A new highly sensitive immunoassay for cytokines by dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA).
Non-isotopic immunoassays for human tumor necrosis factor alpha (TNF alpha) and human interleukin-6 (IL-6) were established by employing the dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA) system based on the time-resolved fluoroimmunoassay technique with europium-labeled antibody. Compared to enzyme-linked immunosorbent assays and bioassays, the sensitivity and range of measurement were significantly increased by applying the DELFIA systems to TNF alpha and IL-6. TNF alpha was measurable from 100 fg/ml to 10 ng/ml with the TNF alpha-DELFIA and IL-6 was measurable from 100 fg/ml to 1 ng/ml with the IL-6-DELFIA.
Ogata
A
A
Department of Medicine III, Osaka University Medical School, Japan.
Tagoh
H
H
Lee
T
T
Kuritani
T
T
Takahara
Y
Y
Shimamura
T
T
Ikegami
H
H
Kurimoto
M
M
Yoshizaki
K
K
Kishimoto
T
T
D000911:Antibodies, Monoclonal; D001681:Biological Assay; D016207:Cytokines / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005063:Europium; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D000911:Antibodies, Monoclonal; D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D005063:Europium