[Study of humoral hemolytic factors in hemorrhage using chromatographic methods].
The kidney-dependent increase of the haemolytic activity of blood serum after acute decompensated blood loss was demonstrated in the experiments on rats. The preparative ion-exchange chromatography on DEAE-Toyoperal was used to separate the haemolytic active component of the posthaemorrhagic blood serum with the max value of the specific activity of 6.86 A/micrograms protein. The analysis of the separated component by size-exclusion chromatography on TSK-G3000-SW column indicated a molecular mass of 80-100 kDa. In injection in the circulatory system in in vivo experiments the dose-dependent effect of the action of separated component was demonstrated.
Ershova
L I
LI
Zeĭnalov
A M
AM
Azhigirova
M A
MA
Dereza
T L
TL
Kurbanova
G N
GN
Gorbunova
N A
NA
D000818:Animals; D002852:Chromatography, Ion Exchange; D006461:Hemolysis; D006470:Hemorrhage / Q000097:blood*; D007668:Kidney / Q000502:physiology*; D008954:Models, Biological; D009392:Nephrectomy; D051381:Rats; D011919:Rats, Inbred Strains
Issledovanie gumoral'nykh gemoliticheskikh faktorov pri krovopotere khromatograficheskimi metodami.