Mass spectrometric characterization of bovine chromaffin granule peptides related to chromogranin B.
Peptides were extracted from the lysate of isolated bovine chromaffin granules. Following reversed-phase HPLC purification, the fractions were analyzed by FAB/MS. The presence of methionine-enkephalin and leucine-enkephalin was indicated by their chromatographic retention time and by the m/z value of their protonated molecules. As to five new peptides related to chromogranin B, prominent protonated molecules were observed at m/z 1746, 1446, 1333, 977 and 901. Trypsinolysis resulted in a common loss of a component with mass 545, pointing to a structural relationship and a common precursor molecule. The peptide showing a (M+H)+ ion at m/z 1746 could be identified as a novel, recently reported, neuropeptide derived from chromogranin B, whereas the other peptides with (M+H)+ ions at m/z 1446, 1333, 977 and 901 could be characterized as smaller fragments of this peptide. Peptidase-guided sequence analysis and MS/MS analysis provided sequence information.
Dillen
L
L
Department of Pharmaceutical Sciences, University of Antwerp U.I.A., Belgium.
Boel
S
S
De Potter
W P
WP
Claeys
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000737:chemistry*; D053378:Chromogranin B; D002864:Chromogranins / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D014357:Trypsin
D053378:Chromogranin B; D002864:Chromogranins; D009479:Neuropeptides; D010446:Peptide Fragments; C068759:BAM 1745; D014357:Trypsin; D011761:Pyrrolidonecarboxylic Acid