Up-regulation of interleukin (IL)-6 receptor gene expression in vitro and in vivo in IL-6 deprived myeloma cells.
Myeloma cells absolutely require interleukin-6 (IL-6) for growing in vivo in patients with multiple myeloma and exogenous IL-6-dependent myeloma cell lines have been reproducibly obtained. In this study we show a dramatic up-regulation of the IL-6 receptor (gp80 chain) gene expression in myeloma cell lines following the removal of exogenous IL-6. Such a regulation was also known to occur in IL-6-deprived myeloma cells in vivo in three patients who were treated with optimal doses of anti-IL-6 monoclonal antibodies. The direct effect of IL-6 on IL-6 receptor gene expression in myeloma cells was further confirmed by adding IL-6 to an autonomously growing myeloma cell line.
Portier
M
M
INSERM U291, Immunopathologie des Maladies Tumorales et Autoimmunes, Montpellier, France.
Lees
D
D
Caron
E
E
Jourdan
M
M
Boiron
J M
JM
Bataille
R
R
Klein
B
B
D015152:Blotting, Northern; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015850:Interleukin-6 / Q000172:deficiency / Q000378:metabolism*; D009101:Multiple Myeloma / Q000235:genetics* / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism; D019947:Receptors, Interleukin-6; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D015850:Interleukin-6; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6