title: Purification of the 28.5 kDa cytosolic protein involved in the activation of NADPH oxidase from guinea pig neutrophils.
abstract: We tried to purify a new protein component required for the activation of NADPH oxidase from the guinea pig neutrophil cytosolic fraction which did not contain p47phox and p67phox, using HAC-5CP, IEC-QA and Superose 12HR columns. The NADPH oxidase-activating activity was separated into three fractions on IEC-QA anion-exchange HPLC. However, when each of the fractions was purified by Superose 12HR gel filtration, the active fraction eluted at the same position, and was found to contain a common protein with a molecular weight of 28.5 kDa on SDS-PAGE. These results suggest that the 28.5 kDa protein is a novel NADPH oxidase activating protein.
authors.affiliation: Department of Biochemistry, Juntendo University, School of Medicine, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Someya
authors.forename: S
authors.initials: S
authors.lastname: Yomogida
authors.forename: I
authors.initials: I
authors.lastname: Nagaoka
authors.forename: K
authors.initials: K
authors.lastname: Iwabuchi
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D006168:Guinea Pigs; D015151:Immunoblotting; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D019255:NADPH Oxidases; D009504:Neutrophils / Q000201:enzymology*
chemical_list: D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases