Production of interleukin 8 by cultured synovial cells in response to interleukin 1 and tumor necrosis factor.
Both interleukin 1 alpha (IL-1 alpha) and tumor necrosis factor alpha (TNF alpha) stimulated the production of interleukin 8 (IL-8) by synovial cells in time and dose dependent manners. Enhanced chemotactic activity of polymorphonuclear cells (PMN) in culture supernatants of synovial cells was neutralized with anti-IL-8 antibody, thus showing synovial cells to be capable of secreting IL-8 which may contribute to PMN accumulation in rheumatoid inflamed joints.
Hirota
K
K
Department of Internal Medicine, Kitasato University School of Medicine, Kanagawa, Japan.
Akahoshi
T
T
Endo
H
H
Kondo
H
H
Kashiwazaki
S
S
D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D016209:Interleukin-8 / Q000032:analysis / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D011863:Radioimmunoassay; D013583:Synovial Membrane / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D007375:Interleukin-1; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D003609:Dactinomycin; D003513:Cycloheximide