title: Developmental regulation of Sertoli cell aromatase activity and plasminogen activator production by hormones, retinoids and the testicular paracrine factor, PModS.
abstract: Testicular peritubular cells produce a paracrine factor termed PModS that has dramatic effects on Sertoli cell function in vitro. The current study was designed to examine the actions of PModS and hormones on Sertoli cell aromatase activity and plasminogen activator production at various stages of pubertal development. Sertoli cells were isolated from 10-, 20-, and 35-day-old rats (ages correspond to prepubertal, midpubertal, and late-pubertal stages of development). Aromatase activity was found to be high and hormone-responsive in prepubertal Sertoli cells and to decline and be nonresponsive to hormones in late-pubertal Sertoli cells. FSH was the only hormone found to influence aromatase activity and estrogen production. PModS alone was not found to affect aromatase activity at any of the developmental stages examined. Interestingly, PModS was found to suppress the ability of FSH to stimulate aromatase activity and estrogen production in midpubertal Sertoli cells. Results imply that PModS may promote Sertoli cell differentiation to a more adult stage of development that is less responsive to FSH in stimulating aromatase activity. In contrast to aromatase activity, plasminogen activator production was found to increase during pubertal development. Production of Sertoli cell tissue-type plasminogen activator (tPa) was stimulated by FSH at each of the developmental stages examined, whereas production of urokinase-type plasminogen activator (uPa) was influenced by FSH only in prepubertal Sertoli cells. Insulin also stimulated uPa and tPa production by prepubertal Sertoli cells, and retinol significantly suppressed uPa production and the ability of FSH to stimulate tPa production by midpubertal Sertoli cells.
authors.affiliation: Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-6600.
authors.forename: M
authors.initials: M
authors.lastname: Rosselli
authors.forename: M K
authors.initials: MK
authors.lastname: Skinner
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D008297:Male; D010960:Plasminogen Activators / Q000378:metabolism*; D051381:Rats; D012176:Retinoids / Q000494:pharmacology*; D012708:Sertoli Cells / Q000201:enzymology* / Q000378:metabolism; D013735:Testicular Hormones / Q000494:pharmacology*
chemical_list: C054951:P-Mod-S paracrine factor; D012176:Retinoids; D013735:Testicular Hormones; D005640:Follicle Stimulating Hormone; D001141:Aromatase; D010960:Plasminogen Activators