Effects of UV-irradiation upon Venezuelan equine encephalomyelitis virus.
Venezuelan equine encephalomyelitis virus labelled with [14C]aminoacids or [3H]uridine was purified and UV-irradiated. The irradiation led to the formation of uracil photodimers and the covalent linking of the nucleocapsid protein C to virion RNA. The inactivation of infectivity correlated with the formation of uracil dimers, whereas the RNA-protein links were formed at much higher doses of UV irradiation. The analysis of covalent RNA-protein complexes suggests that a fairly large fraction (at least one third) of the whole content of C protein is able to participate in the formation of UV-induced links, suggesting extensive contacts of RNA with protein with the nucleocapsid.
Smirnov YuA
D. I. Ivanovsky Institute of Virology, Academy of Medical Sciences of the U.S.S.R., Moscow.
Kapitulez
S P
SP
Kaverin
N V
NV
D000818:Animals; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects*; D005347:Fibroblasts; D011740:Pyrimidine Dimers / Q000737:chemistry / Q000528:radiation effects; D012367:RNA, Viral / Q000737:chemistry / Q000528:radiation effects; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000737:chemistry / Q000528:radiation effects; D014775:Virus Activation / Q000528:radiation effects*
D011740:Pyrimidine Dimers; D012367:RNA, Viral; D014764:Viral Proteins