Radioimmunoscintigraphic approach for the in vivo detection of tuberculomas--a preliminary study in a rabbit model.
Radioimmunoscintigraphic approach could provide a viable non-invasive alternative for the diagnosis of deeply situated tuberculomas. We have evaluated this in a rabbit model constructed to give a characteristic localized tubercular lesion, with complimentary morphological, histological and antigenic profiles. 131I-anti Myobacterium bovis (BCG) antibody was shown to localize at the lesion, where as 131I-bovine serum albumin and 99mTc-red blood cell scans were negative. The clearance of intradermally-injected tuberculous antigen could be traced into ascending lymph nodes using 131I-anti M. bovis (BCG) antibody.
Malpani
B L
BL
Radiation Medicine Center, BARC, Tata Memorial Center Annexe, Parel, Bombay, India.
Kadival
G V
GV
Samuel
A M
AM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D004912:Erythrocytes / Q000378:metabolism; D007150:Immunohistochemistry; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011817:Rabbits; D016719:Radioimmunodetection; D012711:Serum Albumin, Radio-Iodinated; D014375:Tuberculoma / Q000000981:diagnostic imaging* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D012711:Serum Albumin, Radio-Iodinated