Structure of a heteroglycan isolated from the fungus Omphalia lapidescens.
The major, water-soluble polysaccharide isolated from the sclerotia of Omphalia lapidescens, and purified by chromatography on DEAE-Sephadex A-50 and Toyopearl HW-65F, is a heteroglycan (OL-3), [alpha]D20 - 50 degrees (c 1, water), which is composed of D-glucose, 2-acetamido-2-deoxy-D-glucose, and D-glucuronic acid in the molar ratios 1.21:1.00:1.38. Methylation analysis, partial acid hydrolysis, and f.a.b.-m.s. of the hydrolysates indicated OL-3 to have a highly branched structure involving (1----3)-, (1----4)-, (1----6)-, and (1----3,6)-linked, and non-reducing terminal D-glucopyranosyl residues, and also to contain 2 acetamido-2-deoxy-beta-D-glucopyranosyl-(1----4)-beta-D- glucopyranosyluronic acid and beta-D-glucopyranosyl-(1----4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl -(1----4)-beta-D-glucopyranosyluronic acid segments.
Saito
K
K
Tokyo Metropolitan Research Laboratory of Public Health, Japan.
Nishijima
M
M
Miyazaki
T
T
D000363:Agaricales / Q000737:chemistry*; D000871:Anthelmintics / Q000737:chemistry*; D002240:Carbohydrate Sequence; D010450:Endopeptidases / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis
D000871:Anthelmintics; D006025:Glycosaminoglycans; D009005:Monosaccharides; D010450:Endopeptidases