title: Microscale production of hybridomas by hypo-osmolar electrofusion.
abstract: We have developed and tested a novel electrofusion chamber, the adjustable plate microchamber, that permits the successful electrofusion and production of hybridomas in a hanging droplet from as few as 1,000 B lymphocytes. Cell suspension volumes of 10 microliters may be used without excessive difficulty in aseptically recovering fused cells. With a modification of the hypo-osmolar electrofusion protocol with this microchamber, fusion efficiencies of the order of 10(-3) may be attained. These efficiencies are comparable to those attained with standard hardware and much higher numbers of input B lymphocytes. This technology should permit high efficiency hybridoma production using a variety of hitherto inaccessible B lymphocyte populations such as B cells isolated from human organ biopsies.
authors.affiliation: Lehrstuhl für Biotechnologie, Universität Würzburg, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Zimmermann
authors.forename: G
authors.initials: G
authors.lastname: Klöck
authors.forename: P
authors.initials: P
authors.lastname: Gessner
authors.forename: D W
authors.initials: DW
authors.lastname: Sammons
authors.forename: G A
authors.initials: GA
authors.lastname: Neil
mesh_terms: D000818:Animals; D001402:B-Lymphocytes; D002459:Cell Fusion; D002478:Cells, Cultured; D015194:Diffusion Chambers, Culture; D005260:Female; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C