Immunoreactivity of a mammalian liver component with leprosy sera.
Sera from 77 leprosy patients in various stages of infection--tuberculoid (TT), lepromatous (LL), borderline tuberculoid and borderline lepromatous--15 contacts and 21 normal healthy individuals, were assayed in an indirect enzyme-linked immunosorbent assay and dot enzyme immunoassay using ethanol-soluble and thermostable extract of liver as the antigen. The highest incidences of reaction were found in untreated LL patients (100%) and in TT patients (91%), while the sera from borderline patients showed a comparatively lower incidence (43%). Some of the sera from contacts of leprosy patients (6/15) also showed high reactivity. Assays using lecithin as an antigen did not exhibit any reaction.
Deshpande
R G
RG
Department of Microbiology and Immunology, Morehouse School of Medicine, Atlanta, Ga. 30310.
Khan
M B
MB
Navalkar
R G
RG
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D005779:Immunodiffusion; D007918:Leprosy / Q000276:immunology*; D008099:Liver / Q000276:immunology*; D010713:Phosphatidylcholines / Q000276:immunology; D011817:Rabbits; D051381:Rats
D010713:Phosphatidylcholines