Evaluation of polysaccharide, lipopolysaccharide, and beta-glucan antigens in gel immunodiffusion tests for brucellosis in cattle.
In Venezuela, 1,012 cattle sera were screened for their ability to precipitate Brucella melitensis 16M smooth-lipopolysaccharide (S-LPS), B melitensis B115 polysaccharide B (poly B), B abortus 1119-3 O-polysaccharide (PS), or B abortus 1119-3 cyclic 1,2 linked beta-D-glucan (beta-glucan) in an agar-gel immunodiffusion assay. These sera were previously classified as being Brucella abortus-infected, S-19-vaccinated, or negative after an assessment of historical records and results of 5 standard serologic tests. Most of the sera (85%) from infected cattle precipitated S-LPS, poly B, and PS. Serologic results for poly B and PS were identical. On the other hand, 13% of the sera from vaccinated cattle precipitated S-LPS, but none of these sera precipitated poly B or PS. It was concluded that purified PS can alternate with poly B as an antigen to differentiate sera of B abortus-infected from B abortus S-19-vaccinated cattle. None of these sera precipitated beta-glucan.
Lord
V R
VR
Instituto de Investigaciones Veterinarias, Centro Nacional de Investigaciones Agropecuarias CENIAP, Laboratorio de Brucellosis, Maracay, Venezuela.
Cherwonogrodzky
J W
JW
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D002002:Brucella / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis*; D002417:Cattle; D003168:Complement Fixation Tests; D005936:Glucans / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion; D008070:Lipopolysaccharides / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D005936:Glucans; D007106:Immune Sera; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial