title: Reconstituted basement membrane influences prolactin, LH, and FSH secretion from adult and fetal adenohypophyseal cells in vitro.
abstract: These investigations tested the hypothesis that secretion of prolactin (PRL), LH, and FSH in vitro is influenced by the substratum on which adult or fetal adenohypophyseal cells are cultured. Adenohypophyses were removed from adult male Golden Syrian hamsters and from fetal hamsters on day 16 of gestation. The glands were dissociated and cultured in a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 medium containing 10% fetal bovine serum (FBS), 25 mM Hepes, and antibiotics. The cells were cultured on three substrata: glass, laminin, and the reconstituted basement membrane of the Engelbreth-Holm-Swarm (EHS) tumor (Matrigel). Medium was collected and replaced every 48 h for 14-22 days. Concentrations of PRL, LH, and FSH in medium were measured by RIA. The substratum influenced hormone secretion. PRL concentrations were elevated in cultures of adult cells on Matrigel in each of four experiments. Adenohypophyseal cells on Matrigel maintained a rounded shape longer than cells on glass or laminin. In studies using fetal adenohypophyseal cells, PRL concentrations were elevated significantly in medium from cultures on Matrigel at and after 2 days as were concentrations of LH and FSH after 6 days. Additional experiments showed that the higher PRL concentrations in medium surrounding adult cells plated on Matrigel were not due to the release of soluble factors from Matrigel, differential cell attachment on Matrigel, the differential presence of adenohypophyseal fibroblasts, nor differential rates of cell proliferation. The results show that Matrigel maintains the secretion of PRL from adult adenohypophyseal cells in vitro more effectively than glass or laminin substrata and support the hypothesis that cell-matrix interactions mediate the observed differences. The results also show that in long-term cultures (14-22 days), fetal adenohypophyseal cells secrete significantly more PRL, LH, and FSH on Matrigel than they secrete when cultured on glass or laminin. Thus, Matrigel influences the function and possibly the maturation of adenohypophyseal cells in vitro. Furthermore, although laminin is the most abundant component in Matrigel, the effects of Matrigel on lactotrophs and gonadotrophs in vitro are probably not attributable solely to its laminin content.
authors.affiliation: Department of Anatomy, Cell Biology, and Neurosciences, University of South Carolina School of Medicine, Columbia 29208.
authors.forename: M J
authors.initials: MJ
authors.lastname: Horacek
authors.forename: M O
authors.initials: MO
authors.lastname: Dada
authors.forename: L
authors.initials: L
authors.lastname: Terracio
mesh_terms: D000367:Age Factors; D000818:Animals; D001485:Basement Membrane / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen; D006224:Cricetinae; D003470:Culture Media / Q000737:chemistry; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000502:physiology; D005260:Female; D005333:Fetus / Q000378:metabolism; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D005640:Follicle Stimulating Hormone / Q000032:analysis / Q000378:metabolism*; D005898:Glass; D007150:Immunohistochemistry; D007797:Laminin; D007986:Luteinizing Hormone / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D011388:Prolactin / Q000032:analysis / Q000378:metabolism*; D011509:Proteoglycans; D011863:Radioimmunoassay
chemical_list: D003470:Culture Media; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D003094:Collagen