Detection of donor-reactive alloantibody in the early posttransplant period. Elimination of therapeutic ALG as a complicating factor.
We have developed an immunoabsorbent reagent that can differentially remove ALG from human serum samples in vitro--i.e., goat antihorse IgG covalently linked to Sepharose beads. When used according to protocol, this immunoabsorbent can effectively remove up to 0.78 mg/ml of ALG from human serum mixtures. To demonstrate that immunoabsorption is selective, an HLA-B7-specific alloserum was mixed with a known amount of ALG and absorbed with antibody-conjugated Sepharose beads. The addition of ALG to the serum sample caused high degrees of nonspecific lympholysis in standard microcytotoxicity assays. When this serum/ALG mixture was immunoabsorbed detectable ALG activity was lost but the original HLA alloantibody titer (1:2) against specific target lymphocytes was retained.
Adams
P W
PW
Department of Surgery, Ohio State University College of Medicine, Columbus 43210.
Pagel
E
E
Orosz
C G
CG
D000918:Antibody Specificity; D000961:Antilymphocyte Serum; D006801:Humans; D007163:Immunosorbent Techniques; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000032:analysis*; D016377:Organ Transplantation / Q000502:physiology*; D014019:Tissue Donors
D000961:Antilymphocyte Serum; D007202:Indicators and Reagents; D007518:Isoantibodies