Reorganization of the cytoskeleton and morphological changes induced by 1,25-dihydroxyvitamin D3 in C3H/10T1/2 mouse embryo fibroblasts: relation to inhibition of proliferation.
The effects of 1,25-dihydroxyvitamin D3 (1,25(OH)2 D3) on cell morphology, the cytoskeleton, and fibronectin were studied in three lines of C3H/10T1/2 mouse embryo fibroblasts in which the antiproliferative effect of the hormone had previously been investigated. We showed that 1,25(OH)2D3 induced morphological changes in the nontransformed C3H/10T1/2 Cl 8 cells, which flattened and spread out markedly. Visualization of actin and tubulin by immunocytochemistry disclosed a reorganization of the microfilament and microtubular systems. 1,25(OH)2D3 also induced an increase in cell-surface-associated fibronectin. These changes were only slight in the transformed cell line C3H/10T1/2 Cl 16 and absent in the transformed C3H/10T1/2 TPA 482 cell line. These effects were correlated with the growth inhibition induced by the hormone, and this suggests a possible relationship between the 1,25(OH)2D3-induced alterations of cell shape and of the cytoskeleton and the effects of the hormone on cell proliferation.
Brackman
D
D
Department of Pediatrics, University of Bergen, Norway.
Trydal
T
T
Lillehaug
J R
JR
Aarskog
D
D
D008841:Actin Cytoskeleton / Q000187:drug effects; D000199:Actins / Q000737:chemistry; D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced; D005347:Fibroblasts; D005353:Fibronectins / Q000187:drug effects*; D051379:Mice; D008870:Microtubules / Q000187:drug effects; D010703:Phorbol Esters; D014404:Tubulin / Q000737:chemistry
D000199:Actins; D005353:Fibronectins; D010703:Phorbol Esters; D014404:Tubulin; D002117:Calcitriol