title: A collaborative study to examine the sensitivity and reproducibility of assays for the detection of DNA in biologicals derived from continuous cell lines.
abstract: This report describes the results of a collaborative study which was set up to examine the sensitivity and reproducibility of DNA assays performed by different laboratories. Fifteen laboratories analysed 12 samples containing different amounts of DNA and protein. The study revealed a high degree of variability between laboratories in determining the DNA content of the test samples.
authors.affiliation: National Institute for Biological Standards and Control, South Mimms, Herts, U.K.
authors.forename: J S
authors.initials: JS
authors.lastname: Robertson
authors.forename: A B
authors.initials: AB
authors.lastname: Heath
mesh_terms: D000818:Animals; D001688:Biological Products / Q000032:analysis* / Q000302:isolation & purification / Q000592:standards; D002460:Cell Line; D004247:DNA / Q000032:analysis* / Q000592:standards; D015342:DNA Probes; D004340:Drug Contamination; D005069:Evaluation Studies as Topic; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000302:isolation & purification; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001688:Biological Products; D015342:DNA Probes; D011506:Proteins; D004247:DNA