Enhanced clearance of lactic dehydrogenase-5 in severe combined immunodeficiency (SCID) mice: effect of lactic dehydrogenase virus on enzyme clearance.
The lactic dehydrogenase (LDH) level in plasma and the clearance of LDH in C.B-17 scid (severe combined immunodeficiency; SCID) mice were compared with those in C.B-17 or BALB/cCrSlc mice with or without lactic dehydrogenase virus (LDV) infection. The resting enzyme level in SCID mice showed little difference from that in C.B-17 or BALB/cCrSlc mice. The degree of increased plasma LDH level in SCID mice was lower than that in C.B-17 and BALB/cCrSlc mice after LDV infection. To assess the mechanisms of decrease in LDH elevation in SCID mice infected with LDV, virus replication was compared in SCID and BALB/cCrSlc mice. The infectivity titre of plasma in SCID mice was higher (more than 10 times) than that in BALB/cCrSlc mice. Moreover, the percentage of virus antigen positive Kupffer cells was higher in SCID mice than that in BALB/cCrSlc mice. The level of endogenous LDH release as a result of carbon tetrachloride treatment was similar in the SCID and BALB/cCrSlc mice. The clearance rate of endogenous LDH was greater in SCID mice than in BALB/cCrSlc mice with or without LDV infection. The rate of clearance of intravenously injected porcine LDH-5, but not porcine LDH-1, was enhanced in SCID mice as compared with that in BALB/cCrSlc mice. Furthermore, carbon clearance was higher in SCID mice than that in BALB/cCrSlc mice. These results suggest that the smaller increase of plasma LDH after infection might be due, at least in part, to the enhanced LDH-5 clearance function by macrophages in SCID mice.
Hayashi
T
T
Laboratory of Veterinary Pathology, University of Yamaguchi, Japan.
Ozaki
M
M
Mori
I
I
Saito
M
M
Itoh
T
T
Yamamoto
H
H
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002251:Carbon Tetrachloride / Q000494:pharmacology; D007153:Immunologic Deficiency Syndromes / Q000201:enzymology*; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D007772:Lactate dehydrogenase-elevating virus / Q000276:immunology / Q000302:isolation & purification*; D008264:Macrophages / Q000201:enzymology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID / Q000097:blood*; D013997:Time Factors; D014777:Virus Diseases / Q000201:enzymology*
D000956:Antigens, Viral; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase