An enzymatic method for the estimation of L-leucine in rat blood.
A specific enzymatic method for the routine measurement of L-leucine in blood samples is presented. The method uses a commercial preparation of tRNAs and amino acetyl-tRNA synthetases for the specific loading of L-leucine into the tRNA(Leu) present, competing with carrier-free L-[U-14C]leucine. The radioimmunoassay-like plot of radioactivity found in the acid-insoluble (tRNA) fraction was used to determine the amount of unlabelled L-leucine of the samples when compared against a standard curve. The interference of L-isoleucine, L-valine and L-alanine was very low.
Latres
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E
Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Spain.
Fernández-López
J A
JA
Remesar
X
X
Alemany
M
M
D000409:Alanine / Q000097:blood; D000604:Amino Acyl-tRNA Synthetases; D000818:Animals; D007532:Isoleucine / Q000097:blood; D007930:Leucine / Q000097:blood*; D012356:RNA, Transfer, Leu; D051381:Rats; D011919:Rats, Inbred Strains; D012588:Scintillation Counting / Q000379:methods; D014633:Valine / Q000097:blood
D012356:RNA, Transfer, Leu; D007532:Isoleucine; D000604:Amino Acyl-tRNA Synthetases; D007930:Leucine; D014633:Valine; D000409:Alanine