Functional classification of simian immunodeficiency virus isolated from a chimpanzee by transactivators.
In reporter-based transient expression systems, we characterized simian immunodeficiency virus from a chimpanzee (SIVCPZ), with special reference to the human immunodeficiency virus type 1 (HIV-1). SIVCPZ was not equally activated by tat and rev transactivators derived from representative primate lentiviruses. HIV-1 alone activated SIVCPZ to the full extent in both tat and rev assays. The tat and rev gene products of SIVCPZ, as well as those of HIV-1, efficiently transactivated the other viruses. These results indicate that SIVCPZ is identical to HIV-1 with regard to the compatibility of tat and rev gene activities among four subgroups of primate lentiviruses.
Sakuragi
J
J
Department of Virai Oncology, Kyoto University, Japan.
Sakai
H
H
Sakuragi
S
S
Shibata
R
R
Wain-Hobson
S
S
Hayami
M
M
Adachi
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D004274:DNA, Recombinant / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D015694:Gene Products, rev / Q000096:biosynthesis / Q000235:genetics; D015696:Gene Products, tat / Q000096:biosynthesis / Q000235:genetics; D015497:HIV-1 / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D002679:Pan troglodytes; D012367:RNA, Viral / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D015302:Simian Immunodeficiency Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics*; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
D004274:DNA, Recombinant; D015694:Gene Products, rev; D015696:Gene Products, tat; D012367:RNA, Viral; D015534:Trans-Activators; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus; D015500:Chloramphenicol O-Acetyltransferase