The DNA polymerase gene from chlorella viruses PBCV-1 and NY-2A contains an intron with nuclear splicing sequences.
The deduced amino acid sequences of two eukaryotic chlorella virus (PBCV-1 and NY-2A) DNA polymerases are 90% identical and contain amino acid motifs typical of alpha-like (Family B) DNA polymerases. The open reading frames of both PBCV-1 and NY-2A DNA polymerases are interrupted by an identically located, small (101 or 86 nucleotides, respectively) intron that resembles eukaryotic nuclear-spliced messenger RNA introns. This discovery suggests that chlorella virus replication has a nuclear phase.
Grabherr
R
R
Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
Strasser
P
P
Van Etten
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002708:Chlorella; D003062:Codon; D004267:DNA Viruses / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D015870:Gene Expression; D005814:Genes, Viral; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D015678:Viral Structural Proteins / Q000235:genetics*
D003062:Codon; D004279:DNA, Viral; D012333:RNA, Messenger; D015678:Viral Structural Proteins; D004259:DNA-Directed DNA Polymerase