Ion selectivity of Ba2+ inward current oscillations in ras-transformed fibroblasts that elicit cytoplasmic Ca2+ oscillations by bradykinin.
Ion selectivity of divalent cations on Ba2+ inward current oscillations was examined by voltage-clamp recording in v-Ki-ras-transformed NIH/3T3 (DT) fibroblasts where repetitive transient increases in cytoplasmic Ca2+ concentration were evoked by bradykinin. Application of bradykinin onto DT cells in 50 mM Ba2+ solution initiated Ba2+ inward current oscillations. The inward currents were inhibited in equimolar Sr2+ or Ca2+ solutions. Ba2+ current oscillations were dependent upon extracellular Ba2+ concentration. The results suggest that inward current oscillations are highly selective to Ba2+.
Higashida
H
H
Department of Biophysics, Kanazawa University School of Medicine, Japan.
Shahidullah
M
M
Hoshi
N
N
Noda
M
M
Hashii
M
M
Zhong
Z G
ZG
Nozawa
Y
Y
D016475:3T3 Cells; D000818:Animals; D001464:Barium / Q000378:metabolism; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism*; D002472:Cell Transformation, Viral / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects*; D011905:Genes, ras / Q000235:genetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008839:Microelectrodes; D013324:Strontium / Q000378:metabolism
D002413:Cations, Divalent; D001464:Barium; D001920:Bradykinin; D002118:Calcium; D013324:Strontium