title: Identification of a ROB-1 beta-lactamase in Haemophilus ducreyi.
abstract: A collection of 100 clinical isolates of Haemophilus ducreyi from Thailand were all found to harbor a 5.4-kb plasmid, designated pTH126, which was shown to contain the bla ROB-1 gene. Restriction enzyme analysis and DNA-DNA hybridization studies confirmed that pTH126 was similar to the ROB-1 beta-lactamase plasmid pVM105 from Actinobacillus pleuropneumoniae. In approximately one-half of the isolates, pTH126 was found together with pHD131, which mediates TEM-1 beta-lactamase production.
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
authors.forename: I W
authors.initials: IW
authors.lastname: Maclean
authors.forename: L
authors.initials: L
authors.lastname: Slaney
authors.forename: J M
authors.initials: JM
authors.lastname: Juteau
authors.forename: R C
authors.initials: RC
authors.lastname: Levesque
authors.forename: W L
authors.initials: WL
authors.lastname: Albritton
authors.forename: A R
authors.initials: AR
authors.lastname: Ronald
mesh_terms: D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D006191:Haemophilus ducreyi / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D007525:Isoelectric Focusing; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping; D013785:Thailand; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D004269:DNA, Bacterial; C071165:beta-lactamse ROB-1; D001618:beta-Lactamases