title: [Serological confirmation of HIV infections in hospital practice: analysis of indeterminate western blot results].
abstract: Among 770 Western blots for HIV-1 confirmation on sera from subjects at high risk for HIV infection, 4.3% (33 cases) were indeterminate. Isolated, stable, reproducible anti-gp 160 reactivity, highly suggestive of a nonspecific reaction, was found in 16% of cases. There were three other probably nonspecific patterns with anti-gp 160 and either anti-gp 41 or anti-gp 120 reactivities and thin, atypical bands. Two patterns with anti-p24 and either anti-gp 160 or anti-gp 120 reactivities were consistent with HIV-1 seroconversion. Reactivity directed solely against gag products was seen in 18% of cases. The repeat test, performed in 16 cases, showed an identical pattern in 3 cases, a modified pattern in 3 cases, negative results in 9 cases, and seroconversion in 1 case. No case of HIV-2 infection was detected. Indeterminate Western blot results reflect nonspecific reactivity in most instances but should nevertheless lead to the exclusion of technical artefacts, seroconversion, and HIV-2 infection.
authors.affiliation: Service de Microbiologie, Hôpital Broussais, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Bottier
authors.forename: B
authors.initials: B
authors.lastname: Caroff
authors.forename: P
authors.initials: P
authors.lastname: Goldschmidt
authors.forename: F X
authors.initials: FX
authors.lastname: Mbopi Keou
authors.forename: S
authors.initials: S
authors.lastname: Cotigny
authors.forename: F M
authors.initials: FM
authors.lastname: Matta
authors.forename: M F
authors.initials: MF
authors.lastname: Longuet
authors.forename: L
authors.initials: L
authors.lastname: Bélec
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology / Q000517:prevention & control*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015497:HIV-1 / Q000302:isolation & purification*; D015498:HIV-2 / Q000302:isolation & purification*; D006757:Hospital Units; D006801:Humans; D008297:Male; D008403:Mass Screening; D012189:Retrospective Studies
vernacular_title: Sérologie de confirmation de l'infection par le VIH en pratique hospitalière: analyse des western blots indéterminés.