title: Interleukin 3 directly stimulates both megakaryocyte progenitor cells and immature megakaryocytes.
abstract: A subset of stem cell antigen (sca-1)-positive mouse megakaryocyte progenitors was identified that correlates with other primitive precursors in bone marrow. The responsive bone marrow cells were obtained by depleting the marrow of cells bearing defined lineage markers (neutrophils, macrophages, and lymphoid cells) and enriched for primitive myeloid progenitor cells with high proliferative potential, selecting for cells expressing sca-1. The sca-1-positive megakaryocyte progenitors formed colonies in the presence of interleukin 3 (IL-3) alone. Immature megakaryocytes depleted of mature megakaryocytes and of cells expressing myeloid and lymphoid lineage markers were also responsive to IL-3. These data indicate that in the presence of high doses of IL-3, accessory cells are not obligatory for growth factor stimulation of megakaryocytopoiesis in vitro.
authors.affiliation: Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: H
authors.initials: H
authors.lastname: Kavnoudias
authors.forename: H
authors.initials: H
authors.lastname: Jackson
authors.forename: K
authors.initials: K
authors.lastname: Ettlinger
authors.forename: I
authors.initials: I
authors.lastname: Bertoncello
authors.forename: I
authors.initials: I
authors.lastname: McNiece
authors.forename: N
authors.initials: N
authors.lastname: Williams
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D007377:Interleukin-3 / Q000494:pharmacology*; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology
chemical_list: D007377:Interleukin-3; D011994:Recombinant Proteins