Recovery of DNA from agarose gels using a modified Elutrap.
We have made a significant improvement in the electroelution device, Elutrap (Schleicher and Schuell) by substituting an agarose gel barrier, which is made from 0.6% agarose (SeaKem GTG; FMC Corporation), into the elution chamber in place of the manufacturer specified BT2 membrane. This modification substantially increases the DNA recovery from agarose gels, even in samples containing less than 1 microgram of DNA, and shortens elution times particularly for large sizes of DNA (greater than 4.4 kbp). Additionally, the gel barrier provides a reproducible quantity and quality of DNA recovery. The high quality of the eluted DNA using the modified Elutrap makes this system suitable for further DNA manipulations.
Gu
H
H
Division of Biomedical Sciences, Meharry Medical College, Nashville, TN 37208.
Wilson
D
D
Inselburg
J
J
D004247:DNA / Q000302:isolation & purification*; D003956:Dialysis / Q000295:instrumentation / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation* / Q000379:methods; D008567:Membranes, Artificial
D008567:Membranes, Artificial; D004247:DNA