Polymorphism at codon 72 of the p53 gene in human acute myelogenous leukemia.
A common polymorphism at codon 72 of the p53 gene in patients with acute myelogenous leukemia (AML) was analyzed by single-strand conformation polymorphism assay and sodium dodecyl sulfate polyacrylamide-gel electrophoresis of immunoprecipitated 35S-labeled P53 protein. No association between this polymorphism and a marked predisposition to AML was found. The half-lives of these two polymorphic forms of P53 were equivalent in normal phytohemagglutinin-stimulated lymphocytes, while the P53 Pro72 isoform was found to be twice as stable as the Arg72 isoform in Daudi cells.
Zhang
W
W
Department of Hematology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Hu
G
G
Deisseroth
A
A
D001483:Base Sequence; D003062:Codon / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics; D004586:Electrophoresis; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
D003062:Codon; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides