Elevated temperature alters the ionic dependence of amine-induced pacemaker activity in a conditional burster neuron.
The anterior burster neuron of the lobster (Panulirus interruptus) stomatogastric ganglion is a conditional burster that functions as the primary pacemaker for the pyloric motor network. When modulatory inputs to this cell are blocked, it loses its bursting properties and becomes quiescent. Applications of the monoamines, dopamine, octopamine or serotonin restore rhythmic bursting in this cell (Flamm and Harris-Warrick 1986). At 15 degrees C, serotonin- and octopamine-induced oscillations depend critically upon sodium entry (blocked by low sodium saline or tetrodotoxin); dopamine-induced oscillations depend upon calcium entry (blocked by reduced extracellular calcium; Harris-Warrick and Flamm 1987). We show here that the ionic dependence of amine-induced oscillations in the anterior burster cell differs at 15 and 21 degrees C. At 21 degrees C, all amines have the potential to induce rhythmic oscillations in saline containing tetrodotoxin. At the elevated temperature and in tetrodotoxin, both calcium and sodium currents are essential for the maintenance of dopamine-induced oscillations; serotonin-induced oscillations do not depend upon either calcium or sodium alone; octopamine-induced oscillations do not depend upon calcium and show a variable dependence upon sodium. Thus, multiple ionic mechanisms, which vary with both the modulator and the ambient temperature, can be recruited to support rhythmic activity in a conditional burster neuron.
Johnson
B R
BR
Section of Neurobiology and Behavior, Cornell University, Ithaca, NY 14853.
Peck
J H
JH
Harris-Warrick
R M
RM
D000818:Animals; D001679:Biogenic Amines / Q000502:physiology*; D001683:Biological Clocks / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D004298:Dopamine / Q000502:physiology; D006358:Hot Temperature; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology; D008121:Nephropidae / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009655:Octopamine / Q000502:physiology; D012701:Serotonin / Q000502:physiology; D012964:Sodium / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology
D001679:Biogenic Amines; D009655:Octopamine; D012701:Serotonin; D013779:Tetrodotoxin; D012964:Sodium; D008274:Magnesium; D002118:Calcium; D004298:Dopamine