title: Preferential hydroxylation by the chemical nuclease meso-tetrakis-(4-N-methylpyridiniumyl)porphyrinatomanganeseIII pentaacetate/KHSO5 at the 5' carbon of deoxyriboses on both 3' sides of three contiguous A.T base pairs in short double-stranded oligonucleotides.
abstract: Selected double-stranded oligodeoxyribonucleotides have been used to probe, at the molecular level, DNA chain breakages induced by the chemical nuclease mesotetrakis(4-N- methylpyridiniumyl)porphyrinatomanganeseIII pentaacetate/KHSO5. The results show that cleavage selectively occurs on the two 3' sides of three contiguous A.T base pairs (an A.T triplet). Hydroxylation at 5' carbon of the deoxyribose targets represents the initial damage on the sugar-phosphodiester backbone and leaves a 3' phosphate and a 5' aldehyde at the ends. The fragments were separated by HPLC and unambiguously identified through chemical and biochemical reactions and/or sequencing after enzymatic conversion to mononucleosides. Also studied was the degradation of a 22-nucleotide DNA molecule containing two A.T triplets. Gel electrophoresis analyses on the corresponding 5'-32P-end-labeled substrate supported the above cleavage specificity and mechanism.
authors.affiliation: Laboratoire de Chimie de Coordination, Centre National de la Recherche Scientifique, Toulouse, France.
authors.forename: M
authors.initials: M
authors.lastname: Pitié
authors.forename: G
authors.initials: G
authors.lastname: Pratviel
authors.forename: J
authors.initials: J
authors.lastname: Bernadou
authors.forename: B
authors.initials: B
authors.lastname: Meunier
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003855:Deoxyribose / Q000737:chemistry; D006868:Hydrolysis; D006900:Hydroxylation; D008665:Metalloporphyrins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship
chemical_list: D008665:Metalloporphyrins; D009838:Oligodeoxyribonucleotides; D003855:Deoxyribose; C050950:tetrakis(N-methyl-4-pyridiniumyl)porphine manganese(III) complex