title: Purification and characterization of PSP-I and PSP-II, two major proteins from porcine seminal plasma.
abstract: Two major glycoproteins, designated PSP-I and PSP-II, were purified from porcine seminal plasma by ammonium sulfate fractionation, CM-cellulose chromatography, gel filtration on Sephadex G-75 (superfine), and reverse phase high performance liquid chromatography. These two proteins exist in several forms differing mainly in the carbohydrate moiety. The complete amino acid sequence of PSP-I has been determined by automated Edman degradation of peptides generated by proteolytic digestion and cyanogen bromide cleavage. The protein is 109 residues long and has a single glycosylation site at the asparagine residue at position 47. In addition, the N-terminal sequence of PSP-II has also been determined. PSP-I is a unique protein; a sequence homology search using the protein data base did not reveal any significant homology with other proteins. PSP-II shares 50% sequence homology with a family of zona pellucida-binding glycoproteins at the N-terminus.
authors.affiliation: Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California 91010-0269.
authors.forename: K J
authors.initials: KJ
authors.lastname: Rutherfurd
authors.forename: K M
authors.initials: KM
authors.lastname: Swiderek
authors.forename: C B
authors.initials: CB
authors.lastname: Green
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: J E
authors.initials: JE
authors.lastname: Shively
authors.forename: S C
authors.initials: SC
authors.lastname: Kwok
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D006868:Hydrolysis; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012661:Semen / Q000378:metabolism*; D029589:Seminal Plasma Proteins; D029607:Seminal Vesicle Secretory Proteins; D013552:Swine
chemical_list: D000596:Amino Acids; D006023:Glycoproteins; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; D029607:Seminal Vesicle Secretory Proteins; C043076:beta-microseminoprotein; C074521:seminal vesicle secretory protein II, porcine; C074520:seminal vesicle secretory protein 109, porcine