Role of bacterial infection in diet-induced acute pancreatitis in mice.
This study was performed to elucidate the role of bacterial infection in acute pancreatitis in young female mice fed a choline-deficient diet supplemented with 0.5% DL-ethionine (CDE diet). Mice were randomly classified into two groups: one had been fed CDE diet alone (nonantibiotic group), the other was fed a CDE diet with oral administration of antibiotics (antibiotic group). Survival rates at 96 and 144 h after introduction of the CDE diet were significantly improved in the antibiotic group, 25.0 and 19.4%, respectively, as compared with 3.6 and 0% in the nonantibiotic group (p, 0.05). Aerobic and anaerobic bacterial cultures of blood, ascites, spleen, and pancreas were taken from living mice 72 h after introduction of the CDE diet. Positive bacterial growth from one or more of the specimens occurred in 29.4% of the nonantibiotic group, and in 10.0% of the antibiotic group. Mice with pancreatic necrosis had a higher positive culture rate, 62.5% in the nonantibiotic group vs 20.0% in the antibiotic group. These results suggest that reduction of intestinal flora in mice inhibits secondary infection caused by bacterial translocation and improves survival in diet-induced hemorrhagic pancreatitis. We believe the development of bacterial infection of gut origin may be a factor influencing mortality in severe pancreatitis.
Isaji
S
S
University of California, Davis, Department of Surgery, Sacramento 95817.
Suzuki
M
M
Frey
C F
CF
Ruebner
B
B
Carlson
J
J
D000208:Acute Disease; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001424:Bacterial Infections / Q000150:complications* / Q000188:drug therapy; D002794:Choline / Q000008:administration & dosage; D002796:Choline Deficiency / Q000150:complications; D004032:Diet / Q000009:adverse effects*; D004064:Digestive System / Q000382:microbiology; D005001:Ethionine / Q000008:administration & dosage; D005260:Female; D051379:Mice; D010179:Pancreas / Q000201:enzymology; D010195:Pancreatitis / Q000150:complications* / Q000209:etiology
D000900:Anti-Bacterial Agents; D002794:Choline; D005001:Ethionine