title: Mitogenic activity of high molecular polysaccharide fractions isolated from the cuppressaceae Thuja occidentalis L. enhanced cytokine-production by thyapolysaccharide, g-fraction (TPSg).
abstract: Thuja polysaccharide g fraction (TPSg) was shown to be an inducer of the CD4+ fraction of the human peripheral blood T-cell subset (1,2). Furthermore, it could be demonstrated that TPSg is a potent inhibitor of the expression of HIV-1-specific antigens and of the HIV-1-specific reverse transcriptase (3). This report deals with the cytokine pattern induced by TPSg in human peripheral blood lymphocyte (PBL) and purified monocyte/macrophage cultures. In addition, a further characterization of the CD4+ T-cell fraction stimulated by TPSg was performed by FACS analysis. TPSg is induces IL-1 beta, IL-2, IL-3, IL-6, gamma-IFN, G-CSF, GM-CSF, and TNF-beta production in PBL cultures; and IL-1 beta and IL-6 in monocyte/macrophage cultures. Enzyme-linked immunosorbent assays (ELISAs) demonstrated that no IL-4 was produced by PBL cultures under TPSg influence.
authors.affiliation: Medical Clinic, University Hospital, Eppendorf, Hamburg, FRG.
authors.forename: R
authors.initials: R
authors.lastname: Offergeld
authors.forename: C
authors.initials: C
authors.lastname: Reinecker
authors.forename: E
authors.initials: E
authors.lastname: Gumz
authors.forename: S
authors.initials: S
authors.lastname: Schrum
authors.forename: R
authors.initials: R
authors.lastname: Treiber
authors.forename: R D
authors.initials: RD
authors.lastname: Neth
authors.forename: S H
authors.initials: SH
authors.lastname: Gohla
mesh_terms: D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D029783:Thuja
chemical_list: D016207:Cytokines; D010936:Plant Extracts; D011134:Polysaccharides; C103413:Thuja polysaccharide g fraction