title: The B cell-specific nuclear factor OTF-2 positively regulates transcription of the human class II transplantation gene, DRA.
abstract: The promoter of the major histocompatibility class II gene DRA contains an octamer element (ATTTGCAT) that is required for efficient DRA expression in B cells. Several DNA-binding proteins are known to bind this sequence. The best characterized are the B cell-specific OTF-2 and the ubiquitous OTF-1. This report directly demonstrates that OTF-2 but not OTF-1 regulates the DRA gene. In vitro transcription analysis using protein fractions enriched for the octamer-binding protein OTF-2 demonstrate a positive functional role for OTF-2 in DRA gene transcription. In contrast, OTF-1-enriched protein fractions did not affect DRA gene transcription although it functionally enhanced the transcription of another gene. Recombinant OTF-2 protein produced by in vitro transcription/translation could also enhance DRA gene transcription in vitro. In vivo transient transfection studies utilizing an OTF-2 expression vector resulted in similar findings: that OTF-2 protein enhanced DRA gene transcription, and that this effect requires an intact octamer element. Together these results constitute the first direct evidence of a positive role for the lymphoid-specific octamer-binding factor in DRA gene transcription.
authors.affiliation: Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599-7295.
authors.forename: N J
authors.initials: NJ
authors.lastname: Zeleznik-Le
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh-Lindstrom
authors.forename: J B
authors.initials: JB
authors.lastname: Clarke
authors.forename: T L
authors.initials: TL
authors.lastname: Moore
authors.forename: J P
authors.initials: JP
authors.lastname: Ting
mesh_terms: D001345:Autoradiography; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D002855:Chromatography, Thin Layer; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D050813:Octamer Transcription Factor-2; D010957:Plasmids; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; D050813:Octamer Transcription Factor-2; C058435:POU2F2 protein, human; D011994:Recombinant Proteins; D014157:Transcription Factors; D004247:DNA