title: Outer membrane components of Campylobacter hyointestinalis.
abstract: Outer membranes were isolated, by sodium lauryl sulphate extraction, from the American type strain, five Australian, and four English isolates of Campylobacter hyointestinalis. On SDS-PAGE examination, the protein profiles of seven strains (including the type strain) were similar, and were dominated by two major proteins of 47 and 50 kDa. Three other isolates had unique major protein profiles. The largest of these proteins was heat-modifiable in these isolates, and in the type strain. The flagellin of three isolates screened was of similar M(r) to that of Campylobacter jejuni/Campylobacter coli. The lipopolysaccharides of C. hyointestinalis isolates were heterogeneous in structure; 5/10 isolates synthesised material of M(r) value greater than that of the low M(r) C. jejuni/C. coli lipopolysaccharide. By gel excision and re-electrophoresis, it was shown that the higher M(r) materials of one isolate were not artifactual aggregates of lower M(r) species.
authors.affiliation: Department of Microbiology, University of Adelaide, Australia.
authors.forename: S R
authors.initials: SR
authors.lastname: Waterman
authors.forename: J
authors.initials: J
authors.lastname: Hackett
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D002167:Campylobacter / Q000737:chemistry*; D005408:Flagellin / Q000302:isolation & purification*; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; D005408:Flagellin