title: The C terminus of the NF-kappa B p50 precursor and an I kappa B isoform contain transcription activation domains.
abstract: The p50 subunit of the NF-kappa B transcription complex is derived from the N-terminal half of a larger precursor protein, p105. Although a fair amount is known about functions located within the p50 sequences, less is known about the C-terminal half of p105. In this report, we have identified a potent transcription activation domain located in the C terminus of mouse p105. In addition, the I kappa B beta proteins chicken p40 and human MAD-3, proteins that are related to the p105 C terminus, strongly activated transcription in chicken cells and yeast when fused to GAL4 DNA-binding sequences. Furthermore, chicken p40 is primarily located in the nucleus of chicken cells when overexpressed from a retroviral vector. Our results suggest novel models for the function and regulation of NF-kappa B transcription complexes.
authors.affiliation: Department of Biology, Boston University, MA 02215.
authors.forename: P J
authors.initials: PJ
authors.lastname: Morin
authors.forename: T D
authors.initials: TD
authors.lastname: Gilmore
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D020672:I-kappa B Proteins; D051379:Mice; D008969:Molecular Sequence Data; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics*; D052002:NF-kappa B p50 Subunit; D010957:Plasmids / Q000235:genetics; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; C083487:I kappa B beta protein; D020672:I-kappa B Proteins; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; C000606204:NFKBIA protein, human; C000606205:Nfkbia protein, mouse; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000072000:NF-KappaB Inhibitor alpha