[A study of the protective mechanism of hepatic stimulator substance against experimental acute liver failure in mice].
In our previous work we have demonstrated a protective effect of hepatic stimulator substance (HSS) on experimental hepatic injury in mice. The underlying mechanism of this effect was further investigated with the following results: (1) HSS could significantly increase the CCl4-induced decrease of membrane fluidity of hepatic plasmalemma, mitochondria and microsomes to the control level. (2) HSS could decrease the liver melondialdehyde contents of CCl4-intoxicated mice. (3) HSS could restore the liver glutathione contents lowered by CCl4-intoxication. (4) HSS stimulated regeneration of liver, enhanced DNA synthesis of hepatocytes and increased 3H-TdR incorporation into DNA. According to the results mentioned above, we proposed that HSS should have an antioxidative effect on the hepatic membrane lipid peroxidation which was increased by free radicals produced by CCl4. In addition, HSS might increase antioxidative ability of hepatocyte and stimulate the proliferation of hepatocytes. These protective mechanisms might act in a concordant manner.
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Department of Physiology, Dalian Medical College.
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D000818:Animals; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C073456:hepatic stimulator substance; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D005978:Glutathione