Decreased expression of neurotrophin-3 mRNA in the rat hippocampus following transient forebrain ischemia.
The expression profile of neurotrophin-3 (NT-3) mRNA in the rat hippocampus after forebrain ischemia was investigated by Northern blot and S1 nuclease protection analyses. The NT-3 transcripts in the hippocampus immediately decreased after ischemic insult, became undetectable within 3 h and remained at undetectable levels for at least 7 days. In contrast, the expression of c-fos and c-jun mRNA transiently increased both in the cerebral cortex and in the hippocampus. These results suggest that brain ischemia triggers dynamic changes in gene expression including a neurotrophic factor, which may cause functional and/or morphological changes of the neuronal network.
Takeda
A
A
Department of Applied Physiology and Molecular Biology, Tohoku University School of Medicine, Sendai, Japan.
Onodera
H
H
Yamasaki
Y
Y
Furukawa
K
K
Kogure
K
K
Obinata
M
M
Shibahara
S
S
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015870:Gene Expression; D016762:Genes, fos; D016758:Genes, jun; D006624:Hippocampus / Q000503:physiopathology*; D002546:Ischemic Attack, Transient / Q000503:physiopathology*; D008297:Male; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics*; D020933:Neurotrophin 3; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D016548:Prosencephalon / Q000503:physiopathology*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D014158:Transcription, Genetic
D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger