[Estimation of glucocorticoid membrane binding sites in synaptic plasma membrane isolated from porcine brain].
Using radioligand binding assay, we measured the Bmax and Kd of glucocorticoid membrane binding sites (GCMBS) in the synaptic plasma membranes isolated from different regions of porcine brain. The Kds of GCMBS in cerebrum, cerebellum, midbrain, thalamus, nuclei caudatus and hypothalamus are all around 200 nmol/L. The Bmax of GCMBS in the mentioned brain regions ranges from 218.98 fmol/mg to 486.95 fmol/mg protein.
Fu
H
H
Department of Physiology, Second Military Medical University, Shanghai.
Guo
Z
Z
Chen
Y Z
YZ
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D011869:Radioligand Assay; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D013552:Swine; D013570:Synaptic Membranes / Q000378:metabolism*
D011965:Receptors, Glucocorticoid