Cytoprotection by 16,16-dimethylprostaglandin E2. Role of gastric juice and mucus gel layer.
A mechanism of cytoprotection by 16,16-dimethylprostaglandin E2 (16,16-dimethyl-PGE2) was investigated in rats with respect to possible roles of the gastric juice and the mucus gel layer. 16,16-Dimethyl-PGE2 significantly increased the volume of gastric juice and mucus thickness. In a gastric juice-emptied stomach, 16,16-dimethyl-PGE2 protected the gastric mucosa against 30% ethanol, but not against 40% ethanol. Moreover, this agent did not show any cytoprotective action even against 30% ethanol when both gastric juice and mucus gel were removed. In this situation there was no histological change in the depth of injury, whether 16,16-dimethyl-PGE2 was given as pretreatment or not. These results suggest that there are two mechanisms that may play major roles in the cytoprotection of the gastric mucosa by 16,16-dimethyl-PGE2: (a) dilution of necrotizing agents by increased gastric juice and (b) thickening of the mucus gel layer.
Taniguchi
Y
Y
Department of Gastroenterology, Jichi Medical School, Tochigi, Japan.
Yoshida
Y
Y
Kimura
K
K
Mato
M
M
D000818:Animals; D000431:Ethanol / Q000008:administration & dosage / Q000009:adverse effects; D005750:Gastric Juice / Q000187:drug effects* / Q000502:physiology; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D009336:Necrosis; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D011459:Prostaglandins E, Synthetic; D000431:Ethanol; C017244:16,16-dimethylprostaglandin E