title: Expression of alpha-cardiac myosin heavy chain in mammalian skeletal muscle.
abstract: We have investigated the reactivity of different human, rat and cat muscles to a monoclonal antibody directed against human alpha-cardiac myosin heavy chain. We have found that special fiber subpopulations of human masseter and extraocular muscles, as well as the bag fibers of human, rat and cat muscle spindles, were reactive to this antibody, indicating that these fibers expressed alpha-cardiac myosin heavy chain or a closely related isoform. This isomyosin was present in the spindle bag fibers at early fetal stages, whereas its expression in masseter and extraocular muscle fibers was not detected during the first 22 weeks of gestation. Our results add to the list of muscle proteins which are expressed in locations or at developmental stages other than those initially described, suggesting that a revision of the present nomenclature of the subgroups of myosin heavy chains might be considered in the future.
authors.affiliation: Department of Anatomy, University of Umeå, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Pedrosa-Domellöf
authors.forename: P O
authors.initials: PO
authors.lastname: Eriksson
authors.forename: G S
authors.initials: GS
authors.lastname: Butler-Browne
authors.forename: L E
authors.initials: LE
authors.lastname: Thornell
mesh_terms: D000328:Adult; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D002415:Cats; D002648:Child; D002675:Child, Preschool; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D024510:Muscle Development; D009132:Muscles / Q000196:embryology / Q000378:metabolism; D009218:Myosins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000911:Antibodies, Monoclonal; D009218:Myosins