A new essential gene of Saccharomyces cerevisiae, a defect in it may result in instability of nucleus.
We identified a new essential gene of Saccharomyces cerevisiae, designated NIN1 (nuclear integrity). The nin1-1 mutant cells showed temperature-sensitive growth, and their nuclei underwent deformation and eventually were broken down upon a temperature shift. When the nin1-1 cells growing at 25 degrees C were transferred to a nonpermissive temperature, the nucleus moved to the isthmus and cells with a large bud accumulated. Flow cytometry proved that G2 nuclei accumulated during incubation at a nonpermissive temperature. NIN1 is mapped on chromosome VI, 16 cM centromere-distal to PHO4. Cloning and sequencing of NIN1 revealed that this is a new gene. Disruption of NIN1 resulted in cell death; however, a dead spore clone contained about 30 dead cells, indicating that spores that received the disrupted nin1 allele divided approximately five times before their death. Western blotting using anti-Nin1 antibody reveals that NIN1 produces a doublet consisting of 32 and 34 kDA whose size is close enough to that calculated from the predicted amino acid sequence of Nin1.
Nisogi
H
H
Department of Fermentation Technology, Hiroshima University, Japan.
Kominami
K
K
Tanaka
K
K
Toh-e
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000032:analysis*; D005800:Genes, Fungal; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D046988:Proteasome Endopeptidase Complex; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature
D005656:Fungal Proteins; C074096:RPN12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D046988:Proteasome Endopeptidase Complex