title: Coulter counter identifies Cryptococcus neoformans as leukocytes. A case of pseudopleocytosis.
abstract: Cerebrospinal fluid from a 42-year-old man with acquired immune deficiency syndrome was processed in a Coulter S-plus counter and found to contain "4.8 x 10(9) leukocytes/L:56.1% granulocytes and 37.7% lymphocytes." Direct examination of the same specimen in a counting chamber revealed that the leukocytes were cells of Cryptococcus neoformans. Coulter analysis of cerebrospinal fluid may be inappropriate, particularly when yeast infection is a probable diagnosis.
authors.affiliation: Department of Microbiology, Tel Aviv Sourasky Medical Center, Israel.
authors.forename: S A
authors.initials: SA
authors.lastname: Berger
authors.forename: D A
authors.initials: DA
authors.lastname: Waitman
authors.forename: R
authors.initials: R
authors.lastname: Baruch
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000134:cerebrospinal fluid; D000328:Adult; D001772:Blood Cell Count / Q000295:instrumentation*; D002555:Cerebrospinal Fluid / Q000166:cytology / Q000382:microbiology; D003454:Cryptococcus / Q000302:isolation & purification*; D003951:Diagnostic Errors; D006801:Humans; D007962:Leukocytes / Q000473:pathology*; D008297:Male; D016919:Meningitis, Cryptococcal / Q000382:microbiology* / Q000473:pathology