Anion permeation limits the uncoupling activity of fatty acids in mitochondria.
The action of such membrane-permeant cations as tetraphenyl phosphonium and dibenzyldimethyl ammonium upon fatty acid-uncoupled respiration has been studied with oligomycin-inhibited rat liver mitochondria. Both cations enhance fatty acid-stimulated respiration. This synergistic effect is explained by a facilitated permeation of the fatty acid anion across the inner membrane due to an ion-pair complex. It is concluded that fatty acid uncoupling in rat liver mitochondria is limited by fatty acid anion permeation.
Schönfeld
P
P
Institut für Biochemie, Medizinischen Akademie Magdeburg, Germany.
D019297:2,4-Dinitrophenol; D000818:Animals; D000838:Anions / Q000378:metabolism*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002463:Cell Membrane Permeability; D004140:Dinitrophenols / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000378:metabolism*; D009861:Onium Compounds / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D014475:Uncoupling Agents / Q000378:metabolism*
D000838:Anions; D004140:Dinitrophenols; D005227:Fatty Acids; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D014475:Uncoupling Agents; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D019297:2,4-Dinitrophenol; D010100:Oxygen; C013289:tetraphenylphosphonium