Intracellular free calcium concentrations in single taste receptor cells in the guinea pig.
Single, viable taste receptor cells were isolated from the tongue of the guinea pig by enzymatic digestion and mechanical dissociation. The cells could be classified into flask, spindle and intermediate shapes. The intracellular free calcium ion concentrations [(Ca2+)i] of these cells were determined using the Ca2+ sensitive dye fura-2 and digital imaging microscopy. All types of cells produced an irreversible increase in (Ca2+)i upon addition of Ca2+ ionophore ionomycin (1 microM) and denatonium (10 microM). There was no evidence of any increase in (Ca2+)i in the taste receptor cells in nominally Ca2+ free solution, and when stimulated by denatonium (10 microM). When 3 mM CaCl2 was added, the (Ca2+)i remarkably increased. This would suggest that the (Ca2+)i increase in the presence of denatonium mainly depended on calcium influx from the extracellular space. There was no increase in case of high potassium (50 mM and 150 mM) or saccharose (1 mM and 5 mM) stimulation. The hypothesis that the increase in (Ca2+)i controls biochemical mechanisms related to the bitter taste transduction process is worthy of further study.
Orola
C N
CN
Department of Otolaryngology, Kansai Medical University, Osaka, Japan.
Yamashita
T
T
Harada
N
N
Amano
H
H
Ohtani
M
M
Kumazawa
T
T
D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D003196:Computer Graphics; D003470:Culture Media; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000737:chemistry / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D008959:Models, Neurological; D013650:Taste Buds / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D014059:Tongue / Q000166:cytology / Q000294:innervation*
D003470:Culture Media; D002118:Calcium