title: Guanyl nucleotides modulate binding to steroid receptors in neuronal membranes.
abstract: The recently characterized corticosteroid receptor on amphibian neuronal membranes appears to mediate rapid, stress-induced changes in male reproductive behaviors. Because the transduction mechanisms associated with this receptor are unknown, we performed radioligand binding studies to determine whether this steroid receptor is negatively modulated by guanyl nucleotides. The binding of [3H]corticosterone to neuronal membranes was inhibited by nonhydrolyzable guanyl nucleotides in both equilibrium saturation binding and titration studies. The addition of guanyl nucleotide plus unlabeled corticosterone induced a rapid phase of [3H]corticosterone dissociation from membranes that was not induced by addition of unlabeled ligand alone. Furthermore, the equilibrium binding of [3H]corticosterone and the sensitivity of the receptor to modulation by guanyl nucleotides were both enhanced by Mg2+. These results are consistent with the formation of a ternary complex of steroid, receptor, and guanine nucleotide-binding protein that is subject to regulation by guanyl nucleotides. Therefore, rapid signal transduction through corticosteroid receptors on neuronal membranes appears to be mediated by guanine nucleotide-binding proteins.
authors.affiliation: Department of Zoology, Oregon State University, Corvallis 97331-2914.
authors.forename: M
authors.initials: M
authors.lastname: Orchinik
authors.forename: T F
authors.initials: TF
authors.lastname: Murray
authors.forename: P H
authors.initials: PH
authors.lastname: Franklin
authors.forename: F L
authors.initials: FL
authors.lastname: Moore
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003345:Corticosterone / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000378:metabolism; D015636:Magnesium Chloride / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D012455:Salamandridae; D015398:Signal Transduction
chemical_list: D006150:Guanine Nucleotides; D011965:Receptors, Glucocorticoid; D015636:Magnesium Chloride; D019204:GTP-Binding Proteins; D004229:Dithiothreitol; D003345:Corticosterone