Induction of proliferin gene expression by diverse chemical agents that promote morphological transformation in C3H/10T1/2 cultures.
Anthralin, iodoacetic acid, BHT, Tween 60 and TPA induced cytoplasmic accumulation of transcripts of the proliferin gene family at or near effective concentrations for promotion of morphological transformation of C3H/10T1/2 cells. Conversely, non-promoters (4-alpha-phorbol, formic acid and methanol) were ineffective inducers. Compounds likely to promote transformation (nickel sulphate, benzyl peroxide and t-butyl hydroperoxide) were also effective and apparently selective, proliferin inducers in C3H/10T1/2 and primary murine fibroblasts. Proliferin induction may be a useful molecular marker to predict chemically-induced promotion of morphological transformation in C3H/10T1/2 cells.
Parfett
C L
CL
Mutagenesis Section, Health and Welfare Canada, Ottawa, Ontario.
D000818:Animals; D000875:Anthralin / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D051379:Mice; D008809:Mice, Inbred C3H; D011083:Polycyclic Compounds / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D011388:Prolactin; D012333:RNA, Messenger / Q000378:metabolism*; D001613:beta 2-Microglobulin / Q000378:metabolism
D002273:Carcinogens; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D007461:Iodoacetates; D011083:Polycyclic Compounds; D011136:Polysorbates; C000634106:Prl2c2 protein, mouse; D012333:RNA, Messenger; D001613:beta 2-Microglobulin; D002084:Butylated Hydroxytoluene; D011388:Prolactin; D000875:Anthralin; D019807:Iodoacetic Acid