title: Identification of a nodD-like gene in Frankia by direct complementation of a Rhizobium nodD-mutant.
abstract: Clones from a Frankia At4 gene bank were pooled into groups and mass conjugated into a nodD mutant of Rhizobium leguminosarum bv. viciae by triparental matings. When peas were inoculated with the pooled transconjugants, nodulation was observed. A plasmid, pAt2GX containing Frankia DNA, was isolated from bacteria recovered from these nodules. This plasmid was shown to complement a nodD mutant of R. leguminosarum bv. viciae. Thus pAt2GX contains a Frankia gene that is functionally equivalent to nodD of R. leguminosarum bv. viciae.
authors.affiliation: Laboratory of Molecular Genetics, Guangxi Agricultural College, Nanning, China.
authors.forename: L M
authors.initials: LM
authors.lastname: Chen
authors.forename: Y H
authors.initials: YH
authors.lastname: Cui
authors.forename: M
authors.initials: M
authors.lastname: Qin
authors.forename: Y L
authors.initials: YL
authors.lastname: Wang
authors.forename: X L
authors.initials: XL
authors.lastname: Bai
authors.forename: Q S
authors.initials: QS
authors.lastname: Ma
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D009154:Mutation; D009235:Myxomycetes / Q000235:genetics*; D010957:Plasmids; D016961:Rhizobium leguminosarum / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C060773:NodD protein, Bacteria