Effect of sodium fluoride on bone density in chickens.
In addition to increasing bone volume, fluoride has been demonstrated to increase ash weight and mineral density. To determine whether newly formed or older bone is most affected by fluoride treatment, bone from chickens receiving fluoridated water was fractionated into lower density (recently formed) and higher density (more mature) specific gravity fractions. Fluoride was administered to the chickens for different lengths of time (4 or 13 weeks) or at varying doses for a 4-week period (0, 4.2, 16.8 mmol/liter drinking water). Fluoride treatment caused a shift in the mineral density profile, showing an increased proportion of mineral distribution in the more mature, higher density fractions. To determine whether this density gradient shift was due to increased maturation rate of bone or decreased resorption and mineralization rates, [3H]proline and 45Ca were injected 5 days and 24 hours prior to sacrifice, respectively. The distributions of both 3H or 45Ca, as percentages of total counts incorporated, were shifted by fluoride treatment into more mature, higher density fractions. Expressing the number of counts as a percent of the bone in each fraction (total hydroxyproline or Ca) revealed an increased incorporation of both 3H and 45Ca into the higher specific gravity fractions 2.0-2.2. These results suggest that fluoride treatment increases bone maturation and the rate of secondary mineralization in the cortical bone. Such changes in the quality of more mature, well-mineralized bone, in humans as well as animals, may have a significant influence on brittleness and strength.
Lundy
M W
MW
Department of Anatomy, Loma Linda University, Pettis Veterans Hospital, California.
Russell
J E
JE
Avery
J
J
Wergedal
J E
JE
Baylink
D J
DJ
D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000378:metabolism; D002132:Calcium Radioisotopes; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D008297:Male; D011392:Proline / Q000378:metabolism; D012969:Sodium Fluoride / Q000494:pharmacology*; D013997:Time Factors; D014316:Tritium
D002132:Calcium Radioisotopes; D014316:Tritium; D012969:Sodium Fluoride; D011392:Proline