Expression of the polymeric immunoglobulin receptor by cultured aged rat hepatocytes.
The Fischer rat shows an age-related loss of both hepatic blood to bile transport and secretory component-specific binding sites for polymeric immunoglobulin (Ig) A. This age-related loss of hepatic IgA receptor function is also shown by cultured hepatocytes. It is reported here that compared with young cells, binding and uptake of 125I-polymeric IgA by cultured hepatocytes was markedly reduced in cells from senescent animals. In addition, cells from old animals showed markedly diminished secretion of secretory component determined by enzyme-linked immunosorbent assay and expression of polymeric immunoglobulin receptor determined by incorporation of 35S-labeled amino acid and fluorography. It is suggested that the age-related decrease in IgA receptor-mediated transport from serum to bile results, at least in part, from decreased expression and secretion of total hepatic secretory component.
Gregoire
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CD
Department of Pharmaceutical Sciences, College of Pharmacy, Idaho State University, Pocatello.
Zhang
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L
Daniels
C K
CK
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003545:Cysteine / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007070:Immunoglobulin A / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D011108:Polymers; D051381:Rats; D011916:Rats, Inbred F344; D011961:Receptors, Fc; D011971:Receptors, Immunologic / Q000378:metabolism*; D012635:Secretory Component / Q000378:metabolism
C045281:IgA receptor; D007070:Immunoglobulin A; D011108:Polymers; D011961:Receptors, Fc; D011971:Receptors, Immunologic; D012635:Secretory Component; C034639:polymeric IgA; D003545:Cysteine