title: Zinc, a novel structural element found in the family of bacterial adenylate kinases.
abstract: The adk gene from Bacillus stearothermophilus was cloned and overexpressed in Escherichia coli under the control of the lac promoter. The primary structure of B. stearothermophilus adenylate kinase exhibited 76% identity with the enzyme from Bacillus subtilis, 60% identity with the enzyme from Lactococcus lactis, and 42% identity with the enzyme from E. coli. The most striking property of the adenylate kinase from B. stearothermophilus is the presence of a structural zinc atom bound to four cysteines in a zinc finger-like fashion. The ability to coordinate zinc is predicted also for a number of other isoforms of bacterial adenylate kinases. Furthermore, the tightly bound metal ion contributes to the high thermodynamic stability of adenylate kinase from B. stearothermophilus.
authors.affiliation: Unité de Régulation de l'Expression Génétique, Institut Pasteur, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Glaser
authors.forename: E
authors.initials: E
authors.lastname: Presecan
authors.forename: M
authors.initials: M
authors.lastname: Delepierre
authors.forename: W K
authors.initials: WK
authors.lastname: Surewicz
authors.forename: H H
authors.initials: HH
authors.lastname: Mantsch
authors.forename: O
authors.initials: O
authors.lastname: Bârzu
authors.forename: A M
authors.initials: AM
authors.lastname: Gilles
mesh_terms: D000263:Adenylate Kinase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D004795:Enzyme Stability; D005798:Genes, Bacterial; D005822:Genetic Vectors; D001411:Geobacillus stearothermophilus / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D011485:Protein Binding; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D015032:Zinc / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D000263:Adenylate Kinase; D015032:Zinc