title: DNA hydrolyzing autoantibodies.
abstract: A DNA-nicking activity was detected in the sera of patients with various autoimmune pathologies and was shown to be a property of autoantibodies. The DNA hydrolyzing activity, which was purified by affinity and high-performance liquid chromatography, corresponded in size to immunoglobulin M (IgM) and IgG and had a positive response to antibodies to human IgG. The DNA hydrolyzing autoantibodies were stable to acid shock and yielded a DNA degradation pattern that was different from that of deoxyribonuclease (DNase) I and blood DNase.
authors.affiliation: V.A. Engelhardt Institute of Molecular Biology, Academy of Sciences of Russia, Moscow.
authors.forename: A M
authors.initials: AM
authors.lastname: Shuster
authors.forename: G V
authors.initials: GV
authors.lastname: Gololobov
authors.forename: O A
authors.initials: OA
authors.lastname: Kvashuk
authors.forename: A E
authors.initials: AE
authors.lastname: Bogomolova
authors.forename: I V
authors.initials: IV
authors.lastname: Smirnov
authors.forename: A G
authors.initials: AG
authors.lastname: Gabibov
mesh_terms: D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D001323:Autoantibodies / Q000302:isolation & purification / Q000378:metabolism*; D001327:Autoimmune Diseases / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism*; D004256:DNA Polymerase I / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000378:metabolism; D007075:Immunoglobulin M / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D010957:Plasmids
chemical_list: D000085:Acetates; D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D004247:DNA; D004256:DNA Polymerase I; D003850:Deoxyribonuclease I; D019342:Acetic Acid