Two forms of G(o) type G proteins: identification and distribution in various rat tissues and cloned cells.
A G(o) type G protein distinct from the major species of G(o) was recently isolated from bovine brain and designated G(o)*. The cDNAs encoding two forms of mammalian G(o) alpha were also isolated and designated GoA alpha and GoB alpha. To recognize two forms of G(o) type G proteins, we raised antibodies in rabbits against two peptides with sequences found only in the respective proteins of murine GoA alpha (SNTYEDAAAYIQTQF) and GoB alpha (TEAVAHIQGQYWSK). Purified anti-GoA alpha antibodies reacted with the major species of G(o) alpha purified from bovine and rat brain, whereas anti-GoB alpha antibodies reacted only with rat G(o)*alpha, but not with the major species of G(o) alpha or bovine G(o)*alpha. These results indicate that the major species of G(o) alpha is encoded by GoA alpha cDNA and G(o)*alpha is encoded by GoB alpha cDNA. Using these antibodies, the distribution of GoA and GoB was studied in various rat tissues and cloned cells. Both GoA and GoB were present in many tissues, but their distribution in peripheral tissues was distinct. GoA alpha seemed to associate mainly with neural tissues. On the other hand, relatively high concentrations of GoB alpha were present in the brain, pituitary gland, adipose tissue, lung, and testis. The concentrations of both GoA and GoB in the brain increased during ontogenic development, but the increase in GoB was observed at a later age. Both GoA and GoB were found in such cloned cells as PC12, NG108-15, C6, GA-1, G8, and 3T3-L1 cells. Treatment of PC12 cells with nerve growth factor caused the extension of neuron-like processes and the increase in the level of GoA, but not in the level of GoB.
Asano
T
T
Department of Biochemistry, Institute for Developmental Research, Aichi Prefectural Colony, Japan.
Morishita
R
R
Kato
K
K
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D001923:Brain Chemistry; D002417:Cattle; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002999:Clone Cells; D005576:Colforsin / Q000494:pharmacology; D004247:DNA / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D005910:Glioma / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D006825:Hybridomas / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D015151:Immunoblotting; D008168:Lung / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016716:PC12 Cells; D010902:Pituitary Gland / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000378:metabolism / Q000473:pathology
D004273:DNA, Neoplasm; D005576:Colforsin; D004247:DNA; D019204:GTP-Binding Proteins