title: Evidence for tyrosine-linked glycosaminoglycan in a bacterial surface protein.
abstract: The S-layer protein of Acetogenium kivui was subjected to proteolysis with different proteases and several high molecular mass glycosaminoglycan peptides containing glucose, galactosamine and an unidentified sugar-related component were separated by molecular sieve chromatography and reversed-phase HPLC and subjected to N-terminal sequence analysis. By methylation analysis glucose was found to be uniformly 1,6-linked, whereas galactosamine was exclusively 1,4-linked. Hydrazinolysis and subsequent amino-acid analysis as well as two-dimensional NMR spectroscopy were used to demonstrate that in these peptides carbohydrate was covalently linked to tyrosine. As all of the four Tyr-glycosylation sites were found to be preceded by valine, a new recognition sequence for glycosylation is suggested.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried bei München.
authors.forename: J
authors.initials: J
authors.lastname: Peters
authors.forename: S
authors.initials: S
authors.lastname: Rudolf
authors.forename: H
authors.initials: H
authors.lastname: Oschkinat
authors.forename: R
authors.initials: R
authors.lastname: Mengele
authors.forename: M
authors.initials: M
authors.lastname: Sumper
authors.forename: J
authors.initials: J
authors.lastname: Kellermann
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: W
authors.initials: W
authors.lastname: Baumeister
mesh_terms: D000595:Amino Acid Sequence; D001421:Bacteria, Anaerobic / Q000737:chemistry*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D001426:Bacterial Proteins; D006025:Glycosaminoglycans / Q000737:chemistry*; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D006025:Glycosaminoglycans; D008562:Membrane Glycoproteins; D010455:Peptides; C055764:S-layer proteins; D014443:Tyrosine