title: Glyoxylic acid in the study of autonomic innervation in the gerbil cochlea.
abstract: The autonomic innervation of the inner ear has been investigated earlier, yet questions concerning the origin, function, extent, and distribution of sympathetic nerves in the cochlea still remain unanswered. This study investigates the extent and distribution of adrenergic nerves in the cochlea. Our procedure combines the glyoxylic acid method of catecholamine fluorophore identification with rapid dissection, decalcification, and whole-mount slide preparation techniques to topographically trace the adrenergic innervation of the cochlear infrastructure. We have demonstrated that perivascular adrenergic innervation extends beyond the immediate branches of the modiolar artery and reaches into radiating arterioles. These findings also suggest the possibility of segmental regulation of cochlear blood flow.
authors.affiliation: Division of Otolaryngology/Head and Neck Surgery, University of North Carolina, Chapel Hill 27599-7070.
authors.forename: H C
authors.initials: HC
authors.lastname: Pillsbury
authors.forename: S
authors.initials: S
authors.lastname: Pulver
authors.forename: V N
authors.initials: VN
authors.lastname: Carrasco
authors.forename: K
authors.initials: K
authors.lastname: Scruggs
authors.forename: D
authors.initials: D
authors.lastname: Carver
authors.forename: L
authors.initials: L
authors.lastname: de Serres
authors.forename: M
authors.initials: M
authors.lastname: Bleynat
authors.forename: J
authors.initials: J
authors.lastname: Prazma
mesh_terms: D000320:Adrenergic Fibers; D000818:Animals; D001341:Autonomic Nervous System / Q000033:anatomy & histology*; D003051:Cochlea / Q000098:blood supply / Q000294:innervation*; D004210:Dissection; D005453:Fluorescence; D005849:Gerbillinae; D006038:Glyoxylates; D006651:Histocytochemistry / Q000379:methods; D008833:Microcirculation
chemical_list: D006038:Glyoxylates; C031150:glyoxylic acid