Characterization of two galactosidases extracted from wheat germ with a hydroalcoholic solvent.
Alpha- and beta-D-galactosidases were characterized from a hydroalcoholic extract of wheat germ (Triticum vulgare). Kinetic constants (Vmax and KM) and the optimal pHs for the hydrolysis of p-nitrophenyl galactopyranosides by both enzymes were determined. These enzymes presented a high stability in hydroalcoholic medium and were inhibited by iodoacetamide and sodium p-hydroxy-mercuribenzoate.
Papet
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MP
Département de Biochimie des Glycoconjugués et des Lectines Endogènes, CNRS, Orléans, France.
Delay
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Monsigny
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Delmotte
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F
D000085:Acetates / Q000737:chemistry; D019342:Acetic Acid; D000431:Ethanol / Q000737:chemistry; D005696:Galactosidases / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014908:Triticum
D000085:Acetates; D000431:Ethanol; D005696:Galactosidases; D019342:Acetic Acid; C018824:ammonium acetate