Activity of glutathione-S-transferase in rat liver and kidneys after administration of lead or cadmium.
The influence of an acute dose of lead nitrate or cadmium chloride on the activity of glutathione-S-transferase (GST) was investigated in rats. CdCl2 (10 mumol/kg) did not influence the enzyme activity in either organ. In contrast Pb(NO3)2 (100 mumol/kg) caused a significant increase of GST activity in both organs. SDS polyacrylamide gel electrophoresis (SDS-PAGE) showed that in liver the activity enhancement is due mainly to the induction of the isoenzyme GST 7-7, while in the kidneys the activity of all the isoenzymes is increased.
Planas-Bohne
F
F
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, Germany.
Elizalde
M
M
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D004137:Dinitrochlorobenzene / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D007668:Kidney / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D007854:Lead / Q000633:toxicity*; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D004137:Dinitrochlorobenzene; D007527:Isoenzymes; D002104:Cadmium; D007854:Lead; D005982:Glutathione Transferase