Magnificent expression of asialo GM1 on thymus cells and spleen T cells of the musk shrew, Suncus murinus.
The expression of asialo GM1 (GA1) was observed on almost all thymocytes from young musk shrew, at the age of 4 weeks by flow cytometric analysis. In adult shrew aged 10 months, the ratio of GA1-negative thymocytes was increased. Among several anti-glycolipid antibodies used, anti-GM1 and anti-Forssman also reacted with the thymocytes. Protein fraction of the thymocytes was analyzed by SDS-PAGE followed by immunoblotting. Anti-GA1 and anti-GM1 showed two bands and one band, respectively, however, their mobilities were different from each other. Anti-Forssman did not stain any protein. The GA1-positive population in spleen T cell fraction was not detected in young shrew but most of the T cells were changed to GA1-positive cells in adult shrew. When mixed lymphocyte culture was performed, the GA1-negative spleen T cells in young shrew were changed to express GA1 marker on their cell surface by differentiation. Abbreviations used were as follows: GA1, Gal beta 1-3GalNAc beta 1-4Gal beta 1-4Glc-Cer; GM1, Gal beta 1-3GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-4Glc-Cer; Forssman, GalNAc alpha 1-3GalNAc beta 1-3Gal alpha 1-4Gal beta 1-4Glc-Cer.
Esaki
E
E
Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan.
Okada
M
M
Fujii
Y
Y
Nagai
Y
Y
Kitoh
J
J
Kato
N
N
Naiki
M
M
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D005434:Flow Cytometry; D005677:G(M1) Ganglioside / Q000032:analysis*; D015151:Immunoblotting; D012788:Shrews / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000737:chemistry* / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology
D000954:Antigens, Surface; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside