Subunit structure of calgranulins A and B obtained from sputum, plasma, granulocytes and cultured epithelial cells.
The calcium-binding proteins calgranulins A and B co-purified with an elastase-specific inhibitor after the affinity and cation-exchange chromatography of the perchloric acid-soluble fraction of pooled sputum collected from patients with chronic obstructive pulmonary disease (Sallenave, J.-M. and Ryle, A.P. (1991) Biol. Chem. Hoppe-Seyler 372, 13-21). The calgranulins were separated from the inhibitor by reverse-phase FPLC. Protein blot analysis of the calgranulin fraction in the absence of reducing agent revealed a band of 25 kDa corresponding to the disulphide-bonded heterodimerization of the two monomer components. Similar results were obtained from the immunoprecipitation and protein blot analysis of plasma, granulocytes and cultured epithelial cells. This implies that the calgranulins exist in the heterodimeric form in secretions in vivo. Their association with pancreatic elastase during the affinity chromatography stage of purification implicates them in the tissue destruction elicited by the inflammatory response in chronic obstructive pulmonary diseases.
Longbottom
D
D
MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Sallenave
J M
JM
van Heyningen
V
V
D002118:Calcium / Q000737:chemistry; D002135:Calcium-Binding Proteins / Q000097:blood / Q000737:chemistry*; D040501:Calgranulin A; D040502:Calgranulin B; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000503:physiopathology; D004848:Epithelium / Q000737:chemistry*; D006098:Granulocytes / Q000737:chemistry*; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight; D011233:Precipitin Tests; D013183:Sputum / Q000737:chemistry*
D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D046911:Macromolecular Substances; D002118:Calcium