Hepatic concentration of vitamin K active compounds after application of phylloquinone to chickens on a vitamin K deficient or adequate diet.
Liver and serum concentrations of vitamin K active compounds were measured in two groups of (deficient and normal) broilers after administration of phylloquinone 1 mg/kg. Assays were performed by HPLC after extraction and purification of these compounds. The only menaquinone found in the chicken was menaquinone-4. In the deficient group, the chickens exhibited hepatic concentrations of vitamin K1, vitamin K1 epoxide and menaquinone-4 markedly lower than those of the control group. After administration of phylloquinone, vitamin K and vitamin K epoxide levels fell sharply. There is no hepatic storage of vitamin K comparable to that of vitamin A. However, while menaquinone levels were found to be stable in the control group, they rose significantly in the deficient group after vitamin K injection. The question is: is there a transformation of vitamin K into menaquinone and/or is there a preferential utilization of one of the vitamin K active compounds?
Guillaumont
M
M
Laboratory of Clinical Pharmacology, Hôpital Debrousse, Lyon, France.
Weiser
H
H
Sann
L
L
Vignal
B
B
Leclercq
M
M
Frederich
A
A
D000818:Animals; D002645:Chickens; D004032:Diet; D005260:Female; D007275:Injections, Intravenous; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D014812:Vitamin K / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood / Q000378:metabolism; D010837:Vitamin K 1 / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood; D024482:Vitamin K 2 / Q000031:analogs & derivatives; D014813:Vitamin K Deficiency / Q000097:blood / Q000378:metabolism*
D024482:Vitamin K 2; D014812:Vitamin K; C016186:vitamin K1 oxide; C030814:menatetrenone; D010837:Vitamin K 1