A modified alkaline phosphatase enzyme amplification system and its application in an HIV antigen ELISA.
Alkaline phosphatase amplification systems increase the sensitivity of enzyme-linked immunoassays (ELISAs). Here we describe a modified method by which levamisole is a component of the substrate buffer. This increases sensitivity by reducing the signal from non-specific alkaline phosphatases. The modified procedure was applied to an 'in house' HIV-1 p24 antigen capture assay and was shown to increase the sensitivity 4-fold as compared to the unmodified system. The performance of the modified amplification system is comparable to that of commercially available systems.
Ball
J K
JK
Regional Virus Laboratory, East Birmingham Hospital, U.K.
Beards
G M
GM
Desselberger
U
U
D000469:Alkaline Phosphatase / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D016655:HIV Core Protein p24 / Q000032:analysis*; D007978:Levamisole; D013379:Substrate Specificity
D016655:HIV Core Protein p24; D007978:Levamisole; D000469:Alkaline Phosphatase