Neoplastic expression in murine cells induced by halogenated hydrocarbons.
The neoplastic expression in mouse embryo fibroblasts exposed to 1,2-dibromoethane and its chloroanalogue, 1,2-dichloroethane in vitro, was examined. Both substances are widely used as fumigants for carpet and upholstery, as gasoline additives, and as organic solvents. Both are known to be highly toxic, mutagenic, and carcinogenic agents. C3H10T1/2 cells treated with these haloalkanes exhibited altered morphology and were selected further by cloning in soft agar. Soft agar clones were found to induce a 100% multitumor occurrence in the nude mouse model. These results suggest that this pair of mutagens have altered the normal phenotype of mouse embryo cells, and these cells have become neoplastic. These neoplastic cell lines will be useful as an in vitro model to study the role of genetic changes in the transformation processes induced by halogenated hydrocarbons.
Schultz
K
K
Department of Molecular Biology, Research Institute, Cleveland Clinic Foundation, Ohio 44195.
Ghosh
L
L
Banerjee
S
S
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015946:Ethylene Dibromide / Q000008:administration & dosage / Q000494:pharmacology*; D005025:Ethylene Dichlorides / Q000008:administration & dosage / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D010641:Phenotype
D002273:Carcinogens; D005025:Ethylene Dichlorides; D015946:Ethylene Dibromide; C024565:ethylene dichloride