20-Hydroxyeicosatetraenoic acid is excreted as a glucuronide conjugate in human urine.
20-hydroxyeicosatetraenoic acid, a major renal P-450 metabolite of arachidonic acid, has been quantified in human urine using capillary gas chromatography/electron capture negative ion chemical ionization mass spectrometry. The urinary excretion of 20-hydroxyeicosatetraenoic acid was in the low pg/ml range. However, treatment of urine with beta-glucuronidase resulted in a 13- to 28-fold increase in its concentration. This suggests 20-hydroxyeicosatetraenoic acid differs from other eicosanoids in that it is excreted primarily as a glucuronide conjugate.
Prakash
C
C
Department of Pharmacology, Vanderbilt University, Nashville, TN 37232.
Zhang
J Y
JY
Falck
J R
JR
Chauhan
K
K
Blair
I A
IA
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005965:Glucuronates / Q000652:urine*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000652:urine*
D005965:Glucuronates; D006893:Hydroxyeicosatetraenoic Acids; C055987:20-hydroxy-5,8,11,14-eicosatetraenoic acid