Intracistronic complementation reveals a new function of SV40 T antigen that co-operates with Rb and p53 binding to stimulate DNA synthesis in quiescent cells.
The ability of the oncogene products of DNA tumor viruses to induce DNA synthesis in quiescent cells is thought to depend on their capacity to bind to cellular proteins such as the retinoblastoma-suppressor protein Rb and the tumor suppressor p53, thereby abolishing the growth-arresting properties of these proteins. We have tested this hypothesis using SV40 T antigens carrying lesions that affect Rb binding, p53 binding or other functions involved in cell transformation. The results demonstrate that Rb binding is not essential for growth stimulation by T antigen. However, detailed analysis, including intracistronic complementation, suggests that at least three functions, Rb binding, a novel second activity localized to the DNA-binding domain and a function residing in the carboxy terminus, probably p53 binding, cooperate to generate the full growth induction potential of T antigen.
Dobbelstein
M
M
Institute for Biochemistry, Munich, Germany.
Arthur
A K
AK
Dehde
S
S
van Zee
K
K
Dickmanns
A
A
Fanning
E
E
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000502:physiology*; D002455:Cell Division / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000276:immunology*; D002874:Chromosome Mapping; D004247:DNA / Q000096:biosynthesis*; D015972:Gene Expression Regulation, Neoplastic; D005816:Genetic Complementation Test; D000882:Haplorhini; D066298:In Vitro Techniques; D010957:Plasmids; D016160:Retinoblastoma Protein / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D000952:Antigens, Polyomavirus Transforming; D016160:Retinoblastoma Protein; D016159:Tumor Suppressor Protein p53; D004247:DNA