A comparison of some simplified lactobionate preservation solutions with standard UW solution and Eurocollins solution for pancreas preservation.
Fifty-two rat pancreas transplants were performed to investigate which components of the UW solution were essential for successful pancreas preservation. LEW rats were used and the pancreata stored at 4 degrees C for 48 hr after flushing with commercial UW solution (ViaSpan, DuPont Pharmaceuticals) or a number of simplified solutions. Following storage the pancreata were transplanted into syngeneic recipient animals with streptozotocin-induced diabetes mellitus. Graft function was assessed by regular postoperative blood sugar measurements and a glucose tolerance test on the 14th postoperative day. With commercial UW solution, 4 of 9 recipients (44%) showed satisfactory graft function, while only one of 5 pancreata preserved using Eurocollins solution demonstrated satisfactory function. With solution A, in which hydroxyethyl starch and insulin were omitted from the standard UW solution, 3 of 7 recipients (43%) showed satisfactory function. Omission of glutathione, allopurinol, and adenosine from this solution (solution B) gave satisfactory function in 4 of 8 cases (50%). Substitution of raffinose in solution B with an equimolar concentration of glucose (solution C) resulted in acceptable function in 5 of 8 cases (62%). Increasing the raffinose concentration in solution B to 100 mM/L resulted in only 2 of 8 grafts (25%) with adequate function. By contrast, reversing the Na/K concentrations in solution A resulted in 100% (7/7) satisfactory graft function. We conclude that the rat pancreas can be successfully transplanted following 48-hr cold preservation using UW solution and some simplified versions, and that a substantially simplified lactobionate-based solution with a reversed sodium/potassium ratio improved survival.
Urushihara
T
T
Second Department of Surgery, Hiroshima University School of Medicine, Japan.
Sumimoto
R
R
Sumimoto
K
K
Jamieson
N V
NV
Ito
H
H
Ikeda
M
M
Fukuda
Y
Y
Dohi
K
K
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004187:Disaccharides / Q000494:pharmacology*; D005978:Glutathione; D007328:Insulin; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D016035:Pancreas Transplantation; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D012996:Solutions
D004187:Disaccharides; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; C005608:lactobionic acid; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose