title: Reaction mechanism of protocatechuate 3,4-dioxygenase.
abstract: We have observed that high SOD-like function (decomposition of superoxide anion) was observed for several iron(III) compounds with tripodal ligands and several oxovanadium(IV) compounds, and also that these compounds exhibit high catalytic activity for oxidative cleavage of 3,5-di-tert-butylcatechol in non-donating solvents such as dichloromethane or nitromethane. These are suggesting that the same reaction intermediate exists in reaction mixtures of both the SOD-like and catecholase-like functions of these compounds. Based on these facts, we have proposed a new reaction mechanism for the oxidative cleavage of catechol catalyzed by the native non-heme iron dioxygenases; this includes formation of an iron(III)-peroxide adduct as a reaction intermediate, which exhibits electrophilic nature.
authors.affiliation: Department of Chemistry, Faculty of Science, Yamagata University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nishida
authors.forename: K
authors.initials: K
authors.lastname: Yoshizawa
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: I
authors.initials: I
authors.lastname: Watanabe
mesh_terms: D001665:Binding Sites; D008954:Models, Biological; D011520:Protocatechuate-3,4-Dioxygenase / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D011520:Protocatechuate-3,4-Dioxygenase; D013482:Superoxide Dismutase