Demonstration by in situ hybridization of the proopiomelanocortin gene in the rat heart.
A biotinylated oligonucleotide probe was used to demonstrate the presence in the heart of the portion of the proopiomelanocortin messenger RNA which contains the sequence for beta-endorphin. The probe indicated the presence of beta-endorphin messenger RNA in cardiac tissue and specifically in the cardiac muscle cell. The probe also confirmed the well-documented presence of messenger RNA for beta-endorphin in the anterior and neurointermediate lobes of the pituitary. These findings indicate that in addition to the pituitary, beta-endorphin is produced in situ in the heart.
Forman
L J
LJ
University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Department of Medicine, Camden 08103.
Bagasra
O
O
D000818:Animals; D001483:Base Sequence; D066298:In Vitro Techniques; D008297:Male; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010902:Pituitary Gland / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D001615:beta-Endorphin / Q000096:biosynthesis
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D001615:beta-Endorphin; D011333:Pro-Opiomelanocortin