Dietary vitamin E and the attenuation of early lesion development in modified Watanabe rabbits.
Modified Watanabe heritable hyperlipidemic rabbits (M-WHHL) were fed either standard rabbit diet or diet supplemented with 0.5% wt/wt of the lipophilic antioxidant vitamin E (d,l-alpha-tocopherol). Animals of 10-12 weeks of age were divided into two groups matched for distribution of serum cholesterol levels at the beginning of the 12 week study period. A significant hypocholesterolemic response to vitamin E feeding was observed throughout the study. Vitamin E supplementation increased serum vitamin E levels approximately fourfold and restricted ex-vivo copper mediated oxidative modification of low density lipoprotein (LDL) as quantitated by fluorescence at 430 nm. Post mortem examination of aortic tissue revealed a significant (32%) inhibition of surface area lesion involvement in the arch region as determined by image analysis. It is concluded that administration of vitamin E to M-WHHL rabbits brings about a significant hypocholesterolemic response, confers on LDL significant protection against oxidative modification and either or both contribute to the inhibition of early aortic lesion development.
Williams
R J
RJ
Department of Biochemistry, Glaxo Group Research, Middlesex, UK.
Motteram
J M
JM
Sharp
C H
CH
Gallagher
P J
PJ
D000818:Animals; D001011:Aorta / Q000473:pathology*; D002784:Cholesterol / Q000097:blood; D003300:Copper / Q000494:pharmacology; D004032:Diet; D006949:Hyperlipidemias / Q000097:blood / Q000473:pathology*; D008055:Lipids / Q000097:blood; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D011817:Rabbits; D014810:Vitamin E / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*
D008055:Lipids; D008077:Lipoproteins, LDL; D014810:Vitamin E; D003300:Copper; D002784:Cholesterol