Gene expression of metalloproteinase and its inhibitor in mesangial cells exposed to high glucose.
To clarify the roles of metalloproteinases and their inhibitor (TIMP) in diabetic glomerulopathy, we studied the effect of a high glucose concentration on the gene expression of metalloproteinase transin and TIMP as well as collagen type IV and laminin in cultured rat mesangial cells (MCs). In the high glucose group, collagen type IV, laminin, and TIMP mRNA levels were all elevated in a concentration-dependent manner, whereas transin expression was suppressed. Osmotic control of high glucose with mannitol selectively stimulated TIMP expression. We hypothesize that high glucose decreases matrix-degrading activity as well as increases matrix productivity in MCs.
Kitamura
M
M
Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.
Kitamura
A
A
Mitarai
T
T
Maruyama
N
N
Nagasawa
R
R
Kawamura
T
T
Yoshida
H
H
Takahashi
T
T
Sakai
O
O
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects; D005920:Glomerular Mesangium / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D006023:Glycoproteins / Q000235:genetics*; D007375:Interleukin-1 / Q000494:pharmacology; D007700:Kinetics; D007797:Laminin / Q000235:genetics; D008297:Male; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000235:genetics*; D011347:Procollagen / Q000235:genetics; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors; D019714:Tissue Inhibitor of Metalloproteinases
D006023:Glycoproteins; D007375:Interleukin-1; D007797:Laminin; D011347:Procollagen; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019714:Tissue Inhibitor of Metalloproteinases; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D005947:Glucose