Preparation of heparin-glyceryl controlled-pore glass affinity media for the separation of alpha- and beta-lipoproteins.
The preparation of a stable affinity medium with heparin as the affinity ligand has been investigated. Glyceryl controlled-pore glass (CPG) was activated with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate and coupled with heparin. This affinity medium was used to separate some simple proteins, trypsinogen and lysozyme, in a high-performance liquid chromatographic configuration. The heparin-glyceryl-CPG was also used to separate alpha- and beta-lipoproteins in human serum. The effectiveness of the separation is confirmed by radial immunodiffusion and the determination of the cholesterol content of each of the separated fractions.
Carpenter
A
A
Department of Chemistry, McGill University, Montreal, Quebec, Canada.
Purdy
W C
WC
D002846:Chromatography, Affinity; D005898:Glass; D005990:Glycerol / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D005779:Immunodiffusion; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D009113:Muramidase / Q000302:isolation & purification; D014362:Trypsinogen / Q000302:isolation & purification
D008074:Lipoproteins; D014362:Trypsinogen; D006493:Heparin; D009113:Muramidase; D005990:Glycerol