title: Protein and antigen variability among strains of Mycoplasma arthritidis.
abstract: Six strains of Mycoplasma arthritidis isolated from different host tissues were examined for differences in their proteins and antigens by using one- and two-dimensional electrophoretic techniques as well as immunoblotting. One-dimensional electrophoresis revealed differences in concentrations of individual bands, but not differences in the overall banding pattern. By two-dimensional electrophoretic analysis, 25 proteins were identified as strain-variable whereas the majority of protein spots was strain-constant (about 195 after IEF-2D-PAGE and 145 after NEPHGE-2D-PAGE). Immunoblot analysis using an antiserum against the type-strain of Mycoplasma arthritidis (PG 6) revealed size-heterogeneity of antigens of all six strains. An epitopic relationship between these size-variant antigens could be demonstrated by using monospecific antibodies produced against some of these antigens of Mycoplasma arthritidis. Furthermore, we describe a highly variable antigen of Mycoplasma arthritidis similar to that shown previously in Mycoplasma pulmonis.
authors.affiliation: Department of Microbiology, University of Alabama School of Medicine, Birmingham 35294.
authors.forename: C T
authors.initials: CT
authors.lastname: Stadtlander
authors.forename: H L
authors.initials: HL
authors.lastname: Watson
mesh_terms: D000818:Animals; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000032:analysis*; D001170:Arthritis, Infectious / Q000382:microbiology / Q000662:veterinary; D001426:Bacterial Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D009174:Mycoplasma / Q000737:chemistry* / Q000276:immunology; D051381:Rats; D012376:Rodent Diseases / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins