title: New Granada Medium for detection and identification of group B streptococci.
abstract: A methotrexate-containing medium for the detection of beta-hemolytic group B streptococci from clinical specimens on the basis of detection of pigment is described. The medium contained peptone, starch, serum, MgSO4, glucose, pyruvate, methotrexate (as pigment enhancer), phosphate-morpholine-propanesulfonic acid buffer, and selective agents. The recovery of beta-hemolytic group B streptococci was comparable to that obtained with selective broth.
authors.affiliation: Microbiology Service, Virgen de las Nieves Hospital, Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: de la Rosa
authors.forename: M
authors.initials: M
authors.lastname: Perez
authors.forename: C
authors.initials: C
authors.lastname: Carazo
authors.forename: L
authors.initials: L
authors.lastname: Pareja
authors.forename: J I
authors.initials: JI
authors.lastname: Peis
authors.forename: F
authors.initials: F
authors.lastname: Hernandez
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D008727:Methotrexate; D010858:Pigmentation; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D003470:Culture Media; D008727:Methotrexate