title: Determination of fluvoxamine in human plasma by high-performance liquid chromatography with fluorescence detection.
abstract: Fluvoxamine and nortriptyline, the assay internal standard, were extracted from plasma with ethyl acetate, then reacted with dansyl chloride. The derivatives were quantitated by isocratic reversed-phase high-performance liquid chromatography with fluorescence detection. The assay calibration range for fluvoxamine was 10-1000 ng/ml using a 1-ml plasma sample. Pooled plasma quality control sample relative recoveries at 25 and 250 ng/ml were 103 and 105%, respectively. Estimates of quality control inter-day precision during validation were less than or equal to 3% relative standard deviation. The assay was cross-validated with a gas chromatographic method and has been employed in therapeutic drug level monitoring.
authors.affiliation: Analytical Research and Development, Solvay Pharmaceuticals, Marietta, GA 30062.
authors.forename: R H
authors.initials: RH
authors.lastname: Pullen
authors.forename: A A
authors.initials: AA
authors.lastname: Fatmi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000737:chemistry; D016666:Fluvoxamine / Q000097:blood*; D006801:Humans; D009661:Nortriptyline / Q000097:blood; D011786:Quality Control; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D003619:Dansyl Compounds; D009661:Nortriptyline; D016666:Fluvoxamine