title: An enzyme-linked immunosorbent assay for the detection of specific IgG antibody to Mycoplasma gallisepticum in sera and tracheobronchial washes.
abstract: A sensitive indirect ELISA is reported for the detection and quantitation of specific IgG to Mycoplasma gallisepticum (MG) in sera and tracheobronchial washes (TBW) of MG-infected chickens. The sensitivity of the assay was ensured by the use of mouse monoclonal antibody to chicken IgG bound to a prospective anti-MG containing sample that was complexed with MG antigen immobilized on a solid phase. The level of specific IgG antibody in a test sample was detected by using peroxidase-conjugated goat anti-mouse IgG. Serum samples with various levels of anti-MG IgG activity were used to construct a standard curve response at a single working dilution by using Logit-Log curve fitting. The assay was simple, reliable, and specific and was used to monitor the appearance of specific anti-MG IgG in chicken sera and TBW at various intervals after the onset of mycoplasma-induced respiratory disease. The IgG response reached a plateau at 2 and 4 weeks postinfection in TBW and sera, respectively; then the response waned but still was detectable at a significant level for up to 25 weeks postinfection.
authors.affiliation: Department of Avian Medicine, University of Georgia, Athens 30602.
authors.forename: M G
authors.initials: MG
authors.lastname: Elfaki
authors.forename: G O
authors.initials: GO
authors.lastname: Ware
authors.forename: S H
authors.initials: SH
authors.lastname: Kleven
authors.forename: W L
authors.initials: WL
authors.lastname: Ragland
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood; D000918:Antibody Specificity; D001980:Bronchi / Q000276:immunology; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014132:Trachea / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G