title: Sensitive amplified immunoenzymometric assays (IEMA) for human insulin and intact proinsulin.
abstract: Immunoenzymometric assays (IEMAs) for human insulin and intact proinsulin were developed using the amplification system developed by Johannsson et al. (Clin. Chim. Acta 148 (1985) 119-124) for the detection of the enzyme alkaline phosphatase. The detection limit of the assays was 0.8 pmol/l for proinsulin and 0.8 pmol/l for insulin whereas it was 1.8 pmol/l and 2.3 pmol/l respectively for the homologous immunoradiometric assays (IRMA). These assays are superior to immunoradiometric assays in terms of sensitivity, shelf-life of the labelled antibody and suitability for automation.
authors.affiliation: Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, UK.
authors.forename: B
authors.initials: B
authors.lastname: Alpha
authors.forename: L
authors.initials: L
authors.lastname: Cox
authors.forename: N
authors.initials: N
authors.lastname: Crowther
authors.forename: P M
authors.initials: PM
authors.lastname: Clark
authors.forename: C N
authors.initials: CN
authors.lastname: Hales
mesh_terms: D000469:Alkaline Phosphatase; D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000097:blood* / Q000276:immunology; D011384:Proinsulin / Q000097:blood* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D007328:Insulin; D011384:Proinsulin; D000469:Alkaline Phosphatase