title: Production of 12-hydroxyeicosatetraenoic acid in early pregnant uterine cervix--lack of correlation to mifepristone-induced cervical ripening. A double-blind randomized biomechanical and biochemical study.
abstract: The regulation of cervical ripening in pregnancy may involve arachidonic acid metabolites. We studied the formation of lipoxygenase products in cervical biopsies from twenty nulliparous women requesting a first trimester abortion. The patients were randomly allocated to receive either 100 mg of the progesterone antagonist mifepristone (RU 486) or placebo 48 and 36 hours before surgery. A capacity to produce significant amounts of 12-HETE and material co-chromatographing with leukotrienes was observed in the cervical tissue. No qualitative or quantitative relationship to mifepristone-induced cervical ripening was found. Although our data suggest a large variation of 12-HETE production it remains to clarify its role in the cervix.
authors.affiliation: Department of Physiological Chemistry, Karolinska Institute, Stockholm, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Heidvall
authors.forename: A
authors.initials: A
authors.lastname: Radestad
authors.forename: N J
authors.initials: NJ
authors.lastname: Christensen
authors.forename: J A
authors.initials: JA
authors.lastname: Lindgren
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000028:Abortion, Induced; D000328:Adult; D002584:Cervix Uteri / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008084:Lipoxygenase / Q000378:metabolism; D015735:Mifepristone / Q000494:pharmacology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000378:metabolism*
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D015735:Mifepristone; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D008084:Lipoxygenase