Fibroblastoid cells derived from rat bone marrow and lung differ in their pattern of production of hemopoietic stimulators and inhibitors.
Blood cells develop in the bone marrow, controlled by a complex network of regulatory factors, some of which originate in the non-hemopoietic stroma. Previously we found that the predominant fibroblastoid (FB) cell growing in primary cultures of rat marrow bears surface antigens different from similar cells derived from certain other tissues. As determined by two monoclonal antibodies, the 'marrow type' is ST3+/ST4-, and the 'peripheral type' is ST3-/ST4+. Here we describe some of the functional differences between these two cell types. After primary cultures were depleted of hemopoietic elements and 'other' type FB, colony-stimulating activity was detected in the conditioned media (CM) of cells derived from marrow (ST3+), but not from lung (ST4+). Conversely, lung FB produced a CM that inhibited the clonogenic growth of normal marrow precursors. This substance (or substances) was produced by lung cells in the absence of exogenous stimulation, but by marrow cells only after they had been cultured in the presence of macrophage-derived products. The inhibitory activity did not have any measurable effect on murine blood cell precursors. We conclude that these isolated antibody-defined fibroblast subpopulations from different organs differ in their capacity to stimulate or inhibit normal hemopoietic precursors.
Wang
H
H
Department of Medicine, Royal Victoria Hospital, West Montreal, Canada.
Sullivan
A K
AK
D000818:Animals; D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D015938:Complement Membrane Attack Complex; D003165:Complement System Proteins; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism; D016298:Hematopoietic Cell Growth Factors / Q000096:biosynthesis*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D016130:Immunophenotyping; D008168:Lung / Q000166:cytology*; D008222:Lymphokines / Q000096:biosynthesis*; D051381:Rats; D011914:Rats, Inbred BN
D000911:Antibodies, Monoclonal; D003115:Colony-Stimulating Factors; D015938:Complement Membrane Attack Complex; D016298:Hematopoietic Cell Growth Factors; D008222:Lymphokines; C047610:SC5b-9 protein complex; C022173:stem cell inhibitory factor; D003165:Complement System Proteins