A naturally occurring peptide recognized by alloreactive CD8+ cytotoxic T lymphocytes in association with a class I MHC protein.
The antigenic structures that initiate T cell responses to foreign (allogeneic) cells have long attracted considerable interest. We have purified and sequenced a peptide from mouse spleen that is recognized in association with the class I MHC protein H-2Ld by 2C, an alloreactive CD8+ T cell clone. The peptide (LSP-FPFDL) greatly enhances the susceptibility of Ld+ cells to lysis by 2C, and this activity is completely blocked by a clonotypic antibody against the 2C T cell receptor. Thus, this study characterizes the naturally occurring peptide moiety of an MHC-I/peptide complex recognized by alloreactive CD8+ T cells. The peptide, which occurs in the thymus of MHC-disparate mice, can be used to study T cell development in mice expressing transgenes for the 2C T cell receptor.
Udaka
K
K
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Tsomides
T J
TJ
Eisen
H N
HN
D000595:Amino Acid Sequence; D000818:Animals; D016827:CD8 Antigens / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000378:metabolism*; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D011402:Pronase / Q000494:pharmacology; D011485:Protein Binding; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D016827:CD8 Antigens; D006183:H-2 Antigens; D010455:Peptides; D011948:Receptors, Antigen, T-Cell; D011402:Pronase