title: Metacyclic form-specific variable surface glycoprotein-encoding genes of Trypanosoma (Nannomonas) congolense.
abstract: A complementary DNA expression library in phage lambda gt11 was synthesized using mRNA from in vitro-produced metacyclic forms of a clone of Trypanosoma (Nannomonas) congolense. The unamplified library was screened with antiserum from a goat immune to infection with metacyclic (m)-forms of T. congolense ILRAD Nannomonas antigen repertoire 2(ILNaR2). Of the 100 antiserum-reactive phage clones identified, 22 were analyzed further: 21 of the clones contained overlapping portions of a single transcript, while one other contained a different transcript. Northern blot analyses indicated that the sequences contained in the clones were transcribed only by m-forms of ILNaR2. Immunological and sequence analyses indicated that the two different cloned sequences encode m-form-specific variable surface glycoproteins.
authors.affiliation: International Laboratory for Research on Animal Diseases (ILRAD), Nairobi, Kenya.
authors.forename: Y
authors.initials: Y
authors.lastname: Eshita
authors.forename: T
authors.initials: T
authors.lastname: Urakawa
authors.forename: H
authors.initials: H
authors.lastname: Hirumi
authors.forename: W R
authors.initials: WR
authors.lastname: Fish
authors.forename: P A
authors.initials: PA
authors.lastname: Majiwa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014348:Trypanosoma congolense / Q000235:genetics*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics*
chemical_list: D000953:Antigens, Protozoan; D012333:RNA, Messenger; D014643:Variant Surface Glycoproteins, Trypanosoma; D004247:DNA