Numerical chromosome anomalies after fertilization of freeze-thawed mouse oocytes.
The chromosome complement of first cleavage stage mouse embryos was analyzed to investigate the effect of slow freezing-fast thawing cryopreservation on chromosome numbers by comparing these numbers with those found fresh after fertilization of control oocytes. Fewer frozen-thawed (34.1%) than control oocytes (75.0%) cleaved to the 2-cell stage after in vitro fertilization. The incidence of hyperploidy was significantly increased by freezing (4.5% vs. 0% in controls). Polyploidy was not significantly affected (17.0% for freeze-thaw embryos vs. 26.2% for controls).
Sterzik
K
K
Department of Gynecology and Obstetrics, University of Ulm, FRG.
Rosenbusch
B
B
Grab
D
D
Wahl
A
A
Beier
H M
HM
Lauritzen
C
C
D000782:Aneuploidy; D000818:Animals; D002869:Chromosome Aberrations / Q000235:genetics*; D025063:Chromosome Disorders; D015925:Cryopreservation; D004622:Embryo, Mammalian / Q000648:ultrastructure; D005260:Female; D005307:Fertilization in Vitro; D051379:Mice; D008807:Mice, Inbred BALB C; D009865:Oocytes