A method for determination of transketolase activity based on the use of a pH indicator.
A new method for assaying transketolase activity is proposed. The method consists in recording the pH changes in the course of the enzymatic reaction and is based on the use of the pH-indicator p-nitrophenol. When p-nitrophenol is added to a reaction mixture containing hydroxypyruvate and glycolaldehyde as substrates the absorbance increases. The rate of the change of absorbance is proportional to the enzyme concentration.
Hübner
G
G
Institute of Biochemistry, Martin-Luther-University, Halle-Wittenberg, Germany.
Golbik
R
R
Meshalkina
L E
LE
D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D008722:Methods; D009596:Nitrophenols; D013057:Spectrum Analysis; D014174:Transketolase / Q000378:metabolism*
D007202:Indicators and Reagents; D009596:Nitrophenols; D014174:Transketolase; C024836:4-nitrophenol