Induction of thromboxane synthase and prostaglandin endoperoxide synthase mRNAs in human erythroleukemia cells by phorbol ester.
The effects of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on the mRNA levels of two enzymes, thromboxane synthase (TXS) and prostaglandin endoperoxide synthase (PES), responsible for the synthesis of thromboxane A2 from arachidonic acid, were studied in human erythroleukemia (HEL) cells by RNA blot analysis. TPA induced both TXS and PES mRNAs in HEL cells in a dose-dependent manner at 36 h. The half-maximal and maximal effects for the induction of both mRNAs were at approximately 3 x 10(-9) M and at 10(-8) M, respectively. TXS and PES mRNA levels increased in a time-dependent fashion by TPA, and reached to 7- and 3.5-fold of the control, respectively after 48 h of TPA treatment. These results suggest that expression of TXS and PES genes in HEL cells were simultaneously stimulated by TPA.
Ihara
H
H
Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan.
Yokoyama
C
C
Miyata
A
A
Kosaka
T
T
Nüsing
R
R
Ullrich
V
V
Tanabe
T
T
D016861:Cyclooxygenase Inhibitors / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D012333:RNA, Messenger / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013930:Thromboxane-A Synthase / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D016861:Cyclooxygenase Inhibitors; D012333:RNA, Messenger; D013930:Thromboxane-A Synthase; D013755:Tetradecanoylphorbol Acetate