[Molecular biological study on the overexpression of c-myc gene in uterine cervical carcinomas].
The expression of mRNA for c-myc gene was investigated in 30 cases of human uterine cervical carcinoma by Northern blot hybridization, and c-myc gene amplification was also examined by Slot blot hybridization. Overexpression of c-myc gene was detected in one (7.7%) of 13 cases of carcinoma in situ (stage 0), one (10.0%) of 10 stage I and 4 (57.1%) of 7 stage II uterine carcinoma, and c-myc gene amplification was detected in 2 cases (6.7%) in which c-myc gene overexpression was also found. The patient whose c-myc mRNA level was more than 10 times as high as in normal uterine cervical tissue relapsed and died within 2 years after the first treatment. Our results suggest that the overexpression of c-myc gene occurred more frequently in advanced than in early uterine cervical carcinoma and that it might be useful in prognosing cervical cancer.
Tanimoto
H
H
Department of Obstetrics and Gynecology, Hiroshima University School of Medicine.
Nagai
N
N
Fujimoto
H
H
Ohta
S
S
Ohama
K
K
D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D002278:Carcinoma in Situ / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis*; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D012333:RNA, Messenger