Lens fiber cell differentiation and expression of crystallins in co-cultures of human fetal lens epithelial cells and fibroblasts.
Growth of the ocular lens is directed by the division and differentiation of a single layer of epithelial cells located at the equatorial region. It is conceivable that this region of the lens capsule presents a special microenvironment modulated by molecular cues emanating from the surrounding tissues. In an effort to investigate the source and nature of these molecular cues, we co-cultured human fetal lens epithelial cells and fibroblasts derived from the ciliary body. We observed morphological differentiation as evidenced by the appearance of differentiating lentoid structures associated with fibroblasts. Characterization of the expression of lens-specific proteins revealed that in addition to alpha B-crystallin, these lentoid structures contain the lens fiber cell-specific proteins, alpha A-crystallin, beta B2-crystallin and gamma S-crystallin. None of these crystallins could be found in the surrounding undifferentiated lens epithelial cells. Interestingly, alpha B-crystallin usually present in lens epithelial cells when cultured alone, was found to be markedly decreased, both in synthesis and content in the cells surrounding the differentiated structures, suggesting that the process of differentiation in vitro may concomitantly produce a factor(s) which modulates alpha B-crystallin expression in these cells.
Nagineni
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Jules Stein Eye Institute, University of California, School of Medicine, Los Angeles 90024-1771.
Bhat
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SP
D002454:Cell Differentiation; D002478:Cells, Cultured; D003459:Crystallins / Q000096:biosynthesis*; D004847:Epithelial Cells; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology* / Q000378:metabolism
D003459:Crystallins