Glycosylation of macrolide antibiotics in extracts of Streptomyces lividans.
Inducible resistance to macrolide antibiotics in Streptomyces lividans involves MGT, a macrolide glycosyl transferase that utilizes UDP-glucose as cofactor. Substrates for MGT include macrolides with 12-, 14-, 15-, or 16-atom cyclic polyketide lactones (as in methymycin, erythromycin, azithromycin, or tylosin, respectively), although spiramycin and carbomycin are not apparently modified. The enzyme is specific for the 2'-OH group of saccharidic moieties attached to C-5 of the 16-atom lactone ring (corresponding to C-5 or C-3 in 14- or 12-atom lactones, respectively).
Cundliffe
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Department of Biochemistry, University of Leicester, England.
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D003470:Culture Media; D004352:Drug Resistance, Microbial / Q000235:genetics; D005964:Glucosyltransferases; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D018942:Macrolides; D011815:R Factors; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013379:Substrate Specificity; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D000900:Anti-Bacterial Agents; D003470:Culture Media; D018942:Macrolides; D016695:Glycosyltransferases; D005964:Glucosyltransferases; C075034:macrolide glycosyltransferase; D014532:Uridine Diphosphate Glucose