title: A murine monoclonal antibody directed against the Gerbich 3 blood group antigen.
abstract: A murine monoclonal antibody (NaM19-3C4, IgG1, Kappa) was produced from splenocytes of mice immunized with red blood cells. The antibody agglutinated untreated Ge:2,3,4 and Ge:-2,3,4 erythrocytes in indirect antiglobulin test but failed to agglutinate trypsin-treated cells. Gerbich-negative erythrocyte of the Leach- (Ge:-2,-3,-4) and of the Gerbich- (Ge:-2,-3,4) types were not recognized by the antibody. Immunoblotting experiments showed that the antibody bound to glycophorins C and D from control erythrocytes and to the abnormal glycophorin C identified in the Gerbich-negative cells of the Yussef type (Ge:-2,3,4). No binding to the altered glycophorin C from Ge:-2,-3,4 erythrocytes was observed, indicating that the antibody specifically recognized the Ge:3 epitope localized within residues 40-50 of glycophorin C.
authors.affiliation: Centre Régional de Transfusion Sanguine, Nantes, France.
authors.forename: M J
authors.initials: MJ
authors.lastname: Loirat
authors.forename: A
authors.initials: A
authors.lastname: Gourbil
authors.forename: Y
authors.initials: Y
authors.lastname: Frioux
authors.forename: J Y
authors.initials: JY
authors.lastname: Muller
authors.forename: D
authors.initials: D
authors.lastname: Blanchard
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity / Q000276:immunology; D001789:Blood Group Antigens / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D007519:Isoantigens / Q000032:analysis*; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D007519:Isoantigens