Heterologous transformation of Mucor circinelloides with the Phycomyces blakesleeanus leu1 gene.
The leu1 gene of Phycomyces blakesleeanus was isolated within a HindIII-HindIII genomic DNA fragment by heterologous hybridization screening of a cosmid library, making use of the Mucor circinelloides leuA gene as a probe. The complete nucleotide sequence of this fragment reveals a single 2070 bp ORF with no introns, which presents at least 68% homology with that of the leuA gene. The P. blakesleeanus leu1 gene has also been expressed in the M. circinelloides mutant R7B (leu-), which was used to isolate the leuA gene by complementation. The homology with other known sequences shows that the leu1 gene encodes the P. blakesleeanus alpha-IPM (isopropylmalate) isomerase.
Iturriaga
E A
EA
Departamento de Microbiología y Genética, Facultad de Biología, Universidad de Salamanca, Spain.
Díaz-Mínguez
J M
JM
Benito
E P
EP
Alvarez
M I
MI
Eslava
A P
AP
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005821:Genetic Techniques; D015698:Genomic Library; D006836:Hydro-Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D009089:Mucor / Q000235:genetics*; D010800:Phycomyces / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D006836:Hydro-Lyases; C021455:3-isopropylmalate dehydratase