c-myc protein complex binds to two sites in human hsp70 promoter region.
We investigated the binding of partially purified (enriched) c-myc protein to the human hsp70 promoter region by band shift and ultraviolet crosslinking assays. In the hsp70 promoter region, two sites were found to be homologous to the c-myc protein complex binding sequence in the c-myc gene. These sites are located at positions -230 and -160 bases relative to the transcription initiation site, overlapping with the region reported for the regulation of hsp70 gene expression by c-myc, and upstream of other regulatory sequences including the heat shock element and the serum responsive element. The results shown here suggest that the c-myc protein complex from human HL-60 cells binds to the two sites of the region directly and sequence specifically. It is therefore suggested that the c-myc protein or protein complex contribute to the regulation of hsp70 gene expression by binding directly to the promoter region.
Taira
T
T
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Negishi
Y
Y
Kihara
F
F
Iguchi-Ariga
S M
SM
Ariga
H
H
D001345:Autoradiography; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D016259:Genes, myc; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D016271:Proto-Oncogene Proteins c-myc; D004247:DNA