title: Vaccination of dogs against Babesia canis infection using parasite antigens from in vitro culture.
abstract: Groups of five dogs were vaccinated with different Babesia canis vaccine formulations. It appeared that partial protection against challenge infection was obtained when using parasite antigens from in vitro culture in combination with saponin. Protection was evident as a decrease in parasitaemia after challenge and was associated with the presence of serum antibodies against Babesia parasites. In addition, parasite antigen derived from in vitro culture supernatant exhibited more protective activity than somatic parasite antigen, in that a less marked fall of haematocrit values was found after challenge infection. The fall of haematocrit value observed in the animals immunized with somatic parasite antigen was not different from that observed in the adjuvant control group.
authors.affiliation: Department of Parasitology, Intervet International BV, Boxmeer, The Netherlands.
authors.forename: T P
authors.initials: TP
authors.lastname: Schetters
authors.forename: J
authors.initials: J
authors.lastname: Kleuskens
authors.forename: N
authors.initials: N
authors.lastname: Scholtes
authors.forename: H J
authors.initials: HJ
authors.lastname: Bos
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000008:administration & dosage* / Q000302:isolation & purification; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D001831:Body Temperature; D004285:Dogs; D006400:Hematocrit; D008297:Male; D012503:Saponins / Q000008:administration & dosage; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage / Q000302:isolation & purification
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D012503:Saponins; D014612:Vaccines