A method to extract DNA for molecular studies from cells fixed in Carnoy.
We describe a simple method for isolation of high-molecular-weight DNA from cells fixed in Carnoy's solution for cytogenetic studies. DNA samples were extracted from NIH3T3 cells, and no apparent degradation was noticed. This method should be useful for molecular analysis of cells fixed for cytogenetic studies.
Barrios
L
L
Departament de Biologia Cellular i Fisiologia Universitat Autonoma de Barcelona, Spain.
Miró
R
R
Corominas
M
M
Pellicer
A
A
Egozcue
J
J
D016475:3T3 Cells; D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D002725:Chloroform; D004247:DNA / Q000302:isolation & purification*; D015245:Deoxyribonuclease BamHI; D004587:Electrophoresis, Agar Gel; D000431:Ethanol; D004996:Ethidium; D005404:Fixatives; D051379:Mice
D000085:Acetates; C033474:Carnoy's solution; D005404:Fixatives; D000431:Ethanol; D002725:Chloroform; D004247:DNA; D015245:Deoxyribonuclease BamHI; D004996:Ethidium; D019342:Acetic Acid