title: Immunochemical studies of histone H5 from Halobatrachus didactylus.
abstract: 1. Histone H5 from Halobatrachus didactylus was isolated by using perchloric acid (PCA) extraction of fish liver nuclei and trichloroacetic acid (TCA) precipitation. 2. A polyclonal antiserum was generated by immunizing rabbits with the antigen purified from SDS-PAGE. 3. By immunofluorescence the serum stains erythrocyte nuclei from H. didactylus but it does not react with mammalian cells. 4. By Western blotting, the anti-H5 antibody reacts with the isolated antigen at high titers. 5. Digestion of histone H5 with pepsin and cyanogen bromide suggests that the epitopes are located in the globular and C-terminal region of the H5 molecule excluding the N-terminal.
authors.affiliation: Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Cadiz, Puerto Real, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Pendon
authors.forename: M C
authors.initials: MC
authors.lastname: Rendon
authors.forename: M C
authors.initials: MC
authors.lastname: Sarrasquete
authors.forename: M M
authors.initials: MM
authors.lastname: Valdivia
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004912:Erythrocytes / Q000737:chemistry / Q000502:physiology; D005399:Fishes; D005455:Fluorescent Antibody Technique; D006657:Histones / Q000276:immunology / Q000302:isolation & purification*; D008099:Liver / Q000737:chemistry
chemical_list: D006657:Histones