Measurement of conjugated 1 beta-hydroxycholic acid in urine of newborns by specific radioimmunoassay.
Anti-tauro 1 beta-hydroxycholic acid antisera were prepared by immunizing rabbits with N-(1 beta,3 alpha,7 alpha,12 alpha-tetrahydroxy-5 beta-cholan-24-oyl)glycine bovine serum albumin conjugate. The immunoglobulin G fraction was obtained by ammonium sulfate precipitation, followed by diethylaminoethyl cellulose column chromatography. The antibody was characterized using [2-3H]tauro 1 beta-hydroxycholic acid which has a high affinity (Ka = 1.09 x 10(9) M-1) and reasonable specificity. Cross-reactivity for glyco 1 beta-hydroxycholic acid was 100% and those for other 1 beta-hydroxylated bile acids ranged from 4.32 to 29.6%. Concentrations of conjugated 1 beta-hydroxycholic acid in urine of newborns at 0-20 d after birth were determined by radioimmunoassay to be significant (0.2-11.1 micrograms/ml), exhibiting a tendency to increase during the 20 d after birth.
Ikegawa
S
S
Faculty of Pharmaceutical Sciences, Higashi-Nippon-Gakuen University, Hokkaido, Japan.
Murai
T
T
Yoshimura
T
T
Xu
Z Z
ZZ
Tohma
M
M
D000818:Animals; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007106:Immune Sera; D007231:Infant, Newborn; D011817:Rabbits; D011863:Radioimmunoassay; D013656:Taurocholic Acid / Q000031:analogs & derivatives* / Q000276:immunology / Q000652:urine
D007106:Immune Sera; C075168:tauro 1-hydroxycholic acid; D013656:Taurocholic Acid