Noninvasive liver-iron quantification by computed tomography in iron-overloaded rats.
OBJECTIVE
The benefit of computed tomography (CT) for the noninvasive determination of liver-iron concentration in human iron-overload diseases is a controversy in the literature. To study the sensitivity of CT for liver-iron quantification under experimental conditions, the authors measured single- and dual-energy CT numbers in vivo in the livers of iron-overloaded rats.


METHODS
Thirty-five rats were subjected to an iron-rich diet for various periods, from 1 to 20 weeks, then scanned by single- and dual-energy CT. CT absorption was correlated to liver-iron content, which was determined by wet ashing and spectrophotometry.


RESULTS
Whereas a good correlation (r = 0.99 at 96 kV; r = 0.95 at 125 kV) between CT numbers and liver-iron concentration was found, CT was insensitive to low concentrations of iron. Dual-energy CT scanning results showed greater scattering in liver-iron quantification compared with single-energy CT.


CONCLUSIONS
In rats, the sensitivity of single- and dual-energy CT is too low to quantify liver iron in the diagnostically most relevant region of mild liver siderosis (1-3 mg iron/g wet weight [w.wt]).
Nielsen
P
P
Abteilung Medizinische Biochemie, Universitätskrankenhaus Eppendorf, Hamburg, Germany.
Engelhardt
R
R
Fischer
R
R
Heinrich
H C
HC
Langkowski
J H
JH
Bücheler
E
E
D000818:Animals; D005260:Female; D007501:Iron / Q000032:analysis / Q000633:toxicity*; D008099:Liver / Q000737:chemistry* / Q000000981:diagnostic imaging; D051381:Rats; D011919:Rats, Inbred Strains; D014057:Tomography, X-Ray Computed
D007501:Iron