Characterization of rat liver mannan-binding protein gene.
We previously found that rat liver mannan-binding protein (L-MBP) is encoded by two species of mRNA of 1.4 and 3.5 kb long. In this study, the structure of the gene encoding rat L-MBP was determined from the sequences of isolated genomic DNA clones and PCR amplified DNA fragments. Rat L-MBP is encoded by at least three species of mRNA, the differences among which are generated by an alternative splicing at the 5'-nontranslated region and an alternative utilization of polyadenylation sites. The rat L-MBP gene consists of six exons separated by five introns. The coding region of rat L-MBP mRNA is encoded by four exons (Exons III-VI), the 5'-noncoding region by Exons I and II, and the 3'-noncoding region by Exon VI. The exon-intron boundaries of L-MBP are completely identical to those of rat serum and human MBP, suggesting that all three MBPs are derived from a common ancestral gene.
Wada
M
M
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University.
Itoh
N
N
Ohta
M
M
Kawasaki
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics*; D037222:Collectins; D004247:DNA / Q000235:genetics; D005091:Exons; D015698:Genomic Library; D007438:Introns; D008099:Liver / Q000737:chemistry; D008351:Mannans / Q000378:metabolism*; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping
D002352:Carrier Proteins; D037222:Collectins; D008351:Mannans; D012333:RNA, Messenger; D004247:DNA