Cloning and expression of Staphylococcus epidermidis urease gene sequences in Staphylococcus carnosus.
The urease gene sequences of Staphylococcus epidermidis CNS23 were cloned and expressed in Staphylococcus carnosus TM300. In vitro translation of the cloned sequences revealed four polypeptides (60, 17, 11 and 7.5 kDa) that were associated with enzyme activity. Southern hybridisation experiments showed high homologies with the urease genes of Staphylococcus saprophyticus.
Grüter
L
L
Institut für Medizinische Mikrobiologie und Immunologie, Universität Hamburg, FRG.
Endres
M
M
Gatermann
S
S
D015139:Blotting, Southern; D003001:Cloning, Molecular; D015870:Gene Expression; D005798:Genes, Bacterial; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013210:Staphylococcus / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000201:enzymology / Q000235:genetics*; D014510:Urease / Q000235:genetics* / Q000378:metabolism
D014510:Urease