title: Fetal-type phosphorylation of the tau in paired helical filaments.
abstract: To determine the phosphorylation sites of the tau in paired helical filaments (PHF), two types of PHF antisera with different specificities were used: One was a conventional anti-PHF, and the other was an antiserum to formic acid-denatured PHF (anti-HFoPHF). Phosphorylated tau-specific antibodies, anti-ptau 1 and anti-ptau 2, were prepared from anti-PHF and anti-HFoPHF, respectively. We found that both anti-ptau 1 and anti-ptau 2 labeled fetal or juvenile tau but not adult tau. The anti-ptau 1- and anti-ptau 2-recognition sites were immunochemically localized to the fragment Asp313 to Ile328 in the most COOH-terminal portion of tau. Furthermore, Ser315 was determined as the anti-ptau 2 recognition site. The sequence surrounding Ser315 was not found in the canonical sequences phosphorylated with known kinases.
authors.affiliation: Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kanemaru
authors.forename: K
authors.initials: K
authors.lastname: Takio
authors.forename: R
authors.initials: R
authors.lastname: Miura
authors.forename: K
authors.initials: K
authors.lastname: Titani
authors.forename: Y
authors.initials: Y
authors.lastname: Ihara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D016874:Neurofibrillary Tangles / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D010766:Phosphorylation; D051381:Rats; D016875:tau Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000906:Antibodies; D007106:Immune Sera; D016875:tau Proteins