Expression of HLA class-I antigens on renal cell carcinoma and non-transformed renal tissue.
To determine the HLA class-I antigen expression in renal cell carcinoma, the expression of beta-2-microglobulin (beta 2-m) was analyzed. 20 renal tumor specimens and 20 normal renal tissues were examined for beta 2-m expression by immunofluorescence. Homogenous staining of all normal renal tissues was observed, as expected. From the panel of 20 tumors, 17 also had a strong expression of beta 2-m. Furthermore, two newly established renal cancer cell lines exhibited a normal distribution of beta 2-m at two different passages. From these results we conclude that in most cases malignant transformation in renal cancer is not associated with a loss of HLA class-I antigens. This could be a favorable explanation for the restricted results of interferon-gamma in renal cell carcinoma, the main function of which is probably to enhance major histocompatibility complex class-I expression.
Buszello
H
H
Department of Urology, Heinrich Heine University of Düsseldorf, FRG.
Ackermann
R
R
D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D007668:Kidney / Q000473:pathology; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D001613:beta 2-Microglobulin / Q000235:genetics*
D006680:HLA Antigens; D001613:beta 2-Microglobulin