Glucose- and acetylcholine-induced increase in intracellular free Ca2+ in subpopulations of individual rat pancreatic beta-cells.
The effects of glucose and acetylcholine (ACh) on the intracellular free Ca2+ ion concentration ([Ca2+]i) were measured using fura-2 microspectrofluorimetry in individual rat pancreatic beta-cells prepared by enzymatic digestion and fluorescence-activated cell sorting. The average [Ca2+]i was 139 +/- 2.2 nM (n = 84) in the presence of 4.4 mM glucose. At 17.8 mM, glucose caused transient or sustained increases in [Ca2+]i in some individual beta-cells (15% of the total cells tested). However, the majority of glucose (17.8 mM)-nonresponsive cells responded to ACh, cholecystokinin-8, and K+. ACh at 10(-4) M stimulated increases in [Ca2+]i in most of the glucose-nonresponsive beta-cells in the presence of 4.4 mM glucose, and the effect was concentration dependent. High concentrations of glucose potentiated ACh-induced increases in [Ca2+]i observed in some of the glucose-nonresponsive beta-cells (glucose-sensitive cells), demonstrating that the function of some beta-cells is affected by the interaction of glucose with ACh. However, glucose did not affect the ACh-induced increase in [Ca2+]i in other glucose-nonresponsive cells (glucose-insensitive cells). These data strongly indicate that there are populations of beta-cells that exhibit different [Ca2+]i responses to glucose.
Wang
J
J
Medical Research Council Regulatory Peptide Group, University of British Columbia, Vancouver, Canada.
Baimbridge
K G
KG
Brown
J C
JC
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012844:Sincalide / Q000494:pharmacology
D005947:Glucose; D012844:Sincalide; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium