title: The impenetrability of 5-(N-hexadecanoyl)aminofluoroscein through endothelial cell monolayers is dependent upon its solution properties, not the presence of tight junctions.
abstract: The solution properties of two fluorescent lipophilic analogues were examined in conjunction with their ability to penetrate the tight junctions of bovine aortic endothelial cell monolayers. 5-(N-dodecanoyl)aminofluoroscein was shown to label both the apical and basolateral plasma membrane domains of confluent monolayers at 4 degrees C and pH 7.3, but 5-(N-hexadecanoyl)aminofluoroscein was shown to label only the apical membrane domain. When used under more soluble conditions at 20 degrees C and pH 8.5, both probes labeled apical and basolateral plasma membrane domains more equally. This indicates that solubility conditions, and not tight junctions, dictate the penetration of 5-(N-hexadecanoyl)aminofluoroscein from the apical to the basolateral plasma membrane domain.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003.
authors.forename: D B
authors.initials: DB
authors.lastname: Stolz
authors.forename: M G
authors.initials: MG
authors.lastname: Mahoney
authors.forename: B S
authors.initials: BS
authors.lastname: Jacobson
mesh_terms: D000818:Animals; D001011:Aorta; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D005452:Fluoresceins / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes; D007365:Intercellular Junctions / Q000378:metabolism*; D007700:Kinetics; D008856:Microscopy, Fluorescence; D012995:Solubility; D013696:Temperature
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; C074109:5-(N-dodecanoyl)aminofluorescein; C058719:(hexadecanoyl)aminofluorescein