title: Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase. Sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti.
abstract: cDNA clones encoding the fatty-acid- biosynthetic enzyme NADPH-linked 3-oxoacyl-(acyl carrier protein) (ACP) reductase were isolated from a Brassica napus (rape) developing seed library and from an Arabidopsis thaliana (thale cress) leaf library. The N-terminal end of the coding region shows features typical of a stromal-targeting plastid-transit peptide. The deduced amino acid sequences have 41% and 55% identity respectively with the nodG-gene product of Rhizobium meliloti, one of the host-specific genes that restrict infectivity of this bacterium to a small range of host plants. The probability that the nodG-gene product is a oxoreductase strengthens the hypothesis that some of the host-specific nod-gene products are enzymes which synthesize polyketides that uniquely modify the Rhizobium nodulation signal molecule.
authors.affiliation: School of Biological Sciences, University of Durham, U.K.
authors.forename: A R
authors.initials: AR
authors.lastname: Slabas
authors.forename: D
authors.initials: D
authors.lastname: Chase
authors.forename: I
authors.initials: I
authors.lastname: Nishida
authors.forename: N
authors.initials: N
authors.lastname: Murata
authors.forename: C
authors.initials: C
authors.lastname: Sidebottom
authors.forename: R
authors.initials: R
authors.lastname: Safford
authors.forename: P S
authors.initials: PS
authors.lastname: Sheldon
authors.forename: R G
authors.initials: RG
authors.lastname: Kekwick
authors.forename: D G
authors.initials: DG
authors.lastname: Hardie
authors.forename: R W
authors.initials: RW
authors.lastname: Mackintosh
mesh_terms: D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D015723:Gene Library; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D016962:Sinorhizobium meliloti / Q000235:genetics*
chemical_list: D000429:Alcohol Oxidoreductases; D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase