Crystallization of the B800-820 light-harvesting complex from Rhodopseudomonas acidophila strain 7750.
The B800-820 light-harvesting complex, an integral membrane protein, from Rhodopseudomonas acidophila strain 7750 has been crystallized. The tabular plates have a hexagonal unit cell of a = b = 121.8 A and c = 283.1 A and belong to the space group R32. X-ray diffraction data have been collected to 6 A resolution, using an area detector on a rotating anode source. The B800-820 light-harvesting complex is comprised of four low molecular weight apoproteins (B800-820 alpha 1, B800-820 alpha 2, B800-820 beta 1 and B800-820 beta 2). Polyacrylamide gel electrophoresis shows that the complex exists as an oligomeric assembly, with an apparent molecular weight of 92,000.
Guthrie
N
N
Department of Chemistry, University of Glasgow, Scotland.
MacDermott
G
G
Cogdell
R J
RJ
Freer
A A
AA
Isaacs
N W
NW
Hawthornthwaite
A M
AM
Halloren
E
E
Lindsay
J G
JG
D003460:Crystallization; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D012241:Rhodopseudomonas / Q000737:chemistry*; D014961:X-Ray Diffraction
D045322:Photosynthetic Reaction Center Complex Proteins