title: In vitro and in vivo evaluation of antibiotic diffusion from antibiotic-impregnated polymethylmethacrylate beads.
abstract: The elution of antibiotics from antibiotic-impregnated polymethylmethacrylate (PMMA) beads was measured in mongrel dogs. The antibiotics, used in mixture with Simplex cement, included cefazolin (Ancef; 4.5 g/40 g cement powder), ciprofloxacin (Cipro; 6 g/40 g powder), clindamycin (Cleocin; 6 g/40 g powder), ticarcillin (Ticar; 12 g/40 g powder), tobramycin (Nebcin; 9.8 g/40 g powder), and vancomycin (Vancocin; 4 g/40 g powder). After a pneumatic drill was used to dredge a trough in the tibia, five beads were implanted. During the next 28 days, seroma samples and serum samples were taken for antibiotic measurements. On Day 28, the dogs were killed, beads removed, and the seroma, serum, bone, and granulation tissue sampled. The results of the study showed that clindamycin, vancomycin, and tobramycin exhibited good elution characteristics and had consistently high levels in bone and granulation tissue.
authors.affiliation: Marine Biotechnical Institute, Department of Internal Medicine, University of Texas Medical Branch, Galveston 77550-2772.
authors.forename: K
authors.initials: K
authors.lastname: Adams
authors.forename: L
authors.initials: L
authors.lastname: Couch
authors.forename: G
authors.initials: G
authors.lastname: Cierny
authors.forename: J
authors.initials: J
authors.lastname: Calhoun
authors.forename: J T
authors.initials: JT
authors.lastname: Mader
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000378:metabolism*; D001842:Bone and Bones / Q000737:chemistry; D002437:Cefazolin / Q000378:metabolism; D002939:Ciprofloxacin / Q000378:metabolism; D002981:Clindamycin / Q000378:metabolism; D004058:Diffusion; D004285:Dogs; D006097:Granulation Tissue / Q000737:chemistry; D008768:Methylmethacrylates / Q000378:metabolism*; D013982:Ticarcillin / Q000378:metabolism; D014031:Tobramycin / Q000378:metabolism; D014640:Vancomycin / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D008768:Methylmethacrylates; D002981:Clindamycin; D002939:Ciprofloxacin; D014640:Vancomycin; D013982:Ticarcillin; D002437:Cefazolin; D014031:Tobramycin