Activity of liposomal interleukin-2 in vitro.
Preclinical in vitro assessment of highly purified natural human interleukin-2 (IL-2) packed in egg lecithin liposomes was performed in short- and long-term T-cell cloning and propagation systems, and in experiments testing induction of lymphokine-activated killer (LAK) cells. Liposomal IL-2 (lip-IL-2) was essentially as active as free natural or recombinant IL-2 for cloning and culture of both helper and cytotoxic alloreactive T cells. However, lip-IL-2 was found to be markedly inferior to free natural or recombinant IL-2 for the induction of LAK cells from normal donors. Nevertheless, lip-IL-2 was able to maintain LAK cytotoxicity of populations preactivated with free IL-2. These results suggest that lip-IL-2 can interact with activated T cells and LAK cells in the same way as free IL-2, but that it is much less efficient at activating LAK-cell precursors.
Adibzadeh
M
M
Second Department of Internal Medicine, University Medical Clinic, Tübingen, Germany.
Weder
H G
HG
Rehbein
A
A
Schwuléra
U
U
Obermeier
J
J
Pawelec
G
G
D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000008:administration & dosage*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D008081:Liposomes; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology
D007376:Interleukin-2; D008081:Liposomes