Sensitive amplified immunoenzymometric assays (IEMA) for human insulin and intact proinsulin.
Immunoenzymometric assays (IEMAs) for human insulin and intact proinsulin were developed using the amplification system developed by Johannsson et al. (Clin. Chim. Acta 148 (1985) 119-124) for the detection of the enzyme alkaline phosphatase. The detection limit of the assays was 0.8 pmol/l for proinsulin and 0.8 pmol/l for insulin whereas it was 1.8 pmol/l and 2.3 pmol/l respectively for the homologous immunoradiometric assays (IRMA). These assays are superior to immunoradiometric assays in terms of sensitivity, shelf-life of the labelled antibody and suitability for automation.
Alpha
B
B
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, UK.
Cox
L
L
Crowther
N
N
Clark
P M
PM
Hales
C N
CN
D000469:Alkaline Phosphatase; D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000097:blood* / Q000276:immunology; D011384:Proinsulin / Q000097:blood* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D007328:Insulin; D011384:Proinsulin; D000469:Alkaline Phosphatase