title: Electrospray ionization mass spectra of hemoglobin and transferrin by a magnetic sector mass spectrometer. Comparison with theoretical isotopic distributions.
abstract: Electrospray ionization mass spectra of human hemoglobin chains and of transferrin were acquired on a magnetic sector mass spectrometer. The observed molecular ion for each hemoglobin chain was in good agreement with the theoretical isotopic distribution at a reasonable resolution of 2000. The clear separation of a variant beta-chain in admixture with the normal counterpart at mass 15,867 that differed from it by 14 Da (0.09%) ensured that a smaller mass difference could be detected. The molecular ions for human transferrin were too broad compared with the theoretical shape to determine the molecular mass accurately, probably due to the heterogeneity of the carbohydrate moiety. A decrease in mass by neuraminidase digestion, however, determined the average number of sialic acids in the molecule.
authors.affiliation: Department of Molecular Medicine, Osaka Medical Center for Maternal and Child Health, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Wada
authors.forename: J
authors.initials: J
authors.lastname: Tamura
authors.forename: B D
authors.initials: BD
authors.lastname: Musselman
authors.forename: D B
authors.initials: DB
authors.lastname: Kassel
authors.forename: T
authors.initials: T
authors.lastname: Sakurai
authors.forename: T
authors.initials: T
authors.lastname: Matsuo
mesh_terms: D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D013058:Mass Spectrometry; D014168:Transferrin / Q000032:analysis*
chemical_list: D006454:Hemoglobins; D014168:Transferrin