title: Chemical modification of bovine pericardium and its effect on calcification in the rat subdermal model.
abstract: Specific modification of functional groups in collagen has been used to investigate their influence on calcification and thermal stability of bovine pericardium. Pretreatment of pericardium with iron (III) citrate reduced calcification in the rat subcutaneous implant model, as did acyl azide activation of carboxyl and amide groups. Chondroitin sulphate had no significant effect, while cyanamide treatment was mainly effective in combination with iron (III) citrate. Glutaraldehyde pretreatment restricted reaction with other modifying agents, but, as a post-fixation treatment, improved the thermal stability of other agents. Glutaraldehyde post-fixation had no significant relationship to the calcification rate.
authors.affiliation: Department of Cardiac Surgery, Royal Infirmary, Glasgow, UK.
authors.forename: G M
authors.initials: GM
authors.lastname: Bernacca
authors.forename: W R
authors.initials: WR
authors.lastname: Dimitri
authors.forename: A C
authors.initials: AC
authors.lastname: Fisher
authors.forename: T G
authors.initials: TG
authors.lastname: Mackay
authors.forename: D J
authors.initials: DJ
authors.lastname: Wheatley
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001705:Bioprosthesis; D002114:Calcinosis / Q000209:etiology / Q000517:prevention & control*; D002417:Cattle; D003094:Collagen / Q000737:chemistry; D003432:Cross-Linking Reagents; D005290:Ferric Compounds; D005404:Fixatives; D005976:Glutaral; D006350:Heart Valve Prosthesis / Q000009:adverse effects*; D008297:Male; D008422:Materials Testing; D010496:Pericardium / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D005290:Ferric Compounds; D005404:Fixatives; C025314:ferric citrate; D003094:Collagen; D005976:Glutaral