Crystallization and preliminary X-ray study of minor glucoamylase from Aspergillus awamori variant X-100/D27.
Crystals of the reduced form of glucoamylase were obtained from polyethylene glycol 6000 solution by the hanging-drop method. The protein was treated with alpha-mannosidase to partly remove the sugar component. The crystals belong to the space group P2(1)2(1)2(1) with cell dimensions a = 116.7 A, b = 104.3 A, c = 48.5 A and diffract beyond 2.5 A resolution.
Golubev
A M
AM
Leningrad Nuclear Physics Institute, U.S.S.R. Academy of Sciences, Gatchina.
Neustroev
K N
KN
Aleshin
A E
AE
Firsov
L M
LM
D001230:Aspergillus / Q000201:enzymology*; D003460:Crystallization; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry* / Q000378:metabolism; D008361:Mannosidases / Q000378:metabolism; D011092:Polyethylene Glycols; D014961:X-Ray Diffraction; D043323:alpha-Mannosidase
D011092:Polyethylene Glycols; D008361:Mannosidases; D043323:alpha-Mannosidase; D005087:Glucan 1,4-alpha-Glucosidase