Unique structure in the intergenic and 5' external transcribed spacer of the ribosomal RNA gene from the pea aphid Acyrthosiphon pisum.
We analyzed the DNA sequence of the 5' external transcribed spacer (ETS) and part of the intergenic transcribed spacer (IGS) of the aphid ribosomal RNA gene (rDNA). The 5' ETS of aphid rDNA consists of 843 nucleotides with a G/C content of 69 mol/100 mol, far higher than that of any other known 5' ETS for insect rDNA. The IGS of aphid rDNA contained a characteristic array of repeated sequences of 247 nucleotides. The repeated sequences were identical. It was shown that the number of the repeating sequence is heterogeneous.
Kwon
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Zoological Institute, Faculty of Science, University of Tokyo, Japan.
Ishikawa
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D000818:Animals; D001042:Aphids / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D004275:DNA, Ribosomal / Q000737:chemistry*; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal