Evaluation of metastatic human tumor burden and response to therapy in a nude mouse xenograft model using a molecular probe for repetitive human DNA sequences.
A sensitive DNA dot-blot assay for repetitive human DNA sequences was developed and applied to the quantitative determination of spontaneous metastases of a human melanoma in various tissues of nude mice. The assay was useful for defining the time course and pattern of tissue distribution of metastatic cells as well as for assessing response to therapy. The methodology is relatively simple, can be performed using nonradioactive DNA probes, and should be broadly applicable to studies of metastasis of human tumors in nude mice.
Shoemaker
R H
RH
Laboratory of Drug Discovery Research and Development, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702.
Smythe
A M
AM
Wu
L
L
Balaschak
M S
MS
Boyd
M R
MR
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics*; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008175:Lung Neoplasms / Q000188:drug therapy / Q000556:secondary; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics* / Q000473:pathology; D009368:Neoplasm Transplantation; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D004273:DNA, Neoplasm; D003520:Cyclophosphamide; D004247:DNA