title: Efficacy of tobramycin-soaked collagen shields vs tobramycin eyedrop loading dose for sustained treatment of experimental Pseudomonas aeruginosa-induced keratitis in rabbits.
abstract: We compared the efficacy of a fortified tobramycin-soaked collagen shield to the efficacy of a single loading dose (four 50-microliters drops) of fortified tobramycin eyedrops in the treatment of New Zealand White rabbits with Pseudomonas aeruginosa-induced keratitis. Eyedrop loading-dose efficacy was evaluated with and without lateral tarsorrhaphy. Six hours after a single treatment, significantly fewer Pseudomonas colonies were present in the corneas of all three drug-treated groups as compared to the number of colonies in the corneas of balanced salt solution-treated control rabbits (P less than .006). Although no significant difference was observed between any of the drug-treated groups, lateral tarsorrhaphy was associated with a greater than tenfold decrease in the number of colony-forming units (P = .073). We found no significant difference in efficacy between a collagen shield presoaked in tobramycin, and a single loading dose of tobramycin eyedrops, in the treatment of rabbits with P. aeruginosa-induced keratitis.
authors.affiliation: Bethesda Eye Institute, Department of Ophthalmology, St. Louis University, Missouri.
authors.forename: K K
authors.initials: KK
authors.lastname: Assil
authors.forename: S R
authors.initials: SR
authors.lastname: Zarnegar
authors.forename: B D
authors.initials: BD
authors.lastname: Fouraker
authors.forename: D J
authors.initials: DJ
authors.lastname: Schanzlin
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001684:Biological Dressings; D003094:Collagen; D015169:Colony Count, Microbial; D003315:Cornea / Q000378:metabolism / Q000382:microbiology; D004337:Drug Carriers; D015818:Eye Infections, Bacterial / Q000188:drug therapy*; D005143:Eyelids / Q000601:surgery; D007634:Keratitis / Q000188:drug therapy* / Q000382:microbiology; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D011817:Rabbits; D014031:Tobramycin / Q000008:administration & dosage* / Q000493:pharmacokinetics
chemical_list: D004337:Drug Carriers; D003094:Collagen; D014031:Tobramycin