Development and characterization of a whole-cell radioligand binding assay for [125I]gp120 of HIV-1.
The binding of HIV-1 envelope glycoprotein, gp120, to the CD4 receptor is an important step in productive infection. The development of agents which interrupt this binding phenomenon should be of therapeutic interest. The present study characterizes a whole cell gp120/CD4 radioligand binding assay (radioligand binding assay) modified for use in a high volume screening format. Modifications include the use of human CD4 receptor stably expressed in a Chinese hamster ovary cell line and the gentle fixation (paraformaldehyde) of the CD4 receptor just prior to assay. Binding of [125I]gp120 to fixed CD4 was of high affinity (KD = 6 nM), saturable, reversible, and specific. The kinetics of binding were identical to those of viable (non-fixed) CD4 receptor. [125I]gp120 binding was inhibited by unlabeled recombinant gp120, soluble CD4, and the anti-CD4 monoclonals OKT4A and LEU3A. A number of compounds reported to inhibit gp120 binding and/or gp120 induced syncytium formation were also active in this assay. This modified radioligand binding assay was developed to initiate a rational and extensive screening program to assist in the identification of potential chemotherapeutic agents based on their ability to inhibit gp120 binding to host cells.
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NovaScreen, Division of Nova Pharmaceutical Corp., Baltimore, Maryland 21224.
Balasubramanian
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Charlton
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Francis
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Doyle
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Sweetnam
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D000818:Animals; D001667:Binding, Competitive; D015704:CD4 Antigens / Q000378:metabolism; D016466:CHO Cells / Q000378:metabolism; D006224:Cricetinae; D005069:Evaluation Studies as Topic; D015699:HIV Envelope Protein gp120 / Q000032:analysis* / Q000378:metabolism; D015497:HIV-1; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D011869:Radioligand Assay / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism
D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D007457:Iodine Radioisotopes; D011994:Recombinant Proteins