Effect of respiratory syncytial virus on adherence, colonization and immunity of non-typable Haemophilus influenzae: implications for otitis media.
Adherence of non-typable Haemophilus influenzae to respiratory epithelium was evaluated in a cotton rat model of respiratory syncytial virus (RSV) infection. Colonization with non-typable H. influenzae increased to a maximum within 4 days of RSV infection compared to RSV negative controls (4.58 +/- 0.17 vs 3.82 +/- 0.23 log colony forming units (CFU) per ml, P less than 0.05) and then declined over the subsequent 10 days (2.0 +/- 0 vs 3.78 +/- 0.39 CFU per ml, P less than 0.0001). In a second series of experiments, attachment of non-typable H. influenzae to epithelial cells collected from RSV infected cotton rats at the time of maximum virus replication was not different from controls (57.4 +/- 18.3 vs 52.0 +/- 24.3 bacteria per 50 cells). Systemic immunity to non-typable H. influenzae as measured by IgG-specific antibody to the outer membrane complex and bactericidal antibody did not influence colonization. These data suggest that colonization with non-typable H. influenzae is significantly affected by a concurrent infection with RSV; however, the site of bacterial attachment is not known.
Patel
J
J
Department of Pediatrics, State University of New York School of Medicine, Buffalo.
Faden
H
H
Sharma
S
S
Ogra
P L
PL
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis; D001422:Bacterial Adhesion / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D015169:Colony Count, Microbial; D005260:Female; D006193:Haemophilus influenzae / Q000145:classification / Q000276:immunology* / Q000502:physiology*; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D009305:Nasopharynx / Q000382:microbiology; D010033:Otitis Media / Q000382:microbiology*; D012136:Respiratory Syncytial Viruses / Q000276:immunology / Q000502:physiology*; D010253:Respirovirus Infections / Q000503:physiopathology*; D016205:Sigmodontinae
D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G