title: Poloxamer 188 improves neurologic outcome after hypothermic circulatory arrest.
abstract: Poloxamer 188, an amphipathic copolymer with cytoprotective properties, was investigated as a means of improving neurologic outcome after a prolonged period (150 minutes) of deep hypothermic circulatory arrest. Dogs were perfusion cooled and surface cooled to 10 degrees C, the heart was arrested for 150 minutes, and then the dogs were rewarmed and weaned from bypass. Seven dogs were treated with poloxamer 188 before and after deep hypothermic circulatory arrest. Six control dogs were treated with saline. Surviving dogs were evaluated for 1 week after deep hypothermic circulatory arrest for neurologic deficits or behavioral changes. Neurologic outcome was graded by the following system: grade 1, death within the observation period; grade 2, comatose; grade 3, holds head up; grade 4, sits up; grade 5, stands; grade 6, normal in both behavior and gait. There were no deaths in the seven poloxamer 188-treated animals versus three deaths in the six control dogs. Poloxamer 188-treated dogs also manifested significantly less neurologic dysfunction after deep hypothermic circulatory arrest than did the control group (p less than 0.003). This study shows that poloxamer 188 has a significant impact in improving neurologic outcome after exceptionally long periods of deep hypothermic circulatory arrest.
authors.affiliation: Department of Cardiothoracic Surgery, Mount Sinai Medical Center, New York, NY 10029.
authors.forename: C K
authors.initials: CK
authors.lastname: Mezrow
authors.forename: M
authors.initials: M
authors.lastname: Mazzoni
authors.forename: D
authors.initials: D
authors.lastname: Wolfe
authors.forename: H H
authors.initials: HH
authors.lastname: Shiang
authors.forename: R S
authors.initials: RS
authors.lastname: Litwak
authors.forename: R B
authors.initials: RB
authors.lastname: Griepp
mesh_terms: D000818:Animals; D004285:Dogs; D004338:Drug Combinations; D004353:Drug Evaluation, Preclinical; D006324:Heart Arrest, Induced; D007036:Hypothermia, Induced; D009420:Nervous System / Q000187:drug effects*; D009460:Neurologic Examination; D011060:Poloxalene / Q000494:pharmacology / Q000627:therapeutic use*; D011092:Polyethylene Glycols / Q000494:pharmacology / Q000627:therapeutic use*; D011184:Postoperative Period; D011897:Random Allocation
chemical_list: D004338:Drug Combinations; D011092:Polyethylene Glycols; D011060:Poloxalene