GM1 ganglioside effects on astroglial response in the rat nucleus basalis magnocellularis and its cortical projection areas after electrolytic or ibotenic lesions.
We examined the effects of chronic GM1 ganglioside injections on the astroglial response to bilateral electrolytic or ibotenic acid lesions in the nucleus basalis magnocellularis (NBM) within the NBM and in three cortical projection areas of NBM neurons. Glial fibrillary acidic protein (GFAP) immunohistochemistry was used to visualize the reactive astrocytes. Twenty-six days after injury, extensive astrogliosis was observed within the NBM after both types of lesions. An increased number of GFAP-positive cells were found in the cortex of saline-treated rats following electrolytic but not ibotenic lesions. We suggest that the loss of fibers of passage within the lesion area may account for the difference in cortical gliosis following the two types of damage. Although 17 days of GM1 injections did not affect astrocyte morphology within the NBM, ganglioside treatment reduced the number of GFAP-positive cells after electrolytic but not after ibotenic lesions. Within the cortex, a decrease in GFAP immunoreactivity, size, and number of astrocytes was only observed after electrolytic lesion. These data indicate that a decrease in the astroglial response to injury is the result of an interaction between the type of injury (electrolytic lesion) and chronic GM1 treatment.
Lescaudron
L
L
Institute of Animal Behavior, Rutgers University, State University of New Jersey, Newark 07102.
Bitran
B S
BS
Stein
D G
DG
D000704:Analysis of Variance; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D004558:Electric Stimulation; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007051:Ibotenic Acid / Q000633:toxicity*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D014726:Vestibular Nuclei / Q000187:drug effects / Q000473:pathology / Q000502:physiology*
D005904:Glial Fibrillary Acidic Protein; D007051:Ibotenic Acid; D005677:G(M1) Ganglioside