Rapid surveying of DNA sequence variation in natural populations.
DNA sequencing can be costly and time consuming for population studies because of the relative rarity of variation along exons. These problems can be substantially reduced by the use of the polymerase chain reaction on introns using primers from the exon region. These problems can be further reduced by the use of denaturing gradient gel electrophoresis to identify those alleles in need of sequencing.
Lessa
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Museum of Vertebrate Zoology, University of California, Berkeley 94720.
D000818:Animals; D001483:Base Sequence; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D005821:Genetic Techniques; D014644:Genetic Variation; D005914:Globins / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012377:Rodentia; D012689:Sequence Homology, Nucleic Acid
D005914:Globins; D004247:DNA