Renal hemodynamics and urinary concentrating capacity in protein deprivation: role of antidiuretic hormone.
The role of antidiuretic hormone (ADH) in the renal concentration defect and hemodynamic changes in protein malnutrition was evaluated in rats with diabetes insipidus (DI) after 2 weeks of low protein feeding. Free water reabsorptive capacity (TcH2O), glomerular filtration rate (GFR), and renal plasma flow (RPF) were measured in the protein deprived rats and in DI rats fed a normal protein diet. The effect of urea supplementation of the low protein diet on renal concentrating capacity was also evaluated. In addition, the renal hemodynamic response to acute administration of ADH was measured and correlated with changes in plasma renin concentration and renal renin content (RRC). Protein deprivation in DI rats resulted in reduced urine osmolality and urea excretion, differences which were reversed by urea supplementation. Protein deprivation did not affect free water reabsorptive capacity but did reduce GFR and RPF. Acute ADH administration significantly increased GFR and RPF in protein-deprived rats; these changes were associated with a reduction in RRC and release. These results suggest that dietary protein restriction does not directly affect the tubular capacity to generate and reabsorb free water. The hemodynamic changes seen in protein deprivation are not mediated by ADH and may be secondary to increased intrarenal angiotensin II.
Fernández-Repollet
E
E
Department of Pharmacology, University of Puerto Rico, San Juan.
Opava-Stitzer
S
S
Martínez-Maldonado
M
M
D000818:Animals; D003919:Diabetes Insipidus / Q000378:metabolism; D006439:Hemodynamics; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism; D007671:Kidney Concentrating Ability; D011488:Protein Deficiency / Q000503:physiopathology*; D051381:Rats; D011910:Rats, Brattleboro; D012083:Renin / Q000378:metabolism; D014508:Urea / Q000378:metabolism; D014667:Vasopressins / Q000494:pharmacology*
D014667:Vasopressins; D014508:Urea; D012083:Renin