Surface components of HeLa cells that inhibit cytadherence of Chlamydia trachomatis.
Isolated HeLa plasma membrane (PM) preparations and extracts containing either cell-surface proteins or lipids were examined for inhibition of adherence of radiolabeled Chlamydia trachomatis serovar E elementary bodies to glutaraldehyde-fixed HeLa monolayers. A dose-dependent adherence-inhibitory activity could be demonstrated with the PM. A urea extract as well as lipids from HeLa cells also inhibited chlamydial cytadherence. The inhibitory activity of the PM was trypsin-sensitive. It was absent when the urea extract was prepared from trypsin-treated HeLa cells. The urea extract was subjected to electrophoresis and protein blotting using a native gel system. Probing with radiolabeled chlamydial cytadhesin showed a single protein present in the urea extract that could represent a HeLa cell protein receptor for the chlamydiae.
Joseph
T D
TD
Department of Microbiology, St. Louis University School of Medicine, Missouri 63104-1028.
Bose
S K
SK
D001422:Bacterial Adhesion; D002462:Cell Membrane / Q000502:physiology*; D002692:Chlamydia trachomatis / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008563:Membrane Lipids / Q000502:physiology; D008565:Membrane Proteins / Q000502:physiology
D008563:Membrane Lipids; D008565:Membrane Proteins