A phylogenetic analysis based on the gene encoding phosphoglycerate kinase.
We have determined the nucleotide sequence of both genomic and complementary DNA (cDNA) for the gene encoding the glycolytic enzyme phosphoglycerate kinase from the ciliated protozoan Tetrahymena thermophila. The amino acid sequence for the enzyme has also been derived from the cDNA sequence. The gene contains an open reading frame of 1260 nucleotides encoding 420 amino acids. Coding sequence in genomic DNA is interrupted by two introns at positions corresponding to introns 3 and 4 in mammalian phosphoglycerate kinase genes. The derived amino acid sequence was used to prepare a phylogeny by aligning the Tetrahymena sequence with 25 other phosphoglycerate kinase amino acid sequences. The Tetrahymena sequence is a typical eukaryotic sequence. There is recognizable and clear homology across species that cover nearly the complete range of life forms. The phylogenetic reconstruction of these sequences generally supports the conclusions that have been reached using rRNA sequences.
Vohra
G B
GB
Department of Biology, York University, Toronto, Ontario, Canada.
Golding
G B
GB
Tsao
N
N
Pearlman
R E
RE
D000595:Amino Acid Sequence; D000818:Animals; D001105:Archaea / Q000235:genetics; D001419:Bacteria / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000235:genetics; D056890:Eukaryota / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005658:Fungi / Q000235:genetics; D005796:Genes; D007527:Isoenzymes / Q000235:genetics; D008322:Mammals / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D010944:Plants / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D016808:Tetrahymena thermophila / Q000235:genetics
D001426:Bacterial Proteins; D003062:Codon; D005656:Fungal Proteins; D007527:Isoenzymes; D010940:Plant Proteins; D015800:Protozoan Proteins; D004247:DNA; D010735:Phosphoglycerate Kinase