Construction of physical and genetic maps of Chlamydia trachomatis serovar L2 by pulsed-field gel electrophoresis.
We constructed the physical map of Chlamydia trachomatis serovar L2 by using three restriction endonucleases, NotI (GC[GGCCGC), SgrAI (C(A/G)[CCGG(T/G)G), and Sse8387I (CCTGCA[GG), and we analyzed the fragments by pulsed-field gel electrophoresis. A total of 25 restriction endonuclease sites and 13 genes and/or operons were located on the map. The genome size was determined to be 1,045 kb. Neither highly transcribed chlamydia genes nor developmental cycle-specific genes were clustered on the genome.
Birkelund
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Francis I. Proctor Foundation for Research in Ophthalmology, University of California, San Francisco 94143-0412.
Stephens
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D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012703:Serotyping
D015342:DNA Probes; C064435:endodeoxyribonuclease SgrAI; C074158:endodeoxyribonuclease Sse8387I; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases