The effects of transforming growth factor-beta 1 on protein production by mouse embryonic palate mesenchymal cells in the presence or absence of serum.
Mouse embryonic palatal mesenchyme cells were cultured on a variety of substrata (plastic, on a collagen gel or within a collagen gel). On each substratum TGF-beta 1 (1 ng/ml) inhibited cell proliferation. Cells cultured within a collagen gel had the lowest rate of proliferation, but were metabolically the most active in terms of incorporation of [3H]-proline into both collagenous and non-collagenous proteins. TGF-beta 1, in the presence of 2.5% donor calf serum stimulated the production of fibronectin and the major collagen types I, III and V. However, in serum-free medium, TGF-beta 1 induced a large reduction in total collagen production, mainly due to an effect on type I collagen, whilst stimulating production of some non-collagenous proteins. Experiments involving combinations of TGF-beta 1 with other growth factors suggested that the different effects of TGF-beta 1 on collagen production, in the presence and absence of serum, may be due to an interaction with platelet-derived growth factor.
Sharpe
P M
PM
Department of Cell and Structural Biology, University of Manchester, U.K.
Foreman
D M
DM
Carette
M J
MJ
Schor
S L
SL
Ferguson
M W
MW
D000818:Animals; D001769:Blood; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003470:Culture Media; D008648:Mesoderm / Q000378:metabolism*; D051379:Mice; D010159:Palate / Q000196:embryology*; D010969:Plastics; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D003470:Culture Media; D010969:Plastics; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; D003094:Collagen; D011392:Proline