title: Aspergillus niger G proteins: subcellular localization.
abstract: Aspergillus niger postmitochondrial fraction, which contains high GTPase activity and high GTP binding capacity, has been subjected to subcellular fractionation on a sucrose gradient. A cytosolic and four membranous populations have been separated according to their relative density. The main difficulty has been the characterization of the plasma membrane of the fungus. This fraction, which does not contain any typical enzyme, has been identified after iodination of the outer proteins of protoplasts from A. niger. The immunological detection has shown the occurrence of cytosolic G proteins and membranous small G proteins located not only in the plasma membrane but also in the membranes of the endoplasmic reticulum.
authors.affiliation: Laboratoire de Biochimie des Membranes, Université Lyon I, Villeurbanne, France.
authors.forename: X
authors.initials: X
authors.lastname: Mérit
authors.forename: J
authors.initials: J
authors.lastname: Frot-Coutaz
authors.forename: R
authors.initials: R
authors.lastname: Got
authors.forename: R
authors.initials: R
authors.lastname: Létoublon
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry; D004721:Endoplasmic Reticulum / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000302:isolation & purification*; D013347:Subcellular Fractions / Q000737:chemistry
chemical_list: D019204:GTP-Binding Proteins