Culture techniques for human keratinocytes.
The two main approaches developed for in vitro culture of human keratinocytes are reviewed and discussed. The older technique is based on the use of a serum-containing medium and of a feeder-layer of lethally irradiated mouse fibroblasts; the second relies upon serum-free media, in the absence of a feeder-layer. While the former technique is most widely used for the preparation of epithelial sheets for grafting, the latter is best suited for studies on the biological properties of keratinocytes.
Tenchini
M L
ML
Dipartimento di Biologia e Genetica per le Scienze mediche, dell'Università degli Studi di Milano, Italy.
Ranzati
C
C
Malcovati
M
M
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D012867:Skin / Q000166:cytology / Q000473:pathology; D016038:Skin Transplantation / Q000379:methods