Population genetic evidence for species A, B, C and D of the Anopheles dirus complex in Thailand and enzyme electromorphs for their identification.
Mixtures of chromosomal forms A, B, C and D in natural populations of Anopheles dirus Peyton & Harrison sensu lato in Thailand show significant positive values of Wright's fixation index for six enzyme-electromorph loci. The mean value of FIS over all loci was found to be +0.28 (SD 0.02), with a range of +0.57 (Odh) to +0.10 (Idh-2). Partitioning electromorph data for the chromosomal forms reduces the mean FIS to 0.03 (SD 0.01), which suggests that positive assortative mating is a characteristic of each form. This supports the hypothesis that the chromosomal/electrophoretic forms A, B, C and D represent four distinct biological species within the An. dirus complex. An example is given of the use of enzyme electromorphs as a means of vector identification during a malaria entomological field study involving a mixture of An. dirus species A and D. Electromorph identifications of 323 sp. A and 161 sp. D were more than 98% correct when cross-referenced to specific DNA probes.
Green
C A
CA
Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Munstermann
L E
LE
Tan
S G
SG
Panyim
S
S
Baimai
V
V
D000483:Alleles; D000818:Animals; D000852:Anopheles / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004798:Enzymes / Q000032:analysis / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007303:Insect Vectors / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D007621:Karyotyping; D008288:Malaria / Q000635:transmission*; D011336:Probability; D013785:Thailand
D004798:Enzymes