[A method for studying the three-dimensional structure of organs and tissue in pathology].
A new technique of scanning electron microscopy for visualization of hidden cellular surfaces in organs and tissue has been developed. It employs the sample incubation in 30% potassium hydroxide and 96% alcohol mixed in equal amounts and dissociation until single cells appear in the solution, this being checked under phase-contrast microscope. The method is beneficial in non-standard cases of three-dimensional organization of organs and tissues.
Rekhter
M D
MD
Kolpakov
V A
VA
Mironov
A A
AA
D000818:Animals; D002477:Cells / Q000648:ultrastructure*; D000431:Ethanol; D006801:Humans; D006878:Hydroxides; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008858:Microscopy, Phase-Contrast; D011188:Potassium; D017680:Potassium Compounds
D006878:Hydroxides; D017680:Potassium Compounds; D000431:Ethanol; D011188:Potassium; C029943:potassium hydroxide
Metod issledovaniia trekhmernoĭ struktury organov i tkaneĭ v patologii.