Optical analysis of synaptic vesicle recycling at the frog neuromuscular junction.
The fluorescent dyes FM1-43 and RH414 label motor nerve terminals in an activity-dependent fashion that involves dye uptake by synaptic vesicles that are recycling. This allows optical monitoring of vesicle recycling in living nerve terminals to determine how recycled vesicles reenter the vesicle pool. The results suggest that recycled vesicles mix with the pool morphologically and functionally. One complete cycle of release of transmitter, recycling of a vesicle, and rerelease of transmitter appears to take about 1 minute.
Betz
W J
WJ
Department of Physiology, University of Colorado School of Medicine, Denver 80262.
Bewick
G S
GS
D000818:Animals; D004558:Electric Stimulation; D005071:Evoked Potentials; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence; D009046:Motor Neurons / Q000502:physiology; D009469:Neuromuscular Junction / Q000502:physiology* / Q000648:ultrastructure; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D011898:Ranidae; D013572:Synaptic Vesicles / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors
C073804:FM1 43; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; C054158:RH 414