title: Endothelin stimulates cardiac alpha- and beta- myosin heavy chain gene expression.
abstract: The effect of endothelin-1 on cardiac myosin heavy chain gene expression was examined using an isolated neonatal rat myocardial cell culture system. The effects of endothelin-1 on the expression of alpha- and beta- myosin heavy chain genes in the primary rat myocardial cell culture system were examined by S1 nuclease protection analysis. Endothelin-1 was found to stimulate both alpha- and beta- myosin heavy chain gene expression. The 5' flanking regions of both the alpha- and beta- myosin heavy chain gene promoters ligated to a reporter gene, chloramphenicol acetyltransferase, were used to study the effect of endothelin-1 on transcription. Myocardial cells treated with endothelin-1 increased the transcription rate of alpha- and beta- myosin heavy chain genes in a dose-dependent manner. Thus, the hypertrophic effect of endothelin-1 on cardiac myocytes involves augmentation of alpha- and beta- myosin heavy chain gene expression by increasing gene transcription.
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.
authors.forename: D L
authors.initials: DL
authors.lastname: Wang
authors.forename: J J
authors.initials: JJ
authors.lastname: Chen
authors.forename: N L
authors.initials: NL
authors.lastname: Shin
authors.forename: Y C
authors.initials: YC
authors.lastname: Kao
authors.forename: K H
authors.initials: KH
authors.lastname: Hsu
authors.forename: W Y
authors.initials: WY
authors.lastname: Huang
authors.forename: C C
authors.initials: CC
authors.lastname: Liew
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000494:pharmacology*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D015342:DNA Probes; D016232:Endothelins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D015500:Chloramphenicol O-Acetyltransferase; D009218:Myosins