TEM cytochemical study of the localization of phospholipids in interphase chromatin in rat hepatocytes.
The electron microscopy cytochemical detection of phospholipids in well-defined areas in the interphase nuclei of hepatocytes has been obtained by the acid haematein test, modified for electron microscopy and by the phospholipase A2-colloidal gold method. The specificity of both methods were controlled by enzymatic digestion with phospholipase. The main intra-nuclear localization of phospholipids is at the border between the condensed and dispersed chromatin, where non-ribosomal RNA is also revealed by RNase-gold labelling. Phospholipids are detected, too, over the clusters of interchromatin granules and in the fibrillar component of the nucleolus.
Fraschini
A
A
Dipartimento di Biologia Animale dell'Università di Pavia, Italy.
Albi
E
E
Gahan
P B
PB
Viola-Magni
M P
MP
D000818:Animals; D002843:Chromatin / Q000737:chemistry*; D005260:Female; D007150:Immunohistochemistry / Q000379:methods*; D007399:Interphase; D008099:Liver / Q000737:chemistry / Q000166:cytology; D008297:Male; D008854:Microscopy, Electron; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D002843:Chromatin; D010743:Phospholipids