Ranking toxicity of industrial dusts by bronchoalveolar lavage fluid analysis.
Female wistar rats were inoculated intratracheally with 10 mg/ml suspensions of various dusts, viz: quartz, fly ash, mica and corundum in physiological saline. Biochemical markers of bronchoalveolar lavage fluid (BALF) were analysed 8 days after the instillation of the dusts. Elevated levels of proteins, sialic acid and phospholipid contents and the activity of lactate dehydrogenase correlated well with the degree of the known fibrogenic potential of different dusts in the lungs in the following order, quartz greater than fly ash greater than mica greater than corundum. beta-glucuronidase activity, was however, only elevated in the quartz inoculated group of rats. It is suggested that biochemical constituents of BALF analysed shortly after the exposure to different dusts can be useful to mirror alterations in the tissue response to mineral dusts.
Bajpai
R
R
Industrial Toxicology Research Centre, Lucknow, India.
Waseem
M
M
Gupta
G S
GS
Kaw
J L
JL
D000537:Aluminum Oxide / Q000633:toxicity; D000538:Aluminum Silicates / Q000633:toxicity; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000201:enzymology; D003031:Coal / Q000633:toxicity; D004391:Dust / Q000009:adverse effects*; D005260:Female; D005966:Glucuronidase / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008168:Lung / Q000187:drug effects*; D019158:N-Acetylneuraminic Acid; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011791:Quartz / Q000633:toxicity; D051381:Rats; D012794:Sialic Acids / Q000032:analysis
D000538:Aluminum Silicates; D003031:Coal; D004391:Dust; D010743:Phospholipids; D011506:Proteins; D012794:Sialic Acids; D011791:Quartz; D007770:L-Lactate Dehydrogenase; D005966:Glucuronidase; D019158:N-Acetylneuraminic Acid; D000537:Aluminum Oxide; C011934:mica