Hydrogen bond network of cytochrome P-450cam: a network connecting the heme group with helix K.
During investigations of the structural character of a mutant P-450cam where Glu-286 is replaced with lysine, we obtained evidence of a hydrogen bond network between helix K and the heme group via helix L of P-450cam. This mutant protein loses the ability to maintain the heme group in a proper position, possibly due to a break in the hydrogen bond network.
Yoshikawa
K
K
Department of Biology, Faculty of Science, Nagoya University, Japan.
Noguti
T
T
Tsujimura
M
M
Koga
H
H
Yasukochi
T
T
Horiuchi
T
T
Go
M
M
D001120:Arginine; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics; D006418:Heme / Q000737:chemistry*; D006860:Hydrogen Bonding; D008239:Lysine; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation
D006418:Heme; D003577:Cytochrome P-450 Enzyme System; D001120:Arginine; D008239:Lysine