Activation and quantitative estimation of bacteriophage T4 late regulatory signal in cis- and transconditions.
Activation of the bacteriophage T4 late gene 22 carried on the phage genome (ciscondition) or borne on a plasmid (transcondition) was quantitatively analyzed after phage infection using a lacZ reporter gene. Transcription from the late promoter was activated postreplicatively by concomitantly infecting T4 phage. A truncated T4-lacZ gene including only 15 bp of sequence upstream of the late promoter consensus of gene 22 TATAAATA, was fully activated in the ciscondition but induced to a reduced extent in the transcondition. A possible mechanism for trans-activation is discussed.
Noguchi
T
T
Research Laboratories, Yamasa Shoyu Co. Ltd., Chiba, Japan.
Takahashi
H
H
D004274:DNA, Recombinant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D010957:Plasmids; D015183:Restriction Mapping; D013604:T-Phages / Q000235:genetics*; D015533:Transcriptional Activation; D001616:beta-Galactosidase / Q000378:metabolism
D004274:DNA, Recombinant; D001616:beta-Galactosidase