Indocyanine green disposition in healthy dogs and dogs with mild, moderate, or severe dimethylnitrosamine-induced hepatic disease.
Disposition kinetics of indocyanine green (ICG) were used to evaluate hepatic function in healthy Beagles (group 1; n = 6) and Beagles with progressive hepatic disease induced by oral administration of dimethylnitrosamine, a hepatospecific toxin. Three classes of hepatic disease were defined by histologic features: mild (group 2; n = 5), moderate (group 3; n = 6), and severe (group 4; n = 5). Disposition of ICG was studied 3 weeks following the last dose of toxin. A rapid IV injection of 0.5 mg of ICG/kg was administered and serum samples were obtained at certain intervals during 60-minute periods. Serum ICG was analyzed by use of visible spectrophotometry. Disposition kinetics were determined from serum ICG concentrations vs 15- and 60-minute time curves and compared between one another and among groups. Data based on 60-minute time curves were not significantly different from those based on 15-minute curves. Area under the curve for ICG was greatest in group 3. Clearance of ICG was decreased and mean resident time was increased in groups 3 and 4, compared with those in groups 1 and 2. When disposition data (60 minutes) were normalized for differences in hepatic weight among dogs, group-3 mean resident time was significantly greater than that of group 4. This study supports the diagnostic benefits of using ICG disposition kinetics as a method of evaluating hepatic function in dogs with progressive liver disease.
Boothe
D M
DM
Department of Veterinary Physiology and Pharmacology, Texas Veterinary Medical Center, College of Veterinary Medicine, Texas A&M University, College Station 77843-4466.
Brown
S A
SA
Jenkins
W L
WL
Green
R A
RA
Cullen
J M
JM
Corrier
D E
DE
D000818:Animals; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs / Q000378:metabolism*; D005260:Female; D007208:Indocyanine Green / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007275:Injections, Intravenous / Q000662:veterinary; D008107:Liver Diseases / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D011897:Random Allocation
D007208:Indocyanine Green