title: A rapid method for purification of herpesvirus DNA.
abstract: A rapid and reliable method for purification of herpesvirus DNA from cell cultures is described. The method is based on the isolation of virus particles and/or nucleocapsids by differential centrifugation and exploits the solubilizing and denaturing capabilities of cesium trifluoroacetate during isopycnic centrifugation, so that phenol/chloroform extractions can be omitted. The method was used for the purification of DNA from several members of the Alfaherpesvirinae subfamily.
authors.affiliation: State Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark.
authors.forename: L S
authors.initials: LS
authors.lastname: Christensen
authors.forename: P
authors.initials: P
authors.lastname: Normann
mesh_terms: D002478:Cells, Cultured; D004279:DNA, Viral / Q000302:isolation & purification*; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral