title: Use of a direct enzyme-linked antiglobulin test for laboratory diagnosis of immune-mediated hemolytic anemia in dogs.
abstract: Detection of autoantibody, complement, or both bound to RBC is an essential requirement for unequivocal diagnosis of immune-mediated hemolytic anemia in dogs. An enzyme-linked antiglobulin test was adapted for laboratory diagnosis of this disease. The refinement and routine use of this assay have allowed further observation of the pathogenesis of the disease process. In particular, degree of hemolysis can be related to the degree of RBC sensitization associated with primary immune-mediated hemolytic anemia, and this correlation is highest for IgG autoantibody. Results indicate that autoantibody isotype might have an important role in the hemolytic process.
authors.affiliation: Department of Veterinary Medicine, University of Bristol, Langford, UK.
authors.forename: D R
authors.initials: DR
authors.lastname: Jones
authors.forename: T J
authors.initials: TJ
authors.lastname: Gruffydd-Jones
authors.forename: C R
authors.initials: CR
authors.lastname: Stokes
authors.forename: F J
authors.initials: FJ
authors.lastname: Bourne
mesh_terms: D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D000818:Animals; D003176:Complement C3 / Q000032:analysis*; D003298:Coombs Test / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D012016:Reference Values
chemical_list: D003176:Complement C3; D006454:Hemoglobins; D007074:Immunoglobulin G; D007075:Immunoglobulin M