[Effect of serum factors on the differentiation of a colon cancer cell line Caco-2].
A human colon cancer cell line Caco-2 undergoes spontaneous enterocytic differentiation under the standard culture condition. We examined the changes in the proliferative activity of Caco-2 cells during the differentiation, and the effect of serum factors on this spontaneous differentiation. Caco-2 cells cultured in FCS-containing media exhibited the differentiation phenotypes such as dome formation and increase of ALP activity after the 6th day of culture. Cell cycle analysis showed a marked decrease in S phase cell population during the cell differentiation. When Caco-2 cells were cultured in FCS free media, the cell population of S phase remained unchanged during culture period and the differentiation phenotypes were not induced. The addition of FCS to Caco-2 cell cultured in the absence of FCS resulted in the rapid induction of the differentiation phenotypes. These results suggest that differentiation of Caco-2 cells is closely related to the reduction of cell proliferation and requires some factors in FCS.
Hara
A
A
Department of Internal Medicine, School of Medicine, Keio University.
Hibi
T
T
Yoshioka
M
M
Toda
K
K
Watanabe
N
N
Morita
M
M
Suzuki
T
T
Serizawa
H
H
Saito
H
H
Iwao
Y
Y
D000818:Animals; D001685:Biological Factors / Q000097:blood / Q000494:pharmacology*; D002417:Cattle; D002454:Cell Differentiation; D002455:Cell Division; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D014407:Tumor Cells, Cultured / Q000473:pathology
D001685:Biological Factors