Stimulatory effect of interleukin-1 beta on the interferon-gamma-dependent HLA-DR production.
Expression of major histocompatibility complex (MHC) class II genes is induced by interferon-gamma (IFN-gamma) in antigen-presenting cells. When human leukaemia THP-1 and PL-21 cells were pretreated with phorbol ester, IFN-gamma-dependent induction of MHC class II gene was markedly enhanced. To elucidate the mechanism of the phenomenon, we examined the effect of cytokines which were secreted from THP-1 cells by the treatment of phorbol ester. Among those cytokines, interleukin-1 beta (IL-1 beta) had a positive effect on IFN-gamma-dependent induction of MHC class II genes. In order to rule out the possibility that different clones of THP-1 cells respond independently to IFN-gamma or phorbol ester, we showed that all independent single clones expressed MHC class II and produced IL-1 beta by IFN-gamma or phorbol ester, respectively.
Tomoda
T
T
Department of Pediatrics, Kochi Medical School, Japan.
Kurashige
T
T
Taniguchi
T
T
D003470:Culture Media; D016207:Cytokines / Q000096:biosynthesis; D015793:HLA-B7 Antigen / Q000235:genetics; D006684:HLA-DR Antigens / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D007371:Interferon-gamma / Q000276:immunology*; D007375:Interleukin-1 / Q000276:immunology*; D007951:Leukemia, Myeloid / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D016207:Cytokines; D015793:HLA-B7 Antigen; D006684:HLA-DR Antigens; D007375:Interleukin-1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate