[Genetic recombination and Fib genes transfer of the plasmid of E. herbicola CSH 1065].
We inserted kanamycin resistance (Km) gene and mobilized function (Mob) gene on the plasmid of E. herbicola CSH1065 by using DNA molecular cloning and genetic recombination techniques. Therefore, the plasmid of E. herbicola CSH1065 to E. coli HB101 could be transferred by conjugation. The expression of those genes concerning fungi inhibition function (Fib) of E. herbicola CSH1065 in E. coli HB101 was observed. This result confirmed again the fungi inhibition genes of E. herbicola CSH1065 is only related to its plasmid genome not to its chromosome genome. Meanwhile those yellow pigment genes located on the plasmid don't involve the antifungi function of E. herbicola CSH1065. All those results were convinced by DNA molecular hybridizations.
Zhao
Y
Y
Kunming Institute of Botany, Academy of Science of China.
Tenning
P
P
D004925:Escherichia / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic; D014162:Transfection