Detection of gastrin and its messenger RNA in Zollinger-Ellison tumors by non-radioactive in situ hybridization and immunocytochemistry.
Gastrin immunocytochemistry and non-radioactive in situ hybridization, using biotinylated oligonucleotide probes, for gastrin mRNA have been used for studying a retrospective material of six gastrin-producing (Zollinger-Ellison) tumors. Hybridization results for gastrin mRNA were positive in all six, while gastrin immunoreactivity could be detected in five tumors. In one of the patients, different areas of the same tumor displayed differences in immunoreactivity to gastrin, but were uniformly hybridization positive. Weak hybridization signals were detected in liver metastases from a necropsy case, while the gastrin immunostaining was more pronounced. The results show that non-radioactive hybridization methods are applicable to routine clinical specimens stored for as long as 16 years and that in situ hybridization may be a useful complement to immunocytochemical diagnosis, particularly in cases where high synthesis and little storage of hormonal products occur.
Larsson
L I
LI
Department of Molecular Cell Biology, State Serum Institute, Copenhagen, Denmark.
Hougaard
D M
DM
D001483:Base Sequence; D005753:Gastric Mucosa / Q000737:chemistry*; D015408:Gastrinoma / Q000737:chemistry* / Q000235:genetics; D005755:Gastrins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010190:Pancreatic Neoplasms / Q000737:chemistry* / Q000235:genetics; D011706:Pyloric Antrum; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D015043:Zollinger-Ellison Syndrome / Q000235:genetics / Q000378:metabolism*
D005755:Gastrins; D012333:RNA, Messenger