Sex- and strain-related differences in megakaryocytopoiesis and platelet production in C3H and BALB/c mice.
In an effort to explain the different platelet production capabilities of male and female mice, megakaryocyte and platelet indices were measured on castrated male and oophorectomized female C3H and BALB/c mice, along with suitable intact controls. In agreement with our previous work, intact male BALB/c mice had higher platelet counts and percent incorporation of sulfur 35 into platelet values than did intact female BALB/c mice. Also, both intact BALB/c and C3H male mice had higher platelet counts than their castrated counterparts. Fewer femoral megakaryocytes were found in intact BALB/c and C3H male mice than in their female counterparts (p less than 0.05), but only BALB/c male mice had larger megakaryocytes than BALB/c female mice (p less than 0.0005). Castration caused increased numbers and decreased sizes of megakaryocytes (p less than 0.05) in both strains of mice, but oophorectomy did not change the characteristics of megakaryocytes in these mice. In all treatment groups, C3H mice had megakaryocytes with higher average deoxyribonucleic acid content than did BALB/c mice (p less than 0.0005), that is, BALB/c mice had greater percentages of 8N and 16N megakaryocytes than did C3H mice, but C3H mice had higher proportions of 32N and 64N megakaryocytes than did BALB/c mice (p less than 0.05 to p less than 0.0005). Although a difference in megakaryocyte ploidy was not detected between intact male and intact female C3H mice, BALB/c female mice had elevated percentages of low ploidy classes (8N) when compared with BALB/c male mice (p less than 0.005). Intact male C3H mice had higher percentages of 16N megakaryocytes (p less than 0.05) than did their neutered counterparts.(ABSTRACT TRUNCATED AT 250 WORDS)
McDonald
T P
TP
Swearingen
C J
CJ
Cottrell
M B
MB
Clift
R E
RE
Bryant
S E
SE
Jackson
C W
CW
D000818:Animals; D001772:Blood Cell Count; D001792:Blood Platelets / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D004247:DNA / Q000032:analysis / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006410:Hematopoiesis; D008297:Male; D008533:Megakaryocytes / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C / Q000097:blood*; D008809:Mice, Inbred C3H / Q000097:blood*; D009919:Orchiectomy; D010052:Ovariectomy; D011003:Ploidies; D012727:Sex Characteristics; D013045:Species Specificity; D013455:Sulfur / Q000378:metabolism; D013462:Sulfur Radioisotopes
D013462:Sulfur Radioisotopes; D013455:Sulfur; D004247:DNA