Determination of mephenytoin stereoselective oxidative metabolism in urine by chiral liquid chromatography employing beta-cyclodextrin as a mobile phase additive.
A direct high-performance liquid chromatographic assay was developed for the separation and determination of S- and R-mephenytion enantiomers in human urine. Separation was achieved on a Supelcosil LC-8 column with methanol-0.1 M acetate buffer as the mobile phase and beta-cyclodextrin as a mobile phase additive. The urinary S/R enantiomeric ratio of mephenytoin was measured in a 32-h urine sample (as phenotypic traits) for determination of the ability of normal subjects to hydroxylate racemice mephenytoin after oral administration of the drug.
Róna
K
K
Postgraduate Medical School I, Department of Medicine, Budapest, Hungary.
Szabó
I
I
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins; D006801:Humans; D008617:Mephenytoin / Q000378:metabolism* / Q000652:urine; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C031215:betadex; D008617:Mephenytoin