Conditions for protoplasting, regenerating, and transforming the calicheamicin producer, Micromonospora echinospora.
We report methods to generate protoplasts, to regenerate mycelia, and to transform Micromonospora echinospora. This actinomycete produces the unusual antitumor antibiotics, the calicheamicins. These protocols may be applied to other actinomycetes that have been difficult to transform. These methods also may facilitate the cloning of calicheamicin biosynthetic genes by genetic complementation.
Love
S F
SF
Department of Microbial Genetics, Lederle Laboratories, Pearl River, New York 10965.
Maiese
W M
WM
Rothstein
D M
DM
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis; D053281:Enediynes; D007613:Kanamycin Resistance / Q000235:genetics; D008847:Micromonospora / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010957:Plasmids; D011523:Protoplasts; D014169:Transformation, Bacterial
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; C055955:calicheamicin gamma(1)I