title: Carotid chemoreceptors, systemic blood pressure, and chronic episodic hypoxia mimicking sleep apnea.
abstract: We have described a rat model that responds to repetitive episodic hypoxia (12-s infusions of nitrogen into daytime sleeping chambers every 30 s, 7 h/day for 35 days) with an increase in diurnal systemic blood pressure. We hypothesized that afferent information from the peripheral chemoreceptors may be necessary to produce diurnal blood pressure elevation in this hypoxia model. Carotid body denervation (CBD) was accomplished by severing both carotid sinus nerves in two groups of male Wistar rats (250-375 g). Group 4 CBD rats were subjected to intermittent hypoxia for 35 days (3-5% nadir ambient O2) as described above, whereas group 5 CBD rats remained unhandled in their usual cages. Additional sham-operated controls included group 2 sham-"hypoxia" rats, which were housed in chambers identical to the hypoxia rats but supplied with compressed air instead of nitrogen, group 1 (not denervated) rats, which remained unhandled in their usual cages, and group 3 sham-operated rats, which were subjected to 35 days of intermittent hypoxia identical to group 4 CBD rats. Femoral arterial baseline and end-of-study blood pressures were measured in conscious rats. The group 3 rats exposed to episodic hypoxia displayed a 13-mmHg increase in mean blood pressure, whereas the other groups showed no significant change from baseline. Left ventricular hypertrophy was evident in all rats exposed to episodic hypoxia, but right ventricular hypertrophy was evident only in the group 4 rats. All CBD rats developed increased hematocrit and hemoglobin, while the group 3 rats (non-CBD, episodic hypoxia) did not. The baroreceptor reflex at baseline was not depressed in the CBD rats.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pharmacology, Ruprecht-Karls University, Heidelberg, Federal Republic of Germany.
authors.forename: E C
authors.initials: EC
authors.lastname: Fletcher
authors.forename: J
authors.initials: J
authors.lastname: Lesske
authors.forename: R
authors.initials: R
authors.lastname: Behm
authors.forename: C C
authors.initials: CC
authors.lastname: Miller
authors.forename: H
authors.initials: H
authors.lastname: Stauss
authors.forename: T
authors.initials: T
authors.lastname: Unger
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002344:Carotid Body / Q000503:physiopathology; D002628:Chemoreceptor Cells / Q000503:physiopathology; D003714:Denervation; D004195:Disease Models, Animal; D000860:Hypoxia / Q000503:physiopathology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012891:Sleep Apnea Syndromes / Q000503:physiopathology*