title: Sodium butyrate selectively induces transcription of promoters adjacent to the MoMSV viral enhancer.
abstract: The long terminal repeat region of the Moloney murine sarcoma virus (MoMSV) was cloned upstream from the Chinese hamster ovary adenine phosphoribosyltransferase (APRT)-encoding gene (APRT) in order to enhance synthesis of the APRT protein. The replacement of the native promoter with the viral enhancer-promoter increased the enzymatic activity of APRT two- to threefold. Addition of sodium butyrate (NaBu) to the cell growth medium induced APRT activity ten- to 20-fold above wild-type levels in both transient and stable transfectants. The introduction of the APRT native promoter between the MoMSV enhancer-promoter and structural gene reduced the magnitude of the NaBu response. The bacterial cat gene was also stimulated by NaBu when linked to the viral enhancer-promoter. No NaBu response was found in constructs lacking the MoMSV enhancer region. Northern analysis and nuclear run-on experiments indicated that NaBu enhanced transcription of APRT mRNA in both transiently and stably transfected cells, but not in cells inhibited by cycloheximide. Thus, a butyrate-response element (BRE) is associated with the MoMSV enhancer and the action of the MoMSV BRE is promoter-dependent.
authors.affiliation: Department of Biology, Indiana University, Bloomington 47405.
authors.forename: A
authors.initials: A
authors.lastname: Yeivin
authors.forename: D C
authors.initials: DC
authors.lastname: Tang
authors.forename: M W
authors.initials: MW
authors.lastname: Taylor
mesh_terms: D000228:Adenine Phosphoribosyltransferase / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D006224:Cricetinae; D004742:Enhancer Elements, Genetic / Q000235:genetics; D008980:Moloney murine sarcoma virus / Q000187:drug effects* / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D012333:RNA, Messenger / Q000187:drug effects; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection
chemical_list: D002087:Butyrates; D012333:RNA, Messenger; D020148:Butyric Acid; D000228:Adenine Phosphoribosyltransferase