title: Parameters affecting the adsorption of ligands to polyvinyl chloride plates in enzyme immunoassays.
abstract: In the present work, we studied the efficacy of three blocking agents (HSA, BSA and OVA) in the inhibition of non-specific binding to PVC plates. According to the inhibition data, 1% OVA was the most effective blocking agent. On the other hand, the presence of detergents in all of the blocking solutions drastically decreased the percent inhibition of the non-specific binding. Furthermore, the effect of ligand concentration on adsorption and the kinetics of ligand adsorption to PVC plates were also investigated. Ligand adsorption is a linear function of input up to a limit (around 8.70 ng/mm2) where saturation is reached. The rate of adsorption of pure human IgG to PVC plates was proportionally increased with the temperature, as shown by proportional rate constants almost 2 times faster at 37 degrees C than at 4 degrees C. These results have practical implications for investigators using PVC for immunoassays and should be taken into consideration when designing such assays.
authors.affiliation: Departamento de Bioquimica Clínica, Facultad de Ciencias Quimicas, Universidad Nacional de Córdoba, Argentina.
authors.forename: F E
authors.initials: FE
authors.lastname: Zalazar
authors.forename: G A
authors.initials: GA
authors.lastname: Chiabrando
authors.forename: M A
authors.initials: MA
authors.lastname: Aldao
authors.forename: M A
authors.initials: MA
authors.lastname: Vides
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007164:Immunosorbents; D007700:Kinetics; D008024:Ligands; D010047:Ovalbumin / Q000737:chemistry; D011143:Polyvinyl Chloride; D012709:Serum Albumin / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions
chemical_list: D007164:Immunosorbents; D008024:Ligands; D012709:Serum Albumin; D012996:Solutions; D012710:Serum Albumin, Bovine; D011143:Polyvinyl Chloride; D010047:Ovalbumin