title: Effect of anti-DNP IgG1- and IgG2a-secreting hybridomas in vivo on the development of an anti-DNP IgE antibody response in mice.
abstract: We reported previously that CBA mice pretreated with dinitrophenyl-Bordetella pertussis (DNP-BP) conjugates exhibited sharply decreased anti-DNP IgE, and increased IgG2a antibodies following immunization with DNP-ovalbumin (DNP-OA) in alum. The objective of the present experiments was to determine whether the decrease in anti-DNP IgE was attributed to a regulatory effect exerted by IgG2a antibodies. Anti-DNP monoclonal antibodies (Mab) of the IgG1 or IgG2a isotype were passively transferred to mice, 24 h before a primary immunization with DNP-OA in alum. Anti-DNP IgE production was drastically suppressed in recipients of IgG1 but not of IgG2a Mab. Similar results were obtained when the Mab were endogenously produced by intraperitoneal implantation of anti-DNP-secreting hybridomas into (BALB/cxCBA)F1 (BCF1) mice. However, neither IgG1 nor IgG2a isotypes suppressed IgE antibody production if the hybridoma implantation took place 10 days after hapten priming. These results are, to our knowledge, the first to show a clear dissociation between the effect of either passively transferred or endogenously secreted IgG1 and IgG2a antibodies in their ability to inhibit a primary anti-hapten IgE antibody response.
authors.affiliation: Department of Microbiology, University of Western Ontario, London, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Hagen
authors.forename: B
authors.initials: B
authors.lastname: Morrison
authors.forename: D
authors.initials: D
authors.lastname: Robbinson
authors.forename: G H
authors.initials: GH
authors.lastname: Strejan
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D004140:Dinitrophenols / Q000276:immunology*; D005260:Female; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D006825:Hybridomas / Q000378:metabolism / Q000637:transplantation; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000378:metabolism*; D007074:Immunoglobulin G / Q000502:physiology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004140:Dinitrophenols; D006241:Haptens; D007074:Immunoglobulin G; C054401:trinitrophenyl keyhole limpet hemocyanin; D007073:Immunoglobulin E; D006433:Hemocyanins