Transfection of chronic lymphocytic leukemic cells with myc and ras.
Thirty samples of cells from twenty-one cases of chronic lymphocytic leukemia (CLL) were transfected with myc and/or ras. Transformation, as assessed by growth in liquid culture and change in morphology, occurred in only one case. Colony culture in semi-solid media, however, does not occur with these cells. It would appear that the neoplastic genotype of CLL cells does not predispose the cells to readily transform, even with transfection with these two oncogenes.
Zheng
C Y
CY
Department of Pathology and Cancer Centre, University of Saskatachewan, Sasktoon, Canada.
Skinnider
L F
LF
Xian
J
J
Maksymiuk
A
A
D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000473:pathology; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D010641:Phenotype; D014162:Transfection / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000473:pathology