Exposure of HIV-infected cells to phospholipid leads to membrane alterations and selective growth retardation.
The effect of exogenous phosphatidylcholine on structure and function of plasma membranes from HIV-1-producing cells and from their non-infected counterparts was determined. The membrane protein composition was not affected by phospholipid treatment. Membrane fluidity and Ca(2+)-permeability were increased in virus-producing cells and in control cells after lipid treatment. The triacylglycerol content of the plasma membranes was increased in virus-producing cells after lipid treatment, whereas the content of phospholipid and cholesterol was not changed. The increased triacylglycerol content was in accordance with a relatively higher rate of [14C]oleic acid incorporation into triacylglycerols of the virus-producing cells after lipid treatment as shown by metabolic labeling. The results suggest that a latent cytopathic effect of HIV-infection becomes manifest if the cells are exposed to exogenous phospholipid and this may open a way to preferentially eliminate HIV-producing cells.
Kerler
F
F
Abteilung für Zellchemie, GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg, Germany.
Hübner
C
C
Erfle
V
V
Buff
K
K
D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000382:microbiology; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000032:analysis; D015497:HIV-1 / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D010713:Phosphatidylcholines / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000032:analysis; D014779:Virus Replication / Q000187:drug effects*
D005227:Fatty Acids; D008563:Membrane Lipids; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D014763:Viral Matrix Proteins; D002118:Calcium