Induction of DNA-repair synthesis (UDS) in rat pleural mesothelial cells by urine of subjects exposed to genotoxic agents.
Unscheduled DNA synthesis was determined in confluent rat pleural mesothelial cells arrested in G0/G1 with hydroxyurea by the measurement of [3H]thymidine incorporation into DNA. Cells were treated with concentrated urine or serum from subjects exposed to certain genotoxic agents, i.e. eight cancer patients treated with radiotherapy and/or chemotherapy (r/c cancer patients) and six chromium workers. Two additional groups consisted of six nonoccupationally exposed healthy smokers and five control volunteers who were nonsmokers and nonexposed. [3H]thymidine incorporated into DNA of all samples was measured by liquid scintillation counting and of urine samples from r/c cancer patients by autoradiography. Compared to the level observed in untreated cells, a statistically significant increased [3H]thymidine incorporation was found in cells treated with urine from 7 of 8 r/c cancer patients and from 5 of 6 chromium workers. In contrast, urine from control volunteers had no effect on the unscheduled DNA synthesis response and urine from only one smoker significantly enhanced [3H]thymidine incorporation into DNA. No clear-cut difference between groups was obtained with serum. These results suggest that urine could be useful to monitor subjects exposed to genotoxic agents.
Pilliere
F
F
Chu Henri Mondor, Créteil, France.
Levy
F
F
Renier
A
A
Brochard
P
P
Jaurand
M C
MC
D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D002857:Chromium / Q000652:urine; D004260:DNA Repair; D004261:DNA Replication; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D006918:Hydroxyurea; D008875:Middle Aged; D009152:Mutagenicity Tests; D009369:Neoplasms / Q000628:therapy / Q000652:urine; D016273:Occupational Exposure; D010994:Pleura / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D012907:Smoking / Q000652:urine; D013861:Thiocyanates / Q000652:urine; D013936:Thymidine / Q000378:metabolism; D014556:Urine
D013861:Thiocyanates; D002857:Chromium; D013936:Thymidine; D006918:Hydroxyurea