title: Isolation of novel murine maternal mRNAs regulated by cytoplasmic polyadenylation.
abstract: The cytoplasmic polyadenylation element (CPE) is an AU-rich sequence in the 3'-untranslated region of many stored maternal mRNAs. The CPE directs the meiotic maturation-specific cytoplasmic polyadenylation and translational activation of these dormant mRNAs in Xenopus. The work presented here demonstrates that the CPE controls a similar regulation in mouse oocytes and utilizes the information to isolate novel maternal mRNAs by polymerase chain reaction (PCR). A degenerate CPE primer was used in an anchored PCR reaction with cDNAs from primary mouse oocytes. Clones were identified that contained the canonical polyadenylation signal AATAAA. A novel PCR test was then used to determine the polyadenylation state of the respective mRNAs before and after meiotic maturation. Two mRNAs, OM-1 and OM-2, are cytoplasmically polyadenylated upon maturation. Another mRNA is not polyadenylated during maturation, although it contains multiple CPE-like elements, indicating that this sequence element is not sufficient for adenylation during this time. Microinjection into primary oocytes of antisense oligodeoxynucleotides directed against OM-1 destroys the mRNA but does not appear to interfere with maturation in vitro. These experiments identify two novel maternal mRNAs and establish a simple strategy for isolating other maternal messages that control meiotic maturation, fertilization, and early mouse development.
authors.affiliation: Department of Pharmacology, State University of New York, Stony Brook 11794-8651.
authors.forename: F J
authors.initials: FJ
authors.lastname: Sallés
authors.forename: A L
authors.initials: AL
authors.lastname: Darrow
authors.forename: M L
authors.initials: ML
authors.lastname: O'Connell
authors.forename: S
authors.initials: S
authors.lastname: Strickland
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism*; D004247:DNA; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D011061:Poly A / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA