Alphavirus spike-nucleocapsid interaction and network antibodies.
Vaux et al. (D. J. T. Vaux, A. Helenius, and I. Mellman, Nature (London) 336:36-42, 1988) recently reported the production of network antibodies that were suggested to have reconstructed a specific interaction between the nucleocapsid of Semliki Forest virus and the cytoplasmic tail of the viral E2 spike protein. The F13 anti-idiotype antibody, which was raised against anti-E2 tail antibodies, was claimed to recognize the virus nucleocapsid. In this report, we have used recombinant SFV viruses to demonstrate that the F13 antibody is not nucleocapsid specific but instead most likely recognizes some component of the viral replication machinery.
Suomalainen
M
M
Department of Molecular Biology, Karolinska Institute, Huddinge, Sweden.
Garoff
H
H
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002213:Capsid / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002872:Chromosome Deletion; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D016679:Genome, Viral; D006801:Humans; D008715:Methionine / Q000378:metabolism; D011485:Protein Binding; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism; D012672:Semliki forest virus / Q000235:genetics / Q000378:metabolism*; D014758:Viral Core Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000235:genetics
D000906:Antibodies; D000911:Antibodies, Monoclonal; D011990:Receptors, Transferrin; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; D008715:Methionine