Receptor-mediated formation of multilayer aggregates of primary cultured adult rat hepatocytes on lactose-substituted polystyrene.
We used a lactose-substituted polystyrene, poly-N-p-vinylbenzyl-D-lactonamide (PVLA), as a substratum for adult rat hepatocytes in primary culture. Spherical-shaped hepatocytes attached on PVLA substratum formed stable multilayer aggregates anchored on substratum through the stimulation of epidermal growth factor (EGF). The cells required calcium ion essentially to form the aggregates. The formation of multilayer aggregates was inhibited by colchicine, but not by cytochalasin B. The inhibition was also observed by added PVLA molecules in the culture medium and by treating surfaces of PVLA-coated dishes with allo A lectin. It was suggested that adult rat hepatocytes attached on PVLA substratum required the specific interaction between asialoglycoprotein receptors on the cell surface and PVLA substratum to form anchored multilayer aggregates.
Tobe
S
S
Kanagawa Academy of Science and Technology, Kawasaki-shi, Japan.
Takei
Y
Y
Kobayashi
K
K
Akaike
T
T
D000818:Animals; D037263:Asialoglycoprotein Receptor; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D001517:Coleoptera; D003571:Cytochalasin B / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D006027:Glycosides; D007785:Lactose / Q000031:analogs & derivatives*; D037102:Lectins; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008858:Microscopy, Phase-Contrast; D011137:Polystyrenes; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000187:drug effects / Q000502:physiology*
C045794:Allomyrina lectin; D037263:Asialoglycoprotein Receptor; D006027:Glycosides; D037102:Lectins; D011137:Polystyrenes; D011971:Receptors, Immunologic; D003571:Cytochalasin B; D004815:Epidermal Growth Factor; C074112:poly-N-4-vinylbenzyllactonamide; D007785:Lactose; D003078:Colchicine