title: Comparison of histomorphometric values in iliac trabecular bone of beagle dogs raised under different breeding systems.
abstract: In order to clarify individual differences in bone metabolism among colony-raised beagle dogs, histomorphometric values of iliac trabecular bone and values of serum biochemical constituents related to bone were examined in 10 and 17 beagle dogs raised, respectively, under our two breeding systems in which differences in factors such as exercise, ultraviolet rays, and mineral content of the diet affect bone metabolism. At the age of 14 months, all dogs were injected with tetracycline hydrochloride and calcein twice for double bone labeling in order to measure dynamic as well as static parameters by bone histomorphometry and the ilium was later biopsied. The measurement on cancellous bone areas of undecalcified iliac sections was performed with a semiautomatic image analyser. Values of total calcium, phosphorus, alkalinephosphatase activity, parathyroid hormone and calcitonin in serum were also determined. The results showed that there were no significant differences between the two groups in histomorphometric values, except for the osteoid volume (p less than 0.05) and osteoid surface/trabecular surface ratio (p less than 0.01) in females, or in serum biochemical constituents, except for alkalinephosphatase activity (p less than 0.001) in males, indicating there were virtually no individual differences in bone metabolism in normal colony raised beagle dogs.
authors.affiliation: Division of Comparative Radiotoxicology, National Institute of Radiological Sciences, Chiba, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Fukuda
authors.forename: H
authors.initials: H
authors.lastname: Iida
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001947:Breeding; D002116:Calcitonin / Q000097:blood; D002118:Calcium / Q000097:blood; D004285:Dogs; D005260:Female; D007085:Ilium / Q000033:anatomy & histology* / Q000378:metabolism; D008297:Male; D010281:Parathyroid Hormone / Q000097:blood; D010758:Phosphorus / Q000097:blood
chemical_list: D010281:Parathyroid Hormone; D010758:Phosphorus; D002116:Calcitonin; D000469:Alkaline Phosphatase; D002118:Calcium