Variation in coding exons of two electrophoretic alleles at the pigtail macaque carbonic anhydrase I locus as determined by direct, double-stranded sequencing of polymerase chain reaction (PCR) products.
Two, electrophoretically distinct, forms of carbonic anhydrase I (CA Ia and CA Ib) are found at high polymorphic frequencies in red cells of natural populations of pigtail macaques, Macaca nemestrina, from southeast Asia. By use of the polymerase chain reaction, exons of the CA I gene were amplified from homozygous (a/a, b/b) and heterozygous (a/b) animals. Direct sequencing of the amplified DNA from four animals revealed differences between the a and the b electrophoretic alleles ranging from three to six nucleotides, and from one to three differences within each allele. These results indicate a greater genetic variability at the CA I locus in this macaque species than previously realized.
Bergenhem
N C
NC
Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109-0618.
Venta
P J
PJ
Hopkins
P J
PJ
Tashian
R E
RE
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002256:Carbonic Anhydrases / Q000235:genetics*; D002874:Chromosome Mapping; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D014644:Genetic Variation; D006579:Heterozygote; D006720:Homozygote; D007527:Isoenzymes / Q000235:genetics*; D008254:Macaca nemestrina / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D007527:Isoenzymes; D002256:Carbonic Anhydrases