In vitro multiplication of Cryptosporidium parvum in mouse peritoneal macrophages.
Cryptosporidium parvum of bovine origin was developed in vitro in unsensitized mouse peritoneal macrophages. Macrophages growing in RPMI medium were infected with sporozoites or with oocysts, and after staining infections were studied by light microscopy. A high parasitic index was obtained with multiple infections occurring commonly. This is a simple method for the study of Cryptosporidium biology, and for in vitro assays of pharmacological activity.
Martinez
F
F
Department of Molecular Parasitology, School of Science, University of Granada, Spain.
Mascaro
C
C
Rosales
M J
MJ
Diaz
J
J
Cifuentes
J
J
Osuna
A
A
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D002478:Cells, Cultured; D003457:Cryptosporidiosis / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000254:growth & development*; D005243:Feces / Q000469:parasitology; D008264:Macrophages / Q000469:parasitology*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology