title: Occurrence of fatty acid epoxide hydrolases in soybean (Glycine max). Purification and characterization of the soluble form.
abstract: Epoxide hydrolases catalysing the hydration of cis-9,10-epoxystearate into threo-9,10-dihydroxystearate have been detected in soybean (Glycine max) seedlings. The major activity was found in the cytosol, a minor fraction being strongly associated with microsomes. The soluble enzyme, which was purified to apparent homogeneity by (NH4)2SO4 fractionation, hydrophobic, DEAE- and gel-filtration chromatographies, has a molecular mass of 64 kDa and a pI of 5.4.
authors.affiliation: Institut de Biologie Moléculaire des Plantes (CNRS UPR-406), Département d'Enzymologie Cellulaire et Moléculaire, Strasbourg, France.
authors.forename: E
authors.initials: E
authors.lastname: Blée
authors.forename: F
authors.initials: F
authors.lastname: Schuber
mesh_terms: D004851:Epoxide Hydrolases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D004852:Epoxy Compounds / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D007700:Kinetics; D008566:Membranes / Q000201:enzymology; D012639:Seeds / Q000201:enzymology*; D013025:Glycine max / Q000196:embryology / Q000201:enzymology*; D013267:Stilbenes / Q000494:pharmacology; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D004852:Epoxy Compounds; D005227:Fatty Acids; D013267:Stilbenes; C013690:styrene oxide; D004851:Epoxide Hydrolases; C025906:stilbene oxide