Optimization of a peak compression system for a remoxipride metabolite (FLA797) and its application to bioanalysis.
A peak compression system is optimized for FLA797 (I), a phenolic tertiary amine and a metabolite to the antipsychotic drug remoxipride. An application is described where this effect is used to give a 6-7-fold improvement of the quantification limit in an assay of I in plasma. The liquid chromatographic system is constructed so that the injection of I dissolved in a solution of a competing amine gives a very high and narrow analyte peak with an apparent efficiency of 1.5 x 10(6) plates/m. When the levels of I in plasma are determined, an internal standard, giving a normal isocratic peak, is added to the plasma sample before the extraction. Concentrations of I down to 0.5-1.0 nM can be determined with reasonable precision. FLA908, another phenolic remoxipride metabolite and a regioisomer to I, eluting as a normal isocratic peak, can be determined simultaneously although only at concentrations higher than 10-15 nM.
Nilsson
L B
LB
Bioanalysis, Astra Arcus AB, Södertälje, Sweden.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D051381:Rats; D012031:Refractometry; D015203:Reproducibility of Results; D012457:Salicylamides / Q000097:blood*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D012457:Salicylamides; C050313:FLA 797