Testosterone-induced diminution of two peptides in spleen cells from testosterone-immunosuppressed B10 mice.
High-performance liquid chromatography (HPLC) is used to detect testosterone (T)-sensitive peptides in spleen cells isolated from female C57BL/10 mice immunosuppressed against Plasmodium chabaudi malaria by T treatment. Two peaks with retention times of about 25 min and 34 min, respectively, were identified to be diminished by about 52% and 47%, respectively, in spleen cells from T-treated mice compared to those from untreated mice. Amino acid sequencing revealed that the 24 min peak consisted of the dipeptide Met-Phe and the 34 min peak contained a degradative fragment of the alpha-chain of hemoglobin. Our data suggest that the immunosuppressive T treatment of B10 mice induces a perturbation of erythrophagocytosis in spleens.
Fiebig
S
S
Division of Parasitology, Heinrich-Heine-University, Dusseldorf, Germany.
Schmitt-Wrede
H P
HP
Wunderlich
F
F
Stangier
J
J
Schiltz
E
E
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004151:Dipeptides / Q000378:metabolism*; D005260:Female; D006454:Hemoglobins / Q000378:metabolism*; D007165:Immunosuppression Therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D013154:Spleen / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology*
D004151:Dipeptides; D006454:Hemoglobins; C075870:methionylphenylalanine; D013739:Testosterone