Brain 4-aminobutyrate aminotransferase. Isolation and sequence of a cDNA encoding the enzyme.
4-Aminobutyrate aminotransferase is a key enzyme of the 4-aminobutyric acid shunt. It is responsible for the conversion of the neurotransmitter 4-aminobutyrate to succinic semialdehyde. By using oligonucleotide probes based on partial amino acid sequence data for the pig brain enzyme, several overlapping cDNA clones of 2.0-2.2 kilobases in length have been isolated. The largest cDNA clone was selected for sequence analysis. The amino acid sequence predicted from the cDNA sequence shows that the precursor of 4-aminobutyrate aminotransferase consists of the mature enzyme of 473 amino acid residues and an amino-terminal segment of 27 amino acids attributed to the signal peptide. The cofactor pyridoxal-5-P is bound to lysine residue 330 of the deduced amino acid sequence of the mature enzyme.
Kwon
O S
OS
Department of Biochemistry, University of Tennessee, Knoxville 37916.
Park
J
J
Churchich
J E
JE
D000612:4-Aminobutyrate Transaminase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D004247:DNA / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013552:Swine
D004247:DNA; D000612:4-Aminobutyrate Transaminase