Influence of attractants and repellents on methyl group turnover on methyl-accepting chemotaxis proteins of Bacillus subtilis and role of CheW.
The ability of attractants and repellents to affect the turnover of methyl groups on the methyl-accepting chemotaxis proteins (MCPs) was examined for Bacillus subtilis. Attractants were found to cause an increase in the turnover of methyl groups esterified to the MCPs, while repellents caused a decrease. These reactions do not require CheW. However, a cheW null mutant exhibits enhanced turnover in unstimulated cells. Assuming that the turnover of methyl groups on the MCPs reflects a change in the activity of CheA, these results suggest that the activation of CheA via chemoeffector binding at the receptor does not require CheW.
Hanlon
D W
DW
Department of Biochemistry, College of Medicine, University of Illinois, Urbana 61801.
Carpenter
P B
PB
Ordal
G W
GW
D001412:Bacillus subtilis / Q000187:drug effects / Q000502:physiology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000378:metabolism*; D002087:Butyrates / Q000494:pharmacology; D002630:Chemotactic Factors / Q000502:physiology*; D002633:Chemotaxis; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D014316:Tritium
D001426:Bacterial Proteins; D002087:Butyrates; D002630:Chemotactic Factors; D014316:Tritium; C051236:CheW protein, Bacteria; D008715:Methionine