title: Cloning and characterization of a human angiotensin II type 1 receptor.
abstract: A human liver cDNA library was screened using a rat type 1 angiotensin II receptor cDNA coding sequence as a probe. cDNA clones were isolated which encoded a protein of 359 amino acids that shared 94.4% and 95.3% identify to rat and bovine type 1 angiotensin II receptors, respectively. Ligand binding studies of the cloned receptor expressed in COS cells suggested that it is pharmacologically a type 1 angiotensin II receptor subtype. Electrophysiological studies of the receptor expressed in Xenopus laevis oocytes revealed that it could functionally couple to a second messenger system leading to the mobilization of intracellular stores of calcium. Southern and Northern blot analyses indicated that the cloned receptor is represented as a single copy in the human genome and is expressed in many tissues of different histogenic origin with the exception of brain, where mRNA transcripts were barely detectable.
authors.affiliation: Department of Molecular Genetics, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406.
authors.forename: D J
authors.initials: DJ
authors.lastname: Bergsma
authors.forename: C
authors.initials: C
authors.lastname: Ellis
authors.forename: C
authors.initials: C
authors.lastname: Kumar
authors.forename: P
authors.initials: P
authors.lastname: Nuthulaganti
authors.forename: H
authors.initials: H
authors.lastname: Kersten
authors.forename: N
authors.initials: N
authors.lastname: Elshourbagy
authors.forename: E
authors.initials: E
authors.lastname: Griffin
authors.forename: J M
authors.initials: JM
authors.lastname: Stadel
authors.forename: N
authors.initials: N
authors.lastname: Aiyar
mesh_terms: D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000235:genetics*; D001483:Base Sequence; D001667:Binding, Competitive; D015152:Blotting, Northern; D015139:Blotting, Southern; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006801:Humans; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D011945:Receptors, Angiotensin / Q000145:classification / Q000235:genetics*; D015398:Signal Transduction; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D002118:Calcium