Structural analysis on the single-stranded genomic DNAs of the virus newly isolated from silkworm: the DNA molecules share a common terminal sequence.
Recently, a parvo-like virus was newly isolated from silkworm larvae and the two viral DNAs (VD1 and VD2) with different electro-mobilities were identified. We cloned the viral DNAs in a plasmid pUC119 and demonstrated that these two DNAs were not a bimorphic molecules though they shared a common terminal sequence of 53 nucleotides. In addition, the sequence at the 5' terminus of each strand of the viral DNA was located in inverted form at its 3' terminus. On the other hand, the nucleotide sequences of VD1 and VD2 were different from that of the Bombyx densovirus (Ina isolate) DNA.
Bando
H
H
Laboratory of Sericology, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Choi
H
H
Ito
Y
Y
Nakagaki
M
M
Kawase
S
S
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000382:microbiology*; D003001:Cloning, Molecular; D004267:DNA Viruses / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D007304:Insect Viruses / Q000235:genetics*; D008969:Molecular Sequence Data
D004277:DNA, Single-Stranded; D004279:DNA, Viral