title: Nucleotide sequence of the methoxyneurosporene dehydrogenase gene from Rhodobacter sphaeroides: comparison with other bacterial carotenoid dehydrogenases.
abstract: The nucleotide sequence of the 1794-bp fragment containing the crtD gene from Rhodobacter sphaeroides 2.4.1 encoding for methoxyneurosporene dehydrogenase has been determined. A 63% sequence identity was found when compared with the nucleotide sequence of the crtD gene from Rhodobacter capsulatus. A putative regulatory palindromic motif present in the crtD gene from R. capsulatus also exists in this gene from R. sphaeroides. The translated open reading frame of the crtD gene of R. sphaeroides has identified a polypeptide of 495 amino acids which shares a 56% sequence identity with the same CrtD protein of R. capsulatus. The N- and C-termini of these CrtD proteins present a high degree of similarity with the N- and C-termini of other carotenoid dehydrogenases including those encoded by crtI genes. This is in good agreement with the previously hypothesized homology between CrtI and CrtD proteins.
authors.affiliation: Department of Genetics and Microbiology, Faculty of Sciences, Autonomous University of Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Garí
authors.forename: J C
authors.initials: JC
authors.lastname: Toledo
authors.forename: I
authors.initials: I
authors.lastname: Gibert
authors.forename: J
authors.initials: J
authors.lastname: Barbé
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002338:Carotenoids / Q000096:biosynthesis; D003062:Codon; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D016354:Rhodobacter capsulatus / Q000201:enzymology / Q000235:genetics; D012242:Rhodobacter sphaeroides / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003062:Codon; D002338:Carotenoids; D010088:Oxidoreductases; C075277:methoxyneurosporene dehydrogenase