title: Retrovirus transformation associated secretory phosphoproteins of mouse and mink cells.
abstract: Using antisera to major excreted protein (MEP) of Kirsten sarcoma virus transformed NIH 3T3 (KNIH) cells, we have identified phosphoproteins from the media of feline sarcoma virus (FeSV) transformed mink cells. These secretory phosphoproteins from FeSV-transformed mink cells are of 35 kDa M.W. and they do not have autophosphorylation activity. A comparative analysis of MEP from the media of transformed mouse and mink cells was performed on the basis of proteolytic cleavage products and acid hydrolyzed products of the phosphoproteins. While a marked difference was observed in the peptide map, a common 32P-linked molecule was observed following acid hydrolysis of both species of phosphoproteins.
authors.affiliation: Department of Anatomy and Cell Biology, State University of New York, Brooklyn 11203.
authors.forename: K
authors.initials: K
authors.lastname: Ganguly
authors.forename: M
authors.initials: M
authors.lastname: Essex
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003470:Culture Media; D007708:Kirsten murine sarcoma virus / Q000502:physiology*; D051379:Mice; D008907:Mink; D009858:Oncogenic Viruses / Q000502:physiology*; D010750:Phosphoproteins / Q000032:analysis* / Q000276:immunology; D015682:Retroviridae Proteins, Oncogenic / Q000032:analysis* / Q000276:immunology
chemical_list: D003470:Culture Media; D010750:Phosphoproteins; D015682:Retroviridae Proteins, Oncogenic