Facile cloning and sequencing of S-crystallin genes from octopus lenses based on polymerase chain reaction.
S-crystallin is a major lens protein present in the octopus and squid of Cephalopods. To facilitate the cloning of the protein, cDNA was constructed from the poly(A)+RNA of octopus lenses, and amplification by polymerase chain reaction (PCR) was carried out with two primers designed according to the 5'- and 3'-coding regions of S-crystallin gene. Sequencing two of 15 positive clones obtained shows 37-44% similarity in nucleotide and 23-30% similarity in amino acid sequences as compared with mammalian glutathione S-transferases (GST), revealing that S-crystallins exist as a multigene family and probably derived from GST by gene duplication and subsequent mutational base replacements.
Lin
C W
CW
Laboratory of Crystallin Research, National Taiwan University, Taipei.
Chiou
S H
SH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003459:Crystallins / Q000235:genetics*; D004247:DNA; D007908:Lens, Crystalline / Q000378:metabolism*; D008969:Molecular Sequence Data; D049831:Octopodiformes; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D003459:Crystallins; D004247:DNA