Identification of a nodD-like gene in Frankia by direct complementation of a Rhizobium nodD-mutant.
Clones from a Frankia At4 gene bank were pooled into groups and mass conjugated into a nodD mutant of Rhizobium leguminosarum bv. viciae by triparental matings. When peas were inoculated with the pooled transconjugants, nodulation was observed. A plasmid, pAt2GX containing Frankia DNA, was isolated from bacteria recovered from these nodules. This plasmid was shown to complement a nodD mutant of R. leguminosarum bv. viciae. Thus pAt2GX contains a Frankia gene that is functionally equivalent to nodD of R. leguminosarum bv. viciae.
Chen
L M
LM
Laboratory of Molecular Genetics, Guangxi Agricultural College, Nanning, China.
Cui
Y H
YH
Qin
M
M
Wang
Y L
YL
Bai
X L
XL
Ma
Q S
QS
D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D009154:Mutation; D009235:Myxomycetes / Q000235:genetics*; D010957:Plasmids; D016961:Rhizobium leguminosarum / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C060773:NodD protein, Bacteria