Inhibition of human immunodeficiency virus-1 infection by human conglutinin-like protein: in vitro studies.
The lectin-like protein analogous to bovine conglutinin was purified from human serum. The carbohydrate-binding ability of conglutinin-like protein was inhibited by D-mannose, N-acetylglucosamine and L-fucose as well as by mannan-containing oligosaccharides. By applying a lectin-based ELISA system it was demonstrated that conglutinin-like protein binds to human immunodeficiency virus-1 (HIV-1) glycoprotein 120 (gp120) via its carbohydrate binding site. In vitro experiments with T-lymphoblastoid CEM cells revealed that conglutinin-like protein abolishes infection by HIV-1; a 50% cytoprotective concentration of 23.9 micrograms/ml was measured. These findings demonstrate that human conglutinin-like protein binds to HIV-gp120 and inhibits, under the described in vitro conditions, CEM cell infection.
Ushijima
H
H
Division of AIDS Virus, AIDS Research Center, National Institute of Health, Tokyo, Japan.
Schröder
H C
HC
Poznanovic
S
S
Gasić
M J
MJ
Matthes
E
E
Müller
W E
WE
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology / Q000517:prevention & control*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007116:Immunization, Passive; D037561:Immunoconglutinins; D007136:Immunoglobulins / Q000187:drug effects* / Q000378:metabolism; D037601:Mannose-Binding Lectin; D008969:Molecular Sequence Data; D014764:Viral Proteins / Q000378:metabolism
D002241:Carbohydrates; D002352:Carrier Proteins; D015699:HIV Envelope Protein gp120; D037561:Immunoconglutinins; D007136:Immunoglobulins; D037601:Mannose-Binding Lectin; D014764:Viral Proteins; C069858:mannose binding protein A