Bioassay of organophosphate nerve agents in soil using neuronal tissue cultures.
A soil sample originating from an area of suspected chemical warfare activity was subjected to chemical analysis and bioassay. Sarin and several related compounds were confirmed in the soil by capillary column gas chromatography-mass spectrometry (GC-MS); however, the binding of these compounds to the soil hindered quantitation. The chemical results were then compared to those obtained by bioassay in primary cultures of chick embryo forebrain neurons. By comparing the sample's anticholinesterase activity against those of purified standards in chick embryo neuron cultures, a reasonable agreement was found between the chemical and bioassay semi-quantitative estimates of sarin content in the soil extract. Furthermore, the in vitro system appears to offer a sensitive technique for the estimation of sarin remaining bound to the soil following solvent extraction as well as for an assessment of the potential toxicity of the contaminated soil in vivo.
Sawyer
T W
TW
Defence Research Establishment Suffield, Alberta, Canada.
Weiss
M T
MT
D'Agostino
P A
PA
Provost
L R
LR
Hancock
J R
JR
D000818:Animals; D002619:Chemical Warfare Agents / Q000032:analysis*; D002642:Chick Embryo; D002800:Cholinesterase Inhibitors / Q000032:analysis; D046508:Culture Techniques; D008401:Gas Chromatography-Mass Spectrometry; D008752:Methylene Chloride; D009466:Neuromuscular Blocking Agents / Q000032:analysis*; D009474:Neurons / Q000187:drug effects*; D009943:Organophosphorus Compounds / Q000032:analysis*; D012524:Sarin / Q000032:analysis; D016037:Single-Blind Method; D012987:Soil / Q000032:analysis*
D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D009466:Neuromuscular Blocking Agents; D009943:Organophosphorus Compounds; D012987:Soil; D008752:Methylene Chloride; D012524:Sarin