title: Histopathological and biochemical effects of gossypol acetate on pituitary-gonadal axis of male albino rats.
abstract: Histopathological and biochemical effects of gossypol acetate (GA) on pituitary-gonadal axis were investigated. 10 and 25 mg GA/kg were administered orally to sexually mature adult male Wistar rats for 4 and 5 weeks, respectively. STH and LTH/PRL cells showed no significant changes as compared to those of controls while TSH cells showed hypertrophy, hyperplasia and degranulation in both experimental groups. Pituitary FSH, LH/ICSH cells showed progressive regression. Gonosomatic indices of sex accessory glands at 25 mg showed significant reduction in the experimental animals as compared to those of controls. The diameter of seminiferous tubules reduced and azoospermia developed. Sertoli and Leydig cells also regressed. At 10 and 25 mg GA treatment, spermatogenesis ceased at secondary spermatocytes and spermatogonia stages, respectively. Epididymis and prostate regressed. Seminal vesicle showed no significant histological variations as compared to that of control except reduction in secretion. Biochemical observations revealed increased levels of acid phosphatase, fructose and citric acid and significant reduction in glycerylphosphoryl choline in reproductive glands of both experimental groups as compared to those of controls. Possible mechanism of antifertility action of GA is discussed.
authors.affiliation: Department of Zoology, Nagpur University Campus, India.
authors.forename: P
authors.initials: P
authors.lastname: Udoh
authors.forename: D R
authors.initials: DR
authors.lastname: Patil
authors.forename: M K
authors.initials: MK
authors.lastname: Deshpande
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002951:Citrates / Q000032:analysis; D019343:Citric Acid; D004822:Epididymis / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D005640:Follicle Stimulating Hormone / Q000032:analysis; D005632:Fructose / Q000032:analysis; D005997:Glycerylphosphorylcholine / Q000032:analysis; D006072:Gossypol / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000494:pharmacology; D007985:Leydig Cells / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D007986:Luteinizing Hormone / Q000032:analysis; D008297:Male; D009845:Oligospermia / Q000139:chemically induced; D010902:Pituitary Gland / Q000737:chemistry / Q000187:drug effects / Q000473:pathology*; D011388:Prolactin / Q000032:analysis; D011467:Prostate / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D051381:Rats; D012708:Sertoli Cells / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D013089:Spermatocidal Agents / Q000009:adverse effects / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000737:chemistry / Q000187:drug effects / Q000473:pathology*; D013997:Time Factors
chemical_list: D002951:Citrates; D013089:Spermatocidal Agents; D019343:Citric Acid; D005632:Fructose; D005997:Glycerylphosphorylcholine; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D000135:Acid Phosphatase; D006072:Gossypol; C028178:gossypol acetic acid