Promoting effects of cigarette smoke on the respiratory tract carcinogenesis of Syrian golden hamsters treated with diethylnitrosamine.
The potential short-term promoting effects of cigarette smoke on the development of tumors in the respiratory system were investigated in male Syrian golden hamsters. Three groups (1, 2 and 3) of 30 animals each received a single s.c. injection of 100 mg/kg body wt of diethylnitrosamine (DEN) at the commencement of experiment 1. They were then exposed to non-filter cigarette (NC) smoke, filter-tip cigarette (FC) smoke and sham smoke respectively, in a Hamburg II type smoking machine from week 1 to week 12. In addition, groups 4, 5 and 6 (10 animals each) were exposed to the NC smoke, FC smoke or sham smoke respectively, for the same time period without prior DEN treatment. In the DEN-treated groups, epithelial hyperplasias and/or papillomas were induced, the incidences and numbers/animal of these lesions in groups 1 and 2 being significantly increased as compared to group 3 values. In experiment 2, two groups of 25 hamsters each were exposed to cigarette smoke or sham smoke for up to 12 weeks, five animals in each group being killed for immunohistochemical analysis using BrdU antibodies and measurement of lipid peroxides in the lung and serum at weeks 1, 2, 4, 8 and 12. Small aggregations of macrophages (smoke cells) in the lung alveoli was observed in the smoke-exposed group, but no significant increase in the numbers of BrdU positive cells in any compartment of the respiratory system was apparent. Animals of this group showed a tendency for increased lung malondialdehyde levels at weeks 2 and 12, but not weeks 4 and 8.
Takahashi
M
M
Division of Pathology, National Institute of Hygienic Sciences, Tokyo, Japan.
Imaida
K
K
Mitsumori
K
K
Okamiya
H
H
Shinoda
K
K
Yoshimura
H
H
Furukawa
F
F
Hayashi
Y
Y
D000818:Animals; D006224:Cricetinae; D004052:Diethylnitrosamine; D006965:Hyperplasia; D008054:Lipid Peroxides / Q000032:analysis; D008168:Lung / Q000737:chemistry; D008297:Male; D008647:Mesocricetus; D010212:Papilloma / Q000209:etiology; D012137:Respiratory System / Q000187:drug effects / Q000473:pathology; D012142:Respiratory Tract Neoplasms / Q000209:etiology*; D012907:Smoking / Q000009:adverse effects*
D008054:Lipid Peroxides; D004052:Diethylnitrosamine