Regulation of the natural killer cell response to interferon-alpha by biogenic amines.
Monocytes, recovered from human peripheral blood by counter-current centrifugal elutriation (CCE), suppressed baseline natural killer (NK) cell cytotoxicity (NKCC) and rendered NK cells resistant to activation of cytotoxicity by human recombinant interferon-alpha (IFN-alpha) by a cell contact-dependent mechanism. Monocyte-induced suppression of resting and IFN-activated NK cells was abrogated by the biogenic amines histamine [via H2-type receptors (H2R)] and serotonin [via 5-HT1A-type receptors (5-HT1AR)]. Our data are suggestive of a monocyte/NK cell interaction that is subject to regulation by biogenic amines.
Hellstrand
K
K
Department of Clinical Virology, University of Göteborg, Sweden.
Kylefjord
H
H
Asea
A
A
Hermodsson
S
S
D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000502:physiology; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004357:Drug Synergism; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects*; D009000:Monocytes / Q000502:physiology*; D010641:Phenotype; D011994:Recombinant Proteins; D012701:Serotonin / Q000494:pharmacology*; D013601:T-Lymphocytes; D014407:Tumor Cells, Cultured
D007370:Interferon Type I; D011994:Recombinant Proteins; D012701:Serotonin; D006632:Histamine