Configuration of the 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholest-24-enoic acid, an intermediate in the peroxisomal conversion of 3 alpha,7 alpha,12-trihydroxy-5 beta-cholestanoic acid to cholic acid in rat liver.
3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholest-24-enoic acid, formed in the peroxisomal oxidation of 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-CoA by the THCA-CoA oxidase in rat liver, was isolated and purified on reverse phase HPLC. The configuration of the C-24/25 double bond was determined to be trans (E) by using 1H-NMR spectrometry.
Farrants
A K
AK
Institute for Nutrition Research, School of Medicine, University of Oslo, Norway.
Andersen
G I
GI
Byström
S E
SE
D000818:Animals; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D019826:Cholic Acid; D002793:Cholic Acids / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D008830:Microbodies / Q000378:metabolism; D008968:Molecular Conformation; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D002793:Cholic Acids; C059108:3,7,12-trihydroxycholest-24-enoic acid; C003681:3,7,12-trihydroxycoprostanic acid; D002784:Cholesterol; D010088:Oxidoreductases; C066160:trihydroxycholestanoyl-CoA oxidase; D019826:Cholic Acid