Direct analysis of whole blood by internal surface reversed-phase chromatography: an examination of the binding and metabolism of technetium dioxime complexes.
We have developed a method using internal surface reversed-phase (ISRP) packing for rapid on-line separation of small hydrophobic compounds from cellular whole blood components. This is achieved by the use of 75-microns ISRP chromatographic material packed into a small high-performance liquid chromatographic (HPLC) column, in conjunction with column switching. We have applied this analytical method to study the in vitro metabolism of 99mTc-BATO (boronic acid adducts of technetium dioxime) cerebral and myocardial perfusion tracers in whole blood. The results from the ISRP procedure were compared with a conventional centrifugation method of analysis. This novel HPLC methods provides a rapid, convenient and reliable method for the analysis of radioactive and non-radioactive lipophilic components in whole blood.
Rosenspire
K C
KC
Bristol-Myers Squibb Pharmaceutical Research Institute, New Brunswick, NJ 08903.
Hirth
W
W
Jurisson
S
S
Nowotnik
D P
DP
Eckelman
W C
WC
Nunn
A D
AD
D000818:Animals; D001774:Blood Chemical Analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008297:Male; D015609:Organotechnetium Compounds; D010091:Oximes; D051381:Rats; D011919:Rats, Inbred Strains
D015609:Organotechnetium Compounds; D010091:Oximes