Evidence for zinc binding by two structural proteins of Plodia interpunctella granulosis virus.
Workers in our laboratory previously reported the possibility of cation involvement in the in vitro dissociation of the Plodia interpunctella granulosis virus nucleocapsids (K. A. Tweeten, L. A. Bulla, Jr., and R. A. Consigli, J. Virol. 33:866-876, 1980; M. E. Wilson and R. A. Consigli, Virology 143:516-525, 1985). The current study found zinc associated with both granulosis virus nucleocapsids and granulin by atomic absorption analysis. A blotting assay with 65Zn2+ specifically identified the radioactive cation as binding to two viral structural proteins, granulin and VP12. These findings indicate that zinc may have a critical role in maintaining virus stability.
Funk
C J
CJ
Division of Biology, Kansas State University, Manhattan 66506.
Consigli
R A
RA
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D016367:Baculoviridae / Q000737:chemistry* / Q000378:metabolism; D009036:Moths / Q000382:microbiology; D013054:Spectrophotometry, Atomic; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000378:metabolism; D014771:Virion / Q000737:chemistry; D015032:Zinc / Q000032:analysis* / Q000378:metabolism
D000596:Amino Acids; D015678:Viral Structural Proteins; D015032:Zinc