Isolation and characterization of a Vicia graminea and Vicia unijuga lectins-binding (Vgu) glycoprotein with Thomsen-Friedenreich (T) activity from human liver metastases of pancreas carcinoma.
1. Perchloric acid-soluble fraction from liver metastases of pancreas carcinoma of a patient with blood group B, was subjected to a systematic affinity chromatography using Vicia unijuga lectin (VUA) and Arachis hypogaea anti-T lectin (PNA) as immobilized ligands and separated into three fractions, B-active glycoprotein fraction, serologically inactive glycoprotein fraction and glycoprotein (VP) fraction exhibiting reactivities for Vicia graminea lectin (VGA), VUA and PNA. 2. VGA- and VUA-binding (Vgu) glycoprotein with Thomsen-Friedenreich (T) activity which was isolated, in a high state of purity, from VP fraction by HPLC using Asahipak GS-710 column, was demonstrated to be a mannose-rich glycoprotein with mol. wt of 1492 kDa and contained 40.40% carbohydrate.
Ueno
E
E
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Otsuka
K
K
Kudo
T
T
Ohkuma
S
S
D015295:Antigens, Tumor-Associated, Carbohydrate; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000302:isolation & purification*; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism* / Q000556:secondary; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000473:pathology*; D037121:Plant Lectins
D015295:Antigens, Tumor-Associated, Carbohydrate; D004187:Disaccharides; D006023:Glycoproteins; D037102:Lectins; D009363:Neoplasm Proteins; D037121:Plant Lectins; C042242:Vicia lectins; C016467:Thomsen-Friedenreich antigen