Inhibition of transporter mediated gamma-aminobutyric acid (GABA) release by SKF 89976-A, a GABA uptake inhibitor, studied in a primary neuronal culture from chicken.
The effect of SKF 89976-A, a lipophilic non-substrate inhibitor of the gamma-aminobutyric acid (GABA) transporter, on the release of radioactive GABA and D-aspartate has been studied. Neuronal cultures from 8 day old chick embryos, grown for six days, served as a model. The cultures were incubated with [3H] D-aspartate and [14C] GABA with the subsequent addition of high or low concentrations of SKF 89976-A. Finally the cultures were exposed to differently composed media for either 30 or 300 seconds. The release was quantified, using liquid scintillation counting. The efflux of [3H] D-aspartate and [14C] GABA was increased by [K+] and time, and a minimum value was obtained at [Ca2+] 1.05 mM. The release of both [3H] D-aspartate and [14C] GABA was inhibited by SKF 89976-A. The obtained results indicate that transporter mediated processes are the major mechanisms of transmitter release in the investigated model.
Lewin
L
L
Dept. of Zoophysiology, University of Umeå, Sweden.
Mattsson
M O
MO
Sellström
A
A
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009557:Nipecotic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D012044:Regression Analysis; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D009557:Nipecotic Acids; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; C045598:N-(4,4-diphenyl-3-butenyl)nipecotic acid; D011188:Potassium