title: Large scale extraction of alpha-lactalbumin and beta-lactoglobulin from bovine whey by precipitation with polyethylene glycol and partitioning in aqueous two-phase systems.
abstract: The milk proteins alpha-lactalbumin and beta-lactoglobulin have been isolated from bovine whey by fractional precipitation with polyethylene glycol (PEG) and hydrophobic partitioning in aqueous PEG-hydroxypropylstarch two-phase systems using PEG-bound palmitate as hydrophobic ligand. The possible use of this combination for large scale purification of these whey proteins is discussed.
authors.affiliation: Department of Biochemistry, University of Lund, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Ortín
authors.forename: J A
authors.initials: JA
authors.lastname: Cebrian
authors.forename: G
authors.initials: G
authors.lastname: Johansson
mesh_terms: D000818:Animals; D002417:Cattle; D005069:Evaluation Studies as Topic; D005590:Fractional Precipitation; D007768:Lactalbumin / Q000737:chemistry / Q000302:isolation & purification*; D011092:Polyethylene Glycols; D012995:Solubility; D013213:Starch / Q000031:analogs & derivatives
chemical_list: D011092:Polyethylene Glycols; D013213:Starch; D007768:Lactalbumin; C046042:2-hydroxypropyl starch