Gas chromatographic/mass spectrometric identification of 3-hydroxydicarboxylic acids in urine.
Organic acid profiles were routinely analysed for two patients suffering from a massive 3-hydroxydicarboxyluria and five patients suffering from ketoaciduria. The identification of the urinary, saturated and unsaturated 3-hydroxydicarboxylic acids as their trimethylsilyl derivatives was performed with electron impact and positive chemical ionization gas chromatography/mass spectrometry. Additionally, their methylene units have been determined on SE-52 and OV-1701 types of stationary phases.
Verhaeghe
B J
BJ
Laboratoria voor Medische Biochemie en Klinische Analyse, Rijksuniversiteit Gent, Belgium.
Van Bocxlaer
J F
JF
De Leenheer
A P
AP
D003998:Dicarboxylic Acids / Q000652:urine*; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013058:Mass Spectrometry; D008661:Metabolism, Inborn Errors / Q000652:urine; D008928:Mitochondria / Q000378:metabolism; D008970:Molecular Weight
D003998:Dicarboxylic Acids; D005227:Fatty Acids