title: Solution structures of nisin A and its two major degradation products determined by n.m.r.
abstract: The conformations of nisin and two major degradation products, nisin-(1-32)-peptide (nisin1-32) and des-delta Ala5-nisin1-32 (where delta Ala is alpha beta-didehydroalanine), in aqueous solution have been determined from n.m.r. data. Sequential assignments of the peptides using correlation spectroscopy ('COSY'), homonuclear Hartmann-Hahn spectroscopy ('HOHAHA'), nuclear Overhauser enhancement spectroscopy (NOESY), relayed NOESY and rotating-frame nuclear Overhauser spectroscopy (ROESY) experiments are presented, including stereospecific assignments of beta-methylene protons of the lanthionine residues. ROESY experiments are also used to detect flexible regions in the polypeptide chain. A dynamic-stimulated-annealing approach is used for structural determination. It can be concluded that all these peptides are flexible in aqueous solution, with no experimental evidence of preferred overall conformations; the only defined conformational features are imposed by the presence of the lanthionine residues. Low-temperature studies also reveal that des-delta Ala5-nisin1-32 adopts conformations similar to those when the ring is intact, suggesting that the loss of activity of this degradation product is due to the absence of the delta Ala5 residue rather than to the conformational consequences of ring-opening.
authors.affiliation: Biological NMR Centre, University of Leicester, U.K.
authors.forename: L Y
authors.initials: LY
authors.lastname: Lian
authors.forename: W C
authors.initials: WC
authors.lastname: Chan
authors.forename: S D
authors.initials: SD
authors.lastname: Morley
authors.forename: G C
authors.initials: GC
authors.lastname: Roberts
authors.forename: B W
authors.initials: BW
authors.lastname: Bycroft
authors.forename: D
authors.initials: D
authors.lastname: Jackson
mesh_terms: D000595:Amino Acid Sequence; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009561:Nisin / Q000737:chemistry* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D012996:Solutions
chemical_list: D010446:Peptide Fragments; D012996:Solutions; D009561:Nisin