Induction of myogenic differentiation in human rhabdomyosarcoma cells by ionising radiation, N,N-dimethylformamide and their combination.
Differentiation-inducing ability of gamma-radiation, N,N-dimethylformamide and their combination has been tested on human rhabdomyosarcoma RMZ-RC2 clone cells. Ionising radiation at 2-5 Gy doses induced a more differentiated morphology, with the appearance of an increased proportion of multinuclear myotube-like cells, and a significant increase in myosin-positive and multinuclear cells. Radiation appeared to act by inducing de novo differentiated elements. N,N-dimethylformamide was able to induce an increased myosin expression, but did not affect multinuclear cell proportion. The combined treatment (ionising radiation and N,N-dimethylformamide) resulted in an additive increase in the proportion of myosin-positive cells, approaching 25-35%, but de novo differentiated elements were not increased above the levels obtained with irradiation alone.
Nicoletti
G
G
Institute of Cancerology, University of Bologna, Italy.
De Giovanni
C
C
Landuzzi
L
L
Simone
G
G
Rocchi
P
P
Nanni
P
P
Lollini
P L
PL
D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects; D004126:Dimethylformamide / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D009132:Muscles / Q000166:cytology*; D011650:Pulmonary Alveoli / Q000473:pathology; D012208:Rhabdomyosarcoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
D004126:Dimethylformamide