title: Calcitonin-induced changes in the organization of sulfatide-containing membranes.
abstract: The interactions of salmon calcitonin with glycosphingolipid sulfatide are studied by right angle light scattering from the lipid suspension, by the excimer to monomer ratio (E/M) of the fluorescence intensity of pyrene phosphatidylcholine and pyrene sulfatide and by the leakage of carboxyfluorescein. It was found that calcitonin strongly modified the structure of the sulfatide aggregate, as indicated by the light scattering determinations. At a lipid peptide ratio 100:1 (molar ratio) light scattering from the suspension was negligible, indicating the formation of peptide-sulfatide complexes with a structure different from that of the lipid aggregate. The interactions of calcitonin with sulfatide when the latter is a component of a bilayer were also evaluated. A specific calcitonin-membrane sulfatide interaction was demonstrated by determining the temperature-dependent E/M of pyrene phosphatidylcholine and pyrene sulfatide in dipalmitoyl phosphatidylcholine/sulfatide (80:20, molar ratio) liposomes. The E/M curves were modified by calcitonin only when the liposomes were labelled with fluorescent sulfatide which probes the sulfatide behavior in the membrane. Furthermore, the addition of calcitonin to the incubation medium of liposomes containing sulfatide promoted the release of vesicle entrapped carboxyfluorescein without disrupting the bilayer structure, the release being correlated with the amount of sulfatide in the bilayer and the calcitonin concentration in the medium.
authors.affiliation: Department of Medical Chemistry and Biochemistry, Faculty of Medicine, University of Milano, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Viani
authors.forename: G
authors.initials: G
authors.lastname: Cervato
authors.forename: P
authors.initials: P
authors.lastname: Gatti
authors.forename: B
authors.initials: B
authors.lastname: Cestaro
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism; D000818:Animals; D002116:Calcitonin / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002850:Chromatography, Gel; D005452:Fluoresceins; D006028:Glycosphingolipids / Q000378:metabolism; D008027:Light; D010713:Phosphatidylcholines / Q000378:metabolism; D012474:Salmon; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013433:Sulfoglycosphingolipids / Q000378:metabolism*
chemical_list: D005452:Fluoresceins; D006028:Glycosphingolipids; D010713:Phosphatidylcholines; D013433:Sulfoglycosphingolipids; D015060:1,2-Dipalmitoylphosphatidylcholine; C024098:6-carboxyfluorescein; D002116:Calcitonin