The effect of exposure, storage times, and types of holding media on the drag-swab monitoring technique for Salmonella.
Four maintenance media were compared for the preservation of the sensitivity of drag swabs and to assess the survival of Salmonella spp. on drag swabs at reduced temperatures. The effects of Difco double-strength skim milk (2 x SM), 2% buffered peptone water (BPW), a modified liquid Cary-Blair transport medium (LCB), and lactose broth holding medium were compared, as were storage periods of various lengths. The results with enzyme-linked immunosorbent antigen capture assay and highly selective plating media detection systems showed that 2 x SM had the highest level of recovery of salmonellae after prolonged storage, both under refrigeration at 4 C for 3 days and under frozen conditions of -15 C for 2 weeks.
Opara
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Department of Microbiology, University of Maryland, College Park 20742.
Mallinson
E T
ET
Tate
C R
CR
Carr
L E
LE
Miller
R G
RG
Stewart
L
L
Kelleher
C
C
Johnston
R W
RW
Joseph
S W
SW
D000818:Animals; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D011200:Poultry; D011201:Poultry Diseases / Q000175:diagnosis*; D011309:Preservation, Biological; D012475:Salmonella / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000662:veterinary*
D003470:Culture Media