Embryonic cytoplasmic extracts rescue murine androgenones to the blastocyst stage.
Androgenones (paternally derived genome) show a significant inability to form a blastocoele cavity. Eighty percent of these embryos die or arrest at earlier stages. Factor(s) from both normal and parthenogenetic late preimplantation embryos injected into each blastomere of androgenetic 4-cell stage can rescue more than twice as many to the blastocyst stage (47.2% versus 19.2% for non-injected androgenones). This factor(s) becomes available beginning at the 4-cell stage and is titratable. Injected total cytoplasmic mRNA will also cause a rescue response. Isolating this specific factor message(s) will permit the eventual cloning of possibly the earliest parentally imprinted gene(s) expressed during development.
Hagemann
L J
LJ
Department of Scientific and Industrial Research, Palmerston North, New Zealand.
First
N L
NL
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D003593:Cytoplasm / Q000502:physiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010312:Parthenogenesis / Q000502:physiology