Two antigens detected on human ocular melanomas with the mouse monoclonal antibodies 2/10SN and 10/12SN.
Seven human ocular melanoma cell lines were established in vitro and 3 of these, GU-4, LLN-40 and its subline C17-11, were characterized. Mice were immunized with these ocular melanoma cell lines, and 2 hybridomas producing monoclonal IgG1 antibodies (MAb) were produced. MAb 2/10SN recognizes a 44-kDa monomeric protein, whereas MAb 10/12SN reacts with an 83/65-kDa heterodimeric protein. These melanoma-associated antigens (MAA) are detected at high concentrations in the cytoplasm of ocular melanoma cells. However, cell-surface labelling techniques suggest that these MAA are also associated with the cell-surface membrane. These 2 ocular MAA are also expressed by several skin melanoma cell lines. Immunohistochemical studies have localized these antigens to ocular and skin melanomas, to sweat ducts and basal squamous cells in normal skin, with limited expression in several other normal tissues and some carcinomas. Biodistribution studies in nude mice with human ocular melanomas have demonstrated good localization of 125I-labeled MAb 2/10SN at the tumor sites. Therefore, these 2 MAbs, 2/10SN and 10/12SN, recognize MAA which appear to be unique and may prove useful for imaging purposes.
Safi
N
N
Department of Radiology, George Washington University Medical Center, Washington, D.C. 20037.
Kantor
R R
RR
Atkinson
B
B
Moratz
C
C
Wilt
A R
AR
Pancake
S
S
Reba
R
R
Mathieson
B J
BJ
Desgrez
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000737:chemistry; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D005134:Eye Neoplasms / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D046911:Macromolecular Substances; D008545:Melanoma / Q000276:immunology*; D008546:Melanoma, Experimental / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D011863:Radioimmunoassay; D012878:Skin Neoplasms / Q000276:immunology; D014407:Tumor Cells, Cultured; D014604:Uveal Neoplasms / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D046911:Macromolecular Substances