Distinguishing between respiratory syncytial virus subgroups by protein profile analysis.
We subgrouped 75 strains of respiratory syncytial virus by a protein profile method (PPM) which relies on different mobilities of the phosphoprotein in one-dimensional polyacrylamide gel electrophoresis and does not require monoclonal antibodies. When compared with enzyme immunoassay, PPM correctly subgrouped 54 of 56 subgroup A and all 19 subgroup B strains.
Walpita
P
P
Department of Pediatrics, University of California-San Diego, La Jolla 92093.
Mufson
M A
MA
Stanek
R J
RJ
Pfeifer
D
D
Connor
J D
JD
D000956:Antigens, Viral / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D015707:HN Protein; D006801:Humans; D010750:Phosphoproteins / Q000302:isolation & purification; D012136:Respiratory Syncytial Viruses / Q000145:classification* / Q000302:isolation & purification; D010253:Respirovirus Infections / Q000382:microbiology; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000302:isolation & purification*; D014773:Virology / Q000379:methods
D000956:Antigens, Viral; D015707:HN Protein; D010750:Phosphoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G