Neutrophil localization following reperfusion of ischemic skin flaps.
A swine model of island latissimus dorsi myocutaneous and buttock cutaneous flaps was used to examine neutrophil localization and flap survival after 6 hours of global ischemia followed by 24 hours of reperfusion. Radioactivity from autotransfused neutrophils labeled with indium-111 enabled their localization. Radioactivity in ischemic latissimus dorsi flaps was increased by 101 +/- 30 percent over contralateral control latissimus dorsi flaps (n = 6, p = 0.01). Radioactivity in ischemic buttock flaps was increased by 142 +/- 40 percent over contralateral control buttock flaps (n = 6, p = 0.008). Despite increased neutrophil localization to ischemic flaps, the magnitude of tissue radioactivity failed to provide sufficient information to predict ischemic injury as measured by flap survival and tissue water content.
Lee
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Microsurgical Research Laboratory, Royal Victoria Hospital, Montreal, P.Q., Canada.
Kerrigan
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D000704:Analysis of Variance; D000818:Animals; D001834:Body Water / Q000502:physiology; D002465:Cell Movement; D005260:Female; D009132:Muscles / Q000098:blood supply / Q000473:pathology; D009504:Neutrophils / Q000502:physiology*; D015427:Reperfusion Injury / Q000473:pathology*; D012867:Skin / Q000098:blood supply / Q000473:pathology; D013524:Surgical Flaps / Q000473:pathology*; D016019:Survival Analysis; D013552:Swine