A new potent inhibitor of horse liver alcohol dehydrogenase: p-methylbenzyl hydroperoxide.
A product of p-xylene auto-oxidation, p-methylbenzyl hydroperoxide, acts as a very strong reversible inhibitor of the ethanol dehydrogenating activity of horse liver alcohol dehydrogenase. Concentrations of hydroperoxide as low as that of the enzyme active site (about 10(-8) mol.dm-3) in the assay depresses the activity by 50%. Somewhat less potent is benzyl hydroperoxide (derived from toluene) while the (secondary) hydroperoxide derived from ethylbenzene and tert.butyl hydroperoxide and cumyl hydroperoxide do not inhibit HLAD appreciably.
Skurský
L
L
Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czechoslovakia.
Khan
A N
AN
Saleem
M N
MN
al-Tamer
Y Y
YY
D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors*; D000818:Animals; D001593:Benzyl Compounds / Q000494:pharmacology*; D006736:Horses; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010545:Peroxides / Q000494:pharmacology
D001593:Benzyl Compounds; D010545:Peroxides; C075152:4-methylbenzyl hydroperoxide; D000426:Alcohol Dehydrogenase