Functional isoforms of Pit-1 generated by alternative messenger RNA splicing.
Pit-1 is a pituitary-specific transcription factor that activates expression of the GH and PRL genes. We have isolated a cDNA encoding a variant isoform of Pit-1 that we term Pit-1 beta. Pit-1 beta contains an insertion of 26 amino acids in the transactivation domain as a consequence of the utilization of an alternative 3' splice acceptor at the end of the first intron. Like the previously described Pit-1 alpha, Pit-1 beta transactivates both the GH and PRL promoters. However, the larger isoform acts as a more potent inducer of GH.
Konzak
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Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Moore
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D013006:Growth Hormone / Q000235:genetics; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000166:cytology / Q000378:metabolism*; D010957:Plasmids; D011388:Prolactin / Q000235:genetics; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D051381:Rats; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D004268:DNA-Binding Proteins; C494186:Pou1f1 protein, rat; D050817:Transcription Factor Pit-1; D014157:Transcription Factors; D011388:Prolactin; D013006:Growth Hormone