Recruitment of coat proteins onto Golgi membranes in intact and permeabilized cells: effects of brefeldin A and G protein activators.
Brefeldin A (BFA) causes a rapid redistribution of coat proteins (e.g., gamma-adaptin) associated with the clathrin-coated vesicles that bud from the trans-Golgi network (TGN), while the clathrin-coated vesicles that bud from the plasma membrane are unaffected. gamma-Adaptin redistributes with the same kinetics as beta-COP, a coat protein associated with the non-clathrin-coated vesicles that bud from the Golgi complex. Upon removal of BFA, however, gamma-adaptin recovers its perinuclear distribution more rapidly. Redistribution of both proteins can be prevented by pretreating cells with AlF4-. Recruitment of adaptors from the cytosol onto the TGN membrane has been reconstituted in a permeabilized cell system and is increased by addition of GTP gamma S and blocked by addition of BFA. These results suggest a role for G proteins in the control of the clathrin-coated vesicle cycle at the TGN and further extend the similarities between clathrin-coated vesicles and non-clathrin-coated vesicles.
Robinson
M S
MS
Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, England.
Kreis
T E
TE
D033965:Adaptor Protein Complex alpha Subunits; D033982:Adaptor Protein Complex gamma Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000535:Aluminum / Q000494:pharmacology; D017607:Aluminum Compounds; D015153:Blotting, Western; D020126:Brefeldin A; D002460:Cell Line; D020756:Coatomer Protein; D003517:Cyclopentanes / Q000494:pharmacology*; D005459:Fluorides; D005461:Fluorine / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000502:physiology; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000031:analogs & derivatives / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*
D033965:Adaptor Protein Complex alpha Subunits; D033982:Adaptor Protein Complex gamma Subunits; D033942:Adaptor Proteins, Vesicular Transport; D017607:Aluminum Compounds; D020756:Coatomer Protein; D003517:Cyclopentanes; D008869:Microtubule-Associated Proteins; D011506:Proteins; C034882:guanosine 5'-O-(1-thiotriphosphate); D020126:Brefeldin A; C050992:tetrafluoroaluminate; D005461:Fluorine; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D000535:Aluminum; D019204:GTP-Binding Proteins; D005459:Fluorides