Flow cytometric analysis of DNA content and RAS P21 oncoprotein expression in ovarian neoplasms.
Flow cytometric analysis of DNA content and ras p21 expression were studied in paraffin-embedded normal ovary (NO, n = 10), serous cystadenoma (SA, n = 11), serous tumors of low malignant potential (LMP, n = 13), and papillary serous cystadenocarcinoma (SCa, n = 7). Tissue for DNA analysis was processed via a modified Hedley technique; a separate aliquot of the same sample was stained with a monoclonal antibody directed to oncoprotein ras p21 (clone Y13259). NO (10/10) and SA (11/11) demonstrated diploid DNA stemlines; 4/12 LMP and 5/7 SCa were aneuploid. S-phase fraction varied with significant differences (p less than 0.001) for SA (mean = 4.4%), LMP (mean = 9.0%), and SCa (mean = 12.7%). When all cases were evaluated, p21 expression was relatively lower in NO and SA (mean = 4.2%) in contrast to LMP and SCa (mean = 13.0% and 8.1%). Diploid cases were examined separately, and lesions with high p21 expression were associated with a diagnosis of LMP/SCa (p less than 0.001), whereas diploid tissues with low p21 expression were associated with a nonmalignant diagnosis (NO/SA). This study suggests that aneuploidy or diploidy with high p21 expression (greater than 10%) may be associated with LMP or frankly malignant ovarian tumors.
Kotylo
P K
PK
Department of Pathology, Indiana University School of Medicine, Indianapolis 46202-5200.
Michael
H
H
Fineberg
N
N
Sutton
G
G
Roth
L M
LM
D000782:Aneuploidy; D003536:Cystadenocarcinoma / Q000737:chemistry / Q000235:genetics; D003537:Cystadenoma / Q000737:chemistry / Q000235:genetics; D004247:DNA / Q000032:analysis*; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015689:Oncogene Protein p21(ras) / Q000032:analysis*; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000235:genetics; D011003:Ploidies
D004247:DNA; D015689:Oncogene Protein p21(ras)