Mapping and sequencing of an actively transcribed Euglena gracilis chloroplast gene (ccsA) homologous to the Arabidopsis thaliana nuclear gene cs(ch-42).
We mapped and sequenced a novel chloroplast gene encoding a protein (348 amino acids) which shows a high sequence identity with both the decoded nuclear cs(ch-42) gene product of Arabidopsis thaliana, and the C-terminal half of the decoded 'crtA' gene product of Rhodobacter capsulatus. The chloroplast gene (ccsA) is split (two exons) and transcribed into a stable mRNA of about 1200 nucleotides. The putative protein may be involved in the biosynthesis of photosynthetic pigments.
Orsat
B
B
Laboratoire de Biochimie végétale, Université de Neuchâtel, Switzerland.
Monfort
A
A
Chatellard
P
P
Stutz
E
E
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002736:Chloroplasts / Q000378:metabolism*; D005056:Euglena gracilis / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D009687:Nuclear Proteins; D015800:Protozoan Proteins; C075103:ccsA protein, protozoan