A novel isoform of rat hepatocyte nuclear factor 4 (HNF-4).
Based on the published nucleotide sequence for rat hepatocyte nuclear factor 4 (HNF-4; Sladek, F.M., Zhong, W., Lai, E. and Darnell, J.E., Jr. (1990) Genes Dev. 4, 2353-2365), we have cloned a cDNA by means of polymerase chain reaction amplification of reverse-transcribed RNA (RT-PCR). Our clone contained an extra segment of 30 bp, which was not found in the previously reported clone, in the coding region near the C-terminus. Further RT-PCR analysis demonstrated that both isoforms of HNF-4 mRNA, i.e., with or without the 30 nucleotide segment, occur in rat liver and kidney, presumably by differential splicing.
Hata
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S
Faculty of Science, Himeji Institute of Technology, Hyogo, Japan.
Tsukamoto
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Osumi
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T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D051557:Hepatocyte Nuclear Factor 4; D008099:Liver / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D010750:Phosphoproteins; D016133:Polymerase Chain Reaction; D051381:Rats; D011919:Rats, Inbred Strains; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D051557:Hepatocyte Nuclear Factor 4; D010750:Phosphoproteins; D014157:Transcription Factors; D004247:DNA