Characterization of bone forming cells in posttraumatic myositis ossificans by lectins.
Lectins were used to characterize bone forming cells in posttraumatic myositis ossificans. The lectins applied were as follows: Arachis hypogaea (PNA): specific for beta-D-galactose (1,3)N-acetyl-D-galactosamine (Gal-1,3 GalNac), Canavalia ensiformis (Con A): specific for alpha-D-glucose (D-Glc) and alpha-D-mannose (D-Man) and Wheat germ (WGA): specific for N-acetyl(1,4)D-glucosamine (Glc-NaC) and neuraminic acid. The development of myositis ossificans was characterized by the appearance of a WGA binding cell population. The lectin-binding sites appeared as a cluster in the supranuclear cytoplasm, corresponding to the Golgi-complex. However, the WGA lectin-binding sites disappeared in the mature form of myositis ossificans. We assume that these lectin binding cells may be the bone marrow derived precursors of myofibroblast-like cells which are responsible for bone formation within the damaged muscle.
Illés
T
T
Department of Orthopaedics, University Medical School of Pécs, Hungary.
Dubousset
J
J
Szendröi
M
M
Fischer
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D001846:Bone Development / Q000502:physiology*; D001842:Bone and Bones / Q000293:injuries*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D037102:Lectins; D008297:Male; D008875:Middle Aged; D009221:Myositis Ossificans / Q000473:pathology*
D006023:Glycoproteins; D037102:Lectins