title: Dodeca satellite: a conserved G+C-rich satellite from the centromeric heterochromatin of Drosophila melanogaster.
abstract: To identify sequences from the centromeric region, we have constructed a Drosophila melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA from the minichromosome Dp(1;f)1187 derived from the X chromosome. We describe the structure of one clone isolated in this way. This YAC is structurally unstable and contains tandemly repeated G+C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences are found in the genomes of organisms as distant as Arabidopsis thaliana and Homo sapiens.
authors.affiliation: Centro de Biología Molecular (CSIC-UAM), Spain.
authors.forename: J P
authors.initials: JP
authors.lastname: Abad
authors.forename: M
authors.initials: M
authors.lastname: Carmena
authors.forename: S
authors.initials: S
authors.lastname: Baars
authors.forename: R D
authors.initials: RD
authors.lastname: Saunders
authors.forename: D M
authors.initials: DM
authors.lastname: Glover
authors.forename: P
authors.initials: P
authors.lastname: Ludeña
authors.forename: C
authors.initials: C
authors.lastname: Sentis
authors.forename: C
authors.initials: C
authors.lastname: Tyler-Smith
authors.forename: A
authors.initials: A
authors.lastname: Villasante
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002503:Centromere / Q000737:chemistry*; D015825:Chromosomes, Fungal; D003596:Cytosine; D004276:DNA, Satellite / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D015723:Gene Library; D016678:Genome; D006147:Guanine; D006570:Heterochromatin / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome
chemical_list: D004276:DNA, Satellite; D006570:Heterochromatin; D006147:Guanine; D003596:Cytosine