title: Expression of calbindin-D28k in developing and growing chick testes.
abstract: Calbindin, a 28-kDa vitamin D-dependent calcium-binding protein was localized immunohistochemically in developing and growing chick testes. The protein first appeared in the germinal epithelium of developing testes of the eight-day-old embryo and remained therein throughout development. Calbindin was not present in the germinal epithelium after hatching. Calbindin was next detected in the spermatogonia and spermatocytes of one-week-old and growing chick testes. Calbindin-positive spermatogonia and spermatocytes gradually increased in number and staining intensity as the seminiferous tubules further developed. A few interstitial Leydig cells were positive for calbindin from five-week-old and older chicks. Comparison of the time-course of appearance and increase in calbindin content in spermatogonia and spermatocytes with spermatogenesis in chickens suggests that calbindin may be involved in the mitotic process in spermatogonia and spermatocytes.
authors.affiliation: Department of Veterinary Anatomy and Cellular Biology OSU, Columbus, OH 43210.
authors.forename: N
authors.initials: N
authors.lastname: Inpanbutr
authors.forename: A N
authors.initials: AN
authors.lastname: Taylor
mesh_terms: D000818:Animals; D064026:Calbindins; D002642:Chick Embryo; D002645:Chickens; D007150:Immunohistochemistry; D007985:Leydig Cells / Q000737:chemistry*; D008297:Male; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D013090:Spermatocytes / Q000737:chemistry*; D013093:Spermatogonia / Q000737:chemistry*; D013737:Testis / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development
chemical_list: D064026:Calbindins; D064030:S100 Calcium Binding Protein G