A new alpha-helical coiled coil protein encoded by the Salmonella typhimurium virulence plasmid.
A new protein of Salmonella typhimurium was identified and characterized. The gene (tlpA) encoding this protein (TlpA) was isolated from the large virulence-associated plasmid of S. typhimurium and sequenced in order to predict the primary structure of TlpA. tlpA encodes a 371-amino acid soluble protein with a calculated M(r) of 41600 and pI of 4.63. Secondary structure predictions and sequence statistics of TlpA indicated a predominant alpha-helical configuration and presence of heptapeptide repeat motifs characteristic of coiled coil proteins. Purified TlpA was shown to have biochemical properties similar to those of coiled coil proteins, including adoption of an alpha-helical configuration and a tendency to form homodimers. Furthermore, TlpA possessed heat resistance, evidence for a chain register and altered mobility in urea/sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels which are characteristics of tropomyosins. TlpA shows 32% overall sequence similarity with rat cardiac myosin and 36% similarity with horse platelet beta-tropomyosin over 226 residues, whereas selected regions possessed significant sequence identities with myosins, tropomyosins, and alpha-helical surface proteins of Streptococcus pyogenes. Our results indicate that TlpA represents a new member of prokaryotic coiled coil proteins.
Koski
P
P
Department of Biochemistry, University of Helsinki, Finland.
Saarilahti
H
H
Sukupolvi
S
S
Taira
S
S
Riikonen
P
P
Osterlund
K
K
Hurme
R
R
Rhen
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002942:Circular Dichroism; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010957:Plasmids; D011487:Protein Conformation; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000378:metabolism* / Q000472:pathogenicity; D012689:Sequence Homology, Nucleic Acid; D013056:Spectrophotometry, Ultraviolet; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C075138:TlpA protein, Salmonella