Evaluation of the 111In-(Sn)-citrate method to label antibodies for radioimmunotargeting studies.
Monoclonal antibody against carcinoembryonic antigen (CEA) was labeled by the 111In-(Sn)-citrate technique and its characteristics were determined from the point of radioimmunotargeting studies. Antibodies of high specific radioactivity could be labeled with a high labeling yield of greater than 90% without any further purification step, and without loss of immunoreactivity. In biodistribution studies using nude mice bearing CEA-producing tumors, an extremely high tumor-to-blood ratio (40 on day 6) was obtained since 111In in the blood cleared very rapidly, but nonspecific 111In localized in the liver and spleen should be seriously considered.
Nakamura
K
K
Department of Radiology, School of Medicine, Keio University, Tokyo, Japan.
Ruiz-Juvera
A
A
Kubo
A
A
Hashimoto
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D002951:Citrates / Q000737:chemistry*; D019343:Citric Acid; D007205:Indium Radioisotopes / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016719:Radioimmunodetection / Q000379:methods*; D014001:Tin / Q000737:chemistry*; D017971:Tin Compounds; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D002951:Citrates; D007205:Indium Radioisotopes; D017971:Tin Compounds; C023599:stannous chloride; D019343:Citric Acid; D014001:Tin