Monitoring the surface tension of lipid membranes by a bubble method.
A new method for the determination of lipid membrane surface tension was developed. Advantages of this method are that it allows for multiple measurements on a single membrane, is fast and direct not requiring empirical corrections, may be applied for dynamical surface-tension measurements and may be used with thin films and asymmetrical electrolytes. The pressure and radius of a bubble are measured. A piezoresistive sensor is used to minimize the transducer compliance. By moulding the sensor to a brass plate a resolution of 0.025 mm H2O (0.25 Pa) is obtained. The bubble is filmed using a videocamera and the radius of the bubble determined with the aid of a microcomputer. Data for monoolein/hexadecane in potassium chloride solutions and a cooling curve are presented and compared with previous results.
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Department of Physiology and Medical Biophysics, Biomedical Centre, Uppsala, Sweden.
D008051:Lipid Bilayers / Q000737:chemistry*; D008722:Methods; D013500:Surface Tension
D008051:Lipid Bilayers