title: Phenotypic and functional analysis of lamina propria mononuclear cells from colonoscopic biopsy specimens in patients with ulcerative colitis.
abstract: Phenotypic and functional analysis was performed with lamina propria mononuclear cells (LPMCs) isolated from colonoscopic biopsies in 27 patients with ulcerative colitis (UC). The proportion of T lymphocytes displaying HLA-DR antigens, interleukin 2 (IL-2) receptor, and transferrin receptor was greater in active UC than in control diseases. When LPMCs were cultured with IL-2 or phytohemagglutinin for 72 h, there were no significant differences in the proportion of cells bearing these activation markers between active UC and controls. The proportion of CD56+ cells and lymphokine-activated killer (LAK) cell activity was lower in LPMCs from active UC than in control cells, and depletion of CD56+ cells from control lamina propria cells essentially eliminated LAK cell activity. Mucosal T lymphocytes may be activated in vivo during active inflammation in UC, and lower levels of intestinal LAK cell activity may be related to the decrease of CD56+ cells under these conditions.
authors.affiliation: First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Haruta
authors.forename: K
authors.initials: K
authors.lastname: Kusugami
authors.forename: A
authors.initials: A
authors.lastname: Kuroiwa
authors.forename: K
authors.initials: K
authors.lastname: Ina
authors.forename: M
authors.initials: M
authors.lastname: Shinoda
authors.forename: K
authors.initials: K
authors.lastname: Morise
authors.forename: H
authors.initials: H
authors.lastname: Iokawa
authors.forename: M
authors.initials: M
authors.lastname: Morita
authors.forename: A
authors.initials: A
authors.lastname: Ishihara
authors.forename: S
authors.initials: S
authors.lastname: Sarai
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D000911:Antibodies, Monoclonal; D001706:Biopsy; D002469:Cell Separation; D003093:Colitis, Ulcerative / Q000276:immunology* / Q000473:pathology*; D003113:Colonoscopy; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology*; D015979:Killer Cells, Lymphokine-Activated / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000502:physiology*; D008297:Male; D008875:Middle Aged
chemical_list: D000911:Antibodies, Monoclonal