[Effect of depletion of glucocorticoid receptor in the production of multiple organ failure during endotoxemia].
Endotoxin (20 mg/kg B.W.) was injected intravenously to control (n = 12) and glucocorticoid receptor (GR) depletion rat model (n = 13, in which more than 80 percent of GR were blocked with a potent GR antagonist RU486). Four hours after injection of endotoxin, the changes in serum acid phosphatase (ACP) activity, phospholipase A2 (PLA2) activity and lipid peroxide (LPO) level were determined. The results showed that all of the indices were increased in the control rats after endotoxin injection (ACP 17.6 +/- 1.9 U/dL, PLA2 325.6 +/- 99.1 U, and LPO 6.0 +/- 0.4 nmol MDA.ml-1), but they were increased to even higher levels in the rats injected with both RU486 and endotoxin (ACP 30.1 +/- 7.6 U/dL, PLA2 633.7 +/- 70.6 U, and LPO 8.2 +/- 1.1 nmol MDP.ml-1). Furthermore, after presented multiple organ damage pathologically. These results suggested that the glucocorticoid insufficiency caused by the decrease of GR after severe trauma or shock may exacerbate the damage of endotoxin to the organs, so much so that glucocorticoid insufficiency might result in multiple organ failure. This article also discussed problem of glucocorticoid treatment.
Fan
J
J
Department of Pathophysiology, Second Military Medical University.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D004731:Endotoxins; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D009102:Multiple Organ Failure / Q000201:enzymology / Q000209:etiology / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D014115:Toxemia / Q000150:complications*
D004731:Endotoxins; D008054:Lipid Peroxides; D011965:Receptors, Glucocorticoid; D010741:Phospholipases A; D054467:Phospholipases A2; D000135:Acid Phosphatase