title: Release of endothelin in the oleic acid-induced respiratory distress syndrome in rats.
abstract: Rats injected intravenously with oleic acid developed pulmonary edema leading to hypoxia and hypercarbia. These changes were accompanied by an increase in immunoreactive endothelin (ir-ET) in plasma as early as 15 min after injection. At 45 min after injection plasma levels peaked at 114 +/- 19 pg/ml plasma (n = 8) and reached basal levels again after 240 min. In contrast, much larger amounts of ir-ET were found in the bronchoalveolar lavage fluid, with a peak at 120 min (2878 +/- 258 pg/lung, n = 7) preceding the maximum hypoxia observed at 180 min. In both plasma and bronchoalveolar lavage fluid samples ir-ET was characterized by reverse-phase HPLC as a mixture consisting mainly of ET-1 and smaller amounts of big ET-1, ET-2 and ET-3. In light of the biological effects of ET, the data suggest that these peptides might be of pathophysiological significance in this model of adult respiratory distress syndrome.
authors.affiliation: Department of Pharmacology and Toxicology, Ruhr-University, Bochum, F.R.G.
authors.forename: T
authors.initials: T
authors.lastname: Simmet
authors.forename: S
authors.initials: S
authors.lastname: Pritze
authors.forename: K I
authors.initials: KI
authors.lastname: Thelen
authors.forename: B A
authors.initials: BA
authors.lastname: Peskar
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004195:Disease Models, Animal; D016232:Endothelins / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D012710:Serum Albumin, Bovine; D013997:Time Factors
chemical_list: D016232:Endothelins; D009829:Oleic Acids; D012710:Serum Albumin, Bovine; D019301:Oleic Acid