An agglutinin with unique specificity for N-glycolyl sialic acid residues of thyroglobulin in the hemolymph of a marine crab Scylla serrata (Forskal).
A novel agglutinin with specificity for sialic acid sequence of sugars in thyroglobulin is identified in the hemolymph of Scylla serrata. The physico-chemical characteristics of its binding affinity, such as pH and temperature optima, and cationic requirements are defined. N-glycolyl neuraminic acid (NeuGc) (at 0.6 mM), in contrast to N-acetyl neuraminic acid (NeuAc) (at greater than 5.0 mM), is the potent inhibitor of hemagglutination. Bovine and porcine thyroglobulins containing NeuGc, inhibited the agglutination. NeuGc-acid glycoprotein fraction (bovine) but not NeuAc-acid glycoprotein fraction (human) inhibited the hemagglutination. The inability of other NeuGc-glycoproteins (bovine submaxillary mucin) to inhibit the agglutination suggests that the agglutinin may also recognize glycosidic linkage associated with NeuGc. The potential of the agglutinin in identifying NeuGc containing human tumor associated antigens is discussed.
Mercy
P D
PD
Department of Zoology, Holy Cross College, Tamilnadu, India.
Ravindranath
M H
MH
D000327:Adsorption; D000818:Animals; D003386:Brachyura / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004355:Drug Stability; D006168:Guinea Pigs; D006384:Hemagglutination / Q000187:drug effects; D006388:Hemagglutinins / Q000737:chemistry / Q000378:metabolism*; D006458:Hemolymph / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D019158:N-Acetylneuraminic Acid; D009438:Neuraminic Acids / Q000378:metabolism / Q000494:pharmacology; D011817:Rabbits; D012794:Sialic Acids / Q000378:metabolism*; D013954:Thyroglobulin / Q000378:metabolism*
D006388:Hemagglutinins; D009438:Neuraminic Acids; D012794:Sialic Acids; C032592:N-glycolylneuraminic acid; D013954:Thyroglobulin; D019158:N-Acetylneuraminic Acid