The use of osmicated tissues for Lowicryl K4M embedding.
Tissue chopper slices of rat incisor, rat parotid gland, and chicken tibiae, fixed with 1% glutaraldehyde, were post-fixed with potassium ferrocyanide-reduced osmium tetroxide, dehydrated with methanol, and conventionally embedded in Lowicryl K4M at -20 degrees C. The tissues showed an ultrastructural appearance comparable with that of their Epon-embedded counterparts and, in particular, the Golgi apparatus was well defined. Furthermore, Lowicryl K4M-embedded osmicated tissues permitted both post-embedding lectin-gold cytochemistry and immunogold labeling.
Nanci
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A
Faculty of Dentistry, Université de Montréal, Québec, Canada.
Mazariegos
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Fortin
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D000180:Acrylic Resins; D000818:Animals; D002645:Chickens; D005404:Fixatives; D006046:Gold; D007150:Immunohistochemistry; D037102:Lectins; D008297:Male; D016253:Microscopy, Immunoelectron; D009992:Osmium / Q000737:chemistry*; D051381:Rats; D011919:Rats, Inbred Strains; D016707:Tissue Fixation
D000180:Acrylic Resins; D005404:Fixatives; D037102:Lectins; D009992:Osmium; D006046:Gold; C037883:Lowicryl 4KM resin