Cloning and characterization of a repetitive sequence from Pneumocystis carinii.
Four repetitive sequence clones measuring 10.9-23.4 kb in length were isolated from the genomic library of Pneumocystis carinii. Restriction enzymes mapping and cross-hybridization studies revealed that these clones are interrelated and that they derive from the common repeat unit, which is specific for P. carinii. Dot-blot analysis suggested that the copy number of the repeat sequence is about 100, assuming that the genome size is 1.5 x 10(7) bp. Interestingly, the repetition unit extended over at least 23.4 kb and included long, 5.2-kb inverted repeats, for example, A-B-A'-C, in which A' is the inversion of A.
Watanabe
J
J
Department of Parasitology, University of Tokyo, Japan.
Nakata
K
K
Nashimoto
H
H
Ikeda
H
H
D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D011010:Pneumocystis / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping
D015342:DNA Probes; D004271:DNA, Fungal