Inhibition of HIV-1 infection in vitro by murine monoclonal anti-p24 antibodies.
Murine monoclonal antibodies (Mabs) to the major core protein p24 of the human immunodeficiency virus type 1 (HIV-1) were tested for their ability to inhibit the replication and spread of the virus in permanent cell cultures (Molt4/8, K37, H9) and in the culture of II-2 stimulated T cells of healthy donors. After addition of ascitic fluid containing monoclonal anti-p24 antibodies or purified anti-p24 antibodies or the respective control to co-cultures of infected and non-infected cells, HIV-1 replication was evaluated by determining the percentage of infected cells and the activity of reverse transcriptase (RT) in cell-free supernatant. In addition, the supernatant's infectivity was determined. FACS analysis demonstrated p24 antigen in about 40% of unfixed HIV-1 infected cells at the cell membrane. Monoclonal anti-p24 antibodies of different epitope specificity added to the cells but not to the virus delayed the spread of HIV-1 infection in permanent cell culture. Furthermore, anti-p24 Mabs inhibited the release of RT-active virus particles by HIV-1 infected cell lines or II-2 stimulated T-lymphocytes, respectively, up to 60%. The mode of action of anti-p24 antibodies after HIV-1 infection is discussed on the basis of the data obtained.
Franke
L
L
Department of Medical Immunology, Medical School (Charité), Humboldt University, Berlin, Germany.
Grunow
R
R
Meissner
K
K
Porstmann
T
T
von Baehr
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D002478:Cells, Cultured; D015483:HIV Antibodies / Q000008:administration & dosage; D016655:HIV Core Protein p24 / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D015497:HIV-1 / Q000276:immunology / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D013601:T-Lymphocytes / Q000382:microbiology; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D016655:HIV Core Protein p24