Effects of epidermal growth factor on apo B mRNA levels and apo B accumulation in the media of primate hepatocytes in culture.
EGF has been shown to augment albumin and apolipoprotein A-I secretion by cynomolgus monkey hepatocytes in primary culture without stimulating cell division. This study was undertaken to determine what effect EGF had on apo B secretion by those hepatocytes. The results indicate that EGF (3 nM final concentration) severely inhibits the rate at which apo B accumulates in the culture medium of primate hepatocytes. That effect was evident within 48 hours of treatment, and by 72 hours the rate that apo B accumulated was less than half that of cells treated with a hormone-free medium. However, the apo B mRNA levels in the EGF-treated cells were more than double those of hepatocytes given the hormone-free medium. These data indicate that EGF has a potent effect on the rate at which apo B accumulates in the culture medium of primate hepatocytes and that the effect is independent of apo B gene expression.
Blake
W L
WL
Upjohn Laboratories, Kalamazoo, MI 49001.
Vidmar
T J
TJ
Melchior
G W
GW
D000704:Analysis of Variance; D000818:Animals; D016632:Apolipoprotein A-I / Q000378:metabolism; D001055:Apolipoproteins B / Q000235:genetics* / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008252:Macaca fascicularis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism; D013997:Time Factors
D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D012333:RNA, Messenger; D012709:Serum Albumin; D004815:Epidermal Growth Factor