title: The presence of phospholipase A2 in bovine adrenal medulla: arguments for more than one type of phospholipase A2.
abstract: Since phospholipase A2 (PLA2) is expected to play a role in the mechanism of exocytosis, the presence and subcellular localization of PLA2 in bovine adrenal medulla have been studied. The results of this study reveal that, although a large part of the PLA2 activity in chromaffin cells is due to a lysosomal PLA2, a cytoplasmic PLA2 is also present. This finding is supported by experiments in which the influence of pH, CaCl2 and NaCl on cytoplasmic PLA2 as well as the binding capacity to concanavalin A are investigated. According to these results the properties of a cytoplasmic PLA2 are clearly different from those reported by other authors for the lysosomal PLA2. For this reason, in chromaffin cells a PLA2 could be present which remains in the cytosol when the cell is in rest. Future experiments will have to prove whether this PLA2 becomes associated with the plasma membrane upon stimulation of the cell, thus mediating exocytosis.
authors.affiliation: Department of Medicine, University of Antwerp, Wilrijk, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Petit
authors.forename: B
authors.initials: B
authors.lastname: Miserez
authors.forename: J
authors.initials: J
authors.lastname: De Block
authors.forename: G
authors.initials: G
authors.lastname: Van Dessel
authors.forename: W
authors.initials: W
authors.lastname: De Potter
mesh_terms: D000313:Adrenal Medulla / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D002500:Centrifugation, Isopycnic; D003600:Cytosol / Q000201:enzymology; D004533:Egtazic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000378:metabolism; D010741:Phospholipases A / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D054467:Phospholipases A2; D013347:Subcellular Fractions / Q000201:enzymology; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D004533:Egtazic Acid; D010741:Phospholipases A; D008071:Lipoprotein Lipase; D054467:Phospholipases A2; D010738:Type C Phospholipases; D002118:Calcium