title: Cloning and expression in yeast of a plant potassium ion transport system.
abstract: A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+ uptake with a complementary DNA library from Arabidopsis thaliana. A 2.65-kilobase complementary DNA conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or 86Rb+) at rates similar to those in wild-type yeast. The predicted amino acid sequence (838 amino acids) has three domains: a channel-forming region homologous to animal K+ channels, a cyclic nucleotide-binding site, and an ankyrin-like region.
authors.affiliation: Biochimie et Physiologie Végétales, ENSA-M/INRA/CNRS URA 573, Montpellier, France.
authors.forename: H
authors.initials: H
authors.lastname: Sentenac
authors.forename: N
authors.initials: N
authors.lastname: Bonneaud
authors.forename: M
authors.initials: M
authors.lastname: Minet
authors.forename: F
authors.initials: F
authors.lastname: Lacroute
authors.forename: J M
authors.initials: JM
authors.lastname: Salmon
authors.forename: F
authors.initials: F
authors.lastname: Gaymard
authors.forename: C
authors.initials: C
authors.lastname: Grignon
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001692:Biological Transport; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D015870:Gene Expression; D007700:Kinetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D010940:Plant Proteins; D015221:Potassium Channels; C074500:AKT1 protein, Arabidopsis; D004247:DNA; D015246:Deoxyribonuclease EcoRI; D011188:Potassium