Purification and characterization of PSP-I and PSP-II, two major proteins from porcine seminal plasma.
Two major glycoproteins, designated PSP-I and PSP-II, were purified from porcine seminal plasma by ammonium sulfate fractionation, CM-cellulose chromatography, gel filtration on Sephadex G-75 (superfine), and reverse phase high performance liquid chromatography. These two proteins exist in several forms differing mainly in the carbohydrate moiety. The complete amino acid sequence of PSP-I has been determined by automated Edman degradation of peptides generated by proteolytic digestion and cyanogen bromide cleavage. The protein is 109 residues long and has a single glycosylation site at the asparagine residue at position 47. In addition, the N-terminal sequence of PSP-II has also been determined. PSP-I is a unique protein; a sequence homology search using the protein data base did not reveal any significant homology with other proteins. PSP-II shares 50% sequence homology with a family of zona pellucida-binding glycoproteins at the N-terminus.
Rutherfurd
K J
KJ
Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California 91010-0269.
Swiderek
K M
KM
Green
C B
CB
Chen
S
S
Shively
J E
JE
Kwok
S C
SC
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D006868:Hydrolysis; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012661:Semen / Q000378:metabolism*; D029589:Seminal Plasma Proteins; D029607:Seminal Vesicle Secretory Proteins; D013552:Swine
D000596:Amino Acids; D006023:Glycoproteins; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; D029607:Seminal Vesicle Secretory Proteins; C043076:beta-microseminoprotein; C074521:seminal vesicle secretory protein II, porcine; C074520:seminal vesicle secretory protein 109, porcine