Effects of heparan-sulphate administration on clotting parameters and serum thromboxane B2 levels in cholesterol fed rabbits.
We evaluated the effects of heparan-sulphate administration on clotting times, thromboelastographic parameters and serum thromboxane B2 levels in hypercholesterolemic rabbits with aortic atherosclerotic lesions (sudanophilic areas). 24 New Zealand male rabbits were divided into three groups of 8 animals each. Group A and B were fed a rabbit chow diet containing 0.7% of cholesterol whereas Group C was fed a standard rabbit diet without cholesterol. Group A was treated by subcutaneous route with 6 mg/kg/day of heparan sulphate. At the beginning of the study and after 3 and 6 months of treatment, serum cholesterol and thromboxane B2 levels were tested. Furthermore, at the end of the experiment, we evaluated plasma fibrinogen, aPTT, PT and TT values. The administration of heparan-sulphate in cholesterol fed rabbits produced: a reduction of plasma fibrinogen levels, without modifying aPTT and TT; a protective effect vs the lengthening in PT values, likely induced by cholesterol rich diet; a reduction of plasma thrombophilic activities and of aortic atheromasic involvement induced by dietetic cholesterol intake. However, increased serum thromboxane B2 levels, likely through a proaggregant activity were observed. We suggest that heparan-sulphate administration, in cholesterol fed rabbits, has a favourable effect on clotting parameters, while contrasting effects were found on platelet activity.
Restori
G
G
Dipartimento di Medicina interna, Università, Parma.
Boiardi
L
L
Manotti
C
C
Carpi
L
L
Bonicauri
E
E
Cerletti
C
C
Ferrari
G
G
Delsignore
R
R
D000818:Animals; D001161:Arteriosclerosis / Q000097:blood / Q000188:drug therapy; D001777:Blood Coagulation / Q000187:drug effects*; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D004353:Drug Evaluation, Preclinical; D006497:Heparan Sulfate / Q000627:therapeutic use*; D006937:Hypercholesterolemia / Q000097:blood / Q000188:drug therapy; D008297:Male; D011817:Rabbits; D013929:Thromboxane B2 / Q000097:blood*; D013997:Time Factors
D002791:Cholesterol, Dietary; D006497:Heparan Sulfate; D013929:Thromboxane B2