title: Characterization and detoxification of an easily prepared acellular pertussis vaccine. Antigenic role of the A protomer of pertussis toxin.
abstract: An acellular pertussis vaccine was prepared from pertussis vaccine concentrates by eliminating the cells by centrifugation and subsequent acid precipitation. SDS-PAGE analysis indicated the presence of 17 clearly defined bands including the subunits of pertussis toxin. The acellular preparation was detoxified with glutaraldehyde and lost its undesirable effects (leukocytosis-promoting activity, histamine-sensitizing activity and decrease in weight gain) but retained a very good protective activity (PD50 approximately 2 micrograms protein/mouse). Sera from mice immunized with this preparation indicated an antibody response against the A protomer of pertussis toxin.
authors.affiliation: Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, DF.
authors.forename: J A
authors.initials: JA
authors.lastname: García-Sáinz
authors.forename: M T
authors.initials: MT
authors.lastname: Romero-Avila
authors.forename: A
authors.initials: A
authors.lastname: Ruíz-Arriaga
authors.forename: J
authors.initials: J
authors.lastname: Ruíz-Puente
authors.forename: C
authors.initials: C
authors.lastname: Agundis
authors.forename: V
authors.initials: V
authors.lastname: Ortiz
authors.forename: A
authors.initials: A
authors.lastname: Isibasi
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D005976:Glutaral / Q000494:pharmacology; D051379:Mice; D010567:Pertussis Vaccine / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D010567:Pertussis Vaccine; D005976:Glutaral