title: Conjugal transfer of plasmid DNA from Enterococcus faecalis to Escherichia coli in digestive tracts of gnotobiotic mice.
abstract: We have studied the transfer of the conjugative shuttle plasmid pAT191, which confers resistance to kanamycin, from Enterococcus faecalis to Escherichia coli in the digestive tracts of six gnotobiotic mice. Colonies of E. coli resistant to kanamycin were isolated from the feces of two mice, respectively, on days 25 and 35 after the beginning of the experiment and never thereafter. The transfer frequency of pAT191, expressed as the number of transconjugants per donor cell isolated from intestines of sacrificed mice, was 3 x 10(-9). These results indicate that conjugation is a mechanism that could account for the resistance gene flux from gram-positive to gram-negative bacteria observed in nature.
authors.affiliation: Laboratoire d'Ecologie Microbienne, Institut Gustave-Roussy, Villejuif, France.
authors.forename: F
authors.initials: F
authors.lastname: Doucet-Populaire
authors.forename: P
authors.initials: P
authors.lastname: Trieu-Cuot
authors.forename: A
authors.initials: A
authors.lastname: Andremont
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
mesh_terms: D000818:Animals; D003227:Conjugation, Genetic / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics*; D004064:Digestive System / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D005856:Germ-Free Life; D051379:Mice; D008809:Mice, Inbred C3H; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial