title: Purification of restriction endonuclease EcoO128I produced by an enteropathogenic Escherichia coli O128Ly3.
abstract: Restriction endonuclease EcoO128I, an isoschizomer of BstEII, was purified from a rough mutant of Escherichia coli O128Ly3. EcoO128I should be more convenient for recombinant DNA applications than BstEII, because of its improved cleavage activity at 37 degrees C.
authors.affiliation: Division of Microbiology, National Institute of Hygienic Sciences, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Miyahara
authors.forename: K
authors.initials: K
authors.lastname: Mise
authors.forename: F
authors.initials: F
authors.lastname: Kimizuka
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Terawaki
mesh_terms: D001483:Base Sequence; D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D007700:Kinetics; D013379:Substrate Specificity
chemical_list: D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific; C032823:GGTNACC-specific type II deoxyribonucleases