Direct sequence analysis of the t(14;18) chromosomal translocation in Hodgkin's disease.
There have been conflicting reports about the occurrence of the t(14;18) chromosomal translocation in Hodgkin's disease. A polymerase chain reaction analysis of biopsy specimens from 21 patients with Hodgkin's disease (HD) has shown the presence of the t(14;18) translocation in four cases (20%). All four patients had nodular sclerosing HD. Direct sequencing of the amplified 14q+ junctions established that the BCL-2 (major breakpoint region) sequence was fused to an Ig joining region (JH) (J6 in all four cases). Different breakpoints were observed in each case but were similar in nature to the breakpoints described in follicular lymphoma. The exact nature and cell of origin in HD remains obscure, although the presence of the t(14;18) translocation may reflect either a B-cell origin in these cases or associated lymphoid hyperplasia.
Gupta
R K
RK
ICRF Department of Medical Oncology, St Bartholomew's Hospital, West Smithfield, London, UK.
Whelan
J S
JS
Lister
T A
TA
Young
B D
BD
Bodmer
J G
JG
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D001706:Biopsy; D002460:Cell Line; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006689:Hodgkin Disease / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D006801:Humans; D007133:Immunoglobulin Joining Region / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D014178:Translocation, Genetic
D004273:DNA, Neoplasm; D007133:Immunoglobulin Joining Region