title: Prenatal microbiological risk factors associated with preterm birth.
abstract: OBJECTIVE
  To study the vaginal flora of pregnant women at 22-28 weeks gestation to determine whether the presence of specific micro-organisms is significantly associated with preterm birth and prelabour rupture of the membranes.
  
  
  METHODS
  A comprehensive descriptive prospective study of the vaginal micro-flora of women between 22-28 weeks gestation comparing those who gave birth preterm (less than 37 weeks) with those who gave birth at term. Microbiological assessment included cultures for aerobic and anaerobic bacteria, yeasts, genital mycoplasmas and Trichomonas vaginalis. Multiple logistic regression analysis was used to account for confounding obstetric and demographic variables.
  
  
  METHODS
  The Queen Victoria Hospital, Adelaide, South Australia.
  
  
  METHODS
  135 women who gave birth preterm compared to 651 women who gave birth at term.
  
  
  METHODS
  Preterm birth and preterm prelabour rupture of membranes (PROM) RESULTS: The prevalence of Gardnerella vaginalis between 22-28 weeks was significantly higher in women who gave birth preterm compared to women who gave birth at term (23% vs 15%; multiple logistic regression odds ratio (OR) 1.8, 95% confidence intervals (CI) 1.01-3.2, P less than 0.05. Ureaplasma urealyticum was also found in a higher proportion of women who gave birth preterm (49% vs 32% OR 1.7, 95% CI 1.1-2.6, P less than 0.0005). Preterm PROM occurred in 42% of whom 60% were carriers of U. urealyticum between 22-28 weeks, compared with 32% in the term group (OR 3.2, CI 1.7-6.1, P less than 0.0005). When women who received antibiotics between the midtrimester swab and labour were excluded, G. vaginalis was also significantly associated with preterm PROM (OR 2.7, CI 1.1-6.5, P less than 0.05). The presence of vaginal enteropharyngeal bacteria (E. coli, Klebsiella spp., Haemophilus spp., Staph. aureus) in the midtrimester was not predictive of preterm birth, but when these organisms were found in labour, they appeared to have been acquired later in the pregnancy.
  
  
  CONCLUSIONS
  Women carrying G. vaginalis or U. urealyticum during the midtrimester had nearly twice the risk of preterm birth, while women positive for U. urealyticum had more than a threefold risk of preterm PROM.
authors.affiliation: Adelaide Medical Centre for Women and Children, Queen Victoria Hospital, Australia.
authors.forename: H M
authors.initials: HM
authors.lastname: McDonald
authors.forename: J A
authors.initials: JA
authors.lastname: O'Loughlin
authors.forename: P
authors.initials: P
authors.lastname: Jolley
authors.forename: R
authors.initials: R
authors.lastname: Vigneswaran
authors.forename: P J
authors.initials: PJ
authors.lastname: McDonald
mesh_terms: D000328:Adult; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000382:microbiology*; D006194:Gardnerella vaginalis / Q000302:isolation & purification*; D006801:Humans; D007752:Obstetric Labor, Premature / Q000382:microbiology*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D012307:Risk Factors; D016869:Ureaplasma Infections / Q000150:complications; D016990:Ureaplasma urealyticum / Q000302:isolation & purification*; D014621:Vagina / Q000382:microbiology*