The genome size and the structure and content of ribosomal RNA genes in Atlantic cod (Gadus morhua L.).
1. The haploid genome size of the Atlantic cod was estimated to 3.4 x 10(8)kb by reassociation kinetics analysis of cod sperm DNA. 2. The size of the small and large subunit ribosomal RNAs is 1.85 and 4.1 kb, respectively. 3. Restriction enzyme mapping of the rRNA coding unit revealed conservation of an Eco RI site in the coding regions of 18 S and 28 S rRNA and a Bam HI site in the 28 S rRNA coding region compared to other fish species. 4. The length of the repeat unit of the cod rDNA was found to be 30 kb. 5. The rRNA genes are repeated approximately 50 times in the cod genome and constitutes 0.08% of the cod genetic material.
Grøsvik
B E
BE
University of Bergen, Department of Biochemistry, Norway.
Raae
A J
AJ
D000818:Animals; D015139:Blotting, Southern; D004275:DNA, Ribosomal; D005399:Fishes / Q000235:genetics*; D016678:Genome; D007700:Kinetics; D008297:Male; D012335:RNA, Ribosomal / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal