title: Detection of subtle reciprocal translocations by fluorescence in situ hybridization.
abstract: Three different subtle reciprocal translocations were detected on long, well-banded chromosomes. The same translocations were examined using fluorescence in situ hybridization (FISH) with chromosome-specific libraries and unique DNA sequences. Our findings show that FISH allows rapid and unequivocal detection and characterization of this type of chromosome rearrangement. This approach is especially useful for prenatal diagnosis when one of the parents is a balanced carrier of such small fragment translocations.
authors.affiliation: Department of Medical Genetics, University Hospital, Ghent, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Speleman
authors.forename: N
authors.initials: N
authors.lastname: Van Roy
authors.forename: J
authors.initials: J
authors.lastname: Wiegant
authors.forename: M R
authors.initials: MR
authors.lastname: Verschraegen-Spae
authors.forename: Y
authors.initials: Y
authors.lastname: Benoit
authors.forename: P
authors.initials: P
authors.lastname: Govaert
authors.forename: L
authors.initials: L
authors.lastname: Goossens
authors.forename: J G
authors.initials: JG
authors.lastname: Leroy
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005333:Fetus / Q000002:abnormalities; D005456:Fluorescent Dyes; D006801:Humans; D007223:Infant; D008297:Male; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods; D014178:Translocation, Genetic / Q000235:genetics*
chemical_list: D005456:Fluorescent Dyes