Stereochemical analysis of betamethasone and dexamethasone by derivatization and high-performance liquid chromatography.
A simple and economical high-performance liquid chromatographic method has been developed for the simultaneous determination of betamethasone and dexamethasone. The method is based on the derivatization of the structural epimers of betamethasone and dexamethasone with a homochiral reagent, N-carbobenzoxy-L-phenylalanine. The derivatives obtained were easily recognized by a non-chiral silica column with n-hexane-dichloromethane-isopropanol (100:100:4, v/v/v) as a mobile phase and a good separation was obtained for quantitation. The method was satisfactorily applied to the determination of betamethasone and dexamethasone in tablets.
Chen
S H
SH
Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical College, Taiwan.
Wu
S M
SM
Wu
H L
HL
D001623:Betamethasone / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003907:Dexamethasone / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013237:Stereoisomerism
D003907:Dexamethasone; D001623:Betamethasone