Phenotypic and functional analysis of tumour-infiltrating lymphocytes from patients with melanoma and other metastatic cancers.
Thirty tumour specimens, among which were 17 melanomas, were cultured with recombinant interleukin-2 (IL-2) in order to produce tumour-infiltrating lymphocytes (TIL). In the melanomas, three categories of TIL were characterised. The first, containing mostly CD3+ and CD8+ cells, lysed only autologous tumour cells; the second, containing mostly CD3+ and CD4+ cells, lysed both autologous tumour cells and allogeneic cells lines; the third, with mixed phenotype although cytotoxic for K562 targets, did not kill melanoma cells. The optimal conditions for a good development of TIL were established: we found that the lymph node or cutaneous origin of the tumour was unimportant, a 2 h enzymatic treatment was optimum and that TIL grew well in AIM V serum free medium. Therefore the easiness and the reproducibility of the TIL cultures from melanoma tumour samples allows the rapid development of therapeutic trials in metastatic melanoma.
Mathiot
C
C
Laboratoire d'Immunologie Clinique, Institut curie, Paris, France.
Robin
E
E
Gey
A
A
Weng
X
X
Dorval
T
T
Pouillart
P
P
Sastre
X
X
Zerbib
M
M
Hamelin
J P
JP
Salmon
R
R
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D007700:Kinetics; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008297:Male; D008545:Melanoma / Q000276:immunology* / Q000556:secondary*; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology; D012878:Skin Neoplasms / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
D015703:Antigens, CD; D007376:Interleukin-2