title: Requirement for three membrane-spanning alpha-helices in the post-translational insertion of a thylakoid membrane protein.
abstract: The insertion of a protein into a lipid bilayer usually involves a short signal sequence and can occur either during or after translation. A light-harvesting chlorophyll a/b-binding protein (LHCP) is synthesized in the cytoplasm of plant cells as a precursor and is post-translationally imported into chloroplasts where it subsequently inserts into the thylakoid membrane. Only mature LHCP is required for insertion into the thylakoid. To define which sequences of the mature protein are necessary and sufficient for thylakoid integration, fusion and deletion proteins and proteins with internal rearrangements were synthesized and incubated with isolated thylakoids and stroma. No evidence is found for the existence of a short signal sequence within LHCP, and, with the exception of the amino terminus and a short lumenal loop, the entire mature protein with consecutively ordered alpha-helices is required for insertion into thylakoid membranes. The addition of positive charges into stromal but not lumenal segments permits the insertion of mutant LHCPs into isolated thylakoids. Replacement of the LHCP transit peptide with the transit peptide from plastocyanin has no effect on LHCP insertion and does not restore insertion of the lumenal charge addition mutants.
authors.affiliation: Department of Botany, Duke University, Durham, North Carolina 27706.
authors.forename: A H
authors.initials: AH
authors.lastname: Auchincloss
authors.forename: A
authors.initials: A
authors.lastname: Alexander
authors.forename: B D
authors.initials: BD
authors.lastname: Kohorn
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007425:Intracellular Membranes / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes; D008051:Lipid Bilayers; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009841:Oligonucleotides / Q000235:genetics; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational
chemical_list: D045342:Light-Harvesting Protein Complexes; D008051:Lipid Bilayers; D008565:Membrane Proteins; D009841:Oligonucleotides; D045322:Photosynthetic Reaction Center Complex Proteins