Barbiturates suppress glucose utilization by inhibition of hexokinase in neuroblastoma cells.
Exposure to the pentobarbital potently inhibited the 2-deoxy glucose uptake in cultured neuroblastoma cells. The inhibition was assumed to be due to saturation of the uptake in the early stage where the incorporation was linear in the nontreated cells. On the contrary, the incorporation of 3-O-methyl glucose, another glucose analog which is not phosphorylated by hexokinase, was not altered by the treatment with pentobarbital. These results suggest that the suppression of hexokinase is involved in the above-mentioned effect of pentobarbital.
Okazaki
H
H
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Tokuda
H
H
Ishii
K
K
Ishibashi
S
S
D019325:3-O-Methylglucose; D001463:Barbiturates / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D003847:Deoxyglucose / Q000378:metabolism*; D006593:Hexokinase / Q000037:antagonists & inhibitors*; D006801:Humans; D007700:Kinetics; D008757:Methylglucosides / Q000378:metabolism*; D009447:Neuroblastoma; D010424:Pentobarbital / Q000494:pharmacology*; D010852:Picrotoxin / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D019325:3-O-Methylglucose; D001463:Barbiturates; D003847:Deoxyglucose; D006593:Hexokinase; D008757:Methylglucosides; D010424:Pentobarbital; D010852:Picrotoxin; D005680:gamma-Aminobutyric Acid