Serum A1 and B apolipoprotein determination: comparison of an immunoturbidimetric method with a monoclonal-antibody-based radial immunodiffusion assay.
Epidemiological and clinical evidence have indicated that apolipoprotein A1 and B determination can better define the lipoprotein pattern in normal subjects and in subjects with coronary heart disease. In this paper, a recent immunoturbidimetric method for routine apolipoprotein A1 and B measurement (using the Turbitimer system and commercially available antisera) has been evaluated. The precision and the accuracy of the method have been previously considered. Within-run and between-run coefficients of variation (ranging from 1.67% to 5.04%) for both assays indicate good precision of the method. Accuracy was evaluated on 2 consecutive days (n = 10 each run) using a standard serum for apolipoprotein A1 and B. The bias obtained was 3.79% for apolipoprotein A1 and 2.30% for B. Apolipoproteins A1 and B were then measured in 100 normal and hyperlipemic sera with the immunoturbidimetric assay and radial immunodiffusion (using the monoclonal antibodies). The data obtained were evaluated by linear regression analysis (Al, r = 0.893; B, r = 0.862). The good correlation between the two methods suggests that the immunoturbidimetric assay can be usefully performed for routine apolipoprotein A1 and B determination because of its lower cost, rapidity, and simplicity.
Postiglione
L
L
Department of Internal Medicine, 2nd Medical School, University of Naples, Italy.
Spanò
A
A
Varricchio
P
P
Larizza
G
G
Oriente
A
A
Gattozzi
D
D
Oriente
P
P
D000911:Antibodies, Monoclonal / Q000276:immunology*; D016632:Apolipoprotein A-I / Q000032:analysis*; D001055:Apolipoproteins B / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D005779:Immunodiffusion; D009391:Nephelometry and Turbidimetry; D011233:Precipitin Tests; D011237:Predictive Value of Tests
D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I; D001055:Apolipoproteins B