title: Suppression of proteoglycan synthesis in chondrocyte cultures derived from canine intervertebral disc.
abstract: Chondrocytes were dissociated enzymatically from canine nucleus pulposus. After attaining monolayer growth, the cells were subpassaged and incubated with sodium salicylate, indomethacin, or sodium meclofenamate (10(-2) M to 3 x 10(-6) M). When incubated with nonsteroidal anti-inflammatory drug concentrations mimicking therapeutic plasma levels, proteoglycan synthesis was significantly suppressed. After 24 hours of incubation, there was a 15% decrease in radioactive sulfate (35SO4) incorporation for salicylate (10(-3) M), a 29% decrease for indomethacin (3 x 10(-6) M), and a 75% decrease for sodium meclofenamate (10(-5) M). This inhibition was both dose- and time-dependent. Despite the effect of these nonsteroidal anti-inflammatory drugs on quantitative proteoglycan synthesis, there was no demonstrable effect of these drugs on the size of proteoglycan monomers or the degree to which proteoglycan aggregate formation occurred.
authors.affiliation: Department of Orthopaedics, Case Western Reserve University, Cleveland, Ohio.
authors.forename: J U
authors.initials: JU
authors.lastname: Yoo
authors.forename: R S
authors.initials: RS
authors.lastname: Papay
authors.forename: C J
authors.initials: CJ
authors.lastname: Malemud
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002356:Cartilage / Q000166:cytology* / Q000187:drug effects; D002478:Cells, Cultured; D004285:Dogs; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000166:cytology*; D011509:Proteoglycans / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D013462:Sulfur Radioisotopes
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D011509:Proteoglycans; D013462:Sulfur Radioisotopes