Synergistic interaction of cyclosporin A and verapamil on vincristine and daunorubicin resistance in multidrug-resistant human leukemia cells in vitro.
We studied the effects of cyclosporin A and verapamil on the modulation of vincristine and daunorubicin resistance in a multidrug-resistant subline of human T-cell acute lymphatic leukemia GM3639. Our results show that cyclosporin A is more effective than verapamil as a modulator of the high degree of primary vincristine resistance and the low degree of daunorubicin cross-resistance expressed by this cell line. Isobologram analysis revealed that the combined modulators act synergistically in correcting both vincristine and daunorubicin resistance.
Osann
K
K
Department of Medicine, College of Medicine, University of California, Irvine 92717.
Sweet
P
P
Slater
L M
LM
D016572:Cyclosporine / Q000494:pharmacology*; D003630:Daunorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D004357:Drug Synergism; D006801:Humans; D007700:Kinetics; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014700:Verapamil / Q000494:pharmacology*; D014750:Vincristine / Q000494:pharmacology*
D014750:Vincristine; D016572:Cyclosporine; D014700:Verapamil; D003630:Daunorubicin