Changes in growth hormone and prolactin messenger ribonucleic acid levels during seawater adaptation of amago salmon (Oncorhynchus rhodurus).
To examine the changes in secretion of growth hormone (GH) and prolactin (PRL) with reference to their osmoregulatory roles, changes in pituitary mRNA levels and plasma concentrations of these hormones were examined during seawater adaptation in silvery juveniles (smolts) and precociously mature males (dark parr) of amago salmon (Oncorhynchus rhodurus). Transfer to seawater increased plasma sodium levels in both smolts and dark parr. Smolts adjusted their plasma sodium to the level associated with seawater-adaptation (165 mEq/liter) within 3 days, whereas no adjustment was seen in dark parr; the latter failed to survive in seawater for more than 3 days. In smolts, plasma GH levels increased significantly 1 day after transfer, whereas there was no significant change in dark parr. An increase in GH mRNA levels was observed in smolts in association with increased plasma GH, whereas there was no change in dark parr. In contrast, a reduction in plasma PRL levels was consistently observed in both smolts and dark parr after transfer to seawater. However, there was no significant change in PRL mRNA levels in either smolts or dark parr. These results suggest that both gene expression and release of GH are activated by seawater transfer only in smolts with adequate seawater adaptability, whereas PRL gene expression is decreased after seawater transfer regardless of seawater adaptability.
Yada
T
T
Ocean Research Institute, University of Tokyo, Japan.
Kobayashi
T
T
Urano
A
A
Hirano
T
T
D000222:Adaptation, Physiological; D000818:Animals; D015152:Blotting, Northern; D005786:Gene Expression Regulation; D013006:Growth Hormone / Q000097:blood* / Q000235:genetics; D015345:Oligonucleotide Probes; D010902:Pituitary Gland / Q000378:metabolism; D011388:Prolactin / Q000097:blood* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D011863:Radioimmunoassay; D012474:Salmon; D012623:Seawater; D012964:Sodium / Q000097:blood
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011388:Prolactin; D013006:Growth Hormone; D012964:Sodium