title: Genetic instability in Streptomyces ambofaciens: inducibility and associated genome plasticity.
abstract: DNA amplification and deletions occur at high frequency in unstable regions localized on the Streptomyces ambofaciens chromosome. The structure of these regions was investigated, leading to the identification of internal reiterations which could play a role in the deletion and/or amplification mechanism(s). UV irradiation and treatments with mitomycin C, oxolinic acid and novobiocin were shown to efficiently induce genetic instability. Finally, mutator strains were isolated, in which genetic instability was dramatically increased. The involvement of an SOS-like response in genetic instability in S. ambofaciens is proposed.
authors.affiliation: Laboratoire de Génétique et Microbiologie, Faculté des Sciences, Université de Nancy I, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Simonet
authors.forename: D
authors.initials: D
authors.lastname: Schneider
authors.forename: J N
authors.initials: JN
authors.lastname: Volff
authors.forename: A
authors.initials: A
authors.lastname: Dary
authors.forename: B
authors.initials: B
authors.lastname: Decaris
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005784:Gene Amplification; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D013045:Species Specificity; D015572:Spiramycin / Q000096:biosynthesis; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D015572:Spiramycin