The use of capillary electrophoresis to identify cationic proteins in human parotid saliva.
Eight proteins, HRPs 1, 2, 3, 4, 5 and 6, lysozyme and histatin 6, are the major cationic components of the parotid salivas of normal healthy individuals. Histatins 2 and 4 appear to be further degradation products of the HRPs. Capillary electrophoresis separates all of these eight components, thus allowing future studies to correlate protein concentration with antimicrobial activity in health and disease.
Lal
K
K
Department of Oral Biology and Pathology, State University of New York, Stony Brook 11794-8702.
Xu
L
L
Colburn
J
J
Hong
A L
AL
Pollock
J J
JJ
D000595:Amino Acid Sequence; D002412:Cations; D004586:Electrophoresis / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D009113:Muramidase / Q000032:analysis; D010306:Parotid Gland / Q000378:metabolism; D011506:Proteins / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D000099053:Histidine-Rich Glycoprotein
D002412:Cations; D006023:Glycoproteins; D011506:Proteins; D012471:Salivary Proteins and Peptides; D000099053:Histidine-Rich Glycoprotein; D009113:Muramidase