title: Glycosidase activities in gingival crevicular fluid in subjects with adult periodontitis or gingivitis.
abstract: Specific glycosidase activities were determined in samples of gingival crevicular fluid (GCF) collected from eight predetermined sites in two groups, each of 20 adult patients, with either gingivitis or periodontitis. The total activities (as units of enzyme activity per sample) of alpha-L-fucosidase, sialidase, beta-N-acetylglucosaminidase, beta-galactosidase, beta-glucosidase and alpha-glucosidase were significantly greater in the periodontitis group. In contrast, the total beta-mannosidase and hexosaminidase A activities were significantly greater in the gingivitis group, while there was no significant difference in the total alpha-mannosidase activity between the groups. Only the specific activities (as units of enzyme activity per min per microliter of GCF) of beta-mannosidase and hexosaminidase A were significantly different between the groups being greater in the gingivitis group. When used to predict the clinical status of individual periodontal sites, the total enzyme activities had specificity and sensitivity values of 91.9 and 61.3%, respectively. Measurement of glycosidase activities might thus have a role in monitoring the efficacy of periodontal treatment or in predicting future periodontal disease but this will require further investigation.
authors.affiliation: Hunterian Dental Research Unit, London Hospital Medical College, Whitechapel, U.K.
authors.forename: D
authors.initials: D
authors.lastname: Beighton
authors.forename: J R
authors.initials: JR
authors.lastname: Radford
authors.forename: M N
authors.initials: MN
authors.lastname: Naylor
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D002908:Chronic Disease; D004796:Clinical Enzyme Tests / Q000379:methods / Q000706:statistics & numerical data; D016002:Discriminant Analysis; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D005891:Gingivitis / Q000175:diagnosis*; D006026:Glycoside Hydrolases / Q000032:analysis*; D006801:Humans; D008875:Middle Aged; D010518:Periodontitis / Q000175:diagnosis*; D011379:Prognosis
chemical_list: D006026:Glycoside Hydrolases