title: Parvalbumin and calbindin immunoreactivity in the rat brain: a double-immunolabelling method.
abstract: Parvalbumin and calbindin, two calcium binding proteins in the nervous system, are present in certain neuronal subpopulations. In the present study a method for a simultaneous demonstration of the both antigens was developed, which labels parvalbumin- and calbindin-containing structures in contrasting colours. A horseradish peroxidase-conjugated second antibody was used for the visualization of the monoclonal anticalbindin antibody, whereas the biotinylated anti-parvalbumin antibody was demonstrated by means of a biotin-streptavidine-alkaline phosphatase system. The method may be useful to classify neuronal populations and to study their morphological relationship.
authors.affiliation: Paul-Flechsig-Institute of Brain Research, Department of Neurochemistry, University of Leipzig, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Schwab
authors.forename: G
authors.initials: G
authors.lastname: Brückner
authors.forename: W
authors.initials: W
authors.lastname: Härtig
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology; D001710:Biotin / Q000276:immunology; D001923:Brain Chemistry; D064026:Calbindins; D005260:Female; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D010320:Parvalbumins / Q000032:analysis* / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D064030:S100 Calcium Binding Protein G / Q000032:analysis* / Q000276:immunology; D019809:Streptavidin
chemical_list: D001426:Bacterial Proteins; D064026:Calbindins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; D001710:Biotin; D019809:Streptavidin; D006735:Horseradish Peroxidase