Reduced ciliary neuronotrophic factor-like activity in nerves from diabetic or galactose-fed rats.
This study examined the levels of ciliary neuronotrophic factor (CNTF)-like activity in the sciatic nerve of rats with short-term streptozotocin-induced diabetes or dietary supplementation with 40% galactose. CNTF-like activity, found in nerve extracts by in vitro bioassay, was reduced to 20% of control values after 1 or 2 months of galactose feeding (both P less than 0.01) and to 70% of control values after two months of streptozotocin diabetes (P less than 0.01). These data demonstrate that short-term hyperglycemia or its consequences can reduce extractable levels of Schwann cell-derived neuronotrophic factor and raise the possibility that impaired Schwann cell production of CNTF may contribute to the development of experimental diabetic neuropathy.
Calcutt
N A
NA
Department of Anesthesiology, University of California San Diego, La Jolla 92093.
Muir
D
D
Powell
H C
HC
Mizisin
A P
AP
D000818:Animals; D020934:Ciliary Neurotrophic Factor; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005260:Female; D005690:Galactose / Q000008:administration & dosage; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000378:metabolism*
D020934:Ciliary Neurotrophic Factor; D009419:Nerve Tissue Proteins; D005690:Galactose