Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations.
Nisin production by Lactococcus lactis subsp. lactis NIZO 22186 was studied in batch fermentation using a complex medium. Nisin production showed primary metabolite kinetics: nisin biosynthesis took place during the active growth phase and completely stopped when cells entered the stationary phase. A stringent correlation could be observed between the expression of the prenisin gene (nisA) and the synthesis of the post-translationally enzymically modified and processed mature nisin peptide. Moreover, it seemed likely that nisin had a growth control function. A physiological link is proposed between sucrose fermentation capacity and nisin production ability. Carbon source regulation appears to be a major control mechanism for nisin production.
De Vuyst
L
L
Laboratory of General and Industrial Microbiology, Faculty of Agricultural Sciences, University of Gent, Belgium.
Vandamme
E J
EJ
D002455:Cell Division / Q000187:drug effects; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D013294:Lactococcus lactis / Q000187:drug effects / Q000378:metabolism*; D009561:Nisin / Q000096:biosynthesis* / Q000494:pharmacology; D011498:Protein Precursors / Q000096:biosynthesis; D011499:Protein Processing, Post-Translational; D013395:Sucrose / Q000378:metabolism
D011498:Protein Precursors; D009561:Nisin; D013395:Sucrose