Novel solid-state fluorodensitometric method for the determination of haptens in protein-hapten conjugates. Demonstration with a toxic glycoside of Cleistanthus collinus.
A method is reported for the solid-state fluorodensitometric determination of haptens in hapten-protein conjugates. The applicability of the method is shown using the bovine serum albumin conjugate of cleistanthin B, a toxic glycoside of the plant Cleistanthus collinus. This non-destructive technique, which is relatively simple, sensitive, rapid and versatile, can be used for the determination of protein conjugates of fluorogenic haptens.
Ragupathi
G
G
R&D Division, Forensic Sciences Department, Madras, India.
Prabhasankar
P
P
Sekharan
P C
PC
Annapoorani
K S
KS
Damodaran
C
C
D002855:Chromatography, Thin Layer; D006027:Glycosides / Q000378:metabolism*; D006241:Haptens / Q000378:metabolism*; D017705:Lignans; D010947:Plants, Toxic / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D006027:Glycosides; D006241:Haptens; D017705:Lignans; C010088:cleistanthin; D012710:Serum Albumin, Bovine