The use of radioactive bacteriophage proteins as X-Y markers for silver stained two-dimensional electrophoresis gels and quantification of the patterns.
We have demonstrated the feasibility of using bacteriophage ghost proteins, tritiated by metabolic labeling, as a set of standard markers for two-dimensional gels in which the proteins are to be detected by silver staining. The results indicate that a 2.5 microgram load of phage proteins yields a reproducible silver pattern of 48 spots. The spots can also be readily identified by radioautography and radiofluorography, establishing their value as a standard constellation of markers. Quantification of these patterns by computerized densitometry indicates a general agreement between detection by silver staining and detection by radiofluorography.
Gersten
D M
DM
Department of Pathology, Georgetown University Medical Center, Washington, DC 20007.
Ramagli
L S
LS
Johnston
D A
DA
Rodriguez
L V
LV
D015415:Biomarkers / Q000737:chemistry; D003851:Deoxyribonucleases; D015180:Electrophoresis, Gel, Two-Dimensional; D007526:Isoelectric Point; D016622:Silver Staining; D013604:T-Phages / Q000737:chemistry*; D014316:Tritium; D014741:Video Recording; D014764:Viral Proteins / Q000032:analysis*
D015415:Biomarkers; D014764:Viral Proteins; D014316:Tritium; D003851:Deoxyribonucleases