The bactericidal activity of a methyl and propyl parabens combination: isothermal and non-isothermal studies.
The effect of temperature on the kill rate of Escherichia coli by methyl and propyl parabens was studied. The kill kinetics was first order. It was shown that the Arrhenius equation provided a good model for describing the relationship between the first order rate constant and the temperature. The activation energy was found to be 274 kJ/mol for exponential phase cells and 168 kJ/mol for stationary phase cells. Exponential phase cells were much more susceptible to the lethal effects of the parabens than were the stationary phase cells. For example, at 34 degrees C stationary phase cells, in chemically defined media, had a kill rate constant of 0.072/h while the corresponding value for exponential phase cells was 0.238/h. In water the rate of kill for exponential phase cells was even faster giving a rate constant of 5.25/h at 34 degrees C. Non-isothermal kinetic testing was not found to be useful for modelling bacterial kill kinetics because we could not achieve the precision required in bacterial enumeration.
Gilliland
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D
Drug Delivery Research Group, School of Pharmacy, Queen's University of Belfast, Northern Ireland.
Li Wan Po
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A
Scott
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E
D003470:Culture Media; D004338:Drug Combinations; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D007700:Kinetics; D010226:Parabens / Q000494:pharmacology*; D013696:Temperature
D003470:Culture Media; D004338:Drug Combinations; D010226:Parabens; C015358:methylparaben; C006068:propylparaben