Interaction of large granular lymphocytes with susceptible target does not induce second messenger and cytolytic granule exocytosis.
We studied the activation signals of LGL, from LDGL patients, following incubation with susceptible K562 target cells. The findings showed that LGL lysis is independent of [Ca2+]i rise and cytolytic granule exocytosis, and most likely involves alternative, as yet unidentified, mechanisms.
Pollis
F
F
Institute of Oncology, Inter-University Center for Cancer Research, University of Padova, Italy.
Rosato
A
A
Bronte
V
V
Mandruzzato
S
S
Zambon
A
A
Zambello
R
R
Pizzo
P
P
Zanovello
P
P
D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D053804:Granzymes; D006801:Humans; D008232:Lymphoproliferative Disorders / Q000145:classification / Q000378:metabolism*; D015290:Second Messenger Systems; D012697:Serine Endopeptidases / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*
D053804:Granzymes; D012697:Serine Endopeptidases; C509212:GZMA protein, human; D002118:Calcium