Lipid peroxidation and irreversible damage in the rat hepatocyte model. Protection by the silybin-phospholipid complex IdB 1016.
IdB 1016 is a new silybin-phospholipid complex which is more bioavailable than the flavonoid silybin itself and displays free radical scavenging and antioxidant properties in liver microsomes. We report here that the addition of increasing concentrations of IdB 1016 to isolated rat hepatocytes caused a dose-dependent inhibition of lipid peroxidation induced by ADP-Fe3+ or cumene hydroperoxide. Moreover, IdB 1016 at the concentration which completely prevented MDA formation also protected isolated hepatocytes against the toxicity of pro-oxidant agents such as allyl alcohol, cumene hydroperoxide and bromotrichloromethane, without interfering with the activation mechanism of these xenobiotics. Similar protection was also obtained in hepatocytes prepared from animals pretreated in vivo with IdB 1016 while rat supplementation with pure silybin was totally inefficient. These results indicate IdB 1016 as being a potentially useful protective agent against free radical-mediated toxic liver injury.
Carini
R
R
Department of Experimental Medicine and Oncology, University of Turin, Italy.
Comoglio
A
A
Albano
E
E
Poli
G
G
D000433:1-Propanol / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000633:toxicity; D001975:Bromotrichloromethane / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008954:Models, Biological; D010713:Phosphatidylcholines / Q000008:administration & dosage / Q000494:pharmacology*; D020005:Propanols; D051381:Rats; D011919:Rats, Inbred Strains; D012838:Silymarin / Q000008:administration & dosage / Q000494:pharmacology*
D000975:Antioxidants; D001555:Benzene Derivatives; D010713:Phosphatidylcholines; D020005:Propanols; D012838:Silymarin; C067772:IdB 1016; C006463:allyl alcohol; D008315:Malondialdehyde; D000433:1-Propanol; D001975:Bromotrichloromethane; C007164:cumene hydroperoxide