title: Cytoplasmic microtubule assembly is altered in cytoplasts.
abstract: Nucleation of microtubule (MT) organization of the cytoplasmic microtubule complex (CMTC) from the microtubule organizing centres (MTOC) was studied in enucleated cytoplasts of human diploid fibroblast (MRC-5) and mouse peritoneal macrophages in culture. Cytoplasts of both cell types could not organize the complete CMTC. Aberrant MT patterns were seen in MRC-5 cells while mouse macrophages showed occurrence of few short MT. The studies suggest that nucleus may have a role in determining CMTC.
authors.affiliation: Cancer Research Institute, Tata Memorial Centre, Parel, India.
authors.forename: S
authors.initials: S
authors.lastname: Guha Roy
authors.forename: A N
authors.initials: AN
authors.lastname: Bhisey
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000648:ultrastructure; D003571:Cytochalasin B / Q000494:pharmacology*; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000648:ultrastructure; D051379:Mice; D008866:Microsurgery; D008870:Microtubules / Q000737:chemistry / Q000187:drug effects*; D015739:Nocodazole / Q000494:pharmacology*
chemical_list: D003571:Cytochalasin B; D015739:Nocodazole