[Contribution of bacterial detachment by enzymatic or physical methods to the diagnosis of foreign material infections and chronic osteomyelitis].
Bacterial adhesion is a crucial factor in the pathogenicity of numerous bacterial species. This property plays such a great role in infections of foreign material that removal of the material is the only effective therapy. The electron microscopy studies performed by Christensen have demonstrated that infected foreign material harbors bacteria which are often coated with slime and therefore escape detection by conventional microbiological methods. in view of these properties, enzymatic or physical detachment of adherent bacteria was attempted. 231 specimens from a variety of materials (catheters, heart valves, drains, bone biopsies) which yielded negative results after 15 days culture in Schaedler medium were either exposed to trypsin or sonicated. Among catheter specimens, 44.7% yielded positive bacteriologic results; pathogens recovered included 8.3% micrococci, 5.1% nongroupable streptococci, 1.7% group D streptococci, 2.9% Enterobacteria, and 23.3% coagulase-negative staphylococci. Among the bone specimens, the improvement in yield was 64.7%; except in one case, the pathogens recovered were consistent with previously initiated treatments. This bacterial detachment method should be studied in a larger number of specimens to determine its diagnostic value as compared with conventional techniques.
Etesse-Carsenti
H
H
Service des Maladies Infectieuses et Tropicales, Hôpital de l'Archet, Nice, France.
Masseyeff
M F
MF
Entenza
J
J
Monnot
F
F
Giaume
F
F
Barbarin
A
A
Rosset
M J
MJ
Argenson
C
C
Dellamonica
P
P
D001422:Bacterial Adhesion / Q000187:drug effects*; D001705:Bioprosthesis; D002408:Catheters, Indwelling / Q000009:adverse effects; D002908:Chronic Disease; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D006350:Heart Valve Prosthesis / Q000009:adverse effects; D006801:Humans; D066298:In Vitro Techniques; D010019:Osteomyelitis / Q000175:diagnosis* / Q000209:etiology / Q000382:microbiology; D012189:Retrospective Studies; D013010:Sonication; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000209:etiology / Q000382:microbiology; D013212:Staphylococcus epidermidis / Q000302:isolation & purification; D014357:Trypsin / Q000494:pharmacology*
D014357:Trypsin
Apport des méthodes de décrochage enzymatique ou physique des bactéries au diagnostic des infections sur matériel étranger et des ostéomyélites chroniques.