title: In vivo and in vitro toxicity of phospholipase C from Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa produces phospholipase C (PLC), a heat-labile hemolysin. Histopathological analysis of PLC-treated mice revealed that the primary target organs involved in PLC-induced toxicity were the liver and kidney. Mice treated i.v. with PLC demonstrated significant tubular epithelial necrosis of the kidney with hematuria, while when given i.p. they exhibited hepatonecrosis with cellular infiltration. Splenomegaly was also a consistent finding. Results from in vitro studies indicate that PLC is toxic for mouse peritoneal cells and human leukocytes.
authors.affiliation: Department of Immunology/Microbiology, Wayne State University School of Medicine, Detroit, MI 48201.
authors.forename: D J
authors.initials: DJ
authors.lastname: Meyers
authors.forename: K C
authors.initials: KC
authors.lastname: Palmer
authors.forename: L A
authors.initials: LA
authors.lastname: Bale
authors.forename: K
authors.initials: K
authors.lastname: Kernacki
authors.forename: M
authors.initials: M
authors.lastname: Preston
authors.forename: T
authors.initials: T
authors.lastname: Brown
authors.forename: R S
authors.initials: RS
authors.lastname: Berk
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism / Q000633:toxicity*; D006321:Heart / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D009206:Myocardium / Q000473:pathology; D009504:Neutrophils / Q000187:drug effects / Q000648:ultrastructure; D010529:Peritoneal Cavity / Q000166:cytology; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D010738:Type C Phospholipases / Q000378:metabolism / Q000633:toxicity*
chemical_list: D001427:Bacterial Toxins; D010738:Type C Phospholipases