title: Distribution patterns and frequency of proliferating cells in cutaneous keratinocytic neoplasms. Immunohistochemical study with a monoclonal antibody (TOB7) used against proliferating cell nuclear antigen.
abstract: BACKGROUND
  Almost all markers for proliferating cells need freshly frozen tissues for evaluation; therefore retrospective study is impossible.
  
  
  OBJECTIVE
  In the present study, a murine monoclonal antibody (TOB7) against the proliferating cell nuclear antigen (PCNA) was used for the analysis of cell kinetics of cutaneous keratinocytic neoplasms. The antibody is applicable to formalin-fixed, paraffin-embedded tissues.
  
  
  METHODS
  The frequency of PCNA-positive cells and their distribution patterns were immunohistochemically investigated in various cutaneous keratinocytic neoplasms.
  
  
  RESULTS
  Squamous cell carcinoma and Bowen's disease showed significantly increased numbers of PCNA-positive cells when compared with other keratinocytic neoplasms. A characteristic marginal or random distribution pattern of PCNA-positive cells was observed in the lesions of each disease category.
  
  
  CONCLUSIONS
  Important information on the growth dynamics of keratinocytic neoplasms was obtained in this retrospective immunohistochemical study.
authors.affiliation: Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Saida
authors.forename: S
authors.initials: S
authors.lastname: Dohi
authors.forename: M
authors.initials: M
authors.lastname: Sadaki
authors.forename: Y
authors.initials: Y
authors.lastname: Tokuda
authors.forename: S
authors.initials: S
authors.lastname: Ikegawa
authors.forename: Y
authors.initials: Y
authors.lastname: Takasaki
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology*; D002455:Cell Division; D002467:Cell Nucleus / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology*; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000453:epidemiology / Q000378:metabolism / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor