title: Electron microscopy of native cartilage oligomeric matrix protein purified from the Swarm rat chondrosarcoma reveals a five-armed structure.
abstract: Cartilage oligomeric matrix protein was isolated in the native state from the Swarm rat chondrosarcoma. A crucial step was its selective extraction with EDTA-containing buffer. The purified protein was subjected to electron microscopy using rotary shadowing and negative stain. The images allowed the construction of a structural model. The bouquet-like protein consists of five 28-nm-long arms containing a peripheral globular domain, a flexible strand, and a central assembly domain, where the five arms meet in a cylindrical structure.
authors.affiliation: Department of Biophysical Chemistry, University of Basel, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Mörgelin
authors.forename: D
authors.initials: D
authors.lastname: Heinegård
authors.forename: J
authors.initials: J
authors.lastname: Engel
authors.forename: M
authors.initials: M
authors.lastname: Paulsson
mesh_terms: D000818:Animals; D002813:Chondrosarcoma / Q000737:chemistry*; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000302:isolation & purification / Q000648:ultrastructure*; D046911:Macromolecular Substances; D064235:Matrilin Proteins; D008854:Microscopy, Electron; D008961:Models, Structural; D008970:Molecular Weight; D011487:Protein Conformation; D051381:Rats; D012513:Sarcoma, Experimental / Q000737:chemistry*
chemical_list: D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D046911:Macromolecular Substances; D064235:Matrilin Proteins; D004492:Edetic Acid