title: Evaluation of a monoclonal antibody, BC-1, which identifies an antigen expressed on the surface membrane of human extravillous trophoblast.
abstract: This paper describes a new Mab BC-1 which is directed at a cell surface antigen expressed by extravillous cytotrophoblast and not by villous cytotrophoblast, so it is useful for distinguishing between the two populations. The antigen recognised by BC-1 is trypsin-resistant, which allows it to be used for flow cytometric analysis of living trophoblast isolated by enzymic disaggregation. However, BC-1 is not trophoblast-specific but cross-reacts with some other tissues, in particular endothelial cells and peripheral blood monocytes. The nature of this antigen has not been established.
authors.affiliation: Department of Pathology, University of Cambridge, United Kingdom.
authors.forename: Y W
authors.initials: YW
authors.lastname: Loke
authors.forename: B L
authors.initials: BL
authors.lastname: Hsi
authors.forename: J N
authors.initials: JN
authors.lastname: Bulmer
authors.forename: C
authors.initials: C
authors.lastname: Grivaux
authors.forename: S
authors.initials: S
authors.lastname: Hawley
authors.forename: L
authors.initials: L
authors.lastname: Gardner
authors.forename: A
authors.initials: A
authors.lastname: King
authors.forename: N P
authors.initials: NP
authors.lastname: Carter
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015415:Biomarkers; D002478:Cells, Cultured; D003429:Cross Reactions; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D009928:Organ Specificity; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014327:Trophoblasts / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers