[Expression of hemidesmosome-associated antigen and its biochemical properties].
Immunohistochemical study on the hemidesmosomes of the normal and malignant squamous epithelia of the human oral mucosa was performed using a monoclonal antibody (MoAb) 3A1 which recognized specifically the hemidesmosomes. Biochemical characterization of the antigen recognized by this antibody was also investigated. The results and conclusion of this study were as follows: 1) MoAb 3A1 reacted specifically with the hemidesmosomal plaques located on the basement membrane zone of the basal cells of the normal squamous epithelium. 2) In the cases of malignant epithelium, MoAb 3A1 reacted irregularly with the cell membrane at the border of the cell and stroma. The expression of the antigen in the carcinoma cells was found on the hemidesmosomes with abnormal structure. 3) The antigen recognized by MoAb 3A1 extracted from the cultured carcinoma cells was purified by protein A Sepharose affinity chromatography and DEAE ion-exchange HPLC. The purified antigen was a protein associated with hyaluronate having a molecular weight of more than 200 Kd. The molecular weight of the protein itself was found to be 180 Kd. Analysis of the amino acid composition showed this antigen was mainly composed of glycine, serine, alanine, glutamic acid and leucine.
Sashide
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First Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tokyo Medical and Dental University.
D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002448:Cell Adhesion; D003896:Desmosomes / Q000737:chemistry* / Q000276:immunology; D004586:Electrophoresis; D006801:Humans; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D009062:Mouth Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000941:Antigens