Inhibition of lipoxygenase activity in lentil protoplasts by monoclonal antibodies introduced into the cells via electroporation.
The isolation of lentil protoplasts and the transfer of anti-lipoxygenase monoclonal antibodies into plant protoplasts by electroporation is reported. The dependence of the efficiency of monoclonal antibody incorporation on the field strength is shown as well. The transferred immunoglobulins retained their functional and structural integrity and were able to inhibit the intracellular target enzyme, with a linear relationship between inhibition of lipoxygenase activity and amount of incorporated monoclonal antibody. Moreover, the inhibition of lipoxygenase activity was well correlated with the increase of protoplast viability.
Maccarrone
M
M
Bijvoet Center for Biomolecular Research, Department of Bio-organic Chemistry, Utrecht University, The Netherlands.
Veldink
G A
GA
Vliegenthart
J F
JF
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D004560:Electricity; D004797:Enzyme-Linked Immunosorbent Assay; D007887:Fabaceae / Q000201:enzymology*; D008084:Lipoxygenase / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors / Q000378:metabolism*; D010946:Plants, Medicinal; D011523:Protoplasts / Q000201:enzymology*; D013025:Glycine max / Q000201:enzymology
D000911:Antibodies, Monoclonal; D016859:Lipoxygenase Inhibitors; D008084:Lipoxygenase