Bioassay of bovine interleukin-1-like activity.
A bioassay for bovine interleukin-1 (IL1) activity is described. The assay is based on the IL1-stimulated proliferation of a mouse T-lymphocyte cell line, D10(N4)M. Bovine mononuclear cells stimulated with lipopolysaccharide produce an interleukin-1-like activity which stimulated the growth of the D10(N4)M cell line in a dose-dependent manner. The stimulatory activity was neutralised by a combination of both anti-human IL1 alpha and anti-human IL1 beta sera. The quantity of IL1-like activity released from the mononuclear cells increased asymptotically with increasing lipopolysaccharide dose.
Winstanley
F P
FP
Institute of Biochemistry, Glasgow Royal Infirmary.
Eckersall
P D
PD
D000818:Animals; D001681:Biological Assay; D002417:Cattle; D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007375:Interleukin-1 / Q000032:analysis* / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D007375:Interleukin-1