title: Baculovirus-expressed glycoprotein E (gE) of herpes simplex virus type-1 (HSV-1) protects mice against lethal intraperitoneal and lethal ocular HSV-1 challenge.
abstract: We have constructed a recombinant baculovirus expressing high levels of the herpes simplex virus type 1 (HSV-1) glycoprotein E (gE) in Sf9 cells. The expressed gE migrated on gels as a double band with apparent molecular weights of 68 and 70 kDa. The recombinant gE was glycosylated based on its susceptibility to tunicamycin treatment and was transported to the membrane of Sf9 cells based on indirect immunofluorescence. Mice vaccinated with gE developed high serum titers of HSV-1-neutralizing antibodies based on plaque reduction assays. gE vaccination also induced a strong delayed type hypersensitivity (DTH) response to HSV-1. In addition, mice vaccinated with the recombinant gE were protected from both intraperitoneal and ocular lethal HSV-1 challenge. To our knowledge, this is the first report in which vaccination with gE was shown to induce high neutralizing antibody titers, a DTH response, or protection against lethal HSV-1 challenge.
authors.affiliation: Ophthalmology Research, Cedars-Sinai Medical Center, Los Angeles, California 90048.
authors.forename: H
authors.initials: H
authors.lastname: Ghiasi
authors.forename: R
authors.initials: R
authors.lastname: Kaiwar
authors.forename: A B
authors.initials: AB
authors.lastname: Nesburn
authors.forename: S
authors.initials: S
authors.lastname: Slanina
authors.forename: S L
authors.initials: SL
authors.lastname: Wechsler
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D005822:Genetic Vectors; D006031:Glycosylation; D006561:Herpes Simplex / Q000276:immunology / Q000517:prevention & control*; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular; D007274:Injections, Intraperitoneal; D016849:Keratitis, Herpetic / Q000276:immunology / Q000517:prevention & control; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000737:chemistry*
chemical_list: D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C044768:glycoprotein E, herpes simplex virus type 1