title: Biochemical, genetic and molecular characterization of new respiratory-deficient mutants in Chlamydomonas reinhardtii.
abstract: Eight respiratory-deficient mutants of Chlamydomonas reinhardtii have been isolated after mutagenic treatment with acriflavine or ethidium bromide. They are characterized by their inability to grow or their very reduced growth under heterotrophic conditions. One mutation (Class III) is of nuclear origin whereas the seven remaining mutants (Classes I and II) display a predominantly paternal mt- inheritance, typical of mutations residing in the mitochondrial DNA. Biochemical analysis has shown that all mutants are deficient in the cyanide-sensitive cytochrome pathway of the respiration whereas the alternative pathway is still functional. Measurements of complexes II + III (antimycin-sensitive succinate-cytochrome c oxido-reductase) and complex IV (cytochrome c oxidase) activities allowed to conclude that six mutations have to be localized in the mitochondrial apocytochrome b (COB) gene, one in the mitochondrial cytochrome oxidase subunit I (COI) gene and one in a nuclear gene encoding a component of the cytochrome oxidase complex. By using specific probes, we have moreover demonstrated that five mutants (Class II mutants) contain mitochondrial DNA molecules deleted in the terminal end containing the COB gene and the telomeric region; they also possess dimeric molecules resulting from end-to-end junctions of deleted monomers. The two other mitochondrial mutants (Class I) have no detectable gross alteration. Class I and Class II mutants can also be distinguished by the pattern of transmission of the mutation in crosses. An in vivo staining test has been developed to identify rapidly the mutants impaired in cyanide-sensitive respiration.
authors.affiliation: Département de Botanique, Université de Liège, Belgium.
authors.forename: M P
authors.initials: MP
authors.lastname: Dorthu
authors.forename: S
authors.initials: S
authors.lastname: Remy
authors.forename: M R
authors.initials: MR
authors.lastname: Michel-Wolwertz
authors.forename: L
authors.initials: L
authors.lastname: Colleaux
authors.forename: D
authors.initials: D
authors.lastname: Breyer
authors.forename: M C
authors.initials: MC
authors.lastname: Beckers
authors.forename: S
authors.initials: S
authors.lastname: Englebert
authors.forename: C
authors.initials: C
authors.lastname: Duyckaerts
authors.forename: F E
authors.initials: FE
authors.lastname: Sluse
authors.forename: R F
authors.initials: RF
authors.lastname: Matagne
mesh_terms: D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives / Q000494:pharmacology; D001059:Apoproteins / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D016825:Chlamydomonas reinhardtii / Q000201:enzymology / Q000235:genetics*; D003124:Colorimetry; D003573:Cytochrome b Group / Q000235:genetics* / Q000378:metabolism; D045303:Cytochromes b; D004272:DNA, Mitochondrial; D009154:Mutation; D010101:Oxygen Consumption / Q000235:genetics*; D010641:Phenotype; D015183:Restriction Mapping; D013778:Tetrazolium Salts
chemical_list: D001059:Apoproteins; D003573:Cytochrome b Group; D004272:DNA, Mitochondrial; D013778:Tetrazolium Salts; C032456:antimycin; D000968:Antimycin A; C009591:triphenyltetrazolium; D045303:Cytochromes b