Specific carbon-13 labelling of leucine residues in human growth hormone.
Biosynthetic human growth hormone specifically 13C-labelled in the carbonyl positions of all 26 leucine residues has been obtained by recombinant DNA techniques using 13C-labelled leucine and an E. coli strain that requires leucine. It is shown that, on the whole, the labelling is specific with no significant mislabelling as would have been the case had the 13C-labelled leucine been metabolized.
Christensen
T
T
Novo Nordisk A/S, Bagsvaerd, Denmark.
Jensen
E B
EB
Junker
F
F
Dalbøge
H
H
Abildgaard
F
F
Led
J J
JJ
D002247:Carbon Isotopes; D004926:Escherichia coli / Q000378:metabolism; D013006:Growth Hormone / Q000096:biosynthesis / Q000737:chemistry*; D006801:Humans; D007930:Leucine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry
D002247:Carbon Isotopes; D011994:Recombinant Proteins; D013006:Growth Hormone; D007930:Leucine