A simple method for the extraction of baculovirus DNA.
We have developed a method using lysozyme for DNA extraction from Baculoviruses using as a model Lymantria dispar nuclear polyhedrosis virus (LdNPV) obtained from infected larvae. This method proved to be quick, inexpensive and the extracted DNA was successfully used in molecular hybridization experiments.
Desmarteaux
D
D
Université du Québec à Trois-Rivières, Département de Chimie-Biologie, Canada.
Charpentier
G
G
Arella
M
M
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D004279:DNA, Viral / Q000302:isolation & purification*; D019286:Endopeptidase K; D007814:Larva / Q000382:microbiology; D009036:Moths / Q000382:microbiology; D009113:Muramidase; D012697:Serine Endopeptidases
D004279:DNA, Viral; D009113:Muramidase; D012697:Serine Endopeptidases; D019286:Endopeptidase K