title: Dendritic outgrowth of myenteric plexus neurons in primary culture.
abstract: Myenteric plexus neurons derived from neonatal guinea pigs, when exposed to serum, demonstrated a characteristic pattern of growth, including a proliferating outgrowth zone of glial cells, peripheral extension of dendritic processes, and progressive dendritic growth. Serum effects upon dendritic growth, measured morphometrically, was strongly dose- and temporally dependent. Dendritic density was increased 10-fold (120 hr) by the addition of 6% serum, while mean dendritic length was increased 3-fold. Development of cholinergic function was reflected by release of [3H]ACh in response to cholecystokinin octapeptide, vasoactive intestinal peptide, substance P, and calcitonin gene-related peptide (10(-10) and 10(-8) M).
authors.affiliation: Department of Surgery, University of Michigan, Ann Arbor 48109-0331.
authors.forename: M W
authors.initials: MW
authors.lastname: Mulholland
authors.forename: G
authors.initials: G
authors.lastname: Romanchuk
authors.forename: K
authors.initials: K
authors.lastname: Flowe
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure; D009197:Myenteric Plexus / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D009474:Neurons / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D000110:Acetylcholinesterase