title: Diagnostic use of molecular probes before and after marrow transplantation.
abstract: The application of molecular techniques to disease diagnosis and histocompatibility testing before marrow transplantation and to the evaluation of engraftment and immune reconstitution, and to the identification of infectious and neoplastic complications following transplantation has revolutionized the approach to the care of marrow transplant patients. The techniques of restriction fragment length polymorphism analysis using informative restriction enzymes, the Southern blot technique, and of polymerase chain reaction-amplified DNA and oligonucleotide probe hybridization methods now enable the study of donor and host cells at the nucleotide level.
authors.affiliation: Program in Human Immunogenetics, Fred Hutchinson Cancer Research Center, Seattle, Washington.
authors.forename: E W
authors.initials: EW
authors.lastname: Petersdorf
authors.forename: H J
authors.initials: HJ
authors.lastname: Deeg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D006402:Hematologic Diseases / Q000175:diagnosis / Q000628:therapy; D006410:Hematopoiesis; D006650:Histocompatibility Testing; D006801:Humans; D015335:Molecular Probes; D008969:Molecular Sequence Data
chemical_list: D015335:Molecular Probes