Detection of oligoclonal IgG bands in cerebrospinal fluid by immunofixation after isoelectric focusing on polyacrylamide gels with the PhastSystem.
This paper describes the detection of oligoclonal bands of IgG immunoglobulins in cerebrospinal fluids (CSF) using isoelectric focusing (IEF) and immunofixation with the PhastSystem equipment. The proteins were separated by IEF on polyacrylamide gels, pH 3-9. The IgG immunoglobulins were then fixed by overlaying the gel with antihuman IgG antiserum. Fixed immunoglobulins were detected with silver staining in the PhastSystem Development Unit. One microliter of serum or CSF containing 15 mg/L of IgG was found to be optimal. The procedure had good reproducibility both with regard to IEF and silver staining of the fixed immunoglobulins. The manpower need was reduced, and the procedure has been found to be useful in the clinical laboratory.
Fredman
P
P
Department of Psychiatry and Neurochemistry, University of Göteborg, Mölndal Hospital, Sweden.
D000180:Acrylic Resins; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000418:Albumins / Q000134:cerebrospinal fluid; D002648:Child; D002675:Child, Preschool; D005260:Female; D005404:Fixatives; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D007158:Immunologic Techniques / Q000706:statistics & numerical data; D007223:Infant; D007231:Infant, Newborn; D007525:Isoelectric Focusing / Q000379:methods* / Q000706:statistics & numerical data; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000032:analysis; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000418:Albumins; D005404:Fixatives; D007074:Immunoglobulin G; D012709:Serum Albumin; D000099166:Polyacrylamides