Determination of potato glycoalkaloids and their aglycone in blood serum by high-performance liquid chromatography. Application to pharmacokinetic studies in humans.
The development of a high-performance liquid chromatography (HPLC) method for the separation and quantification of potato glycoalkaloids and their aglycone solanidine in blood serum is reported. High selectivity was obtained by using solid-phase extraction followed by off-line dual-column HPLC. Injections were made via a sample enrichment column to achieve maximum sensitivity in the assay. The potato alkaloids in the HPLC effluents were detected by ultraviolet absorption at 200 nm. The detection limits were estimated to be 0.3 ng/ml of serum for each of the alkaloids. The method was used to study the pharmacokinetics of potato glycoalkaloids in humans. alpha-Solanine and alpha-chaconine were detected in all blood serum samples collected from seven volunteers 1-25 h after a meal of potatoes. Solanidine was detected in some samples, but there were no traces of the mono- or diglycosides. The average apparent biological half-lives for alpha-solanine and alpha-chaconine were 11 and 19 h, respectively.
Hellenäs
K E
KE
Department of Plant Husbandry, Swedish University of Agricultural Sciences, Uppsala.
Nyman
A
A
Slanina
P
P
Lööf
L
L
Gabrielsson
J
J
D000328:Adult; D000470:Alkaloids / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004144:Diosgenin; D006207:Half-Life; D006801:Humans; D008297:Male; D012991:Solanaceous Alkaloids / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics; D012992:Solanine / Q000031:analogs & derivatives / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics; D011198:Solanum tuberosum / Q000009:adverse effects / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000470:Alkaloids; D012991:Solanaceous Alkaloids; D012992:Solanine; C011860:alpha-chaconine; D004144:Diosgenin; C013255:solanidine