An ELISA utilizing immobilised snowdrop lectin GNA for the detection of envelope glycoproteins of HIV and SIV.
A simple, reliable ELISA for the quantitative detection of the envelope glycoproteins of both HIV and SIV is described. It incorporates the snowdrop lectin GNA to capture the glycoprotein antigens and combines the high selectivity of GNA binding with its broad reactivity with the glycoproteins of HIV-1, HIV-2 and SIV.
Mahmood
N
N
MRC Collaborative Centre, Mill Hill, London, UK.
Hay
A J
AJ
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015497:HIV-1 / Q000737:chemistry*; D015498:HIV-2 / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000737:chemistry*; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; D015302:Simian Immunodeficiency Virus / Q000737:chemistry*; D014759:Viral Envelope Proteins / Q000032:analysis*
D037102:Lectins; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; D014759:Viral Envelope Proteins; C063117:snowdrop lectin