A modified colorimetric method for the determination of orthophosphate in the presence of high ATP concentrations.
We describe a modified colorimetric method that quantitates inorganic phosphate linearly up to 60 nmol, with high stability of the developed color and with a low interference by ATP concentration (up to 30 mM). This method is very suitable for use in ATPase enzymatic assays, especially with enzymes that have low specific activities and (or) high Km values for ATP.
González-Romo
P
P
Departamento de Bioquímica, DEPg, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City.
Sánchez-Nieto
S
S
Gavilanes-Ruíz
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D003124:Colorimetry / Q000379:methods; D007202:Indicators and Reagents; D008861:Microsomes / Q000378:metabolism; D010710:Phosphates / Q000032:analysis*; D003313:Zea mays / Q000378:metabolism
D007202:Indicators and Reagents; D010710:Phosphates; D000255:Adenosine Triphosphate