The double disector: unbiased stereological estimation of the number of particles inside other particles.
A double-disector method for obtaining estimates of the number of particles inside other particles, illustrated by an estimation of the average cell number in a glomerulus, has been developed. The method is suitable for paraffin-embedded tissue because it does not require knowledge about the section thickness. Most importantly, the estimate is absolutely unaffected by tissue shrinkage. The average number of cells in a human glomerulus is 2850 whereas in rats it is 580.
Marcussen
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University Institute of Pathology, University of Aarhus, Denmark.
D000818:Animals; D002452:Cell Count; D006801:Humans; D007678:Kidney Glomerulus / Q000166:cytology*; D008297:Male; D008853:Microscopy / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains