[A modified method of endothelium impregnation suitable for pathology studies].
Prolonged fixation worsens the quality of the endothelium boundaries silver impregnation. A modified method is suggested: the vessel segment is fixed in 2.5% glutaraldehyde for 5 min with subsequent conservation in the saline till the impregnation. Incubation in the saline for 24 hours preserves the susceptibility of endothelium to silver impregnation.
Kolpakov
V A
VA
Rekhter
M D
MD
Mironov
A A
AA
D000818:Animals; D004730:Endothelium, Vascular / Q000473:pathology*; D005404:Fixatives / Q000009:adverse effects; D005976:Glutaral; D006801:Humans; D051381:Rats; D016622:Silver Staining / Q000379:methods*
D005404:Fixatives; D005976:Glutaral
Modifitsirovannyĭ metod impregnatsii éndoteliia, udobnyĭ dlia patologoanatomicheskikh issledovaniĭ.