[Stabilization and protection of an enzymatic system, participating in the transformation of beta-carotene into retinal, during its isolation].
The authors studied the effect of dithiothreitol (DTT), carotenoids and protease inhibitors on stabilization and protection of the enzyme catalysing the conversion of beta-carotene into retinal during the enzyme isolation from the rabbit small intestine. The addition of 1 mM DTT into the homogenization mixture increased the activity of the enzyme 5 times compared with control. The additional introduction of 0.7 mg/ml soybean trypsin inhibitor or 2.10(-4) M phenylmethylsulfonyl fluoride increased the enzyme activity 2.1 and 1.2 times, respectively. Lutein, beta-carotene and lycopene at a concentration of 10 mg/ml increased the enzyme activity 2.1, 1.9 and 1.6 times respectively. The effects of DTT, lutein and the protease inhibitor depended on their concentrations and was of an independent additive character. The maximum activity of the isolated enzyme exceeded the control without DTT 15 times.
Dmitrovskiĭ
A A
AA
Ershov
Iu V
IuV
Bykhovskiĭ
V Ia
VIa
D000818:Animals; D002338:Carotenoids / Q000378:metabolism* / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D007421:Intestine, Small / Q000201:enzymology*; D010105:Oxygenases / Q000302:isolation & purification / Q000378:metabolism*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D011817:Rabbits; D012172:Retinaldehyde / Q000378:metabolism*; D019207:beta Carotene; D050559:beta-Carotene 15,15'-Monooxygenase
D011480:Protease Inhibitors; D019207:beta Carotene; D002338:Carotenoids; D010664:Phenylmethylsulfonyl Fluoride; D010105:Oxygenases; D050559:beta-Carotene 15,15'-Monooxygenase; D012172:Retinaldehyde; D004229:Dithiothreitol
Stabilizatsiia i zashchita fermentnoĭ sistemy, uchastvuiushcheĭ v orevrasgcgebuu v beta-karotina v retinal', pri ee vydelenii.