Determination of idebenone in rat serum and brain by high-performance liquid chromatography using platinum catalyst reduction and electrochemical detection.
A high-performance liquid chromatographic determination of idebenone, a new cerebral metabolism-improving agent, in rat serum and brain has been developed. After separation of idebenone on a reversed-phase column, idebenone was reduced once in a platinum catalyst reduction column connected on-line, then monitored quantitatively by electrochemical detection. A linear relationship between the peak-height ratio of idebenone to the internal standard and idebenone concentration was observed in the range 0.015-50 ng with a detection limit of 5 pg (signal-to-noise ratio = 5). This method was satisfactorily rapid and sensitive, and was successfully applied to the determination of idebenone in rat serum and brain tissues.
Wakabayashi
H
H
Department of Analytical Chemistry, Niigata College of Pharmacy, Japan.
Nakajima
M
M
Yamato
S
S
Shimada
K
K
D000818:Animals; D016227:Benzoquinones / Q000032:analysis / Q000097:blood*; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D007202:Indicators and Reagents; D008297:Male; D010084:Oxidation-Reduction; D010984:Platinum; D051381:Rats; D011919:Rats, Inbred Strains; D014451:Ubiquinone / Q000031:analogs & derivatives; D014812:Vitamin K / Q000032:analysis
D016227:Benzoquinones; D007202:Indicators and Reagents; D014812:Vitamin K; D014451:Ubiquinone; D010984:Platinum; C036619:idebenone