Effect of modifications of semen diluent with cell culture serum replacements on fresh and stored turkey semen quality and hen fertility.
Two commercially available serum replacements formulated as supplements to somatic cell culture media were used with a turkey semen diluent to determine their effect on semen quality and spermatozoal fecundity before and after semen storage for 24 h. Progressive motility estimates (visual) and spermatozoal motility characteristics estimated by a computer-assisted semen analysis system, and sperm viability tests (the ethidium bromide exclusion procedure and a sperm stress test) were used to evaluate the semen. Although the presence of the serum replacements significantly increased spermatozoal motility, fertility was not augmented by the presence of the serum replacements. Computer-derived spermatozoal velocity and the percentage of nonviable spermatozoa estimated by the sperm stress test were negatively correlated with hen fertility. It was concluded that although the serum replacements augmented some spermatozoal motility characteristics, they failed to improve the fecundity of spermatozoa either before or after semen storage for 24 h.
Bakst
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U.S. Department of Agriculture, Germplasm and Gamete Physiology Laboratory, Beltsville, Maryland 20705-2350.
Cecil
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D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival; D005260:Female; D005298:Fertility; D007315:Insemination, Artificial / Q000662:veterinary*; D008297:Male; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation / Q000662:veterinary*; D013081:Sperm Motility; D014422:Turkeys / Q000502:physiology*