[The immunohistochemical study on the recurrent mechanism of acetic acid induced gastric ulcer in rats--extracellular matrix and cellular kinetics].
The localization of fibronectin (FN) and laminin (LM) and cellular kinetics of epithelium in acetic acid induced gastric ulcers were examined by immunohistochemistry, comparing fundic glands ulcer with fundic-pyloric border ulcer. Ulcers on fundic glands had become scars in 30 days and didn't relapse after that. More than half of ulcer on fundic-pyloric border changed into scar in 30 days, however, they started to recur at about 45 days. The localization of LM was observed on basement membrane (BM) beneath epithelia, blood vessel walls and musculature. The percentage change in intra-blood-vessel area compared to total area was analyzed by immunohistochemical distribution of LM. As for ulcer on fundic glands, the percentage of intra-blood-vessel returned to normal numbers after healing to scars (30 days). On the other hand, the percentage of intra-blood-vessel of ulcer on fundic-pyloric border after changing into scar (30 days) and recurring (45 days) significantly increased compared with initial healing (10 days, 20 days). The stratum of spindly cells with FN-positive surface appeared under regenerative epithelia of ulcers on fundic-pyloric border from 10 days to 20 days. This part was proven to be spare in blood vessels by distribution of LM. Though this stratum was not observed at every stages after recurrence, the collagen fibers of ulcer on fundic-pyloric border had got mature thickly. These results may lead the following theory; the phenomenon that the distribution of blood supply in stomach seems to be more dominant in submucosal fibrous layer than in mucosa may cause the recurrence of fundic-pyloric border ulcers. The cell number of S-phase was significantly more numerously observed in relapsed ulcer on fundic-pyloric border than in pre-relapsed one (10 day). The cell number of S-phase on fundic gland ulcer tended to decrease after healing to scar stage. The immediate restoration of connective tissue under the mucosa is considered to promote epithelial cellular kinetics in recovering and maturing.
Yagi
K
K
Third Department of Internal Medicine, Niigata University School of Medicine, Japan.
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D002453:Cell Cycle; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012008:Recurrence; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism*
D000085:Acetates; D005353:Fibronectins; D007797:Laminin; D019342:Acetic Acid