Serologic diagnosis of Lyme disease using recombinant outer surface proteins A and B and flagellin.
Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.
Fikrig
E
E
Department of Internal Medicine, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06510.
Huguenel
E D
ED
Berland
R
R
Rahn
D W
DW
Hardin
J A
JA
Flavell
R A
RA
D000907:Antibodies, Bacterial / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005408:Flagellin / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008193:Lyme Disease / Q000175:diagnosis*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D005408:Flagellin