A procedure for parallel isolation of white blood cells, granulocyte and purified neutrophil suspensions from the peripheral blood of cattle.
A rapid method is described for parallel isolation of white blood cells, granulocytes and purified neutrophils from peripheral blood of normal cattle. The mean recovery (+/- S.D.) of white blood cells, granulocytes and purified neutrophils isolated from peripheral blood of 13 cows was 66.4 +/- 12.6%, 68.7 +/- 20.0% and 38.0 +/- 20.9%, respectively. The mean purity of the isolated granulocyte and neutrophil suspensions was 94.0 +/- 3.8% and 95.0 +/- 6.0%, respectively. Viability of isolated cells was more than 97%.
Kremer
W D
WD
Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, University of Utrecht, Netherlands.
Noordhuizen-Stassen
E N
EN
Henricks
P A
PA
van der Vliet
H
H
D000818:Animals; D002417:Cattle / Q000097:blood*; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D007962:Leukocytes / Q000166:cytology*; D009504:Neutrophils / Q000166:cytology*; D012680:Sensitivity and Specificity