Assembly and analysis of cosmid contigs in the CEA-gene family region of human chromosome 19.
The carcinoembryonic antigen (CEA)-like genes are members of a large gene family which is part of the immunoglobulin superfamily. The CEA family is divided into two major subgroups, the CEA-subgroup and the pregnancy-specific glycoprotein (PSG)-subgroup. In the course of an effort to develop a set of overlapping cosmids spanning human chromosome 19, we identified 245 cosmids in a human chromosome 19 cosmid library (6-7X redundant) by hybridization with an IgC-like domain fragment of the CEA gene. A fluorescence-based restriction enzyme digest fingerprinting strategy was used to assemble 212 probe-positive cosmids, along with 115 additional cosmids from a collection of approximately 8,000 randomly selected cosmids, into five contigs. Two of the contigs contain CEA-subgroup genes while the remaining three contigs contain PSG-subgroup genes. These five contigs range in size from 100 kb to over 300 kb and span an estimated 1 Mb. The CEA-like gene family was determined by fluorescence in situ hybridization to map in the q13.1-q13.2 region of human chromosome 19. Analysis of the two CEA-subgroup contigs provided verification of the contig assembly strategy and insight into the organization of 9 CEA-subgroup genes.
Tynan
K
K
Human Genome Center, Lawrence Livermore National Laboratory, Livermore, CA 94550.
Olsen
A
A
Trask
B
B
de Jong
P
P
Thompson
J
J
Zimmermann
W
W
Carrano
A
A
Mohrenweiser
H
H
D002272:Carcinoembryonic Antigen / Q000235:genetics*; D002888:Chromosomes, Human, Pair 19; D003360:Cosmids / Q000235:genetics*; D016172:DNA Fingerprinting; D015342:DNA Probes / Q000235:genetics; D005453:Fluorescence; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D005810:Multigene Family / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011257:Pregnancy Proteins / Q000235:genetics
D002272:Carcinoembryonic Antigen; D015342:DNA Probes; D006023:Glycoproteins; D007127:Immunoglobulin Constant Regions; D011257:Pregnancy Proteins