Monoclonal antibody-based immunoassay of vitellogenin in the blood of male channel catfish (Ictalurus punctatus).
1. A monoclonal antibody to vitellogenin of channel catfish (Ictalurus punctatus) was made, and its specificity was demonstrated using Western blots of serum from female fish, estradiol-treated male fish, untreated male fish, vitellogenin purified by three different methods and egg extracts. 2. An enzyme-linked immunosorbent assay (ELISA), using this monoclonal antibody, detected vitellogenin in the plasma of 59 out of 60 untreated 17-24-month-old male channel catfish with a mean concentration of 338 micrograms/ml and a maximum concentration of 4240 micrograms/ml. 3. Vitellogenin levels in male channel catfish were unrelated to testicular stage, gonadosomatic index and month.
Goodwin
A E
AE
Department of Fisheries and Allied Aquacultures, Alabama Agricultural Experimental Station, Auburn University 36849.
Grizzle
J M
JM
Bradley
J T
JT
Estridge
B H
BH
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D007059:Ictaluridae; D008297:Male; D014819:Vitellogenins / Q000097:blood* / Q000276:immunology
D000911:Antibodies, Monoclonal; D014819:Vitellogenins