Monitoring by two-dimensional electrophoresis somatic embryogenesis in leaf and petiole explants from Vitis.
A two-dimensional electrophoretic analysis of the total proteins was carried out in Vitis rupestris as model system in order to characterize the different developmental stages--from callus to plantlets--of somatic embryogenesis events in the grapevine. The patterns of callus, embryogenetic callus, somatic embryos and plantlets derived from leaf and petiole explants were compared. Each differentiation step was characterized by specific peptide spots.
Gianazza
E
E
Dipartimento di Scienze e Tecnologie Biomediche, Università di Milano, Italy.
De Ponti
P
P
Scienza
A
A
Villa
P
P
Martinelli
L
L
D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005638:Fruit / Q000737:chemistry* / Q000196:embryology; D006863:Hydrogen-Ion Concentration; D010940:Plant Proteins / Q000032:analysis*
D010940:Plant Proteins