Preparation of B lymphocyte-specific alloantisera by skin implant immunization of cattle.
B lymphocyte-specific antisera were prepared by immunizing cattle on either one or two occasions with a subcutaneous implant of allogeneic skin and subsequently absorbing the antisera with platelets. After absorption 15/26 antisera displayed B lymphocyte-specific activity. Titres against B-enriched cells were 8-64 while residual titres against B-depleted cells were 1-8. In comparison, 3-6 immunizations with allogeneic leucocytes produced antisera of similar peak cytotoxic titres against donor PBL, and after platelet absorption 8/15 antisera displayed B lymphocyte-specific activity. Titres against B-enriched cells were 8-64 while residual titres against B-depleted cells were 2-8. The skin implant method was less time-consuming than the leucocyte immunization method.
Mackie
J T
JT
Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra, A.C.T.
Stear
M J
MJ
D000818:Animals; D000918:Antibody Specificity / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology; D002417:Cattle; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D007114:Immunization; D007518:Isoantibodies / Q000096:biosynthesis*; D007962:Leukocytes / Q000276:immunology; D016038:Skin Transplantation / Q000276:immunology*
D007518:Isoantibodies