Growth of Haemophilus influenzae type b in continuous culture: effect of dilution rate on outer-membrane protein and lipopolysaccharide expression.
Haemophilus influenzae type b (Hib) was grown in continuous culture under cystine-limitation between dilution rates (D) of 0.065-0.28 h-1. A similar outer-membrane protein profile, as adjudged by SDS-PAGE, was found at all dilution rates. However, a shift to a lipopolysaccharide structure with a greater electrophoretic mobility on SDS-PAGE with accompanying changes in monoclonal antibody reactivity was observed at D greater than or equal to 0.15 h-1. Growth rate per se can affect the expression of outer-membrane components of Hib.
Langford
P R
PR
Department of Paediatrics, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
Moxon
E R
ER
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000096:biosynthesis; D001431:Bacteriological Techniques; D003470:Culture Media; D003553:Cystine / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006193:Haemophilus influenzae / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000032:analysis* / Q000096:biosynthesis
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D008070:Lipopolysaccharides; D003553:Cystine