Lymphoid development in mice congenitally lacking T cell receptor alpha beta-expressing cells.
Vertebrate T cells express either an alpha beta or gamma delta T cell receptor (TCR). The developmental relatedness of the two cell types is unresolved. alpha beta + T cells respond to specific pathogens by collaborating with immunoglobulin-producing B cells in distinct lymphoid organs such as the spleen and Peyer's patches. The precise influence of alpha beta + T cells on B cell development is poorly understood. To investigate the developmental effects of alpha beta + T cells on B cells and gamma delta + T cells, mice homozygous for a disrupted TCR alpha gene were generated. The homozygotes showed elimination of alpha beta + T cells and the loss of thymic medullae. Despite this, gamma delta + T cells developed in normal numbers, and there was an increase in splenic B cells.
Philpott
K L
KL
Imperial Cancer Research Fund, London, United Kingdom.
Viney
J L
JL
Kay
G
G
Rastan
S
S
Gardiner
E M
EM
Chae
S
S
Hayday
A C
AC
Owen
M J
MJ
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001755:Blastocyst; D015139:Blotting, Southern; D002678:Chimera; D002999:Clone Cells; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D008221:Lymphoid Tissue / Q000254:growth & development / Q000276:immunology*; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains; D010581:Peyer's Patches / Q000276:immunology; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D004247:DNA