A simple technique for postembedding ultrastructural immunogold labeling.
A simple and reproducible method for postembedding ultrastructural immunogold labeling is described. Tissues are fixed in Carson-Millonig phosphate-buffered 4% formaldehyde, and then are embedded in LR White resin. Immunogold labeling of ultrathin sections is accomplished by means of an indirect method that employs a nonconjugated primary antibody and gold particles conjugated to antibody to immunoglobulin G. Technical aspects of fixation, embedding, and immunolabeling that influence the success of this protocol are discussed.
Lockard
V G
VG
Department of Pathology, University of Mississippi Medical Center, Jackson 39216.
D000906:Antibodies / Q000276:immunology; D006046:Gold; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods*; D008722:Methods; D016253:Microscopy, Immunoelectron / Q000379:methods*
D000906:Antibodies; D007074:Immunoglobulin G; D006046:Gold