A new human interferon-gamma mutant harboring EGF receptor-interfering sequence possesses high antiproliferative activity.
An interferon gamma (IFN-gamma) mutant gene harboring an additional epidermal growth factor (EGF) sequence at its 3' terminal was constructed and highly expressed in E. coli by using pBV220 as a vector. The new fusion protein-IFN-gamma-EGF possesses not only high antiviral activity, but also high antiproliferative effect as demonstrated by 3H-TdR incorporation method using CN II cells which are rich in EGF receptors.
Wang
X M
XM
National Laboratory of Molecular Virology and Genetic Engineering, Chinese Academy of Preventive Medicine, Beijing.
Li
Y Y
YY
Jin
Q
Q
Zhang
Z Q
ZQ
Hou
Y D
YD
D000595:Amino Acid Sequence; D002455:Cell Division / Q000187:drug effects; D004274:DNA, Recombinant; D066246:ErbB Receptors / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects; D007371:Interferon-gamma / Q000145:classification / Q000235:genetics* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010957:Plasmids; D015202:Protein Engineering
D004274:DNA, Recombinant; D007371:Interferon-gamma; D066246:ErbB Receptors