Immunochemical studies of histone H5 from Halobatrachus didactylus.
1. Histone H5 from Halobatrachus didactylus was isolated by using perchloric acid (PCA) extraction of fish liver nuclei and trichloroacetic acid (TCA) precipitation. 2. A polyclonal antiserum was generated by immunizing rabbits with the antigen purified from SDS-PAGE. 3. By immunofluorescence the serum stains erythrocyte nuclei from H. didactylus but it does not react with mammalian cells. 4. By Western blotting, the anti-H5 antibody reacts with the isolated antigen at high titers. 5. Digestion of histone H5 with pepsin and cyanogen bromide suggests that the epitopes are located in the globular and C-terminal region of the H5 molecule excluding the N-terminal.
Pendon
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Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Cadiz, Puerto Real, Spain.
Rendon
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MC
Sarrasquete
M C
MC
Valdivia
M M
MM
D000818:Animals; D015153:Blotting, Western; D004912:Erythrocytes / Q000737:chemistry / Q000502:physiology; D005399:Fishes; D005455:Fluorescent Antibody Technique; D006657:Histones / Q000276:immunology / Q000302:isolation & purification*; D008099:Liver / Q000737:chemistry
D006657:Histones