Complete primary structure of bovine beta 2-glycoprotein I: localization of the disulfide bridges.
The complete primary structure of bovine beta 2-glycoprotein I was determined by a combination of cDNA and peptide sequencing. Bovine beta 2-glycoprotein I was purified from citrated plasma, and by sequencing selected peptides, the complete disulfide bridge patterns of the 11 disulfide bridges were established as well as the positions of the five asparagine-linked carbohydrate groups. Bovine beta 2-glycoprotein I comprises five mutually homologous domains or Short Consensus Repeats, each containing two disulfide bridges, except for the fifth most C-terminal domain which diverges from the Short Consensus Repeat consensus by containing an additional disulfide bridge. In the four N-terminal domains, the first and third and the second and fourth half-cystines are disulfide-linked, while in the fifth domain the first and fourth, the second and fifth, and the third and sixth half-cystines are disulfide-linked.
Bendixen
E
E
Department of Molecular Biology and Plant Physiology, University of Aarhus, Denmark.
Halkier
T
T
Magnusson
S
S
Sottrup-Jensen
L
L
Kristensen
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004220:Disulfides / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011487:Protein Conformation; D012091:Repetitive Sequences, Nucleic Acid; D053482:beta 2-Glycoprotein I
D004220:Disulfides; D006023:Glycoproteins; D053482:beta 2-Glycoprotein I; D004247:DNA