Purification and cDNA cloning of a novel factor produced by a human T-cell hybridoma: sequence homology with animal lectins.
We have purified a novel immunoregulatory factor (BMPG: bone-marrow proteoglycan) produced by a T-cell hybridoma, with a monoclonal antibody column. Using an oligonucleotide probe corresponding to the partial amino acid sequence of BMPG, we cloned, sequenced, and expressed a cDNA for BMPG. BMPG has 222 amino acid residues with a 16 N-terminal signal sequence, so the mature form has 206 amino acid residues. BMPG was found to have unique characteristics: it has three types of sugar chains and it shows a marked homology with animal lectins including the human asialoglycoprotein receptor, chicken hepatic lectin and the homing receptor of lymphocytes.
Yoshimatsu
K
K
Tsukuba Research Laboratory, Eisai Co., Ltd, Ibaragi, Japan.
Ohya
Y
Y
Shikata
Y
Y
Seto
T
T
Hasegawa
Y
Y
Tanaka
I
I
Kawamura
T
T
Kitoh
K
K
Toyoshima
S
S
Osawa
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D047093:Eosinophil Major Basic Protein; D006801:Humans; D006825:Hybridomas; D007694:Killer Cells, Natural / Q000276:immunology; D037102:Lectins / Q000235:genetics*; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D011509:Proteoglycans / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000378:metabolism
D001798:Blood Proteins; D037102:Lectins; D011509:Proteoglycans; C074129:PRG2 protein, human; D004247:DNA; D047093:Eosinophil Major Basic Protein