Glycosaminoglycan patterns in gingival proteoglycans of rat with age.
Among the potential biochemical indices that are closely associated with craniofacial development are the proteoglycans. Gingival segments from the palate of 4-, 6-, 8-, 12- and 18-week-old rats were incubated for 4 h in medium containing [3H]-glucosamine and [35S]-Na2SO4, and subjected to proteoglycan isolation and glycosaminoglycan analysis. Two distinct proteoglycan fractions differing in the degree of sulphation were obtained by ion-exchange chromatography. The incorporation of both labels in the undersulphated fraction increased with age; there was a pronounced decrease with age in the sulphated proteoglycan fraction. The undersulphated proteoglycans showed an age-dependent decrease in hyaluronic acid, and increase in dermatan sulphate and chondroitin 4- and 6-sulphates. Gel filtration of the sulphated proteoglycan fraction yielded high and low molecular-weight proteoglycans, the glycosaminoglycans of which were particularly rich (61-76%) in dermatan sulphate. Smaller quantities of chondroitin 4- and 6-sulphates, and heparan sulphate were also present. All glycosaminoglycans showed a decrease in content with age. The findings suggest a possible correlation between gingival proteoglycan/glycosaminoglycan patterns and development.
Weinstein
M
M
Research Center, New Jersey Dental School, University of Medicine and Dentistry, Newark 07103-2400.
Liau
Y H
YH
Slomiany
A
A
Slomiany
B L
BL
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D046508:Culture Techniques; D005881:Gingiva / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000032:analysis / Q000378:metabolism*; D008970:Molecular Weight; D011509:Proteoglycans / Q000032:analysis / Q000378:metabolism*; D051381:Rats
D006025:Glycosaminoglycans; D011509:Proteoglycans