title: Crystallization and preliminary X-ray diffraction studies of a protective cloned 28 kDa glutathione S-transferase from Schistosoma mansoni.
abstract: Crystals of the recombinant 28 kDa glutathione S-transferase from Schistosoma mansoni have been obtained by the hanging-drop method of vapor diffusion from ammonium sulfate solutions. The successful crystallization of this enzyme required the presence of a reducing agent and S-hexylglutathione. The crystals belong to the cubic space group P4(1)32 (or P4(3)32), with unit cell dimensions a = 122.6 A and contain one molecule in the asymmetric unit. The crystals diffract to at least 2.8 A resolution and are suitable for X-ray crystallographic structure analysis.
authors.affiliation: Centre d'Immunologie des Maladies Transmissibles et Allergiques, Institut Pasteur, Lille, France.
authors.forename: F
authors.initials: F
authors.lastname: Trottein
authors.forename: M C
authors.initials: MC
authors.lastname: Vaney
authors.forename: B
authors.initials: B
authors.lastname: Bachet
authors.forename: R J
authors.initials: RJ
authors.lastname: Pierce
authors.forename: N
authors.initials: N
authors.lastname: Colloc'h
authors.forename: J P
authors.initials: JP
authors.lastname: Lecocq
authors.forename: A
authors.initials: A
authors.lastname: Capron
authors.forename: J P
authors.initials: JP
authors.lastname: Mornon
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D012550:Schistosoma mansoni / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D005982:Glutathione Transferase