title: Isolation of a cDNA clone encoding the RNase-superfamily-related gene highly expressed in chicken bone marrow cells.
abstract: The RNase gene superfamily combines functionally divergent proteins which share statistically significant sequence similarity. Known members assigned to this family include secretory and nonsecretory RNases; angiogenin; eosinophil cationic protein; eosinophil-derived neurotoxin; sialic-acid binding lectin and anti-tumor protein P-30. We report the cDNA cloning of the chicken RNase Super Family Related (RSFR) gene that is specifically overexpressed in normal bone marrow cells and bone marrow-derived AMV transformed monoblasts. It codes for a 139 amino acid protein with a putative signal peptide and remarkable conservation of active-site residues, other residues known to be important for substrate binding and catalytic activity and half-cystine residues common for all RNase family members. Phylogenetic tree analysis shows that RSFR defines a new group of genes within the family. We also conclude that an amino acid sequence block CKXXNTF(X) 11C is a "shortest RNase superfamily signature" which is both necessary and sufficient to identify all previously recognized family members as well as chicken RSFR.
authors.affiliation: Institute of Carcinogenesis: All-Union Cancer Research Center, Moscow, Russia.
authors.forename: E M
authors.initials: EM
authors.lastname: Klenova
authors.forename: I
authors.initials: I
authors.lastname: Botezato
authors.forename: V
authors.initials: V
authors.lastname: Laudet
authors.forename: G H
authors.initials: GH
authors.lastname: Goodwin
authors.forename: J C
authors.initials: JC
authors.lastname: Wallace
authors.forename: V V
authors.initials: VV
authors.lastname: Lobanenkov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000201:enzymology*; D002460:Cell Line; D002645:Chickens; D004277:DNA, Single-Stranded / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D010802:Phylogeny; D012260:Ribonucleases / Q000096:biosynthesis / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
chemical_list: D004277:DNA, Single-Stranded; D012260:Ribonucleases