Inhibition of Meth-A tumor cell proliferation in combined use of disulfiram with catalase.
The effect of combined use of disulfiram with catalase on Meth-A tumor cell proliferation was studied. The simultaneous addition of 5 x 10(-7) or 1 x 10(-6) M disulfiram with catalase (4-40 micrograms/ml) induced marked inhibition of cell proliferation. A moderate degree of the antiproliferative effect was also obtained by pretreatment of the target cells with 5 x 10(-6) or 1 x 10(-5) M disulfiram in the presence of catalase (40 micrograms/ml). These results suggest that compounds or metabolites with cytostatic activity are newly formed following the reaction of disulfiram with catalase.
Mashiba
H
H
Division of Immunology, National Kyushu Cancer Center, Fukuoka, Japan.
Matsunaga
K
K
D000818:Animals; D002374:Catalase / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004221:Disulfiram / Q000494:pharmacology*; D015536:Down-Regulation; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D002374:Catalase; D004221:Disulfiram