Reactivity to TGF-beta is one step towards transformation in a murine fibroblast cell line.
High doses of TGF-beta induce reactivity to TGF-beta in a distinct number of C3H10T1/2 fibroblasts suspended in soft agar. Reactive cells can be isolated and stably maintain this property. In the absence of exogenously added TGF-beta, these cells do not form colonies, but show distinct changes in their morphology and cytoskeleton. In contrast to normal C3H10T1/2 cells, they are able to form colonies when low doses of TGF-beta are added. TGF-beta-reactive cells are more easily transformed by UV light than normal C3H10T1/2 cells, and so maintain their transformed phenotype in the absence of added TGF-beta. Reactivity for TGF-beta, therefore, represents one step towards transformation.
Götschl
M
M
Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, Germany.
Höfler
P
P
Bauer
G
G
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced* / Q000473:pathology; D003114:Colony-Forming Units Assay; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D051379:Mice; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016212:Transforming Growth Factor beta