A study of embolizing materials for chemo-embolization therapy of hepatocellular carcinoma: embolic effect of cisplatin albumin microspheres using chitin and chitosan in dogs, and changes of cisplatin content in blood and tissue.
Hepatic artery of dogs was embolized with cisplatin (CDDP) albumin microspheres containing chitin and chitosan to investigate the in vivo CDDP release kinetics from CDDP albumin microspheres, the CDDP cumulative characteristics in the liver, and the influence of microsphere administration on hepatic tissue. Results showed that changes in blood CDDP content were dependent on CDDP albumin microsphere type and that release kinetics were better sustained when chitin was added to the microspheres or when the microspheres were treated with chitosan. In particular, the administration of CDDP in the chitin-containing CDDP chitosan albumin microspheres showed a blood CDDP content of approximately 0.26 micrograms Pt/ml 14 d after administration. The administration of chitin-containing or chitosan treated CDDP microspheres showed a CDDP content in the hepatic tissue of 0.14 to 0.23 micrograms Pt/g 28 d after administration. They also showed better control of CDDP release than those without chitin or chitosan treatment. No CDDP influence on hepatic tissue was observed. We conclude that, even in vivo, chitin and chitosan are effective embolic materials.
Nishioka
Y
Y
Department of Pharmacy, Kochi Medical School Hospital, Japan.
Kyotani
S
S
Okamura
M
M
Miyazaki
M
M
Sakamoto
Y
Y
Morita
M
M
Okazaki
K
K
Ohnishi
S
S
Yamamoto
Y
Y
Ito
K
K
D000818:Animals; D016461:Chemoembolization, Therapeutic; D002686:Chitin / Q000031:analogs & derivatives; D048271:Chitosan; D002945:Cisplatin / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D004285:Dogs; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000628:therapy*; D008863:Microspheres
D002686:Chitin; D048271:Chitosan; D002945:Cisplatin