Automated high-performance liquid chromatographic method for the determination of rifampicin in plasma.
Due to the unstable nature of rifampicin, a rapid automated high-performance liquid chromatographic method had to be developed for the analysis of a large number of plasma samples generated during a bioavailability trial. Extraction and injection of the samples were automatically done by a sample preparation system using C2, 100 mg Bond Elut extraction columns. The extracts were chromatographed on a 4-microns reversed-phase C18 column with a citrate buffer and acetonitrile as mobile phase. The analytes were detected at 342 nm. Calibration curves were linear to at least 20 micrograms/ml and the limit of quantification was 0.16 micrograms/ml.
Swart
K J
KJ
Department of Pharmacology, Faculty of Medicine, UOFS, Bloemfontein, South Africa.
Papgis
M
M
D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012293:Rifampin / Q000097:blood* / Q000493:pharmacokinetics
D012293:Rifampin