title: Effects of pressure on glucose transport in human erythrocytes.
abstract: The operation of the human red cell glucose transporter has been studied at normal and high hydrostatic pressure to identify the step(s) which involve a volume change. Pressure inhibited zero-trans and equilibrium exchange influx to similar extents, by decreasing the Vmax but not significantly changing the Km. The Bmax and Kd of specific [3H]cytochalasin B binding were unaffected by pressure indicating no change to the number or affinity of functional transporters at pressure. Passive glucose transport was inhibited by pressure in a manner consistent with permeation across the lipid bilayer. These data indicate that there is a major change in volume during the translocation step of the glucose transporter which is rate-limiting for transport.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Manchester, UK.
authors.forename: S D
authors.initials: SD
authors.lastname: Thorne
authors.forename: A C
authors.initials: AC
authors.lastname: Hall
authors.forename: A G
authors.initials: AG
authors.lastname: Lowe
mesh_terms: D001692:Biological Transport; D003571:Cytochalasin B / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009997:Osmotic Pressure
chemical_list: D009004:Monosaccharide Transport Proteins; D003571:Cytochalasin B; D005947:Glucose