Localization of immunoglobulin-containing cells in the abomasum of sheep following infection with Haemonchus contortus.
Abomasal cannulation followed by serial collection of biopsies was used to study the kinetics of appearance of IgA-, IgG1-, IgG2- and IgM-containing cells in the abomasum of sheep following infection with Haemonchus contortus. Very few immunoglobulin-containing cells (ICC) were found in the abomasum of sheep before infection. Following H. contortus infection, there were increased numbers of ICC in the submucosa of the abomasum. Seven days after infection, the numbers of IgA-, IgG1- and IgM-containing cells were six times greater than for uninfected control animals. The numbers of ICC continued to rise as the infection progressed, and the peak response was observed between 21 and 28 days after infection. IgA-containing cells (68-84%) were the most frequent cell types at all the observation times, followed by IgG1 and IgM. IgG2-containing cells were minimal throughout the experiment. As there were no significant changes in the numbers of ICC in the abomasum of uninfected controls it is concluded that H. contortus stimulated a local immune response in the abomasum of parasitized sheep.
Gill
H S
HS
Department of Animal Science, University of New England, Armidale, NSW, Australia.
Husband
A J
AJ
Watson
D L
DL
D000018:Abomasum / Q000276:immunology* / Q000469:parasitology; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000921:Antibody-Producing Cells / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006188:Haemonchiasis / Q000276:immunology / Q000662:veterinary*; D006189:Haemonchus / Q000276:immunology*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
D000909:Antibodies, Helminth