Allergens from rye pollen (Secale cereale). II. Characterization and partial purification.
Rye pollen was incubated for 30 min and proteins extracted at this time were collected as extract A (EA). The same pollen grains were resuspended in buffer and incubated for 18.5 h. Proteins extracted in this period were designated extract B (EB). Both extracts were subfractionated by DEAE ion-exchange chromatography and allergen presence in peaks detected by the dot-immunobinding technique. The results reveal that unretained proteins (peaks 1 and 2) and proteins eluted at 0.2 M NaCl from extract B contain the highest proportion of allergens. SDS-PAGE of chromatographic peaks showed that peak 2 from extract B contains a highly purified 28 kDa band. On the skin of allergic patients this band gave a stronger positive prick test than for the crude extract.
Montero
M T
MT
Research Department, Hospital Ramón y Cajal, Madrid, Spain.
Alonso
E
E
Sainz
T
T
D000485:Allergens / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D006801:Humans; D011058:Pollen / Q000737:chemistry*; D012434:Secale; D012882:Skin Tests
D000485:Allergens