Rational attenuation of Corynebacterium pseudotuberculosis: potential cheesy gland vaccine and live delivery vehicle.
The phospholipase D (PLD) gene (pld) has been deleted from the Corynebacterium pseudotuberculosis chromosome by using site-specific mutagenesis. Sheep infection trials indicate that the PLD-negative C. pseudotuberculosis strain (Toxminus) is incapable of inducing caseous lymphadentis (cheesy gland) even at doses two logs higher than that at which the wild-type strain produces the disease. This clearly establishes PLD as a major C. pseudotuberculosis virulence factor. Vaccination of sheep with live Toxminus C. pseudotuberculosis elicits strong humoral and cell-mediated immune responses and protects the animals from wild-type challenge.
Hodgson
A L
AL
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.
Krywult
J
J
Corner
L A
LA
Rothel
J S
JS
Radford
A J
AJ
D000818:Animals; D000917:Antibody Formation; D002874:Chromosome Mapping; D003354:Corynebacterium Infections / Q000276:immunology* / Q000517:prevention & control*; D016925:Corynebacterium pseudotuberculosis / Q000472:pathogenicity*; D004306:Dose-Response Relationship, Immunologic; D007116:Immunization, Passive; D007371:Interferon-gamma / Q000096:biosynthesis; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D010739:Phospholipase D / Q000235:genetics / Q000276:immunology; D010957:Plasmids; D012756:Sheep; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000096:biosynthesis*; D014774:Virulence / Q000235:genetics
D014613:Vaccines, Attenuated; D007371:Interferon-gamma; D010739:Phospholipase D