title: Modified silver staining for immobilized pH gradients.
abstract: Silver development of gels containing an immobilized pH gradient has proved difficult so far because the bonded buffers (especially the tertiary amino acrylamido derivatives) tend to absorb silver ions with a resultant heavy background of increasing darkness from the anode to the cathode. We report a variant of silver staining in which thiosulfate is used twice: (i) prior to silver impregnation, at the millimolar level, to enhance sensitivity, and (ii) during development, at the micromolar level, to decrease the background.
authors.affiliation: Unité Génétique et Biochimie du Développement, Institut Pasteur, Paris, France.
authors.forename: T
authors.initials: T
authors.lastname: Rabilloud
authors.forename: V
authors.initials: V
authors.lastname: Brodard
authors.forename: G
authors.initials: G
authors.lastname: Peltre
authors.forename: P G
authors.initials: PG
authors.lastname: Righetti
authors.forename: C
authors.initials: C
authors.lastname: Ettori
mesh_terms: D006863:Hydrogen-Ion Concentration; D016622:Silver Staining / Q000379:methods*; D013885:Thiosulfates
chemical_list: D013885:Thiosulfates