Morphological differences in crystals of multiple forms of yeast transketolase.
Monocrystals of three individual multiple forms of yeast transketolase (A, B and C) differing in their thermostability have been obtained. Ammonium sulfate was used as a precipitating agent. Crystals of the mentioned forms were found to possess different morphology and stability during storage. Single crystals growing from the enzyme form C within 4-7 days were subsequently destroyed. Simultaneously, in the preparation, microcrystals started to grow in a great number. They were found to correspond morphologically to crystals obtained from transketolase A. A possibility of interconversions of the enzyme forms in sequence C----A----B is discussed.
Kuimov
A N
AN
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
Konareva
N V
NV
Kochetov
G A
GA
D002482:Cellulose / Q000031:analogs & derivatives; D002845:Chromatography; D003460:Crystallization; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014174:Transketolase / Q000737:chemistry* / Q000378:metabolism; D014961:X-Ray Diffraction
D002482:Cellulose; C008881:phosphocellulose; D014174:Transketolase