title: Studies of an improved rhesus hematopoietic progenitor cell assay.
abstract: We have improved Rhesus monkey marrow cell growth in semisolid media by means of substituting supplemented calf serum for fetal bovine serum. The cloning efficiency of light-density marrow cells separated on 60% Percoll was 126 (+/- 54)/10(5) (n = 12, +/- SD), and for light-density peripheral blood cells 60 (+/- 46)/10(6) (n = 11). Thirty-five percent of the colonies were multilineage, whereas the remainder were unilineage colonies composed of erythrocytes, megakaryocytes, and neutrophilic or monocytic granulocytes. Unilineage megakaryocyte colonies comprised 12% of the total marrow progenitor cells. The [3H]TdR suicide index of marrow progenitor cells was 47% +/- 9% (n = 12). This progenitor cell assay should prove useful in preclinical studies of the effect of recombinant hematopoietic growth factors on the number and cycling status of Rhesus hematopoietic progenitor cells.
authors.affiliation: Emory University, Department of Medicine, Atlanta, Georgia.
authors.forename: E F
authors.initials: EF
authors.lastname: Winton
authors.forename: P C
authors.initials: PC
authors.lastname: Jacobs
authors.forename: S K
authors.initials: SK
authors.lastname: Rozmiarek
authors.forename: C P
authors.initials: CP
authors.lastname: Stahl
authors.forename: L A
authors.initials: LA
authors.lastname: Myers
authors.forename: E
authors.initials: E
authors.lastname: Liehl
authors.forename: R E
authors.initials: RE
authors.lastname: Stoll
authors.forename: D C
authors.initials: DC
authors.lastname: Anderson
authors.forename: H M
authors.initials: HM
authors.lastname: McClure
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002999:Clone Cells; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D066298:In Vitro Techniques; D008253:Macaca mulatta / Q000097:blood*; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism
chemical_list: D010980:Platelet Membrane Glycoproteins