title: High-performance liquid chromatography of sulphonamides extracted from bovine and porcine muscle by solid-phase dispersion.
abstract: A method has been developed for the analysis of sulphonamides in bovine and porcine muscle, based on solid-phase dispersion. Muscle tissue was blended with pre-washed C18 coated silica (55-105 microns), and the resulting homogeneous solid packed into a polypropylene syringe barrel. Fatty material was washed from the sample using hexane, and the sulphonamide analytes eluted with dichloromethane. The collected fraction was dried under nitrogen and reconstituted in 20% methanol in 0.01 M sodium acetate/acetic acid (pH 5) buffer. After sonication and filtration, the sample was analysed by high-performance liquid chromatography on a C18 column using UV diode array detection. Individual sulphonamides could be detected down to 0.01 ppm, whilst analyte identity could be confirmed by diode array spectrum down to 0.02 ppm.
authors.affiliation: Regional Veterinary Laboratory, Department of Agriculture, Hamilton, Victoria, Australia.
authors.forename: L V
authors.initials: LV
authors.lastname: Walker
authors.forename: J R
authors.initials: JR
authors.lastname: Walsh
authors.forename: J J
authors.initials: JJ
authors.lastname: Webber
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D009132:Muscles / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000032:analysis*; D013552:Swine
chemical_list: D013449:Sulfonamides