Fumarate reductase system of filarial parasite Setaria digitata.
In the cattle filarial parasite Setaria digitata the mitochondria like particles have been shown to possess NADH dependent fumarate reduction coupled with site I electron transport associated phosphorylation. This reduction is catalysed by the fumarate reductase system. The Km for fumarate is 1.47 mM and that for NADH is 0.33 mM. This activity is sensitive to rotenone, antimycin A and o-Hydroxy diphenyl. One ATP is produced for each pair of electrons transferred to fumarate. The fumarate reductase system consisting of NADH-coenzyme Q reductase, cytochrome b like component(s) and succinate dehydrogenase/fumarate reductase is thus very important and hence specific inhibitors of the system may prove useful in the effective control of filariasis.
Unnikrishnan
L S
LS
Department of Biochemistry, University of Kerala, Thiruvananthapuram, India.
Raj
R K
RK
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002417:Cattle; D002418:Cattle Diseases; D005370:Filarioidea / Q000201:enzymology* / Q000302:isolation & purification; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D008954:Models, Biological; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology; D012719:Setariasis / Q000662:veterinary; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013385:Succinate Dehydrogenase / Q000378:metabolism*
D012402:Rotenone; D000968:Antimycin A; D013385:Succinate Dehydrogenase; D016660:NAD(P)H Dehydrogenase (Quinone)