title: Qualitative differences in nuclear proteins correlate with neuronal terminal differentiation.
abstract: 1. Protein composition of neuronal nuclei was studied at two stages of brain maturation, i.e., before (embryonic day 16; E16) and after (postnatal day 10; P10) shortening of the nucleosomal repeat length. Glial nuclei were analyzed in parallel as a control. 2. Total nuclear or HCl- and 5% perchloric acid (PCA)-soluble proteins were analyzed by different electrophoretic techniques. 3. Our results show an increase in the concentration of histone H1 zero with differentiation, although the H1 class undergoes an overall decrease. 4. The chromatin of mature neurons is also enriched in the ubiquinated form of histone H2A (A24), while the high-mobility group (HMG) proteins 1 and 2 seem to decrease slightly relative to core histones. 5. Both quantitative and qualitative differences in the abundance of nonhistone proteins relative to histones accompany neuronal terminal differentiation.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo Alberto Monroy, Università degli Studi, Palermo, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cestelli
authors.forename: D
authors.initials: D
authors.lastname: Castiglia
authors.forename: C
authors.initials: C
authors.lastname: Di Liegro
authors.forename: I
authors.initials: I
authors.lastname: Di Liegro
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002843:Chromatin / Q000737:chemistry; D006609:High Mobility Group Proteins / Q000032:analysis; D006657:Histones / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000737:chemistry / Q000166:cytology*; D009687:Nuclear Proteins / Q000032:analysis*; D009707:Nucleosomes / Q000648:ultrastructure; D051381:Rats
chemical_list: D002843:Chromatin; D006609:High Mobility Group Proteins; D006657:Histones; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D009707:Nucleosomes