title: A barley cDNA clone encoding a type III chlorophyll a/b-binding polypeptide of the light-harvesting complex II.
abstract: The nucleotide sequence of a leaf cDNA clone encoding a Type III chlorophyll a/b-binding (CAB) protein of light-harvesting complex II (LHCII) in barley is reported. Sequence comparisons and results from in vitro import into chloroplasts demonstrate that the cDNA clone encodes a functional transit peptide of 45 amino acid residues and a mature polypeptide of 223 residues with a predicted molecular mass of 24.3 kDa. After insertion into thylakoids, the mature protein is resistant to protease attack. Hybridization analysis using a gene-specific probe shows that the gene is expressed in dark-grown seedlings and that the amount of mRNA increases during illumination.
authors.affiliation: Department of Plant Biology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Brandt
authors.forename: V S
authors.initials: VS
authors.lastname: Nielsen
authors.forename: H
authors.initials: H
authors.lastname: Thordal-Christensen
authors.forename: D J
authors.initials: DJ
authors.lastname: Simpson
authors.forename: J S
authors.initials: JS
authors.lastname: Okkels
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA; D001467:Hordeum / Q000235:genetics*; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011498:Protein Precursors; D016415:Sequence Alignment
chemical_list: D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011498:Protein Precursors; C075576:CAB-3 protein, Hordeum vulgare; D004247:DNA