[The effect of caffeine on the electrical reactions of the acinar cells in the canine pancreas].
The action of pharmacological drugs (caffeine, procaine, ruthenium red) which influenced the release of Ca2+ from endoplasmatic reticulum, the electrical responses of dog pancreatic acinar cells was investigated using intracellular glass microelectrodes. These drugs were used to elucidate the mechanisms of Ca2+ release from endoplasmatic reticulum. Membrane depolarization and decrease of input resistance were observed in the presence of caffeine. Procaine and ruthenium red suppressed electrical responses of acinar cells to caffeine. The results obtained permit supposing that there are two mechanisms of Ca2+ release from intracellular stores in pancreatic acinar cells: Ca(2+)-induced one and using inositol-1,4,5-triphosphate.
Shevchuk
P M
PM
Mahura
I S
IS
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology; D002217:Carbachol / Q000494:pharmacology; D004285:Dogs; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008839:Microelectrodes; D010179:Pancreas / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010418:Pentagastrin / Q000494:pharmacology
D002110:Caffeine; D002217:Carbachol; D010418:Pentagastrin; D000109:Acetylcholine; D002118:Calcium
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