Evidence for two cDNA clones encoding human GM2-activator protein.
Two cDNAs encoding GM2 activator, pGM2A (648 bp) and GAP (1093 bp), were isolated from human placenta lambda gt11 libraries. The DNA sequence of pGM2A from 1 to 302 was almost identical with GAP, but diverged from 303-648. PCR was used to demonstrate the presence of both species of GM2 activator in placental RNA. Both cDNAs hybridized to mRNAs of approximately 2.3 kb and to identical single bands on genomic Southern blots.
Nagarajan
S
S
Department of Biochemistry, Tulane University School of Medicine, New Orleans, LA 70112.
Chen
H C
HC
Li
S C
SC
Li
Y T
YT
Lockyer
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005347:Fibroblasts / Q000737:chemistry / Q000502:physiology; D049289:G(M2) Activator Protein; D005784:Gene Amplification; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D010920:Placenta / Q000737:chemistry / Q000502:physiology; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
D049289:G(M2) Activator Protein; D010446:Peptide Fragments; D011506:Proteins; D012333:RNA, Messenger; D004247:DNA