Detection of calbindin-D immunoreactivity in human follicular dendritic cells.
The calcium-binding protein calbindin-D (28 kD) has been analysed immunohistochemically in different human lymphoid tissues. Combined immunohistochemical staining showed that calbindin-D (28 kD) is expressed by only a proportion of dendritic cells within the light zone of germinal centres, where antigens in the form of immune complexes are trapped and presented to B lymphocytes. All other cell types including macrophages, interdigitating cells, and various lymphocyte populations were negative. The expression of calbindin-D in this functionally relevant subset of follicular dendritic cells could have a role in the regulation of proliferation and selection of memory B-cells by modulating the concentration of calcium ions. Calbindin-D may be a useful marker for analysing in situ the phases of follicular development in different physiological and pathological conditions.
Chilosi
M
M
Istituto di Anatomia Patologica, Università di Verona, Italy.
Doglioni
C
C
Pelosio
P
P
Mombello
A
A
Montagna
L
L
Benedetti
A
A
Lestani
M
M
Giovanni
P
P
Menestrina
F
F
D064026:Calbindins; D003713:Dendritic Cells / Q000737:chemistry*; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D012507:Sarcoidosis / Q000378:metabolism; D014123:Toxoplasmosis / Q000378:metabolism
D064026:Calbindins; D064030:S100 Calcium Binding Protein G