The isolation and partial characterization of trypsinogen, pancreatic secretory trypsin inhibitor and multiple forms of chymotrypsinogen and trypsin from the pancreas of the ostrich (Struthio camelus).
1. PSTI, two chymotrypsinogens and two trypsins were purified to homogeneity by acid extraction, salt fractionation, SP-Sephadex C-50 chromatography and RP-HPLC. 2. A third chymotrypsinogen, a trypsinogen and another trypsin were purified using an alkaline extraction procedure, followed by Trasylol- and Benzamidine-Sepharose affinity chromatography and hydroxylapatite chromatography. 3. The enzymes differed in amino acid composition as well as in specific activities towards synthetic amidase and esterase substrates. 4. N-terminal amino acid sequences were determined for one chymotrypsinogen and one trypsin.
Smith
N
N
Department of Biochemistry, University of Port Elizabeth, South Africa.
Naudé
R J
RJ
Oelofsen
W
W
Lazure
C
C
Patthy
A
A
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001717:Birds; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002919:Chymotrypsinogen / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid; D014357:Trypsin / Q000302:isolation & purification* / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D014362:Trypsinogen / Q000302:isolation & purification* / Q000378:metabolism
D000596:Amino Acids; D014361:Trypsin Inhibitors; D014362:Trypsinogen; D002919:Chymotrypsinogen; D014357:Trypsin