Ultramicrodetermination of cyanocobalamin in elemental diet by solid-phase extraction and high-performance liquid chromatography with visible detection.
The ultramicrodetermination of cyanocobalamin (9 ng/g) in elemental diet containing 46 kinds of compounds, with concentrations at least 50-10(6) times higher than that of cyanocobalamin, was performed by Sep-Pak C(18) purification and concentration of cyanocobalamin followed by HPLC with detection at 550 nm. The method is simple, rapid, sensitive and reproducible. The calibration graph was linear in the range of 0-0.2 micrograms. The recovery of cyanocobalamin was over 95% by the standard addition method. There was good agreement between the cyanocobalamin concentrations indicated and found.
Iwase
H
H
D002851:Chromatography, High Pressure Liquid; D004032:Diet; D006801:Humans; D013057:Spectrum Analysis; D014805:Vitamin B 12 / Q000032:analysis*
D014805:Vitamin B 12