title: [Serological screening for Trypanosoma cruzi among blood donors in central Brazil].
abstract: The present study compares the results of serological screening for Trypanosoma cruzi infection done at blood banks with results obtained in Chagas' disease studies undertaken by the Reference Laboratory of the Federal University of Goiás (UFG) and evaluates the use of the enzyme-linked immunosorbent assay (ELISA) for this purpose. The study was conducted with data from six of the eight blood banks in the city of Goiânia in central Brazil, an urban area in which this infection is highly endemic. The population studied consisted of 1,513 volunteers who had donated blood for the first time between October 1988 and April 1989. The sample represented 50% of all first-time blood donors during the period. Of these donors, 94% were residents of urban areas, and of these, approximately 26% had migrated from the countryside. Nearly 90% of the blood donations in the city are received at these banks, which normally use the indirect hemagglutination and complement-fixation tests. The samples selected for the study of T. cruzi antibody in first-time blood donors were assayed at the Reference Laboratory of the Federal University of Goiás using the indirect hemagglutination (IH), indirect immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) tests, independently of the serological classification performed by the blood banks. Comparison of the results provided by the latter with the positivity pattern established in the study (IH and IF yielded simultaneous positive results in the Reference Laboratory) revealed a relative sensitivity of 77%, with extremes ranging between 50% and 100%, depending on the blood bank studied.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Brasil.
authors.forename: A L
authors.initials: AL
authors.lastname: de Andrade
authors.forename: C M
authors.initials: CM
authors.lastname: Martelli
authors.forename: A O
authors.initials: AO
authors.lastname: Luquetti
authors.forename: O S
authors.initials: OS
authors.lastname: de Oliveira
authors.forename: S
authors.initials: S
authors.lastname: Almeida e Silva
authors.forename: F
authors.initials: F
authors.lastname: Zicker
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001771:Blood Banks / Q000592:standards; D001782:Blood Donors; D001938:Brazil / Q000453:epidemiology; D014355:Chagas Disease / Q000097:blood / Q000453:epidemiology / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008403:Mass Screening; D011237:Predictive Value of Tests; D014349:Trypanosoma cruzi / Q000276:immunology*; D014505:Urban Population
chemical_list: D000913:Antibodies, Protozoan
vernacular_title: Triagem sorológica para o Trypanosoma cruzi entre doadores de sangue do Brasil central.