Identification of a tolfenamic acid metabolite in the horse by gas chromatography-mass spectrometry.
A tolfenamic acid metabolite, a hydroxylated product, has been identified in equine plasma and urine samples using gas chromatography-mass spectrometry in the electron-impact and chemical-ionization modes. The method also allows the qualitative monitoring of the elimination of the drug and its metabolites from plasma. The two compounds are detected up to 48 and 24 h, respectively, after a single oral administration of a 30 mg/kg dose. The simultaneous detection of the two products increases the reliability of anti-doping control analysis.
Jaussaud
P
P
INRA-ENVL Research Laboratory for Sport Equine, National School of Veterinary Medicine, Marcy l'Etoile, France.
Guieu
D
D
Courtot
D
D
Barbier
B
B
Bonnaire
Y
Y
D000818:Animals; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry; D006736:Horses / Q000097:blood*; D062367:ortho-Aminobenzoates / Q000097:blood* / Q000652:urine
D062367:ortho-Aminobenzoates; C009500:tolfenamic acid