Reductive dehalogenation of chlorophenols by Desulfomonile tiedjei DCB-1.
Reductive dehalogenation of chlorophenols has been reported in undefined anaerobic cultures but never before in an anaerobic pure culture. We found that the sulfate-reducing bacterium Desulfomonile tiedjei DCB-1 reductively dehalogenates pentachlorophenol (PCP) and other chlorophenols. The maximum rate of PCP dechlorination observed was 54 mu mol of Cl- h-1 g of protein-1. 3-Chlorobenzoate appeared to serve as a required inducer for PCP dehalogenation; however, neither PCP nor 3-chlorophenol induced dehalogenation. Dehalogenation was catalyzed by living cells, and formate served as a required electron donor. D. tiedjei dehalogenated meta-chlorine substituents of chlorophenols (i.e., PCP was degraded to 2,4,6-trichlorophenol). Generally, more highly chlorinated phenol congeners were more readily dechlorinated, and 3-chlorophenol was not dehalogenated. Growing cultures dehalogenated PCP, but greater than 10 microM PCP (approximately 1.7 mmol g of protein-1) reversibly inhibited growth.
Mohn
W W
WW
Division of Biological Sciences, National Research Council Canada, Ottawa, Ontario.
Kennedy
K J
KJ
D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002713:Chlorine / Q000378:metabolism; D002733:Chlorophenols / Q000378:metabolism*; D010084:Oxidation-Reduction
D002733:Chlorophenols; D002713:Chlorine