Confirmation of the human cathepsin B gene (CTSB) assignment to chromosome 8.
Human cathepsin B gene (CTSB) has been mapped to two locations: 8p22 and 13q14. Here we confirm the chromosome 8 assignment by three independent methods: (1) analysis of human-hamster somatic cell hybrid DNA by polymerase chain reaction; (2) comparison of hybridization signals to cathepsin B in interphase nuclei of normal fibroblasts and fibroblasts with a chromosome 8 deletion; and (3) fluorescence in situ hybridization to metaphase spreads using cathepsin B cosmid clones. Our results indicate that human CTSB is located at 8p22-p23.1.
Fong
D
D
Department of Biological Sciences, State University of New Jersey, Piscataway 08855-1059.
Chan
M M
MM
Hsieh
W T
WT
Menninger
J C
JC
Ward
D C
DC
D001483:Base Sequence; D002401:Cathepsin B / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D005453:Fluorescence; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D002401:Cathepsin B