Expression of recombinant platelet-derived endothelial cell growth factor in the yeast Saccharomyces cerevisiae.
A platelet-derived endothelial cell growth factor cDNA has been cloned, sequenced and expressed using the Saccharomyces cerevisiae PRB1 promoter. Soluble recombinant platelet-derived endothelial cell growth factor constituted 0.5-1.0% of total soluble protein. Yeast soluble protein extracts containing recombinant platelet-derived endothelial cell growth factor stimulate the growth of calf pulmonary artery endothelial cells in vitro.
Finnis
C
C
Delta Biotechnology Ltd, Nottingham, U.K.
Goodey
A
A
Courtney
M
M
Sleep
D
D
D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D004730:Endothelium, Vascular / Q000187:drug effects; D006801:Humans; D011651:Pulmonary Artery / Q000187:drug effects; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013939:Thymidine Phosphorylase / Q000096:biosynthesis* / Q000494:pharmacology
D011994:Recombinant Proteins; D013939:Thymidine Phosphorylase