Electric organ polyamines and their effects on the acetylcholine receptor.
1. The electric organ of Torpedo nobiliana contained putrescine (PUT), spermidine (SPD), spermine (SPM), and cadaverine (CAD). Traces of acetylated SPD and SPM were occasionaly seen. 2. Upon fractionation of the tissue by differential centrifugation, the polyamines (PA) were found predominantly in the soluble fraction. The postsynaptic membrane fraction, containing a high concentration of acetylcholine receptor (AChR), was proportionally enriched in SPM. The molar ratio of SPM to AChR was approximately two in these membranes. 3. The effect of exogeneous PA on AChR function was studied by two methods: carbamoylcholine (CCh)-dependent 86Rb+ influx into receptor-rich membrane vesicles and [alpha-125I]bungarotoxin (Bgt) binding to the AChR. 4. SPM inhibited both ion influx and the rate of Bgt binding at concentrations above 1 mM, and therefore it appears to act as a competitive antagonist of the AChR. 5. At submicromolar concentrations, and only after preincubation with the receptor-rich membrane, SPM and PUT increased the ion influx by about 20% over control values. 6. Preincubation with 100 nM SPM did not affect the equilibrium binding of iodinated toxin or the rate of toxin binding, and therefore SPM was not uncovering new receptors. 7. By measuring the initial rate of toxin binding after different periods of preincubation with 1 microM CCh, the rate of the slow phase of receptor desensitization was determined. This rate was not changed by 100 nM SPM. 8. Although these results suggest that at low concentrations SPM is a positive modulator of the AChR, the precise mechanism of action is not determined yet.
Szczawinska
K
K
Department of Biochemistry, Universidad Central del Caribe, Bayamon, Puerto Rico.
Ferchmin
P A
PA
Hann
R M
RM
Eterović
V A
VA
D000818:Animals; D001667:Binding, Competitive; D002038:Bungarotoxins / Q000378:metabolism / Q000494:pharmacology; D002217:Carbachol / Q000378:metabolism / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry; D004557:Electric Organ / Q000737:chemistry*; D011073:Polyamines / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000187:drug effects* / Q000378:metabolism; D012413:Rubidium / Q000378:metabolism; D013096:Spermine / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D014101:Torpedo / Q000378:metabolism*
D002038:Bungarotoxins; D011073:Polyamines; D011950:Receptors, Cholinergic; D013096:Spermine; D002217:Carbachol; D012413:Rubidium