Distribution of IGF-I mRNA and IGF-I binding sites in the rat kidney.
In the present study we have investigated the distribution of IGF-I mRNA and IGF-I binding sites in the rat kidney. The distribution of IGF-I mRNA was investigated using a simple and sensitive non-radioactive in situ hybridisation technique based on probe labelling with digoxigenin labelled-UTP followed by detection with conventional immunocytochemical techniques. IGF-I mRNA was found predominantly in medullary collecting ducts and sparsely in cortical collecting duct cells. In addition IGF-I mRNA was expressed in scattered proximal tubular cells in the cortex and in cells confined to the glomerular tuft. IGF-I binding sites were studied using radiolabelled IGF-I and conventional autoradiographical techniques on tissue sections. It was found that IGF-I binding sites were widely distributed throughout the entire kidney and that the specific binding was highest in the inner medulla. These findings add further complexity to the understanding of IGF-I production and action on renal structures.
Matejka
G L
GL
Department of Histology, University of Göteborg, Sweden.
Eriksson
P S
PS
Carlsson
B
B
Jennische
E
E
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D015152:Blotting, Northern; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000737:chemistry* / Q000378:metabolism; D007672:Kidney Cortex / Q000737:chemistry / Q000378:metabolism; D007679:Kidney Medulla / Q000737:chemistry / Q000378:metabolism; D007684:Kidney Tubules / Q000737:chemistry / Q000378:metabolism; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains
D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I