Protein A potentiates lymphokine-activated killer cell induction in normal and melanoma patient lymphocytes.
The effects of purified protein A from Staphylococcus aureus Cowan I stain on induction of lymphokine (IL-2) activated killer (LAK) activity were studied in normal as well as melanoma patient's lymphocyte. The coculture of peripheral blood mononuclear cells (PBMC) with various doses of protein A (0.001, 0.01 and 0.1 microgram/ml) and IL-2 (100 U/ml) for 4 days produced synergistic effect on the LAK cells mediated cytotoxicity. The potentiation of cytotoxicity and lytic ability of LAK cells against NK sensitive (K-562) and NK-resistant (M14) tumor cells were observed. Further there was potentiation of DNA synthesis in PBMC after 4 days culture. Similar results were found when PBMC from melanoma patients were cultured with PA and IL-2. The potentiation of LAK cell induction associated with its cytotoxic and lytic potential by low doses of IL-2/PA regiment may be helpful in the development of LAK immunotherapy of the cancer patients.
Singh
K P
KP
Preventive Toxicology Division, Industrial Toxicology Research Centre, Lucknow, India.
Shau
H
H
Gupta
R K
RK
Kopald
K
K
Ray
P K
PK
D004357:Drug Synergism; D006801:Humans; D016219:Immunotherapy, Adoptive; D007376:Interleukin-2 / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects* / Q000276:immunology; D008545:Melanoma / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D013205:Staphylococcal Protein A
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D013205:Staphylococcal Protein A