title: Plant transformation: a simple particle bombardment device based on flowing helium.
abstract: We observed that flowing helium at moderate pressures accelerated DNA-coated microprojectiles to velocities suitable for penetration of cells in intact plant tissues. The flowing helium principle permitted the construction of a simple and inexpensive transformation device that was easier to use than those previously described. This device provided efficient transformation of cells in soybean seedlings and other plants.
authors.affiliation: Department of Plant Pathology, University of California, Riverside 92521.
authors.forename: Y
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: M
authors.initials: M
authors.lastname: Dotson
authors.forename: N T
authors.initials: NT
authors.lastname: Keen
mesh_terms: D004864:Equipment and Supplies; D005821:Genetic Techniques; D006371:Helium; D013025:Glycine max / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D006371:Helium