title: The M RNA of impatiens necrotic spot Tospovirus (Bunyaviridae) has an ambisense genomic organization.
abstract: The nucleotide sequence of Impatiens necrotic spot virus (INSV) M RNA was determined from cDNA clones. The INSV M RNA was 4972 nucleotides in length with two open reading frames (ORFs) in an ambisense genomic organization. The larger ORF near the 3' end of the viral RNA, coding for a protein with a predicted molecular weight of 124.9 kDa, was in the viral complementary sense and produced the G2 and G1 proteins. A smaller ORF in the viral sense was capable of coding for a 34.1-kDa polypeptide, designated the NSm protein. Two subgenomic RNA species were detected in INSV-infected tissue that corresponded to the predicted sizes (3.3 and 1.0 kb) of the G2-G1 and NSm mRNAs. The ORFs were separated by a 478 nucleotide A-U-rich intergenic region similar to the regions found in other viral RNAs with ambisense ORFs. The intergenic region was predicted to form a stable stem-loop structure (-81.2 kcal/mole). The ambisense genomic organization is characteristic of the S RNA for members of the Phlebovirus, Uukuvirus, and Tospovirus genera in the Bunyaviridae family. This is the first report of an ambisense Bunyaviridae M RNA.
authors.affiliation: Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616.
authors.forename: M D
authors.initials: MD
authors.lastname: Law
authors.forename: J
authors.initials: J
authors.lastname: Speck
authors.forename: J W
authors.initials: JW
authors.lastname: Moyer
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002043:Bunyaviridae / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins; D004247:DNA