Plant protoplasts as genetic tool: selectable markers for developmental studies.
Protoplasts have usually been presented as a methodological tool. Even as such, they make possible an impressive array of applications in plant biology. Here we report on the use of protoplast-derived selectable markers in the study of several disturbed genetic systems with obvious effects on plant development: (1) auxotrophic mutants and the control of amino acid biosynthesis and transport in vegetative and reproductive tissues; (2) introgression of alien genetic information across phylogenetic boundaries by protoplast fusion, a consequence of controlled dedifferentiation-redifferentiation processes and attenuated incompatibility reactions in cultured cells; (3) expression (in)stability of foreign genes in transgenic plants during successive meiotic generations and in crosses between independent transformants.
Negrutiu
I
I
Laboratory of Plant Genetics, Vrije Universiteit Brussel, Belgium.
Hinnisdaels
S
S
Cammaerts
D
D
Cherdshewasart
W
W
Gharti-Chhetri
G
G
Jacobs
M
M
D002478:Cells, Cultured; D005821:Genetic Techniques; D009154:Mutation / Q000235:genetics; D059828:Plant Cells; D010944:Plants / Q000235:genetics*; D011523:Protoplasts / Q000166:cytology*