Limited size of the fragile X site shown by fluorescence in situ hybridization.
Cosmids, isolated from a 475 kb YAC that spans the fragile X region, and the YAC itself, were used for fluorescence in situ hybridization (FISH) on metaphase chromosomes from fragile X patients. Cosmid 22.3, containing most of the hybrid translocation breakpoints, shows in situ hybridization signals distal and proximal from the fragile X site. We propose that the size of the fragile site is limited to 20 kb.
Verkerk
A J
AJ
Department of Clinical Genetics, University Hospital, Dijkzigt, The Netherlands.
Eussen
B H
BH
Van Hemel
J O
JO
Oostra
B A
BA
D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002874:Chromosome Mapping; D003360:Cosmids; D004271:DNA, Fungal / Q000235:genetics; D005453:Fluorescence; D005600:Fragile X Syndrome / Q000235:genetics*; D005821:Genetic Techniques; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D014960:X Chromosome
D004271:DNA, Fungal