Relationship of single-stranded DNA-binding proteins of Ehrlich ascites tumour to cell growth phase and DNA replications.
The possible involvement of SSB-proteins in DNA replication in Ehrlich ascites tumour (EAT) has been investigated. A direct relation (the computer-generated correlation coefficient was 0.9) between the SSB-proteins content in chromatin and intensity of the replicative synthesis of DNA in various preparation of EAT in vivo and in vitro is observed. Addition of exogenous SSB-proteins to the permeable EAT cells has been found to increase the replicative synthesis. Although eukaryotic SSB-proteins are not complete analogs of the prokaryotic SSB-proteins, they evidently participate in DNA replication in eukaryotic cells and possibly are intracellular regulators of proliferation.
Koterov
A N
AN
Institute of Biophysics, USSR Ministry of Public Health, Moscow.
Novoradovskaya
N A
NA
Filippovich
I V
IV
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D002455:Cell Division; D004261:DNA Replication / Q000187:drug effects; D004277:DNA, Single-Stranded / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D008297:Male; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics* / Q000502:physiology
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins