Characterization and chromosomal distribution of a tandemly repeated DNA sequence from the Australian sheep blowfly, Lucilia cuprina.
In the course of making a Lucilia cuprina genomic DNA library, a ladder of bands was seen in partial Sau3A digests. Complete digestion reduced this ladder to predominantly monomer units of approximately 190 bp. Nine independently isolated copies of this repeat were cloned and sequenced. Only two of these isolates are identical in sequence, the most divergent being 71% homologous. This satellite DNA occurs in all three wild-type strains tested, and, for the single case examined, in the embryonic, larval, pupal, and adult DNA. It represents approximately 3%-4% of the genome. Data obtained from in situ chromosome hybridizations indicate that this sequence is concentrated around the centromeric regions of the autosomes and over most of the sex chromosomes. Labelling is much stronger in mitotic compared with polytene chromosomes showing directly that this centromeric satellite DNA is grossly under-replicated during polytenization. This under-replication is even more pronounced on the sex chromosomes compared with the autosomes.
Perkins
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HD
Division of Biochemistry and Molecular Biology, School of Life Sciences, Australian National University, Canberra.
Bedo
D G
DG
Howells
A J
AJ
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002875:Chromosomes; D003001:Cloning, Molecular; D004247:DNA; D004175:Diptera / Q000235:genetics*; D008938:Mitosis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D004247:DNA