Transfer of N-acetylglucosamine to nuclear endogenous acceptors of rat hepatocytes.
1. Nuclei were prepared from rat hepatocytes. A biochemical analysis of marker enzymes showed that the nuclei are not contaminated by other subcellular fractions, especially endoplasmic reticulum. 2. The transfer of [14C]N-acetylglucosamine to endogenous acceptors were studied comparatively in the nuclei and in the other subcellular fractions of rat hepatocytes. 3. In this report we describe the presence of the transfer of N-acetylglucosamine within the nucleus of rat hepatocytes. We found 21% of this transfer in the nucleus fraction with an enrichment of 26 in comparison to homogenate.
Bellemin-Magninot
P
P
Laboratoire de Biochimie des Membranes (LBTM-CNRS UMR 24), Université Claude Bernard Lyon I, Villeurbanne, France.
Azzar
G
G
Létoublon
R
R
Got
R
R
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000378:metabolism; D034781:Receptors, N-Acetylglucosamine; D013347:Subcellular Fractions / Q000378:metabolism
D011971:Receptors, Immunologic; D034781:Receptors, N-Acetylglucosamine; D000117:Acetylglucosamine