Endocytosis of different members of the small chondroitin/dermatan sulfate proteoglycan family.
The family of small interstitial chondroitin/dermatan sulfate proteoglycans consists of at least three different molecular species: biglycan (proteoglycan I), decorin (proteoglycan II), and proteoglycan-100, which has a glycosylated core protein of about 100 kDa. The core protein of decorin has been shown to be responsible for receptor-mediated endocytosis of this proteoglycan species by a variety of mesenchymal cells. It is now demonstrated that skin fibroblasts and articular chondrocytes endocytose biglycan with an efficiency similar to that of decorin. Uptake of biglycan is also mediated by its core protein and can be inhibited by decorin in a partially competitive manner. In human fibroblasts, endosomal proteins of 51 and 26 kDa, which are known to bind decorin core protein, also interact with biglycan. This interaction can be inhibited by decorin. Bovine articular chondrocytes contained binding proteins of 48 and 25 kDa. Proteoglycan-100 can be distinguished from biglycan and decorin by its low clearance rate, which however, exceeds the rate of fluid phase endocytosis.
Hausser
H
H
Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Germany.
Ober
B
B
Quentin-Hoffmann
E
E
Schmidt
B
B
Kresse
H
H
D015153:Blotting, Western; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism*; D003871:Dermatan Sulfate / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004705:Endocytosis; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism; D011233:Precipitin Tests; D012867:Skin / Q000166:cytology / Q000378:metabolism; D014407:Tumor Cells, Cultured
D011508:Chondroitin Sulfate Proteoglycans; C032649:dermatan sulfate proteoglycan; D003871:Dermatan Sulfate