Crystallization and preliminary X-ray diffraction studies of a protective cloned 28 kDa glutathione S-transferase from Schistosoma mansoni.
Crystals of the recombinant 28 kDa glutathione S-transferase from Schistosoma mansoni have been obtained by the hanging-drop method of vapor diffusion from ammonium sulfate solutions. The successful crystallization of this enzyme required the presence of a reducing agent and S-hexylglutathione. The crystals belong to the cubic space group P4(1)32 (or P4(3)32), with unit cell dimensions a = 122.6 A and contain one molecule in the asymmetric unit. The crystals diffract to at least 2.8 A resolution and are suitable for X-ray crystallographic structure analysis.
Trottein
F
F
Centre d'Immunologie des Maladies Transmissibles et Allergiques, Institut Pasteur, Lille, France.
Vaney
M C
MC
Bachet
B
B
Pierce
R J
RJ
Colloc'h
N
N
Lecocq
J P
JP
Capron
A
A
Mornon
J P
JP
D000818:Animals; D003001:Cloning, Molecular; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D012550:Schistosoma mansoni / Q000201:enzymology*; D014961:X-Ray Diffraction
D005982:Glutathione Transferase