Factors affecting the fast atom bombardment mass spectrometric analysis of proteolytic digests of proteins.
The fast atom bombardment (FAB) mass spectrometric analysis of proteolytic digests of proteins is currently used in protein structural characterization. The major current limitation of this procedure is that not all the peptides generated by enzyme digestion can be observed in the spectra. Previous studies showed that in a mixture the more hydrophilic peptides are suppressed. Several enzymatic digests of 18 different proteins ranging from 10 kDa to 67 kDa in molecular weight were examined using FAB mass spectrometry. It was observed that, even though the hydrophobicity of peptides is a factor in determining their presence or absence in the spectra, the predictions of whether or not a peptide would be detected based on this criterion varies in a wide range of values. Moreover, present results seem to indicate that the presence of particular amino acid side chains within a peptide sequence capable of forming hydrogen bonds with the matrix heavily affects the behaviour of that peptide in the mixture, despite the overall hydrophobicity of the peptide itself.
Pucci
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Servizio di Spettrometria di Massa, CNR-Università di Napoli, Italy.
Sepe
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C
Marino
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D000596:Amino Acids / Q000032:analysis; D006868:Hydrolysis; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000596:Amino Acids; D011506:Proteins