Cloning, expression, and nucleotide sequence of a lipase gene from Pseudomonas fluorescens B52.
In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated from refrigerated raw milk. Sequence analysis revealed one major open reading frame of 1,428 nucleotides that was predicted to encode a protein with a molecular weight of 50,241. The predicted enzyme was found to contain an amino acid sequence highly homologous to the putative substrate-binding domain present within all lipases examined to date.
Tan
Y
Y
Department of Food Science, Pennsylvania State University, University Park 16802.
Miller
K J
KJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D005470:Fluorometry; D015870:Gene Expression; D005798:Genes, Bacterial; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping; D013379:Substrate Specificity
D004269:DNA, Bacterial; D008049:Lipase