Appearance of phloridzin-sensitive glucose transport is not controlled at mRNA level in rabbit jejunal enterocytes.
Glucose uptake by rabbit intestine has been shown to be confined to the upper two-thirds of jejunal villi. Inhibition by phloridzin shows most of this transport to take place through the Na(+)-glucose linked transporter (SGLT1). Parallel measurements on the other hand show SGLT1 mRNA expression to increase rapidly as enterocytes reach the crypt-villus junction. Levels of SGLT1 mRNA then remain elevated in all villus enterocytes. These results provide direct evidence that SGLT1-mediated glucose transport is subject to post-transcriptional control.
Smith
M W
MW
Department of Cell Biology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge.
Turvey
A
A
Freeman
T C
TC
D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D005260:Female; D015870:Gene Expression; D005947:Glucose / Q000493:pharmacokinetics*; D007583:Jejunum / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D008871:Microvilli / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000502:physiology; D010695:Phlorhizin / Q000494:pharmacology*; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology*; D011817:Rabbits; D014158:Transcription, Genetic
D009004:Monosaccharide Transport Proteins; D010695:Phlorhizin; D005947:Glucose