title: Lipid peroxidation during myocardial reperfusion.
abstract: Reperfusion of heart muscle after prolonged ischaemia is associated with metabolic and functional abnormalities and eventual cell death. Free radical induced lipid peroxidation of cell membranes is thought to be a major mechanism in the evolution of reperfusion damage. The evidences in support for this kind of damage are based on tissue malondialdehyde quantitation by the thiobarbituric acid test (TBA-test). In an attempt to verify this topic we have subjected isolated and Langendorff perfused rabbit hearts to a period of 60 minutes of severe ischaemia plus 30 minutes of reperfusion. At appropriate time points malondialdehyde was determined in the tissue by means of TBA-test and directly by reversed phase, high pressure, liquid chromatography (HPLC). We have found no correlation between the two compared assays. During reperfusion, there was the formation of non-lipid related, malondialdehyde-like, TBA-reactive substance which leads to overestimations of the extent of lipid peroxidation. On the contrary, by direct HPLC quantitation, there was a decrease of tissue malondialdehyde during ischaemia and during the early phases of reperfusion. Our results demonstrate that TBA-test is not a reliable index of malondialdehyde accumulation in organ system.
authors.affiliation: Cattedra di Cardiologia, Università degli Studi di Brescia, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Ceconi
authors.forename: A
authors.initials: A
authors.lastname: Cargnoni
authors.forename: E
authors.initials: E
authors.lastname: Pasini
authors.forename: E
authors.initials: E
authors.lastname: Condorelli
authors.forename: S
authors.initials: S
authors.lastname: Currello
authors.forename: R
authors.initials: R
authors.lastname: Ferrari
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003402:Creatine Kinase / Q000378:metabolism*; D066298:In Vitro Techniques; D007511:Ischemia / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D011817:Rabbits
chemical_list: D008315:Malondialdehyde; D003402:Creatine Kinase