title: Evaluation of enzyme-linked immunosorbent assay and counter-current immunoelectrophoresis in diagnosis of experimental trichinosis.
abstract: Definite diagnosis of trichinosisis mainly by direct demonstration of larvae, however, these methods are neither feasible nor sensitive. Thus, confirmation relies mainly on positive serology. In this study, enzyme-linked immunosorbent assay (ELISA) and counter-current immunoelectrophoresis (CIEP) using crude larval antigen were assessed as a tool in the diagnosis of trichinosis. Sera were tested one, two, five, eight, and twelve weeks post-infection. It was found that ELISA was positive in all tested sera from the second till the 12th week (100% sensitivity). As regards CIEF, 36% of the sera were positive two weeks post-infection, while all sera were positive at 5 and 8 weeks. Ninety per cent of the tested sera gave positive results at the 12th week post-infection. ELISA proved to be more sensitive in diagnosing trichinosis both early and late in infection, while CIEP test was more specific since no false positive results were noticed.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Alexandria, Egypt.
authors.forename: M M
authors.initials: MM
authors.lastname: el-Temsahi
authors.forename: L M
authors.initials: LM
authors.lastname: Abu-Samra
authors.forename: S T
authors.initials: ST
authors.lastname: el-Mansoury
authors.forename: R M
authors.initials: RM
authors.lastname: Barakat
authors.forename: H N
authors.initials: HN
authors.lastname: Awadalla
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D003378:Counterimmunoelectrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D012680:Sensitivity and Specificity; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis*
chemical_list: D000909:Antibodies, Helminth