Characterization and detoxification of an easily prepared acellular pertussis vaccine. Antigenic role of the A protomer of pertussis toxin.
An acellular pertussis vaccine was prepared from pertussis vaccine concentrates by eliminating the cells by centrifugation and subsequent acid precipitation. SDS-PAGE analysis indicated the presence of 17 clearly defined bands including the subunits of pertussis toxin. The acellular preparation was detoxified with glutaraldehyde and lost its undesirable effects (leukocytosis-promoting activity, histamine-sensitizing activity and decrease in weight gain) but retained a very good protective activity (PD50 approximately 2 micrograms protein/mouse). Sera from mice immunized with this preparation indicated an antibody response against the A protomer of pertussis toxin.
García-Sáinz
J A
JA
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, DF.
Romero-Avila
M T
MT
Ruíz-Arriaga
A
A
Ruíz-Puente
J
J
Agundis
C
C
Ortiz
V
V
Isibasi
A
A
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D005976:Glutaral / Q000494:pharmacology; D051379:Mice; D010567:Pertussis Vaccine / Q000276:immunology*
D000907:Antibodies, Bacterial; D010567:Pertussis Vaccine; D005976:Glutaral