Induction of enzymes involved in fatty acid beta-oxidation in Pseudomonas fragi B-0771 cells grown in media supplemented with fatty acid.
Induction of the enzymes involved in fatty acid beta-oxidation in Pseudomonas fragi B-0771 cells grown in a medium containing straight chain saturated fatty acids was studied. The acyl-CoA dehydrogenase (ACDH) activity was induced during the exponential phase in cells grown in palmitic acid-supplemented medium, reached a maximum at the early stationary phase, and then gradually decreased thereafter. Changes in the overall activities of 2-enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase, both existing on the multienzyme complex (HDT) involved in fatty acid beta-oxidation, were similar to that in ACDH activity. Straight chain saturated fatty acids having more than 6 carbon atoms could induce both the ACDH and HDT activities, and C13-C15 fatty acids caused the greatest induction of both activities. Changes in the overall activities of 2-enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase correlated with that in the amount of the alpha-subunit of HDT during the entire culture period in the medium containing palmitic acid. Surprisingly, the stoichiometry of the alpha- and beta-subunit proteins of HDT was not maintained into the stationary phase culture, though the genes encoding the alpha- and beta-subunits are tandemly coded in bacterial genomic DNA.
Sato
S
S
Department of Biology, College of Arts and Sciences, University of Tokyo.
Imamura
S
S
Ozeki
Y
Y
Kawaguchi
A
A
D000102:Acetyl-CoA C-Acyltransferase / Q000096:biosynthesis*; D042964:Acyl-CoA Dehydrogenase; D044944:Acyl-CoA Dehydrogenases / Q000096:biosynthesis*; D015153:Blotting, Western; D002455:Cell Division; D003470:Culture Media; D004746:Enoyl-CoA Hydratase / Q000096:biosynthesis*; D004790:Enzyme Induction; D005227:Fatty Acids / Q000378:metabolism*; D010084:Oxidation-Reduction; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D003470:Culture Media; D005227:Fatty Acids; D010169:Palmitic Acids; D019308:Palmitic Acid; D044944:Acyl-CoA Dehydrogenases; D042964:Acyl-CoA Dehydrogenase; D000102:Acetyl-CoA C-Acyltransferase; D004746:Enoyl-CoA Hydratase