Specific identification of human ribonucleases by antibodies produced against two synthetic peptides corresponding to the N- and C-terminal amino-acid sequences of human urinary secretory-type ribonuclease.
Antibodies were raised in rabbits by immunizing two synthetic peptides, which corresponded to the N- and C-terminal 15 residues, respectively, of human urinary secretory ribonuclease (RNase). These antibodies did not block the RNase activity, but reacted well with enzymes blotted onto a transfer membrane following electrophoresis, and discriminated strictly between secretory- and nonsecretory-type RNases. Therefore, these antibodies should to be valuable tools for the immunological identification of human RNases.
Yasuda
T
T
Department of Legal Medicine, Fukui Medical School, Japan.
Nadano
D
D
Tanaka
Y
Y
Kishi
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits; D012260:Ribonucleases / Q000032:analysis / Q000276:immunology*
D000906:Antibodies; D010446:Peptide Fragments; D012260:Ribonucleases