Repair of ultraviolet light damage in Saccharomyces cerevisiae as studied with double- and single-stranded incoming DNAs.
Purified double- and single-stranded DNAs of the autonomously replicating vector M13RK9-T were irradiated with ultraviolet light (UV) in vitro and introduced into competent whole cells of Saccharomyces cerevisiae. Incoming double-stranded DNA was more sensitive to UV in excision repair-deficient rad2-1 cells than in proficient repair RAD+ cells, while single-stranded DNA exhibited high sensitivity in both host cells. The results indicate that in yeast there is no effective rescue of UV-incoming single-stranded DNA by excision repair or other constitutive dark repair processes.
Keszenman-Pereyra
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D
Department of Physics, Rikkyo University, Tokyo, Japan.
Hieda
K
K
D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D004277:DNA, Single-Stranded