[Typing by isoelectric focusing of beta-lactamase enzymes in Klebsiella and Enterobacter strains resistant to the new beta-lactam antibiotics].
Resistance to new generation beta-lactam antibiotics in Gram-negative bacteria is increasing worldwide. This resistance is due to EBS enzymes in Klebsiella spp., and Type-I chromosomal enzymes in Enterobacter spp., Citrobacter, P. aeruginosa, Providencia, M. morganii and S. marcescens. In this study, the types of beta-lactamases in 16 Klebsiella spp. and 15 Enterobacter spp. which are resistant to newer beta-lactam antibiotics was investigated by isoelectric focusing. The results of the study showed that TEM-1 enzymes are prevalent in these isolates. pI (isoelectric point) values and antibiotic susceptibility test results suggest that there are EBS enzymes in Klebsiella spp., and Type-I enzymes in Enterobacter spp. These results have to be supported by further investigations.
Gür
D
D
Hacettepe Universitesi Iç Hastaliklari Anabilim Dali Infeksiyon Unitesi.
Akalin
H E
HE
Baykal
M
M
Doğrul
F
F
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004754:Enterobacter / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D007709:Klebsiella / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000032:analysis*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases
Yeni kuşak beta-laktam antibiyotiklere dirençli Klebsiella ve Enterobacter suşlarinda "Isoelectric Focusing" yöntemi kullanilarak beta-laktamaz enzimlerinin tiplendirilmesi.