[The cytochemical demonstration of glycoproteins and glycolipids in Sarcocystis muris sarcocysts].
An electron cytochemical study of glycoproteins and glycolipids was made for the mature sarcocysts of Sarcocystis muris. Glycoprotein structures as branched fibrilles were seen on the surface of the sarcocyst wall. The fibrillar and granular glycoprotein structures were found in the ground substance of sarcocysts near the cyst wall and in the septae. In the plasmalemma of two types of cyst stages (merozoites and intermediate cells), glycoprotein fibrillar structures were revealed connecting these two cell types with each other. The third type cyst stages, i.e. the metrocytes, are situated separately without any fibrillar connections between them and other cyst stages being observed. This question is discussed in terms of the problem of cytodifferentiation. The fibrillar and granular glycoprotein material is scattered over the cytoplasm of the cyst stages, being especially concentrated in micronemes, rhoptries and around amylopectin granules. The control ultrathin sections were treated with saliva or pronase for the aims of protein identification in the material under study. In addition to glycoprotein, some glycolipids material was detected in the sarcocysts in the form of drops surrounded with thin glycoproteinaceous layers. Glycolipids were found in the ground substance of sarcocysts near the cyst stages and in the parasite cell cytoplasm around the micronemes and rhoptries. The data obtained are discussed in connection with the functional role glycoproteins and glycolipids play in S. muris.
Radchenko
A I
AI
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D006017:Glycolipids / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism* / Q000648:ultrastructure; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009132:Muscles / Q000469:parasitology*; D012522:Sarcocystis / Q000378:metabolism* / Q000648:ultrastructure; D012523:Sarcocystosis / Q000469:parasitology
D006017:Glycolipids; D006023:Glycoproteins
Tsitokhimicheskoe vyiavlenie glikoproteinov i glikolipidov v sarkotsistakh Sarcocystis muris.