Isolation of two distinct type I angiotensin II receptor genes.
A rat genomic Southern blot, probed with a type I angiotensin II receptor probe, demonstrated that two highly homologous type I angiotensin II receptors were present. A rat genomic library was subsequently screened and four clones were isolated. From restriction mapping, differential hybridization, polymerase chain reaction amplification and sequence analyses we have determined that there are two unique type I angiotensin II receptor genes. The first of these genes corresponds to the published rat vascular complementary DNA sequence; the second, corresponds to a novel receptor not previously described.
Elton
T S
TS
Department of Medicine, University of Alabama, Birmingham 35294.
Stephan
C C
CC
Taylor
G R
GR
Kimball
M G
MG
Martin
M M
MM
Durand
J N
JN
Oparil
S
S
D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D016366:Open Reading Frames; D051381:Rats; D011945:Receptors, Angiotensin / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D004247:DNA