Pregnane derivatives as pregnancy interceptive agents: efficacy determination on growing trophoblasts (in vitro) and in pregnant hamsters (in vivo).
An in vitro test system was standardized to study potentiality of five hormonally inert pregnane derivatives on growing trophoblasts isolated from ectoplacental cone (EPC) of day 8 hamster embryo. Cells were incubated with different concentrations of respective compounds in surface droplets. The response was determined by analyzing the sequence of changes in cell morphology like attachment, growth, proliferation, differentiation and/or degeneration within 24 or 48 h following seeding. The in vivo efficacy of these compounds was determined in hamster during peri- and immediate post-implantation periods (days 3-8 post coitum). Two compounds 88/583 and 88/585 were found to inhibit not only growth and proliferation of the cells but caused total degeneration within 24 h. The same compounds induced partial to complete resorption of the foetuses in treated animals. Whereas, the other three compounds 88/506, 88/594 and 89/43 that showed lack of comparable potentiality in vitro were found to be equally ineffective in vivo. The results indicate a positive correlationship between in vitro and in vivo activity.
Shukla
R
R
Division of Endocrinology, Central Drug Research Institute, Lucknow, India.
Mehrotra
P K
PK
Dwivedi
A
A
Kamboj
V P
VP
D000021:Abortifacient Agents, Steroidal / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D005260:Female; D066298:In Vitro Techniques; D008647:Mesocricetus; D011247:Pregnancy; D011278:Pregnanes / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
D000021:Abortifacient Agents, Steroidal; D011278:Pregnanes