Enzyme immunoassay method for total alpha gonadotropin in human urine samples.
OBJECTIVE
To develop and validate an assay for urinary luteinizing hormone (LH) metabolite that can be used for frozen-thawed urine.


METHODS
The study is in two parts: (1) selected urine samples were evaluated to determine all components of urinary LH metabolism, and then (2) 26 complete menstrual cycles were evaluated using daily matched blood and urine samples.


METHODS
Urinary and serum samples were collected for normal women undergoing artificial insemination because of the infertility of their partners.


METHODS
Patients were selected to have normal ovarian function.


METHODS
None.


RESULTS
Serum LH and urinary LH metabolites were measured in daily samples from 25 ovulatory cycles.


RESULTS
The midcycle serum LH peak was highly correlated to the measurement of urinary LH metabolites in frozen:thawed urine samples.


CONCLUSIONS
A simple enzyme assay for LH/LH-alpha can be used to detect the midcycle LH surge on frozen-thawed urine samples. This method has practical application in a situation that involves self-collected urine samples stored frozen and later analyzed to determine the day of ovulation.
Clough
K M
KM
Hygeia Sciences, Inc., Newton, Massachusetts.
Cole
F X
FX
Seaver
S S
SS
Vesprini
A
A
Kuo
A Y
AY
Lasley
B L
BL
D000328:Adult; D003404:Creatinine / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005615:Freezing; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood / Q000652:urine*; D008597:Menstrual Cycle; D009994:Osmolar Concentration
D007986:Luteinizing Hormone; D003404:Creatinine