Induction of Calbindin-D28K by 1,25-dihydroxyvitamin D3 in cultured chicken intestinal cells.
Intestinal cells from chicken embryos were grown in chemically defined, serum-free medium. The majority of cultured cells exhibits an epithelial-like morphology. As demonstrated by indirect immunofluorescence, the epithelial cells, and not the contaminating fibroblasts, express Calbindin-D28K only after 1,25-dihydroxyvitamin D3, the hormonally active form of vitamin D, is added to the culture medium. The highly sensitive reverse transcriptase-polymerase chain reaction shows that both Calbindin-D28K mRNA and the corresponding primary unprocessed transcripts (pre-mRNA) are dramatically increased in cultured intestinal cells treated with 1,25-dihydroxyvitamin D3, thus indicating that Calbindin-D28K is induced by the increased rate of transcription of the corresponding gene.
Ferrari
S
S
Istituto di Chimica Biologica, Università di Modena, Italy.
Molinari
S
S
Battini
R
R
Cossu
G
G
Lamon-Fava
S
S
D000818:Animals; D001483:Base Sequence; D064026:Calbindins; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D066298:In Vitro Techniques; D007422:Intestines / Q000196:embryology* / Q000502:physiology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis*
D064026:Calbindins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G; D002117:Calcitriol