Secretion of a chemotactic substance(s) by AGE-stimulated human monocytes.
Receptors for products of non-enzymatic glycosylation have been identified previously on activated human monocytes. In this study we have found that medium conditioned by activated human monocytes following stimulation with AGE-BSA elicited an almost 3-fold greater chemotactic response from other activated monocytes than conditioned medium obtained following stimulation with control BSA (44 +/- 13 and 16 +/- 4.6, respectively; n = 9, P less than 0.05). The response elicited from AGE-BSA alone was not statistically significant. It appears that stimulation of the cells via the AGE-receptor results in the secretion of increased levels of a chemotactic substance(s) for monocytes/macrophages. This mechanism may help to explain the pathogenesis of atherosclerosis in diabetes, as monocyte accumulation within the vessel wall is an important step in fatty streak development.
Gilcrease
M Z
MZ
Department of Pathology, Vanderbilt University, Nashville, Tennessee 37232.
Hoover
R L
RL
D002630:Chemotactic Factors / Q000097:blood* / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D004731:Endotoxins / Q000494:pharmacology; D017127:Glycation End Products, Advanced; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D012709:Serum Albumin / Q000494:pharmacology*; D012710:Serum Albumin, Bovine; D000093423:Glycated Serum Albumin
D002630:Chemotactic Factors; D004731:Endotoxins; D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D000093423:Glycated Serum Albumin