Characterization of a 67 kDa microtubule-binding protein in the pancreas from different species.
Microtubule-interacting proteins have been studied from a pancreas supernatant. These proteins were first identified by affinity chromatography on taxol-stabilized microtubules. Among these interacting polypeptides, we show, for the first time, the presence of a protein which has a molecular mass of 67 kDa, as determined by polyacrylamide slab gel electrophoresis. The heat stability and the ability of this 67 kDa polypeptide to copolymerize with phosphocellulose-purified tubulin suggest that this protein may be a microtubule-associated protein.
Michalik
L
L
INSERM U61, Unité de Biologie Cellulaire et de Physiopathologie Digestives, Strasbourg, France.
Vanier
M T
MT
Launay
J F
JF
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D010179:Pancreas / Q000737:chemistry*; D011108:Polymers; D051381:Rats; D013552:Swine; D014404:Tubulin / Q000737:chemistry*
D008869:Microtubule-Associated Proteins; D011108:Polymers; D014404:Tubulin