Energy restriction and testosterone implants alter ornithine decarboxylase gene expression in kidney of female rats.
Effects of testosterone and energy restriction (30%) on enzyme activity and mRNA level of ornithine decarboxylase (ODC) were studied in 19-wk-old female Sprague-Dawley rats. Testosterone implantation for 1 wk elicited a sixfold increase in ODC activity and a fourfold increase in ODC mRNA transcripts in the kidney. Energy restriction also increased renal ODC activity. Further, the 56% increase in ODC enzyme activity observed in the testosterone-implanted, energy-restricted group was greater than the 24% increase in the placebo-implanted, energy-restricted group. No changes in renal ODC mRNA levels were observed in the energy-restricted groups. These observations suggest that translational or post-translational mechanism(s) are involved in the greater renal ODC activity in energy-restricted rats.
Baik
M
M
Department of Animal and Range Sciences, North Dakota State University, Fargo 58105.
Harrold
R L
RL
Choi
C B
CB
Slanger
W D
WD
Sung
C K
CK
Park
C S
CS
D000818:Animals; D004032:Diet; D004734:Energy Metabolism; D004958:Estradiol / Q000097:blood; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000097:blood / Q000494:pharmacology*
D012333:RNA, Messenger; D013739:Testosterone; D004958:Estradiol; D009955:Ornithine Decarboxylase