Plant transformation: a simple particle bombardment device based on flowing helium.
We observed that flowing helium at moderate pressures accelerated DNA-coated microprojectiles to velocities suitable for penetration of cells in intact plant tissues. The flowing helium principle permitted the construction of a simple and inexpensive transformation device that was easier to use than those previously described. This device provided efficient transformation of cells in soybean seedlings and other plants.
Takeuchi
Y
Y
Department of Plant Pathology, University of California, Riverside 92521.
Dotson
M
M
Keen
N T
NT
D004864:Equipment and Supplies; D005821:Genetic Techniques; D006371:Helium; D013025:Glycine max / Q000235:genetics*; D014170:Transformation, Genetic
D006371:Helium