title: [A simple method of isolating monoclonal immunoglobulin M, possessing rheumatoid activity].
abstract: A simple procedure for obtaining highly purified preparations of native monoclonal (Waldenström's disease) immunoglobulin M possessing a rheumatoid activity (IgM-RF) has been developed. The method is based on the use of affinity chromatography with a new readily available adsorbent (immunoglobulin G-porous glass) and 3 M LiCl in Tris-buffer pH 8.3-8.4 able to induce the dissociation of the IgM-RF-IgG complex. The IgM-RF preparation thus obtained was characterized in terms of amino acid composition (relative to conventional monoclonal IgM), carbohydrate composition and structure of oligosaccharide moieties of a complex type. It was shown that some dissociation conditions for the IgM-RF-IgG complex routinely used to isolate IgM-RF provoke irreversible denaturation of IgM-RF when applied to a preliminarily purified complex.
authors.forename: V A
authors.initials: VA
authors.lastname: Lapuk
authors.forename: A I
authors.initials: AI
authors.lastname: Chukhrova
authors.forename: E V
authors.initials: EV
authors.lastname: Chernokhvostova
authors.forename: V A
authors.initials: VA
authors.lastname: Aleshkin
authors.forename: G P
authors.initials: GP
authors.lastname: German
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Varlamova
authors.forename: A M
authors.initials: AM
authors.lastname: Ponomareva
authors.forename: N P
authors.initials: NP
authors.lastname: Arbatskiĭ
authors.forename: A O
authors.initials: AO
authors.lastname: Zheltova
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000276:immunology*; D008969:Molecular Sequence Data; D011489:Protein Denaturation; D012217:Rheumatoid Factor / Q000276:immunology*
chemical_list: D000596:Amino Acids; D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D007075:Immunoglobulin M; D012217:Rheumatoid Factor
vernacular_title: Prostoĭ metod vydeleniia monoklonal'nogo immunobloulina M, obladaiushchego revmatoidnoĭ aktivnost'iu.