The P gene of the porcine paramyxovirus LPMV encodes three possible polypeptides P, V and C: the P protein mRNA is edited.
The nucleotide sequence of the P gene of the porcine paramyxovirus La-Piedad-Michoacan-Mexico virus (LPMV) was analysed. Three long open reading frames (ORFs) were found in the mRNA sense. Insertion of two G residues is necessary to obtain an ORF encoding the P protein, which gives a P protein of 404 amino acids with a calculated Mr of 42475. This form of editing was demonstrated, two non-templated G residues being added in a portion of the mRNA transcripts. The LPMV V protein, which has a conserved cysteine-rich C-terminal region, is encoded by an exact copy of the P gene. The third ORF has the capacity to encode a protein of 126 amino acids, which may resemble the C proteins found in some paramyxoviruses. The ORF starts from an AUG codon down-stream of the first AUG codon of the P/V ORF.
Berg
M
M
Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala.
Hjertner
B
B
Moreno-López
J
J
Linné
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D010252:Paramyxoviridae / Q000235:genetics*; D010750:Phosphoproteins; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism; D016364:Reading Frames / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014764:Viral Proteins / Q000235:genetics*
C055859:P protein, Sendai virus; D010750:Phosphoproteins; D012333:RNA, Messenger; D014764:Viral Proteins; C074575:C protein, Porcine rubulavirus; C074576:V protein, Porcine rubulavirus