title: Of orphons and UHOs. Delimitation of the germline repertoire of human immunoglobulin kappa genes.
abstract: Two problems in defining the germline repertoire of immunoglobulin kappa genes were investigated. One concerns putative transposed V kappa genes (orphons), the other one weak hybridization signals which may or may not turn out to be V kappa genes (UHOs). It was shown by sequencing that the three V kappa genes Z2, Z3 and Z4 are very closely related to the Z1 and V118 genes and to two other genes which had been localized on chromosomes 1 and 22, i.e. outside the kappa locus on chromosome 2. It is therefore likely that also the Z2-Z4 genes are orphons and not part of the kappa locus. Two UHOs turned out not to contain V kappa-like structures. This together with previous results makes it likely that we have detected all germline V kappa genes with the available hybridization probes.
authors.affiliation: Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie, Universität München.
authors.forename: F
authors.initials: F
authors.lastname: Röschenthaler
authors.forename: K F
authors.initials: KF
authors.lastname: Schäble
authors.forename: R
authors.initials: R
authors.lastname: Thiebe
authors.forename: H G
authors.initials: HG
authors.lastname: Zachau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D005803:Genes, Immunoglobulin; D005854:Germ Cells / Q000737:chemistry*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains