A specific sequence of stimulation is required to induce synthesis of the antimicrobial molecule nitric oxide by mouse macrophages.
Nitric oxide production by macrophages required either simultaneous or sequential exposure to gamma interferon and lipopolysaccharide; exposure to lipopolysaccharide followed by exposure to gamma interferon gave little response. The apparently evanescent nature of the lipopolysaccharide signal, necessitating persistent stimulation, could be essential to down-regulating nitric oxide production after bacteria are cleared in vivo.
Lorsbach
R B
RB
Wilkinson Laboratory of the Cancer Center, University of Kansas Medical Center, Kansas City 66160.
Russell
S W
SW
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D007371:Interferon-gamma