Assay for opsonin activity based on chemiluminescence of a Cypiridina luciferin analog.
Opsonin activity (OA) was investigated in serum samples from cord blood of 11 full-term newborn infants and 12 healthy adult volunteers by the method of luminol-dependent chemiluminescence (L-CL) and Cypiridina luciferin analog-dependent chemiluminescence (CLA-CL) together with the measurement of complement components (C3, C4, C3A and properdin). CL and complement components of cord samples were significantly lower than that of healthy adult blood. CLA-CL showed a significant correlationship to C3 and C3A. L-CL also displayed a significant correlationship to C3A but the relationship to C3 was not significant. From these results, when OA is measured by CL, CLA-CL is preferred to L-CL as a technique.
Nishida
A
A
Neonatal Unit, Children's Hospital Hachiouji, Tokyo, Japan.
Kimura
H
H
Nakano
M
M
D000328:Adult; D003176:Complement C3 / Q000378:metabolism; D015926:Complement C3a / Q000378:metabolism; D003181:Complement C4 / Q000378:metabolism; D005312:Fetal Blood / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D009895:Opsonin Proteins / Q000097:blood*; D011414:Properdin / Q000378:metabolism; D011719:Pyrazines / Q000494:pharmacology*
D003176:Complement C3; D003181:Complement C4; D009895:Opsonin Proteins; D011719:Pyrazines; C048193:2-methyl-6-phenyl-3,7-dihydroimidazo(1,2-a)pyrazin-3-one; D011414:Properdin; D008165:Luminol; D015926:Complement C3a