Apolipoprotein A-1 interacts with the N-terminal fusogenic domains of SIV (simian immunodeficiency virus) GP32 and HIV (human immunodeficiency virus) GP41: implications in viral entry.
Previous studies showed that apoA1, the major protein component of HDL (High Density Lipoprotein), inhibited HIV infectivity and virus-induced syncytia formation. The mechanism of inhibition is unknown. We bring here evidence that the amphipathic helices of apoA1 interact with the N-terminal peptides of SIV gp32 and HIV gp41. These peptides have been shown to be associated with the initial steps of the fusion between the host cell and the virus. Binding of apoA1 to these peptides prevents the insertion of the fusogenic domains into the cell membrane and inhibits the fusion and the entry of the virus into the host cell.
Martin
I
I
Laboratoire de Chimie-Physique des Macromolécules aux Interfaces, Université Libre de Bruxelles, Belgium.
Dubois
M C
MC
Saermark
T
T
Ruysschaert
J M
JM
D000595:Amino Acid Sequence; D016632:Apolipoprotein A-I / Q000378:metabolism*; D015686:Gene Products, env / Q000378:metabolism*; D006678:HIV / Q000502:physiology*; D015700:HIV Envelope Protein gp41 / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008081:Liposomes; D008561:Membrane Fusion; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism; D010743:Phospholipids; D011485:Protein Binding; D015682:Retroviridae Proteins, Oncogenic / Q000378:metabolism*; D015302:Simian Immunodeficiency Virus / Q000502:physiology*; D014760:Viral Fusion Proteins
D016632:Apolipoprotein A-I; D015686:Gene Products, env; D015700:HIV Envelope Protein gp41; D008081:Liposomes; D010446:Peptide Fragments; D010743:Phospholipids; D015682:Retroviridae Proteins, Oncogenic; D014760:Viral Fusion Proteins; C059580:transmembrane protein, Simian immunodeficiency virus