Diverse mechanisms of plant resistance to cauliflower mosaic virus revealed by leaf skeleton hybridization.
Plants not hosts for cauliflower mosaic virus (CaMV) may prevent systemic CaMV infection by interfering with dissemination of infection through the plant or by preventing viral replication and maturation. Leaf skeleton hybridization allows distinction between these two barriers. The technique assesses the spatial distribution of CaMV in an inoculated leaf by hybridization of a skeleton of the leaf with a CaMV DNA probe. Leaves or leaflets of soybean, cucumber, peanut, tomato, lettuce, spinach, pepper, onion, wheat, maize and barley, inoculated with CaMV DNA or CaMV virions were processed for leaf skeleton hybridization either immediately after inoculation or two weeks thereafter. Autoradiographic images of soybean and cucumber skeletons had many dark spots suggesting that CaMV DNA replication and local spread had occurred. Images of onion leaf skeletons prepared two weeks after inoculation with CaMV DNA had fewer spots. To test whether these spots resulted from CaMV replication, DNA was extracted from inoculated onion leaves and analyzed by electrophoresis, blotting and hybridization. Molecules recovered two weeks after inoculation resembled those inoculated, indicating absence of replication. For the other species, we found no evidence of local spread of CaMV infections. Thus, many plant species resist systemic CaMV infection by preventing replication or local spread of CaMV, while others solely prevent systemic movement of infection.
Melcher
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Department of Biochemistry, Oklahoma State University, Stillwater.
Brannan
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CM
Gardner
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Essenberg
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RC
D015139:Blotting, Southern; D015342:DNA Probes; D004279:DNA, Viral; D009029:Mosaic Viruses / Q000235:genetics / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000276:immunology* / Q000382:microbiology; D014779:Virus Replication
D015342:DNA Probes; D004279:DNA, Viral