Activities of pork muscle proteases in model cured meat systems.
The effect of curing agents (salt, nitrate, ascorbic acid and glucose) and processing parameters (pH, water activity and drying and cooking temperatures) on pork muscle cathepsins B, D, H and L as well as leucyl, arginyl and tyrosyl hydrolysing activities is reported. Salt (60 g/l) showed a powerful inhibitory effect, especially on cathepsin D and aminopeptidase activities where less than 13% of the original activity was recovered. Cathepsin H was also affected (38% of the original activity) while cathepsins B and B+L recovered 72.5 and 63.0%, respectively. Nitrate (0.2-0.25 g/l) and ascorbic acid (0.2-0.4 g/l) did not significantly affect the enzyme activities. On the other hand, 0.5-2 g/l of glucose activated both cathepsins B and D with an increase of 39.5 and 28.5% and also leucyl and arginyl hydrolysing activities which were 75.0 and 24.0%, respectively. No aminopeptidase activity was detected when assayed in 100 mM sodium citrate buffer, pH 5.1. Cathepsin H was also very affected at that pH and only 12.0% of activity was recovered. A decrease in water activity, especially below 0.84, also affected the enzyme activities which were found below 50%. Temperatures in the usual range of the drying process (22 and 30 degrees C) gave substantial enzyme activities (around 40-50 and 80%, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
Toldrá
F
F
Instituto de Agroquímica y Tecnología de Alimentos (CSIC) Valencia, Spain.
Rico
E
E
Flores
J
J
D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002403:Cathepsins / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005285:Fermentation; D005947:Glucose / Q000494:pharmacology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008461:Meat Products; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D009566:Nitrates / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013552:Swine; D014867:Water
D009566:Nitrates; D014867:Water; D012965:Sodium Chloride; D002403:Cathepsins; D010450:Endopeptidases; D000626:Aminopeptidases; D005947:Glucose; D001205:Ascorbic Acid