title: Detection of calbindin-D immunoreactivity in human follicular dendritic cells.
abstract: The calcium-binding protein calbindin-D (28 kD) has been analysed immunohistochemically in different human lymphoid tissues. Combined immunohistochemical staining showed that calbindin-D (28 kD) is expressed by only a proportion of dendritic cells within the light zone of germinal centres, where antigens in the form of immune complexes are trapped and presented to B lymphocytes. All other cell types including macrophages, interdigitating cells, and various lymphocyte populations were negative. The expression of calbindin-D in this functionally relevant subset of follicular dendritic cells could have a role in the regulation of proliferation and selection of memory B-cells by modulating the concentration of calcium ions. Calbindin-D may be a useful marker for analysing in situ the phases of follicular development in different physiological and pathological conditions.
authors.affiliation: Istituto di Anatomia Patologica, Università di Verona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Chilosi
authors.forename: C
authors.initials: C
authors.lastname: Doglioni
authors.forename: P
authors.initials: P
authors.lastname: Pelosio
authors.forename: A
authors.initials: A
authors.lastname: Mombello
authors.forename: L
authors.initials: L
authors.lastname: Montagna
authors.forename: A
authors.initials: A
authors.lastname: Benedetti
authors.forename: M
authors.initials: M
authors.lastname: Lestani
authors.forename: P
authors.initials: P
authors.lastname: Giovanni
authors.forename: F
authors.initials: F
authors.lastname: Menestrina
mesh_terms: D064026:Calbindins; D003713:Dendritic Cells / Q000737:chemistry*; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D012507:Sarcoidosis / Q000378:metabolism; D014123:Toxoplasmosis / Q000378:metabolism
chemical_list: D064026:Calbindins; D064030:S100 Calcium Binding Protein G