Assembly and processing of subunit II (PsaD) precursor in the isolated photosystem-I complex.
The precursor of photosystem I (PSI) subunit II (pre-subunit II) synthesized in vitro, was found to bind to the holo-PSI complex, both within the thylakoids and outside, after detergent extraction of PSI from the membranes. Chloroplast stromal fraction added to the purified PSI complexes, containing the labeled pre-subunit II, induced the processing of the precursor to the mature form. This implies that processing can occur within the isolated complex, after the integration of the precursor. The results presented suggest that certain aspects of biogenesis of membranal protein complexes can be studied in detergent-extracted purified complexes.
Cohen
Y
Y
Department of Botany, Hebrew University of Jerusalem, Israel.
Nechushtai
R
R
D004591:Electrophoresis, Polyacrylamide Gel; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational
D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D011498:Protein Precursors; C070290:PsaD protein, cyanobacteria