Cloning and characterization of a human angiotensin II type 1 receptor.
A human liver cDNA library was screened using a rat type 1 angiotensin II receptor cDNA coding sequence as a probe. cDNA clones were isolated which encoded a protein of 359 amino acids that shared 94.4% and 95.3% identify to rat and bovine type 1 angiotensin II receptors, respectively. Ligand binding studies of the cloned receptor expressed in COS cells suggested that it is pharmacologically a type 1 angiotensin II receptor subtype. Electrophysiological studies of the receptor expressed in Xenopus laevis oocytes revealed that it could functionally couple to a second messenger system leading to the mobilization of intracellular stores of calcium. Southern and Northern blot analyses indicated that the cloned receptor is represented as a single copy in the human genome and is expressed in many tissues of different histogenic origin with the exception of brain, where mRNA transcripts were barely detectable.
Bergsma
D J
DJ
Department of Molecular Genetics, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406.
Ellis
C
C
Kumar
C
C
Nuthulaganti
P
P
Kersten
H
H
Elshourbagy
N
N
Griffin
E
E
Stadel
J M
JM
Aiyar
N
N
D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000235:genetics*; D001483:Base Sequence; D001667:Binding, Competitive; D015152:Blotting, Northern; D015139:Blotting, Southern; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006801:Humans; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D011945:Receptors, Angiotensin / Q000145:classification / Q000235:genetics*; D015398:Signal Transduction; D014018:Tissue Distribution
D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D002118:Calcium