Comparison of cysteine-proteinases inhibitory properties of human high-molecular-weight-kininogen (HMrK) purified from plasma and serum.
We have purified both plasma and serum High Molecular Weight Kininogens (HMrK) and studied their inhibitory properties against members of the cysteine proteinases super-family. The clotting pathway liberates two different fragments by limited proteolysis of HMrK. The inhibitory properties of these fragments against the cysteine proteinases of the papain super-family are conserved after blood clotting, i.e. when the procoagulant domain of HMrK was destroyed.
Pagano
M
M
Laboratoire de Biochimie, Faculté de Medecine, Broussais Hotel-Dieu, Université Pierre et Marie Curie, Paris, France.
D001777:Blood Coagulation / Q000502:physiology*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D006801:Humans; D007704:Kininogens / Q000097:blood / Q000302:isolation & purification / Q000494:pharmacology*
D015853:Cysteine Proteinase Inhibitors; D007704:Kininogens