Amino acid composition and immunochemical properties of AcPase III and AcPase IV representing glycoforms of the lower molecular weight, tartrate-resistant acid phosphatase of the frog liver.
1. Amino acid composition and immunological properties of the frog liver LMW AcPase forms: AcPase III and IV were examined. 2. AcPase III and IV show nearly identical amino acid composition and close immunological similarity. 3. These results indicate protein identity of both the enzyme forms and together with our previous data [Kubicz A., Szalewicz A. and Chrambach A., Int. J. Biochem. 23, 413-419 (1991)] demonstrate that generation of AcPase III and IV is a modification of the same enzyme protein by glycosylation processes. 4. Differences in immunoreactivity between AcPase III and IV were observed and discussed to be due to their altered conformations.
Szalewicz
A
A
Institute of Biochemistry, University of Wrocław, Poland.
Jańska
H
H
Strzelczyk
B
B
Kubicz
A
A
D000135:Acid Phosphatase / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D006031:Glycosylation; D005779:Immunodiffusion; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D011893:Rana esculenta; D013644:Tartrates / Q000494:pharmacology
D000596:Amino Acids; D013644:Tartrates; D000135:Acid Phosphatase; C029768:tartaric acid