Distribution patterns and frequency of proliferating cells in cutaneous keratinocytic neoplasms. Immunohistochemical study with a monoclonal antibody (TOB7) used against proliferating cell nuclear antigen.
BACKGROUND
Almost all markers for proliferating cells need freshly frozen tissues for evaluation; therefore retrospective study is impossible.


OBJECTIVE
In the present study, a murine monoclonal antibody (TOB7) against the proliferating cell nuclear antigen (PCNA) was used for the analysis of cell kinetics of cutaneous keratinocytic neoplasms. The antibody is applicable to formalin-fixed, paraffin-embedded tissues.


METHODS
The frequency of PCNA-positive cells and their distribution patterns were immunohistochemically investigated in various cutaneous keratinocytic neoplasms.


RESULTS
Squamous cell carcinoma and Bowen's disease showed significantly increased numbers of PCNA-positive cells when compared with other keratinocytic neoplasms. A characteristic marginal or random distribution pattern of PCNA-positive cells was observed in the lesions of each disease category.


CONCLUSIONS
Important information on the growth dynamics of keratinocytic neoplasms was obtained in this retrospective immunohistochemical study.
Saida
T
T
Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan.
Dohi
S
S
Sadaki
M
M
Tokuda
Y
Y
Ikegawa
S
S
Takasaki
Y
Y
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology*; D002455:Cell Division; D002467:Cell Nucleus / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology*; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000453:epidemiology / Q000378:metabolism / Q000473:pathology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor