Rapid and sensitive typing of forensic stains by PCR amplification of polymorphic simple repeat sequences in case work.
After failure of conventional typing and multilocus DNA fingerprinting methods to compare a minute vaginal swab stain with blood from a murder victim and a suspect, we used enzymatic DNA amplification (polymerase chain reaction, PCR) to discriminate the DNAs by typing of simple sequence lengths polymorphisms. A mixed dinucleotide locus in the HLA-DRB gene region and three novel tetranucleotide polymorphisms located autosomally as well as on the human Y chromosome were used to exclude a jailed subject from a case of murder.
Roewer
L
L
Max-Planck-Institut für Psychiatrie, Martinsried, Germany.
Epplen
J T
JT
D000483:Alleles; D001483:Base Sequence; D003075:Coitus; D004247:DNA / Q000032:analysis / Q000097:blood / Q000737:chemistry; D016172:DNA Fingerprinting; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D005787:Gene Frequency; D006684:HLA-DR Antigens / Q000235:genetics; D006708:Homicide; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013094:Spermatozoa / Q000737:chemistry; D014621:Vagina / Q000737:chemistry
D006684:HLA-DR Antigens; D004247:DNA