An examination of the effects of solutions containing histidine and lactobionate for heart, pancreas, and liver preservation in the rat.
Fifty-five rat pancreas transplants, 18 rat heart transplants, and 41 rat liver transplants were performed using standard UW solution, the new HL solution (HL-I), or a modified HL solution (HL-II). Storage times of 18 hr were used in the heart preservation experiments, 24 hr in the liver preservation experiments, and 48 or 72 hr in the pancreas preservation experiments. HL-I solution was superior to both HL-II and UW solution for heart preservation (1-week graft survival rates of 100% [7/7], 0% [0/5], and 50% [3/6], respectively). HL-I and HL-II were superior to UW for 24 hr liver preservation (1-week graft survival rates of 78% [11/14], 80% [8/10], and 29% [5/17], respectively). In contrast, HL-II was superior to both HL-I and UW solutions for pancreas preservation following both 48-hr preservation and 72-hr preservation. Satisfactory graft function was achieved in 100% (7/7), 40% (6/15), and 44.4% (4/9) of pancreases transplanted after 48 hr using HL-II, HL-I, and UW solutions, respectively, and in 50% (4/8), 0% (0/8), and 0% (0/8) following 72-hr preservation. Histidine- and lactobionate-containing solutions thus represent a further improvement in organ preservation by simple cold storage.
Sumimoto
R
R
Second Department of Surgery, Hiroshima University, School of Medicine, Japan.
Dohi
K
K
Urushihara
T
T
Jamieson
N V
NV
Ito
H
H
Sumimoto
K
K
Fukuda
Y
Y
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004187:Disaccharides / Q000494:pharmacology*; D005978:Glutathione; D006321:Heart; D016027:Heart Transplantation / Q000502:physiology; D006639:Histidine / Q000494:pharmacology*; D007328:Insulin; D008099:Liver; D016031:Liver Transplantation / Q000502:physiology; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010179:Pancreas; D016035:Pancreas Transplantation / Q000502:physiology; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D012996:Solutions / Q000494:pharmacology*
D004187:Disaccharides; C075029:HL-I solution; C075030:HL-II solution; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D006639:Histidine; D000493:Allopurinol; C005608:lactobionic acid; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose