Antitumor activity of Toxoplasma lysate antigen against methylcholanthrene-induced tumor-bearing rats.
Growth of the tumor autoinduced by 20-methylcholanthrene (MC) in rats was inhibited after administration of Toxoplasma lysate antigen (TLA). The antitumor activity of TLA was most obvious in the early stage of tumoral growth. When TLA was administered to rats before the appearance of tumour, tumor formation was delayed slightly. Histopathological studies revealed dense growths of spindle tumor cells in untreated control rat, while enlarged central necrosis with the infiltration of lymphocytes and neutrophils was apparent in TLA-treated rats. According to the immunohistological examination of tumor tissue with anti-Thy-1 antibody, the rats treated with TLA showed large Thy-1 positive granular cells, whereas the untreated rats indicated only a few small Thy-1 positive cells. These observations indicate that TLA is a useful modifier of biological responses to MC-induced tumors.
Miyahara
K
K
Department of Veterinary Clinical Radiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Yokoo
N
N
Sakurai
H
H
Igarashi
I
I
Sakata
Y
Y
Yoshida
Y
Y
Saito
A
A
Hirose
T
T
Suzuki
N
N
D000818:Animals; D000953:Antigens, Protozoan / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D005354:Fibrosarcoma / Q000628:therapy; D007150:Immunohistochemistry; D007155:Immunologic Factors / Q000627:therapeutic use*; D008297:Male; D008748:Methylcholanthrene; D009374:Neoplasms, Experimental / Q000628:therapy*; D051381:Rats; D011919:Rats, Inbred Strains; D014122:Toxoplasma / Q000276:immunology*
D000953:Antigens, Protozoan; D007155:Immunologic Factors; D008748:Methylcholanthrene