Investigation of the between-gel and within-gel variation in fragment size determinations found when using single locus DNA probes.
Measurement variation in the sizing of DNA fragments has been assessed, examining within-gel and between-gel variability. Also, measurement variation detected between two different laboratories, using both manual and automated measurement techniques, has been investigated and discussed.
Syndercombe Court
D
D
Department of Haematology, London Hospital Medical College, UK.
Fedor
T
T
Gouldstone
M
M
Lincoln
P J
PJ
Phillips
C P
CP
Tate
V
V
Thomson
J A
JA
Watts
P W
PW
D000704:Analysis of Variance; D001345:Autoradiography; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D009035:Mothers; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D015203:Reproducibility of Results
D015342:DNA Probes; D004247:DNA