Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction.
A rapid method for the detection of simian immunodeficiency virus (SIV) RNA from peripheral blood mononuclear cells (PBMC) of experimentally infected rhesus macaques by the polymerase chain reaction (PCR) is reported. The PCR was carried out with a complementary DNA (cDNA) template using 3 pairs of primers that were designed to anneal to homologous sequences in conserved regions of 3 molecular clones of SIVmac. The specificity of the primers was confirmed by performing the PCR with template DNA from the 3 molecular clones. SIV-specific RNA was detected from 30 and 50 infected PBMC/6.25 x 10(5) PBMC of two animals.
Blackbourn
D J
DJ
Department of Medical Pharmacology and Toxicology, School of Medicine, University of California, Davis 95616.
Chuang
L F
LF
Sutjipto
S
S
Killam
K F
KF
McCready
P M
PM
Doi
R H
RH
Li
Y
Y
Chuang
R Y
RY
D000818:Animals; D001483:Base Sequence; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000097:blood*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood / Q000235:genetics*; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D013698:Templates, Genetic
D012367:RNA, Viral