Capillary electrophoretic analysis of flavonoids.
Combining the effects of electrophoresis and electroendosmosis, flavonoids were separated in less than ten minutes in a fused silica capillary tube with a borate buffer adjusted to pH 10. An increase in the concentration of borate from 0.1 to 0.2 M resulted in longer migration times due to a decrease in electroosmotic flow, but also in improved selectivity and higher resolution of flavonoids. The calibration curve of rutin showed a detection limit of 0.02 mg/mL and linearity over its pharmaceutical concentration range. Using an internal standard of known concentration, the content of rutin in a methanolic extract of Sambuci flos could be determined with a coefficient of variation as small as 3.8% by the molar ratio-peak area ratio method.
Seitz
U
U
Institute of Radiochemistry, University of Innsbruck, Austria.
Oefner
P J
PJ
Nathakarnkitkool
S
S
Popp
M
M
Bonn
G K
GK
D001888:Boric Acids; D004586:Electrophoresis / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis; D012431:Rutin / Q000032:analysis*; D013997:Time Factors
D001888:Boric Acids; D005419:Flavonoids; D010936:Plant Extracts; D012431:Rutin; C032688:boric acid