title: Small bowel tissue high-energy phosphate alterations during hypothermic storage using different protecting solutions.
abstract: Since posttransplant function is closely related to energy status after the storage period, the aim of our study was to determine high-energy phosphates in rat small bowels after 21 h of simple ischemic cold storage using different storage solutions. We compared Eurocollins-solution, Bretschneiders-HTK-solution and a modified University of Wisconsin-solution. After flushing the small bowel both intravascularly and intraluminally with the protecting solution, segments of rat small bowel were stored in the same solution at 4 degrees C for 21 h. Determination of high-energy phosphates by high-performance liquid chromatography were done at indicated times. We found that small bowel stored in Eurocollins-solution produced the best graft viability after 21 h of cold storage by maintaining a sufficient level of high-energy phosphates. The high ATP levels were produced by the pronounced anaerobic glycolysis which was facilitated by the high concentration of glucose in the Eurocollins-solution.
authors.affiliation: Department of Infectious Diseases, University of Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Burgmann
authors.forename: H
authors.initials: H
authors.lastname: Reckendorfer
authors.forename: M
authors.initials: M
authors.lastname: Sperlich
authors.forename: P G
authors.initials: PG
authors.lastname: Spieckermann
mesh_terms: D000241:Adenosine; D000244:Adenosine Diphosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis*; D000493:Allopurinol; D000818:Animals; D015925:Cryopreservation; D005947:Glucose; D005978:Glutathione; D006982:Hypertonic Solutions; D007328:Insulin; D007421:Intestine, Small; D008297:Male; D008353:Mannitol; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011189:Potassium Chloride; D011343:Procaine; D011887:Raffinose; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions
chemical_list: C039984:Bretschneider cardioplegic solution; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D011343:Procaine; D000244:Adenosine Diphosphate; D000493:Allopurinol; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose