Effects of selected secondary metabolites of Fusarium moniliforme on unscheduled synthesis of DNA by rat primary hepatocytes.
The Fusarium moniliforme mycotoxins--fusarin C, fumonisin B1, moniliformin and bikaverin--were evaluated for genotoxicity by their ability to induce unscheduled DNA synthesis (UDS) in primary rat hepatocytes. Isolated hepatocytes were exposed to several concentrations of moniliformin (5.0-500 microM), bikaverin (1.0-500 microM), fumonisin B1 (0.5-250 microM), or fusarin C (1.0-100 microM). Aflatoxin B1, a known inducer of UDS, was included as a positive control. UDS was determined by autoradiography of cells after their exposure to [3H]thymidine. The highest doses of fusarin C and bikaverin caused cell death, but no cytotoxicity was observed in cells exposed to moniliformin or fumonisin B1. Fumonisin B1, moniliformin and bikaverin were not genotoxic in the UDS assay. The results of the UDS assay with fusarin C were inconclusive since a marginal effect on UDS was obtained.
Norred
W P
WP
Toxicology and Mycotoxin Research Unit, Richard B. Russell Agricultural Research Center, USDA/ARS, Athens, GA.
Plattner
R D
RD
Vesonder
R F
RF
Bacon
C W
CW
Voss
K A
KA
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D046508:Culture Techniques; D003503:Cyclobutanes / Q000633:toxicity; D004247:DNA / Q000096:biosynthesis*; D037341:Fumonisins; D005670:Fusarium / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009153:Mutagens / Q000633:toxicity; D009183:Mycotoxins / Q000633:toxicity*; D011090:Polyenes / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains; D014966:Xanthenes / Q000633:toxicity; D044004:Xanthones
D000970:Antineoplastic Agents; D003503:Cyclobutanes; D037341:Fumonisins; D009153:Mutagens; D009183:Mycotoxins; D011090:Polyenes; D014966:Xanthenes; D044004:Xanthones; C008539:moniliformin; C000269:bikaverin; C056933:fumonisin B1; D004247:DNA; C041403:fusarin C