title: Expression of chitin synthase genes during yeast and hyphal growth phases of Candida albicans.
abstract: Chitin, the beta 1,4-linked polymer of N-acetylglucosamine, is a fibrous polysaccharide that in many yeasts helps to maintain the structure of the mother-bud junction and in filamentous fungi is often the major supporting component of the cell wall. We have previously described a Candida albicans chitin synthase, CHS1. The DNA and derived protein sequences of a second gene, CHS2, are presented and compared with previously published gene sequences. Northern blot analysis shows that strikingly different levels of synthase 1 and 2 expression occur during yeast and hyphal phases of Candida growth.
authors.affiliation: Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: J L
authors.initials: JL
authors.lastname: Chen-Wu
authors.forename: J
authors.initials: J
authors.lastname: Zwicker
authors.forename: A R
authors.initials: AR
authors.lastname: Bowen
authors.forename: P W
authors.initials: PW
authors.lastname: Robbins
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D002473:Cell Wall / Q000201:enzymology; D002686:Chitin / Q000096:biosynthesis; D002687:Chitin Synthase / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data
chemical_list: D004271:DNA, Fungal; D002686:Chitin; D002687:Chitin Synthase