title: The potential clinical impact of the fine needle aspiration/flow cytometric diagnosis of malignant lymphoma.
abstract: The objective of this study was to determine the potential application of fine needle aspiration (FNA) and flow cytometry (FCM) for the diagnosis of non-Hodgkin's lymphoma (NHL). At our institution, we have used FNA coupled with FCM to accurately diagnose both reactive lymphoid processes and most lymphomas in a prospective series of more than 90 patients. A retrospective review of 266 patients with NHL who were treated at the London (Ont.) Regional Cancer Centre over a five-year period was performed. Two-thirds presented with palpable lymphadenopathy which would have been amenable to FNA. Based upon the results of our prospective study, FNA/FCM could have provided an accurate diagnosis of malignancy for these patients. Furthermore, the subclassification of the lymphomas would have been accurate in 75% of cases. For 25% of patients, including those with T-cell lymphoma, accurate subclassification may have been problematic. Fifty of the 266 patients developed recurrences during the period of study. Ninety per cent of these were at sites amenable to FNA. Furthermore, the cytomorphology could have indicated whether transformation to a higher grade of lymphoma had occurred. Currently, FNA coupled with FCM is an under-utilized diagnostic technique which has great potential for the investigation of patients with both newly diagnosed and recurrent lymphoma.
authors.affiliation: Department of Otolaryngology, University of Western Ontario, London, Canada.
authors.forename: W G
authors.initials: WG
authors.lastname: Chernoff
authors.forename: H B
authors.initials: HB
authors.lastname: Lampe
authors.forename: H
authors.initials: H
authors.lastname: Cramer
authors.forename: D
authors.initials: D
authors.lastname: Banerjee
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D002648:Child; D003936:Diagnosis, Computer-Assisted; D005069:Evaluation Studies as Topic; D005260:Female; D005434:Flow Cytometry / Q000379:methods / Q000592:standards*; D006782:Hospitals, Special; D006801:Humans; D015994:Incidence; D008207:Lymphatic Metastasis; D016131:Lymphocyte Subsets; D008228:Lymphoma, Non-Hodgkin / Q000145:classification / Q000453:epidemiology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis / Q000453:epidemiology / Q000473:pathology; D009367:Neoplasm Staging; D009864:Ontario / Q000453:epidemiology; D011446:Prospective Studies; D012189:Retrospective Studies; D012680:Sensitivity and Specificity