title: Postnatal development of corticosteroid receptor immunoreactivity in the rat cerebellum and brain stem.
abstract: The postnatal development of corticosteroid receptor immunoreactivity in the rat cerebellum and related brainstem nuclei was studied using a type I receptor antiserum, MINREC4, and a type II receptor monoclonal antibody, BUGR2. Type I receptor immunoreactive (ir) Purkinje cells were first observed at postnatal day 5 (P5), and increased to adult levels by P20. Type I-ir cells, presumably migrating granule cells, were observed in the developing molecular layer of the cerebellum at P5. By P30, the density of type I-ir cells in the definitive molecular and granular layers was still less than adult levels. In contrast, type II-ir Purkinje cells were first observed at P15 and increased to adult levels by P20. No type II-ir cells were observed in the proliferative and migratory zones of the molecular layer. By P30, the density of type II-ir cells in the molecular and granular layers was far less than adult levels. In the deep cerebellar nuclei and most brain stem nuclei type I-ir was observed at P5 and developed to adult levels by P30. Type II-ir was observed in the deep cerebellar nuclei, red and medial vestibular nuclei by P15. The pontine and inferior olivary nuclei showed type II-ir cells by P10. Type II-ir in these regions developed to adult levels by P30. The earlier development of type I-ir suggests that the type I receptor may mediate the actions of corticosteroids in the cerebellum and related brain stem nuclei during early postnatal life.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy, Tulane University School of Medicine, New Orleans, La.
authors.forename: A
authors.initials: A
authors.lastname: Lawson
authors.forename: R S
authors.initials: RS
authors.lastname: Ahima
authors.forename: Z
authors.initials: Z
authors.lastname: Krozowski
authors.forename: R E
authors.initials: RE
authors.lastname: Harlan
mesh_terms: D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D001933:Brain Stem / Q000737:chemistry / Q000254:growth & development*; D002531:Cerebellum / Q000737:chemistry / Q000254:growth & development*; D007124:Immunoenzyme Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000032:analysis*
chemical_list: D011965:Receptors, Glucocorticoid