Calorimetric study of interactions of toxin from Radianthus macrodactylus with erythrocyte membrane.
Differential scanning microcalorimetry, intrinsic protein fluorescence and SDS-electrophoresis have been applied for the study of the hemolytic toxin from sea anemone, Radianthus macrodactylus, when it is incorporated into pure sphingomyelin/water systems and upon its effect on the human and dog erythrocyte membranes. The results obtained by using these techniques showed that one molecule of toxin withdraws six sphingomyelin molecules from the cooperative transition. Effect of hemolytic toxin on human erythrocyte ghosts causes an appearance of a new heat sorption peak in their differential scanning calorimetric curve with a maximum of 36 degrees C and, moreover, it leads to a loss of one of cytoskeleton proteins (actin). These effects are essentially weaker in the case of dog erythrocyte ghosts. This suggests differences in the structural organization of human and dog erythrocyte membranes.
Shnyrov
V L
VL
Institute of Theoretical and Experimental Biophysics, USSR Academy of Sciences, Pushchino Moscow Region.
Monastyrnaya
M M
MM
Zhadan
G G
GG
Kuznetsova
S M
SM
Kozlovskaya
E P
EP
D000818:Animals; D002152:Calorimetry, Differential Scanning; D004285:Dogs; D004586:Electrophoresis; D004910:Erythrocyte Membrane / Q000187:drug effects*; D005453:Fluorescence; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D008387:Marine Toxins / Q000494:pharmacology*; D012615:Sea Anemones; D013109:Sphingomyelins / Q000378:metabolism
D008387:Marine Toxins; D013109:Sphingomyelins