Sequence similarity between macrolide-resistance determinants and ATP-binding transport proteins.
The three macrolide-resistance-encoding genes, tlrC from Streptomyces fradiae, srmB from Streptomyces ambofaciens, and carA from Streptomyces thermotolerans, encode proteins that possess significant sequence similarity to ATP-dependent transport proteins. The N-terminal and C-terminal halves of these proteins are very similar to each other and contain highly conserved regions that resemble ATP-binding domains typically present within the superfamily of ATP-dependent transport proteins. These observations suggest that the mechanism by which these genes confer resistance to macrolides is due to export of the antibiotics, a process that is driven by energy derived from ATP hydrolysis.
Schoner
B
B
Lilly Research Laboratories, Division of Eli Lilly and Company, Indianapolis, IN 46285.
Geistlich
M
M
Rosteck
P
P
Rao
R N
RN
Seno
E
E
Reynolds
P
P
Cox
K
K
Burgett
S
S
Hershberger
C
C
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry; D004352:Drug Resistance, Microbial / Q000235:genetics; D018942:Macrolides; D008969:Molecular Sequence Data; D011485:Protein Binding; D016415:Sequence Alignment; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D018942:Macrolides; D000255:Adenosine Triphosphate