Cytoplasmic microtubule assembly is altered in cytoplasts.
Nucleation of microtubule (MT) organization of the cytoplasmic microtubule complex (CMTC) from the microtubule organizing centres (MTOC) was studied in enucleated cytoplasts of human diploid fibroblast (MRC-5) and mouse peritoneal macrophages in culture. Cytoplasts of both cell types could not organize the complete CMTC. Aberrant MT patterns were seen in MRC-5 cells while mouse macrophages showed occurrence of few short MT. The studies suggest that nucleus may have a role in determining CMTC.
Guha Roy
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Cancer Research Institute, Tata Memorial Centre, Parel, India.
Bhisey
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D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000648:ultrastructure; D003571:Cytochalasin B / Q000494:pharmacology*; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000648:ultrastructure; D051379:Mice; D008866:Microsurgery; D008870:Microtubules / Q000737:chemistry / Q000187:drug effects*; D015739:Nocodazole / Q000494:pharmacology*
D003571:Cytochalasin B; D015739:Nocodazole