Oligonucleotide fingerprinting to individualize ungulates.
The optimal combination of restriction enzyme and oligonucleotide probe has been determined for the individualization of hoofed animal species (cattle, pig, goat, sheep, horse and camel). Four different restriction endonucleases were used as well as five synthetic oligonucleotide probes hybridizing to different simple tandem repeats for fingerprint analyses in unrelated cattle (Swiss and German Simmental of unknown relationship and three families): 4 x 10(15) cows and oxes would reveal different banding patterns after HinfI digestion using the probe (CAC)5/(GTG)5. The other species were investigated using HinfI (and HaeIII) and five different oligonucleotide probes specific for simple tandem repeats. Using (CAC)5/(GTG)5 the discrimination potential in sheep was about one order of magnitude lower than in cattle while in goats 6 x 10(10) specimen are easily differentiated with (CA)8/(GT)8. From an evolutionary standpoint it may be of interest that also in all other ungulate species tested, (CAC)5/(GTG)5 and (CA)8/(GT)8 exhibited the highest potential for individualization. Advantages of oligonucleotide fingerprinting are discussed.
Schwaiger
F W
FW
Max-Planck-Institut für Psychiatrie, Martinsried, Germany.
Gomolka
M
M
Geldermann
H
H
Zischler
H
H
Buitkamp
J
J
Epplen
J T
JT
Ammer
H
H
D000818:Animals; D001188:Artiodactyla / Q000235:genetics*; D016172:DNA Fingerprinting; D016678:Genome; D009710:Nucleotide Mapping; D015345:Oligonucleotide Probes; D010334:Paternity
D015345:Oligonucleotide Probes