Enzymatic in vitro synthesis of radiolabeled pentasaccharides GlcNAc beta 1-3(Gal beta 1-4GlcNAc beta 1-6)Gal beta 1-4GlcNAc/Glc and the isomeric Gal beta 1-4GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-4GlcNAc/Glc.
Four radiolabeled pentasaccharides, GlcNAc beta 1-3(Gal beta 1-4GlcNAc beta 1-6)Gal beta 1-4GlcNAc, Gal beta 1-4GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-4GlcNAc, GlcNAc beta 1-3(Gal beta 1-4GlcNAc beta 1-6)Gal beta 1-4Glc, and Gal beta 1-4GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-4Glc, were prepared in virtually pure form. They were obtained by partial enzymic beta 1,4-galactosylations of the appropriate tetrasaccharide acceptors or by partial enzymic degalactosylations of the appropriate hexasaccharides, followed by paper chromatographic separations. All four pentasaccharides contain two nonidentical distal branches, making them valuable primers for enzymatic in vitro synthesis of larger oligo(N-acetyllactosaminoglycans).
Renkonen
O
O
Institute of Biotechnology, University of Helsinki, Finland.
Leppänen
A
A
Niemelä
R
R
Vilkman
A
A
Helin
J
J
Penttilä
L
L
Maaheimo
H
H
Seppo
A
A
Suopanki
J
J
D000606:Amino Sugars / Q000096:biosynthesis* / Q000737:chemistry; D002240:Carbohydrate Sequence; D005690:Galactose / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry
D000606:Amino Sugars; D009844:Oligosaccharides; D011134:Polysaccharides; C039448:lactosaminoglycan; D005700:Galactosyltransferases; D005690:Galactose