[The characteristics of the action of gamma irradiation on the secondary structure of ribonuclease in vitro and the effect of oxygen on this process].
The change in the secondary structure of ribonuclease after 60Co gamma irradiation with a dose of 1,000 Gy in 0.2% aqueous solution was estimated using the circular dichroism method. The beta structures were significantly changed, while other types of the secondary structure (alpha-helix and beta-turns) changed insignificantly. The secondary structure injury was also affected by oxygen. The data are attributed to characteristics of the secondary structure of this enzyme.
Kharchenko
L I
LI
Pavlovskaia
T E
TE
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D005720:Gamma Rays; D010100:Oxygen / Q000494:pharmacology*; D010179:Pancreas / Q000201:enzymology; D011487:Protein Conformation / Q000187:drug effects / Q000528:radiation effects; D012260:Ribonucleases / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects*; D012996:Solutions; D014618:Vacuum
D012996:Solutions; D012260:Ribonucleases; D010100:Oxygen
Osobennosti deĭstviia gamma-oblucheniia na vtorichnuiu strukturu ribonukleazy in vitro i vliianie kisloroda na étot protsess.