Action of Escherichia coli heat-stable enterotoxin II on isolated sections of mouse ileum.
When Escherichia coli STII was applied to the serosa of the ileum at a concentration of 40 micrograms/ml, an acceleration of the spontaneous motility and a weak contraction were induced 2-3 min later. The induction was not affected by the addition of atropine (10(-6) M), but was abolished by the addition of papaverine (10(-4) M). When STII was applied to the mucosa, the acceleration of the spontaneous motility appeared 7-8 min later, but a contraction was not induced. These results suggest that STII acts directly on muscle cell of the ileum and enhances the spontaneous motility of the intestine.
Hitotsubashi
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
Akagi
M
M
Saitou
A
A
Yamanaka
H
H
Fujii
Y
Y
Okamoto
K
K
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001427:Bacterial Toxins / Q000633:toxicity*; D003470:Culture Media; D004768:Enterotoxins / Q000633:toxicity*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005769:Gastrointestinal Motility / Q000187:drug effects; D006358:Hot Temperature; D007082:Ileum / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D010208:Papaverine / Q000494:pharmacology
D001427:Bacterial Toxins; D003470:Culture Media; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli); D001285:Atropine; D010208:Papaverine