Some chemical properties of the HCl-methanol extract from the puparial cuticle of Drosophila melanogaster.
1. The HCl-methanol (HCl-MeOH) soluble fraction from the puparial cuticle of yellow, black and ebony of D. melanogaster was hydrolyzed in hydrochloric acid and examined for beta-alanine, ketocatechol, and acetic acid. 2. Between beta-alanine and ketocatechol and between beta-alanine and acetic acid, a quantitatively inverse relationship was found, respectively. The former relationship was further confirmed by the feeding experiment of beta-alanine to black. 3. Of total beta-alanine in the HCl-MeOH extract, the proportion of those having free amino group was 74.8 per cent. 4. All these results indicate that the HCl-MeOH soluble fraction of the puparial cuticle may be useful for investigating the cross-link structure of the cuticle.
Umebachi
Y
Y
Department of Biology, Faculty of Science, Kanazawa University, Japan.
Fukada
K
K
Nishio
Y
Y
Tagi
Y
Y
Aoki
M
M
Mizuno
C
C
Kizawa
K
K
Kurata
K
K
D000085:Acetates / Q000032:analysis; D000818:Animals; D002396:Catechols / Q000032:analysis; D002457:Cell Extracts; D004331:Drosophila melanogaster / Q000737:chemistry* / Q000166:cytology / Q000196:embryology; D006851:Hydrochloric Acid; D000432:Methanol; D011679:Pupa / Q000737:chemistry; D013057:Spectrum Analysis; D015091:beta-Alanine / Q000032:analysis
D000085:Acetates; D002396:Catechols; D002457:Cell Extracts; D015091:beta-Alanine; D006851:Hydrochloric Acid; D000432:Methanol