An enzyme-linked immunosorbent assay for the detection of plasminogen activator-induced fribrin clot lysis in a circulating plasma system.
A technically easy and sensitive method for the evaluation of the fibrinolytic potency of plasminogen activator in a circulating plasma system was developed. A circulating apparatus was prepared by connecting silicone tubes with two separate chambers. Human plasma and plasminogen activator were allowed to circulate from one chamber through a peristaltic pump into another one which contained biotin-labeled fibrin clot. The extent of fibrinolysis was evaluated on streptavidin-coated microtiter plates by measuring the amount of the avidin-bound fibrin degradation products with peroxidase-linked antibody. The detection limit of the fibrinolytic products was 1.5 ng/ml. The present method dose not require any specialized equipment and is also applicable to a low-cost routine measurement of the thrombus selectivity of plasminogen activator.
Soeda
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
Sakaguchi
S
S
Shimeno
H
H
Nagamatsu
A
A
D001426:Bacterial Proteins / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005342:Fibrinolysis; D006801:Humans; D019809:Streptavidin; D010959:Tissue Plasminogen Activator / Q000494:pharmacology*
D001426:Bacterial Proteins; D001710:Biotin; D019809:Streptavidin; D010959:Tissue Plasminogen Activator