Steroid 6 beta-hydroxylase and 6-desaturase reactions catalyzed by adrenocortical mitochondrial P-450.
The minor steroid hydroxylase activity of purified bovine adrenocortical mitochondrial P-450 is described. The results indicate that both P-450scc and P-450(11 beta) act on deoxycorticosterone and androstenedione to form 6 beta-hydroxydeoxycorticosterone and 6 beta-hydroxyandrostenedione (6 beta-hydroxylase), respectively. Both forms of P-450 also catalyze 6-desaturation of androstenedione to form 4,6-androstadiene-3,17-dione (6-desaturase).
Mochizuki
H
H
Department of Chemistry, Faculty of Science, Kanazawa University, Japan.
Suhara
K
K
Katagiri
M
M
D000302:Adrenal Cortex / Q000201:enzymology* / Q000648:ultrastructure; D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008928:Mitochondria / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000378:metabolism*
D000735:Androstenedione; D003900:Desoxycorticosterone; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D013250:Steroid Hydroxylases; C022657:steroid hormone 6-beta-hydroxylase; D013252:Steroid 11-beta-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme