title: 20-Hydroxyeicosatetraenoic acid is excreted as a glucuronide conjugate in human urine.
abstract: 20-hydroxyeicosatetraenoic acid, a major renal P-450 metabolite of arachidonic acid, has been quantified in human urine using capillary gas chromatography/electron capture negative ion chemical ionization mass spectrometry. The urinary excretion of 20-hydroxyeicosatetraenoic acid was in the low pg/ml range. However, treatment of urine with beta-glucuronidase resulted in a 13- to 28-fold increase in its concentration. This suggests 20-hydroxyeicosatetraenoic acid differs from other eicosanoids in that it is excreted primarily as a glucuronide conjugate.
authors.affiliation: Department of Pharmacology, Vanderbilt University, Nashville, TN 37232.
authors.forename: C
authors.initials: C
authors.lastname: Prakash
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhang
authors.forename: J R
authors.initials: JR
authors.lastname: Falck
authors.forename: K
authors.initials: K
authors.lastname: Chauhan
authors.forename: I A
authors.initials: IA
authors.lastname: Blair
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005965:Glucuronates / Q000652:urine*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000652:urine*
chemical_list: D005965:Glucuronates; D006893:Hydroxyeicosatetraenoic Acids; C055987:20-hydroxy-5,8,11,14-eicosatetraenoic acid