The effect of tunicamycin on the protease activity of GP63 from Leishmania major.
The protease activity of gp63 from L. major was studied in relation to tunicamycin induced N-deglycosylation. It was found that after tunicamycin treatment, a N-deglycosylated product of gp63 with protease activity is present at the cell surface of Leishmania promastigote.
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Departamento de Biologia Celular, Facultad de Ciencias, Universidad Central de Venezuela, Caracas.
Manenti
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Hernandez
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D000581:Amidohydrolases; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D007895:Leishmania tropica / Q000201:enzymology*; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008666:Metalloendopeptidases / Q000187:drug effects*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D012995:Solubility; D014415:Tunicamycin / Q000494:pharmacology*
D014415:Tunicamycin; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase