Neuroactive amino acids in the area postrema. An immunocytochemical investigation in rat with some observations in cat and monkey (Macaca fascicularis).
The localization of five neuroactive amino acids in the rat area postrema was studied by postembedding immunocytochemistry in semithin and ultrathin sections. Antisera to GABA, glycine, glutamate and aspartate produced labelling of cells that were identified as neurons in the electron microscope. GABA-like and glycine-like immunoreactivities occurred in about 20% and 60% of the neurons, respectively, and a minor proportion of the cells displayed both immunoreactivities, suggesting a cellular colocalization of GABA and glycine. Immunoreactivities for glutamate and aspartate were found in a large majority of the neurons, including most of the cells that were positive for GABA and/or glycine. Taurine immunoreactivity was highly concentrated in a few small cells with ultrastructural features typical of microglial cells, and in processes that were probably derived from these. Taurine also appeared to be abundant in cells confined to the perivascular space. The electron microscopic, immunogold analysis of the neuropil revealed numerous nerve terminals that were enriched in GABA or glutamate immunoreactivity, compatible with a transmitter role of these amino acids. Glycine immunolabelling was found preferentially in postsynaptic elements, suggesting that the glycine-containing cells lack locally ramifying axon collaterals, and that they mainly project outside the area postrema. Aspartate immunolabelling was also generally low in axon terminals. This is similar to the situation in several other brain areas and could indicate that the latter amino acid primarily serves metabolic functions in the area postrema.
Walberg
F
F
Anatomical Institute, University of Oslo, Norway.
Ottersen
O P
OP
D000818:Animals; D001224:Aspartic Acid / Q000032:analysis*; D001933:Brain Stem / Q000737:chemistry* / Q000648:ultrastructure; D002415:Cats; D005971:Glutamates / Q000032:analysis*; D005998:Glycine / Q000032:analysis*; D007150:Immunohistochemistry; D008252:Macaca fascicularis; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013654:Taurine / Q000032:analysis*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D005971:Glutamates; D013654:Taurine; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; D005998:Glycine