[Effects of experimental Listeria monocytogenes infection on mice fed on lamprey or sardine oil prepared under high-temperature deodorization].
Fresh lamprey (F-La) or sardine (F-Sa) oil is known to contain a large amount of n-3 polyunsaturated fatty acids such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. When F-La or F-Sa was deodorized with steam at 280 degrees C under 1 mmHg for 1 h (H-La and H-Sa, respectively), the contents of EPA and DHA were reduced and unidentified peaks were newly detected by gas-liquid chromatography. To know the biological influences of these high-temperature deodorized oils, the sterilizing function of the macrophage against Listeria monocytogenes was investigated in male ddY mice fed H-La or H-Sa. One week feeding of H-La or H-Sa lowered the LD50 values of the bacteria injected intravenously. Numbers of the viable bacteria on the day 3 after intravenous injection were about 10 times higher in the liver and 5 times higher in the spleen of mice fed H-La or H-Sa as compared with those of the control group. These results suggest that the sterilizing function of fixed macrophages both in the liver and the spleen was suppressed in mice fed H-La or H-Sa.
Mineo
S
S
Department of Pharmacology, Kohno Clinical Medicine Research Institute, Tokyo, Japan.
Konishi
Y
Y
Satoh
K
K
Yanagisawa
A
A
Ishikawa
K
K
Kurata
M
M
D000818:Animals; D015169:Colony Count, Microbial; D005395:Fish Oils / Q000494:pharmacology*; D006358:Hot Temperature; D007928:Lethal Dose 50; D008087:Listeria / Q000472:pathogenicity; D008088:Listeriosis / Q000382:microbiology*; D008099:Liver / Q000382:microbiology; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D009812:Odorants; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects*; D013154:Spleen / Q000382:microbiology
D005395:Fish Oils