The relation of ligand binding to redox state in the hexa-heme nitrite reductase of Wolinella succinogenes.
Ligand binding reactions and the relation between redox state and ligand binding in the hexa-heme nitrite reductase of Wolinella succinogenes have been studied using laser flash photolysis. On a picosecond time scale, a rapid excursion was observed corresponding to the breaking and reforming of an iron histidine bond. With the CO derivative, a geminate reaction was observed with a rate of 3 ns-1. On a nanosecond time scale, no slower geminate reactions were observed. For the cyanide derivative, no geminate reactions were observed at either time scale. The second order reaction of CO with the enzyme had a time course consisting of two distinct components. This time course changed in form as the enzyme came to equilibrium with CO, and the slower rebinding component was replaced by a faster rebinding component. It is suggested that CO binding enhances reduction of a heme with an unusually low redox potential and opens the structure of the active site to allow a faster second order reaction of CO. The proportion of the geminate CO reaction was unchanged, consistent with changes relatively remote from the ligand binding site. The second order reactions of cyanide also showed that redox effects influence its rebinding reaction. Adding cyanide to the CO complex of nitrite reductase showed that the two ligands have distinct heme binding sites.
Blackmore
R S
RS
Department of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.
Gibson
Q H
QH
Greenwood
C
C
D001665:Binding Sites; D002248:Carbon Monoxide / Q000378:metabolism; D006418:Heme / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D009572:Nitrite Reductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010782:Photolysis; D013057:Spectrum Analysis; D016968:Wolinella / Q000201:enzymology*
D008024:Ligands; D006418:Heme; D002248:Carbon Monoxide; D009572:Nitrite Reductases