A method for cytogenetic analysis of boar spermatozoa using hamster oocytes.
In this paper, the authors detail a method for displaying boar spermatozoa chromosomes using heterospecific zona-free hamster oocyte penetration technique. Semen samples from two Large-White boars having a normal spermogram were studied. The first one had a normal karyotype (38,XY), the second carried a reciprocal translocation rcp(3;7)(p1,3;q2,1). After in vitro fertilization by capacitated sperm, culture and cytogenetic analysis of hamster eggs we obtained metaphase spreads of spermatozoa chromosomes. The ratio of X- and Y-bearing spermatozoa was 49.2% and 50.8%, respectively.
Benkhalifa
M
M
Laboratoire de Cytogénétique Médicale, Faculté de Médecine, Clermont-Ferrand, France.
Bonneau
M
M
Popescu
P
P
Boscher
J
J
Boucher
D
D
Malet
P
P
D000818:Animals; D006224:Cricetinae; D003582:Cytogenetics / Q000379:methods*; D005260:Female; D007621:Karyotyping / Q000662:veterinary; D008297:Male; D008647:Mesocricetus; D008677:Metaphase / Q000235:genetics; D009865:Oocytes / Q000502:physiology; D013075:Sperm Capacitation / Q000235:genetics; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D013480:Superovulation / Q000235:genetics; D013552:Swine / Q000235:genetics*