title: Purification of the DNA-dependent RNA polymerase from the myxobacterium Stigmatella aurantiaca.
abstract: The DNA-dependent RNA polymerase (EC 2.7.7.6) of the myxobacterium Stigmatella aurantiaca has been purified. It shows three main polypeptide bands with apparent molecular weights of 146,000, 105,000, and 40,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. beta and beta' subunits of the S. aurantiaca polymerase were shown to migrate in the 146,000-molecular-weight polypeptide band and the main sigma factor was shown to migrate in the 105,000-molecular-weight band by using heterologous antisera.
authors.affiliation: Zentrum für Molekulare Biologie, Universität Heidelberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Heidelbach
authors.forename: H
authors.initials: H
authors.lastname: Skladny
authors.forename: H U
authors.initials: HU
authors.lastname: Schairer
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008970:Molecular Weight; D009231:Myxococcales / Q000235:genetics*; D013045:Species Specificity
chemical_list: D046911:Macromolecular Substances; D012321:DNA-Directed RNA Polymerases