title: Detection of free radicals during brain ischemia and reperfusion by spin trapping and microdialysis.
abstract: Extracellular free radicals were detected in rat striatal perfusate samples by intracerebral microdialysis coupled to the spin trapping technique. Five Sprague-Dawley rats were subjected to 30 min of global ischemia followed by reperfusion; throughout the experimental period the intrastriatal dialysing probe was perfused with Ringer's solution containing the spin trap agent pyridyl-N-oxide-t-butylnitrone (100 mM) together with the iron chelating agent diethylentriaminepentacetic acid (100 microM). A radical adduct occurred during ischemia and early reperfusion, but not in basal conditions; the spin adduct was characterized as a carbon centered radical, consistent with the presence of an oxidative attack on membrane lipids. The direct evidence of the formation of free radicals supports the hypothesis that free radicals play a role in the pathogenesis of the histological damage during brain ischemia.
authors.affiliation: Institute of Human Physiology, University of Modena, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Zini
authors.forename: A
authors.initials: A
authors.lastname: Tomasi
authors.forename: R
authors.initials: R
authors.lastname: Grimaldi
authors.forename: V
authors.initials: V
authors.lastname: Vannini
authors.forename: L F
authors.initials: LF
authors.lastname: Agnati
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D003497:Cyclic N-Oxides; D003956:Dialysis / Q000379:methods; D005609:Free Radicals; D009589:Nitrogen Oxides; D011725:Pyridines; D051381:Rats; D011919:Rats, Inbred Strains; D015424:Reperfusion; D013113:Spin Labels; D013858:Thiobarbiturates
chemical_list: D003497:Cyclic N-Oxides; D005609:Free Radicals; D009589:Nitrogen Oxides; D011725:Pyridines; D013113:Spin Labels; D013858:Thiobarbiturates; C043438:alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone; C029217:phenyl-N-tert-butylnitrone; C017245:5,5-dimethyl-1-pyrroline-1-oxide; C029684:thiobarbituric acid