Molecular cloning of curculin, a novel taste-modifying protein with a sweet taste.
cDNA clones for curculin, a novel taste-modifying protein, were isolated and sequenced. The encoded prepro-curculin was composed of 158 amino acid residues including a signal sequence of 22 residues and a carboxy-terminal extension peptide of 22 residues. Northern blot analysis showed that the mRNA for curculin was first detected in Curculigo latifolia fruits at 2 weeks after pollination and remained at a constant level for the following 4 weeks.
Abe
K
K
Department of Agricultural Chemistry, University of Tokyo, Japan.
Yamashita
H
H
Arai
S
S
Kurihara
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D010940:Plant Proteins; D012333:RNA, Messenger; C065630:curculin; D004247:DNA