Synthetic trimer and tetramer of 3-beta-D-ribose-(1-1)-D-ribitol-5-phosphate conjugated to protein induce antibody responses to Haemophilus influenzae type b capsular polysaccharide in mice and monkeys.
Synthetic oligosaccharides derived from the capsular polysaccharide (PRP) of Haemophilus influenzae type b were conjugated to carrier proteins via a thioether linkage. Conjugates were made of trimeric and tetrameric ribose-ribitol-phosphate and tetanus toxoid or diphtheria toxin. All conjugates elicited anti-PRP antibody responses with an increasing immunoglobulin G/immunoglobulin M ratio in adult mice and monkeys. Trimer conjugates elicited lower anti-PRP antibody responses compared with tetramer conjugates. Adult monkeys responded equally well to the tetrameric oligosaccharide-tetanus toxoid conjugate as to the oligosaccharide-CRM197 conjugate (HbOC), which elicits protective levels of serum antibodies in human infants after two or three injections.
Peeters
C C
CC
Unit for Bacterial Vaccine Development and Pathogenesis Research, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Evenberg
D
D
Hoogerhout
P
P
Käyhty
H
H
Saarinen
L
L
van Boeckel
C A
CA
van der Marel
G A
GA
van Boom
J H
JH
Poolman
J T
JT
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D016667:Bacterial Capsules; D001428:Bacterial Vaccines / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology; D004167:Diphtheria Toxin / Q000276:immunology; D005260:Female; D018073:Haemophilus Vaccines; D006193:Haemophilus influenzae / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000032:analysis; D008252:Macaca fascicularis; D008297:Male; D051379:Mice; D009844:Oligosaccharides / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D004167:Diphtheria Toxin; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D007132:Immunoglobulin Isotypes; D009844:Oligosaccharides; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D013745:Tetanus Toxoid; C008984:polyribitol phosphate