title: Simultaneous determination of paraquat and diquat in serum using capillary electrophoresis.
abstract: The use of capillary electrophoresis for simultaneous separation and detection of the two bipyridylium herbicides, paraquat and diquat, was investigated. Both herbicides were extracted from fortified sera with disposable ODS-silica cartridges. Separation was carried out using a capillary tube (50 microns i.d., 750 mm) of fused silica containing 10 mM glycine-HCl buffer (pH 3.0), 40 mM NaCl and 20% methanol as the carrier. Paraquat and diquat were completely separated in 10 min at an applied potential of 20 kV. On-column UV monitoring allowed detection of both herbicides simultaneously. The assay sensitivity was 0.05 micrograms/mL (signal-to-noise ratio, 2:1), which probably increases with increase in the sample volume of serum. Analytical recovery of both herbicides added to serum was about 97% at concentrations of 0.5-2.0 micrograms/mL.
authors.affiliation: Department of Legal Medicine, Kawasaki Medical School, Kurashiki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tomita
authors.forename: T
authors.initials: T
authors.lastname: Okuyama
authors.forename: Y
authors.initials: Y
authors.lastname: Nigo
mesh_terms: D004178:Diquat / Q000097:blood*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D000432:Methanol / Q000737:chemistry; D010269:Paraquat / Q000097:blood*; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D012965:Sodium Chloride; D004178:Diquat; D010269:Paraquat; D000432:Methanol