Encoded combinatorial chemistry.
The diversity of chemical synthesis and the power of genetics are linked to provide a powerful, versatile method for drug screening. A process of alternating parallel combinatorial synthesis is used to encode individual members of a large library of chemicals with unique nucleotide sequences. After the chemical entity is bound to a target, the genetic tag can be amplified by replication and utilized for enrichment of the bound molecules by serial hybridization to a subset of the library. The nature of the chemical structure bound to the receptor is decoded by sequencing the nucleotide tag.
Brenner
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Department of Chemistry, Scripps Research Institute, La Jolla, CA 92037.
Lerner
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D000595:Amino Acid Sequence; D001483:Base Sequence; D016208:Databases, Factual; D015195:Drug Design; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping
D010455:Peptides