SIV/HIV recombinants and their use in studying biological properties.
A series of chimeric clones between human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency viruses (SIV) were constructed. Viability of the recombinant viruses was dependent on the position of recombination. Infectious chimeric viruses between HIV-1 and SIVAGM (isolated from an African green monkey) and those between HIV-1 and SIVMAC (isolated from a rhesus monkey) were examined for host cell tropism. Viral determinants that restrict the replication of SIVAGM in human MT-4 cells and that of HIV-1 in macaque monkey peripheral blood mononuclear cells (PBMC) mapped to the 5' half of the virus genome. One HIV-1/SIVMAC chimera which contained the HIV-1 env gene was shown to replicate in macaque PBMC in vitro and to infect macaque monkeys in vivo. This HIV-1/SIVMAC chimera will be useful for a variety of AIDS pathogenesis and vaccine studies.
Shibata
R
R
Institute for Virus Research, Kyoto University, Japan.
Adachi
A
A
D000818:Animals; D003001:Cloning, Molecular; D005814:Genes, Viral / Q000235:genetics; D015658:HIV Infections / Q000382:microbiology; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D016865:Reassortant Viruses / Q000502:physiology*; D011995:Recombination, Genetic; D016097:Simian Acquired Immunodeficiency Syndrome / Q000382:microbiology; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology; D014779:Virus Replication / Q000235:genetics / Q000502:physiology
D014764:Viral Proteins