An autocrine mitogenic activity produced by a pleural human mesothelioma cell line.
The pleural human mesothelioma cell line ZL5 established in our laboratory exhibits an unusual phenotype with adherent and floating cells. ZL5 cells grow in a chemically defined medium (ACL3*) and can be maintained over 3 weeks in protein-free basal medium alone (RPMI). Basal medium conditioned by ZL5 cells possesses a mitogenic activity with an autocrine effect, as measured by cell counting and by a 3H-thymidine incorporation assay. Moreover, the conditioned medium affects the DNA synthesis of a variety of other lung-derived cells. The active principle of medium conditioned by ZL5 cells is not identical to the defined growth-factors EGF, PDGF, and TGF-beta, known to stimulate the growth of normal human mesothelial cells: treatment with these factors does not mimic the effect of conditioned medium on ZL5 cells. Our observations suggest that the mesothelioma cell line ZL5 produces an unknown autocrine mitogen.
Lauber
B
B
Department of Medicine, University Hospital, CH-8091 Zurich, Switzerland.
Leuthold
M
M
Schmitter
D
D
Cano-Santos
J
J
Waibel
R
R
Stahel
R A
RA
D000230:Adenocarcinoma; D000328:Adult; D000818:Animals; D002294:Carcinoma, Squamous Cell; D002455:Cell Division; D002460:Cell Line; D002871:Chromosome Banding; D003470:Culture Media; D004261:DNA Replication; D006801:Humans; D007621:Karyotyping; D008168:Lung; D008297:Male; D008654:Mesothelioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008934:Mitogens / Q000302:isolation & purification / Q000378:metabolism*; D009368:Neoplasm Transplantation; D010996:Pleural Effusion / Q000473:pathology; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D003470:Culture Media; D008934:Mitogens