title: An animal model of Kaposi's sarcoma. I. Immune status of CD1 mice undergoing dimethyl hydrazine treatment to induce angiosarcomas and other malignancies.
abstract: Dimethylhydrazine (DMH) induces colonic cancer and angiosarcomas in mice. In order to determine pertinence of mouse angiosarcoma as a model to AIDS associated Kaposi's sarcoma (KS), we investigated if immune dysfunction occurred during tumor development by DMH. Outbred CD1 male mice received once weekly DMH a 20 mg/kg body weight dose s.c. for 33 weeks. Every two weeks initially and then every week groups of DMH-treated and control animals were sacrificed to determine a) peripheral blood and splenic T cell subset ratio b) 4-day plaque forming cell (PFC) response to i.p. sheep red blood cells (SRBC) and c) mitogenic response of spleen cells to Concanavalin A (Con A) and lipopolysaccharide (LPS). No change in T helper/T suppressor + cytotoxic T cell (Th/Tsupp. + CTL) and mitogenic response to spleen cells to Con A was noted whereas PFC response of animals to SRBC and mitogenic response of spleen cells to LPS decreased. These data suggest that either infection with T cell depleting virus such as LP:BM5 or immunosuppressive drugs affecting T cell function, such as steroids may be required to bring the immune status of DMH treated animals closer to that of AIDS associated KS bearing human subjects.
authors.affiliation: Departement d'Anatomie et Biologie Cellulaire, Faculté de Médécine, Université de Sherbrooke, Québec, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Dubé
authors.forename: P
authors.initials: P
authors.lastname: Madarnas
authors.forename: M
authors.initials: M
authors.lastname: Rola-Pleszczynski
authors.forename: V N
authors.initials: VN
authors.lastname: Nigam
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004127:Dimethylhydrazines; D004195:Disease Models, Animal; D000518:Eflornithine / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D006394:Hemangiosarcoma / Q000139:chemically induced / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D012514:Sarcoma, Kaposi / Q000209:etiology* / Q000276:immunology; D012756:Sheep; D013154:Spleen / Q000276:immunology / Q000473:pathology
chemical_list: D004127:Dimethylhydrazines; D000518:Eflornithine