[Electrophoresis study of hemocyanin of the polytypic species Idotea chelipes and Idotea balthica basteri (Crustacea Isopoda)].
Hemocyanin has been used as a protein marker for Crustacea speciation. The specificity of the hemocyanin fractions of two Idotea species has been confirmed by mono and bidirection electrophoresis. Four protein fractions have been found in the two Idotea species. However, the molecular weight of each Idotea chelipes fraction is slightly higher. The two fractions of high molecular weight are strongly associated. We assumed a molecular weight of 240,000 for I. chelipes and 230,000 for I. balthica basteri. The two other protein fractions are well separated and have a weight of 130,000 and 77,000 in I. chelipes; 120,000 and 75,000 for I. b. basteri. In contrast, the subspeciation of I. chelipes has not been clearly demonstrated by electrophoresis of the hemocyanin.
Charfi-Cheikhrouha
F
F
Laboratoire de Biologie et de Biochimie, Faculté des Sciences de Tunis, Campus Universitaire, Tunisie.
Belhadj
O
O
D000818:Animals; D003445:Crustacea / Q000737:chemistry* / Q000145:classification; D004586:Electrophoresis; D015180:Electrophoresis, Gel, Two-Dimensional; D006433:Hemocyanins / Q000737:chemistry*; D008970:Molecular Weight
D006433:Hemocyanins
Etude électrophorétique de l'hémocyanine de l'espèce polytypique Idotea chelipes et d'Idotea balthica basteri (Crustacés Isopodes).