B-cell factor 1 is required for optimal expression of the DRA promoter in B cells.
The X box in the DRA promoter of the human histocompatibility complex is required for expression of the DRA gene in B cells. We show that a B-cell factor binds to a sequence that is clearly distinguishable from binding sites for the previously described X box binding nuclear proteins RF-X, NF-X, NF-Xc, NF-S, hXBP, and AP-1. Mutations in the DRA X box that disrupt the binding of this factor result in a lower level of gene expression, as does the presence of Id (a trans-dominant regulatory protein that negatively regulates helix-loop-helix proteins). Furthermore, this factor is recognized by antibodies directed against the helix-loop-helix protein A1, a mouse homolog of the immunoglobulin enhancer binding proteins E12/E47, and it binds to sequences in other genes that were previously shown to bind these proteins. By these criteria, this factor is BCF-1.
Voliva
C F
CF
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
Aronheim
A
A
Walker
M D
MD
Peterlin
B M
BM
D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology; D002872:Chromosome Deletion; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding
D004268:DNA-Binding Proteins; D006684:HLA-DR Antigens; D009687:Nuclear Proteins