title: The effect of atherogenic infusions of the triglyceride-rich, lipid emulsion, Lipofundin-S, on the in vitro growth characteristics of rat aortic smooth muscle cells.
abstract: This study shows that arterial smooth muscle cells (SMC) isolated from rats receiving atherogenic doses of the lipid emulsion, Lipofundin-S, alter their in vitro growth properties. Compared to cells from control animals, SMC isolated from Lipofundin-S-infused rats show a reduction in both saturation density and response to increasing serum concentrations, without a change in the baseline proliferation. Also, SMC isolated from lipid-treated animals and grown for five days in the presence of 30, 150, or 300 pg/ml estradiol show a 30% increase in growth vs. cells from controls. Epinephrine at 1 microM stimulates growth in SMC from control rats, while causing no growth enhancement over five days in cells from lipid-infused animals. Thus, atherogenic infusions of Lipofundin-S into rats cause phenotypic changes in arterial SMC which can be passed to successive cell generations in vitro.
authors.affiliation: Department of Medicine, Nassau County Medical Center, East Meadow, New York 11554.
authors.forename: C F
authors.initials: CF
authors.lastname: Saladino
authors.forename: Q
authors.initials: Q
authors.lastname: Yeh
authors.forename: E
authors.initials: E
authors.lastname: Zimmer
authors.forename: E A
authors.initials: EA
authors.lastname: Jonas
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000187:drug effects; D001161:Arteriosclerosis / Q000139:chemically induced*; D001790:Blood Physiological Phenomena; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004338:Drug Combinations; D004837:Epinephrine / Q000502:physiology; D004958:Estradiol / Q000502:physiology; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage* / Q000737:chemistry; D005990:Glycerol / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D010743:Phospholipids / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D013024:Soybean Oil / Q000008:administration & dosage* / Q000737:chemistry; D014280:Triglycerides / Q000032:analysis*
chemical_list: D004338:Drug Combinations; D005217:Fat Emulsions, Intravenous; D010743:Phospholipids; D014280:Triglycerides; D004958:Estradiol; C046535:Lipofundin S; D013024:Soybean Oil; D005990:Glycerol; D004837:Epinephrine