title: Heat shock protein Hsp60-reactive gamma delta cells: a large, diversified T-lymphocyte subset with highly focused specificity.
abstract: Previously, we detected a subset of gamma delta T cells in the newborn mouse thymus that responded to the mycobacterial heat shock protein Hsp60, as well as with what seemed to be a self-antigen. All of these cells expressed V gamma 1, most often in association with V delta 6+. It was not clear, however, whether similar, mature gamma delta cells with Hsp60 reactivity are common outside of the thymus, or rather, whether they are largely eliminated during development. From the data presented here, we estimate that gamma delta cells responding to Hsp60 comprise 10-20% of normal splenic and lymph node gamma delta T cells. Such cells, derived from adult spleen, always express a V gamma 1-J gamma 4-C gamma 4 gamma chain, although not all cells with this gamma chain show Hsp60 reactivity. Many of these V gamma 1+ cells also express V delta 6-J delta 1-C delta, though fewer than in V gamma 1+ cells from the newborn thymus. Extensive diversity is evident in both the gamma and delta chain junctional amino acids of the receptors of these cells, indicating that they may largely develop in the thymus of older animals or undergo peripheral expansion. Finally, we found that all such cells responding to both a putative self-antigen and to mycobacterial Hsp60 respond to a 17-amino acid synthetic peptide representing amino acids 180-196 of the Mycobacterium leprae Hsp60 sequence. This report demonstrates that a large subset of Hsp60-reactive peripheral lymphoid gamma delta T cells preexists in normal adult mice, all members of which respond to a single segment of this common heat shock protein.
authors.affiliation: Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
authors.forename: R L
authors.initials: RL
authors.lastname: O'Brien
authors.forename: Y X
authors.initials: YX
authors.lastname: Fu
authors.forename: R
authors.initials: R
authors.lastname: Cranfill
authors.forename: A
authors.initials: A
authors.lastname: Dallas
authors.forename: C
authors.initials: C
authors.lastname: Ellis
authors.forename: C
authors.initials: C
authors.lastname: Reardon
authors.forename: J
authors.initials: J
authors.lastname: Lang
authors.forename: S R
authors.initials: SR
authors.lastname: Carding
authors.forename: R
authors.initials: R
authors.lastname: Kubo
authors.forename: W
authors.initials: W
authors.lastname: Born
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D002459:Cell Fusion; D002460:Cell Line; D005803:Genes, Immunoglobulin; D006360:Heat-Shock Proteins / Q000138:chemical synthesis / Q000276:immunology / Q000494:pharmacology*; D006825:Hybridomas / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007376:Interleukin-2 / Q000032:analysis; D008198:Lymph Nodes / Q000276:immunology; D046911:Macromolecular Substances; D051379:Mice; D008810:Mice, Inbred C57BL; D009166:Mycobacterium leprae / Q000276:immunology*; D009838:Oligodeoxyribonucleotides; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D014158:Transcription, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D006360:Heat-Shock Proteins; D007135:Immunoglobulin Variable Region; D007376:Interleukin-2; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D011948:Receptors, Antigen, T-Cell