Research note: antioxidant activity of Japanese quail liver cytosol in the absence and presence of reduced glutathione.
The antioxidant activity of Japanese quail liver cytosol was assessed in the presence of .1, 1.0, or 10.0 mM reduced glutathione (GSH) using an in vitro assay system that utilized quail liver microsomes as the lipid substrate. The formation of malonaldehyde (MA), an indicator of lipid peroxidation, was reduced (P less than .01) by 1.0 and 10.0 mM GSH, even in the absence of cytosolic protein. Cytosolic protein at a level of 1 mg/mL of assay medium did not further reduce MA formation at any of the GSH concentrations tested. Increasing the cytosolic protein concentration to 6 mg/mL decreased (P less than .01) MA formation, even in the absence of added GSH.
Spurlock
M E
ME
Department of Animal Sciences, University of Missouri, Columbia 65211.
Savage
J E
JE
D000818:Animals; D003370:Coturnix / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*
D008315:Malondialdehyde; D005978:Glutathione; D010100:Oxygen