title: Sequence similarity between macrolide-resistance determinants and ATP-binding transport proteins.
abstract: The three macrolide-resistance-encoding genes, tlrC from Streptomyces fradiae, srmB from Streptomyces ambofaciens, and carA from Streptomyces thermotolerans, encode proteins that possess significant sequence similarity to ATP-dependent transport proteins. The N-terminal and C-terminal halves of these proteins are very similar to each other and contain highly conserved regions that resemble ATP-binding domains typically present within the superfamily of ATP-dependent transport proteins. These observations suggest that the mechanism by which these genes confer resistance to macrolides is due to export of the antibiotics, a process that is driven by energy derived from ATP hydrolysis.
authors.affiliation: Lilly Research Laboratories, Division of Eli Lilly and Company, Indianapolis, IN 46285.
authors.forename: B
authors.initials: B
authors.lastname: Schoner
authors.forename: M
authors.initials: M
authors.lastname: Geistlich
authors.forename: P
authors.initials: P
authors.lastname: Rosteck
authors.forename: R N
authors.initials: RN
authors.lastname: Rao
authors.forename: E
authors.initials: E
authors.lastname: Seno
authors.forename: P
authors.initials: P
authors.lastname: Reynolds
authors.forename: K
authors.initials: K
authors.lastname: Cox
authors.forename: S
authors.initials: S
authors.lastname: Burgett
authors.forename: C
authors.initials: C
authors.lastname: Hershberger
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry; D004352:Drug Resistance, Microbial / Q000235:genetics; D018942:Macrolides; D008969:Molecular Sequence Data; D011485:Protein Binding; D016415:Sequence Alignment; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D018942:Macrolides; D000255:Adenosine Triphosphate