Monoclonal antibodies bind identically to both spores and hyphae of Aspergillus fumigatus.
Immunoelectron microscopy (IEM) was used to determine the binding of six monoclonal antibodies (MoAbs) produced against Aspergillus fumigatus antigens present on or within the conidia and hyphae of the fungus. Antigen-antibody complexes were demonstrated in EM using labelled colloidal gold particles (15 nm). Three out of 6 MoAbs (C9, F12 and H10) reacted only with the cytoplasmic components of A. fumigatus while the remaining three (B12, F6G5 and D6E6) showed reactivity to both cytoplasm and cell wall of the conidia and hyphae. The results indicate that IEM is of considerable value in determining and selecting monoclonal antibodies having specific reactivity with diverse antigenic components.
Reijula
K E
KE
Research Service, VA Medical Center, Milwaukee, WI.
Kurup
V P
VP
Kumar
A
A
Fink
J N
JN
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000946:Antigens, Fungal; D001228:Aspergillosis / Q000276:immunology; D001232:Aspergillus fumigatus / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D002473:Cell Wall / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology; D006801:Humans; D016253:Microscopy, Immunoelectron; D013172:Spores, Fungal / Q000276:immunology
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal