title: Glycine, leucine, and phenylalanine flux in low-birth-weight infants during parenteral and enteral feeding.
abstract: Kinetics of three amino acids with different sites and characteristics of metabolic regulation were studied in low-birth-weight infants during enteral and parenteral feeding regimens typical of clinical practice. Primed constant infusions of [15N]glycine, L-[1-13C]leucine, and L-[1-13C]phenylalanine were administered simultaneously by the same route as the feeding, with isotope enrichment measured in urine over 12 h. The effect of feeding regimen was specific to each amino acid (mean +/- SD): glycine flux was lower during parenteral feeding (470 +/- 15 vs 561 +/- 69 mumol.kg-1.h-1, P less than 0.05), leucine flux was unaffected (360 +/- 77 vs 388 +/- 78 mumol.kg-1.h-1), and phenylalanine flux was higher (106 +/- 29 vs 56 +/- 6 mumol.kg-1.h-1, P less than 0.01). Kinetics were influenced by the interaction of several factors, including amino acid intake and routes of feeding and tracer administration. Glycine was most affected by route of feeding and phenylalanine was most affected by intake whereas leucine was little affected. Estimates of whole-body protein turnover calculated from leucine and phenylalanine were different; thus calculations of protein turnover from kinetics of a single amino acid should be interpreted with caution.
authors.affiliation: Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: L J
authors.initials: LJ
authors.lastname: Wykes
authors.forename: R O
authors.initials: RO
authors.lastname: Ball
authors.forename: C E
authors.initials: CE
authors.lastname: Menendez
authors.forename: D M
authors.initials: DM
authors.lastname: Ginther
authors.forename: P B
authors.initials: PB
authors.lastname: Pencharz
mesh_terms: D000596:Amino Acids / Q000008:administration & dosage / Q000378:metabolism*; D004044:Dietary Proteins / Q000008:administration & dosage; D002149:Energy Intake; D004750:Enteral Nutrition; D005998:Glycine / Q000008:administration & dosage / Q000378:metabolism; D006801:Humans; D007230:Infant, Low Birth Weight / Q000378:metabolism*; D007231:Infant, Newborn; D007930:Leucine / Q000008:administration & dosage / Q000378:metabolism; D010288:Parenteral Nutrition; D010649:Phenylalanine / Q000008:administration & dosage / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004044:Dietary Proteins; D010649:Phenylalanine; D007930:Leucine; D005998:Glycine