title: Zinc plays both structural and catalytic roles in metalloproteins.
abstract: Zinc is bound to the catalytic site of zinc-dependent hydrolases by three amino acid residues, commonly histidines and glutamic acid, and to the structural site of gene transcription regulators by cysteine and histidine. Site-directed mutagenesis of even one catalytic-site ligand destroys enzyme activity without changing physical properties of the protein, including immunoreactivity. However, nutritional studies demonstrating loss of transcriptional activity upon zinc deprivation have not been reported.
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia 65211.
authors.forename: B L
authors.initials: BL
authors.lastname: O'Dell
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D008667:Metalloproteins / Q000737:chemistry*; D008968:Molecular Conformation; D015032:Zinc / Q000378:metabolism / Q000502:physiology*
chemical_list: D000596:Amino Acids; D008667:Metalloproteins; D015032:Zinc