Nucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline protease of Aspergillus fumigatus.
An Aspergillus fumigatus extracellular alkaline protease (ALP) which is an enzyme of the subtilisin family is a potential virulent factor of the fungus. The gene encoding ALP was isolated from a genomic library made from DNA of an A. fumigatus isolate. The nucleotide sequence of this gene was compared to that of a cDNA encoding A. oryzae ALP and to that of a cDNA from A. fumigatus encoding the mature ALP protein. Mature A. fumigatus ALP contains 282 amino acids and is encoded by three exons. The pre-proenzyme has a leader sequence of 121 amino acids.
Jaton-Ogay
K
K
Laboratoire de mycologie, Centre hospitalier universitaire vaudois, Lausanne, Switzerland.
Suter
M
M
Crameri
R
R
Falchetto
R
R
Fatih
A
A
Monod
M
M
D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D005091:Exons / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism
D015342:DNA Probes; D012697:Serine Endopeptidases; C032923:microbial serine proteinases