Evaluation of murine lymphocyte membrane potential, intracellular free calcium, and interleukin-2 receptor expression upon exposure to 1,1-dimethylhydrazine.
The effects of 1,1-dimethylhydrazine (UDMH) on several early events associated with lymphocyte activation were examined. The concentration of intracellular calcium ([Ca2+]i) and membrane potential of murine lymphocytes were found to be altered upon exposure to UDMH; [Ca2+]i was increased in murine thymocytes, while splenocytes exhibited membrane hyperpolarization. In addition, interleukin-2 receptor expression induced by in-vitro concanavalin A stimulation of murine splenocytes at 24 and 48 h in the presence of UDMH was not affected. UDMH may interfere with the ability of these two distinct lymphocyte populations to regulate normal ionic fluctuations, thus contributing to altered immune responsiveness.
Frazier
D E
DE
Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.
Tarr
M J
MJ
Olsen
R G
RG
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D004127:Dimethylhydrazines / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008564:Membrane Potentials; D051379:Mice; D008807:Mice, Inbred BALB C; D015375:Receptors, Interleukin-2 / Q000187:drug effects / Q000378:metabolism*; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology; D013997:Time Factors
D004127:Dimethylhydrazines; D015375:Receptors, Interleukin-2; D003208:Concanavalin A; C013958:dimazine; D002118:Calcium