Suitability of mitochondrial DNA for assaying interindividual genetic variation in small helminths.
The current paucity of data on the genetic structure of parasitic helminth populations results partly from the lack of a suitable molecular technique for assigning genotypes to small individuals. This report describes the cloning of the mitochondrial DNA (mtDNA) from a small parasitic nematode, Ostertagia ostertagi, and the potential use of this cloned mtDNA as a hybridization probe to detect genetic variation among individuals. By using cloned, homologous mtDNA, labeled to high specific activity, mtDNA restriction site variation can be assayed among individual O. ostertagi for at least 10 restriction enzymes.
Tarrant
C A
CA
Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville 32611-0633.
Blouin
M S
MS
Yowell
C A
CA
Dame
J B
JB
D000018:Abomasum / Q000469:parasitology; D000818:Animals; D001345:Autoradiography; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D002499:Centrifugation, Density Gradient; D003001:Cloning, Molecular; D015342:DNA Probes / Q000032:analysis / Q000302:isolation & purification; D004272:DNA, Mitochondrial / Q000032:analysis / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI; D005260:Female; D014644:Genetic Variation; D009693:Nucleic Acid Hybridization; D010028:Ostertagia / Q000235:genetics*; D010029:Ostertagiasis / Q000469:parasitology / Q000662:veterinary; D015183:Restriction Mapping
D015342:DNA Probes; D004272:DNA, Mitochondrial; D015246:Deoxyribonuclease EcoRI