title: Dog gastric lipase: stimulation of its secretion in vivo and cytolocalization in mucous pit cells.
abstract: Dog gastric lipase (DGL) secretion is stimulated in vivo by urecholine, pentagastrin, histamine, 16,16-dimethyl prostaglandin E2, and secretin. Under fasting conditions, DGL is irreversibly inactivated by gastric acid below pH 1.5; consequently, DGL output can be underestimated. This problem has been resolved by buffering the acid or by using an antisecretory drug such as omeprazole during stimulation. There is a clear parallelism between the secretion of DGL and of gastric mucus. This observation led to the present investigation of the cellular localization of DGL using immunofluorescence techniques. Results showed that DGL is cytolocalized in mucous pit cells of gastric glands. Pepsinogen is found in chief cells. To the authors' knowledge, this is the first description of an enzyme (gastric lipase) secreted by mucous-type gastric cells. In contrast to other species, gastric lipase of the dog is located in cardiac, fundic, and antral mucosae.
authors.affiliation: Centre de Biochimie et de Biologie Moléculaire, Centre National de la Recherche Scientifique, Marseille, France.
authors.forename: F
authors.initials: F
authors.lastname: Carrière
authors.forename: V
authors.initials: V
authors.lastname: Raphel
authors.forename: H
authors.initials: H
authors.lastname: Moreau
authors.forename: A
authors.initials: A
authors.lastname: Bernadac
authors.forename: M A
authors.initials: MA
authors.lastname: Devaux
authors.forename: R
authors.initials: R
authors.lastname: Grimaud
authors.forename: J A
authors.initials: JA
authors.lastname: Barrowman
authors.forename: C
authors.initials: C
authors.lastname: Bénicourt
authors.forename: J L
authors.initials: JL
authors.lastname: Junien
authors.forename: R
authors.initials: R
authors.lastname: Laugier
mesh_terms: D000818:Animals; D001626:Bethanechol Compounds / Q000494:pharmacology; D004285:Dogs; D004795:Enzyme Stability; D005744:Gastric Acid / Q000378:metabolism; D005745:Gastric Acidity Determination; D005753:Gastric Mucosa / Q000166:cytology / Q000201:enzymology*; D006632:Histamine / Q000494:pharmacology; D007150:Immunohistochemistry; D008049:Lipase / Q000032:analysis / Q000378:metabolism*; D008297:Male; D010418:Pentagastrin / Q000494:pharmacology; D010435:Pepsinogens / Q000032:analysis; D012633:Secretin / Q000494:pharmacology
chemical_list: D001626:Bethanechol Compounds; D010435:Pepsinogens; D012633:Secretin; D006632:Histamine; D008049:Lipase; D010418:Pentagastrin