A new modification for preparing tissue blocks for cryostat sectioning.
A modification of the Carter method for preparing tissue blocks for cryostat sectioning is presented. One problem with the Carter method is that tissue edges occasionally freeze above the plane of the smooth cryostat disk. My modification avoids this problem by gradual liquid nitrogen cooling of the disk. This enables all tissue edges to be applied to the smooth disk surface until freeze adherence occurs.
Koehn
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D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D005629:Frozen Sections / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D012867:Skin / Q000473:pathology*