title: [Determination of m-nifedipine and its pharmacokinetic study in rabbits by high-pressure liquid chromatography].
abstract: A high-pressure liquid chromatographic method was developed for determination of m-nifedipine in plasma using a chemical bonded C-18 phase column (YWG-C18 10 microns, made in China) with nitrendipine as internal standard. To increase life of the YWG-C18 column a mixture of methanol and 5 mmol.L-1 phosphate buffer (70:30 vol/vol) was selected as mobile phase with a flow rate of 0.8 ml.min-1. The method was sensitive to m-nifedipine 3 ng.ml-1 plasma and the standard curve was linear from 10 to 1000 ng.ml-1 with correlation coefficient of 0.99. The within-day and day-to-day precisions (CV) of this method were 4.5% and 7.0%, respectively, with recoveries of 95-102% (10-1000 ng.ml-1). There was no interference with nifedipine, amiodarone, propranol, and verapamil. A pharmacokinetic study on m-nifedipine was carried out in 8 rabbits. A better computer fitted to a two-compartment model was observed using 3P87 program. The parameters obtained were as follow: Vc 6.3 L.kg-1, Cl 0.021 L.kg-1.min-1, T1/2 alpha 30 min, T1/2 beta 230 min, AUC 102 micrograms.min.ml-1.
authors.affiliation: Institute of Clinical Pharmacology, Xi'an Medical University, China.
authors.forename: Y B
authors.initials: YB
authors.lastname: Liang
authors.forename: X Y
authors.initials: XY
authors.lastname: Ma
authors.forename: G J
authors.initials: GJ
authors.lastname: Wu
authors.forename: L F
authors.initials: LF
authors.lastname: Wang
authors.forename: D K
authors.initials: DK
authors.lastname: Liu
authors.forename: J F
authors.initials: JF
authors.lastname: Xing
authors.forename: G S
authors.initials: GS
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008297:Male; D009543:Nifedipine / Q000097:blood / Q000493:pharmacokinetics*; D011817:Rabbits
chemical_list: D009543:Nifedipine