Changes in proteoglycan turnover in experimental canine osteoarthritic cartilage.
The metabolism of newly-synthesised and total ("resident") proteoglycans was examined in control and osteoarthritic cartilage explants obtained from an experimental model (Pond and Nuki, 1973) of canine osteoarthritis. The following findings were obtained: (i) Non-labelled proteoglycans extracted from normal cartilage with 4 M guanidine HCl showed two bands visualised by staining with toluidine blue. The electrophoretic mobilities of proteoglycans from osteoarthritic cartilage were unchanged but the relative abundance of the slower migrating band increased with time after surgery. (ii) There were qualitative differences in the proteoglycan breakdown products released into the medium of explant cultures of osteoarthritic compared with control cartilage. This was apparent for both labelled and total unlabelled proteoglycans. (iii) There were similarities in the electrophoretic mobilities of the major labelled and non-labelled proteoglycan breakdown products suggesting that total ("resident") proteoglycans and newly-formed proteoglycans were degraded by similar mechanisms. There were however some differences in the labelled and non-labelled proteoglycans, suggesting that the mechanisms of breakdown were not identical. (iv) Immunoblotting techniques showed differences in the distribution of various glycosaminoglycans in proteoglycan breakdown products from control compared with osteoarthritic cartilage explant cultures. (v) Monoclonal antibodies 7-D-4 and 3-B-3 (which recognise unusual native chondroitin sulphate epitopes) showed greatly increased expression on proteoglycans from osteoarthritic cartilage compared with controls.
Carney
S L
SL
Biochemistry Division, Kennedy Institute of Rheumatology, Hammersmith, London, UK.
Billingham
M E
ME
Caterson
B
B
Ratcliffe
A
A
Bayliss
M T
MT
Hardingham
T E
TE
Muir
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001665:Binding Sites; D002358:Cartilage, Articular / Q000378:metabolism*; D002809:Chondroitin Sulfates / Q000276:immunology / Q000378:metabolism; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism; D007632:Keratan Sulfate / Q000276:immunology / Q000378:metabolism; D010003:Osteoarthritis / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D006025:Glycosaminoglycans; D011509:Proteoglycans; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D007632:Keratan Sulfate