title: Thyroid stimulating hormone enhancement of bovine oocyte maturation in vitro.
abstract: Efforts to improve bovine embryonic development in vitro involved study of effects of thyroid stimulating hormone (TSH) alone or in combination with LH on bovine oocyte maturation (IVM). Putative effects were assessed by observing cumulus expansion (CE), fertilization (IVF), and development to morulae/blastocysts (M/B). Effects of prolactin (PRL) were also investigated. Variables for the 24-hr IVM interval were no hormone (control), TSH (0.1, 0.5, or 1.0 micrograms/ml) or PRL (10, 100, or 1000 micrograms/ml), luteinizing hormone (LH) (0, 10, or 100 micrograms/ml) + TSH (0.1 or 0.5 micrograms/ml), and serum (20%, v/v) + 0.5 micrograms TSH/ml; data were from 4-5 trials for each IVM treatment. Higher proportions of oocytes exhibited complete CE with hormones or serum than without (P less than 0.05). All oocytes (with and without CE) were inseminated with heparin-capacitated sperm. A higher proportion of inseminated oocytes cleaved after IVM with 0.5 micrograms TSH/ml (53.4%) than for other TSH treatments (P less than 0.05). The combination of TSH (0.1 and 0.5 micrograms/ml) with 10 micrograms LH/ml for IVM enabled higher proportions (P less than 0.05) of ova to fertilize (67.4 and 69.2%) than did medium alone (28.3%), LH (10 micrograms/ml) alone (54.1%) or serum + 0.5 micrograms TSH/ml (55.6%). No improvement in proportions undergoing fertilization was seen after addition of TSH to 100 micrograms LH/ml for IVM. Frequency of CE and cleavage did not differ among PRL treatments. More M/B developed from cleaved ova after IVM with LH or TSH than with PRL or no hormone (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens 30602.
authors.forename: A I
authors.initials: AI
authors.lastname: Younis
authors.forename: B G
authors.initials: BG
authors.lastname: Brackett
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005306:Fertilization / Q000502:physiology; D005307:Fertilization in Vitro; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*; D009866:Oogenesis / Q000187:drug effects*; D011388:Prolactin / Q000494:pharmacology; D013094:Spermatozoa; D013972:Thyrotropin / Q000494:pharmacology*
chemical_list: D011388:Prolactin; D007986:Luteinizing Hormone; D013972:Thyrotropin