The effects of acute ethanol on growth in rat liver: steady state c-myc transcripts.
In order to elucidate the effects of acute ethanol on compensatory liver growth (regeneration), the steady state c-myc mRNA levels were studied following two-thirds partial hepatectomy. After surgery, control rat livers exhibited two peaks of c-myc transcripts, at 0.5-2 h and at 8-10 h. Sham surgery did not induce c-myc mRNA expression. Ethanol (3 g/kg), administered by gavage at 1 hour prehepatectomy, had no effect on the initial peak of c-myc mRNA; however, the second peak was eliminated. Control gavage of isocaloric glucose prior to partial hepatectomy had no effects on either of the subsequent c-myc mRNA peaks. Blood alcohol levels were found to be elevated throughout the prereplicative phase. These results suggest that ethanol may disrupt proto-oncogene expression near the restriction point at the G1/S boundary of the cell cycle in hepatocytes.
Lumpkin
C K
CK
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock 72205.
Taylor
J M
JM
Tarpley
M D
MD
Hayden
J B
JB
Badger
T M
TM
McClung
J K
JK
D000818:Animals; D015152:Blotting, Northern; D000431:Ethanol / Q000097:blood / Q000494:pharmacology*; D006498:Hepatectomy / Q000379:methods; D006706:Homeostasis; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014158:Transcription, Genetic
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D000431:Ethanol