title: Characterization of an 8.7-kilobase thiostrepton resistance-encoding plasmid (pGIF3) of Streptomyces incarnatus.
abstract: The low-copy-number 8.7-kb plasmid pGIF3 of Streptomyces incarnatus was studied after cloning in the Escherichia coli vector pBR322; a restriction map was constructed. Southern blot analysis showed that pGIF3 in S. incarnatus occurs predominantly as integrated in a larger replicon. The plasmid carries a gene for thiostrepton resistance having no homology with the known thiostrepton resistance gene from Streptomyces azureus.
authors.affiliation: Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
authors.forename: H
authors.initials: H
authors.lastname: Malina
authors.forename: M
authors.initials: M
authors.lastname: Robert-Gero
mesh_terms: D015139:Blotting, Southern; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000648:ultrastructure; D004278:DNA, Superhelical / Q000648:ultrastructure; D004352:Drug Resistance, Microbial / Q000235:genetics; D011815:R Factors; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D013883:Thiostrepton / Q000494:pharmacology*
chemical_list: D004269:DNA, Bacterial; D004278:DNA, Superhelical; D013883:Thiostrepton