title: Fluorometric determination of the DNA content of cells cultured in tissue culture plates.
abstract: Determining the DNA content of cultured cells with the fluorochrome diaminobenzoic acid (DABA) has been shown to be a sensitive and selective method. A procedure is described which applies this method to cells cultured in 96-well and 24-well plates. After removal of the medium, the cells are disrupted by sonication, dried and reacted with DABA. For the final read-out the DABA-DNA solution is transferred to standard cuvettes and the fluorescence intensity determined with a conventional fluorometer. The method is highly reproducible and suitable for the cell concentrations usually employed in microculture systems. It is an easy non-radioactive method for determining the numbers of both adherent and non-adherent cells, especially when comparing different stages of activation or differentiation.
authors.affiliation: Institute of Molecular Pharmacology, Medical School Hannover, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Goppelt-Struebe
authors.forename: M
authors.initials: M
authors.lastname: Golombek
mesh_terms: D062365:Aminobenzoates; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes; D006801:Humans; D013050:Spectrometry, Fluorescence; D013755:Tetradecanoylphorbol Acetate; D014407:Tumor Cells, Cultured
chemical_list: D062365:Aminobenzoates; D005456:Fluorescent Dyes; D004247:DNA; D013755:Tetradecanoylphorbol Acetate