title: The interaction of Cryptococcus neoformans with primary rat lung cell cultures.
abstract: Conditions under which Cryptococcus neoformans adhered to and was internalized by primary lung epithelial cell cultures were studied. Adherence was affected by the yeast culture age, glucose concentration and growth temperature. Formalin or heat treatment did not affect yeast adherence. Trypsin treatment, however, inhibited adherence.
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, Ft. Wayne 46805.
authors.forename: G J
authors.initials: GJ
authors.lastname: Merkel
authors.forename: R K
authors.initials: RK
authors.lastname: Cunningham
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003455:Cryptococcus neoformans / Q000378:metabolism* / Q000648:ultrastructure; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000382:microbiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D014357:Trypsin; D005947:Glucose