Occurrence of wax esters and 1-O-alkyl-2,3-diacylglycerols in goat epididymal sperm plasma membrane.
Two unusual lipid classes were detected by thin-layer chromatography in the neutral lipids derived from goat cauda-epididymal sperm plasma membrane. The lipids were identified as wax esters and 1-O-alkyl-2,3-diacylglycerols based on chromatographic properties, identity of their hydrolysis products, and infrared/1H nuclear magnetic resonance spectral evidence. The membrane contained ca. 3 and 5 micrograms/mg protein of wax esters and alkyldiacylglycerols, respectively. The relative proportions of wax esters and alkyldiacylglycerols in the total neutral lipids were 1.5% and 2.4%, respectively. The lipids contained fatty acids with chain lengths of C14 to C22. The major fatty acids of the wax esters were 14:0, 16:0, 16:1 omega 7, 18:0 and 18:1 omega 9. The fatty acids in alkyldiacylglycerol were 16:0, 18:0, 22:5 omega 3 and 22:6 omega 3. Alkyldiacylglycerol was particularly rich in docosahexaenoic acid (22:6 omega 3) representing 30% of the total fatty acids. The alcohols of wax ester were all saturated with C20-C29 carbon chains. The deacylated products derived from alkyldiacylglycerols were identified as hexadecyl, octadecyl and octadec-9'-enyl glycerol ethers.
Rana
A P
AP
Indian Institute of Chemical Biology, Jadavpur, Calcutta.
Majumder
G C
GC
Misra
S
S
Ghosh
A
A
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry*; D004075:Diglycerides / Q000737:chemistry*; D004822:Epididymis / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry*; D006041:Goats; D008297:Male; D013094:Spermatozoa / Q000737:chemistry*; D014885:Waxes / Q000737:chemistry
D004075:Diglycerides; D005227:Fatty Acids; D014885:Waxes