Detection of proviral DNA and viral RNA in various tissues early after avian leukosis virus infection.
Using molecular biological techniques, a study was made of the tissue tropism of avian leukosis virus (ALV) early after infection. Two strains of chickens, one with and the other without endogenous viral genes, were infected with ALV of subgroup A immediately after hatching; specimens of nine tissues and blood samples were analyzed at various times thereafter. A polymerase chain reaction (PCR), specific to ALV subgroup A, was used to detect proviral DNA and viral RNA. In situ hybridization was used to confirm the presence of proviral DNA in tissue samples and to calibrate the PCR. The pattern of detection of proviral DNA and of ALV-RNA in the various tissues was similar for both chicken strains. At 2 weeks of age, ALV-RNA was demonstrated in all tissues tested: bursa of Fabricius, thymus, bone marrow, proventriculus, liver, spleen, kidney, muscle, gonads, and blood samples, and at 4 weeks of age all tissues contained proviral DNA. No tropism for a specific tissue was observed early after an ALV infection.
van Woensel
P A
PA
Central Veterinary Institute-DLO, Virology Department, Lelystad, The Netherlands.
van Blaaderen
A
A
Moorman
R J
RJ
de Boer
G F
GF
D000595:Amino Acid Sequence; D000818:Animals; D001353:Avian Leukosis / Q000235:genetics*; D002645:Chickens; D004279:DNA, Viral / Q000032:analysis*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D013997:Time Factors
D004279:DNA, Viral; D012367:RNA, Viral