A putative serine/threonine protein kinase gene on chromosome III of Saccharomyces cerevisiae.
We have sequenced a gene on chromosome III of Saccharomyces cerevisiae which codes for a putative serine/threonine protein kinase of 726 amino acids (calculated molecular weight 82 kDa). We have called this gene KIN82. The amino acid sequence of KIN82 is most similar to the cyclic nucleotide-dependent protein kinase subfamily and the protein kinase C subfamily. Gene disruption of KIN82 did not produce any phenotype when tested under a variety of conditions. Reduced stringency hybridizations revealed the presence of another genomic sequence with high homology to the carboxy-terminal catalytic domain of KIN82.
Wilson
C
C
Department of Cellular and Developmental Biology, University of Rome La Sapienza, Italy.
Bergantino
E
E
Lanfranchi
G
G
Valle
G
G
Carignani
G
G
Frontali
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009693:Nucleic Acid Hybridization; D011494:Protein Kinases / Q000145:classification / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012694:Serine / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism*
D013912:Threonine; D012694:Serine; D011494:Protein Kinases