title: Altered neutrophil function following reperfusion of an ischemic myocutaneous flap.
abstract: The neutrophil has been implicated as a source of oxygen free radicals provoking the reperfusion injury in various ischemic organs. This provided the motivation to explore the pathophysiologic role of the neutrophil in a swine model of postischemic latissimus dorsi myocutaneous flaps. Neutrophil function, neutrophil sequestration, and the anatomic distribution of muscle injury were estimated following a 6- to 8-hour global ischemic insult. Neutrophil function as measured by phorbol myristate acetate-stimulated superoxide production was found to be enhanced on reperfusion of ischemic flaps (n = 17). Neutrophil sequestration estimated from the arterial-venous difference of flap blood (n = 12) demonstrated that postischemic flaps more avidly sequester neutrophils than nonischemic flaps. The anatomic distribution of muscle injury (n = 7) was predominantly localized to the proximal portion of the ischemic flap. The enhanced functional response exhibited by neutrophils reperfusing an ischemic myocutaneous flap supports an active neutrophil role in the mediation of reperfusion injury.
authors.affiliation: Microsurgical Research Laboratories, Royal Victoria Hospital, Montreal, P.Q., Canada.
authors.forename: C
authors.initials: C
authors.lastname: Lee
authors.forename: C L
authors.initials: CL
authors.lastname: Kerrigan
authors.forename: J M
authors.initials: JM
authors.lastname: Tellado
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D005260:Female; D009132:Muscles / Q000098:blood supply; D009247:NADH, NADPH Oxidoreductases / Q000097:blood; D019255:NADPH Oxidases; D009504:Neutrophils / Q000201:enzymology*; D015427:Reperfusion Injury / Q000201:enzymology / Q000209:etiology*; D012867:Skin / Q000098:blood supply; D013524:Surgical Flaps / Q000502:physiology*; D016019:Survival Analysis; D013552:Swine
chemical_list: D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases