Cloning and modulation by endothelin-1 of rat cardiac K channel.
We have cloned a delayed rectifier type K channel from rat heart (RH1). RH1 was identical to the rat brain K channel BK2 and differed from recently cloned rat cardiac K channel RAK by one amino acid residue. Endothelin receptors(ETRs)-mediated modulation of RH1 current (IRH1) was studied using Xenopus oocyte expression system. Activation of two different subtypes of ETRs by endothelin-1 equally suppressed the amplitude of IRH1. Stimulation of phosphatidylinositol turnover will probably be responsible for the suppression.
Ishii
K
K
Department of Pharmacology, Tohoku University School of Medicine, Sendai, Japan.
Nunoki
K
K
Murakoshi
H
H
Taira
N
N
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D016232:Endothelins / Q000494:pharmacology*; D005071:Evoked Potentials / Q000187:drug effects; D005260:Female; D005822:Genetic Vectors; D006321:Heart / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D010716:Phosphatidylinositols / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000187:drug effects / Q000378:metabolism; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D014982:Xenopus laevis
D016232:Endothelins; D010716:Phosphatidylinositols; D015221:Potassium Channels; D012333:RNA, Messenger; D011994:Recombinant Proteins; D013757:Tetraethylammonium Compounds; D019789:Tetraethylammonium; D004247:DNA