An animal model of Kaposi's sarcoma. I. Immune status of CD1 mice undergoing dimethyl hydrazine treatment to induce angiosarcomas and other malignancies.
Dimethylhydrazine (DMH) induces colonic cancer and angiosarcomas in mice. In order to determine pertinence of mouse angiosarcoma as a model to AIDS associated Kaposi's sarcoma (KS), we investigated if immune dysfunction occurred during tumor development by DMH. Outbred CD1 male mice received once weekly DMH a 20 mg/kg body weight dose s.c. for 33 weeks. Every two weeks initially and then every week groups of DMH-treated and control animals were sacrificed to determine a) peripheral blood and splenic T cell subset ratio b) 4-day plaque forming cell (PFC) response to i.p. sheep red blood cells (SRBC) and c) mitogenic response of spleen cells to Concanavalin A (Con A) and lipopolysaccharide (LPS). No change in T helper/T suppressor + cytotoxic T cell (Th/Tsupp. + CTL) and mitogenic response to spleen cells to Con A was noted whereas PFC response of animals to SRBC and mitogenic response of spleen cells to LPS decreased. These data suggest that either infection with T cell depleting virus such as LP:BM5 or immunosuppressive drugs affecting T cell function, such as steroids may be required to bring the immune status of DMH treated animals closer to that of AIDS associated KS bearing human subjects.
Dubé
M
M
Departement d'Anatomie et Biologie Cellulaire, Faculté de Médécine, Université de Sherbrooke, Québec, Canada.
Madarnas
P
P
Rola-Pleszczynski
M
M
Nigam
V N
VN
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004127:Dimethylhydrazines; D004195:Disease Models, Animal; D000518:Eflornithine / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D006394:Hemangiosarcoma / Q000139:chemically induced / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D012514:Sarcoma, Kaposi / Q000209:etiology* / Q000276:immunology; D012756:Sheep; D013154:Spleen / Q000276:immunology / Q000473:pathology
D004127:Dimethylhydrazines; D000518:Eflornithine