title: Gap junctional conductance in ventricular myocyte pairs isolated from postischemic rabbit myocardium.
abstract: Abnormalities of myocardial gap junction-mediated cell coupling have been implicated in cardiac arrhythmogenesis. The potential role of gap junctional dysfunction in the generation of reperfusion-induced arrhythmias is uncertain. The purpose of this study was to measure the effects of myocardial ischemia and reperfusion on gap junctional conductance (gj) between isolated ventricular myocytes. By using a new experimental model, myocyte pairs were isolated from Langendorff-perfused rabbit hearts 1) after 30 minutes of global normothermic ischemia followed by 30 minutes of reperfusion, 2) after 75 minutes of control perfusion, or 3) immediately after removal of the heart. Myocytes and myocyte pairs were studied using whole-cell recording techniques. Action potential characteristics of cells in all three groups were normal. Despite similar mean gj in all three groups (0.88 +/- 0.27, 1.15 +/- 0.18, and 1.24 +/- 0.25 microS, respectively; p greater than 0.05), the postischemic group was more widely distributed and had a significantly greater proportion of poorly communicating cell pairs than either control group (gj less than 25% of mean in eight of 15 myocyte pairs versus zero of 15 and one of 13, respectively; p less than 0.02). Thus, postischemic myocyte pairs represent a heterogeneous population of electrically coupled cells in which individual deficits in coupling are masked by a normal mean value. In the reperfused intact heart, local disturbances of cell coupling, similarly undetected by gross measures of conduction, could disrupt myocardial conduction and activation on a microscopic scale and thus enhance arrhythmogenicity.
authors.affiliation: Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia.
authors.forename: R S
authors.initials: RS
authors.lastname: Kieval
authors.forename: J F
authors.initials: JF
authors.lastname: Spear
authors.forename: E N
authors.initials: EN
authors.lastname: Moore
mesh_terms: D000200:Action Potentials; D000818:Animals; D003327:Coronary Disease / Q000473:pathology / Q000503:physiopathology*; D004553:Electric Conductivity; D005260:Female; D006321:Heart / Q000503:physiopathology*; D007365:Intercellular Junctions / Q000502:physiology*; D008297:Male; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000473:pathology*; D011817:Rabbits; D012016:Reference Values