Expression of calbindin-D28k in developing and growing chick testes.
Calbindin, a 28-kDa vitamin D-dependent calcium-binding protein was localized immunohistochemically in developing and growing chick testes. The protein first appeared in the germinal epithelium of developing testes of the eight-day-old embryo and remained therein throughout development. Calbindin was not present in the germinal epithelium after hatching. Calbindin was next detected in the spermatogonia and spermatocytes of one-week-old and growing chick testes. Calbindin-positive spermatogonia and spermatocytes gradually increased in number and staining intensity as the seminiferous tubules further developed. A few interstitial Leydig cells were positive for calbindin from five-week-old and older chicks. Comparison of the time-course of appearance and increase in calbindin content in spermatogonia and spermatocytes with spermatogenesis in chickens suggests that calbindin may be involved in the mitotic process in spermatogonia and spermatocytes.
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Department of Veterinary Anatomy and Cellular Biology OSU, Columbus, OH 43210.
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D000818:Animals; D064026:Calbindins; D002642:Chick Embryo; D002645:Chickens; D007150:Immunohistochemistry; D007985:Leydig Cells / Q000737:chemistry*; D008297:Male; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D013090:Spermatocytes / Q000737:chemistry*; D013093:Spermatogonia / Q000737:chemistry*; D013737:Testis / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development
D064026:Calbindins; D064030:S100 Calcium Binding Protein G