Biological activity of recombinant interleukin-2 in intravenous admixtures containing antibiotic, morphine sulfate, or total parenteral nutrient solution.
The biological activity of recombinant interleukin-2 in intravenous admixtures containing gentamicin sulfate, tobramycin sulfate, amikacin sulfate, ticarcillin disodium, piperacillin sodium, morphine sulfate, or total parenteral nutrient (TPN) solution was investigated. Recombinant interleukin-2 in a formulation containing human serum albumin was diluted to a therapeutic dose and mixed with the other drugs in test tubes to simulate mixing distal to the Y-site of an i.v. administration set. All test tubes were visually observed for precipitates. Triplicate test samples of recombinant interleukin-2 and interleukin-2 with drug were added to microplate wells containing lymphocyte tissue culture media (an interleukin-2-dependent murine cytotoxic T cell line, CTLL-2) using serial dilutions. The microplates were incubated then pulsed with mouse lymphocyte media containing tritiated thymidine (3H-thymidine). The amount of 3H-thymidine incorporated into lymphocyte DNA was measured using a beta counter. The average counts per minute (cpm) of a sample was analyzed as a function of the logarithm of sample dilution; one interleukin-2 unit was defined as 50% of the maximum cpm measured in the samples. At minimum dilutions, ticarcillin disodium reduced the effect of interleukin-2 on CTLL-2 growth; the other antibiotics and morphine sulfate had no effect. Interleukin-2 mixed with TPN solutions showed slightly enhanced growth of the CTLL-2 line compared with the interleukin-2 standard. Mixtures of multiple antibiotics, morphine, and TPN solutions with and without 20% fat emulsion had no effect on interleukin-2 activity.(ABSTRACT TRUNCATED AT 250 WORDS)
Anderson
P M
PM
Department of Pediatrics, University of Minnesota Hospital and Clinic, Minneapolis 55455.
Rogosheske
J R
JR
Ramsay
N K
NK
Weisdorf
D J
DJ
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D004355:Drug Stability; D004359:Drug Therapy, Combination; D005217:Fat Emulsions, Intravenous / Q000737:chemistry; D006801:Humans; D007376:Interleukin-2 / Q000737:chemistry* / Q000378:metabolism; D009020:Morphine / Q000737:chemistry* / Q000378:metabolism; D010289:Parenteral Nutrition, Total; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
D000900:Anti-Bacterial Agents; D005217:Fat Emulsions, Intravenous; D007376:Interleukin-2; D011994:Recombinant Proteins; D009020:Morphine