Interleukin-3 induces translocation and down-regulation of protein kinase C in human platelets.
Protein kinase C (PKC) is a family of phospholipid-dependent kinases that is involved, along with calcium mobilization, in the activation of human platelets. Since interleukin-3 (IL-3) has been shown to act, in part, by activating PKC, we investigated the effect of IL-3 on PKC activity and content in human platelets. Exposure of platelets to 10 ng/ml of IL-3 was associated with a rapid (i.e., within 3 minutes) translocation of PKC activity and content from the cytosol to the membrane fraction. In addition, treatment with IL-3 effected a time-dependent down-regulation of PKC activity and content. We speculate that IL-3 may act as a modulator of PKC-dependent pathways in the human platelet.
Cook
P P
PP
Department of Medicine, East Carolina University School of Medicine, Greenville, North Carolina 27858-4354.
Chen
J
J
Ways
D K
DK
D001792:Blood Platelets / Q000201:enzymology*; D002451:Cell Compartmentation / Q000187:drug effects; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D015536:Down-Regulation / Q000187:drug effects; D006657:Histones / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000494:pharmacology*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*
D006657:Histones; D007377:Interleukin-3; D011493:Protein Kinase C