Dual fluorescent in situ hybridisation for simultaneous detection of X and Y chromosome-specific probes for the sexing of human preimplantation embryonic nuclei.
Dual fluorescent in situ hybridisation has been used for the simultaneous detection of X and Y chromosome-specific probes in single cleavage nuclei from disaggregated 4- to 7-cell human embryos. Based on the presence of a Y signal or 2 X signals in the absence of a Y, 89% of poor quality metaphases and 72% of interphase nuclei could be classified as male or female. With further refinements, this technique will offer a credible alternative to the polymerase chain reaction for the diagnosis of sex in human preimplantation embryos in families segregating for X-linked genetic disease.
Griffin
D K
DK
Department of Genetics and Biometry, University College London, UK.
Wilton
L J
LJ
Handyside
A H
AH
Winston
R M
RM
Delhanty
J D
JD
D001757:Blastomeres; D015342:DNA Probes / Q000235:genetics; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012732:Sex Determination Analysis; D014960:X Chromosome; D014998:Y Chromosome
D015342:DNA Probes