Structure of a Micrococcus lysodeikticus cell wall fragment containing phosphorylated sugars.
A polysaccharide-peptidoglycan complex containing different phosphorylated sugars from Micrococcus lysodeikticus cell wall has been isolated and purified. The peptidoglycan contained muramic acid 6-phosphate and N-acetylglucosamine 6-phosphate as phosphorylated sugars in addition to other sugar residues. Mild acid hydrolysis of the peptidoglycan and subsequent reduction of the released polysaccharide showed therein the presence of glucose and N-acetyl-glucosamine in the linkage of the external polysaccharide residues to the peptidoglycan through phosphodiester linkage. These data suggest the presence of polysaccharide chains linked to a peptidoglycan core through two phosphorylated sugars via two different terminal carbohydrate residues of the external polysaccharide chains in a same polymer.
Siddiqui
M
M
Institute of Biochemistry, University of Balochistan, Quetta, Pakistan.
Qureshi
M H
MH
Malghani
M A
MA
Walker-Nasir
E
E
Fayyaz-ud-Din
Nasir-ud-Din
D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D007700:Kinetics; D008837:Micrococcus / Q000737:chemistry* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010457:Peptidoglycan / Q000378:metabolism; D010766:Phosphorylation; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D010457:Peptidoglycan; D011135:Polysaccharides, Bacterial