Clinical and immunological effects of single bolus administration of recombinant interleukin-2 in cattle.
Recombinant bovine interleukin-2 (rBoIL-2) was administered as a single intramuscular bolus to healthy calves to determine the minimal dose capable of exerting a biological response. Doses ranging from 2.5 to 0.05 micrograms rBoIL-2/kg did not induce pyrexia, diarrhea, or depression, nor did they alter any blood chemistry or hematological parameters commonly associated with IL-2 toxicity. Moreover, the only significant immunological change observed was a reduction in the number of peripheral blood lymphocytes identified with the monoclonal antibodies B7A, BAQ4A (WC1+ cells), CACTB6A (WC2+ cells) and DH59B (monocytes). The decrease in cells associated with these markers did not influence non-MHC restricted cytotoxicity or in vitro lymphocyte proliferative responses to mitogens and IL-2. The treatments had no effect on delayed type hypersensitivity responses to phytohemagglutinin. These results indicate that IL-2 may be involved in the regulation of trafficking patterns of a unique subpopulation of lymphocytes in cattle.
Campos
M
M
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon.
Hughes
H P
HP
Godson
D L
DL
Sordillo
L M
LM
Rossi-Campos
A
A
Babiuk
L A
LA
D000818:Animals; D002417:Cattle / Q000097:blood / Q000276:immunology* / Q000502:physiology; D006968:Hypersensitivity, Delayed / Q000662:veterinary; D007111:Immunity, Cellular / Q000187:drug effects; D007273:Injections, Intramuscular / Q000662:veterinary; D007376:Interleukin-2 / Q000008:administration & dosage / Q000633:toxicity*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D011897:Random Allocation; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000633:toxicity
D007376:Interleukin-2; D011994:Recombinant Proteins