Identification of a prenylation site in delta virus large antigen.
During replication, hepatitis delta virus (HDV) switches from production of small to large delta antigen. Both antigen isoforms have an HDV genome binding domain and are packaged into hepatitis B virus (HBV)-derived envelopes but differ at their carboxy termini. The large antigen was shown to contain a terminal CXXX box and undergo prenylation. The large, but not the small, antigen formed secreted particles when expressed singly with HBV surface antigen. Mutation of Cys211 in the CXXX box of the large antigen abolished both prenylation and particle formation, suggesting that this site is important for virion morphogenesis.
Glenn
J S
JS
Department of Pharmacology, University of California, San Francisco 94143-0450.
Watson
J A
JA
Havel
C M
CM
White
J M
JM
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002460:Cell Line; D003545:Cysteine; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D035923:Hepatitis delta Antigens; D008798:Mevalonic Acid / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012694:Serine; D014162:Transfection; D014779:Virus Replication
D000956:Antigens, Viral; D035923:Hepatitis delta Antigens; D012333:RNA, Messenger; C442244:hepatitis delta virus large antigen; D012694:Serine; D011392:Proline; D003545:Cysteine; D008798:Mevalonic Acid