In situ optical histochemistry of human artery using near infrared Fourier transform Raman spectroscopy.
In this paper we demonstrate that near infrared Fourier transform Raman spectroscopy provides unprecedented biochemical information about the extent of atherosclerosis in human aorta. In particular, elastin, collagen, cholesterol, cholesterol esters, lipids, carotenoids, and calcium apatite deposits all can be discerned by using this technique, permitting study of each stage in the disease process. Additionally, these moieties can be detected over 1.5 mm below the irradiated surface of the tissue, possibly allowing extraction of three-dimensional information about the histology of atherosclerotic plaques. We propose that this technique may be utilized for in situ optical histochemical analysis of atherosclerosis in particular and human disease in general.
Baraga
J J
JJ
George R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge 02139.
Feld
M S
MS
Rava
R P
RP
D001011:Aorta / Q000378:metabolism / Q000473:pathology*; D001031:Apatites / Q000032:analysis; D001161:Arteriosclerosis / Q000473:pathology*; D002114:Calcinosis / Q000473:pathology*; D002118:Calcium / Q000032:analysis; D003094:Collagen / Q000032:analysis; D004549:Elastin / Q000032:analysis; D005583:Fourier Analysis; D006801:Humans; D008055:Lipids / Q000032:analysis; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology*; D012016:Reference Values; D013059:Spectrum Analysis, Raman / Q000379:methods
D001031:Apatites; D008055:Lipids; D003094:Collagen; D004549:Elastin; D002118:Calcium