title: The structural basis of the poor fibrin specificity of urokinase(I)--knowledge-based prediction of kringle structures of urokinase and its related proteins.
abstract: The Kringle-1 structure of plasminogen (PGK-1), the Kringle-2 structure of tissue plasminogen activator (PAK-2) and the Kringle structure of prourokinase (UKK) has been modeled on the basis of the three-dimensional structure of Kringle-1 of prothrombin (PTK-1) at 2.8 A resolution. The predicted three-dimensional structure of these Kringles shows that the binding site of PGK-1 is characterized by an apparent dipolar site, the polar parts of which are separated by a hydrophobic region. PAK-2 possesses the anionic center but has not a cationic binding center which might be provided by a guanidinium group from Arg-69 located adjacent to the Arg-71 position. UKK possesses neither the anionic binding center nor the cationic center which are probably the main reason for the poor fibrin specificity of urokinase.
authors.affiliation: Department of Biochemistry, Nanjing University, PRC.
authors.forename: J N
authors.initials: JN
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Lu
authors.forename: X
authors.initials: X
authors.lastname: Gao
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: D X
authors.initials: DX
authors.lastname: Zhu
authors.forename: L H
authors.initials: LH
authors.lastname: Lai
authors.forename: X J
authors.initials: XJ
authors.lastname: Xu
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Tang
mesh_terms: D000595:Amino Acid Sequence; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010959:Tissue Plasminogen Activator / Q000235:genetics; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*
chemical_list: D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator