Influence of chicken and human lipoproteins on steroidogenesis in granulosa cells of the domestic fowl (Gallus domesticus).
When freshly dispersed granulosa cells from the largest preovulatory follicle were incubated in the presence of very low density (VLDL), low density (LDL), or high density (HDL) lipoproteins isolated from sera of laying hens, production of both basal and LH-stimulated progesterone was significantly increased in a dose-related manner. VLDL, the principal transporter of cholesterol to the ovum, appeared to be the most efficacious. A highly significant potentiation of the steroidogenic action of 8-bromo-cyclic adenosine monophosphate and forskolin was also observed. Human LDL, and especially HDL, caused significant stimulation of progesterone production by these cells. It is suggested that the release of cholesterol from lipoproteins taken up by granulosa cells raises the precursor pool for steroidogenesis. This mechanism is further enhanced by a cyclic AMP-mediated mechanism.
Hertelendy
F
F
Department of Obstetrics and Gynecology, St. Louis University Medical School, Missouri 63110-0250.
Todd
H
H
Molnár
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M
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002645:Chickens / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism*; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008079:Lipoproteins, VLDL / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000494:pharmacology; D011374:Progesterone / Q000096:biosynthesis*
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011374:Progesterone; D007986:Luteinizing Hormone