title: Rapid high-performance liquid chromatographic determination of urinary N-(1-methylethyl)-N'-phenyl-1,4-benzenediamine in workers exposed to aromatic amines.
abstract: A procedure has been developed for determining N-(1-methylethyl)-N'-phenyl-1,4-benzenediamine in urine by using high-performance liquid chromatography. The method uses chloroform extraction for partial clean-up of the urine sample. The separation is carried out on a reversed-phase column using 65 mmol/l aqueous ammonium acetate in acetonitrile (30:70, v/v) as the mobile phase. The column effluent is monitored at 290 nm with an ultraviolet detector. The analyte is separated from other normal urine constituents in less than 4 min. Peak height and concentration are linearly related. Coefficients of variation assessed for within-day reproducibility were 5.9 and 3.7% at concentrations of 22.3 and 92.1 micrograms/l, respectively. The mean analytical recovery from urine samples spiked with known amounts of amine was 89.7 +/- 6.8%. The request of only a small volume of urine and the simple pre-treatment procedure makes it suitable for the routine monitoring of the exposure of rubber vulcanization workers to aromatic amines.
authors.affiliation: Central Analysis Laboratory, Ospedale Mauriziano, Turin, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Pellegrino
authors.forename: M
authors.initials: M
authors.lastname: Petrarulo
authors.forename: E
authors.initials: E
authors.lastname: Testa
authors.forename: A
authors.initials: A
authors.lastname: Nicolotti
mesh_terms: D000588:Amines / Q000009:adverse effects*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods; D006801:Humans; D009784:Occupational Diseases / Q000139:chemically induced*; D010655:Phenylenediamines / Q000652:urine*
chemical_list: D000588:Amines; D010655:Phenylenediamines; C014627:N-isopropyl-N-phenyl-4-phenylenediamine