Deletion of mouse t-complex distorter-1 produces an effect like that of the t-form of the distorter.
An allele of the mouse brachyury locus, T22H, had been shown previously to involve a deletion of several markers in the proximal part of chromosome 17, and almost certainly includes deletion of the t-complex distorter gene Tcd-1. The effects of T22H on transmission ratio distortion and male sterility caused by the t-complex were compared with those of a partial t-haplotype th51, which carries the t-form of the distorter Tcd-1t. In combination with the complete haplotype tw32, T22H caused severe impairment of male fertility, but males of genotype T22H/t6 or T22H/th51 were normally fertile. These results were very similar to those obtained when th51 was in combination with the same haplotypes. In effect on transmission ratio T22H was again similar to th51, in that it produced a marked increase in the transmission of the haplotype t6. To test whether the effects of T22H were due to deletion of elements other than Tcd-1, the effect of T22H on transmission of the partial haplotype th2 was compared with that of the deletion Thp. Again T22H markedly increased transmission of the t-haplotype and the effect was significantly greater than the small effect produced by Thp. It is concluded that deletion of the distorter Tcd-1 has an effect like that of the t-form of this distorter, Tcd-1t, and hence that Tcd-1t must be an amorph or hypomorph. It is speculated that other t-complex distorters, Tcd-2t and Tcd-3t, may also be amorphs or hypomorphs.(ABSTRACT TRUNCATED AT 250 WORDS)
Lyon
M F
MF
MRC Radiobiology Unit, Didcot, Oxon, UK.
D000818:Animals; D002872:Chromosome Deletion; D005260:Female; D005298:Fertility / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins / Q000235:genetics*; D013735:Testicular Hormones / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region
D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D013735:Testicular Hormones; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases