Assignment of casein kinase 2 alpha sequences to two different human chromosomes.
Human casein kinase 2 alpha gene (CK-2-alpha) sequences have been localized within the human genome by in situ hybridization and somatic cell hybrid analysis using a CK-2 alpha cDNA as a probe. By in situ hybridization, the CK-2 alpha cDNA could be assigned to two different loci, one on 11p15.1-ter and one on 20p13. The existence of two separate chromosomal loci suggests that CK-2 alpha is a member of a gene family. Only the locus on chromosome 11 was confirmed by somatic cell hybrid analysis. The analysis was based on the presence of a CK-2-alpha-specific 20-kb fragment. However, the CK-2 alpha cDNA hybridizes to several additional fragments in total human DNA.
Boldyreff
B
B
Institut für Humangenetik, Universität des Saarlandes, Homburg, Federal Republic of Germany.
Klett
C
C
Göttert
E
E
Geurts van Kessel
A
A
Hameister
H
H
Issinger
O G
OG
D015139:Blotting, Southern; D047388:Casein Kinases; D002880:Chromosomes, Human, Pair 11; D002890:Chromosomes, Human, Pair 20; D015342:DNA Probes / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D005810:Multigene Family / Q000235:genetics; D009693:Nucleic Acid Hybridization; D011494:Protein Kinases / Q000235:genetics*
D015342:DNA Probes; D011494:Protein Kinases; D047388:Casein Kinases