[Influence of aging and circadian fluctuation of fibrinolytic factors to the responses of these factors during venous occlusion test in healthy subjects].
This study was aimed to clarify the influence of aging and circadian fluctuation of fibrinolytic factors to changes of tissue plasminogen (t-PA) and its inhibitor (PAI-I) during venous occlusion test. Venous occlusion was achieved by application of a sphygmomanometer cuff to the upper arm of elderly healthy subjects (n = 12) and young healthy subjects (n = 12) at 10:00 am and 4:00 pm. Plasma concentration of t-PA, free PAI-1 and total PAI-1 were measured by ELISA. The activity of t-PA was measured by bioimmunoassay using monoclonal antibody for t-PA. Circadian variation was observed in the change of t-PA activity and total PAI-1. These were highly increased in the evening. However, this phenomenon was different between age groups and increase of t-PA activity occurred in elderly subjects, whereas PAI-1 was observed in young subjects. In conclusion, circadian variation and the influence of age should be considered to evaluate results of venous occlusion test.
Motohashi
F
F
2nd Department of Internal Medicine, St. Marianna University School of Medicine, Kawasaki.
Masui
Y
Y
Kamogawa
A
A
Hayashi
T
T
Miyake
F
F
Murayama
M
M
Sugai
J
J
Yasumuro
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D002940:Circadian Rhythm; D004730:Endothelium, Vascular / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008875:Middle Aged; D015849:Plasminogen Inactivators / Q000097:blood*; D010991:Plethysmography; D010959:Tissue Plasminogen Activator / Q000097:blood*; D014680:Veins / Q000502:physiology
D015849:Plasminogen Inactivators; D010959:Tissue Plasminogen Activator