Effect of a 5 alpha-reductase inhibitor on the metabolism of 19-norandrogens by porcine Leydig cells.
The metabolism of 19-norandrostenedione and [3H] 19-nortestosterone was examined in porcine Leydig cell preparations in the absence, or presence, of a 4-azasteroid inhibitor of 5 alpha-reductase. Evidence for a major production of 5 alpha-estrane-3 beta, 17 beta-diol and 3 beta-hydroxy-5 alpha-estran-17-one, as sulfo-conjugated steroids, was obtained by HPLC and gas-chromatography/mass spectrometry. The 4-aza-steroid clearly reduced the formation of both of the above 5 alpha-reduced products from the 19-norandrogens. From the HPLC profiles of the radioactive metabolites, it was also concluded that estrogen secretion was increased significantly by exposure of the cells to the 5 alpha-reductase inhibitor.
Raeside
J I
JI
Department of Biomedical Sciences, University of Guelph, Ontario, Canada.
Renaud
R L
RL
Marshall
D E
DE
D058891:5-alpha Reductase Inhibitors; D000735:Androstenedione / Q000031:analogs & derivatives / Q000378:metabolism; D000736:Androstenes / Q000378:metabolism*; D000818:Animals; D002469:Cell Separation; D002851:Chromatography, High Pressure Liquid; D004967:Estrogens / Q000096:biosynthesis*; D008401:Gas Chromatography-Mass Spectrometry; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D008299:Maleic Anhydrides / Q000494:pharmacology*; D009277:Nandrolone / Q000378:metabolism; D013552:Swine
D058891:5-alpha Reductase Inhibitors; D000736:Androstenes; D004967:Estrogens; D008299:Maleic Anhydrides; D000735:Androstenedione; D009277:Nandrolone; C007474:2,3-dimethylmaleic anhydride; C002828:19-norandrostenedione