Radioimmunodetection of human colon cancer using 99mTc-MDP-MoAb-A7 in mice.
Radioimmunoscintigraphy (RIS) using 99mTc-labeled A7, a monoclonal antibody (MoAb) against a human colorectal cancer, was performed in nude mice bearing a human colon cancer, HCT-15. MoAb-A7 was labeled with 99mTc in the presence of methylene diphosphate (MDP). At 24 h post-injection of 99mTc-MDP-A7, colon cancer was clearly visualized, and the tumor-to-tissue ratio of 99mTc-MDP-A7 was higher than the ratio of 111In-DTPA-A7 which was described previously. These results suggest the possibility of the clinical application of 99mTc-MDP-A7 for RIS of a human colon cancer.
Shimura
N
N
Kojima
S
S
Kubodera
A
A
Kubota
K
K
Takahashi
T
T
Oyamada
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging*; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016719:Radioimmunodetection; D013669:Technetium Tc 99m Medronate; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D013669:Technetium Tc 99m Medronate