Preliminary crystallographic analysis of murine macrophage inflammatory protein 2.
Macrophage inflammatory protein 2 (MIP-2) has been crystallized by vapor diffusion of an 11 mg/ml protein solution in 100 mM-ammonium acetate against 30 to 40% polyethylene glycol (average molecular mass of 3350 Da). The crystals belong to space group P2(1)2(1)2(1) and have unit cell dimensions of a = 42.7 A, b = 59.3 A, and c = 100.3 A. The molecular mass of the protein and volume of the unit cell suggest that there are four monomers in the asymmetric unit. A data set to 2.3 A has been collected, and the self-rotation function identifies the presence of a non-crystallographic 2-fold axis.
Lolis
E
E
Laboratory of Medical Biochemistry, Rockefeller University, New York, NY 10021.
Sweet
R M
RM
Cousens
L S
LS
Tekamp-Olson
P
P
Sherry
B A
BA
Cerami
A
A
D000818:Animals; D054426:Chemokine CXCL2; D003461:Crystallography; D016207:Cytokines / Q000648:ultrastructure*; D051379:Mice; D015846:Monokines / Q000648:ultrastructure*; D011487:Protein Conformation; D011994:Recombinant Proteins
D054426:Chemokine CXCL2; D016207:Cytokines; D015846:Monokines; D011994:Recombinant Proteins