Effects of reactive oxygen intermediate scavengers on the antitoxoplasmic activity of activated macrophages.
Obioactin, Lonomycin A, muramyl dipeptide, and scavengers of hydrogen radicals and of singlet oxygen were used to study the participation of.OH and 1O2 in the killing of Toxoplasma in cultures of glycogen-induced peritoneal macrophages. Both the scavengers of OH (diazabicyclooctane and histidine) and those of 1O2 (mannitol and sodium benzoate) failed to inhibit the multiplication of Toxoplasma in macrophages that were incubated with either Obioactin, Lonomycin A, or MDP. The results of these experiments demonstrate the apparent lack of an inhibitory effect of.OH and 1O2 on the multiplication of Toxoplasma, whereas the scavengers alone inhibited the growth of the parasites.
Saito
A
A
Department of Veterinary Physiology and Protozoan Immunology, Obihiro University, Japan.
Igarashi
I
I
Miyahara
K
K
Venturini
C
C
Claveria
F G
FG
Hirose
T
T
Suzuki
N
N
Ono
K
K
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology; D019817:Benzoic Acid; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D016166:Free Radical Scavengers; D006639:Histidine / Q000494:pharmacology; D007136:Immunoglobulins; D007476:Ionophores / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000469:parasitology*; D008353:Mannitol / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009550:Nigericin / Q000031:analogs & derivatives / Q000494:pharmacology; D014122:Toxoplasma / Q000187:drug effects / Q000254:growth & development / Q000276:immunology*
D000890:Anti-Infective Agents; D001565:Benzoates; D003470:Culture Media; D016166:Free Radical Scavengers; D007136:Immunoglobulins; D007476:Ionophores; C041544:obioactin; D008353:Mannitol; D006639:Histidine; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C012410:lonomycin; D019817:Benzoic Acid; D009550:Nigericin