Purification of restriction endonuclease EcoO128I produced by an enteropathogenic Escherichia coli O128Ly3.
Restriction endonuclease EcoO128I, an isoschizomer of BstEII, was purified from a rough mutant of Escherichia coli O128Ly3. EcoO128I should be more convenient for recombinant DNA applications than BstEII, because of its improved cleavage activity at 37 degrees C.
Miyahara
M
M
Division of Microbiology, National Institute of Hygienic Sciences, Tokyo, Japan.
Mise
K
K
Kimizuka
F
F
Matsumoto
H
H
Terawaki
Y
Y
D001483:Base Sequence; D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D007700:Kinetics; D013379:Substrate Specificity
D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific; C032823:GGTNACC-specific type II deoxyribonucleases