A competitive ELISA using anti-N monoclonal antibodies for specific detection of rinderpest antibodies in cattle and small ruminants.
A competitive ELISA (C-ELISA) using monoclonal antibodies (mAbs) which bind to the nucleo-protein (NP) of rinderpest virus (RPV) for detection of RPV antibodies in cattle and small ruminant sera is described. Unlike virus neutralisation test (VNT), this test using mAb IVB2-4, can detect specific RPV antibodies without showing a cross-reaction with antibodies to peste-des-petits ruminants-virus (PPRV); by contrast, when mAb VE4-1 is used the test detects both RPV and PPRV antibodies, including low levels of antibodies that can be found in sera containing maternal antibodies. Although antibodies to the PPRV 75-1 strain are also detected with mAb 51-5-6, the test is suitable for assessing the immune status of cattle against the Rinderpest Old Kabete (RBOK) strain. The results from a panel of sera with a known status of vaccination provide evidence for a highly significant correlation between C-ELISA and VNT. This test may be a useful tool for a standardized and accurate determination of the immunity status of both cattle and small ruminants.
Libeau
G
G
Institut d'Elevage et de Médecine Vétérinaire des Pays Tropicaux/CIRAD; Service de Pathologie infectieuse, Maisons-Alfort, France.
Diallo
A
A
Calvez
D
D
Lefèvre
P C
PC
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D001667:Binding, Competitive; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D009500:Neutralization Tests; D012302:Rinderpest virus / Q000276:immunology*; D012680:Sensitivity and Specificity; D012756:Sheep; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014765:Viral Vaccines