Proteoglycans in human burn hypertrophic scar from a patient with Ehlers-Danlos syndrome.
Proteoglycans (PGs) from human burn hypertrophic scar of a patient with Ehlers-Danlos syndrome were extracted with 4M guanidinium chloride and purified by DEAE-cellulose chromatography. Differential ethanol precipitation of the PG fraction obtained after ion-exchange chromatography yielded two low mol.-wt. PGs, on rich in glucuronic acid (PGGLCA; Mr 66 kDa) and the other rich in iduronic acid (PGIDOA; Mr 48 kDa). In PGGLCA, 84% of the glycosaminoglycan chains are composed of GlcA----GalNAc(SO4) units, whereas in PGIDOA, the chains contain 95% IdoA----GalNAc(SO4) disaccharide units. Upon treatment with testicular hyaluronidase, the PGs gave different-sized oligosaccharides. Chondroitinase ABC digestion of PGGLCA or PGIDOA gave a single protein core (Mr approximately 20 kDa). The presence of glucosamine and sialic acid in PGGLCA and PGIDOA suggests that both contain N-linked oligosaccharides.
Garg
H G
HG
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA.
Lippay
E W
EW
Neame
P J
PJ
D000596:Amino Acids / Q000032:analysis; D002056:Burns / Q000150:complications / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002648:Child; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D002921:Cicatrix / Q000378:metabolism* / Q000473:pathology; D004535:Ehlers-Danlos Syndrome / Q000150:complications / Q000378:metabolism*; D004588:Electrophoresis, Cellulose Acetate; D006025:Glycosaminoglycans / Q000302:isolation & purification; D006801:Humans; D006984:Hypertrophy; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification
D000596:Amino Acids; D006025:Glycosaminoglycans; D011509:Proteoglycans