Ligation-free gene synthesis by PCR: synthesis and mutagenesis at multiple loci of a chimeric gene encoding ompA signal peptide and hirudin.
Majumder
K
K
International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D005813:Genes, Synthetic; D006629:Hirudins / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D006629:Hirudins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins