title: CD69 molecule in human neutrophils: its expression and role in signal-transducing mechanisms.
abstract: The CD69 glycoprotein is an early activation antigen of T and B lymphocytes and it is constitutively expressed on thymocytes and platelets. Here we report its presence on neutrophils and on bone marrow-derived myeloid precursors. Indeed, promyelocytic cells are CD69+ on the cell membrane, while in resting neutrophils this molecule is located inside the cell. However, intracellular CD69 molecules are rapidly mobilized to the cell surface upon activation by PMA or fMLP. This translocation is independent on a new protein synthesis, as it is not inhibited by cycloheximide; furthermore, CD69 molecules are likely stored in a trans-Golgi structure since their expression is not affected by brefeldin A, a drug that blocks molecular trafficking from ER to Golgi vesicles. Immunoprecipitation of CD69 molecules either from activated neutrophils or from bone marrow cells showed that this protein has the same molecular size (28-34 kDa) as observed in platelets, T and B lymphocytes, and thymocytes. This similarity is reflected also in the functional role played by this molecule: in neutrophils as well as in lymphocytes and platelets, CD69 stimulation induced Ca2+ influx through cellular membrane; furthermore, the perturbation of the CD69 antigen on PMA-activated neutrophils enhances the lysozyme release, suggesting a role of this molecule in the regulation of granule exocytosis, probably through a Ca(2+)-dependent mechanism.
authors.affiliation: Istituto di Chimica Biologica Università degli Studi di Ferrara, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Gavioli
authors.forename: A
authors.initials: A
authors.lastname: Risso
authors.forename: D
authors.initials: D
authors.lastname: Smilovich
authors.forename: I
authors.initials: I
authors.lastname: Baldissarro
authors.forename: M C
authors.initials: MC
authors.lastname: Capra
authors.forename: A
authors.initials: A
authors.lastname: Bargellesi
authors.forename: M E
authors.initials: ME
authors.lastname: Cosulich
mesh_terms: D015703:Antigens, CD / Q000032:analysis* / Q000502:physiology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis* / Q000502:physiology; D001854:Bone Marrow Cells; D006801:Humans; D037181:Lectins, C-Type; D008247:Lysosomes / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D010835:Phytohemagglutinins; D015398:Signal Transduction; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D037181:Lectins, C-Type; D009240:N-Formylmethionine Leucyl-Phenylalanine; D010835:Phytohemagglutinins; D000099192:CD69 Antigens