Isolation of a gene encoding a putative leucine zipper structure that is induced by transforming growth factor beta 1 and other growth factors.
A gene sequence (TSC-22) that is induced by transforming growth factor (TGF) beta 1 was isolated by differential screening of a lambda gt10 cDNA library constructed from poly(A)+ RNA of mouse osteoblastic cells treated with TGF-beta 1 for 2 h. TSC-22 gene expression was transcriptionally activated by TGF-beta 1. It was also induced by phorbol 12-myristate 13-acetate, serum, cholera toxin, or dexamethasone, but not appreciably by epidermal growth factor. Its induction was rapid and transient, reaching a peak 2 h after TGF-beta 1 treatment, and was resistant to cycloheximide like that of c-jun. The nucleotide sequences of TSC-22 cDNA showed no homology with any known gene sequence. The open reading frame and in vitro translation product indicated that the gene encodes a polypeptide of 143 amino acids with a molecular mass of 18 kDa that contains a putative leucine zipper structure. Polyclonal antibody was raised against TSC-22 protein expressed in Escherichia coli cells, and the antibody detected a 18-kDa protein in both the cytoplasmic and nuclear fractions of [35S]Met-labeled cells. These results indicate that the TSC-22 gene is a new member of the family of early response genes, and encodes a small polypeptide that is a putative transcriptional regulator.
Shibanuma
M
M
Department of Cancer Cell Research, University of Tokyo, Japan.
Kuroki
T
T
Nose
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001769:Blood; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D006133:Growth Substances / Q000378:metabolism; D016350:Leucine Zippers / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000378:metabolism; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D006133:Growth Substances; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D003907:Dexamethasone; D004247:DNA; D002772:Cholera Toxin; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate