Involvement of microbodies in penicillin biosynthesis.
Penicillium chrysogenum strains were constructed which express a mutant acyltransferase lacking the putative targeting signal for microbody proteins. The mutated enzyme was located in vacuoles and in neighbouring cytoplasm. Although acyltransferase was expressed in vivo and was active in vitro, the mutants did not produce penicillin. The results demonstrate the involvement of microbodies in penicillin production.
Müller
W H
WH
Institute of Biomembranes, State University of Utrecht, Netherlands.
Bovenberg
R A
RA
Groothuis
M H
MH
Kattevilder
F
F
Smaal
E B
EB
Van der Voort
L H
LH
Verkleij
A J
AJ
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D005800:Genes, Fungal; D008830:Microbodies / Q000201:enzymology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000096:biosynthesis*; D010408:Penicillium chrysogenum / Q000201:enzymology* / Q000235:genetics; D010957:Plasmids; D015183:Restriction Mapping; D014169:Transformation, Bacterial
D046915:Penicillin-Binding Proteins; D010406:Penicillins; D000217:Acyltransferases; C018756:acyl-CoA-6-aminopenicillanic acid acyltransferase