title: Determination of plasma [6,6-2H2]glucose enrichment by a simple and accurate gas chromatographic-mass spectrometric method.
abstract: An improved method for the evaluation of glucose turnover rate in humans, using a prime-continuous infusion of [6,6-2H2]glucose, was developed. Deproteinization of plasma and conversion of glucose into the aldononitrile pentaacetate derivative are the only sample manipulations required prior to the gas chromatographic-mass spectrometric analysis. In six normal adults (prime = 5 mg kg-1; continuous infusion = 0.05 mg kg-1 min-1) the hepatic glucose output calculated at steady state by the procedure described here was 2.1 +/- 0.2 mg kg-1 min-1, the isotopic enrichment being determined with a coefficient of variation of ca. 2%. In six additional subjects, with half of the above-mentioned doses of labelled glucose, the mean hepatic glucose output was exactly the same (3.2% coefficient of variation for the isotopic enrichment measurement). The method described allows the hepatic glucose output to be precisely determined with savings both of time and of labelled glucose.
authors.affiliation: Department of Medicine, Scientific Institute S. Raffaele, Milan, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Magni
authors.forename: L
authors.initials: L
authors.lastname: Monti
authors.forename: P
authors.initials: P
authors.lastname: Brambilla
authors.forename: R
authors.initials: R
authors.lastname: Poma
authors.forename: G
authors.initials: G
authors.lastname: Pozza
authors.forename: M
authors.initials: M
authors.lastname: Galli Kienle
mesh_terms: D001786:Blood Glucose / Q000032:analysis*; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D003903:Deuterium; D005947:Glucose