title: Tumour necrosis factor alpha restores granulomas and induces parasite egg-laying in schistosome-infected SCID mice.
abstract: Schistosomiasis (bilharzia) is a parasitic disease caused by several species of schistosome worms (blood flukes). The key pathogenic event in this disease is the formation of granulomas around schistosome eggs trapped in portal venules of the liver. Granulomas are a distinctive form of chronic inflammation characterized by localized aggregation of activated macrophages around an inciting stimulus. Each granuloma evolves to form a fibrous scar; in schistosomiasis, the result is widespread hepatic fibrosis and portal hypertension. To identify the specific immune signal molecules necessary for granuloma formation, we studied schistosome infections in severe combined immunodeficient (SCID) mice, which have normal macrophages but lack functional B or T lymphocytes. Here we report that the immunoregulatory cytokine tumour necrosis factor alpha is necessary and sufficient to reconstitute granuloma formation in schistosome-infected SCID mice. Moreover, we find that the parasitic worms require tumour necrosis factor alpha for egg-laying and for excretion of eggs from the host. The implication of this latter result is that the parasite has adapted so successfully to its host that it uses a host-derived immunoregulatory protein as a signal for replication and transmission.
authors.affiliation: Department of Pathology, University of California, San Francisco 94143-0506.
authors.forename: P
authors.initials: P
authors.lastname: Amiri
authors.forename: R M
authors.initials: RM
authors.lastname: Locksley
authors.forename: T G
authors.initials: TG
authors.lastname: Parslow
authors.forename: M
authors.initials: M
authors.lastname: Sadick
authors.forename: E
authors.initials: E
authors.lastname: Rector
authors.forename: D
authors.initials: D
authors.lastname: Ritter
authors.forename: J H
authors.initials: JH
authors.lastname: McKerrow
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004305:Dose-Response Relationship, Drug; D005260:Female; D006099:Granuloma; D007106:Immune Sera; D015847:Interleukin-4 / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D008099:Liver / Q000187:drug effects / Q000469:parasitology* / Q000473:pathology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D010058:Oviposition / Q000187:drug effects*; D012550:Schistosoma mansoni / Q000502:physiology*; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000494:pharmacology*
chemical_list: D000911:Antibodies, Monoclonal; D007106:Immune Sera; D015848:Interleukin-5; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4