Epidermal growth factor induces glucose transport in primary cell cultures derived from human astrocytic glioma biopsies.
The gene for the epidermal growth factor (EGF) receptor is amplified in a variety of neoplastic tissues, including malignant gliomas. To reveal whether increased sensitivity to EGF has significance for the supply of metabolic substrate to tumor cells, the rate of glucose transport was determined in cells exposed to EGF for up to six hours. In the epidermoid carcinoma line A431, and in primary cultures from 7/12 human glioma biopsies, EGF (10 ng/ml) induced an increase (two-fold) in glucose transport. This effect was transient and independent of protein synthesis.
Murphy
S
S
Department of Pharmacology, College of Medicine, University of Iowa, Iowa City 52242.
Welk
G
G
Kardos
S
S
D001254:Astrocytoma / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D003847:Deoxyglucose / Q000493:pharmacokinetics; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005947:Glucose / Q000493:pharmacokinetics*; D006801:Humans; D013997:Time Factors; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D004815:Epidermal Growth Factor; D003513:Cycloheximide; D003847:Deoxyglucose; D005947:Glucose