title: Astrocytes derived from long-term primary cultures recapitulate features of astrogliosis as they re-enter the cell division cycle.
abstract: We investigated whether the initiation of cell cycling by astrocytes after prolonged quiescence in long-term primary cultures is associated with immunocytochemical changes that characterize reactive astrogliosis. Primary cultures of newborn rat brain were maintained for greater than 2 months in a stable quiescent state. Partially synchronous transition through a single cell cycle was achieved by trypsinization and replating, and then after 2-3 days, by 48 h of serum depletion and serum shift-up to 10% (time 0). At time 0, the percentages of cells decorated by monoclonal antibodies specific for bromodeoxyuridine (BrDU) after a 2.5 h pulse, and for glial fibrillary acidic protein (GFAP) and vimentin (VIM) were respectively 8 +/- 2, 15 +/- 4 and 10 +/- 3. By 24 h (S phase), 64 +/- 7% of nuclei were (BrDU+), and percentages of (GFAP+) and (VIM+) cells were 19 +/- 4 and 87 +/- 12, respectively. Dual label immunofluorescence showed that greater than 75% of (GFAP+) cells were indeed (VIM+/GFAP+) at 24 h, and that the percentages of (VIM+), (BrDU+) and (VIM+/BrDU+) cells were equivalent for the duration of the first cell cycle (36-48 h). By 72-96 h, (VIM+) cells decreased to less than 10%, and (BrDU+) cells numbered 32 +/- 8%, while (GFAP+) cells increased to around 90%. Ran-2 immunofluorescence at 96 h identified virtually all of the cells as type 1 astrocytes. Thus, astrocytes after months of quiescence give rise to cells that recapitulate a VIM/GFAP transition in a manner resembling astrogliosis, and do so in relation to progression through a single cell cycle.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Neurology, School of Medicine and Biomedical Sciences, State University of New York, Buffalo 14222.
authors.forename: T J
authors.initials: TJ
authors.lastname: Langan
authors.forename: M C
authors.initials: MC
authors.lastname: Slater
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001253:Astrocytes / Q000166:cytology*; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000502:physiology; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005911:Gliosis / Q000473:pathology*; D051381:Rats; D016196:S Phase / Q000502:physiology; D013997:Time Factors; D014746:Vimentin / Q000032:analysis
chemical_list: D000954:Antigens, Surface; D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin; D001973:Bromodeoxyuridine