A novel low-density lipoprotein with large amounts of phospholipid found in the egg yolk of crustacea sand crayfish Ibacus ciliatus: its function as vitellogenin-degrading proteinase.
Low-density lipoprotein (LDL) with large amounts of phospholipid but not triacylglycerol was isolated from the egg yolk of crustacea sand crayfish Ibacus ciliatus as well as lipovitellin. LDL possessed vitellogenin-degrading proteinase activity. Hemolymph vitellogenin was degraded by incubating with LDL at pH 4.5 for 72 hr at 35 degrees C and apolipoprotein profiles of vitellogenin degraded by LDL were very similar to those of lipovitellin in the egg.
Komatsu
M
M
Laboratory of Food Chemistry, Faculty of Fisheries, Kagoshima University, Japan.
Ando
S
S
D000818:Animals; D003400:Astacoidea; D002784:Cholesterol / Q000032:analysis; D004530:Egg Yolk; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D006458:Hemolymph / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D010063:Ovum / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis*; D013379:Substrate Specificity; D014280:Triglycerides / Q000032:analysis; D014819:Vitellogenins / Q000378:metabolism*
D008077:Lipoproteins, LDL; D010743:Phospholipids; D014280:Triglycerides; D014819:Vitellogenins; D002784:Cholesterol; D010450:Endopeptidases