title: Polyamine N-acetyltransferase from Fasciola hepatica.
abstract: A cytosolic polyamine N-acetyltransferase which catalyses polyamine and diamine acetylation has been partially purified from the liver fluke Fasciola hepatica. The enzyme has an apparent Mr of 50,000 and unlike the corresponding mammalian liver counterpart is capable of putrescine acetylation. Among the substrates tested, spermidine had the highest reaction rate but putrescine had a lower Km value. The Km values for spermidine, spermine, norspermidine, putrescine, cadaverine and 1,3-diaminopropane were 20 microM, 1.30 mM, 20 microM, 7 microM, 10 microM and 50 microM, respectively. Acetylated polyamines were also substrates for the trematode acetylase, but histones were inactive. The partially purified enzyme had no deacetylase activity. The Km for acetyl-CoA was 4.4 microM. Coenzyme A was strongly inhibitory with a Ki value of 5.3 microM. Bis(benzyl)polyamine analogue MDL 27695 was a potent competitive inhibitor of the enzyme with a Ki of 22 microM. Inhibition by 1,4-dimethyl-putrescine was non-competitive and had a Ki value of 15 microM. The trematode acetylase is highly dependent on sulfhydryl groups for its activity. As had been reported in nematodes, polyamine acetylation could represent a process by which trematodes convert excess polyamines to forms suitable for transport and excretion. On the other hand, this could be the regulatory step of a functional interconversion pathway in these parasites.
authors.affiliation: Department of Biochemical Parasitology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, F.R.G.
authors.forename: S O
authors.initials: SO
authors.lastname: Aisien
authors.forename: R D
authors.initials: RD
authors.lastname: Walter
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D005210:Fasciola hepatica / Q000201:enzymology*; D007526:Isoelectric Point; D007700:Kinetics; D008970:Molecular Weight
chemical_list: D000123:Acetyltransferases; C030992:polyamine N-acetyltransferase