Effect of progesterone in vitro on luteinizing hormone production in hen pituitary cells pretreated with estrogen.
Isolated cells from the pituitary gland of nonlaying hens were preincubated in vitro with or without estradiol-17 beta (E2), and then incubated with or without progesterone (P4), and luteinizing hormone (LH) in the cells and media was measured by a homologous radioimmunoassay. When the cells were preincubated with E2 for 2 h and then incubated with P4 for 4 h, cellular LH was increased, but LH in the medium remained unchanged. The increase in cellular LH in response to P4 was dose-dependent. Protein synthesis inhibitors (cycloheximide, actinomycin-D, and alpha-amanitin) blocked the response of the cells to P4. The results suggest that LH production in estrogen-primed pituitary cells of the hen was stimulated by P4 through a protein-synthesizing pathway.
Kawashima
M
M
Department of Poultry and Animal Sciences, Gifu University, Japan.
Ukai
A
A
Kamiyoshi
M
M
Tanaka
K
K
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000502:physiology*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D007986:Luteinizing Hormone / Q000096:biosynthesis*; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011374:Progesterone / Q000494:pharmacology*
D003470:Culture Media; D004967:Estrogens; D011374:Progesterone; D007986:Luteinizing Hormone