Ethanol stimulates immunoreactive endothelin-1 and -2 release from cultured human umbilical vein endothelial cells.
The present study employed enzyme-immunoassay to examine the effect of ethanol on endothelin-1 and/or -2(ET1 + 2) release from human umbilical vein endothelial cells. Thirty minutes of exposure to ethanol increased the release of immunoreactive ET1 + 2 from cultured endothelial cells in a dose-dependent manner. However, ethanol at concentrations of less than 400 mM did not induce any LDH release from the endothelial cells. Trypan blue exclusion test revealed that 400 mM solution of ethanol decreased the cell viability to 7.7%. Thus, ethanol was found to directly stimulate ET1 + 2 release from cultured human umbilical vein endothelial cells. This reaction of vascular endothelial cells against ethanol may be related to ethanol-induced cardiovascular diseases such as hypertension, myocardial infarction and stroke, as well as fatal alcohol syndrome.
Tsuji
S
S
Department of Medicine, Osaka University Medical School, Japan.
Kawano
S
S
Michida
T
T
Masuda
E
E
Nagano
K
K
Takei
Y
Y
Fusamoto
H
H
Kamada
T
T
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism
D016232:Endothelins; D000431:Ethanol; D007770:L-Lactate Dehydrogenase