title: Identification of a protein-binding surface by differential amide hydrogen-exchange measurements. Application to Bowman-Birk serine-protease inhibitor.
abstract: The binding surface of soybean trypsin/chymotrypsin Bowman-Birk inhibitor in contact with alpha-chymotrypsin has been identified by measurement of the change in amide hydrogen-exchange rates between free and chymotrypsin-bound inhibitor. Exchange measurements were made for the enzyme-bound form of the inhibitor at pH 7.3, 25 degrees C using fast-flow affinity chromatography and direct measurement of exchange rates in the protein complex from one-dimensional and two-dimensional nuclear magnetic resonance spectra. The interface is characterized by a broad surface of contact involving residues 39 through 48 of the anti-chymotryptic domain beta-hairpin as well as residues 32, 33 and 37 in the anti-chymotryptic domain loop of the inhibitor. A number of residues in the anti-tryptic domain of the protein also have an altered exchange rate, suggesting that there are changes in the protein conformation upon binding to chymotrypsin. These changes in amide exchange behavior are discussed in light of a model of the complex based on the X-ray crystallographic structure of turkey ovomucoid inhibitor third domain bound to a alpha-chymotrypsin, and the structure of free Bowman-Birk inhibitor determined in solution by two-dimensional nuclear magnetic resonance spectroscopy. The chymotrypsin-binding loop of Bowman-Birk inhibitor in the model is remarkably similar to the binding loop conformation in crystal structures of enzyme-bound polypeptide chymotrypsin inhibitor-I from potatoes, turkey ovomucoid inhibitor third domain, and chymotrypsin inhibitor-II from barley seeds.
authors.affiliation: Chemical Biodynamics Division, Lawrence Berkeley Laboratory, University of California 94720.
authors.forename: M H
authors.initials: MH
authors.lastname: Werner
authors.forename: D E
authors.initials: DE
authors.lastname: Wemmer
mesh_terms: D000577:Amides / Q000737:chemistry; D001665:Binding Sites; D002918:Chymotrypsin / Q000737:chemistry / Q000648:ultrastructure; D006860:Hydrogen Bonding; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D013057:Spectrum Analysis; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D000577:Amides; D046911:Macromolecular Substances; D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D002918:Chymotrypsin