title: Two-step tumour targetting in ovarian cancer patients using biotinylated monoclonal antibodies and radioactive streptavidin.
abstract: A new method for intraperitoneal tumour targetting in ovarian cancer using biotinylated monoclonal antibodies (MoAb) and radioactive streptavidin is described. Fifteen patients with histologically documented ovarian carcinoma were injected intraperitoneally with 2 mg of biotinylated MoAb MOv18, followed 3-5 days later by 100-150 micrograms of indium-111 streptavidin, at the specific activity of 280-370 MBq/mg in 500 ml of normal saline. No toxicity was observed. Tumours were imaged from 2 to 48 h after radioactivity injection by recording both planar and single photon emission tomography (SPET) data. All patients underwent surgery 1-8 days later (mean 3 days) after scanning. The resected tumour and normal tissue radioactivity were measured. On the day of surgery, the tumour to normal tissue ratio was 9:1 (range 3:1-30:1) and 45:1 (range 12:1-120:1) for intra- and extraperitoneal samples, respectively. The mean tumor to blood ratio was 14:1 (range 4:1-30:1). The injected dose (i.d.) per gram of tumour was 0.112 (range 0.01-0.3) for recurrences and 0.05 for primary tumour (range 0.005-0.2). Over 24-48 h 14% i.d. (range 8-18% i.d.) was found in the urine, 14% i.d. (range 6-29% i.d.) in the blood and 63% i.d. (range 56-70% i.d.) was still in the peritoneal cavity. These preliminary clinical data suggest that this two-step strategy may be superior to the conventional approach (radiolabelled antibodies) for intraperitoneal radioimmunolocalization and radioimmunotherapy of ovarian cancer.
authors.affiliation: Department of Nuclear Medicine, Istituto Scientifico H. San Raffaele, Sorin Biomedica, Università di Milano, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Paganelli
authors.forename: C
authors.initials: C
authors.lastname: Belloni
authors.forename: P
authors.initials: P
authors.lastname: Magnani
authors.forename: F
authors.initials: F
authors.lastname: Zito
authors.forename: A
authors.initials: A
authors.lastname: Pasini
authors.forename: I
authors.initials: I
authors.lastname: Sassi
authors.forename: M
authors.initials: M
authors.lastname: Meroni
authors.forename: M
authors.initials: M
authors.lastname: Mariani
authors.forename: M
authors.initials: M
authors.lastname: Vignali
authors.forename: A G
authors.initials: AG
authors.lastname: Siccardi
mesh_terms: D001426:Bacterial Proteins / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007205:Indium Radioisotopes / Q000627:therapeutic use*; D010051:Ovarian Neoplasms / Q000000981:diagnostic imaging* / Q000532:radiotherapy*; D016719:Radioimmunodetection; D016499:Radioimmunotherapy; D019809:Streptavidin
chemical_list: D001426:Bacterial Proteins; D007205:Indium Radioisotopes; D019809:Streptavidin