Natural HIV-1 NEF accelerates virus replication in primary human lymphocytes.
HIV-1 NEF genes were isolated directly from peripheral blood lymphocyte DNA of two HIV-1-infected individuals and cloned into an HXB-2-infectious molecular clone. The effect of NEF on virus production in T-cell lines and primary human lymphocytes was studied. Naturally occurring NEF accelerates virus production in primary human lymphocytes, but not in T-cell lines.
de Ronde
A
A
University of Amsterdam, Academic Medical Centre, Amsterdam.
Klaver
B
B
Keulen
W
W
Smit
L
L
Goudsmit
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004279:DNA, Viral; D015693:Gene Products, nef / Q000737:chemistry / Q000235:genetics; D015754:Genes, nef; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000382:microbiology; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016415:Sequence Alignment; D013601:T-Lymphocytes / Q000382:microbiology; D014779:Virus Replication / Q000235:genetics; D054311:nef Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015693:Gene Products, nef; D054311:nef Gene Products, Human Immunodeficiency Virus