Immunohistochemical demonstration of a new thiamine diphosphate-binding protein in the rat digestive tract.
We purified a new thiamine diphosphate-binding protein (ThDP-BP), produced an antiserum against it, and examined its immunohistochemical distribution in the rat gastrointestinal tract using the avidin-biotin complex method. Positive staining for ThDP-BP was found in the epithelial glands of the stomach, small intestine and large intestine, and in the nuclei of hepatocytes. Measurement of the content of thiamine and its phosphate esters in extracts from the gastric and intestinal mucosa also indicated the presence of ThDP-BP in the stomach and intestinal mucosa. ThDP-BP may be useful for investigating the absorption and metabolism of the thiamine in metabolic disorders.
Yoshioka
H
H
Department of Geriatric Medicine, Kyoto University, Japan.
Nishino
K
K
Usui
T
T
Sung
M
M
Ohshio
G
G
Sugiyama
T
T
Kita
T
T
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004848:Epithelium / Q000737:chemistry / Q000648:ultrastructure; D005753:Gastric Mucosa / Q000737:chemistry*; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000648:ultrastructure; D007420:Intestine, Large / Q000737:chemistry / Q000648:ultrastructure; D007421:Intestine, Small / Q000737:chemistry / Q000648:ultrastructure; D008099:Liver / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013831:Thiamine / Q000032:analysis; D013835:Thiamine Pyrophosphate / Q000032:analysis / Q000378:metabolism*; D013838:Thiamine Triphosphate / Q000032:analysis
D002352:Carrier Proteins; D013838:Thiamine Triphosphate; D013835:Thiamine Pyrophosphate; D013831:Thiamine