title: Quinalphos-induced suppression of spermatogenesis, plasma gonadotrophins, testicular testosterone production, and secretion in adult rats.
abstract: Quinalphos (O,O-diethyl-O-[quinoxalinyl-(2)-thionophosphate]) is a well-known organophosphorus insecticide used extensively in agriculture that adversely interferes with the activity of testicular steroidogenic enzymes in rats. To investigate its effects on spermatogenesis, the other function of testes, quantitative evaluation of different varieties of germ cells at stage VII of the seminiferous epithelium cycle, namely, type A spermatogonia (ASg), preleptotene spermatocytes (pLSc), midpachytene spermatcytes (mPSc), and step 7 spermatids (7Sd), along with the radioimmunoassay of plasma FSH, LH, testosterone, and testicular testosterone, were performed in Wistar rats following treatment with quinalphos (250 micrograms/kg, ip) for approximately one (13 days) and two cycles (26 days) of the seminiferous epithilium. Massive degeneration of all varieties of germ cells at stage VII, remarkable reduction in the sperm count, and significant reductions in plasma concentrations of FSH and testosterone, along with testicular testosterone, were observed after quinalphos treatment. Significant reduction in the plasma concentration of LH was observed only after treatment for two cycles. Administration of human chorionic gonadotrophin for 26 days in rats injected with quinalphos partially prevented the degeneration of germ cells and increased testosterone production. It is suggested that quinalphos may have a suppressive influence on gonadotrophin release but its direct detrimental action at the level of the testes may also be responsible for the observed changes in spermatogenesis and in testicular testosterone production in rats.
authors.affiliation: Department of Physiology, University College of Science and Technology, Calcutta, India.
authors.forename: A
authors.initials: A
authors.lastname: Ray
authors.forename: S
authors.initials: S
authors.lastname: Chatterjee
authors.forename: S
authors.initials: S
authors.lastname: Ghosh
authors.forename: K
authors.initials: K
authors.lastname: Bhattacharya
authors.forename: A
authors.initials: A
authors.lastname: Pakrashi
authors.forename: C
authors.initials: C
authors.lastname: Deb
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000097:blood; D006062:Gonadotropins / Q000097:blood*; D007306:Insecticides / Q000633:toxicity*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D013076:Sperm Count / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000032:analysis / Q000097:blood / Q000378:metabolism
chemical_list: D006063:Chorionic Gonadotropin; D006062:Gonadotropins; D007306:Insecticides; D009946:Organothiophosphorus Compounds; C009145:quinalphos; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone