Monoclonal antibodies to a specific 54-kilodalton antigen of Nocardia spp.
Two monoclonal antibodies (MAbs) of the immunoglobulin G2A isotype, reacting with a Nocardia-specific 54-kDa antigen, were generated. As determined by Western blot (immunoblot), both MAbs reacted only with the 54-kDa band. As determined by indirect immunofluorescence or enzyme immunoassay with whole microorganisms, the MAbs did not react with Nocardia cells. One of the MAbs showed weak cross-reactivity with mycobacterial antigens, while the other showed no cross-reactivity.
Boiron
P
P
Unité de Mycologie, Institut Pasteur, Paris, France.
Stynen
D
D
Belkacem
G
G
Goris
A
A
Provost
F
F
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000737:chemistry; D006801:Humans; D007159:Immunologic Tests / Q000592:standards / Q000706:statistics & numerical data; D008970:Molecular Weight; D009615:Nocardia / Q000276:immunology*; D009617:Nocardia Infections / Q000175:diagnosis; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial