Formation of stable p53 homotetramers and multiples of tetramers.
We have used gradient gel electrophoresis and chemical cross-linking to analyze the quaternary structure of purified, wild-type, murine p53. Under nondenaturing conditions, p53 electrophoreses as tetramers and multiples of tetramers. Under denaturing conditions, fully cross-linked p53 also behaves as tetrameric structures. We confirmed the composition of the tetramers by partially cross-linking p53 and dissociating tetramers into monomers, dimers, and trimers.
Stenger
J E
JE
Department of Microbiology, State University of New York, Stony Brook 11794.
Mayr
G A
GA
Mann
K
K
Tegtmeyer
P
P
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008970:Molecular Weight; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D016159:Tumor Suppressor Protein p53