Absence of 2-phenylethylamine binding after monoamine oxidase inhibition in rat brain.
Earlier work has suggested the existence of saturable and highly specific binding sites for [3H]2-phenylethylamine in rat forebrain membranes. Since monoamine oxidase (MAO) was not inhibited during the assay, the [3H]2-phenylethylamine binding may have been affected by an interaction between 2-phenylethylamine and the enzyme. This is an investigation of [3H]2-phenylethylamine binding to rat forebrain membranes in the presence of two MAO inhibitors, (-)-deprenyl and pargyline. The results show that the high affinity specific binding of [3H]2-phenylethylamine to rat forebrain membranes is inhibited by pretreatment of the membrane with the MAO inhibitors and in vivo injection of the MAO inhibitors in a concentration-dependent manner. In the presence of higher concentrations of MAO inhibitors, the specific binding of [3H]2-phenylethylamine is completely blocked, suggesting that the binding sites reported earlier represent binding to MAO-B.
Li
X M
XM
Neuropsychiatric Research Unit, University of Saskatchewan, Saskatoon, Canada.
Juorio
A V
AV
Paterson
I A
IA
Boulton
A A
AA
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects; D010293:Pargyline / Q000494:pharmacology; D010627:Phenethylamines / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D012642:Selegiline / Q000494:pharmacology
D008996:Monoamine Oxidase Inhibitors; D010627:Phenethylamines; D012642:Selegiline; C029261:phenethylamine; D010293:Pargyline