Effects of androgen on phosphorylation of chromosomal proteins in mouse submandibular gland.
The effect of androgen on intranuclear distribution of androgen receptor binding sites was studied. The level of the androgen receptor complex in dinucleosomes increased 1 h after androgen treatment and those in the mono- and trinucleosomes increased 3 h after androgen treatment. Investigation by 32P incorporation into the nuclei and active chromatin showed that androgen resulted in an increase in the rate of phosphorylation at 3 h and reduced to the control level at 6 h. These data suggest that association of androgen receptor with the acceptor sites and activities of protein kinase in active chromatin are simultaneously stimulated for activating the transcription by androgen.
Sato
N
N
Department of Biochemistry, Iwate Medical University, School of Dentistry, Morioka, Japan.
Yoshida
M
M
Ota
M
M
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007700:Kinetics; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011944:Receptors, Androgen / Q000187:drug effects / Q000378:metabolism; D013363:Submandibular Gland / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*
D002843:Chromatin; D009687:Nuclear Proteins; D011944:Receptors, Androgen; D013739:Testosterone; D011494:Protein Kinases