Analysis of antibody responses directed against two Onchocerca volvulus antigens defined by monoclonal antibodies.
Monoclonal antibodies, reactive with antigens solubilised from the body wall of intact female Onchocerca volvulus using 2% 2-beta-mercaptoethanol, have been characterised. Two IgG1 antibodies, Cam1 and Cam28, recognised antigens of apparent molecular weights of 18,000 and 28,000; and 120,000, respectively. The target antigens of Cam1 and Cam28 could be localised in the cuticle. Inhibition ELISAs showed that target epitopes of both monoclonal antibodies induce an antibody response in onchocerciasis patients. 153 sera from Sierra Leonean patients were tested for their individual antibody levels against antigen epitopes recognised by Cam1 and Cam28. Patients within the age of 5-8 years had the highest levels of antibodies against the Cam28-epitope, whereas patients above 60 years had almost none. Amicrofilaremic patients had higher anti-Cam28 antibody levels than microfilaremic patients and there was a significant difference between groups with no chronic skin disease and those with mild or severe signs. A high percentage of patients (80.4%) recognised the Cam1-epitope, highest antibody levels being found in patients within the age group of 15 to 45 years and in microfilaremic patients. However, levels of antibodies inhibiting monoclonal antibody Cam1 could not be correlated with presence or absence of skin disease.
Engelbrecht
F
F
Department of Pathology, University of Cambridge, UK.
Eisenhardt
G
G
Turner
J
J
Sundaralingam
J
J
Owen
D
D
Braun
G
G
Connor
V
V
Taylor
D W
DW
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology*; D001667:Binding, Competitive; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D006825:Hybridomas; D007118:Immunoassay; D007136:Immunoglobulins / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009854:Onchocerca / Q000276:immunology*; D009855:Onchocerciasis / Q000175:diagnosis / Q000276:immunology*; D012876:Skin Diseases, Parasitic / Q000175:diagnosis / Q000276:immunology; D013045:Species Specificity
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007136:Immunoglobulins