title: Neuromedin B is present in lung cancer cell lines.
abstract: Previously, high levels of gastrin-releasing peptide and its mRNA were detected in classic small cell lung cancer cell lines. Here the ability of lung cancer cell lines to synthesize neuromedin B (NMB), a structurally similar mammalian bombesin-like peptide, was investigated. By radioimmunoassay, NMB (0.1-0.7 pmol/mg of protein) was detected in 23 of 33 lung cancer cell lines. In contrast, gastrin-releasing peptide (0.1-12.9 pmol/mg of protein) was detected in 16 of 32 cell lines. Using gel filtration and high pressure liquid chromatography techniques, the main peak of immunoreactive NMB coeluted with synthetic NMB. By Northern analysis, a 0.8-kilobase mRNA species was present, using poly(A) mRNA derived from two of three lung cancer cell lines. Using a more sensitive S1 nuclease protection assay, NMB mRNA was present in most of the 15 lung cancer cell lines examined. These data suggest that NMB may be a regulatory peptide in lung cancer.
authors.affiliation: NCI-Navy Medical Oncology Branch, National Cancer Institute, Bethesda, Maryland.
authors.forename: G
authors.initials: G
authors.lastname: Giaccone
authors.forename: J
authors.initials: J
authors.lastname: Battey
authors.forename: A F
authors.initials: AF
authors.lastname: Gazdar
authors.forename: H
authors.initials: H
authors.lastname: Oie
authors.forename: M
authors.initials: M
authors.lastname: Draoui
authors.forename: T W
authors.initials: TW
authors.lastname: Moody
mesh_terms: D015152:Blotting, Northern; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D015287:Neurokinin B / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D011863:Radioimmunoassay; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D015287:Neurokinin B; C038393:neuromedin B