title: Influence of serotonin on progesterone and estradiol secretion of cultured human granulosa cells.
abstract: OBJECTIVE
  To explore the direct action of serotonin on progesterone (P) and estradiol (E2) secretion of human granulosa cells cultured in serum-free medium.
  
  
  METHODS
  Progesterone and E2 production was measured in the presence and absence of serotonin, propranolol, or cycloheximide using radioimmunoassays; statistical analysis of the data was performed by ANOVA.
  
  
  METHODS
  In vitro fertilization and embryo transfer (IVF-ET) for infertility treatment at the University Women's Hospital, University of Tübingen, Germany.
  
  
  METHODS
  Fourteen women, 30 +/- 3 years old, undergoing IVF-ET.
  
  
  RESULTS
  Serotonin stimulated a dose-related increase in P secretion with a maximal stimulatory effect at 10(-4) M. This response was blocked specifically by the beta-receptor antagonist propranolol (10(-4) M). Estradiol secretion in response to serotonin was dose-independent stimulation, which was highest at 10(-6) M and was inhibited by 10(-4) M propranolol. The protein synthesis inhibitor cycloheximide markedly reduced the stimulatory effect of serotonin on P secretion. Estradiol production in the presence of cycloheximide was significantly reduced; serotonin had no stimulatory effect under these conditions.
  
  
  CONCLUSIONS
  Serotonin may have a physiological role in the corpus hemorrhagicum, when luteinization is initiated.
authors.affiliation: University Women's Hospital of Tübingen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Bódis
authors.forename: A
authors.initials: A
authors.lastname: Török
authors.forename: H R
authors.initials: HR
authors.lastname: Tinneberg
authors.forename: V
authors.initials: V
authors.lastname: Hanf
authors.forename: M
authors.initials: M
authors.lastname: Hamori
authors.forename: P
authors.initials: P
authors.lastname: Cledon
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D009994:Osmolar Concentration; D011374:Progesterone / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology*
chemical_list: D012701:Serotonin; D011374:Progesterone; D004958:Estradiol; D003513:Cycloheximide; D011433:Propranolol