Immunocytochemical and ultrastructural studies of eosinophilic granular bodies in astrocytic tumors.
Eosinophilic granular bodies (EGBs) are studied immunocytochemically and ultrastructurally in a case of low-grade and a case of high-grade astrocytoma. EGBs are recognized as brightly eosinophilic round bodies of variable size in hematoxylin and eosin-stained sections. Immunocytochemically some EGBs are positive for antibodies raised against alpha B-crystallin, ubiquitin and glial fibrillary acidic protein with the staining patterns for each being different from one another. Ultrastructurally EGBs consist of membrane-bound round body of various diameter ranging from 50 nm to 20 microns. Small EGBs contain electron-dense homogeneous material with occasional myelin figures, while electron-dense homogeneous material or loose granular profiles. Our studies demonstrate (1) ultrastructural variety of EGB; (2) and alpha B-crystallin epitope in EGB; and (3) the presence of EGB in high-grade as well as low-grade astrocytoma.
Murayama
S
S
Department of Pathology, University of North Carolina, Chapel Hill 27599-7525.
Bouldin
T W
TW
Suzuki
K
K
D000328:Adult; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001254:Astrocytoma / Q000473:pathology* / Q000648:ultrastructure; D001932:Brain Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D002648:Child; D003459:Crystallins / Q000032:analysis; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004804:Eosinophils / Q000473:pathology* / Q000648:ultrastructure; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron
D000906:Antibodies; D000911:Antibodies, Monoclonal; D003459:Crystallins; D005904:Glial Fibrillary Acidic Protein