The dynamics of beta-endorphin release by the hypothalamic nuclei and pituitary gland in sheep under physiological and stress condition.
The secretion of beta-endorphin (beta-End) by the infundibular nuclei--median eminence (IN-ME) and paraventricular nuclei (PVN) of the hypothalamus and the anterior lobe (AL) of the pituitary gland was determined in 18 anestrous ewes by RIA assay of this opioid in perfusates collected from these formations. Perfusion with Ringer-Locke solution was carried out on the animals under resting and stress-full conditions, using a pushpull cannula method. Electrical footshocks were used as stress stimuli. Two series of perfusion experiments were performed. In the first, the perfusions were carried out over two hours and perfusates were collected before and during stress for 1 h. In the second series of experiments the dynamics of the release of the opioid by the IN-ME and the pituitary gland were followed by collecting five or six perfusates in 20 min fractions before and during stress, respectively. The first series of experiments showed that the concentrations of beta-End in perfusates from the pituitary gland were much higher than those from the hypothalamic nuclei and that the concentrations of this opioid during stressing rose significantly only in perfusates from the IN-ME nuclei. In the second series of experiments the release of beta-End from the IN-ME and pituitary gland was altered by stress. The shift was very characteristic in that the concentrations of the opioid during stimulation initially significantly rose and then gradually declined. The concentration of the opioid in the peripheral blood also rose at the beginning of stimulation and declined as stimulation continued.(ABSTRACT TRUNCATED AT 250 WORDS)
Domański
E
E
Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jabłonna.
Ostrowska
A
A
Rutkowski
J
J
Luboińska
U
U
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism* / Q000502:physiology; D004558:Electric Stimulation; D005260:Female; D008473:Median Eminence / Q000378:metabolism* / Q000502:physiology; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism* / Q000502:physiology; D010477:Perfusion; D010902:Pituitary Gland / Q000378:metabolism* / Q000502:physiology; D011863:Radioimmunoassay; D012146:Rest / Q000502:physiology; D012756:Sheep; D013312:Stress, Physiological; D013997:Time Factors; D001615:beta-Endorphin / Q000097:blood*
D001615:beta-Endorphin