The evaluation of 186Re-labeled antibodies using N2S4 chelate in vitro and in vivo using tumor-bearing nude mice.
We have recently described a method for radiolabeling monoclonal antibodies, with metallic radionuclides using a new chelating agent N2S3. Using this chelate the monoclonal antibodies Lym-1 and B72.3 were labeled with 186Re and their biological integrity was evaluated in vitro and in vivo. 186Re-labeled antibodies using N2S4 methodology were found to be stable in human serum and retained their immunoreactivity. Intravenous administration of 0.5 mCi 186Re-labeled antibodies resulted in partial or complete regression of tumor tissue in mice.
Najafi
A
A
Department of Radiology, USC School of Medicine, Los Angeles 90033.
Alauddin
M M
MM
Sosa
A
A
Ma
G Q
GQ
Chen
D C
DC
Epstein
A L
AL
Siegel
M E
ME
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D002051:Burkitt Lymphoma / Q000276:immunology / Q000532:radiotherapy; D005029:Ethylenediamines / Q000737:chemistry*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D000922:Immunotoxins / Q000627:therapeutic use*; D007553:Isotope Labeling / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology / Q000532:radiotherapy*; D016499:Radioimmunotherapy; D011868:Radioisotopes / Q000627:therapeutic use*; D012211:Rhenium / Q000627:therapeutic use*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D005029:Ethylenediamines; D000922:Immunotoxins; D011868:Radioisotopes; D013438:Sulfhydryl Compounds; C068737:tetrakis(2-mercaptoethyl)ethylenediamine; D012211:Rhenium