Penetration of Eimeria tenella sporozoites under different oxygen concentrations in vitro.
Sporozoites of Eimeria tenella (Wisconsin strain) were inoculated onto monolayers of normal chicken kidney fibroblasts and cultured in RPMI-1640 supplemented with 5% fetal bovine serum, sodium bicarbonate, and gentamicin under either aerobic, 5% CO2/95% air, or anaerobic conditions. Penetration of fibroblasts by sporozoites under CO2 or anaerobic conditions at 2 and 24 hr postinoculation was 3-4 times greater than that in the aerobic atmosphere. Effect of reduced oxygen concentrations, i.e., 20.0, 12.5, and 5.0% oxygen, was also investigated in an N2-O2-CO2 incubator. Under 5.0 and 12.5% oxygen at 2 and 24 hr postinoculation, the number of sporozoites that penetrated was about 4 and 2 times greater, respectively, than under 20.0% O2. These results indicate that lower oxygen concentrations provide for greater penetration by E. tenella sporozoites in cultured cells.
Fukata
T
T
College of Agriculture, University of Osaka Prefecture, Japan.
Sasai
K
K
Arakawa
A
A
McDougald
L R
LR
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002478:Cells, Cultured; D016786:Eimeria tenella / Q000502:physiology*; D005347:Fibroblasts / Q000469:parasitology*; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*
D002245:Carbon Dioxide; D009584:Nitrogen; D010100:Oxygen