Studies of an improved rhesus hematopoietic progenitor cell assay.
We have improved Rhesus monkey marrow cell growth in semisolid media by means of substituting supplemented calf serum for fetal bovine serum. The cloning efficiency of light-density marrow cells separated on 60% Percoll was 126 (+/- 54)/10(5) (n = 12, +/- SD), and for light-density peripheral blood cells 60 (+/- 46)/10(6) (n = 11). Thirty-five percent of the colonies were multilineage, whereas the remainder were unilineage colonies composed of erythrocytes, megakaryocytes, and neutrophilic or monocytic granulocytes. Unilineage megakaryocyte colonies comprised 12% of the total marrow progenitor cells. The [3H]TdR suicide index of marrow progenitor cells was 47% +/- 9% (n = 12). This progenitor cell assay should prove useful in preclinical studies of the effect of recombinant hematopoietic growth factors on the number and cycling status of Rhesus hematopoietic progenitor cells.
Winton
E F
EF
Emory University, Department of Medicine, Atlanta, Georgia.
Jacobs
P C
PC
Rozmiarek
S K
SK
Stahl
C P
CP
Myers
L A
LA
Liehl
E
E
Stoll
R E
RE
Anderson
D C
DC
McClure
H M
HM
D000818:Animals; D001854:Bone Marrow Cells; D002999:Clone Cells; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D066298:In Vitro Techniques; D008253:Macaca mulatta / Q000097:blood*; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism
D010980:Platelet Membrane Glycoproteins