Phospholipid base exchange enzyme activity in sarcolemmal membranes from the heart of cardiomyopathic hamsters.
The activity of phospholipid base exchange enzymes has been evaluated in cardiac sarcolemmal membranes from Syrian Golden hamsters and from a hamster strain (UM-X7.1) characterized by a genetic form of hypertrophic cardiomyopathy. No choline base exchange activity and only a little serine base exchange activity were detected, whereas the ethanolamine base exchange enzyme was found highly active in membranes from both strains. For this reason, the present study is focussed on the ethanolamine base exchange enzyme. The apparent Km for ethanolamine of ethanolamine base exchange enzyme from Syrian Golden membranes and from UM-X7.1 strain membranes are 18 and 32 microM, respectively. The specific activity of the sarcolemmal ethanolamine base exchange enzyme is lower in the UM-X7.1 strain than in Syrian Golden hamsters. The calcium-dependence of the enzyme appears different when the membranes from the two strains are compared. Indeed, after removal of the membrane-bound divalent cations, comparable activities are found in both membrane preparations, whereas, upon addition of Ca2+ to the incubation mixtures, the activity of the enzyme is enhanced in the membranes from Syrian Golden strain more than in those from UM-X7.1 strain. The cholesterol content of sarcolemmal membranes is higher in the cardiomyopathic strain than in the Syrian Golden hamsters. A possible relation between changes of the membrane lipid composition and of the ethanolamine base exchange activity is discussed.
Vecchini
A
A
Department of Biochemistry, University of Perugia, Italy.
Binaglia
L
L
Di Nardo
P
P
Minieri
M
M
Tallarida
G
G
D000818:Animals; D002312:Cardiomyopathy, Hypertrophic / Q000201:enzymology*; D006224:Cricetinae; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism; D008647:Mesocricetus; D009206:Myocardium / Q000201:enzymology*; D010743:Phospholipids / Q000378:metabolism*; D012508:Sarcolemma / Q000201:enzymology*
D004983:Ethanolamines; D010743:Phospholipids; D019856:Ethanolamine