A visual pigment from chicken that resembles rhodopsin: amino acid sequence, gene structure, and functional expression.
The amino acid sequence of a rhodopsin-like visual pigment from chickens has been determined by isolating and sequencing its gene. The predicted sequence is between 70% and 80% identical to bovine, human, and chicken rhodopsins and between 40% and 50% identical to human blue, green, and red cone pigments, the chicken red cone pigment, and cavefish long-wave cone pigments. The encoded pigment, produced by transfection of cDNA into cultured cells, absorbs maximally at 495 nm as determined from photobleaching difference spectra and reacts at 20 degrees C with 50 mM hydroxylamine with a half-time of 16 min. These properties, together with a high pI predicted from the amino acid sequence, suggest that this cloned gene encodes the chicken green pigment previously identified by biochemical and spectroscopic studies. This sequence defines a new branch of the visual pigment gene family.
Wang
S Z
SZ
Wilmer Ophthalmological Institute, Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Adler
R
R
Nathans
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002645:Chickens / Q000235:genetics*; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D019811:Hydroxylamine; D006898:Hydroxylamines; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D010777:Photochemistry; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012243:Rhodopsin / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013053:Spectrophotometry; D014162:Transfection
D006898:Hydroxylamines; D019811:Hydroxylamine; D004247:DNA; D012243:Rhodopsin