Activation of Lyt-2 associated with distant upstream insertion of an SL3-3 provirus.
Two Lyt-2+ mutants of the T-cell lymphoma SL12.4.10 were selected by fluorescence activated cell sorting. Both mutants expressed Lyt-2 (CD8 alpha-chain) but not Lyt-3 (CD8 beta-chain). Derivatives of one Lyt-2+ mutant that expressed Lyt-3 could be isolated by sorting for Lyt-3+ cells. Southern blotting analysis indicated that both mutants had structural rearrangements within or immediately 3' of the Lyt-3 gene, accompanied by demethylation of at least one Hpa II site within the Lyt-2 gene. Gene cloning analysis of one mutant demonstrated that the structural rearrangement was due to insertion of an SL3-3 provirus 35 kb 5' to the Lyt-2 gene. It is likely that Lyt-2 gene activation is a direct or indirect consequence of proviral insertion at this site.
Anson
D S
DS
Department of Cancer Biology, Salk Institute, San Diego, CA 92186-5800.
Clarkin
K
K
Hyman
R
R
D000818:Animals; D000950:Antigens, Ly / Q000235:genetics*; D001483:Base Sequence; D016827:CD8 Antigens / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D015321:Gene Rearrangement; D016399:Lymphoma, T-Cell / Q000235:genetics; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011533:Proviruses / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics*; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D000950:Antigens, Ly; D016827:CD8 Antigens; D004279:DNA, Viral