Large scale extraction of alpha-lactalbumin and beta-lactoglobulin from bovine whey by precipitation with polyethylene glycol and partitioning in aqueous two-phase systems.
The milk proteins alpha-lactalbumin and beta-lactoglobulin have been isolated from bovine whey by fractional precipitation with polyethylene glycol (PEG) and hydrophobic partitioning in aqueous PEG-hydroxypropylstarch two-phase systems using PEG-bound palmitate as hydrophobic ligand. The possible use of this combination for large scale purification of these whey proteins is discussed.
Ortín
A
A
Department of Biochemistry, University of Lund, Sweden.
Cebrian
J A
JA
Johansson
G
G
D000818:Animals; D002417:Cattle; D005069:Evaluation Studies as Topic; D005590:Fractional Precipitation; D007768:Lactalbumin / Q000737:chemistry / Q000302:isolation & purification*; D011092:Polyethylene Glycols; D012995:Solubility; D013213:Starch / Q000031:analogs & derivatives
D011092:Polyethylene Glycols; D013213:Starch; D007768:Lactalbumin; C046042:2-hydroxypropyl starch