Molecular expression of the negative growth factor murine beta-galactoside binding protein (mGBP).
Characterisation of the negative growth factor mGBP at molecular and biological levels indicates that the protein has no lectin nature and suggests instead a participation in the cytokine network. The protein is shown to be expressed as a monomer in two forms, one of which is non-covalently linked to a glycan complex. This confers greater efficiency to the inhibitor and may favour a paracrine role. The two monomeric forms may oxidise into tetramers which retain biological activity, but lack ability to link to specific saccharide residues.
Wells
V
V
Laboratory of Cellular and Molecular Biology, United Medical School, Guy's Hospital, London Bridge, UK.
Mallucci
L
L
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005697:Galactosides / Q000378:metabolism*; D037161:Galectins; D015870:Gene Expression; D006388:Hemagglutinins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000032:analysis; D011487:Protein Conformation; D014162:Transfection
D005697:Galactosides; D037161:Galectins; D006388:Hemagglutinins; D011134:Polysaccharides; C007816:beta-galactoside