Cardiac teratogenicity of dichloroethylene in a chick model.
Trichloroethylene (TCE) and dichloroethylene (DCE) are related halogenated aliphatic hydrocarbon industrial solvents that are frequently found as drinking water contaminants. TCE has been implicated as a cardiac teratogen in an epidemiologic study and in a chick model. The purpose of this study was to determine whether DCE was also a cardiac teratogen in the chick embryo. Fertilized White Leghorn chick eggs (n = 418) were inoculated just above the embryo with 30 microL of a test solution on d 3 of incubation. Two control groups were studied: normal saline (n = 96) and the diluent for the DCE, mineral oil (n = 108). DCE was studied at three doses: 5, 20, and 25 microM (n = 76, 62, and 76, respectively). Eggs were coded with a seven-digit number to mask identity. Chicks were terminated on d 18 of incubation, and, after external inspection, hearts and great vessels were dissected macroscopically according to a detailed protocol. Abnormal hearts were reviewed and the diagnosis was agreed upon by three investigators before decoding the seven-digit number and photographing the abnormality. Some embryo death and subsequent tissue autolysis occurred in all groups, but, compared to controls, it was not significantly greater in the treatment group. However, combining all controls and all experimentals, significantly more (p = 0.02) embryonic death occurred in the experimental group. Noncardiac anomalies occurred in 17 embryos and were highest in the saline (four), 5 microM (four), and 20 microM (seven) DCE groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Goldberg
S J
SJ
Department of Pediatrics, University of Arizona, Tucson 85724.
Dawson
B V
BV
Johnson
P D
PD
Hoyme
H E
HE
Ulreich
J B
JB
D000818:Animals; D002642:Chick Embryo; D004000:Dichloroethylenes / Q000633:toxicity*; D006330:Heart Defects, Congenital / Q000139:chemically induced* / Q000473:pathology; D013723:Teratogens / Q000633:toxicity; D013997:Time Factors; D014241:Trichloroethylene / Q000633:toxicity
D004000:Dichloroethylenes; D013723:Teratogens; C029297:vinylidene chloride; D014241:Trichloroethylene