Screening method for beta-lactamase substrate profiles.
A disc diffusion method, based on the idea of Klundert, for screening of substrate profiles of beta-lactamases was developed in order to perform epidemiological studies. The method was tested against 30 different reference beta-lactamases and 59 clinical isolates known to produce TEM-1, SHV-1 and BRO-1. The reproducibility and discriminating power of the disc diffusion method made it possible to differentiate between eight types of substrate profiles for the 30 reference beta-lactamases and to differentiate between TEM-1, SHV-1 and BRO-1 from clinical isolates. In combination with analytical isoelectric focusing the disc diffusion method gives a reliable identification of beta-lactamases.
Schumacher
H
H
Department of Hospital Infections and Antibiotics, Immunological R & D, Copenhagen, Denmark.
Nir
M
M
Mansa
B
B
Thomsen
V F
VF
D000900:Anti-Bacterial Agents / Q000592:standards; D001419:Bacteria / Q000201:enzymology*; D004754:Enterobacter / Q000201:enzymology; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000201:enzymology; D006801:Humans; D007525:Isoelectric Focusing; D007709:Klebsiella / Q000201:enzymology; D007710:Klebsiella Infections / Q000188:drug therapy / Q000201:enzymology; D008722:Methods; D011549:Pseudomonas / Q000201:enzymology; D015203:Reproducibility of Results; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000032:analysis* / Q000145:classification; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases