title: Eight base changes are sufficient to convert a leucine-inserting tRNA into a serine-inserting tRNA.
abstract: Each aminoacyl-tRNA synthetase must functionally distinguish its cognate tRNAs from all others. We have determined the minimum number of changes required to transform a leucine amber suppressor tRNA to serine identity. Eight changes are required. These are located in the acceptor stem and in the D stem.
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena 91125.
authors.forename: J
authors.initials: J
authors.lastname: Normanly
authors.forename: T
authors.initials: T
authors.lastname: Ollick
authors.forename: J
authors.initials: J
authors.lastname: Abelson
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D005813:Genes, Synthetic; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D012356:RNA, Transfer, Leu / Q000235:genetics*; D012362:RNA, Transfer, Ser / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D009838:Oligodeoxyribonucleotides; D012356:RNA, Transfer, Leu; D012362:RNA, Transfer, Ser