title: Picosecond dynamics of bacteriorhodopsin, probed by time-resolved infrared spectroscopy.
abstract: The photoinduced reaction cycle of bacteriorhodopsin (BR) has been studied by means of a recently developed picosecond infrared spectroscopic method at ambient temperature. BR - K difference spectra between 1560 and 1700 cm-1 have been recorded at delay times from 100 ps to 14 ns. The spectrum remains unchanged during this period. The negative difference OD band at 1660 cm-1 indicates the peptide backbone responds within 50 ps. A survey in the region of carboxylic side chain absorption around 1740 cm-1 reveals that perturbations of those groups, present in low-temperature FTIR spectra, are not observable within 10 ns, suggesting a slow conformational change.
authors.affiliation: Department of Chemistry, University of Pennsylvania, Philadelphia 19104.
authors.forename: R
authors.initials: R
authors.lastname: Diller
authors.forename: M
authors.initials: M
authors.lastname: Iannone
authors.forename: B R
authors.initials: BR
authors.lastname: Cowen
authors.forename: S
authors.initials: S
authors.lastname: Maiti
authors.forename: R A
authors.initials: RA
authors.lastname: Bogomolni
authors.forename: R M
authors.initials: RM
authors.lastname: Hochstrasser
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry*; D006217:Halobacterium; D007700:Kinetics; D010777:Photochemistry; D013055:Spectrophotometry, Infrared
chemical_list: D001436:Bacteriorhodopsins