Evaluation of a monoclonal antibody, BC-1, which identifies an antigen expressed on the surface membrane of human extravillous trophoblast.
This paper describes a new Mab BC-1 which is directed at a cell surface antigen expressed by extravillous cytotrophoblast and not by villous cytotrophoblast, so it is useful for distinguishing between the two populations. The antigen recognised by BC-1 is trypsin-resistant, which allows it to be used for flow cytometric analysis of living trophoblast isolated by enzymic disaggregation. However, BC-1 is not trophoblast-specific but cross-reacts with some other tissues, in particular endothelial cells and peripheral blood monocytes. The nature of this antigen has not been established.
Loke
Y W
YW
Department of Pathology, University of Cambridge, United Kingdom.
Hsi
B L
BL
Bulmer
J N
JN
Grivaux
C
C
Hawley
S
S
Gardner
L
L
King
A
A
Carter
N P
NP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015415:Biomarkers; D002478:Cells, Cultured; D003429:Cross Reactions; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D009928:Organ Specificity; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014327:Trophoblasts / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015415:Biomarkers