Effect of chloramphenicol on sister chromatid exchange in bovine fibroblasts.
The genotoxic potential of different chloramphenicol concentrations (5, 20, 40 and 60 micrograms ml-1) was investigated in bovine fibroblast primary lines by sister chromatid exchange assay. Chloramphenicol acted for long enough to ensure similar effects to persistent storage in the kidney. In this experiment 10 micrograms ml-1 of 5-bromodeoxyuridine was added for 60 hours for all doses of chloramphenicol and to the control. When the tissue culture cells were exposed to increasing doses, increased numbers of sister chromatid exchanges developed. Differences were significantly different to the control.
Arruga
M V
MV
Laboratory of Cytogenetics, Veterinary Faculty, Zaragoza, Spain.
Catalan
J
J
Moreno
C
C
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002701:Chloramphenicol / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D007621:Karyotyping; D008677:Metaphase; D009152:Mutagenicity Tests / Q000662:veterinary; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D002701:Chloramphenicol