Expression of the polymorphic human DNA repair gene XRCC1 does not correlate with radiosensitivity in the cells of human head and neck tumor cell lines.
The human X-ray repair cross-complementing gene 1 (XRCC1) is highly polymorphic in the cells of human head and neck squamous cell carcinoma lines and in a variety of other human cell lines. We describe six patterns seen in Southern analysis of EcoRI-digested genomic DNA. Levels of XRCC1 mRNA vary in cells of human head and neck cell lines as seen in northern blots. Expression of this gene in cell culture does not correlate with radiobiological parameters, clinical outcome, or the DNA restriction fragment length polymorphisms.
Dunphy
E J
EJ
Department of Radiation and Cellular Oncology, Michael Reese/University of Chicago Center for Radiation Therapy, Illinois 60615.
Beckett
M A
MA
Thompson
L H
LH
Weichselbaum
R R
RR
D015152:Blotting, Northern; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics* / Q000528:radiation effects; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D011836:Radiation Tolerance / Q000235:genetics*; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D012334:RNA, Neoplasm