title: The complete nucleotide sequence of RNA 1 of a German isolate of barley yellow mosaic virus and its comparison with a Japanese isolate.
abstract: The nucleotide sequence of RNA 1 of a German isolate of barley yellow mosaic virus has been determined and compared with a Japanese isolate of the same virus. The sequence identity is 93.6% at the nucleotide level and 96% at the amino acid level. Similar values have been found for the polyproteins of the RNA 2 of both isolates (95%). Both isolates show an RNA 1-encoded protein arrangement similar to that of potyviruses such as tobacco etch virus. In contrast, the polyproteins of the small RNAs (RNA 2) do not show such a similarity to the polyproteins of other potyviruses. However, there is a striking difference between the two isolates in the generally highly conserved active site of the RNA-dependent RNA polymerase. The German isolate exactly matches the consensus sequences for previously described potyviral RNA-dependent RNA polymerases, whereas the Japanese isolate does not.
authors.affiliation: Max-Planck-Institut für Züchtungsforschung, Abteilung Genetische Grundlagen der Pflanzenzüchtung, Köln, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Peerenboom
authors.forename: M
authors.initials: M
authors.lastname: Pröls
authors.forename: J
authors.initials: J
authors.lastname: Schell
authors.forename: H H
authors.initials: HH
authors.lastname: Steinbiss
authors.forename: A D
authors.initials: AD
authors.lastname: Davidson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000235:genetics; D005858:Germany; D001467:Hordeum / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004277:DNA, Single-Stranded; D012367:RNA, Viral