title: A 9 A two-dimensional projected structure of cholera toxin B-subunit-GM1 complexes determined by electron crystallography.
abstract: Highly ordered two-dimensional crystals of cholera toxin B-subunit pentamers have been grown by specific interaction with planar lipid films containing monosialoganglioside GM1. Electron diffractograms of frozen-hydrated crystals show diffraction peaks extending to beyond 4 A, while electron images diffract to 8 A. A two-dimensional projected structure of cholera toxin B-subunit-GM1 complex has been calculated at 9 A resolution by combining electron diffraction and image data. Crystals present an approximate pgg projection symmetry, with unit cell dimensions a = 119(+/- 1) A, b = 123(+/- 1) A, gamma = 90 degrees. Each pentameric assembly presents two concentric rings of electron scattering density, separated by an area of lower density. The outer and inner rings are centered at 25 A and and 11 A from the pentamer centre, respectively. The apparent projected density of the outer ring is larger than that of the inner ring. We propose that the outer and inner density rings correspond respectively to the peripheral beta-sheet arrangement and the central alpha-helix barrel, recently identified in the crystal structure of the heat-labile enterotoxin from Escherichia coli.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique INSERM, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
authors.forename: G
authors.initials: G
authors.lastname: Mosser
authors.forename: V
authors.initials: V
authors.lastname: Mallouh
authors.forename: A
authors.initials: A
authors.lastname: Brisson
mesh_terms: D002772:Cholera Toxin / Q000737:chemistry / Q000378:metabolism*; D003460:Crystallization; D005677:G(M1) Ganglioside / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: D005677:G(M1) Ganglioside; D002772:Cholera Toxin