title: Migration of astrocytes transplanted to the midbrain of neonatal rats.
abstract: Previous studies have indicated that transplanted astrocytes are able to survive, express glial fibrillary acidic protein (GFAP), and migrate in the host brain, and that the pattern and speed of astrocyte migration is largely determined by the location of the graft. We examine here the pattern of astrocyte migration in the midbrain by transplanting CD-1 mouse corpus callosum (P2-3) into the midbrain of neonatal rats. The location of the grafts and the distribution of donor astrocytes were assessed by using a monoclonal antibody (anti-M2) specific for mouse astrocytes. A characteristic donor astrocyte distribution was seen. The highest density of cells was in the region of the substantia nigra (SN); lower numbers were found in the medial geniculate nucleus (MGN). Donor astrocytes were also found in the superior colliculus (SC) and central gray region, but only when the body of a graft was located nearby. [3H]thymidine studies showed that the concentrations of donor astrocytes in the SN were not the result of high levels of mitotic activity: all indications were that the proportion of dividing donor cells closely matched that of host glia. The pattern of astrocyte migration in the midbrain did not follow the course established by radial glia and was not influenced by axonal degeneration in the SC after removal of eyes. Moreover, donor cells failed to migrate along the course of axonal outgrowth from co-grafted retinae. Reciprocally, axonal elongation from retinal grafts did not follow the pathway of astrocyte migration, thus suggesting that astrocyte migration and neuronal outgrowth follow different cues.
authors.affiliation: Department of Neurobiology, Anatomy, and Cell Science, University of Pittsburgh School of Medicine, Pennsylvania 15261.
authors.forename: H F
authors.initials: HF
authors.lastname: Zhou
authors.forename: R D
authors.initials: RD
authors.lastname: Lund
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000637:transplantation*; D016380:Brain Tissue Transplantation; D002455:Cell Division; D002465:Cell Movement; D002540:Cerebral Cortex / Q000166:cytology / Q000637:transplantation; D003337:Corpus Callosum / Q000166:cytology / Q000637:transplantation; D015353:Eye Enucleation; D016332:Fetal Tissue Transplantation; D006085:Graft Survival; D051379:Mice; D009474:Neurons / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D013378:Substantia Nigra / Q000502:physiology; D013681:Tegmentum Mesencephali; D014183:Transplantation, Heterologous; D016042:Transplantation, Heterotopic