Microanalysis of beta-cyclodextrin and glucosyl-beta-cyclodextrin in human plasma by high-performance liquid chromatography with pulsed amperometric detection.
High-performance liquid chromatography with pulsed amperometric detection was applied to the determination of beta-cyclodextrin (beta-CD) and glucosyl (G)-beta-CD in human plasma. They were well resolved from each other and from background components of plasma on a polymer-based reversed-phase column with 0.6% acetonitrile aqueous solution containing 1 mM sodium hydroxide as an eluent. The samples in the effluent were detected with a pulsed amperometric detector after postcolumn alkalization. The detection limits of beta-CD and G-beta-CD in plasma at a signal-to-noise ratio of 3 were 11 and 5 pmol, respectively.
Kubota
Y
Y
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Fukuda
M
M
Ohtsuji
K
K
Koizumi
K
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003505:Cyclodextrins / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C031215:betadex