title: Automated microanalysis of creatinine by coupled enzyme reactions.
abstract: Hydrogen peroxide was generated from creatinine by the sequential enzyme reactions of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. Hydrogen peroxide was, in turn, used stoichiometrically for the condensation of 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine catalyzed by horse-radish peroxidase, resulting in the formation of quinone dye with maximum absorbance at 546 nm. The optimized assay conditions for the enzymatic determination of creatinine in a HITACHI 7250 autoanalyzer was established. This system, which requires less than 5 microliters of sample, was found to be the most economical for laboratories equipped with autoanalyzers.
authors.affiliation: Central Research Laboratories, Kobayashi Pharmaceutical Co., Ltd., Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Asano
authors.forename: H
authors.initials: H
authors.lastname: Iwasaki
authors.forename: K
authors.initials: K
authors.lastname: Fujimura
authors.forename: M
authors.initials: M
authors.lastname: Ikeda
authors.forename: Y
authors.initials: Y
authors.lastname: Sugimoto
authors.forename: A
authors.initials: A
authors.lastname: Matsubara
authors.forename: K
authors.initials: K
authors.lastname: Yano
authors.forename: H
authors.initials: H
authors.lastname: Irisawa
authors.forename: F
authors.initials: F
authors.lastname: Kono
authors.forename: M
authors.initials: M
authors.lastname: Kanbe
mesh_terms: D001322:Autoanalysis / Q000379:methods*; D004796:Clinical Enzyme Tests / Q000379:methods*; D003404:Creatinine / Q000097:blood*; D006801:Humans
chemical_list: D003404:Creatinine