title: The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes.
abstract: The gene encoding Escherichia coli acyl carrier protein (ACP) has been isolated and sequenced. The ACP gene (called acpP) was located on the genetic map between fabF and fabD which encode two fatty acid biosynthetic enzymes, 3-ketoacyl-ACP synthase II and malonyl CoA-ACP transacylase, respectively. An open reading frame between acpP and fabD encodes a 26.5-kDa protein that has significant sequence identity (greater than 40%) with two acetoacetyl-CoA reductases and thus is believed to encode a 3-ketoacyl-ACP reductase. This gene (called fabG) is cotranscribed with acpP. Thus, the gene encoding ACP, the key carrier protein of fatty acid synthesis, is located within a cluster of fatty acid biosynthetic genes.
authors.affiliation: Department of Microbiology, University of Illinois, Urbana-Champaign, Illinois 61801.
authors.forename: M
authors.initials: M
authors.lastname: Rawlings
authors.forename: J E
authors.initials: JE
authors.lastname: Cronan
mesh_terms: D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase; D000213:Acyl Carrier Protein / Q000235:genetics*; D000429:Alcohol Oxidoreductases / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D015723:Gene Library; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D000213:Acyl Carrier Protein; D005227:Fatty Acids; D000429:Alcohol Oxidoreductases; D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase; C023744:acetoacetyl-CoA reductase