Evaluation of total protein content in tears of dogs by polyacrylamide gel disk electrophoresis.
Concentration of total proteins was measured and sodium dodecyl sulfate-polyacrylamide gel disk electrophoresis was performed on tear and plasma samples obtained from 26 healthy dogs, and the results were compared. Mean +/- SEM concentration of total proteins in tears was 0.63 +/- 0.04 g/dl, and significant effects of age or gender were not found. The protein composition of tears in dogs was complex, and bands from light and heavy chains of immunoglobulins were identified by electrophoresis.
Barrera
R
R
Departamento de Medicina y Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
Jiménez
A
A
López
R
R
Mañé
M C
MC
Rodríguez
J F
JF
Molleda
J M
JM
D000367:Age Factors; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D003720:Densitometry; D004285:Dogs / Q000378:metabolism*; D004589:Electrophoresis, Disc; D005136:Eye Proteins / Q000032:analysis*; D005260:Female; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D007147:Immunoglobulin Light Chains / Q000032:analysis; D008297:Male; D012016:Reference Values; D013666:Tears / Q000737:chemistry*
D001798:Blood Proteins; D005136:Eye Proteins; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; C042434:tear proteins