title: K-current mediation of prolactin-induced proliferation of malignant (Nb2) lymphocytes.
abstract: The effects of different concentrations of various K-current blockers on prolactin-induced proliferation and membrane K-currents in malignant lymphocytes (Nb2 cells) were investigated. Membrane currents were measured with the whole cell patch-clamp technique, and lymphocyte density was quantified by both spectrophotometric and conventional methods. K-current blockers tested (quinidine, 4-aminopyridine, barium, and tetraethylammonium) exhibited similar rank order potency for K-current block and inhibition of prolactin-induced proliferation of malignant lymphocytes. Because Nb2 cells proliferate independently of a transmembrane Ca-influx, these results suggest that K-currents per se rather than K-current modulation of Ca-influx is an essential event for lymphocyte proliferation.
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside 92521-0121.
authors.forename: Y F
authors.initials: YF
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Jia
authors.forename: A M
authors.initials: AM
authors.lastname: Walker
authors.forename: S
authors.initials: S
authors.lastname: Cukierman
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D008214:Lymphocytes / Q000473:pathology* / Q000502:physiology; D008223:Lymphoma / Q000473:pathology / Q000503:physiopathology; D015221:Potassium Channels / Q000378:metabolism / Q000502:physiology*; D011388:Prolactin / Q000494:pharmacology*; D011802:Quinidine / Q000494:pharmacology; D051381:Rats; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000473:pathology
chemical_list: D015221:Potassium Channels; D013757:Tetraethylammonium Compounds; D001464:Barium; D019789:Tetraethylammonium; D011388:Prolactin; D015761:4-Aminopyridine; D011802:Quinidine