Cytogenetic comparison of two poorly differentiated human lung squamous cell carcinoma lines.
This paper presents a cytogenetic analysis of two established but early-passage (passages 5 and 18) cell lines derived from histologically similar, poorly differentiated lymph node metastases of squamous cell carcinoma of the lung. The cell lines showed 3 shared marker chromosomes, del(1)(q11), del(2)(p11.1) and del(2)(q11.1). One of the lines (DLKP) had 8 additional markers including structural rearrangements such as translocations and isochromosomes. Five additional markers (including two deletions of chromosome 3) were found in DLRP. A notable feature of DLRP was the high incidence of telomeric association evident in the majority of metaphase plates. Over-representation of chromosome 7 was a characteristic feature of metaphases derived from DLKP, and identification of i(21q) in this cell line was an unusual finding. The results indicate significant cytogenetic heterogeneity between these early-passage cell lines derived from two apparently histologically similar tumors.
Law
E
E
National Cell and Tissue Culture Centre/Bioresearch Ireland, Dublin.
Gilvarry
U
U
Lynch
V
V
Gregory
B
B
Grant
G
G
Clynes
M
M
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary; D002872:Chromosome Deletion; D002873:Chromosome Fragility; D002878:Chromosomes, Human, Pair 1; D002889:Chromosomes, Human, Pair 2; D002893:Chromosomes, Human, Pair 3; D002897:Chromosomes, Human, Pair 7; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics* / Q000556:secondary; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D011123:Polyploidy; D016615:Telomere; D014407:Tumor Cells, Cultured
D005819:Genetic Markers