Presence and specific concentration of carbonic anhydrase II in matrix vesicles.
Matrix vesicles were isolated from the epiphyseal growth plates of normal weanling rats, and the presence of carbonic anhydrase II was demonstrated by Western blotting and ultrastructural immunolocalization using the immunogold technique. Total carbonic anhydrase activity was assayed and showed a statistically significant increase in matrix vesicles as compared to normal rat chondrocytes derived from the same growth plates. These results are the first to establish the presence of carbonic anhydrase in matrix vesicles.
Stechschulte
D J
DJ
Department of Pathology, University of Kansas Medical Center, Kansas City.
Morris
D C
DC
Silverton
S F
SF
Anderson
H C
HC
Väänänen
H K
HK
D000818:Animals; D001857:Bone Matrix / Q000201:enzymology* / Q000648:ultrastructure; D002113:Calcification, Physiologic; D002256:Carbonic Anhydrases / Q000378:metabolism*; D006132:Growth Plate / Q000201:enzymology / Q000648:ultrastructure; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D051381:Rats
D002256:Carbonic Anhydrases