title: A one-step enzyme immunoassay for the determination of total tissue-type plasminogen activator (t-PA) antigen in plasma.
abstract: A reproducible and sensitive one-step enzyme immunoassay (EIA) was developed to determine total tissue-type plasminogen activator (t-PA) antigen in plasma. The EIA comprises two monoclonal catching antibodies and a polyclonal (goat) tagging antibody conjugated with horseradish peroxidase. There is an equal reactivity towards the several physiological t-PA forms, i.e., single-chain t-PA, two-chain t-PA and t-PA in complex with its naturally occurring inhibitor plasminogen activator inhibitor-type 1 (t-PA/PAI-1 complex). Additionally, the EIA does not discriminate between human melanoma t-PA and recombinant t-PA (Activase). The assay has a lower detection limit of approximately 0.5 ng t-PA per ml plasma, with a time-to-result of only 3.5 h.
authors.affiliation: IVVO-TNO Gaubius Laboratory, Leiden, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Bos
authors.forename: E
authors.initials: E
authors.lastname: Hoegee-de Nobel
authors.forename: R
authors.initials: R
authors.lastname: Laterveer
authors.forename: P
authors.initials: P
authors.lastname: Meyer
authors.forename: W
authors.initials: W
authors.lastname: Nieuwenhuizen
mesh_terms: D000906:Antibodies; D000911:Antibodies, Monoclonal / Q000276:immunology; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D010949:Plasma / Q000737:chemistry; D015849:Plasminogen Inactivators; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D010959:Tissue Plasminogen Activator / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D015849:Plasminogen Inactivators; D011994:Recombinant Proteins; D006735:Horseradish Peroxidase; D010959:Tissue Plasminogen Activator