title: Extracellular matrix synthesis and turnover by type II pulmonary epithelial cells.
abstract: Both type I and type II pulmonary epithelial cells contact the extracellular matrix (ECM). Type II cell-ECM interactions are bidirectional; they involve matrix-mediated modulation of type II cell differentiation, as well as cellular synthesis and deposition of ECM components. The present experiments examine the kinetics of accumulation of newly synthesized proteins in cell and matrix fractions from primary cultures of type II pneumocytes. Cycloheximide-sensitive incorporation of [3H]leucine into total protein of both the cell and ECM fractions was linear for 24-30 h, when steady-state labeling was reached and maintained to at least day 8. Over this interval, the cells enlarged but did not divide. Newly synthesized proteins recovered in the matrix fraction averaged 1-2% of those in the cells. Relative rates of radiolabeling of matrix proteins peaked at culture day 2 and increased in the absence of serum. In short-pulse studies, initial rates of protein synthesis were equal on culture days 1 and 3; this suggested that the steady-state labeling kinetics above reflected protein turnover. This was supported by rapid loss of radioactivity from the ECM after fresh type II cells were seeded on a prelabeled, cell-free matrix surface. Fresh or conditioned Dulbecco's modified Eagle's medium containing 10% fetal calf serum had little effect on matrix stability. These results demonstrate regulated deposition and turnover of a complex ECM by type II cells and provide a basis for further investigations of factors that control these processes.
authors.affiliation: Department of Cellular and Molecular Physiology, College of Medicine, Pennsylvania State University, Hershey 17033.
authors.forename: D E
authors.initials: DE
authors.lastname: Rannels
authors.forename: S E
authors.initials: SE
authors.lastname: Dunsmore
authors.forename: R N
authors.initials: RN
authors.lastname: Grove
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors
chemical_list: D016326:Extracellular Matrix Proteins; D007930:Leucine