title: Identification of Streptomyces coelicolor genes involved in regulation of antibiotic synthesis.
abstract: To define genetic elements that regulate antibiotic synthesis, we screened for mutations that visibly blocked synthesis of Streptomyces coelicolor's two pigmented antibiotics and found mutant strains in which all four antibiotics were blocked. The responsible mutations defined two loci, absA and absB. Two additional approaches to defining genes have been taken: isolation of cloned genes with a dominant negative effect on antibiotic synthesis and isolation of genes which, in multicopy, can compensate for specific mutational blocks. These genes apparently function in a global regulatory pathway (or network) for control of antibiotic synthesis.
authors.affiliation: Department of Microbiology, Michigan State University, East Lansing 48824-1101.
authors.forename: W
authors.initials: W
authors.lastname: Champness
authors.forename: P
authors.initials: P
authors.lastname: Riggle
authors.forename: T
authors.initials: T
authors.lastname: Adamidis
authors.forename: P
authors.initials: P
authors.lastname: Vandervere
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002874:Chromosome Mapping; D015964:Gene Expression Regulation, Bacterial; D005799:Genes, Dominant; D009154:Mutation; D016366:Open Reading Frames; D010860:Pigments, Biological; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D010860:Pigments, Biological