New Granada Medium for detection and identification of group B streptococci.
A methotrexate-containing medium for the detection of beta-hemolytic group B streptococci from clinical specimens on the basis of detection of pigment is described. The medium contained peptone, starch, serum, MgSO4, glucose, pyruvate, methotrexate (as pigment enhancer), phosphate-morpholine-propanesulfonic acid buffer, and selective agents. The recovery of beta-hemolytic group B streptococci was comparable to that obtained with selective broth.
de la Rosa
M
M
Microbiology Service, Virgen de las Nieves Hospital, Granada, Spain.
Perez
M
M
Carazo
C
C
Pareja
L
L
Peis
J I
JI
Hernandez
F
F
D001431:Bacteriological Techniques; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D008727:Methotrexate; D010858:Pigmentation; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D008727:Methotrexate