Use of the thermostable glucose-6-phosphate dehydrogenase as a label enzyme in steroid enzyme immunoassays.
A thermostable glucose-6-phosphate dehydrogenase was assessed as a label enzyme in a testosterone enzyme immunoassay system. Enzyme labeling of a steroid with the enzyme was carried out by the N-succinimidyl ester method. A dose-response curve with a satisfactory sensitivity could be obtained by the use of the enzyme-labeled antigen. This enzyme should be useful in hapten enzyme immunoassays because of its relatively high stability.
Hosoda
H
H
Pharmaceutical Institute, Tohoku University, Sendai, Japan.
Fukuda
K
K
Takahashi
N
N
Goto
J
J
D005954:Glucosephosphate Dehydrogenase / Q000032:analysis*; D007124:Immunoenzyme Techniques; D013256:Steroids / Q000032:analysis / Q000378:metabolism*
D013256:Steroids; D005954:Glucosephosphate Dehydrogenase