A rapid heat-resistant technique for presumptive identification of Salmonella on desoxycholate-citrate agar.
A caprylate esterase chromogenic test, which was considered to indicate positivity in 5 minutes if a bright colour appeared on the filter paper inoculum site to which one colony had been applied, was used to test 534 Salmonella, 535 other bacteria capable of growth on desoxycholate-citrate agar (DCA), and 517 non-lactose fermenting colonies from stool cultures on DCA. It was found to be 100% sensitive for Salmonella, 99% specific, and more accurate than either direct antiserum agglutination or a urease test in these respects. The test kit could be stored at room temperature.
Dealler
S F
SF
Department of Microbiology, University of Leeds, UK.
Collins
J
J
James
A L
AL
D000362:Agar; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification; D001431:Bacteriological Techniques; D002951:Citrates; D019343:Citric Acid; D015169:Colony Count, Microbial; D003840:Deoxycholic Acid; D004950:Esterases / Q000032:analysis; D006358:Hot Temperature; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D012189:Retrospective Studies; D012475:Salmonella / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D013997:Time Factors
D002951:Citrates; D011933:Reagent Kits, Diagnostic; D003840:Deoxycholic Acid; D019343:Citric Acid; D000362:Agar; D004950:Esterases