title: Studies on the proteins involved in the interaction of high-density lipoprotein with isolated human small intestine epithelial cells.
abstract: Treatment of 125I-labelled high-density lipoprotein ([125I]HDL3) with monospecific polyclonal antibodies against apolipoproteins A-I and A-II resulted in a dose-dependent inhibition of the [125I]HDL3 binding to isolated human small intestine epithelial cells by 25% and 50%, respectively. Both antibodies also inhibited intracellular degradation of [125I]HDL3 by 80%. Treatment of enterocytes with polyclonal antibody against apolipoprotein A-I binding protein, a putative HDL receptor, inhibited both binding and degradation of [125I]HDL3 by these cells by 50%. Antibodies to apolipoprotein A-I, A-II and apo A-I-binding protein also inhibited [125I]HDL3 binding to cholesterol-loaded cells.
authors.affiliation: Institute of Experimental Cardiology, Cardiology Research Center, Moscow, Russia.
authors.forename: D D
authors.initials: DD
authors.lastname: Sviridov
authors.forename: C
authors.initials: C
authors.lastname: Ehnholm
authors.forename: H
authors.initials: H
authors.lastname: Tenkanen
authors.lastname: Pavlov MYu
authors.forename: I G
authors.initials: IG
authors.lastname: Safonova
authors.forename: V S
authors.initials: VS
authors.lastname: Repin
mesh_terms: D000906:Antibodies / Q000276:immunology; D016632:Apolipoprotein A-I / Q000276:immunology / Q000378:metabolism*; D016633:Apolipoprotein A-II / Q000276:immunology / Q000378:metabolism*; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism*; D011257:Pregnancy Proteins; D019998:Racemases and Epimerases
chemical_list: D000906:Antibodies; D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D002352:Carrier Proteins; D008075:Lipoproteins, HDL; D011257:Pregnancy Proteins; D002784:Cholesterol; C052433:NAXE protein, human; D019998:Racemases and Epimerases