Method to identify specific alleles of a Phanerochaete chrysosporium gene encoding lignin peroxidase.
A method to identify and differentiate allelic variants of the gene encoding lignin peroxidase isozyme H8 is presented. The strategy involves amplifying a variable region of the gene's carboxy terminus by use of the polymerase chain reaction and then probing with allele-specific oligonucleotides.
Gaskell
J
J
Institute for Microbial and Biochemical Technology, USDA Forest Service, Madison, Wisconsin 53705.
Vanden Wymelenberg
A
A
Stewart
P
P
Cullen
D
D
D000483:Alleles; D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology / Q000235:genetics*; D015139:Blotting, Southern; D004271:DNA, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction
D004271:DNA, Fungal; D010544:Peroxidases; C042858:lignin peroxidase