Adenovirus helper function activity of simian virus 40 T antigen mutants.
The SV40 large T antigen provides a helper function that permits human adenovirus yields in monkey cells to approach those obtained in human cells. The carboxy-terminus of large T antigen is involved in providing this activity. The ability of a large number of SV40 mutants affecting T antigen to enhance the growth of adenovirus type 2 in the CV-1 line of African green monkey kidney cells was examined. Mutation of those serines and threonines at the carboxy terminus which are normally phosphorylated had no effect on adenovirus helper function. A cytoplasmic T antigen was very effective in providing adenovirus helper function. Mutants that produce unstable T antigens provided helper function, but to a reduced degree. Finally, mutations in T antigen which permit it to interfere trans-dominantly with replication catalyzed by wildtype T antigen provided adenovirus helper function at wildtype levels.
Heath
C V
CV
Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755.
Fanning
E
E
Cole
C N
CN
D000260:Adenoviruses, Human / Q000235:genetics* / Q000254:growth & development; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002460:Cell Line; D002522:Chlorocebus aethiops; D004252:DNA Mutational Analysis; D015967:Gene Expression Regulation, Viral; D006801:Humans; D066298:In Vitro Techniques; D009154:Mutation; D014162:Transfection; D010948:Viral Plaque Assay; D014779:Virus Replication
D000952:Antigens, Polyomavirus Transforming