[Construction and identification of vectors containing maize plasmid-like DNA S1 and reporter genes].
The reporter Gene GUS with CaMV 35S promoter was inserted into plasmid pBS[1] in reversal direction of integration, and two kinds of recombinant plasmids were obtained. These results were verified by restriction endonuclease analysis and southern hybridization. A piece of 10.8kb fragment containing NPT II reporter gene from digested pBI121.1 by BamHI and EcoRI was ligated together with 3.9 kb fragment of plasmid-like DNA S1. Then generate a recombinant plasmid pBIS5 was generated. These three vectors can be used to transform maize protoplasts. High frequency of transformation may be expected due to homology between plasmid-like DNA S1 and maize nuclear DNA.
Liu
Z
Z
Beijing Agricultural University.
He
Q
Q
Xie
Y
Y
Dai
J
J
Mi
J
J
D004247:DNA / Q000235:genetics*; D005796:Genes; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D004247:DNA