title: The influence of phospholipid polar head on the lipid hydroperoxide dependent initiation of lipid peroxidation.
abstract: In a buffer (Mes) and at a pH (6.5) where Fe2+ is very stable, we have studied the peroxidation of liposomes catalyzed by FeCl2. The liposomes studied, prepared by sonolysis, contained either phosphatidylcholine or 1:1 molar ratio of phosphatidylcholine and phosphatidic acid. The presence of the negatively charged phospholipid causes: 1) rapid Fe2+ oxidation and oxygen consumption; 2) increased generation of lipid hydroperoxides; 3) decreased generation of thiobarbituric acid-reactive materials; 4) very low inhibition of Fe2+ oxidation and lipid hydroperoxide generation by BHT; 5) inhibition of the termination phase of lipid peroxidation at high FeCl2 concentrations. A hypothesis is proposed to explain the results obtained.
authors.affiliation: Dipartimento di Biochimica, Università di Bologna, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Tadolini
authors.forename: D
authors.initials: D
authors.lastname: Fiorentini
authors.forename: P
authors.initials: P
authors.lastname: Motta
authors.forename: L
authors.initials: L
authors.lastname: Cabrini
authors.forename: A M
authors.initials: AM
authors.lastname: Sechi
mesh_terms: D005296:Ferrous Compounds / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008081:Liposomes; D010713:Phosphatidylcholines / Q000494:pharmacology; D010718:Phosphatidylserines / Q000494:pharmacology; D010743:Phospholipids / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D005296:Ferrous Compounds; D008081:Liposomes; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D010743:Phospholipids