Evaluation of 2-(2-thiophenecarboxy)benzoic acid and related active metabolites in biological samples.
This paper describes a quantitative assay of 2-(2-thiophenecarboxy)benzoic acid and its systemic metabolites, namely salicylic acid and thiophenecarboxylic acid, by high-performance liquid chromatography with ultraviolet detection at 254 nm. Analytes were extracted from acidified samples with tert.-butylmethyl ether and separated with an RP-18 column (150 mm x 3 mm I.D., 5 microns particle size) with a mixture of 0.01 M potassium phosphate and methanol (70:30, v/v) at pH 3.1. The method proved to have the validation required for pharmacokinetic investigations in animals and humans.
Lucarelli
C
C
Istituto Superiore di Sanità, Rome, Italy.
Pelloso
R
R
Bruno
G
G
La Rosa
C
C
Belliardo
F
F
D000818:Animals; D001241:Aspirin / Q000493:pharmacokinetics; D001565:Benzoates / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D002853:Chromatography, Liquid; D005260:Female; D006863:Hydrogen-Ion Concentration; D008297:Male; D009288:Naproxen / Q000097:blood / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D012459:Salicylates / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D020156:Salicylic Acid; D013056:Spectrophotometry, Ultraviolet; D013876:Thiophenes / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D014018:Tissue Distribution
D001565:Benzoates; D012459:Salicylates; D013876:Thiophenes; D009288:Naproxen; C074115:2-(2-thiophenecarboxy)benzoic acid; D020156:Salicylic Acid; D001241:Aspirin