title: SwaI, a unique restriction endonuclease from Staphylococcus warneri, which recognizes 5'-ATTTAAAT-3'.
abstract: A novel class-II restriction endonuclease designated SwaI was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, pBR322 or pBR328 DNA. SwaI recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. SwaI was used to digest chromosomal DNA from various microorganisms and human cells.
authors.affiliation: Boehringer Mannheim GmbH, Biochemical Research Center, Penzberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Lechner
authors.forename: B
authors.initials: B
authors.lastname: Frey
authors.forename: F
authors.initials: F
authors.lastname: Laue
authors.forename: J
authors.initials: J
authors.lastname: Anton-Botella
authors.forename: C L
authors.initials: CL
authors.lastname: Smith
authors.forename: W
authors.initials: W
authors.lastname: Ankenbauer
authors.forename: G G
authors.initials: GG
authors.lastname: Schmitz
mesh_terms: D004247:DNA / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D013210:Staphylococcus / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004247:DNA; C074767:endodeoxyribonuclease SwaI; D015252:Deoxyribonucleases, Type II Site-Specific