An in situ enamel section model for assessment of enamel re/demineralization potential.
An enamel-section-carrying intra-oral appliance to predict the results of double-blind anti-caries studies has been developed. Initial validation was against the F concentration effect attained in a clinical trial where three sodium monofluorophosphate (SMFP) dentifrices were used. Original appliance-based work showed significant differences in remineralization between non-F and F dentifrices, but not between different F dentifrices. However, it was shown later that acidified gel-prepared lesions were not as responsive as solution-prepared lesions to de- and remineralizing processes, and lesion remineralization rates were found to be dependent on initial lesion size. An in situ cross-over study was then repeated with use of acid-solution-created lesions, and seven volunteers completed the project. Each brushed twice daily x 2 min with either 0, 1000, or 2500 ppm F, as SMFP dentifrice. After a two-week wash-out, subjects wore the appliances for four weeks. Enamel mineral content was assessed at 0, two, and four weeks via microradiography/microdensitometry, and a statistically significant dose-response was obtained between non-F and F as well as between 1000 and 2500 ppm F pastes, i.e., as per the three-year clinical trial data. Hence, the model's suitability for pre-clinical screening was confirmed. It has also been used in caries microbiological studies, in root caries investigations, and currently in chewing gum cariogenicity experiments.
Stephen
K W
KW
Department of Oral Medicine and Pathology, University of Glasgow Dental Hospital and School, Scotland.
Damato
F A
FA
Strang
R
R
D003743:Dental Enamel / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D003773:Dental Plaque / Q000382:microbiology; D004305:Dose-Response Relationship, Drug; D004867:Equipment Design; D005459:Fluorides / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D008852:Microradiography; D008903:Minerals / Q000032:analysis; D008954:Models, Biological; D017001:Tooth Demineralization / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014089:Tooth Remineralization
D008903:Minerals; D005459:Fluorides