Hydroxylation of 19-norandrogens by porcine Leydig cells.
The metabolism of 19-norandrogens by porcine Leydig cells was investigated. Non-radioactive 19-norandrostenedione (19-Nor A) and [3H]19-nortestosterone (19-Nor T) were used as substrates in incubations with cell preparations from mature male pigs. Steroid products were separated by reversed-phase HPLC and material in selected peaks was rechromatographed before attempts to identify them by GC-MS. Both 11 beta- and 6 beta-hydroxylated derivatives of 19-Nor A were found and a third product (11-oxo-19-Nor A) was tentatively identified. The profile of radioactive metabolites from [3H]19-Nor T also favours the view of a capacity for hydroxylation of 19-norandrogens by porcine Leydig cells. The significance of these findings together with our earlier report of direct 11 beta-hydroxylation of C19 steroids by such cells remains to be examined.
Raeside
J I
JI
Department of Biomedical Sciences, University of Guelph, Ontario, Canada.
Renaud
R L
RL
Marshall
D E
DE
D000735:Androstenedione / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006900:Hydroxylation; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D009277:Nandrolone / Q000378:metabolism*; D013552:Swine
D000735:Androstenedione; D009277:Nandrolone; C002828:19-norandrostenedione