title: A new highly sensitive immunoassay for cytokines by dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA).
abstract: Non-isotopic immunoassays for human tumor necrosis factor alpha (TNF alpha) and human interleukin-6 (IL-6) were established by employing the dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA) system based on the time-resolved fluoroimmunoassay technique with europium-labeled antibody. Compared to enzyme-linked immunosorbent assays and bioassays, the sensitivity and range of measurement were significantly increased by applying the DELFIA systems to TNF alpha and IL-6. TNF alpha was measurable from 100 fg/ml to 10 ng/ml with the TNF alpha-DELFIA and IL-6 was measurable from 100 fg/ml to 1 ng/ml with the IL-6-DELFIA.
authors.affiliation: Department of Medicine III, Osaka University Medical School, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ogata
authors.forename: H
authors.initials: H
authors.lastname: Tagoh
authors.forename: T
authors.initials: T
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Kuritani
authors.forename: Y
authors.initials: Y
authors.lastname: Takahara
authors.forename: T
authors.initials: T
authors.lastname: Shimamura
authors.forename: H
authors.initials: H
authors.lastname: Ikegami
authors.forename: M
authors.initials: M
authors.lastname: Kurimoto
authors.forename: K
authors.initials: K
authors.lastname: Yoshizaki
authors.forename: T
authors.initials: T
authors.lastname: Kishimoto
mesh_terms: D000911:Antibodies, Monoclonal; D001681:Biological Assay; D016207:Cytokines / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005063:Europium; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D005063:Europium