title: Changes in cell-mediated immune responses after experimentally-induced anaphylaxis in dogs.
abstract: Experimentally-induced type 1 hypersensitivities were induced in normal dogs to either ovalbumin or Ascaris antigen. In vitro and in vivo cell-mediated immune responses were measured before sensitization and again at 1 and 6 days after induction of anaphylaxis by intravenous challenge with antigen. Histamine-modulated lymphocyte functions, such as histamine-induced suppression, histamine co-mitogen induced blastogenesis and the in vivo cutaneous responses to intradermally injected mitogens decreased post anaphylaxis. Spontaneous suppression of the autologous mixed-lymphocyte reaction increased post anaphylaxis. Lymphocyte blastogenic response to Concanavalin A (Con A) decreased at 6 (but not at 1) days post anaphylaxis probably due to a mediator other than histamine. Blastogenesis of 24 h preincubated cells by suboptimal concentration of Con A, declined post anaphylaxis, but Con A-induced suppression was not significantly altered. Dogs with atopic dermatitis have some altered cell-mediated immune responses. Altered histamine-induced and spontaneous suppression, histamine suppression of mitogenesis and decreased contact sensitivity observed in this experimental type 1 hypersensitivity mimicked that of atopic dogs. Increased cutaneous response to mitogens observed in atopic dogs was not reproduced in the type 1 hypersensitive dogs. These findings suggest some of the altered cell-mediated immune functions observed in dogs with atopic dermatitis result from type 1 hypersensitivity. The other abnormalities may be intrinsic to the atopic state.
authors.affiliation: Department of Pathology, Ontario Veterinary College, University of Guelph, Canada.
authors.forename: J S
authors.initials: JS
authors.lastname: Nimmo Wilkie
authors.forename: J A
authors.initials: JA
authors.lastname: Yager
authors.forename: B N
authors.initials: BN
authors.lastname: Wilkie
authors.forename: P J
authors.initials: PJ
authors.lastname: Pascoe
mesh_terms: D000707:Anaphylaxis / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D001200:Ascaris / Q000276:immunology; D003208:Concanavalin A; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004357:Drug Synergism; D005260:Female; D006632:Histamine; D007108:Immune Tolerance; D007111:Immunity, Cellular; D007114:Immunization / Q000662:veterinary; D007428:Intradermal Tests / Q000662:veterinary; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D010047:Ovalbumin / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D003208:Concanavalin A; D006632:Histamine; D010047:Ovalbumin