Red blood cell susceptibility to lipid peroxidation, membrane lipid composition, and antioxidant enzymes in continuous ambulatory peritoneal dialysis patients.
OBJECTIVE
To investigate the overall susceptibility of red blood cells (RBC) to lipid peroxidation from patients on continuous ambulatory peritoneal dialysis (CAPD).


METHODS
The following parameters were measured: RBC malondialdehyde (MDA) production after oxidative stress with H2O2, RBC antioxidant enzymes glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), and RBC membrane lipid composition. The levels of plasma vitamin E and serum selenium were also assayed.


METHODS
Eleven patients on continuous ambulatory peritoneal dialysis. Twenty-one healthy blood donors of similar age were used as normal controls.


RESULTS
The MDA formation after H2O2 stimulation was normal in CAPD patients (0.79 +/- 0.1 mumol/gHb versus 0.78 +/- 0.1 in the control group). RBC from CAPD patients also showed a normal SOD activity, a more than adequate vitamin E status, and a peculiar pattern of membrane lipids, with reduced polyunsaturated fatty acids (p less than 0.001) and increased monounsaturated fatty acids (p less than 0.001). Both RBC GSH-Px activity, a selenium-dependent enzyme, and serum selenium levels were significantly lower in CAPD patients, and a significant positive correlation (r = 0.68; p less than 0.02) between the two parameters was found.


CONCLUSIONS
This study found a normal sensitivity to oxidant stress in RBC from a group of CAPD patients, despite an impaired GSH-Px activity. The peculiar lipid pattern of RBC membrane, characterized by reduced PUFA and increased MUFA content, may contribute, in addition to adequate SOD activity and vitamin E status, to normal RBC lipid peroxidation.
Girelli
D
D
Institute of Medical Pathology, University of Verona, Italy.
Lupo
A
A
Trevisan
M T
MT
Olivieri
O
O
Bernich
P
P
Zorzan
P
P
Bassi
A
A
Stanzial
A M
AM
Ferrari
S
S
Corrocher
R
R
D000368:Aged; D000740:Anemia / Q000097:blood / Q000209:etiology*; D004910:Erythrocyte Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D008563:Membrane Lipids / Q000032:analysis*; D010531:Peritoneal Dialysis, Continuous Ambulatory; D013482:Superoxide Dismutase / Q000097:blood*; D014511:Uremia / Q000150:complications / Q000628:therapy
D008563:Membrane Lipids; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase