twine, a cdc25 homolog that functions in the male and female germline of Drosophila.
twine is the second homolog of the fission yeast gene cdc25 to be found in Drosophila. Both string and twine cDNAs can rescue a temperature-sensitive cdc25 mutation in fission yeast, but not a deletion. We detect the expression of string but not twine transcripts in the proliferating cells of newly cellularized embryos, in third instar larval brains, and in imaginal discs. Both genes are abundantly expressed in nurse cells during oogenesis, the maternal transcripts persisting throughout the syncytial stage of embryonic development. In the testis, twine transcripts are seen in the growing stage of premeiotic cysts. Analysis of a twine mutant suggests a requirement for the gene during oogenesis, during syncytial embryonic development, and for male meiosis. Meiosis does not occur in homozygous twine males, which produce cysts containing 16 rather than 64 spermatids.
Alphey
L
L
Department of Biochemistry, The University, Dundee, Scotland.
Jimenez
J
J
White-Cooper
H
H
Dawson
I
I
Nurse
P
P
Glover
D M
DM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D002455:Cell Division; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004247:DNA / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D005796:Genes; D005816:Genetic Complementation Test; D007246:Infertility / Q000235:genetics; D007301:Insect Hormones / Q000235:genetics; D019476:Insect Proteins; D008297:Male; D008540:Meiosis; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D020666:ras-GRF1
D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D011506:Proteins; D012333:RNA, Messenger; D020666:ras-GRF1; C075186:twe protein, Drosophila; D004247:DNA; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; C059217:stg protein, Drosophila