Identification of a novel secreted glycoprotein of the yeast Saccharomyces cerevisiae stimulated by heat shock.
A new secreted yeast glycoprotein with an Mr of about 400 kDa (gp400) has been found. The glycoprotein is an O-mannosylated oligomer, whose synthesis and export into culture medium are stimulated by heat shock. Intracellular transport of gp400 is carried out by membrane vesicles distinct from the known constitutive secretory vesicles. Immunological analysis revealed gp400 only in Saccharomyces species.
Lupashin
V V
VV
Institute of Biochemistry and Physiology of Microorganisms, U.S.S.R.
Kononova
S V
SV
Ratner YeN
Tsiomenko
A B
AB
Kulaev
I S
IS
D000596:Amino Acids / Q000032:analysis; D003594:Cytoplasmic Granules / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006031:Glycosylation; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006358:Hot Temperature; D008854:Microscopy, Electron; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure
D000596:Amino Acids; D005656:Fungal Proteins; D006023:Glycoproteins; D006360:Heat-Shock Proteins