Enrichment of primary lymphocyte-supporting stromal cells and characterization of associated B lymphocyte progenitors.
Studies of Whitlock/Witte long-term bone marrow cultures have revealed the necessity of two cell types for B lymphopoiesis, a stem cell and the stromal cell. While a number of stromal cell lines exist they have been found to be heterogeneous with respect to cell surface marker expression and growth factor production. Separation and analysis of fresh bone marrow stromal cells is, therefore, necessary to understand the regulation of lymphopoiesis in vivo. Here we report the early stages of such studies. We demonstrate that stromal cells, as assessed by morphology and alkaline phosphatase reactivity after short-term culture, are enriched in cellular aggregates that can be separated from bone marrow suspensions. Stromal cells are present in aggregates at a frequency of one per thousand cells, whereas marrow from which the aggregates have been removed contains only one stromal cell per fifty-thousand cells. These aggregates are able to form Whitlock cultures from greatly reduced numbers of initiating cells, indicating that they contain culturable B lineage precursors as well as stromal cells capable of supporting B lymphopoiesis. The aggregates appear to be naturally formed and provide a means to examine native B cell precursor-stromal cell contacts. We find little evidence for sequestering of late-stage B cell precursors within the aggregates. Terminal deoxynucleotidyl transferase-positive cells, on the other hand, are approximately three times more frequent in bone marrow aggregates, suggesting close contact between very early B cell progenitors and stromal cells within the aggregates. The finding that stromal cells are enriched in cellular aggregates is an important first step in the ultimate isolation of these cells from marrow suspensions, which is vital to understanding stromal cell function in vivo.
Funk
P E
PE
Department of Microbiology and Immunology, Loyola University Medical Center, Maywood, IL 60153.
Witte
P L
PL
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D001854:Bone Marrow Cells; D002449:Cell Aggregation; D005260:Female; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D015851:Interleukin-7 / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D013936:Thymidine / Q000378:metabolism
D015851:Interleukin-7; D013936:Thymidine