Inserting foreign peptides into the major coat protein of bacteriophage M13.
Foreign DNA fragments were inserted into filamentous phage gene VIII to create hybrid B-proteins with foreign sequences in the amino terminus. The hybrid proteins are incorporated into the virions which retain viability and infectivity. Virions with hybrid B-proteins have the same contour length and the same number of B-protein molecules as virions with natural B-proteins. It was shown that for one of hybrid B-proteins the position of the processing site had changed.
Ilyichev
A A
AA
Research and Technology Institute of Biologically Active Substances, Vektor NPO, Berdsk, Novosibirsk, Region, USSR.
Minenkova
O O
OO
Kishchenko
G P
GP
Tat'kov
S I
SI
Karpishev
N N
NN
Eroshkin
A M
AM
Ofitzerov
V I
VI
Akimenko
Z A
ZA
Petrenko
V A
VA
Sandakhchiev
L S
LS
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics* / Q000472:pathogenicity; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010455:Peptides / Q000235:genetics*; D010957:Plasmids
D010455:Peptides