Epidermal growth factor binding sites on human erythrocytes in donors with different ABO blood groups.
Analysis of epidermal growth factor (125J-EGF) binding to human red cells revealed the presence of two classes of binding sites with apparent equilibrium dissociation constants (app.Kd) in the 10(-10)-10(-9) M and in the 10(-8) M range, respectively. The number of binding sites/cell ranged between 600 and 2,400 for the high-affinity binding site and between 7,200 and 23,000 for the low-affinity site. No differences were seen in the apparent Kd values for both types of binding sites between red cells obtained from donors with different ABO-blood groups. An increase in the number of high affinity EGF binding sites was observed in donors with blood group A1-erythrocytes as compared to red cells taken from donors with blood groups O and B.
Engelmann
B
B
Physiologisches Institut, Universität München, West Germany.
Schumacher
U
U
Haen
E
E
D000017:ABO Blood-Group System / Q000235:genetics / Q000276:immunology*; D001665:Binding Sites; D001782:Blood Donors; D004815:Epidermal Growth Factor / Q000276:immunology / Q000378:metabolism*; D066246:ErbB Receptors / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D014644:Genetic Variation / Q000276:immunology; D006648:Histocompatibility / Q000276:immunology; D006801:Humans; D007519:Isoantigens / Q000502:physiology; D011485:Protein Binding / Q000276:immunology; D012044:Regression Analysis
D000017:ABO Blood-Group System; D007519:Isoantigens; D004815:Epidermal Growth Factor; D066246:ErbB Receptors