title: Nucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline protease of Aspergillus fumigatus.
abstract: An Aspergillus fumigatus extracellular alkaline protease (ALP) which is an enzyme of the subtilisin family is a potential virulent factor of the fungus. The gene encoding ALP was isolated from a genomic library made from DNA of an A. fumigatus isolate. The nucleotide sequence of this gene was compared to that of a cDNA encoding A. oryzae ALP and to that of a cDNA from A. fumigatus encoding the mature ALP protein. Mature A. fumigatus ALP contains 282 amino acids and is encoded by three exons. The pre-proenzyme has a leader sequence of 121 amino acids.
authors.affiliation: Laboratoire de mycologie, Centre hospitalier universitaire vaudois, Lausanne, Switzerland.
authors.forename: K
authors.initials: K
authors.lastname: Jaton-Ogay
authors.forename: M
authors.initials: M
authors.lastname: Suter
authors.forename: R
authors.initials: R
authors.lastname: Crameri
authors.forename: R
authors.initials: R
authors.lastname: Falchetto
authors.forename: A
authors.initials: A
authors.lastname: Fatih
authors.forename: M
authors.initials: M
authors.lastname: Monod
mesh_terms: D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D005091:Exons / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism
chemical_list: D015342:DNA Probes; D012697:Serine Endopeptidases; C032923:microbial serine proteinases