Primary structure and functional expression of the Na/Ca,K-exchanger from bovine rod photoreceptors.
Complementary DNA encoding the Na/Ca,K-exchanger was isolated from bovine retina cDNA libraries. The complete full-length cDNA is approximately 4 kb long and contains an open reading frame of 3597 bp. The deduced amino acid sequence corresponds to a protein of 1199 amino acids with a calculated molecular weight of approximately 130 kDa. Hydrophobicity analysis revealed the presence of two alternating sets of hydrophobic and hydrophilic domains. There also exists a hydrophobic region at the N-terminus which may be part of a cleavable signal peptide. The protein shares limited sequence homology with the Na/Ca-exchanger from cardiac sarcolemma. Northern blot analysis indicates that the approximately 6 kb transcript is highly specific for retinal tissue. Insect cells infected with recombinant baculovirus bearing the full-length cDNA express a functional Na/Ca,K-exchanger with an apparent relative molecular weight of approximately 210 kDa, as determined by Western blotting.
Reiländer
H
H
Max-Planck-Institut für Biophysik, Abteilung für Molekulare Membranbiologie, Frankfurt am Main, FRG.
Achilles
A
A
Friedel
U
U
Maul
G
G
Lottspeich
F
F
Cook
N J
NJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D016366:Open Reading Frames; D010786:Photoreceptor Cells / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D019831:Sodium-Calcium Exchanger
D002352:Carrier Proteins; D012333:RNA, Messenger; D019831:Sodium-Calcium Exchanger; C074530:potassium-dependent sodium-calcium exchanger; D004247:DNA