Inhibitory effect of conjugated dienoic derivatives of linoleic acid and beta-carotene on the in vitro growth of human cancer cells.
The effects of physiologic concentrations of conjugated linoleic acid (CLA) and beta-carotene were assessed on human (M21-HPB, malignant melanoma; HT-29, colorectal; MCF-7, breast) cancer cells. The incubation of cancer cells with CLA showed significant reductions in proliferation (18-100%) compared to control cultures. M21-HPB and MCF-7 cell mortality was dose- and time-dependent. beta-Carotene was inhibitory to breast cells only. MCF-7 cells supplemented with CLA incorporated significantly less [3H]leucine (45%), [3H]uridine (63%) and [3H]thymidine (46%) than control cultures. M21-HPB and HT-29 cells supplemented with CLA incorporated less [3H]leucine (25-30%). These in vitro results suggest that CLA and beta-carotene may be cytotoxic to human cancer cells in vivo.
Shultz
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Department of Food Science and Human Nutrition, Washington State University, Pullman 99164-6376.
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Seaman
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Luedecke
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D001943:Breast Neoplasms; D002338:Carotenoids / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D003110:Colonic Neoplasms; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008041:Linoleic Acids / Q000494:pharmacology*; D008545:Melanoma; D009363:Neoplasm Proteins / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014407:Tumor Cells, Cultured; D014529:Uridine / Q000378:metabolism; D019207:beta Carotene
D004273:DNA, Neoplasm; D008041:Linoleic Acids; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D019207:beta Carotene; D014316:Tritium; D002338:Carotenoids; D007930:Leucine; D013936:Thymidine; D014529:Uridine