The use of a packed column for the determination of bupivacaine in human plasma by gas chromatography: an application in a pharmacokinetic study of bupivacaine.
The method involves a single extraction of the drug and the internal standard, etidocaine, from alkalinized plasma with n-hexane. The gas chromatograph is equipped with a glass column (2.0 m x 2 mm) packed with 3% W/W SP2250 on Chromosorb W, (80/100 mesh) and a nitrogen sensitive detector. The method can accurately measure plasma bupivacaine concentrations down to 0.01 micrograms ml-1 using a sample size of 0.5 ml. The day-to-day variation of bupivacaine at 2.0 micrograms ml-1 is 6.90% (n = 10). The calibration graph is linear over the range 0.1-4.0 micrograms ml-1. The method is accurate, fast and sensitive and has been applied in a pharmacokinetic study of bupivacaine.
Lau
O W
OW
Department of Chemistry, Chinese University of Hong Kong, Shatin.
Wong
Y C
YC
Chan
K
K
D000042:Absorption; D002045:Bupivacaine / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D002138:Calibration; D002849:Chromatography, Gas; D005041:Etidocaine / Q000032:analysis; D006207:Half-Life; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012015:Reference Standards; D014018:Tissue Distribution
D005041:Etidocaine; D002045:Bupivacaine