title: A transcriptional analysis of the gene encoding mouse U7 small nuclear RNA.
abstract: Expression of the U7 gene, encoding mouse U7 snRNA, following microinjection into Xenopus oocytes is both accurate and efficient, giving rise to mature U7 snRNA and a precursor with an 8-nucleotide (nt) 3' extension. The mouse U7 gene promoter, which is similar to that of the vertebrate major U genes comprising a DSE, a PSE and a 3' box, with the same spatial arrangement, is as efficient as the Xenopus U2 gene promoter in this assay. A deletion analysis of the mouse U7 gene identified sequences downstream from the 3' box, within the region (nt +74 to +196), which seem to have a negative regulatory effect upon the frequency of transcription initiation and are also required for accurate 3' end formation. Sequences in the nt -1699 to -431 region also seemed to have a negative effect on the level of transcription. In addition, sequences upstream from the PSE, within the nt -65 to -421 region, are necessary for accurate and efficient synthesis of mature U7 snRNA. Finally, the mouse U7 snRNA may not form a functional snRNP in Xenopus oocytes due to defective snRNP assembly and/or nuclear import.
authors.affiliation: Department of Biochemistry, University of Liverpool, UK.
authors.forename: S C
authors.initials: SC
authors.lastname: Phillips
authors.forename: P C
authors.initials: PC
authors.lastname: Turner
mesh_terms: D000818:Animals; D001483:Base Sequence; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D012342:RNA, Small Nuclear / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*; D014981:Xenopus / Q000235:genetics
chemical_list: D012342:RNA, Small Nuclear