Transcription initiation and temporal expression of thymidine kinase mRNA in Chinese hamster cells.
The induction of thymidine kinase mRNA has proved to be a valuable model for understanding regulatory events at the G1/S boundary of the cell cycle (1, 2, 3). As an initial step toward characterizing the regulation of this gene in Chinese hamster cells, I have mapped the transcription start sites for TK mRNA in CHEF/18 cells. Two closely spaced sites of transcription initiation were detected downstream of a nonconsensus TATAA element in the promoter region. Using primer extension analyses, I demonstrated that the transcription initiation sites remained constant while the absolute levels of TK mRNA varied during the cell cycle in synchronized populations of Chinese hamster cells.
Gudas
J M
JM
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA.
D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016385:TATA Box; D013937:Thymidine Kinase / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D013937:Thymidine Kinase