Characterization of a high affinity binding site for pancreatic-type phospholipase A2 in the rat. Its cellular and tissue distribution.
We showed in an earlier study (Arita, H., Hanasaki, K., Nakano, T., Oka, S., Teraoka, H., and Matsumoto, K. (1991) J. Biol. Chem. 266, 19139-19141) that there is a high affinity and specific binding site for mammalian group I phospholipase A2 (PLA2-I) in Swiss 3T3 fibroblast cells. Analysis of the cellular distribution in rat using 125I-PLA2-I as a radioligand indicated the presence of this site in various cells, including vascular smooth muscle cells (VSMC), vascular endothelial cells, synovial cells, chondrocytes, and gastric mucosal cells. Scatchard analysis of rat VSMC revealed the existence of a single class of binding site with a Kd value of 1.60 nM and a Bmax value of 51.0 fmol/10(6) cells. The mammalian mature type of PLA2s-I derived from several animal species specifically recognized the same site in cells and stimulated DNA synthesis, whereas its inactive zymogen, mammalian group II PLA2s, and snake and bee venom PLA2s showed much lesser activities. 125I-PLA2-I bound to VSMC was rapidly internalized and subsequently released from the cells as trichloroacetic acid-soluble radioactivity. Down-regulation of the PLA2-I site was observed in the treatment of VSMC with cAMP-elevating agents, as well as glucocorticoids. Affinity labeling experiments indicated that PLA2-I binds to a single polypeptide with a mass of approximately 200,000 daltons. These results suggest a novel PLA2-I action on the cellular function via its specific binding site.
Hanasaki
K
K
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Arita
H
H
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D009928:Organ Specificity; D010179:Pancreas / Q000201:enzymology*; D010741:Phospholipases A / Q000378:metabolism* / Q000494:pharmacology; D054467:Phospholipases A2; D051381:Rats; D013045:Species Specificity; D013552:Swine; D013583:Synovial Membrane / Q000201:enzymology
D007527:Isoenzymes; D010741:Phospholipases A; D054467:Phospholipases A2