Development of DNA probes for early diagnosis and epidemiological study of cryptococcosis in AIDS patients.
We report the isolation of middle-repetitive DNA sequences from Cryptococcus neoformans that are species and variety specific. These probes were used for assessing strain relatedness among cryptococcal isolates from patients with and without AIDS who were from Zaire and the United States. Five distinct hybridization patterns were observed for the 60 isolates examined, regardless of the restriction enzyme used for digestion. The most common pattern among the isolates from the patients without AIDS was also the most common among the isolates from the patients with AIDS who were from the United States and was the only pattern observed for all isolates tested from patients with AIDS who were from Zaire. On the basis of the high specificity and sensitivity of the signals observed by hybridization, we suggest that these sequences provide a means for both biotyping and early diagnosis of C. neoformans.
Polacheck
I
I
Department of Clinical Microbiology and Infectious Diseases, Hadassah Medical Center, Jerusalem, Israel.
Lebens
G
G
Hicks
J B
JB
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D001483:Base Sequence; D003453:Cryptococcosis / Q000150:complications* / Q000175:diagnosis / Q000453:epidemiology; D003454:Cryptococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D015336:Molecular Probe Techniques / Q000706:statistics & numerical data; D008969:Molecular Sequence Data; D009894:Opportunistic Infections / Q000150:complications* / Q000175:diagnosis / Q000453:epidemiology; D012680:Sensitivity and Specificity; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial