title: Effects of estradiol treatment on responses of rat preoptic warm sensitive neurons to progesterone in vitro.
abstract: Single unit activity was recorded extracellularly in preoptic slice preparations from ovariectomized female rats. The rats were pretreated daily with (1) estradiol benzoate (20 micrograms/0.1 ml sesame oil) for 3 consecutive days just prior to the experiment (OVX+E72h), (2) sesame oil (OVX+Oil), or (3) untreated (OVX). In the preparations from OVX rats, 12 (50%) of 24 warm-sensitive neurons were inhibited and 2 (8%) were excited by the application of progesterone (P) in perfusion medium (30 ng/ml). In OVX+Oil preparations, 4 (40%) of 10 warm-sensitive neurons were inhibited and none was excited by P. In OVX+E72h preparations, however, only 4 (10%) were inhibited by P and 16 (38%) of 42 warm-sensitive neurons were excited. When Ru38486, a P-receptor antagonist, was applied together with P, no excitation was observed in warm-sensitive neurons in OVX+E72h preparations. These findings suggest that the P-induced excitation of preoptic warm sensitive neurons is mediated through the estrogen-induced P-receptors.
authors.affiliation: Department of Marine Resources, National Sun Yat-sun University, Kaohsiung, Taiwan, ROC.
authors.forename: C L
authors.initials: CL
authors.lastname: Tsai
authors.forename: K
authors.initials: K
authors.lastname: Kanosue
authors.forename: K
authors.initials: K
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D001833:Body Temperature Regulation / Q000187:drug effects / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology*; D004971:Estrus / Q000502:physiology; D005260:Female; D015735:Mifepristone / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D010052:Ovariectomy; D011301:Preoptic Area / Q000187:drug effects*; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011980:Receptors, Progesterone / Q000037:antagonists & inhibitors / Q000187:drug effects; D012715:Sesame Oil / Q000494:pharmacology
chemical_list: D011980:Receptors, Progesterone; D015735:Mifepristone; D011374:Progesterone; D004958:Estradiol; D012715:Sesame Oil