Dendritic outgrowth of myenteric plexus neurons in primary culture.
Myenteric plexus neurons derived from neonatal guinea pigs, when exposed to serum, demonstrated a characteristic pattern of growth, including a proliferating outgrowth zone of glial cells, peripheral extension of dendritic processes, and progressive dendritic growth. Serum effects upon dendritic growth, measured morphometrically, was strongly dose- and temporally dependent. Dendritic density was increased 10-fold (120 hr) by the addition of 6% serum, while mean dendritic length was increased 3-fold. Development of cholinergic function was reflected by release of [3H]ACh in response to cholecystokinin octapeptide, vasoactive intestinal peptide, substance P, and calcitonin gene-related peptide (10(-10) and 10(-8) M).
Mulholland
M W
MW
Department of Surgery, University of Michigan, Ann Arbor 48109-0331.
Romanchuk
G
G
Flowe
K
K
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure; D009197:Myenteric Plexus / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D009474:Neurons / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors
D000110:Acetylcholinesterase