Whole-cell currents from noncultured human lens epithelium.
Perforated patch techniques were used to measure whole-cell ionic currents in freshly dissociated human lens epithelial cells that had not been subjected to culture media or serum. With a 150 mmol/l K+ internal solution, the cells had resting voltages of -27.4 +/- 4.7 mV (mean +/- standard deviation [SD]) and capacitances of 10.4 +/- 2.8 pF (mean +/- SD). The input resistance of the cells was 1.6 +/- 0.7 G omega (mean +/- SD) at large negative voltages. A delayed outwardly rectifying K+ current was found in most cells studied. Current magnitudes of 1-2 nA at +80 mV were common. The current had selectivities, activation time constants, deactivation time constants, open probability versus voltage relationships, and inactivations similar to those of the delayed rectifying K+ current found in many cell types and studied previously in cultured human lens epithelium. These results verify the existence, at high density, of these currents in noncultured human epithelial cells.
Rae
J L
JL
Department of Physiology, Mayo Foundation, Rochester, Minnesota 55905.
Rae
J S
JS
D000368:Aged; D000369:Aged, 80 and over; D002478:Cells, Cultured; D004594:Electrophysiology; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology*; D008875:Middle Aged; D011188:Potassium / Q000502:physiology; D015221:Potassium Channels / Q000502:physiology; D012964:Sodium / Q000502:physiology
D015221:Potassium Channels; D012964:Sodium; D011188:Potassium