m-[125I]iodoaniline: a useful reagent for radiolabeling biotin.
Biotinyl-m-[125I]iodoanilide (BIA) was synthesized by coupling biotin to m-[125I]iodoaniline via a mixed anhydride reaction. m-[125I]Iodoaniline was produced from the tin precursor, which was prepared using a palladium catalyzed reaction of hexabutylditin with m-bromoaniline. The radioiodinated BIA derivative is characterized by a stable amide and/or intact ureido group on the biotin molecule; it may thus be a useful carrier for targeting radionuclides to avidin-conjugated antibodies previously localized on tumors.
Khawli
L A
LA
Department of Radiology (Nuclear Medicine), Harvard Medical School, Shields Warren Radiation Laboratory, Boston, MA 02115.
Kassis
A I
AI
D000814:Aniline Compounds / Q000737:chemistry*; D001360:Avidin / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D001710:Biotin / Q000737:chemistry*; D007457:Iodine Radioisotopes / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods*; D019809:Streptavidin
D000814:Aniline Compounds; D001426:Bacterial Proteins; D007457:Iodine Radioisotopes; D001360:Avidin; C075716:3-bromoaniline; C075715:3-iodoaniline; D001710:Biotin; D019809:Streptavidin