title: Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R.
abstract: The hemagglutinin of influenza virus A/FPV/Rostock/34 (H7) was altered at its multibasic cleavage site by site-directed mutagenesis and assayed for proteolytic activation after expression in CV-1 cells. The results indicated that the cellular protease responsible for activation recognizes the tetrapeptide motif R-X-K/R-R that must be presented in the correct sequence position. Studies on plaque variants of influenza virus A/fowl/Victoria/75 (H7N7) showed that alteration of the consensus sequence resulted in a loss of pathogenicity for chickens.
authors.affiliation: Institut für Virologie, Philipps-Universität Marburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Vey
authors.forename: M
authors.initials: M
authors.lastname: Orlich
authors.forename: S
authors.initials: S
authors.lastname: Adler
authors.forename: H D
authors.initials: HD
authors.lastname: Klenk
authors.forename: R
authors.initials: R
authors.lastname: Rott
authors.forename: W
authors.initials: W
authors.lastname: Garten
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D010450:Endopeptidases / Q000378:metabolism*; D006389:Hemagglutinins, Viral / Q000378:metabolism*; D009980:Influenza A virus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011392:Proline / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D011392:Proline; D010450:Endopeptidases