Depressed adjuvant arthritis in chronically Trypanosoma cruzi infected rats: reversal by cyclophosphamide.
Chronically Trypanosoma cruzi infected "I" rats and syngeneic naive recipients, transferred with a T cell enriched spleen cell population from infected donors, develop an attenuated arthritis when challenged with complete Freund's adjuvant. We report that cyclophosphamide, 40 mg/kg body weight, given 48 h before induction, was able to reestablish or exacerbate adjuvant arthritis in infected and control rats, respectively. Although the T cell enriched spleen cells from infected donors continued to down regulate adjuvant arthritis in syngeneic recipients given cyclophosphamide 48 h before cell transfer, treatment of infected donors with cyclophosphamide, 48 h before cell collection, prevented these cells from exerting such effect when transferred to healthy recipients receiving no cyclophosphamide. It is suggested that cyclophosphamide may primarily affect a suppressor cell population, present in the infected host, with regulatory activity on adjuvant arthritis.
Revelli
S
S
Immunology Division, Faculty of Medical Sciences, Universidad Nacional de Rosario, Argentina.
Dávila
H
H
Moreno
H
H
Bottasso
O
O
D000818:Animals; D001169:Arthritis, Experimental / Q000382:microbiology*; D014355:Chagas Disease; D002908:Chronic Disease; D003520:Cyclophosphamide / Q000494:pharmacology*; D016219:Immunotherapy, Adoptive; D008297:Male; D051381:Rats; D013154:Spleen / Q000166:cytology / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology; D014349:Trypanosoma cruzi
D003520:Cyclophosphamide