title: Decreased interleukin 1 activity released from circulating monocytes of patients with familial Mediterranean fever during in vitro stimulation by lipopolysaccharide.
abstract: Familial Mediterranean fever (FMF) is an inherited disorder of unknown etiology characterized by recurrent episodes of serous membrane inflammation. Interleukin 1 (IL-1) is a mediator of inflammatory processes. We hypothesized that IL-1 may play a role in acute attacks of FMF. Thus we tested IL-1 production by monocytes derived from patients with FMF. Nine patients were tested during acute attacks and 9 were asymptomatic when tested. Monocytes derived from peripheral blood of patients and controls were stimulated with lipopolysaccharide (LPS) and IL-1 activity in the supernatant was tested using a T helper cell line (D10-4G.1). IL-1 secretion during acute attacks was decreased whereas IL-1 production in asymptomatic patients was comparable to healthy controls. Followup of symptomatic patients during the recovery period revealed normalization of IL-1 secretion. Addition of indomethacin (prostaglandin E2 inhibitor) to LPS stimulated monocytes did not change IL-1 activity in patients or healthy controls. We conclude that in vitro IL-1 activity in patients with FMF is associated with the intensity of the inflammatory process.
authors.affiliation: Department of Rheumatology, Bnai Zion Medical Center, Haifa, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Rozenbaum
authors.forename: R
authors.initials: R
authors.lastname: Katz
authors.forename: I
authors.initials: I
authors.lastname: Rozner
authors.forename: S
authors.initials: S
authors.lastname: Pollack
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D010505:Familial Mediterranean Fever / Q000097:blood*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000097:blood*; D008070:Lipopolysaccharides; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D012016:Reference Values; D013268:Stimulation, Chemical
chemical_list: D016861:Cyclooxygenase Inhibitors; D007375:Interleukin-1; D008070:Lipopolysaccharides