Enzyme immunoassay of angiotensin II in human plasma.
We established a highly sensitive double-antibody enzyme immunoassay (EIA) for angiotensin (ANG) II. For competitive reactions, the ANG II-antibody was incubated with ANG II standard (or sample) and beta-D-galactosidase-labeled ANG III (delayed addition). Free and antibody-bound labeled antigen were separated using an anti-rabbit immunoglobulin G (IgG) coated immunoplate. The enzyme activity on the plate was fluorometrically determined. The present immunoassay allows detection of 0.4 to 72 fmol/well of ANG II. Using the present EIA, ANG II-like immunoreactivity (-LI) in human plasma was determined. The level of ANG II-LI in human plasma from 10 healthy volunteers was 33.3 +/- 10.4 pmol/l.
Yasunaga
F
F
Department of Hospital Pharmacy, Medical College of Oita, Japan.
Kawano
A
A
Morikawa
N
N
Takeyama
M
M
D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data
D007106:Immune Sera; D000804:Angiotensin II