title: X-ray solution scattering reveals conformational changes upon iron uptake in lactoferrin, serum and ovo-transferrins.
abstract: X-ray solution scattering has been used for studying the structural changes that take place upon uptake and release of iron from serum and chicken ovo-transferrin and human lactoferrin. In the case of chicken ovo-transferrin, data have been obtained for both the intact protein and the isolated N and C-lobes with and without iron. These studies reveal that both lobes undergo a change that is consistent with an opening of the inter-domain cleft when iron is removed from the protein. We suggest that the conformational change of the protein increases the specificity of receptor binding and that the closed configuration of the iron-loaded protein is one, or perhaps the, decisive step in the mechanism for receptor-mediated endocytosis.
authors.affiliation: Molecular Biophysics Group, SERC Daresbury Laboratory, Cheshire, U.K.
authors.forename: J G
authors.initials: JG
authors.lastname: Grossmann
authors.forename: M
authors.initials: M
authors.lastname: Neu
authors.forename: E
authors.initials: E
authors.lastname: Pantos
authors.forename: F J
authors.initials: FJ
authors.lastname: Schwab
authors.forename: R W
authors.initials: RW
authors.lastname: Evans
authors.forename: E
authors.initials: E
authors.lastname: Townes-Andrews
authors.forename: P F
authors.initials: PF
authors.lastname: Lindley
authors.forename: H
authors.initials: H
authors.lastname: Appel
authors.forename: W G
authors.initials: WG
authors.lastname: Thies
authors.forename: S S
authors.initials: SS
authors.lastname: Hasnain
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000737:chemistry; D002645:Chickens; D003198:Computer Simulation; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism*; D007781:Lactoferrin / Q000378:metabolism / Q000648:ultrastructure*; D008958:Models, Molecular; D009038:Motion; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D012542:Scattering, Radiation; D012996:Solutions; D014168:Transferrin / Q000378:metabolism / Q000648:ultrastructure*; D014965:X-Rays
chemical_list: D001059:Apoproteins; D010446:Peptide Fragments; D012996:Solutions; D014168:Transferrin; D007501:Iron; D007781:Lactoferrin