L-ascorbic acid 2-phosphate enhances the production of type I and type III collagen peptides in cultured rabbit keratocytes.
We studied the effect of L-ascorbic acid 2-phosphate (P-Asc), a long-acting phosphate derivative of L-ascorbic acid, on the production and secretion of type I and type III collagen peptides in cultured rabbit keratocytes using immunohistochemistry and enzyme immunoassay. P-Asc enhanced production and secretion of these collagen peptides. Our observations support a therapeutic role for P-Asc in the repair of corneal stromal damage such as caused by corneal chemical burn.
Saika
S
S
Department of Ophthalmology, Wakayama Medical College, Japan.
Uenoyama
K
K
Hiroi
K
K
Ooshima
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D001667:Binding, Competitive; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003315:Cornea / Q000166:cytology* / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011347:Procollagen / Q000096:biosynthesis*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D011347:Procollagen; C011669:ascorbate-2-phosphate; D003094:Collagen; D001205:Ascorbic Acid