title: Estrogen and phenol sulfotransferase activities in human fetal lung.
abstract: The sulfation of steroid hormones and xenobiotics by human fetal lung cytosol was examined. 1-Naphthol and estrone were extensively sulfated, whereas paracetamol and dehydroepiandrosterone were not good substrates for the pulmonary enzyme. Investigation of the thermostability and inhibition by 2,6-dichloro-4-nitrophenol (DCNP) of the 1-naphthol and estrone sulfotransferase (ST) activities revealed that the estrone ST activity was more thermolabile and more readily inhibited by DCNP than was the 1-naphthol ST activity. Anion exchange chromatography by FPLC resulted in the resolution of two 1-naphthol ST activities, with the estrone ST activity co-eluting with the more basic 1-naphthol ST activity. When human fetal lung cytosol was subjected to gel filtration FPLC, both the 1-naphthol and estrone ST activities had the same native molecular weight of 63,000 Da. this is the first demonstration of estrogen ST activity in human fetal lung. These results suggest that there are at least two forms of sulfotransferase in human fetal lung and that this tissue is capable of sulfating both xenobiotics and endogenous compounds.
authors.affiliation: Department of Biochemical Medicine, University of Dundee, Ninewells Hospital and Medical School, U.K.
authors.forename: A L
authors.initials: AL
authors.lastname: Jones
authors.forename: R
authors.initials: R
authors.lastname: Hume
authors.forename: K J
authors.initials: KJ
authors.lastname: Bamforth
authors.forename: M W
authors.initials: MW
authors.lastname: Coughtrie
mesh_terms: D015239:Arylsulfotransferase / Q000378:metabolism*; D002853:Chromatography, Liquid; D003600:Cytosol / Q000737:chemistry; D004795:Enzyme Stability; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000201:enzymology / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D008168:Lung / Q000196:embryology* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009596:Nitrophenols
chemical_list: D004967:Estrogens; D009596:Nitrophenols; C015802:2,6-dichloro-4-nitrophenol; D014453:Glucuronosyltransferase; D015239:Arylsulfotransferase