Isolation of monochromosomal hybrids following fusion of human diploid fibroblast-derived microcells with mouse A9 cells.
Rodent x human hybrids containing single human chromosomes are valuable tools in the study of human genetics. Using a retrovirus carrying the neomycin gene, we generated a neomycin-resistant (neor) pool of human diploid fibroblasts. Following fusion of microcells prepared from this pool to mouse A9 cells, we obtained four neor hybrids containing a single human chromosome 2, 3, 5, or 15.
Ning
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Roy M. and Phyllis Gough Huffington Center on Aging, Baylor College of Medicine, Houston, TX 77030.
Lovell
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Taylor
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Pereira-Smith
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D000818:Animals; D002459:Cell Fusion / Q000235:genetics*; D002460:Cell Line; D002877:Chromosomes, Human; D002884:Chromosomes, Human, Pair 15; D002889:Chromosomes, Human, Pair 2; D002893:Chromosomes, Human, Pair 3; D002895:Chromosomes, Human, Pair 5; D004351:Drug Resistance / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D009355:Neomycin / Q000494:pharmacology
D009355:Neomycin