Construction of a plasmid containing the complete coding region of human elongation factor 2.
A plasmid pUChEF-2 containing the coding sequence as well as the complete 3'-untranslated region (3'UTR) of human EF-2 mRNA was constructed. The plasmid construct was assembled from a cDNA insert of pHGR81 (Rapp et al., (1988) Biol. Chem. Hoppe-Seyler 369, 247-250) comprising the C-terminal portion of the coding region and the 3'UTR, as well as a polymer chain reaction PCR fragment (Rapp et al., (1989) Biol. Chem. Hoppe-Seyler 370, 1071-1075) covering the missing part of the coding region from the amino-terminus.
Hanes
J
J
Laboratory of Genetic Engineering, Slovak Academy of Sciences, Bratislava, CSFR.
Freudenstein
J
J
Rapp
G
G
Scheit
K H
KH
D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D005260:Female; D005818:Genetic Engineering; D005822:Genetic Vectors; D006107:Granulosa Cells; D006801:Humans; D008969:Molecular Sequence Data; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000737:chemistry
D003062:Codon; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D012333:RNA, Messenger