Effect of methotrexate on the germination and growth of Aspergillus fumigatus and Aspergillus flavus strains.
Methotrexate at concentrations of greater than or equal to 10(-5) M caused a significant reduction in the rate of germ tube formation of spores of Aspergillus fumigatus, but not of Aspergillus flavus, after 7 h incubation. At 10(-3) M the drug caused a significant reduction in germ tube elongation of A. fumigatus and A. flavus strains during the first 7 h of germination. After 18 h incubation, drug concentrations of greater than or equal to 4 x 10(-4) M produced greater than 80% reduction in growth of all strains tested. Potentiation of the antifungal effect against A. fumigatus was detected in tests in which methotrexate at 10(-4) M was used in combination with amphotericin B at 5 x 10(-7) M.
Warnock
D W
DW
Department of Microbiology, Bristol Royal Infirmary, UK.
Oliver
D A
DA
Cheung
M M
MM
Zurick
N J
NJ
D000666:Amphotericin B / Q000494:pharmacology*; D001231:Aspergillus flavus / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D004347:Drug Interactions; D004359:Drug Therapy, Combination / Q000494:pharmacology; D008727:Methotrexate / Q000494:pharmacology*; D013045:Species Specificity; D013172:Spores, Fungal / Q000187:drug effects*
D000666:Amphotericin B; D008727:Methotrexate