Preparative capillary electrophoresis based on adsorption of the solutes (proteins) onto a moving blotting membrane as they migrate out of the capillary.
A micropreparative capillary electrophoresis apparatus equipped with a new type of fraction collection device is described: solutes, such as proteins, are adsorbed onto a moving blotting membrane (for instance a polyvinylidene difluoride membrane) as they migrate electrophoretically out of the capillary. The adsorbed proteins are visualized by conventional protein staining methods or by fluorescent labeling. Specific identification of separated components by an immunological technique is demonstrated. The method also offers the potential to analyze proteins and peptides collected on the membrane by gas phase sequencing and mass spectrometry.
Eriksson
K O
KO
Department of Biochemistry, University of Uppsala, Biomedical Center, Sweden.
Palm
A
A
Hjertén
S
S
D000327:Adsorption; D000818:Animals; D003101:Collodion; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008567:Membranes, Artificial; D011506:Proteins / Q000032:analysis*
D008567:Membranes, Artificial; D011506:Proteins; D003101:Collodion