title: Protein disulfide isomerase activity is released by activated platelets.
abstract: The release of protein disulfide isomerase by activated platelets was hypothesized on the basis of reported intermolecular and intramolecular thiol-disulfide exchange and disulfide reduction involving released thrombospondin in the supernatant solution of activated platelets (Danishefsky, Alexander, Detwiler: Biochemistry, 23:4984, 1984; Speziale, Detwiler: J Biol Chem, 265:17859, 1990; Speziale, Detwiler: Arch Biochem Biophys 286:546, 1991). Protein disulfide isomerase activity, measured by catalysis of the renaturation of ribonuclease inactivated by randomization of disulfide bonds, was detected in the supernatant solution after platelet activation. The activity was inhibited by peptides known to inhibit protein disulfide isomerase; the peptides also inhibited formation of disulfide-linked thrombospondin-thrombin complexes. The reaction catalyzed by the supernatant solution showed a pH dependence distinct from that of the uncatalyzed reaction. The activity was excluded by a 50-Kd dialysis membrane, and it was eluted in the void volume of a gel-filtration column, indicating that it was associated with a macromolecule. The activity was not removed by centrifugation at 100,000 g for 150 minutes indicating that it was not associated with membrane microvesicles. Possible functions for the release of protein disulfide isomerase by activated platelets are discussed.
authors.affiliation: Department of Biochemistry, State University of New York Health Science Center, Brooklyn 11203.
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Lin
authors.forename: T C
authors.initials: TC
authors.lastname: Detwiler
mesh_terms: D001792:Blood Platelets / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D007535:Isomerases / Q000378:metabolism*; D015539:Platelet Activation; D019704:Protein Disulfide-Isomerases
chemical_list: D007535:Isomerases; D019704:Protein Disulfide-Isomerases