Electron-capture gas chromatographic procedure for simultaneous determination of amphetamine and N-methylamphetamine.
An electron-capture gas chromatographic procedure for the simultaneous determination of amphetamine and N-methylamphetamine in biological samples is described. The method employs extraction with the ion-pairing reagent bis(2-ethylhexyl)phosphoric acid, and back-extraction with 0.5 M hydrochloric acid. The hydrochloric acid phase is basified, and the amphetamines and the internal standard benzylamine are derivatized with pentafluorobenzenesulfonyl chloride prior to analysis on a gas chromatograph equipped with a capillary column. Levels of amphetamine and N-methylamphetamine have been determined in the urine and liver of rats treated chronically with (-)-deprenyl.
Paetsch
P R
PR
Department of Psychiatry, University of Alberta, Edmonton, Canada.
Baker
G B
GB
Caffaro
L E
LE
Greenshaw
A J
AJ
Rauw
G A
GA
Coutts
R T
RT
D000661:Amphetamine / Q000378:metabolism* / Q000652:urine; D000818:Animals; D002849:Chromatography, Gas; D004583:Electrons; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000737:chemistry*; D008297:Male; D008694:Methamphetamine / Q000378:metabolism* / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D012642:Selegiline / Q000008:administration & dosage
D012642:Selegiline; D008694:Methamphetamine; D000661:Amphetamine