title: The Escherichia coli K-12 cyn operon is positively regulated by a member of the lysR family.
abstract: A regulatory gene, cynR, was found to be located next to the cyn operon but transcribed in the opposite direction. cynR encodes a positive regulatory protein that controls the cyn operon as well as its own synthesis. Positive regulation of the cyn operon requires cyanate and the cynR protein, but the negative autoregulation of the cynR gene appears to be independent of cyanate. The predicted amino acid sequence of the cynR protein derived from the DNA sequence was found to have significant homology to the predicted amino acid sequence of the lysR family of regulatory proteins.
authors.affiliation: Department of Biochemistry, University of Minnesota, St. Paul 55108.
authors.forename: Y C
authors.initials: YC
authors.lastname: Sung
authors.forename: J A
authors.initials: JA
authors.lastname: Fuchs
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003485:Cyanates / Q000494:pharmacology; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009876:Operon / Q000187:drug effects / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D015533:Transcriptional Activation
chemical_list: D001426:Bacterial Proteins; D003485:Cyanates; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C074784:cynR protein, E coli; C073470:LysR protein, Bacteria