title: [Image cytometric DNA analysis in the evaluation of the risk of recurrence after resection of rectal carcinoma].
abstract: Prognostically relevant factors derived from re-operative endoluminal US examination or the histopathological work-up of the surgical specimen in carcinoma of the rectum are well known. We investigated the question as to whether the DNA content of tumor cells is an independent influencing factor of prognosis after resection of rectal carcinoma. In 68 patients who underwent resection for rectal carcinoma, in addition to the TNM classification and the usual morphological evaluation, the paraffin-embedded tumor material was de-paraffinized, rehydrated and treated mechanically and enzymatically prior to application to microscope slides. These smears were analysed with the aid of automatic single cell cytometry, and DNA measurements were performed. In contrast to flow cytometry, this technique permits, on the basis of electronic selection criteria, selective analysis of the tumor cells, while artefacts, stroma and inflammatory cells can be excluded. Using DNA cytometry, we were able to differentiate between diploid, polyploid and aneuploid tumors. The best prognosis was associated with a diploid tumor, the poorest with aneuploid tumor. Regression analysis, however, showed that the DNA content of tumor cells is of only secondary significance.
authors.affiliation: Klinik und Poliklinik für Allgemein- und Abdominalchirurgie, Johannes-Gutenberg-Universität, Mainz.
authors.forename: T
authors.initials: T
authors.lastname: Böttger
authors.forename: H
authors.initials: H
authors.lastname: Gabbert
authors.forename: M
authors.initials: M
authors.lastname: Stöckle
authors.forename: S
authors.initials: S
authors.lastname: Wellek
authors.forename: R
authors.initials: R
authors.lastname: Hills
authors.forename: A
authors.initials: A
authors.lastname: Heintz
authors.forename: T
authors.initials: T
authors.lastname: Junginger
mesh_terms: D003584:Cytological Techniques; D004273:DNA, Neoplasm / Q000032:analysis*; D004171:Diploidy; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D015999:Multivariate Analysis; D009364:Neoplasm Recurrence, Local; D011123:Polyploidy; D011379:Prognosis; D012004:Rectal Neoplasms / Q000175:diagnosis* / Q000601:surgery; D012044:Regression Analysis; D012307:Risk Factors
chemical_list: D004273:DNA, Neoplasm
vernacular_title: Bildzytometrische DNS-Analyse zur Beurteilung des Rezidivrisikos nach Resektion von Rektumkarzinomen.