title: Intestinal blood flow is restored with glutamine or glucose suffusion after hemorrhage.
abstract: Intestinal blood flow has been shown to be impaired after resuscitated hemorrhagic shock. Enteral feeding has been proposed as an adjunct for preserving mucosal integrity and decreasing translocation-related morbidities during stress. The purpose of this study was to determine if an ileal mucosal suffusion with an isotonic glucose or glutamine solution begun after resuscitation would prevent development of this blood flow impairment. The distal ileum of anesthetized Sprague-Dawley rats was prepared for in vivo videomicroscopy. Animals were bled to 50% of baseline blood pressure for 60 min and then resuscitated with their shed blood and an equal volume of lactated Ringer's. After resuscitation was complete, the mucosa was suffused with isotonic glucose, glutamine, or saline (control). Resuscitation restored cardiac output and mean arterial pressure to baseline in all groups; however, first-order arteriolar blood flow remained 50% below baseline in the saline group. Glucose-treated animals demonstrated a 34% increase over baseline in first-order arteriolar blood flow 120 min after resuscitation due to submucosal and previllus arteriolar dilation. This effect became evident 30 min after initiating the suffusion, suggesting an effect mediated via locally generated vasodilators. Glutamine suffusion attenuated the flow impairment by dilation of previllus arterioles but to a lesser degree than that observed in glucose-treated animals. These data demonstrate that mucosal suffusion with an isotonic glucose solution overrides the residual effects of hemorrhagic shock on the intestinal microcirculation and suggest a mechanism for preserving mucosal integrity with the addition of glutamine to standard enteral formulations.
authors.affiliation: Department of Surgery, University of Louisville School of Medicine, Kentucky 40292.
authors.forename: W J
authors.initials: WJ
authors.lastname: Flynn
authors.forename: J R
authors.initials: JR
authors.lastname: Gosche
authors.forename: R N
authors.initials: RN
authors.lastname: Garrison
mesh_terms: D000818:Animals; D001160:Arterioles / Q000503:physiopathology; D005947:Glucose / Q000502:physiology*; D005973:Glutamine / Q000494:pharmacology*; D006470:Hemorrhage / Q000503:physiopathology* / Q000628:therapy; D007422:Intestines / Q000098:blood supply*; D007552:Isotonic Solutions; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow / Q000187:drug effects; D012151:Resuscitation; D014661:Vasoconstriction
chemical_list: D007552:Isotonic Solutions; D005973:Glutamine; D005947:Glucose