title: Direct analysis of whole blood by internal surface reversed-phase chromatography: an examination of the binding and metabolism of technetium dioxime complexes.
abstract: We have developed a method using internal surface reversed-phase (ISRP) packing for rapid on-line separation of small hydrophobic compounds from cellular whole blood components. This is achieved by the use of 75-microns ISRP chromatographic material packed into a small high-performance liquid chromatographic (HPLC) column, in conjunction with column switching. We have applied this analytical method to study the in vitro metabolism of 99mTc-BATO (boronic acid adducts of technetium dioxime) cerebral and myocardial perfusion tracers in whole blood. The results from the ISRP procedure were compared with a conventional centrifugation method of analysis. This novel HPLC methods provides a rapid, convenient and reliable method for the analysis of radioactive and non-radioactive lipophilic components in whole blood.
authors.affiliation: Bristol-Myers Squibb Pharmaceutical Research Institute, New Brunswick, NJ 08903.
authors.forename: K C
authors.initials: KC
authors.lastname: Rosenspire
authors.forename: W
authors.initials: W
authors.lastname: Hirth
authors.forename: S
authors.initials: S
authors.lastname: Jurisson
authors.forename: D P
authors.initials: DP
authors.lastname: Nowotnik
authors.forename: W C
authors.initials: WC
authors.lastname: Eckelman
authors.forename: A D
authors.initials: AD
authors.lastname: Nunn
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008297:Male; D015609:Organotechnetium Compounds; D010091:Oximes; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D015609:Organotechnetium Compounds; D010091:Oximes