Expression of ORF1 of the linear 2.3 kb plasmid of maize mitochondria: product localization and similarities to the 130 kDa protein encoded by the S2 episome.
ORF1 of the ubiquitous 2.3 kb linear plasmid of maize mitochondria encodes a 39 kDa protein detected with polyclonal antibodies raised to a beta-galactosidase:ORF1 fusion protein. Almost half of this protein is similar to a domain of the 130 kDa protein encoded by the S2 episome of mitochondria from cytoplasmic male-sterile lines. Antisera raised to the ORF1 2.3 kb plasmid product cross-reacts with the ORF1, 130 kDa protein from the S2 episome. Despite the shared domain, the proteins are differentially localized: the 130 kDa protein is membrane-associated while the ORF1 protein is found in the matrix. We discuss possible functions of the ORF1 protein.
Leon
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P
Department of Biological Sciences, Stanford University, CA 94305-5020.
O'Brien-Vedder
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Walbot
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000276:immunology; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D003313:Zea mays / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics
D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase