Cisplatin-induced imbalances in the pattern of chimeric marker gene expression in HeLa cells.
The effects of the antitumor drug cisplatin on gene expression have been measured during transient transfections in HeLa cells. The results indicate a surprising diversity of response. Expression from two promoters is strongly induced, both in non-replicating plasmids and cellular integrants, whereas expression from two other promoters is strongly inhibited. The results suggest that a drug-induced imbalance in gene expression may contribute to the antitumor properties of cisplatin.
Evans
G L
GL
Department of Chemistry and Biochemistry, University of California, Los Angeles 90024.
Gralla
J D
JD
D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D002945:Cisplatin / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D010957:Plasmids / Q000187:drug effects; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection
D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase; D002945:Cisplatin