title: Evolutionary relationship of NAD(+)-dependent D-lactate dehydrogenase: comparison of primary structure of 2-hydroxy acid dehydrogenases.
abstract: A comparison of the primary structures of NAD(+)-dependent D-lactate dehydrogenase with L-lactate dehydrogenase and L-malate dehydrogenase failed to show any sequence similarity. However, D-2-hydroxyisocaproate dehydrogenase from Lactobacillus casei, glycerate dehydrogenase from cucumber, D-3-phosphoglycerate dehydrogenase and erythronate 4-phosphate dehydrogenase from Escherichia coli showed 38%, 24%, 24% and 22% amino acid identity, respectively. The profile analysis of the aligned sequences confirmed their relatedness. The hydropathy profiles of the aligned dehydrogenases were almost identical between residues 100-300 indicating largely preserved folding patterns of their polypeptide chains. The data suggest that L- and D-specific 2-hydroxy acid dehydrogenase genes evolved from two different ancestors and thus represent two different sets of enzyme families.
authors.affiliation: Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Kochhar
authors.forename: P E
authors.initials: PE
authors.lastname: Hunziker
authors.forename: P
authors.initials: P
authors.lastname: Leong-Morgenthaler
authors.forename: H
authors.initials: H
authors.lastname: Hottinger
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D005075:Biological Evolution; D002237:Carbohydrate Dehydrogenases / Q000235:genetics*; D016208:Databases, Factual; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D007780:Lacticaseibacillus casei / Q000235:genetics; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D050543:Phosphoglycerate Dehydrogenase; D010944:Plants / Q000201:enzymology; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D012984:Software
chemical_list: D029968:Escherichia coli Proteins; D009243:NAD; D000429:Alcohol Oxidoreductases; D002237:Carbohydrate Dehydrogenases; C047961:2-hydroxyisocaproate dehydrogenase; C061651:pdxB protein, E coli; D007770:L-Lactate Dehydrogenase; C020669:Glycerate dehydrogenase; D050543:Phosphoglycerate Dehydrogenase