Lactate transport during differentiation of skeletal muscle cells: evidence for a specific carrier in L6 myotubes.
Cellular uptake of lactate involves a carrier in numerous types of mammalian cells. In cultured rat L6 myoblasts, lactate diffuses freely. We show that during myogenesis a carrier appears at the myotube stage. The degree of cellular differentiation was determined by microscopic observation and by measurement of a marker of biochemical differentiation: the increase in specific activity of creatine kinases.
el Abida
K
K
Laboratoire de physiologie des adaptations, CHU Cochin, Paris, France.
Duvallet
A
A
Thieulart
L
L
Rieu
M
M
Beaudry
M
M
D000818:Animals; D001693:Biological Transport, Active; D002454:Cell Differentiation; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D007700:Kinetics; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D009132:Muscles / Q000166:cytology / Q000378:metabolism*
D007773:Lactates; D019344:Lactic Acid; D003402:Creatine Kinase