Polyester film strips coated with photographic gelatin containing immobilized glucose oxidase hardened by chromium(III) sulphate.
Glucose oxidase was immobilized into photographic gelatin hardened by chromium(III) sulphate. The enzyme-gelatin mixture was coated on polyester film strips which allowed easy and simple handling during assays. The effect of gelatin and cross-linker concentrations on water content and enzymatic activity was studied. The effect of pH during immobilization and that of incubation temperature on maximum activity were examined. Enzyme leakage tests were carried out during reuse number studies. Consecutive use of strips followed by washing and resting between uses were found to affect the reuse number. A maximum immobilization of 68% was reached under optimal conditions. Mechanical stability and leakage were found to be functions of gelatin and cross-linker concentrations. Photographic gelatin was found to have many capabilities with extraordinary characteristics as a carrier on immobilization.
Elçin
Y M
YM
Chemistry Department, Faculty of Sciences, Ankara University, Turkey.
Akbulut
U
U
D001672:Biocompatible Materials; D002857:Chromium; D017608:Chromium Compounds; D003432:Cross-Linking Reagents; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D005780:Gelatin; D005949:Glucose Oxidase; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D011091:Polyesters; D013431:Sulfates; D013696:Temperature
D001672:Biocompatible Materials; D017608:Chromium Compounds; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D011091:Polyesters; D013431:Sulfates; D002857:Chromium; C040874:chromium sulfate; D005780:Gelatin; D005949:Glucose Oxidase