Inhibitory effect of topical application of a green tea polyphenol fraction on tumor initiation and promotion in mouse skin.
A green tea polyphenol fraction was evaluated for its ability to inhibit tumor initiation by polycyclic aromatic hydrocarbons and tumor promotion by a phorbol ester in the skin of CD-1 mice. Topical application of the green tea polyphenol fraction inhibited benzo[a]pyrene- and 7,12-dimethylbenz[a]-anthracene-induced tumor initiation as well as 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion. Topical application of the green tea polyphenol fraction also inhibited TPA-induced inflammation, ornithine decarboxylase activity, hyperplasia and hydrogen peroxide formation. Studies with individual polyphenolic compounds in green tea indicated that topical application of (-)-epigallocatechin gallate, (-)-epigallocatechin and (-)-epicatechin gallate inhibited TPA-induced inflammation in mouse epidermis.
Huang
M T
MT
Department of Chemical Biology and Pharmacognosy, College of Pharmacy, Rutgers, State University of New Jersey, Piscataway 08855-0789.
Ho
C T
CT
Wang
Z Y
ZY
Ferraro
T
T
Finnegan-Olive
T
T
Lou
Y R
YR
Mitchell
J M
JM
Laskin
J D
JD
Newmark
H
H
Yang
C S
CS
D015127:9,10-Dimethyl-1,2-benzanthracene; D000287:Administration, Topical; D000818:Animals; D001564:Benzo(a)pyrene; D004487:Edema / Q000139:chemically induced* / Q000517:prevention & control; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000378:metabolism*; D006965:Hyperplasia / Q000139:chemically induced; D051379:Mice; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D010636:Phenols / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control; D013662:Tea / Q000737:chemistry*; D013755:Tetradecanoylphorbol Acetate
D010636:Phenols; D013662:Tea; D001564:Benzo(a)pyrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D006861:Hydrogen Peroxide; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate