title: Isolation and primary structure of a novel chromogranin A-derived peptide, WE-14, from a human midgut carcinoid tumour.
abstract: The primary structure of a novel human chromogranin A-derived tetradecapeptide, WE-14, possessing N-terminal tryptophanyl (W) and C-terminal glutamyl (E) residues was isolated from a hepatic metastasis of an human ileal carcinoid tumour. Human and bovine WE-14 are structurally identical, while rat, mouse and porcine analogues exhibit 93% homology. WE-14 is flanked by paired basic residues (KR) in all known chromogranin A sequences.
authors.affiliation: Department of Medicine, Queen's University of Belfast, N. Ireland, UK.
authors.forename: W J
authors.initials: WJ
authors.lastname: Curry
authors.forename: C
authors.initials: C
authors.lastname: Shaw
authors.forename: C F
authors.initials: CF
authors.lastname: Johnston
authors.forename: L
authors.initials: L
authors.lastname: Thim
authors.forename: K D
authors.initials: KD
authors.lastname: Buchanan
mesh_terms: D000595:Amino Acid Sequence; D002276:Carcinoid Tumor / Q000378:metabolism* / Q000473:pathology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D053379:Chromogranin A; D002864:Chromogranins / Q000378:metabolism*; D006801:Humans; D007414:Intestinal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics* / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational; D011863:Radioimmunoassay
chemical_list: C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D009363:Neoplasm Proteins; C506155:chromogranin A, mouse; C506153:chromogranin A, rat; C074381:chromogranin A-derived peptide, WE-14