Non-enzymatic glycation of apolipoprotein B in the sera of diabetic and non-diabetic subjects.
Utilising a combination of m-aminophenyl-borate affinity chromatography and an immunoradiometric assay for apolipoprotein B (apo B), we have developed a specific and highly sensitive (6 ng/ml) procedure for the assay of glycated apo B. We studied 52 diabetic patients, 50 non-diabetic control subjects and 12 patients heterozygous for familial hypercholesterolaemia (FH). Both insulin-dependent and non-insulin dependent diabetics were included in our study. Total apo B in the diabetics (108 +/- 5 mg/dl; mean +/- S.E.M) was increased (controls: 95 +/- 4 mg/dl; P less than 0.05). In the FH group the serum apo B concentration (216 +/- 24 mg/dl) was significantly higher (P less than 0.001) than both the other groups studied. Both the serum glycated apo B concentration (9.3 +/- 0.8 mg/dl versus 4.8 +/- 0.7 mg/dl) and the percentage glycated apo B (7.9 +/- 0.4% compared to 3.9 +/- 0.2%) were significantly higher in the diabetics than in non-diabetic controls (P less than 0.001). A positive correlation was found between the percentage of glycated apo B and glycated haemoglobin (r = 0.65; P less than 0.001) and fasting glucose concentration (r = 0.52; P less than 0.001) in diabetics. The percentage of glycated apo B in FH patients was not significantly different from controls, but the serum concentration of glycated apo B, because of the greatly increased total level of apo B was raised (8.2 +/- 1.4 mg/dl) to a similar extent to that of the diabetics.
Tames
F J
FJ
Department of Clinical Biochemistry, Manchester Royal Infirmary, UK.
Mackness
M I
MI
Arrol
S
S
Laing
I
I
Durrington
P N
PN
D000328:Adult; D000368:Aged; D001055:Apolipoproteins B / Q000097:blood*; D002846:Chromatography, Affinity; D003920:Diabetes Mellitus / Q000097:blood*; D005260:Female; D006031:Glycosylation; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000097:blood; D015592:Immunoradiometric Assay; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
D001055:Apolipoproteins B