Idiotype network responses to murine immunoglobulin G3 anti-carbohydrate antibodies.
Two murine monoclonal antibodies, 8A6 and 8C2, were generated against the carbohydrate moiety of tumor-associated disialoganglioside. Both of the antibodies were of the immunoglobulin G3 (IgG3), K isotype subclass. One of these antibodies (8A6) was used as a network immunogen for the generation of anti-idiotype antibodies. Two AB2 anti-idiotype antibodies were identified. One AB2 (12E5) was subsequently shown to recognize a linear epitope of the 8A6 kappa light chain. The second AB2 (9H8), recognizes a conformational epitope which is dependent on the maintenance of the tertiary structure of the idiotype. Both anti-idiotypes were injected into (syngeneic) mice and (xenogeneic) rabbits to evaluate their effectiveness as "network" antigens in promoting AB3 and anti-carbohydrate AB1' responses. AB3 populations from both syngeneic and xenogeneic hosts were found to be idiotype-specific, yet were unable to produce a measurable subpopulation of anti-tumor (AB1'). These studies suggest that IgG3 isotypes may not be suitable idiotype templates for the mimicry of carbohydrate epitopes.
Anderson
D R
DR
Department of Cellular Immunology, IDEC Pharmaceuticals Corporation, La Jolla, California 92037.
McCoobery
R E
RE
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis*; D015295:Antigens, Tumor-Associated, Carbohydrate; D002241:Carbohydrates / Q000276:immunology*; D005260:Female; D005732:Gangliosides / Q000276:immunology; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D015295:Antigens, Tumor-Associated, Carbohydrate; D002241:Carbohydrates; D005732:Gangliosides; D007074:Immunoglobulin G; C026226:ganglioside, GD3