Molecular cloning of chick liver HMG 2a cDNA and developmental expression of HMG 2a mRNA.
The cDNA clone encoding HMG 2a of chick liver was isolated from a lambda gt11 expression library using polyclonal antibodies. DNA sequence analysis revealed an open reading frame of 201 amino acids. Comparison of the nucleotide sequences of cDNA coding for chick liver HMG 2a with pig thymus HMG 2 and human monocytic leukemia cell HMG 2 showed 70% homology. 2.0 kb and 1.2 kb mRNAs were found in newly hatched chick liver and decreased during postnatal development of chicks.
Oka
T
T
Department of Nutritional Chemistry, School of Medicine, University of Tokushima, Japan.
Endo
Y
Y
Ito
M
M
Miyamoto
K
K
Sasakawa
T
T
Suzuki
I
I
Natori
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D006609:High Mobility Group Proteins / Q000235:genetics*; D008099:Liver / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis*; D012689:Sequence Homology, Nucleic Acid
D006609:High Mobility Group Proteins; D012333:RNA, Messenger; D004247:DNA