Increased glycated Cu,Zn-superoxide dismutase levels in erythrocytes of patients with insulin-dependent diabetis mellitus.
Our previous study indicated that erythrocyte Cu,Zn-superoxide dismutase (Cu,Zn-SOD) undergoes glycation and inactivation in vivo (1) and in vitro (2). The aim of the present study was to assess glycated Cu,Zn-SOD in patients with insulin-dependent diabetes mellitus. Glycated Cu,Zn-SOD, which binds to a boronic acid affinity column, was measured by the enzyme-linked immunosorbent assay. The percentage of the glycated form in 25 insulin-dependent diabetic children was 40.2 +/- 8.2%; this was significantly higher than that in the normal controls (P less than 0.01). The specific activity of the glycated form in the diabetic children was 163,000 +/- 33,000 IU/mg Cu,Zn-SOD protein, significantly lower than that in controls (P less than 0.01). These data indicate that glycated and less active Cu,Zn-SOD is increased in erythrocytes of patients with insulin-dependent diabetes mellitus.
Kawamura
N
N
Department of Biochemistry, Osaka University Medical School, Japan.
Ookawara
T
T
Suzuki
K
K
Konishi
K
K
Mino
M
M
Taniguchi
N
N
D000328:Adult; D000367:Age Factors; D015415:Biomarkers / Q000097:blood; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D019270:Fructosamine; D006442:Glycated Hemoglobin / Q000032:analysis; D006031:Glycosylation; D006595:Hexosamines / Q000097:blood; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D007700:Kinetics; D012016:Reference Values; D013482:Superoxide Dismutase / Q000097:blood*
D015415:Biomarkers; D019270:Fructosamine; D006442:Glycated Hemoglobin; D006595:Hexosamines; D007527:Isoenzymes; D013482:Superoxide Dismutase