Fibroblast cultures of patients with basal cell epithelioma exhibit a normal sensitivity to the genotoxic effect of ultraviolet irradiation.
Fibroblast cultures of 16 basal cell epithelioma (basalioma, BCE) patients with an unusually young age at onset of disease (29-51 years; 42.5 +/- 7.04), and healthy normal controls (27-55 years; 40.73 +/- 9.52) were studied for chromosome instability induced by ultraviolet rays (UV). We used an UV source that emitted predominantly UV-A and UV-B at an intensity of 375 J/m2 and evaluated the induction of micronuclei (MN) and sister chromatid exchange (SCE). Young basalioma patients and normal controls showed no significant differences in MN and SCE frequencies, neither with respect to spontaneous nor to UV-induced values (MN spontaneous: 10.80 +/- 5.65 vs. 11.32 +/- 8.21; UV-induced increase: 7.36 +/- 4.40 vs. 9.93 +/- 7.55; SCE spontaneous: 10.28 +/- 1.61 vs. 10.72 +/- 1.09; UV-induced increase: 7.30 +/- 2.19 vs. 7.55 +/- 2.14). We conclude from these data that an enhanced UV sensitivity as observed in cells from patients with cutaneous malignant melanoma and xeroderma pigmentosum is not a constitutive risk factor in basalioma patients.
Rümmelein
B
B
Unit of Toxicogenetics, University of Hamburg, Germany.
Eloo
U
U
Bielfeld
V
V
Ehlert
U
U
Weichenthal
M
M
Breitbart
E W
EW
Rüdiger
H W
HW
D000328:Adult; D002280:Basal Cell Carcinoma / Q000235:genetics*; D004247:DNA / Q000528:radiation effects*; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects; D008875:Middle Aged; D012854:Sister Chromatid Exchange / Q000528:radiation effects; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays / Q000009:adverse effects*
D004247:DNA