Fluid pinocytosis and esterase-oxidase in chronic myeloid leukemic granulocytes are differentially stimulated by chemotactic peptide.
Binding of a chemotactic peptide, n-formyl-methionyl-leucyl-phenylalanine (FMLP) to polymorphonuclear leukocytes (PMNL) leads to a series of biochemical and functional events. We have studied stimulation of fluid phase pinocytosis (FP), esterase and oxidase by FMLP in CML PMNL, by flow cytometry. Stimulation of FP in CML PMNL was lower than that in normal PMNL but, stimulation of esterase and oxidase was comparable to that in normal PMNL. Thus, early response to FMLP which is dependent on the integrity of actin network seems to be defective in CML PMNL.
Naik
N R
NR
Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
Advani
S H
SH
Bhisey
A N
AN
D002634:Chemotaxis, Leukocyte; D004950:Esterases / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood* / Q000201:enzymology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000201:enzymology / Q000502:physiology*; D010088:Oxidoreductases / Q000378:metabolism*; D010873:Pinocytosis
D009240:N-Formylmethionine Leucyl-Phenylalanine; D010088:Oxidoreductases; D004950:Esterases