Sequencing of cyclodepsipeptides (destruxins) using positive fast atom bombardment desorption tandem mass spectrometry.
The development of fast atom bombardment tandem mass spectrometry methodology is applied to the sequencing of the destruxin toxins (cyclodepsipeptides) which are 'wrong' cyclopeptides. The strategy is discussed in detail. Possible ways to overcome problems related to the resolution of isobaric fragment ions using deuterated compounds and the distinction between sequence and retrosequence are examined.
Lange
C
C
Laboratoire de Chimie Organique Structurale, UA CNRS 455, Université P. et M. Curie, Paris, France.
Mulheim
C
C
Vey
A
A
Pais
M
M
D000595:Amino Acid Sequence; D047630:Depsipeptides; D005656:Fungal Proteins; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D047630:Depsipeptides; D005656:Fungal Proteins; D010456:Peptides, Cyclic; C040423:destruxin A; C040425:destruxin E