Somatic cell mapping of bovine EC-SOD and SOD1L loci.
cDNA probes of human extracellular superoxide dismutase (EC-SOD) and bovine superoxide dismutase 1 (SOD1) genes were hybridized to Southern blots containing genomic DNAs from cow-rodent somatic cell lines segregating bovine chromosomes. The SOD1 probe identified two loci: the coding locus (SOD1), which mapped to bovine U10; and a related locus (SOD1L), which mapped to U11. EC-SOD mapped to bovine U15. The mapping of EC-SOD to human chromosome 4, and our mapping of EC-SOD to U15, further defines a region of extensive syntenic conservation between humans and domestic cows.
Gallagher
D S
DS
Department of Veterinary Pathobiology, Texas A & M University, College Station 77843-4463.
Gibbs
L S
LS
Shaffer
J B
JB
Womack
J E
JE
D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D002460:Cell Line; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D006822:Hybrid Cells; D013482:Superoxide Dismutase / Q000235:genetics*
D004247:DNA; D013482:Superoxide Dismutase