Detection of a novel carbon-phosphorus bond cleavage activity in cell-free extracts of an environmental Pseudomonas fluorescens isolate.
Cell-free extracts of Pseudomonas fluorescens strain 23F catalyzed the hydrolysis of phosphonoacetate to acetate and inorganic phosphate; the products were detected in almost equimolar quantities. The stable in vitro activity responsible was distinct from phosphonoacetaldehyde hydrolase and appears to represent a novel mode of carbon-phosphorus bond cleavage.
McMullan
G
G
School of Biology and Biochemistry, Queen's University, Belfast, Northern Ireland.
Quinn
J P
JP
D000085:Acetates / Q000378:metabolism; D002474:Cell-Free System; D006867:Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism; D010746:Phosphonoacetic Acid / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000254:growth & development / Q000378:metabolism*
D000085:Acetates; D010710:Phosphates; D006867:Hydrolases; C023920:phosphonoacetaldehyde hydrolase; D010746:Phosphonoacetic Acid