Reinfusion of autogenous ascitic fluid concentrated by freezing in patients with intractable ascites.
Eleven patients with intractable ascites were treated by intravenous reinfusion of concentrated autogenous ascitic fluid. The ascitic fluid was drained and centrifuged for removal of cells by a cell separator. Cell-free ascitic fluid was frozen in a medical freezer at -15 degrees C and thawed at room temperature. The fraction at the initial stage of melting contains a high concentration of protein and was thus collected and reinfused to the patient. In this initial fraction, the recovery rate of albumin was 42.0 +/- 9.0% and the concentration of protein was 7.1 +/- 2.4 g/dl. This frozen method is inexpensive and time saving, because it does not use a filter. Ascites was ameliorated in all patients with 1 to 19 times of reinfusion. Therefore, when renal function is not disturbed, the frozen method may be indicated for management of intractable ascites.
Chiyoda
S
S
Department of Internal Medicine, Japanese Red Cross Nagasaki Atomic Bomb Hospital.
Morikawa
T
T
Matsuo
K
K
Masuya
S
S
D000328:Adult; D000368:Aged; D001201:Ascites / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D001202:Ascitic Fluid / Q000378:metabolism* / Q000473:pathology; D001806:Blood Urea Nitrogen; D002469:Cell Separation; D004573:Electrolytes / Q000378:metabolism; D005260:Female; D005615:Freezing; D006801:Humans; D007262:Infusions, Intravenous; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000302:isolation & purification; D013396:Suction; D014527:Uric Acid / Q000378:metabolism
D004573:Electrolytes; D011506:Proteins; D014527:Uric Acid