title: A new human interferon-gamma mutant harboring EGF receptor-interfering sequence possesses high antiproliferative activity.
abstract: An interferon gamma (IFN-gamma) mutant gene harboring an additional epidermal growth factor (EGF) sequence at its 3' terminal was constructed and highly expressed in E. coli by using pBV220 as a vector. The new fusion protein-IFN-gamma-EGF possesses not only high antiviral activity, but also high antiproliferative effect as demonstrated by 3H-TdR incorporation method using CN II cells which are rich in EGF receptors.
authors.affiliation: National Laboratory of Molecular Virology and Genetic Engineering, Chinese Academy of Preventive Medicine, Beijing.
authors.forename: X M
authors.initials: XM
authors.lastname: Wang
authors.forename: Y Y
authors.initials: YY
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Jin
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Y D
authors.initials: YD
authors.lastname: Hou
mesh_terms: D000595:Amino Acid Sequence; D002455:Cell Division / Q000187:drug effects; D004274:DNA, Recombinant; D066246:ErbB Receptors / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects; D007371:Interferon-gamma / Q000145:classification / Q000235:genetics* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010957:Plasmids; D015202:Protein Engineering
chemical_list: D004274:DNA, Recombinant; D007371:Interferon-gamma; D066246:ErbB Receptors