title: Fasting induced alterations in mitochondrial palmitoyl-CoA metabolism may inhibit adipocyte pyruvate dehydrogenase activity.
abstract: 1. Adipocytes from fed and fasted (24 hr) groups of rats were fractionated into mitochondria, microsomes and plasma membranes. 2. Fasting significantly decreased the mitochondrial activity of palmitoyl-CoA synthetase, palmitoyl-CoA hydrolase, beta-oxidation and pyruvate dehydrogenase. 3. Fasting elevated intramitochondrial long-chain acyl-CoA. 4. Pyruvate dehydrogenase was inhibited 50% by addition of 30 microM palmitoyl-CoA. 5. Fasting-induced changes in palmitoyl-CoA metabolism may modulate pyruvate dehydrogenase activity in adipocyte mitochondria.
authors.affiliation: Department of Chemistry, Oakland University, Rochester, MI 48309-4401.
authors.forename: K H
authors.initials: KH
authors.lastname: Moore
authors.forename: D M
authors.initials: DM
authors.lastname: Dandurand
authors.forename: F L
authors.initials: FL
authors.lastname: Kiechle
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D005215:Fasting / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000378:metabolism*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011768:Pyruvate Dehydrogenase Complex; D010171:Palmitoyl Coenzyme A; D008274:Magnesium