title: Effects of androgen on phosphorylation of chromosomal proteins in mouse submandibular gland.
abstract: The effect of androgen on intranuclear distribution of androgen receptor binding sites was studied. The level of the androgen receptor complex in dinucleosomes increased 1 h after androgen treatment and those in the mono- and trinucleosomes increased 3 h after androgen treatment. Investigation by 32P incorporation into the nuclei and active chromatin showed that androgen resulted in an increase in the rate of phosphorylation at 3 h and reduced to the control level at 6 h. These data suggest that association of androgen receptor with the acceptor sites and activities of protein kinase in active chromatin are simultaneously stimulated for activating the transcription by androgen.
authors.affiliation: Department of Biochemistry, Iwate Medical University, School of Dentistry, Morioka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Ota
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007700:Kinetics; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011944:Receptors, Androgen / Q000187:drug effects / Q000378:metabolism; D013363:Submandibular Gland / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D002843:Chromatin; D009687:Nuclear Proteins; D011944:Receptors, Androgen; D013739:Testosterone; D011494:Protein Kinases