Hemagglutination and graft-versus-host disease in the severe combined immunodeficiency mouse lymphoproliferative disease model.
In the course of evaluating the severe combined immunodeficiency mouse-human peripheral blood lymphocyte (SCID-PBL) model of lymphoproliferative disease, we noted hemagglutination occurring in peripheral blood smears of mice with serum human immunoglobulin levels greater than 1.0 mg/ml. The hemagglutinating process was mediated by human anti-mouse red cell antibodies of the IgM class, peaked at five to seven weeks post-transfer of 5 to 7 x 10(7) human PBL and was generally self limiting. However, death resulted in some mice when serum immunoglobulin levels were greater than 3.0 mg/ml. The most severely affected mice had hemagglutination induced congestion of liver, lungs and spleen. Several mice also had lesions consistent with graft-versus-host disease (GVHD) including focal hepatic necrosis and destruction of mouse splenic hematopoietic elements. The lesions associated with hemagglutination and GVHD in SCID-PBL mice are distinct from those associated with EBV-induced lymphoproliferation. Recognition of these pathologic processes are required for a thorough understanding of the SCID-PBL model.
Pirruccello
S J
SJ
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-6495.
Nakamine
H
H
Beisel
K W
KW
Kleveland
K L
KL
Okano
M
M
Taguchi
Y
Y
Davis
J R
JR
Mahloch
M L
ML
Purtilo
D T
DT
D000818:Animals; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000378:metabolism; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000209:etiology* / Q000473:pathology; D006384:Hemagglutination; D007136:Immunoglobulins / Q000097:blood; D008232:Lymphoproliferative Disorders / Q000097:blood* / Q000150:complications / Q000473:pathology; D051379:Mice; D016513:Mice, SCID; D016511:Severe Combined Immunodeficiency / Q000097:blood* / Q000150:complications / Q000473:pathology
D007136:Immunoglobulins