The identification of a tandem H-DNA structure in the c-myc nuclease sensitive promoter element.
Previous studies have shown that the c-myc nuclease sensitive element (NSE) is capable of forming H-DNA in vitro. The NSE sequence exhibits strong purine/pyrimidine strand asymmetry. To study the NSE further, we have isolated the element from other c-myc sequences and have shown that the NSE alone is sufficient for the formation of H-DNA in supercoiled plasmids. We also show that the NSE forms a complex structure containing both H-y3 and H-y5 H-DNA. We term this structure tandem H-DNA.
Firulli
A B
AB
Department of Molecular Diagnostics, Roswell Park Cancer Institute, Buffalo, NY 14263.
Maibenco
D C
DC
Kinniburgh
A J
AJ
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003851:Deoxyribonucleases / Q000494:pharmacology; D016259:Genes, myc / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic / Q000235:genetics*
D004247:DNA; D003851:Deoxyribonucleases