Linear correlation between acetoacetate/beta-hydroxybutyrate in arterial blood and oxidized flavoprotein/reduced pyridine nucleotide in freeze-trapped human liver tissue.
A comparison of two methods of measuring liver mitochondrial redox state demonstrated that a linear correlation exists between acetoacetate/beta-hydroxybutyrate ratio in arterial blood (arterial ketone body ratio; AKBR) and oxidized flavoprotein/reduced pyridine nucleotide in human liver tissue (FP/PN) as measured by tissue fluorescence spectroscopy, such that [FP/PN] = 0.64 + 0.49 x [AKBR] (r = 0.84, P less than 0.001). This result supports the validity of AKBR as a method of measuring the hepatic mitochondrial redox state of pyridine nucleotide using arterial blood.
Ozawa
K
K
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Chance
B
B
Tanaka
A
A
Iwata
S
S
Kitai
T
T
Ikai
I
I
D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000097:blood*; D002087:Butyrates / Q000097:blood*; D005420:Flavoproteins / Q000097:blood; D005453:Fluorescence; D005615:Freezing; D006801:Humans; D006885:Hydroxybutyrates / Q000097:blood*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000097:blood; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity
D000090:Acetoacetates; D002087:Butyrates; D005420:Flavoproteins; D006885:Hydroxybutyrates; D009243:NAD; C016635:acetoacetic acid; D020155:3-Hydroxybutyric Acid