Cytogenetic analysis in ataxia telangiectasia with malignant lymphoma.
We present the results of cytogenetic analysis in a brother and sister with ataxia telangiectasia (AT), one of whom had malignant T-cell lymphoma. In both children, cytogenetic analysis of phytohemagglutinin (PHA)-stimulated lymphocytes showed chromosomal instability and inv(7) in 10% of the cells examined. The malignant lymphoma was analyzed cytogenetically on slides obtained from short-term culture of the lymph node cells; 64 cells were analyzed. A heterogeneous cell population was noted. Fourteen cells (21.9%) had a normal male karyotype; t(7;14)(p14;q12) and inv(7)(p14q35) were observed in 6.3% and 3.1% of metaphases. Owing to low frequency, these cells are probably a characteristic of the basic disease and have no features of malignant cells. Forty cells (62.5%) had a pseudodiploid karyotype 46,XY,dup(1)(p22p36),del(5)(q33),del(12)(p11), without cytogenetically evident aberrations of chromosomes 7 and 14. The results of these investigations suggest that the cells with rearrangements of chromosomes 1, 5, and 12 are malignant cells and did not originate by transformation of cells with inv(7) and t(7;14).
Petković
I
I
Institute for Mother and Child Health, Clinical Hospital Dr. O. Novosel, Zagreb, Yugoslavia.
Ligutić
I
I
Dominis
M
M
Loffler-Badzak
D
D
Cepulić
M
M
Nakić
M
M
D001260:Ataxia Telangiectasia / Q000150:complications / Q000235:genetics* / Q000473:pathology; D002648:Child; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002881:Chromosomes, Human, Pair 12; D002895:Chromosomes, Human, Pair 5; D005260:Female; D006801:Humans; D016399:Lymphoma, T-Cell / Q000150:complications / Q000235:genetics* / Q000473:pathology; D008297:Male; D014407:Tumor Cells, Cultured