The use of [35S]methionine as a target cell label in long term cytotoxic assays.
Cytotoxic killing assays represent an important experimental tool in immunology to determine specific and/or cross-killing activities of immune effector cells in vitro. To evaluate the killing capacity of effector cells using cytotoxic killing assays, target cells are prelabeled with a radioisotope. Present labeling methods, using 51chromium to prelabel target cells, are associated with some disadvantages. In this study, we tested the use of 35sulfur-methionine ([35S]methionine) to prelabel target cells in long term cytotoxic killing assays. Our results indicate that prelabeling of target cells with [35S]methionine allows the use of a smaller amount of target and effector cells and enables more accurate detection of target cell killing, compared to the presently used labeling methods.
Katz
A
A
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Feldman
M
M
Eisenbach
L
L
D000818:Animals; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D003602:Cytotoxicity, Immunologic; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008715:Methionine; D051379:Mice; D008810:Mice, Inbred C57BL; D013462:Sulfur Radioisotopes; D013997:Time Factors
D002860:Chromium Radioisotopes; D013462:Sulfur Radioisotopes; D008715:Methionine