The effect of storage in liquid nitrogen on the isolation of oral yeasts in human saliva.
Stimulated whole saliva samples were collected from a group of 127 6-yr-old schoolchildren. Each sample was divided into three parts. The first two, one of which contained added glycerol, were immediately frozen in liquid nitrogen and stored at -196 degrees C for 2 months. The third part was transferred to the laboratory and plated on selective medium for yeasts. Colony counts of the frozen and non-frozen samples were then compared. Statistical analysis showed a highly significant correlation between the counts for frozen and unfrozen samples. A high (40.1%) prevalence of yeast carriers was found, and Candida albicans was the most frequently recovered yeast.
Brambilla
E
E
Department of Dentistry and Stomatology, School of Medicine, University of Milan, Italy.
Strohmenger
L
L
Vogel
G
G
D002175:Candida / Q000145:classification / Q000302:isolation & purification*; D002176:Candida albicans / Q000302:isolation & purification; D002648:Child; D015925:Cryopreservation; D005615:Freezing; D005990:Glycerol / Q000008:administration & dosage; D006801:Humans; D009584:Nitrogen; D012044:Regression Analysis; D012463:Saliva / Q000382:microbiology*; D013048:Specimen Handling / Q000379:methods*
D009584:Nitrogen; D005990:Glycerol