The wavelength of light governing intraocular immune reactions.
Injection of antigen into the anterior chamber of the eye results in the induction of suppressed systemic cell-mediated responses as measured by delayed-type hypersensitivity or contact hypersensitivity (CHS). Previous studies from the author's laboratories have determined that this response is governed by exposure of the eye to visible light during the initial intraocular encounter between T cells and antigen. To more fully understand the role of light, as well as to begin to understand the molecular mediators involved, the authors chose to explore the properties of light governing the effect. Neutral density filter were used to demonstrate that the minimum amount of light required to induce suppression of CHS following anterior chamber injection of antigen is 1-2 lux (lumens/meter2). With narrow band filters, the wavelengths responsible for suppression were shown to be 500-510 nm. The results show that the effect of light extends beyond the hapten-derivatized spleen cell system to other antigens placed in the anterior chamber of the eye. Studies also show that the retina and the pineal gland, two light absorbing structures, may not be involved. The results in this report show that light of very restricted wavelengths controls intraocular immune reactions.
Ferguson
T A
TA
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, Missouri.
Mahendra
S L
SL
Hooper
P
P
Kaplan
H J
HJ
D000818:Animals; D000867:Anterior Chamber / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology; D005123:Eye / Q000276:immunology* / Q000528:radiation effects; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007109:Immunity / Q000528:radiation effects; D008027:Light; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D010870:Pineal Gland / Q000276:immunology; D012160:Retina / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology