Transplantation of cultured thymic fragments in congenitally athymic and euthymic rats. Culture with deoxyguanosine or cyclosporin A does not influence the histologic characteristics and outcome after transplantation in syngeneic and allogeneic combinations.
Cultured thymic fragments (CTF) from WAG/CPB (RT1u) and DA/01a (RT1a) rats were prepared in the presence or absence of 2'deoxyguanosine or cyclosporin A, and subsequently transplanted under the kidney capsule of congenitally athymic and euthymic WAG/CPB recipients. The rationale of the culture supplements was that these may affect the disappearance of medullary dendritic cells, with subsequent induction of allotolerance. However, the immunohistology of the CTF showed more RT1 class II-positive cells than keratin-positive cells, indicative of the maintenance of dendritic cells. Grafts in athymic animals showed the recovery of the original thymic architecture within 6 weeks after transplantation. The influx of host-derived lymphocytes was accompanied by an influx of dendritic cells in the medulla-like area and macrophages in the cortex. A similar recovery was observed for syngeneic CTF in euthymic recipients. In addition lymphocytic infiltration was seen in the connective tissue surrounding the epithelial areas. Allogeneic grafts in euthymic animals were rejected within 3 weeks after transplantation. This outcome of the transplanted CTF under different conditions was not affected by the supplementation of the thymic culture before transplantation with 2'deoxyguanosine or cyclosporin A. We conclude that there is no tolerance induction after transplantation in euthymic allogeneic rats of CTF prepared in the presence of 2'deoxyguanosine. This conclusion is in contrast to data in the mouse, which may be explained by the maintenance of dendritic cells during culture. A chimaeric state of donor-derived epithelium and host-derived dendritic cells is obtained by transplantation of allografts in athymic rats.
Martín-Fontecha
A
A
Department of Pathology, University Hospital, Utrecht, The Netherlands.
Broekhuizen
R
R
de Heer
C
C
Zapata
A
A
Schuurman
H J
HJ
D000704:Analysis of Variance; D000818:Animals; D046508:Culture Techniques / Q000379:methods*; D016572:Cyclosporine / Q000276:immunology*; D003849:Deoxyguanosine / Q000276:immunology*; D007108:Immune Tolerance / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D011923:Rats, Nude; D013950:Thymus Gland / Q000166:cytology / Q000637:transplantation*; D014184:Transplantation, Homologous / Q000276:immunology*; D014185:Transplantation, Isogeneic / Q000276:immunology*
D016572:Cyclosporine; D003849:Deoxyguanosine