Electrofractionation: a technique for detecting and recovering biomolecules.
Electrofractionation (EF) is a technique that allows electrophoretic materials to be detected and recovered following electrophoresis. The EF apparatus utilizes the resolving power of electrophoresis and the mobile phase of liquid chromatography to create a continuous elution system. Our data indicate that EF is able to detect and recover oligonucleotides, as DNA fragments, proteins, and presumably other material that can be analyzed by electrophoresis. EF shares functional similarities with high-performance electrophoresis chromatography (HPEC) but operates by a different strategy and at a fraction of the cost. Moreover, EF can be constructed largely from standard laboratory equipment. The simplicity, rapid analysis times, low cost, and high recovery yields of EF make this system a practical alternative to the conventional detection and purification methods used for biomolecules.
Shain
D H
DH
Department of Molecular Biology, University of Wyoming, Laramie 82071-3944.
Yoo
J
J
Slaughter
R G
RG
Hayes
S E
SE
Ji
T H
TH
D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002845:Chromatography / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D004560:Electricity; D004586:Electrophoresis / Q000379:methods; D009841:Oligonucleotides / Q000032:analysis* / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification
D009841:Oligonucleotides; D011506:Proteins; D004247:DNA