Microscopic evaluation of the dansyl chloride test.
The classical dansyl chloride test relies on visual assessment of the extinction of fluorescence in time. We introduce a microscopic and morphometric rating of this test. We show that soaps may remove the fluorescing dye from corneocytes. Therefore caution should be taken in interpreting fluorescence extinction only as an estimate of the stratum corneum renewal.
Piérard
G E
GE
Department of Dermatopathology, University of Liège, Belgium.
D003358:Cosmetics / Q000494:pharmacology*; D003619:Dansyl Compounds / Q000493:pharmacokinetics; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D009824:Ointments; D010328:Patch Tests; D012867:Skin / Q000166:cytology* / Q000187:drug effects*; D012915:Soaps / Q000494:pharmacology*; D013997:Time Factors
D003358:Cosmetics; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D009824:Ointments; D012915:Soaps; C029038:dansyl chloride