Initiating activity of 1,1,2,2-tetrachloroethane in two-stage BALB/c 3T3 cell transformation.
By using in vitro two-stage BALB/c 3T3 cell transformation assay, we have tested the effect of promoting treatment with tetradecanoylphorbol acetate (TPA) on transformation induced by 1,1,2,2-tetrachloroethane (1,1,2,2-TTCE). Cells were treated with subeffective or transforming concentrations of 1,1,2,2-TTCE in the presence of an S9-mix activating system, followed by TPA promoting treatment. The transforming activity of 1,1,2,2-TTCE is evident only by reseeding confluent cells and allowing additional rounds of cell replications in the amplification test. Treatment with TPA leads to a marked transformation yield in all plates scored even at the lowest assayed dosage of 1,1,2,2-TTCE, without performing amplification of transformation.
Colacci
A
A
Istituto Nazionale per la Ricerca sul Cancro (IST-Genova), Biotechnology Satellite Unit-Bologna, Italy.
Perocco
P
P
Bartoli
S
S
Da Via
C
C
Silingardi
P
P
Vaccari
M
M
Grilli
S
S
D016475:3T3 Cells / Q000187:drug effects / Q000648:ultrastructure; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D004305:Dose-Response Relationship, Drug; D004980:Ethane / Q000031:analogs & derivatives* / Q000633:toxicity; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D008110:Liver Extracts / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D006843:Hydrocarbons, Chlorinated; D008110:Liver Extracts; C015530:1,1,2,2-tetrachloroethane; D004980:Ethane; D013755:Tetradecanoylphorbol Acetate