Characterization of the low density lipoprotein receptor activity in buffalo rat liver (BRL-3A) cells.
1. BRL-3A cells possess a specific LDL receptor with an apparent mol. wt of 160,000 that binds, with saturation, both human and rat 125I-LDL. 2. Like human fibroblasts, BRL-3A cells also bind, internalize and degrade 125I-hLDL but to a lesser extent. 3. BRL-3A cells also bind the monoclonal antibody against rat liver LDL receptor P1B3. Moreover the LDL receptor activity increases when cells are preincubated with medium containing 5% of LPDS. 4. As with human (h) fibroblasts, treatment of BRL-3A cells with 10(-7) M insulin enhances binding (30%), internalization (18%) and degradation (20%) of 125I-hLDL.
Santillo
M
M
Istituto di Scienze Fisiologiche Umane, Università di Napoli, Italia.
Mondola
P
P
Santangelo
F
F
Belfiore
A
A
Tedesco
I
I
Laezza
C
C
Cerbone
R
R
Romano
A
A
Bifulco
M
M
D000818:Animals; D002460:Cell Line; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009115:Muridae; D011973:Receptors, LDL / Q000378:metabolism*
D011973:Receptors, LDL