Immunocharacterization of delta- and zeta-isoenzymes of protein kinase C in rat renal mesangial cells.
The isoforms of protein kinase C (PKC) present in rat mesangial cells were identified by immunoblot analysis with antibody raised against isotype-specific peptides. In addition to the previously observed alpha- and epsilon-subspecies, mesangial cells also express the delta- and zeta-isoenzymes of PKC. On exposure to phorbol 12,13-dibutyrate (PDB) a complete depletion of PKC-delta is observed within 8 h. Removal of PDB results in a recovery of PKC-delta. In contrast, PKC-zeta is unaffected by addition or removal of PDB.
Huwiler
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A
Research Department, Ciba-Geigy Ltd., Basel, Switzerland.
Fabbro
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D
Stabel
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S
Pfeilschifter
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D003429:Cross Reactions; D005920:Glomerular Mesangium / Q000201:enzymology*; D007527:Isoenzymes / Q000737:chemistry*; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011493:Protein Kinase C / Q000737:chemistry*; D011817:Rabbits; D051381:Rats
D007527:Isoenzymes; D010455:Peptides; D011493:Protein Kinase C