Intracellular localization of the HLA class II gene products in transfected mouse L cells.
To investigate the impaired cell surface expression of human major histocompatibility antigen (HLA) in transfected L cells, we examined their intracellular localization by immunocytochemistry. HLA class II molecules produced in transfected L cells were mainly detected in the intracellular vesicles and in the nuclear envelope as granular precipitates. The results suggest that the intracellular transport of the newly synthesized molecules in transfected L cells is impaired at some point along the pathway from the rough endoplasmic reticulum (RER) to the medial-, trans-Golgi apparatus.
Nakatsuji
T
T
Department of Transplantation, Tokai University School of Medicine, Isehara, Japan.
Akatsuka
A
A
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000648:ultrastructure*; D006681:HLA-D Antigens / Q000096:biosynthesis* / Q000235:genetics; D006684:HLA-DR Antigens / Q000096:biosynthesis; D007150:Immunohistochemistry; D007739:L Cells / Q000276:immunology / Q000648:ultrastructure*; D051379:Mice; D015388:Organelles / Q000276:immunology; D014162:Transfection / Q000235:genetics
D006681:HLA-D Antigens; D006684:HLA-DR Antigens