Restin: a novel intermediate filament-associated protein highly expressed in the Reed-Sternberg cells of Hodgkin's disease.
We have identified a cDNA coding for a protein of 160 kDa which is expressed in in vitro cultured human peripheral blood monocytes. The predicted amino acid sequence contains an alpha-helical rod domain possessing features characteristic of intermediate filament proteins. However, the immunocytochemical staining pattern, abundance and solubility in Triton X-100/high salt buffers suggest that this protein is probably only associated with the intermediate filament network and represents a new type of intermediate filament associated protein. In a survey of normal, inflammatory and human tumour tissue samples, this protein, which we have named restin, was found to be highly expressed in Reed-Sternberg cells, the tumoral cells diagnostic for Hodgkin's disease. We suggest that restin overexpression may be a contributing factor in the progression of Hodgkin's disease.
Bilbe
G
G
Biotechnology, Pharma Division, Ciba-Geigy, Basel, Switzerland.
Delabie
J
J
Brüggen
J
J
Richener
H
H
Asselbergs
F A
FA
Cerletti
N
N
Sorg
C
C
Odink
K
K
Tarcsay
L
L
Wiesendanger
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D006689:Hodgkin Disease / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D016539:Reed-Sternberg Cells / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014746:Vimentin / Q000032:analysis
D004273:DNA, Neoplasm; D007381:Intermediate Filament Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D014746:Vimentin; C076744:cytoplasmic linker protein 170