title: Release of anti-PAF activity from PAF-binding human ovarian cancer cells in culture.
abstract: The human ovarian cancer cell line EFO-27 in culture spontaneously produced anti-PAF activity, which eluted from HPLC in the range of synthetic PAF. The activity therefore appears to be due to an antagonistic PAF-analogue. It was detected by a suppressed PAF-induced platelet aggregation in vitro. EFO-27 cells were found to be able to bind synthetic PAF with saturable binding kinetics. This binding led to reduced cell proliferation. The production of anti-PAF activity by EFO-27 cells resembles an autocrine growth regulation in the light of recent findings that other malignant transformed cell lines produce PAF-like activity in vitro if stimulated appropriately.
authors.affiliation: University Women's Hospital, University of Tübingen, Germany.
authors.forename: L M
authors.initials: LM
authors.lastname: Adamson
authors.forename: V
authors.initials: V
authors.lastname: Hanf
authors.forename: S G
authors.initials: SG
authors.lastname: Mittman
authors.forename: G
authors.initials: G
authors.lastname: Siebert
authors.forename: H
authors.initials: H
authors.lastname: Seeger
authors.forename: W E
authors.initials: WE
authors.lastname: Simon
authors.forename: H R
authors.initials: HR
authors.lastname: Tinneberg
mesh_terms: D002455:Cell Division / Q000187:drug effects; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D014407:Tumor Cells, Cultured
chemical_list: D010972:Platelet Activating Factor