Localization by immunogold labelling of HIV-1 structural proteins on Lowicryl embedded HIV-1 infected cell ultrathin sections.
Using several HIV-1-specific antibodies and the immunogold labelling technique, we have detected and localized distinct viral proteins on ultrathin sections of HIV-1 infected cells embedded in the Lowicryl K4M resin. Monoclonal antibodies (MAbs) against p24, p17 and gp160/gp41 showed a preferential labelling on viral formations still attached to the cell membrane (budding process) or free in the extracellular space. The anti-p24 and the anti-p17 MAb yielded a gold labelling not only on mature but also on immature virions where the gold particles were associated with the ring-like electron-dense material. The three HIV-1-specific antibodies against p24, p17 and p55 yielded a cross-reaction with HIV-2 in agreement with the conservation of the internal antigenic determinants of both viruses. In all instances, there was no specific immunogold labelling over the cells, suggesting that once the virus structural proteins were synthesized, they were promptly utilized for virus assembly at the plasma membrane level.
Grigoriev
V B
VB
Ivanovsky Institute of Virology, Moscow, USSR.
Escaig-Haye
F
F
Sharova
N K
NK
Rudneva
I A
IA
Simonov
V
V
Kazennova
E K
EK
Kush
A A
AA
Klimenko
S M
SM
Buckrinskaya
A
A
Fournier
J G
JG
D000180:Acrylic Resins; D000911:Antibodies, Monoclonal; D006046:Gold; D015483:HIV Antibodies; D015658:HIV Infections / Q000382:microbiology*; D015497:HIV-1 / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D016253:Microscopy, Immunoelectron / Q000379:methods*; D008867:Microtomy; D014407:Tumor Cells, Cultured; D015678:Viral Structural Proteins / Q000302:isolation & purification*
D000180:Acrylic Resins; D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D015678:Viral Structural Proteins; D006046:Gold; C037883:Lowicryl 4KM resin