title: Avidin-induced lysis of biotinylated erythrocytes by homologous complement via the alternative pathway depends on avidin's ability of multipoint binding with biotinylated membrane.
abstract: It was reported that avidin and streptavidin induce lysis of prebiotinylated red blood cells via the alternative pathway of both homologous and heterologous complement. Both of these proteins have four biotin-binding sites, providing a polyvalent interaction with biotinylated components of the erythrocyte membrane. We have compared the effects of mono- and multipoint avidin attachment on the sensitivity of biotinylated erythrocytes to lysis by the complement system. In the presence of anti-avidin antibody, avidin-bearing biotinylated erythrocytes were rapidly lysed by heterologous serum. This lysis was independent from the mode of avidin attachment, implying that complement activation by the classical pathway triggered by interaction between C1 and avidin-bound antibody on the erythrocyte surface is independent from the avidin's ability of polyvalent (multipoint) binding with biotinylated membrane components. In the absence of anti-avidin antibody, biotinylated erythrocytes bearing polyvalently attached avidin were lysed by homologous complement better than cells bearing avidin, which possesses reduced ability for multipoint binding with biotinylated erythrocyte. Two independent approaches to reduce avidin's ability of multipoint binding were used: decrease in surface density of biotin on the erythrocyte membrane and blockage of biotin-binding sites of avidin. Both methods result in reduced lysis of avidin-bearing erythrocytes as compared with erythrocytes bearing an equal amount of polyvalent-bound avidin. Thus the activation of homologous complement via the alternative pathway depends on avidin's ability to 'cross-link' to the biotinylated components of the erythrocyte membrane.
authors.affiliation: Institute of Experimental Cardiology, National Cardiology Research Center, Moscow, Russia.
authors.forename: V R
authors.initials: VR
authors.lastname: Muzykantov
authors.forename: M D
authors.initials: MD
authors.lastname: Smirnov
authors.forename: G P
authors.initials: GP
authors.lastname: Samokhin
mesh_terms: D000818:Animals; D001360:Avidin / Q000378:metabolism / Q000494:pharmacology*; D001710:Biotin / Q000378:metabolism; D015941:Complement Hemolytic Activity Assay; D003170:Complement Pathway, Alternative; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006168:Guinea Pigs; D006461:Hemolysis; D012756:Sheep
chemical_list: D001360:Avidin; D001710:Biotin