Release of anti-PAF activity from PAF-binding human ovarian cancer cells in culture.
The human ovarian cancer cell line EFO-27 in culture spontaneously produced anti-PAF activity, which eluted from HPLC in the range of synthetic PAF. The activity therefore appears to be due to an antagonistic PAF-analogue. It was detected by a suppressed PAF-induced platelet aggregation in vitro. EFO-27 cells were found to be able to bind synthetic PAF with saturable binding kinetics. This binding led to reduced cell proliferation. The production of anti-PAF activity by EFO-27 cells resembles an autocrine growth regulation in the light of recent findings that other malignant transformed cell lines produce PAF-like activity in vitro if stimulated appropriately.
Adamson
L M
LM
University Women's Hospital, University of Tübingen, Germany.
Hanf
V
V
Mittman
S G
SG
Siebert
G
G
Seeger
H
H
Simon
W E
WE
Tinneberg
H R
HR
D002455:Cell Division / Q000187:drug effects; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D014407:Tumor Cells, Cultured
D010972:Platelet Activating Factor