title: [Cytochemical ultrastructural study of adenosine 5'monophosphate hydrolysis in human spermatozoa].
abstract: A specific 5'nucleotidase activity (pH 7.4) is localized by cytochemical technics, in the head (nuclear membrane, periacrosomal cytoplasm) and in the mitochondrial membrane of human spermatozoa. The effect of different effectors (manganese, nickel, fluoride, tartrate, tetramisole), studied in the head by rate assessment of cells with or without hydrolysis sites, changes the cellular distribution, the depth of reactions and their localization. The 5'nucleotidase interferences with some unspecific phosphatases are discussed.
authors.affiliation: Laboratoire de Biologie du Développement et de la Reproduction Humaine, Faculté de Médecine, Montpellier, France.
authors.forename: B
authors.initials: B
authors.lastname: Caporiccio
authors.forename: M
authors.initials: M
authors.lastname: Chalet
authors.forename: O
authors.initials: O
authors.lastname: Auziech
authors.forename: G
authors.initials: G
authors.lastname: Catayee
mesh_terms: D015720:5'-Nucleotidase / Q000032:analysis; D000249:Adenosine Monophosphate / Q000378:metabolism*; D000418:Albumins / Q000494:pharmacology; D006651:Histocytochemistry; D006801:Humans; D006868:Hydrolysis; D008297:Male; D008345:Manganese / Q000494:pharmacology; D009532:Nickel / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology; D013094:Spermatozoa / Q000201:enzymology* / Q000648:ultrastructure; D013644:Tartrates / Q000494:pharmacology; D013773:Tetramisole / Q000494:pharmacology
chemical_list: D000418:Albumins; D013644:Tartrates; D000249:Adenosine Monophosphate; D008345:Manganese; D009532:Nickel; D012969:Sodium Fluoride; D013773:Tetramisole; D015720:5'-Nucleotidase; C029768:tartaric acid
vernacular_title: Etude cytochimique ultrastructurale de l'hydrolyse de l'adenosine 5'monophosphate dans le spermatozoide humain.