Plasmids encoding for erythromycin ribosomal methylase of Staphylococcus epidermidis and Staphylococcus simulans.
Two 1.7 Md plasmids of Staphylococcus epidermidis and three ones of Staphylococcus simulans determining inducible macrolide-lincosamide resistance are identical as judged by restriction endonuclease fingerprinting. These plasmids designated pEI2101, pEI9105, pE1107, pEI1108 and pEI6104, respectively, belong to the incompatibility group 12. Dot-blot hybridization by photobiotin-labelled gene probe developed from S. aureus erythromycin ribosomal methylase gene showed cross hybridization between methylase-coding reference plasmids and the tested ones. The examined plasmids proved to be no transmissible in mating experiments into S. aureus recipients.
Barcs
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B. Johan National Institute of Hygiene, Budapest, Hungary.
Jánosi
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D004352:Drug Resistance, Microbial / Q000235:genetics; D004917:Erythromycin; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D012270:Ribosomes / Q000201:enzymology*; D013210:Staphylococcus / Q000201:enzymology / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000201:enzymology / Q000235:genetics*
D004917:Erythromycin; D008780:Methyltransferases