Measurement of gastrin and transforming growth factor alpha messenger RNA levels in colonic carcinoma cell lines by quantitative polymerase chain reaction.
A synthetic DNA template has been constructed that is suitable for the quantitation of mRNAs encoding gastrin, transforming growth factor alpha (TGF alpha), cholecystokinin, and the 78-kDa gastrin-binding protein. The template was used to measure levels of gastrin and TGF alpha mRNA in 7 colonic and 2 gastric carcinoma cell lines by the polymerase chain reaction. All lines produced detectable gastrin and TGF alpha mRNA with amounts varying between 2.1 and 540 molecules of gastrin mRNA/10(3) cells and 1.1 and 28 molecules of TGF alpha mRNA/10(3) cells. These results are consistent with the hypothesis that both gastrin and TGF alpha act as autocrine growth factors in colon carcinoma cell lines.
Baldwin
G S
GS
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, Victoria, Australia.
Zhang
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QX
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003110:Colonic Neoplasms / Q000737:chemistry*; D006224:Cricetinae; D005755:Gastrins / Q000032:analysis*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000737:chemistry; D012334:RNA, Neoplasm / Q000032:analysis*; D013274:Stomach Neoplasms / Q000737:chemistry; D016211:Transforming Growth Factor alpha / Q000032:analysis*; D014407:Tumor Cells, Cultured
D005755:Gastrins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016211:Transforming Growth Factor alpha