Tissue-specific variation of pea mitochondrial polypeptides detected by computerized image analysis of two-dimensional electrophoresis gels.
A tissue-specific variation of a total of 152 mitochondria-associated polypeptides is detailed for etiolated leaves, epicotyls and roots from the pea, Pisum satisum L., using computerized image analysis. These organ systems possess, respectively, 128, 100, and 96 mitochondria-associated polypeptides with 38, 8, and 10, respectively, being unique to each. Seventy-one polypeptides were observed to be present in all gels, but some of these also underwent a variety of quantifiable changes. Two polypeptides were identified that varied similarly to a polypeptide of interest (W), known to be abundant in pea epicotyls and potato tubers. It is postulated that these latter three polypeptides are therefore probably related functionally.
Humphrey-Smith
I
I
Département de Microbiologie et Santé Publique, Faculté de Médecine, Brest, France.
Colas des Francs-Small
C
C
Ambart-Bretteville
F
F
Remy
R
R
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005069:Evaluation Studies as Topic; D007887:Fabaceae; D007091:Image Processing, Computer-Assisted; D008928:Mitochondria / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D010946:Plants, Medicinal; D014018:Tissue Distribution
D010455:Peptides; D010940:Plant Proteins