title: Identification of an interleukin-6 responsive element and characterization of the proximal promoter region of the rat hemopexin gene.
abstract: The rat hemopexin (Hx) gene was isolated and studies of its transcriptional regulation initiated. For analysis by a transient expression assay, the sequence between -2400 and +21 and sequential 5' truncates were linked to the chloramphenicol acetyltransferase (CAT) gene. HepG2 cells transfected with these CAT constructs were treated with conditioned medium of lipopolysaccharide stimulated human monocytes, interleukin-1 (IL-1) or interleukin-6 (IL-6). The activities of putative regulatory regions joined to the SV40 promoter indicated that the flanking region of the rat Hx gene from -209 to -104 contains three functional regions designated proximal regulatory regions; PRR-I (-209 to -173), -II (-178 to -158) and -III (-154 to -104). We found that PRR-II contains a different class of IL-6 responsive element (RE) from that reported for the human Hx gene, and that PRR-I and PRR-III participate in the basal expression of rat Hx in HepG2 cells.
authors.affiliation: Department of Pediatrics, Cornell University Medical College, New York, New York 10021.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagae
authors.forename: U
authors.initials: U
authors.lastname: Muller-Eberhard
mesh_terms: D000210:Acute-Phase Reaction / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D005786:Gene Expression Regulation; D006466:Hemopexin / Q000235:genetics*; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D006466:Hemopexin