title: Biosynthetic radiolabeling of bacterial lipopolysaccharide to high specific activity.
abstract: We describe a method for producing radiolabeled lipopolysaccharide (LPS) by incorporating [3H]acetate into an aceEF, gltA strain of Escherichia coli K12. The LPS has substantially greater specific radioactivity (2 microCi per microgram LPS, or approximately 8 Ci/mmol) than has been reported previously for biosynthetically radiolabeled LPS. The 3H is incorporated into the fatty acyl chains of the lipid A moiety. LPS prepared by this method has several attractive features for biological studies, including native structure and bioactivity, long radioactive half-life, and high specific activity.
authors.affiliation: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-8859.
authors.forename: R S
authors.initials: RS
authors.lastname: Munford
authors.forename: L C
authors.initials: LC
authors.lastname: DeVeaux
authors.forename: J E
authors.initials: JE
authors.lastname: Cronan
authors.forename: P D
authors.initials: PD
authors.lastname: Rick
mesh_terms: D002478:Cells, Cultured; D004926:Escherichia coli; D007553:Isotope Labeling / Q000379:methods*; D008070:Lipopolysaccharides; D014316:Tritium
chemical_list: D008070:Lipopolysaccharides; D014316:Tritium