[Distribution of 5-, 12-lipoxygenases in porcine ciliary epithelial cells].
Nonpigmented epithelial cells (NPE) and pigmented epithelial cells (PE) were separated from porcine ciliary processes without protease treatment. The relative capabilities of NPE and PE to synthesize 5-hydroxyeicosatetraenoic acid (5-HETE), 12-HETE, 5,12-diHETE from 1- (14C) arachidonic acid were determined by high pressure liquid chromatography. The capability of NPE to synthesize 5-lipoxygenase and 12-lipoxygenase products was, respectively, 5-fold and 2,5-fold higher than that of PE. The formation of 5-HETE, 12-HETE and 5,12-diHETE was inhibited almost completely by 50 microM nordihydroguaiaretic acid. It was suggested that NPE played a role in the regulation of aqueous humor formation and intraocular inflammation.
Sakamoto
S
S
Department of Ophthalmology Osaka University Medical School, Japan.
D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism*; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D002924:Ciliary Body / Q000201:enzymology*; D004848:Epithelium / Q000201:enzymology; D010857:Pigment Epithelium of Eye / Q000201:enzymology; D013552:Swine
D001092:Arachidonate 12-Lipoxygenase; D001094:Arachidonate 5-Lipoxygenase