title: The interphase microtubule cytoskeleton of five different phenotypes of microvessel endothelial cell cultures derived from bovine corpus luteum.
abstract: The interphase microtubule cytoskeleton of five different microvessel endothelial cell cultures, recently established from bovine corpus luteum, was analysed using anti-tubulin immunofluorescence. An antibody against acetylated microtubules detected four cell types each of which possessed a single cilia. The length of the cilia were up to 10 microns for cell types 1 and 2. Ciliary stubs had a length of up to 0.37 microns in cell types 4 and 5. Cilia were missing in cell type 3. Long and short cilia were located in the perinuclear region from where cytoplasmic microtubules radiated. Cell type 3 displayed straight microtubules rather than the wavy path seen in the other cell types. The amount of tyrosinated microtubules visualized by a specific antibody was consistently higher than that of posttranslationally acetylated microtubules. The latter were more apparent in cell types 4 and 5 than in the other cell types. We conclude: Differences in the cytoplasmic microtubule inventory of each microvessel endothelial cell type points at individual functions maintained in culture.
authors.affiliation: Institut für Biologie, Medizinischen Universität zu Lübeck, Germany.
authors.forename: K W
authors.initials: KW
authors.lastname: Wolf
authors.forename: K
authors.initials: K
authors.lastname: Spanel-Borowski
mesh_terms: D000107:Acetylation; D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002478:Cells, Cultured; D002923:Cilia; D003338:Corpus Luteum / Q000098:blood supply*; D004730:Endothelium, Vascular / Q000737:chemistry*; D005260:Female; D007399:Interphase; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000737:chemistry*; D014443:Tyrosine
chemical_list: D000911:Antibodies, Monoclonal; D014443:Tyrosine