A novel microbial sensor using luminous bacteria.
A novel microbial sensor system that uses luminous bacteria was developed for the determination of both glucose and toxic compounds. The sensor system consisted of a membrane with luminous bacteria immobilized upon it and a photomultiplier. Measurements were based on the in vivo intensity of the light emitted by the bacteria, as this is affected by their environment. A linear relationship was observed between increased luminescence and concentrations of glucose between 0.05 mM and 0.55 mM. The relative standard deviation was 10% for 0.55 mM glucose (n = 10). Toxic compounds such as benzalkonium chloride, sodium dodecyl sulphate and chromium(VI) were also detected by measuring the decrease in luminescence in their presence.
Lee
S
S
Research Centre for Advanced Science and Technology, University of Tokyo, Japan.
Sode
K
K
Nakanishi
K
K
Marty
J L
JL
Tamiya
E
E
Karube
I
I
D001419:Bacteria; D001548:Benzalkonium Compounds / Q000032:analysis; D015374:Biosensing Techniques; D002857:Chromium / Q000032:analysis; D005947:Glucose / Q000032:analysis; D008163:Luminescent Measurements; D012967:Sodium Dodecyl Sulfate / Q000032:analysis
D001548:Benzalkonium Compounds; D002857:Chromium; D012967:Sodium Dodecyl Sulfate; D005947:Glucose