Incorporation of radioiodinated IPPA and BMIPP fatty acid analogues into complex lipids from isolated rat hearts.
Heart lipids were extracted by the Folch technique from Langendorff-perfused rat hearts after administration of 15-(p-[131I]iodophenyl)pentadecanoic acid and 15-(p-[125I]iodophenyl)-3-R,S-methylpentadecanoic acid. Techniques utilizing successive high performance liquid chromatographic (HPLC) analyses have been developed for the evaluation of the uptake of the tracers into neutral lipids and phospholipids of the rat hearts. Phospholipids were separated on a SiO2 column eluted with a gradient of acetonitrile/water (97.5/2.5) and acetonitrile/water (85/15) followed by separation of the neutral lipids on a C-18 reversed phase column with a gradient consisting of acetonitrile and 2-propanol/hexane (60/40) containing 1 N H2SO4 (5 microL/100 mL). Both tracers show the incorporation into the expected major lipid classes.
Kropp
J
J
Department of Nuclear Medicine and University of Bonn, Germany.
Ambrose
K R
KR
Knapp
F F
FF
Nissen
H P
HP
Biersack
H J
HJ
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007462:Iodobenzenes / Q000378:metabolism*; D007553:Isotope Labeling; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011916:Rats, Inbred F344
D005227:Fatty Acids; D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D008055:Lipids; C049338:iodofiltic acid; C025391:omega-(4-iodophenyl)pentadecanoic acid