title: Determination of the enantiomeric composition of samples of cocaine by normal-phase high-performance liquid chromatography with UV detection.
abstract: A high-performance liquid chromatographic method has been developed for the quantitation of the enantiomers of cocaine. Mixtures of the naturally occurring (-)-cocaine and synthetically produced (+)-cocaine were hydrolyzed in water to (+) and (-)-benzoyl ecgonine. Esterification with an optically pure 2-octanol resulted in diastereoisomers that could be separated on bare silica gel using an acetonitrile-aqueous ammonium phosphate mobile phase.
authors.affiliation: Department of Chemistry, Brookhaven National Laboratory, Upton, NY 11973.
authors.forename: R R
authors.initials: RR
authors.lastname: MacGregor
authors.forename: J S
authors.initials: JS
authors.lastname: Fowler
authors.forename: A P
authors.initials: AP
authors.lastname: Wolf
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: C005618:benzoylecgonine; D003042:Cocaine