Cross-reactivity of polyclonal serum antibodies generated against Cryptosporidium parvum oocysts.
Polyclonal antibodies raised against Cryptosporidium parvum oocysts were found to cross-react with Eimeria spp. oocyst antigens in an indirect immunofluorescence assay, and sera from Eimeria spp.-infected lambs reacted with some antigens from sonicated C. parvum oocysts (between 29 to 30 and 66 to 69 kDa) by Western blot (immunoblot). No cross-reaction was observed with cystozoites of Toxoplasma and Sarcocystis spp. These results show the existence of epitopes common to C. parvum and various Eimeria spp.
Ortega-Mora
L M
LM
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Troncoso
J M
JM
Rojo-Vázquez
F A
FA
Gómez-Bautista
M
M
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000032:analysis; D015153:Blotting, Western; D003429:Cross Reactions; D016785:Cryptosporidium parvum / Q000254:growth & development; D004539:Eimeria / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D011817:Rabbits; D012756:Sheep
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan