title: Ultraviolet radiation (UVR) induces cell-surface Ro/SSA antigen expression by human keratinocytes in vitro: a possible mechanism for the UVR induction of cutaneous lupus lesions.
abstract: Antinuclear antibodies are useful markers of connective tissue disease. In this study, UVB but not UVA induced the expression of Ro/SSA antigen on keratinocyte surfaces in vitro. This expression was also found with the extractable nuclear antigens RnP and Sm, but not with single or double-stranded DNA. The expression was prevented by blocking protein synthesis, suggesting that it was an active process. The results suggest that UVB exposure may result in the expression of Ro/SSA antigen on the surfaces of basal keratinocytes in vivo. This antigen could then bind circulating antibody leading to the cutaneous lesions in neonatal and subacute cutaneous lupus erythematosus.
authors.affiliation: Department of Dermatology, Bristol Royal Infirmary, U.K.
authors.forename: S K
authors.initials: SK
authors.lastname: Jones
mesh_terms: D000954:Antigens, Surface / Q000528:radiation effects; D001324:Autoantigens / Q000528:radiation effects*; D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000276:immunology / Q000528:radiation effects*; D008180:Lupus Erythematosus, Systemic / Q000209:etiology* / Q000276:immunology; D008856:Microscopy, Fluorescence; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D000099207:SS-A Antigen
chemical_list: D000954:Antigens, Surface; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human