Production and characterization of two monoclonal antibodies directed against the integrin beta 1 chain.
The production and characterization of two new monoclonal antibodies (MAbs), designated MAR4 and MAR5, raised against the partially purified alpha 5 beta 1 integrin, are described. The reactivity of these 2 MAbs on tumor cell lines indicated that they reacted on all the cells expressing the beta 1 subunit independently of the alpha 5 expression. Both MAbs were found to immunoprecipitate on 3 cell lines, a protein of 120 KD corresponding to the molecular weight be the beta 1 chain, in addition to proteins of other MW corresponding to the alpha subunits differentially expressed by these cells. The cross-competition experiments showed that MAR4 and MAR5 recognize the same epitope. These 2 MAbs seem to be useful reagents for the characterization of the VLA expression in tumor cells.
Pellegrini
R
R
Division of Experimental Oncology E, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
Bazzini
P
P
Tosi
E
E
Tagliabue
E
E
Conforti
G
G
Dejana
E
E
Ménard
S
S
Colnaghi
M I
MI
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D001667:Binding, Competitive; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D016023:Integrins / Q000276:immunology*; D007457:Iodine Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D016023:Integrins; D007457:Iodine Radioisotopes