title: Detection of borreliacidal antibodies in hamsters by using flow cytometry.
abstract: Flow cytometry can be used to detect antibody that kills Borrelia burgdorferi. Borreliacidal activity was detected within 3 h of incubating B. burgdorferi with immune serum and complement. Right-angle light scatter and propidium iodide fluorescence were the cytometric parameters which correlated best with in vitro killing of B. burgdorferi. Flow cytometry is a rapid method for determining the presence of borreliacidal activity and may lead to a better serodiagnostic test for the detection of Lyme disease.
authors.affiliation: Wisconsin State Laboratory of Hygiene, Department of Medical Microbiology and Immunology, University of Wisconsin, Madison 53706.
authors.forename: K F
authors.initials: KF
authors.lastname: Sachsenmeier
authors.forename: K
authors.initials: K
authors.lastname: Schell
authors.forename: L W
authors.initials: LW
authors.lastname: Morrissey
authors.forename: D R
authors.initials: DR
authors.lastname: Pennell
authors.forename: R M
authors.initials: RM
authors.lastname: West
authors.forename: S M
authors.initials: SM
authors.lastname: Callister
authors.forename: R F
authors.initials: RF
authors.lastname: Schell
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D015748:Borrelia burgdorferi Group / Q000276:immunology* / Q000302:isolation & purification; D006224:Cricetinae; D005434:Flow Cytometry / Q000379:methods*
chemical_list: D000907:Antibodies, Bacterial