Polyacrylamide gel electrophoresis analysis of ribosomal protein: a new approach for actinomycete taxonomy.
The ribosomal (r)-proteins from eleven Streptomyces strains representing various numerical taxonomic clusters were compared by two-dimensional polyacrylamide-gel electrophoresis (2D-PAGE). The protein patterns were specific for each species. An attempt was made to identify one strain of Streptomyces by both traditional taxonomic methods and 2D-PAGE analysis of the r-protein patterns. Both methods identified the strain as Streptomyces lavendulae, and protein pattern analysis also showed that S. griseolavendus was a variant of S. lavendulae. Actinomycete r-protein AT-L30 exhibited electrophoretic mobility that is specific for each genus. On the basis of this observation, we analyzed AT-L30 r-proteins from numerous strains of species belonging to the genera Actinomadura, Microtetraspora, Streptosporangium, Nocardia, Rhodococcus and mycolate-less wall chemotype-IV actinomycetes. The results strongly supported the conclusions of previous work and thus proved the efficacy of r-protein analysis as a novel approach for taxonomy of actinomycetes.
Ochi
K
K
National Food Research Institute, Ibaraki, Japan.
D000192:Actinomycetales / Q000145:classification*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D012269:Ribosomal Proteins / Q000737:chemistry*
D012269:Ribosomal Proteins