title: Effect of UVC light on growth, incorporation of thymidine, and DNA chain elongation in cells derived from the Indian meal moth and the cabbage looper.
abstract: After exposure to 10 or 20 J/m2 UVC light, cells of the UMN-PIE-1181 line, an embryonic cell line derived from the Indian meal moth, Plodia interpunctella, exhibited a rapid and prolonged depression in the rate of incorporation of [3H]thymidine, whereas cells of the TN-368 line, an ovarian cell line derived from Trichoplusia ni, the cabbage looper, showed only a slight drop in incorporation and a rapid recovery after exposure to 10 or 40 J/m2 UVC light. The extent of this depression was not correlated to the amount of cell killing by UVC light in these cell lines or in IAL-PID2 cells. Blockage of fork progression was correlated to the depression in thymidine incorporation. TN-368 cells exhibited little blockage after exposure to 10 or 20 J/m2 UVC light, whereas UMN-PIE-1181 cells exhibited significant blockage at these fluences. Photoreactivation did not entirely relieve blockage, depression in thymidine incorporation, or cell killing, indicating that, although the (5-6) dimer appears to be the major lesion responsible for these effects, other lesions such as the (6-4) photoproduct may play a role.
authors.affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb 60115.
authors.forename: S C
authors.initials: SC
authors.lastname: Styer
authors.forename: T D
authors.initials: TD
authors.lastname: Griffiths
mesh_terms: D000818:Animals; D002455:Cell Division / Q000528:radiation effects*; D002460:Cell Line; D004247:DNA / Q000528:radiation effects*; D004261:DNA Replication / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D008027:Light; D009036:Moths / Q000166:cytology / Q000528:radiation effects*; D013936:Thymidine / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D004247:DNA; D013936:Thymidine