Effects of tautomycin, a protein phosphatase inhibitor, on recycling of mammalian cell surface molecules.
The effects of tautomycin, a protein phosphatase inhibitor, on recycling of cell surface molecules were studied with transferrin receptor (TFR) of human myeloid leukemia K562 cells and with CD4 of murine thymocytes. Tautomycin increased expression of TFR of K562 cells whereas phorbol dibutylate (PDBu) decreased it. Tautomycin inhibited PDBu-induced down-regulation of CD4 although it did not induce up-regulation. Okadaic acid also inhibited down-regulation of CD4 which was induced by PDBu. The results suggest that certain inhibitors of protein phosphatases preferentially inhibit endocytosis of cell surface molecules.
Kurisaki
T
T
Department of Agricultural Chemistry, University of Tokyo, Japan.
Magae
J
J
Isono
K
K
Nagai
K
K
Yamasaki
M
M
D000470:Alkaloids / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D015704:CD4 Antigens / Q000378:metabolism*; D002227:Carbazoles; D015536:Down-Regulation / Q000187:drug effects*; D004988:Ethers, Cyclic / Q000494:pharmacology; D026121:Indole Alkaloids; D019319:Okadaic Acid / Q000031:analogs & derivatives; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011714:Pyrans / Q000494:pharmacology; D011990:Receptors, Transferrin / Q000378:metabolism*; D013141:Spiro Compounds; D019311:Staurosporine
D000470:Alkaloids; D000935:Antifungal Agents; D015704:CD4 Antigens; D002227:Carbazoles; D004988:Ethers, Cyclic; D026121:Indole Alkaloids; D011714:Pyrans; D011990:Receptors, Transferrin; D013141:Spiro Compounds; C053079:tautomycin; D019319:Okadaic Acid; D015240:Phorbol 12,13-Dibutyrate; C051904:dinophysistoxin 1; C049985:staurosporine aglycone; D011493:Protein Kinase C; D019311:Staurosporine