Isolation of Pseudomonas pickettii strains that degrade 2,4,6-trichlorophenol and their dechlorination of chlorophenols.
Three strains of Pseudomonas pickettii that can grow with 2,4,6-trichlorophenol (2,4,6-TCP) as the sole source of carbon and energy were isolated from different mixed cultures of soil bacterial populations that had been acclimatized to 2,4,6-TCP. These strains released 3 mol of chloride ion from 1 mol of 2,4,6-TCP during the complete degradation of the TCP. Of these strains, P. pickettii DTP0602 in high-cell-density suspension cultures dechlorinated various chlorophenols (CPs). Cells that were preincubated with 2,4,6-TCP converted isomers of 4-CP to the corresponding chloro-p-hydroquinones, but those preincubated with 4-CP converted CPs lacking a chlorine atom(s) at the o position to isomers of chlorocatechol. The ability of DTP0602 to dechlorinate 2,4,6-TCP was induced by 2,6-dichlorophenol, 2,3,6- and 2,4,6-TCP, and 2,3,4,6-tetrachlorophenol and was repressed in the presence of succinate or glucose.
Kiyohara
H
H
Biotechnology Laboratory, Department of Applied Chemistry, Okayama University of Science, Japan.
Hatta
T
T
Ogawa
Y
Y
Kakuda
T
T
Yokoyama
H
H
Takizawa
N
N
D001673:Biodegradation, Environmental; D002713:Chlorine / Q000378:metabolism; D002733:Chlorophenols / Q000378:metabolism* / Q000494:pharmacology; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology
D002733:Chlorophenols; D002713:Chlorine; C024564:2,4,6-trichlorophenol