title: Filipin labeling in Chlamydomonas gametes exposes site-specific lipid specializations.
abstract: A thin section study of mating Chlamydomonas cell wall-less CW 15 mating type plus (mt+) and mating type minus (mt-) gametes utilized filipin. The results show extensive labeling of mt+ and mt- plasma membranes. No labeling was seen on the mating structure membranes of activated mt+ or mt- gametes. These results indicate that differences exist between the plasma membrane and the mating structure membrane of gametes. If filipin is specific for the 3-beta-OH sterol, ergosterol and/or other Chlamydomonas sterols, then these results imply that the fusing mating structure membranes may be altered or reduced in sterol content. Such lipid specializations may increase local membrane fluidity and thereby facilitate the site-specific cell fusion associated with mating Chlamydomonas gametes.
authors.affiliation: Molecular Biology Institute, San Diego State University, CA 92182.
authors.forename: R L
authors.initials: RL
authors.lastname: Weiss Bizzoco
authors.forename: V
authors.initials: V
authors.lastname: Reyes
mesh_terms: D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000737:chemistry* / Q000502:physiology; D005372:Filipin; D008561:Membrane Fusion / Q000502:physiology; D008563:Membrane Lipids / Q000032:analysis*; D012098:Reproduction / Q000502:physiology; D013261:Sterols / Q000032:analysis*
chemical_list: D008563:Membrane Lipids; D013261:Sterols; D005372:Filipin