Biosynthesis of androgens by the sesterterpene pathway via 23,24-dinor-4-cholen-3-one.
Rat testicular and adrenal gland microsomal preparations were incubated with 23,24-dinor-5-cholen-3 beta-ol (Guneribol) a proposed intermediate in the sesterterpene pathway for steroid biosynthesis. Steroids were isolated, purified by thin layer and high-performance liquid chromatography and crystallized to constant specific radioactivity. These preparations converted the substrate to 23,24-dinor-4-cholen-3-one. Radioactive 23,24-dinor-4-cholen-3-one was synthesized and incubated with further tissue preparations and shown to be converted to steroid hormones. These findings suggest that 23,24-dinor-4-cholen-3-one is an intermediate on the sesterterpene pathway for steroidogenesis.
Tait
A D
AD
Department of Paediatrics, University of Cambridge, Addenbrooke's Hospital, England.
D000311:Adrenal Glands / Q000378:metabolism*; D000728:Androgens / Q000096:biosynthesis*; D000735:Androstenedione / Q000096:biosynthesis / Q000302:isolation & purification; D000818:Animals; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D011283:Pregnenes / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis / Q000302:isolation & purification; D014316:Tritium
D000728:Androgens; D011283:Pregnenes; D014316:Tritium; C002241:20-methylpregn-5-en-3 beta-ol; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone