Ex vivo versus in situ resection of segmental liver grafts in pigs--a comparison in immediate and four-hour-stored grafts.
This study compared the function of reduced grafts prepared in situ or ex vivo and transplanted immediately or after 4 hr of cold storage. Measurements of acid/base balance, plasma electrolytes, albumin, and urea showed no differences between groups. There was no difference between the increase and decline of plasma AST in recipients of grafts transplanted immediately after either ex vivo or in situ reduction; the increase in plasma AST of recipients of stored grafts was up to 10-fold and persisted until the end of the study at 7 days, with some decline. Plasma fibrinogen decreased intraoperatively but levels were restored within 24 hr in all groups; plasma prothrombin and partial thromboplastin times were not significantly disturbed. The patterns of decline and return of tissue adenine nucleotides were similar in all groups. While the regenerative response measured by tissue thymidine kinase and mitotic figures was not different between the groups, comparison with results from a group of partially hepatectomized animals showed a 3-4-fold depression in response in reduced liver grafts. The contributions of the effects of ischemia, flushing, and preservation to the depressed regenerative response of reduced liver grafts need to be determined. The present studies suggest however, that with regard to functional assessment, results are not affected either by ex vivo or in situ reduction of the graft, or by cold storage for 4 hr.
Roncone
A
A
Department of Surgery, University of Cape Town, South Africa.
Pienaar
H
H
Mahlati
G
G
Rose-Innes
C
C
McLeod
H
H
Kahn
M B
MB
Hickman
R
R
D000227:Adenine Nucleotides / Q000032:analysis; D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001777:Blood Coagulation / Q000502:physiology; D015925:Cryopreservation; D005978:Glutathione; D006085:Graft Survival; D007328:Insulin; D007511:Ischemia / Q000378:metabolism; D008099:Liver / Q000098:blood supply / Q000737:chemistry / Q000378:metabolism; D008115:Liver Regeneration; D016031:Liver Transplantation / Q000502:physiology*; D019188:Organ Preservation Solutions; D011887:Raffinose; D012996:Solutions; D013552:Swine; D013997:Time Factors
D000227:Adenine Nucleotides; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose