Heterogeneity and regulation of manganese peroxidases from Phanerochaete chrysosporium.
Lignin and Mn peroxidases are two families of isozymes produced by the lignin-degrading fungus Phanerochaete chrysosporium under nutrient nitrogen or carbon limitation. We purified to homogeneity the three major Mn peroxidase isozymes, H3 (pI = 4.9), H4 (pI = 4.5), and H5 (pI = 4.2). Amino-terminal sequencing of these isozymes demonstrates that they are encoded by different genes. We also analyzed the regulation of these isozymes in carbon- and nitrogen-limited cultures and found not only that the lignin and Mn peroxidases are differentially regulated but also that differential regulation occurs within the Mn peroxidase isozyme family. The isozyme profile and the time at which each isozyme appears in secondary metabolism differ in both nitrogen- and carbon-limited cultures. Each isozyme also responded differently to the addition of a putative inducer, divalent Mn. The stability of the Mn peroxidases in carbon- and nitrogen-limited cultures was also characterized after cycloheximide addition. The Mn peroxidases are more stable in carbon-limited cultures than in nitrogen-limited cultures. They are also more stable than the lignin peroxidases. These data collectively suggest that the Mn peroxidase isozymes serve different functions in lignin biodegradation.
Pease
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Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Tien
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D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D003470:Culture Media / Q000494:pharmacology; D004795:Enzyme Stability; D005658:Fungi / Q000201:enzymology*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D003470:Culture Media; D007527:Isoenzymes; D012333:RNA, Messenger; D008345:Manganese; D010544:Peroxidases; C051129:manganese peroxidase