Comparative studies on internalization of gold labelled mistletoe lectin I (MLI), its subunits, and of an immunotoxin into mouse L 1210V leukemia cells.
Using a preembedding electron microscopic technique, the binding and internalization of gold labelled mistletoe lectin I (MLI.Au), its 2 A subunits (MLI-A.Au) and of the B subunit (MLI-B.Au) in murine L 1210V leukemia cells was analysed. Furthermore, the endocytosis of a gold marked immunotoxin (MoAb-16-MLI-A.Au), consisting of a monoclonal antibody (MoAb-16) reacting with L 1210V cells and the cytotoxic A subunits (MLI-A) was detected. The cells were incubated with MLI.Au, MLI-A.Au, MLI-B.Au, or MoAb-16-MLI-A.Au at 37 degrees C for 1, 3, 5, 10, 20 or 30 min, respectively. Remarkable differences were found in the endocytotic pathway and internalization kinetics. The endocytosis of MLI, its subunits and of the immunotoxin has been compared to that of the other ligands in various systems.
Jonas
L
L
Department of Pathology, University of Rostock, Germany.
Walzel
H
H
Bremer
H
H
Wiedlocha
A
A
D000818:Animals; D004705:Endocytosis; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D007939:Leukemia L1210 / Q000378:metabolism*; D046911:Macromolecular Substances; D051379:Mice; D016253:Microscopy, Immunoelectron; D014784:Mistletoe / Q000737:chemistry; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins / Q000378:metabolism*; D010946:Plants, Medicinal; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000276:immunology / Q000378:metabolism*
D037102:Lectins; D046911:Macromolecular Substances; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum