Isolation of a novel cDNA encoding a zinc-finger protein that binds to two sites within the c-myc promoter.
The ME1a1 and ME1a2 elements are cis-acting DNA sequences that exist at positions -46 and -85, respectively, within the P2 promoter of the c-myc gene. These elements are required for optimal transcription initiation from P2. The proteins that bind to these elements are identical or very similar. Here we have isolated a cDNA clone that encodes the carboxy-terminal 494 amino acids of the human ME1a1/ME1a2 factor. This factor is referred to as "ZF87" (zinc-finger protein, 87 kilodaltons). ZF87 specifically binds the ME1a1 element with higher affinity than the ME1a2 element. Western blot analysis indicates that the full-length protein has a relative molecular mass of approximately 87,000 daltons, and Northern blot analysis shows that it is encoded by a 5-kb transcript. ZF87 contains six zinc-finger domains, of the Cys2-His2 type, at the carboxy terminus of the protein. The protein also contains extended tracts of polyalanine.
Pyrc
J J
JJ
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Moberg
K H
KH
Hall
D J
DJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D015153:Blotting, Western; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D016259:Genes, myc; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D016335:Zinc Fingers / Q000235:genetics*
D004247:DNA