Inhibition of invasion of MCF-7/6 human breast carcinoma cells in vitro by Methanobacterium thermoautotrophicum extract.
Precultured embryonic chick heart fragments were confronted in vitro with various invasive tumor cell lines (MCF-7/6 human breast carcinoma variant, BW-O-LiI mouse T-cell lymphoma cells and MO4 virally transformed fetal mouse carcass cells) and with a non-invasive cell line (MCF-7/AZ human breast carcinoma variant). Confronting cultures were incubated in the presence of various methanogenic cofactors: Methanobacterium thermoautotrophicum extract, coenzyme F420, FO (7,8-didemethyl-8-hydroxy-5-deazariboflavin), F+ (5'-phosphate derivative of FO), or methanopterin. Histological analysis revealed that the Methanobacterium thermoautotrophicum extract inhibits invasion of MCF-7/6 human cells. At the moment it is not known which factor in the extract is responsible for this inhibition.
Raemakers-Franken
P C
PC
Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
Bracke
M E
ME
Van Larebeke
N
N
Vyncke
B
B
Mareel
M M
MM
D001943:Breast Neoplasms / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D002465:Cell Movement; D019605:Euryarchaeota / Q000201:enzymology / Q000502:physiology*; D005260:Female; D006801:Humans; D009361:Neoplasm Invasiveness; D012256:Riboflavin / Q000031:analogs & derivatives / Q000494:pharmacology; D014407:Tumor Cells, Cultured
C015084:coenzyme F420; D012256:Riboflavin