The Azotobacter vinelandii recA gene: sequence analysis and regulation of expression.
The nucleotide (nt) sequence of the Azotobacter vinelandii recA gene (Av-recA) was determined and compared with the recA sequences from Pseudomonas aeruginosa (Pa-recA), a soil bacterium, and Escherichia coli (Ec-recA), an enteric bacterium. The Av-recA gene and the deduced aa sequence were found to be more similar to their Pa-recA counterparts than to the Ec-recA gene and protein. Expression of Av-recA was found to be autoregulatory. Unlike Ec-recA and Pa-recA, however, expression of Av-recA was weakly enhanced upon DNA damage. In E. coli, expression of an Av-recA::lacZ fusion was poor, but its autoregulation was similar to that of Ec-recA. Av-recA expression, however, could not induce the repair system response in E. coli.
Venkatesh
T V
TV
Genetic Engineering Unit, Jawaharlal Nehru University, New Delhi, India.
Das
H K
HK
D000595:Amino Acid Sequence; D016948:Azotobacter vinelandii / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000528:radiation effects; D007700:Kinetics; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000235:genetics; D011938:Rec A Recombinases / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000528:radiation effects; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000528:radiation effects
D011993:Recombinant Fusion Proteins; D011938:Rec A Recombinases; D001616:beta-Galactosidase