A solvent-free coating-procedure for the improved preparation of cryostat sections in light microscope histochemistry.
A new technique is presented for the external stabilization of cryostat sections by spraying the specimen surfaces with an aqueous solution of poly(vinyl alcohol) before each sectioning stroke. The spray freezes upon the surface and forms a tough coating which facilitates subsequent sectioning and handling especially of difficult material. The sections are affixed upon cold glass slides covered with an improved formulation of pressure-sensitive adhesive. During further processing of the affixed sections, the PVA-coating and any surrounding supporting medium dissolve without traces in the first aqueous incubation or staining solution.
Fink
S
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Institut für Forstbotanik und Baumphysiologie, Albert-Ludwigs-Universität, Freiburg im Breisgau, Federal Republic of Germany.
D000818:Animals; D002417:Cattle; D016616:Cryoultramicrotomy / Q000295:instrumentation / Q000379:methods*; D014422:Turkeys