Inhibition of interleukin 1 induced chondrocyte protease activity by a corticosteroid and a nonsteroidal antiinflammatory drug.
We report that administration of the corticosteroid, methylprednisolone (PRED) inhibited interleukin 1 (IL-1) induction of chondrocyte caseinolytic activity (25-55%) and collagenolytic activity (15-24%). The nonsteroidal antiinflammatory drug (NSAID), naproxen (NAP) had no effect on either enzyme activity over a therapeutic range (7-30 micrograms/ml) but at 120 micrograms/ml inhibited IL-1 induced caseinolytic and collagenolytic activity by 17 and 19%, respectively. However, PRED (2 micrograms/ml) in combination with NAP (30 micrograms/ml) significantly increased the inhibition of caseinolytic activity (p less than 0.001) compared to that observed with PRED (2 micrograms/ml) alone. The suppression of IL-1 induced collagenolytic activity noted with PRED in combination with NAP did not exceed that observed with PRED alone.
Lane
N E
NE
Division of Rheumatology, UCSF.
Williams
R J
RJ
Schurman
D J
DJ
Smith
R L
RL
D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000166:cytology / Q000201:enzymology*; D002364:Caseins / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology*; D008775:Methylprednisolone / Q000494:pharmacology; D009288:Naproxen / Q000494:pharmacology; D011458:Prostaglandins E / Q000097:blood; D011480:Protease Inhibitors / Q000494:pharmacology*
D000305:Adrenal Cortex Hormones; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002364:Caseins; D007375:Interleukin-1; D011458:Prostaglandins E; D011480:Protease Inhibitors; D009288:Naproxen; D003094:Collagen; D010450:Endopeptidases; D008775:Methylprednisolone