Cyclosporin-A increases type I procollagen production and mRNA level in human gingival fibroblasts in vitro.
In order to study the pathogenesis of gingival overgrowth induced by the immunosuppressive drug cyclosporine-A (CyA), we investigated its effect on 3H thymidine incorporation and on collagen production and mRNA levels in fibroblast cultures obtained from normal human gingiva. At concentrations of 100, 500 and 1000 ng/ml, CyA did not modify thymidine incorporation after 24 and 72 h of incubation. However, after 24 h it significantly increased the level of 3H proline-containing proteins in the medium. In addition, CyA increased alpha-procollagen chains by up to three times. This CyA-induced change was related to a rise in the level of type I procollagen. The CyA effect on fibroblasts was markedly reduced by cycloheximide, an inhibitor of protein synthesis, and it correlated well with an increase of type I procollagen mRNA. Overall, our data indicate a direct stimulatory action of CyA on collagen synthesis, but not on DNA synthesis, in human gingival fibroblasts.
Schincaglia
G P
GP
Istituto di Chimica Biologica, Ferrara, Italy.
Forniti
F
F
Cavallini
R
R
Piva
R
R
Calura
G
G
del Senno
L
L
D003094:Collagen / Q000096:biosynthesis; D016572:Cyclosporine / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005885:Gingival Hyperplasia / Q000139:chemically induced*; D006801:Humans; D066298:In Vitro Techniques; D011347:Procollagen / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
D011347:Procollagen; D012333:RNA, Messenger; D016572:Cyclosporine; D003094:Collagen