Protection against cartilage proteoglycan synthesis inhibition by antiinterleukin 1 antibodies in experimental arthritis.
We have used neutralizing antibodies raised against murine recombinant interleukin 1 (IL-1) to demonstrate a role for IL-1 in the cartilage destruction and inflammation of antigen induced arthritis. Ex vivo production of IL-1 was demonstrated in tissue cultures of joint cross sections shortly after arthritis induction. Neutralizing antimurine IL-1 antibodies identified the activity to be about 80% IL-1 alpha 24 h after onset of arthritis. In animals receiving a single injection of anti-IL-1 antisera at Day -3, cartilage proteoglycan synthesis suppression during the first 2 days of arthritis was prevented. Normal proteoglycan synthesis was maintained until Day 4 when anti-IL-1 antisera was given at Days -2, 0, and 2 or arthritis. Dose response experiments showed that the reduction in inflammation was insufficient to account for the clearcut reduction in cartilage proteoglycan synthesis inhibition. Our results demonstrate that IL-1 plays a role in cartilage pathology in murine antigen induced arthritis.
van de Loo
F A
FA
Department of Rheumatology, University Hospital St. Radboud, Nijmegen, The Netherlands.
Arntz
O J
OJ
Otterness
I G
IG
van den Berg
W B
WB
D000818:Animals; D000906:Antibodies / Q000502:physiology* / Q000627:therapeutic use; D001168:Arthritis / Q000276:immunology / Q000378:metabolism* / Q000628:therapy; D002358:Cartilage, Articular / Q000378:metabolism*; D007167:Immunotherapy; D007375:Interleukin-1 / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D011509:Proteoglycans / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D012710:Serum Albumin, Bovine / Q000276:immunology; D013583:Synovial Membrane / Q000378:metabolism
D000906:Antibodies; D007375:Interleukin-1; D011509:Proteoglycans; D012710:Serum Albumin, Bovine