title: Heat shock protein 27 and alpha B-crystallin can form a complex, which dissociates by heat shock.
abstract: We have previously demonstrated that in non-oncogenic adenovirus-transformed baby rat kidney cells a complex of hsp27 and a 22-kDa protein is present, which is lacking in oncogenic cells (Zantema, A., de Jong, E., Lardenoije, R., and van der Eb, A. J. (1989) J. Virol. 63, 3368-3375). Here we show that the 22-kDa protein is identical to alpha B-crystallin. The complex of hsp27 and alpha B-crystallin is also found in some other (non-transformed) cells. However, in most cells tested only hsp27 and no alpha B-crystallin is synthesized. Gel filtration studies show that both proteins are present almost exclusively in a 700-kDa complex. Heat treatment makes the complex fall apart, which is accompanied by a change in the conformation of alpha B-crystallin. Upon recovery, complexes are formed again from both pre-existing and newly synthesized proteins.
authors.affiliation: Laboratory for Molecular Carcinogenesis, Sylvius Laboratory, Leiden, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Zantema
authors.forename: M
authors.initials: M
authors.lastname: Verlaan-De Vries
authors.forename: D
authors.initials: D
authors.lastname: Maasdam
authors.forename: S
authors.initials: S
authors.lastname: Bol
authors.forename: A
authors.initials: A
authors.lastname: van der Eb
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D003459:Crystallins / Q000378:metabolism*; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D008970:Molecular Weight; D011233:Precipitin Tests
chemical_list: D003459:Crystallins; D006360:Heat-Shock Proteins