Detection of Helicobacter pylori-like organisms in the stomach of some food-source animals using a monoclonal antibody.
To investigate a possible animal reservoir of Helicobacter pylori, 15 pigs, 15 rabbits and 5 cows slaughtered for consumption were studied. Raised serum IgG levels were found in 93% of the pigs and 87% of the rabbits, but levels were normal in the cattle. In the controlled testings three of 22 humans had elevated IgG to H pylori and in all three H pylori was detected by the use of monoclonal antibody. Helicobacter pylori were identified in gastric brushings by a monoclonal antibody in 8 out of 10 pigs and 7 out of 10 rabbits. This study suggests an animal reservoir of Helicobacter pylori which may be of importance in human infection.
Vaira
D
D
I Clinica Medica, Ospedale S Orsola, Università di Bologna, Italy.
Ferron
P
P
Negrini
R
R
Cavazzini
L
L
Holton
J
J
Ainley
C
C
Londei
M
M
Vergura
M
M
Dei
R
R
Colecchia
A
A
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D016123:Campylobacter jejuni / Q000276:immunology / Q000302:isolation & purification; D002417:Cattle / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005516:Food Microbiology; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D011817:Rabbits / Q000382:microbiology*; D013270:Stomach / Q000382:microbiology*; D013279:Stomach, Ruminant / Q000382:microbiology*; D013552:Swine / Q000382:microbiology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G