Negative selection of Tcra-V8+CD8+ T cells by MHC class I molecules.
Tcrb-V-specific positive and negative selection of T cells has been well documented. In contrast, nothing is known about Tcra-V-specific selection. Using Tcra-V8-specific KT50 antibody Tcra-V8-specific selection of T cells has been examined. The CD8+ T cell subpopulation bearing Tcra-V8 are shown to be negatively selected by major histocompatibility complex (MHC) class I H-2Kd and H-2Dd/Ld molecules. Furthermore, percentages of these T cells are also influenced by Tcra-V haplotypes. Involvement of non-H-2 self (super)antigens in this MHC class I restricted negative selection, however, remains to be determined.
Tomonari
K
K
Transplantation Biology Section, MRC Clinical Research Centre, Middlesex, England.
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis*; D005434:Flow Cytometry; D006183:H-2 Antigens / Q000276:immunology*; D006239:Haplotypes; D051379:Mice; D011948:Receptors, Antigen, T-Cell; D013601:T-Lymphocytes / Q000276:immunology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D006183:H-2 Antigens; D011948:Receptors, Antigen, T-Cell