Nucleotide sequence of canine c-N-ras: codons 1 to 71.
The c-N-ras gene has been implicated often in the genesis and/or progression of human leukemias. To our knowledge, the sequence of this gene in the dog has not been reported. Using a system of asymmetric reamplification of double-stranded polymerase chain reaction (PCR) products, we have sequenced normal canine c-N-ras mRNA from position -26 to +213, including codons 12, 13, and 61, which are the sites where oncogenic mutations are most commonly observed. The canine c-N-ras sequence has close homology with the human sequence in this area; there were only 6 observed base differences in nucleotide sequence and none resulted in a change of the encoded amino acid. The results of this study set the stage for directed searches for c-N-ras mutations in experimentally induced and naturally acquired neoplasms of the dog.
Saunders
K A
KA
Department of Surgery, School of Veterinary Medicine, University of California, Davis 95616.
Madewell
B R
BR
Oreffo
V I
VI
Kraegel
S A
SA
Gumerlock
P H
PH
D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D003062:Codon / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004285:Dogs / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D003062:Codon; D012333:RNA, Messenger; D004247:DNA