title: Experimental evidence in favor of role of intracellular actions of bupivacaine in myocardial depression.
abstract: Bupivacaine is more cardiodepressant than lidocaine. Nevertheless, the marked depression of contractility induced by bupivacaine cannot be completely explained by its electrophysiologic properties alone. Biophysical differences such as the greater lipid solubility of bupivacaine versus lidocaine must be taken into consideration. Perhaps more bupivacaine enters the cardiac cells and interacts with contractile processes. To test this hypothesis, the entry of lidocaine into the cells was facilitated by a membrane-permeant lipophilic anion, tetraphenylboron. We compared the spontaneous atrial rate and the contractile force of rabbit right atria bathing in solutions containing either 0.5 microgram/mL lidocaine or bupivacaine. Group 1 (n = 8) served to test the stability of the preparation. In group 2 (n = 6), tetraphenylboron (17 micrograms/mL) was added to Tyrode's solution; atrial rate was decreased by 8% and contractile force by 1.7%. In group 3 (n = 6), bupivacaine (0.5 microgram/mL) was added; bupivacaine decreased atrial rate by 11.3% and markedly depressed contractile force by 68.3%. In group 4 (n = 6), lidocaine (0.5 microgram/mL) was added; lidocaine did not change atrial rate but decreased contractile force by 6.0%. In group 5 (n = 6), both lidocaine and tetraphenylboron were added; atrial rate was decreased by 15.5% and contractile force was markedly depressed by 81.1%. In group 6 (n = 6), 0.2 mM adenosine triphosphate, tetraphenylboron, and then lidocaine were added; the addition of adenosine triphosphate partially counteracted the cardiodepressant effects of the combination of lidocaine and tetraphenylboron. Atrial rate was decreased by 10.4% and contractile force was depressed by 13.6%.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratory of Cardiovascular Physiology, Montpellier Medical School, France.
authors.forename: J E
authors.initials: JE
authors.lastname: de La Coussaye
authors.forename: B
authors.initials: B
authors.lastname: Bassoul
authors.forename: B
authors.initials: B
authors.lastname: Albat
authors.forename: P A
authors.initials: PA
authors.lastname: Peray
authors.forename: J P
authors.initials: JP
authors.lastname: Gagnol
authors.forename: J J
authors.initials: JJ
authors.lastname: Eledjam
authors.forename: A
authors.initials: A
authors.lastname: Sassine
mesh_terms: D000818:Animals; D002045:Bupivacaine / Q000494:pharmacology*; D003864:Depression, Chemical; D004347:Drug Interactions; D006321:Heart / Q000187:drug effects*; D008012:Lidocaine / Q000494:pharmacology; D009200:Myocardial Contraction / Q000187:drug effects*; D011817:Rabbits; D013775:Tetraphenylborate / Q000494:pharmacology
chemical_list: D013775:Tetraphenylborate; D008012:Lidocaine; D002045:Bupivacaine