Induction of the 72-kD heat shock protein in organ-cultured normal human skin.
To study the induction of heat shock protein (HSP) of normal human skin, the indirect immunofluorescence method, using monoclonal antibody directed against 72-kD HSP, was applied in organ-cultured normal human skin that was treated with heat, UV, or chemicals. The present study provided new evidence that HSP 72 was induced not only by heat and chemical agents, such as L-azetidine 2-carboxylic acid, and sodium arsenite, but also by ultraviolet (UV B and C). The result suggests that normal human skin has an induced protective function against numerous environmental stresses.
Muramatsu
T
T
Department of Dermatology, Nara Medical University, Japan.
Tada
H
H
Kobayashi
N
N
Yamji
M
M
Shirai
T
T
Ohnishi
T
T
D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D001383:Azetidinecarboxylic Acid / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D006801:Humans; D009924:Organ Culture Techniques; D012867:Skin / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D017670:Sodium Compounds; D014466:Ultraviolet Rays
D018053:Arsenites; D006360:Heat-Shock Proteins; D017670:Sodium Compounds; C017947:sodium arsenite; D001383:Azetidinecarboxylic Acid; D001151:Arsenic