Analysis of drugs by polarography, XXXV: The polarographic behaviour of cinolazepam[1-(2-cyanoethyl)-7-chloro-3-hydroxy-5-(2-fluorophe n yl)-1,3-dihydro-2H-1,4-benzodiazepin-2-one] and assay of its tablets.
Cinolazepam is reduced at the Dropping Mercury Electrode (DME) over the entire pH range in Britton Robinson buffers (BRB) containing 20% DMF as a solubilizer. A well-defined cathodic wave is obtained over the pH range 3-5, which is very suitable for analytical purposes. The wave was characterized as being irreversible, diffusion-controlled and free from adsorption phenomena. In acid buffers, 4 electrons are consumed in the reduction process, while in alkaline buffers the electrode reaction is somewhat complicated. Tablets containing 40 mg cinolazepam were analysed using the polarographic and a developed HPLC method. The results obtained with both methods are in good accordance as revealed by statistical analysis. The details of the complicated mechanism of the reduction process at the DME are still under investigation but elucidated in principle.
Oelschläger
H
H
Institut für Pharmazeutische Chemie, Johann Wolfgang Goeth-Universität Frankurt am Main, Germany.
Volke
J
J
Belal
F
F
D014151:Anti-Anxiety Agents; D001569:Benzodiazepines / Q000032:analysis*; D004563:Electrochemistry; D011048:Polarography; D013607:Tablets
D014151:Anti-Anxiety Agents; D013607:Tablets; D001569:Benzodiazepines; C053781:cinolazepam