title: Preliminary crystallographic analysis of murine macrophage inflammatory protein 2.
abstract: Macrophage inflammatory protein 2 (MIP-2) has been crystallized by vapor diffusion of an 11 mg/ml protein solution in 100 mM-ammonium acetate against 30 to 40% polyethylene glycol (average molecular mass of 3350 Da). The crystals belong to space group P2(1)2(1)2(1) and have unit cell dimensions of a = 42.7 A, b = 59.3 A, and c = 100.3 A. The molecular mass of the protein and volume of the unit cell suggest that there are four monomers in the asymmetric unit. A data set to 2.3 A has been collected, and the self-rotation function identifies the presence of a non-crystallographic 2-fold axis.
authors.affiliation: Laboratory of Medical Biochemistry, Rockefeller University, New York, NY 10021.
authors.forename: E
authors.initials: E
authors.lastname: Lolis
authors.forename: R M
authors.initials: RM
authors.lastname: Sweet
authors.forename: L S
authors.initials: LS
authors.lastname: Cousens
authors.forename: P
authors.initials: P
authors.lastname: Tekamp-Olson
authors.forename: B A
authors.initials: BA
authors.lastname: Sherry
authors.forename: A
authors.initials: A
authors.lastname: Cerami
mesh_terms: D000818:Animals; D054426:Chemokine CXCL2; D003461:Crystallography; D016207:Cytokines / Q000648:ultrastructure*; D051379:Mice; D015846:Monokines / Q000648:ultrastructure*; D011487:Protein Conformation; D011994:Recombinant Proteins
chemical_list: D054426:Chemokine CXCL2; D016207:Cytokines; D015846:Monokines; D011994:Recombinant Proteins