Cloning of the guinea pig 5-lipoxygenase gene and nucleotide sequence of its promoter.
The guinea pig 5-lipoxygenase (5-LO) gene and its promoter were cloned from a guinea pig genomic DNA library. Sequencing analysis of the guinea pig promoter revealed that expression of the 5-LO gene in this rodent is probably governed by cis acting nucleotide sequences quite similar to the human gene. Nucleotide sequences that bind factors like Sp-1, AP-2, NF-kB and c-Ha-ras were identified in the guinea pig 5-LO promoter region.
Chopra
A
A
Department of Microbiology, Faculty of Medicine, University of Sherbrooke, Canada.
Ferreira-Alves
D L
DL
Sirois
P
P
Thirion
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005796:Genes; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014157:Transcription Factors / Q000378:metabolism
D014157:Transcription Factors; D001094:Arachidonate 5-Lipoxygenase