Aerobic heat shock activates trehalose synthesis in embryos of Artemia franciscana.
Encysted embryos (cysts) of the brine shrimp, Artemia franciscana, contain large amounts of trehalose which they use as a major substrate for energy metabolism and biosynthesis for development under aerobic conditions at 25 degrees C. When cysts are placed at 42 degrees C (heat shock) these pathways stop, and the cysts re-synthesize the trehalose that was utilized during the previous incubation at 25 degrees C. Glycogen and glycerol, produced from trehalose at 25 degrees C, appear to be substrates for trehalose synthesis during heat shock. Anoxia prevents trehalose synthesis in cysts undergoing heat shock. These results are consistent with the view that trehalose may play a protective role in cells exposed to heat shock, and other environmental insults, in addition to being a storage form of energy and organic carbon for development.
Clegg
J S
JS
University of California, Davis Bodega Marine Laboratory, Bodega Bay 94923.
Jackson
S A
SA
D000818:Animals; D001156:Artemia / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D004734:Energy Metabolism; D005990:Glycerol / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006358:Hot Temperature; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D013379:Substrate Specificity; D014199:Trehalose / Q000096:biosynthesis* / Q000378:metabolism
D006003:Glycogen; D014199:Trehalose; D005990:Glycerol; D010100:Oxygen