Simulation of the time course of macromolecular separations in an ultracentrifuge. I. Formation of a cesium chloride density gradient at 25 degrees C.
A method is described for rapid numerical simulation of the time course of the formation of a density gradient of CsCl in an ultracentrifuge at 25 degrees C. Results of simulations compare well with those of experiments carried out in analytical and preparative ultracentrifuges.
Minton
A P
AP
Laboratory of Biochemical Pharmacology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
D002499:Centrifugation, Density Gradient; D002586:Cesium / Q000737:chemistry*; D002712:Chlorides; D007700:Kinetics; D008433:Mathematics; D013696:Temperature
D002712:Chlorides; D002586:Cesium; C028019:cesium chloride