title: In vivo regulation of matrix vesicle concentration and enzyme activity during primary bone formation.
abstract: In vivo regulation of matrix vesicles (MV) during primary bone formation was examined using tibial marrow ablation in rats as the experimental model. The effects of bone-bonding and nonbonding implants on the number of MV/micron 2 of matrix and the alkaline phosphatase (ALPase) and phospholipase A2 (PA2) activities of MV-enriched microsomes (MVEM) isolated from the healing bone were studied. MV concentration, ALPase, and PA2 were increased by bone-bonding implants by day 3 post-surgery; a similar effect was seen in the contralateral limb, but at a lower magnitude. Nonbonding implants had no effect at day 3 and decreased MV concentration and PA2 activity at later time points; the same behavior was observed in the contralateral limb. These results demonstrate that MVs are influenced in a differential manner by implant materials, both locally and systemically, and can be regulated during primary mineralization.
authors.affiliation: Hebrew University, Jerusalem.
authors.forename: Z
authors.initials: Z
authors.lastname: Schwartz
authors.forename: L
authors.initials: L
authors.lastname: Swain
authors.forename: J
authors.initials: J
authors.lastname: Sela
authors.forename: U
authors.initials: U
authors.lastname: Gross
authors.forename: D
authors.initials: D
authors.lastname: Amir
authors.forename: D
authors.initials: D
authors.lastname: Kohavi
authors.forename: C
authors.initials: C
authors.lastname: Muller-Mai
authors.forename: B
authors.initials: B
authors.lastname: Boyan
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D001857:Bone Matrix / Q000201:enzymology / Q000648:ultrastructure*; D002516:Ceramics; D008297:Male; D008861:Microsomes / Q000201:enzymology; D010012:Osteogenesis / Q000502:physiology*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D019736:Prostheses and Implants; D051381:Rats; D013193:Stainless Steel; D014025:Titanium
chemical_list: D001672:Biocompatible Materials; D013193:Stainless Steel; D014025:Titanium; D010741:Phospholipases A; D054467:Phospholipases A2; D000469:Alkaline Phosphatase