Tetracycline resistance genes in Kenyan hospital isolates of Salmonella typhimurium.
All 97 strains of Salmonella typhimurium isolated from patients at a hospital in Nairobi, Kenya, during 1988-90 were resistant to tetracycline. The minimum inhibitory concentration (MIC) showed a large distribution range from 1 microgram/ml to 128 micrograms/ml. The strains were heterogeneous with respect to plasmid content, but initially all strains possessed, in addition to other plasmids, a large 60-, 63- or 65-MDa plasmid. The tetracycline resistance genes were characterized using oligonucleotide probes, and 20% of the resistant strains possessed tetracycline type A (tetr A), 6% tetr B, and 4% tetrC genes. Three strains possessed both type A and B tetracycline resistance determinants, which were shown to be located on the large 65-MDa plasmid. There was no correlation between strains isolated from stools, blood, cerebrospinal or epidural fluids, pus, or urine, with respect to the tetracycline genotypes, MIC values or plasmid content.
Kariuki
S
S
Department of Microbiology and Immunology, Norwegian College of Veterinary Medicine, Oslo.
Mirza
N B
NB
Wasteson
Y
Y
Senerwa
D
D
Gathuma
J M
JM
Olsvik
O
O
D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006761:Hospitals; D006801:Humans; D007630:Kenya; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012480:Salmonella Infections / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D013753:Tetracycline Resistance / Q000235:genetics*
D015342:DNA Probes