Immunoreactivities for antisera to three putative neuropeptides of the rat melanin-concentrating hormone precursor are coexpressed in neurons of the rat lateral dorsal hypothalamus.
Antisera (AS) raised against rat melanin-concentrating hormone (rMCH) and against two additional peptides sequences derived from the rat MCH precursor (neuropeptide glutamic acid-isoleucineamide (NEI), and neuropeptide glycine-glutamic acid (NGE)) exclusively stained the hypothalamic neurons previously described using AS to salmon MCH, human somatocrinin 1-37 (GRF37) and alpha-MSH. Liquid phase and dot-blot controls for specificity indicated that rMCH-, NEI- and NGE-AS bound epitopes recognized by sMCH-, alpha-MSH- and GRF-37-AS, respectively. The distinct intracellular patterns of immunoreactivity obtained in control animals with rMCH-, NGE- and NEI-AS, as well as the changes observed after intracerebroventricular injection of colchicine matched previous findings using sMCH-, GRF37- and alpha-MSH-AS.
Risold
P Y
PY
CNRS URA 0561, Laboratoire d'Histologie Embryologie, Cytogénétique, Faculté de Médecine, Besançon, France.
Fellmann
D
D
Rivier
J
J
Vale
W
W
Bugnon
C
C
D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D007028:Hypothalamic Hormones / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D015151:Immunoblotting; D008297:Male; D008543:Melanins / Q000096:biosynthesis / Q000378:metabolism*; D009474:Neurons / Q000276:immunology / Q000378:metabolism*; D009479:Neuropeptides / Q000276:immunology / Q000378:metabolism*; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism*; D010907:Pituitary Hormones / Q000096:biosynthesis / Q000378:metabolism*; D011498:Protein Precursors; D051381:Rats; D011919:Rats, Inbred Strains
D007028:Hypothalamic Hormones; D008543:Melanins; D009479:Neuropeptides; D010446:Peptide Fragments; D010907:Pituitary Hormones; D011498:Protein Precursors; C075863:melanin concentrating hormone precursor (109-129)-glycyl-glutamic acid; C075862:melanin-concentrating-hormone precursor (129-145)-glutamyl-isoleucinamide; C059991:melanin-concentrating hormone precursors; C019463:melanin-concentrating hormone; D003078:Colchicine