Large-scale molecular mapping of human c-myb locus: c-myb proto-oncogene is not involved in 6q- abnormalities of lymphoid tumors.
Chromosomal abnormalities in the 6q region have been observed frequently in several T-cell and myeloid leukemias. Interestingly, this region was found to contain three cellular oncogenes, c-myb, c-fyn and c-ros. Several of the tumors that exhibit 6q- abnormalities have also been found to express high levels of c-myb and, in some cases, amplification of the c-myb gene, leading to the suggestion that this gene could lie in proximity to the deletions observed in these tumors. To determine if c-myb gene activation is associated with 6q- abnormalities, we developed a megabase map of the human c-myb locus using pulsed-field gel electrophoresis. We then examined the occurrence of abnormalities near the c-myb gene in several hematopoietic tumor cell lines containing well-characterized 6q- abnormalities. Our results show that no rearrangements or deletions occur within a region of 1.0 Mbp of the c-myb locus in these cell lines. However, several of the cell lines exhibited differential and partial methylation patterns which seem to be prevalent amongst different cell lines.
Park
J G
JG
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Reddy
E P
EP
D015139:Blotting, Southern; D002869:Chromosome Aberrations; D002896:Chromosomes, Human, Pair 6; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D008745:Methylation; D009857:Oncogenes / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured