title: Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis.
abstract: We have previously described a 13- to 15-kDa T-lymphocyte-specific chemotactic protein (endothelial cell-derived lymphocyte chemoattractant activity, ED-LCA) secreted by serotonin-stimulated bovine aortic endothelial cells. In the current study, we have identified a similar serotonin-induced chemotaxin secreted by human aortic endothelial cells (HAEC). Like the bovine ED-LCA, secretion of this human T-cell chemotaxin peaked at 10(-5) M serotonin, was blocked by 5-HT2-receptor antagonists, and was not induced by other vasoactive amines, such as histamine or angiotensin II. In addition, human ED-LCA had no effect on neutrophil or monocyte migration. Using HAEC and human pulmonary arterial endothelial cells (HPAEC) from the same individual, we found that serotonin-stimulated HAEC, but not HPAEC, secreted ED-LCA. Because human vascular endothelium affected by atherosclerosis is morphologically, ultrastructurally, and phenotypically distinct from unaffected areas, we evaluated the secretion of this cytokine from cultured HAEC derived from areas of aorta differentially affected by atherosclerosis. We found that the degree of atherosclerotic involvement of an individual vessel was associated with a decrease in the uptake of serotonin and a reduction in serotonin-induced ED-LCA secretion. In response to serotonin, HAEC derived from atherosclerotic plaques did not secrete ED-LCA, whereas HAEC derived from fatty streaks secreted lesser amounts of ED-LCA than HAEC derived from normal areas. These studies demonstrate that in vivo morphological heterogeneity of HAEC is maintained in vitro and is associated with alterations in function, as measured by cytokine secretion.
authors.affiliation: Pulmonary Center, Boston University School of Medicine, Massachusetts 02118.
authors.forename: H W
authors.initials: HW
authors.lastname: Farber
authors.forename: A S
authors.initials: AS
authors.lastname: Antonov
authors.forename: Y A
authors.initials: YA
authors.lastname: Romanov
authors.forename: V N
authors.initials: VN
authors.lastname: Smirnov
authors.forename: L M
authors.initials: LM
authors.lastname: Scarfo
authors.forename: D J
authors.initials: DJ
authors.lastname: Beer
mesh_terms: D001011:Aorta / Q000378:metabolism* / Q000473:pathology; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000378:metabolism; D002850:Chromatography, Gel; D016207:Cytokines / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D010641:Phenotype; D011651:Pulmonary Artery / Q000378:metabolism / Q000473:pathology; D012701:Serotonin / Q000494:pharmacology
chemical_list: D002630:Chemotactic Factors; D016207:Cytokines; D012701:Serotonin