The third molecule associated with interleukin 2 receptor alpha and beta chain.
It is known that the affinity cross-linking study of the human high-affinity Interleukin 2 (IL-2) receptor reveals triplet bands consisting of 70 kDa alpha chain(Tac)-IL-2 and the 90/80 kDa doublet. We found the cell lines lacking the lower band of the doublet in spite of the expression of both alpha and beta chains. No IL-2 binding was detectable in the presence of anti-Tac antibody in these cells. Immunoprecipitation from the cell extract of [125 I] IL-2-cross-linked T cells with anti-beta chain polyclonal IgG detected the upper band, but not lower band of the doublet. These data suggest that the lower band of the doublet represents an unknown IL-2-binding protein (p65) distinct from the beta chain and this molecule may be involved in the intermediate-affinity IL-2 binding together with the beta chain.
Kamio
M
M
First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Arima
N
N
Tsudo
M
M
Imada
K
K
Ohkuma
M
M
Uchiyama
T
T
D016475:3T3 Cells; D000818:Animals; D002461:Cell Line, Transformed; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D007694:Killer Cells, Natural; D007700:Kinetics; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D015375:Receptors, Interleukin-2 / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013388:Succinimides; D013601:T-Lymphocytes; D014162:Transfection
D003432:Cross-Linking Reagents; D007376:Interleukin-2; D046911:Macromolecular Substances; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D013388:Succinimides; C019358:disuccinimidyl suberate