title: AMP deaminase histochemical activity and immunofluorescent isozyme localization in rat skeletal muscle.
abstract: The cellular distribution of AMP deaminase (AMPda) isozymes was documented for rat soleus and plantaris muscles, utilizing immunofluorescence microscopy and immunoprecipitation methods. AMPda is a ubiquitous enzyme existing as three distinct isozymes, A, B and C, which were initially purified from skeletal muscle, liver (and kidney), and heart, respectively. AMPda-A is primarily concentrated subsarcolemmally and intermyofibrillarly within muscle cells, while isozymes B and C are concentrated within non-myofiber elements of muscle tissue. AMPda-B is principally associated with connective tissues surrounding neural elements and the muscle spindle capsule, and AMPda-C is predominantly associated with circulatory elements, such as arterial and venous walls, capillary endothelium, and red blood cells. These specific localizations, combined with documented differences in kinetic properties, suggest multiple functional roles for the AMPda isozymes or temporal segregation of similar AMPda functions. Linkage of the AMPda substrate with adenosine production pathways at the AMP level and the localization of isozyme-C in vascular tissue suggest a regulatory role in the microcirculation.
authors.affiliation: Department of Cellular Biology and Anatomy, Medical College of Wisconsin, Milwaukee.
authors.forename: J L
authors.initials: JL
authors.lastname: Thompson
authors.forename: R L
authors.initials: RL
authors.lastname: Sabina
authors.forename: N
authors.initials: N
authors.lastname: Ogasawara
authors.forename: D A
authors.initials: DA
authors.lastname: Riley
mesh_terms: D000659:AMP Deaminase / Q000032:analysis / Q000737:chemistry*; D000818:Animals; D003238:Connective Tissue / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis; D008856:Microscopy, Fluorescence; D009132:Muscles / Q000201:enzymology*; D009210:Myofibrils / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D012508:Sarcolemma / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D000659:AMP Deaminase