title: Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells.
abstract: We earlier discovered a novel 40-kDa protein (hsp40) induced by heat shock and other stresses in mammalian and avian cells. In this report, we purified the hsp40 in HeLa cells, using modified two-dimensional gel electrophoresis, and determined the amino terminal amino acid sequence of this protein. The hsp40 is homologous to DnaJ, an Escherichia coli heat-shock protein, as well as to DnaJ-homologous proteins in yeast such as SCJ1, Sec63/Np11, YDJ1 and SIS1. Indirect immunofluorescence staining using an anti-hsp40 polyclonal antibody demonstrated that hsp40 was localized faintly throughout the cell in non-heat-shocked cells and was accumulated in nuclei and nucleoli in heat-shocked cells. The intracellular localization of hsp40 was very similar to that of hsp70, suggesting that these two hsps colocalize in heat-shocked HeLa cells.
authors.affiliation: Department of Oral Surgery, Nagoya University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hattori
authors.forename: Y C
authors.initials: YC
authors.lastname: Liu
authors.forename: I
authors.initials: I
authors.lastname: Tohnai
authors.forename: M
authors.initials: M
authors.lastname: Ueda
authors.forename: T
authors.initials: T
authors.lastname: Kaneda
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Tanabe
authors.forename: K
authors.initials: K
authors.lastname: Ohtsuka
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000737:chemistry* / Q000648:ultrastructure; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D006358:Hot Temperature; D006801:Humans; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000906:Antibodies; D006360:Heat-Shock Proteins