Inhibitory effect of demoxepam on tryptophan binding to rat hepatic nuclei.
Since some patients with eosinophilia-myalgia syndrome ingested tryptophan along with benzodiazepines, we investigated whether demoxepam, the N-desalkylated compound of chlordiazepoxide, would influence the binding of tryptophan to hepatic nuclei. L-Tryptophan has been shown to bind (saturable, stereospecific, and of high affinity) to rat hepatic nuclei and nuclear envelopes. We report that demoxepam has an inhibitory effect on in vitro [3H]tryptophan binding to rat hepatic nuclei and has an apparent KD approximately 22 microM.
Sidransky
H
H
Department of Pathology, George Washington University Medical Center, Washington, D.C. 20037.
Verney
E
E
Cosgrove
J W
JW
Schwartz
A M
AM
D000465:Algorithms; D000818:Animals; D014151:Anti-Anxiety Agents; D001569:Benzodiazepines; D001570:Benzodiazepinones / Q000494:pharmacology*; D002467:Cell Nucleus / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D014364:Tryptophan / Q000378:metabolism*
D014151:Anti-Anxiety Agents; D001570:Benzodiazepinones; D014316:Tritium; D001569:Benzodiazepines; D014364:Tryptophan; C000869:demoxepam