Regulation of acetylcholine release in vivo from rat hippocampus by monoamines as revealed by novel column-switching HPLC with electrochemical detection.
To clarify monoaminergic regulation of acetylcholine (ACh) release in the rat hippocampus, the effects of administration of monoamines through a dialysis probe on extracellular ACh levels were examined using in vivo brain microdialysis combined with a novel column-switching HPLC system. Infusion of dopamine or 5-hydroxytryptamine, but not noradrenaline, increased ACh levels. The ACh levels also increased following infusion of apomorphine and 5-methoxy-N,N-dimethyltryptamine. These results demonstrate that hippocampal ACh release is regulated by dopamine and 5-hydroxytryptamine.
Ohue
T
T
Department of Pharmacology, Kyoto University Faculty of Medicine, Japan.
Koshimura
K
K
Akiyama
Y
Y
Ito
A
A
Kido
T
T
Takagi
Y
Y
Miwa
S
S
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D015306:Biogenic Monoamines / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D004298:Dopamine / Q000502:physiology; D004563:Electrochemistry; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000502:physiology
D015306:Biogenic Monoamines; D012701:Serotonin; D000109:Acetylcholine; D004298:Dopamine; D009638:Norepinephrine