title: Glutamate decarboxylase in developing rat neocortex: does it correlate with the differentiation of GABAergic neurons and synapses?
abstract: Postnatal development of glutamate decarboxylase was studied in the rat cerebral cortex. Two methods were used: estimation of the enzymatic activity of glutamate decarboxylase in homogenates of developing cortical tissue and visualization of structures containing glutamate decarboxylase-like immunoreactivity. Glutamate decarboxylase-like immunoreactivity appeared first in perikarya and dendrites and only later in axons and axon varicosities. The most rapid increase in the glutamate decarboxylase activity took place during the second postnatal week and this coincided with a rapid increase in the density of axon varicosities containing glutamate decarboxylase-like immunoreactivity but preceded the most rapid phase in the formation of GABAergic synapses by several days. However, there was a change in the characteristics of glutamate decarboxylase which correlated with GABA synaptogenesis: two fractions of glutamate decarboxylase with different sensitivities to the activating effects of Triton X-100 could be distinguished as from about the time when most of the GABAergic synapses are formed.
authors.affiliation: Department of Anatomy, University of Sydney, Australia, N.S.W.
authors.forename: V J
authors.initials: VJ
authors.lastname: Balcar
authors.forename: T
authors.initials: T
authors.lastname: Zetzsche
authors.forename: J R
authors.initials: JR
authors.lastname: Wolff
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002540:Cerebral Cortex / Q000201:enzymology* / Q000254:growth & development; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase