Mapping and characterization of a novel human myc-like (MYCLK1) sequence.
The myc family of proto-oncogenes consists of several members that possess regions of sequence homology and some have known similarities in structure and function. We have isolated an 8.8-kb EcoRI fragment from a human genomic library by hybridization to a 28-base oligonucleotide probe derived from a region of the second exon of MYC, which is highly conserved in the myc gene family. Sequence analysis of this myc-like (MYCLK1) DNA fragment has revealed the existence of a region with 85% homology to the 28-base oligonucleotide probe. An open reading frame of 207 nucleotides containing the region of homology was found. We have mapped MYCLK1 to human chromosome 7 at band p15 by chromosome in situ hybridization; this site is distinct from the map location of previously characterized myc genes. Whether MYCLK1 represents a new functional member of the myc family of proto-oncogenes remains to be determined.
Robertson
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Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Morton
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CC
D001483:Base Sequence; D002897:Chromosomes, Human, Pair 7; D016259:Genes, myc; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D012689:Sequence Homology, Nucleic Acid