[The effect of monoclonal and polyclonal antibodies to peroxidase on combined peroxidase oxidation of 4-iodophenol with luminol and 4-aminoantipyrine].
The kinetics of peroxidase-dependent cooxidation for two substrate pairs [p-iodophenol + 4-aminoantipyrine (AAP) and p-iodophenol + luminol was studied both in the absence and presence of polyclonal antibodies (polyAB), three types of peroxidase-specific monoclonal antibodies (monoAB) and their double or triple mixtures in a wide range of H2O2 concentrations (0.01-10.0 mM). MonoAB 2C, 3E and 9D at concentrations of 0.05-500 nM inhibited the cooxidation of p-iodophenol + AAP at H2O2 concentration above 1.0 mM but activated the cooxidation of p-iodophenol + luminol. The double and triple mixtures of monoAB activated the cooxidation of p-iodophenol + AAP at the same H2O2 concentrations without any effect on the p-iodophenol + luminol cooxidation. PolyAB activated the cooxidation of p-iodophenol + AAP more effectively and only slightly activated (or inhibited) that of p-iodophenol + luminol. PolyAB diminished the values of rate constants for the interaction of the peroxidase active intermediates, E1 and E2, with p-iodophenol, AAP or luminol. Possible modes of monoAB and polyAB effects on the two substrate pair cooxidation are discussed.
Litvinchuk
A V
AV
Metelitsa
D I
DI
Savenkova
M I
MI
Cherednikova
T V
TV
Kim
B B
BB
Pisarev
V V
VV
D000675:Ampyrone / Q000737:chemistry*; D000911:Antibodies, Monoclonal; D007462:Iodobenzenes / Q000737:chemistry*; D007700:Kinetics; D008165:Luminol / Q000737:chemistry*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007462:Iodobenzenes; D000675:Ampyrone; C050181:4-iodophenol; D008165:Luminol; D010544:Peroxidases
Vliianie monoklonal'nykh i poliklonal'nykh antitel k peroksidaze na sopriazhennoe pereoksidaznoe okislenie 4-iodfenola s liuminolom i 4-aminoantipirinom.