title: Isozyme variability and differentiation between Rhodnius prolixus, R.robustus and R.pictipes, vectors of Chagas disease in Venezuela.
abstract: Enzyme polymorphism in triatomine bugs of the genus Rhodnius (Hemiptera: Reduviidae), vectors of Chagas disease, is analysed using both starch and polyacrylamide gel electrophoresis. Out of forty-five enzymes assayed, the electromorphs of seventeen of them: AO, CA, DIA, ES, ES-A, FH, GPD, G6PD, GPI, MDH, ME, 6PGD, PGM, ACON, ACPH, LAP and SOD, involving twenty-two putative structural loci, were scorable. These gene-enzyme systems were therefore selected for routine characterization of R.prolixus Stal adults from different strains. The first thirteen enzymes, involving sixteen structural loci, were also analysed in first instar nymphs of the three species, R.prolixus, R.robustus Larrousse and R.pictipes Stal. Allelic frequencies were calculated for three R.prolixus strains: three to five loci appeared to be polymorphic. The proportion of polymorphic loci (22%) and the average heterozygosity (0.06) indicated low genetic variability, with significant differences between the strains at individual loci. Rhodnius prolixus and R.robustus were found to have identical isozymic patterns. R.pictipes was genetically well differentiated, with twelve diagnostic loci.
authors.affiliation: Unité d'Ecopathologie, INRA, La Minière, Guyancourt, France.
authors.forename: M
authors.initials: M
authors.lastname: Harry
authors.forename: I
authors.initials: I
authors.lastname: Galindez
authors.forename: M L
authors.initials: ML
authors.lastname: Cariou
mesh_terms: D000483:Alleles; D000818:Animals; D014355:Chagas Disease / Q000635:transmission*; D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007303:Insect Vectors / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D008297:Male; D009758:Nymph / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D010641:Phenotype; D012239:Rhodnius / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D014687:Venezuela
chemical_list: D007527:Isoenzymes