title: Amino acid sequence of the N-terminal domain of yam (Dioscorea japonica) aerial tuber acidic chitinase. Evidence for the presence of a wheat germ agglutinin domain in matured acidic chitinase from unstressed tuber.
abstract: The amino acid sequence of the N-terminal domain of acidic chitinase from unstressed aerial tuber was determined and proved the presence of an N-terminal domain in acidic chitinase. The amino acid sequence was determined on a pyroglutamylaminopeptidase-treated N-terminal fragment of V8 protease and on chymotryptic peptides of this fragment. The sequence determined revealed 8 residues deletion and 2 residues insertion as compared with the N-terminal domain of tobacco basic chitinase. The N-terminal domain determined showed a homology of 40% and 52% with the N-terminal domain of tobacco basic chitinase and wheat germ agglutinin, respectively.
authors.affiliation: Department of Biochemistry, School of Agriculture, Kyushu Tokai University, Kumamoto, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Araki
authors.forename: J
authors.initials: J
authors.lastname: Funatsu
authors.forename: M
authors.initials: M
authors.lastname: Kuramoto
authors.forename: T
authors.initials: T
authors.lastname: Torikata
mesh_terms: D000595:Amino Acid Sequence; D002688:Chitinases / Q000737:chemistry* / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D037121:Plant Lectins; D010944:Plants / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases; D014909:Wheat Germ Agglutinins / Q000737:chemistry*
chemical_list: D010446:Peptide Fragments; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins; D002688:Chitinases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase