Effects of growth medium, electrical stimulation and paralysis on various enzyme activities in cultured rat muscle cells. Comparison with activities in rat muscles in vivo.
1. Replacement of fetal calf serum and chicken embryo extract by Ultroser G and rat brain extract during the proliferation phase resulted in a higher maturation grade of cultured rat muscle cells after 7 days of differentiation, on base of the percentage of the muscle specific isoenzyme of creatine kinase (CK-MM). 2. Furthermore, the activities of creatine kinase, citrate synthase, cytochrome c oxidase and hexokinase were significantly higher. 3. Compared to the enzyme activities in m. quadriceps of 10 day-old rat and m. quadriceps, m. soleus and m. extensor digitorum longus of young adult rats, the metabolic capacity of cultured myotubes most closely resembles that of the first muscle. 4. Paralysis with tetrodotoxin caused a slight decrease of the creatine kinase activity and the percentage of CK-MM of cultured myotubes and an increase of the activities of hexokinase, phosphorylase and AMP deaminase. 5. Electrical stimulation performed at different frequencies and time periods had no effect on the enzyme activities of cultured rat muscle cells. 6. Only the AMP deaminase activity was decreased after intense electrical stimulation.
Jacobs
A E
AE
Department of Biochemistry, University of Nijmegen, The Netherlands.
Benders
A A
AA
Oosterhof
A
A
Veerkamp
J H
JH
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004558:Electric Stimulation; D009119:Muscle Contraction / Q000502:physiology*; D009132:Muscles / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D003470:Culture Media