title: Concanavalin A-nonbinding Aspergillus fumigatus antigen: a major immunogen in allergic bronchopulmonary aspergillosis.
abstract: Humoral immune responses in allergic bronchopulmonary aspergillosis (ABPA) have been well studied. However, reports of cell-mediated immune responses in ABPA are conflicting and not well documented, perhaps because well-characterized antigens are not available. In the present study, we assessed the role of peripheral blood mononuclear cells (PBMCs) from patients with ABPA in their ability to respond to both crude and semipurified Aspergillus fumigatus antigens by in vitro proliferation and immunoglobulin E synthesis. Eight patients with ABPA, eight patients with immediate wheal and flare skin reactivity to A. fumigatus, and ten healthy control subjects with nonreactive skin tests were included in this study. Four A. fumigatus antigens were tested in vitro. Antigens included culture filtrate, mycelial extract, concanavalin A-nonbinding, and concanavalin A-binding antigens. There was a wide range of response to each antigen by each group of subjects. However, PBMCs from patients with ABPA showed greater response to the antigens than did those from the healthy control subjects when evaluated by lymphoproliferation (tritiated thymidine uptake) and immunoglobulin E synthesis (isotype-specific enzyme-linked immunosorbent assay). The concanavalin A-nonbinding antigen fraction had the ability to specifically stimulate proliferation of PBMCs from seven of eight patients with ABPA and from four of eight skin-reactive subjects; none of the healthy control subjects responded. Significantly high levels of immunoglobulin E were detected in unstimulated PBMC cultures from five patients with ABPA when compared with those from healthy control subjects. These results indicate that concanavalin A-nonbinding A. fumigatus antigens may be significant in the cellular immune response of ABPA; such results are similar to those from previous humoral studies of the disease.
authors.affiliation: Research Service, Zablocki Veterans Administration Medical Center, Medical College of Wisconsin, Milwaukee 53295.
authors.forename: P S
authors.initials: PS
authors.lastname: Murali
authors.forename: V P
authors.initials: VP
authors.lastname: Kurup
authors.forename: P A
authors.initials: PA
authors.lastname: Greenberger
authors.forename: J N
authors.initials: JN
authors.lastname: Fink
mesh_terms: D000946:Antigens, Fungal / Q000737:chemistry / Q000378:metabolism*; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000276:immunology*; D001232:Aspergillus fumigatus / Q000276:immunology*; D003208:Concanavalin A / Q000378:metabolism*; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000946:Antigens, Fungal; D003208:Concanavalin A; D007073:Immunoglobulin E