Study on fatty acid binding by proteins in yeast. Dissimilar results in Saccharomyces cerevisiae and Yarrowia lipolytica.
1. The presence of soluble proteins with fatty acid binding activity was investigated in cell-free extracts from Saccharomyces cerevisiae and Yarrowia lipolytica cultures. 2. No significant fatty acid binding by proteins was detected in S. cerevisiae, even when grown on a fatty acid-rich medium, thus indicating that such proteins are not essential to fatty acid metabolism. 3. An inducible fatty acid binding protein (K0.5 = 3-4 microM) was found in Y. lipolytica which had grown on a minimal medium with palmitate as the sole source of carbon and energy. 4. The relative molecular mass of this protein was 100,000 as inferred from Sephacryl S-200 gel filtration.
Dell'Angelica
E C
EC
Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
Stella
C A
CA
Ermácora
M R
MR
Ramos
E H
EH
Santome
J A
JA
D002352:Carrier Proteins / Q000378:metabolism*; D002850:Chromatography, Gel; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D007700:Kinetics; D009363:Neoplasm Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*
D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005656:Fungal Proteins; D009363:Neoplasm Proteins