Experimental autoimmune dacryoadenitis: purification and characterization of a lacrimal gland antigen.
A soluble lacrimal gland antigen (LG-Ag) capable of inducing experimental autoimmune dacryoadenitis (EAD) in SJL/J mice has been purified from bovine lacrimal gland extracts by a combination of ion exchange and gel-filtration chromatography. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed a single band with a mobility corresponding to a molecular weight of 45,000 daltons. Antibodies raised in guinea pigs against purified LG-Ag were used to confirm the tissue-specificity and to assess the cellular localization of LG-Ag within tissues. Anti-LG-Ag serum reacted with the crude lacrimal gland extracts but not with the extracts of bovine submaxillary gland, parotid gland, or retina. As shown by immunofluorescent staining, LG-Ag appeared to be localized on the murine lacrimal ductal epithelial cells but was lacking in the salivary ducts. LG-Ag induced EAD that was characterized by infiltration of mononuclear cells around the ducts and associated vasculature, accompanied by extensive damage of the acinar cells. The lesions induced were tissue-specific, in that SJL/J mice immunized with LG-Ag had intense lymphocytic infiltrates in lacrimal gland, but had no significant lesions in their salivary and harderian glands. The availability of a purified LG antigen can facilitate the further analysis of pathogenetic mechanisms in EAD.
Liu
S H
SH
Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Zhou
D H
DH
D000818:Animals; D001324:Autoantigens / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003607:Dacryocystitis / Q000276:immunology*; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005455:Fluorescent Antibody Technique; D007765:Lacrimal Apparatus / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008970:Molecular Weight
D001324:Autoantigens