title: Infection of owl monkeys (Aotus trivirgatus) and cynomolgus monkeys (Macaca fascicularis) with hepatitis E virus from Mexico.
abstract: Owl and cynomolgus monkeys were inoculated with hepatitis E virus (HEV) to compare disease models and produce antibody and virus. By immune electron microscopy (IEM), all six owl monkeys were shown to have serologic responses manifested by unusually high levels of anti-HEV at 6 months, but only three developed hepatitis. Virus-related antigen in liver (HEV Ag) was detected by immunofluorescence microscopy of biopsies from two of four owl monkeys; one with HEV Ag also had HEV in acute-phase bile (detected by IEM) and feces (detected by infecting another owl monkey). In contrast, cynomolgus monkeys propagated HEV to higher levels and all five had hepatitis. Moderate-to-high levels of HEV Ag correlated with detectable HEV in bile for both species. Thus, the value of using HEV-infected cynomolgus was confirmed. Owl monkeys were shown to be HEV-susceptible and sources of high-level anti-HEV; Sustained anti-HEV in these monkeys may also be useful for understanding immune responses.
authors.affiliation: Department of Viral Diseases, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
authors.forename: J
authors.initials: J
authors.lastname: Ticehurst
authors.forename: L L
authors.initials: LL
authors.lastname: Rhodes
authors.forename: K
authors.initials: K
authors.lastname: Krawczynski
authors.forename: L V
authors.initials: LV
authors.lastname: Asher
authors.forename: W F
authors.initials: WF
authors.lastname: Engler
authors.forename: T L
authors.initials: TL
authors.lastname: Mensing
authors.forename: J D
authors.initials: JD
authors.lastname: Caudill
authors.forename: M H
authors.initials: MH
authors.lastname: Sjogren
authors.forename: C H
authors.initials: CH
authors.lastname: Hoke
authors.forename: J W
authors.initials: JW
authors.lastname: LeDuc
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001029:Aotus trivirgatus; D001646:Bile / Q000382:microbiology; D004195:Disease Models, Animal; D005243:Feces / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006508:Hepatitis Antibodies / Q000096:biosynthesis / Q000097:blood; D016751:Hepatitis E / Q000276:immunology* / Q000382:microbiology; D016752:Hepatitis E virus / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008252:Macaca fascicularis; D008800:Mexico; D016253:Microscopy, Immunoelectron; D014771:Virion / Q000648:ultrastructure; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D006508:Hepatitis Antibodies; D000410:Alanine Transaminase