Expression and translation of the growth hormone-receptor gene in the guinea-pig.
The refractoriness of guinea-pigs to the growth-promoting actions of exogenous GH has been suggested to be due to a deficiency or defect in tissue GH receptors or in GH-receptor gene expression. GH-receptor mRNA was, however, demonstrated by Northern blot analysis and by the polymerase chain reaction in extracts of guinea-pig liver, adipose tissue, brain, hypothalamus and pituitary gland. High-affinity, low-capacity binding sites for radio-labelled ovine GH were also demonstrated on the plasma membranes of guinea-pig liver and were similar to those in rat liver. These results demonstrate that the unresponsiveness of guinea-pigs to exogenous GH is not due to the absence of GH receptors.
Harvey
S
S
Department of Physiology, University of Alberta, Edmonton, Canada.
Fraser
R A
RA
D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry; D002462:Cell Membrane / Q000737:chemistry; D015870:Gene Expression / Q000235:genetics*; D006168:Guinea Pigs; D007031:Hypothalamus / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D010902:Pituitary Gland / Q000737:chemistry; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D011869:Radioligand Assay; D011986:Receptors, Somatotropin / Q000235:genetics*
D012333:RNA, Messenger; D011986:Receptors, Somatotropin