title: [Potential application of DTPA for improving the image quality in radioimmunoscintigraphy with 111In labeled monoclonal antibodies].
abstract: Studies on application of diethylenetriamine-pentaacetic acid (DTPA) to decrease the non-specific localization of 111In-labeled antibodies (111In-MoAb) to the liver were performed. The transchelation of 111In to the molecule of M.W. 70,000-90,000 by the incubation of 111In-MoAb in human serum was inhibited by adding DTPA. These in vitro findings were also demonstrated by serum analysis after 111In-MoAb injection to the normal mice and postinjection of DTPA. By addition of DTPA, the uptake of 111In-MoAb by isolated rat hepatocytes was inhibited, but the 111In-release was unaffected. The addition of DTPA had no effect on uptake of 111In-MoAb and 111In-release in tumor cells. These results suggest that DTPA administration following 111In-MoAb injection may inhibit transchelation of 111In to transferrin or other serum proteins, so decrease non-specific liver localization of 111In and give rapid blood clearance.
authors.affiliation: Department of Radiology, Ehime University School of Medicine.
authors.forename: K
authors.initials: K
authors.lastname: Ochi
authors.forename: T
authors.initials: T
authors.lastname: Fujii
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: A
authors.initials: A
authors.lastname: Akamune
authors.forename: K
authors.initials: K
authors.lastname: Hamamoto
mesh_terms: D000818:Animals; D007205:Indium Radioisotopes / Q000378:metabolism; D008099:Liver / Q000000981:diagnostic imaging* / Q000378:metabolism; D008297:Male; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism; D004369:Pentetic Acid / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D016719:Radioimmunodetection; D051381:Rats; D011919:Rats, Inbred Strains; D014168:Transferrin / Q000378:metabolism
chemical_list: D007205:Indium Radioisotopes; D014168:Transferrin; D004369:Pentetic Acid