Lack of modifying effects of 6-mercaptopurine in a medium term bioassay system for liver carcinogenesis using male F344 rats.
Carcinogenic and modification potential of 6-mercaptopurine (6-MP) was studied in a medium-term bioassay system for rat liver carcinogenesis. F344 male rats were initiated with a single dose (200 mg/kg body wt.) of diethylnitrosamine (DEN) i.p. and fed diets containing either 0.005% or 0.02% 6-MP with or without 0.05% phenobarbital (PB) for 6 weeks. Quantitative data revealed that 6-MP did not enhance the appearance of enzyme-altered preneoplastic foci and nodules even when administered at the highest dose (0.02%) despite showing an immunosuppressive effect and slight liver cell damage. Neither of the doses of 6-MP exerted any significant influence on the enhancing effect of PB when administered simultaneously in the medium-term-bioassay.
Amanuma
T
T
Department of Oncological Pathology, Nara Medical College, Japan.
Maruyama
H
H
Tsujiuchi
T
T
Tsutsumi
M
M
Denda
A
A
Konishi
Y
Y
D000818:Animals; D001835:Body Weight; D004052:Diethylnitrosamine; D007958:Leukocyte Count; D008114:Liver Neoplasms, Experimental / Q000097:blood / Q000139:chemically induced*; D008297:Male; D015122:Mercaptopurine / Q000494:pharmacology*; D009929:Organ Size; D010634:Phenobarbital / Q000494:pharmacology; D011230:Precancerous Conditions / Q000097:blood / Q000139:chemically induced*; D051381:Rats; D011916:Rats, Inbred F344
D004052:Diethylnitrosamine; D015122:Mercaptopurine; D010634:Phenobarbital