Deoxyribonucleic acid (DNA) typing of human leukocyte antigen (HLA)-DQA1 from single hairs in Japanese.
The deoxyribonucleic acid (DNA) typing of human leukocyte antigen (HLA)-DQA1 from single hairs is described. HLA-DQA1 genotypes could be determined from single plucked hair roots. However, it was not easy to type HLA-DQA1 with hair shaft portions. Increase in the specimens of hair shaft portions (over 10 cm in length) to get sufficient DNA caused inhibition of polymerase chain reaction (PCR). Synthetic melanin as well as the one extracted from hairs inhibited the PCR of the genomic DNA template when added to the PCR reaction at the concentrations over than 15 ng/100 microL. Therefore, typability of hair shaft portions seems to depend on the delicate balance of the concentrations of DNA and the contaminated melanin in the final DNA extracts.
Uchihi
R
R
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
Tamaki
K
K
Kojima
T
T
Yamamoto
T
T
Katsumata
Y
Y
D044466:Asian People / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D005819:Genetic Markers; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D006197:Hair / Q000737:chemistry*; D006579:Heterozygote; D006801:Humans; D007564:Japan; D008543:Melanins / Q000032:analysis; D016133:Polymerase Chain Reaction
D005819:Genetic Markers; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D008543:Melanins; D004247:DNA