title: Sex determination of forensic samples by dual PCR amplification of an X-Y homologous gene.
abstract: Sex determination by polymerase chain reaction (PCR) analysis of the X-Y homologous amelogenin gene is highly reliable since the detection of an X-specific amplified fragment validates the procedure. Previously, we reported that 250 ng of template DNA are required for sex determination by this method. We report here a refinement of the technique to include dual PCR. Dual PCR using two sets of primers results in the detection of X- and Y-specific amplified fragments from as little as 0.005 ng of template DNA. This is a powerful technique for the analysis of trace forensic samples and its application is discussed.
authors.affiliation: Department of Legal Medicine, Shimane Medical University, Izumo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Akane
authors.forename: S
authors.initials: S
authors.lastname: Seki
authors.forename: H
authors.initials: H
authors.lastname: Shiono
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
authors.forename: M
authors.initials: M
authors.lastname: Kagawa
authors.forename: K
authors.initials: K
authors.lastname: Matsubara
authors.forename: Y
authors.initials: Y
authors.lastname: Nakahori
authors.forename: S
authors.initials: S
authors.lastname: Nagafuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakagome
mesh_terms: D053523:Amelogenin; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D003746:Dental Enamel Proteins / Q000235:genetics*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D005784:Gene Amplification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012730:Sex Chromosomes / Q000737:chemistry*; D012732:Sex Determination Analysis; D013698:Templates, Genetic; D014960:X Chromosome / Q000737:chemistry; D014998:Y Chromosome / Q000737:chemistry
chemical_list: D053523:Amelogenin; D003746:Dental Enamel Proteins; D004247:DNA