PCR amplification of specific cDNA sequences using mixed oligonucleotide primers--a cautionary note.
O'Connell
J
J
Department of Biochemistry, University College, Cork, Ireland.
Doonan
S
S
D000363:Agaricales / Q000201:enzymology / Q000235:genetics; D000595:Amino Acid Sequence; D001219:Aspartate Aminotransferases / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*
D004271:DNA, Fungal; D009838:Oligodeoxyribonucleotides; D004247:DNA; D001219:Aspartate Aminotransferases