Corticosterone 6 beta-hydroxylation correlates with blood pressure in spontaneously hypertensive rats.
Evidence for increased glucocorticoid 6 beta-hydroxylation (enhanced family 3A cytochrome P-450 activity) is found in certain reversible forms of human hypertension. This association was investigated in the spontaneously hypertensive rat (SHR). The proportion of injected [3H]corticosterone excreted in urine as 6 beta-[3H]OH-corticosterone was four- to fivefold higher in SHR than in control Wistar-Kyoto rats, before and after development of overt hypertension. Both hypertension and 6 beta-hydroxylation were inhibited by troleandomycin (a selective inhibitor of family 3A cytochromes P-450), consistent with a role for increased steroid 6 beta-hydroxylation in the genesis of hypertension in the SHR.
Watlington
C O
CO
Division of Endocrinology and Metabolism, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0145.
Kramer
L B
LB
Schuetz
E G
EG
Zilai
J
J
Grogan
W M
WM
Guzelian
P
P
Gizek
F
F
Schoolwerth
A C
AC
D000818:Animals; D001794:Blood Pressure; D003345:Corticosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000652:urine; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY / Q000378:metabolism; D013599:Systole; D014217:Troleandomycin / Q000494:pharmacology
C044550:6-hydroxycorticosterone; D014217:Troleandomycin; D003345:Corticosterone