Identification, molecular characterization and immunolocalization of an isoform of the trans-Golgi-network (TGN)-specific integral membrane protein TGN38.
TGN38 is an integral membrane protein previously shown to be predominantly localized to the trans-Golgi network (TGN) of cells by virtue of a signal contained within its cytoplasmic 'tail' [Luzio, Brake, Banting, Howell, Braghetta & Stanley (1990) Biochem. J. 270, 97-102]. We now (i) describe the isolation of cDNA clones encoding an isoform of TGN38, (ii) present the sequence of that isoform and (iii) describe the production and use of antibodies which specifically recognize the new isoform. This isoform, designated TGN41, is also predominantly localized to the TGN. The only sequence differences between the protein coding regions of cDNA clones encoding TGN38 and those encoding TGN41 occur within the region specifying the cytoplasmic tails of the two proteins. The TGN localization signal is shown to be within the sequence common to both proteins.
Reaves
B
B
Department of Biochemistry, School of Medical Sciences, University of Bristol, U.K.
Wilde
A
A
Banting
G
G
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D007106:Immune Sera; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D000906:Antibodies; D006023:Glycoproteins; D007106:Immune Sera; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D010455:Peptides; C513045:Tgoln2 protein, rat