Comparison of immunotyping of Chlamydia trachomatis by indirect fluorescent-antibody staining and radioimmunoassay.
Chlamydia trachomatis isolates from 58 patients were immunotyped by radioimmunoassay (RIA) and fluorescent-antibody staining (FA) by using monoclonal antibodies. Unambiguous typing was observed with 55 isolates by FA and 53 isolates by RIA, although 3 isolates were typed by RIA but not FA. FA required less antigen and was more sensitive in detecting mixed infections.
van der Pol
B J
BJ
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202.
Jones
R B
RB
D000911:Antibodies, Monoclonal; D015373:Bacterial Typing Techniques / Q000706:statistics & numerical data; D002692:Chlamydia trachomatis / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D011863:Radioimmunoassay; D012680:Sensitivity and Specificity; D012703:Serotyping
D000911:Antibodies, Monoclonal