title: An improved CPG support for the synthesis of 3'-amine-tailed oligonucleotides.
abstract: A new controlled-pore glass (CPG) support is described that allows for the direct synthesis of oligonucleotides bearing a 3'-aminohexyl tail. This solid support (AH-CPG) exhibits superior performance as compared to a commercially available 3'-amine CPG. The AH-CPG is prepared from 6-aminohexan-1-ol with a unique protecting group for the amine that also functions as the site of attachment to the CPG. A 3'-amine-tailed oligodeoxynucleotide (ODN) was prepared from this support using standard phosphoramidite coupling and deprotection conditions. The 3'-amine-tailed ODN was subsequently modified with an acridinylpropionic acid tetrafluorophenyl ester. Facile synthesis of the AH-CPG and the stability of the deprotected product makes this functionalized solid support especially useful for preparation of oligonucleotides bearing 3'-amine tails and other modifications.
authors.affiliation: MicroProbe Corporation, Bothell, Washington 98021.
authors.forename: C R
authors.initials: CR
authors.lastname: Petrie
authors.forename: M W
authors.initials: MW
authors.lastname: Reed
authors.forename: A D
authors.initials: AD
authors.lastname: Adams
authors.forename: R B
authors.initials: RB
authors.lastname: Meyer
mesh_terms: D000166:Acridines / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005898:Glass; D009841:Oligonucleotides / Q000138:chemical synthesis*
chemical_list: D000166:Acridines; D009841:Oligonucleotides