Inhibition of penta-acetyl geniposide on AFB1-induced genotoxicity in C3H10T1/2 cells.
A new compound, penta-acetyl geniposide ((Ac)5-GP), was obtained from modified extract of Gardenia fructus (San-Jee-Chee in Chinese). The structure of the compound was identified as 1-(beta-D-2',3',4',6'-tetraacetyl-glucopyrannosyloxyl)-1,4a, 5,7a-tetrahydro-7-(acetomethyl)-cyclopentapyran-4-carboxylic acid methyl ester, according to the spectral data. The inhibitory effects of (Ac)5-GP on aflatoxin B1 (AFB1)-induced cytotoxicity and DNA damage were studied. In the investigation of the inhibitory effect of (Ac)5-GP on AFB1-cytotoxicity, the plating efficiency of C3H10T1/2 cells in S-9 activation system was increased. In addition, (Ac)5-GP inhibited the DNA damage of AFB1-treated C3H10T1/2 cells, and it interfered with the inhibitory effect of DNA synthesis caused by AFB1. These results suggest that the reduced DNA damage and the increased DNA synthesis from cultured C3H10T1/2 cells are important mechanisms for the inhibition of AFB1-cytotoxicity by (Ac)5-GP.
Tseng
T H
TH
Department of Biochemistry, Chung Shan Medical and Dental College, Taichung, Taiwan, Republic of China.
Chu
C Y
CY
Wang
C J
CJ
D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors* / Q000633:toxicity; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D057888:Iridoid Glucosides; D039823:Iridoids; D051379:Mice; D008809:Mice, Inbred C3H; D010946:Plants, Medicinal; D011714:Pyrans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D016587:Antimutagenic Agents; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; C074968:pentaacetyl geniposide; D016604:Aflatoxin B1