title: Steroid sulfatase in the human ovary and placenta: enzyme kinetics and phosphate inhibition.
abstract: In 5 placental homogenates the Km of steroid sulfatase for DHEA sulfate increased from 15.4 in Tris buffer to 26.8 microM in phosphate (both buffers 0.1 M, pH 7.4), P less than 0.05. In 3 pooled ovarian preparations the Km increased from 14.3 microM in Tris to 33.0 microM in phosphate, P less than 0.01. There was no significant difference between the ovarian and placental values for Km in either Tris or phosphate (P greater than 0.5), and the increase in the Km produced by phosphate in ovarian tissue was not significantly different from that in the placenta (P greater than 0.5). In the placentas the Vmax in Tris was 1420 pmol/min/mg protein and this fell to 523 pmol/min/mg protein in phosphate (P less than 0.005). The Vmax was 50-fold higher in the placenta than in the ovary in either Tris or phosphate (both P less than 0.001). In the ovary, the Vmax was 27.6 pmol/min/mg protein in Tris and 11.0 pmol/min/mg protein in phosphate (P less than 0.05). The reduction of Vmax produced by phosphate in the ovary was not significantly different from that in the placenta (P greater than 0.5). The slope of the 1/v vs 1/S plot (Km/Vmax) increased 4.7-fold in the placentas and 5.8-fold in the ovaries in phosphate over that in Tris (both P less than 0.001); the increase in the placentas was not significantly different from that in the ovaries (P greater than 0.5). Phosphate ion acts as a mixed inhibitor of both placental and ovarian steroid sulfatase.
authors.affiliation: Department of Obstetrics and Gynecology, Brown University, Providence, RI.
authors.forename: R V
authors.initials: RV
authors.lastname: Haning
authors.forename: R J
authors.initials: RJ
authors.lastname: Hackett
authors.forename: J A
authors.initials: JA
authors.lastname: Canick
mesh_terms: D001192:Arylsulfatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005260:Female; D006801:Humans; D007700:Kinetics; D010053:Ovary / Q000201:enzymology*; D010710:Phosphates / Q000378:metabolism; D010920:Placenta / Q000201:enzymology*; D043266:Steryl-Sulfatase
chemical_list: D010710:Phosphates; D001192:Arylsulfatases; D043266:Steryl-Sulfatase