Anti-platelet action of isoliquiritigenin, an aldose reductase inhibitor in licorice.
The mechanism was studied by which isoliquiritigenin, a new aldose reductase inhibitor purified from licorice (Glycyrrhizae radix), inhibits platelet aggregation. This new agent significantly inhibited the phosphorylation of 40,000- and 20,000-dalton proteins, and inhibited the formation of 12 (S)-hydroxy-5,8,10-heptadecatrienoic acid, 12-hydroxyeicosatetraenoic acid and thromboxane B2. The inhibitory effect of isoliquiritigenin on platelet aggregation in vitro was comparable to that of aspirin. Our findings may indicate that isoliquiritigenin elicits an anti-platelet action by inhibiting not only cyclooxygenase but also lipoxygenase or peroxidase activity in platelets. Isoliquiritigenin also showed an anti-platelet action in vivo. Isoliquiritigenin appears to be the only aldose reductase inhibitor with a significant anti-platelet action. Since the hyperaggregability of platelets has been implicated in the pathogenesis of diabetic complications, isoliquiritigenin may offer a unique benefit as an aldose reductase inhibitor.
Tawata
M
M
Third Department of Internal Medicine, University of Yamanashi Medical School, Japan.
Aida
K
K
Noguchi
T
T
Ozaki
Y
Y
Kume
S
S
Sasaki
H
H
Chin
M
M
Onaya
T
T
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D047188:Chalcones; D005231:Fatty Acids, Unsaturated / Q000097:blood; D006035:Glycyrrhiza / Q000737:chemistry; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D008970:Molecular Weight; D010766:Phosphorylation / Q000187:drug effects; D010946:Plants, Medicinal; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000097:blood
D001798:Blood Proteins; D047188:Chalcones; D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; D010975:Platelet Aggregation Inhibitors; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid; D013929:Thromboxane B2; D002599:Chalcone; C040920:isoliquiritigenin; D000449:Aldehyde Reductase