Characterization of two human monoclonal IgM antibodies that recognize nuclear lamins.
Using immunofluorescence and immunoblotting techniques, we have identified monoclonal IgM lambda from two patients that are specific for lamins A and C and lamin B, respectively. Lamins A, B, and C are peripheral membrane proteins of the nuclear envelope with structural similarities to cytoplasmic intermediate filament proteins. When studied by indirect immunofluorescence on rat tissues, the serum containing anti-lamin B IgM stained smooth and striated muscles in addition to nuclear envelopes. Lamin B antibodies affinity purified from this serum were able to label muscle cells, suggesting that lamin B shares an epitope(s) with an unidentified muscular component(s). Since in an enzyme-linked immunosorbent assay there was no reactivity with a panel of proteins which are frequent targets of "natural" antibodies, these monoclonal IgM appear to belong to the rare category of IgM that possess a restricted specificity.
Lassoued
K
K
Laboratoire d'Immunochimie et Immunopathologie, U108 INSERM, Hôpital Saint-Louis, Paris.
André
C
C
Danon
F
F
Modigliani
R
R
Dhumeaux
D
D
Clauvel
J P
JP
Brouet
J C
JC
Courvalin
J C
JC
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007075:Immunoglobulin M / Q000276:immunology* / Q000302:isolation & purification; D007146:Immunoglobulin lambda-Chains / Q000276:immunology; D034921:Lamin Type B; D034882:Lamins; D009132:Muscles / Q000276:immunology; D009685:Nuclear Envelope / Q000276:immunology*; D009687:Nuclear Proteins / Q000276:immunology*; D051381:Rats
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D007146:Immunoglobulin lambda-Chains; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins