Localization of MPP+ binding sites in the brain of various mammalian species.
The distribution and density of 3H-MPP+ binding sites were studied by in vitro quantitative autoradiography in the brain of the mouse, rat and monkey. The highest levels of 3H-MPP+ specific binding were observed in rat brain. The substantia nigra in rat and monkey, and the anterior caudate-putamen formation in mouse and monkey showed the lowest density of autoradiographic grains. The presence of a relatively high density of MPP+ sites in the hippocampus of all species studied could be of interest to explain some effects of MPTP administration on convulsions caused by chemoconvulsants. The finding of a 60-70% reduction of 3H-MPP+ binding sites in the rat caudate-putamen, on the side of quinolinic acid infusion and no changes after 6-hydroxydopamine lesion of dopaminergic nigrostriatal neurons suggests the presence of these sites mainly on striatal cells. The results suggest that the distribution of MPP+ binding sites in brain would not seem to be related to MPTP toxicity.
Del Zompo
M
M
Department of Neurosciences B. B. Brodie, University of Cagliari, Italy.
Piccardi
M P
MP
Ruiu
S
S
Albanese
A
A
Morelli
M
M
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000493:pharmacokinetics; D000818:Animals; D001665:Binding Sites; D001923:Brain Chemistry; D002144:Callithrix / Q000235:genetics*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007091:Image Processing, Computer-Assisted; D008297:Male; D051379:Mice / Q000378:metabolism*; D008995:Monoamine Oxidase / Q000378:metabolism; D016627:Oxidopamine / Q000633:toxicity; D011726:Pyridinium Compounds / Q000378:metabolism* / Q000493:pharmacokinetics; D051381:Rats / Q000378:metabolism*; D011919:Rats, Inbred Strains / Q000378:metabolism; D013045:Species Specificity
D011726:Pyridinium Compounds; D016627:Oxidopamine; C044755:1-methyl-4-phenyl-2,3-dihydropyridinium; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D008995:Monoamine Oxidase