title: Red blood cell susceptibility to lipid peroxidation, membrane lipid composition, and antioxidant enzymes in continuous ambulatory peritoneal dialysis patients.
abstract: OBJECTIVE
  To investigate the overall susceptibility of red blood cells (RBC) to lipid peroxidation from patients on continuous ambulatory peritoneal dialysis (CAPD).
  
  
  METHODS
  The following parameters were measured: RBC malondialdehyde (MDA) production after oxidative stress with H2O2, RBC antioxidant enzymes glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), and RBC membrane lipid composition. The levels of plasma vitamin E and serum selenium were also assayed.
  
  
  METHODS
  Eleven patients on continuous ambulatory peritoneal dialysis. Twenty-one healthy blood donors of similar age were used as normal controls.
  
  
  RESULTS
  The MDA formation after H2O2 stimulation was normal in CAPD patients (0.79 +/- 0.1 mumol/gHb versus 0.78 +/- 0.1 in the control group). RBC from CAPD patients also showed a normal SOD activity, a more than adequate vitamin E status, and a peculiar pattern of membrane lipids, with reduced polyunsaturated fatty acids (p less than 0.001) and increased monounsaturated fatty acids (p less than 0.001). Both RBC GSH-Px activity, a selenium-dependent enzyme, and serum selenium levels were significantly lower in CAPD patients, and a significant positive correlation (r = 0.68; p less than 0.02) between the two parameters was found.
  
  
  CONCLUSIONS
  This study found a normal sensitivity to oxidant stress in RBC from a group of CAPD patients, despite an impaired GSH-Px activity. The peculiar lipid pattern of RBC membrane, characterized by reduced PUFA and increased MUFA content, may contribute, in addition to adequate SOD activity and vitamin E status, to normal RBC lipid peroxidation.
authors.affiliation: Institute of Medical Pathology, University of Verona, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Girelli
authors.forename: A
authors.initials: A
authors.lastname: Lupo
authors.forename: M T
authors.initials: MT
authors.lastname: Trevisan
authors.forename: O
authors.initials: O
authors.lastname: Olivieri
authors.forename: P
authors.initials: P
authors.lastname: Bernich
authors.forename: P
authors.initials: P
authors.lastname: Zorzan
authors.forename: A
authors.initials: A
authors.lastname: Bassi
authors.forename: A M
authors.initials: AM
authors.lastname: Stanzial
authors.forename: S
authors.initials: S
authors.lastname: Ferrari
authors.forename: R
authors.initials: R
authors.lastname: Corrocher
mesh_terms: D000368:Aged; D000740:Anemia / Q000097:blood / Q000209:etiology*; D004910:Erythrocyte Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D008563:Membrane Lipids / Q000032:analysis*; D010531:Peritoneal Dialysis, Continuous Ambulatory; D013482:Superoxide Dismutase / Q000097:blood*; D014511:Uremia / Q000150:complications / Q000628:therapy
chemical_list: D008563:Membrane Lipids; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase