title: Cancer cell motility-inhibitory protein in the Dunning adenocarcinoma model.
abstract: Cell motility has been associated with metastatic ability in the Dunning R3327 rat prostatic adenocarcinoma model. Cancer cell motility promoters but not inhibitors have been described by many investigators. Serum-containing and serum-free media conditioned by the nonmotile, nonmetastatic G Dunning subline inhibited the motility of the highly motile, highly metastatic MAT-LyLu subline. Motility inhibition by the G subline-conditioned serum-free media was lost upon heating to 100 degrees C and by treatment with trypsin. The motility inhibitory protein(s) had a molecular weight exceeding 50,000 as determined by diafiltration. G subline-conditioned RPMI 1640 contained several proteins with molecular weights of between approximately 53,000 and 116,000 when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. One of these bands may represent the first inhibitor of cancer cell motility identified.
authors.affiliation: Department of Surgery, University of North Carolina, Chapel Hill 27599-7235.
authors.forename: J L
authors.initials: JL
authors.lastname: Mohler
authors.forename: E N
authors.initials: EN
authors.lastname: Broskie
authors.forename: D J
authors.initials: DJ
authors.lastname: Ranparia
authors.forename: Y
authors.initials: Y
authors.lastname: Sharief
authors.forename: W B
authors.initials: WB
authors.lastname: Coleman
authors.forename: G J
authors.initials: GJ
authors.lastname: Smith
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry*; D000818:Animals; D002465:Cell Movement; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000737:chemistry / Q000302:isolation & purification*; D011471:Prostatic Neoplasms / Q000737:chemistry*; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D009363:Neoplasm Proteins