Association of blebbing with assembly of cytoskeletal proteins in ATP-depleted EL-4 ascites tumour cells.
ATP depletion in EL-4 ascites tumour cells rapidly induced the changes in cell morphology (blebbing), cytoskeletal protein assembly and finally resulted in cell death. After 1 hr of incubation with 2 microM rotenone (inhibitor of respiration) in glucose-free medium, when ATP level was 4% of the initial level, there were increases in triton-insoluble actin and vinculin levels (2.5-fold and 2.8-fold, respectively) and 44% of cells showed blebs; such treatment damaged cells irreversibly. Ca2+ removal did not diminish the effect of ATP depletion on cytoskeleton, blebbing and cell death, although the elevation of free intracellular Ca2+ in rotenone-treated cells was prevented. The role of ATP in maintaining cytoskeleton and cell shape is discussed.
Gabai
V L
VL
Institute of Medical Radiology, Academy of Medical Sciences of the USSR, Obninsk.
Kabakov
A E
AE
Mosin
A F
AF
D019297:2,4-Dinitrophenol; D000255:Adenosine Triphosphate / Q000502:physiology*; D002118:Calcium / Q000032:analysis; D016923:Cell Death / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000032:analysis*; D004140:Dinitrophenols / Q000494:pharmacology; D004533:Egtazic Acid; D015151:Immunoblotting; D009840:Oligomycins / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000648:ultrastructure
D003598:Cytoskeletal Proteins; D004140:Dinitrophenols; D009840:Oligomycins; D012402:Rotenone; D004533:Egtazic Acid; D000255:Adenosine Triphosphate; D019297:2,4-Dinitrophenol; D002118:Calcium