title: Development of a non-invasive transcutaneous blood glucose monitoring method using an ISFET biosensor.
abstract: A transcutaneous blood glucose monitoring method has been developed by combining an SOS/ISFET (silicone-on-sapphire/ion sensitive field effect transistor) biosensor with an SEF (suction effusion fluid) collecting technique. The SEF collecting cell has a stainless steel mesh at its bottom which is kept in a weak vacuum condition so as to suck up transcutaneous SEF and deposit it in a reservoir above. An ISFET biosensor, consisting of immobilized enzyme membranes, enables the detection of glucose concentrations in very small samples. The method has been successfully applied to non-invasive monitoring of human blood glucose levels during tests involving 75 g OGTT (oral glucose tolerance test). Glucose concentrations measured with SEF showed good correlation with those obtained directly from serum. Results suggest the feasibility of a portable blood monitoring system.
authors.affiliation: Central Research Laboratories, NEC Corporation, Kanagawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ito
authors.forename: A
authors.initials: A
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Kuriyama
authors.forename: J
authors.initials: J
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Arai
authors.forename: M
authors.initials: M
authors.lastname: Kikuchi
authors.forename: S
authors.initials: S
authors.lastname: Kayashima
authors.forename: N
authors.initials: N
authors.lastname: Nagata
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D015190:Blood Glucose Self-Monitoring / Q000379:methods*; D005951:Glucose Tolerance Test; D006801:Humans
chemical_list: D001786:Blood Glucose