Myocardial dystrophin immunolocalization at sarcolemma and transverse tubules.
Using monoclonal antibodies against two different regions of the helical rod part of dystrophin, we have localized dystrophin on both plasma membrane and transverse tubules in cardiac muscle of man and several animal species. The staining persisted after experimental ischaemia, and was observed in long-standing heart disease. No immunostaining was seen at the intercalated discs. In skeletal muscle the same two antibodies stained only the plasma membrane.
Yarom
R
R
Hadassah Medical School, Jerusalem, Israel.
Morris
G E
GE
Froede
R
R
Schaper
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D002311:Cardiomyopathy, Dilated / Q000378:metabolism; D003327:Coronary Disease; D016189:Dystrophin / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009206:Myocardium / Q000737:chemistry* / Q000648:ultrastructure; D011817:Rabbits; D012508:Sarcolemma / Q000737:chemistry*; D013552:Swine; D013556:Swine, Miniature
D000911:Antibodies, Monoclonal; D016189:Dystrophin