title: Rapid and sensitive determination of urinary 2,5-hexanedione by reversed-phase high-performance liquid chromatography.
abstract: A rapid and sensitive method for the determination of 2,5-hexanedione (HD) (the principal metabolite of n-hexane) in urine samples by reversed-phase high-performance liquid chromatography (HPLC) is described. The sample preparation procedure was based on solid-liquid extraction after acid hydrolysis; it was optimized to enable accurate HD determination in less than 30 min. Analysis of spiked real samples showed a recovery of more than 85% at the 0.1-ppm level, with a relative standard deviation of 5% and a detection limit as low as 0.01 ppm. Intra-assay and inter-assay coefficients of variation at the 0.5-ppm level were 4 and 5%, respectively. The chromatographic peak assigned to HD was identified by collecting the HPLC eluate at the retention time of HD and analysing it using Fourier transform infrared spectrometry coupled with high-resolution gas chromatography. Urine samples of unexposed and exposed subjects were analysed following the proposed analytical procedure. HPLC and high-resolution gas chromatographic analyses were also compared on these samples. A correlation factor of 0.992 was obtained, which showed a good agreement between the two sets of data.
authors.affiliation: Dipartimento di Chimica e Chimica Industriale, Pisa, Italy.
authors.forename: M P
authors.initials: MP
authors.lastname: Colombini
authors.forename: P
authors.initials: P
authors.lastname: Carrai
authors.forename: R
authors.initials: R
authors.lastname: Fuoco
authors.forename: C
authors.initials: C
authors.lastname: Abete
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006588:Hexanones / Q000652:urine*; D006801:Humans
chemical_list: D006588:Hexanones; C011269:2,5-hexanedione