title: Clearance of 99mTc-DTPA and experimentally increased alveolar surfactant content.
abstract: We measured clearance of 99mTc-labeled diethylenetriamine pentaacetic acid (99mTc-DTPA) in rabbits with experimentally increased alveolar surfactant content. In one group of animals, surfactant production was increased by treatment with ambroxol, and another group of animals was treated with tracheal instillation of natural surfactant. A group of untreated control animals and animals treated with instillation of saline were also studied. Clearance was measured during standard conditions of mechanical ventilation and during ventilation with large tidal volumes. In ambroxol- and surfactant-treated groups, clearance rate was reduced compared with untreated control animals. In contrast, clearance rate increased after saline instillation. The differences were observed at both modes of ventilation. The findings indicate that the pulmonary surfactant system is a rate-limiting factor for the clearance of 99mTc-DTPA and that the volume dependence of clearance is not explained by stretching of the alveolar wall only.
authors.affiliation: Department of Anaesthesiology, Erasmus University, Rotterdam, The Netherlands.
authors.forename: J A
authors.initials: JA
authors.lastname: Bos
authors.forename: P
authors.initials: P
authors.lastname: Wollmer
authors.forename: W
authors.initials: W
authors.lastname: Bakker
authors.forename: E
authors.initials: E
authors.lastname: Hannappel
authors.forename: B
authors.initials: B
authors.lastname: Lachmann
mesh_terms: D000551:Ambroxol / Q000494:pharmacology; D000818:Animals; D006207:Half-Life; D008657:Metabolic Clearance Rate; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism*; D011817:Rabbits; D012121:Respiration, Artificial; D016284:Technetium Tc 99m Pentetate / Q000493:pharmacokinetics*; D013990:Tidal Volume / Q000502:physiology
chemical_list: D011663:Pulmonary Surfactants; D000551:Ambroxol; D016284:Technetium Tc 99m Pentetate