Evidence for translation of apple stem grooving capillovirus genomic RNA.
Apple stem grooving virus (ASGV) RNA was translated in a rabbit reticulocyte lysate system and shown to direct the synthesis of several polypeptides of Mr ranging from 200K to 43K. A polypeptide of 200K was a major product, but no polypeptide with electrophoretic mobility the same as that of the ASGV coat protein was synthesized. Immunoprecipitation experiments showed that a polypeptide of 200K was selectively precipitated by antiserum against purified ASGV. These results indicate that ASGV coat protein is translated as part of a 200K polyprotein.
Yoshikawa
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Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka, Japan.
Takahashi
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T
D002474:Cell-Free System / Q000378:metabolism; D005638:Fruit / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D010942:Plant Viruses / Q000235:genetics*; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000378:metabolism*; D012156:Reticulocytes / Q000378:metabolism; D014197:Trees / Q000382:microbiology; D014777:Virus Diseases / Q000175:diagnosis
D012367:RNA, Viral