DNA ploidy, S-phase fraction and cytomorphometry in relation to survival of human penile cancer.
The prognostic significance of DNA ploidy, DNA index, S-phase fraction (SPF) and median nuclear size was studied in 11 patients with squamous cell carcinoma of the penis. These patients have been followed for a minimum of 7 months after diagnosis. Nuclear DNA content was determined by flow cytometry from paraffin-embedded tissue. Patients with DNA diploid cancer (n = 7, 64%) had a better survival rate than patients with aneuploid cancer, and a small SPF was associated with a favorable outcome. A statistically significant relation between median nuclear size and survival could be demonstrated. Small modal nuclear size associated with poorer prognosis. There was a significant difference in survival between metastatic and nonmetastatic groups of tumors during the follow-up period. This study suggests that flow cytometric determination of nuclear DNA ploidy from paraffin-embedded samples in penile cancer does add an additional prognostic determinant in addition to the clinical staging of tumors.
Yu
D S
DS
Department of Surgery, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Chang
S Y
SY
Ma
C P
CP
D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000401:mortality* / Q000473:pathology; D002467:Cell Nucleus / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000235:genetics*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010412:Penile Neoplasms / Q000235:genetics / Q000401:mortality* / Q000473:pathology; D010413:Penis / Q000648:ultrastructure; D011003:Ploidies; D011379:Prognosis; D016196:S Phase; D016019:Survival Analysis
D004273:DNA, Neoplasm