Production of the cysteine proteinase inhibitor cystatin C by rat Sertoli cells.
The Sertoli cells of the rat testis produce cystatin C, a cysteine proteinase inhibitor. Primary culture of Sertoli cells secreted both unglycosylated and glycosylated forms of rat cystatin C. Despite the low concentration of cystatin C in rete testis fluid, equilibrium dissociation constants (Ki) for the interaction between cystatin C and lysosomal cathepsins indicate that this molecule could be involved in the local regulation of testicular cysteine proteinase activity which may be necessary for spermatogenesis and spermiogenesis.
Esnard
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A
Laboratoire d'Enzymologie et Chimie des Protéines, URA CNRS 1334, Université François Rabelais, Tours, France.
Esnard
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Guillou
F
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Gauthier
F
F
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D055316:Cystatin C; D015891:Cystatins / Q000096:biosynthesis* / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D007985:Leydig Cells / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000378:metabolism*
C525032:Cst3 protein, rat; D055316:Cystatin C; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors