title: Sensitive and specific polymerase chain reaction assays for diagnosis of human T-cell lymphotropic virus type I (HTLV-I) and HTLV-II infections in HTLV-I/II-seropositive individuals.
abstract: To confirm and differentiate between human T-cell lymphotropic virus type I (HTLV-I) and HTLV-II infections, we analyzed by polymerase chain reaction (PCR) samples of peripheral blood lymphocytes from 98 individuals seropositive for HTLV-I/II using pol (SK110/111) and tax (SK43/44) consensus primer pairs. A total of 96 samples (97.9%) were positive by the tax generic probe, while 95 were typed by the HTLV-I and HTLV-II pol probes. The three pol-negative samples were successfully amplified and typed by nested PCR with primers internal to SK110 and SK111. Results of PCR with a lysate of leukocyte nuclei obtained by whole blood lysis were comparable to those obtained with peripheral blood lymphocytes from 16 HTLV-seropositive subjects.
authors.affiliation: Retrovirus Disease Branch, Centers for Disease Control, Atlanta, Georgia 30333.
authors.forename: W
authors.initials: W
authors.lastname: Heneine
authors.forename: R F
authors.initials: RF
authors.lastname: Khabbaz
authors.forename: R B
authors.initials: RB
authors.lastname: Lal
authors.forename: J E
authors.initials: JE
authors.lastname: Kaplan
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D015750:Genes, gag; D016355:Genes, pX; D015751:Genes, pol; D015490:HTLV-I Infections / Q000175:diagnosis*; D015491:HTLV-II Infections / Q000175:diagnosis*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D015367:Human T-lymphotropic virus 2 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral