Identification of Casuarina-Frankia strains by use of polymerase chain reaction (PCR) with arbitrary primers.
Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers showed polymorphism in the eight different Frankia genomes. Analysis of the AP-PCR products showed 9 polymorphic bands ranging from 4.1-0.60 kb. We conclude that single arbitrary primers can be used to amplify genomic DNA, and that polymorphism can be detected between the amplification products of the different Frankia genomes.
Sellstedt
A
A
Department of Plant Physiology, University of Umeå, Sweden.
Wullings
B
B
Nyström
U
U
Gustafsson
P
P
D000192:Actinomycetales / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010944:Plants / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides