Propagation of CAR bacillus in artificial media.
CAR bacillus propagated successfully in an artificial medium, and the number of CAR bacillus was about 30 times the original number after 8 days of cultivation. The medium consisted of Eagle's minimum essential medium supplemented with 10% fetal calf serum and 20% hamster tracheal organ culture soup. By intranasal inoculation to mice, two strains of the CAR bacillus passaged 5 and 6 times in this artificial medium produced the same lung lesions as natural CAR bacillus infection.
Shoji
Y
Y
Central Institute for Experimental Animals, Kanagawa, Japan.
Itoh
T
T
Kagiyama
N
N
D000818:Animals; D016863:Bacillaceae Infections / Q000382:microbiology; D001407:Bacillus / Q000254:growth & development* / Q000472:pathogenicity; D002923:Cilia; D003470:Culture Media; D051379:Mice; D008813:Mice, Inbred ICR; D012137:Respiratory System / Q000382:microbiology*; D012141:Respiratory Tract Infections / Q000382:microbiology
D003470:Culture Media