title: Molecular cloning and characterization of type VII collagen cDNA.
abstract: Type VII collagen, located in human epidermal basement membrane, is the primary pathogenic target molecule in epidermolysis bullosa acquisita and epidermolysis bullosa dystrophica. Using a monoclonal antibody against the non-collagenous domain of type VII collagen, approximately 1 Kb cDNA was isolated from human keratinocyte library. The deduced primary structure of this clone thus reflects the non-collagenous domain of type VII collagen that may be involved in cell attachment. This region shows a weak homology (approximately 23%) to the cell attachment domain of fibronectin. Northern blot revealed approximately 9.5 Kb single band.
authors.affiliation: Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: F
authors.initials: F
authors.lastname: Furukawa
authors.forename: S
authors.initials: S
authors.lastname: Imamura
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003094:Collagen / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics; D015723:Gene Library; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000911:Antibodies, Monoclonal; D004277:DNA, Single-Stranded; D003094:Collagen