Application of the new zinc-62/copper-62 generator: an effective labeling method for 62Cu-PTSM.
A potential PET flow tracer, 62Cu-labeled pyruvaldehyde bis(N4-methylthiosemicarbazone) (62Cu-PTSM), was prepared using a new 62Zn/62Cu generator. With this 62Cu-labeling method based on a ligand exchange reaction, 62Cu-PTSM was quantitatively obtained by simple mixing of the generator eluate, 62Cu-glycine and PTSM solution for a few seconds. The glycine contained in the 62Cu-PTSM injectate had no significant effect in mouse biodistribution studies. The PET studies of 62Cu-PTSM showed brain images that were not visualized by 62Cu-labeled albumin (a plasma pool tracer), in the dog cranium.
Matsumoto
K
K
Department of Radiopharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Fujibayashi
Y
Y
Yonekura
Y
Y
Wada
K
K
Takemura
Y
Y
Konishi
J
J
Yokoyama
A
A
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006321:Heart / Q000000981:diagnostic imaging; D007202:Indicators and Reagents; D007553:Isotope Labeling; D008024:Ligands; D008297:Male; D051379:Mice; D009942:Organometallic Compounds / Q000493:pharmacokinetics; D013882:Thiosemicarbazones / Q000493:pharmacokinetics; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed; D015037:Zinc Radioisotopes
D007202:Indicators and Reagents; D008024:Ligands; D009942:Organometallic Compounds; D013882:Thiosemicarbazones; D015037:Zinc Radioisotopes; C052629:copper pyruvaldehyde bis(N(4)-methylthiosemicarbazone) complex