title: Use of colistin-polymyxin B-cellobiose agar for isolation of Vibrio vulnificus from the environment.
abstract: Colistin-polymyxin B-cellobiose agar was employed for the isolation of Vibrio vulnificus from shellfish. Isolates were examined phenotypically and with a gene probe and monoclonal antibody specific for V. vulnificus. Results indicated that colistin-polymyxin B-cellobiose agar is superior to both sodium dodecyl sulfate-polymyxin B-sucrose agar and thiosulfate-citrate-bile salts-sucrose agar in its ability to select and differentiate this species from background vibrios.
authors.affiliation: Department of Biology, University of North Carolina, Charlotte 28223.
authors.forename: J D
authors.initials: JD
authors.lastname: Oliver
authors.forename: K
authors.initials: K
authors.lastname: Guthrie
authors.forename: J
authors.initials: J
authors.lastname: Preyer
authors.forename: A
authors.initials: A
authors.lastname: Wright
authors.forename: L M
authors.initials: LM
authors.lastname: Simpson
authors.forename: R
authors.initials: R
authors.lastname: Siebeling
authors.forename: J G
authors.initials: JG
authors.lastname: Morris
mesh_terms: D000362:Agar; D001431:Bacteriological Techniques; D002475:Cellobiose; D003091:Colistin; D005069:Evaluation Studies as Topic; D010641:Phenotype; D011112:Polymyxin B; D012758:Shellfish / Q000382:microbiology*; D014733:Vibrio / Q000302:isolation & purification*
chemical_list: D002475:Cellobiose; D000362:Agar; D011112:Polymyxin B; D003091:Colistin