Group V allergens in grass pollens: IV. Similarities in amino acid compositions and NH2-terminal sequences of the group V allergens from Lolium perenne, Poa pratensis and Dactylis glomerata.
Monoclonal antibodies (PpV4) raised against Phleum pratense group V allergen were used for immuno-affinity chromatography of cross-reacting group V allergens from related grass species. Fractions enriched in group V allergen were obtained from Lolium perenne, Poa pratense and Dactylis glomerata extracts. The major components in these fractions were found in the Mwr range 25-28 kD. IgE binding to these components was shown using a pool of grass allergic sera, by SDS-PAGE immunoblotting. These fractions were electroblotted from tricine SDS-PAGE gels onto a polyvinylidene-difluoride membrane and selected group V bands were directly cut out and used for amino acid analysis and NH2-terminal sequencing. Both the amino acid compositions and the NH2-terminal sequences obtained for each group V allergen were almost similar to each other and to the sequence and composition of the previously described allergen Phl p V from Phleum pratense. A common trait of the investigated allergens, is the very high contents of alanine (25-32%) and the presence of the modified amino acid, hydroxyproline.
Klysner
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ALK Research, Hørsholm, Denmark.
Welinder
K G
KG
Løwenstein
H
H
Matthiesen
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F
D000485:Allergens / Q000737:chemistry* / Q000145:classification; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D006801:Humans; D007120:Immunochemistry; D051379:Mice; D008969:Molecular Sequence Data; D006109:Poaceae; D011058:Pollen / Q000737:chemistry*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid
D000485:Allergens; D000596:Amino Acids; D000911:Antibodies, Monoclonal