DNA damage in human white blood cells after inhalative exposure to methylenediphenyl diisocyanate (MDI)--case report.
A workplace-related inhalative exposure test in a challenge chamber was performed on an industrial worker in a methylenediphenyl diisocyanate (MDI) atmosphere from 5 to 20 ppb and the isolated white blood cell DNA was analysed by electrophoresis, anion-exchange chromatography and melting behaviour. The results of electrophoresis indicate that inhaled MDI induces double-strand breaks of DNA. Some of the DNA fragments were estimated to be in the region of 100-500 bp. Anion-exchange chromatography confirmed this finding. Following denaturation and rapid renaturation the results demonstrated that some DNA fragments are cross-linked by MDI. Comparing the melting curves before and after inhalative exposure in the challenge test chamber, genomic DNA revealed differences in the shape of the melting curve (hyperchromic effect). The results suggest that occupational MDI exposure could be associated with white blood cell DNA damage.
Marczynski
B
B
Research Institute of Occupational Medicine, Ruhr-University of Bochum, F.R.G.
Czuppon
A B
AB
Hoffarth
H P
HP
Marek
W
W
Baur
X
X
D000280:Administration, Inhalation; D001249:Asthma / Q000139:chemically induced; D001273:Atmosphere Exposure Chambers; D002852:Chromatography, Ion Exchange; D003485:Cyanates / Q000506:poisoning*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004591:Electrophoresis, Polyacrylamide Gel; D004781:Environmental Exposure; D006801:Humans; D017953:Isocyanates; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D001559:Benzhydryl Compounds
D003485:Cyanates; D004247:DNA; D017953:Isocyanates; C005969:4,4'-diphenylmethane diisocyanate; D001559:Benzhydryl Compounds