Functional analysis of mononuclear cells infiltrating into tumors. VI. The effect of lymphocyte chemotactic factors on lymphocyte adhesion to endothelial cells.
We have analyzed mechanisms controlling infiltration of T lymphocytes into tumor tissues. A lymphocyte chemotactic factor-b (LMF-b) produced by tumor infiltrating CD4+ T lymphocytes was purified. LMB-b was specifically chemotactic for CD8+ T lymphocyte. Furthermore, LMF-b augmented lymphocyte adhesion to high endothelial venule (HEV) cells. The binding of CD8+ T cells to HEV cells was specifically augmented by LMF-b. The LMF-b primarily acted on T lymphocytes, whereas tumor necrosis factor as well as IFN-gamma acted on HEV cells or fibroblast cells. The binding of lymphocytes to fibroblast cell line was not augmented by LMF-b. The augmentation of lymphocyte adhesion to endothelial cells by LMF-b was mediated by the lymphocyte function associated antigen-1/intercellular adhesion molecule (LFA-1/ICAM) pathway, the CD2/LFA-3 pathway, and the very late antigen-4/culture supernatant-1 (VLA-4/CS-1) pathway.
Nomura
N
N
Department of Pathology, Sapporo Medical College, Japan.
Uno
E
E
Tamatani
T
T
Miyasaka
M
M
Suzuki
K
K
Suzuki
A
A
Kikuchi
K
K
Uede
T
T
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002630:Chemotactic Factors / Q000494:pharmacology* / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D007700:Kinetics; D016246:Lymphocytes, Tumor-Infiltrating / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology
D002630:Chemotactic Factors