title: Schistosoma haematobium: histochemistry of glycogen, glycogen phosphorylase a and glycogen branching enzyme in niridazole-treated females.
abstract: The body posterior to the ovary of Schistosoma haematobium females was investigated. Glycogen, glycogen phosphorylase a (EC 2.4.1.1) and glycogen branching enzyme (EC 2.4.1.18) activities were detected in the subtegumental muscle system, parenchyma and mature vitelline cells, whereas no activities were detected in the tegument and immature vitelline cells of the parasite. Administration of a single niridazole dose of 250 mg kg-1 to the pouched mouse (Saccostomus camestris) produced the following changes in S. haematobium females: a relatively rapid depletion of glycogen stores due to disruption of the absorptive surface of the parasite, and to an increase in the activity of glycogen phosphorylase a; a reduction in the phosphorylase a to phosphorylase b-conversion capacity of glycogen phosphorylase phosphatase (EC 3.1.3.17); a decrease in glycogen branching enzyme activity; and a relatively rapid degeneration of parasite cells possibly due to their loss of endogenous energy reserves.
authors.affiliation: University of Geneva, Switzerland.
authors.forename: T
authors.initials: T
authors.lastname: Moczon
authors.forename: Z
authors.initials: Z
authors.lastname: Swiderski
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000032:analysis; D000818:Animals; D005260:Female; D006003:Glycogen / Q000032:analysis / Q000378:metabolism*; D009115:Muridae; D009560:Niridazole / Q000627:therapeutic use*; D006005:Phosphorylases / Q000032:analysis; D012548:Schistosoma haematobium / Q000187:drug effects*; D012553:Schistosomiasis haematobia / Q000188:drug therapy*
chemical_list: D006003:Glycogen; D006005:Phosphorylases; D015061:1,4-alpha-Glucan Branching Enzyme; D009560:Niridazole