title: Involvement of chloride in renin secretion from isolated rat glomeruli.
abstract: The sensitivity of renin release to changes in anion and calcium concentrations was assessed in superfused rat glomeruli with attached juxtaglomerular cells. Isosmotic substitution of Cl-with gluconate (1/12, 1/6, 1/3, 2/3, or total exchange), isethionate (15 or 101 mM), or sulfate (10 mM) inhibited renin release reversibly. Substitution of Cl- with nitrate (101 mM) stimulated renin secretion. Substitution with iodide (15 or 101 mM) had no consistent effect. The stimulation induced by calcium-free solutions was high in May and low in September. In the absence of chloride, the response to calcium-free solution was inhibited similarly all year. In May reintroduction of calcium and chloride stimulated renin release, suggesting that releasable renin had been stockpiled during the exposure to calcium-free solution. In September reintroduction of calcium and chloride inhibited renin release. It is concluded that the renin secretory process has a demand for permeant anions. The stimulation caused by low external calcium involves at least two mechanisms: one is anion sensitive, powerful, varies with the season, and includes a recruitment phenomenon; another is anion insensitive and weak.
authors.affiliation: University Institute of Experimental Medicine, Panum Institute, Copenhagen Denmark.
authors.forename: O
authors.initials: O
authors.lastname: Skøtt
authors.forename: B L
authors.initials: BL
authors.lastname: Jensen
mesh_terms: D000818:Animals; D000838:Anions; D002118:Calcium / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology / Q000502:physiology*; D005942:Gluconates / Q000494:pharmacology; D066298:In Vitro Techniques; D007454:Iodides / Q000494:pharmacology; D007513:Isethionic Acid / Q000494:pharmacology; D007606:Juxtaglomerular Apparatus / Q000201:enzymology; D007678:Kidney Glomerulus / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D007700:Kinetics; D008297:Male; D008954:Models, Biological; D009566:Nitrates / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000378:metabolism*; D012621:Seasons
chemical_list: D000838:Anions; D002712:Chlorides; D005942:Gluconates; D007454:Iodides; D009566:Nitrates; D007513:Isethionic Acid; D012083:Renin; D002118:Calcium