title: DNA methyltransferase is developmentally expressed in replicating and non-replicating male germ cells.
abstract: Genomic methylation patterns are established during maturation of primordial germ cells and during gametogenesis. While methylation is linked to DNA replication in somatic cells, active de novo methylation and demethylation occur in post-replicative spermatocytes during meiotic prophase (1). We have examined differentiating male germ cells for alternative forms of DNA (cytosine-5)-methyltransferase (DNA MTase) and have found a 6.2 kb DNA MTase mRNA that is present in appreciable quantities only in testis; in post-replicative pachytene spermatocytes it is the predominant form of DNA MTase mRNA. The 5.2 kb DNA MTase mRNA, characteristic of all somatic cells, was detected in isolated type A and B spermatogonia and haploid round spermatids. Immunobolt analysis detected a protein in spermatogenic cells with a relative mass of 180,000-200,000, which is close to the known size of the somatic form of mammalian DNA MTase. The demonstration of the differential developmental expression of DNA MTase in male germ cells argues for a role for testicular DNA methylation events, not only during replication in premeiotic cells, but also during meiotic prophase and postmeiotic development.
authors.affiliation: McGill University, Montreal Children's Hospital Research Institute, Quebec, Canada.
authors.forename: J M
authors.initials: JM
authors.lastname: Trasler
authors.forename: A A
authors.initials: AA
authors.lastname: Alcivar
authors.forename: L E
authors.initials: LE
authors.lastname: Hake
authors.forename: T
authors.initials: T
authors.lastname: Bestor
authors.forename: N B
authors.initials: NB
authors.lastname: Hecht
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics; D015257:DNA-Cytosine Methylases / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D008297:Male; D008745:Methylation; D051379:Mice; D008815:Mice, Inbred Strains; D013090:Spermatocytes / Q000166:cytology / Q000201:enzymology*; D013091:Spermatogenesis; D014407:Tumor Cells, Cultured
chemical_list: D015257:DNA-Cytosine Methylases