title: Antibiotic sensitivities of Neisseria meningitidis isolates from patients and carriers in Greece.
abstract: Usage of antibiotics in southern Europe is less well regulated than in northern countries. The proportion (48%) of meningococci in Spain insensitive to pencillin (MIC greater than or equal to 0.1 mg/l) prompted this investigation of antibiotic sensitivities of isolates from Greek patients with meningitis (31) and carriers (47 school-children and 472 recruits). The agar dilution method was used to determine MIC to penicillin G (PN), sulphamethoxazole (SU), rifampicin (RF), cefaclor (CF) and ciprofloxacin (CP). The proportion of isolates insensitive to PN was 48% for isolates from patients, 19% from school-children and 36.6% from recruits. Resistance to SU (MIC greater than or equal to 16 mg/l) was found in 16% of those from patients, 10.6% from children and 40% from recruits. None of the isolates from patients was resistant to RF (greater than or equal to 1 mg/l) but 6% of those from carriers were. Resistance to CF (greater than or equal to 4 mg/l) was found in 9.2% of patient isolates, 6.4% from children and 23.7% from recruits. All isolates except one were sensitive to CP (MIC range less than 0.0015-0.125 mg/l). Resistances to PN, SU and RF were analysed by serogroup, serotype and subtype of the bacteria. The proportion of resistant isolates showed some variation between different areas of Greece, but it was not statistically significant.
authors.affiliation: National Meningitis Reference Laboratory, Hellenic Institute Pasteur, Athens, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Tzanakaki
authors.forename: C C
authors.initials: CC
authors.lastname: Blackwell
authors.forename: J
authors.initials: J
authors.lastname: Kremastinou
authors.forename: C
authors.initials: C
authors.lastname: Kallergi
authors.forename: G
authors.initials: G
authors.lastname: Kouppari
authors.forename: D M
authors.initials: DM
authors.lastname: Weir
mesh_terms: D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis; D002353:Carrier State / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D004352:Drug Resistance, Microbial; D004797:Enzyme-Linked Immunosorbent Assay; D006115:Greece; D006801:Humans; D007223:Infant; D008585:Meningitis, Meningococcal / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D009345:Neisseria meningitidis / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D012703:Serotyping; D013030:Spain
chemical_list: D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial