Tropoelastin gene expression in the rat pulmonary vasculature: a developmental study.
The elastic laminae in a vessel provide resilience to its wall. In perinatal and adult rats, we used in situ hybridization to localize the mRNA for tropoelastin (TE) in endothelial cells, medial smooth muscle cells, and adventitial fibroblasts of pulmonary arteries and veins to determine the contribution of these cells to laminae formation. We found that 1) all three cell types are elastogenic but for each the ontogenic pattern is different, 2) signal in the artery is strongest in the late fetal lung, 3) postnatally TE expression decreases first in the outer medial smooth muscle cells, and 4) the pattern of expression in arteries differs from that in veins. In the d 19 fetus, the signal for TE mRNA was higher in arteries than in veins. In the immediate postnatal period, the arterial signal declined, whereas the signal in veins increased. By postnatal d 21, the arterial TE signal per cell had significantly decreased to an intensity lower than that in veins. In the adult rat lung, no TE mRNA was detected by in situ hybridization. The reciprocal alterations in TE expression in pulmonary arteries and veins may suggest a response to the postnatal change in pulmonary blood pressure. We speculate that because all three cell types are potentially elastogenic they may all play a role in the remodeling that occurs after vascular injury.
Noguchi
A
A
Department of Pediatrics, Cardinal Glennon Children's Hospital, St. Louis University, School of Medicine, Missouri 63104.
Samaha
H
H
deMello
D E
DE
D000818:Animals; D015342:DNA Probes; D004547:Elastic Tissue / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D004730:Endothelium, Vascular / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D024510:Muscle Development; D009131:Muscle, Smooth, Vascular / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D011247:Pregnancy; D011651:Pulmonary Artery / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D011667:Pulmonary Veins / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014333:Tropoelastin / Q000235:genetics*
D015342:DNA Probes; D012333:RNA, Messenger; D014333:Tropoelastin