Extraction and characterisation of the intact form of bovine vitreous type IX collagen.
We provide the first biochemical characterisation of intact type IX collagen extracted from bovine vitreous. It possesses a shortened alpha 1(IX) chain (M(r) 64K) compared to its cartilage counterpart (M(r) 84K). All the vitreous type IX collagen is in a proteoglycan form, its glycosaminoglycan constituent being a chondroitin/dermatan sulphate component of M(r) 15-60K attached to the alpha 2(IX) chain. This contrasts with previous findings in chick vitreous where a very long glycosaminoglycan chain of M(r) approximately 350K was demonstrated.
Bishop
P
P
Department of Biochemistry & Molecular Biology, University of Manchester, U.K.
McLeod
D
D
Ayad
S
S
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002500:Centrifugation, Isopycnic; D002809:Chondroitin Sulfates / Q000737:chemistry; D003094:Collagen / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D003871:Dermatan Sulfate / Q000737:chemistry; D011509:Proteoglycans / Q000737:chemistry*; D014822:Vitreous Body / Q000737:chemistry* / Q000276:immunology
D011509:Proteoglycans; D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates; D003094:Collagen