title: Molecular structure of the C beta catalytic subunit of rat cAMP-dependent protein kinase and differential expression of C alpha and C beta isoforms in rat tissues and cultured cells.
abstract: A full-length cDNA clone encoding the C beta catalytic subunit of cAMP-dependent protein kinase (PKA) was isolated from a rat brain cDNA library. A 1.1 kb cDNA containing the entire coding region encodes for a protein of 351 amino acids that shows more than 95% sequence homology to the C beta subunits in mouse, bovine and human. Northern blot analysis showed distinct patterns of C alpha and C beta mRNA expression in the brain and various peripheral tissues. The C alpha mRNA was widespread and highly expressed in brain, heart, adrenal gland, testis, lung, kidney, spleen and liver, whereas the C beta mRNA was unevenly expressed in the brain and adrenal gland and in much lesser amounts in other tissues. The C alpha mRNA was evenly distributed and highly expressed through various regions of the brain, while the C beta mRNA was expressed in lesser amounts and was unevenly distributed. In neuronal and glial cultured cells, C alpha mRNA was also predominantly expressed but C beta mRNA was undetectable. The differential distribution between the C subunit isoforms of PKA suggests that individual subunits are involved in specialized functions.
authors.affiliation: Department of Pharmacology, Kobe University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shuntoh
authors.forename: N
authors.initials: N
authors.lastname: Sakamoto
authors.forename: S
authors.initials: S
authors.lastname: Matsuyama
authors.forename: M
authors.initials: M
authors.lastname: Saitoh
authors.forename: C
authors.initials: C
authors.lastname: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009928:Organ Specificity / Q000235:genetics; D016716:PC12 Cells; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
chemical_list: D012333:RNA, Messenger; D004247:DNA; D011494:Protein Kinases