Methylated cytosine level in human liver DNA does not decline in aging process.
In order to ascertain a generality of the age-dependent decrease in DNA methylation level among different mammalian species, methylated cytosine contents in human liver and spleen DNA at different ages have been determined using high performance liquid chromatography (HPLC). Unexpectedly, the liver DNA revealed no appreciable decline with age while the spleen DNA showed a slight reduction. It indicates that a decrease of methylation level in genomic DNA is not a common denominator of age-related changes in mammals.
Tawa
R
R
Department of Analytical Chemistry, Kyoto Pharmaceutical University, Japan.
Ueno
S
S
Yamamoto
K
K
Yamamoto
Y
Y
Sagisaka
K
K
Katakura
R
R
Kayama
T
T
Yoshimoto
T
T
Sakurai
H
H
Ono
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D000818:Animals; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000378:metabolism*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008099:Liver / Q000378:metabolism; D008297:Male; D008745:Methylation; D051379:Mice; D008875:Middle Aged; D051381:Rats; D013045:Species Specificity; D013154:Spleen / Q000378:metabolism
D003841:Deoxycytidine; D004247:DNA; C016569:5-methyldeoxycytidine