[The value of flow cytometry in the prognosis and treatment of head-and-neck cancers. Preliminary studies].
There is no reliable method to determine the malignancy degree of a tumour. Histology is not accurate. TNM classification is very interesting for planning surgical technique but gives little information about cancer aggressiveness. Flow-cytometry is a new technique to measure nuclear DNA quantity with great reproducibility and sensitivity. A normal cell is diploid (2N chromosome) and a tumoral one aneuploid. DNA quantitation is possible on fresh as well as on histological samples stored in formaldehyde or paraffin. Aneuploidy reflects the cancer aggressiveness. Aneuploidy is also correlated with TNM classification. Aneuploid tumors could require a more "aggressive" treatment regardless their size.
Remacle
M
M
Service O.R.L. et Chirurgie Cervico-Faciale, Université de Louvain.
Levie
P
P
Chatelain
B
B
D004273:DNA, Neoplasm / Q000737:chemistry* / Q000235:genetics; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D011003:Ploidies; D011379:Prognosis; D012680:Sensitivity and Specificity
D004273:DNA, Neoplasm
L'intérêt de la cytométrie de flux pour le pronostic et le traitement des cancers de la tête et du cou. Etude préliminaire.