Gas2, a growth arrest-specific protein, is a component of the microfilament network system.
In this report we analyze the protein product of a growth arrest-specific gene, gas2, by means of an affinity-purified antibody raised against the protein produced in bacteria. The regulation of Gas2 biosynthesis reflects the pattern of mRNA expression (Schneider, C., R. King, and L. Philipson. 1988. Cell. 54:787-793): its relative level is tightly associated with growth arrest. Gas2 seems to be regulated also at the posttranslational level via a phosphorylation mechanism. Gas2 is well conserved during the evolution with the same apparent molecular mass (36 kD) between mouse and human. We also demonstrate that Gas2 is a component of the microfilament system. It colocalizes with actin fiber, at the cell border and also along the stress fiber, in growth-arrested NIH 3T3 cells. The pattern of distribution, detected in arrested cells, can also be observed in growing cells when they are microinjected with the purified GST-Gas2 protein. In none of the analyzed oncogene-transformed NIH 3T3 cell lines was Gas2 expression induced under serum starvation.
Brancolini
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C
International Center for Genetic Engineering and Biotechnology (I.C.G.E.B.) Trieste, Italy.
Bottega
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S
Schneider
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C
D016475:3T3 Cells; D000818:Animals; D015153:Blotting, Western; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D004926:Escherichia coli / Q000235:genetics; D016193:G1 Phase; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006131:Growth Inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008845:Microinjections; D008856:Microscopy, Fluorescence; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016192:Resting Phase, Cell Cycle
C490934:GAS2L1 protein, human; D006131:Growth Inhibitors; D008565:Membrane Proteins; D008840:Microfilament Proteins; D011993:Recombinant Fusion Proteins; C031717:growth inhibitory proteins; D005982:Glutathione Transferase