Physical linkage of the A-raf-1, properdin, synapsin I, and TIMP genes on the human and mouse X chromosomes.
Genes encoding the neuron-specific phosphoprotein synapsin I (SYN1), the glycoprotein tissue inhibitor of metalloproteinases (TIMP), the proto-oncogene A-raf-1 (ARAF1), and properdin (PFC), a positive regulator of the alternative pathway of human complement, lie within a conserved synteny encompassing the proximal short arm of the human X chromosome (Xp21.1-p11) and the centromeric end of the mouse X chromosome (A1-A5). We have used a mouse interspecific cross to demonstrate genetic linkage of Syn-1, Timp, and Araf and also show physical linkage, with Timp lying only 10 kb from Araf, within an intron of the Syn-1 gene. Detailed restriction mapping shows that Timp is transcribed in the same direction as Araf but in the opposite direction to the Syn-1 gene. Analysis of the corresponding region of the human X chromosome indicates a similar arrangement and in addition shows that the properdin gene lies within 5 kb of the 5' end of the synapsin I gene.
Derry
J M
JM
MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, United Kingdom.
Barnard
P J
PJ
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors; D051379:Mice; D008969:Molecular Sequence Data; D011414:Properdin / Q000235:genetics; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D013045:Species Specificity; D016704:Synapsins / Q000235:genetics*; D019714:Tissue Inhibitor of Metalloproteinases; D014960:X Chromosome
D006023:Glycoproteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016704:Synapsins; D019714:Tissue Inhibitor of Metalloproteinases; D011414:Properdin; D004247:DNA; D008666:Metalloendopeptidases