Ultrastructural localization of calcium in ischemic hippocampal slices: the influence of adenosine and theophylline.
Calcium was localized ultrastructurally with the use of the modified oxalate-pyroantimonate reaction in the CA1 region of rat hippocampal slices. Ten-minute ischemia (incubation with anoxic and glucose-free medium) followed by 30 min reoxygenation resulted in mitochondrial calcium sequestration and ultrastructural damage. The addition of the adenosine receptor antagonist, theophylline, worsened the ischemia-induced morphological changes and particularly exaggerated the Ca2+ loading in the postsynaptic dendrites. In contrast, adenosine protected against ischemia-induced changes. The results suggest that adenosine exerts its neuroprotective action largely by maintaining intracellular calcium-homeostasis.
Dux
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Max-Planck Institute of Psychiatry, Department of Neuromorphology, Martinsried, Germany.
Schubert
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P
Kreutzberg
G W
GW
D000241:Adenosine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000517:prevention & control; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D003712:Dendrites / Q000737:chemistry; D006624:Hippocampus / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D008928:Mitochondria / Q000737:chemistry; D009924:Organ Culture Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D013806:Theophylline / Q000494:pharmacology*
D013806:Theophylline; D000241:Adenosine; D002118:Calcium