An improved CPG support for the synthesis of 3'-amine-tailed oligonucleotides.
A new controlled-pore glass (CPG) support is described that allows for the direct synthesis of oligonucleotides bearing a 3'-aminohexyl tail. This solid support (AH-CPG) exhibits superior performance as compared to a commercially available 3'-amine CPG. The AH-CPG is prepared from 6-aminohexan-1-ol with a unique protecting group for the amine that also functions as the site of attachment to the CPG. A 3'-amine-tailed oligodeoxynucleotide (ODN) was prepared from this support using standard phosphoramidite coupling and deprotection conditions. The 3'-amine-tailed ODN was subsequently modified with an acridinylpropionic acid tetrafluorophenyl ester. Facile synthesis of the AH-CPG and the stability of the deprotected product makes this functionalized solid support especially useful for preparation of oligonucleotides bearing 3'-amine tails and other modifications.
Petrie
C R
CR
MicroProbe Corporation, Bothell, Washington 98021.
Reed
M W
MW
Adams
A D
AD
Meyer
R B
RB
D000166:Acridines / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005898:Glass; D009841:Oligonucleotides / Q000138:chemical synthesis*
D000166:Acridines; D009841:Oligonucleotides