title: Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression.
abstract: Jasmonic acid (JA) and its methyl ester, methyl jasmonate (MeJA), are plant lipid derivatives that resemble mammalian eicosanoids in structure and biosynthesis. These compounds are proposed to play a role in plant wound and pathogen responses. Here we report the quantitative determination of JA/MeJA in planta by a procedure based on the use of [13C,2H3]MeJA as an internal standard. Wounded soybean (Glycine max [L] Merr. cv. Williams) stems rapidly accumulated MeJA and JA. Addition of MeJA to soybean suspension cultures also increased mRNA levels for three wound-responsive genes (chalcone synthase, vegetative storage protein, and proline-rich cell wall protein) suggesting a role for MeJA/JA in the mediation of several changes in gene expression associated with the plants' response to wounding.
authors.affiliation: Biotechnology Center, Ohio State University, Columbus 43210.
authors.forename: R A
authors.initials: RA
authors.lastname: Creelman
authors.forename: M L
authors.initials: ML
authors.lastname: Tierney
authors.forename: J E
authors.initials: JE
authors.lastname: Mullet
mesh_terms: D000217:Acyltransferases / Q000235:genetics; D002473:Cell Wall; D003517:Cyclopentanes / Q000737:chemistry; D005786:Gene Expression Regulation; D013058:Mass Spectrometry; D054883:Oxylipins; D014674:Plant Proteins, Dietary / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013025:Glycine max / Q000235:genetics / Q000502:physiology*; D014945:Wound Healing
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D014674:Plant Proteins, Dietary; D012333:RNA, Messenger; C011006:jasmonic acid; D000217:Acyltransferases; C020141:flavanone synthetase