title: PCR-analyzed microsatellites of the mouse genome--additional polymorphisms among ten inbred mouse strains.
abstract: Eighty sequences from the mouse genome database containing microsatellites (simple sequence repeats) have been analyzed for size variation among ten different inbred strains of mice; 62/80 (77.5%) showed polymorphism of at least three alleles. We have been able to detect all the polymorphisms by agarose gel electrophoresis, often running the gels for up to 3 h. Between individual pairs of mouse strains to be used in chromosomal mapping studies in our laboratory, 35-60% polymorphism occurred. There are potentially enough microsatellites within the mouse and human genome to have a marker at every 1-cM distance. This simple approach will, therefore, continue to be useful in genome mapping studies, leading eventually to high-resolution maps of both the mouse and human genomes; this should allow for physical mapping and cloning of specific genes.
authors.affiliation: Transplantation Biology Section, MRC Clinical Research Centre, Harrow, Middlesex, UK.
authors.forename: G A
authors.initials: GA
authors.lastname: Fowlis
authors.forename: S
authors.initials: S
authors.lastname: Adelman
authors.forename: A M
authors.initials: AM
authors.lastname: Knight
authors.forename: E
authors.initials: E
authors.lastname: Simpson
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*
chemical_list: D004276:DNA, Satellite