Two-step carcinogenesis induced by neonatal exposure to 5-bromo-2'- deoxyuridine and subsequent administration of urethan in BALB/c mice.
Male and female BALB/c mice were exposed to subcutaneous injections of 1 mg 5-bromo-2'-deoxyuridine (BrdUrd) at the 1st, 3rd and 7th days after their birth and/or starting from 3 months of age, and every third day were given 5 intraperitoneal injections of 0.1 ml of 5% solution of urethan. Treatment with BrdUrd alone followed by delay in mice development, reduction of the body and liver weight did not significantly increase the incidence of tumor development at 9 months of age. Exposure to both BrdUrd and urethan lead to summation or potentiation of their effects, manifested in increased lung adenoma incidence and their number per tumor-bearing mouse. In males, which received BrdUrd and urethan a significant increase in lung adenocarcinomas and haemoblastosis was observed in comparison to other groups. It was suggested that genome instability induced by exposure to BrdUrd facilitate the realization of the carcinogenic effect of urethan.
Anisimov
V N
VN
Laboratory of Experimental Tumors, N.N. Petrov Research Institute of Oncology, St. Petersburg, Russia.
Osipova GYu
D000230:Adenocarcinoma / Q000139:chemically induced*; D000236:Adenoma / Q000139:chemically induced*; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D003043:Cocarcinogenesis; D005260:Female; D008175:Lung Neoplasms / Q000139:chemically induced*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size; D014520:Urethane / Q000633:toxicity*
D014520:Urethane; D001973:Bromodeoxyuridine