Effect of interferon-gamma priming on the activation of murine peritoneal macrophages to tumouricidal state by cisplatin, IL-1, and tumour necrosis factor (TNF): production of IL-1 and TNF.
The effect of interferon-gamma (IFN-gamma) priming of murine peritoneal macrophages on the activation to tumouricidal state by cisplatin, lipopolysaccharide (LPS)-IL-1 and TNF was investigated. Cisplatin-, LPS-, IL-1- or TNF-treated IFN-gamma-primed macrophages showed significantly enhanced tumouricidal activity and binding to tumour cells, compared with unprimed treated or untreated macrophages. Macrophages treated with cisplatin, LPS, IL-1 and TNF produced released and membrane-associated IL-1 and TNF activity which was significantly enhanced after priming with IFN-gamma. These observations suggest the use of IFN-gamma along with these biological response modifiers in designing immunotherapeutic protocols for treatment of malignancy.
Sodhi
A
A
School of Biotechnology, Banaras Hindu University, Varanasi, India.
Singh
R K
RK
Singh
S M
SM
D000818:Animals; D002945:Cisplatin / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000502:physiology; D005260:Female; D007371:Interferon-gamma / Q000502:physiology*; D007375:Interleukin-1 / Q000096:biosynthesis / Q000494:pharmacology; D008070:Lipopolysaccharides; D008262:Macrophage Activation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D015846:Monokines / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D014407:Tumor Cells, Cultured / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000494:pharmacology
D007375:Interleukin-1; D008070:Lipopolysaccharides; D015846:Monokines; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D002945:Cisplatin