Molecular cloning and sequence analysis of the mumps virus gene encoding the L protein and the trailer sequence.
We have cloned and determined the nucleotide sequences of the seventh gene of the Miyahara strain of mumps virus (MuV) encoding the L protein. The L gene is 6925 nucleotides in length and contains a single long open reading frame which is capable of coding for a protein of 2261 amino acids with a calculated molecular weight of 256,571 Da. The deduced amino acid sequence of the L protein of MuV showed significant homology with those of six other paramyxoviruses, human parainfluenza type 2 virus, Newcastle disease virus, Sendai virus, measles virus, human parainfluenza type 3 virus, and human respiratory syncytial virus. The predicted MuV L protein contained distinct elements thought to be essential for RNA polymerase activity. A noncoding sequence of 24 nucleotides downstream of the presumed polyadenylation site of the L gene showed significant complementarity with the leader sequence composed of 55 nucleotide at the 3' end of the genomic RNA.
Okazaki
K
K
Department of Measles Virus, National Institute of Health, Tokyo, Japan.
Tanabayashi
K
K
Takeuchi
K
K
Hishiyama
M
M
Okazaki
K
K
Yamada
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009109:Mumps virus / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D015183:Restriction Mapping; D015678:Viral Structural Proteins / Q000235:genetics*
D004279:DNA, Viral; D015678:Viral Structural Proteins; D004247:DNA; D012324:RNA-Dependent RNA Polymerase