Characterization of a 7-kilodalton subunit of vaccinia virus DNA-dependent RNA polymerase with structural similarities to the smallest subunit of eukaryotic RNA polymerase II.
A previously unrecognized 7-kDa polypeptide copurified with the DNA-dependent RNA polymerase of vaccinia virus virions. Internal amino acid sequences of the small protein matched a viral genomic open reading frame of 63 codons. Antipeptide antiserum was used to confirm the specific and complete association of the 7-kDa protein with RNA polymerase. The amino acid sequence predicted from the viral gene, named rpo7, was 23% identical to that of the smallest subunit of Saccharomyces cerevisiae RNA polymerase II, and a metal-binding motif, Cys-X-X-Cys-Gly, was located at precisely the same location near the N terminus in the two proteins. RNA analyses demonstrated early transcriptional initiation and termination signals in the rpo7 gene sequence. The viral RNA polymerase subunit was synthesized during the early phase of infection and continued to accumulate during the late phase.
Amegadzie
B Y
BY
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Ahn
B Y
BY
Moss
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D016384:Consensus Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D046911:Macromolecular Substances; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012319:RNA Polymerase II / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014616:Vaccinia virus / Q000201:enzymology*; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification
D046911:Macromolecular Substances; D008670:Metals; D012333:RNA, Messenger; D014764:Viral Proteins; C074060:rpo7 protein, Vaccinia virus; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases