title: Purification of the sex steroid-binding protein from common carp (Cyprinus carpio) plasma.
abstract: 1. Sex steroid-binding protein was purified from common carp plasma. 2. Testosterone- and estradiol-binding activity existed at the same fraction eluted from gel Sepharose CL-2B, DEAE-Sephacel, hydroxylapatite and HPLC. 3. The molecular weight of the sex steroid-binding protein was 194,000. 4. At 50% displacement the order in which the steroids displaced [3H]testosterone bound to the binding protein was as follows: androstenedione greater than estradiol-17 beta greater than 11-deoxy-17-hydroxycorticosterone greater than 17 alpha-hydroxyprogesterone greater than progesterone greater than deoxycorticosterone greater than estrone greater than 11-ketotestosterone greater than 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one greater than androstenedione greater than pregnenolone greater than cortisone greater than cortisol.
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung, Republic of China.
authors.forename: C F
authors.initials: CF
authors.lastname: Chang
authors.forename: Y H
authors.initials: YH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002347:Carps / Q000097:blood*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008970:Molecular Weight; D012738:Sex Hormone-Binding Globulin / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D012738:Sex Hormone-Binding Globulin