Isolation of different high-Mr mucin species from human whole saliva.
By using CsCl-density-gradient ultracentrifugation, two high-Mr mucin species were isolated from human whole saliva, having buoyant densities in 0.2 M-guanidinium chloride of approx. 1.56 g/ml (pool IA) and 1.48 g/ml (pool IIA). Analytical density-gradient centrifugation of submandibular, sublingual, labial and palatal saliva, followed by immunochemical analysis with anti-mucin monoclonal antibodies, indicated immunochemical and physicochemical similarities between the high-density mucins of pool IA and mucins from palatal salivary glands. Chemical analysis indicated that the putative palatal mucin was rich in sulphate, but poor in sialic acid. The lower-density mucins of pool IIA equated with the high-Mr mucins of submandibular-sublingual saliva, both immunochemically and physicochemically (buoyant density).
Veerman
E C
EC
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit, The Netherlands.
van den Keybus
P A
PA
Valentijn-Benz
M
M
Nieuw Amerongen
A V
AV
D000911:Antibodies, Monoclonal; D002241:Carbohydrates / Q000032:analysis; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004797:Enzyme-Linked Immunosorbent Assay; D019791:Guanidine; D006146:Guanidines; D006801:Humans; D009077:Mucins / Q000737:chemistry / Q000302:isolation & purification*; D019158:N-Acetylneuraminic Acid; D010159:Palate / Q000378:metabolism; D012463:Saliva / Q000737:chemistry*; D012794:Sialic Acids / Q000032:analysis; D013431:Sulfates / Q000032:analysis
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D006146:Guanidines; D009077:Mucins; D012794:Sialic Acids; D013431:Sulfates; D019158:N-Acetylneuraminic Acid; D019791:Guanidine