Human spermatozoa produce C16-platelet-activating factor.
Recent data indicate that human spermatozoa produce platelet-activating factor as determined by the rabbit platelet [3H]serotonin release bioassay. In this report, we examined by fast atom bombardment/mass spectrometry the molecular species of platelet-activating factor generated by these germ cells. Extracted spermatozoal samples that contained platelet-activating factor bioactivity underwent straight-phase high-performance liquid chromatography, and fractions which coeluted with authentic C16- and C18-platelet-activating factor standards were subjected to fast atom bombardment/mass spectrometry. Our mass spectral data indicate that human spermatozoa synthesize C16-platelet-activating factor but not C18-platelet-activating factor.
Sanwick
J M
JM
Department of Medicine, University of Washington, Seattle 98195.
Talaat
R E
RE
Kuzan
F B
FB
Geissler
F T
FT
Chi
E Y
EY
Henderson
W R
WR
D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D010972:Platelet Activating Factor / Q000737:chemistry*; D013094:Spermatozoa / Q000737:chemistry* / Q000648:ultrastructure
D005227:Fatty Acids; D010972:Platelet Activating Factor