Two forms of human milk bile-salt-stimulated lipase.
This study provides the first direct evidence for the existence of two active forms of the human milk enzyme bile-salt-stimulated lipase. Heparin-based affinity chromatography and size-exclusion chromatography were used to obtain two active forms of this enzyme with molecular masses of 97 and 120 kDa.
Swan
J S
JS
Department of Chemistry, Bucknell University, Lewisburg, PA 17837.
Hoffman
M M
MM
Lord
M K
MK
Poechmann
J L
JL
D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification*; D008049:Lipase / Q000302:isolation & purification*; D008895:Milk, Human / Q000201:enzymology*; D011480:Protease Inhibitors / Q000494:pharmacology; D002787:Sterol Esterase
D007527:Isoenzymes; D011480:Protease Inhibitors; C054784:bile salt-stimulated lipase; D002787:Sterol Esterase; D008049:Lipase