Antizyme, a protein induced by polyamines, accelerates the degradation of ornithine decarboxylase in Chinese-hamster ovary-cell extracts.
Ornithine decarboxylase (ODC), the key regulatory enzyme for polyamine biosynthesis, is known to have a short intracellular half-life, and antizyme, an ODC-binding protein induced by polyamines, has been suggested to be involved in the process of ODC degradation. In the present study we demonstrated that antizyme markedly accelerated ATP-dependent degradation of ODC in vitro in an extract from ODC-overproducing Chinese-hamster ovary cells.
Murakami
Y
Y
Department of Nutrition, Jikei University School of Medicine, Tokyo, Japan.
Tanaka
K
K
Matsufuji
S
S
Miyazaki
Y
Y
Hayashi
S
S
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D016466:CHO Cells / Q000201:enzymology*; D006224:Cricetinae; D006863:Hydrogen-Ion Concentration; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*
D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines; D011506:Proteins; C031017:ornithine decarboxylase antizyme; D000255:Adenosine Triphosphate; D009955:Ornithine Decarboxylase