Recovery of proteins from the broncho-alveolar lavage fluid proposal for a standardisation.
In this study various precipitation methods have been used to concentrate the proteins from broncho-alveolar-lavages. The highest percentage of proteins was recovered from the broncho-alveolar-lavages using the method of Wessel & Flügge (Anal. Biochem. 138 (1984) 141-143). The recovered proteins were further analysed by SDS-polyacrylamide gel electrophoresis. Except for low molecular mass proteins, the method of Wessel & Flügge proved to be the most effective for the recovery of individual proteins. In general, the method of Wessel & Flügge seems to be the superior method for concentrating proteins of broncho-alveolar-lavages for further analysis.
Schumacher
U
U
Anatomische Anstalt, Ludwig Maximilians Universität München.
Mausolf
A
A
Barth
J
J
Welsch
U
U
Petermann
W
W
D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D012995:Solubility
D011506:Proteins