title: An altered 11-kilobase transcript in leukemic cell lines with the t(4;11)(q21;q23) chromosome translocation.
abstract: The 11q23 chromosome band is frequently associated with chromosomal aberrations in human leukemias. We have previously cloned a DNA fragment derived from chromosome 11 which could be used as a probe to detect rearrangements in DNAs from the leukemic cells of patients with the t(4;11), t(9;11), and t(11;19) translocations. In this study we now show that the same probe detects DNA rearrangements in malignant cells from patients with the t(1;11), t(6;11), t(10;11), and del (11q23) chromosomal abnormalities. A second probe obtained from a region located centrometric to the breakpoint cluster detects major and minor transcripts of 12.5 and 11.5 kilobases, respectively, in all cell lines examined. The same probe identifies an altered 11-kilobase RNA in all three independent cell lines with the t(4;11)(q21;q23) chromosome translocation.
authors.affiliation: Jefferson Cancer Institute, Jefferson Cancer Center, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
authors.forename: G
authors.initials: G
authors.lastname: Cimino
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Gu
authors.forename: O
authors.initials: O
authors.lastname: Canaani
authors.forename: R
authors.initials: R
authors.lastname: Prasad
authors.forename: W M
authors.initials: WM
authors.lastname: Crist
authors.forename: A J
authors.initials: AJ
authors.lastname: Carroll
authors.forename: M
authors.initials: M
authors.lastname: Baer
authors.forename: C D
authors.initials: CD
authors.lastname: Bloomfield
authors.forename: P C
authors.initials: PC
authors.lastname: Nowell
mesh_terms: D002880:Chromosomes, Human, Pair 11; D002894:Chromosomes, Human, Pair 4; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D014158:Transcription, Genetic; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured