An amino acid change in the DNA-binding region of Sry, found in Mus musculus domesticus and other species, does not explain C57BL/6J-YDOM sex-reversal.
Sry is the testes determining factor gene located on the Y chromosome. We have performed dideoxy sequencing or restriction enzyme digestion of Sry sequences amplified by PCR from a variety of inbred strains and species of mice. A nonconservative replacement of Thr for Isoleu at position 61 was found in Sry from the Y chromosome of Mus musculus domesticus, Mus spretus, and Mus caroli. We demonstrate that this variation is not sufficient to explain the gradiation in the sex-reversal seen when different domesticus strains are bred to C57BL/6J females.
Graves
P E
PE
Steele Memorial Children's Research Center, Department of Pediatrics, University of Arizona, Tucson 85724.
Erickson
R P
RP
D000818:Animals; D001483:Base Sequence; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics*; D012734:Disorders of Sex Development; D005799:Genes, Dominant / Q000235:genetics; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D009117:Muscidae / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; C497746:Sry protein, mouse; D014157:Transcription Factors