Magnetic bead purification of M13 DNA sequencing templates.
A method for the preparation of multiple M13 DNA sequencing templates is described. No phenol extraction is required and the procedure can be carried out in Eppendorf tubes or 96-well microtiter plates. Starting with a phage supernatant, the entire procedure is carried out in the same reaction vessel and all separation steps are based on a novel application of magnetic bead separation. The design of a 96-well magnetic separator is presented and the application of the method for large-scale sequencing is discussed.
Alderton
R P
RP
Imperial Cancer Research Fund, London, England.
Eccleston
L M
LM
Howe
R P
RP
Read
C A
CA
Reeve
M A
MA
Beck
S
S
D001435:Bacteriophages / Q000235:genetics; D004279:DNA, Viral / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D008280:Magnetics; D008863:Microspheres; D013698:Templates, Genetic
D004279:DNA, Viral