Inhibition of iron overload toxicity in rat hepatocyte cultures by pyoverdin Pf, the siderophore of Pseudomonas fluorescens.
The effect of the pyoverdin Pf (an iron chelating agent isolated and purified from Pseudomonas fluorescens CCM 2798) was studied on iron overloaded rat hepatocyte cultures. Iron overload was obtained by addition of 5-80 microM ferric nitrilotriacetate to the culture medium. Twenty-four hours after iron treatment, a significant increase in aspartate aminotransferase and lactate dehydrogenase in the culture medium was observed. This corresponded to intracellular decrease in the activity of these two enzymes and correlated with a decrease in albumin secretion and an increase in total free malondialdehyde production. The iron toxicity was inhibited by desferrioxamine B. Pyoverdin Pf added to the hepatocyte cultures served as an effective agent to prevent iron toxicity induced in overload. The observed effect of the pyoverdin Pf was as potent as that of desferrioxamine B.
Jego
P
P
Liver Research Unit, INSERM U49, Rennes, France.
Hubert
N
N
Morel
I
I
Pasdeloup
N
N
Ocaktan
A
A
Abdallah
M
M
Brissot
P
P
Lescoat
G
G
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D003676:Deferoxamine / Q000494:pharmacology; D005290:Ferric Compounds / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007501:Iron / Q000633:toxicity*; D007502:Iron Chelating Agents / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects*; D008969:Molecular Sequence Data; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D009842:Oligopeptides; D010860:Pigments, Biological / Q000737:chemistry / Q000494:pharmacology*; D011551:Pseudomonas fluorescens; D051381:Rats; D011919:Rats, Inbred Strains
D005290:Ferric Compounds; D007502:Iron Chelating Agents; D009842:Oligopeptides; D010860:Pigments, Biological; C042453:pyoverdin; D007501:Iron; D003676:Deferoxamine; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate