Effect of selenium-deficient diet in experimental glomerular disease.
We examined the effect of a selenium-deficient diet on two experimental models of glomerular disease, the puromycin aminonucleoside (PAN)-induced nephrotic syndrome, a model of minimal change disease, and passive Heymann nephritis, a complement-dependent and neutrophil-independent model that resembles membranous nephropathy. The specific activity of selenium-dependent glutathione peroxidase was markedly reduced in the liver, the kidney cortex, and in glomeruli in weanling male Sprague-Dawley rats placed on a selenium-deficient diet for 6 wk compared with rats fed a selenium-replete diet, with no significant differences in the specific activities of superoxide dismutase or catalase. PAN-injected selenium-deficient rats had a marked and significantly greater proteinuria throughout the course of the experiment compared with PAN-injected selenium-replete rats with no significant histological differences. In the passive Heymann nephritis model induced by injecting anti-Fx1A immunoglobulin G, rats fed a selenium-deficient diet had significantly higher urinary protein (day 5: 91 +/- 16 mg/24 h, n = 10) compared with rats fed a selenium-replete diet (52 +/- 5 mg/24 h, n = 11) with no differences in the amount of antibody deposited in the kidney. The most likely explanation for the effect of a selenium-deficient diet is that selenium deficiency resulted in a marked reduction of glutathione peroxidase, thus indicating an important role of glutathione peroxidase in these models of glomerular injury.
Baliga
R
R
Department of Pediatrics, Louisiana State University School of Medicine, New Orleans 70112.
Baliga
M
M
Shah
S V
SV
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D004032:Diet; D005979:Glutathione Peroxidase / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D007672:Kidney Cortex / Q000201:enzymology; D007674:Kidney Diseases / Q000201:enzymology; D007678:Kidney Glomerulus / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D009404:Nephrotic Syndrome / Q000139:chemically induced / Q000201:enzymology; D011507:Proteinuria / Q000201:enzymology / Q000276:immunology; D011692:Puromycin Aminonucleoside; D051381:Rats; D012643:Selenium / Q000008:administration & dosage* / Q000172:deficiency
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D011692:Puromycin Aminonucleoside; D005979:Glutathione Peroxidase; D012643:Selenium