Identification of a ROB-1 beta-lactamase in Haemophilus ducreyi.
A collection of 100 clinical isolates of Haemophilus ducreyi from Thailand were all found to harbor a 5.4-kb plasmid, designated pTH126, which was shown to contain the bla ROB-1 gene. Restriction enzyme analysis and DNA-DNA hybridization studies confirmed that pTH126 was similar to the ROB-1 beta-lactamase plasmid pVM105 from Actinobacillus pleuropneumoniae. In approximately one-half of the isolates, pTH126 was found together with pHD131, which mediates TEM-1 beta-lactamase production.
Maclean
I W
IW
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
Slaney
L
L
Juteau
J M
JM
Levesque
R C
RC
Albritton
W L
WL
Ronald
A R
AR
D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D006191:Haemophilus ducreyi / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D007525:Isoelectric Focusing; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping; D013785:Thailand; D001618:beta-Lactamases / Q000032:analysis*
D004269:DNA, Bacterial; C071165:beta-lactamse ROB-1; D001618:beta-Lactamases