Dodeca satellite: a conserved G+C-rich satellite from the centromeric heterochromatin of Drosophila melanogaster.
To identify sequences from the centromeric region, we have constructed a Drosophila melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA from the minichromosome Dp(1;f)1187 derived from the X chromosome. We describe the structure of one clone isolated in this way. This YAC is structurally unstable and contains tandemly repeated G+C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences are found in the genomes of organisms as distant as Arabidopsis thaliana and Homo sapiens.
Abad
J P
JP
Centro de Biología Molecular (CSIC-UAM), Spain.
Carmena
M
M
Baars
S
S
Saunders
R D
RD
Glover
D M
DM
Ludeña
P
P
Sentis
C
C
Tyler-Smith
C
C
Villasante
A
A
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002503:Centromere / Q000737:chemistry*; D015825:Chromosomes, Fungal; D003596:Cytosine; D004276:DNA, Satellite / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D015723:Gene Library; D016678:Genome; D006147:Guanine; D006570:Heterochromatin / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome
D004276:DNA, Satellite; D006570:Heterochromatin; D006147:Guanine; D003596:Cytosine