Identification and characterization of a specific autoantiphosphatidylinositol immune response during the time course of benzo(a)pyrene-induced malignant tumors in female Sprague-Dawley rats.
High levels of anti-phosphatidylinositol (PtdIns) autoantibodies (autoAb) have been previously described in sera of cancer patients and in plasma of dimethylbenzanthracene-treated female Sprague-Dawley rats. The presence of anti-PtdIns autoAb was tested in a model of highly malignant sarcomas induced by a single dose of benzo(a)pyrene [B(a)P] diluted in sesame oil and injected in female Sprague-Dawley rats. Significantly elevated levels of anti-PtdIns autoAb were found in sera of B(a)P-treated rats 40 days after B(a)P administration, whereas no significant levels of anti-PtdIns autoAb were noted in oil- or benzo(e)pyrene-treated rats. After day 60, autoantibody levels plateaued in B(a)P-treated rats, and highly malignant sarcomas appeared with 100% efficiency around day 100. Anti-PtdIns autoAb avidity and specificity were found to be high.
Faiderbe
S
S
Laboratoire d'Immunologie et Pathologie, Université de Bordeaux II, France.
Chagnaud
J L
JL
Geffard
M
M
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D001564:Benzo(a)pyrene; D001580:Benzopyrenes; D002455:Cell Division / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D010716:Phosphatidylinositols / Q000276:immunology*; D011237:Predictive Value of Tests; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000912:Antibodies, Neoplasm; D001323:Autoantibodies; D001580:Benzopyrenes; D010716:Phosphatidylinositols; D001564:Benzo(a)pyrene; C026487:benzo(e)pyrene