title: Cytokinetic failure and asynchronous nuclear division in BHK cells overexpressing a truncated protein-tyrosine-phosphatase.
abstract: Previous work has shown that a T-cell protein-tyrosine-phosphatase truncated in its carboxyl-terminal domain (delta C11.PTP) has full enzymatic activity but no longer localizes in the particulate fraction of the cell. Two baby hamster kidney (BHK) cell lines overexpressing the truncated protein are markedly multinucleate, a state likely caused by a failure in cytokinesis. Nuclei within syncytial cells overexpressing delta C11.PTP display a remarkable asynchronous entry into mitosis. The effects require tyrosine phosphatase activity because expression of an inactive form of the truncated enzyme yields cells indistinguishable from the parental cell line. Redistribution of the enzyme from the particulate to the soluble fraction is apparently important to these observed effects because cells overexpressing the full-length, wild-type enzyme are morphologically similar to controls. Further, when these cells contain more than one nucleus, their syncytial nuclei undergo mitosis synchronously.
authors.affiliation: Department of Biochemistry, University of Washington, Seattle 98195.
authors.forename: D E
authors.initials: DE
authors.lastname: Cool
authors.forename: P R
authors.initials: PR
authors.lastname: Andreassen
authors.forename: N K
authors.initials: NK
authors.lastname: Tonks
authors.forename: E G
authors.initials: EG
authors.lastname: Krebs
authors.forename: E H
authors.initials: EH
authors.lastname: Fischer
authors.forename: R L
authors.initials: RL
authors.lastname: Margolis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure*; D006224:Cricetinae; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D008938:Mitosis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D010641:Phenotype; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014162:Transfection
chemical_list: D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins; D017027:Protein Tyrosine Phosphatases