Multiple types of cytokines constitutively produced by an established murine thymic epithelial cell line.
An untransformed murine thymic epithelial cell line (MTEC1) has been established. Without exogenous stimulation, the MTEC1 cells constitutively produced multiple types of cytokines, including IL-1, IL-6, IL-7, GM-CSF and chemotactic factor(s). Of which, IL-6, GM-CSF and chemotactic factor(s) were abundant; IL-1, moderate; and IL-7 at low level. MTEC1 cells neither produced detectable IL-3 nor TNF alpha. Thus, the MTEC1 cells may be useful not only in the evaluation of the signals required for thymic selection in vitro, but also useful in the analysis of the endogenous regulation of the autocrine cytokine production cascade.
Chen
W F
WF
Department of Immunology, Beijing Medical University, China.
Fan
W
W
Cao
L X
LX
Zhang
P X
PX
D000818:Animals; D002460:Cell Line / Q000378:metabolism*; D002630:Chemotactic Factors / Q000096:biosynthesis; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis; D007377:Interleukin-3 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D015851:Interleukin-7 / Q000096:biosynthesis; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D000093242:Interleukin-6 Inhibitors
D002630:Chemotactic Factors; D003115:Colony-Stimulating Factors; D016207:Cytokines; D007375:Interleukin-1; D007377:Interleukin-3; D015850:Interleukin-6; D015851:Interleukin-7; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors