Virulence phenotypes of Shigella flexneri 2a avirulent mutant 24570 can be complemented by the plasmid-coded positive regulator virF gene.
Avirulent mutation of an opaque colony variant of Shigella flexneri 2a designated 24570 has been believed to be linked with the glpK locus of the chromosome. However, avirulent phenotypes of the 24570 strain could be complemented by the invasion plasmid-coded virF gene, a positive regulator for invasion genes. The 24570 strain had a DNA structural alteration upstream of the virF gene.
Qi
M S
MS
Department of Bacteriology, National Institute of Health, Tokyo, Japan.
Yoshikura
H
H
Watanabe
H
H
D000942:Antigens, Bacterial / Q000032:analysis; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012703:Serotyping; D012763:Shigella flexneri / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D000942:Antigens, Bacterial; D004269:DNA, Bacterial