title: Human immunodeficiency virus infection in microglia: correlation between cells infected in the brain and cells cultured from infectious brain tissue.
abstract: In acquired immunodeficiency syndrome, the lesions of the central nervous system in association with the human immunodeficiency virus are thought to be related to an infection of microglia, although no studies are available in which cultured and physiological characteristics of microglia cells infected in vivo have been examined. In this report, we used brain tissue from a child dying of human immunodeficiency virus infection and show that microglia cells were the main cell population being infected. Moreover, isolated macrophage-like cells from fresh brain material revealed a close resemblance to peripheral blood macrophages in their content of surface and intracellular antigens. No virus particles or viral antigens were produced by these cells during the first week of cultivation. Productive infection was readily apparent, however, by day 30. This finding illustrates the slow nature of the virus life cycle in these cells and the minimal cytopathology that accompanied the infection.
authors.affiliation: Institut für Virologie und Immunobiologie, Johns Hopkins University School of Medicine, Baltimore, MD.
authors.forename: R
authors.initials: R
authors.lastname: Brinkmann
authors.forename: A
authors.initials: A
authors.lastname: Schwinn
authors.forename: O
authors.initials: O
authors.lastname: Narayan
authors.forename: C
authors.initials: C
authors.lastname: Zink
authors.forename: H W
authors.initials: HW
authors.lastname: Kreth
authors.forename: W
authors.initials: W
authors.lastname: Roggendorf
authors.forename: R
authors.initials: R
authors.lastname: Dörries
authors.forename: S
authors.initials: S
authors.lastname: Schwender
authors.forename: H
authors.initials: H
authors.lastname: Imrich
authors.forename: V
authors.initials: V
authors.lastname: ter Meulen
mesh_terms: D001921:Brain / Q000382:microbiology / Q000473:pathology*; D002478:Cells, Cultured; D002675:Child, Preschool; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D006678:HIV / Q000302:isolation & purification; D015658:HIV Infections / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D009457:Neuroglia / Q000473:pathology*; D009693:Nucleic Acid Hybridization
chemical_list: D005404:Fixatives; D005557:Formaldehyde