title: Plausible structure of the iron-molybdenum cofactor of nitrogenase.
abstract: A plausible structure of the iron-molybdenum cofactor of nitrogenase [reduced ferredoxin:dinitrogen oxidoreductase (ATP-hydrolyzing), EC 1.18.6.1] is presented based on altered substrate reduction properties of dinitrogenase containing homocitrate analogs within the cofactor. Alterations on each carbon of the four-carbon homocitrate backbone were correlated with altered substrate reduction properties of dinitrogenase containing these analogs. Altered substrate reduction properties are the basis for a model in which homocitrate is oriented about two cubane metal clusters.
authors.affiliation: Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
authors.forename: M S
authors.initials: MS
authors.lastname: Madden
authors.forename: A M
authors.initials: AM
authors.lastname: Krezel
authors.forename: R M
authors.initials: RM
authors.lastname: Allen
authors.forename: P W
authors.initials: PW
authors.lastname: Ludden
authors.forename: V K
authors.initials: VK
authors.lastname: Shah
mesh_terms: D005561:Formates / Q000737:chemistry; D066298:In Vitro Techniques; D015394:Molecular Structure; D008983:Molybdoferredoxin / Q000648:ultrastructure*; D009591:Nitrogenase / Q000378:metabolism / Q000648:ultrastructure*; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014233:Tricarboxylic Acids / Q000737:chemistry
chemical_list: D005561:Formates; D008983:Molybdoferredoxin; D014233:Tricarboxylic Acids; C028143:homocitric acid; D009591:Nitrogenase