title: Detection of mycobacterial antigens in sputum by an enzyme immunoassay.
abstract: An enzyme immunoassay (EIA) for the detection of mycobacterial antigens in sputum was evaluated. The system utilises commercially available anti-BCG immunoglobulin. BCG protein standard was used as positive control. Thirty-nine patients with culture-proven pulmonary tuberculosis were tested. The EIA was positive in 24 of 29 patients with positive smears and cultures, giving a sensitivity of 86.2%. It was also positive in six of ten patients with smear-negative culture-positive disease, resulting in a sensitivity of 60% in this group. In another 176 patients with different nontuberculous pulmonary infections, only nine were positive by EIA, giving a specificity of 94.9%. The high sensitivity and specificity of the assay make it a useful tool for the early diagnosis of pulmonary tuberculosis.
authors.affiliation: Department of Medicine, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
authors.forename: I O
authors.initials: IO
authors.lastname: al-Orainey
authors.forename: M O
authors.initials: MO
authors.lastname: Gad el Rab
authors.forename: M S
authors.initials: MS
authors.lastname: al-Hajjaj
authors.forename: E S
authors.initials: ES
authors.lastname: Saeed
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D009163:Mycobacterium bovis / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
chemical_list: D000942:Antigens, Bacterial