title: The assessment of episodic metal pollution. I. Uses and limitations of tissue contaminant analysis in rainbow trout (Oncorhynchus mykiss) after short waterborne exposure to cadmium or copper.
abstract: Rainbow trout were exposed to 8 h episodes of either Cd (0.1 or 0.2 mg/L) or Cu (0.1 or 0.6 mg/L) at pH 7. Gill, liver, mucus, blood, skin, muscle, and body remainder were examined for metal content during and after exposure. Accumulation of Cd occurred mostly in the gill, liver, mucus, and muscle, with the gill supporting 21-36% of the body burden. Copper appeared primarily in the gill, liver and mucus, with the gill supporting 4-40% of the body burden. After exposure, rapid clearance of toxicant from the gill and mucus occurred within 16 h, while contamination in the liver increased, resulting in the liver supporting 11-14% or 58-61% of the Cd or Cu body burdens respectively 24 h after exposure. The time of sampling after exposure as well as choice of organs and control data are important aspects of post-exposure analysis. In the absence of control data, exposure status may be derived from the ratio of contaminants in the gill/liver and the distribution of the body burden.
authors.affiliation: Department of Biological Sciences, The University, Dundee, Scotland, United Kingdom.
authors.forename: R D
authors.initials: RD
authors.lastname: Handy
mesh_terms: D000818:Animals; D001822:Body Burden; D002104:Cadmium / Q000493:pharmacokinetics*; D003300:Copper / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014337:Trout / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics*
chemical_list: D014874:Water Pollutants, Chemical; D002104:Cadmium; D003300:Copper