title: cDNA cloning and sequencing of tobacco chloroplast ribosomal protein L12.
abstract: Tobacco chloroplast ribosomal protein L12 was isolated as a ssDNA-cellulose-binding protein from a chloroplast soluble protein fraction. Based on the N-terminal amino acid sequence of chloroplast L12, a cDNA clone was isolated and characterized. The precursor protein deduced from the DNA sequence consists of a transient peptide of 53 amino acid residues and a mature L12 protein of 133 amino acid residues. The chloroplast L12 protein was synthesized with a reticulocyte lysate and subjected to nucleic acid-binding assays. L12 synthesized in vitro does not bind to ssDNA, dsDNA nor ribonucleotide homopolymers, but it binds to cellulose matrix.
authors.affiliation: Center for Gene Research, Nagoya University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Itadani
authors.forename: M
authors.initials: M
authors.lastname: Sugita
authors.forename: M
authors.initials: M
authors.lastname: Sugiura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000737:chemistry*; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D010947:Plants, Toxic; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D014026:Nicotiana / Q000737:chemistry / Q000235:genetics*
chemical_list: D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12