Immunocytochemical localization of VIP-immunoreactive neurons in the hypothalamus of immature gilts.
Immunocytochemical study using avidin-biotin complex method and peroxidase-antiperoxidase technique was carried out to determine the distribution and morphology of vasoactive intestinal polypeptide-immunoreactive (VIP-IR) neuronal structures in the hypothalamus of the immature female pig. VIP-IR neurons were found in the arcuate, paraventricular and supraoptic nuclei, as well as in the pituitary stalk. The median eminence disclosed an abundance of VIP-IR processes both in the internal and external layer. These results let us conclude that there exist convenient morphological conditions for the release of VIP into portal blood in the area of the porcine median eminence-pituitary stalk complex.
Calka
J
J
Department of Animal Anatomy, Veterinary Faculty, Agricultural and Technical University of Olsztyn, Poland.
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000378:metabolism; D005260:Female; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008473:Median Eminence / Q000166:cytology / Q000378:metabolism; D010904:Pituitary Gland, Posterior / Q000166:cytology / Q000378:metabolism; D013552:Swine; D014660:Vasoactive Intestinal Peptide / Q000276:immunology / Q000378:metabolism*
D014660:Vasoactive Intestinal Peptide