Long-term cultured mouse mast cells: ultrastructure, histamine and leukotriene levels.
Interleukin 3 (IL3), dependent cells were obtained from bone marrow (9/10 experiments) and spleen cells (4/5 experiments), but not from the thymus. These cells were similar to mucosal mast cell toluidine blue staining and electron microscopy. They had heterogenous metachromatic granules, and some had large scroll-like structures. They also contained histamine (200-800 ng/10(6) cells) for the first 2-5 weeks, whose level diminished to less than 30 ng/10(6) cells by 10 weeks of culture. They also generated leukotriene (LT) C4/D4 (10-40 ng/10(6) cells) and LTB4 (2-5 ng/10(6) cells) for over 100 days of culture. In one experiment, bone marrow-derived mast cells after 150 days of culture began to produce an IL3-like substance and proliferated exponentially without exogenous IL3.
Haisa
S
S
Allergic Disease Center, Creighton University, Omaha, Nebr.
Chang
H S
HS
Bewtra
A K
AK
Hiratani
M
M
Tamura
N
N
Bewtra
C
C
Townley
R G
RG
D000818:Animals; D001854:Bone Marrow Cells; D000001:Calcimycin / Q000494:pharmacology; D015550:Cell Degranulation; D002478:Cells, Cultured; D005260:Female; D006632:Histamine / Q000378:metabolism*; D066298:In Vitro Techniques; D015289:Leukotrienes / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism* / Q000648:ultrastructure*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology; D013997:Time Factors
D015289:Leukotrienes; D000001:Calcimycin; D006632:Histamine