DNA elements target of transcriptional factors are not restricted to long terminal repeat of human immunodeficiency virus.
In this report we show that signals for transcriptional factors are not restricted to the HIV-1 LTR, but are present throughout the HIV-1 genome. Furthermore, we identified a sequence, AGAACAGATG, highly homologous to the X-box of class II MHC genes and located within the tat-IVS/env region of HIV-1. Double stranded oligonucleotides mimicking the HIV-1 region containing AGAACAGATG were synthesized and band shift experiments were performed demonstrating that this HIV-1 genomic region binds nuclear proteins. We further demonstrate that the binding of nuclear factors to this tat-IVS/env HIV-1 sequence is competed for, in the band-shift assay, by the highly homologous X-box of the promoter of the human HLA-DR alpha gene. The presence in the HIV-1 genome of DNA sequences homologous or identical to regulatory sequences of cellular genes represents a potential mechanism of predation of DNA elements recognized by DNA binding proteins.
Feriotto
G
G
Istituto di Chimica Biologica, Università di Ferrara, Italy.
Volinia
S
S
Giacomini
P
P
Gambari
R
R
D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D005802:Genes, MHC Class II; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000502:physiology*
D006684:HLA-DR Antigens; D014157:Transcription Factors; D004247:DNA