title: The intestinal and serum humoral immune response of mice to orally administered antigens in liposomes: II. The response to liposome-entrapped bacterial proteins.
abstract: Effective oral adjuvants are needed to improve the intestinal immune responses to oral vaccines that are based on relatively low molecular weight antigens refined from veterinary pathogens. Liposomes prepared by different methods and composed of phospholipids of varying transition temperature were used to entrap cholera toxin (CT) and fed to mice. No significant increase in the intestinal antibody nor the serum IgA antibody response was detected but levels of serum IgG anti-CT antibody were significantly elevated in the group fed CT in phosphatidylcholine-based liposomes. Levels of antibody were significantly reduced in the groups fed CT in dipalmitoylphosphatidylcholine liposomes. Escherichia coli wall extract (ECWE) entrapped in certain liposome types and fed to mice elicited significantly increased serum anti-ECWE antibody responses but intestinal antibody responses were insignificantly different from the controls. These results suggest that orally administered liposomes fail to act as potent intestinal adjuvants for the entrapped antigens of bacterial origin used in this study.
authors.affiliation: Department of Veterinary Medicine, University of Bristol, Langford, UK.
authors.forename: C J
authors.initials: CJ
authors.lastname: Clarke
authors.forename: C R
authors.initials: CR
authors.lastname: Stokes
mesh_terms: D000276:Adjuvants, Immunologic; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D016667:Bacterial Capsules / Q000276:immunology; D002772:Cholera Toxin / Q000008:administration & dosage / Q000276:immunology*; D004337:Drug Carriers; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D007422:Intestines / Q000276:immunology*; D008081:Liposomes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D004337:Drug Carriers; D007074:Immunoglobulin G; D008081:Liposomes; D002772:Cholera Toxin