title: Rise and fall of crystallin gene messenger levels during fibroblast growth factor induced terminal differentiation of lens cells.
abstract: Explanted rat lens epithelial cells differentiate synchronously in vitro to lens fiber cells in the presence of basic fibroblast growth factor (bFGF). We have monitored the expression of the three rat crystallin gene families, the alpha-, beta-, and gamma-crystallin genes, during this process. The expression of these gene families is sequentially activated, first the alpha-crystallin genes at Day 1, then the beta-crystallin genes at Day 3, and finally the gamma-crystallin genes at Day 8. The steady state levels of alpha- and beta-crystallin mRNA are not affected by incubation with actinomycin D, suggesting that these mRNAs are stable. Nevertheless, all crystallin mRNAs disappear from the differentiated explants between Days 10 and 11, a process signaled by bFGF. At this time a novel abundant mRNA appears. Cloning and sequencing showed that this mRNA encoded aldose reductase. Our results suggest a novel model for the regulation of crystallin synthesis during lens cell differentiation: a gene pulse delivers a certain amount of stable mRNA, this mRNA is removed at a later stage of differentiation by a stage-specific breakdown mechanism. Each of these regulatory steps requires a signal from bFGF.
authors.affiliation: Department of Molecular Biology, University of Nijmegen, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Peek
authors.forename: J W
authors.initials: JW
authors.lastname: McAvoy
authors.forename: N H
authors.initials: NH
authors.lastname: Lubsen
authors.forename: J G
authors.initials: JG
authors.lastname: Schoenmakers
mesh_terms: D000367:Age Factors; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003459:Crystallins / Q000096:biosynthesis / Q000235:genetics*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D051381:Rats
chemical_list: D003459:Crystallins; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2