Evaluation of the amount of acyl-CoA elongases in leek (Allium porrum L) leaves.
Polyclonal antibodies have been raised against the acyl-CoA elongase purified from leek epidermal cells. The antibodies recognize the fractions containing the elongating activity after DEAE or Ultrogel chromatography and their response with the other fractions is very low. The immune complex is immunoprecipitable with Protein A-Sepharose. 1% of the solubilized proteins from leek epidermis microsomes are immunoprecipitated. The immunoprecipitate contains an elongating activity which is 86 +/- 20-times that of the unbound fraction.
Bessoule
J J
JJ
Institut de Biochimie Cellulaire et Neurochimie du CNRS, Université de Bordeaux, France.
Creach
A
A
Lessire
R
R
Cassagne
C
C
D051080:Acyl-Carrier Protein S-Malonyltransferase; D000217:Acyltransferases / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D008861:Microsomes / Q000201:enzymology; D010945:Plants, Edible / Q000201:enzymology*; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D000217:Acyltransferases; D051080:Acyl-Carrier Protein S-Malonyltransferase