A rapid procedure for production of human basic fibroblast growth factor in Escherichia coli cells.
Human basic fibroblast growth factor has been expressed in Escherichia coli cells at a level of 2-3 mg/l culture, using a rapid procedure which requires only simple DNA manipulative work. The recombinant material has the same potency as natural basic fibroblast growth factor from bovine pituitary glands.
Ke
Y
Y
Department of Biochemistry, University of Liverpool, UK.
Wilkinson
M C
MC
Fernig
D G
DG
Smith
J A
JA
Rudland
P S
PS
Barraclough
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000235:genetics*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D014169:Transformation, Bacterial / Q000235:genetics
D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2