Effects of L-histidine on hydrogen peroxide-induced DNA damage and cytotoxicity in cultured mammalian cells.
L-Histidine markedly increased the growth- and DNA synthesis-inhibitory effects elicited by hydrogen peroxide in cultured Chinese hamster ovary cells. DNA single-strand breakage was also higher in the presence of the amino acid and, in addition, these breaks were characterized by a slower rate of repair, compared with that of the breaks generated by the oxidant alone. In the presence of L-histidine, hydrogen peroxide also produced DNA double-strand breakage, a lesion that cannot be detected in cells treated with even exceedingly high concentrations of the oxidant alone. Data reported herein suggest that the L-histidine-mediated increase of the cytotoxic response of cultured Chinese hamster ovary cells to hydrogen peroxide may be at least partially dependent on the formation of DNA double-strand breaks.
Cantoni
O
O
Istituto di Farmacologia e Farmacognosia, Università degli Studi di Urbino, Italy.
Sestili
P
P
Guidarelli
A
A
Giacomoni
P U
PU
Cattabeni
F
F
D000818:Animals; D016466:CHO Cells; D002250:Carbon Radioisotopes; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006639:Histidine / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics
D002250:Carbon Radioisotopes; D006639:Histidine; D004247:DNA; D006861:Hydrogen Peroxide