Interferon-gamma reduces the thyroid peroxidase content of cultured human thyrocytes and inhibits its increase induced by thyrotropin.
To clarify the role of interferon-gamma (IFN gamma) in autoimmune thyroid diseases, we investigated the effects of IFN gamma on the content of thyroid peroxidase (TPO) and the expression of HLA-DR antigens in cultured normal human thyrocytes. The effect of TSH on the action of IFN gamma was investigated. Immunofluorescence staining and photometric analysis showed that IFN gamma not only induced the expression of DR antigen, but also reduced the content of TPO in a concentration-dependent manner. The addition of TSH increased the content of TPO and enhanced the IFN gamma-induced expression of DR antigen. IFN gamma also inhibited the increase in TPO content induced by TSH. Thus, complex interactions appear to exist between IFN gamma and TSH or thyroid-stimulating antibodies in the modulation of hormone secretion and autoimmune phenomena in the thyroid.
Asakawa
H
H
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Hanafusa
T
T
Kobayashi
T
T
Takai
S
S
Kono
N
N
Tarui
S
S
D000236:Adenoma / Q000201:enzymology / Q000601:surgery; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D005260:Female; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007453:Iodide Peroxidase / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins; D013961:Thyroid Gland / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D013964:Thyroid Neoplasms / Q000201:enzymology / Q000601:surgery; D013965:Thyroidectomy; D013972:Thyrotropin / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma; D013972:Thyrotropin; D007453:Iodide Peroxidase