title: The nucleotide sequence of an infectious insect-transmissible clone of the geminivirus Panicum streak virus.
abstract: The infectious genome of a Kenyan isolate of Panicum streak virus (PSV) has been cloned and sequenced. Infection of host plants was done using an Agrobacterium binary vector containing a partial repeat of the genome. Progeny virus from resultant infections proved to be transmissible by the leafhopper Cicadulina mbila (Naude). Comparisons of the amino acid sequences of PSV DNA-encoded proteins with those of previously characterized geminiviruses infecting monocotyledonous plants, including maize streak virus, revealed high levels of identity. The evolutionary relationship between PSV and other geminiviruses infecting monocotyledons is discussed.
authors.affiliation: Department of Virus Research, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, U.K.
authors.forename: R W
authors.initials: RW
authors.lastname: Briddon
authors.forename: P
authors.initials: P
authors.lastname: Lunness
authors.forename: L C
authors.initials: LC
authors.lastname: Chamberlin
authors.forename: M S
authors.initials: MS
authors.lastname: Pinner
authors.forename: H
authors.initials: H
authors.lastname: Brundish
authors.forename: P G
authors.initials: PG
authors.lastname: Markham
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004267:DNA Viruses / Q000145:classification / Q000235:genetics*; D004270:DNA, Circular / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D007303:Insect Vectors; D007630:Kenya; D008969:Molecular Sequence Data; D008897:Panicum / Q000382:microbiology*; D010942:Plant Viruses / Q000145:classification / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping
chemical_list: D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral