[Effect of vasopressin on phospholipid metabolism in cultured rat mesangial cells].
Aqueous solutions containing extracts of cultured rat mesangial cells treated with and without arginine vasopressin (AVP) were measured by phosphorus-31 nuclear magnetic resonance spectroscopy (31P-NMR). Ratios of the NMR signal intensities of phospholipids in the solutions were calculated with the areas of those resonance lines. Furthermore, prostaglandin E2 (PGE2) production and incorporation of 3H-methyl group into phospholipids in mesangial cells were observed in the presence and in the absence of AVP. We examined the role of AVP in the phospholipid metabolism of mesangial cells by the results from these three kinds of experiments. Ratios of (phosphorylcholine + glycerophosphorylcholine)/(phosphorylethanolamine + glycerophosphorylethanolamine), (PC + GPC)/(PE + GPE), and PC/PE increased significantly 5 min after AVP administration. Ratios of (GPE + GPC)/(PE + PC) and GPE/PE also increased significantly 30 min and 60 min after administration of AVP. The production of PGE2 increased with AVP treatment. This increment was inhibited with mepacrine and Adenosyl-S-isobutyl mercaptan (SIBA). Incorporation of 3H-methyl group into cellular phospholipids was accelerated with AVP, and then the resolution of methylated phospholipids and the release of PGE2 increased. These findings indicate that phospholipid methylation may be accelerated with AVP treatment and phosphatidylcholine, synthesized by transmethylation pathway, may be important as the substrate for PGE2 production in mesangial cells.
Nishida
M
M
Department of Pediatrics, Kyoto Prefectural University of Medicine, Japan.
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002478:Cells, Cultured; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
D010743:Phospholipids; D001127:Arginine Vasopressin