title: Nucleotide sequence and coding strategy of the Uukuniemi virus L RNA segment.
abstract: The complete nucleotide sequence of the L RNA segment of Uukuniemi virus has been determined from cloned cDNA. The L RNA is 6423 nucleotides in length, and is of negative polarity. The viral-complementary RNA contains a single large open reading frame of 2104 codons which corresponds to the L protein (M(r) 241039). Comparison with the L protein sequences of other members of the Bunyaviridae showed homology with the Rift Valley fever phlebovirus L protein (38% amino acid identity), but no detectable similarity with bunyavirus, hantavirus or tospovirus L proteins. These data lend further support for the recent reclassification of uukuviruses and phleboviruses into the same genus, Phlebovirus, in the family Bunyaviridae. The L RNA sequence completes the determination of the Uukuniemi virus genome: since the M RNA segment is 3229 and the S RNA segment 1720 nucleotides, the whole genome comprises 11372 nucleotides.
authors.affiliation: Institute of Virology, University of Glasgow, U.K.
authors.forename: R M
authors.initials: RM
authors.lastname: Elliott
authors.forename: E
authors.initials: E
authors.lastname: Dunn
authors.forename: J F
authors.initials: JF
authors.lastname: Simons
authors.forename: R F
authors.initials: RF
authors.lastname: Pettersson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002043:Bunyaviridae / Q000235:genetics*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry*
chemical_list: D012367:RNA, Viral