title: Dynamic aspects of ovarian superoxide dismutase isozymes during the ovulatory process in the rat.
abstract: To investigate the role of superoxide dismutase (SOD) in the ovulatory process, SOD isozymes and their mRNAs were determined in the ovary of 22-day-old rats. After treatment with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG), ovarian activity of Mn-SOD decreased markedly while Cu/Zn-SOD remained unchanged. However, the ovarian level of mRNA for Mn-SOD markedly increased after hCG-treatment while that for Cu/Zn-SOD decreased only slightly. Ovulation was inhibited by intravenous injection of a long-acting SOD. These results suggested that superoxide radicals in the ovary might play a critical role in the mechanism for hCG-induced ovulation.
authors.affiliation: Department of Medical Biology, Kochi Medical School, Japan.
authors.forename: E F
authors.initials: EF
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Kobuchi
authors.forename: K
authors.initials: K
authors.lastname: Edashige
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Yoshioka
authors.forename: K
authors.initials: K
authors.lastname: Utsumi
authors.forename: M
authors.initials: M
authors.lastname: Inoue
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D005260:Female; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D010053:Ovary / Q000201:enzymology* / Q000502:physiology; D010060:Ovulation / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D013482:Superoxide Dismutase