title: Binding of Saccharomyces cerevisiae extracellular proteins to glucane.
abstract: Interactions of Saccharomyces cerevisiae cell wall proteins with purified yeast glucane were studied. Using the beta-glucanase (BGL2 gene product) as the model cell wall protein, strong binding to glucane was demonstrated at pH lower than 7, while at pH higher than 8 the reaction did not occur. NaCl (2 M) did not influence the binding, while urea in concentrations higher than 4 M affected the interactions. It was also found that most other cell wall proteins, as well as intracellular proteins, reacted with glucane in the same way, showing that the interactions of proteins with glucane are rather nonspecific. Soluble periplasmic proteins invertase and acid phosphatase failed to react with glucane under the same conditions, indicating that these proteins have certain structural features preventing their interactions with glucane.
authors.affiliation: Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia.
authors.forename: V
authors.initials: V
authors.lastname: Mrsa
authors.forename: T
authors.initials: T
authors.lastname: Ugarković
authors.forename: S
authors.initials: S
authors.lastname: Barbarić
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry*; D005656:Fungal Proteins / Q000378:metabolism*; D043326:Glucan 1,3-beta-Glucosidase; D005936:Glucans / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D011134:Polysaccharides / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D012965:Sodium Chloride / Q000494:pharmacology; D012995:Solubility; D014508:Urea / Q000494:pharmacology; D043324:beta-Fructofuranosidase; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D011134:Polysaccharides; D012965:Sodium Chloride; D014508:Urea; C008247:laminaran; D000135:Acid Phosphatase; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D043324:beta-Fructofuranosidase; D043326:Glucan 1,3-beta-Glucosidase