title: New mRNA probes for hippocampal responses to entorhinal cortex lesions in the adult male rat: a preliminary report.
abstract: Three new mRNA responses were found in the hippocampus of the adult male rat after entorhinal cortex lesions (ECL) that induce synaptic reorganization. Hippocampus cDNA libraries were screened by a subtractive hybridization strategy designed to detect ECL-induced mRNAs. Partial sequencing showed clones with similarities to mouse vimentin, ferritin, and polypeptide 7B-2. A sequence similar to mouse SNAP-25 sequence was also detected. Using a mouse SNAP-25 probe, rat SNAP-25 mRNA increased in the hippocampus after ECL.
authors.affiliation: Neurogerontology Division, Andrus Gerontology Center, Los Angeles, California.
authors.forename: J R
authors.initials: JR
authors.lastname: Day
authors.forename: B H
authors.initials: BH
authors.lastname: Min
authors.forename: N J
authors.initials: NJ
authors.lastname: Laping
authors.forename: G
authors.initials: G
authors.lastname: Martin
authors.forename: H H
authors.initials: HH
authors.lastname: Osterburg
authors.forename: C E
authors.initials: CE
authors.lastname: Finch
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005293:Ferritins / Q000235:genetics; D007839:Functional Laterality; D015723:Gene Library; D006624:Hippocampus / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010957:Plasmids; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012689:Sequence Homology, Nucleic Acid; D050825:Synaptosomal-Associated Protein 25; D014746:Vimentin / Q000235:genetics
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D015347:RNA Probes; D012333:RNA, Messenger; C494209:Snap25 protein, mouse; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25; C075455:TB-2 polypeptide; D014746:Vimentin; D005293:Ferritins