Radioimmunoassay of 5 beta-cholestane-3 alpha,7 alpha,12 alpha,25-tetrol 3-glucuronide in serum of patients with cerebrotendinous xanthomatosis.
A rapid, convenient, and specific radioimmunoassay for 5 beta-cholestane-3 alpha,7 alpha,12 alpha,25-tetrol has been developed. Specific antiserum was obtained from rabbits immunized by the bile alcohol-bovine serum albumin conjugate, which was coupled by an (O-carboxymethyl)oxime bridge at the C-3 position. The assay produces values for serum concentrations of bile alcohol glucuronides in patients with cerebrotendinous xanthomatosis.
Une
M
M
Institute of Pharmaceutical Science, Hiroshima University School of Medicine, Japan.
Kataoka
J
J
Nakata
Y
Y
Hoshita
T
T
D001927:Brain Diseases / Q000097:blood*; D002777:Cholestanols / Q000097:blood*; D006801:Humans; D007457:Iodine Radioisotopes; D009135:Muscular Diseases / Q000097:blood*; D011863:Radioimmunoassay; D013710:Tendons; D014973:Xanthomatosis / Q000097:blood*
D002777:Cholestanols; D007457:Iodine Radioisotopes; C028298:cholestane-3,7,12,25-tetrol-3-glucuronide