title: DNA probe for Aeromonas salmonicida.
abstract: A DNA fragment that is specific to Aeromonas salmonicida has been isolated from a genomic DNA library by differential hybridization. The specificity of this fragment as a DNA probe for A. salmonicida was shown by hybridization against reference strains and clinical isolates of A. salmonicida, related aeromonads, and species from several other bacterial genera. The sensitivity of detection by a polymerase chain reaction test, based on this fragment, was approximately two A. salmonicida cells.
authors.affiliation: Department of Microbiology, BioResearch Ireland, University College, Galway.
authors.forename: M
authors.initials: M
authors.lastname: Hiney
authors.forename: M T
authors.initials: MT
authors.lastname: Dawson
authors.forename: D M
authors.initials: DM
authors.lastname: Heery
authors.forename: P R
authors.initials: PR
authors.lastname: Smith
authors.forename: F
authors.initials: F
authors.lastname: Gannon
authors.forename: R
authors.initials: R
authors.lastname: Powell
mesh_terms: D000333:Aeromonas / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial