Reduction by clofibric acid of serum arachidonic acid in rats. Effect on the acyl composition of renal phospholipids.
Alterations induced by p-chlorophenoxyisobutyric acid (clofibric acid) in the composition of phosphatidylcholine and cholesterol esters in serum and their influence on the composition of phosphatidylcholine in the kidney were studied. Rats of different ages responded differently to the drug in terms of the levels of arachidonic acid (20:4) and linoleic acid (18:2) in the phosphatidylcholine and cholesterol esters in the serum. Administration of clofibric acid to 26-week-old rats for 2 weeks caused a marked decreased in the relative level of 20:4 in phosphatidylcholine and cholesterol esters in serum, whereas similar treatment of 6-week-old rats resulted in a reduction of 18:2 and, to a lesser extent, of 20:4 in serum lipids. The decrease in phosphatidylcholine that contained 20:4 in the serum of old rats was mainly due to a decrease in the concentration of stearoyl-arachidonoyl (18:0-20:4) species. The decrease in cholesterol arachidonate in serum caused by the treatment of old rats with clofibric acid seemed to be due to a reduction in the relative level of serum phosphatidylcholine containing 20:4. The marked reduction in serum lipids that contained 20:4 caused a decrease in the relative level of 20:4 in renal phospholipids, in particular, a decrease in the proportion of palmitoyl-arachidonoyl (16:0-20:4) and 18:0-20:4 phosphatidylcholine.
Kawashima
Y
Y
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Nishizawa
H
H
Kozuka
H
H
D000367:Age Factors; D000818:Animals; D016718:Arachidonic Acid / Q000097:blood*; D002788:Cholesterol Esters / Q000097:blood; D002995:Clofibric Acid / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000097:blood; D008297:Male; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000097:blood; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002788:Cholesterol Esters; D008041:Linoleic Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D016718:Arachidonic Acid; D002995:Clofibric Acid; D019787:Linoleic Acid