Possible indices for the detection of the administration of dihydrotestosterone to athletes.
Dihydrotestosterone (DHT) can be used by an athlete as an anabolic steroid to evade the current International Olympic Committee approved drug tests. To investigate the possibility of a method for its detection, the heptanoate ester of DHT was administered to two male subjects (150 mg i.m.). Urine samples, collected before and after the injection, were subjected to enzymatic hydrolysis and the excretion rates of DHT, 5 alpha-androstane-3 alpha,17 beta-diol (3 alpha-diol) and testosterone (T) were determined by radioimmunoassay. Relative changes in the excretion of DHT, 3 alpha-diol, 5 alpha-androstane-3 beta,17 beta-diol (3 beta-diol), 5 beta-androstane-3 alpha, 17 beta-diol (5 beta-diol), T and epitestosterone (17 alpha hydroxyandrost-4-en-3-one; Epi-T) were determined by gas chromatography-mass spectrometry (GC-MS). Following administration of DHT, the urinary excretion rates of DHT, 3 alpha-diol and 3 beta-diol increased when compared to those of T, Epi-T, 5 beta-diol and luteinizing hormone (LH). Concentrations of DHT in the plasma increased whereas those of T, LH and follicle stimulating hormone decreased. The changes following such modest doses of DHT suggest that these ratios of urinary hormones may be used for the detection of doping with DHT.
Southan
G J
GJ
Department of Chemical Pathology, United Medical School, Guys Hospital, London, England.
Brooks
R V
RV
Cowan
D A
DA
Kicman
A T
AT
Unnadkat
N
N
Walker
C J
CJ
D000328:Adult; D000368:Aged; D015113:Androstane-3,17-diol / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D013196:Dihydrotestosterone / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007986:Luteinizing Hormone / Q000652:urine; D008297:Male; D011863:Radioimmunoassay; D013177:Sports; D013739:Testosterone / Q000652:urine
D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol; D013739:Testosterone; D007986:Luteinizing Hormone