Five new alleles of plasma Zn-alpha 2-glycoprotein variants phenotyped by isoelectric focusing and immunoblotting in twelve populations.
Human Zn-alpha 2-glycoprotein (ZAG) in plasma samples from twelve populations was tested by immunoblotting after polyacrylamide gel isoelectric focusing. Eleven ZAG phenotypes produced by one common and nine rare alleles, including five new ones (ZAG*6-ZAG*10), were detected. Additionally, an application of separator IEF with N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) was found to be useful for discriminating the rare ZAG 7 band.
Nakayashiki
N
N
Department of Legal Medicine, Iwate Medical University School of Medicine, Morioka, Japan.
Umetsu
K
K
Yuasa
I
I
Suenaga
K
K
Omoto
K
K
Ishida
T
T
Misawa
S
S
Katsura
S
S
D000483:Alleles; D005069:Evaluation Studies as Topic; D005787:Gene Frequency; D014644:Genetic Variation; D006023:Glycoproteins / Q000097:blood / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing / Q000379:methods*; D010641:Phenotype; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein
D006023:Glycoproteins; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein