title: Characterization of the low density lipoprotein receptor activity in buffalo rat liver (BRL-3A) cells.
abstract: 1. BRL-3A cells possess a specific LDL receptor with an apparent mol. wt of 160,000 that binds, with saturation, both human and rat 125I-LDL. 2. Like human fibroblasts, BRL-3A cells also bind, internalize and degrade 125I-hLDL but to a lesser extent. 3. BRL-3A cells also bind the monoclonal antibody against rat liver LDL receptor P1B3. Moreover the LDL receptor activity increases when cells are preincubated with medium containing 5% of LPDS. 4. As with human (h) fibroblasts, treatment of BRL-3A cells with 10(-7) M insulin enhances binding (30%), internalization (18%) and degradation (20%) of 125I-hLDL.
authors.affiliation: Istituto di Scienze Fisiologiche Umane, Università di Napoli, Italia.
authors.forename: M
authors.initials: M
authors.lastname: Santillo
authors.forename: P
authors.initials: P
authors.lastname: Mondola
authors.forename: F
authors.initials: F
authors.lastname: Santangelo
authors.forename: A
authors.initials: A
authors.lastname: Belfiore
authors.forename: I
authors.initials: I
authors.lastname: Tedesco
authors.forename: C
authors.initials: C
authors.lastname: Laezza
authors.forename: R
authors.initials: R
authors.lastname: Cerbone
authors.forename: A
authors.initials: A
authors.lastname: Romano
authors.forename: M
authors.initials: M
authors.lastname: Bifulco
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009115:Muridae; D011973:Receptors, LDL / Q000378:metabolism*
chemical_list: D011973:Receptors, LDL