title: Numerical chromosome anomalies after fertilization of freeze-thawed mouse oocytes.
abstract: The chromosome complement of first cleavage stage mouse embryos was analyzed to investigate the effect of slow freezing-fast thawing cryopreservation on chromosome numbers by comparing these numbers with those found fresh after fertilization of control oocytes. Fewer frozen-thawed (34.1%) than control oocytes (75.0%) cleaved to the 2-cell stage after in vitro fertilization. The incidence of hyperploidy was significantly increased by freezing (4.5% vs. 0% in controls). Polyploidy was not significantly affected (17.0% for freeze-thaw embryos vs. 26.2% for controls).
authors.affiliation: Department of Gynecology and Obstetrics, University of Ulm, FRG.
authors.forename: K
authors.initials: K
authors.lastname: Sterzik
authors.forename: B
authors.initials: B
authors.lastname: Rosenbusch
authors.forename: D
authors.initials: D
authors.lastname: Grab
authors.forename: A
authors.initials: A
authors.lastname: Wahl
authors.forename: H M
authors.initials: HM
authors.lastname: Beier
authors.forename: C
authors.initials: C
authors.lastname: Lauritzen
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002869:Chromosome Aberrations / Q000235:genetics*; D025063:Chromosome Disorders; D015925:Cryopreservation; D004622:Embryo, Mammalian / Q000648:ultrastructure; D005260:Female; D005307:Fertilization in Vitro; D051379:Mice; D008807:Mice, Inbred BALB C; D009865:Oocytes