title: Water and electrolyte contents, cell pH, and membrane potential of primary cultures of astrocytes from DBA, C57, and SW mice.
abstract: Some basic properties of primary cultures of astrocytes derived from the cerebral cortex of an audiogenic seizure-sensitive strain of mice, DBA/2J (DBA), were studied with different approaches. The results were compared with those of audiogenic seizure-resistant strains, C57BL/6J (C57) and Swiss Webster (SW). Contents of intracellular water, protein, and DNA of DBA astrocytes were 0.673 +/- 0.019 ml/g cells, 0.082 +/- 0.006 g/g cells, and 0.0072 +/- 0.0005 g/g cells, respectively. These results are not different from those of either C57 or SW astrocytes. Intracellular concentration of K+, Na+, and Cl- ([K+]1, [Na+]1, and [Cl-]1) derived from the flame photometric and from the radioisotope uptake data of DBA astrocytes were 120.4 +/- 8.5, 25.9 +/- 3.2, and 26.8 +/- 1.8 mM/L cell H2O, respectively. [Na+]1 and [Cl-]1 in DBA astrocytes were lower than those in C57 and SW astrocytes. In DBA astrocytes, SITS decreased the cell/medium ratio (C/M) of 36Cl- and increased the C/M of 125I-; ouabain increased the C/M of 22Na+ and decreased the C/M of 125I-; bumetanide decreased the C/M of both 36Cl- and 22Na+; and NaClO4 decreased the C/M of 125I-. Similar results were observed in both C57 and SW astrocytes. Intracellular pH (pHi) as determined with 14C-DMO of astrocytes in HEPES-buffered saline solution averaged 7.04 +/- 0.03 for DBA, 7.01 +/- 0.02 for C57, and 6.97 +/- 0.02 for SW mice when pH of medium was maintained at 7.4. Modification of ion (HCO3-, Cl-, Na+, and K+) concentration and pH of culture medium all changed the pHi of astrocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology, University of Utah School of Medicine, Salt Lake City.
authors.forename: S Y
authors.initials: SY
authors.lastname: Chow
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: D M
authors.initials: DM
authors.lastname: Woodbury
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D002713:Chlorine / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007457:Iodine Radioisotopes / Q000378:metabolism; D008564:Membrane Potentials; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism*; D011868:Radioisotopes / Q000378:metabolism; D012640:Seizures / Q000235:genetics / Q000378:metabolism; D012979:Sodium Radioisotopes / Q000378:metabolism; D013045:Species Specificity; D014882:Water-Electrolyte Balance
chemical_list: D007457:Iodine Radioisotopes; D011868:Radioisotopes; D012979:Sodium Radioisotopes; D002713:Chlorine; D004247:DNA