Cloning and expression in yeast of a plant potassium ion transport system.
A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+ uptake with a complementary DNA library from Arabidopsis thaliana. A 2.65-kilobase complementary DNA conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or 86Rb+) at rates similar to those in wild-type yeast. The predicted amino acid sequence (838 amino acids) has three domains: a channel-forming region homologous to animal K+ channels, a cyclic nucleotide-binding site, and an ankyrin-like region.
Sentenac
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H
Biochimie et Physiologie Végétales, ENSA-M/INRA/CNRS URA 573, Montpellier, France.
Bonneaud
N
N
Minet
M
M
Lacroute
F
F
Salmon
J M
JM
Gaymard
F
F
Grignon
C
C
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001692:Biological Transport; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D015870:Gene Expression; D007700:Kinetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D010940:Plant Proteins; D015221:Potassium Channels; C074500:AKT1 protein, Arabidopsis; D004247:DNA; D015246:Deoxyribonuclease EcoRI; D011188:Potassium