Fasting induced alterations in mitochondrial palmitoyl-CoA metabolism may inhibit adipocyte pyruvate dehydrogenase activity.
1. Adipocytes from fed and fasted (24 hr) groups of rats were fractionated into mitochondria, microsomes and plasma membranes. 2. Fasting significantly decreased the mitochondrial activity of palmitoyl-CoA synthetase, palmitoyl-CoA hydrolase, beta-oxidation and pyruvate dehydrogenase. 3. Fasting elevated intramitochondrial long-chain acyl-CoA. 4. Pyruvate dehydrogenase was inhibited 50% by addition of 30 microM palmitoyl-CoA. 5. Fasting-induced changes in palmitoyl-CoA metabolism may modulate pyruvate dehydrogenase activity in adipocyte mitochondria.
Moore
K H
KH
Department of Chemistry, Oakland University, Rochester, MI 48309-4401.
Dandurand
D M
DM
Kiechle
F L
FL
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D005215:Fasting / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000378:metabolism*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D011768:Pyruvate Dehydrogenase Complex; D010171:Palmitoyl Coenzyme A; D008274:Magnesium