title: A site-directed mutagenesis study on the role of isoleucine-23 of human epidermal growth factor in the receptor binding.
abstract: The isoleucine-23 residue of human epidermal growth factor (hEGF) was substituted by a variety of amino acid residues and the receptor-binding activities of variant hEGFs were determined by the use of human KB cell. Tight receptor binding was found of variants with hydrophobic amino acid residues in position 23. The size of the isoleucine residue was nearly optimum for the receptor binding as compared with other hydrophobic residues. The structure analysis by two-dimensional nuclear magnetic resonance spectroscopy showed that the substitution at position 23 only slightly affected the tertiary structure of hEGF. These indicate that the side chain of isoleucine residue in position 23, which is exposed on the protein surface, directly binds to a hydrophobic pocket of the receptor.
authors.affiliation: Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Koide
authors.forename: Y
authors.initials: Y
authors.lastname: Muto
authors.forename: H
authors.initials: H
authors.lastname: Kasai
authors.forename: K
authors.initials: K
authors.lastname: Kohri
authors.forename: K
authors.initials: K
authors.lastname: Hoshi
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Tsukumo
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: T
authors.initials: T
authors.lastname: Miyake
mesh_terms: D000595:Amino Acid Sequence; D001667:Binding, Competitive; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D066246:ErbB Receptors / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D006801:Humans; D007532:Isoleucine / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D012995:Solubility
chemical_list: D007532:Isoleucine; D004815:Epidermal Growth Factor; D066246:ErbB Receptors