title: Isolation of monochromosomal hybrids following fusion of human diploid fibroblast-derived microcells with mouse A9 cells.
abstract: Rodent x human hybrids containing single human chromosomes are valuable tools in the study of human genetics. Using a retrovirus carrying the neomycin gene, we generated a neomycin-resistant (neor) pool of human diploid fibroblasts. Following fusion of microcells prepared from this pool to mouse A9 cells, we obtained four neor hybrids containing a single human chromosome 2, 3, 5, or 15.
authors.affiliation: Roy M. and Phyllis Gough Huffington Center on Aging, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Y
authors.initials: Y
authors.lastname: Ning
authors.forename: M
authors.initials: M
authors.lastname: Lovell
authors.forename: L
authors.initials: L
authors.lastname: Taylor
authors.forename: O M
authors.initials: OM
authors.lastname: Pereira-Smith
mesh_terms: D000818:Animals; D002459:Cell Fusion / Q000235:genetics*; D002460:Cell Line; D002877:Chromosomes, Human; D002884:Chromosomes, Human, Pair 15; D002889:Chromosomes, Human, Pair 2; D002893:Chromosomes, Human, Pair 3; D002895:Chromosomes, Human, Pair 5; D004351:Drug Resistance / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D009355:Neomycin / Q000494:pharmacology
chemical_list: D009355:Neomycin