Sequence and characterization of pvuIIR, the PvuII endonuclease gene, and of pvuIIC, its regulatory gene.
An open reading frame partially overlaps pvuIIR, and genetic evidence implies that this open reading frame, named pvuIIC, specifies a positive regulator of pvuIIR (T. Tao, J. C. Bourne, and R. M. Blumenthal, J. Bacteriol. 173:1367-1375, 1991). Inducible constructs of pvuIIC produced a protein of the expected size. The site of C.PvuII action appears to lie within pvuIIC itself; thus, pvuIIC may be a self-contained regulatory cassette.
Tao
T
T
Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.
Blumenthal
R M
RM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005809:Genes, Regulator / Q000235:genetics*; D008969:Molecular Sequence Data; D011514:Proteus vulgaris / Q000235:genetics*; D011994:Recombinant Proteins
D011994:Recombinant Proteins; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific