title: Light-induced retinal damage in mice. Hydrogen peroxide production and superoxide dismutase activity in retina.
abstract: The oxidative mechanism in retinal damage due to exposure to intense light was investigated histochemically and biochemically. SMA mice (albino mice) of 2 to 3 months of age were exposed to intense light (1000-1400 lux). In the cyclic light-reared group (without dark adaptation), the outer and inner segments of the photoreceptor cells were damaged after 3 days of exposure, and severe outer nuclear layer damage was observed after 5 to 7 days of exposure. Hydrogen peroxide (H2O2) production in the outer nuclear layer increased with the progress of retinal damage. In the dark-reared group (dark adaptation of 16-18 hours), outer and inner segment damage was noted after 4 hours of light exposure, and severe outer nuclear layer damage was noted after 12 hours of light exposure. H2O2 production increased in the outer nuclear layer with retinal damage. Superoxide dismutase (SOD) activity did not change before the occurrence of retinal damage, and decreased by 25% after 3 days of exposure to light in the cyclic light-reared group. The decrease in total SOD activity corresponded to that of manganese-SOD (Mn-SOD). In the dark-reared group, SOD activity did not change, even after 1 day of exposure. There appears to be some relationship between retinal light damage and H2O2 production in the outer nuclear layer. Superoxide dismutase activity failed to provide protection against retinal oxidative damage due to intense light exposure.
authors.affiliation: Department of Ophthalmology, University of Tokyo School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamashita
authors.forename: K
authors.initials: K
authors.lastname: Horie
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Nagano
authors.forename: T
authors.initials: T
authors.lastname: Hirano
mesh_terms: D000818:Animals; D003623:Dark Adaptation; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008027:Light / Q000009:adverse effects*; D051379:Mice; D010786:Photoreceptor Cells / Q000473:pathology / Q000528:radiation effects; D012160:Retina / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D012164:Retinal Diseases / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase