[The intercellular interactions in the interphase death of irradiated thymocytes. A study of the nature of the mediator of the interaction of irradiated thymocytes].
A study was made of the influence of cytosol and conditioned medium, obtained at different times of incubation of irradiated thymocytes, on native thymocytes, as well as of the influence of mixing the suspension and changing the medium and the enzyme inhibitors, involved in metabolism of arachidonic acid, on the radiation injury to thymocytes. Cytosol of thymocytes, incubated for 60 min after irradiation, was shown to exert an insignificant toxic effect on native thymocytes. The conditioned medium of irradiated thymocytes is virtually ineffective. The mixing of the suspension upon incubation and the changing of the medium reduced the damage to exposed thymocytes while inhibitors of phospholipase A2 and lipoxygenase prevented completely the radiation-induced pyknosis of nuclei, whereas a inhibitor of cyclooxygenase did not influence it. It is inferred from the data obtained that unstable lipoxygenase products may serve as mediators of intercellular interactions of exposed thymocytes.
Shaposhnikova
V V
VV
Dobrovinskaia
O R
OR
Eĭdus
L Kh
LKh
Korystov
Iu N
IuN
D000098:Acetophenones / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000037:antagonists & inhibitors; D002450:Cell Communication / Q000187:drug effects / Q000528:radiation effects*; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002467:Cell Nucleus / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured / Q000187:drug effects / Q000528:radiation effects; D003470:Culture Media; D003600:Cytosol / Q000187:drug effects / Q000528:radiation effects; D007213:Indomethacin / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects / Q000528:radiation effects*; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008297:Male; D009637:Masoprocol / Q000494:pharmacology; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*
D000098:Acetophenones; D003470:Culture Media; D016859:Lipoxygenase Inhibitors; D016718:Arachidonic Acid; D009637:Masoprocol; D010741:Phospholipases A; D054467:Phospholipases A2; C006821:4-bromophenacyl bromide; D007213:Indomethacin
Mezhkletochnye vzaimodeĭstviia pri interfaznoĭ gibeli obluchennykh timotsitov. Issledovanie prirody mediatora vzaimodeĭstviia obluchennykh timotsitov.