title: Subdomains of the rough endoplasmic reticulum in colon goblet cells of the rat: lectin-cytochemical characterization.
abstract: Using lectin binding, we characterized subdomains of the rough endoplasmic reticulum (rER) in goblet cells of the rat colon. In this cell type, special rER regions can be differentiated on the basis of their content of low electron density and dilated cisternal spaces in conventional transmission electron microscopic preparations. The fine fibrillar content of these cisternal regions demonstrated high-affinity binding with lectins from wheat germ, Helix pomatia, Griffonia simplicifolia I-A4 and -B4, and Ricinus communis I, although not with the sialic acid-specific Limax flavus lectin and the fucose-binding Ulex europaeus I lectin. Sugar-inhibitory experiments indicated that glycoconjugates packed within these regions bound the lectins with higher affinity than molecules present in the Golgi apparatus and secretory granules. Furthermore, the lectin binding patterns of the rER subdomains differed from those of the Golgi apparatus and mucin granules: the terminal sugar residues sialic acid and fucose were demonstrable in the Golgi apparatus and mucin granules and were absent from the rER, while galactose-recognizing lectins bound intensely at these rER regions, weakly to Golgi elements, and were almost absent from mucin granules.
authors.affiliation: Institute of Micromorphology and Electronmicroscopy, University of Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Ellinger
authors.forename: M
authors.initials: M
authors.lastname: Pavelka
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D003106:Colon / Q000737:chemistry*; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D006001:Glycoconjugates / Q000032:analysis*; D006056:Golgi Apparatus / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry; D008969:Molecular Sequence Data; D009077:Mucins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006001:Glycoconjugates; D037102:Lectins; D009077:Mucins