title: Electrophoretic separation of two Aedes caspius Pallas forms from Egypt.
abstract: Isozyme banding patterns of the recently recognized autogenous stenogamous and anautogenous eurygamous sexually isolated Aedes caspius forms were investigated using horizontal starch gel electrophoresis of 5 enzymes. The esterase assay clearly separated in 2 forms, a faster band being characteristic of autogenous individuals. Other tested enzymes were encoded by 5 loci that were highly polymorphic. The "malic" enzyme locus was unambiguously diagnostic, specific, non-overlapping alleles at that locus were revealed in each form. Other enzyme loci screened exhibited at least 2 alleles common to both forms, allelic frequency differences at least loci could not separate the 2 forms except at the cathodal MDH-1 locus (malate dehydrogenase assay). Probability of correct diagnosis at the MDH-1 locus was as high as 93% so that it may be used as a confirmatory test.
authors.affiliation: Ain Shams Research and Training Center on Vectors of Diseases, Ain Shams University, Egypt.
authors.forename: A M
authors.initials: AM
authors.lastname: Gad
authors.forename: M M
authors.initials: MM
authors.lastname: Hassan
authors.forename: L S
authors.initials: LS
authors.lastname: el-Sherif
authors.forename: H A
authors.initials: HA
authors.lastname: Farid
mesh_terms: D000330:Aedes / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D000483:Alleles; D000818:Animals; D004534:Egypt; D004592:Electrophoresis, Starch Gel; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics
chemical_list: D007527:Isoenzymes