Fluorescence body of bovine spermatozoa represents Y-chromosome.
The fluorescence body test would be an inexpensive method of quantifying the Y-chromosomes of mammalian spermatozoa, and could be used to monitor the enrichment of X- and Y-spermatozoa. However, a definitive proof that the fluorescence bodies are markers of the Y-chromosome, is available only for humans and gorillas. In the study described here it is demonstrated for the first time in cattle that the fluorescence bodies of spermatozoa represent the Y-chromosomes. The evidence is furnished by a combination of the fluorescence staining (quinacrine mustard) with the in-situ-hybridization using bovine male-specific DNA fragments. The incidence of f-bodies corresponded with autoradiographic grains caused by the tritiated Y-specific DNA in the examined 144 single cells (75, 5, and 88% agreement).
Blottner
S
S
Center of Vertebrate Research, Berlin, Germany.
Schwerin
M
M
Pitra
C
C
Reinsch
A
A
Jewgenow
K
K
D000818:Animals; D002417:Cattle; D015342:DNA Probes; D005456:Fluorescent Dyes; D008297:Male; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D013094:Spermatozoa / Q000648:ultrastructure*; D014998:Y Chromosome / Q000648:ultrastructure*
D015342:DNA Probes; D005456:Fluorescent Dyes