Establishment of two continuous T-cell strains from a single plaque of a patient with mycosis fungoides.
From a plaque biopsy of a patient with mycosis fungoides, two different continuous cell lines were established by including both IL-2 and IL-4 in the culture medium. Both continuous cell lines appeared with characteristic chromosome markers after approximately 40 cell population doublings. The initial karyotype recognized in T cells from the skin biopsy was 46,XY and the karyotypes of the continuous cell strains were 46,XY, -18, + i(18q) and another with multiple chromosome aberrations as described in Sezary T-cell leukemia. Phenotyping with monoclonal antibodies and T-cell receptor analysis indicates that the latter cell strain represents a minority of T-cells in the plaque. Due to its many chromosomal aberrations it probably represents the malignant cell, which may be a central cell in the immune stimulation taking place in the skin.
Kaltoft
K
K
Institute of Human Genetics, Aarhus University, Denmark.
Bisballe
S
S
Dyrberg
T
T
Boel
E
E
Rasmussen
P B
PB
Thestrup-Pedersen
K
K
D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division; D002869:Chromosome Aberrations; D003470:Culture Media; D006801:Humans; D007376:Interleukin-2; D015847:Interleukin-4; D007621:Karyotyping; D008297:Male; D009182:Mycosis Fungoides / Q000276:immunology / Q000473:pathology*; D011948:Receptors, Antigen, T-Cell; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D015847:Interleukin-4