Some properties of a partially purified inhibitor of protein synthesis isolated from bovine cornea.
Bovine cornea extracted with 0.154 M NaCl yielded a protein fraction which (i) inhibited protein synthesis in rabbit reticulocyte lysates, and (ii) reduced the incorporation of formyl-methionine from f[35S]Met-tRNA(f) into polypeptides. The inhibition was reversed by millimolar concentrations of glucose 6-phosphate or cAMP and partially reversed by the addition of initiation factor eIF-2. Thus, the corneal inhibitor may act by directly interfering with the activity of eIF-2.
Wu
J M
JM
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
Herp
A
A
D000818:Animals; D002417:Cattle; D003315:Cornea / Q000502:physiology*; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D007700:Kinetics; D011500:Protein Synthesis Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012358:RNA, Transfer, Met; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism; D014020:Tissue Extracts / Q000494:pharmacology*; D014357:Trypsin / Q000494:pharmacology
D015852:Eukaryotic Initiation Factor-2; D011500:Protein Synthesis Inhibitors; D011506:Proteins; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; D014020:Tissue Extracts; C024988:tRNA, formylmethionine-; D014357:Trypsin