[Production and characterization of a group of monoclonal antibodies to human transitional cell carcinoma].
A panel of specific mouse monoclonal antibodies, BIU-H4, BIU-H6, BIU-H9, was obtained by immunization with BIU-87 cells (a transitional cell carcinoma cell line of the bladder), which was blocked by the serum of mouse anti-human lymphocyte. Detailed descriptions were given concerning their reactivity patterns by ABC-ELISA and IIF assays against various cells. They showed positive reactions with transitional cell carcinoma of the bladder and negative with normal bladder. BIU-H6 did not react with any of the other normal or malignant tissues. The subclass of immunoglobulin for BIU-H4,6 was IgG1, for BIU-H9 is IgG2 alpha. The molecular weight of the antigen recognized by BIU-H6,9 is 102 KD.
Guo
Y L
YL
Institute of Urology, Beijing Medical University.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000918:Antibody Specificity; D002295:Carcinoma, Transitional Cell / Q000276:immunology* / Q000473:pathology; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal