Renal platelet-derived growth factor gene expression in NZB/W F1 mice with lupus and ddY mice with IgA nephropathy.
The present study was carried out to determine how platelet-derived growth factor (PDGF)-A and -B chain mRNA expression correlate with disease activity in the renal cortex of NZB/W F1 mice, a model of systemic lupus erythematosus, and ddY mice, a model of IgA nephropathy. The PDGF-A and -B chain mRNA levels increased significantly as nephritis progressed in NZB/W F1 mice. In the renal cortex of ddY mice, however, the PDGF mRNA levels increased slightly with age. The PDGF mRNA levels in ddY mice were lower than those in NZB/W F1 mice. In the NZW control kidneys, the PDGF mRNA levels changed minimally throughout the experiments. A positive correlation was noted between PDGF mRNA levels and histopathological changes in renal tissues. At the onset of nephritis, NZB/W F1 mice were divided into two groups that received either methylprednisolone (MPSL) or saline injections for 5 months. The development of histopathological lesions and the increased PDGF mRNA levels were suppressed by MPSL treatment. These data suggest that PDGF plays a role in the progression of nephritis and that the beneficial effect of MPSL correlates with its ability to decrease the abnormally high PDGF mRNA levels seen in lupus nephritis.
Nakamura
T
T
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Ebihara
I
I
Nagaoka
I
I
Tomino
Y
Y
Koide
H
H
D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005922:Glomerulonephritis, IGA / Q000235:genetics*; D006417:Hematuria / Q000503:physiopathology; D007150:Immunohistochemistry; D007668:Kidney / Q000737:chemistry; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008775:Methylprednisolone / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D008814:Mice, Inbred NZB; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D015995:Prevalence; D011507:Proteinuria / Q000188:drug therapy / Q000453:epidemiology; D012333:RNA, Messenger / Q000032:analysis
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D008775:Methylprednisolone