title: Cloning and nucleotide sequence of the gene encoding the gamma-D-glutamyl-L-diamino acid endopeptidase II of Bacillus sphaericus.
abstract: The gene encoding the Bacillus sphaericus gamma-D-glutamyl-L-diamino acid endopeptidase II, a cytoplasmic enzyme involved in cell sporulation [1], contains the information for a 271-amino acid protein devoid of a signal peptide. The endopeptidase lacks sequence relatedness with other proteins of known primary structure except that its C-terminal region has significant similarity with the C-terminal region of the 54-kDa P54 protein of Enterococcus faecium, of unknown function [2].
authors.affiliation: Centre d'Ingénierie des Protéines, Université de Liège, Sart Tilman, Belgium.
authors.forename: M L
authors.initials: ML
authors.lastname: Hourdou
authors.forename: C
authors.initials: C
authors.lastname: Duez
authors.forename: B
authors.initials: B
authors.lastname: Joris
authors.forename: M J
authors.initials: MJ
authors.lastname: Vacheron
authors.forename: M
authors.initials: M
authors.lastname: Guinand
authors.forename: G
authors.initials: G
authors.lastname: Michel
authors.forename: J M
authors.initials: JM
authors.lastname: Ghuysen
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D010450:Endopeptidases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D004269:DNA, Bacterial; D010450:Endopeptidases; C073797:gamma-D-glutamyl-L-di-amino acid endopeptidase II