title: Pathology of radiation injury to the canine spinal cord.
abstract: The histopathologic response of the canine spinal cord to fractionated doses of radiation was investigated. Forty-two dogs received 0, 44, 52, 60, or 68 Gy in 4 Gy fractions to the thoracic spinal cord. Dogs were evaluated for neurologic signs and were observed for 1 or 2 years after irradiation. Six major lesion types were observed; five in the irradiated spinal cord and one in irradiated dorsal root ganglia. The three most severe spinal cord lesions were white matter necrosis, massive hemorrhage, and segmental parenchymal atrophy which had an ED50 of 56.9 Gy (51.3-63.3 Gy 95% CI) in 4 Gy fractions. These lesions were consistently associated with abnormal neurologic signs. Radiation damage to the vasculature was the most likely cause of these three lesions. The two less severe spinal cord lesions were focal fiber loss, which had an ED50 of 49.5 Gy (44.8-53.6 Gy 95% CI) in 4 gy fractions and scattered white matter vacuolation that occurred at all doses. These less severe lesions were not consistently associated with neurologic signs and indicated the presence of residual damage that may occur after lower doses of radiation. Radiation damage to glial cells, axons, and/or vasculature were possible causes of these lesions. In the irradiated dorsal root ganglia, affected sensory neurons contained large intracytoplasmic vacuoles, and there was loss of neurons and satellite cells. Such alterations could affect sensory function. The dog is a good model for spinal cord irradiation studies as tolerance doses for lesions causing clinical signs are close to the estimated tolerance doses for humans, and studies involving volume and long-term observation can be done.
authors.affiliation: Department of Radiological Health Sciences, Colorado State University, Fort Collins 80523.
authors.forename: B E
authors.initials: BE
authors.lastname: Powers
authors.forename: E R
authors.initials: ER
authors.lastname: Beck
authors.forename: E L
authors.initials: EL
authors.lastname: Gillette
authors.forename: D H
authors.initials: DH
authors.lastname: Gould
authors.forename: R A
authors.initials: RA
authors.lastname: LeCouter
mesh_terms: D000818:Animals; D001284:Atrophy; D004285:Dogs; D004307:Dose-Response Relationship, Radiation; D006470:Hemorrhage / Q000209:etiology; D009336:Necrosis; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D013116:Spinal Cord / Q000528:radiation effects*