6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae.
The addition of 6-azauracil to the growth medium causes a strong reduction of the GTP level in the nucleotide pool of Saccharomyces cerevisiae. In-vitro experiments show a strong inhibition of IMP dehydrogenase activity by 6-azaUMP explaining the preceding effect. PPR2 mutants, previously characterized by an increased sensitivity to 6-azauracil compared to the wild-type, are specifically susceptible to the lowering of the GTP pool, and are able to grow in presence of 6-azauracil when guanine is added to the medium.
Exinger
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IBMC du CNRS, UPR SMBMR 9002, Strasbourg, France.
Lacroute
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F
D000963:Antimetabolites / Q000494:pharmacology*; D006160:Guanosine Triphosphate / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D014498:Uracil / Q000031:analogs & derivatives* / Q000494:pharmacology
D000963:Antimetabolites; D014498:Uracil; D006160:Guanosine Triphosphate; C100248:azauracil