Cyclophosphamide-induced neutropenia: effect on postischemic skin-flap survival.
In a blinded study, 24 pigs were randomized to a 5-day preoperative treatment regimen of cyclophosphamide (n = 12) or placebo (n = 12). At operation, buttock cutaneous and latissimus dorsi myocutaneous flaps were created and then subjected to 6 hours of global ischemia. After 24 hours of reperfusion, flap skin and muscle survivals were determined. All cyclophosphamide-treated animals were rendered neutropenic (less than 500 neutrophils/mm3 of peripheral blood). The results show that neutropenia had no effect on postischemic buttock cutaneous flap survival. In contrast, cyclophosphamide-induced neutropenia demonstrated a significant protective effect on postischemic latissimus dorsi myocutaneous flap survival. This study further implicates the neutrophil as a significant factor in the mediation of ischemia/reperfusion injury of myocutaneous flaps.
Lee
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C
Microsurgical Research Laboratory, Royal Victoria Hospital, Montreal, P.Q., Canada.
Kerrigan
C L
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Picard-Ami
L A
LA
D000704:Analysis of Variance; D000818:Animals; D003520:Cyclophosphamide; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D007511:Ischemia / Q000503:physiopathology*; D009336:Necrosis; D009503:Neutropenia / Q000139:chemically induced; D009504:Neutrophils / Q000502:physiology*; D011897:Random Allocation; D016037:Single-Blind Method; D012867:Skin / Q000098:blood supply / Q000473:pathology; D013524:Surgical Flaps / Q000473:pathology / Q000502:physiology*; D013552:Swine
D003520:Cyclophosphamide