L-696,474, a novel cytochalasin as an inhibitor of HIV-1 protease. II. Isolation and structure.
A novel HIV-1 protease inhibitor, L-696,474 (C30H39NO4, 477), was isolated from the fermentations of the fungus Hypoxylon fragiforme (ATCC 20995, MF5511) and purified by silica gel chromatography followed by crystallization. Spectroscopic studies have shown the competitive inhibitor L-696,474 to be a novel cytochalasin. Two related novel cytochalasins were also isolated and had no effect on the enzyme.
Ondeyka
J
J
Department of Natural Products Chemistry, Merck Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065-0900.
Hensens
O D
OD
Zink
D
D
Ball
R
R
Lingham
R B
RB
Bills
G
G
Dombrowski
A
A
Goetz
M
M
D001203:Ascomycota / Q000737:chemistry*; D003572:Cytochalasins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017320:HIV Protease Inhibitors; D054833:Isoindoles
D003572:Cytochalasins; D017320:HIV Protease Inhibitors; D054833:Isoindoles; C075543:18-dehydroxycytochalasin H; C034891:cytochalasin H