Continuous monitoring of analyte concentrations.
We have investigated the application of a modified, heterogeneous, competitive enzyme immunoassay for the continuous measurement of small analytes in a medium stream. The analytical system contains two antibodies that are immobilized on spatially separated areas, one binding the analyte (Ab1) and the other binding the enzyme (Ab2). An analyte-enzyme conjugate serves as signal generator. The analyte-enzyme conjugate functions as a heterobifunctional shuttle that can bind to either antibody. A semipermeable membrane retains the enzyme shuttle in the internal volume of the sensor but permits the passage of small analytes from the medium stream. The amount of enzyme bound to Ab1 is inversely proportional and the amount of enzyme bound to Ab2 is directly proportional to the analyte concentration. We have demonstrated that this analytical system (1) can provide a larger total signal; (2) has a sensitivity comparable with conventional competitive immunoassays; (3) does not require the separation of bound from free antigens; and (4) is therefore suitable for the continuous measurement of analytes in a medium stream. With a model system, an increase from 0 ng ml-1 to 20 ng ml-1 of the steroid hormone progesterone and the subsequent fall to 0 ng ml-1 could be monitored.
Schramm
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Reproductive Sciences Program, University of Michigan, Ann Arbor 48109.
Paek
S H
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D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D006735:Horseradish Peroxidase / Q000737:chemistry; D007124:Immunoenzyme Techniques; D007700:Kinetics; D011374:Progesterone / Q000737:chemistry
D000911:Antibodies, Monoclonal; D011374:Progesterone; D006735:Horseradish Peroxidase