title: Ferritin mRNAs on rat liver membrane-bound polysomes synthesize ferritin that does not translocate across membranes.
abstract: Ferritin is a typical intracellular protein but small amounts are also present in serum and other biological fluids. The source and physiological significance of serum ferritin are still obscure. The presence of ferritin mRNAs on polysomes bound to endoplasmic reticulum (ER) could be relevant for the secretion of ferritin. By Northern blot analysis we found significant amounts of both L and H subunit mRNAs on rat liver membrane-bound polysomes. Immunoprecipitation of translational products of membrane-bound polysomes with anti-rat liver ferritin antibody showed that ferritin is actually synthesized on ER membranes. Analysis of RNA extracted from salt-washed rat liver microsomes demonstrated that ferritin mRNAs are translated by polysomes tightly bound to ER membranes. Following iron treatment, both the amount of H and L subunit mRNAs and ferritin synthesis increased sharply in both free and bound polysomal fractions. Translation of membrane-bound polysomes in the presence of microsomal membranes indicated that ferritin is not processed by signal sequence cleavage or glycosylation and is not translocated into ER membranes. Ferritin mRNAs found on membrane-bound polysomes are associated with ER in a specific way, however, their products do not seem to follow the classic secretory pathway and therefore the significance of the large amount of ferritin mRNAs in the bound ribosome fraction remains unclear.
authors.affiliation: Istituto di Patologia Generale, Università degli Studi, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Tacchini
authors.forename: E
authors.initials: E
authors.lastname: Rappocciolo
authors.forename: M
authors.initials: M
authors.lastname: Ferrero
authors.forename: L
authors.initials: L
authors.lastname: Schiaffonati
authors.forename: G
authors.initials: G
authors.lastname: Cairo
mesh_terms: D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005293:Ferritins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D007501:Iron / Q000494:pharmacology; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D011132:Polyribosomes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride
chemical_list: D012333:RNA, Messenger; D012965:Sodium Chloride; D005293:Ferritins; D007501:Iron