Lens opacification and H2O2 elevation induced by a tumor promoter.
Incubation of whole bovine lens with 10(-7) M 12-O-tetradecanoylphorbol-13-acetate (TPA) led to the lens opacity within 24 h. The hydrogen peroxide (H2O2) concentration in the whole lens was elevated 4 fold after treatment with either 10(-7) M TPA or 2.5 mM glucose/20 microM glucose oxidase. The lens opacification and H2O2 elevation were TPA dose-dependent. Preincubation of the lens with anti-tumor promoting agents EGCG (epigallocatechin gallate) or Sarp A (sarcophytol A) stopped the TPA-mediated opacification process and suppressed H2O2 elevation.
Ye
J J
JJ
Department of Physiology, New York University Medical Center, NY 10016.
Frenkel
K
K
Zadunaisky
J A
JA
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D002392:Catechin / Q000031:analogs & derivatives / Q000627:therapeutic use; D002417:Cattle; D004224:Diterpenes / Q000627:therapeutic use; D005947:Glucose; D005949:Glucose Oxidase; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007908:Lens, Crystalline / Q000737:chemistry* / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*
D000970:Antineoplastic Agents; D004224:Diterpenes; C058761:sarcophytol A; D002392:Catechin; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate; D005949:Glucose Oxidase; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate