title: Basic evaluation of an immunoradiometric competitive inhibition assay for sialosyl-Tn antigen in sera in women. Assay conditions and normal values.
abstract: The assay conditions needed for an immunoradiometric competitive inhibition assay of sera in healthy women were studied using the monoclonal antibody TKH2, which is known to recognize specifically sialosyl-alpha 2,6-GalNAc alpha 1-0-serine/threonine (S-Tn) antigen, a mucinous cancer-related antigen. Stable results were obtained with an incubation time of 1.5 hours at room temperature. The intra-assay and inter-assay coefficients of variation were 3.27% and 3.07%, respectively. The mean (+/- standard deviation [SD]) levels of serum S-Tn in 602 healthy women was 21.2 U/ml (+/- 8.4 U/ml). Values showed a normal logarithmic distribution. Although slightly higher levels were seen in postmenopausal compared with premenopausal women, the differences were not significant. The cutoff value of 41 U/ml was determined from data obtained in 602 healthy women; higher levels were observed in only 2%. Serum S-Tn levels were not strongly influenced by Lewis or ABO (H) blood type, smoking, pregnancy, parturition, or phase of menstrual cycle. The use of the S-Tn antigen as a tumor marker for various gynecologic cancers requires study.
authors.affiliation: Department of Obstetrics and Gynecology, Shimane Medical University, Izumo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ryuko
authors.forename: O
authors.initials: O
authors.lastname: Iwanari
authors.forename: S
authors.initials: S
authors.lastname: Nakayama
authors.forename: M
authors.initials: M
authors.lastname: Kitao
authors.forename: K
authors.initials: K
authors.lastname: Karino
authors.forename: J
authors.initials: J
authors.lastname: Endo
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000097:blood*; D015295:Antigens, Tumor-Associated, Carbohydrate; D001667:Binding, Competitive; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology; D008593:Menopause / Q000097:blood / Q000276:immunology; D008875:Middle Aged; D049590:Postpartum Period / Q000097:blood / Q000276:immunology; D011247:Pregnancy / Q000097:blood / Q000276:immunology; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D012016:Reference Values
chemical_list: D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D007983:Lewis Blood Group Antigens; D011933:Reagent Kits, Diagnostic; C055491:sialosyl-Tn antigen