Friend murine leukaemia virus is integrated at a common site in most primary spleen tumours of erythroleukaemic animals.
A common proviral integration site was identified and characterized in erythroleukaemias induced by Friend murine leukaemia virus (F-MuLV). Using inverse polymerase chain reaction, we found a proviral integration site common to at least 90% of 20 primary tumours tested. This site was identical to Fli-1, a locus recently reported by others to be rearranged in 75% (9/12) of cell lines established from spleens of erythroleukaemic mice and to code for a member of the ets gene family. Our data suggest that about half of the F-MuLV-induced erythroleukaemias contained more than one cell clone with a proviral integration in Fli-1, with different individual integration sites within Fli-1 in each cell clone. All proviruses were found to be integrated in the same transcriptional orientation with respect to flanking cellular DNA. We discuss these findings in relation to multistage models of neoplasm.
Sels
F T
FT
Institute of Medical Virology, Justus Liebig University, Giessen, Germany.
Langer
S
S
Schulz
A S
AS
Silver
J
J
Sitbon
M
M
Friedrich
R W
RW
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D005622:Friend murine leukemia virus / Q000235:genetics* / Q000472:pathogenicity; D015967:Gene Expression Regulation, Viral; D004915:Leukemia, Erythroblastic, Acute / Q000382:microbiology*; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000235:genetics; D015183:Restriction Mapping; D013160:Splenic Neoplasms / Q000382:microbiology*; D014158:Transcription, Genetic; D016662:Virus Integration
D004273:DNA, Neoplasm; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D012334:RNA, Neoplasm