Cloning and expression of the human 5-HT1B-type receptor gene.
We report the cloning and expression of a novel 5-HT receptor gene from human genomic DNA. This clone, HGCR1, contains an apparently intronless open reading frame of 390 amino acids with the seven hydrophobic regions, typical of G-protein coupled receptors. The deduced amino acid sequence of HGCR1 is 39%, 55% and 87% identical to that for the human 5-HT1A, the human 5-HT1D and the rat 5-HT1B receptor, respectively. [3H]5-HT binding to transfected COS-7 cell membranes yields a pharmacological profile similar to that of 5-HT1B receptor. Thus these findings indicate the presence of 5-HT1B-type receptor in the human.
Mochizuki
D
D
Laboratory for Chemical Research, Asahi Chemical Industry, Shizuoka, Japan.
Yuyama
Y
Y
Tsujita
R
R
Komaki
H
H
Sagai
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D011985:Receptors, Serotonin / Q000145:classification / Q000235:genetics*; D016415:Sequence Alignment
D008565:Membrane Proteins; D011985:Receptors, Serotonin