title: A role for proteoglycans in the guidance of a subset of pioneer axons in cultured embryos of the cockroach.
abstract: Several molecules involved in the development of the nervous system have specific binding sites for the glycosaminoglycan (GAG) side chains of proteoglycans. Exogenous GAGs should bind to these sites, competitively inhibit interactions with proteoglycans, and perturb development. GAGs added to the culture medium perturb the in situ growth of pioneer axons in cultured cockroach embryos by producing axon defasciculation and growth in incorrect directions. The specificity of this phenomenon is evident from the following observations: Of all the GAGs tested only heparin and heparan sulfate produced perturbation; of the six axon tracts being pioneered during the culture period only two of them are perturbed by the GAGs; and similar perturbations are produced when embryos are cultured in the presence of heparinase II and heparitinase.
authors.affiliation: Biology Department, University of Iowa, Iowa City 52242.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: J L
authors.initials: JL
authors.lastname: Denburg
mesh_terms: D000367:Age Factors; D000818:Animals; D000911:Antibodies, Monoclonal; D001369:Axons / Q000502:physiology*; D001665:Binding Sites; D006025:Glycosaminoglycans / Q000502:physiology; D006493:Heparin / Q000494:pharmacology; D006497:Heparan Sulfate / Q000502:physiology; D066298:In Vitro Techniques; D009420:Nervous System / Q000196:embryology*; D010526:Periplaneta / Q000196:embryology; D011509:Proteoglycans / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D006025:Glycosaminoglycans; D006493:Heparin; D006497:Heparan Sulfate; D011509:Proteoglycans