Anomalous binding of [3H]N-methyl-quinuclidinyl benzilate methyl chloride to human lymphocyte muscarinic receptors.
Using the muscarinic cholinergic ligand [3H]N-methyl quinuclidinyl benzilate methyl chloride ([3H]NM-QNB), we demonstrated that intact, viable human lymphocytes possess specific muscarinic binding sites. Equilibrium binding studies show that muscarinic acetylcholine receptor are divided into two subtype; high affinity (Ms) and low affinity types (Mw) for the ligand.
Usami
K
K
Department of Neuropsychiatry, Yamaguchi University School of Medicine, Japan.
Kaku
Y
Y
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008875:Middle Aged; D010276:Parasympatholytics / Q000378:metabolism*; D011813:Quinuclidinyl Benzilate / Q000031:analogs & derivatives* / Q000378:metabolism; D011869:Radioligand Assay; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012016:Reference Values
D010276:Parasympatholytics; D011976:Receptors, Muscarinic; C028484:quinuclidinyl benzilate receptor; D011813:Quinuclidinyl Benzilate; C054940:clidinium