Inflammatory cells and eicosanoid mediators in subjects with late asthmatic responses and increases in airway responsiveness.
To determine the relationship of inflammatory cells and eicosanoid mediators to the pathogenesis of the late asthmatic response (LAR) and increases in nonspecific airway responsiveness, we studied bronchoalveolar lavage (BAL) cells and fluid in 27 subjects 12 hours after inhaled antigen challenge. Methacholine challenge was performed before antigen challenge and 24 hours later (12 hours after BAL). Eight subjects had no LAR (-LAR, less than or equal to 10% fall in FEV1), nine subjects had an equivocal LAR (+/- LAR, 11% 25% fall in FEV1), and 10 subjects had a definite LAR (+LAR, greater than 25% fall in FEV1). Subjects developing +LAR had increased airway responsiveness at baseline compared with that of subjects developing an +/- LAR, but not with subjects having -LAR. If airway responsiveness was markedly increased at baseline, further increases after antigen challenge were often not observed. We found that both percent neutrophils and eosinophils increased in BAL as the severity of the LAR increased, but significant differences between the groups with -LAR and +LAR were only observed when both cell types were considered together. In addition, there was a significant correlation between the combined cell percentages and the severity of the LAR as determined by fall in FEV1. Likewise, increases in airway responsiveness were associated with significant increases in both neutrophil and eosinophil numbers, but only neutrophils correlated with the change in airway responsiveness after antigen challenge. However, despite the significant physiologic and cellular differences that we found between our groups, no significant differences could be found in BAL eicosanoid-mediator concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)
Smith
H R
HR
Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Larsen
G L
GL
Cherniack
R M
RM
Wenzel
S E
SE
Voelkel
N F
NF
Westcott
J Y
JY
Bethel
R A
RA
D000403:Airway Resistance / Q000502:physiology*; D000704:Analysis of Variance; D001249:Asthma / Q000453:epidemiology / Q000503:physiopathology*; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D001999:Bronchoscopy; D002452:Cell Count; D015777:Eicosanoids / Q000032:analysis / Q000502:physiology*; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D007249:Inflammation / Q000453:epidemiology / Q000503:physiopathology; D008297:Male; D016210:Methacholine Chloride; D012882:Skin Tests
D015777:Eicosanoids; D016210:Methacholine Chloride