title: High-performance liquid chromatographic determination of rifapentine in serum using column switching.
abstract: A high-performance liquid chromatographic method with column switching has been developed for the determination of rifapentine in serum. The serum samples were injected onto a precolumn packed with Corasil RP C18 (37-50 microns) after simple dilution with an internal standard in a 1% ascorbic acid solution. Polar serum components were washed out using 0.05 M phosphate buffer. After valve switching, the concentrated drugs were eluted in the back-flush mode and separated by a mu Bondapak C18 column with acetonitrile-tetrahydrofuran-0.05 M phosphate buffer (pH 7.0) (42:5:53, v/v/v) as the mobile phase. The method showed excellent precision with good sensitivity and speed, and a detection limit of 0.1 microgram/ml. The total analysis time was less than 25 min and the mean coefficients of variation for intra- and inter-assay were less than 4.8%. The method has been successfully applied to serum samples from dogs after the oral administration of rifapentine.
authors.affiliation: Department of Pharmacology, Korea Research Institute of Chemical Technology, Daejeon, South Korea.
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.forename: H C
authors.initials: HC
authors.lastname: Shin
authors.forename: S S
authors.initials: SS
authors.lastname: Han
authors.forename: J K
authors.initials: JK
authors.lastname: Roh
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D007917:Leprostatic Agents / Q000097:blood*; D015203:Reproducibility of Results; D012293:Rifampin / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D007917:Leprostatic Agents; D012293:Rifampin; C018421:rifapentine