The effect of atherogenic infusions of the triglyceride-rich, lipid emulsion, Lipofundin-S, on the in vitro growth characteristics of rat aortic smooth muscle cells.
This study shows that arterial smooth muscle cells (SMC) isolated from rats receiving atherogenic doses of the lipid emulsion, Lipofundin-S, alter their in vitro growth properties. Compared to cells from control animals, SMC isolated from Lipofundin-S-infused rats show a reduction in both saturation density and response to increasing serum concentrations, without a change in the baseline proliferation. Also, SMC isolated from lipid-treated animals and grown for five days in the presence of 30, 150, or 300 pg/ml estradiol show a 30% increase in growth vs. cells from controls. Epinephrine at 1 microM stimulates growth in SMC from control rats, while causing no growth enhancement over five days in cells from lipid-infused animals. Thus, atherogenic infusions of Lipofundin-S into rats cause phenotypic changes in arterial SMC which can be passed to successive cell generations in vitro.
Saladino
C F
CF
Department of Medicine, Nassau County Medical Center, East Meadow, New York 11554.
Yeh
Q
Q
Zimmer
E
E
Jonas
E A
EA
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000187:drug effects; D001161:Arteriosclerosis / Q000139:chemically induced*; D001790:Blood Physiological Phenomena; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004338:Drug Combinations; D004837:Epinephrine / Q000502:physiology; D004958:Estradiol / Q000502:physiology; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage* / Q000737:chemistry; D005990:Glycerol / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D010743:Phospholipids / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D013024:Soybean Oil / Q000008:administration & dosage* / Q000737:chemistry; D014280:Triglycerides / Q000032:analysis*
D004338:Drug Combinations; D005217:Fat Emulsions, Intravenous; D010743:Phospholipids; D014280:Triglycerides; D004958:Estradiol; C046535:Lipofundin S; D013024:Soybean Oil; D005990:Glycerol; D004837:Epinephrine