title: Interferon affects cell growth progression by modulating DNA polymerases activity.
abstract: A multiparametric analysis of the effects of human recombinant interferon alpha type A on Daudi cells involving flow cytometry and in vitro analysis of alpha and beta DNA polymerase activities has been performed. Results have disclosed (within 60 min of interferon treatment) a decrease of alpha polymerase driven DNA synthesis persisting to at least 24 h, while beta polymerase was poorly affected. Moreover, after 24 h of interferon treatment, a reduction of BrdUrd incorporation per cell, assessed by flow cytometry, was observed suggesting that DNA synthesis in S phase cells is almost completely abolished. The analysis of the effect of interferon on the distribution of cell cycle phases indicated that the G1/S transition is not inhibited by the treatment. These results support the hypothesis that interferon generates a transient initiating signal which quickly reaches the nucleus and produces a rapid inhibition of alpha polymerase activity, leading finally to the slowing of cell cycle progression.
authors.affiliation: Istituto di Morfologia Umana Normale, Chieti, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cataldi
authors.forename: E
authors.initials: E
authors.lastname: Santavenere
authors.forename: M
authors.initials: M
authors.lastname: Vitale
authors.forename: O
authors.initials: O
authors.lastname: Trubiani
authors.forename: R
authors.initials: R
authors.lastname: Lisio
authors.forename: G
authors.initials: G
authors.lastname: Tulipano
authors.forename: L
authors.initials: L
authors.lastname: Di Domenicantonio
authors.forename: L
authors.initials: L
authors.lastname: Zamai
authors.forename: S
authors.initials: S
authors.lastname: Miscia
mesh_terms: D002453:Cell Cycle / Q000187:drug effects*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000494:pharmacology*; D007700:Kinetics; D014407:Tumor Cells, Cultured
chemical_list: D016898:Interferon-alpha; D004259:DNA-Directed DNA Polymerase