H7, a protein kinase C inhibitor, increases the glutathione content of neuroblastoma cells.
It is shown that the intracellular glutathione (GSH) concentration of neuroblastoma-2a cells in culture increases with a maximum at 24 h after starting treatment with 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), an inhibitor of protein kinase C (PKC). Other inhibitors of this and other protein kinases, e.g. sphingosine, staurosporine, and HA 1004, at the concentrations tested, had a less marked or negligible effect on intracellular GSH concentration. 12-O-Tetradecanoylphorbol-13-acetate (TPA) was also tested and showed no significant effect 24 h after addition.
Romero
F J
FJ
Departament de Fisiologia, Universitat de València, Spain.
Llopis
J
J
Felipo
V
V
Miñana
M D
MD
Romá
J
J
Grisolía
S
S
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D004789:Enzyme Activation; D005978:Glutathione / Q000096:biosynthesis*; D007546:Isoquinolines / Q000494:pharmacology*; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013110:Sphingosine / Q000494:pharmacology; D019311:Staurosporine; D013449:Sulfonamides; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000470:Alkaloids; D007546:Isoquinolines; D010879:Piperazines; D013449:Sulfonamides; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D011493:Protein Kinase C; D005978:Glutathione; D019311:Staurosporine; D013110:Sphingosine