title: Human fibroblast cells synthesize and secrete nerve growth factor in culture.
abstract: Using our enzyme immunoassay system developed for recombinant hNGF, we examined the synthesis and secretion of human NGF (hNGF) by human fibroblast (WS-1) cells. The amount of the factor secreted by WS-1 cells increased linearly and a significant amount of NGF was detected in the conditioned medium of WS-1 cultures. WS-1 NGF showed properties identical to those of recombinant human NGF in immunoreactivity and molecular weight. An increase in cell density or the withdrawal of serum from the culture medium caused a drastic decrease in the rate of NGF secretion. These results suggest that WS-1 cells are able to synthesize and secrete hNGF in culture and that the synthesis/secretion is regulated in a growth phase-dependent manner.
authors.affiliation: Department of Molecular Biology, Gifu Pharmaceutical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Murase
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Takayanagi
authors.forename: Y
authors.initials: Y
authors.lastname: Furukawa
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D002455:Cell Division; D002460:Cell Line; D002850:Chromatography, Gel; D005347:Fibroblasts; D006801:Humans; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis
chemical_list: D009414:Nerve Growth Factors; D011994:Recombinant Proteins