Impact of spectral quality on toxicity of iron in Anabaena doliolum and Chlorella vulgaris.
Evidence is presented on the effects of low and high concentrations of iron on growth, nutrient uptake (NH+4 and NO3-), photosynthesis (CO2-fixation and O2-evolution) and nitrate reductase (NR) activity of A. doliolum and C. vulgaris under monochromatic irradiation. Control cultures (not treated with FeCl3) showed maximum growth under fluorescent followed by red, yellow, blue and green lights (fluorescent greater than yellow greater than red greater than blue greater than green). The inhibition was of synergistic type under yellow and red lights at all the iron concentrations tested. However, under blue and green lights the interaction was less than additive type. All the processes studied responded in a similar manner to a particular color of light. Under fluorescent light at low Fe concentrations, stimulation of NR, 14CO2-fixation and O2-evolution was noticed in both the test organisms. However, even the lowest concentration of iron tested was inhibitory to these processes under yellow and red lights. Under blue light at 20 micrograms.ml-1 Fe, NR activity was inhibited by 98%. This study clearly demonstrates that metal toxicity to phytoplankton will be greatly affected by spectral quality, hence it will have great significance in limnological research.
Mallick
N
N
Laboratory of Algal Biology, Banaras Hindu University, Varanasi, India.
Rai
L C
LC
D017033:Anabaena / Q000187:drug effects* / Q000254:growth & development; D000704:Analysis of Variance; D002708:Chlorella / Q000187:drug effects* / Q000254:growth & development; D007501:Iron / Q000633:toxicity*; D008027:Light; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis
D007501:Iron; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D010100:Oxygen