title: Identification of o-hydroxyphenylacetaldehyde as a major metabolite of coumarin in rat hepatic microsomes.
abstract: The metabolism of [3-14C]coumarin has been studied in hepatic microsomes from control (corn-oil treated) and Aroclor 1254-treated (100 mg/kg body weight/day, 5 days, ip) rats. [3-14C]Coumarin metabolites in incubate extracts were separated by HPLC and identified by comparison with the retention times of known coumarin metabolites. The major product produced by incubation of 0.25-2.5 mM-[3-14C]coumarin with both control and Aroclor 1254-induced hepatic microsomes was a novel coumarin metabolite. This novel metabolite was extracted from pooled microsomal incubations, purified by semi-preparative HPLC and identified by mass spectrometry as o-hydroxyphenylacetaldehyde (o-HPA). Some possible pathways for the formation of o-HPA from coumarin are proposed.
authors.affiliation: BIBRA Toxicology International, Carshalton, Surrey, UK.
authors.forename: B G
authors.initials: BG
authors.lastname: Lake
authors.forename: D J
authors.initials: DJ
authors.lastname: Osborne
authors.forename: D G
authors.initials: DG
authors.lastname: Walters
authors.forename: R J
authors.initials: RJ
authors.lastname: Price
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D020111:Chlorodiphenyl (54% Chlorine); D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001140:Aroclors; D003374:Coumarins; D020111:Chlorodiphenyl (54% Chlorine); D009249:NADP; C070647:2-hydroxyphenylacetaldehyde; C030123:coumarin; D000079:Acetaldehyde