The rough (ro+) gene as a dominant P-element marker in germ line transformation of Drosophila melanogaster.
We describe a new vector for the P-element-mediated introduction of gene constructs into the germ line of Drosophila melanogaster. The P-element vector carries 6.8 kb of genomic DNA containing the rough gene (ro) from D. melanogaster and a polylinker (MCS) containing ten unique cloning sites. To demonstrate its utility, we have cloned into the MCS of this vector, the firefly luciferase (Luc)-encoding gene (luc) under the control of the D. melanogaster hsp70 promoter and have transformed flies with the resultant P-element. Single insertions of this element, whether in the hemizygous or homozygous condition, completely rescued the ro- mutation and directed heat-inducible synthesis of Luc mRNA and enzyme.
Lockett
T J
TJ
CSIRO, Division of Biomolecular Engineering, North Ryde, NSW, Australia.
Lewy
D
D
Holmes
P
P
Medveczky
K
K
Saint
R
R
D000818:Animals; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005799:Genes, Dominant; D005819:Genetic Markers; D005822:Genetic Vectors; D008297:Male; D008869:Microtubule-Associated Proteins; D010957:Plasmids; D015183:Restriction Mapping; D014157:Transcription Factors / Q000235:genetics*; D014170:Transformation, Genetic
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005819:Genetic Markers; D008869:Microtubule-Associated Proteins; C064993:ROD protein, Drosophila; D014157:Transcription Factors