Diagnosis of induced cleft palate in rats from defective patterns of differentiation of isoenzymes.
Cleft palate was induced in 420 embryos of Sprague-Dawley rats with a single oral dose of 600 mg/kg beta-aminoproprionitrile (BAPN) on embryonal day 15, 7 hours. The cleft palate was accompanied by a pathological differentiation pattern of various isoenzymes in palatal shelves. These isoenzymes could be detected in amniotic fluid from the 16th to the 20th days of pregnancy when they also had a pathological differentiation pattern. We conclude that teratogenically induced cleft palate in rats is accompanied by a pathological differentiation pattern that can be traced by determination of isoenzymes in the palatal shelves as well as in amniotic fluid.
Granström
G
G
Department of Oto-Rhino-Laryngology, University of Gothenburg, Sweden.
Jacobsson
C
C
Kirkeby
S
S
D000014:Abnormalities, Drug-Induced / Q000175:diagnosis* / Q000473:pathology; D000629:Aminopropionitrile / Q000031:analogs & derivatives; D000653:Amniotic Fluid / Q000201:enzymology*; D000818:Animals; D002972:Cleft Palate / Q000139:chemically induced / Q000175:diagnosis* / Q000473:pathology; D004796:Clinical Enzyme Tests; D005260:Female; D007527:Isoenzymes / Q000032:analysis*; D010159:Palate / Q000196:embryology / Q000201:enzymology* / Q000473:pathology; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; C027243:beta-aminopropionitrile fumarate; D000629:Aminopropionitrile