Role of endogenous platelet-activating factor in caerulein-induced acute pancreatitis in rats: protective effects of a PAF-antagonist.
The role of endogenous platelet-activating factor (PAF) in the pathogenesis of acute pancreatitis was investigated by determining whether CV-6209, a selective PAF antagonist, confers protection against caerulein-induced acute pancreatitis in rats. Continuous intravenous infusion of caerulein (5 micrograms/kg x h) induced time-dependent increase in serum pancreatic enzymes, pancreatic weight, and protein content of the pancreas, and produced histologic evidence of acute pancreatitis. Pretreatment with CV-6209 (1 mg/kg) significantly inhibited the elevation of serum pancreatic enzymes, pancreatic weight, and protein content of the pancreas. Caerulein-induced tissue oedema and recruitment of leucocytic cells were markedly ameliorated with CV-6209. Platelet-activating factor may be released endogenously and may play a role during acute pancreatitis.
Fujimura
K
K
Third Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Kubota
Y
Y
Ogura
M
M
Yamaguchi
T
T
Binnaka
T
T
Tani
K
K
Kitagawa
S
S
Mizuno
T
T
Inoue
K
K
D000208:Acute Disease; D000818:Animals; D002108:Ceruletide; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000201:enzymology / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors* / Q000502:physiology*; D011726:Pyridinium Compounds / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D010972:Platelet Activating Factor; D011726:Pyridinium Compounds; C053321:CV 6209; D002108:Ceruletide