Technical note: preservation of tissues and gastrointestinal tract portions by plastic coating or plastination.
Two methods to preserve gastrointestinal tract (GIT) organs and tissues, plastic coating (PC) and plastination (PN), were investigated and compared. Specimens to be preserved were removed from animals within 2 h of death and immediately cleaned with water. Digesta contents were removed by flushing desired portions of GIT with water until the exiting water was clear. In the PC method, cleaned specimens were dehydrated by immersion in an isopropanol solution, dried with forced air after positioning and orientation as in situ, and finally coated on the outer and inner surfaces with a clear plastic material. In the PN procedure, specimens were filled with, and submerged in, a low-formaldehyde fixative, then dehydrated by immersion in a cold acetone solution. Dehydrated specimens were immersed in silicone and placed in a freeze drier for impregnation under low vacuum, followed by overnight gas curing with a silicone crosslinker. Finally, viewing windows were cut out with a scalpel in GIT preserved by both methods. Preserved GIT and tissues had an appearance similar to their appearance in vivo. The PC method was simple and inexpensive. Plastinated specimens were more flexible, durable, and lifelike than those preserved by the PC method. In addition, many body parts, such as muscles, nerves, bones, ligaments, and central nervous system specimens, were preserved by PN. Both methods were found to be useful tools for postmortem studies of tissues and GIT organs.
Pond
K R
KR
Department of Animal Science, College of Veterinary Medicine, North Carolina State University, Raleigh 27695-7621.
Holladay
S D
SD
Luginbuhl
J M
JM
D000433:1-Propanol; D000096:Acetone; D000818:Animals; D002417:Cattle / Q000033:anatomy & histology; D003890:Desiccation; D004064:Digestive System / Q000033:anatomy & histology*; D005612:Freeze Drying; D006736:Horses / Q000033:anatomy & histology; D010969:Plastics; D011309:Preservation, Biological / Q000379:methods / Q000662:veterinary*; D012756:Sheep / Q000033:anatomy & histology; D012828:Silicones; D013552:Swine / Q000033:anatomy & histology
D010969:Plastics; D012828:Silicones; D000096:Acetone; D000433:1-Propanol