SwaI, a unique restriction endonuclease from Staphylococcus warneri, which recognizes 5'-ATTTAAAT-3'.
A novel class-II restriction endonuclease designated SwaI was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, pBR322 or pBR328 DNA. SwaI recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. SwaI was used to digest chromosomal DNA from various microorganisms and human cells.
Lechner
M
M
Boehringer Mannheim GmbH, Biochemical Research Center, Penzberg, Germany.
Frey
B
B
Laue
F
F
Anton-Botella
J
J
Smith
C L
CL
Ankenbauer
W
W
Schmitz
G G
GG
D004247:DNA / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D013210:Staphylococcus / Q000201:enzymology*; D013379:Substrate Specificity
D004247:DNA; C074767:endodeoxyribonuclease SwaI; D015252:Deoxyribonucleases, Type II Site-Specific