A trisialoganglioside containing a sialyl alpha 2-6 N-acetylgalactosamine residue is a cholinergic-specific antigen, Chol-1 alpha.
Cholinergic-specific antigens termed the Chol-1 family have been suggested to be of a ganglioside nature by Richardson et al. (J. Neurochem. 38, 1605-1614, 1982). Two molecular species of polysialogangliosides among bovine brain gangliosides were found to react with anti-Chol-1 alpha antiserum. One of them, Chol-1 alpha-a, was isolated and characterized as a trisialoganglioside containing the gangliotetraose backbone in which 1 mol of sialic acid was attached to each of the reducing end galactose, N-acetylgalactosamine and internal galactose residues, respectively. The chemical structure of Chol-1 alpha-a was determined for the first time, being as follows: IV3NeuAc III6NeuAc II3NeuAc-GgOse4 Cer.
Ando
S
S
Department of Membrane Biochemistry, Tokyo Metropolitan Institute of Gerontology.
Hirabayashi
Y
Y
Kon
K
K
Inagaki
F
F
Tate
S
S
Whittaker
V P
VP
D000954:Antigens, Surface / Q000032:analysis*; D002799:Cholinergic Fibers / Q000276:immunology*; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000032:analysis*; D013058:Mass Spectrometry; D009411:Nerve Endings / Q000276:immunology
D000954:Antigens, Surface; D005732:Gangliosides; C038777:chol-1 antigen; C016806:trisialoganglioside