Effects of chloral, chloralose and pentobarbitone on monoamine and (neuropeptide Y) NPY levels in various tissues in the rat.
Sampling of blood or tissues in experimental animals need frequently a general anesthesia. Some anesthetic agents are known to modify plasma levels of catecholamines; moreover NPY is co-released with noradrenaline or adrenaline under certain conditions. Thus, we investigated the effect of three general anesthetic agents, chloral, chloralose and pentobarbital (pentobarbitone) on the levels of monoamines and NPY in rat hypothalamus, medulla, atria and adrenals. Monoamines were analysed by high pressure liquid chromatography using an electrochemical detection and NPY was measured by radioimmunoassay (immunoradiometric assay, IRMA). Chloralose or pentobarbitone did not affect monoamine tissue levels. In contrast, chloral increased 5-hydroxyindolacetic acid and to a lesser extent serotonin levels in the hypothalamus. NPY tissue levels did not change whatever the anesthetic used. These results must be taken into account in pharmacological or toxicological studies which need the use of a general anesthesia.
Heimburger
M
M
Laboratories de Toxicologie, Faculté de Pharmacie, Châtenay Malabry.
Pages
N
N
Davy
M
M
el Rawadi
C
C
Cohen
Y
Y
Bohuon
C
C
D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D002395:Catecholamines / Q000378:metabolism; D002697:Chloral Hydrate / Q000031:analogs & derivatives / Q000494:pharmacology; D002698:Chloralose / Q000494:pharmacology; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism*; D010424:Pentobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D000777:Anesthetics; D015306:Biogenic Monoamines; D002395:Catecholamines; D007211:Indoles; D009478:Neuropeptide Y; D002698:Chloralose; D002697:Chloral Hydrate; C021100:trichloroacetaldehyde; D010424:Pentobarbital