The use of bovine anti-PMSG serum in beef cattle after PMSG-superovulation.
Thirteen beef cows were superovulated using 4,000 i.u. of pregnant mare serum gonadotrophin (PMSG) on days 9 to 14 of the estrous cycle, followed by two injections of 500 micrograms prostaglandin F2 alpha analogue (PGF2 alpha) 48 and 55 hrs later. Seven of them were injected intramuscularly with bovine anti-PMSG serum 12 hrs after the first signs of estrus. The remaining 6 cows were served as controls and received no antiserum. Peripheral blood concentrations of progesterone (P) and estradiol-17 beta (E2) were compared in relation to the superovulatory responses. The injection of anti-PMSG serum did not significantly affect the numbers of the corpora lutea (CL), the anovulatory follicles and the transferable embryos at 7 to 8 days after superovulatory estrus, but increased the ratio of embryos classified as excellent or good quality. Although the plasma P concentration showed no significant differences between the anti-PMSG-treated and control cows, the plasma E2 concentration displayed a characteristic difference, suppressing the second E2 peak in the anti-PMSG-treated cows. It is concluded that the use of bovine anti-PMSG serum for PMSG/PGF2 alpha-treated cows at 12 hrs after the beginning of the estrus improves the quality of embryos recovered, probably due to inhibition of high estrogenic environment following ovulation.
Nakajima
A
A
Aomori Prefectural Experiment Station of Animal Husbandry, Japan.
Hiraizumi
S
S
Onodera
K
K
Suzuki
H
H
Kudo
Y
Y
Domeki
I
I
D000818:Animals; D002417:Cattle / Q000502:physiology*; D003338:Corpus Luteum / Q000276:immunology; D004624:Embryo Transfer / Q000592:standards / Q000662:veterinary*; D004958:Estradiol / Q000097:blood; D005260:Female; D006064:Gonadotropins, Equine / Q000276:immunology* / Q000494:pharmacology; D007106:Immune Sera / Q000276:immunology*; D006080:Ovarian Follicle / Q000276:immunology; D011374:Progesterone / Q000097:blood; D013480:Superovulation / Q000187:drug effects / Q000276:immunology*
D006064:Gonadotropins, Equine; D007106:Immune Sera; D011374:Progesterone; D004958:Estradiol