A rapid method for purification of herpesvirus DNA.
A rapid and reliable method for purification of herpesvirus DNA from cell cultures is described. The method is based on the isolation of virus particles and/or nucleocapsids by differential centrifugation and exploits the solubilizing and denaturing capabilities of cesium trifluoroacetate during isopycnic centrifugation, so that phenol/chloroform extractions can be omitted. The method was used for the purification of DNA from several members of the Alfaherpesvirinae subfamily.
Christensen
L S
LS
State Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark.
Normann
P
P
D002478:Cells, Cultured; D004279:DNA, Viral / Q000302:isolation & purification*; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral