Comparison of liver glycosylasparaginases from six vertebrates.
Structural and physical properties of glycosylasparaginase (EC 3.5.1.26) from the livers of human, pig, cow, rat, mouse and chicken were compared. The enzyme in all species had a common basic structure of two N-glycosylated subunits of about 24 (alpha) and 20 (beta) kDa joined by non-covalent forces. Subunit-specific antisera against the rat glycosylasparaginase bound specifically and sensitively to the corresponding subunits from all species. Identity of 80% of the amino acids was found between the N-terminal sequences of corresponding pig and rat glycosylasparaginase alpha- and beta-subunits and the deduced sequence from a human glycosylasparaginase cDNA [Fisher, Tollersrud & Aronson (1990) FEBS Lett. 269, 440-444]. The beta-subunit from all three species has an N-terminal threonine reported to be involved in the reaction mechanism for the human enzyme [Kaartinen, Williams, Tomich, Yates, Hood & Mononen (1991) J. Biol. Chem. 266, 5860-5869]. The native enzyme appeared as a heterodimer among the mammals, whereas the chicken enzyme had a greater molecular mass and is probably either a tetramer or a heterodimer bound to an unrelated peptide(s). All glycosylasparaginases were thermostable, requiring temperatures between 65 degrees C and 80 degrees C to be irreversibly inactivated. In addition, they were unusually stable at high pH and remained active in the presence of SDS except at low pH. The pH maximum was between 5.5 and 6 except for the rat and mouse enzymes which had a broad maximum between pH 7 and 8. A number of other properties were observed which also distinguish the enzyme from individual and closely related species.
Tollersrud
O K
OK
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Aronson
N N
NN
D000595:Amino Acid Sequence; D000818:Animals; D001227:Aspartylglucosylaminase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006031:Glycosylation; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007526:Isoelectric Point; D008099:Liver / Q000201:enzymology; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013552:Swine
D046911:Macromolecular Substances; D001227:Aspartylglucosylaminase