Evaluation of phagocytic function of canine peripheral polymorphonuclear leucocytes by whole blood chemiluminescence.
Zymosan-induced and luminol-aided chemiluminescence (CL) of whole blood from beagle dogs was estimated for the function of polymorphonuclear leucocytes (PMNs). Whole blood (0.1 ml) was examined directly and results were obtained within 20 min. A phagocytic function of PMNs can be estimated from the peak CL counts and the number of PMNs in a specimen, and the opsonic activity can also be estimated by the peak time showing peak CL after the addition of non-opsonized zymosan. The optimal temperatures to keep diluted whole blood for the CL measurement was around 13 degrees C. Thus, this method offers information concerning the functions of phagocytic cells in whole blood.
Makimura
S
S
Department of Veterinary Internal Medicine, Faculty of Agriculture, Miyazaki University, Japan.
Sawaki
M
M
D000818:Animals; D001793:Blood Preservation; D002374:Catalase / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs / Q000097:blood / Q000276:immunology*; D000431:Ethanol / Q000494:pharmacology; D016166:Free Radical Scavengers; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D010587:Phagocytosis; D013482:Superoxide Dismutase / Q000494:pharmacology; D013696:Temperature
D016166:Free Radical Scavengers; D000431:Ethanol; D002374:Catalase; D013482:Superoxide Dismutase