title: Abdominal surgery-induced delayed gastric emptying in rats: role of CRF and sensory neurons.
abstract: The role of peripheral corticotropin-releasing factor (CRF) and afferent pathways in mediating abdominal surgery-induced delayed gastric emptying was investigated using an intravenous injection of the competitive CRF antagonist alpha-helical CRF9-41 and capsaicin pretreatment in adult fasted rats. Gastric emptying of a non-caloric solution was measured by the phenol red method. Intravenous CRF (0.13 nmol) inhibited by 65% gastric emptying. CRF effect was completely reversed by simultaneous intravenous injection of alpha-helical CRF9-41 (13 nmol). Abdominal surgery under enflurane anesthesia inhibited gastric emptying by 60% measured 3 h postoperatively. Enflurane anesthesia alone had no effect on gastric emptying 3 h after exposure. Abdominal surgery-induced delay of gastric emptying was reversed by 46, 60, and 100% by capsaicin pretreatment (125 mg/kg, -3 wk), intravenous injection of alpha-helical CRF9-41 (13 nmol), and combined pretreatments with capsaicin and CRF antagonist, respectively. Capsaicin and alpha-helical CRF9-41 given alone or in combination did not influence gastric emptying. These results indicate that peripheral CRF receptors along with capsaicin-sensitive afferent neurons contribute to somatovisceral stress (abdominal surgery)-induced delayed gastric emptying.
authors.affiliation: Center for Ulcer Research and Education, Veterans Affairs Wadsworth Medical Center, Los Angeles 90073.
authors.forename: E
authors.initials: E
authors.lastname: Barquist
authors.forename: M
authors.initials: M
authors.lastname: Zinner
authors.forename: J
authors.initials: J
authors.lastname: Rivier
authors.forename: Y
authors.initials: Y
authors.lastname: Taché
mesh_terms: D000005:Abdomen / Q000601:surgery*; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D003346:Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology*; D005746:Gastric Emptying / Q000187:drug effects; D007275:Injections, Intravenous; D008297:Male; D009475:Neurons, Afferent / Q000502:physiology*; D010446:Peptide Fragments / Q000494:pharmacology; D011184:Postoperative Period; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D010446:Peptide Fragments; D003346:Corticotropin-Releasing Hormone; C045089:corticotropin releasing hormone (9-41); D002211:Capsaicin