The rad3+ gene of Schizosaccharomyces pombe is involved in multiple checkpoint functions and in DNA repair.
A number of important molecular checkpoints are believed to control the orderly progression of cell cycle events. We have found that the radiation-sensitive Schizosaccharomyces pombe mutant rad3-136 is deficient in two molecular checkpoint functions. Unlike wild-type cells, the mutant cells are unable to arrest in the G2 phase of the cell cycle after DNA damage by gamma-irradiation and are also incapable of maintaining the dependence of mitosis upon the completion of DNA synthesis. An S. pombe genomic clone that complements the UV sensitivity of the rad3-136 mutant completely restores the missing checkpoint functions. The rad3+ gene is also likely to play a role in DNA repair.
Jimenez
G
G
Department of Biology, University of California, San Diego, La Jolla 92093.
Yucel
J
J
Rowley
R
R
Subramani
S
S
D001542:Benomyl / Q000494:pharmacology; D002453:Cell Cycle; D003001:Cloning, Molecular; D011088:DNA Ligases / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D005720:Gamma Rays; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006918:Hydroxyurea / Q000494:pharmacology; D008938:Mitosis; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics*
D011088:DNA Ligases; D001542:Benomyl; D006918:Hydroxyurea