title: X-ray analyses of peptide-inhibitor complexes define the structural basis of specificity for human and mouse renins.
abstract: X-ray analyses have defined the three-dimensional structures of crystals of mouse and human renins complexed with peptide inhibitors at resolutions of 1.9 and 2.8 A, respectively. The exquisite specificity of renin arises partly from ordered loop regions at the periphery of the binding cleft. Although the pattern of main-chain hydrogen bonding in other aspartic proteinase inhibitor complexes is conserved in renins, differences in the positions of secondary structure elements (particularly helices) also lead to improved specificity in renins for angiotensinogen substrates.
authors.affiliation: Department of Crystallography, Birkbeck College, London, UK.
authors.forename: V
authors.initials: V
authors.lastname: Dhanaraj
authors.forename: C G
authors.initials: CG
authors.lastname: Dealwis
authors.forename: C
authors.initials: C
authors.lastname: Frazao
authors.forename: M
authors.initials: M
authors.lastname: Badasso
authors.forename: B L
authors.initials: BL
authors.lastname: Sibanda
authors.forename: I J
authors.initials: IJ
authors.lastname: Tickle
authors.forename: J B
authors.initials: JB
authors.lastname: Cooper
authors.forename: H P
authors.initials: HP
authors.lastname: Driessen
authors.forename: M
authors.initials: M
authors.lastname: Newman
authors.forename: C
authors.initials: C
authors.lastname: Aguilar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003460:Crystallization; D015195:Drug Design; D006801:Humans; D006860:Hydrogen Bonding; D051379:Mice; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D011480:Protease Inhibitors / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D012083:Renin / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity; D014961:X-Ray Diffraction; D000092502:Renin Inhibitors
chemical_list: D009842:Oligopeptides; D011480:Protease Inhibitors; D012083:Renin; D000092502:Renin Inhibitors; C075110:CP 85339; C071116:CH 66