title: Determination of 8-methoxypsoralen in plasma by gas chromatography-mass spectrometry using selected-ion monitoring.
abstract: A sensitive and accurate assay was developed for the measurement of 8-methoxypsoralen in plasma using electron-impact positive-ion mass fragmentography. 4,5,8-Trimethylpsoralen was used as an internal standard. Sample preparation consisted of a two-step liquid phase extraction using acetonitrile and methylene chloride. The calibration curve showed a linear relationship between the peak areas of 8-methoxypsoralen and 4,5,8-trimethylpsoralen over a wide range of 8-methoxypsoralen concentrations (1-500 ng/ml). With-in- and between-run precisions, measured at five different drug concentrations, varied from 0.82 to 1.41% and from 0.82 to 1.86%, respectively.
authors.affiliation: Department of Pediatrics, University of Padua, Italy.
authors.forename: A T
authors.initials: AT
authors.lastname: Cracco
authors.forename: R
authors.initials: R
authors.lastname: Dall'Amico
authors.forename: V
authors.initials: V
authors.lastname: Ferrari
authors.forename: S
authors.initials: S
authors.lastname: Bottaro
authors.forename: G
authors.initials: G
authors.lastname: Zacchello
authors.forename: L
authors.initials: L
authors.lastname: Chiandetti
authors.forename: F
authors.initials: F
authors.lastname: Zacchello
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007477:Ions; D008730:Methoxsalen / Q000378:metabolism* / Q000627:therapeutic use; D010778:Photochemotherapy
chemical_list: D007477:Ions; D008730:Methoxsalen