title: Cloning and overproduction of biodegradative threonine deaminase from Escherichia coli W strain.
abstract: We have cloned the structural gene (tdcB) of biodegradative threonine deaminase from Escherichia coli W strain by utilizing the polymerase chain reaction. The JM109/pUCTDA strain, which was obtained by transforming E. coli JM109 with a vector plasmid (pUCTDA) containing the cloned tdcB gene, produced a large amount of the enzyme corresponding to more than 5% of the total soluble protein. Amino acid sequence analysis of this recombinant enzyme showed that the amino acid sequence is identical to the nucleotide-deduced sequence of biodegradative threonine deaminase from E. coli K-12.
authors.affiliation: Department of Chemistry, Faculty of Science, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: M
authors.initials: M
authors.lastname: Fujita
authors.forename: M
authors.initials: M
authors.lastname: Takeuchi
authors.forename: N
authors.initials: N
authors.lastname: Yumoto
authors.forename: M
authors.initials: M
authors.lastname: Tokushige
authors.forename: Y
authors.initials: Y
authors.lastname: Kawata
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013913:Threonine Dehydratase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
chemical_list: D013913:Threonine Dehydratase