title: The isolation and partial characterization of trypsinogen, pancreatic secretory trypsin inhibitor and multiple forms of chymotrypsinogen and trypsin from the pancreas of the ostrich (Struthio camelus).
abstract: 1. PSTI, two chymotrypsinogens and two trypsins were purified to homogeneity by acid extraction, salt fractionation, SP-Sephadex C-50 chromatography and RP-HPLC. 2. A third chymotrypsinogen, a trypsinogen and another trypsin were purified using an alkaline extraction procedure, followed by Trasylol- and Benzamidine-Sepharose affinity chromatography and hydroxylapatite chromatography. 3. The enzymes differed in amino acid composition as well as in specific activities towards synthetic amidase and esterase substrates. 4. N-terminal amino acid sequences were determined for one chymotrypsinogen and one trypsin.
authors.affiliation: Department of Biochemistry, University of Port Elizabeth, South Africa.
authors.forename: N
authors.initials: N
authors.lastname: Smith
authors.forename: R J
authors.initials: RJ
authors.lastname: Naudé
authors.forename: W
authors.initials: W
authors.lastname: Oelofsen
authors.forename: C
authors.initials: C
authors.lastname: Lazure
authors.forename: A
authors.initials: A
authors.lastname: Patthy
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001717:Birds; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002919:Chymotrypsinogen / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid; D014357:Trypsin / Q000302:isolation & purification* / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D014362:Trypsinogen / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D014361:Trypsin Inhibitors; D014362:Trypsinogen; D002919:Chymotrypsinogen; D014357:Trypsin