title: Effect of progesterone in vitro on luteinizing hormone production in hen pituitary cells pretreated with estrogen.
abstract: Isolated cells from the pituitary gland of nonlaying hens were preincubated in vitro with or without estradiol-17 beta (E2), and then incubated with or without progesterone (P4), and luteinizing hormone (LH) in the cells and media was measured by a homologous radioimmunoassay. When the cells were preincubated with E2 for 2 h and then incubated with P4 for 4 h, cellular LH was increased, but LH in the medium remained unchanged. The increase in cellular LH in response to P4 was dose-dependent. Protein synthesis inhibitors (cycloheximide, actinomycin-D, and alpha-amanitin) blocked the response of the cells to P4. The results suggest that LH production in estrogen-primed pituitary cells of the hen was stimulated by P4 through a protein-synthesizing pathway.
authors.affiliation: Department of Poultry and Animal Sciences, Gifu University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawashima
authors.forename: A
authors.initials: A
authors.lastname: Ukai
authors.forename: M
authors.initials: M
authors.lastname: Kamiyoshi
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000502:physiology*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D007986:Luteinizing Hormone / Q000096:biosynthesis*; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011374:Progesterone / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D004967:Estrogens; D011374:Progesterone; D007986:Luteinizing Hormone