title: Isolation of Pseudomonas pickettii strains that degrade 2,4,6-trichlorophenol and their dechlorination of chlorophenols.
abstract: Three strains of Pseudomonas pickettii that can grow with 2,4,6-trichlorophenol (2,4,6-TCP) as the sole source of carbon and energy were isolated from different mixed cultures of soil bacterial populations that had been acclimatized to 2,4,6-TCP. These strains released 3 mol of chloride ion from 1 mol of 2,4,6-TCP during the complete degradation of the TCP. Of these strains, P. pickettii DTP0602 in high-cell-density suspension cultures dechlorinated various chlorophenols (CPs). Cells that were preincubated with 2,4,6-TCP converted isomers of 4-CP to the corresponding chloro-p-hydroquinones, but those preincubated with 4-CP converted CPs lacking a chlorine atom(s) at the o position to isomers of chlorocatechol. The ability of DTP0602 to dechlorinate 2,4,6-TCP was induced by 2,6-dichlorophenol, 2,3,6- and 2,4,6-TCP, and 2,3,4,6-tetrachlorophenol and was repressed in the presence of succinate or glucose.
authors.affiliation: Biotechnology Laboratory, Department of Applied Chemistry, Okayama University of Science, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kiyohara
authors.forename: T
authors.initials: T
authors.lastname: Hatta
authors.forename: Y
authors.initials: Y
authors.lastname: Ogawa
authors.forename: T
authors.initials: T
authors.lastname: Kakuda
authors.forename: H
authors.initials: H
authors.lastname: Yokoyama
authors.forename: N
authors.initials: N
authors.lastname: Takizawa
mesh_terms: D001673:Biodegradation, Environmental; D002713:Chlorine / Q000378:metabolism; D002733:Chlorophenols / Q000378:metabolism* / Q000494:pharmacology; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D002733:Chlorophenols; D002713:Chlorine; C024564:2,4,6-trichlorophenol