title: Characterization of the discontinuities in rice tungro bacilliform virus DNA.
abstract: The dsDNA of rice tungro bacilliform virus (RTBV) has two discontinuities, one on each strand, each in a specific position as found in other pararetroviruses. The 5' end of discontinuity 1 was mapped to nucleotide 1 of the published RTBV DNA sequence which suggests that tRNAiMet serves as a primer for negative strand DNA synthesis. This 5' terminus contains up to two ribonucleotides and the 3' terminus overlaps it by five to 25 nucleotides. The discontinuity 2 (D2) did not map to a purine-rich region as has been found in other similar viruses. Both the 5' and 3' termini of D2 were heterogeneous in position giving structures varying from a gap of 10 nucleotides to an overlap of 103 nucleotides.
authors.affiliation: Department of Virus Research, John Innes Institute, Norwich, U.K.
authors.forename: Y
authors.initials: Y
authors.lastname: Bao
authors.forename: R
authors.initials: R
authors.lastname: Hull
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D004267:DNA Viruses / Q000235:genetics*; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D010942:Plant Viruses / Q000235:genetics*; D014771:Virion / Q000235:genetics
chemical_list: D004279:DNA, Viral