Characterization of monoclonal antibodies against human leukocyte 5-lipoxygenase.
Four mouse monoclonal IgG1 antibody-producing cell lines (5LO-1, 5LO-2, 5LO-3, 5LO-4), produced against highly purified human leukocyte 5-lipoxygenase have been characterized. The monoclonal antibodies produced by these cell lines exhibited differential reactivity against 5-lipoxygenase as determined by ELISA and immunoprecipitation analyses. Monoclonal antibodies 5LO-2 and 5LO-3 inhibited the activity of recombinant human leukocyte 5-lipoxygenase in a dose-dependent manner. This inhibition was selective for 5-lipoxygenase activity since these monoclonal antibodies did not inhibit human leukocyte 15-lipoxygenase or porcine leukocyte 12-lipoxygenase.
Ethier
D
D
Department of Pharmacology, Merck Frosst Centre for Therapeutic Research, Pointe Claire-Dorval, Quebec, Canada.
Evans
J F
JF
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001094:Arachidonate 5-Lipoxygenase / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007962:Leukocytes / Q000201:enzymology; D016859:Lipoxygenase Inhibitors; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D016859:Lipoxygenase Inhibitors; D001094:Arachidonate 5-Lipoxygenase