Capacity of listeriolysin O, streptolysin O, and perfringolysin O to mediate growth of Bacillus subtilis within mammalian cells.
The Listeria monocytogenes hemolysin listeriolysin O (LLO) plays a major role in mediating the escape of L. monocytogenes from a vacuolar compartment. In a previous report, it was shown that Bacillus subtilis expressing LLO could escape from a host vacuolar compartment and grow in the cytoplasm (J. Bielecki, P. Youngman, P. Connelly, and D. A. Portnoy, Nature [London] 345:175-176, 1990). In the present study, two related thiol-activated hemolysins, streptolysin O (SLO) and perfringolysin O (PFO), were expressed in B. subtilis and their ability to mediate intracellular growth was monitored by visual inspection and by assaying for CFU. Like LLO, PFO was active within the vacuolar environment, whereas SLO showed negligible activity. However, expression of PFO seemed to damage the host cells. The pH of the vacuole probably had little to do with these results, since all three hemolysins showed full or enhanced activity at pH 5.5, although LLO showed greatly reduced activity at pH 7. In addition, neutralization of the pH within host vacuoles by using weak bases had little effect on the lysis of the vacuole. The lack of SLO activity is probably caused by its lower specific activity; the purified protein had 10-fold less activity on a molar basis. These results suggest that LLO is not unique in its capacity to mediate intracellular growth of B. subtilis.
Portnoy
D A
DA
Department of Microbiology, University of Pennsylvania, School of Medicine, Philadelphia 19104-6076.
Tweten
R K
RK
Kehoe
M
M
Bielecki
J
J
D000818:Animals; D001412:Bacillus subtilis / Q000472:pathogenicity*; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006360:Heat-Shock Proteins / Q000502:physiology*; D006460:Hemolysin Proteins / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D010957:Plasmids; D013301:Streptolysins / Q000502:physiology*; D013997:Time Factors; D014161:Transduction, Genetic; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D013301:Streptolysins; C025964:streptolysin O; C020452:Clostridium perfringens theta-toxin; C038705:hlyA protein, Listeria monocytogenes