Reproducible imaging and dissection of plasmid DNA under liquid with the atomic force microscope.
Reproducible images of uncoated DNA in the atomic force microscope (AFM) have been obtained by imaging plasmid DNA on mica in n-propanol. Specially sharpened AFM tips give images with reproducible features several nanometers in size along the DNA. Plasmids can be dissected in propanol by increasing the force applied by the AFM tip at selected locations.
Hansma
H G
HG
Department of Physics, University of California, Santa Barbara 93106.
Vesenka
J
J
Siegerist
C
C
Kelderman
G
G
Morrett
H
H
Sinsheimer
R L
RL
Elings
V
V
Bustamante
C
C
Hansma
P K
PK
D004247:DNA / Q000648:ultrastructure*; D004279:DNA, Viral / Q000648:ultrastructure; D004577:Electron Probe Microanalysis / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010957:Plasmids; D011433:Propranolol
D004279:DNA, Viral; D004247:DNA; D011433:Propranolol