Influence of ethanol on survival of acinar cells isolated from rat pancreas.
The role of ethanol in precipitating acute pancreatitis has been studied in model experiments. The influence of ethanol on the survival of isolated pancreatic acinar cells (PAC) and a possible coaction with noxious agents (such as trypsin, chymotrypsin, temporary anoxia, and partial uncoupling of the oxidative phosphorylation by 2,4-dinitrophenol [DNP]) has been investigated. Isolated PACs withstood ethanol levels up to 180 mM without significant decrease of viability within 4 h incubation at 37 degrees C. A 90-min deprivation of oxygen was widely tolerated also. However, with a combination of both ethanol application and oxygen deprivation, a clearly forced cell damage was observed as was true with a combination of ethanol and chymotrypsin. The action of DNP or of extracellular trypsin on cell survival was not amplified by the addition of ethanol (180 mM). This study reveals two possible sites of action at which ethanol may contribute to the pathogenesis of acute pancreatitis.
Siech
M
M
Division of Experimental Surgery, Medical Academy of Magdeburg, Federal Republic of Germany.
Letko
G
G
D019297:2,4-Dinitrophenol; D000208:Acute Disease; D000818:Animals; D015687:Cell Hypoxia / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002918:Chymotrypsin / Q000633:toxicity; D004140:Dinitrophenols / Q000633:toxicity; D004347:Drug Interactions; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D066298:In Vitro Techniques; D010179:Pancreas / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010195:Pancreatitis / Q000209:etiology; D051381:Rats; D014357:Trypsin / Q000633:toxicity
D004140:Dinitrophenols; D000431:Ethanol; D002918:Chymotrypsin; D014357:Trypsin; D019297:2,4-Dinitrophenol