Autoradiographic determination of glucose content and estimation of the lumped constant in intracerebral neuronal tissue transplants.
The quasi-steady-state distribution volume of brain glucose was measured using 3-O-[14C]methylglucose quantitative autoradiography in a group of rats (n = 5) which 12-15 weeks previously had undergone unilateral ibotenic acid-induced lesion of the nucleus basalis magnocellularis, followed by implantation into ipsilateral neocortex of primordial basal forebrain cell suspensions. The effects of the lesion and the presence of transplanted tissue in neocortex were visualized by acetylcholinesterase histochemistry. The 3-O-[14C]methylglucose tracer was distributed homogeneously throughout the host brain areas analysed, with no side-to-side differences, despite a marked unilateral depletion of acetylcholinesterase activity ipsilateral to the lesion site. Whilst the transplants were indistinguishable from the homogeneity of surrounding host frontal cortex, there was a 70% increase in the apparent distribution volume of methylglucose localized around the ibotenate injection site and associated needle tract. Brain glucose content is an important experimental variable affecting the lumped constant of the 2-deoxyglucose technique. There was no evidence of any significant difference in the lumped constant between transplant and host tissue which might compromise the validity of the 2-deoxyglucose technique when used together with intracerebral implantation of fetal neuronal cell suspensions.
Kelly
P A
PA
Department of Clinical Neurosciences, University of Edinburgh, Western General Hospital, Scotland, U.K.
Sharkey
J
J
Ritchie
I M
IM
D019325:3-O-Methylglucose; D000818:Animals; D001345:Autoradiography / Q000379:methods; D016380:Brain Tissue Transplantation / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism* / Q000637:transplantation*; D007839:Functional Laterality; D005947:Glucose / Q000378:metabolism*; D007051:Ibotenic Acid / Q000633:toxicity; D008297:Male; D008757:Methylglucosides / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000637:transplantation; D016548:Prosencephalon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014316:Tritium
D008757:Methylglucosides; D014316:Tritium; D019325:3-O-Methylglucose; D007051:Ibotenic Acid; D005947:Glucose