Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression.
Jasmonic acid (JA) and its methyl ester, methyl jasmonate (MeJA), are plant lipid derivatives that resemble mammalian eicosanoids in structure and biosynthesis. These compounds are proposed to play a role in plant wound and pathogen responses. Here we report the quantitative determination of JA/MeJA in planta by a procedure based on the use of [13C,2H3]MeJA as an internal standard. Wounded soybean (Glycine max [L] Merr. cv. Williams) stems rapidly accumulated MeJA and JA. Addition of MeJA to soybean suspension cultures also increased mRNA levels for three wound-responsive genes (chalcone synthase, vegetative storage protein, and proline-rich cell wall protein) suggesting a role for MeJA/JA in the mediation of several changes in gene expression associated with the plants' response to wounding.
Creelman
R A
RA
Biotechnology Center, Ohio State University, Columbus 43210.
Tierney
M L
ML
Mullet
J E
JE
D000217:Acyltransferases / Q000235:genetics; D002473:Cell Wall; D003517:Cyclopentanes / Q000737:chemistry; D005786:Gene Expression Regulation; D013058:Mass Spectrometry; D054883:Oxylipins; D014674:Plant Proteins, Dietary / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013025:Glycine max / Q000235:genetics / Q000502:physiology*; D014945:Wound Healing
D003517:Cyclopentanes; D054883:Oxylipins; D014674:Plant Proteins, Dietary; D012333:RNA, Messenger; C011006:jasmonic acid; D000217:Acyltransferases; C020141:flavanone synthetase