title: Brain 4-aminobutyrate aminotransferase. Isolation and sequence of a cDNA encoding the enzyme.
abstract: 4-Aminobutyrate aminotransferase is a key enzyme of the 4-aminobutyric acid shunt. It is responsible for the conversion of the neurotransmitter 4-aminobutyrate to succinic semialdehyde. By using oligonucleotide probes based on partial amino acid sequence data for the pig brain enzyme, several overlapping cDNA clones of 2.0-2.2 kilobases in length have been isolated. The largest cDNA clone was selected for sequence analysis. The amino acid sequence predicted from the cDNA sequence shows that the precursor of 4-aminobutyrate aminotransferase consists of the mature enzyme of 473 amino acid residues and an amino-terminal segment of 27 amino acids attributed to the signal peptide. The cofactor pyridoxal-5-P is bound to lysine residue 330 of the deduced amino acid sequence of the mature enzyme.
authors.affiliation: Department of Biochemistry, University of Tennessee, Knoxville 37916.
authors.forename: O S
authors.initials: OS
authors.lastname: Kwon
authors.forename: J
authors.initials: J
authors.lastname: Park
authors.forename: J E
authors.initials: JE
authors.lastname: Churchich
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D004247:DNA / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013552:Swine
chemical_list: D004247:DNA; D000612:4-Aminobutyrate Transaminase