Effect of glycosaminoglycans on peptide bond formation in bacterial ribosomes.
1. A cell-free system derived from E. coli has been used in this study. The process of peptide bond formation was assessed with the aid of the puromycin reaction, which is catalyzed by peptidyltransferase. 2. This reaction is inhibited by heparin, in contrast, this reaction is activated by hyaluronic acid. 3. The presence of heparin decreases the percentage of formed initiation complex (complex C), but hyaluronic acid, chondroitin sulphate and keratan sulphate have no effect on the formation of complex C. 4. From other types of glycosaminoglycans, only hyaluronic acid increases the stability of active complex C.
Theocharis
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Laboratory of Biological Chemistry, School of Health Sciences, University of Patras, Greece.
D002809:Chondroitin Sulfates / Q000502:physiology; D004926:Escherichia coli / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000502:physiology*; D006493:Heparin / Q000502:physiology; D006820:Hyaluronic Acid / Q000502:physiology; D007632:Keratan Sulfate / Q000502:physiology; D007700:Kinetics; D010455:Peptides / Q000378:metabolism*; D011691:Puromycin / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D006025:Glycosaminoglycans; D010455:Peptides; D012346:RNA, Transfer, Amino Acyl; C006383:tRNA, N-acetylphenylalanine-; D011691:Puromycin; D006820:Hyaluronic Acid; D006493:Heparin; D002809:Chondroitin Sulfates; D007632:Keratan Sulfate