title: Extended secondary structure as a basis of increased RNA stability in a thermophilic alga Cyanidium caldarium.
abstract: To identify important structural features in the intergenic sequences of ribosomal DNAs, the nucleotide sequence of the 18-25S rRNA intergenic region was determined in a thermophilic alga, Cyanidium caldarium. Although the mature 5.8S RNA is more stable to thermal denaturation, sequence comparisons reveal a longer molecule with a surprisingly low G/C nucleotide composition. Estimates of the structure further indicate that, unlike other thermophilic examples, thermostability in this organism results, at least in part, from an extended secondary structure.
authors.affiliation: Department of Molecular Biology and Genetics, University of Guelph, Canada.
authors.forename: S A
authors.initials: SA
authors.lastname: Elela
authors.forename: R N
authors.initials: RN
authors.lastname: Nazar
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000737:chemistry*; D012337:RNA, Ribosomal, 18S / Q000737:chemistry*; D012340:RNA, Ribosomal, 5.8S / Q000737:chemistry*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D000461:Rhodophyta / Q000235:genetics*; D013696:Temperature
chemical_list: D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S; C065039:RNA, ribosomal, 25S