Reversed-phase high-performance liquid chromatographic tryptic digest peptide map comparisons of monoclonal antibodies to human tumor necrosis factor.
An automatic computer technique was used to compare the retention times and ultraviolet spectra of sixty-two peaks in peptide maps of three monoclonal antibodies against human tumor necrosis factor (TNF) and one monoclonal antibody against recombinant factor VIII. The anti-TNF monoclonal, B6, which has an overlapping epitope with the anti-TNF monoclonal, A10G10, had a 90% peak match with A10G10. The anti-TNF monoclonal, A6, with a different epitope to TNF than A10G10, had only a 60% peak match. The A6 match to A10G10 was similar to the 50% peak match of the anti-factor VIII monoclonal with A10G10. The results of this study suggest that peptide mapping can be used as a quantitative characterization technique for comparing monoclonal antibodies.
Bloom
J W
JW
Cutter Biological, Miles Inc., Berkeley, CA 94701.
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000918:Antibody Specificity; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010449:Peptide Mapping; D014357:Trypsin / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
D000911:Antibodies, Monoclonal; D014409:Tumor Necrosis Factor-alpha; D014357:Trypsin