Carcinogen-DNA adducts in human alveolar macrophages.
The occurrence of 7 beta,8 alpha-dihydroxy-(9 alpha,10 alpha)-epoxy-7,8,9,10- tetrahydrobenzo(a)pyrene (BPDE-)-DNA adducts was investigated by synchronous fluorescence spectrophotometry in human pulmonary alveolar macrophages (PAM) collected by bronchoalveolar lavage. In preliminary assays BPDE-DNA adducts were consistently detected in PAM of smokers, whereas samples from nonsmokers did not exhibit specific peaks.
Izzotti
A
A
Institute of Hygiene and Preventive Medicine, University of Genoa, Italy.
Bagnasco
M
M
Rossi
G A
GA
De Flora
S
S
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis*; D000328:Adult; D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000378:metabolism; D004247:DNA / Q000032:analysis*; D018736:DNA Adducts; D005260:Female; D006801:Humans; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012140:Respiratory Tract Diseases / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D018736:DNA Adducts; C043931:benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D004247:DNA