title: Characteristics and subcellular localization of pristanoyl-CoA synthetase in rat liver.
abstract: We have investigated the activation of pristanic acid to its CoA-ester in rat liver. The results show that peroxisomes, mitochondria as well as microsomes contain pristanoyl-CoA synthetase activity. On the basis of competition experiments and immunoprecipitation studies using antibodies raised against rat liver microsomal long-chain fatty acyl-CoA synthetase (EC 6.2.1.3) we conclude that pristanic acid is activated by the same enzyme which activates long-chain fatty acids, i.e., long-chain fatty acyl-CoA synthetase.
authors.affiliation: Department of Clinical Biochemistry (FO-226), University of Amsterdam, Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Wanders
authors.forename: S
authors.initials: S
authors.lastname: Denis
authors.forename: C W
authors.initials: CW
authors.lastname: van Roermund
authors.forename: C
authors.initials: C
authors.lastname: Jakobs
authors.forename: H J
authors.initials: HJ
authors.lastname: ten Brink
mesh_terms: D000818:Animals; D003066:Coenzyme A Ligases / Q000302:isolation & purification* / Q000378:metabolism; D003998:Dicarboxylic Acids / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D008297:Male; D017830:Octoxynol; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D013347:Subcellular Fractions / Q000201:enzymology; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
chemical_list: D003998:Dicarboxylic Acids; D005227:Fatty Acids; D010169:Palmitic Acids; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D019308:Palmitic Acid; D011092:Polyethylene Glycols; C002844:pristanic acid; D017830:Octoxynol; C036836:dodecanedioic acid; D003066:Coenzyme A Ligases; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase