[Characterization of two novel cell lines established from a single human uterine cervical adenocarcinoma, and their chemosensitivities and hormonal effects on their proliferation].
Two new cell lines were established from a single human uterine cervical adenocarcinoma which had been successfully transplanted into nude mice. They were subcultivated more than 70 times over a period of 46 months and designated as TCO-1 and TCO-2, respectively. Growth characteristics, modes of chromosomes and specific tumor makers were different in TCO-1 and TCO-2. The plating efficiency of TCO-1 was greater and its doubling time was shorter than those of TCO-2. Modes of chromosomes were 77 in TCO-1 and 94 in TCO-2. Their chemosensitivities to 13 anticancer drugs were studied by MTT assay. Only the mitomycin C (MMC) was an effective agent for both TCO-1 and TCO-2 cell lines which were characteristically different from each other. The results suggest that MMC would be a promising agent for chemotherapy of human uterine cervical adenocarcinoma. The influences of 6 hormones (estradiol, progesterone, dihydrotestosterone, medroxyprogesterone acetate, human menopausal gonadotropin, human chorionic gonadotropin) on their proliferation were studied by MTT assay and dye exclusion assay. TCO-1 and TCO-2 showed no significant changes in proliferation in any of them. It is suggested that hormone replacement therapy could be indicated even for postoperative patients with cervical adenocarcinoma.
Fushiki
H
H
Department of Obstetrics and Gynecology, Faculty of Medicine, Toyama Medical and Pharmaceutical University.
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D002225:Carbazilquinone / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D004958:Estradiol / Q000494:pharmacology; D005047:Etoposide / Q000494:pharmacology; D005260:Female; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology*; D051379:Mice; D016685:Mitomycin / Q000494:pharmacology*; D009368:Neoplasm Transplantation; D011374:Progesterone / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
D002225:Carbazilquinone; D011374:Progesterone; D004958:Estradiol; D016685:Mitomycin; D005047:Etoposide; D008727:Methotrexate