Eight base changes are sufficient to convert a leucine-inserting tRNA into a serine-inserting tRNA.
Each aminoacyl-tRNA synthetase must functionally distinguish its cognate tRNAs from all others. We have determined the minimum number of changes required to transform a leucine amber suppressor tRNA to serine identity. Eight changes are required. These are located in the acceptor stem and in the D stem.
Normanly
J
J
Division of Biology, California Institute of Technology, Pasadena 91125.
Ollick
T
T
Abelson
J
J
D001482:Base Composition; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D005813:Genes, Synthetic; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D012356:RNA, Transfer, Leu / Q000235:genetics*; D012362:RNA, Transfer, Ser / Q000235:genetics*; D013489:Suppression, Genetic
D009838:Oligodeoxyribonucleotides; D012356:RNA, Transfer, Leu; D012362:RNA, Transfer, Ser