Androgen receptor expression and 5 alpha-reductase activity along the proximal-distal axis of the rat prostatic duct.
The rat prostate consists of a series of branched ducts that eminate from the urethra. Heterogeneity of rat prostatic growth, secretory activity, and cell turnover has been observed along the proximal-distal axis of the branched ductal network. In addition, there are regional differences in androgen sensitivity along the ducts, with the distal ductal tips being highly androgen dependent and the proximal regions being relatively androgen independent. To determine the underlying mechanisms that may regulate these regional differences in androgen responsiveness, androgen receptor (AR) levels and 5 alpha-reductase activity were examined along the proximal-distal axis of microdissected ventral prostatic ducts from 15-, 30-, and 100-day-old rats. As in the murine prostate, DNA synthetic activity was concentrated in the distal tip region of the 15- and 30-day ducts. Immunocytochemistry and autoradiography with [3H] dihydrotestosterone were used to examine AR expression and functional ability to bind ligand, respectively. The results revealed no discernable differences in AR levels or binding activity in any cell type along the ductal length in prepubertal, pubertal, or adult rats. In addition, 5 alpha-reductase activity was the same in the distal and proximal ductal regions. We conclude that regional heterogeneity in prostatic growth and function is not a result of differences in levels of AR and 5 alpha-reductase. Rather, other region-specific structural, intracellular, or paracrine factors may be responsible for the differences in androgen responsiveness along the prostatic duct.
Prins
G S
GS
Department of Obstetrics and Gynecology, Humana-Michael Reese Hospital, University of Illinois College of Medicine, Chicago 60616.
Cooke
P S
PS
Birch
L
L
Donjacour
A A
AA
Yalcinkaya
T M
TM
Siiteri
P K
PK
Cunha
G R
GR
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism*; D000375:Aging; D000818:Animals; D001345:Autoradiography; D004261:DNA Replication; D013196:Dihydrotestosterone / Q000378:metabolism; D007150:Immunohistochemistry; D007700:Kinetics; D008297:Male; D011467:Prostate / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011944:Receptors, Androgen / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D014316:Tritium; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; D013936:Thymidine