Selection of intraepithelial lymphocytes with CD8 alpha/alpha co-receptors by self-antigen in the murine gut.
We have studied T-cell receptor (TCR) and alpha/alpha CD8 expression in thymus-independent intraepithelial lymphocytes (TI IELs) from the gut of mice bearing transgenic (TG) TCR alpha beta specific for the male antigen, presented by H-2Db class I major histocompatibility complex (MHC) molecules. In contrast to TCR+ alpha beta cells differentiating in the thymus (from CD4+CD8+ precursors to CD4+CD8- or CD4-CD8+ progeny), TI IELs are not deleted by self-antigens, nor are they positively selected in the absence of the specific peptide. On the contrary, recognition of the antigen in the context of self-MHC is required for selection and granular differentiation of CD8+ TI IELs. Our results also show that, in contrast to the thymus, expression of the beta TG does not block expression of endogenous TCR gamma delta genes in TI IELs. The size of this gut IEL subpopulation and its difference in mechanisms of repertoire selection demonstrate the existence of a major extrathymic pathway of T-cell differentiation, the role of which remains to be elucidated.
Rocha
B
B
Institut National de la Santé et de la Recherche Médicale, Hôpital Necker-Enfants-Malades, Paris, France.
von Boehmer
H
H
Guy-Grand
D
D
D000818:Animals; D000911:Antibodies, Monoclonal; D016827:CD8 Antigens / Q000276:immunology*; D002454:Cell Differentiation; D004848:Epithelium / Q000276:immunology; D005260:Female; D006182:H-Y Antigen / Q000276:immunology; D007422:Intestines / Q000276:immunology*; D046911:Macromolecular Substances; D008285:Major Histocompatibility Complex; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D012727:Sex Characteristics; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D006182:H-Y Antigen; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell