title: Selective inhibition of membrane fusion events in echinoderm gametes and embryos by halenaquinol sulfate.
abstract: Halenaquinol sulfate, a hydroquinone sulfate obtained from the sponge Xestospongia sapra, prevented cell membrane fusion events of echinaderm gametes but did not affect early embryonic development of fertilized eggs up to the gastrula stage. However, halenaquinol sulfate inhibited secretion of hatching enzyme, resulting in the formation of gastrulae that were surrounded by the fertilization envelope. Therefore, the use of halenaquinol sulfate offers a unique opportunity to analyze the role of secretory events in complex populations of cells without affecting other cellular functions.
authors.affiliation: Department of Applied Biochemistry, Hiroshima University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ikegami
authors.forename: N
authors.initials: N
authors.lastname: Kajiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Ozaki
authors.forename: Y
authors.initials: Y
authors.lastname: Myotoishi
authors.forename: S
authors.initials: S
authors.lastname: Miyashiro
authors.forename: S
authors.initials: S
authors.lastname: Takayama
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: I
authors.initials: I
authors.lastname: Kitagawa
mesh_terms: D000818:Animals; D001551:Benz(a)Anthracenes / Q000494:pharmacology*; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D004447:Echinodermata / Q000196:embryology*; D005306:Fertilization / Q000187:drug effects / Q000502:physiology; D005775:Gastrula / Q000187:drug effects / Q000502:physiology; D008297:Male; D008561:Membrane Fusion / Q000187:drug effects*; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D001551:Benz(a)Anthracenes; C074982:halenaquinol sulfate