Survival characteristics of the epigastric free flap in DA-rats.
Epigastic flaps measuring approximately 6 x 4 cm with borders related to well-defined anatomical structures were isografted (Dark Agouty to Dark Agouty inbred rats) with or without anastomosing their epigastric pedicle, after various periods of ischaemia, ranging from 0 to 120 h. During the ischaemic insult the flaps were stored at 0 degrees C in a hypertonic citrate solution. The non-revascularized flaps exhibited survival percentages varying from 2 to 34% (mean, 16%) of the original flap,. independent of the storage interval. The flaps that were revascularized after ischaemia had a 100% survival when transplanted immediately after isolation and following 48 h of cold storage. Mean survival percentages of 78% after 72 h of ischaemia, 32% after 96 h, and 4% after 120 h of ischaemia were found. It is concluded that the model described in this study is suitable for free flap research, because of its delineated borders. However, when the area of flap survival is lower than 30% in revascularized flaps there is a distinct likelihood that the flaps have survived regardless of the pedicle as simple Wolfe or composite grafts. Results must therefore be interpreted with great care.
Werker
P M
PM
Department of Plastic, Reconstructive and Hand Surgery, University Hospital, Utrecht, The Netherlands.
Kon
M
M
Green
C
C
Franken
R J
RJ
Overgoor
M L
ML
D000818:Animals; D006085:Graft Survival; D006119:Groin / Q000098:blood supply; D008297:Male; D008866:Microsurgery; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D012867:Skin / Q000098:blood supply / Q000473:pathology; D013524:Surgical Flaps; D014021:Tissue Preservation; D014185:Transplantation, Isogeneic