An osteosarcoma cell line with hypodiploid chromosome number and complex structural anomalies.
Giemsa-banding analysis performed on a newly established metastatic osteosarcoma cell line (SP-388) that was passaged through the nude mouse revealed a hypodiploid stemline number of 39 chromosomes. This cell line showed both numerical and structural abnormalities, including the abnormally banded region in certain marker chromosomes. Chromosomes 3, 15, 17 and the X were the most frequently absent elements of this tumor. Segments of chromosomes 3, 15 and X could not be identified even in rearranged chromosomes (Ml to M15). We suggest that more cases of osteosarcoma should be studied so that specific primary and secondary chromosomal anomalies can be identified in human osteosarcoma.
Dhaliwal
M K
MK
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Jaffe
N
N
Giovanella
B
B
Pathak
S
S
D000818:Animals; D002869:Chromosome Aberrations; D004171:Diploidy; D051379:Mice; D012516:Osteosarcoma / Q000235:genetics*; D014407:Tumor Cells, Cultured