Application of quantitative immunofluorescence to clinical serology: antibody levels of Treponema pallidum.
A previously reported method of quantitative immunofluorescence, employing a calibrated photometric system and chemically stabilized fluorescence intensity, was used to replace the subjective, visual method of endpoint determination with a quantitative, calibrated measurement of antibodies to Treponema pallidum in serum. The results of the quantitative immunofluorescence method showed a 90% correlation with the subjective determinations of the visual method.
Picciolo
G L
GL
Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland 20852.
Kaplan
D S
DS
D000907:Antibodies, Bacterial / Q000032:analysis*; D004226:Dithioerythritol / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006801:Humans; D014210:Treponema pallidum / Q000276:immunology*
D000907:Antibodies, Bacterial; D004226:Dithioerythritol