title: Long-term survival of mouse corpus callosum grafts in neonatal rat recipients, and the effect of host sensitization.
abstract: Previous studies have suggested that the incidence of spontaneous rejection among immunogenetically mismatched neural transplants in neonatal recipients varies significantly depending on the cellular composition of the graft material. For example, neuron-rich grafts of embryonic mouse retina generally survive for extended periods without showing signs of rejection after implantation into neonatal rats, whereas cortical xenografts, which contain abundant glial and endothelial cells as well as neurons, typically undergo rejection 4-6 weeks after implantation. To determine whether the presence of donor glia is responsible for this high incidence of spontaneous rejection, we examined the fate of a non-neuronal graft material composed predominantly of xenogeneic glial cells (post-natal day 3, PD3, CD-1 mouse corpus callosum) implanted into the mesencephalon of PD1 Sprague-Dawley rats. The distribution and survival of donor astrocytes were assessed using a monoclonal antibody specific for a mouse astrocyte surface antigen, M2. Thirteen of 16 animals sacrificed within 2 months of implantation had detectable transplants. In these animals, M2-positive cells frequently migrated well away from body of the graft, clustering in large numbers in several characteristic regions of the host brain. Unlike cortical grafts of similar age, the vast majority (93%) of callosal transplants showed no histological signs of rejection or major histocompatibility complex antigen expression in and around the transplant-derived cells. As previously noted in the neonatal retinal transplant paradigm, however, well-integrated 1-month-old corpus callosum grafts could be induced to reject by appropriate sensitization of the host immune system, implying that the host was not immunologically tolerant to the foreign neural graft. With longer survival times in unsensitized hosts, a progressively smaller percentage of animals had detectable donor astrocytes (5 of 10 animals at 3 months postimplantation and 4 of 16 animals at 4 months); in those 9 animals with surviving grafts, only small numbers of M2-positive cells were seen within the graft bed and surrounding host brain. However, only 2 of the 26 "long-term" animals showed evidence of graft rejection. These results indicate that mouse astrocytes show characteristic patterns of migration into the host brain when implanted into neonatal rats; however, these xenogeneic cells have a limited duration of survival. The infrequency with which even subtle signs of spontaneous rejection were detected in animals that had received corpus callosum xenografts suggests that an immune-mediated process is unlikely to be responsible for the time-dependent elimination of the donor astrocytes.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Department of Neurosurgery, University of Pittsburgh School of Medicine, PA 15261.
authors.forename: I F
authors.initials: IF
authors.lastname: Pollack
authors.forename: L H
authors.initials: LH
authors.lastname: Lee
authors.forename: H F
authors.initials: HF
authors.lastname: Zhou
authors.forename: R D
authors.initials: RD
authors.lastname: Lund
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis; D001253:Astrocytes / Q000276:immunology / Q000637:transplantation; D015415:Biomarkers; D016380:Brain Tissue Transplantation / Q000276:immunology*; D002465:Cell Movement; D003337:Corpus Callosum / Q000637:transplantation*; D015353:Eye Enucleation; D006084:Graft Rejection / Q000276:immunology; D006085:Graft Survival; D006649:Histocompatibility Antigens / Q000096:biosynthesis; D007114:Immunization; D008636:Mesencephalon; D051379:Mice / Q000276:immunology*; D009410:Nerve Degeneration / Q000276:immunology; D009900:Optic Nerve / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology*; D016038:Skin Transplantation / Q000276:immunology; D014183:Transplantation, Heterologous / Q000276:immunology*; D016042:Transplantation, Heterotopic
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D006649:Histocompatibility Antigens