title: Glomerulonephritis induced by murine chronic graft-versus-host reaction.
abstract: Severe glomerulonephritis was induced successfully in (B10 x DBA/2)F1 (BDF1) mice by injection of parental DBA/2 lymphoid cells. The mice manifested typical nephrotic syndrome dying around 10 weeks post injection. Electron microscopical examination demonstrated electron dense deposits first in the mesangial matrix, then in the subepithelium compatible with immune complex glomerulonephritis. Subendothelial deposits were not observed. Immunofluorescent study revealed IgG deposition in the capillary wall and IgM in the mesangium early in the process. As the lesion progressed, both IgG and IgM were present in the mesangial area and along the capillary wall. Some glomeruli showed segmental mesangiolysis, suggesting that altered mesangial cells have a role in the development of glomerular change, which together with rise in serum anti-DNA antibody titer suggest that autoantibodies promote the glomerular lesions in this model system.
authors.affiliation: Department of Pathology, Yokohama City University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Otani
authors.forename: I
authors.initials: I
authors.lastname: Aoki
authors.forename: Y
authors.initials: Y
authors.lastname: Miyagi
authors.forename: K
authors.initials: K
authors.lastname: Misugi
authors.forename: A
authors.initials: A
authors.lastname: Aoki
authors.forename: E
authors.initials: E
authors.lastname: Hagiwara
authors.forename: K
authors.initials: K
authors.lastname: Tani
authors.forename: Y
authors.initials: Y
authors.lastname: Ishigatsubo
authors.forename: T
authors.initials: T
authors.lastname: Okubo
mesh_terms: D000818:Animals; D005260:Female; D005921:Glomerulonephritis / Q000276:immunology / Q000473:pathology*; D006087:Graft vs Host Reaction; D007075:Immunoglobulin M / Q000032:analysis; D007678:Kidney Glomerulus / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008811:Mice, Inbred DBA; D008853:Microscopy; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence
chemical_list: D007075:Immunoglobulin M