title: Characterization of ATP-diphosphohydrolase activities in the intima and media of the bovine aorta: evidence for a regulatory role in platelet activation in vitro.
abstract: The inner layer of the aorta contains the enzyme ATP diphosphohydrolase (ATPDase: EC 3.6.1.5) which catalyzes the sequential phosphorolysis of ATP----ADP----AMP. Two zones of the inner layer, the intima and media, were separated and both were shown to contain ATPDase activity of similar specific activity (0.08 and 0.10 U/mg protein, respectively). However, the media exhibited about 100-times more enzyme activity than the intima. Both preparations were virtually identical with respect to pH optima (7.5), migration patterns after electrophoresis under non-denaturing conditions, relative rates of ATP and ADP hydrolysis and potency to inhibit ADP-induced platelet aggregation in both human platelet-rich plasma and whole blood. The IC50 values for ADP (2 microM)-induced aggregation were 6.8 and 12.9 mU/ml in platelet-rich plasma and whole blood, respectively. Addition of ATPDase to platelets pre-aggregated with ADP resulted in a dose-dependent disaggregation in platelet-rich plasma (IC50 4.9 mU/ml), but not in whole blood. When both ATPDase (5.6-58.7 mU/ml) and ATP (0.5-10 microM) were added to platelet-rich plasma, there was an immediate dose-dependent aggregation of platelets followed by a slowly developing disaggregation. These data show that ATPDase is present in both the intima and media layers of bovine aorta and suggest a dual role for this enzyme in platelet activation. By converting ATP released from damaged cells into ADP, the enzyme could facilitate platelet aggregation at the site of vascular injury, whereas the subsequent conversion of ADP to AMP could inhibit or reverse platelet aggregation. The consequence of these activities would be to control the growth of a platelet thrombus.
authors.affiliation: Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Québec, Canada.
authors.forename: Y P
authors.initials: YP
authors.lastname: Côté
authors.forename: J G
authors.initials: JG
authors.lastname: Filep
authors.forename: B
authors.initials: B
authors.lastname: Battistini
authors.forename: J
authors.initials: J
authors.lastname: Gauvreau
authors.forename: P
authors.initials: P
authors.lastname: Sirois
authors.forename: A R
authors.initials: AR
authors.lastname: Beaudoin
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000328:Adult; D000818:Animals; D001011:Aorta / Q000033:anatomy & histology / Q000201:enzymology* / Q000378:metabolism; D001081:Apyrase / Q000378:metabolism* / Q000494:pharmacology; D002417:Cattle; D004730:Endothelium, Vascular / Q000033:anatomy & histology / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D015539:Platelet Activation; D010974:Platelet Aggregation
chemical_list: D000255:Adenosine Triphosphate; D001081:Apyrase