Adhesion of mutants streptococci to glass with and without a salivary coating as studied in a parallel-plate flow chamber.
Deposition and adhesion to glass with and without a salivary coating in a parallel-plate flow chamber were studied with four strains of mutans streptococci. Stationary-state adhesion of the strains to uncoated glass ranged from 0.3 x 10(6) cm-2 (Streptococcus rattus HG218) to 12.7 x 10(6) cm-2 (Streptococcus sobrinus HG1025) and generally decreased after saliva coating of the glass. The poor adhesion found for S. rattus HG218 to both uncoated and saliva-coated glass could be due to its relatively high negative surface-charge. Deposition efficiencies of all strains were greater than or equal to 1 for uncoated glass and decreased greatly after saliva coating of the glass. Possibly, adhesion to a saliva coating is less efficient and more time-consuming than that to uncoated glass, because stereochemical groups in the pellicle and on the cell surfaces may have to re-arrange before an effective interaction can occur. Desorption rates, measured 1000 s and 5000 s after the start of an experiment, decreased by a factor of ten upon a five-fold increase in contact time, indicative of a two-phase adhesion process. Of the four strains studied, only Streptococcus cricetus HG737 showed a minor positive cooperativity on saliva-coated glass, possibly mediated by surface appendages observed by transmission electron microscopy on negatively-stained cells. Retention of adhering bacteria was strain-dependent on uncoated glass, but was identical for all strains on saliva-coated glass, which suggests that the structure and composition of the pellicle may be more important with respect to the retention of adhering cells than the cell-surface properties themselves.
Busscher
H J
HJ
Laboratory for Materia Technica, University of Groningen, The Netherlands.
Doornbusch
G I
GI
Van der Mei
H C
HC
D001422:Bacterial Adhesion / Q000502:physiology*; D015169:Colony Count, Microbial; D005260:Female; D005898:Glass; D006801:Humans; D007700:Kinetics; D008297:Male; D008858:Microscopy, Phase-Contrast; D012212:Rheology; D012463:Saliva / Q000502:physiology*; D013291:Streptococcus / Q000502:physiology*; D013295:Streptococcus mutans / Q000502:physiology*; D013499:Surface Properties