title: Mesenchyme-mediated effect of dexamethasone on laminin in cocultures of embryonic gut epithelial cells and mesenchyme-derived cells.
abstract: Previous studies have shown that glucocorticoids accelerate intestinal maturation and that this process is mediated by the mesenchymal cells. The possible involvement of laminin (LN), a basement membrane component, in this mesenchymal mediation has been analyzed. For this purpose, the influence of dexamethasone (DX) on the synthesis of LN, its chain composition and its cellular distribution has been examined biochemically and immunocytochemically in two different mesenchyme-derived cell populations, fetal intestinal mesenchymal cells and fetal skin fibroblasts, as well as in cocultures of intestinal endodermal cells seeded on top of confluent fetal skin fibroblasts. Neither the amount of metabolically labeled LN purified by affinity chromatography (expressed per mg cell proteins), nor the A versus B chain ratio monitored after separation on gel electrophoresis and immunoblotting, showed significant differences after 5 days of DX treatment. However, glucocorticoids induced a shift from secreted to cell-associated LN molecules paralleling a striking difference in the immunostaining pattern of intracellular and surface LN in the mesenchyme-derived cell monocultures; the granular intracytoplasmic LN staining in the control cultures was replaced by a fibrillar organization of LN molecules concomitantly with an increased accumulation at the cell surface. In 2-day DX-treated cocultures, there was an acceleration of LN deposition at the epithelial-fibroblastic interface, which accompanied the enhanced expression of epithelial cell differentiation markers (brush border digestive enzymes). These DX-induced changes can be blocked by the addition of anti-LN antibodies in the culture medium. These findings further support the concept that glucocorticoid action on intestinal epithelial cells involves alterations in the extracellular microenvironment, assessed here for LN molecules, occurring at the level of the mesenchymal cell compartment. These changes may contribute to an accelerated organization of LN at the epithelial-mesenchymal interface and subsequently to epithelial differentiation.
authors.affiliation: INSERM Unité 61, Biologie Cellulaire et Physiopathologie Digestives, Strasbourg, France.
authors.forename: P
authors.initials: P
authors.lastname: Simo
authors.forename: P
authors.initials: P
authors.lastname: Simon-Assmann
authors.forename: C
authors.initials: C
authors.lastname: Arnold
authors.forename: M
authors.initials: M
authors.lastname: Kedinger
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D004707:Endoderm / Q000166:cytology / Q000187:drug effects*; D007422:Intestines / Q000166:cytology / Q000196:embryology; D007797:Laminin / Q000032:analysis / Q000037:antagonists & inhibitors*; D008648:Mesoderm / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000166:cytology
chemical_list: D007797:Laminin; D003907:Dexamethasone