title: Monoclonal antibodies to a specific 54-kilodalton antigen of Nocardia spp.
abstract: Two monoclonal antibodies (MAbs) of the immunoglobulin G2A isotype, reacting with a Nocardia-specific 54-kDa antigen, were generated. As determined by Western blot (immunoblot), both MAbs reacted only with the 54-kDa band. As determined by indirect immunofluorescence or enzyme immunoassay with whole microorganisms, the MAbs did not react with Nocardia cells. One of the MAbs showed weak cross-reactivity with mycobacterial antigens, while the other showed no cross-reactivity.
authors.affiliation: Unité de Mycologie, Institut Pasteur, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Boiron
authors.forename: D
authors.initials: D
authors.lastname: Stynen
authors.forename: G
authors.initials: G
authors.lastname: Belkacem
authors.forename: A
authors.initials: A
authors.lastname: Goris
authors.forename: F
authors.initials: F
authors.lastname: Provost
mesh_terms: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000737:chemistry; D006801:Humans; D007159:Immunologic Tests / Q000592:standards / Q000706:statistics & numerical data; D008970:Molecular Weight; D009615:Nocardia / Q000276:immunology*; D009617:Nocardia Infections / Q000175:diagnosis; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial