title: Evaluation of reversed-phase liquid chromatographic columns for recovery and selectivity of selected carotenoids.
abstract: Sixty commercially available and five experimental liquid chromatography columns were evaluated for the separation and recovery of seven carotenoid compounds. Methanol- and acetonitrile-based solvents (either straight or modified with ethyl acetate or tetrahydrofuran) were compared to determine which solvent systems and which columns provided better selectivity and recovery. Methanol-based solvents typically provided higher recoveries than did acetonitrile-based solvents. Polymeric C18 phases generally provided better selectivity for the difficult separation of lutein and zeaxanthin than did monomeric C18 phases.
authors.affiliation: Organic Analytical Research Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899.
authors.forename: K S
authors.initials: KS
authors.lastname: Epler
authors.forename: L C
authors.initials: LC
authors.lastname: Sander
authors.forename: R G
authors.initials: RG
authors.lastname: Ziegler
authors.forename: S A
authors.initials: SA
authors.lastname: Wise
authors.forename: N E
authors.initials: NE
authors.lastname: Craft
mesh_terms: D002338:Carotenoids / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D013057:Spectrum Analysis
chemical_list: D002338:Carotenoids