[Determination of m-nifedipine and its pharmacokinetic study in rabbits by high-pressure liquid chromatography].
A high-pressure liquid chromatographic method was developed for determination of m-nifedipine in plasma using a chemical bonded C-18 phase column (YWG-C18 10 microns, made in China) with nitrendipine as internal standard. To increase life of the YWG-C18 column a mixture of methanol and 5 mmol.L-1 phosphate buffer (70:30 vol/vol) was selected as mobile phase with a flow rate of 0.8 ml.min-1. The method was sensitive to m-nifedipine 3 ng.ml-1 plasma and the standard curve was linear from 10 to 1000 ng.ml-1 with correlation coefficient of 0.99. The within-day and day-to-day precisions (CV) of this method were 4.5% and 7.0%, respectively, with recoveries of 95-102% (10-1000 ng.ml-1). There was no interference with nifedipine, amiodarone, propranol, and verapamil. A pharmacokinetic study on m-nifedipine was carried out in 8 rabbits. A better computer fitted to a two-compartment model was observed using 3P87 program. The parameters obtained were as follow: Vc 6.3 L.kg-1, Cl 0.021 L.kg-1.min-1, T1/2 alpha 30 min, T1/2 beta 230 min, AUC 102 micrograms.min.ml-1.
Liang
Y B
YB
Institute of Clinical Pharmacology, Xi'an Medical University, China.
Ma
X Y
XY
Wu
G J
GJ
Wang
L F
LF
Liu
D K
DK
Xing
J F
JF
Zhao
G S
GS
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008297:Male; D009543:Nifedipine / Q000097:blood / Q000493:pharmacokinetics*; D011817:Rabbits
D009543:Nifedipine