[Amounts of urinary metabolites of p-chloroaniline and their half lives in a patient with acute poisoning].
The aim of this study was to elucidate the time courses of amounts of urinary p-chloroaniline (p-CA) metabolites during acute poisoning and to establish a suitable method for biological monitoring of exposure to p-CA. Urinary p-CA metabolites which were previously identified in a patient with acute p-CA poisoning were determined quantitatively over time during admission of a patient by high-performance liquid chromatography (HPLC) and by the colorimetric method of diazo-positive metabolites (DPM). The data of urinary excretion of each p-CA metabolite were fitted to two-compartment model of pharmacokinetics. Major urinary metabolites of p-CA were conjugated p-CA and conjugated 2-amino-5-chlorophenol (2-A-5-CP), and the detected amounts of 2,4-dichloroaniline (2,4-DCA) and free p-CA were small. During the rapid phase for the disappearance of each metabolite, half lives of p-CA, 2-A-5-CP and 2,4-DCA were 2.4, 1.7 and 1.7 h, respectively, and during the slow phase, they were 4.5, 3.3 and 3.8 h, respectively. Urinary p-CA occupied about 62% of the total metabolites detected in the urine, 2-A-5-CP about 36%, and 2,4-DCA about 1%. 2-A-5-CP and p-CA were detected in the urine of the patient obtained on days 3 and 4, respectively, after the poisoning, but the amount of DPM after the second day of the poisoning was in the range of that of non-exposed persons.(ABSTRACT TRUNCATED AT 250 WORDS)
Yoshida
T
T
Department of Occupational Health, Osaka Prefectural Institute of Public Health.
Hirata
M
M
Tabuchi
T
T
Miyajima
K
K
Andoh
K
K
D000208:Acute Disease; D000814:Aniline Compounds / Q000493:pharmacokinetics / Q000506:poisoning* / Q000652:urine; D002733:Chlorophenols / Q000493:pharmacokinetics / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D004784:Environmental Monitoring; D006207:Half-Life; D006801:Humans
D000814:Aniline Compounds; D002733:Chlorophenols; C054619:2,4-dichloroaniline; C004658:4-chloroaniline