Influence of oxygen radicals generating agents on eicosanoid metabolism of human endothelial cells.
Human umbilical venous endothelial cells were incubated with increasing concentrations (0.001-10 mmol/l) of H202 or cumene hydroperoxide. PGI2 was released within the first five minutes, whereas a significant increase in TXA2-release was not observed before 10 minutes after stimulation. Furthermore the content of intracellular ATP and the level of cytosolic free Ca2+ were measured. Doses of 0.01 mmol/l H202 or cumene hydroperoxide increased the intracellular ATP-level. Doses higher than 0.1 mmol/l led to a decrease of ATP. The cytosolic free calcium (Ca2+) increased on stimulating the cells with 0.1 mmol/l cumene hydroperoxide between 120 and 240 seconds after stimulation, whereas 0.1 mmol/l H202 had no effects. These data give strong evidence, that eicosanoid-synthesis is directly started by oxygen radicals, followed by an elevation of intracellular free Ca2+.
Griesmacher
A
A
2nd Department of Surgery/Division of Clinical Chemistry, University of Vienna, Austria.
Weigel
G
G
David
M
M
Schimke
I
I
Mueller
M M
MM
D000255:Adenosine Triphosphate / Q000378:metabolism; D001555:Benzene Derivatives / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis* / Q000378:metabolism; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D010100:Oxygen / Q000502:physiology*; D013928:Thromboxane A2 / Q000096:biosynthesis* / Q000378:metabolism
D001555:Benzene Derivatives; D005609:Free Radicals; D013928:Thromboxane A2; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide; D011464:Epoprostenol; C007164:cumene hydroperoxide; D010100:Oxygen; D002118:Calcium