Ribosomal DNA sequences in the differentiation of pathogenic and non-pathogenic isolates of Entamoeba histolytica.
Recombinant ribosomal DNA sequences were amplified by PCR and used as probes to perform a fingerprint analysis of total DNA from different Entamoeba histolytica isolates. RFLPs obtained with one of the probes, R-1, support previous proposals that pathogenic and non-pathogenic E. histolytica are closely related, yet genotypically distinct. Another probe, R-2, while not distinguishing between the two forms of E. hystolytica, was able to differentiate between them and E. moshkovskii, which has morphologically identical cysts and trophozoites. A third probe, BR-1, identified strain-specific RFLPs.
Cruz-Reyes
J A
JA
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine.
Spice
W M
WM
Rehman
T
T
Gisborne
E
E
Ackers
J P
JP
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D016172:DNA Fingerprinting; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis* / Q000737:chemistry; D004274:DNA, Recombinant; D004275:DNA, Ribosomal; D004748:Entamoeba histolytica / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D009693:Nucleic Acid Hybridization; D016053:RNA, Protozoan / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D015342:DNA Probes; D016054:DNA, Protozoan; D004274:DNA, Recombinant; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012335:RNA, Ribosomal