Determination of panaxadiol and panaxatriol in ginseng and its preparations by capillary supercritical fluid chromatography (SFC).
Capillary supercritical fluid chromatographic (SFC) method has been developed for the determination of panaxadiol and panaxatriol in ginseng and its preparations. 0.1 g ginseng or an appropriate amount of its preparations was hydrolysed by 15% H2SO4 in an ethanol:water (1:1 v/v) solution for 4 h followed by 15% NaOH for 0.5 h. The mixture was extracted by cyclohexane. The cyclohexane extracts were purified by a partition column and concentrated by an adsorption column and then analysed by SFC. Methyltestosterone was used as the internal standard.
Li
Y H
YH
College of Pharmacy, Second Military Medical University, Shanghai, P.R. China.
Li
X L
XL
Hong
L
L
Liu
J Y
JY
Zhang
M Y
MY
D002845:Chromatography / Q000379:methods*; D005410:Flame Ionization; D036145:Ginsenosides; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal; D012503:Saponins / Q000032:analysis*; D014315:Triterpenes / Q000032:analysis*
D036145:Ginsenosides; D012503:Saponins; D014315:Triterpenes; C004564:panaxadiol; C004563:panaxatriol