title: Transforming growth factors type-beta and dexamethasone attenuate group II phospholipase A2 gene expression by interleukin-1 and forskolin in rat mesangial cells.
abstract: Treatment of rat mesangial cells with interleukin-1 beta (IL-1 beta) and forskolin induced, in a synergistic fashion, the expression of group II phospholipase A2 (PLA2) mRNA, with subsequent increased synthesis and secretion of PLA2. In contrast, interleukin-6 did not increase PLA2 mRNA levels of PLA2 activity. Transforming growth factor (TGF) beta 1, TGF beta 2 and TGF beta 3 equipotently attenuated the IL-1 beta- and forskolin-induced elevation of PLA2 mRNA, as well as PLA2 synthesis and secretion. The glucocorticoid dexamethasone only partially suppressed the IL-1 beta- and forskolin-induced elevation of PLA2 mRNA, but totally inhibited PLA2 synthesis and secretion.
authors.affiliation: Department of Pharmacology, Biocenter, University of Basel, Switzerland.
authors.forename: H
authors.initials: H
authors.lastname: Mühl
authors.forename: T
authors.initials: T
authors.lastname: Geiger
authors.forename: W
authors.initials: W
authors.lastname: Pignat
authors.forename: F
authors.initials: F
authors.lastname: Märki
authors.forename: H
authors.initials: H
authors.lastname: van den Bosch
authors.forename: N
authors.initials: N
authors.lastname: Cerletti
authors.forename: D
authors.initials: D
authors.lastname: Cox
authors.forename: G
authors.initials: G
authors.lastname: McMaster
authors.forename: K
authors.initials: K
authors.lastname: Vosbeck
authors.forename: J
authors.initials: J
authors.lastname: Pfeilschifter
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D004357:Drug Synergism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005920:Glomerular Mesangium / Q000166:cytology / Q000201:enzymology*; D007375:Interleukin-1 / Q000494:pharmacology*; D010741:Phospholipases A / Q000235:genetics* / Q000378:metabolism; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D005576:Colforsin; D003907:Dexamethasone; D010741:Phospholipases A; D054467:Phospholipases A2