High performance liquid chromatography of spermidine and spermine using a postcolumn reactor of immobilized polyamine oxidase (Aspergillus terreus) followed by electrochemical detection.
A highly sensitive and selective method for the determination of spermidine and spermine has been developed. A polyamine oxidase (Aspergillus terreus) immobilized column was used as a postcolumn reactor. The detection limit was 0.2 pmol/injection for both spermidine and spermine with a linear range of three orders of magnitude.
Watanabe
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Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
D001230:Aspergillus / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D006801:Humans; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D013095:Spermidine / Q000652:urine*; D013096:Spermine / Q000652:urine*; D000097667:Polyamine Oxidase
D004800:Enzymes, Immobilized; D000587:Oxidoreductases Acting on CH-NH Group Donors; D013095:Spermidine; D013096:Spermine; D000097667:Polyamine Oxidase