Mutagenic and cytotoxic action of heated pork meat extracts in human diploid fibroblasts.
Extracts from lean pork heated at 200 degrees C have a strong mutagenic activity in the Ames Salmonella assay (strain TA98 +S9). The formation of mutagenicity is highly temperature dependent, thus an extract heated at 100 degrees C is not mutagenic in this system. This paper shows that the 200 degrees C extract also causes mutations at the hprt locus in normal human fibroblasts, as demonstrated by a dose-dependent increase of 6-thioguanine resistant mutants. The mutation frequencies were increased 6 and 13 times, respectively, for extract concentrations corresponding to 100 and 200 mg meat/ml medium. Heterocyclic amines, previously shown to be present in the 200 degrees C extract are conceivably responsible for at least part of the observed mutagenicity. The extracts prepared at 100 degrees C had no significant effect on the mutant frequency in human fibroblasts. A pronounced, dose-dependent, decrease in cell survival was observed with both the 100 and the 200 degrees C extracts. The nature of the cytotoxic components is not clear and might be different in the two extracts.
Kolman
A
A
Department of Radiobiology, Stockholm University, Sweden.
Kleman
M
M
Bohusová
T
T
Gustafsson
J A
JA
Overvik
E
E
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004171:Diploidy; D005347:Fibroblasts; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D008460:Meat / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D013552:Swine
D009153:Mutagens