The possible role of ADP ribosylation in physiological regulation of sporulation in Streptomyces griseus.
The role of ADP ribosylation of proteins in the physiological regulation of sporulation in Streptomyces griseus was studied. We report here that both the activity of NAD+: arginine ADP-ribosyltransferase (ADPRT) and the pattern of ADP-ribosylated proteins showed characteristic changes during the life cycle in S. griseus 2682. Analysis off ADP-ribosylated proteins revealed that in a nonsporulating mutant of the parental wild-type (wt) strain (Bld7 mutant), both the activity of ADPRT and the pattern of ADP-ribosylated proteins were different from those of the parental strain. Addition of 3-aminobenzamide (3AB), the most potent inhibitor of ADPRT, inhibited sporulation of S. griseus 2682 and the A-factor (AF)-induced sporulation of S. griseus Bld7, but in both cases the inhibitory effect of 3AB was strictly age-dependent. Using [alpha-32P]GTP, we have demonstrated the presence of GTP-binding proteins in purified cell membranes of S. griseus 2682 and S. griseus Bld7. The same GTP-binding proteins were observed in Bld7 and the wt. AF stimulated the basal GTPase activity of cell membranes of S. griseus 2682 in a concentration-dependent manner, suggesting that GTP-binding proteins might be involved in the AF-induced sporulation process.
Penyige
A
A
Department of Biology, University Medical School, Debrecen, Hungary.
Vargha
G
G
Ensign
J C
JC
Barabás
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G
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D001549:Benzamides / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism; D007700:Kinetics; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology; D013305:Streptomyces griseus / Q000201:enzymology / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D000246:Adenosine Diphosphate Ribose; D000244:Adenosine Diphosphate; C025160:3-aminobenzamide; D011065:Poly(ADP-ribose) Polymerases; D019204:GTP-Binding Proteins