Determination of oxytetracycline in blood serum by high-performance liquid chromatography with direct injection.
A direct injection analysis by high-performance liquid chromatography has been developed for oxytetracycline in serum of animals and fish. A Hisep shielded hydrophobic phase column (15 cm x 4.6 mm I.D.) and a mobile phase of methanol-0.2 M oxalic acid (10:90, v/v, pH 7.0) with ultraviolet detection at 360 nm were used. The standard calibration curves in serum of chicken, hog, cattle and rainbow trout were linear over the range 0.1-20 micrograms/ml. The recoveries of oxytetracycline from all serum samples determined at two different concentrations (0.5 and 2.0 micrograms/ml) were 88-103%. The detection limit was 0.05 micrograms/ml for every serum sample.
Ueno
R
R
Faculty of Bioresources, Mie University, Japan.
Uno
K
K
Aoki
T
T
D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D010118:Oxytetracycline / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D014337:Trout
D010118:Oxytetracycline