title: Doxorubicin interacts directly with skinned single skeletal muscle fibres.
abstract: The effect of doxorubicin, a highly effective anticancer agent, on the contractile apparatus of skinned single muscle fibres was tested in a concentration of 1 microM. Sarcomere length was set and held at 2 microns. Doxorubicin induced an increase in tension dependent on the Ca2+ concentration and time of incubation. The rise was up to 25% at [Ca2+] 40 microM. A parallel, small but significant shift of the calcium sensitivity curve, the relation between normalized tension and the negative logarithm of [Ca2+], the pCa, was observed. The results of this study suggest a direct interaction of doxorubicin with the actin myosin structure, possibly by an effect on myosin-ATP activity.
authors.affiliation: Department of Medical Physiology and Sports Medicine, JJRC, Utrecht, Netherlands.
authors.forename: E L
authors.initials: EL
authors.lastname: De Beer
authors.forename: H
authors.initials: H
authors.lastname: Finkle
authors.forename: E E
authors.initials: EE
authors.lastname: Voest
authors.forename: B G
authors.initials: BG
authors.lastname: Van Heijst
authors.forename: P
authors.initials: P
authors.lastname: Schiereck
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D003497:Cyclic N-Oxides / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology*; D005609:Free Radicals; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000187:drug effects*; D011817:Rabbits
chemical_list: D003497:Cyclic N-Oxides; D005609:Free Radicals; D004317:Doxorubicin; D002118:Calcium; C003959:TEMPO