title: Proliferative response of mammary gland mononuclear cells to recombinant bovine interleukin-2.
abstract: Methods of augmenting bovine mononuclear cell responsiveness during physiological transitions of the udder may enhance resistance of the mammary gland to intramammary infections. Interleukin-2 is required for proliferation of T-lymphocytes and may contribute to B-lymphocyte proliferation. Recombinant bovine interleukin-2 (rBoIL-2) was evaluated as a potential immunoenhancer of bovine mammary gland mononuclear cells. Bovine mononuclear cells were isolated from five primiparous Holstein cows at 14-18 and 28-32 days of involution and at 7-13 days prior to parturition. Bovine blood and mammary gland mononuclear cells were highly responsive to rBoIL-2. Response of mammary gland mononuclear cells to rBoIL-2 was comparable with response of blood mononuclear cells. These data suggest that rBoIL-2 may be an effective immunoenhancer of bovine mononuclear cells during the non-lactating and prepartum periods.
authors.affiliation: Department of Animal Science, University of Tennessee, Knoxville 37901-1071.
authors.forename: P M
authors.initials: PM
authors.lastname: Torre
authors.forename: P K
authors.initials: PK
authors.lastname: Konur
authors.forename: S P
authors.initials: SP
authors.lastname: Oliver
mesh_terms: D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000276:immunology; D005260:Female; D007376:Interleukin-2 / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D007376:Interleukin-2; D011994:Recombinant Proteins