The quantitation of long-chain acyl-CoA in mammalian tissue.
Intracellular long-chain acyl-CoA esters are key metabolites in lipid metabolism. A rapid procedure was developed for the isolation of long-chain acyl-CoA from mammalian tissues. Acyl-CoA was extracted from the tissue with chloroform/methanol and separated from other lipid-containing metabolites by phase partition with solvents. The content and the molecular species of acyl-CoA were determined by gas-liquid chromatography. In rat liver and hamster heart, the total acyl-CoA content was estimated to be 83 +/- 11 and 61 +/- 9 nmol/g wet weight, respectively. The results obtained are comparable to those reported in previous studies. The relative ease of this procedure would permit the determination of acyl-CoA contents in a large number of samples.
Tardi
P G
PG
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Mukherjee
J J
JJ
Choy
P C
PC
D000214:Acyl Coenzyme A / Q000032:analysis*; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D006224:Cricetinae; D005227:Fatty Acids / Q000032:analysis; D008099:Liver / Q000737:chemistry*; D008297:Male; D008647:Mesocricetus; D051381:Rats
D000214:Acyl Coenzyme A; D005227:Fatty Acids