Percutaneous absorption of [14C]chlordane from soil.
The objective was to determine percutaneous absorption of chlordane in vitro and in vivo from soil into and through skin. The data are needed to calculate the absorbed dose of chlordane from soil, which is then used to assess the toxicity risk. Chlordane, an insecticide for which residues exist in soil, is restricted currently to use for termite control. Chlordane is highly lipophilic with little or no movement out of soil. Soil (Yolo County 65-California-57-8; 26% sand, 26% clay, 48% silt, 0.9% organic) was passed through 10-, 20-, and 48-mesh sieves. Soil then retained by 80-mesh was mixed with 14C-labeled chemical at 67 ppm. Acetone solutions were prepared for comparative analysis. Human cadaver skin was dermatomed to 500 microns and used in glass diffusion cells with human plasma as the receptor fluid (3 ml/h flow rate) for a 24-h skin application time. Chlordane concentration within skin from in vitro studies was 0.34 +/- 0.31% from soil and 10.8 +/- 8.2% from acetone vehicle (p less than .01). Individual variation from human skin sources was evident (p less than .008). Chlordane accumulation in human plasma receptor fluid was the same for soil (0.04 +/- 0.05%) and acetone (0.07% +/- 0.06%) formulations. Most of the remaining chlordane was recovered in the soap and water skin surface wash. In contrast, in vivo percutaneous absorption of chlordane in the rhesus monkey was the same for soil (4.2 +/- 1.8%) and acetone (6.0 +/- 2.8%) formulations (p = .29, nonsignificant). Multiple soap and water washings were necessary to remove chlordane from skin, suggesting that a single wash may not adequately remove all the chlordane.
Wester
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RC
Department of Dermatology, University of California School of Medicine, San Francisco 94143-0989.
Maibach
H I
HI
Sedik
L
L
Melendres
J
J
Liao
C L
CL
DiZio
S
S
D000287:Administration, Topical; D000818:Animals; D002706:Chlordan / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine; D046508:Culture Techniques; D005260:Female; D006801:Humans; D007275:Injections, Intravenous; D008253:Macaca mulatta; D012869:Skin Absorption; D012989:Soil Pollutants / Q000008:administration & dosage / Q000493:pharmacokinetics*
D012989:Soil Pollutants; D002706:Chlordan