Expression of major histocompatibility complex antigens and CR3 complement receptors in activated microglia following an injection of ricin into the sciatic nerve in rats.
The ventral horn motor neurons in the lower lumbar cord underwent rapid degeneration following an injection of Ricinus communis agglutinin-60 (RCA) into the sciatic nerve. The cell death which was most drastic between the fifth and seventh post-injection day elicited a significant increase in the number of microglia. The activated microglia were scattered throughout the neuropil but the dramatic feature was their close association with the somata of the degenerating neurons. Often several microglial cells were seen surrounding the soma of a degenerating neuron. Immunocytochemical study showed that both the interstitial as well as the perineuronal activated microglia were labelled with the monoclonal antibodies OX-18 and OX-42 for the detection of MHCI encoded antigen and type three complement receptors, respectively. Intense immunoreactivity was observed especially in the perineuronal microglia with OX-18. Electron microscopic study confirmed the identification of the activated microglia. Although the activated microglia closely apposed the neuronal soma, there was no sign of a direct endocytosis. The cytoplasm of the activated microglia, however, contained massive lipofuscin bodies in longer survival animals. Electron microscopic immunocytochemical study showed that the immunoreactivity of the activated microglia was localized along their plasma membrane facing the neuronal soma. Since the microglia cells on the contralateral side of the ventral horn were not marked by the antibodies used, it was postulated that the vigorous expression of MHCI antigen and CR3 receptors on the activated microglia was induced by the neuronal degeneration resulting from the application of the toxin ricin.
Ling
E A
EA
Department of Anatomy, National University of Singapore.
Kaur
C
C
Wong
W C
WC
D000818:Animals; D016923:Cell Death; D003176:Complement C3 / Q000187:drug effects; D015870:Gene Expression; D008285:Major Histocompatibility Complex / Q000187:drug effects* / Q000276:immunology; D008297:Male; D016253:Microscopy, Immunoelectron; D009457:Neuroglia / Q000187:drug effects / Q000276:immunology*; D009474:Neurons / Q000276:immunology / Q000473:pathology; D051381:Rats; D011951:Receptors, Complement / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D012276:Ricin / Q000378:metabolism*; D012584:Sciatic Nerve / Q000187:drug effects / Q000276:immunology; D013116:Spinal Cord / Q000276:immunology / Q000473:pathology
D003176:Complement C3; D011951:Receptors, Complement; D012276:Ricin