title: Expression of a heterodimeric Fab antibody protein in one cloning step.
abstract: A new method is presented for creating antibody expression libraries in Escherichia coli. Rather than perform two cloning steps to express the heavy and light chains of the antigen binding domain, we have used a fusion-PCR method to link the coding regions for heavy and light chain sequences in a single DNA molecule prior to vector ligation. This greatly simplifies the construction of antibody expression clones.
authors.affiliation: Stratagene, La Jolla, CA 92037.
authors.forename: R L
authors.initials: RL
authors.lastname: Mullinax
authors.forename: E A
authors.initials: EA
authors.lastname: Gross
authors.forename: B N
authors.initials: BN
authors.lastname: Hay
authors.forename: J R
authors.initials: JR
authors.lastname: Amberg
authors.forename: M M
authors.initials: MM
authors.lastname: Kubitz
authors.forename: J A
authors.initials: JA
authors.lastname: Sorge
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002675:Child, Preschool; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA; D004926:Escherichia coli; D015698:Genomic Library; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D007140:Immunoglobulin Fab Fragments; D004247:DNA