A viral protease-mediated cleavage of the transmembrane glycoprotein of Mason-Pfizer monkey virus can be suppressed by mutations within the matrix protein.
The envelope glycoprotein precursor of retroviruses undergoes proteolytic cleavage in the Golgi complex to yield the mature surface and transmembrane (TM) glycoproteins of the virus. We report here that the TM glycoprotein of Mason-Pfizer monkey virus undergoes a second proteolytic processing event during a late maturation step that can follow virus release and Gag polyprotein cleavage. Cleavage results in the conversion of the cell-associated TM glycoprotein (gp22) to a virus-associated gp20. Processing continues after virus release and yields virions that contain predominantly gp20. A mutation within the active site of the Mason-Pfizer monkey virus aspartyl protease was shown to block both TM glycoprotein cleavage and the processing of the Gag polyprotein precursor. The role of the viral protease in cleavage of the TM glycoprotein localizes the cleavage site to the cytoplasmic domain of this protein. Surprisingly, point mutations within the matrix (MA) coding region of the gag gene can affect the extent to which gp22 is processed to gp20 and in one case [p10(MA)-A79V] results in greater than 90% inhibition of gp22 cleavage. The data provide genetic evidence of a specific interaction between the capsid proteins and the cytoplasmic domain of the TM glycoprotein of a retrovirus. This interaction is required for cytoplasmic domain cleavage to occur and may play a critical role in virus assembly and viral infectivity.
Brody
B A
BA
Department of Microbiology, University of Alabama, Birmingham 35294.
Rhee
S S
SS
Sommerfelt
M A
MA
Hunter
E
E
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D010450:Endopeptidases / Q000378:metabolism*; D015683:Gene Products, gag / Q000096:biosynthesis / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D007930:Leucine / Q000378:metabolism; D016093:Mason-Pfizer monkey virus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D013447:Sulfites / Q000494:pharmacology; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*
D015683:Gene Products, gag; D008562:Membrane Glycoproteins; D013447:Sulfites; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins; D010450:Endopeptidases; D007930:Leucine; C009279:sodium bisulfite