Studies on the proteins involved in the interaction of high-density lipoprotein with isolated human small intestine epithelial cells.
Treatment of 125I-labelled high-density lipoprotein ([125I]HDL3) with monospecific polyclonal antibodies against apolipoproteins A-I and A-II resulted in a dose-dependent inhibition of the [125I]HDL3 binding to isolated human small intestine epithelial cells by 25% and 50%, respectively. Both antibodies also inhibited intracellular degradation of [125I]HDL3 by 80%. Treatment of enterocytes with polyclonal antibody against apolipoprotein A-I binding protein, a putative HDL receptor, inhibited both binding and degradation of [125I]HDL3 by these cells by 50%. Antibodies to apolipoprotein A-I, A-II and apo A-I-binding protein also inhibited [125I]HDL3 binding to cholesterol-loaded cells.
Sviridov
D D
DD
Institute of Experimental Cardiology, Cardiology Research Center, Moscow, Russia.
Ehnholm
C
C
Tenkanen
H
H
Pavlov MYu
Safonova
I G
IG
Repin
V S
VS
D000906:Antibodies / Q000276:immunology; D016632:Apolipoprotein A-I / Q000276:immunology / Q000378:metabolism*; D016633:Apolipoprotein A-II / Q000276:immunology / Q000378:metabolism*; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism*; D011257:Pregnancy Proteins; D019998:Racemases and Epimerases
D000906:Antibodies; D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D002352:Carrier Proteins; D008075:Lipoproteins, HDL; D011257:Pregnancy Proteins; D002784:Cholesterol; C052433:NAXE protein, human; D019998:Racemases and Epimerases