Tiopronin (2-mercaptopropionylglycine) has chemokinetic and chemotactic properties for polymorphonuclear leukocytes.
Tiopronin enhances migration of polymorphonuclear leukocytes (PMNs). The enhancing effect consists partly of a chemokinetic, and partly of a chemotactic effect. Intact sulfhydryl groups on the outer surface of the PMN plasma membrane are required for the enhanced locomotion: pretreatment of cells with the non-penetrating sulfhydryl reagent (5,5'-dithiobis(2-nitrobenzoic acid) completely abolishes the activating effect of tiopronin, but has only a moderate effect on activation by fMet-Leu-Phe. Pretreatment of PMNs with pertussis toxin inhibits tiopronin-induced enhancement of migration, suggesting that a pertussis toxin-sensitive G-protein is involved in the enhancing effect of tiopronin on PMN migration.
Elferink
J G
JG
Department of Medical Biochemistry, University of Leiden, The Netherlands.
de Koster
B M
BM
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000502:physiology; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D011817:Rabbits; D008625:Tiopronin / Q000494:pharmacology*
D009240:N-Formylmethionine Leucyl-Phenylalanine; D004228:Dithionitrobenzoic Acid; D008625:Tiopronin; D019204:GTP-Binding Proteins