title: Protein-tyrosine kinase p72syk is activated by wheat germ agglutinin in platelets.
abstract: We previously reported a molecular cloning of porcine gene syk encoding a non-receptor type 72-kDa protein-tyrosine kinase (Taniguchi et al. (1991) J. Biol. Chem. 266, 15790-15796). In this study, we have demonstrated that p72syk is expressed in porcine platelets at 0.1-0.2% of total protein and that the lectin wheat germ agglutinin induces an activation of p72syk against both auto- and exogenous-substrate-phosphorylation in porcine platelets. The activation of p72syk was abrogated by the coexistence of N-acetyl-D-glucosamine with wheat germ agglutinin. These data suggest that p72syk is a candidate of responsible protein-tyrosine kinase for platelet activation and that cell surface glycoprotein is involved in the activation of p72syk in platelets.
authors.affiliation: Department of Biochemistry, Fukui Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ohta
authors.forename: T
authors.initials: T
authors.lastname: Taniguchi
authors.forename: M
authors.initials: M
authors.lastname: Asahi
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: G
authors.initials: G
authors.lastname: Nakagawara
authors.forename: H
authors.initials: H
authors.lastname: Yamamura
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology; D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D001792:Blood Platelets / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D015151:Immunoblotting; D008214:Lymphocytes / Q000201:enzymology; D008970:Molecular Weight; D010750:Phosphoproteins / Q000097:blood; D011505:Protein-Tyrosine Kinases / Q000097:blood*; D013347:Subcellular Fractions / Q000201:enzymology; D013552:Swine; D014909:Wheat Germ Agglutinins
chemical_list: D000596:Amino Acids; D010750:Phosphoproteins; D014909:Wheat Germ Agglutinins; D011505:Protein-Tyrosine Kinases; D000117:Acetylglucosamine