New equations and a method for finding nine parameter values for two alleles at one locus to study gene conversion using Ascobolus immersus.
A quantitative treatment is given for meiotic gene conversion with its parameters and equations for their interactions to determine allele segregation class frequencies from heterozygotes. The possible pairing of both pairs of nonsister chromatids in a bivalent at exactly the same point is included. Using sets of data from Ascobolus immersus, it is shown that values for all nine parameters for hybrid DNA models of recombination can be obtained using an iterative computer program. The accuracy of the values is estimated and the double-strand gap repair model is considered. The parameter values obtained invalidate most of the simplifications used in previous quantitative analyses of gene conversion data. They showed total bias in strand preference in asymmetric hybrid DNA formation and some bias in which type of chromatid is the invading one. There were slight differences in repair frequency between the two types of mispair and very large differences in the direction of repair. Conversion control factors had major effects on hybrid DNA formation and repair of mispairs.
Lamb
B C
BC
Department of Biology, Imperial College, London, U.K.
Zwolinski
S A
SA
D000483:Alleles; D001203:Ascomycota / Q000235:genetics*; D002874:Chromosome Mapping; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics; D005785:Gene Conversion; D006579:Heterozygote; D008433:Mathematics; D008957:Models, Genetic; D011995:Recombination, Genetic
D004271:DNA, Fungal