title: Enhancement of optokinetic and vestibuloocular responses in the rabbit by cholinergic stimulation of the flocculus.
abstract: Bilateral microinjections into the cerebellar flocculus of the rabbit of carbachol, a general cholinergic agonist, profoundly affect vestibuloocular (VOR) and optokinetic (OKR) reflexes. For sinusoidal stimuli (0.15 Hz, 5 deg peak to peak), the gain of the OKR was strongly increased, while the gain of the VOR was moderately increased. These effects were partially mimicked by floccular injection of the acetylcholinesterase inhibitor eserine. Floccular injection of the muscarinic blocker atropine significantly lowered the gain of the OKR. The effects of the nicotinic blocker mecamylamine were not significant. Optokinetic nystagmus (OKN) in response to constant stimulus velocities (1-30 deg/second) showed a markedly accelerated buildup and a shortened optokinetic after-nystagmus (OKAN) after floccular injections of carbachol. The steady-state gain of OKN remained unaffected. None of the described effects occurred after floccular injection of the solvent, saline. It is postulated that cholinergic cerebellar afferents, one probable source of which are the vestibular nuclei, enhance the optokinetic and vestibular modulation of floccular Purkinje cells.
authors.affiliation: Department of Physiology I, Faculty of Medicine, Erasmus University Rotterdam, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Collewijn
authors.forename: H S
authors.initials: HS
authors.lastname: Tan
authors.forename: J
authors.initials: J
authors.lastname: Van der Steen
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D002217:Carbachol / Q000494:pharmacology*; D002531:Cerebellum / Q000187:drug effects / Q000502:physiology*; D005260:Female; D008297:Male; D008464:Mecamylamine / Q000494:pharmacology*; D015999:Multivariate Analysis; D009760:Nystagmus, Physiologic / Q000187:drug effects*; D010830:Physostigmine / Q000494:pharmacology*; D011817:Rabbits; D012016:Reference Values; D012027:Reflex, Vestibulo-Ocular / Q000187:drug effects*; D015348:Vision, Binocular; D015349:Vision, Monocular
chemical_list: D008464:Mecamylamine; D001285:Atropine; D002217:Carbachol; D010830:Physostigmine