Cephaloridine nephrotoxicity is potentiated by selenium deficiency but not copper deficiency in rats.
Lipid peroxidation may contribute to the nephrotoxicity of cephaloridine, a beta-lactam antibiotic. Copper and Se may protect against free radical damage, and dietary Se deficiency potentiates cephaloridine nephrotoxicity. The objectives of this study were to further investigate potentiation of cephaloridine toxicity by Se deficiency and to determine whether Cu deficiency increases cephaloridine-induced injury. Weanling male Sprague-Dawley rats were fed adequate, Cu-deficient, Se-deficient, and Se and Cu-deficient diets for 4 wk and subsequently injected i.p. with cephaloridine (1200 mg/kg body wt) or saline. Nephrotoxic response to cephaloridine occurred, with increased plasma urea, kidney weight, excretion of urinary enzymes, and kidney lesions. Cephaloridine also increased plasma sorbitol dehydrogenase activity. Selenium deficiency depressed kidney glutathione peroxidase activity (78%) and potentiated cephaloridine nephrotoxicity. Copper deficiency did not increase cephaloridine nephrotoxicity; the small depression (13%) in kidney Cu,Zn-superoxide dismutase activity may not have been sufficient to impair antioxidant status. However, the marked depression in kidney glutathione peroxidase activity during Se deficiency may have impaired antioxidant status and enhanced cephaloridine-induced injury. In contrast to results in the kidney, neither Se deficiency nor Cu deficiency potentiated cephaloridine hepatotoxicity, as assessed by plasma SDH activity.
Kays
S E
SE
Department of Foods and Nutrition, College of Family and Consumer Sciences, University of Georgia, Athens 30602.
Crowell
W A
WA
Johnson
M A
MA
D000818:Animals; D002509:Cephaloridine / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury; D003300:Copper / Q000008:administration & dosage / Q000172:deficiency*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000201:enzymology / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D007064:L-Iditol 2-Dehydrogenase / Q000097:blood; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology; D008297:Male; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000008:administration & dosage / Q000172:deficiency*; D013482:Superoxide Dismutase / Q000378:metabolism; D014508:Urea / Q000097:blood
D003300:Copper; D014508:Urea; D007064:L-Iditol 2-Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium; D002509:Cephaloridine