Human fibroblast cells synthesize and secrete nerve growth factor in culture.
Using our enzyme immunoassay system developed for recombinant hNGF, we examined the synthesis and secretion of human NGF (hNGF) by human fibroblast (WS-1) cells. The amount of the factor secreted by WS-1 cells increased linearly and a significant amount of NGF was detected in the conditioned medium of WS-1 cultures. WS-1 NGF showed properties identical to those of recombinant human NGF in immunoreactivity and molecular weight. An increase in cell density or the withdrawal of serum from the culture medium caused a drastic decrease in the rate of NGF secretion. These results suggest that WS-1 cells are able to synthesize and secrete hNGF in culture and that the synthesis/secretion is regulated in a growth phase-dependent manner.
Murase
K
K
Department of Molecular Biology, Gifu Pharmaceutical University, Japan.
Murakami
Y
Y
Takayanagi
K
K
Furukawa
Y
Y
Hayashi
K
K
D002455:Cell Division; D002460:Cell Line; D002850:Chromatography, Gel; D005347:Fibroblasts; D006801:Humans; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis
D009414:Nerve Growth Factors; D011994:Recombinant Proteins