title: Assignment of the human TYRP (brown) locus to chromosome region 9p23 by nonradioactive in situ hybridization.
abstract: The TYRP (brown) locus determines pigmentation and coat color in the mouse. The human homolog of the TYRP locus has been recently identified and shown to encode a 75-kDa transmembrane melanosomal glycoprotein called gp75. The gp75 glycoprotein is homologous to tyrosinase, an enzyme involved in the synthesis of melanin, forming a family of tyrosinase-related proteins. A genomic clone of human gp75 was used to map the human TYRP locus to chromosome 9, region 9p23, by nonradioactive fluorescent in situ hybridization. Specificity of hybridization was tested with a genomic fragment of human tyrosinase that mapped to a distinct site on 11q21. The 9p region has been reported to be nonrandomly altered in human melanoma, suggesting a role for the region near the TYRP locus in melanocyte transformation.
authors.affiliation: Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
authors.forename: V V
authors.initials: VV
authors.lastname: Murty
authors.forename: B
authors.initials: B
authors.lastname: Bouchard
authors.forename: S
authors.initials: S
authors.lastname: Mathew
authors.forename: S
authors.initials: S
authors.lastname: Vijayasaradhi
authors.forename: A N
authors.initials: AN
authors.lastname: Houghton
mesh_terms: D002899:Chromosomes, Human, Pair 9; D005453:Fluorescence; D006801:Humans; D008545:Melanoma / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics*; D014442:Monophenol Monooxygenase / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010858:Pigmentation / Q000235:genetics*; D012680:Sensitivity and Specificity; D012689:Sequence Homology, Nucleic Acid
chemical_list: D008562:Membrane Glycoproteins; D014442:Monophenol Monooxygenase