Biological activity of bovine placental lactogen in 3T3-F442A preadipocytes is mediated through a somatogenic receptor.
Bovine placental lactogen (bPL) exhibited antimitogenic differentiation-promoting biological activity in 3T3-F442A preadipocytes. Competitive binding studies and affinity labelling revealed bPL activity to be mediated through a somatogenic type of receptor that recognizes human growth hormone (hGH) and bovine GH, but not ovine prolactin or human PL. The bioactivity of bPL was sixfold lower than that of hGH despite that bPL is binding to the somatogenic receptors with fivefold higher affinity. This discrepancy may result from the relatively low ability of bPL to induce post-receptoral effects such as receptor dimerization.
Vashdi
D
D
Department of Biochemistry and Human Nutrition, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Elberg
G
G
Sakal
E
E
Gertler
A
A
D016475:3T3 Cells; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000345:Affinity Labels; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology; D051379:Mice; D008934:Mitogens / Q000037:antagonists & inhibitors; D010928:Placental Lactogen / Q000494:pharmacology*; D011388:Prolactin / Q000494:pharmacology; D011869:Radioligand Assay; D011986:Receptors, Somatotropin / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000345:Affinity Labels; D008934:Mitogens; D011986:Receptors, Somatotropin; D011994:Recombinant Proteins; D011388:Prolactin; D013006:Growth Hormone; D010928:Placental Lactogen