Glucose metabolism in proliferating epithelial cells from the rat colon.
1. The effects of fasting and fasting followed by refeeding on the activities of the oxidative pentose pathway (OPP) and the tricarboxylic acid cycle (TCA) in isolated rat colonocytes were estimated by the rate of production of 14CO2 from [1-14C]glucose and [6-14C]glucose, respectively. 2. Refeeding after a fast induced a 2-3-fold increase in glucose flux through the OPP and TCA cycle and the degree of change was similar in colonocytes from the proximal and distal colon. 3. Butyrate at a concentration of 40 mM inhibited the OPP by 20-30% (P less than 0.05) but had no effect on the activity of the TCA cycle. Glutamine at a concentration of 2 mM decreased the glucose flux through both the OPP and the TCA cycle by 30-50% (P less than 0.05). 4. Production of 14CO2 from the oxidation of butyrate or glucose indicated that the former was 5-7 times more active in colonocytes from fasted rats. After refeeding, however, butyrate utilization was similar to fasting values in the proximal colon but significantly lower (P less than 0.05) in the distal colon.
Butler
R N
RN
Department of Gastroenterology, Queen Elizabeth Hospital, Woodville, South Australia.
Goland
G
G
Jarrett
I G
IG
Fettman
M J
MJ
Roberts-Thomson
I C
IC
D000818:Animals; D002087:Butyrates / Q000378:metabolism; D020148:Butyric Acid; D002455:Cell Division; D003106:Colon / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D004435:Eating / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000502:physiology; D005215:Fasting / Q000502:physiology; D005260:Female; D005947:Glucose / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D002087:Butyrates; D020148:Butyric Acid; D005947:Glucose