Molecular cloning of dihydrolipoamide acetyltransferase of the rat pyruvate dehydrogenase complex: sequence comparison and evolutionary relationship to other dihydrolipoamide acyltransferases.
A complementary DNA (cDNA) clone of dihydrolipoamide acetyltransferase (E2) of the rat pyruvate dehydrogenase complex (PDC) was isolated from a lambda gt11 rat heart cDNA library. The amino acid sequence of a full mature protein of rat PDC-E2 was predicted by combination of the cDNA nucleotide sequence and the N-terminal amino acid sequence determined chemically. The amino acid sequence of rat PDC-E2 was well consistent with those of the E2 components of other alpha-ketoacid dehydrogenase complexes. These E2 components possess the sequence G-X-G-X-X-G, which is the consensus sequence for nucleotide binding sites of nucleotide binding proteins, in the E3 and/or E1 binding domains. The E2 components of the three alpha-ketoacid dehydrogenase complexes are suggested to be classified into three clusters separated during evolution.
Matuda
S
S
Department of Biology, Kanoya National Institute of Fitness and Sports, Japan.
Nakano
K
K
Ohta
S
S
Shimura
M
M
Yamanaka
T
T
Nakagawa
S
S
Titani
K
K
Miyata
T
T
D000123:Acetyltransferases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D015723:Gene Library; D008954:Models, Biological; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology*; D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping
D011768:Pyruvate Dehydrogenase Complex; D004247:DNA; D000123:Acetyltransferases; D051047:Dihydrolipoyllysine-Residue Acetyltransferase