title: A novel regulatory myosin light chain gene distinguishes pre-B cell subsets and is IL-7 inducible.
abstract: We describe a novel regulatory myosin light chain gene (termed precursor lymphocyte-specific regulatory light chain or PLRLC) that is expressed specifically in precursor B and T lymphocytes. PLRLC is the first example of a regulatory myosin light chain gene which displays specific expression in non-muscle cells. PLRLC is expressed in adult bone marrow derived normal and transformed pre-B cells; in the former, PLRLC expression levels are induced by the pre-B cell specific growth factor interleukin-7 (IL-7). PLRLC is not expressed in either transformed pre-B cells derived from fetal liver or in normal fetal liver pre-B clones grown in the presence of IL-7. Therefore this gene provides the first marker that clearly distinguishes these two pre-B subsets. Finally, several of the different PLRLC transcripts potentially encode regulatory myosin light chains with unique structural features. The unique distribution, regulation and structural features of the PLRLC gene products suggest an important role for PLRLC during lymphocyte development.
authors.affiliation: Howard Hughes Medical Institute, Boston, MA.
authors.forename: E M
authors.initials: EM
authors.lastname: Oltz
authors.forename: G D
authors.initials: GD
authors.lastname: Yancopoulos
authors.forename: M A
authors.initials: MA
authors.lastname: Morrow
authors.forename: A
authors.initials: A
authors.lastname: Rolink
authors.forename: G
authors.initials: G
authors.lastname: Lee
authors.forename: F
authors.initials: F
authors.lastname: Wong
authors.forename: K
authors.initials: K
authors.lastname: Kaplan
authors.forename: S
authors.initials: S
authors.lastname: Gillis
authors.forename: F
authors.initials: F
authors.lastname: Melchers
authors.forename: F W
authors.initials: FW
authors.lastname: Alt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000187:drug effects / Q000378:metabolism*; D001483:Base Sequence; D001854:Bone Marrow Cells; D002461:Cell Line, Transformed; D004247:DNA; D005786:Gene Expression Regulation; D015851:Interleukin-7 / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D005810:Multigene Family; D018994:Myosin Light Chains; D009218:Myosins / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D015851:Interleukin-7; D018994:Myosin Light Chains; C075642:Mylc2pl protein, mouse; D004247:DNA; D009218:Myosins