Use of a commercial double-test tablet (Rosco PGUA/indole) for screening of Escherichia coli.
A commercial double-test tablet (Rosco PGUA/indole) for detection of beta-glucuronidase (beta-GUR) activity and indole production was evaluated on a collection of 393 isolates of Enterobacteria. Both beta-GUR and indole were positive on 96.6% of Escherichia coli strains. beta-GUR, only, was also detected in 25 Shigella spp., four Enterobacter cloacae, eight Citrobacter freundii, and five Salmonella enteritidis strains, none of which were indole producers. An additional 261 consecutive clinical isolates of oxidase-negative nonswarming Gram-negative bacilli were studied in a parallel comparative field trial against conventional identification methods. For 200 strains, the standard method and PGUA/indole test were performed from the primary culture plate. The remaining 61 (23.4%) required subculture before testing. Sensitivity, specificity, positive predictive value, and negative predictive value of PGUA/indole test in the screening for E. coli were, respectively, 94.1%, 100%, 100%, and 87.1%. In our experience, PGUA/indole test is a rapid, precise, simple-to-perform, and economical method for screening E. coli. However, the need for a large inoculum may limit its application on primary cultures.
Domínguez
A
A
Service of Microbiology, Hospital de Bellvitge Príncipes de España, Barcelona, Spain.
Alcaide
F
F
Pulido
A
A
Ayats
J
J
Pérez
J L
JL
Martín
R
R
D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology / Q000302:isolation & purification*; D005966:Glucuronidase / Q000032:analysis*; D007211:Indoles / Q000032:analysis*
D007211:Indoles; C030374:indole; D005966:Glucuronidase