Lyn, a src-like tyrosine-specific protein kinase, is expressed in HL60 cells induced to monocyte-like or granulocyte-like cells.
During the in vitro differentiation of HL60 cells tyrosine-specific kinases are activated. The expression of lyn, a src-related tyrosine kinase, was studied by analysis of the steady-state levels of its transcript during the cell differentiation process induced by retinoic acid, phorbol 12-myristate 13-acetate and 1,25-dihydroxyvitamin D3. In contrast to an earlier report we observe only a small induction of the lyn-RNA levels compared to uninduced control cells. In unstimulated HL60 cells, the level for the lyn-transcript was comparatively high. A second, minor human lyn-transcript with an estimated size of 3.7 kb which has not been previously described, was identified.
Meier
R W
RW
Laboratory of Clinical and Experimental Research, University of Berne, Tiefenau Hospital, Switzerland.
Bielke
W
W
Chen
T
T
Niklaus
G
G
Friis
R R
RR
Tobler
A
A
D002454:Cell Differentiation; D006098:Granulocytes / Q000201:enzymology; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000201:enzymology*; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D009000:Monocytes / Q000201:enzymology; D005810:Multigene Family / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases