Association of the transformed glucocorticoid receptor with a cytoskeletal protein complex.
In a recent paper we described a system in which glucocorticoid receptors associate with particulate complexes containing tubulin [Cancer Res. 49 (1989) 2222s-2229s]. When L cell cytosol is mixed with a microtubule stabilizing buffer and heated to 37 degrees C, the receptor becomes associated with a complex that can be centrifuged out of solution at 150,000 g. In this work we show that the glucocorticoid receptor-cytoskeletal protein complex forms in a temperature and glutamate-dependent manner. Molybdate does not affect generation of the cytoskeletal protein complex but it inhibits association of the receptor with the complex. This suggests that transformation of the receptor to its DNA-binding form is required for interaction with the cytoskeletal complex. Colchicine has no effect on generation of the particulate complex or on the association of receptor with it, suggesting that formation of the complex does not represent a classic in vitro process of tubulin polymerization.
Scherrer
L C
LC
Department of Pharmacology, University of Michigan Medical School, Ann Arbor 48109.
Pratt
W B
WB
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D003078:Colchicine / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D008982:Molybdenum / Q000494:pharmacology; D011485:Protein Binding; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification / Q000378:metabolism*; D014404:Tubulin / Q000302:isolation & purification / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D011965:Receptors, Glucocorticoid; D014404:Tubulin; C044659:molybdate; D008982:Molybdenum; D003078:Colchicine