Liquid chromatographic determination of hyoscine (scopolamine) in urine using solid phase extraction.
A sensitive method for the determination of hyoscine (scopolamine) in urine is described. After concentration and "clean-up" on C18 and CN solid phase extraction columns, hyoscine was quantified by high performance liquid chromatography with coulometric detection (oxidation at +0.9 V). The limit of detection was 5 ng per sample and the precision for 5 mL samples containing 2 ng/mL was 12.3%. The method was applied to urine samples collected from 12 volunteers wearing Scopoderm TTS patches. The mean excretion rate of unmetabolized hyoscine was 0.45 micrograms/h and 87% of the total hyoscine was present as conjugates. Apohyoscine (aposcopolamine) was identified as a urinary metabolite. The significance of this with regard to hyoscine assays is discussed.
Whelpton
R
R
Department of Pharmacology, Queen Mary and Westfield College, London, UK.
Hurst
P R
PR
Metcalfe
R F
RF
Saunders
S A
SA
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004563:Electrochemistry; D006801:Humans; D006868:Hydrolysis; D008297:Male; D012016:Reference Values; D012601:Scopolamine / Q000652:urine*
D012601:Scopolamine