title: The kinetics of the acrosome reaction of human spermatozoa and its correlation with in vitro fertilization.
abstract: OBJECTIVE
  To study the reaction pattern of acrosome reaction in human semen and correlate it to the results of in vitro fertilization (IVF).
  
  
  METHODS
  The percentage of acrosome-reacted spermatozoa of 41 IVF semen samples was determined after 0, 2, 4, and 24 hours of incubation in human tubal fluid medium supplemented with 10% human pool serum.
  
  
  METHODS
  St. Radboud Hospital, Catholic University of Nijmegen, The Netherlands.
  
  
  METHODS
  Forty-one IVF couples.
  
  
  METHODS
  None.
  
  
  METHODS
  Acrosome reaction was determined using fluorescein isothiocyanate conjugated concanavalin A lectin. To avoid false-positive signals from dead spermatozoa, the sperm viability was determined.
  
  
  RESULTS
  Three kinetic patterns of acrosome reaction could be distinguished: (1) normal reacting pattern (percentage of acrosome-reacted spermatozoa less than 10% at 2 hours and greater than 5% at 4 hours; 75% fertilization in IVF); (2) a quickly reacting pattern (percentage of acrosome-reacted spermatozoa greater than 10% at 2 hours; 22% fertilization in IVF); and (3) a nonreacting pattern (percentage of acrosome-reacted spermatozoa less than 5% at all time intervals studied; 15% fertilization in IVF).
  
  
  CONCLUSIONS
  The timing of acrosome reaction and the percentage of acrosome-reacted spermatozoa are very important parameters in IVF.
authors.affiliation: Laboratory of Endocrinology and Reproduction, St. Radbound Hospital, Catholic University Nijmegen, The Netherlands.
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: A M
authors.initials: AM
authors.lastname: Wetzels
authors.forename: H J
authors.initials: HJ
authors.lastname: Goverde
authors.forename: B A
authors.initials: BA
authors.lastname: Bastaans
authors.forename: H J
authors.initials: HJ
authors.lastname: Janssen
authors.forename: R
authors.initials: R
authors.lastname: Rolland
mesh_terms: D000177:Acrosome / Q000502:physiology*; D000704:Analysis of Variance; D004715:Endometriosis / Q000503:physiopathology; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D012661:Semen / Q000502:physiology; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*