Human T-lymphocyte proliferation, lymphokine production, and amebicidal activity elicited by the galactose-inhibitable adherence protein of Entamoeba histolytica.
We studied human T-lymphocyte responses to the purified Entamoeba histolytica galactose-inhibitable adherence protein. Individuals having serum anti-adherence protein antibodies possess peripheral blood lymphocytes which demonstrate antigen-specific responses to the purified adherence protein (10 micrograms/ml) and whole soluble amebic antigen (100 micrograms/ml). This was determined by incorporation of [3H]thymidine (53,080 and 73,114 dpm, respectively) and by increased production of interleukin-2 and gamma interferon (42.0 and 67.5 U/ml, respectively) (P less than 0.05 for each in comparison with values for control lymphocyte responses). Lymphocytes from antiamebic antibody-positive subjects develop in vitro amebicidal activity only when incubated for 5 days with the purified adherence protein (P = 0.02). In conclusion, the E. histolytica galactose-inhibitable adherence protein elicits an in vitro amebicidal cell-mediated immune response, further supporting the potential for the use of this protein in a subunit amebiasis vaccine.
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Department of Medicine, University of Virginia School of Medicine, Charlottesville 22908.
Salata
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Ravdin
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D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004748:Entamoeba histolytica / Q000276:immunology*; D005690:Galactose / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*; D015800:Protozoan Proteins / Q000494:pharmacology*; D016052:Protozoan Vaccines / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D008222:Lymphokines; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D005690:Galactose