Effects of gastrin on cytosolic free Ca2+ in individual, acid-secreting rat parietal cells.
The effects of gastrin on cytosolic free Ca2+ ([Ca2+]i) in single, isolated rat gastric parietal cells were investigated using the fluorescent probe Fura-2 and digital image analysis. [Ca2+]i was increased by gastrin (100 nM) in approximately 30% of the parietal cells, which were identified by using either the fluorescent probe acridine orange or a parietal cell-specific monoclonal antibody. In the dominant pattern observed, [Ca2+]i was elevated 50-150% and returned within 1-2 min to a value 30-60% over the basal, which was sustained until withdrawal of the stimulant or addition of the gastrin inhibitor L-365,260 (1 microM). The second, but not the first phase, was abolished in the absence of extracellular Ca2+. The results indicate the existence of functional gastrin receptors in a subpopulation of rat parietal cells.
Cabero
J L
JL
Department of Medical and Physiological Chemistry, Uppsala, Sweden.
Grapengiesser
E
E
Gylfe
E
E
Li
Z Q
ZQ
Mårdh
S
S
D000143:Acids / Q000378:metabolism; D000165:Acridine Orange; D000818:Animals; D000911:Antibodies, Monoclonal; D001570:Benzodiazepinones / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D016257:Fura-2; D005755:Gastrins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006632:Histamine / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000378:metabolism*; D010671:Phenylurea Compounds; D051381:Rats
D000143:Acids; D000911:Antibodies, Monoclonal; D001570:Benzodiazepinones; D005755:Gastrins; D010671:Phenylurea Compounds; C058121:L 365260; D006632:Histamine; D002217:Carbachol; D000165:Acridine Orange; D002118:Calcium; D016257:Fura-2