title: In situ localization of transforming growth factor beta and S-phase cells in patients with acute myeloid leukemia and myelodysplastic syndrome.
abstract: In situ localization of transforming growth factor beta (TGF-B) was accomplished in 16 acute myeloid leukemia (AML) and 11 myelodysplastic syndrome (MDS) cases. A modified and greatly shortened procedure for immunohistochemical localization of TGF-B using a monoclonal antibody was undertaken in plastic embedded bone marrow biopsy sections that demonstrated excellent preservation of morphologic detail. In addition, a double-label procedure was developed for the simultaneous detection of TGF-B and cells actively engaged in DNA-synthesis which were labeled in vivo by infusing thymidine analogues. Marked variation in intensity of TGF-B staining in interstitial areas of biopsies was noted among different patients. TGF-B was also conspicuously present in the basement membrane of blood vessel endothelial cells and the cytoplasm of megakaryocytes. Double-labeling revealed that S-phase cells in a number of MDS cases appeared clustered together and that megakaryocytes in S-phase were completely devoid of TGF-B.
authors.affiliation: Department of Internal Medicine, University of Cincinnati, Ohio 45267-0508.
authors.forename: K
authors.initials: K
authors.lastname: Qadir
authors.forename: S A
authors.initials: SA
authors.lastname: Bokhari
authors.forename: M A
authors.initials: MA
authors.lastname: Miller
authors.forename: C
authors.initials: C
authors.lastname: Siegrist
authors.forename: J
authors.initials: J
authors.lastname: Bismayer
authors.forename: A
authors.initials: A
authors.lastname: Raza
mesh_terms: D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism / Q000473:pathology*; D009190:Myelodysplastic Syndromes / Q000378:metabolism / Q000473:pathology*; D016196:S Phase; D016212:Transforming Growth Factor beta / Q000032:analysis*
chemical_list: D016212:Transforming Growth Factor beta