Development of mouse enucleated oocytes receiving a nucleus from different stages of the second cell cycle.
The influence of the stage of the cell cycle of donor nuclei on the development of mouse oocytes enucleated at telophase I was examined. After nuclear transplantation and activation, a high proportion of the oocytes remodelled a nucleus, emitted a polar body and formed a pronuclear-like nucleus. Most of the reconstituted embryos that received an interphase nucleus 30-32 h or 34-36 h after treatment with human chorionic gonadotrophin (hCG) arrested at the 2-cell stage. The reconstituted embryos were able to develop to blastocysts when nuclei from late 2-cell embryos (44-46 and 48-50 h after hCG) were transferred to the oocytes. The resulting blastocysts were transferred to recipients and ten live young were obtained from the embryos that formed a pronuclear-like nucleus after extrusion of a polar body. Thus, the developmental ability of the reconstituted embryos was critically influenced by the stage of the cell cycle of the donor nuclei.
Kono
T
T
NODAI Research Institute, Tokyo, Japan.
Kwon
O Y
OY
Watanabe
T
T
Nakahara
T
T
D000818:Animals; D001757:Blastomeres / Q000166:cytology* / Q000187:drug effects; D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D002875:Chromosomes; D005314:Embryonic and Fetal Development / Q000187:drug effects / Q000502:physiology*; D007399:Interphase; D051379:Mice; D008815:Mice, Inbred Strains; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000502:physiology*; D013692:Telophase
D006063:Chorionic Gonadotropin