Chromosomal in situ hybridization with double-labeled DNA: signal amplification at the probe level.
A double-labeling approach was applied to nonisotopic in situ hybridization with individual cosmid and plasmid clones, using digoxigenin or biotin as label and a combination of two separate enzymatic labeling methods. Probe labeling was achieved by nick translation, followed by tailing of the probe by terminal deoxynucleotidyl transferase. The double-labeling method, in conjunction with an improved detection protocol, provides for a higher signal intensity than that obtainable with single-labeled probes.
Scherthan
H
H
Division of Human Biology and Human Genetics, University of Kaiserslautern, FRG.
Köhler
M
M
Vogt
P
P
von Malsch
K
K
Schweizer
D
D
D001710:Biotin; D002875:Chromosomes / Q000378:metabolism; D003360:Cosmids / Q000235:genetics; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D015342:DNA Probes / Q000378:metabolism*; D004076:Digoxigenin; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis / Q000235:genetics
D015342:DNA Probes; D001710:Biotin; D004253:DNA Nucleotidylexotransferase; D016650:Fluorescein-5-isothiocyanate; D004076:Digoxigenin