The mechanism of photosensitization in photodynamic therapy: chemiluminescence caused by photosensitization of porphyrins in saline containing human serum albumin.
Chemiluminescence (CL) caused by photosensitization of porphyrins in phosphate buffered saline (PBS) solution containing 3% human serum albumin (HSA) was observed for the first time. Irrespective of porphyrins concerned, CL shows a spectrum ranging from 380 to 520 nm with a peak near 450 nm and decays almost single-exponentially with a lifetime of about 15 s. The intensity of CL depends on concentrations of porphyrins and HSA in PBS solution. We have examined a number of porphyrins and observed CL for the compounds with triplet lifetimes longer than 0.1 ms. The appearance and quenching of CL by photosensitization of porphyrin-HSA systems indicate that type II reaction by singlet oxygen occurs significantly in photodynamic therapy resulting in hypoxic regions in environments surrounding the sensitizer.
Takemura
T
T
Research Institute of Applied Electricity, Hokkaido University, Sapporo, Japan.
Ohta
N
N
Nakajima
S
S
Sakata
I
I
D006801:Humans; D049449:Luminescence; D008954:Models, Biological; D010778:Photochemotherapy; D011166:Porphyrins / Q000494:pharmacology*; D012709:Serum Albumin / Q000187:drug effects* / Q000528:radiation effects
D011166:Porphyrins; D012709:Serum Albumin