[Ultrasensitive immunoassay of hormones].
Two methods for ultrasensitive immunoassay of peptide hormones are presented. One is to reduce the nonspecific binding of labeled reactants in two-site immunoassay by transfer of immune complexes containing labeled reactants from one solid phase to another. The other is a novel noncompetitive immunoassay method for small peptides, in which peptides are biotinylated and subsequently measured by two-site assay using anti-peptide antibody and avidin (streptavidin).
Ishikawa
E
E
Department of Biochemistry, Medical College of Miyazaki.
D000818:Animals; D001360:Avidin; D001710:Biotin; D006728:Hormones / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D010455:Peptides / Q000032:analysis*
D006728:Hormones; D010455:Peptides; D001360:Avidin; D001710:Biotin