Endothelin stimulates cardiac alpha- and beta- myosin heavy chain gene expression.
The effect of endothelin-1 on cardiac myosin heavy chain gene expression was examined using an isolated neonatal rat myocardial cell culture system. The effects of endothelin-1 on the expression of alpha- and beta- myosin heavy chain genes in the primary rat myocardial cell culture system were examined by S1 nuclease protection analysis. Endothelin-1 was found to stimulate both alpha- and beta- myosin heavy chain gene expression. The 5' flanking regions of both the alpha- and beta- myosin heavy chain gene promoters ligated to a reporter gene, chloramphenicol acetyltransferase, were used to study the effect of endothelin-1 on transcription. Myocardial cells treated with endothelin-1 increased the transcription rate of alpha- and beta- myosin heavy chain genes in a dose-dependent manner. Thus, the hypertrophic effect of endothelin-1 on cardiac myocytes involves augmentation of alpha- and beta- myosin heavy chain gene expression by increasing gene transcription.
Wang
D L
DL
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.
Chen
J J
JJ
Shin
N L
NL
Kao
Y C
YC
Hsu
K H
KH
Huang
W Y
WY
Liew
C C
CC
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000494:pharmacology*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000378:metabolism; D014158:Transcription, Genetic
D015342:DNA Probes; D016232:Endothelins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D015500:Chloramphenicol O-Acetyltransferase; D009218:Myosins