title: Sequence and characterization of pvuIIR, the PvuII endonuclease gene, and of pvuIIC, its regulatory gene.
abstract: An open reading frame partially overlaps pvuIIR, and genetic evidence implies that this open reading frame, named pvuIIC, specifies a positive regulator of pvuIIR (T. Tao, J. C. Bourne, and R. M. Blumenthal, J. Bacteriol. 173:1367-1375, 1991). Inducible constructs of pvuIIC produced a protein of the expected size. The site of C.PvuII action appears to lie within pvuIIC itself; thus, pvuIIC may be a self-contained regulatory cassette.
authors.affiliation: Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.
authors.forename: T
authors.initials: T
authors.lastname: Tao
authors.forename: R M
authors.initials: RM
authors.lastname: Blumenthal
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005809:Genes, Regulator / Q000235:genetics*; D008969:Molecular Sequence Data; D011514:Proteus vulgaris / Q000235:genetics*; D011994:Recombinant Proteins
chemical_list: D011994:Recombinant Proteins; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific