title: Competitive exclusion of diarrheagenic Escherichia coli (ETEC) from human enterocyte-like Caco-2 cells by heat-killed Lactobacillus.
abstract: Diarrheagenic Escherichia coli (ETEC) bearing CFA/I or CFA/II adhesive factors specifically adhere onto the brush border of the polarized epithelial human intestinal Caco-2 cells in culture. Heat-killed Lactobacillus acidophilus strain LB, that adheres onto Caco-2 cells, inhibits diarrheagenic Escherichia coli adhesion in a concentration-dependent manner. Since the L. acidophilus does not express ETEC-CFA adhesive factors, it can be postulated that the heat-killed L. acidophilus LB cells inhibit diarrheagenic E. coli attachment by steric hindrance of the human enterocytic ETEC receptors.
authors.affiliation: Département de Microbiologie et Immunologie, UFR Sciences Pharmaceutiques Paris XI, Châtenay-Malabry, France.
authors.forename: G
authors.initials: G
authors.lastname: Chauvière
authors.forename: M H
authors.initials: MH
authors.lastname: Coconnier
authors.forename: S
authors.initials: S
authors.lastname: Kerneis
authors.forename: A
authors.initials: A
authors.lastname: Darfeuille-Michaud
authors.forename: B
authors.initials: B
authors.lastname: Joly
authors.forename: A L
authors.initials: AL
authors.lastname: Servin
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001667:Binding, Competitive; D002460:Cell Line; D016764:Cell Polarity; D003967:Diarrhea / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D004926:Escherichia coli / Q000472:pathogenicity*; D005069:Evaluation Studies as Topic; D006801:Humans; D007422:Intestines / Q000473:pathology; D007778:Lactobacillus / Q000378:metabolism; D008871:Microvilli / Q000502:physiology