title: A rapid-kinetic study of the class C beta-lactamase of Enterobacter cloacae 908R.
abstract: The individual rate constants for acylation and deacylation (k2 and k3, respectively) of the class C beta-lactamase of Enterobacter cloacae 908R by ampicillin and carbenicillin have been determined. For several other beta-lactams, the value of k2 was too high to be determined and the k2/k3 ratio could be larger than 10,000. Branched pathways were also shown to occur with several penicillins and cephalosporins.
authors.affiliation: Laboratoire d'enzymologie, Université de Liège, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Monnaie
authors.forename: R
authors.initials: R
authors.lastname: Virden
authors.forename: J M
authors.initials: JM
authors.lastname: Frère
mesh_terms: D016972:Enterobacter cloacae / Q000201:enzymology*; D007700:Kinetics; D013379:Substrate Specificity; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases