Construction of chimeric antibodies: cloning of immunoglobulin genes including their promoter regions by PCR.
In the production of recombinant antibodies, it is necessary to have an immunoglobulin gene promoter for driving the expression of the antibody genes. Here we describe a simple PCR method that allows cloning of the immunoglobulin genes together with their own promoters despite the fact that the sequence of the upstream part of the gene is unknown.
Mocikat
R
R
GSF-Institut für Immunologie, München, FRG.
Kütemeier
G
G
Harloff
C
C
D000818:Animals; D000906:Antibodies / Q000235:genetics*; D001483:Base Sequence; D001709:Biotechnology; D002678:Chimera; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005803:Genes, Immunoglobulin; D006825:Hybridomas / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic
D000906:Antibodies; D004247:DNA