Serological aspects of coeliac disease.
We have evaluated the sensitivity and specificity of serum anti-gliadin, anti-reticulin and anti-endomysium antibody determinations for the diagnosis of coeliac disease. Serum samples from 54 children and 9 adults with untreated coeliac disease were analysed for anti-gliadin antibodies and compared to the results obtained for 111 non coeliac controls (72 children, 39 adults) and 221 healthy blood donors. This yields sensitivities of 87% and 86% for IgA and IgG antibodies. Specificities were respectively of 95.5% and 91%. Anti-reticulin and anti-endomysium IgA were analysed in a more limited number of sera. Specificity of both tests was of 100% for the IgA class of antibody. However sensitivity was only of 64% for anti-reticulin IgA but was of 100% for anti-endomysium IgA. These results indicate that combination of anti-gliadin antibodies (IgA and IgG), anti-reticulin and anti-endomysium IgA may be recommended as screening tests to better select patients suspected of coeliac disease for jejunal biopsy.
Mascart-Lemone
F
F
Dept. of Immunology, Brugmann Hospital, Brussels, Belgium.
Van den Broeck
J
J
Cadranel
S
S
Colombel
J F
JF
D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000302:isolation & purification*; D002446:Celiac Disease / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003238:Connective Tissue / Q000276:immunology; D005903:Gliadin / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000302:isolation & purification; D007223:Infant; D008875:Middle Aged; D012155:Reticulin / Q000276:immunology; D012680:Sensitivity and Specificity
D001323:Autoantibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D012155:Reticulin; D005903:Gliadin