title: Inhibition of interleukin 1 induced chondrocyte protease activity by a corticosteroid and a nonsteroidal antiinflammatory drug.
abstract: We report that administration of the corticosteroid, methylprednisolone (PRED) inhibited interleukin 1 (IL-1) induction of chondrocyte caseinolytic activity (25-55%) and collagenolytic activity (15-24%). The nonsteroidal antiinflammatory drug (NSAID), naproxen (NAP) had no effect on either enzyme activity over a therapeutic range (7-30 micrograms/ml) but at 120 micrograms/ml inhibited IL-1 induced caseinolytic and collagenolytic activity by 17 and 19%, respectively. However, PRED (2 micrograms/ml) in combination with NAP (30 micrograms/ml) significantly increased the inhibition of caseinolytic activity (p less than 0.001) compared to that observed with PRED (2 micrograms/ml) alone. The suppression of IL-1 induced collagenolytic activity noted with PRED in combination with NAP did not exceed that observed with PRED alone.
authors.affiliation: Division of Rheumatology, UCSF.
authors.forename: N E
authors.initials: NE
authors.lastname: Lane
authors.forename: R J
authors.initials: RJ
authors.lastname: Williams
authors.forename: D J
authors.initials: DJ
authors.lastname: Schurman
authors.forename: R L
authors.initials: RL
authors.lastname: Smith
mesh_terms: D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000166:cytology / Q000201:enzymology*; D002364:Caseins / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology*; D008775:Methylprednisolone / Q000494:pharmacology; D009288:Naproxen / Q000494:pharmacology; D011458:Prostaglandins E / Q000097:blood; D011480:Protease Inhibitors / Q000494:pharmacology*
chemical_list: D000305:Adrenal Cortex Hormones; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002364:Caseins; D007375:Interleukin-1; D011458:Prostaglandins E; D011480:Protease Inhibitors; D009288:Naproxen; D003094:Collagen; D010450:Endopeptidases; D008775:Methylprednisolone