title: Evaluation of Salmonella typhimurium strains harbouring defined mutations in htrA and aroA in the murine salmonellosis model.
abstract: Derivatives of the mouse-virulent Salmonella typhimurium strain SL1344 were constructed harbouring defined mutations in htrA, aroA or htrA aroA combined. When administered orally or intravenously to BALB/c mice, all the mutants were found to be highly attenuated. All mutants were able to confer significant protection against lethal challenge with SL1344 after a single oral dose of live organisms. SL1344 htrA mutants persisted in livers and spleens at a lower level than SL1344 aroA mutants after intravenous administration. SL1344 htrA aroA mutants persisted at an even lower level and were cleared from the livers and spleens of mice within 21 days of intravenous administration. Thus htrA and htrA aroA mutants can be considered as potential oral vaccines against salmonellosis.
authors.affiliation: Vaccines Research Unit, Medeva Group Research, Wellcome Research Labs, Beckenham, Kent, U.K.
authors.forename: S N
authors.initials: SN
authors.lastname: Chatfield
authors.forename: K
authors.initials: K
authors.lastname: Strahan
authors.forename: D
authors.initials: D
authors.lastname: Pickard
authors.forename: I G
authors.initials: IG
authors.lastname: Charles
authors.forename: C E
authors.initials: CE
authors.lastname: Hormaeche
authors.forename: G
authors.initials: G
authors.lastname: Dougan
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D015183:Restriction Mapping; D012481:Salmonella Infections, Animal / Q000382:microbiology* / Q000517:prevention & control; D012486:Salmonella typhimurium / Q000235:genetics* / Q000472:pathogenicity; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014774:Virulence / Q000235:genetics
chemical_list: D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; D014613:Vaccines, Attenuated