Results of the DGTI workshop on the evaluation of the reactivity of monoclonal anti-D.
All D+ samples were detected by all monoclonal antibodies without problems. Very few of the 3000 D- samples showed a +/- to ++ reaction with some of the IgG type mabs using the Coombs technique. It is still open whether these are specific reactions with weak positive samples, which were negative with polyclonal sera. IgM type mabs detected up to 100% of the Du samples dependent on the Rh-phenotype and the technique used (CCDuee greater than CcDuee greater than ccDuEe). Most of the CCDuee samples reacted very strong and should no longer be regarded as Du.
Sonneborn
H H
HH
Biotest AG, Research Dept, Dreieich, FRG.
Darda
C
C
Ernst
M
M
Flegel
W A
WA
Helmbold
W
W
Kasulke
D
D
Kühnl
P
P
Mueller-Eckhardt
C
C
Northoff
H
H
Poschmann
A
A
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001803:Blood Transfusion; D006801:Humans; D007518:Isoantibodies / Q000235:genetics*; D010641:Phenotype
D000911:Antibodies, Monoclonal; D007518:Isoantibodies; C033242:RH-antibodies