title: Prediction and Fourier transform infrared spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-beta-1,3-1,4-D-glucanase.
abstract: The secondary structure of a recombinant Bacillus licheniformis endo-beta-1,3-1,4-D-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% beta-sheet, 25% reverse turn, 24% non-ordered and 11% alpha-helix. From theoretical prediction of secondary structure the protein would present 37% beta-sheet, 31% reverse turn, 22% non-ordered and 10% alpha-helix.
authors.affiliation: Institut de Biologia Fonamental, Facultat de Ciencies, Universitat Autonoma de Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Querol
authors.forename: E
authors.initials: E
authors.lastname: Padrós
authors.forename: M
authors.initials: M
authors.lastname: Juncosa
authors.forename: A
authors.initials: A
authors.lastname: Planas
authors.forename: J
authors.initials: J
authors.lastname: Lloberas
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D005583:Fourier Analysis; D006026:Glycoside Hydrolases / Q000737:chemistry*; D008961:Models, Structural; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D013055:Spectrophotometry, Infrared
chemical_list: D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C028020:licheninase