Conserved signals in the 5' flanking region of eukaryotic nuclear tRNA genes.
The statistical analysis of 5' flanking regions of eukaryotic tRNA genes was done. The analysis of nucleotides in the sequence of fungi and invertebrates showed a high content of A and T in the flanking regions versus coding regions where G and C dominate. In contrast to these results in vertebrates sequences the preferences of any nucleotide in flanking regions was not observed. The analysis of tetrads showed five conserved signals: TTGT, (T/A)(T/A)ATA, A(C/T)(C/A)A in the tRNA genes of fungi, (A/T)TGA of invertebrates and (A/T)GAG of vertebrates. The analysis of 3' flanking regions did not show any conserved signals except well known poly-T tracks.
Makałowski
W
W
Poznań University, Department of Biopolymer Biochemistry, Poland.
Augustyniak
J
J
D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence / Q000235:genetics; D005057:Eukaryotic Cells; D005658:Fungi / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D007448:Invertebrates / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D014714:Vertebrates / Q000235:genetics
D012343:RNA, Transfer