title: Flow cytometric and molecular analysis of proliferative activity and DNA content in neuroblastoma: presence of stationary cells in S-phase.
abstract: Twenty-nine previously untreated neuroblastomas were analyzed for DNA content and percentage of cells in S-phase, both determined by flow cytometry, and N-myc oncogene copy number. Twelve of them were also tested for histone H3 transcript levels as a marker of actual proliferative activity. A significantly higher S-phase fraction was associated with advanced stages of disease, unfavorable (i.e., near-diploid and near-tetraploid) DNA content, and N-myc amplification. The occurrence of six tumors with a remarkable (greater than or equal to 10%) S-phase fraction but lacking histone H3 transcripts suggests the presence of stationary S-phase cells in neuroblastoma.
authors.affiliation: Department of Pediatrics, University La Sapienza, Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Dominici
authors.forename: A
authors.initials: A
authors.lastname: Negroni
authors.forename: A
authors.initials: A
authors.lastname: Romeo
authors.forename: H
authors.initials: H
authors.lastname: McDowell
authors.forename: A
authors.initials: A
authors.lastname: Padula
authors.forename: S
authors.initials: S
authors.lastname: Pucci
authors.forename: C
authors.initials: C
authors.lastname: Cappelli
authors.forename: M A
authors.initials: MA
authors.lastname: Castello
authors.forename: G
authors.initials: G
authors.lastname: Raschellà
mesh_terms: D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009447:Neuroblastoma / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D016196:S Phase
chemical_list: D004273:DNA, Neoplasm; D012333:RNA, Messenger