title: An investigation of the contribution of the extraosseous tissues to the diaphyseal fracture callus using a rabbit tibial fracture model and in situ immunocytochemical localisation of osteocalcin.
abstract: The extraosseous tissue contribution to diaphyseal fracture callus has been investigated using a rabbit tibial fracture model and osteocalcin immunocytochemistry. The extraosseous tissues were isolated for study by reaming and nailing an osteotomy and excising 2 cm of periosteum on either side of the osteotomy. Specimens obtained from the healing fractures at 1 and 2 weeks after operation, respectively, were decalcified and stained for osteocalcin, a bone-specific protein, using an indirect immunoperoxidase method. The positively stained osteogenic cells appeared to be derived exclusively from the remnant of the periosteum.
authors.affiliation: University Department of Orthopaedic Surgery, Glenfield General Hospital, Leicester, England.
authors.forename: H
authors.initials: H
authors.lastname: Stafford
authors.forename: O O
authors.initials: OO
authors.lastname: Oni
authors.forename: J
authors.initials: J
authors.lastname: Hay
authors.forename: P J
authors.initials: PJ
authors.lastname: Gregg
mesh_terms: D000818:Animals; D002146:Bony Callus / Q000276:immunology / Q000503:physiopathology*; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards*; D008297:Male; D015675:Osteocalcin / Q000096:biosynthesis* / Q000276:immunology; D010784:Photomicrography; D011817:Rabbits; D013978:Tibial Fractures / Q000276:immunology / Q000503:physiopathology*; D014945:Wound Healing
chemical_list: D015675:Osteocalcin