The genetic organization of the mau gene cluster of the facultative autotroph Paracoccus denitrificans.
The mau gene cluster from Paracoccus denitrificans was cloned. The regions of a cloned fragment carrying genes for the small and the large subunit of the methylamine dehydrogenase were identified and sequenced. Open reading frames for the MADH small subunit gene and the MADH large subunit gene were identified. Three other open reading frames coding polypeptides with unknown function were found in the sequence. The small subunit gene sequence data reveal that the MADH small subunit polypeptide from P. denitrificans has an unusual leader sequence and contains the tryptophan tryptophyl quinone cofactor. The MADH small subunit genes and the parts of the open reading frames found upstream of them in the genome of M. extorquens AM1 and P. denitrificans have considerable similarity. The sequence data have been used for refinement of the X-ray crystallographic structure of the MADH from P. denitrificans, and key conserved residues have been identified.
Chistoserdov
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W.M. Keck Laboratories 138-78, California Institute of Technology, Pasadena 91125.
Boyd
J
J
Mathews
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FS
Lidstrom
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ME
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics*; D010231:Paracoccus denitrificans / Q000201:enzymology / Q000235:genetics*; D011487:Protein Conformation; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014961:X-Ray Diffraction
D004269:DNA, Bacterial; D046911:Macromolecular Substances; C021567:methylamine dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors