Release of von Willebrand factor antigen (vWF:Ag) and eicosanoids during acute injury to the isolated rat lung.
It has been suggested that the von Willebrand factor antigen (vWF:Ag) may be a clinical marker for pulmonary endothelial cell injury. An ELISA was developed for the measurement of rat vWF:Ag. Rat lungs were isolated and perfused with a recirculating, blood-free, physiologic salt solution. Circulating levels of vWF:Ag and the eicosanoids thromboxane B2 (TXB2) and prostaglandin 6-keto F1-alpha (6-keto PGF1 alpha) were measured before and after different forms of insult. The addition of phospholipase C (PLC) or hydrogen peroxide (H2O2) to the perfusate caused lung damage as manifested by pulmonary artery pressure increase and pulmonary edema. This was paralleled by significant release of vWF:Ag, TXB2, and 6-keto PGF1 alpha. Increased hydrostatic pressure caused pulmonary edema without vWF:Ag and eicosanoid release. The addition of vasopressin to the perfusate caused vWF:Ag release but no lung injury and no release of eicosanoids. It is concluded that in the rat model, vWF:Ag release is a nonspecific marker for lung injury.
Jones
D K
DK
Cardiovascular Pulmonary Research Laboratory, University of Colorado Health Sciences Center, Denver 80262.
Perry
E M
EM
Grosso
M A
MA
Voelkel
N F
NF
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006874:Hydrostatic Pressure / Q000009:adverse effects; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011654:Pulmonary Edema / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013929:Thromboxane B2 / Q000378:metabolism*; D010738:Type C Phospholipases / Q000494:pharmacology; D014667:Vasopressins / Q000494:pharmacology; D014841:von Willebrand Factor / Q000378:metabolism*
D014841:von Willebrand Factor; D014667:Vasopressins; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D006861:Hydrogen Peroxide; D010738:Type C Phospholipases