Carbachol stimulation of triacylglycerol lipase activity in pancreatic acinar cells.
Carbachol (CCh) reduced the levels of [3H]arachidonic acid in triacylglycerol (TG) of pancreatic acinar cells. In cells prelabeled with [14C]glycerol, CCh reduced [14C]TG and increased [14C]diacylglycerol levels. Using [3H]triolein as exogenous substrate, CCh enhanced TG lipase activity 3-fold in a particulate fraction derived from intact acinar cells. These results portray a mechanism for generating diacylglycerol and arachidonic acid in exocrine pancreas involving agonist stimulation of TG hydrolysis.
Hundley
T R
TR
Department of Pharmacology & Therapeutics, School of Medicine & Biomedical Sciences, State University of New York, Buffalo 14214.
Rubin
R P
RP
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D004075:Diglycerides / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology; D014280:Triglycerides / Q000378:metabolism; D014316:Tritium
D002250:Carbon Radioisotopes; D004075:Diglycerides; D014280:Triglycerides; D014316:Tritium; D016718:Arachidonic Acid; D002217:Carbachol; D008049:Lipase; D005990:Glycerol