title: Purification and characterization of beta-galactoside-binding proteins from Caenorhabditis elegans.
abstract: Two carbohydrate-binding proteins (subunit molecular masses, 32 and 16 kDa, respectively) were isolated for the first time from a nematode, Caenorhabditis elegans. They were specifically extracted with lactose and adsorbed on asialofetuin-Sepharose in the absence of a metal ion. Although these two proteins were co-eluted from a gel filtration column at a position corresponding to an apparent molecular size of 30 kDa under non-denaturing conditions, they could be separated by reversed-phase chromatography. The 32 kDa protein, the main component, was further characterized. Together with its solubility, saccharide specificity and metal independence, some other structural properties, including its amino acid composition, UV spectrum, and partial amino acid sequence, strongly suggested that the 32 kDa protein is a member of a class of soluble beta-galactoside-binding lectins which had previously been only found in vertebrates.
authors.affiliation: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa.
authors.forename: J
authors.initials: J
authors.lastname: Hirabayashi
authors.forename: M
authors.initials: M
authors.lastname: Satoh
authors.forename: Y
authors.initials: Y
authors.lastname: Ohyama
authors.forename: K
authors.initials: K
authors.lastname: Kasai
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002107:Caenorhabditis / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D005697:Galactosides / Q000378:metabolism; D037161:Galectins; D006388:Hemagglutinins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D008970:Molecular Weight; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D002352:Carrier Proteins; D005697:Galactosides; D037161:Galectins; D006388:Hemagglutinins