title: [Effects of atropine on acetylcholine levels in the rat iris-ciliary body].
abstract: In an attempt to estimate the in vivo acetylcholine level in rat iris-ciliary body, animals were sacrificed by microwave irradiation which rapidly inactivates metabolic enzymes. Acetylcholine levels in the iris-ciliary body were determined by high-performance liquid chromatography with electrochemical detection. The acetylcholine level in rat iris-ciliary body per gram tissue protein was 58.8 +/- 5.0 nmol/g (mean +/- SEM, n = 9). A single instillation of 5 microliters of an 0.05% atropine to one eye of a rat decreased acetylcholine level in the iris-ciliary body by 17% half an hour after administration (p less than 0.02).
authors.affiliation: Hiromatsu Eye Clinic, Ichihara, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hiromatsu
authors.forename: M
authors.initials: M
authors.lastname: Araie
authors.forename: K
authors.initials: K
authors.lastname: Fujimori
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000008:administration & dosage / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D002924:Ciliary Body / Q000187:drug effects* / Q000378:metabolism; D007498:Iris / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001285:Atropine; D000109:Acetylcholine