title: Determination of oxytetracycline in blood serum by high-performance liquid chromatography with direct injection.
abstract: A direct injection analysis by high-performance liquid chromatography has been developed for oxytetracycline in serum of animals and fish. A Hisep shielded hydrophobic phase column (15 cm x 4.6 mm I.D.) and a mobile phase of methanol-0.2 M oxalic acid (10:90, v/v, pH 7.0) with ultraviolet detection at 360 nm were used. The standard calibration curves in serum of chicken, hog, cattle and rainbow trout were linear over the range 0.1-20 micrograms/ml. The recoveries of oxytetracycline from all serum samples determined at two different concentrations (0.5 and 2.0 micrograms/ml) were 88-103%. The detection limit was 0.05 micrograms/ml for every serum sample.
authors.affiliation: Faculty of Bioresources, Mie University, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Ueno
authors.forename: K
authors.initials: K
authors.lastname: Uno
authors.forename: T
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D010118:Oxytetracycline / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D014337:Trout
chemical_list: D010118:Oxytetracycline