Full sequence of neurocalcin, a novel calcium-binding protein abundant in central nervous system.
We determined the cDNA sequence for neurocalcin, a novel calcium-binding protein in bovine brain. This clone (pCalN) has 582 nucleotides in the open reading frame including the termination codon TGA, 11 nucleotides of the 5' leader and 1251 nucleotides of the 3' noncoding region. The deduced amino acid sequence revealed that neurocalcin is composed of 193 amino acids, has a molecular mass of 22,284 daltons, and contains three putative calcium-binding sites (EF-hand motifs). By Northern blot analysis, 3.8kbp mRNA was detected in brain. The deduced amino acid sequence had a strong homology to visinin (46.5%) and recoverin (51.6%) in retina, suggesting that neurocalcin may play a visinin- or recoverin-like role in brain.
Okazaki
K
K
Department of Pharmacology, Nagoya University School of Medicine, Japan.
Watanabe
M
M
Ando
Y
Y
Hagiwara
M
M
Terasawa
M
M
Hidaka
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000235:genetics*; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002147:Calmodulin / Q000235:genetics; D002417:Cattle; D003001:Cloning, Molecular; D015342:DNA Probes; D004277:DNA, Single-Stranded / Q000235:genetics; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051596:Neurocalcin; D016133:Polymerase Chain Reaction; D044169:Receptors, Calcium-Sensing; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D015342:DNA Probes; D004277:DNA, Single-Stranded; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D044169:Receptors, Calcium-Sensing