Helicobacter pylori lipopolysaccharide effect on the synthesis and secretion of gastric sulfomucin.
The effect of H. pylori lipopolysaccharide on the synthesis and secretion of sulfated mucin in gastric mucosa was investigated using mucosal segments incubated in the presence of [3H]proline, [3H]glucosamine and [35S]Na2SO4. The lipopolysaccharide, while showing no discernible effect on the apomucin synthesis was found to inhibit the process of mucin glycosylation and sulfation, which at 100 micrograms/ml lipopolysaccharide reached the optimal inhibition of 65%. The analysis of mucin secretory responses revealed that the lipopolysaccharide by first 15 min caused a 57% stimulation in sulfomucin secretion followed thereafter by inhibition, which reached maximum of 32% by 45 min. The results suggest that colonization of gastric mucosa by H. pylori may be detrimental to the process of gastric sulfomucin synthesis and secretion.
Liau
Y H
YH
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103.
Lopez
R A
RA
Slomiany
A
A
Slomiany
B L
BL
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism; D006031:Glycosylation; D016480:Helicobacter pylori; D007700:Kinetics; D008070:Lipopolysaccharides; D009077:Mucins / Q000096:biosynthesis* / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D051381:Rats; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014316:Tritium
D008070:Lipopolysaccharides; D009077:Mucins; D013431:Sulfates; D013462:Sulfur Radioisotopes; C012036:sodium sulfate; D014316:Tritium; D011392:Proline; D005944:Glucosamine