A reference preparation of human prostatic acid phosphatase: purification, characterization and field trials.
Acid phosphatase has been prepared in an apparently pure state by affinity chromatography from human prostatic tissue. When dissolved in an acidic albumin solution, lyophilized and stored at -20 degrees C for up to 2 years, no time-dependent loss of catalytic activity was detectable in the reconstituted material. Accelerated degradation tests also predicted complete stability. A preliminary distribution of the lyophilized preparation to 143 laboratories confirmed its robustness and demonstrated its potential usefulness as a calibrant to unify the results of different methods of measuring acid phosphatase activity.
Francis
J M
JM
Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Moss
D W
DW
Colinet
E
E
Calam
D H
DH
Bullock
D G
DG
D000135:Acid Phosphatase / Q000302:isolation & purification* / Q000592:standards; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D008297:Male; D011467:Prostate / Q000201:enzymology*; D011506:Proteins / Q000032:analysis; D012016:Reference Values
D011506:Proteins; D000135:Acid Phosphatase