title: Effect of timing of administered calcium lactate on the sucrose-induced intraoral demineralization of bovine enamel.
abstract: A number of soluble calcium salts are known to reduce the demineralization of enamel in the mouth. The present study was undertaken to examine the effects of rinses containing different concentrations of calcium lactate, and the time of giving the rinses with respect to sucrose challenges. Subjects wore palatal appliances containing blocks of bovine enamel whose surfaces were covered with Streptococcus mutans IB 1600, and rinsed with 10% sucrose for 1 min. Changes in iodide penetrability of the enamel, and the pH and extracellular ion concentrations of the streptococcal plaque were determined. When added to the sucrose rinse, 100 or 150 mM calcium lactate reduced demineralization by about 35%, although the plaque pH was not affected. Plaque calcium was elevated but diffused away rapidly so that concentrations after 45 min were close to control values. Plaque inorganic phosphate and lactate were not affected. Ongoing demineralization appeared to be stopped when 100 mM calcium lactate was given 15 min after the sucrose rinse. When the lactate was given 15 min before the sucrose rinse, demineralization was reduced by only about 25%, consistent with the rapid diffusion of plaque calcium. The combination of (i) pretreatment with calcium lactate and (ii) admixture of calcium lactate with sucrose was most effective. Demineralization was reduced about 55% with 100 mM calcium lactate under these conditions, and protective effects were seen with as little as 25 mM. In summary, the findings demonstrate the enamel-protective effect of relatively low concentrations of calcium lactate, and point to the need to sustain a high plaque calcium during periods of maximum acidogenicity.
authors.affiliation: Department of Biochemistry, Forsyth Dental Center, Boston, MA 02115.
authors.forename: S
authors.initials: S
authors.lastname: Kashket
authors.forename: T
authors.initials: T
authors.lastname: Yaskell
mesh_terms: D000328:Adult; D000818:Animals; D002118:Calcium / Q000032:analysis; D002417:Cattle; D003743:Dental Enamel / Q000187:drug effects*; D003747:Dental Enamel Solubility / Q000187:drug effects; D003773:Dental Plaque / Q000737:chemistry / Q000382:microbiology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000008:administration & dosage / Q000032:analysis / Q000627:therapeutic use*; D019344:Lactic Acid; D008297:Male; D008875:Middle Aged; D009067:Mouthwashes; D010710:Phosphates / Q000032:analysis; D013295:Streptococcus mutans / Q000378:metabolism; D013395:Sucrose / Q000008:administration & dosage / Q000009:adverse effects*; D013997:Time Factors; D017001:Tooth Demineralization / Q000139:chemically induced / Q000517:prevention & control*
chemical_list: D007773:Lactates; D009067:Mouthwashes; D010710:Phosphates; D019344:Lactic Acid; D013395:Sucrose; D002118:Calcium