Abnormal expression of neurofilament proteins in Ewing's sarcoma cell cultures.
A neural origin of Ewing's sarcoma (ES) has often been suggested and we have demonstrated neurofilament protein expression in ES cells. However, only the 200-kD subunit has been revealed in all of the ES cells analyzed. The 160- and 68-kD subunits were always absent. For these reasons, we have attempted to induce neural differentiation in 3 ES cell lines with different types of inducers: tetradecanoylphorbol-13-acetate (TPA) retinoic acid and nerve growth factor. When the cell lines were cultured for 7 days with TPA (10(-9) M) or retinoic acid (10(-7) M), only the 68-kD neurofilament subunit was slightly induced. No inducation was obtained when nerve growth factor was used, even at a 21-day culture. These results are in agreement with the putative neural origin of ES and may indicate an abnormal expression of neurofilament proteins in this tumor.
Lizard-Nacol
S
S
Centre Georges-François-Leclerc, Dijon, France.
Volk
C
C
Lizard
G
G
Turc-Carel
C
C
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008854:Microscopy, Electron; D016900:Neurofilament Proteins / Q000096:biosynthesis*; D012512:Sarcoma, Ewing / Q000378:metabolism* / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D016900:Neurofilament Proteins; D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate