title: Rapid and simple isolation of DNA from agarose gels.
abstract: We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 and 20 kb in size is achieved by concentrating the DNA flushed from the gel in a DNA-binding column.
authors.affiliation: Institut für Mikrobiologie, J. W. Goethe-Universität, Frankfurt/Main, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Grey
authors.forename: M
authors.initials: M
authors.lastname: Brendel
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D005782:Gels; D012685:Sepharose
chemical_list: D005782:Gels; D004247:DNA; D012685:Sepharose