title: An interpretation of cell separation mechanism on polyamine graft copolymers.
abstract: Interaction between polyamide microcapsules having different balances of negative and positive charges on their surface, that is, different isoelectric points and copolymers with different numbers of polyamine macromer of a definite chain length grafted on poly(2-hydroxyethyl methacrylate) backbone was studied by measuring the amount adhered of the microcapsules onto the surface of copolymer-coated glass beads at different pH values. Maximum microcapsule adhesion was observed for a proper combination of microcapsule and copolymer to suggest that separation of a specified cell population from others on the surface of copolymer-coated glass beads can be explained in terms of preferential adhesion through electrostatic interaction of the specified cells with the copolymer.
authors.affiliation: Faculty of Pharmaceutical Sciences, Science University of Tokyo, Shinjuku-ku, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshida
authors.forename: N
authors.initials: N
authors.lastname: Muramatsu
authors.forename: K
authors.initials: K
authors.lastname: Kataoka
authors.forename: H
authors.initials: H
authors.lastname: Ohshima
authors.forename: T
authors.initials: T
authors.lastname: Kondo
mesh_terms: D002469:Cell Separation / Q000295:instrumentation*; D005898:Glass; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008863:Microspheres; D009994:Osmolar Concentration; D011073:Polyamines; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives*; D013499:Surface Properties
chemical_list: D011073:Polyamines; C056971:poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; D011102:Polyhydroxyethyl Methacrylate