Detection of cauliflower mosaic virus (CaMV) in single aphids by the polymerase chain reaction (PCR).
The detection for the first time of a plant virus in a single aphid by the high sensitivity polymerase chain reaction (PCR) technology is reported. The application of PCR for the detection of viruses in their vectors will aid the understanding of the complex virus-vector relationship and therefore allow the development of new approaches for control of spread of plant virus diseases.
López-Moya
J J
JJ
U.E.I. Fitopatologia, Centro de Investigaciones Biológicas (C.S.I.C.), Madrid, Spain.
Cubero
J
J
López-Abella
D
D
Díaz-Ruíz
J R
JR
D000818:Animals; D001042:Aphids / Q000737:chemistry / Q000235:genetics* / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis; D007303:Insect Vectors / Q000737:chemistry / Q000235:genetics* / Q000382:microbiology; D009029:Mosaic Viruses / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases; D016133:Polymerase Chain Reaction / Q000379:methods
D004279:DNA, Viral