title: Gene expression in the Streptomyces temperate phage phi C31.
abstract: The repressor gene, c, of the temperate Streptomyces phage, phi C31 was previously cloned and sequenced, and predicted to encode a 74-kDa protein. The c gene actually produces three in-frame, N-terminally different, C-terminally identical proteins of 74, 54 and 42 kDa. The repressor proteins are translated from a corresponding nest of transcripts. Genetic and biochemical evidence suggests that the transcription of the c locus is autoregulated possibly by the 42-kDa protein binding to a highly conserved 16-bp perfect inverted repeat. The 16-bp sequence is present at at least twelve loci throughout the phi C31 genome. Transcription of the 'early' region is complex, possibly involving phage-specific promoters. The phi C31 terminators display sequence conservation and may be regulated. The phi C31 gene 'k' may encode a nucleotide kinase-encoding gene.
authors.affiliation: Department of Biological and Molecular Science, University of Stirling, Scotland, UK.
authors.forename: M C
authors.initials: MC
authors.lastname: Smith
authors.forename: C J
authors.initials: CJ
authors.lastname: Ingham
authors.forename: C E
authors.initials: CE
authors.lastname: Owen
authors.forename: N T
authors.initials: NT
authors.lastname: Wood
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012097:Repressor Proteins / Q000235:genetics* / Q000502:physiology; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D012097:Repressor Proteins