Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin.
We have sequenced a cDNA of urinary stone protein. cDNA sequences show complete homology between urinary stone protein and human osteopontin (bone sialoprotein) (nucleotides 265-886 and 1183-1424). Osteopontin is a recently discovered bone matrix protein which has been implicated in mediating mineral formation within bone extracellular matrix. This result shows that osteopontin is presumably involved in stone formation as stone matrix.
Kohri
K
K
Department of Urology and Biochemistry, School of Medicine, Kinki University, Osaka, Japan.
Suzuki
Y
Y
Yoshida
K
K
Yamamoto
K
K
Amasaki
N
N
Yamate
T
T
Umekawa
T
T
Iguchi
M
M
Sinohara
H
H
Kurita
T
T
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D007668:Kidney / Q000502:physiology; D008969:Molecular Sequence Data; D053495:Osteopontin; D010750:Phosphoproteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012795:Sialoglycoproteins / Q000235:genetics*
D010750:Phosphoproteins; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin; D004247:DNA