Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Legionella pneumophila.
An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismutases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.
Steinman
H M
HM
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461.
D001483:Base Sequence; D015139:Blotting, Southern; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D007527:Isoenzymes / Q000235:genetics*; D016952:Legionella pneumophila / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010269:Paraquat / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000235:genetics*
D004269:DNA, Bacterial; D007527:Isoenzymes; D015345:Oligonucleotide Probes; D013482:Superoxide Dismutase; D010269:Paraquat