Characterization of a murine immunoglobulin VH gene segment in subgroup III: a new member of the 7183 gene family.
A new gene for the variable region of the immunoglobulin heavy chain (VH gene) has been isolated from BALB/c adult liver DNA using a cDNA plasmid probe containing a mouse VH sequence. The complete nucleotide sequence of this germline gene (VH10-19), shows that it belongs to the 7183 gene family. The VH gene appears to contain an intervening 104-base-long sequence and displays the same recombination signal sequences that those observed in the germline 81X. The presence of an internal heptamer at the 3' end of the VH10-19 coding region let an alternative recombination event that could increase the representation of this gene in the immature repertoire.
Sequeira
A
A
Unité d'Immunocytochimie, CNRS URA 359, Institut Pasteur, Paris, France.
Jouvin-Marche
E
E
Avrameas
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes; D015723:Gene Library; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains