title: Enteric GABA: mode of action and role in the regulation of the peristaltic reflex.
abstract: The mode of action of gamma-aminobutyric acid (GABA) and the role of myenteric GABA neurons in the regulation of peristalsis were examined in various preparations of rat colonic muscle. GABA had no contractile, relaxant, or modulatory effect on smooth muscle cells isolated from the circular muscle layer. In innervated circular muscle strips, GABA elicited concentration-dependent relaxation accompanied by release of vasoactive intestinal peptide (VIP). Relaxation and VIP release were inhibited by tetrodotoxin and by the GABAA receptor antagonist bicuculline but not by the GABAB receptor antagonist phaclofen. Relaxation was inhibited by the VIP receptor antagonist VIP-(10-28) implying that VIP release was coupled to muscle relaxation. Relaxation was augmented by atropine implying that GABA also activated cholinergic neurons causing release of acetylcholine that attenuated the relaxant response. This pharmacological profile was evident when GABA was released from intrinsic GABA neurons during peristalsis induced by radial stretch. Blockade of GABAA receptors with bicuculline inhibited the descending relaxation mediated by VIP motor neurons and the ascending contraction mediated by cholinergic motor neurons. Stimulation of these receptors with exogenous GABA had the opposite effect. We conclude that on release from myenteric neurons, GABA acts via GABAA receptors on cholinergic and VIP motor neurons responsible for the two components of the peristaltic reflex.
authors.affiliation: Department of Physiology, Medical College of Virginia, Richmond 23298-0711.
authors.forename: J R
authors.initials: JR
authors.lastname: Grider
authors.forename: G M
authors.initials: GM
authors.lastname: Makhlouf
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001640:Bicuculline / Q000494:pharmacology; D002469:Cell Separation; D003106:Colon / Q000166:cytology / Q000187:drug effects; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects; D010528:Peristalsis / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D012018:Reflex / Q000502:physiology*; D014660:Vasoactive Intestinal Peptide / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology / Q000502:physiology*
chemical_list: D014660:Vasoactive Intestinal Peptide; D005680:gamma-Aminobutyric Acid; D001285:Atropine; D001640:Bicuculline