title: Effect of Bacillus Calmette-Guérin inoculation on numbers of dendritic cells in bronchoalveolar lavages of rats.
abstract: Dendritic cells (DC) are present in all lymphoid tissues and are widely distributed in the airway epithelium and lung parenchyma. In this study DC were morphologically and cytochemically identified in normal rat bronchoalveolar lavage (BAL), although in very low percentages. Furthermore, the total population as well as different Percoll density fractions demonstrated poor antigen-presenting capacity and even suppressed antigen-specific stimulation by rat splenic DC. In contrast, when an inflammatory response was induced by intratracheal inoculation of Bacillus Calmette-Guérin (BCG), an increase of Ia-positive cells, containing high percentages of monocytes and DC (MDC) was found. In BAL, DC increased about 25 times within 48 h after BCG inoculation. These BCG-induced BAL cells as well as the different density fractions showed a high antigen-presenting capacity at low concentrations. However, at higher concentrations they were suppressive, except for the highest density fraction which lacked alveolar macrophages (AM). These results indicate that the increased numbers of Ia-positive MDC during an inflammatory reaction are very likely responsible for antigen presentation in vitro. In contrast, AM suppress the antigen-specific T cell proliferation in a concentration dependent manner.
authors.affiliation: Department of Cell Biology, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: C E
authors.initials: CE
authors.lastname: Havenith
authors.forename: A J
authors.initials: AJ
authors.lastname: Breedijk
authors.forename: E C
authors.initials: EC
authors.lastname: Hoefsmit
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology*; D002452:Cell Count; D003713:Dendritic Cells / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation; D016676:Macrophages, Alveolar / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology*; D051381:Rats; D011912:Rats, Inbred ACI; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology; D014132:Trachea / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal