title: Heterogeneous induction of 72-kDa heat shock protein (HSP72) in cultured mouse oligodendrocytes and astrocytes.
abstract: The expression of 72-kDa heat shock protein (HSP72) in cultured mouse oligodendrocytes and astrocytes exposed to heat shock was investigated by double immunolabelling with anti-HSP72 monoclonal antibody (C92F3B-1) and antibodies against galactocerebroside (GalC) or glial fibrillary acidic protein (GFAP). After 3 h recovery from heat shock, an intermediate level of HSP72 immunolabelling was localized in the nucleolus and cytoplasm of astrocytes (less than 25%) and to a lesser extent in oligodendrocytes (less than 2%). After 8-48 h, HSP72 was expressed intensely in the cytoplasm and nuclear matrix of oligodendrocytes (20-40%), while weak/intermediate immunostaining was detectable in astrocytes (5-15%). The levels of HSP72 expressed in oligodendrocytes and astrocytes decreased around 72-120 h, but a few oligodendrocytes (4%) remained intensely immunolabelled. These results indicate that heat shock induces HSP72 in both oligodendrocytes and astrocytes. However, this response is heterogeneous.
authors.affiliation: Division of Demyelinating Diseases and Aging, National Institute of Neuroscience, NCNP, Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Satoh
authors.forename: T
authors.initials: T
authors.lastname: Yamamura
authors.forename: T
authors.initials: T
authors.lastname: Kunishita
authors.forename: T
authors.initials: T
authors.lastname: Tabira
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000378:metabolism* / Q000648:ultrastructure; D015153:Blotting, Western; D002478:Cells, Cultured; D005260:Female; D005699:Galactosylceramides / Q000096:biosynthesis / Q000276:immunology; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000276:immunology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009836:Oligodendroglia / Q000378:metabolism* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D005699:Galactosylceramides; D005904:Glial Fibrillary Acidic Protein; D006360:Heat-Shock Proteins