title: Identification of a protein-binding sequence involved in expression of an esterase gene from Streptomyces scabies.
abstract: Expression of an esterase gene from Streptomyces scabies is regulated by zinc in both Streptomyces scabies and Streptomyces lividans. A specific protein-binding site was identified on an esterase promoter fragment by using an S-30 extract from S. scabies. The location of the protein-binding site was determined by gel shift assays of promoter deletion fragments and by DNase I footprinting analysis. The protein-binding site maps from nucleotides -59 to -81 relative to the start of transcription. An esterase gene construct cloned and expressed in S. lividans was used to assess the importance of the protein-binding site. Deletion of the 23-bp protein-binding site resulted in a 10-fold decrease in esterase production when cells were grown in zinc-inducing conditions. The protein-binding site may represent a region involved in positive regulation of the S. scabies esterase gene.
authors.affiliation: Department of Biochemistry, University of Minnesota, St. Paul 55108.
authors.forename: M J
authors.initials: MJ
authors.lastname: Babcock
authors.forename: M
authors.initials: M
authors.lastname: McGrew
authors.forename: J L
authors.initials: JL
authors.lastname: Schottel
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004950:Esterases / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D011485:Protein Binding; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D004950:Esterases; D003850:Deoxyribonuclease I