title: [Effect of serum factors on the differentiation of a colon cancer cell line Caco-2].
abstract: A human colon cancer cell line Caco-2 undergoes spontaneous enterocytic differentiation under the standard culture condition. We examined the changes in the proliferative activity of Caco-2 cells during the differentiation, and the effect of serum factors on this spontaneous differentiation. Caco-2 cells cultured in FCS-containing media exhibited the differentiation phenotypes such as dome formation and increase of ALP activity after the 6th day of culture. Cell cycle analysis showed a marked decrease in S phase cell population during the cell differentiation. When Caco-2 cells were cultured in FCS free media, the cell population of S phase remained unchanged during culture period and the differentiation phenotypes were not induced. The addition of FCS to Caco-2 cell cultured in the absence of FCS resulted in the rapid induction of the differentiation phenotypes. These results suggest that differentiation of Caco-2 cells is closely related to the reduction of cell proliferation and requires some factors in FCS.
authors.affiliation: Department of Internal Medicine, School of Medicine, Keio University.
authors.forename: A
authors.initials: A
authors.lastname: Hara
authors.forename: T
authors.initials: T
authors.lastname: Hibi
authors.forename: M
authors.initials: M
authors.lastname: Yoshioka
authors.forename: K
authors.initials: K
authors.lastname: Toda
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Morita
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Serizawa
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Iwao
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000097:blood / Q000494:pharmacology*; D002417:Cattle; D002454:Cell Differentiation; D002455:Cell Division; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D014407:Tumor Cells, Cultured / Q000473:pathology
chemical_list: D001685:Biological Factors