Selective extracellular release of cholera toxin B subunit by Escherichia coli: dissection of Neisseria Iga beta-mediated outer membrane transport.
The C-terminal domain (Iga beta) of the Neisseria IgA protease precursor is involved in the transport of covalently attached proteins across the outer membrane of Gram-negative bacteria. We investigated outer membrane transport in Escherichia coli using fusion proteins consisting of an N-terminal signal sequence for inner membrane transport, the Vibrio cholerae toxin B subunit (CtxB) as a passenger and Iga beta. The process probably involves two distinct steps: (i) integration of Iga beta into the outer membrane and (ii) translocation of the passenger across the membrane. The outer membrane integrated part of Iga beta is the C-terminal 30 kDa core, which serves as a translocator for both the passenger and the linking region situated between the passenger and Iga beta core. The completeness of the translocation is demonstrated by the extracellular release of the passenger protein owing to the action of the E. coli outer membrane OmpT protease. Translocation of the CtxB moiety occurs efficiently under conditions preventing intramolecular disulphide bond formation. In contrast, if disulphide bond formation in the periplasm proceeds, then translocation halts after the export of the linking region. In this situation transmembrane intermediates are generated which give rise to characteristic fragments resulting from rapid proteolytic degradation of the periplasmically trapped portion. Based on the identification of translocation intermediates we propose that the polypeptide chain of the passenger passes in a linear fashion across the bacterial outer membrane.
Klauser
T
T
Max-Planck-Institut für Biologie, Abteilung Infektionsbiologie, Tübingen, FRG.
Pohlner
J
J
Meyer
T F
TF
D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D002772:Cholera Toxin / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004768:Enterotoxins / Q000235:genetics / Q000378:metabolism; D004792:Enzyme Precursors / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015151:Immunoblotting; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009343:Neisseria / Q000235:genetics* / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism*; D014734:Vibrio cholerae / Q000235:genetics
D004768:Enterotoxins; D004792:Enzyme Precursors; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; C037420:stN protein, Vibrio cholerae; D002772:Cholera Toxin; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase; C057840:omptin outer membrane protease