The regulation of translation by the 5' untranslated region of Xenopus c-myc I mRNA during early development.
The translational efficiency of chloramphenicol acetyltransferase (CAT) mRNA containing 5' untranslated region (UTR) sequences of Xenopus c-myc I mRNA was examined in the Xenopus oocyte and early embryo. In contrast to their reduced translation in the oocyte, CAT mRNAs containing 5' UTR sequences located between the c-myc I P0 and P2 promoters translate as efficiently as CAT mRNA controls when injected into early embryos. This pattern of differential regulation of c-myc I translation is similar to that observed for mouse c-myc 5' UTR-containing CAT transcripts during Xenopus oogenesis and early embryogenesis [Lazarus, P., Parkin, N. & Sonenberg, N. (1988). Oncogene, 3, 517-521]. Oocyte-specific translational inhibition was not observed for CAT mRNAs containing c-myc I 5' UTR sequences located 3' of the P2 promoter. No significant alteration in c-myc I 5' UTR/CAT mRNA stability was observed in the oocyte as compared with CAT mRNA controls. The significance of this promoter-dependent translational regulation with respect to c-myc expression and early Xenopus development is discussed.
Lazarus
P
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Department of Biochemistry, McGill University, Montreal, Canada.
D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005786:Gene Expression Regulation; D009865:Oocytes / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D016271:Proto-Oncogene Proteins c-myc / Q000502:physiology*; D012333:RNA, Messenger / Q000502:physiology*; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000235:genetics* / Q000254:growth & development*
C489428:Myc protein, mouse; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D029867:Xenopus Proteins; C069483:c-Myc1 protein, Xenopus; D015500:Chloramphenicol O-Acetyltransferase