title: Kluyveromyces contains a functional ABF1-homologue.
abstract: ABF1 is a multifunctional protein present in Saccharomyces cerevisiae, involved in transcription-activation and -repression as well as in DNA-replication. Several lines of evidence indicate the occurrence in the related species Kluyveromyces lactis of a protein having similar properties to those of ABF1 in S. cerevisiae. In order to identify conserved functional domains in ABF1, we have cloned and sequenced the gene encoding the ABF1-homologue from K. lactis. KIABF1 is much smaller than ScABF1 (54.6 vs. 81.7 kD). It exhibits extensive homology with its S. cerevisiae counterpart in the N-terminal region. The C-terminal domain however, is divergent, with the striking exception of a stretch of 20 amino acids, which is virtually identical in the two proteins. KIABF1 can substitute ABF1 in S. cerevisiae, emphasizing the conservation of the multiple functions of this protein.
authors.affiliation: Department of Biochemistry and Molecular Biology, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: P M
authors.initials: PM
authors.lastname: Gonçalves
authors.forename: K
authors.initials: K
authors.lastname: Maurer
authors.forename: W H
authors.initials: WH
authors.lastname: Mager
authors.forename: R J
authors.initials: RJ
authors.lastname: Planta
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D007716:Kluyveromyces / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C061886:ABF1 protein, S cerevisiae; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors