Restoration of mitochondrial energy-linked reactions following dexamethasone treatment of rats infected with the liver fluke Fasciola hepatica.
Mitochondria isolated from male Wistar rats experimentally infected with the common liver fluke, Fasciola hepatica, exhibit loss of respiratory control from 2 weeks post-infection (Rule, et al. (1989) Biochem. J. 260, 517-523). We now report that subcutaneous injections of the anti-inflammatory drug, dexamethasone, during the final week of infection prevented the mitochondrial uncoupling and restored respiratory control almost to the levels of uninfected controls. Further investigations have shown that mitochondria from infected rat livers are unable to synthesize ATP and that abnormal respiration is also evident in hepatocytes isolated from infected rats. These abnormalities were absent when infected rats were treated with dexamethasone. In addition, liver mitochondrial function in infected, congenitally athymic, nude rats (CBH/R nu/nu) was not significantly different from that in uninfected nude or Wistar controls. These results provide evidence that the mitochondrial dysfunction in fascioliasis is host-mediated and that T lymphocytes in particular may be involved.
Hanisch
M J
MJ
Division of Biochemistry and Molecular Biology, School of Life Sciences, Australian National University, Canberra.
Topfer
F
F
Lenton
L M
LM
Behm
C A
CA
Bygrave
F L
FL
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D005210:Fasciola hepatica; D005211:Fascioliasis / Q000378:metabolism*; D008099:Liver / Q000469:parasitology; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011923:Rats, Nude
D003907:Dexamethasone; D000255:Adenosine Triphosphate