title: MHC restriction of synovial fluid lymphocyte responses to the triggering organism in reactive arthritis. Absence of a class I-restricted response.
abstract: Synovial fluid mononuclear cells (SFMC) from patients with reactive arthritis (ReA) show marked proliferative responses to preparations of the organism triggering the arthritis. Initial studies with MHC-specific MoAbs have indicated that a significant element of these proliferative responses is mediated by class II MHC-restricted CD4+ T cells. It is imperative to establish the presence or absence of a class I-restricted response, for two reasons. Firstly, the association of ReA with the MHC class I molecule, HLA B27, raises the possibility of there being a B27-restricted response to the triggering organism. Secondly, a number of the organisms associated with ReA are intracellular pathogens, whose antigens might be expected to be presented by class I MHC molecules. In an effort to identify a class I MHC-restricted pathogen-specific response in the SFMC of ReA patients, we have assessed the proliferative responses of SFMC depleted of CD4+ T cells. Responses were grossly diminished by CD4+ T cell depletion. We also investigated Chlamydia-specific cytotoxicity in the SFMC of patients with sexually acquired ReA in a system using productive chlamydial infection to produce both targets and effectors. Significant antigen specific cytotoxicity was not seen. These experiments do not provide evidence to support the existence of pathogen-specific responses by CD8+, class I-restricted synovial fluid T cells in ReA.
authors.affiliation: Department of Rheumatology, University of Birmingham, UK.
authors.forename: A B
authors.initials: AB
authors.lastname: Hassell
authors.forename: D
authors.initials: D
authors.lastname: Pilling
authors.forename: D
authors.initials: D
authors.lastname: Reynolds
authors.forename: P F
authors.initials: PF
authors.lastname: Life
authors.forename: P A
authors.initials: PA
authors.lastname: Bacon
authors.forename: J S
authors.initials: JS
authors.lastname: Gaston
mesh_terms: D016918:Arthritis, Reactive / Q000209:etiology / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002690:Chlamydia Infections / Q000150:complications / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000502:physiology; D006801:Humans; D009980:Influenza A virus; D007376:Interleukin-2; D008212:Lymphocyte Depletion; D008214:Lymphocytes / Q000276:immunology* / Q000502:physiology; D008285:Major Histocompatibility Complex / Q000276:immunology*; D008297:Male; D010835:Phytohemagglutinins; D000091402:Prohibitins; D013582:Synovial Fluid / Q000166:cytology*; D014373:Tuberculin
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; C000717331:PHB2 protein, human; D010835:Phytohemagglutinins; D000091402:Prohibitins; D014373:Tuberculin