title: Modulation by the insecticides heptachlor and chlordane of the cell-mediated immune proliferative responses of rhesus monkeys.
abstract: Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood samples from twelve young, healthy male rhesus monkeys. Triplicate cultures were made in RPMI 1640 with 10% heat-inactivated homologous plasma with or without the test mitogens (phytohemagglutinin, concanavalin A and pokeweed mitogen). Under a defined assay condition, although a different monkey responded to a different degree (3 to 4-fold difference in stimulation index) to a specific mitogen, the proliferative responses of all monkeys were affected significantly by the addition of the chlorinated hydrocarbon heptachlor or chlordane to the test cultures. The insecticides, at 10-40 microM, may also act as mitogens for the unstimulated monkey PBMC or stimulate the release of IL-2 from a mitogen-stimulated culture. At 80 microM, both heptachlor and chlordane completely suppressed the proliferation and IL-2 release of the monkey lymphocytes. These studies suggest immunomodulatory effects of the insecticides heptachlor and chlordane.
authors.affiliation: Department of Medical Pharmacology and Toxicology, University of California, Davis 95616.
authors.forename: L F
authors.initials: LF
authors.lastname: Chuang
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: K
authors.initials: K
authors.lastname: Killam
authors.forename: R Y
authors.initials: RY
authors.lastname: Chuang
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002706:Chlordan / Q000494:pharmacology*; D003864:Depression, Chemical; D004347:Drug Interactions; D006533:Heptachlor / Q000494:pharmacology*; D007111:Immunity, Cellular / Q000187:drug effects*; D007376:Interleukin-2 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008253:Macaca mulatta / Q000276:immunology; D008297:Male; D008934:Mitogens
chemical_list: D007376:Interleukin-2; D008934:Mitogens; D002706:Chlordan; D006533:Heptachlor