the mutagenicity of nine isomeric benzo(a)pyrenyl [B(a)P] phenols conjugated with either sulfate or glucuronide was carried out using strain Salmonella typhimurium TA98. Of the nine conjugates tested, only B(a)P-1-sulfate was mutagenic. Accordingly, the mutagenicity of B(a)P-1-sulfate was compared with that of B(a)P and 1-hydroxybenzo(a)pyrene [B(a)P-1-OH] in the presence and absence of rat lung S9 and Aroclor-induced liver S9 with and without an NADPH-generating system. B(a)P-1-sulfate was slightly mutagenic, whereas B(a)P and the 1-hydroxy derivative were nonmutagenic when S9 fractions and NADPH were omitted. Addition of induced liver S9 with NADPH caused mutagenicity with B(a) -1-OH greater than B(a)P greater than B(a)P-1-sulfate. B(a)P-1-sulfate was the only mutagenic species when lung S9 was added. This mutagenicity did not require NADPH. Sodium sulfite, an inhibitor of arylsulfatase, decreased the mutagenicity of B(a)P-1-sulfate. These data suggest that a unique mutagenic species is generated from B(a)P-1-sulfate via arylsulfatase in rat lung.
Irwin
S E
SE
Laboratory for Cellular and Biochemical Toxicology, Rutgers University, New Brunswick, New Jersey.
Kwei
G Y
GY
Blackburn
G R
GR
Thurman
R
R
Kauffman
F C
FC
D000818:Animals; D001192:Arylsulfatases / Q000037:antagonists & inhibitors; D001580:Benzopyrenes / Q000378:metabolism / Q000633:toxicity*; D001711:Biotransformation; D005965:Glucuronates / Q000633:toxicity; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009249:NADP / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects; D013431:Sulfates / Q000633:toxicity; D013447:Sulfites / Q000494:pharmacology
D001580:Benzopyrenes; D005965:Glucuronates; D009153:Mutagens; D013431:Sulfates; D013447:Sulfites; D009249:NADP; C074216:benzo(a)pyrenyl-1-sulfate; D001192:Arylsulfatases; C025026:sodium sulfite