title: Studies on the enzyme immunoassay of bio-active constituents in oriental medicinal drugs. VI. Enzyme immunoassay of ginsenoside Rb1 from Panax ginseng.
abstract: Enzyme immunoassay (EIA) of ginsenoside Rb1 (GRb1), one of the glucosides of protopanaxadiol from Panax ginseng, was explored. A carrier protein (bovine serum albumin (BSA)) was coupled to the C-26 position on the unsaturated side chain of the protopanaxadiol moiety to prepare the immunogen. In order to perform bridge heterologous EIA, a label (beta-D-galactosidase) was introduced at C-26 of the saturated side chain to obtain labeled antigen. Anti-GRb1 antisera were elicited in rabbits by immunization with GRb1-BSA conjugate (9). The double antibody method (with goat anti-rabbit IgG antiserum) was used to separate the bound and free GRb1-beta-Gal. A satisfactory standard curve for EIA of GRb1 was obtained in the range of 0.04-10 ng/tube. In a comparison of the assay results obtained by EIA and HPLC, the linear regression equation and correlation coefficient for the two methods were y (EIA) = 9.18x(HPLC)-0.033 and 0.98, respectively. The anti-GRb1 antiserum cross-reacted with GRb2 (21.8%) and GRc (10.6%), which are also constituents of Panax ginseng.
authors.affiliation: Department of Natural Products Chemistry, Research Institute for Wakan-Yaku Oriental Medicines, Toyama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kanaoka
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: F
authors.initials: F
authors.lastname: Shimada
authors.forename: S
authors.initials: S
authors.lastname: Yano
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D036145:Ginsenosides; D007124:Immunoenzyme Techniques; D009682:Magnetic Resonance Spectroscopy; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal; D011817:Rabbits; D012503:Saponins / Q000032:analysis* / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D036145:Ginsenosides; D012503:Saponins; C048026:ginsenoside Rb2; D012710:Serum Albumin, Bovine