Ribonucleoprotein components of root meristematic cell nuclei of the tomato characterized by application of mild loosening and immunocytochemistry.
Immunocytochemistry and hypotonic-formaldehyde fixation have been used to study the extranucleolar ribonucleoprotein (RNP) constituents of the nucleus of tomato root meristematic cells. The study of the distribution of small nuclear uridine-rich RNPs (snRNP) by means of a monoclonal anti-Sm antibody recognizing a 29-kDa protein in plants, after standard fixation, shows a preferential labeling of the perichromatin region and a lower labeling of the interchromatin space. These results suggest that in the tomato there is a perichromatin region similar to that of animal cells, in which much of the nonnucleolar transcription and splicing takes place. In hypotonic-formaldehyde-detergent-fixed nuclei, fibrogranular polyparticles have been visualized reacting with anti-snRNP antibody. These structures are frequently associated with filaments of extended chromatin characterized by their reaction with an anti-DNA monoclonal antibody.
Vázquez-Nin
G H
GH
Laboratory of Electron Microscopy, Faculty of Sciences, National Autonomous University of México, D.F.
Echeverría
O M
OM
Mínguez
A
A
Moreno Díaz de la Espina
S
S
Fakan
S
S
Martin
T E
TE
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000737:chemistry*; D005404:Fixatives; D007150:Immunohistochemistry; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000737:chemistry; D009994:Osmolar Concentration; D010940:Plant Proteins / Q000737:chemistry; D010944:Plants / Q000737:chemistry / Q000648:ultrastructure*; D012261:Ribonucleoproteins / Q000737:chemistry*
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D005404:Fixatives; D009687:Nuclear Proteins; D010940:Plant Proteins; D012261:Ribonucleoproteins