Cloning of a DNA fragment involved in pigment production in Streptomyces avermitilis.
Streptomyces avermitilis has the ability to synthesize a diffusible, brown, melanin-like pigment, a common property among many Streptomyces species. A region of the S. avermitilis chromosome involved in the production of this pigment was cloned in Escherichia coli. Production of the brown pigment was attained in E. coli, and is optimal when medium is supplemented with copper ions, tyrosine and IPTG. The cloned S. avermitilis pigment-producing DNA fragment is under the control of the lac promoter carried in the E. coli vector. The gene involved in pigment production could be used as a tool to analyse gene expression in S. avermitilis, and as an alternative cloning marker in Streptomyces-Escherichia coli vectors.
Tibbetts
M W
MW
Department of Fermentation Process Research, Central Research Division, Pfizer, Groton, Connecticut 06340.
Hafner
E W
EW
Morgenstern
M R
MR
Skinner
D D
DD
Denoya
C D
CD
D003001:Cloning, Molecular; D003300:Copper / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D007544:Isopropyl Thiogalactoside / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics*; D014443:Tyrosine / Q000378:metabolism
D004269:DNA, Bacterial; D008543:Melanins; D007544:Isopropyl Thiogalactoside; D014443:Tyrosine; D003300:Copper