Different profiles of induced cotton effects of human and bovine lactoferrin by cibacron blue F3GA binding.
1. The properties of complex formation of lactoferrin with Cibacron Blue F3GA dye have been studied by circular dichroic spectral analyses. The Cotton effects were induced by the interaction of lactoferrin with the dye and occurred in the wavelength range from 300 to 450 nm. 2. The patterns of changes in circular dichroic spectra of lactoferrin induced by the dye were different in bovine and human lactoferrin. 3. Iron ions bound to lactoferrin affected the profiles of induced Cotton effects in human lactoferrin but not in bovine lactoferrin. 4. While the dye bound co-operatively to human apo-lactoferrin, such co-operativeness was not observed in iron-saturated human lactoferrin.
Shimazaki
K
K
Protein Chemistry Section, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Nitta
K
K
Sato
T
T
Tomimura
T
T
Tomita
M
M
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004396:Coloring Agents / Q000737:chemistry*; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry*; D011485:Protein Binding; D013045:Species Specificity; D014227:Triazines / Q000737:chemistry*
D004396:Coloring Agents; D014227:Triazines; C012842:Cibacron Blue F 3GA; D007781:Lactoferrin