Melanins production from enkephalins by tyrosinase.
Leu-enkephalin and Met-enkephalin are oxidized in vitro by mushroom and sepia tyrosinase giving rise to synthetic melanins whose production is dependent on incubation time and on enzyme concentration. The Enk-melanins formed are acid-insoluble brownish or reddish pigments showing a continuous absorbance in the visible region when dissolved in basic solution. The presence of the amino acid chain makes them fully soluble in pH 7.4 0.05 M phosphate buffer and methanol.
Rosei
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Dipartimento di Scienze Biochimiche, Università La Sapienza, Rome, Italy.
Mosca
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De Marco
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D000596:Amino Acids / Q000032:analysis; D001487:Basidiomycota / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D004743:Enkephalin, Leucine / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000378:metabolism*; D007700:Kinetics; D008543:Melanins / Q000302:isolation & purification / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D013053:Spectrophotometry; D013379:Substrate Specificity
D000596:Amino Acids; D008543:Melanins; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D014442:Monophenol Monooxygenase