Identification of alpha- and beta-species of calcitonin gene-related peptide in the rat amygdala after separation with capillary zone electrophoresis.
Calcitonin gene-related peptide (CGRP) may be present in two forms in nervous tissue. Reversed-phase high-performance liquid chromatography has previously been found to be insufficient to clearly separate alpha-CGRP and beta-CGRP. A method for the separation of CGRPs by capillary zone electrophoresis has been developed. Separation of human or rat alpha-CGRP and beta-CGRP was achieved at pH values between 3.5 and 4.5 and a potential of 20 kV in a fused-silica capillary. Electrophoresis of an extract of rat amygdala in a micropreparative way, with subsequent radioimmunoassay, revealed for the first time the presence of alpha-CGRP and beta-CGRP in this brain area. The method may thus be used for separation of CGRPs, to reveal the distribution of alpha-CGRP and beta-CGRP, and for purity control.
Saria
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Department of Psychiatry, University Hospital Innsbruck, Austria.
D000595:Amino Acid Sequence; D000679:Amygdala / Q000737:chemistry*; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000737:chemistry / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet
D015740:Calcitonin Gene-Related Peptide