Flow cytometry analysis of the expression of neutrophil FMLP receptors.
The number of cell surface FMLP receptors expressed by peripheral blood neutrophils taken from healthy individuals was quantitated using a direct fluorescence label analysed on the FACScan. Receptor numbers, assayed on cells from heparinised blood prepared at 4 degrees C or 37 degrees C or in the presence of LPS (30 ng/ml) were 12,540 +/- SD 2413, 18,588 +/- 4938 and 45,041 +/- 10,744 respectively. Anticoagulation with EGTA resulted in 25-30% greater basal receptor numbers but had no effect on receptor level after maximal stimulation with LPS. Double labelling with a neutrophil-specific mAb showed that the entire neutrophil population expressed FMLP receptors.
Allen
C A
CA
Department of Experimental Medicine, University of Otago Medical School, Dunedin, New Zealand.
Broom
M F
MF
Chadwick
V S
VS
D000911:Antibodies, Monoclonal; D003080:Cold Temperature / Q000009:adverse effects; D004533:Egtazic Acid / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D015870:Gene Expression / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008070:Lipopolysaccharides; D009504:Neutrophils / Q000378:metabolism*; D009842:Oligopeptides; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D009842:Oligopeptides; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D004533:Egtazic Acid; C021642:F-chemotactic peptide; D006493:Heparin; D016650:Fluorescein-5-isothiocyanate