Human dopa decarboxylase: localization to human chromosome 7p11 and characterization of hepatic cDNAs.
We have cloned full-length DDC cDNAs from a human hepatoma cDNA library [DDC; dopa decarboxylase; aromatic-L-amino acid decarboxylase, EC 4.1.1.28]. The protein encoded by hepatoma cells is the same as that encoded by adrenal chromaffin derived pheochromocytoma cells, despite reported differences in biochemical properties. We have confirmed the location of the DDC gene to chromosome 7 using a new panel of somatic cell hybrids, and we have localized the gene to band p11 on chromosome 7 by fluorescent in situ hybridization. The human gene retains 65% amino acid identity with Drosophila DDC (Accession No. X04426) and considerable structural similarity with other enzymes (F.R. Jackson, 1990, J. Mol. Evol. 31:325-329, and references therein).
Scherer
L J
LJ
Developmental Biology Center, University of California, Irvine 92717.
McPherson
J D
JD
Wasmuth
J J
JJ
Marsh
J L
JL
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D004296:Dopa Decarboxylase / Q000235:genetics*; D005456:Fluorescent Dyes; D005796:Genes; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
D005456:Fluorescent Dyes; D004247:DNA; D004296:Dopa Decarboxylase