Primary structure of a Thomsen-Friedenreich-antigen-specific lectin, jacalin [Artocarpus integrifolia (jack fruit) agglutinin]. Evidence for the presence of an internal repeat.
Jacalin [Artocarpus integrifolia (jack fruit) agglutinin] is made up of two types of chains, heavy and light, with M(r) values of 16,200 +/- 1200 and 2090 +/- 300 respectively (on the basis of gel-permeation chromatography under denaturing conditions). Its complete amino acid sequence was determined by manual degradation using a 4-dimethylaminoazobenzene 4'-isothiocyanate double-coupling method. Peptide fragments for sequence analysis were obtained by chemical cleavages of the heavy chain with CNBr, hydroxylamine hydrochloride and iodosobenzoic acid and enzymic cleavage with Staphylococcus aureus proteinase. The peptides were purified by a combination gel-permeation and reverse-phase chromatography. The light chains, being only 20 residues long, could be sequenced without fragmentation. Amino acid analyses and carboxypeptidase-Y-digestion C-terminal analyses of the subunits provided supportive evidence for their sequence. Computer-assisted alignment of the jacalin heavy-chain sequence failed to show sequence similarity to that of any lectin for which the complete sequence is known. Analyses of the sequence showed the presence of an internal repeat spanning residues 7-64 and 76-130. The internal repeat was found to be statistically significant.
Mahanta
S K
SK
Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
Sanker
S
S
Rao
N V
NV
Swamy
M J
MJ
Surolia
A
A
D000595:Amino Acid Sequence; D003488:Cyanogen Bromide / Q000378:metabolism; D019811:Hydroxylamine; D006898:Hydroxylamines; D007369:Interferon Inducers / Q000737:chemistry* / Q000378:metabolism; D007463:Iodobenzoates; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D037121:Plant Lectins; D012091:Repetitive Sequences, Nucleic Acid; D012697:Serine Endopeptidases / Q000378:metabolism
D006898:Hydroxylamines; D007369:Interferon Inducers; D007463:Iodobenzoates; D037102:Lectins; D046911:Macromolecular Substances; D010455:Peptides; D037121:Plant Lectins; C043696:jacalin; D019811:Hydroxylamine; C008154:2-iodosobenzoic acid; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D003488:Cyanogen Bromide