title: Subcellular localisation of guinea pig hepatic molybdenum hydroxylases.
abstract: Molybdenum hydroxylase activity in guinea pig liver has been compared with that of marker enzymes in mitochondria (succinate dehydrogenase), microsomes (glucose-6-phosphatase) and cytosol (lactate dehydrogenase). Aldehyde oxidase activity was highest in the cytosol, with about 10-fold activity of xanthine oxidase. Significant molybdenum hydroxylase activity was found in mitochondria with minimal levels in microsomes. Mitochondrial and cytosolic aldehyde oxidase varied in substrate specificity and electrophoretic mobility with two major bands in each fraction, one of which was common to cytosol and mitochondria.
authors.affiliation: University of Bradford, West Yorkshire, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Critchley
authors.forename: D J
authors.initials: DJ
authors.lastname: Rance
authors.forename: C
authors.initials: C
authors.lastname: Beedham
mesh_terms: D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000032:analysis*; D000818:Animals; D015415:Biomarkers; D006168:Guinea Pigs; D007527:Isoenzymes; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000032:analysis*; D008982:Molybdenum / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D014969:Xanthine Oxidase
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D008982:Molybdenum; D006899:Mixed Function Oxygenases; D014969:Xanthine Oxidase; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase