Human granulocyte elastase analysis on a clinical analyser (Greiner G-400) and comparison with the manual IMAC-Elastase method.
The performance of the human granulocyte elastase (EC 3.4.21.37) determination on the clinical analyser Greiner G-400 was evaluated. The detection limit of the assay is 4.68 micrograms/l. With pooled plasma samples, within-run variation (CV) ranged from 1.3 to 6.0%, between-run imprecision was 2.7 to 6.3%. Comparison with the homogenous immunoactivation manual assay using samples from 40 patients resulted in a very good correlation (r = 0.987). This suggests that the adapted instrumental method is able to replace the manual method for routine analyses.
Tsaka
T
T
Ludwig Boltzmann Institut für Pädiatrische Endokrinologie und Immunologie, Wien.
Herkner
K R
KR
Mühl
A
A
D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D010196:Pancreatic Elastase / Q000097:blood*
D010196:Pancreatic Elastase