Enhancing effect of partial gastrectomy on pancreatic carcinogenesis.
The controversial issue of enhanced pancreatic carcinogenesis following partial gastrectomy has been explored in male Wistar rats (n = 40) weighing 250-300 g. Animals were randomised to receive either 60% distal gastrectomy with Roux-en-Y reconstruction or gastrotomy and resuture (control). Immediately after operation each group was further divided into two subgroups, receiving i.p. injections of either saline or azaserine (30 mg kg-1 wk-1 for 3 weeks). At 15 months blood was obtained at 0, 5, 15 and 30 min after a fatty meal for cholecystokinin (CCK) assay; rats were then killed. Pancreatic wet weight was measured, and histological sections were examined for atypical acinar cell foci (AACF), the putative precursor lesion of carcinoma. There were no significant differences in body weight or pancreatic weight between controls and rats with gastrectomy. Only azaserine-treated rats had acidophilic AACF. Partial gastrectomy substantially increased the number of acidophilic AACF per pancreas (median 26.05 vs 2.09; P less than 0.005), with a 9-fold increase in their volume (P less than 0.005). Basal and postprandial plasma CCK concentrations were higher after gastrectomy than in controls (P less than 0.05). Partial gastrectomy has an enhancing effect on azaserine-induced pancreatic carcinogenesis, probably by means of increased CCK release.
Watanapa
P
P
Department of Surgery, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Flaks
B
B
Oztas
H
H
Deprez
P H
PH
Calam
J
J
Williamson
R C
RC
D000713:Anastomosis, Roux-en-Y; D000818:Animals; D001377:Azaserine; D002766:Cholecystokinin / Q000097:blood; D004041:Dietary Fats / Q000378:metabolism; D005500:Follow-Up Studies; D005743:Gastrectomy / Q000009:adverse effects*; D008297:Male; D009374:Neoplasms, Experimental; D009929:Organ Size; D010179:Pancreas / Q000033:anatomy & histology; D010190:Pancreatic Neoplasms / Q000209:etiology*; D011183:Postoperative Complications; D011230:Precancerous Conditions / Q000209:etiology; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000601:surgery
D004041:Dietary Fats; D001377:Azaserine; D002766:Cholecystokinin