Amitriptyline and amitriptyline metabolites in blood and cerebrospinal fluid following human overdose.
The toxicokinetics of amitriptyline were studied in nine patients admitted to hospital in Matthew-Lawson Coma Scale grade III-IV after an estimated ingestion of 1-5 g amitriptyline. Gastric lavage was performed and 50 g activated charcoal were given orally. Venous blood samples were taken on admission and at 1, 2, 4, 8, and 24 h, and in some patients at 36 and 48 h after admission. Arterial blood samples were taken at 1, 4, 8, and 24 h after admission. Lumbar punctures were performed 1 h after admission in 8 patients and again 4 h later in 5 patients. A urine sample was screened for other drugs. The bound and unbound fraction of amitriptyline and its metabolites nortriptyline, E and Z forms of 10-OH-amitriptyline and nortriptyline were analyzed in plasma, whole blood, red blood cells, and cerebrospinal fluid using an HPLC technique. The T1/2 alpha and T1/2 beta for amitriptyline were 1.5 - 3.1 and 15 - 43 h respectively. The rate of elimination of amitriptyline was not dose-dependent. The arteriovenous differences in the total amitriptyline+nortriptyline concentration were maximal in patients admitted soon after intake of drugs. Amitriptyline concentrations in cerebrospinal fluid were quantitatively similar to the unbound amitriptyline concentration in blood. The highest cerebrospinal fluid amitriptyline concentration was 506 nmol/L. There were large individual differences in plasma, blood and cerebrospinal fluid concentrations between different individuals. Repeated quantitative analysis of amitriptyline and its metabolites is unlikely to contribute to the clinical management of most patients with amitriptyline overdose.
Hultén
B A
BA
Department of Anaesthesia and Intensive Care, Salhgrens Hospital, Gothenburg, Sweden.
Heath
A
A
Knudsen
K
K
Nyberg
G
G
Svensson
C
C
Mårtensson
E
E
D000328:Adult; D000639:Amitriptyline / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D062787:Drug Overdose; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006207:Half-Life; D006801:Humans; D008297:Male; D008875:Middle Aged; D009661:Nortriptyline / Q000378:metabolism; D012680:Sensitivity and Specificity
D000639:Amitriptyline; D009661:Nortriptyline