The yeast UME6 gene product is required for transcriptional repression mediated by the CAR1 URS1 repressor binding site.
URS1 is known to be a repressor binding site in Saccharomyces cerevisiae that negatively regulates expression of many genes including CAR1 (arginase), several required for sporulation, mating type switching, inositol metabolism, and oxidative carbon metabolism. In addition to the proteins previously shown to directly bind to the URS1 site, we show here that the UME6 gene product is required for URS1 to mediate repression of gene expression in the absence of inducer. We also show that mutations in the CAR80 (CARGRI) gene are allelic to those in UME6.
Park
H D
HD
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Luche
R M
RM
Cooper
T G
TG
D001119:Arginase / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013172:Spores, Fungal / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D001119:Arginase