[The effect of He-Ne laser radiation on the survival of HeLa cells subjected to ionizing radiation].
A monolayer of HeLa cells, at the stationary phase of growth, exposed to He-Ne laser radiation (632.8 nm; 100 J/m2) either 5 min or 60 min prior to gamma irradiation (0.1-10 Gy; 6.75 Gy/min), or 5 min after irradiation has been investigated. With a 5-min interval between irradiation sessions (both sequences) the survival curves are virtually the same as those for gamma-irradiated cells only. With He-Ne laser radiation delivered 60 min before gamma irradiation with doses exceeding 5 Gy, a fraction of radioresistant cells is identified whose D0 is almost twice as high as D0 of basic cell mass (3.6 and 1.7 Gy respectively. The survival curve becomes a two-component one. A hypothesis is proposed that He-Ne laser radiation activates, in some cells, the processes that promote the repair of radiation damages.
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D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006367:HeLa Cells / Q000528:radiation effects*; D006801:Humans; D007834:Lasers; D013997:Time Factors
Vliianie izlucheniia He-Le lazera na vyzhivaemost' kletok HeLa, podvergnutykh deĭstviiu ioniziruiushcheĭ radiatsii.