title: Nucleotide sequence of nifD from Frankia alni strain ArI3: phylogenetic inferences.
abstract: The complete nucleotide sequence of the nifD gene encoding the alpha subunit of component I of nitrogenase from Frankia alni strain ArI3 was determined. The coding region is 1,458 bp in length and encodes a polypeptide of 486 residues with a predicted molecular weight of 53,500. Phylogenetic inferences with 12 complete published nifD sequences were drawn using a variety of approaches. Frankia nifD clusters with proteobacteria rather than with Clostridium pasteurianum, the other Gram-positive bacterium studied. Extant eubacterial nif genes seem to have at least three distinct evolutionary origins as a result of ancient gene duplications. Within the Gram-positive bacterial phylum, functional nif genes descend from different duplicates.
authors.affiliation: Laboratoire d'Ecologie Microbienne du Sol, Université Claude-Bernard-Lyon I, France.
authors.forename: P
authors.initials: P
authors.lastname: Normand
authors.forename: M
authors.initials: M
authors.lastname: Gouy
authors.forename: B
authors.initials: B
authors.lastname: Cournoyer
authors.forename: P
authors.initials: P
authors.lastname: Simonet
mesh_terms: D000192:Actinomycetales / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment
chemical_list: D004269:DNA, Bacterial; D009591:Nitrogenase