title: Effects of L-histidine on hydrogen peroxide-induced DNA damage and cytotoxicity in cultured mammalian cells.
abstract: L-Histidine markedly increased the growth- and DNA synthesis-inhibitory effects elicited by hydrogen peroxide in cultured Chinese hamster ovary cells. DNA single-strand breakage was also higher in the presence of the amino acid and, in addition, these breaks were characterized by a slower rate of repair, compared with that of the breaks generated by the oxidant alone. In the presence of L-histidine, hydrogen peroxide also produced DNA double-strand breakage, a lesion that cannot be detected in cells treated with even exceedingly high concentrations of the oxidant alone. Data reported herein suggest that the L-histidine-mediated increase of the cytotoxic response of cultured Chinese hamster ovary cells to hydrogen peroxide may be at least partially dependent on the formation of DNA double-strand breaks.
authors.affiliation: Istituto di Farmacologia e Farmacognosia, Università degli Studi di Urbino, Italy.
authors.forename: O
authors.initials: O
authors.lastname: Cantoni
authors.forename: P
authors.initials: P
authors.lastname: Sestili
authors.forename: A
authors.initials: A
authors.lastname: Guidarelli
authors.forename: P U
authors.initials: PU
authors.lastname: Giacomoni
authors.forename: F
authors.initials: F
authors.lastname: Cattabeni
mesh_terms: D000818:Animals; D016466:CHO Cells; D002250:Carbon Radioisotopes; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006639:Histidine / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics
chemical_list: D002250:Carbon Radioisotopes; D006639:Histidine; D004247:DNA; D006861:Hydrogen Peroxide