In vitro study of platelets and circulating mononuclear cells of subjects presenting an intolerance to aspirin.
Aspirine-sensitive asthma (ASA) is a disease defined only by clinical criteria. It is an intrinsic asthma related to a hypersensitivity to aspirin. The illness is linked to abnormalities in platelet and macrophage arachidonic acid metabolism. We assessed in vitro the platelet chemoluminescence (CL) and lymphocyte proliferative response of ASA patients. We observed that platelets from patients and control do not generate any CL in the presence of aspirin. Concerning the proliferative response of lymphocytes, the in vitro effect of aspirin depends upon the origin of the lymphocytes tested. Thus aspirin clearly enhances the proliferative response of lymphocytes from normal subjects but diminishes the thymidine uptake by lymphocytes from ASA patients. This discrepancy in the in vitro response of lymphocytes from normal subjects and patients might be useful for in vitro diagnosis of ASA.
Guez
S
S
Service de Médecine interne et Maladies allergiques, Hôpital Pellegrin, Bordeaux, France.
Gualde
N
N
Bezian
J H
JH
Cabanieu
G
G
D000368:Aged; D016718:Arachidonic Acid / Q000378:metabolism; D001241:Aspirin / Q000276:immunology*; D001249:Asthma / Q000139:chemically induced / Q000473:pathology; D001792:Blood Platelets / Q000187:drug effects*; D004342:Drug Hypersensitivity / Q000473:pathology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007660:Ketoprofen / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008163:Luminescent Measurements; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D012457:Salicylamides / Q000494:pharmacology
D012457:Salicylamides; D016718:Arachidonic Acid; D007660:Ketoprofen; C031060:salicylamide; D001241:Aspirin