Identification of two novel human immunodeficiency virus type 1 splice acceptor sites in infected T cell lines.
The regulatory and accessory proteins of human immunodeficiency virus type 1 (HIV-1) are produced from singly or multiply spliced mRNAs. We have used HIV-1-specific oligonucleotide primer pairs in a polymerase chain reaction procedure on RNA from lymphocyte cell lines infected with HIV-1(228,200). The amplified products were analysed by hybridization with splice junction-specific oligonucleotide probes to determine splice donor/splice acceptor combination utilization, subcloned into a plasmid vector and the nucleotide sequences were obtained. Two novel splice acceptor sites have been identified.
Smith
J
J
AIDS Molecular Biology Laboratory, Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Victoria, Australia.
Azad
A
A
Deacon
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D005814:Genes, Viral; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D013601:T-Lymphocytes / Q000382:microbiology*