alpha-Amylases and approaches leading to their enhanced stability.
The recent state of the knowledge of properties and structure of alpha-amylases is reviewed with the aim of elucidation the basis for their stabilization. Three principal ways for obtaining stable alpha-amylases (isolation of enzymes from extremophiles, production of extremophilic enzymes in mesophiles, and modification of mesophilic enzymes) are discussed separately. Detailed experimental examples are given for modification approaches.
Janecek
S
S
Department of Biochemical Technology, Slovak Technical University, Bratislava, Czech and Slovak Federal Republic.
Baláz
S
S
D004795:Enzyme Stability; D000516:alpha-Amylases / Q000378:metabolism*
D000516:alpha-Amylases