title: N-unsubstituted glucosamine residues and other modifications in murein of the obligatory chemolithotroph Thiobacillus neapolitanus.
abstract: Purified murein from Thiobacillus neapolitanus was poorly digested by lysozyme. It's sensitivity to the enzyme greatly increased after N-acetylation. The murein was found to contain 30 to 35% glucosamine residues lacking N-acetyl groups. It also contained phosphomuramic acid. Further modifications included amidation of diaminopimelic acid in the peptide side chains and a low alanine content. None of these modifications were found in the murein of another sulphur bacterium, Thiobacillus versutus.
authors.affiliation: Institute of Microbiology, University of Warsaw.
authors.forename: J
authors.initials: J
authors.lastname: Baj
authors.forename: I
authors.initials: I
authors.lastname: Grabowska
authors.forename: Z
authors.initials: Z
authors.lastname: Markiewicz
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D006595:Hexosamines / Q000032:analysis; D066298:In Vitro Techniques; D009112:Muramic Acids / Q000032:analysis; D009113:Muramidase / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry* / Q000302:isolation & purification; D013855:Thiobacillus / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D000606:Amino Sugars; D006595:Hexosamines; D009112:Muramic Acids; D010457:Peptidoglycan; D009113:Muramidase