title: [Effects of experimental Listeria monocytogenes infection on mice fed on lamprey or sardine oil prepared under high-temperature deodorization].
abstract: Fresh lamprey (F-La) or sardine (F-Sa) oil is known to contain a large amount of n-3 polyunsaturated fatty acids such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. When F-La or F-Sa was deodorized with steam at 280 degrees C under 1 mmHg for 1 h (H-La and H-Sa, respectively), the contents of EPA and DHA were reduced and unidentified peaks were newly detected by gas-liquid chromatography. To know the biological influences of these high-temperature deodorized oils, the sterilizing function of the macrophage against Listeria monocytogenes was investigated in male ddY mice fed H-La or H-Sa. One week feeding of H-La or H-Sa lowered the LD50 values of the bacteria injected intravenously. Numbers of the viable bacteria on the day 3 after intravenous injection were about 10 times higher in the liver and 5 times higher in the spleen of mice fed H-La or H-Sa as compared with those of the control group. These results suggest that the sterilizing function of fixed macrophages both in the liver and the spleen was suppressed in mice fed H-La or H-Sa.
authors.affiliation: Department of Pharmacology, Kohno Clinical Medicine Research Institute, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Mineo
authors.forename: Y
authors.initials: Y
authors.lastname: Konishi
authors.forename: K
authors.initials: K
authors.lastname: Satoh
authors.forename: A
authors.initials: A
authors.lastname: Yanagisawa
authors.forename: K
authors.initials: K
authors.lastname: Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Kurata
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D005395:Fish Oils / Q000494:pharmacology*; D006358:Hot Temperature; D007928:Lethal Dose 50; D008087:Listeria / Q000472:pathogenicity; D008088:Listeriosis / Q000382:microbiology*; D008099:Liver / Q000382:microbiology; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D009812:Odorants; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects*; D013154:Spleen / Q000382:microbiology
chemical_list: D005395:Fish Oils