Use of colistin-polymyxin B-cellobiose agar for isolation of Vibrio vulnificus from the environment.
Colistin-polymyxin B-cellobiose agar was employed for the isolation of Vibrio vulnificus from shellfish. Isolates were examined phenotypically and with a gene probe and monoclonal antibody specific for V. vulnificus. Results indicated that colistin-polymyxin B-cellobiose agar is superior to both sodium dodecyl sulfate-polymyxin B-sucrose agar and thiosulfate-citrate-bile salts-sucrose agar in its ability to select and differentiate this species from background vibrios.
Oliver
J D
JD
Department of Biology, University of North Carolina, Charlotte 28223.
Guthrie
K
K
Preyer
J
J
Wright
A
A
Simpson
L M
LM
Siebeling
R
R
Morris
J G
JG
D000362:Agar; D001431:Bacteriological Techniques; D002475:Cellobiose; D003091:Colistin; D005069:Evaluation Studies as Topic; D010641:Phenotype; D011112:Polymyxin B; D012758:Shellfish / Q000382:microbiology*; D014733:Vibrio / Q000302:isolation & purification*
D002475:Cellobiose; D000362:Agar; D011112:Polymyxin B; D003091:Colistin