A kinetic study of factor I-mediated modification and release of RBC-bound DNA-anti-DNA immune complexes.
A tritiated DNA-anti-DNA complement--containing immune complex probe was used to define the mechanism of factor I-mediated modification and release of erythrocyte CR1-bound immune complex in 10 normal control subjects. Experiments were performed with preformed immune complex, autologous erythrocytes, and autologous serum. Control experiments were performed by using a single donor source of erythrocytes and commercial factor I. The data demonstrate that the modification-release rate was first order with respect to factor I concentration, the mean half-life of red blood cell-bound immune complex was 1.7 +/- 0.7 minutes, and the mean percent of total immune complex present as unbound complexes was 19 +/- 11. These data are consistent with a clearance model in which factor I-mediated modification and release of red blood cell-bound immune complex plays a significant role in immune complex handling.
Meryhew
N L
NL
Department of Medicine, University of Minnesota, Minneapolis 55455.
Mukherjee
J
J
Creager
M S
MS
Runquist
O A
OA
D000974:Antibodies, Antinuclear / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D001692:Biological Transport; D004247:DNA / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D005340:Fibrinogen / Q000032:analysis / Q000494:pharmacology*; D006207:Half-Life; D006801:Humans; D008433:Mathematics
D000974:Antibodies, Antinuclear; D000936:Antigen-Antibody Complex; D005340:Fibrinogen; D004247:DNA