title: Cytokines and Ly-1 (B1) B cells.
abstract: In an attempt to elucidate the possible role of cytokines in autocrine growth of Ly-1+B cells, and the role of this subset of B cells in immune regulation, both in normal and diseased hosts, we have performed a systematic analysis of cytokine production by a series of mouse Ly-1+B lymphomas, as well as normal peritoneal Ly-1+ and conventional B cells. The lymphomas all express TGF-beta, and some express IL-3 and IL-4. We observed that both the lymphomas and the peritoneal cells produce TNF-alpha, TNF-beta and IL-6. Another cytokine, IL-10, is produced predominantly by peritoneal Ly-1+B cells from healthy mice and by Ly-1+ B lymphomas, but not by conventional B cells. As IL-10 regulates the production of monokines and a subset of T-cell derived cytokines, our results suggest a broad immunoregulatory role for Ly-1 B cells. To complement these studies we have also examined the responses of Ly-1 B cells to mitogens and cytokines previously shown to stimulate conventional B cells. In summary, Ly-1 B cells, in contrast to conventional B cells do not respond to anti-Ig antibodies, even in the presence of IL-4. They do respond to LPS, and this response is preferentially enhanced by IL-5, and marginally enhanced by IL-3. Surprisingly LPS-induced proliferation of peritoneal B cells is inhibited by IL-6 and to a greater extent by IL-10. Whether this inhibition is a result of differentiation into Ig secreting cells is currently being evaluated. We discuss our findings in terms of the potential of Ly-1 B cells to regulate their own development and the immunocompetence of other cells.
authors.affiliation: DNAX Research Institute, Palo Alto, CA 94304.
authors.forename: A
authors.initials: A
authors.lastname: O'Garra
authors.forename: M
authors.initials: M
authors.lastname: Howard
mesh_terms: D000818:Animals; D000950:Antigens, Ly; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D002454:Cell Differentiation; D016207:Cytokines / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice
chemical_list: D000950:Antigens, Ly; D016207:Cytokines; D016753:Interleukin-10