title: Endothelial modulation of porcine coronary microcirculation perfused via immature collaterals.
abstract: Porcine hearts have relatively few native collateral vessels and lack the propensity to develop normal perfusion to the collateral-dependent myocardium. To examine microvascular responses in the collateral-dependent region, collateral vessels were stimulated in pigs by the Ameroid constrictor technique. After 4-7 wk, isolated microarterial vessels (90-170 microns ID) were studied in a pressurized (40 mmHg), no-flow state. Microvessels from noninstrumented pigs were used as controls for vascular studies. Although myocardium in the collateral-dependent region showed minimal evidence of infarction, percent systolic shortening was reduced at rest and after pacing compared with myocardium in the normally perfused region. Relaxations to the receptor-mediated endothelium-dependent agents ADP and bradykinin were impaired in collateral-dependent coronary microvessels. Relaxations to the calcium ionophore A23187, which acts through a non-receptor-mediated mechanism, were similar in control and Ameroid microvessels. Relaxations to the endothelium-independent agent sodium nitroprusside were markedly enhanced in microvessels from the collateral-dependent region compared with microvessels from control hearts. In conclusion, receptor-mediated endothelium-dependent relaxation is impaired and endothelium-independent relaxation to sodium nitroprusside is enhanced in microvessels from myocardium perfused by immature collateral vessels.
authors.affiliation: Department of Surgery, Beth Israel Hospital, Boston, Massachusetts 02215.
authors.forename: F W
authors.initials: FW
authors.lastname: Sellke
authors.forename: Y
authors.initials: Y
authors.lastname: Kagaya
authors.forename: R G
authors.initials: RG
authors.lastname: Johnson
authors.forename: T
authors.initials: T
authors.lastname: Shafique
authors.forename: F J
authors.initials: FJ
authors.lastname: Schoen
authors.forename: W
authors.initials: W
authors.lastname: Grossman
authors.forename: R M
authors.initials: RM
authors.lastname: Weintraub
mesh_terms: D000818:Animals; D003097:Collateral Circulation; D003250:Constriction; D003326:Coronary Circulation; D004730:Endothelium, Vascular / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D006439:Hemodynamics; D008297:Male; D008833:Microcirculation; D008854:Microscopy, Electron; D010477:Perfusion; D013552:Swine