Expression of c-fos, c-jun, jun-B, metallothionein and metalloproteinase genes in human chondrocyte.
Normal and osteoarthritic (OA) human articular cartilage chondrocytes, released enzymatically in the presence of 0.5% fetal calf serum, display constitutive expression of early response activating protein (AP-1) genes; c-fos, c-jun and jun-B. Among the late AP-1 responsive genes, total metallothionein (MT) and stromelysin mRNAs were expressed at high levels in both normal and OA chondrocytes, while collagenase and hMT-IIA mRNA levels were elevated only in OA individuals. Despite the common AP-1 sequences present in their promoter regions, the three late genes were differentially expressed.
Zafarullah
M
M
Notre-Dame Hospital Research Centre, Department of Medicine, University of Montreal, Qué., Canada.
Martel-Pelletier
J
J
Cloutier
J M
JM
Gedamu
L
L
Pelletier
J P
JP
D000368:Aged; D015152:Blotting, Northern; D002356:Cartilage / Q000166:cytology / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008666:Metalloendopeptidases / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics*; D010003:Osteoarthritis / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*
D004268:DNA-Binding Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D008668:Metallothionein; D008666:Metalloendopeptidases