title: Genetic analysis of HIV-1 isolates from Zambia and an expanded phylogenetic tree for HIV-1.
abstract: Geographic variation in the HIV-1 virus is extensive but incompletely documented. We herein report the first genetic characterization of HIV-1 isolates from Zambia. The genomic region encoding the GAG polyprotein has been compared among 22 Zambian isolates and 14 North American isolates using a combination of polymerase chain reaction (PCR) and DNA sequencing methods. The Zambian isolates were similar to one another but distinct from other HIV-1 isolates. They exhibited a characteristic PCR "fingerprint" wherein certain primer combinations were unable to amplify because of mispairing. The sequence of the complete gag gene of three isolates from Zambia has been determined, and phylogenetic tree analysis placed them in a branch distinct from other African isolates and North American isolates. The PCR procedure used here may be widely applicable for genetic characterization of HIV-1.
authors.affiliation: Henry M. Jackson Foundation for the Advancement of Military Medicine, Research Laboratory, Rockville, MD 20850.
authors.forename: F E
authors.initials: FE
authors.lastname: McCutchan
authors.forename: B L
authors.initials: BL
authors.lastname: Ungar
authors.forename: P
authors.initials: P
authors.lastname: Hegerich
authors.forename: C R
authors.initials: CR
authors.lastname: Roberts
authors.forename: A K
authors.initials: AK
authors.lastname: Fowler
authors.forename: S K
authors.initials: SK
authors.lastname: Hira
authors.forename: P L
authors.initials: PL
authors.lastname: Perine
authors.forename: D S
authors.initials: DS
authors.lastname: Burke
mesh_terms: D001483:Base Sequence; D015342:DNA Probes; D015750:Genes, gag; D014644:Genetic Variation; D015497:HIV-1 / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D009656:North America; D015345:Oligonucleotide Probes; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015024:Zambia
chemical_list: D015342:DNA Probes; D015345:Oligonucleotide Probes