Incorporation of N-acyl-2-amino-2-deoxy-hexoses into glycosphingolipids of the pheochromocytoma cell line PC 12.
The synthesis of N-acyl-2-amino-2-deoxy-hexoses, their metabolism and their incorporation into glycosphingolipids of rat pheochromocytoma cell line PC 12 were investigated. The data indicate that in PC 12 cells the N-acyl-2-amino-2-deoxy-hexoses, N-propanoyl-D-glucosamine and N-butanoyl-D-glucosamine are metabolized to the corresponding phosphates, and that N-propanoyl-D-glucosamine is also metabolized to N-propanoyl neuraminic acid. Using variously radiolabelled N-acyl-2-amino-2-deoxy-hexoses, their incorporation into glycosphingolipids was shown.
Kayser
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H
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany.
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Paul
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Zeitler
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Reutter
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D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006028:Glycosphingolipids / Q000378:metabolism*; D006601:Hexoses / Q000096:biosynthesis / Q000378:metabolism*; D016716:PC12 Cells
D006028:Glycosphingolipids; D006601:Hexoses