Adsorption profile of commercially available adsorbents: an in vitro evaluation.
Adsorbents from four commercially available devices, Protein A-Sepharose (Immunosorba Protein A-62,5; Excorim KB, Lund Sweden), Tryptophan-PVA (Immusorba TR-350; Asahi Medical Co., Tokyo, Japan), Phenylalanine-PVA (Immusorba PH-350; Asahi Medical Co., Tokyo, Japan), and Dextran sulfate (Liposorber LA-15; Kanegafuchi Chemical Co. Ltd, Osaka, Japan) were tested under optimal in vitro conditions to determine their adsorption capability for several plasma constituents which are usually the target of plasma therapy. The parameters of interest were: double stranded DNA-antibodies (anti-dsDNA), antiglomerular basement membrane antibodies (anti-GBM), anti-acetylcholin receptor antibodies (AChRAb), circulating immune complexes (CIC), rheumatoid factor (RF), IgA, IgG, IgM, IgE, C3c, C4, LDL-cholesterol, total cholesterol, erythropoietin (EPO) and beta 2-microglobulin (beta 2M). The IgG auto antibodies, CIC and RF can be removed by Protein A-Sepharose, Try-PVA and Phe-PVA. IgG is best adsorbed by Protein A-Sepharose, while IgE can be removed efficiently by Try-PVA. Dextran sulfate is without doubt the best adsorbent for LDL-cholesterol. All four adsorbents bind also complement components C3c and C4. No significant adsorption was found for EPO and beta 2M. The four devices exhibit a quite different adsorption profile which can be used as a guide for the optimal selection of an adsorption column in clinical apheresis.
Ikonomov
V
V
Nephrology Department, Klinikum Grosshadern, University of Munich, Germany.
Samtleben
W
W
Schmidt
B
B
Blumenstein
M
M
Gurland
H J
HJ
D000327:Adsorption; D000906:Antibodies / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003181:Complement C4 / Q000302:isolation & purification; D016264:Dextran Sulfate / Q000097:blood; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008422:Materials Testing; D010649:Phenylalanine / Q000097:blood; D010951:Plasma Exchange / Q000379:methods*; D011950:Receptors, Cholinergic / Q000302:isolation & purification; D013205:Staphylococcal Protein A / Q000097:blood; D014364:Tryptophan / Q000097:blood
D000906:Antibodies; D008078:Cholesterol, LDL; D003181:Complement C4; D007074:Immunoglobulin G; D011950:Receptors, Cholinergic; D013205:Staphylococcal Protein A; D010649:Phenylalanine; D014364:Tryptophan; D016264:Dextran Sulfate