title: Conditions for protoplasting, regenerating, and transforming the calicheamicin producer, Micromonospora echinospora.
abstract: We report methods to generate protoplasts, to regenerate mycelia, and to transform Micromonospora echinospora. This actinomycete produces the unusual antitumor antibiotics, the calicheamicins. These protocols may be applied to other actinomycetes that have been difficult to transform. These methods also may facilitate the cloning of calicheamicin biosynthetic genes by genetic complementation.
authors.affiliation: Department of Microbial Genetics, Lederle Laboratories, Pearl River, New York 10965.
authors.forename: S F
authors.initials: SF
authors.lastname: Love
authors.forename: W M
authors.initials: WM
authors.lastname: Maiese
authors.forename: D M
authors.initials: DM
authors.lastname: Rothstein
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis; D053281:Enediynes; D007613:Kanamycin Resistance / Q000235:genetics; D008847:Micromonospora / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010957:Plasmids; D011523:Protoplasts; D014169:Transformation, Bacterial
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; C055955:calicheamicin gamma(1)I