[Hydrolysis and absorption of lysozyme in the small intestine].
Preparations of chicken small intestine were used in the experiment in vitro simulating processes of membranous digestion (inverted intestinal segments) and absorption (inverted intestinal myasis). It was established that lysozyme was hydrolyzed on the internal mucosa surface regardless of its concentration in the gastro-intestinal tract, and only insignificant quantity of lysozyme (0.027%) penetrates the intestinal wall. The method of lysozyme determination through its action on the cellular wall of Micrococcus lisodeicticus, and highly efficient liquid chromatography were used to study the transport process. The data presented have evidenced that lysozyme is well hydrolyzed under the action of intestinal peptide hydrolyses, and only insignificant amounts of non-splitted lysozyme can penetrate the blood.
Basova
N A
NA
Mikelsone
V Ia
VIa
Tarvid
I L
IL
Kushak
R I
RI
Grigor'eva
V
V
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004531:Eggs; D006868:Hydrolysis; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000502:physiology*; D007421:Intestine, Small / Q000201:enzymology* / Q000502:physiology; D008954:Models, Biological; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*
D009113:Muramidase
Gidroliz i vsasyvanie lizotsima v tonkoĭ kishke.