title: Characterization of two galactosidases extracted from wheat germ with a hydroalcoholic solvent.
abstract: Alpha- and beta-D-galactosidases were characterized from a hydroalcoholic extract of wheat germ (Triticum vulgare). Kinetic constants (Vmax and KM) and the optimal pHs for the hydrolysis of p-nitrophenyl galactopyranosides by both enzymes were determined. These enzymes presented a high stability in hydroalcoholic medium and were inhibited by iodoacetamide and sodium p-hydroxy-mercuribenzoate.
authors.affiliation: Département de Biochimie des Glycoconjugués et des Lectines Endogènes, CNRS, Orléans, France.
authors.forename: M P
authors.initials: MP
authors.lastname: Papet
authors.forename: D
authors.initials: D
authors.lastname: Delay
authors.forename: M
authors.initials: M
authors.lastname: Monsigny
authors.forename: F
authors.initials: F
authors.lastname: Delmotte
mesh_terms: D000085:Acetates / Q000737:chemistry; D019342:Acetic Acid; D000431:Ethanol / Q000737:chemistry; D005696:Galactosidases / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014908:Triticum
chemical_list: D000085:Acetates; D000431:Ethanol; D005696:Galactosidases; D019342:Acetic Acid; C018824:ammonium acetate