title: Fresh blood units contain large potent platelets that improve hemostasis after open heart operations.
abstract: Twenty units of fresh whole blood were separated into fresh packed red blood cells (PC) and platelet-rich plasma (PRP) and were transfused to 40 patients immediately after coronary bypass grafting. Patients were preoperatively randomized to receive either PRP (group A, 20 patients) or PC (group B, 20 patients). Platelet number in the PRP group was greater, but not significantly greater, than in the PC group (7.5 +/- 3 versus 5.9 +/- 2.2 x 10(10); p = not significant). However, mean platelet volume in the PC group was significantly greater (8.75 +/- 1.1 versus 6 +/- 0.7 fL). Postoperatively, group A patients bled more than group B (566 +/- 164 versus 327 +/- 41 mL; p less than 0.01) and received more red blood cell units (2.7 +/- 1.2 versus 1.6 +/- 0.7 U; p less than 0.05) and a larger number of blood products (5.9 +/- 3.7 versus 2.6 +/- 1.2 U; p less than 0.05). Transfusion of PRP to group A increased platelet count from 128 +/- 20 to 148 +/- 110 x 10(9)/L; however, platelet functions did not improve. Administration of PC to group B increased platelet count from 139 +/- 22 to 156 +/- 23 x 10(9)/L, improved platelet aggregation (with collagen from 33% +/- 20% to 53% +/- 23%, with epinephrine from 36% +/- 24% to 51% +/- 20%; p less than 0.05), and corrected the prolonged bleeding time. The results suggest that the improved hemostasis observed after fresh whole blood administration is related to the large, potent platelets that remained in the PC and were not separated to the PRP during standard platelet concentrate preparation.
authors.affiliation: Department of Cardiac Surgery, Chaim Sheba Medical Center, Tel Hashomer, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Mohr
authors.forename: D A
authors.initials: DA
authors.lastname: Goor
authors.forename: A
authors.initials: A
authors.lastname: Yellin
authors.forename: Y
authors.initials: Y
authors.lastname: Moshkovitz
authors.forename: A
authors.initials: A
authors.lastname: Shinfeld
authors.forename: U
authors.initials: U
authors.lastname: Martinowitz
mesh_terms: D001769:Blood; D016063:Blood Loss, Surgical; D001792:Blood Platelets / Q000166:cytology; D001803:Blood Transfusion / Q000379:methods*; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass; D017707:Erythrocyte Transfusion; D005260:Female; D006400:Hematocrit; D006488:Hemostasis, Surgical / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000502:physiology; D010976:Platelet Count; D017713:Platelet Transfusion