title: In vivo function of regulatory DNA sequence elements of a major histocompatibility complex class I gene.
abstract: Major histocompatibility complex class I genes are expressed in nearly all somatic tissues, although their level of expression varies. By analysis of a set of promoter deletion mutants introduced into transgenic mice, a complex regulatory element, consisting of overlapping enhancer and silencer activities, is demonstrated to function as a tissue-specific regulator of class I expression. The enhancer activity predominates in lymphoid tissues but not in nonlymphoid tissues. In contrast to the tissue-specific functions of the complex regulatory element, a second novel silencer element is shown to function in both lymphoid and nonlymphoid tissues. The complement of DNA-binding factors in different cell lines is shown to correlate with the levels of class I expression.
authors.affiliation: Experimental Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.
authors.forename: J E
authors.initials: JE
authors.lastname: Maguire
authors.forename: W I
authors.initials: WI
authors.lastname: Frels
authors.forename: J C
authors.initials: JC
authors.lastname: Richardson
authors.forename: J D
authors.initials: JD
authors.lastname: Weissman
authors.forename: D S
authors.initials: DS
authors.lastname: Singer
mesh_terms: D000818:Animals; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D005805:Genes, MHC Class I / Q000235:genetics*; D008221:Lymphoid Tissue / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C075396:PD1 protein, pig; D011994:Recombinant Proteins