Combined use of silver staining of the retrograde tracer WGAapoHRP-Au and pre-embedding immunocytochemistry for electron microscopy: demonstration of dopaminergic terminals in synaptic contact with striatal neurons projecting to the substantia nigra in the rat.
We investigated the applicability of the pre-embedding immunoperoxidase technique to WGAapoHRP-Au retrograde tracing. After injection of the tracer into the substantia nigra of rat, the brain was fixed and cryostat sections were immunostained for dopamine. The sections were osmicated and silver-stained to amplify the colloidal gold particles. Products of both the immunoperoxidase staining and the silver staining could be detected and distinguished by electron microscopy at low magnification. The ultrastructure was so well preserved that synaptic characteristics could be investigated. Dopaminergic terminals were demonstrated to synapse with striatal neurons projecting to the substantia nigra.
Arai
R
R
Department of Anatomy, Shiga University of Medical Science, Otsu, Japan.
Kojima
Y
Y
Geffard
M
M
Kitahama
K
K
Maeda
T
T
D000818:Animals; D003102:Colloids; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D006046:Gold; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000378:metabolism*; D016622:Silver Staining; D013378:Substantia Nigra / Q000166:cytology / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure
D003102:Colloids; D011954:Receptors, Dopamine; D006046:Gold