Immunoglobulin V regions and epitope mapping of a murine monoclonal antibody against p24 core protein of HIV-1.
The nucleotide sequence of a murine monoclonal antibody (CB-mab-p24/13-5) against p24 core protein of the human immunodeficiency virus (HIV-1) was determined for variable regions of the heavy and light chain, respectively. Genetic elements encoding the VDJH- and VJL-regions of the antibody were generated from RNA by the polymerase chain reaction, cloned into the vector pICEM 19R and sequenced. Synthetic peptides, 10 amino acids overlapping served for the localization of the epitope. The residues 152-156 within the p24 sequence contain the epitope.
Küttner
G
G
Institute of Medical Immunology, Humboldt-University, Berlin, Germany.
Giessmann
E
E
Niemann
B
B
Winkler
K
K
Grunow
R
R
Hinkula
J
J
Rosen
J
J
Wahren
B
B
von Baehr
R
R
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D001483:Base Sequence; D016655:HIV Core Protein p24 / Q000235:genetics*; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D016655:HIV Core Protein p24; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region