title: Calcium-induced acetylcholine release and intramembrane particle occurrence in proteoliposomes equipped with mediatophore.
abstract: Proteoliposomes obtained from the mediatophore, a purified Torpedo electric organ nerve terminals protein, and endogenous lipids were used for a study of calcium-induced release of acetylcholine and freeze-fracture electron microscopy. Large intramembrane particles were induced by the influx of calcium into proteoliposomes, as previously observed for synaptosomes or stimulated electric organ nerve terminals. The involvement of mediatophore in a calcium dependent acetylcholine translocation seems therefore to be related to the occurrence of a category of intramembrane particles in the course of the release process.
authors.affiliation: Département de Neurochimie, CNRS, Gif-sur-Yvette, France.
authors.forename: G
authors.initials: G
authors.lastname: Brochier
authors.forename: T
authors.initials: T
authors.lastname: Gulik-Krzywicki
authors.forename: B
authors.initials: B
authors.lastname: Lesbats
authors.forename: J C
authors.initials: JC
authors.lastname: Dedieu
authors.forename: M
authors.initials: M
authors.lastname: Israël
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005614:Freeze Fracturing; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011510:Proteolipids / Q000378:metabolism* / Q000648:ultrastructure; D014101:Torpedo
chemical_list: D008081:Liposomes; D009419:Nerve Tissue Proteins; D011510:Proteolipids; C050299:mediatophore; C020485:proteoliposomes; D000109:Acetylcholine; D002118:Calcium