The IRG-47 gene is IFN-gamma induced in B cells and encodes a protein with GTP-binding motifs.
The murine pre-B cell line 70Z/3 has been studied extensively as a model system for B cell differentiation induced by cytokines and mitogens. IFN-gamma treatment of 70Z/3 pre-B cells activates Ig kappa L chain gene expression, resulting in the appearance of surface IgM characteristic of B cells. We have isolated and characterized a new cDNA clone, called IRG-47, whose mRNA is transiently elevated up to 30-fold at 4 to 6 h after IFN-gamma induction of 70Z/3 pre-B cells. The IFN-gamma induction of IRG-47 mRNA occurs in all pre-B and in most B lineage cell lines examined but is only induced in three cell lines tested from other lineages. Activation of IRG-47 gene transcription appears to account for the rapid increase in IRG-47 mRNA after induction. Transcription of the IRG-47 gene is down-regulated 10 h postinduction at a time when the level of IRG-47 mRNA is also declining. The complete sequence of the IRG-47 cDNA clone is predicted to encode a 47-KDa protein which, although unique in its sequence, bears resemblance to a number of nucleotide-binding proteins. The IRG-47 coding sequence contains all three highly conserved peptide motifs in the appropriate spacing characteristic of guanine nucleotide-binding proteins.
Gilly
M
M
Molecular Biology Institute, UCLA School of Medicine 90024.
Wall
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D019204:GTP-Binding Proteins / Q000235:genetics*; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
D012333:RNA, Messenger; D007371:Interferon-gamma; D004247:DNA; D019204:GTP-Binding Proteins