Production and characterization of an iminoimidazolidine specific monoclonal antibody using para-aminoclonidine as antigen.
Para-aminoclonidine coupled to hemocyanin was used to produce mouse monoclonal antibodies directed against clonidine. The properties of one of these, called mFE7, secreted by a clone of hybrid myeloma, are described. This antibody displayed total crossreactivity with imidazolidines and no crossreactivity at all with catecholamines or other known naturally occurring substances tested. A liquid phase radioimmunoassay permitted the detection of immunoreactivity in human brain extracts. The mFE7 antibody could be useful for immunopurifying the endogenous ligand of Imidazolines Preferring Receptors (IPR) which are catecholamines insensitive.
Dontenwill
M
M
CNRS URA 589, Faculté de Médecine, Université Louis Pasteur, Strasbourg, France.
Molines
A
A
Bricca
G
G
Stutzman
J
J
Kempf
J
J
Belcourt
A
A
Bousquet
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions / Q000502:physiology; D000941:Antigens / Q000276:immunology* / Q000378:metabolism; D001667:Binding, Competitive; D001921:Brain / Q000276:immunology; D003000:Clonidine / Q000031:analogs & derivatives* / Q000276:immunology / Q000378:metabolism; D005260:Female; D005976:Glutaral / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D007093:Imidazoles / Q000276:immunology* / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011224:Prazosin / Q000494:pharmacology; D014020:Tissue Extracts / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology / Q000378:metabolism; D015016:Yohimbine / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000941:Antigens; D007093:Imidazoles; D014020:Tissue Extracts; D014614:Vaccines, Synthetic; D015016:Yohimbine; C016986:apraclonidine; D006433:Hemocyanins; D003000:Clonidine; D005976:Glutaral; D011224:Prazosin