Affinity of SSB/La nuclear antigen to small nuclear ribonucleic acids.
Anti-SSB/La antibody is one kind of antinuclear antibody and is found predominantly in patients with Sjögren's syndrome and systemic lupus erythematosus. SSB/La antigen is present in the nucleus as ribonucleoprotein in particle form and is composed of peptide and RNAs. SSB/La antigen can be partially purified through several sequential procedures including salt fractionation and ion exchange chromatography. SSB/La antigen is capable of binding to 4 RNAs from uninfected KB cells and 6 RNAs from Epstein-Barr virus transformed WiL2 cells. These RNAs can bind to SSB/La peptide in vitro and form ribonucleoprotein complexes which could be reprecipitated by anti-SSB/La antibody. SSB/La associated RNAs are labile and subjected to degradation easily. Anti-SSB/La antibody may be a useful probe to investigate the potential role of Epstein-Barr virus involvement in cutaneous pathology.
Lee
P P
PP
Department of Dermatology, University of Pittsburgh School of Medicine, PA.
Deng
J S
JS
D000974:Antibodies, Antinuclear; D001324:Autoantigens / Q000378:metabolism*; D001665:Binding Sites; D002460:Cell Line; D002472:Cell Transformation, Viral; D006801:Humans; D012342:RNA, Small Nuclear / Q000378:metabolism*; D012261:Ribonucleoproteins; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000378:metabolism; D000097585:SS-B Antigen
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins; D000097585:SS-B Antigen; C035357:SS-B antibodies