Amino acid sequence of the N-terminal domain of yam (Dioscorea japonica) aerial tuber acidic chitinase. Evidence for the presence of a wheat germ agglutinin domain in matured acidic chitinase from unstressed tuber.
The amino acid sequence of the N-terminal domain of acidic chitinase from unstressed aerial tuber was determined and proved the presence of an N-terminal domain in acidic chitinase. The amino acid sequence was determined on a pyroglutamylaminopeptidase-treated N-terminal fragment of V8 protease and on chymotryptic peptides of this fragment. The sequence determined revealed 8 residues deletion and 2 residues insertion as compared with the N-terminal domain of tobacco basic chitinase. The N-terminal domain determined showed a homology of 40% and 52% with the N-terminal domain of tobacco basic chitinase and wheat germ agglutinin, respectively.
Araki
T
T
Department of Biochemistry, School of Agriculture, Kyushu Tokai University, Kumamoto, Japan.
Funatsu
J
J
Kuramoto
M
M
Torikata
T
T
D000595:Amino Acid Sequence; D002688:Chitinases / Q000737:chemistry* / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D037121:Plant Lectins; D010944:Plants / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases; D014909:Wheat Germ Agglutinins / Q000737:chemistry*
D010446:Peptide Fragments; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins; D002688:Chitinases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase