title: Potential of a recombinant antigen as a prophylactic vaccine for day-old broiler chickens against Eimeria acervulina and Eimeria tenella infections.
abstract: A genetically engineered Eimeria tenella antigen (GX3262), produced as a fusion protein with beta-galactosidase and identified with a monoclonal antibody, induced partial but significant protection in young broiler chickens against experimental E. tenella and Eimeria acervulina infections. The antigen appears to share a T-helper cell epitope with the parasite as evidenced by (a) booster inoculation with either the recombinant antigen or with a small number of live oocysts enhanced the protective immunity in GX3262 primed chickens, and (b) ability of the antigen to induce in vitro stimulation of T-cells from chickens immunized with antigen or parasite. These observations suggest the feasibility of a single vaccination of 1 or 2-day-old broilers with GX3262 to induce an acceptable degree of protective immunity. The implications of the observations reported here are far reaching in terms of a practical coccidiosis vaccine for poultry, and show for the first time that 1-day-old broiler chickens can be efficiently vaccinated with a recombinant antigen against one or more species of Eimeria.
authors.affiliation: Department of Molecular Biology, A.H. Robins Research Laboratories, Richmond, VA 23220.
authors.forename: B S
authors.initials: BS
authors.lastname: Bhogal
authors.forename: G A
authors.initials: GA
authors.lastname: Miller
authors.forename: A C
authors.initials: AC
authors.lastname: Anderson
authors.forename: E J
authors.initials: EJ
authors.lastname: Jessee
authors.forename: S
authors.initials: S
authors.lastname: Strausberg
authors.forename: R
authors.initials: R
authors.lastname: McCandliss
authors.forename: J
authors.initials: J
authors.lastname: Nagle
authors.forename: R L
authors.initials: RL
authors.lastname: Strausberg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology / Q000662:veterinary*; D004539:Eimeria / Q000235:genetics / Q000276:immunology*; D016786:Eimeria tenella / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D007109:Immunity; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D014614:Vaccines, Synthetic