Changes in the migratory ability of human lung and skin fibroblasts during in vitro aging and in vivo cellular senescence.
The migration of human lung and skin fibroblasts was determined during in vitro aging and in vivo cellular senescence by measuring their migration from the edge of a denuded area of a monolayer. The migration of human fetal lung fibroblasts (TIG-1 and TIG-3) decreased only very slightly with increasing passage, whereas the migration of human fetal skin fibroblasts (TIG-3S) declined gradually: the difference in cell migratory ability between early and late passages was significant (P less than 0.05). The migratory patterns of skin fibroblasts from adult and elderly donors were also similar to that of fetal skin fibroblasts. Next, the migratory abilities of fibroblast lines from adult and elderly donor groups were compared, using relatively early passaged cells. The migratory ability of the elderly-donor skin fibroblast lines was significantly lower (P less than 0.05) than that of the adult-donor skin fibroblast lines. Addition of suramin and monensin suppressed the migration of fibroblasts from fetal, adult and elderly donors, which implies that fibroblast migration is regulated by growth factors and matrix substances. The relationships between the age-dependent decline of migratory ability, growth factors and the extracellular matrix are discussed.
Kondo
H
H
Department of Experimental Biology, Tokyo Metropolitan Institute of Gerontology, Japan.
Yonezawa
Y
Y
D000328:Adult; D000368:Aged; D000375:Aging; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D016922:Cellular Senescence; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000166:cytology; D008985:Monensin / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D013498:Suramin / Q000494:pharmacology
D013498:Suramin; D008985:Monensin