Epidermal and hepatic glucocorticoid receptors in cats and dogs.
Low capacity, high affinity [3H] dexamethasone binding receptors were identified in cytosolic preparations of liver (mean number 45 +/- 10.1 fmol mg-1 protein, apparent dissociation constant 0.4 +/- 0.1 nM) and skin (mean number 46.4 +/- 23.8 fmol mg-1 protein, apparent dissociation constant 1 +/- 0.2 nM) of clinically normal dogs. For clinically normal cats, approximately half these numbers of receptors with a lower affinity, were detected in liver (mean number 23.1 +/- 10.4 fmol mg-1 protein, apparent dissociation constant 3.2 +/- 0.9 nM) and skin (mean number 23.90 +/- 10.9 fmol mg-1 protein, apparent dissociation constant 2.2 +/- 1.5 nM). This difference between dogs and cats in [3H] dexamethasone binding receptors may contribute to the relative glucocorticoid resistance observed in cats.
van den Broek
A H
AH
Department of Veterinary Clinical Studies, University of Edinburgh, Royal (Dick) School of Veterinary Studies, Summerhall.
Stafford
W L
WL
D000818:Animals; D002415:Cats / Q000378:metabolism*; D003907:Dexamethasone / Q000378:metabolism; D004285:Dogs / Q000378:metabolism*; D004817:Epidermis / Q000737:chemistry / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000032:analysis*
D011965:Receptors, Glucocorticoid; C028697:dexamethasone receptor; D003907:Dexamethasone