Bio-analysis of vinorelbine by high-performance liquid chromatography with fluorescence detection.
A simple and selective procedure for the determination of vinorelbine, a new semi-synthetic vinca alkaloid, is presented. The method is based on ion-exchange high-performance liquid chromatography on normal-phase silica with fluorescence detection, combined with liquid-liquid extraction using diethyl ether for sample clean-up. The absence of endogenous interferences and the excellent chromatographic behaviour of vinca alkaloids provides accurate results even at low concentrations. The limit of determination in plasma is 1.5 micrograms/l (500-microliters sample). Reproducible recoveries in urine were obtained if 10-50 microliters of sample were processed supplemented with 500 microliters of blank plasma.
van Tellingen
O
O
Department of Clinical Chemistry, Netherlands Cancer Institute, Antoni van Leeuwenhoekhuis (AvL), Amsterdam.
Kuijpers
A
A
Beijnen
J H
JH
Baselier
M R
MR
Burghouts
J T
JT
Nooyen
W J
WJ
D000970:Antineoplastic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000097:blood; D000077235:Vinorelbine
D000970:Antineoplastic Agents; D014747:Vinblastine; D000077235:Vinorelbine