title: Ontogeny of gene expression of adenosine A2 receptor in the striatum: early localization in the patch compartment.
abstract: The ontogeny of adenosine A2 receptor mRNA and adenosine A2 binding sites distributions was studied by in situ hybridization histochemistry and receptor autoradiography in pre- and post-natal rat striatum, postnatal dog striatum, and a human fetus striatum and compared to that of dopamine D1 and mu opiate receptors. The early postnatal striatum demonstrated heterogeneous distributions of adenosine A2 receptor mRNA and adenosine A2 binding sites with patches of dense labeling corresponding to dopamine D1 and mu opiate receptors enriched zones. This patchy pattern evolved to the homogeneous distribution observed in the adult. The higher intensity of adenosine A2 receptor mRNA enriched patches correspond at the microscopical level to a higher density of labeled neurons in the patches areas and also to a higher level of expression per labeled patches neuron than in the matrix ones. This demonstrates for the first time that differences in patch/matrix receptor density is at least partly linked to different levels of receptor gene expression.
authors.affiliation: Laboratory of Neuropathology and Neuropeptides Research, Université Libre de Bruxelles, Belgium.
authors.forename: S N
authors.initials: SN
authors.lastname: Schiffmann
authors.forename: J J
authors.initials: JJ
authors.lastname: Vanderhaeghen
mesh_terms: D000241:Adenosine; D000818:Animals; D003342:Corpus Striatum / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D004285:Dogs / Q000254:growth & development / Q000378:metabolism; D005333:Fetus / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D011983:Receptors, Purinergic / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D011983:Receptors, Purinergic; D000241:Adenosine