Surface-modified membranes as a matrix for protein purification.
Recently introduced membrane-based chromatographic supports for protein separation are available either with a coupled ligand, e.g., protein A, protein G or ion-exchange groups, or as activated matrices for coupling a desired ligand. The coupling conditions for protein A and immunoglobulin G to an epoxy-activated membrane were determined. The performance of the prepared affinity membranes was investigated using pure rabbit immunoglobulin G and protein A as a model system. For practical application monoclonal antibodies from cell culture supernatant were purified with a prepared protein A membrane and for comparison with a sulphonic acid ion exchange membrane.
Langlotz
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P
GBF-Gesellschaft für Biotechnologische Forschung mbH, Germany.
Kroner
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KH
D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000295:instrumentation*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D004591:Electrophoresis, Polyacrylamide Gel; D007074:Immunoglobulin G / Q000737:chemistry; D008567:Membranes, Artificial; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013205:Staphylococcal Protein A / Q000737:chemistry
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D008567:Membranes, Artificial; D011506:Proteins; D013205:Staphylococcal Protein A