title: [Studies of pepsinogen release in guinea pig gastric chief cells based on changes of cytosolic free Ca2+ concentration].
abstract: In order to evaluate the role of secretagogue-induced changes of cytosolic free calcium concentration ([Ca2+]i) in pepsinogen release, the effects of cholecystokinin-octapeptide (CCK-8) on pepsinogen release and on [Ca2+]i in gastric chief cells isolated from guinea pig stomach were investigated. [Ca2+]i was measured with fura-2, Ca(2+)-sensitive fluorescent probe, using dual-wavelength excitation fluorospectrophotometer. In the presence of extracellular Ca2+, CCK-8 induced a rapid and transient increase in [Ca2+]i followed by a sustained plateau of [Ca2+]i increase which remained 20-50 nM above basal level until antagonist was added. Both in the absence of external Ca2+ and in the presence of inorganic Ca2+ channel blocker Ni2+(2 nM), plateau phase but not initial transient peak of [Ca2+]i was diminished. On the other hand, nicardipine, an organic Ca2+ channel blocker, did not affect the [Ca2+]i transient induced by CCK-8. Time course of CCK-8-stimulated pepsinogen release was biphasic; the initial phase of pepsinogen release was unaffected by Ca2+ removal from the medium but was inhibited by Ca2+ chelation with an intracellular Ca2+ chelator BAPTA/AM, while the late phase of pepsinogen release was dependent on extracellular Ca2+. Artificially introduced Ca2+ into the chief cells with ionomycin elicited the increase in [Ca2+]i and pepsinogen release. However, ionomycin-induced release of pepsinogen was transient and several times smaller than that induced by CCK-8. Ni(2+)-sensitive Ca2+ influx across the plasma membrane was also necessary for reloading of agonist-sensitive Ca2+ pool. These results suggest that the initial, transient increase in [Ca2+]i from the intracellular Ca2+ store mediates the initial pepsinogen release, while the sustained increase in [Ca2+]i which is dependent on Ca2+ influx is responsible for the late phase of pepsinogen release. Factors other than the changes in [Ca2+]i may also be required in producing optimal pepsinogen release.
authors.affiliation: Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takeda
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D005753:Gastric Mucosa / Q000378:metabolism*; D006168:Guinea Pigs; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D010435:Pepsinogens / Q000378:metabolism*
chemical_list: D002121:Calcium Channel Blockers; D002614:Chelating Agents; D007476:Ionophores; D010435:Pepsinogens; D002118:Calcium