Peroxisomal protein import. In vivo evidence for a novel translocation competent compartment.
In homogeneous of isolated hepatocytes separated by Nycodenz density gradient centrifugation, two peroxisomal populations are identified that differ in buoyant density. Organelles present in a high density fraction (1.22-1.23 g/cm3) represent mature peroxisomes. Vesicles of intermediate density (1.16-1.17 g/cm3) represent mature peroxisomes. Vesicles of intermediate density (1.16-1.17 g/cm3) are present in much lower concentration and seem to play a particular role in the import and distribution of newly synthesized peroxisomal proteins. In a typical pulse-chase experiment with a 7.5 min pulse, peroxisomal acyl-CoA oxidase is first imported into the peroxisomal fraction of intermediate density. After a chase of up to 30 min, the enzyme is found in mature peroxisomes.
Heinemann
P
P
Institut für Biochemie I, Universität Heidelberg, Germany.
Just
W W
WW
D042962:Acyl-CoA Oxidase; D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008830:Microbodies / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D008565:Membrane Proteins; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase