[Isolation of mycobacteria from sputum. Comparison of the MB Check with the radiometric and conventional method].
1179 sputum samples were investigated by the MB-Check, the conventional method and Bactec 460 to compare the recovery rate of Mycobacterium tuberculosis, the contamination rate, and time requirement for the isolation. Of the 293 positive sputa, the MB-Check detected 89,1%, Löwenstein Jensen medium 90,1%, the Bactec-system 90,4%, the combination MB-Check/Löwenstein Jensen 95,9%; and the combination Bactec/Löwenstein Jensen 96,6%. The average detection times for 130 Mycobacterium tuberculosis-strains with the MB-Check slide, with the liquid medium of the MB-Check, with Löwenstein Jensen and with Bactec were 26.0, 20.4, 25.3, and 12.0 days, respectively.
Rüsch-Gerdes
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Forschungsinstitut Borstel.
Jährling
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A
D001431:Bacteriological Techniques / Q000295:instrumentation*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D009170:Nontuberculous Mycobacteria / Q000302:isolation & purification*; D011874:Radiometry; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
Isolierung von Mykobakterien aus Sputum. Vergleich des MB-Checks mit der radiometrischen und der konventionellen Methode.