Synaptic contacts between CGRP-immunoreactive terminals and enkephalin-immunoreactive neurons in the central amygdaloid nucleus of the rat.
An immunoelectron microscopic method combined with immunofluorescence double staining was carried out to examine the relationship between calcitonin gene-related peptide (CGRP)-like immunoreactive (LI) axon terminals and enkephalin (ENK)-LI neurons in the central amygdaloid nucleus (Ce) of the rat. The latter method showed that many ENK-LI cell bodies are densely surrounded by CGRP-LI axons in the lateral subdivision of the Ce (CeL). After taking fluorescence micrographs, the immunoperoxidase technique was used to examine the CGRP-LI axonal profiles under an electron microscope. CGRP-LI terminals were frequently found to form axo-somatic synaptic contacts with ENK-LI neurons in the CeL.
Shimada
S
S
Second Department of Anatomy, Osaka University Medical School, Suita, Japan.
Inagaki
S
S
Narita
N
N
Takagi
H
H
D000679:Amygdala / Q000166:cytology / Q000648:ultrastructure*; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D004745:Enkephalins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008297:Male; D016253:Microscopy, Immunoelectron; D009411:Nerve Endings / Q000648:ultrastructure*; D009474:Neurons / Q000166:cytology / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000648:ultrastructure*
D004745:Enkephalins; D015740:Calcitonin Gene-Related Peptide