Virulence-associated 15- to 17-kilodalton antigens in Rhodococcus equi: temperature-dependent expression and location of the antigens.
Virulent Rhodococcus equi showing 15- to 17-kDa antigens, which is virulent in mice, was found to harbor an 85-kbp plasmid, and the 15- to 17-kDa antigens were found to be associated with possession of the 85-kbp plasmid of R. equi (S. Takai, T. Sekizaki, T. Ozawa, T. Sugawara, Y. Watanabe, and S. Tsubaki, Infect. Immun. 59:4056-4060, 1991). The expression of these antigens was temperature regulated: when cells were grown at a low temperature (25 to 32 degrees C), they did not express them, whereas they expressed them in large amounts when the cells were grown at a higher temperature (34 to 41 degrees C). The antigens were expressed on the cell surface, as evidenced by their susceptibility to proteolysis by a trypsin and by the biotin-avidin protein-blotting technique.
Takai
S
S
Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Iie
M
M
Watanabe
Y
Y
Tsubaki
S
S
Sekizaki
T
T
D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000096:biosynthesis / Q000302:isolation & purification; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D006358:Hot Temperature / Q000009:adverse effects; D016929:Rhodococcus equi / Q000276:immunology* / Q000472:pathogenicity*; D014357:Trypsin / Q000494:pharmacology; D014774:Virulence / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D014357:Trypsin