Neuromedin B is present in lung cancer cell lines.
Previously, high levels of gastrin-releasing peptide and its mRNA were detected in classic small cell lung cancer cell lines. Here the ability of lung cancer cell lines to synthesize neuromedin B (NMB), a structurally similar mammalian bombesin-like peptide, was investigated. By radioimmunoassay, NMB (0.1-0.7 pmol/mg of protein) was detected in 23 of 33 lung cancer cell lines. In contrast, gastrin-releasing peptide (0.1-12.9 pmol/mg of protein) was detected in 16 of 32 cell lines. Using gel filtration and high pressure liquid chromatography techniques, the main peak of immunoreactive NMB coeluted with synthetic NMB. By Northern analysis, a 0.8-kilobase mRNA species was present, using poly(A) mRNA derived from two of three lung cancer cell lines. Using a more sensitive S1 nuclease protection assay, NMB mRNA was present in most of the 15 lung cancer cell lines examined. These data suggest that NMB may be a regulatory peptide in lung cancer.
Giaccone
G
G
NCI-Navy Medical Oncology Branch, National Cancer Institute, Bethesda, Maryland.
Battey
J
J
Gazdar
A F
AF
Oie
H
H
Draoui
M
M
Moody
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TW
D015152:Blotting, Northern; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D015287:Neurokinin B / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D011863:Radioimmunoassay; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D015287:Neurokinin B; C038393:neuromedin B