title: Characterization of a phospholipase A2 in human serum.
abstract: Elevated catalytic activities of serum phospholipase A2 were measured in patients with inflammatory diseases. In contrast to human pancreatic phospholipase A2, the enzyme in serum of patients with non-pancreatic diseases was rather heat labile and showed a broad pH-optimum in the neutral range. Molecular sieving experiments revealed the existence of macro-molecular forms of the enzyme in serum which were cleaved into monomers of M(r) 14,000 in the presence of high salt concentrations. More than 100-fold purification was achieved by gel filtration chromatography on Sephadex G-100 in the presence of 2 mol/l KCl. Whether this serum phospholipase A2 and a secretory liver and platelet isoenzyme are identical remains to be established.
authors.affiliation: Institute of Clinical Chemistry, Bogenhausen Hospital, Munich, Federal Republic of Germany.
authors.forename: G E
authors.initials: GE
authors.lastname: Hoffmann
authors.forename: E
authors.initials: E
authors.lastname: Schneider
authors.forename: K
authors.initials: K
authors.lastname: Brand
authors.forename: V
authors.initials: V
authors.lastname: Kozumplik
authors.forename: S
authors.initials: S
authors.lastname: Fateh-Moghadam
mesh_terms: D001792:Blood Platelets / Q000201:enzymology; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000097:blood / Q000737:chemistry; D008970:Molecular Weight; D010741:Phospholipases A / Q000097:blood* / Q000737:chemistry; D054467:Phospholipases A2; D011487:Protein Conformation
chemical_list: D007527:Isoenzymes; D010741:Phospholipases A; D054467:Phospholipases A2