The myelopoietic effects of a Serratia marcescens-derived biologic response modifier in a mouse model of thermal injury.
The proliferative defects observed in phagocytic stem cells after major thermal injuries may be caused by an inadequate production of colony-stimulating factors (CSFs), a family of hemopoietic cytokines necessary for the production and function of granulocytes and monocytes. In this study a biologic response modifier (S-BRM) consisting of sized vesicles derived from the cell membrane and ribosomes of Serratia marcescens was investigated in a mouse model of thermal injury to determine its ability to augment postburn myelopoiesis. Treatment of burned mice with S-BRM was well tolerated and was associated with statistically significant increases in absolute numbers of circulating granulocytes and monocytes compared with burned mice receiving saline solution. In addition, the size of the splenic myeloid stem cell compartment, as measured by granulocyte-macrophage stem cell colony formation in soft agar, was markedly expanded. Finally, plasma levels of CSF were increased significantly in burned mice receiving S-BRM but were not elevated in burned littermates treated with saline solution. These data suggest that production of CSF is suboptimal after thermal injury and S-BRM is capable of up-regulating postburn myelopoiesis by causing the release of CSF into the systemic circulation.
Peterson
V M
VM
Department of Surgery, University of Colorado Health Sciences Center, Denver 80262.
Rundus
C H
CH
Reinoehl
P J
PJ
Schroeter
S R
SR
McCall
C A
CA
Bartle
E J
EJ
D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D002056:Burns / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D002462:Cell Membrane; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000134:cerebrospinal fluid; D005260:Female; D006098:Granulocytes / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D007155:Immunologic Factors; D008264:Macrophages / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012270:Ribosomes; D012706:Serratia marcescens
D003115:Colony-Stimulating Factors; D007155:Immunologic Factors