High-sensitivity laser-induced fluorescence detection of native proteins in capillary electrophoresis.
A highly sensitive laser-induced (LIF) detection scheme for native, tryptophan- or tyrosine-containing proteins in capillary electrophoresis (CE) has been demonstrated. The 275.4 nm line from an argon-ion laser is used to excite native protein fluorescence. A limit of detection (LOD) (S/N = 2) of 1 x 10(-10) M for conalbumin represents a 140-fold improvement over earlier reports. With stacking at injection, the LOD is 3 x 10(-12) M. Linear dynamic ranges of at least 5 and 4 orders of magnitude for, respectively, tryptophan and bovine serum albumin are found. The practical performance and blueprint of an easily constructed, rugged, compact and user-friendly LIF detector for CE are shown.
Lee
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Department of Chemistry, Iowa State University, Ames 50011.
Yeung
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ES
D004586:Electrophoresis / Q000379:methods*; D007834:Lasers; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
D011506:Proteins; D014443:Tyrosine; D014364:Tryptophan