Conformational analysis of p53 in resting and concanavalin A-stimulated mouse lymphocytes.
We report that p53 in resting and concanavalin A-stimulated Balb/c mouse lymphocytes cannot be distinguished on the basis of different reactivity with various epitope-specific monoclonal antibodies, regardless of whether the lymphocytes are stimulated with concanavalin A in the presence or absence of serum. Our results thus question the 'conformational hypothesis' put forward by Milner [Milner, J. (1991). Curr. Op. Cell Biol., 3, 282-286], according to which wild-type p53, depending on its conformational status, can act as a negative or a positive growth regulator.
Mosner
J
J
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie Universität Hamburg, Germany.
Deppert
W
W
D000818:Animals; D000911:Antibodies, Monoclonal; D002453:Cell Cycle; D003208:Concanavalin A / Q000008:administration & dosage; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D011487:Protein Conformation; D016159:Tumor Suppressor Protein p53 / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal; D016159:Tumor Suppressor Protein p53; D003208:Concanavalin A