title: The role of the visceral yolk sac in hyperglycemia-induced embryopathies in mouse embryos in vitro.
abstract: The adverse developmental effects of hyperglycemia to rodent embryos have been shown using whole embryo culture. Although, a mechanism by which hyperglycemia-induced effects occur is unknown, recent work has focused on the visceral yolk sac as a potential target tissue. Therefore, we have evaluated the developmental effects of hyperglycemia in early head fold stage mouse embryos in vitro and assessed the histiotrophic function of the visceral yolk sac. As has been previously shown in rodents, hyperglycemia produced neural tube closure defects in a concentration dependent manner at 33, 50, and 67 mM glucose using a 44 h exposure period. However, exposure times between 6 and 12 h were sufficient to alter embryonic development when the glucose concentration was 50 or 67 mM. In contrast, early somite stage embryos (4-6 somite stage) appear to be less sensitive to dysmorphogenesis and a 48 h exposure to 67 mM glucose but not 33 or 50 mM also produced neural tube defects. Hyperglycemia (67 mM) did not alter the uptake of 35S-methionine and 35S-cysteine-labeled hemoglobin (35S-Hb) in the visceral yolk sac (VYS) in early headfold staged embryos. However, the accumulation of 35S in the embryo was reduced by 16-18% at glucose concentrations of 50 or 67 mM during the last 12 h of a 44 h exposure period. No effect on VYS uptake or embryonic accumulation of 35S-labeled products was observed at shorter exposure periods (12-24 and 24-36 h).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Cell Biology and Anatomy, School of Medicine, University of North Carolina, Chapel Hill 27599.
authors.forename: E S
authors.initials: ES
authors.lastname: Hunter
authors.forename: T W
authors.initials: TW
authors.lastname: Sadler
mesh_terms: D000818:Animals; D000013:Congenital Abnormalities / Q000209:etiology; D046508:Culture Techniques; D005260:Female; D005315:Fetal Diseases / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D005947:Glucose / Q000009:adverse effects; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006943:Hyperglycemia / Q000150:complications*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010873:Pinocytosis / Q000502:physiology; D011247:Pregnancy; D013462:Sulfur Radioisotopes; D015017:Yolk Sac / Q000502:physiology*
chemical_list: D006454:Hemoglobins; D013462:Sulfur Radioisotopes; D005947:Glucose