A novel calcitonin-encoding mRNA is produced by alternative processing of calcitonin/calcitonin gene-related peptide-I pre-mRNA.
The calcitonin/calcitonin gene-related peptide-I (CALC-I) gene was one of the first examples of a cellular gene exhibiting alternative, tissue-specific processing of its primary RNA transcript. Calcitonin (CT) mRNA is the predominant product in thyroid C cells, whereas calcitonin gene-related peptide-I mRNA is the main product in neurons of the central and peripheral nervous systems. In this study, we report the existence of a novel CT-like (CT*) mRNA derived from the human CALC-I gene. CT* mRNA is the result of a novel splicing reaction which was initially detected in vitro. Translation of CT* mRNA will result in a prohormone (proCT*) which only differs from proCalcitonin (proCT) in its carboxyl terminus. Proteolytic processing of proCT* predicts, beside the amino-terminal cleavage peptide and CT, the existence of a novel carboxyl-terminal protein of 21 amino acids.
Adema
G J
GJ
Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, The Netherlands.
Baas
P D
PD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002116:Calcitonin / Q000235:genetics*; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D012322:RNA Precursors; D012333:RNA, Messenger; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide