Analysis of tubulin isoforms by two-dimensional gel electrophoresis using SDS-polyacrylamide gel electrophoresis in the first dimension.
A two-dimensional electrophoretic system using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) in the first dimension and isoelectric focusing (IEF) in the second dimension was devised. In spite of its simplicity, this method could show a markedly high resolution for tubulin isoforms and moreover could classify them into alpha- or beta-tubulin as a two-dimensional profile. With this method, seven alpha- and four beta-tubulin isoforms could be detected within axoneme from Tetrahymena cilia. Moreover this method could also resolve tubulin isoforms from the rabbit brain. These results indicate that the present two-dimensional gel electrophoresis is a useful tool for the electrophoretic analysis of tubulin isoforms from various sources.
Nakamura
K
K
Department of Living Sciences, Hiroshima Women's University, Japan.
Okuya
Y
Y
Katahira
M
M
Yoshida
S
S
Wada
S
S
Okuno
M
M
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology; D002923:Cilia / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D007525:Isoelectric Focusing; D011817:Rabbits; D013768:Tetrahymena / Q000737:chemistry / Q000648:ultrastructure; D014404:Tubulin / Q000032:analysis* / Q000737:chemistry
D014404:Tubulin