title: Identification of subgroups of bovine respiratory syncytial virus.
abstract: The occurrence of antigenic variation among nine isolates of bovine respiratory syncytial virus (BRSV) was determined by examining their reaction patterns to human respiratory syncytial virus (HRSV) subgroup A and B monoclonal antibodies (MAbs) by enzyme immunoassay and radioimmunoprecipitation with fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis by using MAbs and polyclonal antisera to HRSV and BRSV. Shared epitopes were demonstrated on four of five structural proteins between BRSV and both subgroups A and B of HRSV. The nine isolates of BRSV showed different patterns of reactivity in enzyme immunoassays with panels of MAbs to HRSV subgroups A and B. Major variations in the molecular weights of the P (phosphoprotein) and F (fusion protein) proteins were demonstrated among the BRSV isolates tested. These results suggest that BRSV belongs to a different antigenic grouping than HRSV and that BRSV is composed of two distinct subgroups.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824.
authors.forename: J C
authors.initials: JC
authors.lastname: Baker
authors.forename: E G
authors.initials: EG
authors.lastname: Wilson
authors.forename: G L
authors.initials: GL
authors.lastname: McKay
authors.forename: R J
authors.initials: RJ
authors.lastname: Stanek
authors.forename: W J
authors.initials: WJ
authors.lastname: Underwood
authors.forename: L F
authors.initials: LF
authors.lastname: Velicer
authors.forename: M A
authors.initials: MA
authors.lastname: Mufson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D002417:Cattle; D015707:HN Protein; D006801:Humans; D007124:Immunoenzyme Techniques; D011233:Precipitin Tests; D012136:Respiratory Syncytial Viruses / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D015707:HN Protein; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C116204:attachment protein G