Model analysis of enzyme monolayer- and bilayer-modified electrodes: the transient response.
The transient responses of enzyme monolayer- and bilayer-modified electrodes were theoretically analyzed. The response was numerically simulated with parameters used in the foregoing paper. The transient process consists of three elementary steps: enzymatic reaction step, substrate and mediator consumption step, and charge-carrying mediator accumulation step. Contributions of the parameters to these elementary steps and the overall response were examined. Experimental observations are successfully interpreted in terms of the theoretical results.
Tatsuma
T
T
Institute of Industrial Science, University of Tokyo, Japan.
Watanabe
T
T
Okawa
Y
Y
D015374:Biosensing Techniques; D008433:Mathematics