Effects of recombinant cytokines on murine megakaryocyte colony formation in a serum-free fibrin clot culture system.
A convenient serum-free fibrin clot culture system for murine megakaryocyte progenitor cells was developed. The culture and counting of colonies is much easier in this system, when compared with previously reported serum-free culture methods. Recombinant murine interleukin-3 (rmIL-3) stimulated megakaryocyte colony formation in a dose-dependent manner in this system. While recombinant human granulocyte colony-stimulating factor (rhG-CSF) had no effect on megakaryocytopoiesis, recombinant human erythropoietin (rhEpo) and recombinant human interleukin-6 (rhIL-6) augmented megakaryocyte colony formation stimulated by rmIL-3. The depletion of adherent cells and T cells from the cultured bone marrow did not eliminate the synergistic effect of rhEpo and rhIL-6.
Ogata
K
K
Hipple Cancer Research Center, Dayton, Ohio 45439-2092.
Erickson-Miller
C L
CL
Nomura
T
T
Abe
K
K
Zhang
Z
Z
Murphy
M J
MJ
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D016895:Culture Media, Serum-Free; D046508:Culture Techniques / Q000379:methods*; D016207:Cytokines / Q000494:pharmacology*; D005337:Fibrin; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D008533:Megakaryocytes / Q000187:drug effects*; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000502:physiology
D016895:Culture Media, Serum-Free; D016207:Cytokines; D016298:Hematopoietic Cell Growth Factors; D011994:Recombinant Proteins; D005337:Fibrin