Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor.
The crystal structure of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) has been determined at 2.8 A resolution using multiple isomorphous replacement techniques. There are two molecules in the crystallographic asymmetric unit, which are related by an approximate non-crystallographic 2-fold axis. The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhGM-CSF is a four alpha-helix bundle, which represents approximately 42% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within the connections is a two-stranded antiparallel beta-sheet. The tertiary structure of rhGM-CSF has a topology similar to that of porcine growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus.
Walter
M R
MR
Department of Pathology, University of Alabama, Birmingham 35294.
Cook
W J
WJ
Ealick
S E
SE
Nagabhushan
T L
TL
Trotta
P P
PP
Bugg
C E
CE
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003196:Computer Graphics; D003461:Crystallography; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000648:ultrastructure*; D006801:Humans; D006922:Hylobates; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins; D016415:Sequence Alignment; D014961:X-Ray Diffraction
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor