Biological activity in the repopulating rat spermatocyte after the withdrawal of gossypol treatment: III. The activity for the transcription of messenger RNA.
Mature male rats were treated with gossypol for 8 weeks after which the treatment was stopped to allow the arrested spermatogonia to revive. The arrested spermatogonia in the testes were allowed to develop for 15, 30, and 45 days. The amount of histone H2b, H4 and actin messenger RNA (mRNA) in the repopulated pachytene spermatocyte (RPS) and the control pachytene spermatocyte (CPS) was detected by the dot-blot hybridization technique. Histone H2b mRNA in total testicular RNA is higher than that detected in CPS and RPS. The amount of histone H2b mRNA in 5 micrograms to 10 micrograms of poly (A)+ RNA isolated from CPS is 235% higher than that detected in the RPS. The H2b mRNA content in the resting spermatocytes (15, 30, and 45 days after the withdrawal of gossypol treatment) was inhibited by 68, 72, and 45%, respectively. Histone H2b mRNA transcription in the resting spermatocyte of these 3 time periods was inhibited by 26, 40, and 0%, respectively. Fifteen days after the withdrawal of gossypol treatment, the amount of actin mRNA in RPS was inhibited by 47%. The inhibitory effect of gossypol was rapidly recovered within 30 days after the withdrawal of the drug treatment. It was concluded that after the withdrawal of gossypol treatment, the production of H2b, H4, and actin mRNA in RPS was inhibited. However, the inhibitory effect on the mRNAs' production is reversible. The recovery rate for the mRNAs follows the following order: Actin mRNA greater than H4 mRNA greater than H2b mRNA.
Teng
C S
CS
Department of Anatomy, Physiological Science and Radiology North Carolina State University, Raleigh.
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D006072:Gossypol / Q000494:pharmacology*; D006657:Histones / Q000235:genetics / Q000378:metabolism; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013090:Spermatocytes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D013091:Spermatogenesis / Q000187:drug effects; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
D000199:Actins; D006657:Histones; D012333:RNA, Messenger; D006072:Gossypol