Induction of R-bands on human chromosomes by TfiI as the consequence of local differences in target richness.
We present a new restriction enzyme-banding pattern on fixed human chromosomes. R-bands are induced by TfiI, an enzyme that cuts DNA at GA(A/T)TC, that is, at HinfI sites having A or T in the central position. Results suggest that regional differences in the frequency of targets are responsible for the effect caused by this enzyme, whereas conformational differences between G- and R-bands would not affect the enzyme action.
Tagarro
I
I
Departamento de Biología, Unidad de Genética, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
González-Aguilera
J J
JJ
Fernández-Peralta
A M
AM
D002871:Chromosome Banding / Q000379:methods*; D002877:Chromosomes, Human / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D009690:Nucleic Acid Conformation
C075307:endodeoxyribonuclease TfiI; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases