Modulation of 2',5'-oligoadenylate synthetase in patients treated with alpha-interferon: effects of dose, schedule, and route of administration.
The interferon (IFN)-induced intracellular enzyme 2',5'-oligoadenylate (2-5A) synthetase was measured in extracts of peripheral mononuclear cells isolated from patients receiving a 300-fold range of doses of alpha interferon (IFN-alpha). The range of enzyme induction was 2.3- to 5.7-fold. The maximum fold increase varied from individual to individual as did the dose required for maximum enzyme stimulation. The magnitude and endurance of the enzyme response was a function of IFN dose and was unrelated to the duration of treatment or number of injections or to the route of administration. The enzyme assay was a more sensitive indicator of IFN administration than was measurement of the level of circulating IFN. These results substantiate the potential of a clinical 2-5A synthetase assay for monitoring IFN treatment.
Merritt
J A
JA
Department of Human Oncology, University of Wisconsin-Madison 53706.
Ball
L A
LA
Sielaff
K M
KM
Meltzer
D M
DM
Borden
E C
EC
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis*; D001681:Biological Assay; D004305:Dose-Response Relationship, Drug; D004333:Drug Administration Routes; D004334:Drug Administration Schedule; D004341:Drug Evaluation; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D016898:Interferon-alpha / Q000032:analysis / Q000494:pharmacology*; D009369:Neoplasms / Q000201:enzymology / Q000628:therapy
D016898:Interferon-alpha; D015088:2',5'-Oligoadenylate Synthetase