Monoclonal antibody mediated targeting of enzymes. A comparative study using the mouse spleen as a model system.
The aim of this study was to target enzymes specifically to cells in the murine spleen. Monomeric and polymeric conjugates of the enzymes horseradish peroxidase or alkaline phosphatase and monoclonal antibodies against cell surface determinants were prepared. Highly specific in vivo targeting of enzymes to macrophages was obtained only when monomeric MAb-enzyme conjugates were used.
van Rooijen
N
N
Department of Cell Biology, Medical Faculty, Vrije Universiteit, Amsterdam, Netherlands.
ter Hart
H
H
Kraal
G
G
Kors
N
N
Claassen
E
E
D000469:Alkaline Phosphatase / Q000008:administration & dosage / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D004798:Enzymes / Q000008:administration & dosage* / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000008:administration & dosage / Q000737:chemistry; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D013154:Spleen / Q000276:immunology; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D004798:Enzymes; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase