Molecular discrimination of Campylobacter coli serogroup 20 biotype I (Lior) strains.
Plasmid, protein and restriction endonuclease analysis (REA) profiles and multilocus enzyme electrophoresis were used to effect a molecular discrimination of twenty-seven Campylobacter coli serogroup 20, biotype 1 (Lior) strains. These strains were not outbreak-associated but were isolated from a number of different countries and different animal and environmental sources. Each of the techniques was able to discriminate, to various degrees, between the serogroup 20, biotype 1 strains. The choice of a particular technique depends to a large extent on the level of discrimination desired, the previous experiences of the investigator and on the laboratory facilities at hand. REA profiles demonstrated the greatest degree of discrimination between these strains. Plasmid and protein profiles could discriminate reasonably well. Multilocus enzyme electrophoresis (allozyme typing) and protein profiles may prove effective in subgrouping serogroup 20, biotype 1 strains.
Fraser
A D
AD
Agriculture Canada, Animal Diseases Research Institute, NEPEAN, Ontario.
Brooks
B W
BW
Garcia
M M
MM
Lior
H
H
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry; D015373:Bacterial Typing Techniques; D017000:Campylobacter coli / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008970:Molecular Weight; D010957:Plasmids; D000091402:Prohibitins; D015183:Restriction Mapping
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C000717331:PHB2 protein, human; D000091402:Prohibitins