Hexamethylenebisacetamide selectively inhibits the proliferation of human and rat vascular smooth-muscle cells.
Hexamethylenebisacetamide (HMBA) selectively and reversibly inhibited proliferation of human and rat vascular smooth-muscle cells (VSMCs) compared with endothelial cells, fibroblasts or lymphocytes. Half-maximal inhibition of VSMC proliferation occurred at 2-5 mM-HMBA, and at 30- greater than 50 mM for other cell types. HMBA also prevented de-differentiation, defined by the loss of smooth-muscle-specific myosin heavy chain, of primary rat VSMCs and caused partial re-differentiation of subcultured cells. Other inhibitors of ADP-ribosyltransferase were also selective inhibitors of VSMC proliferation.
Grainger
D J
DJ
Department of Biochemistry, University of Cambridge, U.K.
Hesketh
T R
TR
Weissberg
P L
PL
Metcalfe
J C
JC
D000081:Acetamides / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003374:Coumarins / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006397:Hematinics / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects; D011799:Quinazolines / Q000494:pharmacology; D052999:Quinazolinones; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D000081:Acetamides; D003374:Coumarins; D006397:Hematinics; D011799:Quinazolines; D052999:Quinazolinones; C061339:4-hydroxyquinazoline; C030123:coumarin; C014026:hexamethylene bisacetamide; D013936:Thymidine