title: Bradykinin stimulates the production of prostaglandin E2 and interleukin-6 in human osteoblast-like cells.
abstract: The effect of bradykinin (BK) on proteinase activity, prostaglandin synthesis, and the production of interleukin-6 (IL-6) was investigated in cultures of human osteoblast-like cells. Bradykinin had no effect on stromelysin activity and plasminogen activator activity produced by human osteoblast-like cells. However, BK stimulated the production of prostaglandin E2, an effect that was markedly enhanced by pre-incubation with recombinant interleukin-1 alpha (rhIL-1 alpha), but was apparently unaffected by BK receptor antagonists types 1 and 2. Bradykinin stimulated the intracellular accumulation of total inositol phosphates suggesting that its effects were mediated by stimulation of phosphoinositide metabolism. Bradykinin within the dose range of 10(-11)-10(-5) M also significantly stimulated the production of IL-6. Bradykinin may, therefore, mediate a variety of responses in bone under both physiological and pathological conditions.
authors.affiliation: Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, UK.
authors.forename: S
authors.initials: S
authors.lastname: Rahman
authors.forename: R A
authors.initials: RA
authors.lastname: Bunning
authors.forename: P R
authors.initials: PR
authors.lastname: Dobson
authors.forename: D B
authors.initials: DB
authors.lastname: Evans
authors.forename: K
authors.initials: K
authors.lastname: Chapman
authors.forename: T H
authors.initials: TH
authors.lastname: Jones
authors.forename: B L
authors.initials: BL
authors.lastname: Brown
authors.forename: R G
authors.initials: RG
authors.lastname: Russell
mesh_terms: D001920:Bradykinin / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis*; D007700:Kinetics; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D007295:Inositol Phosphates; D015850:Interleukin-6; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D015232:Dinoprostone; D001920:Bradykinin