title: Cross-reactivity of polyclonal serum antibodies generated against Cryptosporidium parvum oocysts.
abstract: Polyclonal antibodies raised against Cryptosporidium parvum oocysts were found to cross-react with Eimeria spp. oocyst antigens in an indirect immunofluorescence assay, and sera from Eimeria spp.-infected lambs reacted with some antigens from sonicated C. parvum oocysts (between 29 to 30 and 66 to 69 kDa) by Western blot (immunoblot). No cross-reaction was observed with cystozoites of Toxoplasma and Sarcocystis spp. These results show the existence of epitopes common to C. parvum and various Eimeria spp.
authors.affiliation: Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
authors.forename: L M
authors.initials: LM
authors.lastname: Ortega-Mora
authors.forename: J M
authors.initials: JM
authors.lastname: Troncoso
authors.forename: F A
authors.initials: FA
authors.lastname: Rojo-Vázquez
authors.forename: M
authors.initials: M
authors.lastname: Gómez-Bautista
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000032:analysis; D015153:Blotting, Western; D003429:Cross Reactions; D016785:Cryptosporidium parvum / Q000254:growth & development; D004539:Eimeria / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D011817:Rabbits; D012756:Sheep
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan