Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila.
We describe an efficient method for generating female germline mosaics by inducing site-specific homologous mitotic recombination with a yeast recombinase (FLP) which is driven by a heat shock promoter. These germline mosaics are produced in flies heterozygous for the agametic, germline-dependent, dominant female sterile (DFS) mutation ovoD1, where only flies possessing germline clones are able to lay eggs. This method, the "FLP-DFS" technique, is very efficient because more than 90% of females with germline clones can be recovered. We show that this heat-inducible, site-specific mitotic recombination system does not affect viability and that the germline clones recovered are physiologically the same as those created by X-ray induced mitotic recombination. We describe the parameters of FLP-recombinase induced germline mitotic recombination and the use of the "FLP-DFS" technique to analyze the maternal effect of X-linked zygotic lethal mutations.
Chou
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TB
Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Perrimon
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D000818:Animals; D002678:Chimera; D002875:Chromosomes; D003433:Crosses, Genetic; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics*; D005260:Female; D005804:Genes, Lethal; D008040:Genetic Linkage; D005854:Germ Cells / Q000166:cytology* / Q000528:radiation effects; D006358:Hot Temperature; D008938:Mitosis; D016297:Mutagenesis, Site-Directed; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014960:X Chromosome
D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase