Polar localization of a bacterial chemoreceptor.
The bacterial chemotaxis signal transducer MCP is an integral membrane receptor protein. The chemoreceptor is localized at the flagellum-bearing pole of Caulobacter crescentus swarmer cells. Amino-terminal sequences of the MCP target the protein to the membrane while the carboxy-terminal portion of the protein is responsible for polar localization. The C. crescentus and Escherichia coli MCPs have highly conserved carboxy-terminal domains, and when an E. coli MCP is expressed in C. crescentus, it is targeted to the swarmer cell progeny. These results suggest that subcellular localization of a prokaryotic protein involves interaction of specific regions of the protein with unique cell sites that contain either localized binding proteins or a specific secretory apparatus.
Alley
M R
MR
Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, California 94305-5427.
Maddock
J R
JR
Shapiro
L
L
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D016935:Caulobacter crescentus / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure*; D002630:Chemotactic Factors / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D002633:Chemotaxis / Q000235:genetics; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D000072236:Methyl-Accepting Chemotaxis Proteins; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D011233:Precipitin Tests; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics
D001426:Bacterial Proteins; D002630:Chemotactic Factors; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D011993:Recombinant Fusion Proteins