Molecular characterization and cloning of sheep mitochondrial DNA.
Mitochondrial DNA from the liver of a single Rasa Aragonesa sheep has been isolated and characterized. The size of the genome, determined by restriction enzyme analysis, was found to be 16.58 kbp. The cleavage sites for the restriction endonucleases BamHI, HindIII, EcoRI, BglII, PvuII, BstEII and PstI were mapped, and the gene organization deduced through heterologous hybridization using different cloned fragments of the rat mitochondrial genome. Fragments representative of the entire sheep genome were cloned in plasmid vectors pGEM3Z and pUN121.
Villalta
M
M
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
Fernández-Silva
P
P
Beltrán
B
B
Enguita
L
L
López-Pérez
M J
MJ
Montoya
J
J
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015342:DNA Probes; D004272:DNA, Mitochondrial / Q000235:genetics*; D008099:Liver; D010802:Phylogeny; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000235:genetics*
D015342:DNA Probes; D004272:DNA, Mitochondrial