Inhibition of ACAT activity after 7-oxo-PGI2 treatment in cholesterol-fed rabbits.
It has been supposed that prostacyclin (PGI2) and its analogues have important antiatherogenic effects. The aim of this work was to test the effect of PGI2 and 7-oxo-PGI2- (a stable analogue of PGI2) (6) treatment on the acyl CoA: cholesterol-acyltransferase (ACAT) activity in the aortic wall of rabbits. The rabbits had been previously fed with cholesterol and treated with PGI2 and 7-oxo-PGI2 intravenously. Cholesterol feeding increased ACAT activity compared to the control group which was not fed with cholesterol: 16.84 nmol/mg prot./h and 10.03 nmol/mg prot./h, respectively. PGI2 treatment of the cholesterol fed group did not cause a significant decrease, while 7-oxo-PGI2 treatment significantly decreased aortic ACAT activity compared to the cholesterol-fed control group; 14.31 nmol/mg prot./h; 11.53 nmol/mg prot./h and 16.84 nmol/mg prot./h, respectively. The decrease found in the ACAT activity after PGI2 and 7-oxo-PGI2 treatment are new data for the protective effect of these agents against atherosclerosis.
Makáry
A
A
County Hospital of Kecskemét, Department of Pathology, Hungary.
Kolthay
E
E
Pataki
M
M
Petö
J
J
Kovács
G
G
Lusztig
G
G
D000818:Animals; D001011:Aorta / Q000201:enzymology; D002788:Cholesterol Esters / Q000096:biosynthesis; D002791:Cholesterol, Dietary / Q000378:metabolism*; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D008297:Male; D011817:Rabbits; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors*
D002788:Cholesterol Esters; D002791:Cholesterol, Dietary; C033705:7-oxoprostaglandin I2; D011464:Epoprostenol; D002785:Sterol O-Acyltransferase