Interferon-alpha and dexamethasone inhibit adhesion of T cells to endothelial cells and synovial cells.
We investigated whether interferon-gamma (IFN-gamma), interferon-alpha (IFN-alpha) and glucocorticoids affected the adhesion of T cells to human umbilical endothelial cells or human synovial cells. About 30% of peripheral blood T cells could bind to unstimulated endothelial cells, but only a few T cells could bind to unstimulated synovial cells. When both endothelial cells and synovial cells were cultured with recombinant IFN-gamma (rIFN-gamma), the percentage of T cell binding to both types of cells increased in a dose-dependent manner. rIFN-alpha and dexamethasone blocked the T cell binding to unstimulated endothelial cells. Furthermore, rIFN-alpha and dexamethasone suppressed T cell binding to both endothelial cells and synovial cells stimulated by IFN-gamma, and also inhibited intercellular adhesion molecule-1 (ICAM-1) expression on both endothelial cells and synovial cells stimulated by IFN-gamma. These results suggest that IFN-alpha and glucocorticoids may inhibit T cell binding to endothelial cells or synovial cells by modulating adhesion molecule expression on these cells.
Eguchi
K
K
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Kawakami
A
A
Nakashima
M
M
Ida
H
H
Sakito
S
S
Matsuoka
N
N
Terada
K
K
Sakai
M
M
Kawabe
Y
Y
Fukuda
T
T
D001172:Arthritis, Rheumatoid / Q000503:physiopathology; D002448:Cell Adhesion / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology; D010003:Osteoarthritis / Q000503:physiopathology; D011994:Recombinant Proteins / Q000494:pharmacology; D013583:Synovial Membrane / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology*; D013997:Time Factors; D014470:Umbilical Cord / Q000502:physiology*
D016898:Interferon-alpha; D011994:Recombinant Proteins; D003907:Dexamethasone; D007371:Interferon-gamma