title: Electron-capture gas chromatographic procedure for simultaneous determination of amphetamine and N-methylamphetamine.
abstract: An electron-capture gas chromatographic procedure for the simultaneous determination of amphetamine and N-methylamphetamine in biological samples is described. The method employs extraction with the ion-pairing reagent bis(2-ethylhexyl)phosphoric acid, and back-extraction with 0.5 M hydrochloric acid. The hydrochloric acid phase is basified, and the amphetamines and the internal standard benzylamine are derivatized with pentafluorobenzenesulfonyl chloride prior to analysis on a gas chromatograph equipped with a capillary column. Levels of amphetamine and N-methylamphetamine have been determined in the urine and liver of rats treated chronically with (-)-deprenyl.
authors.affiliation: Department of Psychiatry, University of Alberta, Edmonton, Canada.
authors.forename: P R
authors.initials: PR
authors.lastname: Paetsch
authors.forename: G B
authors.initials: GB
authors.lastname: Baker
authors.forename: L E
authors.initials: LE
authors.lastname: Caffaro
authors.forename: A J
authors.initials: AJ
authors.lastname: Greenshaw
authors.forename: G A
authors.initials: GA
authors.lastname: Rauw
authors.forename: R T
authors.initials: RT
authors.lastname: Coutts
mesh_terms: D000661:Amphetamine / Q000378:metabolism* / Q000652:urine; D000818:Animals; D002849:Chromatography, Gas; D004583:Electrons; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000737:chemistry*; D008297:Male; D008694:Methamphetamine / Q000378:metabolism* / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D012642:Selegiline / Q000008:administration & dosage
chemical_list: D012642:Selegiline; D008694:Methamphetamine; D000661:Amphetamine