title: Determination of optimal in vitro conditions for caprine alternative complement pathway assay.
abstract: Optimal in vitro testing conditions for caprine alternative complement pathway assay were determined. Effects of the following variables were tested: heterologous erythrocytes; pH, ionic strength and Mg2+ ion concentration of the complement diluent; incubation time and temperature. Rabbit erythrocytes were the optimal target cells. The optimal buffer conditions were: pH 8.0, ionic strength 0.06 mmol NaCl and 5mmol Mg2+ ion. Optimal incubation time and temperature were 75 min and 30 degrees C, respectively.
authors.affiliation: Immunology Section, Indian Veterinary Research Institute, Izatnagar.
authors.forename: G
authors.initials: G
authors.lastname: Venugopal
authors.forename: G C
authors.initials: GC
authors.lastname: Ram
authors.forename: M P
authors.initials: MP
authors.lastname: Bansal
mesh_terms: D000818:Animals; D003176:Complement C3 / Q000032:analysis*; D015941:Complement Hemolytic Activity Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D003170:Complement Pathway, Alternative; D004912:Erythrocytes / Q000276:immunology; D006041:Goats; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D009994:Osmolar Concentration; D013696:Temperature; D013997:Time Factors
chemical_list: D003176:Complement C3; D008274:Magnesium