title: Influence of ischemia on blood-brain and blood-CSF calcium transport.
abstract: The objective was to explore whether the increased incorporation of 45Ca into selectively vulnerable neurons, observed after transient ischemia, can be explained by enhanced blood-tissue and/or enhanced blood-CSF transfer. Anesthetized rats were subjected to 15 min of forebrain ischemia, with recirculation for 20 or 60 min, or 24 h. The transfer constants (Kin) and unidirectional fluxes (Jin) for calcium in tissue and CSF were determined following i.v. injection of 45Ca, integration of the curve for plasma-specific activity over 10 min, and sampling of cisternal CSF, and tissue (cortex, caudoputamen, hippocampus, and cerebellum). Predictably, values for Kin and Jin in control animals were much larger in CSF than in tissues, and hippocampus had higher values than the other areas, probably because of its closeness to the lateral ventricle. Ischemia failed to alter the Kin and Jin values, demonstrating that the low permeability of blood-brain and blood-CSF barriers to calcium is upheld both in the immediate recirculation period, and after 24 h of recirculation. The results support the contention that the increased 45Ca incorporation during recovery is due to increased calcium cycling across functionally altered cell membranes.
authors.affiliation: Laboratory for Experimental Brain Research, University of Lund, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Ohta
authors.forename: G
authors.initials: G
authors.lastname: Gidö
authors.forename: B K
authors.initials: BK
authors.lastname: Siesjö
mesh_terms: D000818:Animals; D001692:Biological Transport; D001812:Blood-Brain Barrier; D002545:Brain Ischemia / Q000097:blood / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism* / Q000493:pharmacokinetics; D002132:Calcium Radioisotopes; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D004195:Disease Models, Animal; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D015424:Reperfusion
chemical_list: D002132:Calcium Radioisotopes; D002118:Calcium