title: A novel microbial sensor using luminous bacteria.
abstract: A novel microbial sensor system that uses luminous bacteria was developed for the determination of both glucose and toxic compounds. The sensor system consisted of a membrane with luminous bacteria immobilized upon it and a photomultiplier. Measurements were based on the in vivo intensity of the light emitted by the bacteria, as this is affected by their environment. A linear relationship was observed between increased luminescence and concentrations of glucose between 0.05 mM and 0.55 mM. The relative standard deviation was 10% for 0.55 mM glucose (n = 10). Toxic compounds such as benzalkonium chloride, sodium dodecyl sulphate and chromium(VI) were also detected by measuring the decrease in luminescence in their presence.
authors.affiliation: Research Centre for Advanced Science and Technology, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: K
authors.initials: K
authors.lastname: Sode
authors.forename: K
authors.initials: K
authors.lastname: Nakanishi
authors.forename: J L
authors.initials: JL
authors.lastname: Marty
authors.forename: E
authors.initials: E
authors.lastname: Tamiya
authors.forename: I
authors.initials: I
authors.lastname: Karube
mesh_terms: D001419:Bacteria; D001548:Benzalkonium Compounds / Q000032:analysis; D015374:Biosensing Techniques; D002857:Chromium / Q000032:analysis; D005947:Glucose / Q000032:analysis; D008163:Luminescent Measurements; D012967:Sodium Dodecyl Sulfate / Q000032:analysis
chemical_list: D001548:Benzalkonium Compounds; D002857:Chromium; D012967:Sodium Dodecyl Sulfate; D005947:Glucose