Photo-immunotargeting with haematoporphyrin conjugates activated by a low-power He-Ne laser.
A combined method has been developed for selective cytolysis in vitro as well as in vivo using a photosensitizer haematoporphyrin-protein conjugate as the targeting molecule and low-power He-Ne laser (632.8 nm) irradiation in order to activate the sensitizer to its excited, toxic triplet energy state. The specificity of the procedure was demonstrated in vitro by purging a mixed cell population from one component, and in vivo in an animal (nude mice) xenograft tumour model, where human cancer cells were destroyed by the immunotargeting method using monoclonal-antibody-haematoporphyrin (mAb-HP) conjugate (a-PNAr-I mAbs, which bind to the cell surface antigens of gastric cancer cells) and soft laser irradiation. The cell destruction was dependent on the doses of both mAb-HP and He-Ne laser light energy, and occurred only in target cell populations.
Berki
T
T
Immunological and Biotechnological Laboratory, University Medical School of Pécs, Hungary.
Németh
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P
D000230:Adenocarcinoma / Q000188:drug therapy*; D000818:Animals; D006414:Hematoporphyrin Photoradiation / Q000379:methods*; D006801:Humans; D006825:Hybridomas; D000922:Immunotoxins / Q000737:chemistry; D007834:Lasers; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010047:Ovalbumin / Q000276:immunology; D011975:Receptors, Mitogen / Q000276:immunology; D013274:Stomach Neoplasms / Q000188:drug therapy*
D000922:Immunotoxins; D011975:Receptors, Mitogen; C038223:peanut agglutinin receptor; D010047:Ovalbumin