Cloning of a Clostridium botulinum type B toxin gene fragment encoding the N-terminus of the heavy chain.
Two lambda gt11 clones of the toxin gene of Clostridium botulinum type B were identified by the monoclonal antibody specific to the heavy chain of type B toxin. Neither of the expressed fusion proteins from the lysates of lysogenic E. coli Y1089 showed any botulinal toxic activity. One of the clones hybridized to the oligonucleotide probe which was synthesized according to the amino acid sequence of N-terminus of heavy chain. The sequence analysis revealed that highly homologous regions in N-terminus of heavy chain exist among botulinum neurotoxins (type A, B) and tetanus toxin on the amino acid sequence level.
Jung
H H
HH
Department of Life Science, Korea Advanced Institute of Science and Technology, Taejon, Korea.
Rhee
S D
SD
Yang
K H
KH
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D001905:Botulinum Toxins / Q000096:biosynthesis / Q000235:genetics*; D003014:Clostridium botulinum / Q000235:genetics* / Q000472:pathogenicity; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D001905:Botulinum Toxins