A potential animal model for studying CF heterozygote advantage: genetic variation in theophylline-inducible colonic chloride currents among inbred strains of mice.
We have used Ussing chambers to measure chloride secretion by colonic segments (mucosa, muscularis, and serosa) from various inbred strains of mice. We found lower theophylline-induced Cl- secretion in the DBA/2J than in the C57BL/6J strain. Their F1 showed significantly higher levels of Cl- secretion than did the C57BL/6J parental strain while colonic segments from five recombinant inbred B x D lines ranged between the C57BL/6J and F1 values. No major component of the variation appeared to be associated with alleles of the met oncogene region of chromosome 6 or the H-2 region of chromosome 17.
Sweet
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A
Department of Human Genetics, University of Michigan School of Medicine, Ann Arbor 48109.
Erickson
R P
RP
Huntington
C
C
Dawson
D
D
D000818:Animals; D002712:Chlorides / Q000378:metabolism*; D003106:Colon / Q000187:drug effects / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000235:genetics*; D004195:Disease Models, Animal; D004553:Electric Conductivity; D005260:Female; D014644:Genetic Variation; D006579:Heterozygote; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019859:Proto-Oncogene Proteins c-met; D013806:Theophylline / Q000494:pharmacology
D002712:Chlorides; D011518:Proto-Oncogene Proteins; D013806:Theophylline; D019859:Proto-Oncogene Proteins c-met