A two-dimensional YAC pooling strategy for library screening via STS and Alu-PCR methods.
We have developed a simple two-dimensional YAC pooling strategy to facilitate YAC library screening via STS and Alu-PCR approaches. The method has been implemented using the human total genomic YAC library of Olson and coworkers, and its validity tested by isolation of many chromosomes 19- and 21-specific YACs. The Alu-PCR approach is notable in that it is hybridization-based, such that PCR primer pairs do not need to be repeatedly synthesized and tested for each screening step.
Amemiya
C T
CT
Lawrence Livermore National Laboratory, Biomedical Sciences Division, Livermore, CA 94551.
Alegria-Hartman
M J
MJ
Aslanidis
C
C
Chen
C
C
Nikolic
J
J
Gingrich
J C
JC
de Jong
P J
PJ
D001483:Base Sequence; D015139:Blotting, Southern; D002272:Carcinoembryonic Antigen / Q000235:genetics; D015825:Chromosomes, Fungal; D015698:Genomic Library; D008969:Molecular Sequence Data; D009136:Muscular Dystrophies / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D016324:Sequence Tagged Sites
D002272:Carcinoembryonic Antigen; D009838:Oligodeoxyribonucleotides