title: The possible role of ADP ribosylation in physiological regulation of sporulation in Streptomyces griseus.
abstract: The role of ADP ribosylation of proteins in the physiological regulation of sporulation in Streptomyces griseus was studied. We report here that both the activity of NAD+: arginine ADP-ribosyltransferase (ADPRT) and the pattern of ADP-ribosylated proteins showed characteristic changes during the life cycle in S. griseus 2682. Analysis off ADP-ribosylated proteins revealed that in a nonsporulating mutant of the parental wild-type (wt) strain (Bld7 mutant), both the activity of ADPRT and the pattern of ADP-ribosylated proteins were different from those of the parental strain. Addition of 3-aminobenzamide (3AB), the most potent inhibitor of ADPRT, inhibited sporulation of S. griseus 2682 and the A-factor (AF)-induced sporulation of S. griseus Bld7, but in both cases the inhibitory effect of 3AB was strictly age-dependent. Using [alpha-32P]GTP, we have demonstrated the presence of GTP-binding proteins in purified cell membranes of S. griseus 2682 and S. griseus Bld7. The same GTP-binding proteins were observed in Bld7 and the wt. AF stimulated the basal GTPase activity of cell membranes of S. griseus 2682 in a concentration-dependent manner, suggesting that GTP-binding proteins might be involved in the AF-induced sporulation process.
authors.affiliation: Department of Biology, University Medical School, Debrecen, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Penyige
authors.forename: G
authors.initials: G
authors.lastname: Vargha
authors.forename: J C
authors.initials: JC
authors.lastname: Ensign
authors.forename: G
authors.initials: G
authors.lastname: Barabás
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D001549:Benzamides / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism; D007700:Kinetics; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology; D013305:Streptomyces griseus / Q000201:enzymology / Q000235:genetics / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D000246:Adenosine Diphosphate Ribose; D000244:Adenosine Diphosphate; C025160:3-aminobenzamide; D011065:Poly(ADP-ribose) Polymerases; D019204:GTP-Binding Proteins