The ntrA gene of Agrobacterium tumefaciens: identification, cloning, and phenotype of a site-directed mutant.
A 3.6-kb EcoRI fragment containing the ntrA gene of Agrobacterium tumefaciens was cloned by using the homologous ntrA gene of Rhizobium meliloti as a probe. Construction of an ntrA mutant of A. tumefaciens by site-directed insertional mutagenesis demonstrated the requirement of the ntrA gene for nitrate utilization and C4-dicarboxylate transport but not for vir gene expression or tumorigenesis.
Wu
Z L
ZL
Department of Microbiology, University of Washington, Seattle 98195.
Charles
T C
TC
Wang
H
H
Nester
E W
EW
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016297:Mutagenesis, Site-Directed; D012808:Sigma Factor / Q000235:genetics*
D004269:DNA, Bacterial; D012808:Sigma Factor