[Effects of lidocaine on stimulation-coupled responses of neutrophils and protein kinase C activity].
The effects of lidocaine on superoxide generation and other stimulation-coupled responses of neutrophils induced by 12-phorbol myristate 13-acetate (PMA) were studied. Depolarization of membrane potential, superoxide generation and chemiluminescence response were inhibited by lidocaine in a concentration dependent manner. Lidocaine inhibited protein kinase C (PKC) activity in a manner competitive with phosphatidylserine. Lidocaine also inhibited the phosphorylation of 47 kDa neutrophil cytoplasmic protein, a phosphorylated protein required for activation of NADPH oxidase. The inhibitory action of lidocaine on PKC activity may correlate with the inhibition of superoxide generation induced by PMA.
Tomoda
M
M
Department of Anesthesiology and Resuscitology, Kochi Medical School, Nankoku.
Ueda
W
W
Tsuchiya
M
M
Hirakawa
M
M
D000818:Animals; D003864:Depression, Chemical; D006168:Guinea Pigs; D008012:Lidocaine / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D011493:Protein Kinase C / Q000187:drug effects / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D008012:Lidocaine; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate