Characterization and autoradiographic localization of histamine H1 receptors in human nasal turbinates.
To examine the localization of histamine H1 receptors (H1R) in human nasal mucosa, the autoradiographic distribution of H1R was studied in human nasal inferior turbinates. Cryostat sections were incubated with various concentration of [3H]pyrilamine in saturation-binding studies and with 1 nmol/L of [3H]pyrilamine for autoradiography. Nonspecific binding was determined by adding 2 mumol/L of pyrilamine. Scatchard analysis demonstrated high-affinity binding sites with a maximum binding capacity of H1R of 193 +/- 46 fmol/mg of protein, and dissociation constant was 0.6 +/- 0.1 nmol/L. Autoradiograms indicated H1R exist exclusively on the endothelium of vessels. No specific labeling could be observed in the submucosal glands or epithelium. These results extend and support our previous finding that histamine directly causes vascular permeability through H1R and stimulates nasal glandular secretion indirectly through reflexes.
Okayama
M
M
Allergic Disease Section, National Institute of Allergy and Infectious Diseases, Bethesda, Md. 20891.
Baraniuk
J N
JN
Hausfeld
J N
JN
Merida
M
M
Kaliner
M A
MA
D001345:Autoradiography; D001665:Binding Sites; D004730:Endothelium, Vascular / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D009297:Nasal Mucosa / Q000737:chemistry; D011738:Pyrilamine; D011869:Radioligand Assay / Q000379:methods; D011969:Receptors, Histamine H1 / Q000032:analysis*; D014420:Turbinates / Q000737:chemistry*
D011969:Receptors, Histamine H1; D011738:Pyrilamine