3,5,3'-Triiodothyronine binding sites in synaptosomes from brain of chick embryo. Properties and ontogeny.
In this study we have demonstrated the presence of specific 3,5,3'-L-triiodothyronine (T3) binding sites in the synaptosomes of chick embryo cerebral cortex and described their ontogeny. Scatchard analyses of binding data obtained with synaptosomal preparations from 17-day-old embryos revealed two T3 binding sites. The first site (N1) had a high affinity and low capacity since its dissociation constant (Kd) was 68 +/- 1.3 nM T3 (mean +/- S.D.; n = 3-5) and its maximal binding capacity (Bmax) was 8.63 +/- 1.59 ng T3/mg of protein, whereas the second site (N2) had a higher Kd of 5.04 +/- 0.5 microM T3 and a larger Bmax of 405 +/- 49 ng T3/mg of protein. The relative affinity of the synaptosomal fraction for T3 and other analogs was the following: T3 greater than T4 (thyroxine) greater than D-T3 (3,5,3'-D-triiodothyronine) = TRIAC (triiodothyroacetic acid) greater than rT3 (reverse T3). Gel chromatography of the [125I]T3 labeled fraction revealed a partially saturable peak with an estimated MW of more than 100 kDa. The ontogenic pattern showed a progressive increase of Kd and Bmax of N1, occurring mainly between the 12 and 19 days of incubation, and a marked fall, particularly of the Bmax, after hatching. The second site did not show any important variation during the embryogenesis. These data indicate the existence of specific T3 binding sites in synaptosomes from cerebral cortex of chick embryo, whose properties and ontogeny are completely different from those of the nuclear receptor.(ABSTRACT TRUNCATED AT 250 WORDS)
Giguère
A
A
Département de Médecine, Faculté de Médecine, Université de Sherbrooke, Qué., Canada.
Lehoux
J G
JG
Gallo-Payet
N
N
Bellabarba
D
D
D000818:Animals; D001667:Binding, Competitive / Q000502:physiology; D002540:Cerebral Cortex / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development; D002642:Chick Embryo; D002645:Chickens; D002850:Chromatography, Gel; D008854:Microscopy, Electron; D011988:Receptors, Thyroid Hormone / Q000032:analysis*; D013574:Synaptosomes / Q000737:chemistry* / Q000648:ultrastructure
D011988:Receptors, Thyroid Hormone