Transport of 3,5,3'-triiodothyronine into the perfused rat liver and subsequent metabolism are inhibited by fasting.
The effects of 48-h fasting on transport of T3 and subsequent metabolism in the isolated perfused rat liver were investigated. Tracer T3 disappearance curves from the recirculating medium consisted of a fast component (FC) and a slow component (SC). Using a two-compartment model, both transport [expressed as the fractional transport rate constant from medium to liver (k21)] and disposal of T3 were calculated. After fasting, k21, total metabolism, and metabolism corrected for differences in mass transfer were diminished, pointing to both decreased transport and metabolism, presumably caused by depletion of liver ATP. Concerning transport, it was shown that only transport into the intracellular liver compartment and not transport to the extracellular liver compartment was decreased after fasting. As for metabolism, T3 glucuronidation was diminished; T3 sulfation and subsequent deiodination were not affected. All mentioned decreased parameters normalized after the addition of a combination of insulin, cortisol, and/or glucose to the medium, possibly by (partially) restoration of cellular energy stores.
De Jong
M
M
Department of Internal Medicine III, Medical Faculty, Erasmus University, Rotterdam, The Netherlands.
Docter
R
R
Van Der Hoek
H J
HJ
Vos
R A
RA
Krenning
E P
EP
Hennemann
G
G
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D005215:Fasting / Q000502:physiology*; D005502:Food; D005947:Glucose / Q000494:pharmacology; D005965:Glucuronates / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000378:metabolism; D014284:Triiodothyronine / Q000378:metabolism*
D005965:Glucuronates; D007328:Insulin; D013431:Sulfates; D014284:Triiodothyronine; D000255:Adenosine Triphosphate; D005947:Glucose; D006854:Hydrocortisone