"On-line internal surface reversed-phase cleaning": the direct HPLC analysis of crude biological samples. Application to the kinetics of degradation of oligonucleotides in cell culture medium.
An "on-line" HPLC analysis of crude biological samples is described. A precolumn of internal surface reversed-phase material allows the passage of proteins and other unwanted products while retaining analytes which are transferred, concentrated and chromatographed on a conventional reverse-phase or ion-exchange HPLC column. This protocol allows precise kinetics of the degradation of an oligonucleotide in cell culture to be obtained without radiolabeling or sample preparation.
Pompon
A
A
Laboratoire de Chimie Bio-Organique, U.R.A. CNRS No. 488, Université Montpellier II, France.
Lefebvre
I
I
Imbach
J L
JL
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D007700:Kinetics; D009841:Oligonucleotides / Q000737:chemistry*
D003470:Culture Media; D009841:Oligonucleotides