title: Electron microscopical and immunohistochemical studies on the induction of "Qi" employing needling manipulation.
abstract: During a sparrow-pecking and twisting-needle manipulation to the acupoints BL 23, 24 and 25 for an induction of "Qi", it was found that some transparent materials were binding to the needles after removed from the volunteer's skin. Electron-microscopical analysis of the transparent materials revealed that they corresponded to the injured fascia made up of collagen fibers, elastic fibers, fibroblasts, adipocytes and mast cells. Rarely were nerve fiber-like structures observed in the materials. Immunohistochemically, calcitonin gene-related peptide-positive nerve fibers could be demonstrated in the acupoint BL 24 associated fascia. A possible functional relationship between the needle manipulation and the induction of Qi-sensation is discussed along with the acupoint tissue constitution.
authors.affiliation: Kansai College of Acupuncture Medicine, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Tohya
authors.forename: K
authors.initials: K
authors.lastname: Kuroiwa
authors.forename: H
authors.initials: H
authors.lastname: Oda
authors.forename: E C
authors.initials: EC
authors.lastname: Gorawski
authors.forename: Z X
authors.initials: ZX
authors.lastname: Hua
authors.forename: S
authors.initials: S
authors.lastname: Toda
authors.forename: M
authors.initials: M
authors.lastname: Ohnishi
authors.forename: E
authors.initials: E
authors.lastname: Noguchi
mesh_terms: D015669:Acupuncture Points; D015670:Acupuncture Therapy / Q000009:adverse effects / Q000379:methods* / Q000592:standards; D000328:Adult; D000818:Animals; D005069:Evaluation Studies as Topic; D005205:Fascia / Q000276:immunology / Q000293:injuries* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D051381:Rats; D012867:Skin / Q000276:immunology / Q000293:injuries* / Q000648:ultrastructure