Use of perfluorocarbon emulsions in cell culture.
Perfluorocarbon emulsions were applied to hybridoma cultures grown in tissue culture tubes and column bioreactors. The oxygen transfer enhancement effect of perfluorocarbon emulsions was clearly demonstrated by the higher cell densities obtained in emulsion-supplemented systems. In addition, perfluorocarbon emulsions were shown to provide better cell suspension in a low-shear environment. The study in column bioreactors also suggested a cell protective effect of the employed perfluorocarbon emulsions in reducing the damage to cells by gas bubbles.
Ju
L K
LK
Department of Chemical Engineering, The University of Akron, OH 44325.
Armiger
W B
WB
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D004655:Emulsions; D005466:Fluorocarbons; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism; D051379:Mice; D010100:Oxygen / Q000378:metabolism
D003470:Culture Media; D004655:Emulsions; D005466:Fluorocarbons; D010100:Oxygen