title: Fluoride binding by matrix proteins in rat mineralizing tissue.
abstract: Chronic fluoride exposure in vivo results in alterations in the formation of mineralizing tissues. One possible mechanism for the formation of fluorosed tooth enamel and bone is a binding of fluoride to matrix proteins, resulting in an alteration in their structure and function. Studies were designed to investigate fluoride binding to matrix proteins in vivo and their possible role in fluorosis. Rats were given either 0 or 100 parts/10(6) fluoride in drinking water for 6 weeks to allow the formation of fluorotic mineralizing tissues. The animals were killed by CO2 inhalation, and the enamel and bone were analysed for fluoride and calcium. Matrix binding by fluoride in enamel was determined after extraction of proteins from undemineralized matrix. In bone, the matrix was demineralized and F, Ca and P were determined in both ashed and unashed samples. The studies showed ionic binding of fluoride to the matrix in both enamel and bone, possibly associated with calcium binding by the matrix. There was no difference in the amount of matrix-bound fluoride in control as compared to fluorosed bone or maturation-stage enamel. This indicates that although matrix proteins can bind fluoride, it is not likely that this mechanism is important in the formation of fluorosed mineralizing tissues.
authors.affiliation: Forsyth Dental Center, Boston, MA 02115.
authors.forename: P K
authors.initials: PK
authors.lastname: DenBesten
authors.forename: L M
authors.initials: LM
authors.lastname: Heffernan
authors.forename: J D
authors.initials: JD
authors.lastname: Featherstone
authors.forename: C P
authors.initials: CP
authors.lastname: Shields
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000737:chemistry / Q000378:metabolism; D001842:Bone and Bones / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000032:analysis; D003743:Dental Enamel / Q000737:chemistry; D003746:Dental Enamel Proteins / Q000032:analysis / Q000378:metabolism*; D005459:Fluorides / Q000032:analysis / Q000378:metabolism*; D010758:Phosphorus / Q000032:analysis; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D051381:Rats
chemical_list: D003746:Dental Enamel Proteins; D011506:Proteins; D010758:Phosphorus; D005459:Fluorides; D002118:Calcium