Favourable biospecific reactivity of blood group B antigenic trisaccharide chemically attached to poly-N-(2-hydroxyethyl)acrylamide-coated porous glass.
Blood group B antigenic trisaccharide-beta-aminopropyl glycoside (B-TSAP) covalently attached to poly-N-(2-hydroxyethyl)acrylamide-coated porous glass interacts with anti-B monoclonal antibodies faster than the ligand coupled to CNBr-activated Sepharose 4B and Affi-Gel 10. Rates of hydrophobic adsorption of antibodies on the butyl derivatives of the same supports were measured to evaluate the diffusion input to overall kinetics. The lowest average affinity adsorption time [t1(aff) = 250 s] observed for polymer-coated glass probably arises because of the flexibility of the extended segments of chemisorbed N-substituted polyacrylamide acting as effective spacer arms.
Ivanov
A E
AE
Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
Bovin
N V
NV
Korchagina EYu
Zubov
V P
VP
D000017:ABO Blood-Group System / Q000276:immunology* / Q000378:metabolism; D000180:Acrylic Resins / Q000378:metabolism*; D002846:Chromatography, Affinity; D006386:Hemagglutination Tests; D006801:Humans; D007700:Kinetics; D013379:Substrate Specificity; D014312:Trisaccharides / Q000378:metabolism*
D000017:ABO Blood-Group System; D000180:Acrylic Resins; D014312:Trisaccharides; C074962:poly-N-(2-hydroxyethyl)acrylamide