Functional expression of a plant plasma membrane transporter in Xenopus oocytes.
A full-length cDNA clone for the H+/hexose co-transporter (STP1) from Arabidopsis thaliana has been transcribed in vitro and the mRNA injected into Xenopus oocytes. Under optimized conditions, oocytes injected with the STP1 mRNA accumulated 3-O-[methyl-14C]glucose at rates of more than a 1000-fold greater than water-injected control oocytes. A hexose-elicited depolarization of the oocyte membrane potential was demonstrated, and uptake was shown to be stimulated by low external pH, confirming the activity of a H+/hexose co-transport system. This is the first example of the functional expression of a plant membrane transporter in oocytes.
Boorer
K J
KJ
Department of Biochemistry and Physiology, AFRC-IACR, Harpenden, Herts., UK.
Forde
B G
BG
Leigh
R A
RA
Miller
A J
AJ
D019325:3-O-Methylglucose; D000818:Animals; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008757:Methylglucosides / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009865:Oocytes / Q000378:metabolism*; D010944:Plants / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection; D014982:Xenopus laevis
D008757:Methylglucosides; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D019325:3-O-Methylglucose