title: Requirement for glucose during in vitro culture of sheep preimplantation embryos.
abstract: Glucose utilization by sheep embryos was examined in 8-cell (N = 36) and blastocyst (N = 36) stages, by measuring conversion of [5-3H]glucose to 3H2O. Fifty percent glucose utilization occurred at 0.79 +/- 0.69 mM for 8-cell embryos and -0.06 +/- 0.15 mM for blastocysts. Development of 1- and 2-cell sheep embryos (N = 264) was examined under different glucose concentrations (0, 1.5, 3, or 6 mM) and in the presence or absence of 0.33 mM pyruvate and 3.3 mM lactate (PL). Overall, the presence of glucose was detrimental (P less than 0.001) to embryonic development. By contrast, the presence of pyruvate and lactate was beneficial (P less than 0.001) to development. An interaction was observed between the concentration of glucose and presence or absence of PL (P less than 0.05). An optimum level of glucose occurs at 0-3 mM in the presence of PL (P less than 0.1). Development to the blastocyst stage was observed in medium when supplemented with amino acids and albumin alone. Thus, glucose metabolism is not critical for embryonic development, but beneficial at low concentrations. High concentrations can inhibit development, possibly by inhibiting the tricarboxylic acid (TCA) cycle. Sheep embryos may also be using amino acids as an energy source for development.
authors.affiliation: MAFTechnology, Ruakura Agricultural Centre, Hamilton, New Zealand.
authors.forename: J G
authors.initials: JG
authors.lastname: Thompson
authors.forename: A C
authors.initials: AC
authors.lastname: Simpson
authors.forename: P A
authors.initials: PA
authors.lastname: Pugh
authors.forename: H R
authors.initials: HR
authors.lastname: Tervit
mesh_terms: D000818:Animals; D046508:Culture Techniques; D047108:Embryonic Development; D005314:Embryonic and Fetal Development; D005260:Female; D005947:Glucose / Q000378:metabolism*; D011247:Pregnancy; D012756:Sheep
chemical_list: D005947:Glucose