The Drosophila per gene homologs are expressed in mammalian suprachiasmatic nucleus and heart as well as in molluscan eyes.
This study presents evidence for the conservation of Drosophila per gene homologs in mammalian DNA and for their expression in a number of tissues which are involved in various aspects of circadian timekeeping. Distinct 5 kb sequences, which hybridized to a non repetitive fragment of the Drosophila per gene under stringent conditions, were detected by Southern blotting. Sequences homologous to per gene of Drosophila were also amplified from rat and mouse brain cDNA libraries and from a mouse anterior hypothalamus and human hypothalamus libraries. Degenerate PCR primer design was based on conserved segments of the per protein. The per homologs were shown directly (by RT-PCR) to be expressed in hamster and mouse SCN, in hamster heart and in Aplysia and Bulla eyes.
Maler
T
T
Department of Psychiatry, Toronto Western Hospital, Canada.
Ralph
M R
MR
Gorczynski
R M
RM
Moldofsky
H
H
O'Dowd
B F
BF
Du
D C
DC
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D016466:CHO Cells; D006224:Cricetinae; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004330:Drosophila / Q000235:genetics*; D015723:Gene Library; D005796:Genes; D006321:Heart / Q000502:physiology*; D008322:Mammals; D008647:Mesocricetus; D051379:Mice; D008969:Molecular Sequence Data; D008974:Mollusca / Q000235:genetics*; D009799:Ocular Physiological Phenomena; D016133:Polymerase Chain Reaction; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013493:Suprachiasmatic Nucleus / Q000502:physiology*
D004247:DNA