title: The broad diversity of neutral and sialylated oligosaccharides derived from human salivary mucins.
abstract: Mucin glycopeptides were prepared from the salivary mucins of 20 healthy donors with blood group O. The carbohydrate chains of the high-molecular-weight mucins were released by alkaline borohydride treatment. Neutral and monosialylated oligosaccharide-alditols were purified by ion-exchange chromatography, gel filtration, and HPLC. The structures of the oligosaccharide-alditols were determined by high-resolution 1H-NMR spectroscopy in combination with fast atom bombardment mass spectrometry and methylation analysis. Thirty-seven oligosaccharide-alditols were characterized and illustrate the extreme diversity of the salivary mucins carbohydrate chains. This diversity might represent a mosaic of bacterial adhesion sites and be involved in the early events of the nonimmune defense of the oral cavity. Among these 37 oligosaccharide-alditols, 31 have not been previously described in human saliva and five of these are novel structures: [formula: see text]
authors.affiliation: Unité des Protéines, Institut National de la Santé et de la Recherche Médicale No. 16, Lille, France.
authors.forename: A
authors.initials: A
authors.lastname: Klein
authors.forename: C
authors.initials: C
authors.lastname: Carnoy
authors.forename: J M
authors.initials: JM
authors.lastname: Wieruszeski
authors.forename: G
authors.initials: G
authors.lastname: Strecker
authors.forename: A M
authors.initials: AM
authors.lastname: Strang
authors.forename: H
authors.initials: H
authors.lastname: van Halbeek
authors.forename: P
authors.initials: P
authors.lastname: Roussel
authors.forename: G
authors.initials: G
authors.lastname: Lamblin
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009077:Mucins / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011402:Pronase / Q000378:metabolism; D012463:Saliva / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013402:Sugar Alcohols / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols; C016735:sialooligosaccharides; D011402:Pronase