Tumorigenic activity of a rearranged c-myc gene from a human T-cell leukemia line.
The T-lymphoma cell line Hut78 contains a rearranged c-myc oncogene derived from a translocation between the long arms of chromosomes 8 and 2; the event deletes the 3' end of the gene, causing the loss of the transcribed AT-rich sequence. It has recently been shown that the mutant c-myc mRNA is several-fold more stable than normal c-myc mRNA. We have assessed the tumorigenicity of the mutant c-myc allele by transfecting this gene and its normal counterpart into NIH3T3 cells, together with a neomycin resistance gene. Following selection for G-418 resistance, the cells were injected into nude mice. Tumors containing integrated c-myc arose in animals injected with cells transfected by the mutated, but not by the normal, allele. The results suggest that this rearranged c-myc bears a tumorigenic activity not observed in other naturally occurring mutated c-myc alleles and may have directly contributed to the tumorigenic event in the Hut78 cell line.
Petroni
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D
Dipartimento di Scienze e Tecnologie Biomediche, Università di Milano, Italy.
Comi
P
P
Giglioni
B
B
Stacchini
A
A
Martinotti
G
G
Guerrasio
A
A
Saglio
G
G
D016475:3T3 Cells; D000818:Animals; D002889:Chromosomes, Human, Pair 2; D002898:Chromosomes, Human, Pair 8; D015321:Gene Rearrangement / Q000235:genetics*; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D016399:Lymphoma, T-Cell / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D014162:Transfection; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured