Evaluation of tumour and tissue distribution of porphyrins for use in photodynamic therapy.
A range of pure, monomeric porphyrins were synthesised and their localising capacities compared to HpD and Hp at 6 h and 24 h post injection in the mouse C6 intracerebral glioma model as well as in normal brain, skin, muscle, kidney, spleen, liver, lung and whole blood. The partition coefficients were examined between PBS and 2-octanol over the pH range 7.4-6.6 and pH profiles were established. A parabolic relationship was observed between log (porphyrin tumour concentration) at pH 7.4, with maximal tumour localisation at log (partition coefficient), pi, of approximately zero. Porphyrins with side chains with nett cationic character also exhibited up upward (parabolic) dependence on pi for most tissues studied, with maximal porphyrin localisation at pi of 0-0.5. In contrast, those porphyrins with nett anionic character exhibited a downward (negative) parabolic trend for all eight tissues studied, with minimal porphyrin localisation at pi of approximately zero. Four porphyrins (4, 11, 12, 13) exhibited similar or better tumour localisation than HpD, and two (11 and 12) offer promise as lead compounds for the design of improved porphyrins for use in PDT.
Woodburn
K W
KW
Biochemistry Department, La Trobe University, Bundoora, Melbourne, Australia.
Stylli
S
S
Hill
J S
JS
Kaye
A H
AH
Reiss
J A
JA
Phillips
D R
DR
D000818:Animals; D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism*; D002460:Cell Line; D005260:Female; D005910:Glioma / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007276:Injections, Intraventricular; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009132:Muscles / Q000378:metabolism; D009374:Neoplasms, Experimental; D011166:Porphyrins / Q000097:blood / Q000493:pharmacokinetics*; D011838:Radiation-Sensitizing Agents / Q000378:metabolism*; D012867:Skin / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution
D011166:Porphyrins; D011838:Radiation-Sensitizing Agents