Genomic structure of the gene for copper/zinc-superoxide dismutase in rice.
We have isolated and determined the nucleotide sequence of the structural gene (sodB) coding for one of the copper/zinc-superoxide dismutase (Cu/Zn-SOD) isozymes from rice plants (Oryza sativa L.). The sodB gene is split into 8 exons spread about 2 kb of the chromosomal DNA, with the coding sequence beginning in the 2nd and ending in the 8th. Although none of sodB introns are inserted into similar positions in Cu/Zn-SOD genes from other sources, four of six introns in the protein-coding region are located at discriminating positions within structural domains of the protein. Genomic Southern analysis indicated that cytosolic Cu/Zn-SOD is coded for by 2-3 genes in the rice genome.
Sakamoto
A
A
Department of Biochemistry, College of Agriculture, Kyoto Prefectural University, Japan.
Okumura
T
T
Ohsuga
H
H
Tanaka
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
D004247:DNA; D013482:Superoxide Dismutase