Common antigenic domains in transferrin-binding protein 2 of Neisseria meningitidis, Neisseria gonorrhoeae, and Haemophilus influenzae type b.
There is now considerable evidence to show that in the Neisseria and Haemophilus species, membrane receptors specific for either transferrin or lactoferrin are involved in the acquisition of iron from these glycoproteins. In Neisseria meningitidis, the transferrin receptor appears to consist of two proteins, one of which (TBP 1) has an M(r) of 95,000 and the other of which (TBP 2) has an M(r) ranging from 68,000 to 85,000, depending on the strain; TBP 2 binds transferrin after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotting, but TBP 1 does not do so. The relative contributions of these two proteins to the binding reaction observed with intact cells and to iron uptake are presently unknown. However, they are being considered as potential components of a group B meningococcal vaccine. Analogous higher- and lower-molecular-weight proteins associated with transferrin binding have been found in N. gonorrhoeae and Haemophilus influenzae. Previous work with polyclonal antibodies raised in mice with whole cells of iron-restricted N. meningitidis showed that the meningococcal TBP 2 exhibits considerable antigenic heterogeneity. Here, we report that antiserum against purified TBP 2 from one strain of N. meningitidis cross-reacts on immunoblotting with the TBP 2 of all meningococcal isolates examined, as well as with the TBP 2 of N. gonorrhoeae. This antiserum also cross-reacted with the TBP 2 of several strains of H. influenzae type b, thus showing the presence of common antigenic domains among these functionally equivalent proteins in different pathogens; no cross-reaction was detected with a purified sample of the human transferrin receptor.
Stevenson
P
P
National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, United Kingdom.
Williams
P
P
Griffiths
E
E
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D002352:Carrier Proteins / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D005260:Female; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D033862:Iron-Binding Proteins; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D009345:Neisseria meningitidis / Q000276:immunology*; D011817:Rabbits; D011990:Receptors, Transferrin / Q000276:immunology; D033882:Transferrin-Binding Protein B; D033863:Transferrin-Binding Proteins
D000942:Antigens, Bacterial; D002352:Carrier Proteins; D007106:Immune Sera; D033862:Iron-Binding Proteins; D011990:Receptors, Transferrin; D033882:Transferrin-Binding Protein B; D033863:Transferrin-Binding Proteins