[Beneficial effect of oxygen-free radical blockers in experimental kidney transplantation in rats].
The clinical potential of free radical (OFR) ablative therapy is dependent upon the proportion of the total injury caused by the reperfusion mechanism compared with the proportion resulting from ischemic injury itself. Prostaglandin cascade can both activate and be activated by OFR.


OBJECTIVE
to investigate the influence of different periods of cold ischemis in renal tissue (cortex and medulla) -regarding superoxide dismutase (S.O.D.) activity, the amount of erythrocyte trapping and prostaglandin synthesis. Also, to evaluate the effect of exogenous S.O.D. in the prevention of reperfusion injury.


METHODS
48 Lewis male rats (200-250 g) received renal isografts (RTx) preserved in Collins solution at 4 C for different periods: control group (8) non operated; group I (8) immediate RTx; group II (8) 12 hrs; group III (8) 18 hrs; group IV (8) 12 hrs+S.O.D. (13 mg/kg e.v.); group V (8) 18 hrs+S.O.D. (13 mg/kg e.v.). Before reperfusion all recipients received 1 ml of 51-Cr labelled erythrocytes. After 15 min. reperfusion grafts were removed and samples (cortex and medulla) obtained for measuring trapping of erythrocytes. S.O.D. activity and prostaglandins (PGe2, TxB2, 6-Keto-PGF1).


RESULTS
A strong correlation was found between the duration of cold ischemia and the amount of trapping both in cortex and in medulla. S.O.D. administration induced a significant drop of trapping. In non-operated rats S.O.D. activity in cortex was two fold medulla content. However, after reperfusion, a significant decrease in cortex was found in all groups. S.O.D. administration raised S.O.D. activity in cortex similar to control values.(ABSTRACT TRUNCATED AT 250 WORDS)
Alcaraz
A
A
Departamento de Cirugía, Hospital Clínico, Universidad de Barcelona.
López-Boado
M A
MA
Vicens
A
A
Talbot-Wright
R
R
Saenz
A
A
Carretero
P
P
Fernández-Cruz
L
L
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis; D000818:Animals; D016166:Free Radical Scavengers; D007668:Kidney / Q000737:chemistry; D016030:Kidney Transplantation; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D013482:Superoxide Dismutase / Q000032:analysis; D013929:Thromboxane B2 / Q000032:analysis
D016166:Free Radical Scavengers; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D013482:Superoxide Dismutase; D010100:Oxygen
Efecto beneficioso de los bloqueadores de radicales libres de oxígeno en el trasplante experimental de riñón en rata.