Induction of cytotoxicity by photoexcited TiO2 particles.
Photoexcited TiO2 particles can drive various chemical reactions due to their strong oxidizing and reducing ability. To investigate the possible use of this effect for cancer treatment, the antitumor activity of photoexcited TiO2 particles was studied in vitro and in vivo. HeLa cells cultured in vitro were completely killed in the presence of TiO2 (50 micrograms/ml) with 10-min UV irradiation by a 500-W-Hg lamp. In contrast, very little cell death was observed from TiO2 treatment without UV irradiation. Photoexcited TiO2 particles also significantly suppressed the growth of HeLa cells implanted in nude mice, compared with those receiving TiO2 alone or UV irradiation alone. The cell death caused by photoexcited TiO2 particles was significantly protected in the presence of L-tryptophan and catalase. These molecules are quenchers of hydroxyl radicals and scavengers of hydrogen peroxide, respectively, suggesting that the cells were killed by the OH. and H2O2 produced from photoexcited TiO2 particles.
Cai
R
R
Department of Synthetic Chemistry, University of Tokyo, Hongo, Japan.
Kubota
Y
Y
Shuin
T
T
Sakai
H
H
Hashimoto
K
K
Fujishima
A
A
D000818:Animals; D002470:Cell Survival; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells / Q000528:radiation effects*; D006801:Humans; D006859:Hydrogen / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D014025:Titanium / Q000494:pharmacology*; D014467:Ultraviolet Therapy
C009495:titanium dioxide; D006859:Hydrogen; D006861:Hydrogen Peroxide; D014025:Titanium