cDNA cloning and chromosome assignment of the gene for human brain 14-3-3 protein eta chain.
We present the nucleotide sequence of a cDNA clone of mRNA encoding human 14-3-3 protein, a protein kinase-dependent activator of tyrosine and tryptophan hydroxylases and an endogenous inhibitor of protein kinase C. The 1,730-nucleotide sequence of the cloned cDNA contains 191 bp of a 5'-noncoding region, the complete 738 bp of coding region, and 801 bp of a 3'-noncoding region containing three canonical polyadenylation signals. The 14-3-3 protein eta chain cDNA encoded a polypeptide of 246 amino acids with a predicted molecular weight 28,196. The predicted amino acid sequence of human 14-3-3 protein eta was highly homologous to that of previously reported bovine and rat 14-3-3 proteins with only two amino acid differences. The sequence carries structural features as putative regions responsible for activation of tyrosine and tryptophan hydroxylases and for inhibition of Ca2+/phospholipid-dependent protein kinase C. Northern blot analysis demonstrated widespread expression of the 14-3-3 protein eta chain in cultured cell lines derived from various human tumors. These findings suggest the conservative functions of the 14-3-3 protein among species. Spot blot hybridization analysis with flow-sorted chromosomes showed that the human 14-3-3 protein eta chain gene is assigned to chromosome 22.
Ichimura-Ohshima
Y
Y
Research Laboratory for Molecular Genetics, Niigata University, Japan.
Morii
K
K
Ichimura
T
T
Araki
K
K
Takahashi
Y
Y
Isobe
T
T
Minoshima
S
S
Fukuyama
R
R
Shimizu
N
N
Kuwano
R
R
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000201:enzymology*; D002460:Cell Line; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D005260:Female; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D010920:Placenta / Q000502:physiology; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D015342:DNA Probes; D046911:Macromolecular Substances; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D004247:DNA; D014446:Tyrosine 3-Monooxygenase; D010751:Phosphopyruvate Hydratase