title: Retinoic acid, hemin and hexamethylen bisacetamide interference with "in vitro" differentiation of chick embryo chondrocytes.
abstract: We have investigated the effect of all-trans Retinoic acid, and of substances (Hemine and Hexamethylene bisacetamide) which interfere with "in vitro" differentiation of mesenchyme derived cell lineages on the expression of specific markers of hyperthrophy in "in vitro" differentiating chick embryo chondrocytes. (Castagnola P., et al., 1986). Continuous treatment of chondrogenic cells in conditions allowing differentiation "in vitro" with Retinoic acid resulted in persistence of type I collagen synthesis and in lack of type X collagen and Ch 21 protein expression. Hemin treated cells secreted a reduced amount of type X collagen. HMBA treatment inhibited type X collagen expression and caused reduction of the ratio between type II collagen and Ch 21 synthesized. The data indicate an independent regulation of these markers during chondrocyte differentiation.
authors.affiliation: Istituto di Fisiologia Generale, Cattedra di Genetica, Genova, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Manduca
authors.forename: M L
authors.initials: ML
authors.lastname: Abelmoschi
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000818:Animals; D030161:Avian Proteins; D002352:Carrier Proteins / Q000032:analysis; D002356:Cartilage / Q000166:cytology / Q000196:embryology; D002454:Cell Differentiation / Q000187:drug effects*; D002642:Chick Embryo; D003094:Collagen / Q000032:analysis; D050556:Fatty Acid-Binding Proteins; D005786:Gene Expression Regulation / Q000187:drug effects; D006427:Hemin / Q000494:pharmacology*; D054834:Lipocalins; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D000081:Acetamides; D030161:Avian Proteins; D002352:Carrier Proteins; C066299:Ch21 protein, chicken; D050556:Fatty Acid-Binding Proteins; D054834:Lipocalins; D014212:Tretinoin; D006427:Hemin; D003094:Collagen; C014026:hexamethylene bisacetamide