[Separation of urologic tumors cells from Cell Saver blood using a membrane filter. A new method in autotransfusion?].
Tumor cells of three urological longterm cell lines have been labelled with 35[S]-Methionin and added to red cell concentrates. Red cell concentrates rich in tumor cells were passed through a cell saver and two special membranfilters under standard conditions. The 35[S]-Methionin labelled tumor cells were detected by liquid scintillation counting. On an average, only 0.027% of the radioactivity was left after passing through the cell saver and the membran filters in the 12 experiments. If investigations in clinical use of cell-saver and membran filter confirm these results, there will be significant consequences in urological tumor surgery by the possibility of transfusing the salvaged autologous blood.
Wiesel
M
M
Abteilung für Urologie, Klinikum der Universität, Heidelberg.
Güdemann
C
C
Staehler
G
G
Bierhaus
A
A
Martin
E
E
Hoever
K H
KH
D001781:Blood Component Removal / Q000295:instrumentation*; D016913:Blood Component Transfusion / Q000295:instrumentation; D001804:Blood Transfusion, Autologous / Q000295:instrumentation*; D002292:Carcinoma, Renal Cell / Q000097:blood; D002295:Carcinoma, Transitional Cell / Q000097:blood; D002460:Cell Line; D002469:Cell Separation / Q000295:instrumentation*; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood; D008297:Male; D009360:Neoplastic Cells, Circulating; D011471:Prostatic Neoplasms / Q000097:blood; D001749:Urinary Bladder Neoplasms / Q000097:blood; D014571:Urologic Neoplasms / Q000097:blood*
Separation von urologischen Tumorzellen aus Cell-Saver-Blut durch Einsatz eines Membranfilters. Ein neuer Weg in der Autotransfusion?