Endothelin-3 stimulates the release of catecholamine from cortical and striatal slices of the rat.
Endothelin-3 (ET-3) evoked the release of dopamine/noradrenaline from cortical slices and dopamine from striatal slices in a concentration-dependent manner. This action peaked slowly and was long-lasting in real-time monitoring, being different from the high K(+)-evoked response. The striatal response to 10 microM of ET-3 was reduced by extracellular Ca2+ depletion to 40% of control and by Ca2+ antagonists, especially nifedipine and flunarizine, to 40% of control. Our findings suggest that ET has a physiological significance in the brain as a neuromodulator for catecholaminergic transmission.
Koizumi
S
S
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Kataoka
Y
Y
Niwa
M
M
Kumakura
K
K
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology*; D003342:Corpus Striatum / Q000187:drug effects / Q000502:physiology*; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D016232:Endothelins / Q000494:pharmacology*; D005071:Evoked Potentials / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D002121:Calcium Channel Blockers; D016232:Endothelins; D004533:Egtazic Acid; D011188:Potassium; D002118:Calcium; D004298:Dopamine; D009638:Norepinephrine