Monoclonal antibodies to the nonpeptide angiotensin II receptor antagonist, losartan.
Two murine monoclonal antibodies were produced to losartan (DuP 753), a nonpeptide angiotensin II receptor antagonist. Using a solid phase competitive enzyme-linked immunosorbent assay (ELISA), each antibody was examined for its ability to bind to a set of losartan analogs that differ structurally in varying degrees. Both antibodies distinguished fine structural changes in the analogs, particularly at the R5 position of the imidazole ring. No cross-reactivity towards either antibody was observed with the natural ligand angiotensin II, the peptide antagonist saralasin, or the AT2 selective nonpeptide antagonist PD123177.
Reilly
T M
TM
Du Pont Merck Pharmaceutical Company, Wilmington, DE 19880-0400.
Christ
D D
DD
Duncia
J V
JV
Pierce
S K
SK
Timmermans
P B
PB
D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D057911:Angiotensin Receptor Antagonists; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001713:Biphenyl Compounds / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007093:Imidazoles / Q000276:immunology*; D019808:Losartan; D051379:Mice; D008807:Mice, Inbred BALB C; D011725:Pyridines / Q000276:immunology; D012504:Saralasin / Q000276:immunology / Q000378:metabolism; D013329:Structure-Activity Relationship; D013777:Tetrazoles / Q000276:immunology*
D057911:Angiotensin Receptor Antagonists; D000911:Antibodies, Monoclonal; D001713:Biphenyl Compounds; D007093:Imidazoles; D011725:Pyridines; D013777:Tetrazoles; D000804:Angiotensin II; C066253:PD 123177; D012504:Saralasin; D019808:Losartan