Carbonylmetalloimmunoassay (CMIA) a new type of non-radioisotopic immunoassay. Principles and application to phenobarbital assay.
A new non-radioisotopic immunoassay procedure, which we have termed carbonylmetalloimmunoassay (CMIA), is described. The tracers used in this approach are organometallic carbonyl complexes that can be detected at femtomole levels (300-700 fmol) by Fourier transform infrared (FT-IR) spectroscopy. The validity of the technique has been tested in a phenobarbital assay using as the marker a cyclopentadienylmanganese (I) tricarbonyl (cymantrene) moiety, ethyl acetate extraction to separate the free and bound organometallic fractions, and FT-IR spectroscopy to detect the CO stretching modes of the organometallic label. Typical dilution and standard curves obtained with this CMIA procedure are presented. The method was of comparable sensitivity to a [14C] radioimmunoassay (RIA) for the detection of phenobarbital. A comparison of the results for phenobarbital assays by both CMIA and RIA showed that higher titres were obtained using the CMIA method. The standard curves suggest that CMIA is a reliable and reproducible immunoassay procedure for phenobarbital.
Salmain
M
M
Ecole Nationale Supérieure de Chimie de Paris, U.A. C.N.R.S. 403, Paris, France.
Vessières
A
A
Brossier
P
P
Butler
I S
IS
Jaouen
G
G
D003429:Cross Reactions; D005583:Fourier Analysis; D009942:Organometallic Compounds; D010634:Phenobarbital / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods*; D012016:Reference Values; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared
D009942:Organometallic Compounds; D010634:Phenobarbital; C044679:manganese cyclopentadienyl tricarbonyl