The effect of K+/H+ antiporter nigericin on gap junction permeability.
The K+/H+ antiporter nigericin inhibits the intercellular exchange of the fluorescent dye Lucifer Yellow between DM15-transformed fibroblasts derived from the Djungarian hamster. The efficacy of nigericin action was related to its concentration and time of incubation. The nigericin-induced uncoupling effect on gap junctions was reversible and was shown to be based on its ability to cause cystolic acidification. The effect of nigericin on dye-coupling in intact and 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-pretreated cells did not differ, indicating that the uncoupling effect of H+ on gap junctions in DM15 cells was not mediated by the TPA-dependent isoform of protein kinase C.
Budunova
I V
IV
Laboratory of Carcinogen Screening Methods, All-Union Cancer Research Center of the AMS USSR Moscow.
Mittelman
L A
LA
D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology*; D002461:Cell Line, Transformed; D006224:Cricetinae; D005347:Fibroblasts / Q000187:drug effects; D005456:Fluorescent Dyes; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D007365:Intercellular Junctions / Q000187:drug effects*; D007546:Isoquinolines / Q000378:metabolism; D009550:Nigericin / Q000494:pharmacology*; D010539:Permeability / Q000187:drug effects; D011188:Potassium; D027961:Potassium-Hydrogen Antiporters; D011493:Protein Kinase C / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002352:Carrier Proteins; D005456:Fluorescent Dyes; D007546:Isoquinolines; D027961:Potassium-Hydrogen Antiporters; D006859:Hydrogen; C017475:lucifer yellow; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D009550:Nigericin; D011188:Potassium