Heterogeneity of S-layer proteins of Lactobacillus acidophilus strains.
An S-layer (surface regular array) was found in the cell wall from six out of ten strains of Lactobacillus acidophilus examined by electron microscopic observations. All of the six strains which were shown to carry the S-layers belonged to the deoxyribonucleic acid (DNA) homology group A, but not to B, which had been classified by Johnson et al (Int. J. Syst. Bacteriol. 30: 53-68, 1980). On the other hand, the other four strains which possessed no S-layers were in the homology group B. The apparent molecular weights of the S-layer proteins ranged from 41 to 49 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Of the S-layer proteins from the six strains, three were susceptible to chemical cleavage with N-chlorosuccinimide, giving different peptide maps. All of the six S-layer proteins were fragmented by limited proteolysis with Staphylococcus aureus V8 protease, and gave markedly different peptide patterns by the subsequent peptide mapping analysis, except that the peptide maps of the S-layer proteins from the two strains which were in the same subgroup were identical.
Masuda
K
K
Department of Microbiology, Hyogo Prefectural Institute of Public Health, Japan.
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D007779:Lactobacillus acidophilus / Q000235:genetics* / Q000648:ultrastructure; D008970:Molecular Weight; D010446:Peptide Fragments; D010449:Peptide Mapping; D010641:Phenotype
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D010446:Peptide Fragments