title: Characterization of the heredity of kininogen deficiency in brown Norway Katholiek strain rats.
abstract: Brown Norway rat strain has been studied for mode of inheritance of its congenital deficiency in plasma high molecular weight (HMW)-kininogen and low molecular weight (LMW)-kininogen, and low plasma level of prekallikrein. We examined the genetics of the deficiency by performing a mixed breeding experiment between B/N-Katholiek (B/N-Ka, deficient) and B/N-Kitasato (B/N-Ki, normal) strains. Incidence of the deficiency was judged by the plasma level of HMW-kininogen. Plasma level of HMW-kininogen was around 50% of the normal level in all F1 generations of the hybrid between male B/N-Ka and female B/N-Ki (Exp. 1), and between female B/N-Ka and male B/N-Ki (Exp. 2). Incidence of deficiency (plasma HMW-kininogen level less than 5%) in Exp. 1 was 23.8% in male F2 and 20.0% in female F2 generations. By Exp. 2 also the incidence was 25.0% in male and 30.0% in female F2 generations. There was no significant difference of the incidence between the two experiments or sexes. These results indicate the inheritance of the kininogen-deficiency to be Mendelian autosomal recessive, the same as for the reported cases of human kininogen deficiency. Gel filtration study suggests that prekallikrein in the B/N-Ka plasma may be free form, while that in the B/N-Ki plasma may form complex with HMW-kininogen.
authors.affiliation: Department of Pharmacology, School of Pharmac. Sci., Kitasato University, Tokyo, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Fujie
authors.forename: M
authors.initials: M
authors.lastname: Mita
authors.forename: S
authors.initials: S
authors.lastname: Oh-ishi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002850:Chromatography, Gel; D005260:Female; D005808:Genes, Recessive; D007704:Kininogens / Q000097:blood / Q000172:deficiency* / Q000235:genetics; D008297:Male; D010375:Pedigree; D011288:Prekallikrein / Q000378:metabolism; D051381:Rats; D011922:Rats, Mutant Strains
chemical_list: D007704:Kininogens; D011288:Prekallikrein