Gas chromatographic/mass spectrometric analysis of high-performance liquid chromatographic fractions reflecting arachidonic acid metabolism in mouse peritoneal macrophages.
Mouse peritoneal macrophages are used as a model for studies undertaken around the oxidative metabolism of arachidonic acid elicited by xenobiotics (N-phenyllinoleamide, related to the toxic oil syndrome, has been used as an example). A high-performance liquid chromatographic method for cyclo- and lipoxygenase metabolite fractionation has been developed. Gas chromatographic/mass spectrometric analysis of the high-performance liquid chromatographic fractions thus obtained show that the major products detected in the incubates correspond to three principal structures: monohydroxy acids (12-hydroxyeicosatetraenoic acid being the major component), epoxyhydroxy acids and trihydroxy acids. Other minor compounds such as 12-hydroxyheptadecatrienoic acid, various dihydroxy acids and prostaglandins were also detected. Cells pre-exposed to N-phenyllinoleamide show selectively enhanced levels of 6-keto prostaglandin F1 alpha, as measured by both gas chromatography/mass spectrometry and radioimmunoassay of the corresponding high-performance liquid chromatographic fraction.
Abián
J
J
Department of Neurochemistry (Eicosanoid Branch), CID-CSIC, Barcelona, Spain.
Bioque
G
G
Bulbena
O
O
Roselló
J
J
Gelpí
E
E
D000813:Anilides / Q000633:toxicity; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D008041:Linoleic Acids / Q000633:toxicity; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D011453:Prostaglandins / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D015262:Xenobiotics / Q000633:toxicity
D000813:Anilides; D005227:Fatty Acids; D008041:Linoleic Acids; D011453:Prostaglandins; D015262:Xenobiotics; C039294:linoleylanilide; D016718:Arachidonic Acid