A structurally novel staphylococcal protein A from the V8 strain.
Protein A from the Staphylococcus aureus strain V8 has a molecular mass about 8000 Da less than that of known proteins A. The corresponding gene was cloned and expressed in Escherichia coli. Sequence analysis of this structurally new protein A revealed that it lacked an IgG-binding domain (58 amino acids), and that it also lacked two octapeptide repetitions located in the membrane/wall attaching region. Contrary to what had been proposed previously, the first translated amino acid is probably not a leucine, but very likely a methionine located 12 residues upstream.
Finck-Barbançon
V
V
Laboratoire de Toxicologie, Institut de Bactériologie, Faculté de Médecine de l'Université Louis Pasteur, Strasbourg, France.
Prevost
G
G
Mazurier
I
I
Piemont
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004926:Escherichia coli / Q000235:genetics; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016364:Reading Frames; D012689:Sequence Homology, Nucleic Acid; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics*
D003062:Codon; D013205:Staphylococcal Protein A