title: Chitin-binding proteins in potato (Solanum tuberosum L.) tuber. Characterization, immunolocalization and effects of wounding.
abstract: Tubers of potato (Solanum tuberosum L.) contain a number of chitin-binding proteins which have possible functions in defence against pathogens. A major protein of the tuber is the chitin-binding lectin which has been further characterized with respect to its antigenicity and N-terminal amino acid sequence. By using an antiserum monospecific for tuber lectin in unwounded potato the protein was found in the cytoplasm and vacuole, unusually for a hydroxyproline-rich glycoprotein, but consistent with its soluble nature in subcellular extracts. Little increased synthesis of the lectin precursor or the post-translationally modified form could be demonstrated in excised potato tuber discs. However, after wounding there is increased synthesis of another hydroxyproline-containing glycoprotein of Mr 57,000, which binds to chitin and shares common epitopes with the lectin. In comparison with the tuber lectin, this novel glycoprotein contains less hydroxyproline, but from its overall composition it is clearly not an underhydroxylated form of the tuber lectin. It differed in its N-terminal amino acid sequence and was much less glycosylated, although arabinose was still present. Synthesis of the Mr-57,000 polypeptide began after the initial burst of protein synthesis and increased, reaching a peak at 24 h after wounding. The protein was produced with its enzymes of post-translational modification, prolyl hydroxylase and arabinosyltransferase, concomitantly with the marker enzymes for wounding, phenylalanine ammonia-lyase and membrane-bound phenol oxidase and peroxidase.
authors.affiliation: Department of Biological Sciences, City of London Polytechnic, U.K.
authors.forename: D J
authors.initials: DJ
authors.lastname: Millar
authors.forename: A K
authors.initials: AK
authors.lastname: Allen
authors.forename: C G
authors.initials: CG
authors.lastname: Smith
authors.forename: C
authors.initials: C
authors.lastname: Sidebottom
authors.forename: A R
authors.initials: AR
authors.lastname: Slabas
authors.forename: G P
authors.initials: GP
authors.lastname: Bolwell
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D015153:Blotting, Western; D002241:Carbohydrates / Q000032:analysis; D002686:Chitin / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006909:Hydroxyproline / Q000032:analysis; D007150:Immunohistochemistry; D007163:Immunosorbent Techniques; D037102:Lectins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D037121:Plant Lectins; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D011198:Solanum tuberosum / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D014617:Vacuoles / Q000737:chemistry
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D002686:Chitin; D006909:Hydroxyproline