Identification, isolation and purification of neurotoxic phospholipases A2 from Vipera russelli venom using polyclonal antibodies.
All the neurotoxic phospholipases A2 present in whole Vipera russelli venom were precipitated selectively from other non-neurotoxic phospholipases A2 and non-phospholipases A2 fractions using antibodies (anti PL-V Ig) raised against one of the purified neurotoxic phospholipases A2 (VRV PL-V). These neurotoxins were identified and isolated in their homogeneous form by chromatographic and electrophoretic methods. The present report of selective isolation and purification of all the neurotoxic phospholipases A2 of V. russelli venom is first of its kind.
Kasturi
S
S
Department of Studies in Biochemistry, University of Mysore, India.
Gowda
T V
TV
D000818:Animals; D000906:Antibodies; D002850:Chromatography, Gel; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009498:Neurotoxins / Q000276:immunology / Q000302:isolation & purification*; D010741:Phospholipases A / Q000276:immunology / Q000302:isolation & purification*; D014757:Viper Venoms / Q000737:chemistry*
D000906:Antibodies; D009498:Neurotoxins; D014757:Viper Venoms; D010741:Phospholipases A