Sodium butyrate alters the response of a human colon carcinoma cell line to transforming growth factor-beta 1.
The effects of sodium butyrate (NaB) on the response of the RCA human colon carcinoma cell line to transforming growth factor-beta 1 (TGF-beta 1) were examined. NaB induced differentiation, as judged by an increase in cellular alkaline phosphatase, in the RCA cells and this differentiation was accompanied by a decreased growth rate. TGF-beta 1 did not significantly alter the growth or state of differentiation of the RCA cells. The growth rate of cells treated simultaneously with NaB and TGF-beta 1 was similar to that of control untreated cells while the alkaline phosphatase levels remained comparable to cells treated with NaB. Addition of TGF-beta 1 to cells grown in the presence of NaB resulted in a stimulation of growth. Cells pretreated with TGF-beta 1 remained sensitive to the growth inhibitory and differentiation inducing effects of NaB. These results suggest that NaB may alter the expression of proteins responsible for a stimulatory signal response to TGF-beta 1 in RCA cells.
Lewis
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LR
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
Levine
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AE
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D004354:Drug Screening Assays, Antitumor; D004790:Enzyme Induction / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000378:metabolism; D006801:Humans; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D002087:Butyrates; D016212:Transforming Growth Factor beta; D020148:Butyric Acid; D004815:Epidermal Growth Factor; D000469:Alkaline Phosphatase