Determination of 4-aminopyridine in serum by solid-phase extraction and high-performance liquid chromatography.
An assay for the determination of 4-aminopyridine in serum has been developed using 3,4-diaminopyridine as internal standard and reversed-phase high-performance liquid chromatography with detection at 244 nm. A mobile phase of acetonitrile-methanol-ethanol-1% ammonium carbonate (75:10:10:5) provided excellent separation of both compounds. Samples were extracted on solid-phase columns. The linearity, precision, recovery and the limit of detection were all sufficient for the routine use of this assay in clinical studies of patients treated with 4-aminopyridine.
van der Horst
A
A
Department of Pharmacy, Free University Hospital, Amsterdam, Netherlands.
de Goede
P N
PN
van Diemen
H A
HA
Polman
C H
CH
Martens
H J
HJ
D015761:4-Aminopyridine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D006801:Humans; D010919:Placebos; D015203:Reproducibility of Results
D010919:Placebos; D015761:4-Aminopyridine