title: Human spermatozoa produce C16-platelet-activating factor.
abstract: Recent data indicate that human spermatozoa produce platelet-activating factor as determined by the rabbit platelet [3H]serotonin release bioassay. In this report, we examined by fast atom bombardment/mass spectrometry the molecular species of platelet-activating factor generated by these germ cells. Extracted spermatozoal samples that contained platelet-activating factor bioactivity underwent straight-phase high-performance liquid chromatography, and fractions which coeluted with authentic C16- and C18-platelet-activating factor standards were subjected to fast atom bombardment/mass spectrometry. Our mass spectral data indicate that human spermatozoa synthesize C16-platelet-activating factor but not C18-platelet-activating factor.
authors.affiliation: Department of Medicine, University of Washington, Seattle 98195.
authors.forename: J M
authors.initials: JM
authors.lastname: Sanwick
authors.forename: R E
authors.initials: RE
authors.lastname: Talaat
authors.forename: F B
authors.initials: FB
authors.lastname: Kuzan
authors.forename: F T
authors.initials: FT
authors.lastname: Geissler
authors.forename: E Y
authors.initials: EY
authors.lastname: Chi
authors.forename: W R
authors.initials: WR
authors.lastname: Henderson
mesh_terms: D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D010972:Platelet Activating Factor / Q000737:chemistry*; D013094:Spermatozoa / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D005227:Fatty Acids; D010972:Platelet Activating Factor