Stimulation of osteoclastic bone resorption by hydrogen peroxide.
The molecular mechanisms underlying the pathophysiology of bone destruction still remain poorly understood. We have found that hydrogen peroxide (H2O2), a reactive oxygen species (ROS), is a potent stimulator of osteoclastic bone resorption and cell motility. A marked enhancement of bone resorption was noted when rat osteoclasts, cultured on devitalised bovine cortical bone, were exposed to 10 nM [H2O2]. Apart from exposing osteoclasts to a low extracellular pH, which is known to enhance osteoclastic bone resorption, we provide first evidence for a molecule that stimulates osteoclastic bone resorption in osteoclast cultures that do not respond to parathyroid hormone and 1, 25 dihydroxyvitamin D3. We envisage that both basic biological and practical clinical implications may eventually follow from these studies.
Bax
B E
BE
Bone and Mineral Metabolism Unit, St. George's Hospital Medical School, London, U.K.
Alam
A S
AS
Banerji
B
B
Bax
C M
CM
Bevis
P J
PJ
Stevens
C R
CR
Moonga
B S
BS
Blake
D R
DR
Zaidi
M
M
D000818:Animals; D000831:Animals, Newborn; D001862:Bone Resorption; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D002117:Calcitriol / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D002469:Cell Separation; D005269:Femur / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology; D051381:Rats; D013977:Tibia / Q000378:metabolism
D010281:Parathyroid Hormone; D006861:Hydrogen Peroxide; D002117:Calcitriol