title: [Origin of secretions after denervation of the small intestine in the dog].
abstract: The origin of the severe diarrhea appearing after intestinal denervation or transplantation was studied on the 3rd and 14th postoperative days in 5 groups of dogs undergoing total or partial denervation. The net movements of water and electrolytes were investigated by employing an experimental model of intestinal perfusion in isolated loops in vivo. The active uptake of phenylalanine and beta-methylglucoside and Na(+)-K(+)-ATPase activity were used for in-vitro evaluation. Bacteriologic and histological specimens were also taken. Following total denervation with anastomosis, a considerable loss of water and electrolytes as well as numerous E. coli were found in the entire small intestine. This net secretion is due to the stagnation of bacteria in the presence of complete denervation and the absence of food since the animals in this group could not eat properly following general anesthesia and surgery. Consequently, peristalsis was not stimulated and bacterial overgrowth occurred. In the group denervation with pseudo-anastomosis, perfusion studies showed a decrease of absorption in the jejunum and minimal but significant secretion in the ileum. A high number of E. coli was also present. Since the mucosa remained intact, nutrition per os was resumed in the immediate postoperative period and excessive water and electrolyte loss was avoided. The high number of bacteria was due to a decrease in intestinal motility since complete denervation was performed. In the denervation group, water and electrolyte movements were identical to those observed in the preceding group but the entire intestine remained sterile.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division de chirurgie expérimentale, CHUV, Lausanne.
authors.forename: M
authors.initials: M
authors.lastname: Maccari
authors.forename: B
authors.initials: B
authors.lastname: Winistörfer
authors.forename: V
authors.initials: V
authors.lastname: Mirkovitch
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D003714:Denervation; D003967:Diarrhea / Q000382:microbiology / Q000503:physiopathology*; D004285:Dogs; D007419:Intestinal Secretions / Q000502:physiology*; D007421:Intestine, Small / Q000294:innervation* / Q000382:microbiology; D010528:Peristalsis / Q000502:physiology; D011183:Postoperative Complications / Q000382:microbiology / Q000503:physiopathology*
vernacular_title: L'origine de la sécrétion après la dénervation de l'intestin grêle de chien.