The effect of serum obtained before and after treatment for endometriosis on in vitro development of two-cell mouse embryos.
OBJECTIVE
To evaluate the effect of serum obtained before and after treatment for endometriosis on in vitro development of two-cell mouse embryos.


METHODS
Pretreatment and post-treatment comparison of mouse embryo development in serum supplements from patients with endometriosis; results were compared using chi 2 analysis.


METHODS
Infertility Clinic, Shimane Medical University, Izumo, Japan.


METHODS
Ten consecutive women with endometriosis who underwent treatment for the disease. Seven women who were managed expectantly served as controls.


METHODS
All patients underwent laparoscopic or conservative surgery. This was followed by a 6-month course of either danazol 400 mg/d (6 patients) or buserelin acetate 900 micrograms/d (4 patients).


METHODS
Total number of embryos that reached blastocyst stage after 72 hours of incubation were compared before and after treatment.


RESULTS
Before treatment, 23% of the embryos reached blastocyst stage, whereas 8 of the 10 serum samples were embryo toxic. After treatment, significantly more embryos (59%) developed to blastocysts, and only one serum sample remained embryo toxic. In the control group, there was no significant difference in the number of embryos that reached blastocyst stage after expectant treatment.


CONCLUSIONS
The embryo toxicity of serum samples from patients with endometriosis is lost after treatment.
Abu-Musa
A
A
Department of Obstetrics and Gynecology, Shimane Medical University, Izumo, Japan.
Takahashi
K
K
Kitao
M
M
D000818:Animals; D046508:Culture Techniques; D005314:Embryonic and Fetal Development; D004715:Endometriosis / Q000097:blood* / Q000628:therapy; D005260:Female; D006801:Humans; D051379:Mice / Q000196:embryology; D008813:Mice, Inbred ICR