The relation between the synergistic effect of cytostatics and metabolism of V 79B cells in vitro.
A single-dose simultaneous application of methotrexate (MTX; 0.002/microgram ml-1) and cisplatin (cis-Pt; 0.0002/microgram ml-1) had a permanent synergistic effect on both synchronized and asynchronous cell populations of V 79B cells. Successive combination of the drugs was manifested synergistically when MTX was applied first. The synchronized cell population was more sensitive to the cytostatics than the asynchronous population. Treatment with MTX alone, or the combination of MTX-cis-Pt, as well as their successive combination with the first drug being cis-Pt, caused gluconeogenesis.
Hudáková
A
A
Faculty of Natural Sciences, Comenius University, Bratislava, Czechoslovakia.
Horáková
K
K
Perényi
T
T
D000818:Animals; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology*; D006224:Cricetinae; D004357:Drug Synergism; D005943:Gluconeogenesis / Q000187:drug effects; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008727:Methotrexate / Q000494:pharmacology*
D002945:Cisplatin; D008727:Methotrexate