Sequence analysis of linked maize 22 kDa alpha-zein genes.
We have determined the nucleotide sequence of a 7343 bp zein genomic clone (gZ22.8H3) from the maize inbred W64A. Computer-aided analysis of the DNA sequence revealed two contiguous 22 kDa alpha-zein genes. The 5' gene (gZ22.8) encodes a complete polypeptide and contains putative regulatory sequences in both the 5' and 3' flanking regions that are typical of zein genes. In contrast, the 3' gene (psi gZ22.8) appears to be a pseudogene, because it contains numerous insertions and deletions that would prevent translation of the mRNA. Alignment of the 5' and 3' flanking sequences of both genes indicated that they resulted from a 3.3 kb DNA duplication event.
Thompson
G A
GA
Department of Plant Sciences, University of Arizona, Tucson 85721.
Siemieniak
D R
DR
Sieu
L C
LC
Slightom
J L
JL
Larkins
B A
BA
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D003313:Zea mays / Q000235:genetics*; D015028:Zein / Q000235:genetics*
D004247:DNA; D015028:Zein