Pancreas prostanoid production in ischemia and reperfusion.
This study was carried out to investigate the proportion of the 6-keto prostaglandin F1 alpha (6-keto PGF1 alpha) and thromboxane B2 (TXB2) alteration that is due to ischemia in pancreas transplantation against the proportion due to reperfusion. For this purpose, Lewis rats were divided in three experimental groups: Group I = Control, Group II = Donor pancreas subjected to 15 minutes of cold ischemia, Group III = Same as group II but pancreas were transplanted to the recipient individual and then subjected to reperfusion. The results indicate that increases in pancreas 6-keto PGF1 alpha occur as a consequence of cold ischemia while TXB2 remains unchanged. When blood flow was restored, 6-keto PGF1 alpha remained unchanged compared to the ischemic group while pancreatic levels of TXB2 were significantly increased. These results suggest a different induction of prostanoid metabolism during ischemia and reperfusion in pancreatic tissue.
Hotter
G
G
Department of Neurochemistry (Eicosanoid Branch), Centro de Investigacion y Desarrollo, CSIC, Barcelona, Spain.
Roselló-Catafau
J
J
Closa
D
D
Bulbena
O
O
López-Boado
M A
MA
Fernández-Cruz
L
L
Gelpi
E
E
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D007511:Ischemia / Q000378:metabolism; D008297:Male; D009926:Organ Preservation; D010179:Pancreas / Q000098:blood supply / Q000378:metabolism*; D016035:Pancreas Transplantation / Q000502:physiology*; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000378:metabolism*; D013929:Thromboxane B2 / Q000096:biosynthesis*
D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha