title: Differential effects of human blood monocytes on the growth of human tumour cell lines in vitro.
abstract: Monocytes and macrophages have been shown to be cytotoxic towards tumour cells in vitro. However, although tumour-associated monocytes and macrophages are now widely accepted to contribute a relatively high proportion of the cellular infiltrate of experimental and human solid carcinomas, a cytotoxic/cytostatic effector function for these cells in vitro or in vivo has yet to be conclusively demonstrated. In the present study, we show that non-activated peripheral blood monocytes co-cultured with tumour cells across a semi-permeable membrane release soluble factors that modulate the growth of tumour cells in contrasting ways. After Nycoprep 1.068 separation, non-activated peripheral blood monocytes enhanced the in vitro proliferation of HT29 colon adenocarcinoma cells but inhibited T47D breast carcinoma cell replication; peripheral blood lymphocytes were incapable of mediating these effects. In contrast, peripheral blood monocytes activated by interferon gamma caused a pronounced inhibition of both HT29 and T47D cell proliferation.
authors.affiliation: Department of Experimental and Clinical Microbiology, University of Sheffield Medical School, South Yorkshire, UK.
authors.forename: H J
authors.initials: HJ
authors.lastname: Parry
authors.forename: R C
authors.initials: RC
authors.lastname: Rees
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002463:Cell Membrane Permeability; D002470:Cell Survival; D003110:Colonic Neoplasms / Q000473:pathology; D016207:Cytokines / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008214:Lymphocytes / Q000502:physiology; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D009374:Neoplasms, Experimental / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000166:cytology
chemical_list: D016207:Cytokines; D007371:Interferon-gamma