title: Separation of hematopoietic stem cells into two populations and their characterization.
abstract: Two populations of hematopoietic stem cells (HSC) in mouse bone marrow (BM) are defined on the basis of the presence or absence of interleukin-3 (IL-3) receptor-associated antigen (IL-3RAA). HSC were purified by depletion of mature lymphoid-lineage cells followed by collection of the low-density fraction and sorting of wheat germ agglutinin-binding (WGA+) cells using a fluorescein-activated cell sorter. WGA+ cells were further separated into two populations (IL-3RAA+/WGA+ and IL-3RAA-/WGA+) by a monoclonal antibody (MoAb) against IL-3RAA. IL-3RAA+/WGA+ cells formed CFU-S on day 8; this population consisted mainly of cells in the cycling phase. IL-3RAA-/WGA+ cells form CFU-S on day 12; this population consisted mainly of dormant cells (cells in the G0 phase). When two populations obtained from C3H/HeN mice were injected into lethally irradiated (C57BL/6 x C3H/HeN)F1 mice, donor-derived cells in the peripheral blood (PB) appeared significantly earlier in mice injected with IL-3RAA+/WGA+ cells than in those injected with IL-3RAA-/WGA+ cells, whereas the reconstruction efficiency of IL-3RAA-/WGA+ cells had overtaken that of IL-3RAA+/WGA+ cells 6 weeks after injection. Long-term observation showed no significant difference between these two populations, however. The radioprotective ability (RPA) (30-day survival) of these two populations was therefore compared. The RPA of IL-3RAA-/WGA+ cells was significantly higher than that of IL-3RAA+/WGA+ cells. These findings therefore suggest that the former population is more primitive.
authors.affiliation: First Department of Pathology, Kansai Medical University, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ogata
authors.forename: S
authors.initials: S
authors.lastname: Taniguchi
authors.forename: M
authors.initials: M
authors.lastname: Inaba
authors.forename: M
authors.initials: M
authors.lastname: Sugawara
authors.forename: Y
authors.initials: Y
authors.lastname: Ohta
authors.forename: K
authors.initials: K
authors.lastname: Inaba
authors.forename: K J
authors.initials: KJ
authors.lastname: Mori
authors.forename: S
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D016026:Bone Marrow Transplantation; D002453:Cell Cycle / Q000187:drug effects; D002469:Cell Separation; D005434:Flow Cytometry; D006410:Hematopoiesis / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007377:Interleukin-3 / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D011828:Radiation Chimera; D016185:Receptors, Interleukin-3 / Q000276:immunology / Q000378:metabolism*; D014909:Wheat Germ Agglutinins / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D007377:Interleukin-3; D016185:Receptors, Interleukin-3; D014909:Wheat Germ Agglutinins