Acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides, has phospholipase, lysophospholipase, diacylglycerollipase, and acyltransferase activities in vitro.
Human acyloxyacyl hydrolase (AOAH) is a leukocyte enzyme that hydrolyzes acyloxyacyl bonds in the lipid A region of bacterial lipopolysaccharide (LPS), thereby detoxifying the LPS. We report here that the enzyme also acts in vitro on glycerophospholipids, lysophospholipids, and diacylglycerol. While AOAH preferentially removes palmitate or stearate from the sn-1 position of phospholipid and diacylglycerol substrates that have unsaturated acyl chains in the sn-2 position, it is able to cleave both palmitates from sn-1,2-dipalmitoylphosphatidylcholine and sn-1,2-dipalmitoylglycerol. This apparent preference for removing saturated (or shorter) acyl chains from glycerolipids is consistent with its ability to cleave laurate more rapidly than palmitoleate from lipopolysaccharide (Erwin, A. L., and Munford, R. S. (1990) J. Biol. Chem. 265, 16444-16449). AOAH also catalyzes acyl transfer from LPS and phosphatidylethanolamine to acceptor lipids; approximately equal amounts of laurate and myristate are transferred from LPS to monooleoylglyceryl ether, forming acyloleoylglyceryl ether. The demonstration that AOAH has phospholipase, lysophospholipase, diacylglycerol lipase, and acyltransferase activities in vitro suggests that the enzyme may have roles in addition to LPS deacylation (detoxification) in phagocytic cells.
Munford
R S
RS
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
Hunter
J P
JP
D000217:Acyltransferases / Q000378:metabolism*; D002122:Calcium Chloride / Q000494:pharmacology; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute; D007962:Leukocytes / Q000201:enzymology*; D008070:Lipopolysaccharides / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008245:Lysophospholipase / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D013379:Substrate Specificity
D008070:Lipopolysaccharides; D000217:Acyltransferases; D010740:Phospholipases; D002265:Carboxylic Ester Hydrolases; C048964:acyloxyacyl hydrolase; D008071:Lipoprotein Lipase; D008245:Lysophospholipase; D002122:Calcium Chloride