title: Antibodies specific to histone H1 inhibit in vitro transcription in isolated mammalian nuclei.
abstract: The issue of whether histone H1 is present in transcriptionally active chromatin has been approached by studying the effect specific anti-H1 antibodies have on in vitro transcription in isolated nuclei. To that end, the incorporation of radioactive RNA precursors into trichloroacetic acid-precipitable material was compared for control nuclei and nuclei that had been preincubated with specific anti-H1 antibody populations (whole sera, affinity-purified immunoglobulins and monovalent Fab fragments). The anti-H1 antibodies significantly and reproducibly inhibited the transcriptional activity in isolated nuclei. Experiments were also performed to exclude the possibility that the inhibition observed was due to some long-distance effect of the binding of the antibodies to chromatin. The results are interpreted as indicating that active gene chromatin does contain histone H1.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
authors.forename: L N
authors.initials: LN
authors.lastname: Srebreva
authors.forename: J S
authors.initials: JS
authors.lastname: Zlatanova
mesh_terms: D000818:Animals; D000918:Antibody Specificity / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000502:physiology; D003850:Deoxyribonuclease I; D006657:Histones / Q000276:immunology / Q000502:physiology*; D007140:Immunoglobulin Fab Fragments; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D012322:RNA Precursors / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D002843:Chromatin; D006657:Histones; D007140:Immunoglobulin Fab Fragments; D012322:RNA Precursors; D003850:Deoxyribonuclease I