title: Determination of cefixime and its metabolites by high-performance capillary electrophoresis.
abstract: Cefixime (CX), an oral cephalosporin antibiotic, and its metabolites in human digestive organs were separated by various modes of high-performance capillary electrophoresis. The zone electrophoresis mode in phosphate buffer (pH 6.8) containing 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulphonate gave the best separation, permitting the complete resolution of CX and all of five metabolites. On the other hand, the plain zone electrophoresis mode in phosphate buffer (pH 6.8) offered a simple procedure for the direct determination of urinary CX concentration using intact urine samples.
authors.affiliation: Faculty of Pharmaceutical Sciences and Pharmaceutical Research, Kinki University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Honda
authors.forename: A
authors.initials: A
authors.lastname: Taga
authors.forename: K
authors.initials: K
authors.lastname: Kakehi
authors.forename: S
authors.initials: S
authors.lastname: Koda
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
mesh_terms: D000892:Anti-Infective Agents, Urinary / Q000378:metabolism* / Q000652:urine; D020682:Cefixime; D002439:Cefotaxime / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D004586:Electrophoresis / Q000379:methods; D006801:Humans
chemical_list: D000892:Anti-Infective Agents, Urinary; D020682:Cefixime; D002439:Cefotaxime