Ovarian cancer. Protocol for the preparation of banded metaphases from tumor tissue.
Protocols are presented for preparation of banded metaphases for cytogenetic analysis from short-term and long-term cultures of ovarian cancer. For short-term cultures (less than 24 hours of culture of freshly dissociated tumor material), prolonged exposure (16-18 hours) to mitotic inhibitors was required for metaphase isolation; shorter exposures to mitotic inhibitors (3-6 hours) were generally sufficient for ovarian cancer cells in culture for days to weeks (long-term cultures).
Chattopadhyay
S
S
Department of Pediatrics, Cornell University Medical College, New York, New York 10021.
Marinduque
B
B
Gilbert
F
F
D000970:Antineoplastic Agents / Q000494:pharmacology; D002871:Chromosome Banding; D005260:Female; D005404:Fixatives; D005821:Genetic Techniques; D006801:Humans; D008677:Metaphase / Q000187:drug effects*; D010051:Ovarian Neoplasms / Q000235:genetics*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D005404:Fixatives