[Effects of Sino-Japanese herbs in the family Umbelliferae on the hepatic drug metabolizing enzymes and lipid peroxidation in rats].
Eight kinds of hot water extracts (HWE) and 2 kinds of distanninized fractions (DTF) from Umbelliferae herbs were prepared. The effects of HWE and DTF on rat hepatic lipid peroxidation (LPO), aminopyrine N-demethylase (APD) and aniline hydroxylase (ANH) activities were examined in vitro. The APD activity was inhibited by HWE, DTF from Byakushi and HWE from Uikyo, Zenko, Toki, Senkyu, Bofu and Saiko, respectively, whereas DTF from Uikyo caused no significant effect. The ANH activity was inhibited by HWE, DTF from Byakushi, Uikyo and HWE from Bofu, Zenko, Hokushajin and Toki, respectively. LPO was inhibited by HWE from Zenko, Byakushi and Senkyu, but increased by Saiko, Uikyo, Bofu and Hokushajin. The results of Uikyo DTF suggests that the components of Uikyo that altered the activity of APD and ANH are different respectively. Marked inhibitions on the activity of APD and ANH caused by Byakushi indicate that Byakushi might affect the activity of hepatic drug metabolizing enzymes in vivo.
Mayanagi
M
M
Department of Pharmacology, School of Medicine, Showa University, Tokyo, Japan.
Nakayama
S
S
Oguchi
K
K
D000633:Aminopyrine N-Demethylase / Q000378:metabolism*; D000815:Aniline Hydroxylase / Q000378:metabolism*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D004365:Drugs, Chinese Herbal; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase