title: Automated column equilibration, washing, sample loading and elution of bench-packed mini-columns.
abstract: In this paper we describe an easy and efficient way to pack mini-columns and use them in the Biomek 1000 Automated Laboratory Workstation. These procedures allow for automated column equilibration, column washing, sample loading and elution, and utilization of commercially available components. Various resins can be packed, allowing for automation of a variety of procedures in which minicolumns are used.
authors.affiliation: Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
authors.forename: P
authors.initials: P
authors.lastname: Jagadeeswaran
mesh_terms: D001331:Automation / Q000295:instrumentation / Q000379:methods; D001709:Biotechnology; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000302:isolation & purification; D005069:Evaluation Studies as Topic
chemical_list: D004247:DNA