A sensitive and specific high performance liquid chromatographic assay for imidazole dipeptides and 3-methylhistidine in human muscle biopsies, serum and urine.
A sensitive and specific high performance liquid chromatographic method is described for measuring imidazole dipeptides and 3-methylhistidine in human muscle biopsies, serum and urine. Muscle extract, serum or urine was reacted with o-phthaldialdehyde and the derivatives were separated by reversed phase chromatography with column switching and fluorescence detection.
Teahon
K
K
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
Rideout
J M
JM
D000861:Anserine / Q000097:blood / Q000378:metabolism* / Q000652:urine; D001706:Biopsy; D002336:Carnosine / Q000097:blood / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008762:Methylhistidines / Q000097:blood / Q000378:metabolism* / Q000652:urine; D009132:Muscles / Q000737:chemistry* / Q000473:pathology; D013050:Spectrometry, Fluorescence
D008762:Methylhistidines; D002336:Carnosine; D000861:Anserine; C028118:3-methylhistidine