Characterization of the promoter region of the basidiomycete Lentinus edodes Le.ras gene.
An analysis of the 2.7-kb nucleotide sequence including the 5'-flanking region of the basidiomycete Lentinus edodes Le.ras-encoding gene revealed that the promoter region contains a CAAT box, a CACCC box, two consecutive TATAAA boxes, and a CT-rich sequence element, in that order, from upstream to downstream. One major and two alternative transcriptional initiation sites are located 132 nucleotides and 85 and 90 nucleotides downstream from the downstream TATAAA box, and the major site is positioned just in the CT-rich sequence.
Kajiwara
S
S
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
Shishido
K
K
D000363:Agaricales / Q000235:genetics*; D001483:Base Sequence; D011905:Genes, ras / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic