A rapid polymerase chain reaction method for identifying fixed specimens.
DNA profiling analysis of preserved tissue samples was achieved by using an extraction method which incorporated the use of a chelating resin "Chelex" to chelate inhibitory polyvalent metal ions. An automated sequencer was used to estimate sizes of low molecular weight trimeric and tetrameric microsatellites labelled with fluorescent dyes. The entire analysis can be completed within 48h. Probabilities of chance association using two microsatellites were calculated as ranging between 10(-2) and 10(-4). The methods described have considerable potential for use in routine genetic analysis of preserved samples.
Gill
P
P
Central Research and Support Establishment, Aldermaston, Reading, Berkshire, England.
Kimpton
C P
CP
Sullivan
K
K
D000483:Alleles; D001483:Base Sequence; D002614:Chelating Agents; D004247:DNA / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D007475:Ion Exchange Resins; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011137:Polystyrenes; D011145:Polyvinyls; D011471:Prostatic Neoplasms / Q000235:genetics; D014021:Tissue Preservation
D002614:Chelating Agents; D004273:DNA, Neoplasm; D004276:DNA, Satellite; D007475:Ion Exchange Resins; D011137:Polystyrenes; D011145:Polyvinyls; C006960:chelex; D004247:DNA