title: Effects of insulin on diacylglycerol/protein kinase-C signalling and glucose transport in rat skeletal muscles in vivo and in vitro.
abstract: Insulin treatment in vivo provoked rapid dose-related increases in diacylglycerol content and/or translocation of protein kinase-C (PKC) from cytosol to membranes in rat soleus and gastrocnemius muscles. These effects were apparent with 1) insulin doses that provoked submaximal and maximal increases in glucose utilization, and 2) glucose-stimulated endogenous insulin secretion. Insulin-stimulated PKC translocation was evident when PKC was assayed by 1) histone or protamine phosphorylation after PKC purification by Mono Q column chromatography, and 2) immunoblotting for PKC beta and PKC epsilon. Dose-related effects of insulin on PKC translocation were also observed in the rat soleus in vitro, and this was associated with increased phosphorylation of 40- and 80-kilodalton proteins, which were also phosphorylated by phorbol ester treatment. A role for diacylglycerol-PKC signalling in insulin-stimulated glucose transport was suggested by studies of [3H]2-deoxyglucose ([3H]2-DOG) uptake in the rat soleus in vitro in that 1) PKC translocation and 2-DOG uptake were correlated; and 2) stimulatory effects of insulin and phorbol esters on 2-DOG uptake were apparently nonadditive.
authors.affiliation: J.A. Haley Veterans Hospital, Tampa, Florida 33612.
authors.forename: B
authors.initials: B
authors.lastname: Yu
authors.forename: M
authors.initials: M
authors.lastname: Standaert
authors.forename: T
authors.initials: T
authors.lastname: Arnold
authors.forename: H
authors.initials: H
authors.lastname: Hernandez
authors.forename: J
authors.initials: J
authors.lastname: Watson
authors.forename: K
authors.initials: K
authors.lastname: Ways
authors.forename: D R
authors.initials: DR
authors.lastname: Cooper
authors.forename: R V
authors.initials: RV
authors.lastname: Farese
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D002846:Chromatography, Affinity; D003600:Cytosol / Q000201:enzymology; D003847:Deoxyglucose / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D009124:Muscle Proteins / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015398:Signal Transduction / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate
chemical_list: D001786:Blood Glucose; D004075:Diglycerides; D007328:Insulin; D007527:Isoenzymes; D009124:Muscle Proteins; D010710:Phosphates; D010750:Phosphoproteins; D003847:Deoxyglucose; D011493:Protein Kinase C; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate