Phage T7 in biological UV dose measurement.
An experimental method complete with theoretical considerations is presented for the measurement of different biological UV doses. The method is based on the high sensitivity of phage T7 activity to UV light. A precisely determined T7 inactivation action spectrum is presented over a wide optical range (240-514 nm). Using the T7 spectral sensitivity in relation to the minimal erythema dose (MED) and the effective spectral irradiance from solar radiation for the MED, an example is given to determine the MED value based on the measurement of T7 inactivation for a given case. The advantages and applicability of the method are discussed.
Rontó
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Istitute of Biophysics, Semmelweis University of Medicine, Budapest, Hungary.
Gáspár
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Bérces
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D004307:Dose-Response Relationship, Radiation; D004890:Erythema / Q000209:etiology; D006801:Humans; D009381:Neoplasms, Radiation-Induced / Q000209:etiology; D012867:Skin / Q000528:radiation effects*; D012878:Skin Neoplasms / Q000209:etiology; D013472:Sunlight; D013604:T-Phages / Q000528:radiation effects*; D014466:Ultraviolet Rays