The oligosaccharides in a recombinant hepatitis B virus surface antigen (HBsAg) carrying the pre-S2 region derived from yeast.
The N- and O-linked oligosaccharides in a yeast-derived HBsAg M protein (pre-S2 + S) were analyzed. Two major structures of the N-linked oligosaccharides bound to residue Asn4 were determined to be high-mannose type oligosaccharides, Man7GlcNAc2 and Man8GlcNAc2, by two-dimensional sugar mapping of the corresponding pyridylamino oligosaccharides. Peptide mapping of the M protein, sequence analysis of the glycopeptides after beta-elimination under reducing conditions and sugar-composition analysis revealed that the O-linked oligosaccharides were composed solely of mannose and bound to residue Ser5, Thr6, Thr7, Ser27, Ser28, Ser29 and Thr31 in the pre-S2 region.
Kobayashi
M
M
Biotechnology Research Laboratories, Tadeka Chemical Industries, Ltd., Osaka, Japan.
Ban
J
J
Asano
T
T
Utsunomiya
M
M
Kusumoto
S
S
Nishi
K
K
Kato
K
K
D000595:Amino Acid Sequence; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000737:chemistry; D008358:Mannose / Q000032:analysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism
D006514:Hepatitis B Surface Antigens; D009844:Oligosaccharides; D011994:Recombinant Proteins; D008358:Mannose