title: Ribonucleoprotein components of root meristematic cell nuclei of the tomato characterized by application of mild loosening and immunocytochemistry.
abstract: Immunocytochemistry and hypotonic-formaldehyde fixation have been used to study the extranucleolar ribonucleoprotein (RNP) constituents of the nucleus of tomato root meristematic cells. The study of the distribution of small nuclear uridine-rich RNPs (snRNP) by means of a monoclonal anti-Sm antibody recognizing a 29-kDa protein in plants, after standard fixation, shows a preferential labeling of the perichromatin region and a lower labeling of the interchromatin space. These results suggest that in the tomato there is a perichromatin region similar to that of animal cells, in which much of the nonnucleolar transcription and splicing takes place. In hypotonic-formaldehyde-detergent-fixed nuclei, fibrogranular polyparticles have been visualized reacting with anti-snRNP antibody. These structures are frequently associated with filaments of extended chromatin characterized by their reaction with an anti-DNA monoclonal antibody.
authors.affiliation: Laboratory of Electron Microscopy, Faculty of Sciences, National Autonomous University of México, D.F.
authors.forename: G H
authors.initials: GH
authors.lastname: Vázquez-Nin
authors.forename: O M
authors.initials: OM
authors.lastname: Echeverría
authors.forename: A
authors.initials: A
authors.lastname: Mínguez
authors.forename: S
authors.initials: S
authors.lastname: Moreno Díaz de la Espina
authors.forename: S
authors.initials: S
authors.lastname: Fakan
authors.forename: T E
authors.initials: TE
authors.lastname: Martin
mesh_terms: D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000737:chemistry*; D005404:Fixatives; D007150:Immunohistochemistry; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000737:chemistry; D009994:Osmolar Concentration; D010940:Plant Proteins / Q000737:chemistry; D010944:Plants / Q000737:chemistry / Q000648:ultrastructure*; D012261:Ribonucleoproteins / Q000737:chemistry*
chemical_list: D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D005404:Fixatives; D009687:Nuclear Proteins; D010940:Plant Proteins; D012261:Ribonucleoproteins