Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated Staphylococcus aureus.
Toxic shock syndrome toxin (TSST) 1 was purified from ovine (TSST-ovine) and bovine (TSST-bovine) mastitis-associated Staphylococcus aureus. These toxins were previously reported to have molecular weights identical to that of human TSST-1. However, TSST-ovine was reported as having an isoelectric point (pI) of 8.5, whereas TSST-bovine has the same pI (7.2) as TSST-1. Nucleotide sequence analysis revealed that TSST-bovine was identical to TSST-1 and that TSST-ovine had 14 nucleotide differences that changed 9 amino acid residues. Only 1 nucleotide difference, at position 514, was predicted to cause an amino acid charge difference, as glutamic acid at position 132 of TSST-1 was changed to lysine in TSST-ovine. Like TSST-1, TSST-ovine was mitogenic, but unlike TSST-1, it was not pyrogenic, was unable to enhance endotoxic shock, and was unable to induce TSS in a rabbit model. Also, TSST-ovine was less reactive to certain monoclonal antibodies raised against TSST-1.
Lee
P K
PK
Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455-0312.
Kreiswirth
B N
BN
Deringer
J R
JR
Projan
S J
SJ
Eisner
W
W
Smith
B L
BL
Carlson
E
E
Novick
R P
RP
Schlievert
P M
PM
D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins; D001483:Base Sequence; D015153:Blotting, Western; D002417:Cattle; D004269:DNA, Bacterial / Q000737:chemistry; D004768:Enterotoxins / Q000737:chemistry / Q000235:genetics*; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D008413:Mastitis / Q000382:microbiology / Q000662:veterinary*; D008414:Mastitis, Bovine / Q000382:microbiology*; D008969:Molecular Sequence Data; D011817:Rabbits; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D012772:Shock, Septic / Q000209:etiology; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013211:Staphylococcus aureus; D018089:Superantigens
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal