A new microtitration method for the enumeration of contagious bovine pleuropneumonia (CBPP) vaccines.
A new method is proposed for the microtitration of CBPP vaccines by performing eight parallel endpoint two-fold dilutions. When performed on a 96-well plate, it gives a titre with a precision of +/- 0.2 log10. By comparison, the established method, performed in tubes, gives a precision of only +/- 0.6 log10. The adaptation to microtitre plates allows much more economical culture of larger numbers of samples. Statistical analysis permits determination of vial-to-vial homogeneity of a batch and calculation of a titre with a precision of +/- 0.09 log10. The new method may be applied for the titration of mycoplasmas.
Thiaucourt
F
F
French Veterinary Mission in Ethiopia, Maisons Alfort.
Dí Maria
A
A
D001428:Bacterial Vaccines / Q000032:analysis* / Q000592:standards; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D015169:Colony Count, Microbial / Q000379:methods / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D009176:Mycoplasma mycoides / Q000276:immunology* / Q000302:isolation & purification; D011786:Quality Control
D001428:Bacterial Vaccines