title: Destabilization of whole skin lipid bio-liposomes induced by skin penetration enhancers and FT-IR/ATR (Fourier transform infrared/attenuated total reflection) analysis of stratum corneum lipids.
abstract: Whole skin lipid bio-liposomes (skin bio-liposomes), in size ranging from 2 to 8 microns, were prepared by a reverse phase evaporation technique using rat full thickness skin. Leakage of an encapsulated fluorescence probe, ANTS (delta-amino-1,3,6-naphthalene-trisulfonate), was measured by adding transdermal penetration enhancers (penetrants) into the medium where the skin bio-liposomes were present. Oleylamine induced a fast release of ANTS from the liposomes compared to lauryl-amine which showed a weak action. With these penetrants, the degree of ANTS release from the prepared bio-liposomes was found to correlate well with the results of frequency changes in the CH-asymmetric stretching band near 2920 cm-1 in the rat stratum corneum. The penetrant which caused relatively strong leakage of ANTS induced the significantly large shift of the peak toward the higher wave-numbers due to the perturbation in the structure of lipids of the stratum corneum. The skin bio-liposomes prepared from the rat full thickness skin could be useful in evaluating the penetrants.
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: H
authors.initials: H
authors.lastname: Yasukawa
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaoka
authors.forename: Y
authors.initials: Y
authors.lastname: Morimoto
authors.forename: S
authors.initials: S
authors.lastname: Nakao
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumori
authors.forename: T
authors.initials: T
authors.lastname: Fukuda
mesh_terms: D000588:Amines / Q000494:pharmacology*; D000818:Animals; D005583:Fourier Analysis; D008081:Liposomes / Q000378:metabolism*; D008297:Male; D009281:Naphthalenes / Q000493:pharmacokinetics; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012869:Skin Absorption / Q000187:drug effects*
chemical_list: D000588:Amines; D008081:Liposomes; D009281:Naphthalenes; D009829:Oleic Acids; D019301:Oleic Acid; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid; C003935:dodecylamine; C008703:oleylamine