Production of v-Myb and c-Myb in insect cells infected with recombinant baculoviruses.
Recombinant baculoviruses expressing the v-myb and c-myb genes in infected insect cells were constructed. The electrophoretic mobilities of their immunoreactive products were the same as those of the authentic Myb proteins from chicken cells. The system provides a convenient source of relatively large amounts of v-Myb or c-Myb for in vitro binding studies.
Vranovský
K
K
Czechoslovak Academy of Sciences, Institute of Molecular Genetics, Prague.
Bartůnĕk
P
P
Cermáková
V
V
Forstová
J
J
Dvorák
M
M
Doskocil
J
J
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000235:genetics*; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D015151:Immunoblotting; D009036:Moths / Q000382:microbiology; D020626:Oncogene Proteins v-myb; D010957:Plasmids / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D020598:Proto-Oncogene Proteins c-myb; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis / Q000235:genetics*
D020626:Oncogene Proteins v-myb; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D011994:Recombinant Proteins; D015682:Retroviridae Proteins, Oncogenic