title: A heterophilic adhesion mechanism for platelet/endothelial cell adhesion molecule 1 (CD31).
abstract: The molecular nature of cell adhesion mediated by platelet/endothelial cell adhesion molecule 1 (PECAM-1; CD31) was examined using stably transfected L cells in a PECAM-dependent aggregation assay. This adhesion was temperature sensitive and divalent cation dependent, with Mg2+ supporting aggregation to a greater degree than Ca2+. PECAM-dependent aggregation was heterophilic: PECAM-1 transfectants bound as readily to control-transfected L cells as to other PECAM-1 transfectants, demonstrating that a molecule endogenously expressed on the L cells serves as the ligand for PECAM in this system and presumably substitutes for the natural human ligand.
authors.affiliation: Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York 10021.
authors.forename: W A
authors.initials: WA
authors.lastname: Muller
authors.forename: M E
authors.initials: ME
authors.lastname: Berman
authors.forename: P J
authors.initials: PJ
authors.lastname: Newman
authors.forename: H M
authors.initials: HM
authors.lastname: DeLisser
authors.forename: S M
authors.initials: SM
authors.lastname: Albelda
mesh_terms: D000818:Animals; D015214:Antigens, Differentiation, Myelomonocytic / Q000502:physiology*; D002412:Cations; D002448:Cell Adhesion / Q000502:physiology*; D007700:Kinetics; D007739:L Cells; D051379:Mice; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D013696:Temperature; D014162:Transfection
chemical_list: D015214:Antigens, Differentiation, Myelomonocytic; D002412:Cations; D019408:Platelet Endothelial Cell Adhesion Molecule-1