Muramyl dipeptide-induced changes in murine splenocyte responses to concanavalin A.
The effect of muramyl dipeptide (MDP) on Con A-stimulated activation of murine spleen cells was studied. MDP was found to enhance or suppress the proliferative response of splenocytes when different concentrations of Con A were used. MDP was shown to change the IL-2 content in culture supernatants of stimulated cells and to influence IL-2-dependent proliferation of Con A-blasts. A high degree of correlation was found between the proliferation of Con A-blasts and the expression of IL-2 receptors on Con A-blasts. This correlation, however, disappeared in the presence of MDP. The effects of MDP were shown to depend on the level of initial cell activity or rather on conditions leading to a given initial activity of cells.
Dozmorov
I M
IM
Department of Immunology, Shemyakin Institute of Bioorganic Chemistry, Moscow, USSR Academy of Sciences.
Bagaeva
L V
LV
Kuzin
I I
II
Sapozhnikov
A M
AM
Svirshchevskaya
E V
EV
Andronova
T M
TM
Petrov
R V
RV
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology*; D000818:Animals; D003208:Concanavalin A; D005260:Female; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013154:Spleen / Q000187:drug effects* / Q000276:immunology
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D003208:Concanavalin A; D000119:Acetylmuramyl-Alanyl-Isoglutamine