[The effect of the calf spleen and calf thymus extracts, thymopentin and tuftsin, on the phagocytosis activity of neutrophilic granulocytes].
In the present in vitro study, the influence of proteolytic as well as untreated spleen and thymus extracts and, as a comparison, of biologically active peptides tuftsin and thymopentin on the phagocytic activities of human polymorphonuclear leukocytes was investigated. At a chosen concentration of 200 micrograms/ml, all products studied exhibited a significant stimulation of polymorphonuclear leukocyte activity, when lucigenin-amplified chemiluminescence was measured. This effect was considerably enhanced with tuftsin, thymopentin and spleen extract, compared to the thymus preparations. No difference in chemiluminescence was obvious between hydrolyzed and untreated thymus extracts. In parallel experiments, phagocytosis of opsonized zymosan was investigated by light microscopy. Using identical substrate concentrations, only spleen extract showed significant stimulation. This observation indicates that the investigated polymorphonuclear leukocyte activities may be caused by substrate-specific mechanism on the cellular level.
Krasowski
H
H
Institut für Chemie, Universität Hohenheim, Stuttgart-Hohenheim.
Stehle
P
P
Fürst
P
P
Kraus
W
W
D000328:Adult; D000818:Animals; D002417:Cattle; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D016305:Thymopentin / Q000494:pharmacology*; D014405:Tuftsin / Q000494:pharmacology*; D015054:Zymosan / Q000494:pharmacology
D015054:Zymosan; D016305:Thymopentin; D014405:Tuftsin
Einfluss von Kälbermilz- und Kälberthymusextrakten, Thymopentin und Tuftsin auf die Phagozytoseaktivität von neutrophilen Granulozyten.