Effects of temperature, potassium concentration, and sugar on human spermatozoa motility: a cell preservation model from reproductive medicine.
Two experiments were performed in vitro with human sperm from a panel of 11 donors. In both experiments, washed sperm from each donor were divided into four equivolume samples, three of which were resuspended in various formulations of Tyrode's solution and the fourth was resuspended in its own seminal plasma. Each of the four samples was then stored at 37 or 3 degrees C for fixed intervals of 0, 2, 6, 12, and 24 h. Motility was assessed at 37 degrees C for all samples at the end of each interval. The results indicate that sperm survival in vitro at 3 degrees C was significantly enhanced by 20 mM K+ in Tyrode's solution relative to Tyrode's with less K+. A metabolizable sugar such as glucose was essential to maintaining sperm viability in K(+)-free media. The addition of raffinose to media containing glucose improved motility of sperm stored at 3 degrees C for 6 h.
Karow
A M
AM
Xytex Corporation, Augusta, Georgia 30904.
Gilbert
W B
WB
Black
J B
JB
D000293:Adolescent; D000328:Adult; D002241:Carbohydrates / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D011188:Potassium / Q000008:administration & dosage; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology; D013696:Temperature
D002241:Carbohydrates; D003470:Culture Media; D011188:Potassium