A prolongation of hepatic vascular exclusion by in situ hypothermic perfusion in dogs.
In situ hypothermic hepatic perfusion was performed in dogs to explore whether the time limit of hepatic vascular exclusion could be prolonged. During hepatic vascular exclusion, hepatic hypothermic perfusion was performed via portal vein using various perfusates under active bypass from the portal vein and infrahepatic inferior vena cava area to the superior vena cava system. Dogs receiving hepatic hypothermic perfusion for 1 hour died when given Ringer's solution but survived more than 7 days when given Euro-Collins' and University of Wisconsin solutions. Although dogs tolerated 2 hours of hepatic hypothermic perfusion when give University of Wisconsin solution, all dogs died by 2 hours of hepatic hypothermic perfusion when given Euro-Collins' solution. The hepatic energy charge and arterial ketone body ratio of dogs that died were significantly lower than for those that survived. This suggests that the regimen of hepatic hypothermic perfusion with University of Wisconsin solution is able to maintain the energy metabolism of the liver under hepatic vascular exclusion for prolonged periods, hence, its possible clinical application.
Takeuchi
T
T
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Egawa
H
H
Yamaoka
Y
Y
Taki
Y
Y
Ueda
J
J
Konishi
Y
Y
Yamamoto
N
N
Kagawa
R
R
Washida
M
M
Okamoto
R
R
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003250:Constriction; D004285:Dogs; D005260:Female; D005978:Glutathione; D006982:Hypertonic Solutions; D007036:Hypothermia, Induced / Q000379:methods*; D007328:Insulin; D007552:Isotonic Solutions; D007657:Ketone Bodies / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000098:blood supply / Q000378:metabolism / Q000601:surgery*; D008297:Male; D019188:Organ Preservation Solutions; D010477:Perfusion / Q000379:methods*; D011887:Raffinose; D000077331:Ringer's Solution; D012996:Solutions; D013997:Time Factors; D014021:Tissue Preservation
C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D007552:Isotonic Solutions; D007657:Ketone Bodies; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D000077331:Ringer's Solution; D007770:L-Lactate Dehydrogenase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose