[Effect of carbon tetrachloride intoxication on leakage of intracellular potassium in regenerating rat hepatocytes in vitro].
Leakage of intracellular potassium and GPT in normal rat hepatocytes exposed to 10, 15 and 20 mmol/L of carbon tetrachloride (CCl4) in vitro was determined at 5, 10, 15 and 20 min after cell intoxication. Both of the intracellular potassium and GPT were clearly dose- and time-dependent with CCl4, but the leakage of intracellular potassium is a more sensitive index to indicate cell injury than the later. The percent change of intracellular potassium in regenerating rat hepatocytes 20 min after CCl4 (15 mmol/L) intoxication was markedly reduced than that in normal rat. This finding indicates that regenerating hepatocytes are resistant to CCl4 hepatotoxicity in vitro, and its mechanism presumably is due to a higher stability of regenerating liver cell membrane.
Zhang
B H
BH
Department of Physiology, Dalian Medical College.
Mei
M H
MH
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism* / Q000503:physiopathology; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism* / Q000503:physiopathology; D006498:Hepatectomy; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000410:Alanine Transaminase; D011188:Potassium