title: Screening method for beta-lactamase substrate profiles.
abstract: A disc diffusion method, based on the idea of Klundert, for screening of substrate profiles of beta-lactamases was developed in order to perform epidemiological studies. The method was tested against 30 different reference beta-lactamases and 59 clinical isolates known to produce TEM-1, SHV-1 and BRO-1. The reproducibility and discriminating power of the disc diffusion method made it possible to differentiate between eight types of substrate profiles for the 30 reference beta-lactamases and to differentiate between TEM-1, SHV-1 and BRO-1 from clinical isolates. In combination with analytical isoelectric focusing the disc diffusion method gives a reliable identification of beta-lactamases.
authors.affiliation: Department of Hospital Infections and Antibiotics, Immunological R & D, Copenhagen, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Schumacher
authors.forename: M
authors.initials: M
authors.lastname: Nir
authors.forename: B
authors.initials: B
authors.lastname: Mansa
authors.forename: V F
authors.initials: VF
authors.lastname: Thomsen
mesh_terms: D000900:Anti-Bacterial Agents / Q000592:standards; D001419:Bacteria / Q000201:enzymology*; D004754:Enterobacter / Q000201:enzymology; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000201:enzymology; D006801:Humans; D007525:Isoelectric Focusing; D007709:Klebsiella / Q000201:enzymology; D007710:Klebsiella Infections / Q000188:drug therapy / Q000201:enzymology; D008722:Methods; D011549:Pseudomonas / Q000201:enzymology; D015203:Reproducibility of Results; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000032:analysis* / Q000145:classification; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases