PCR libraries of ancient DNA using a generalized PCR method.
We describe a generalized PCR method that will amplify fragments of DNA without any knowledge of sequence using a single primer. Although we are presently using this method to amplify DNA fragments isolated from ancient preserved tissues, in effect, producing PCR libraries, it may prove to have other applications.
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Department of Biochemistry, University of Minnesota, Duluth 55812.
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Aufderheide
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D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D009106:Mummies; D016133:Polymerase Chain Reaction / Q000379:methods*
D004247:DNA