[Detection of HLA-DR antigens using the ethidium bromide fluorescence test].
HLA-DR antigens were examined in peripheral lymphocytes by means of a one-colour fluorescent microcytotoxic test using ethidium-bromide. Evidence of HLA-DR antigens was equally reliable as the two-colour fluorescent test. Contrary to the latter, however, faint non-specific positive results were obtained in sera not containing HLA-DR antibodies. This is caused probably by the fact that ethidium-bromide penetrates into cells with a very slightly damaged membrane. This reduces substantially the possibility of introduction of the method into common practice.
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Ustav hematologie a krevní transfúze, Praha.
D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D004996:Ethidium; D005453:Fluorescence; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans
D006684:HLA-DR Antigens; D004996:Ethidium
K otázce průkazu HLA-DR antigenů ethidium-bromidovým fluorescencním testem.