title: Determination of vincamine in human plasma by high-performance liquid chromatography with ultraviolet detection.
abstract: A simple and rapid high-performance liquid chromatographic method was developed for the determination of vincamine in human plasma. Plasma samples were buffered at pH 9 and after extraction with tert.-butyl methyl ether back-extracted into 0.017 M orthophosphoric acid. Propranolol was used as the internal standard. An aliquot was injected on to a high-performance liquid chromatographic system using a C18 reversed-phase column and an acetonitrile-phosphate buffer containing triethylamine (30:70) as mobile phase. Detection was performed with an ultraviolet detector at 273 nm. The method had good accuracy and precision and the detection limit (0.3 ng/ml with a signal-to-noise ratio of 3:1) allowed the assessment of vincamine concentrations in plasma in pharmacokinetic studies on healthy human volunteers.
authors.affiliation: B. T. Biotecnica s.r.l., Saronno-Varese, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Dal Bo
authors.forename: G
authors.initials: G
authors.lastname: Ceriani
authors.forename: G
authors.initials: G
authors.lastname: Broccali
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012015:Reference Standards; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014749:Vincamine / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D012996:Solutions; D014749:Vincamine