Ultrastructural immunocytochemical localization of chondroitin sulfate proteoglycan in Bruch's membrane of the rat.
Two monoclonal antibodies (Mab 4D5 and 2D6) raised against the core protein of a basement membrane chondroitin sulfate proteoglycan from Reichert's membrane of the rat, were used for ultrastructural immunoperoxidase localization of this protein in Bruch's membrane of the rat. Immunoreactivity for both antibodies was found in the basal lamina (basement membrane) of the choriocapillary endothelium and retinal pigment epithelium, in collagen fibers in the collagenous zones, and surrounding the elastic layer.
Lin
W L
WL
Kresge Eye Institute, Wayne State University School of Medicine, Detroit, Michigan.
Essner
E
E
McCarthy
K J
KJ
Couchman
J R
JR
D000818:Animals; D000911:Antibodies, Monoclonal; D001485:Basement Membrane / Q000737:chemistry / Q000648:ultrastructure; D016570:Bruch Membrane / Q000737:chemistry* / Q000648:ultrastructure; D002196:Capillaries / Q000648:ultrastructure; D002809:Chondroitin Sulfates / Q000032:analysis*; D002829:Choroid / Q000098:blood supply; D005260:Female; D007124:Immunoenzyme Techniques; D016253:Microscopy, Immunoelectron; D010857:Pigment Epithelium of Eye / Q000737:chemistry / Q000648:ultrastructure; D011509:Proteoglycans / Q000032:analysis* / Q000648:ultrastructure; D051381:Rats; D011917:Rats, Inbred Lew
D000911:Antibodies, Monoclonal; D011509:Proteoglycans; D002809:Chondroitin Sulfates