Effect of electric charged molecules on Sindbis virus hemagglutination and hemolysis.
The role of electrostatic interactions in the attachment and fusion at acidic pH of Sindbis virus (SNV) with goose erythrocytes was studied, investigating the effect of several anionic and cationic polyelectrolytes on SNV hemagglutination and hemolysis. In order to establish the target of active drugs, the compounds were incubated either with the virus particles or with the erythrocytes. Dextran sulfate was the only compound able to inhibit the attachment of SNV to the erythrocytes. Fusion of virus with red cells was reduced dose-dependently by the polyanions dextran sulfate, mucin and polygalacturonic acid. On the contrary two polycations, polylysine and polybrene, enhanced viral hemolytic activity. However the effect of polyions is not exclusively related to the electric charge since ineffective molecules were found in both classes of compounds.
Mastromarino
P
P
Institute of Microbiology, School of Medicine, University of Rome La Sapienza, Italy.
Conti
C
C
Petruzziello
C
C
Lapadula
R
R
Orsi
N
N
D000818:Animals; D002809:Chondroitin Sulfates / Q000494:pharmacology; D016264:Dextran Sulfate / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005777:Geese / Q000097:blood; D006387:Hemagglutination, Viral / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D006493:Heparin / Q000494:pharmacology; D006583:Hexadimethrine Bromide / Q000494:pharmacology; D006657:Histones / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009077:Mucins / Q000494:pharmacology; D010368:Pectins / Q000494:pharmacology; D011107:Polylysine / Q000494:pharmacology; D011112:Polymyxin B / Q000494:pharmacology; D011479:Protamines / Q000494:pharmacology; D012845:Sindbis Virus / Q000187:drug effects* / Q000378:metabolism; D014709:Vero Cells
D006657:Histones; D009077:Mucins; D011479:Protamines; D011107:Polylysine; D006583:Hexadimethrine Bromide; D010368:Pectins; D006493:Heparin; D002809:Chondroitin Sulfates; D016264:Dextran Sulfate; D011112:Polymyxin B; C003181:polygalacturonic acid