Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro.
Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium chloride, on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes, delta 5-3 beta-HSD and 17 beta-HSD activity. Stage II ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting only nonesterified cholesterol moiety and stimulating the activity of both. Safe doses of above mentioned toxicants when added separately to stage II ovarian tissue with oLH (1 microgram/incubation) gonadotropin-induced depletion of nonesterified cholesterol and gonadotropin-induced stimulation of the activity of both enzymes was significantly inhibited. Esterified cholesterol remained almost unaltered. Findings clearly indicate the impairment of gonadotropin induced fish ovarian steroidogenesis by the four toxicants separately.
Mukherjee
D
D
Department of Zoology, University of Kalyani, India.
Guha
D
D
Kumar
V
V
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D002104:Cadmium / Q000009:adverse effects; D019256:Cadmium Chloride; D002347:Carps; D002712:Chlorides / Q000009:adverse effects; D002784:Cholesterol / Q000032:analysis*; D005260:Female; D006062:Gonadotropins / Q000502:physiology*; D008627:Mercuric Chloride / Q000009:adverse effects; D009097:Multienzyme Complexes / Q000378:metabolism*; D009865:Oocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010053:Ovary / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D019800:Phenol; D010636:Phenols / Q000009:adverse effects; D011375:Progesterone Reductase / Q000378:metabolism*; D013251:Steroid Isomerases / Q000378:metabolism*; D013440:Sulfides / Q000009:adverse effects; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
C021425:3 beta-hydroxysteroid oxidoreductase-delta(5) 3-ketosteroid isomerase; D002712:Chlorides; D006062:Gonadotropins; D009097:Multienzyme Complexes; D010636:Phenols; D013440:Sulfides; D014874:Water Pollutants, Chemical; D002104:Cadmium; D019800:Phenol; D008627:Mercuric Chloride; D002784:Cholesterol; D015067:17-Hydroxysteroid Dehydrogenases; D011375:Progesterone Reductase; D013251:Steroid Isomerases; D019256:Cadmium Chloride