A self peptide naturally presented by both H-2Kb and H-2Kbm1 molecules demonstrates MHC restriction of self tolerance at the molecular level.
An alloreactive CTL clone, 27.B2, produced in the mouse strain combination B6.C-H-2bm1 (bm1) against C57BL/6 (B6) and nominally directed against the Kb molecule has been shown to be peptide dependent. The clone recognizes intact B6, but not intact bm1 or bm3 target cells. However, peptides recognized by 27.B2 CTL can be extracted from bm1 or B6 whole cell lysates as well as from purified Kbm1 or Kb molecules. The target peptides recognized by 27.B2 appear to be identical, whether isolated from Kbm1- or Kb-expressing cells, as shown by identical behavior upon high-resolution reversed-phase HPLC separation. Thus, the same peptide is presented by different MHC molecules including Kb and Kbm1, but is recognized by 27.B2 CTL only in the context of the foreign molecule, Kb, and not in the context of the self molecule, Kbm1.
Falk
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Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany.
Rötzschke
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Rammensee
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D000818:Animals; D002999:Clone Cells / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology*; D007108:Immune Tolerance; D051379:Mice; D008815:Mice, Inbred Strains; D010455:Peptides / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D010455:Peptides