title: The physical and mechanical effects of suspension-induced osteopenia on mouse long bones.
abstract: The present investigation addresses the extent of tail-suspension effects on the long bones of mice. The effects are explored in both sexes, in both forelimb and hindlimb bones, and in both diaphyseal and metaphyseal/epiphyseal bones. Two weeks of suspension provided unloading of the femora and tibiae and an altered loading of the humeri. Whole-bone effects included lower mass (approximately 10%) and length (approximately 4%) in the bones of suspended mice compared to controls. The geometric and material properties of the femora were considered along the entire length of the diaphysis and in the metaphysis/epiphysis portions as a unit. Geometric effects included lower cross-sectional cortical area (16%), cortical thickness (25%) and moment of inertia (21%) in the femora of suspended mice; these differences were observed in both distal and proximal portions of the femur diaphysis. The relative amount of bone comprising the middle 8 mm of the diaphysis was greater (3%) in the control mice than in the suspended mice. Significant mass differences between the group in the metaphysis/epiphysis were not observed. Material effects included lower %ash (approximately 2%) in the femora and tibiae as well as in the humeri of suspended mice compared to controls. With respect to the measured physical and material properties, suspension produced similar bone responses in male and female mice. The effects of suspension are manifested largely through geometric rather than through material changes.
authors.affiliation: BioServe Space Technologies, University of Colorado, Boulder 80309-0429.
authors.forename: S J
authors.initials: SJ
authors.lastname: Simske
authors.forename: K M
authors.initials: KM
authors.lastname: Guerra
authors.forename: A R
authors.initials: AR
authors.lastname: Greenberg
authors.forename: M W
authors.initials: MW
authors.lastname: Luttges
mesh_terms: D000818:Animals; D001834:Body Water / Q000737:chemistry; D015519:Bone Density; D001851:Bone Diseases, Metabolic / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D001853:Bone Marrow / Q000473:pathology; D004548:Elasticity; D004838:Epiphyses / Q000737:chemistry / Q000473:pathology* / Q000503:physiopathology*; D005260:Female; D005269:Femur / Q000737:chemistry / Q000473:pathology* / Q000503:physiopathology*; D006811:Humerus / Q000737:chemistry / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051379:Mice; D010521:Periosteum / Q000473:pathology; D012737:Sex Factors; D013314:Stress, Mechanical; D013718:Tensile Strength; D013977:Tibia / Q000737:chemistry / Q000473:pathology / Q000503:physiopathology; D016474:Weight-Bearing / Q000502:physiology*