Haemocompatibility of polymer having phospholipid polar groups evaluated by monoclonal antibody method.
The haemocompatibility of a polymer having the phospholipid polar group, poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate(BMA)), was evaluated by a monoclonal antibody SZ-21 (directing specifically against the membrane glycoprotein complex IIIa of human platelet) radioimmunoassay method. The study showed that poly(MPC-co-BMA) possessed better haemocompatibility than segmented polyurethane (SPEU). Radioactivity of platelets adhered (counts per min/cm) on a polyethylene (PE) tube coated with poly(MPC-co-BMA) was 19.51 +/- 5.58, with albumin; 20.05 +/- 7.29, with SPEU having sulphonated group; 76.10 +/- 18.9, with SPEU; 287.0 +/- 95.0, PE treated by plasma irradiation; 2091 +/- 261, and original PE was 1772 +/- 116. The results were further certified by scanning electron microscopic observations.
Xi
T
T
Center of Medical Devices, National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China.
Tian
W
W
Lei
X
X
Zhao
L
L
Ishihara
K
K
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D010743:Phospholipids / Q000737:chemistry; D010973:Platelet Adhesiveness; D011109:Polymethacrylic Acids / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D013927:Thrombosis / Q000517:prevention & control
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D010743:Phospholipids; D011109:Polymethacrylic Acids; D011140:Polyurethanes