A rapid and simple quantification of human apolipoprotein E-rich high-density lipoproteins in serum.
A rapid and simple method for the quantitative determination of human serum apo E-rich high-density lipoproteins is described. A sample was divided into two parts; one part was mixed with an equal volume of 13% polyethylene glycol 6000, and the other part was mixed with a solution containing dextran sulfate, sodium phosphotungstate, and Mg2+, respectively. The mixed solutions were centrifuged (2000 g; 15 min). The supernate obtained by the former procedure contained both apo E-rich HDL and apo E-poor HDL, but that obtained by the latter procedure contained solely apo E-poor HDL. The serum apo E-rich HDL concentration in terms of apo E (E) and cholesterol (C), was given by the following equations: E = EP x 2, and C = (CP - CD) x 2, where EP and CP were the concentrations of apo E and cholesterol, respectively, in the supernate obtained with 13% polyethylene glycol, and CD was the concentration of cholesterol in the supernate obtained with the mixture solution of dextran sulfate, sodium phosphotungstate, and Mg2+. Normal serum apo E-rich HDL concentrations were 2.6 +/- 1.5 and 6.7 +/- 2.3 mg/dl (means +/- SD, n = 38) in terms of apo E and cholesterol, respectively. Apo E-rich HDL was increased strikingly in the sera from three patients with hepatobiliary diseases.
Chiba
H
H
Department of Laboratory Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Akizawa
K
K
Fujisawa
S
S
Osaka-Nakamori
T
T
Iwasaki
N
N
Suzuki
H
H
Intoh
S
S
Matsuno
K
K
Mitamura
T
T
Kobayashi
K
K
D000328:Adult; D001057:Apolipoproteins E / Q000097:blood*; D011232:Chemical Precipitation; D002908:Chronic Disease; D016264:Dextran Sulfate; D005260:Female; D006505:Hepatitis / Q000097:blood*; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008105:Liver Cirrhosis, Biliary / Q000097:blood*; D008274:Magnesium; D008875:Middle Aged; D010772:Phosphotungstic Acid; D011092:Polyethylene Glycols
D001057:Apolipoproteins E; D008075:Lipoproteins, HDL; D010772:Phosphotungstic Acid; D011092:Polyethylene Glycols; D016264:Dextran Sulfate; D008274:Magnesium