title: Determination of aminoglycoside antibiotics by reversed-phase ion-pair high-performance liquid chromatography coupled with pulsed amperometry and ion spray mass spectrometry.
abstract: This work constitutes a preliminary investigation of a high-performance liquid chromatographic (HPLC)-mass spectrometric (MS) method for confirming aminoglycoside residues in bovine tissues. A reversed-phase ion-pair HPLC method for the separation of four aminoglycosides was developed using volatile ion-pairing agents and optimized for detection with an ion spray HPLC-MS interface. The method is also compatible with a commercial pulsed amperometric detector that was used for HPLC method development and that may be useful for the screening and quantification phases of a regulatory method. Several column phases, eluent compositions, and pairing ions were evaluated for optimum HPLC-MS sensitivity. Detection limits are in the low nanogram range with the pulsed amperometric detector and with HPLC-MS in the selected ion monitoring mode. Results with bovine kidney, fortified to 20 ppm and extracted by matrix solid-phase dispersion, obtained using both detectors are presented.
authors.affiliation: Drug Testing and Toxicology, NYS College of Veterinary Medicine, Cornell University, Ithaca 14850.
authors.forename: L G
authors.initials: LG
authors.lastname: McLaughlin
authors.forename: J D
authors.initials: JD
authors.lastname: Henion
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D007477:Ions