title: The eukaryotic translation initiation factor 4E is not modified during the course of vaccinia virus replication.
abstract: The ability of vaccinia virus to inhibit processes of cap-dependent translational initiation by inactivating the eukaryotic translation initiation factor 4E (eIF-4E) has been examined. Analyses of the quantities of eIF-4E present in either uninfected mouse L929 cells or vaccinia virus-infected cells showed that during the first 12 hr of virus replication, when there is a marked decrease in host gene expression in infected cells, there is no change in the total amount of eIF-4E present. Analyses of eIF-4E that was metabolically labeled with [32P] and then purified by affinity chromatography using m7GTP-Sepharose 4B, indicated that neither the incorporation of radiolabel into eIF-4E nor the amounts of eIF-4E capable of binding to cap structures changed significantly during virus replication. Immunodetection of phosphorylated and unphosphorylated eIF-4E in cell lysates fractionated by two-dimensional gel electrophoresis showed that the steady-state levels of phosphorylated and unphosphorylated forms of eIF-4E were similar in uninfected and virus-infected cells. These results suggest that vaccinia virus does not gain preferential translation of viral mRNAs over other mRNAs in the cell by reducing either eIF-4E phosphorylation or its ability to bind to the cap structure.
authors.affiliation: Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: T M
authors.initials: TM
authors.lastname: Gierman
authors.forename: R M
authors.initials: RM
authors.lastname: Frederickson
authors.forename: N
authors.initials: N
authors.lastname: Sonenberg
authors.forename: D J
authors.initials: DJ
authors.lastname: Pickup
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D039561:Eukaryotic Initiation Factor-4E; D015967:Gene Expression Regulation, Viral; D066298:In Vitro Techniques; D007739:L Cells; D051379:Mice; D010448:Peptide Initiation Factors / Q000378:metabolism*; D010766:Phosphorylation; D012315:RNA Caps / Q000378:metabolism; D014616:Vaccinia virus / Q000235:genetics*; D014779:Virus Replication
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D012315:RNA Caps