Assessment of neutrophil chemotaxis by laser and video densitometry.
A laser densitometer and a video densitometer were used to evaluate neutrophil chemotaxis slides which were also assessed by the standard microscopic technique. A linear relationship was observed between cell number per high power field (HPF) and peak height by laser densitometry (r2 = 0.66) or peak area by video densitometry (r2 = 0.81). For wells containing more than 20 cells/HPF the least variability was observed with video densitometry. Quantitative results from neutrophil chemotaxis assays performed in 48-well microchambers can be obtained rapidly and conveniently by the use of commercially available video densitometers.
Misso
N L
NL
Department of Medicine, University of Western Australia.
Kang
T L
TL
Phillips
M J
MJ
Thompson
P J
PJ
D002634:Chemotaxis, Leukocyte; D003720:Densitometry / Q000379:methods*; D006801:Humans; D007834:Lasers; D009504:Neutrophils / Q000502:physiology; D012044:Regression Analysis