title: Effects of laparotomy on systemic macrophage function.
abstract: Surgical trauma induces immunosuppression that may adversely influence survival. This study examined the effect of laparotomy on two different macrophage populations, peritoneal macrophages (PM phi) and Kupffer cells. Female, 6- to 8-week old, CFW/C3H-HeN mice (n = 160) were randomly allocated to one of three study groups: control, ether anesthetic only, or ether anesthetic and laparotomy. On postoperative days 1 and 3, PM phis and Kupffer cells were harvested and assayed for superoxide anion production (O2-), percent macrophage phagocytosis of Candida albicans (CAP), percent C. albicans killed by macrophages (CAK), percent major histocompatibility complex (MHC)-class II antigen expression, and antigen presentation. Macrophages isolated on postoperative day 1 were also cocultured with 100 units/10(6) cells/ml interferon-gamma (IFN-gamma). Laparotomy significantly impaired microbicidal activity (O2-, percent CAP, and percent CAK) and antigen presentation on postoperative day 1. On postoperative day 3, O2- and antigen presentation were increased significantly (p less than 0.05) over control values, indicating a rebound phenomenon. Kupffer cell microbicidal function was unchanged on postoperative days 1 and 3. The initial immune impairment (PM phis: O2-, CAP, and CAK) was abrogated by IFN-gamma treatment. In immunosuppressed hosts after injury, administration of macrophage-activating factors such as IFN-gamma could be of therapeutic benefit.
authors.affiliation: Harrison Department of Surgical Research, University of Pennsylvania School of Medicine, Philadelphia.
authors.forename: H P
authors.initials: HP
authors.lastname: Redmond
authors.forename: K
authors.initials: K
authors.lastname: Hofmann
authors.forename: J
authors.initials: J
authors.lastname: Shou
authors.forename: P
authors.initials: P
authors.lastname: Leon
authors.forename: C J
authors.initials: CJ
authors.lastname: Kelly
authors.forename: J M
authors.initials: JM
authors.lastname: Daly
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D002176:Candida albicans; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D005856:Germ-Free Life; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007813:Laparotomy; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D010100:Oxygen / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis; D011184:Postoperative Period
chemical_list: D000949:Histocompatibility Antigens Class II; D015232:Dinoprostone; D010100:Oxygen