Growth hormone control of tissue protein metabolism in dwarf mice: enhancement by a monoclonal antibody.
Monoclonal antibody (MAb) to GH has been shown to increase the anabolic response induced by the hormone in individual tissues of dwarf mice. Dwarf mice were treated with GH at a low and a high dose (2.5 and 50 mU/day respectively), with and without complexing to an MAb. Treatment was for 7 and 14 days, at which times protein synthesis rates in skeletal muscle, liver and heart were determined from incorporation of labelled phenylalanine following injection of a flooding dose. The MAb potentiated the actions of GH and produced increases in the rates of protein synthesis in each of the tissues to a significantly greater extent than did GH alone. The increase in protein synthesis rate induced by MAb appears to be mechanistically distinct from that observed by increasing the dose of GH. In skeletal muscle and liver there was a dose-response to the GH alone in terms of the RNA concentration, i.e. the capacity for protein synthesis, whereas in each tissue examined the MAb caused very little further response in the RNA concentration. The MAb-induced enhancement of protein synthesis rate was almost entirely due to an increase in the RNA activity, i.e. the efficiency of the synthesizing system. Complexing GH to a particular MAb, or to antisera of restricted epitope specificity, has previously been shown to enhance the in-vivo effects of GH on whole body protein content; the mechanism for this enhancement has not been adequately determined.(ABSTRACT TRUNCATED AT 250 WORDS)
Bates
P C
PC
Nutrition Research Unit, London School of Hygiene and Tropical Medicine, U.K.
Aston
R
R
Holder
A T
AT
D000818:Animals; D000911:Antibodies, Monoclonal; D002356:Cartilage / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D013006:Growth Hormone / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D051379:Mice; D009124:Muscle Proteins / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
D000911:Antibodies, Monoclonal; D009124:Muscle Proteins; D011506:Proteins; D013431:Sulfates; D013006:Growth Hormone