In situ localization of transforming growth factor beta and S-phase cells in patients with acute myeloid leukemia and myelodysplastic syndrome.
In situ localization of transforming growth factor beta (TGF-B) was accomplished in 16 acute myeloid leukemia (AML) and 11 myelodysplastic syndrome (MDS) cases. A modified and greatly shortened procedure for immunohistochemical localization of TGF-B using a monoclonal antibody was undertaken in plastic embedded bone marrow biopsy sections that demonstrated excellent preservation of morphologic detail. In addition, a double-label procedure was developed for the simultaneous detection of TGF-B and cells actively engaged in DNA-synthesis which were labeled in vivo by infusing thymidine analogues. Marked variation in intensity of TGF-B staining in interstitial areas of biopsies was noted among different patients. TGF-B was also conspicuously present in the basement membrane of blood vessel endothelial cells and the cytoplasm of megakaryocytes. Double-labeling revealed that S-phase cells in a number of MDS cases appeared clustered together and that megakaryocytes in S-phase were completely devoid of TGF-B.
Qadir
K
K
Department of Internal Medicine, University of Cincinnati, Ohio 45267-0508.
Bokhari
S A
SA
Miller
M A
MA
Siegrist
C
C
Bismayer
J
J
Raza
A
A
D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism / Q000473:pathology*; D009190:Myelodysplastic Syndromes / Q000378:metabolism / Q000473:pathology*; D016196:S Phase; D016212:Transforming Growth Factor beta / Q000032:analysis*
D016212:Transforming Growth Factor beta