An interpretation of cell separation mechanism on polyamine graft copolymers.
Interaction between polyamide microcapsules having different balances of negative and positive charges on their surface, that is, different isoelectric points and copolymers with different numbers of polyamine macromer of a definite chain length grafted on poly(2-hydroxyethyl methacrylate) backbone was studied by measuring the amount adhered of the microcapsules onto the surface of copolymer-coated glass beads at different pH values. Maximum microcapsule adhesion was observed for a proper combination of microcapsule and copolymer to suggest that separation of a specified cell population from others on the surface of copolymer-coated glass beads can be explained in terms of preferential adhesion through electrostatic interaction of the specified cells with the copolymer.
Yoshida
Y
Y
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Shinjuku-ku, Japan.
Muramatsu
N
N
Kataoka
K
K
Ohshima
H
H
Kondo
T
T
D002469:Cell Separation / Q000295:instrumentation*; D005898:Glass; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008863:Microspheres; D009994:Osmolar Concentration; D011073:Polyamines; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives*; D013499:Surface Properties
D011073:Polyamines; C056971:poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; D011102:Polyhydroxyethyl Methacrylate