title: Stereoselective high-performance liquid chromatographic determination of flurbiprofen in human plasma.
abstract: An enantioselective high-performance liquid chromatographic assay for the quantitation of the enantiomers of flurbiprofen in human plasma is described. The procedure involved extraction of flurbiprofen from acidified plasma into hexane-diethyl ether (8:2, v/v). Stereoselective separation was achieved with a prepacked alpha 1-acid glycoprotein column without any derivatization procedure. A second assay using a conventional reversed-phase column to determine racemic flurbiprofen is also described. The detection wavelength was set at 246 nm. The limit of quantification was found to be 50 ng/ml for both assays. The method was demonstrated to be sufficiently sensitive for stereoselective pharmacokinetic studies of flurbiprofen.
authors.affiliation: Department of Pharmacology and Toxicology, University of Erlangen-Nürnberg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Geisslinger
authors.forename: S
authors.initials: S
authors.lastname: Menzel-Soglowek
authors.forename: O
authors.initials: O
authors.lastname: Schuster
authors.forename: K
authors.initials: K
authors.lastname: Brune
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005480:Flurbiprofen / Q000097:blood*; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D005480:Flurbiprofen