A murine monoclonal antibody directed against the Gerbich 3 blood group antigen.
A murine monoclonal antibody (NaM19-3C4, IgG1, Kappa) was produced from splenocytes of mice immunized with red blood cells. The antibody agglutinated untreated Ge:2,3,4 and Ge:-2,3,4 erythrocytes in indirect antiglobulin test but failed to agglutinate trypsin-treated cells. Gerbich-negative erythrocyte of the Leach- (Ge:-2,-3,-4) and of the Gerbich- (Ge:-2,-3,4) types were not recognized by the antibody. Immunoblotting experiments showed that the antibody bound to glycophorins C and D from control erythrocytes and to the abnormal glycophorin C identified in the Gerbich-negative cells of the Yussef type (Ge:-2,3,4). No binding to the altered glycophorin C from Ge:-2,-3,4 erythrocytes was observed, indicating that the antibody specifically recognized the Ge:3 epitope localized within residues 40-50 of glycophorin C.
Loirat
M J
MJ
Centre Régional de Transfusion Sanguine, Nantes, France.
Gourbil
A
A
Frioux
Y
Y
Muller
J Y
JY
Blanchard
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity / Q000276:immunology; D001789:Blood Group Antigens / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D007519:Isoantigens / Q000032:analysis*; D051379:Mice
D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D007519:Isoantigens