Effect of dietary curcumin and ascorbyl palmitate on azoxymethanol-induced colonic epithelial cell proliferation and focal areas of dysplasia.
Curcumin, a major yellow pigment of turmeric obtained from powdered rhizomes of the plant Curcuma longa Linn., is commonly used as a coloring agent in foods, drugs and cosmetics. Ascorbyl palmitate is a lipid soluble derivative of ascorbic acid. Both curcumin and ascorbyl palmitate have antioxidant activity and are potent inhibitors of 12-O-tetradecanoyl-phorbol-13-acetate-induced tumor promotion in mouse skin. The effects of dietary curcumin and ascorbyl palmitate on azoxymethanol (AOM)-induced hyperproliferation of colonic epithelial cells and the incidence of focal areas of dysplasia (FADs) were evaluated in female CF-1 mice fed an AIN 76A diet. Subcutaneous injections of AOM (10 mg/kg body wt. once weekly for 6 weeks) caused hyperplasia and the formation of FADs in the colon. Administration of 2% curcumin in the diet inhibited AOM-induced formation of FADs while administration of 2% ascorbyl palmitate in the diet did not demonstrate inhibition. This result suggests that dietary curcumin may inhibit AOM-induced colonic neoplasia in mice.
Huang
M T
MT
Department of Chemical Biology and Pharmacognosy, College of Pharmacy, Rutgers University, Piscataway, NJ 08855-0789.
Deschner
E E
EE
Newmark
H L
HL
Wang
Z Y
ZY
Ferraro
T A
TA
Conney
A H
AH
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D001835:Body Weight; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology*; D004032:Diet; D004848:Epithelium / Q000187:drug effects; D005260:Female; D008746:Methylazoxymethanol Acetate / Q000031:analogs & derivatives* / Q000633:toxicity; D051379:Mice; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D000975:Antioxidants; D008746:Methylazoxymethanol Acetate; D003474:Curcumin; C030667:methylazoxymethanol; D013755:Tetradecanoylphorbol Acetate; D001205:Ascorbic Acid; C031226:6-O-palmitoylascorbic acid