title: Biodeuteration of poly(beta-hydroxybutyrate).
abstract: The formation of poly(beta-hydroxybutyrate), PHB, by Rhodobacter sphaeroides and Alcaligenes eutrophus was studied using the following carbon sources and solvents: (1), acetate in H2O; (2), D3-acetate in H2O; (3), acetate in 90 to 92% D2O; and (4), D3-acetate in 90 to 92% D2O. The growth of Rb. sphaeroides cultured under condition (2) showed no apparent deuterium isotope effect, while considerably slowed growth in the presence of D2O was observed under conditions (3) and (4). In all cases, the PHB produced under deuterium enriched conditions was of high molecular weight. Interestingly, comparatively high volumetric formation of partially deuterated PHB was obtained using culture condition (4) for A. eutrophus. Fourier transform infrared spectroscopy (FT-i.r.), pyrolysis gas chromatography mass spectrometry (PGC-m.s.), and nuclear magnetic resonance (n.m.r.) were used to establish the extent and distribution of deuterium in the PHB samples produced. Partially deuterated PHB was obtained in each case, using a deuterium enriched culture. Considerable differences in the extent and distribution of deuterium were found between micro-organisms and culture conditions.
authors.affiliation: Department of Polymer Science and Engineering, University of Massachusetts, Amherst 01003.
authors.forename: R A
authors.initials: RA
authors.lastname: Gross
authors.forename: H W
authors.initials: HW
authors.lastname: Ulmer
authors.forename: R W
authors.initials: RW
authors.lastname: Lenz
authors.forename: D J
authors.initials: DJ
authors.lastname: Tshudy
authors.forename: P C
authors.initials: PC
authors.lastname: Uden
authors.forename: H
authors.initials: H
authors.lastname: Brandt
authors.forename: R C
authors.initials: RC
authors.lastname: Fuller
mesh_terms: D000085:Acetates / Q000378:metabolism; D000421:Alcaligenes / Q000254:growth & development / Q000378:metabolism; D003903:Deuterium; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D012242:Rhodobacter sphaeroides / Q000254:growth & development / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D000085:Acetates; D003903:Deuterium; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates