448-detector optical recording system: development and application to Aplysia gill-withdrawal reflex.
The spatial resolution of a fast, multichannel, optical recording apparatus has been improved in an attempt to increase the completeness of optical recordings of neuron activity responsible for the Aplysia gill-withdrawal reflex. We developed a new optical apparatus, increasing the number of pixels to 448. Action potential activity from 168 to 192 neurons could be detected. Hence, the number of optically detected neurons increased about 2.5 times in comparison with a previously used 100- or 124-detector optical apparatus. Because optically detected action potential signals were shown to be due to the cell body of a neuron, it was possible to know its position as well as its approximate size. The distribution of the detected active neurons and those neurons that showed an apparent change in spike frequency to the siphon stimulation were determined.
Nakashima
M
M
Department of Biotechnology, Mitsubishi Electric Corporation, Hyogo, Japan.
Yamada
S
S
Shiono
S
S
Maeda
M
M
Satoh
F
F
D000200:Action Potentials; D000818:Animals; D001048:Aplysia; D005069:Evaluation Studies as Topic; D008839:Microelectrodes; D008856:Microscopy, Fluorescence / Q000379:methods / Q000592:standards*; D012018:Reflex; D012815:Signal Processing, Computer-Assisted