title: Renal dehydropeptidase-I stability of LJC 10,627, a new carbapenem antibiotic.
abstract: LJC 10,627 is a new parenteral carbapenem antibiotic. LJC 10,627 stability against human renal dehydropeptidase-I was compared with that of imipenem. Hydrolysis of this compound was not detectable by spectrophotometrical assay. Even after a 2-h incubation of antibiotics with this enzyme at 30 degrees C, the concentration of LJC 10,627 remained at 92.3% of the initial concentration, whereas imipenem completely disappeared. Thus, it was found that this compound was highly stable against human renal dehydropeptidase-I. Furthermore, LJC 10,627 had a low affinity for this enzyme, as indicated by the high Ki value (0.38 mM).
authors.affiliation: Biological Research Laboratories, Lederle Ltd., Saitama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hikida
authors.forename: K
authors.initials: K
authors.lastname: Kawashima
authors.forename: K
authors.initials: K
authors.lastname: Nishiki
authors.forename: Y
authors.initials: Y
authors.lastname: Furukawa
authors.forename: K
authors.initials: K
authors.lastname: Nishizawa
authors.forename: I
authors.initials: I
authors.lastname: Saito
authors.forename: S
authors.initials: S
authors.lastname: Kuwao
mesh_terms: D000818:Animals; D015780:Carbapenems / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004150:Dipeptidases / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D015378:Imipenem / Q000378:metabolism; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008252:Macaca fascicularis; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D013845:Thienamycins
chemical_list: D015780:Carbapenems; D013845:Thienamycins; D015378:Imipenem; D004150:Dipeptidases; C038937:dipeptidase; C065257:biapenem