Radiation-reduced hybrids for the myotonic dystrophy locus.
The myotonic dystrophy (DM) gene maps to the long arm of human chromosome 19 and is flanked by markers ERCC1 and D19S51. Also mapping to this region is the polio virus receptor gene (PVS). To produce more markers for this interval, we have constructed radiation-reduced hybrids by selecting for the retention of ERCC1 and for the loss of PVS. One of the cell lines produced has been characterized extensively and contains about 2 Mb of human DNA derived exclusively from chromosome 19, and includes ERCC1 and D19S51. Phage libraries constructed from DNA of this cell line have been screened and several new markers identified, including two for which cDNAs have been isolated. These represent candidate genes for DM. The new markers have also been used to extend the long-range restriction map of this region.
Brook
J D
JD
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02138.
Zemelman
B V
BV
Hadingham
K
K
Siciliano
M J
MJ
Crow
S
S
Harley
H G
HG
Rundle
S A
SA
Buxton
J
J
Johnson
K
K
Almond
J W
JW
D001483:Base Sequence; D002460:Cell Line / Q000528:radiation effects; D002874:Chromosome Mapping / Q000379:methods*; D002888:Chromosomes, Human, Pair 19; D004247:DNA / Q000235:genetics; D015723:Gene Library; D005799:Genes, Dominant; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D015994:Incidence; D008969:Molecular Sequence Data; D009223:Myotonic Dystrophy / Q000453:epidemiology / Q000235:genetics*; D015183:Restriction Mapping; D012641:Selection, Genetic
D005819:Genetic Markers; D004247:DNA