Banding profiles of LTR of human endogenous retrovirus HERV-A in 24 chromosomes in somatic cell hybrids.
The human genome carries multiple copies of sequences related to endogenous retroviral genomes. We investigated the distribution of one of these sequences, HERV-A, in 24 human chromosomes by Southern analyses using DNAs from flow-sorted chromosomes or rodent cells carrying a single human chromosome. The results showed that HERV-A is distributed among all human chromosomes and that each chromosome has a specific Southern blot profile. The chromosome-specific pattern did not show significant polymorphism, except in a few cases, when the same chromosome obtained from different individuals was compared. These chromosome-specific Southern hybridization profiles may be useful for chromosome karyotyping. This would allow the integrity of human chromosomes in human-rodent somatic cell hybrids to be monitored without using conventional cytogenetic methods.
Sugino
H
H
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Oshimura
M
M
Matsubara
K
K
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D006224:Cricetinae; D003412:Cricetulus; D016172:DNA Fingerprinting; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D051379:Mice; D011110:Polymorphism, Genetic; D011533:Proviruses / Q000302:isolation & purification*; D044469:Racial Groups; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*; D016662:Virus Integration
D004279:DNA, Viral