Sex determination of forensic samples by dual PCR amplification of an X-Y homologous gene.
Sex determination by polymerase chain reaction (PCR) analysis of the X-Y homologous amelogenin gene is highly reliable since the detection of an X-specific amplified fragment validates the procedure. Previously, we reported that 250 ng of template DNA are required for sex determination by this method. We report here a refinement of the technique to include dual PCR. Dual PCR using two sets of primers results in the detection of X- and Y-specific amplified fragments from as little as 0.005 ng of template DNA. This is a powerful technique for the analysis of trace forensic samples and its application is discussed.
Akane
A
A
Department of Legal Medicine, Shimane Medical University, Izumo, Japan.
Seki
S
S
Shiono
H
H
Nakamura
H
H
Hasegawa
M
M
Kagawa
M
M
Matsubara
K
K
Nakahori
Y
Y
Nagafuchi
S
S
Nakagome
Y
Y
D053523:Amelogenin; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D003746:Dental Enamel Proteins / Q000235:genetics*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D005784:Gene Amplification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012730:Sex Chromosomes / Q000737:chemistry*; D012732:Sex Determination Analysis; D013698:Templates, Genetic; D014960:X Chromosome / Q000737:chemistry; D014998:Y Chromosome / Q000737:chemistry
D053523:Amelogenin; D003746:Dental Enamel Proteins; D004247:DNA