Compartmental study of rat renal phospholipid metabolism.
Phospholipid content and metabolism were studied in rat renal papillary, medullary and cortical slices. The highest concentration of phospholipids was found in cortex and the lowest in papilla samples (ratio cortex/medulla, 1.3; cortex/papilla, 3.7). The profile of the various phospholipids was different depending on the zone. The most important difference was the relative concentrations of sphingomyelin (CerPCho) and phosphatidylinositol (PtdIns) with ratios for PtdIns/CerPCho of 5.0, 3.3 and 2.5 in papilla, medulla, and cortex, respectively. In the three zones, PtdIns showed the highest specific activity for [2-14C]glycerol and [1-14C]arachidonic acid incorporation. By contrast, a higher amount of [1-14C]palmitic acid was incorporated into phosphatidylcholine than into any other phospholipid. The various radioactive precursors were only poorly incorporated into phosphatidylethanolamine. No radioactivity was associated with phosphatidylserine. The papilla possesses the most active phospholipid metabolism of all the pathways studied.
Sterin-Speziale
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Departamento de Química Biológica, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina.
Kahane
V L
VL
Setton
C P
CP
Fernandez
M C
MC
Speziale
E H
EH
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D066298:In Vitro Techniques; D007668:Kidney / Q000737:chemistry / Q000378:metabolism*; D007672:Kidney Cortex / Q000737:chemistry / Q000378:metabolism; D007679:Kidney Medulla / Q000737:chemistry / Q000378:metabolism; D007682:Kidney Pelvis / Q000737:chemistry / Q000378:metabolism; D008297:Male; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D005227:Fatty Acids; D010169:Palmitic Acids; D010743:Phospholipids; D016718:Arachidonic Acid; D019308:Palmitic Acid