Heterochromatin characterization of sex chromosomes in Triturus marmoratus (Urodela, Salamandridae).
The sex chromosomes of the Iberian marbled newt, Triturus marmoratus, were studied using various banding techniques, including restriction enzyme/nick translation (RE/NT) procedures. Four types of heterochromatin on the sex chromosomes could be distinguished: (1) distamycin A/DAPI and chromomycin A3/distamycin A positive, EcoRI/NT negative, and HaeIII/NT and HinfI/NT positive; (2) distamycin A/DAPI and chromomycin A3/distamycin A positive, but RE/NT negative; (3) AT rich, but RE/NT negative; and (4) distamycin A/DAPI and chromomycin A3/distamycin A positive, EcoRI/NT and HinfI/NT negative, but HaeIII/NT positive. These data suggest a common origin for the terminal heterochromatic domains of both the X and Y chromosomes in this species.
Herrero
P
P
Unidad de Fisiología Animal, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
de la Torre
J
J
Gosálvez
J
J
Sumner
A T
AT
D000818:Animals; D014128:Chromomycin A3; D002871:Chromosome Banding; D004214:Distamycins; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004996:Ethidium / Q000378:metabolism; D005260:Female; D006570:Heterochromatin / Q000737:chemistry*; D008297:Male; D014319:Triturus / Q000235:genetics*; D014960:X Chromosome / Q000737:chemistry*; D014998:Y Chromosome / Q000737:chemistry*
D004214:Distamycins; D006570:Heterochromatin; C030000:stallimycin; D014128:Chromomycin A3; D004706:Endodeoxyribonucleases; D004996:Ethidium