Variations of lectin-binding glycoproteins in early pregnant rabbit embryo.
In the present study, the quantitative and qualitative changes of three kinds of lectin-binding glycoproteins of early pregnant rabbit embryos (D4-D12) were analyzed. The technique of Western blot, as well as video densitometer scanning and its analytic software was used for analysis. Results found that there were five specific lectin-binding glycoproteins in Day 4 to Day 6 blastocyst fluids: one ConA-binding glycoprotein about 70 kD, two WGA-binding glycoproteins respectively about 42 kD and 25 kD and two PNA-binding glycoprotein respectively about 180 kD and 75 kD. They disappeared immediately after implantation. It is demonstrated that there are stage-specific glycoproteins in rabbit blastocyst fluid which might be relevant to the recognition of pregnancy and implantation.
Chen
S Z
SZ
Department of Endocrinology, Institute of Zoology, Academia Sinica, Beijing, PRC.
Cao
Y Q
YQ
D000818:Animals; D001755:Blastocyst / Q000737:chemistry; D003208:Concanavalin A; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism*; D037102:Lectins; D019887:Peanut Agglutinin; D011247:Pregnancy; D011817:Rabbits; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D037102:Lectins; D019887:Peanut Agglutinin; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A