title: On-line liquid chromatography/thermospray mass spectrometry in the analysis of oligosaccharides.
abstract: The analysis of intact neutral oligosaccharides by on-line liquid chromatography/thermospray mass spectrometry is described. Molecular-weight information on oligomers up to a degree of polymerization of 10 is obtained using an aqueous mobile phase containing 10(-4) mol/L sodium acetate, which was found to be compatible with thermospray interfacing and ionization. Ions due to sodiated and disodiated oligosaccharides are observed under these conditions without fragmentation. The aqueous 10(-4) mol/L sodium acetate mobile phase is demonstrated to be applicable in the separation of mixtures of oligosaccharides on a reversed-phase octadecyl-modified silica column.
authors.affiliation: Division of Analytical Chemistry, Center for Bio-Pharmaceutical Sciences, Leiden, The Netherlands.
authors.forename: W M
authors.initials: WM
authors.lastname: Niessen
authors.forename: R A
authors.initials: RA
authors.lastname: van der Hoeven
authors.forename: J
authors.initials: J
authors.lastname: van der Greef
authors.forename: H A
authors.initials: HA
authors.lastname: Schols
authors.forename: A G
authors.initials: AG
authors.lastname: Voragen
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D009844:Oligosaccharides / Q000032:analysis*
chemical_list: D009844:Oligosaccharides