[A comparative study of the effect of pyrazole on the activity of enzymes in the alcohol/polyol dehydrogenase family].
A comparative study of the effect of pyrazol, an inhibitor of the coenzyme-binding site of alcohol dehydrogenases, on the activity of enzymes of the alcohol/polyol dehydrogenase group has been carried out. Commercial preparations of alcohol dehydrogenases from the cytoplasm of horse liver cells and yeast cells, as well as the enzyme from the cytoplasm of Trichosporon pullulans cells was completely inhibited by 1 mM pyrazol, while alcohol dehydrogenases from Candida utilis and Saccharomyces carlsbergensis were inhibited only by 25% and the enzymes from Saccharomyces cerevisiae and Torulopsis candida by 30 and 38%, respectively. The inhibition degree of alcohol dehydrogenases from the cytoplasm of liver cells of various mammals (bull, calf, rat, gopher) and birds (hen, pheasant, duck) varied from 12 to 42% in the presence of 1 mM pyrazol. The activity of sorbitol dehydrogenase from the liver cytoplasm of these mammals and birds changed neither in the presence of 1 mM pyrazol, nor in the case of a 15-fold increase of the inhibitor concentration. Possible structural differences in the coenzyme-binding site of the active center of the enzymes under study are discussed.
Sudovtsov
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D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000449:Aldehyde Reductase; D000818:Animals; D002175:Candida / Q000201:enzymology; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D011720:Pyrazoles / Q000494:pharmacology*; D012440:Saccharomyces / Q000201:enzymology; D013401:Sugar Alcohol Dehydrogenases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014250:Trichosporon / Q000201:enzymology
D011720:Pyrazoles; C031280:pyrazole; D013401:Sugar Alcohol Dehydrogenases; D000426:Alcohol Dehydrogenase; D007064:L-Iditol 2-Dehydrogenase; D000449:Aldehyde Reductase; C023142:polyol dehydrogenase (NADP)
Sravnitel'noe issledovanie vliianiia pirazola na aktivnost' fermentov semeĭstva alkogol'/polioldegidrogenaz.