Role of amyloid precursor protein (APP): study with antisense transfection of human neuroblastoma cells.
The function of amyloid precursor protein (APP) was investigated in human neuroblastoma La-N-1 cells by stable transfection with a DNA construct encoding antisense APP mRNA. Levels of APP mRNA, as well as proteins, were reduced by 80-90% in antisense APP transfected (ASAT) cells. ASAT cells exhibited three main features as a result of APP gene expression deprivation: (1) a 30% reduction in cell proliferation, (2) reduced cell adhesion that could be reversed by the addition of La-N-1 conditioned media as a source of secreted APP, and (3) a two- and four-fold increase in neurite-bearing cells suggesting that cellular APP may be involved in neurite extension. The first two features confirm previously reported functions for APP in proliferation and adhesion of non-neuronal cell types but the use of neuroblastoma cells in this study disclose a novel role for cellular APP in neurite extension.
LeBlanc
A C
AC
Department of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
Kovacs
D M
DM
Chen
H Y
HY
Villaré
F
F
Tykocinski
M
M
Autilio-Gambetti
L
L
Gambetti
P
P
D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000502:physiology*; D015153:Blotting, Western; D002455:Cell Division; D002460:Cell Line; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D006801:Humans; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D009447:Neuroblastoma; D016372:RNA, Antisense / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D015183:Restriction Mapping; D014162:Transfection
D016564:Amyloid beta-Protein Precursor; D016372:RNA, Antisense; D012333:RNA, Messenger