title: Helicobacter pylori lipopolysaccharide effect on the synthesis and secretion of gastric sulfomucin.
abstract: The effect of H. pylori lipopolysaccharide on the synthesis and secretion of sulfated mucin in gastric mucosa was investigated using mucosal segments incubated in the presence of [3H]proline, [3H]glucosamine and [35S]Na2SO4. The lipopolysaccharide, while showing no discernible effect on the apomucin synthesis was found to inhibit the process of mucin glycosylation and sulfation, which at 100 micrograms/ml lipopolysaccharide reached the optimal inhibition of 65%. The analysis of mucin secretory responses revealed that the lipopolysaccharide by first 15 min caused a 57% stimulation in sulfomucin secretion followed thereafter by inhibition, which reached maximum of 32% by 45 min. The results suggest that colonization of gastric mucosa by H. pylori may be detrimental to the process of gastric sulfomucin synthesis and secretion.
authors.affiliation: Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103.
authors.forename: Y H
authors.initials: YH
authors.lastname: Liau
authors.forename: R A
authors.initials: RA
authors.lastname: Lopez
authors.forename: A
authors.initials: A
authors.lastname: Slomiany
authors.forename: B L
authors.initials: BL
authors.lastname: Slomiany
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism; D006031:Glycosylation; D016480:Helicobacter pylori; D007700:Kinetics; D008070:Lipopolysaccharides; D009077:Mucins / Q000096:biosynthesis* / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D051381:Rats; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014316:Tritium
chemical_list: D008070:Lipopolysaccharides; D009077:Mucins; D013431:Sulfates; D013462:Sulfur Radioisotopes; C012036:sodium sulfate; D014316:Tritium; D011392:Proline; D005944:Glucosamine