title: Identification of a novel secreted glycoprotein of the yeast Saccharomyces cerevisiae stimulated by heat shock.
abstract: A new secreted yeast glycoprotein with an Mr of about 400 kDa (gp400) has been found. The glycoprotein is an O-mannosylated oligomer, whose synthesis and export into culture medium are stimulated by heat shock. Intracellular transport of gp400 is carried out by membrane vesicles distinct from the known constitutive secretory vesicles. Immunological analysis revealed gp400 only in Saccharomyces species.
authors.affiliation: Institute of Biochemistry and Physiology of Microorganisms, U.S.S.R.
authors.forename: V V
authors.initials: VV
authors.lastname: Lupashin
authors.forename: S V
authors.initials: SV
authors.lastname: Kononova
authors.lastname: Ratner YeN
authors.forename: A B
authors.initials: AB
authors.lastname: Tsiomenko
authors.forename: I S
authors.initials: IS
authors.lastname: Kulaev
mesh_terms: D000596:Amino Acids / Q000032:analysis; D003594:Cytoplasmic Granules / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006031:Glycosylation; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006358:Hot Temperature; D008854:Microscopy, Electron; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000596:Amino Acids; D005656:Fungal Proteins; D006023:Glycoproteins; D006360:Heat-Shock Proteins