Determination of hydroxychloroquine and its major metabolites in plasma using sequential achiral-chiral high-performance liquid chromatography.
A sequential achiral-chiral high-performance liquid chromatographic system has been developed for the determination of the enantiomers of hydroxychloroquine, (+)-HCQ and (-)-HCQ, and the enantiomers of its three major metabolites, bisdesethylchloroquine, desethylhydroxychloroquine and desethylchloroquine, in plasma. The HCQ was separated from the metabolites and interfering components in the plasma and quantified on a cyano-bonded phase, and the enantiomeric composition determined using a Chiral-AGP chiral stationary phase. The assay was validated and applied to the analysis of a pilot study of the pharmacokinetics of (+)- and (-)-HCQ in rabbits.
Iredale
J
J
Department of Oncology, McGill University, Montreal, Quebec, Canada.
Wainer
I W
IW
D000818:Animals; D001682:Biological Availability; D002738:Chloroquine / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D006886:Hydroxychloroquine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D006886:Hydroxychloroquine; C049283:cletoquine; D002738:Chloroquine; C033490:N,N-dideethylchloroquine; C007278:desethylchloroquine