Ellipsometric immunosensors for the determination of gamma-interferon and human serum albumin.
Ellipsometric immunosensors for gamma-interferon (gamma-INF) and human serum albumin (HSA) have been developed using gamma-INF and HSA covalently immobilized onto silicon plates. Detection is achieved by measuring the adsorption decrease (gamma, mg m-2) of monoclonal immunoglobulin G against gamma-INF or immunoglobulin fraction of rabbit blood serum against HSA when the concentration of antigen in mixtures of antibody-antigen is increased. The time of analysis is 50 min, the detection limit of gamma-INF is 15 nM (sensitivity -0.01 mg m-2 nM-1) and of HSA 2.5 nM (-0.06 mg m-2 nM-1).
Ruzgas
T A
TA
Department of Enzyme Chemistry, Institute of Biochemistry, Lithuanian Academy of Sciences.
Razumas
V J
VJ
Kulys
J J
JJ
D000911:Antibodies, Monoclonal; D015374:Biosensing Techniques; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007371:Interferon-gamma / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis*; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D012709:Serum Albumin; D007371:Interferon-gamma