Isolation of a hemoglobin-derived opioid peptide from cerebrospinal fluid of patients with cerebrovascular bleedings.
The hemorphins are peptides with opioid activity, which are enzymatically released from hemoglobin. A decapeptide identical to the sequence 32-41 of the beta-, delta-, gamma- or epsilon-chains of hemoglobin has been isolated from human ventricular cerebrospinal fluid (CSF). The peptide, designated LVV-hemorphin-7, was recovered in relatively high amounts (115-300 pmol per ml) from samples of patients with cerebrovascular bleedings, but was not detectable in control CSF. Its identity with the hemoglobin fragment was confirmed by mass spectrometry and gas-phase sequencing.
Glämsta
E L
EL
Department of Pharmacology, University of Uppsala, Sweden.
Meyerson
B
B
Silberring
J
J
Terenius
L
L
Nyberg
F
F
D000595:Amino Acid Sequence; D002543:Cerebral Hemorrhage / Q000134:cerebrospinal fluid*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006408:Hematoma, Subdural / Q000134:cerebrospinal fluid*; D006454:Hemoglobins / Q000134:cerebrospinal fluid* / Q000302:isolation & purification; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000134:cerebrospinal fluid* / Q000302:isolation & purification; D012016:Reference Values; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013345:Subarachnoid Hemorrhage / Q000134:cerebrospinal fluid*
D006454:Hemoglobins; D010446:Peptide Fragments; C070563:hemorphin 6; C074309:LVV-hemorphin-7