Economic considerations important in the scale-up of an ovalbumin separation from hen egg-white on the anion-exchange cellulose DE92.
The scale-up of the separation of hen egg-white proteins has been investigated using Whatman DE92 anion-exchange cellulose. Having developed suitable chromatographic conditions, a maximum binding capacity of 100 mg protein/ml packed DE92 was determined in a 25-ml column. The process was scaled up 1000-fold and the influence of batch and column techniques on the chromatographic step assessed. Data indicate column processes to be more efficient than batch in the adsorptive stage.
Levison
P R
PR
Whatman Specialty Products Division, Springfield Mill, Maidstone, Kent, UK.
Badger
S E
SE
Toome
D W
DW
Koscielny
M L
ML
Lane
L
L
Butts
E T
ET
D000818:Animals; D002645:Chickens; D002852:Chromatography, Ion Exchange / Q000191:economics*; D004529:Egg White; D005260:Female; D010047:Ovalbumin / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D010047:Ovalbumin