Isolation of three novel Drosophila melanogaster genes containing repetitive sequences rich in GCN triplets.
Several cDNA and genomic clones were isolated from Drosophila melanogaster gene libraries by hybridization with a region of a mammalian gene that contains a simple repetitive sequence of six GCN repeats. One of the cDNA clones, E6, was completely sequenced and it was shown that it contains a region of 16 GCN repeats; these repeats encode a polyalanine stretch within a long open reading frame. The sequencing of three different genomic clones (A, B, and D) revealed that all the isolated Drosophila clones are similar to one another in a short region containing variable numbers of the GCN repeat. The genomic clone B was found to be the genomic counterpart of the cDNA clone E6. The other genomic clones, A and D, also hybridize with Drosophila cDNA clones at high stringency. These results indicate that the short GCN repetitive sequences, which we have named ala, are found within transcribed regions of the Drosophila genome. These Drosophila genes containing the ala repeat do not show significant sequence similarity to any presently known gene; we have named these novel genes ala-A, ala-B, and ala-D. The cDNA clone from gene ala-B was named ala-E6.
Magoulas
C
C
Department of Biology, University of Ottawa, Ont., Canada.
Hickey
D A
DA
D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004247:DNA; D004331:Drosophila melanogaster / Q000235:genetics*; D008322:Mammals; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004247:DNA