title: Defective in vitro motility of polymorphonuclear leukocytes of homozygote and heterozygote Chediak-Higashi cats.
abstract: The in vitro migratory responses of neutrophils of homozygote and heterozygote Chediak-Higashi cats were defective in an under-agarose assay when compared to the behavior of phagocytes of control cats. The linear distances traversed by the leading front of migrating Chediak-Higashi neutrophils toward streptococcal culture supernatant, zymosan-activated serum or buffer were reduced and smaller numbers of Chediak-Higashi phagocytes populated the resulting migration areas than did cells of control animals. The relative migration parameters of the Chediak-Higashi phagocytes, however, did not differ from the corresponding parameters of control neutrophils in the presence of streptococcal culture supernatant. Therefore, phagocytes of homozygote and heterozygote Chediak-Higashi cats recognized and responded equally well to the bacterial stimuli as did cells of control animals but traveled shorter distances primarily because of a reduced inherent motility. Similar results were also obtained when the feline phagocytes were attracted by zymosan-activated serum. In addition the relative migration parameters of the neutrophils of homozygote Chediak-Higashi cats were reduced and the normalized spatial distributions of their migrating cells were significantly different in the presence of 100% and 20% zymosan-activated serum when compared to the corresponding migration parameters of carrier and control animals. Defective recognition or responses to the higher concentrations of these host-derived attractants complicated, therefore, the already reduced inherent motility of the phagocytes of homozygote Chediak-Higashi cats.
authors.affiliation: Department of Microbiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
authors.forename: S P
authors.initials: SP
authors.lastname: Colgan
authors.forename: A M
authors.initials: AM
authors.lastname: Blancquaert
authors.forename: M A
authors.initials: MA
authors.lastname: Thrall
authors.forename: W J
authors.initials: WJ
authors.lastname: Bruyninckx
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000235:genetics / Q000276:immunology*; D002415:Cats; D002464:Cell Migration Inhibition; D002609:Chediak-Higashi Syndrome / Q000235:genetics / Q000662:veterinary*; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D013293:Enterococcus faecalis / Q000276:immunology; D006579:Heterozygote; D006720:Homozygote; D009504:Neutrophils / Q000276:immunology; D010586:Phagocytes / Q000276:immunology; D015054:Zymosan / Q000276:immunology
chemical_list: D015054:Zymosan