[Biotin-avidin-ELISA for cytotoxin purified from cobra venom].
A technique combining the biotin-avidin amplifying system with sandwich enzyme-linked immunosorbent assay (B-A-ELISA) was developed for the microassay of the cytotoxin from Naja naja atra venom. The horse-peroxidase was used as the marker and polystyrene microhemagglutination plates as solid carrier. The sensitivity was 0.5 ng.ml-1. The assay range was 1.25-320 ngml-1 with coefficient of variation of 4.9% (intragroup) and 8.1% (intergroups), respectively.
Guo
M P
MP
Laboratory of Snake Venom Research, Fujian Medical College, Fuzhou, China.
Wang
Q C
QC
Liu
G F
GF
D000818:Animals; D001360:Avidin; D001710:Biotin; D003603:Cytotoxins / Q000032:analysis*; D004546:Elapid Venoms / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods
D003603:Cytotoxins; D004546:Elapid Venoms; D001360:Avidin; D001710:Biotin