Inhibition of fibroblast growth by polyanions; effects of dextran sulfate and lignin derivatives.
When macrophages prepared from rat bone marrow were cultured for more than one week, contaminating fibroblasts grew and formed large colonies which were observable with the naked eye after Giemsa staining. The addition of dextran sulfate or water-soluble lignin derivatives (acetyl or sulfonyl) to the culture medium almost completely inhibited the growth of fibroblasts at 10 micrograms/ml. These polyanions also inhibited the growth of chick embryo fibroblasts in a dose-dependent manner: at 5 micrograms/ml the inhibitory effect was 20-30% of the control and at 100 micrograms/ml it was 50-70%. In addition, these polyanions inhibited the mitosis of hepatocytes, although their inhibitory effects on the growth of hepatocytes were smaller than those observed on the growth of fibroblasts. On the other hand, these polyanions did not affect the growth of human urinary bladder carcinoma cells (HUB-4 and HUB-15). In conclusion, dextran sulfate and lignin derivatives may be useful in eliminating fibroblasts from certain cells.
Sorimachi
K
K
Department of Microbiology, Dokkyo University School of Medicine, Tochigi, Japan.
Watanabe
K
K
Yamazaki
S
S
Yasumura
Y
Y
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D002642:Chick Embryo; D016264:Dextran Sulfate / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D008031:Lignin / Q000494:pharmacology* / Q000031:analogs & derivatives; D008264:Macrophages / Q000187:drug effects; D008297:Male; D008938:Mitosis / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured / Q000187:drug effects
D006131:Growth Inhibitors; C001545:lignosulfuric acid; D008031:Lignin; D016264:Dextran Sulfate