title: Treatment of leg ulcers with cryopreserved allogeneic cultured epithelium. A multicenter study.
abstract: BACKGROUND
  In the past few years, several authors have described the usefulness of cultured allogeneic epidermal sheets in promoting wound healing of burns, leg ulcers, and donor sites. This study reports clinical results obtained by different departments in the treatment of chronic leg ulcers by cryopreserved cultured allogeneic epithelium. The freezing procedure and the assessment of viability of the cryopreserved epithelium are also described. A total of 30 ulcers were treated using 138 cryopreserved allografts.
  
  
  METHODS
  Twenty ulcers (66.6%) healed completely within 12 weeks. Four ulcers showed a 30% to 84.4% reduction in size by 3 weeks but did not heal completely; the remaining six ulcers did not show any improvement. A strong stimulation of granulation tissue formation and of reepithelialization from the wound edge were observed.
  
  
  RESULTS
  The results indicate that frozen cultured epidermis, stored in a skin bank, is a valid and generally applicable alternative therapy for the treatment of chronic ulcers.
authors.affiliation: National Institute for Cancer Research, Laboratory of Cell Differentiation, Genoa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: De Luca
authors.forename: E
authors.initials: E
authors.lastname: Albanese
authors.forename: R
authors.initials: R
authors.lastname: Cancedda
authors.forename: A
authors.initials: A
authors.lastname: Viacava
authors.forename: A
authors.initials: A
authors.lastname: Faggioni
authors.forename: G
authors.initials: G
authors.lastname: Zambruno
authors.forename: A
authors.initials: A
authors.lastname: Giannetti
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002478:Cells, Cultured; D002908:Chronic Disease; D015925:Cryopreservation; D004847:Epithelial Cells; D004848:Epithelium / Q000637:transplantation; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D007871:Leg Ulcer / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D012074:Remission Induction