title: Chorionic villus metaphase chromosomes and interphase nuclei analysed by chromosomal in situ suppression (CISS) hybridization.
abstract: Metaphase chromosomes and interphase nuclei of chorionic villus samples (CVS) in five cases were studied after treatment with trypsin and post-fixation in formaldehyde by chromosomal in situ suppression (CISS) hybridization. Our modified protocol enables the use of in situ hybridization techniques on CVS preparations after 42 h of culture. A balanced translocation and trisomy 13 were identified with the aid of CISS hybridization.
authors.affiliation: Institut für Humangenetik und Anthropologie, Heidelberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Klever
authors.forename: C J
authors.initials: CJ
authors.lastname: Grond-Ginsbach
authors.forename: H D
authors.initials: HD
authors.lastname: Hager
authors.forename: T M
authors.initials: TM
authors.lastname: Schroeder-Kurth
mesh_terms: D015193:Chorionic Villi Sampling / Q000379:methods*; D002869:Chromosome Aberrations / Q000175:diagnosis*; D025063:Chromosome Disorders; D002882:Chromosomes, Human, Pair 13; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014178:Translocation, Genetic; D014314:Trisomy