title: Sealed reticulocyte ghosts. An experimental model for the study of Fe2+ transport.
abstract: Sealed right-side-out reticulocyte ghosts transported and accumulated iron offered as 59Fe(2+)-ascorbate (Km = 1.1 microM). The uptake of iron by ghosts presented the characteristics of a transporter-mediated process: it responded to osmotic challenge, the rate of transport increased when iron was present in the opposing side, and the transport rate showed the temperature dependence typical of membrane-mediated processes. The transport of iron was dependent on an associated influx of Cl- in order to keep electroneutrality. Other transition metals, such as Cu2+, Zn2+, and Co2+, inhibited the transport of Fe2+. The overall characteristics of the system make reticulocyte sealed ghosts a very useful model in determining the basic mechanisms of membrane iron transport.
authors.affiliation: Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago.
authors.forename: M T
authors.initials: MT
authors.lastname: Núñez
authors.forename: A
authors.initials: A
authors.lastname: Escobar
authors.forename: A
authors.initials: A
authors.lastname: Ahumada
authors.forename: M
authors.initials: M
authors.lastname: Gonzalez-Sepulveda
mesh_terms: D000818:Animals; D000838:Anions; D001692:Biological Transport; D000001:Calcimycin / Q000494:pharmacology; D002413:Cations, Divalent; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007501:Iron / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D009995:Osmosis; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D013696:Temperature
chemical_list: D000838:Anions; D002413:Cations, Divalent; D000001:Calcimycin; D007501:Iron