title: Immunolocalization of laminin, heparan-sulfate proteoglycan, entactin and type IV collagen in the rat anterior pituitary. II. An in vitro study on primary cultures.
abstract: The distribution of four basement membrane components: laminin (LAM), type IV collagen (Coll. IV), heparan-sulfate proteoglycan (HSPG), and entactin (ENT), was studied by immunocytochemistry in primary cultures of adult rat anterior pituitaries. In such cultures, the pituitary cells are deprived of their normal environment of adjacent cells and basement membranes (BM), and of the connectivo-vascular system of the hypophysis. In this dissociated system, pituitary cells grow as small clusters upon a monolayer of fibroblasts. LAM was found highly expressed in endocrine and in folliculo-stellate cells. Very small amounts of Coll. IV, but neither HSPG nor ENT, could be detected in endocrine cells. In contrast, in fibroblasts, very large amounts of Coll. IV, HSPG, and ENT, and a lower quantity of LAM were detected. At the ultrastructural level, the immunoreactive components present within the cells were located in the subcellular compartments involved in the elaboration of exported products. In addition to that intracellular distribution, the four constituents were observed in an extracellular matrix which appeared between the cultured cells, either as an amorphous material, or as a more or less dense reticular network, weakly stained with anti-LAM, but strongly stained with the other antibodies. Thus, the present immunocytochemical data support the implication of pituitary endocrine cells, at least for LAM secretion, in the elaboration of a novel extracellular matrix in primary cultures. In addition, a cooperation with non-endocrine cells seemed to be required for the production of the four BM components.
authors.affiliation: Groupe de Neuroendocrinologie Cellulaire et Moléculaire, Collège de France, Paris.
authors.forename: E
authors.initials: E
authors.lastname: Vila-Porcile
authors.forename: R
authors.initials: R
authors.lastname: Picart
authors.forename: M
authors.initials: M
authors.lastname: Vigny
authors.forename: A
authors.initials: A
authors.lastname: Tixier-Vidal
authors.forename: C
authors.initials: C
authors.lastname: Tougard
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis*; D005109:Extracellular Matrix / Q000737:chemistry / Q000648:ultrastructure; D006497:Heparan Sulfate / Q000032:analysis*; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior / Q000737:chemistry* / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003094:Collagen; D006497:Heparan Sulfate; D007797:Laminin; D008562:Membrane Glycoproteins; C029967:nidogen