Amino alcohol modulation of hippocampal acetylcholine release.
The synthesis and release of 3H-acetylcholine was measured in hippocampal slices of adult rat brain following acute in vitro exposure to ethanolamine. Evoked release of 3H-acetylcholine was elevated by 60-70% but 3H-acetylcholine synthesis was unaffected. Other amino alcohols were also found to significantly increase evoked 3H-acetylcholine release. The effect may be stereochemically mediated since only one of four possible propanolamine configurations, R-alaninol, was active. The most potent compound tested was R-prolinol which showed an EC50 nearly 10-fold lower than that of either R-alaninol or ethanolamine; S-prolinol was inactive. Slices taken from adult rats which had been fed active compounds for two weeks also exhibited enhancements in evoked 3H-acetylcholine release. These results indicate that amino alcohols modulate acetylcholine release in the rat hippocampus.
Bostwick
J R
JR
Department of Neurology, Baylor College of Medicine, Houston, Texas 77030.
Abbe
R
R
Sun
J
J
Appel
S H
SH
D000109:Acetylcholine / Q000378:metabolism*; D000605:Amino Alcohols / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D019856:Ethanolamine; D004983:Ethanolamines / Q000032:analysis / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
D000605:Amino Alcohols; D004983:Ethanolamines; D019856:Ethanolamine; D000109:Acetylcholine