Phosphate starvation enhances expression of the immunodominant 38-kilodalton protein antigen of Mycobacterium tuberculosis: demonstration by immunogold electron microscopy.
In this work, we grew Mycobacterium tuberculosis in an enriched Proskauer-Beck-Youmans culture medium in the presence and in the absence of phosphate salts. Immunoblot analysis of sonic extracts showed overexpression of the 38-kDa protein antigen by bacilli grown in the medium without phosphate. These observations were confirmed by immunogold electron microscopy, which showed that the number of gold particles was significantly higher in bacilli grown in medium without phosphate than in bacilli grown in medium with phosphate. The 38-kDa protein was located mainly in the wall and on the cell surface.
Espitia
C
C
Departamento de Inmunología, Universidad Nacional Autónoma de México, Mexico City.
Elinos
M
M
Hernández-Pando
R
R
Mancilla
R
R
D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000302:isolation & purification; D002462:Cell Membrane / Q000276:immunology; D015870:Gene Expression / Q000187:drug effects*; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010710:Phosphates / Q000494:pharmacology*
D000942:Antigens, Bacterial; D010710:Phosphates