Studies on the intracellular Ca2+, protein kinase activity, and ATP contents of cisplatin- and rIFN-Y-treated non-adherent mononuclear cells.
The in vitro effect of cisplatin or rIFN-Y on the intracellular free calcium, protein kinase activity and ATP content of non-adherent mononuclear cells (nMNC) from human peripheral blood was assessed. We observed that treatment of nMNC with cisplatin enhanced the [Ca2+]i, ATP level, and protein kinase activity; whereas rIFN-Y-treated nMNC showed significant rises in ATP level and protein kinase activity only. We also observed that cisplatin-treated nMNC showed an instantaneous rise in [Ca2+]i when NK-sensitive K562 cells were added to the effector cells.
Pai
K
K
Department of Zoology, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Sodhi
A
A
D000255:Adenosine Triphosphate / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D002945:Cisplatin / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology*; D007424:Intracellular Fluid / Q000378:metabolism; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000276:immunology
D007371:Interferon-gamma; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D002945:Cisplatin; D002118:Calcium