title: Nucleic acid-binding properties of the alfalfa mosaic virus movement protein produced in yeast.
abstract: The movement protein of alfalfa mosaic virus (P3) was purified from yeasts transformed with an expression vector containing the P3 gene. Its nucleic acid-binding properties were tested by electrophoretic retardation, nitrocellulose retention, and RNA-protein cross-linking. The recombinant protein had a higher affinity for single-stranded RNA and DNA than for double-stranded nucleic acids. Each nucleic acid molecule bound several protein molecules without sequence specificity. The binding was 80% inhibited by 0.2 M NaCl. These properties are qualitatively similar, but not strictly identical, to those of two other viral movement proteins, the 30-kDa protein of tobacco mosaic virus and the gene I product of cauliflower mosaic virus.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Université Louis Pasteur, Strasbourg, France.
authors.forename: F
authors.initials: F
authors.lastname: Schoumacher
authors.forename: C
authors.initials: C
authors.lastname: Erny
authors.forename: A
authors.initials: A
authors.lastname: Berna
authors.forename: T
authors.initials: T
authors.lastname: Godefroy-Colburn
authors.forename: C
authors.initials: C
authors.lastname: Stussi-Garaud
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D000455:Medicago sativa; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000502:physiology*; D011485:Protein Binding; D012367:RNA, Viral / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D012367:RNA, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins