title: Exons encoding the highly conserved part of human glutaminyl-tRNA synthetase.
abstract: Aminoacyl-tRNA synthetases are important components of the genetic apparatus. In spite of common catalytic properties, synthetases with different amino acid specificities are widely diverse in their primary structures, subunit sizes, and subunit composition. However, synthetases with given amino acid specificities are well conserved throughout evolution. We have been studying the human glutaminyl-tRNA synthetase possessing a sequence of about 400 amino acid residues (the core region) that is very similar to sequences in the corresponding enzymes from bacteria and yeast. The conserved sequence appears to be essential for the basic function of the enzyme, the charging of tRNA with glutamine. As a first step to a better understanding of the evolution of this enzyme, we determined the coding region for the conserved part of the human glutaminyl-tRNA synthetase. The coding region is composed of eight exons. It appears that individual exons encode defined secondary structural elements as parts of functionally important domains of the enzyme. Evolution of the gene by assembly of individual exons seems to be a viable hypothesis; alternative pathways are discussed.
authors.affiliation: Fakultät für Biologie, Universität Konstanz, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Kaiser
authors.forename: D
authors.initials: D
authors.lastname: Eberhard
authors.forename: R
authors.initials: R
authors.lastname: Knippers
mesh_terms: D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004247:DNA; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D004247:DNA; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase