Cloning of drebrin A and induction of neurite-like processes in drebrin-transfected cells.
The developmentally-regulated neuron-specific protein, drebrin A, is expressed first at the time of outgrowth and maturation of dendrites, and is localized within dendrites of the adult brain. A cDNA clone of adult rat drebrin A was isolated and sequenced. There is no overall homology with other reported protein sequences except chicken drebrins. We constructed the expression vector MIW-DA containing the drebrin A cDNA. Transfection of nonneuronal cells with MIW-DA induced the formation of highly branched neurite-like cell processes. In these process-bearing transfectants, expressed debrin A is concentrated in submembraneous regions of the cell. Furthermore, actin concentration is higher in these cells than other fibroblasts. These results suggest a possible role of drebrin A in neurite outgrowth.
Shirao
T
T
Laboratory of Neurochemistry, National Institute for Physiological Sciences, Okazaki, Japan.
Kojima
N
N
Obata
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000235:genetics / Q000502:physiology; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D016501:Neurites / Q000378:metabolism* / Q000648:ultrastructure; D009479:Neuropeptides / Q000378:metabolism*; D010957:Plasmids; D051381:Rats; D014162:Transfection
D009479:Neuropeptides; C053386:drebrins; D004247:DNA