title: A sensitive and specific high performance liquid chromatographic assay for imidazole dipeptides and 3-methylhistidine in human muscle biopsies, serum and urine.
abstract: A sensitive and specific high performance liquid chromatographic method is described for measuring imidazole dipeptides and 3-methylhistidine in human muscle biopsies, serum and urine. Muscle extract, serum or urine was reacted with o-phthaldialdehyde and the derivatives were separated by reversed phase chromatography with column switching and fluorescence detection.
authors.affiliation: Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
authors.forename: K
authors.initials: K
authors.lastname: Teahon
authors.forename: J M
authors.initials: JM
authors.lastname: Rideout
mesh_terms: D000861:Anserine / Q000097:blood / Q000378:metabolism* / Q000652:urine; D001706:Biopsy; D002336:Carnosine / Q000097:blood / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008762:Methylhistidines / Q000097:blood / Q000378:metabolism* / Q000652:urine; D009132:Muscles / Q000737:chemistry* / Q000473:pathology; D013050:Spectrometry, Fluorescence
chemical_list: D008762:Methylhistidines; D002336:Carnosine; D000861:Anserine; C028118:3-methylhistidine