Ribosome function determined by fluorescence.
Five different fluorescence phenomena are considered in relation to their use to study the structure and function of ribosomes. These are: quantum yield or emission intensity; emission wavelength maximum; fluorescence anisotropy; collisional quenching; and nonradiative energy transfer. Results from a number of studies in which these techniques were used are described and summarized in relation to the movement and conformation of tRNA, the nascent peptide, and mRNA in a ribosome during the reaction steps of peptide elongation.
Hardesty
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Department of Chemistry and Biochemistry, University of Texas, Austin 78712.
Odom
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Picking
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D001483:Base Sequence; D005453:Fluorescence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000737:chemistry / Q000502:physiology; D012343:RNA, Transfer / Q000737:chemistry / Q000502:physiology; D012270:Ribosomes / Q000737:chemistry* / Q000502:physiology*; D013050:Spectrometry, Fluorescence
D012333:RNA, Messenger; D012343:RNA, Transfer