A semiautomated flow injection procedure for acetylcholinesterase and cholinesterase activities.
A stopped-flow FIA adaptation of the classical Ellman's colorimetric procedure for the measurement of acetylcholinesterase or cholinesterase activity is described. The samples are injected into a flow analytical system which is provided with an electronic timer and an electrovalve in order to stop the flow when the sample is inside the measurement cell. In this way, the absorbance-time relationship is obtained with a savings of sample, time, and reagents.
López García
I
I
Department of Analytical Chemistry, University of Murcia, Spain.
Ruiz Abellán
F
F
Hernández Córdoba
M
M
D000110:Acetylcholinesterase / Q000097:blood*; D000122:Acetylthiocholine / Q000737:chemistry; D002091:Butyrylcholinesterase / Q000097:blood*; D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D013057:Spectrum Analysis; D013696:Temperature
D007202:Indicators and Reagents; D000122:Acetylthiocholine; D004228:Dithionitrobenzoic Acid; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase