The effects of thymus supernatant on the thymus homing ability of aged murine bone marrow cells.
This study examined the effect of thymus supernatant on the ability of bone marrow cells from senescent mice to home to the thymus. An in vitro and an in vivo assay were employed. The in vitro assay used a blind well migration of aged bone marrow cells to thymus supernatant across a membrane with 5-microns pores. The in vitro assay measured the ability of aged bone marrow to repopulate the thymus of an irradiated host. Our results support previous reports that the bone marrow from old mice has a greatly reduced ability to migrate to thymus supernatant and to repopulate the thymus of an irradiated host. Further, we found that a brief treatment of the old bone marrow with thymus supernatant significantly improved its thymus homing ability both in vitro and in vivo.
McCormick
K R
KR
Department of Anatomy, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Haar
J L
JL
D000375:Aging / Q000473:pathology / Q000502:physiology*; D000818:Animals; D001854:Bone Marrow Cells; D002465:Cell Movement; D002633:Chemotaxis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008808:Mice, Inbred CBA; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology / Q000502:physiology*