T-cell-dependent popliteal lymph node reactions to platinum compounds in mice.
The requirements for sensitization to complex salts of platinum were investigated in a mouse model by means of the popliteal lymph node (PLN) assay. A single subcutaneous injection of dissolved hexachloroplatinates without adjuvant induced a vigorous primary immune reaction in the draining PLN. Dose-dependent lymph node activation was determined by an increase in both PLN weight and cellularity. In C57BL/6 mice, peak reactions were obtained around day 6 after administration of 90-180 nmol Na2[PtCl6] or (NH4)2[PtCl6] per animal. Mice primed to [PtCl6]2- mounted an enhanced response upon local restimulation with suboptimal doses of the same but not unrelated compounds, indicating a specific secondary response. T cells were required to elicit PLN reactions to [PtCl6]2-, because athymic nude mice completely failed to respond, in contrast to their +/nu littermates. Differences between various inbred strains of mice revealed that Pt-induced PLN responses are genetically controlled. Moreover, the immunogenicity of Pt salts in mice is not confined to hexachloroplatinates, but other compounds, such as the antineoplastic agent cis-dichlorodiamine platinum, are able to induce comparable PLN reactions.
Schuppe
H C
HC
Department of Dermatology, Heinrich Heine University, Düsseldorf, FRG.
Haas-Raida
D
D
Kulig
J
J
Bömer
U
U
Gleichmann
E
E
Kind
P
P
D000818:Animals; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D004342:Drug Hypersensitivity / Q000276:immunology*; D007111:Immunity, Cellular / Q000235:genetics; D007279:Injections, Subcutaneous; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000033:anatomy & histology / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D009929:Organ Size; D010984:Platinum / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects*; D013997:Time Factors
D010984:Platinum