Affinity-mediated modification of electrical charge on a cell surface: a new approach to the affinity partitioning of biological particles.
Polylysine has been covalently bound to human transferrin in a 1:1 molar ratio over a disulfide bond that can be easily split by reducing agents such as dithiothreitol. The association constant for the binding of the transferrin-polylysine derivative to transferrin receptors present on rat erythroblasts and the number of binding sites were identical to the corresponding values found for native transferrin. The incubation of the cells with transferrin-polylysine affected the partitioning of erythroblasts in a charge-sensitive aqueous two-phase system containing Dextran and polyethylene glycol. The polylysine part introduced a nonspecific influence on the partitioning that could be eliminated by preincubation of the cells with an excess of sialic acid. The partition ratio, G, of the erythroblasts changed with a factor of 1.9 for each set of 100,000 polylysine chains attached per cell.
Mendieta
J
J
Department of Biochemistry, University of Lund, Sweden.
Johansson
G
G
D000818:Animals; D001667:Binding, Competitive; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D002850:Chromatography, Gel; D004900:Erythroblasts / Q000166:cytology*; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008297:Male; D011107:Polylysine / Q000031:analogs & derivatives; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D011990:Receptors, Transferrin / Q000378:metabolism; D014168:Transferrin / Q000031:analogs & derivatives
D007202:Indicators and Reagents; D011990:Receptors, Transferrin; D014168:Transferrin; C072481:transferrin-polylysine conjugate; D011107:Polylysine