title: Downregulation of messenger RNA levels for ribosomal proteins in differentiating HL-60 cells.
abstract: After differentiation induction in HL-60 cells by treatment with retinoic acid, phorbol ester, or dimethyl sulfoxide strong downregulation of the steady state mRNA level of the putative protein No. 3 of the large ribosomal subunit (rpL3) was observed. Downregulation was also observed in other hemopoietic human cell lines, although to a lesser extent. Four ribosomal protein mRNAs were compared in their degree of downregulation after differentiation induction or actinomycin D treatment. The comparatively fast response of rpL3 mRNA observed could indicate a regulatory function of rpL3 protein.
authors.affiliation: GSF-Institut für Experimentelle Hämatologie, München, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Mailhammer
authors.forename: H
authors.initials: H
authors.lastname: Szöts
authors.forename: J
authors.initials: J
authors.lastname: Bönisch
authors.forename: P
authors.initials: P
authors.lastname: Dörmer
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D015536:Down-Regulation; D006412:Hematopoietic Stem Cells; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins / Q000032:analysis / Q000235:genetics*; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; C000716547:RPL3 protein, human; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins; D014212:Tretinoin