A potential splicing factor is encoded by the opposite strand of the trans-spliced c-myb exon.
We previously established that the expression of a thymic c-myb mRNA species requires the intermolecular recombination of coding sequences expressed from transcriptional units localized on different chromosomes, in both chicken and human. We now report that a putative splicing factor (PR264), extremely well conserved in chicken and human, is encoded by the opposite strand of the c-myb trans-spliced exon. The PR264 polypeptide, which contains a typical ribonucleoprotein 80 and an arginine/serine-rich domain, is highly homologous to the Drosophila splicing regulators tra, tra-2, and su(wa) and to the human alternative splicing factor ASF/SF2. Furthermore, we show that PR264-specific mRNAs are expressed in normal hematopoietic cells of chicken and human origin and that the relative proportion of the PR264 transcripts is developmentally regulated in chicken.
Vellard
M
M
Laboratoire d'Oncologie Virale et Moléculaire, Institut Curie, Centre Universitaire, Orsay, France.
Sureau
A
A
Soret
J
J
Martinerie
C
C
Perbal
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012326:RNA Splicing; D016372:RNA, Antisense / Q000235:genetics; D012261:Ribonucleoproteins / Q000235:genetics*; D016415:Sequence Alignment; D000068103:Serine-Arginine Splicing Factors; D014158:Transcription, Genetic
D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D016372:RNA, Antisense; D012261:Ribonucleoproteins; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors; D004247:DNA