Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: II. The primary structures of the low molecular weight carbohydrate chains of the glycoproteins.
The primary structures of the O-glycosidically linked oligosaccharides isolated from glycoproteins GP I and GP II of Fusarium sp. M7-1 were established. The oligosaccharides released by alkaline borohydride treatment from the glycoproteins were purified by Bio-Gel P-4 and HPLC. This approach resulted in one monosaccharide and seven oligosaccharides. Their primary structures were resolved mainly by NMR spectrometry in combination with methylation mass spectrometry and fast atom bombardment mass spectrometry. The following structures have been determined. [formula: see text].
Jikibara
T
T
Department of Bioresource Science, Faculty of Agriculture, Kagawa University.
Tada
K
K
Takegawa
K
K
Iwahara
S
S
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005670:Fusarium / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D014574:Uronic Acids / Q000032:analysis*
D006023:Glycoproteins; D014574:Uronic Acids