Cloning a replication function from the streptomycete bacteriophage FP43.
Streptomyces griseofuscus cells carrying a 4.4-kb SphI DNA fragment from bacteriophage FP43 inhibited plaque formation (Pin) by FP43, and the Pin function was localized to a 0.96-kb SacII fragment. The same 4.4-kb SphI fragment was able to replicate freely in several streptomycetes, including S. griseofuscus, and the replication (Rep) function was localized to a 1.2-kb SphI-FspI fragment. Plasmids with FP43 Rep function are unstable and are present at about 20-50 copies per chromosome. Plasmids with FP43 Rep function are compatible with SCP2* plasmids.
Howell
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Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285-0424.
Rao
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D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D004261:DNA Replication / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*; D010948:Viral Plaque Assay
D004279:DNA, Viral