title: cDNA-derived amino-acid sequence of lentil seedlings' amine oxidase.
abstract: Lentil seedlings' amine oxidase (LSAO) cDNAs were identified in a cDNA lambda gt 10 library by plaques hybridization. The nucleotide sequence of a 2111 bp clone was determined. It contains part of a signal peptide, the complete sequence coding for the mature protein and the 3'-untranslated region of the mRNA. The deduced protein sequence shows that the mature protein is composed of 569 amino acids with a molecular mass of 67 kDa, also taking into account the glucidic component. The LSAO cDNA was identified by sequencing the N-terminal part of the protein and several tryptic peptides. The protein sequence shows a characteristic hexapeptide present in amine oxidases containing 6-hydroxydopa as the organic cofactor. Three conserved histidines might be the ligands of copper bound to the enzyme.
authors.affiliation: Dipartimenti di Medicina Sperimentale, Università di Roma Tor Vergata, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Rossi
authors.forename: R
authors.initials: R
authors.lastname: Petruzzelli
authors.forename: A F
authors.initials: AF
authors.lastname: Agrò
mesh_terms: D006631:Amine Oxidase (Copper-Containing); D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D007887:Fabaceae / Q000201:enzymology*; D008969:Molecular Sequence Data; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics*; D010946:Plants, Medicinal; D021382:Protein Sorting Signals / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D021382:Protein Sorting Signals; D012333:RNA, Messenger; D004247:DNA; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors