Rapid three-dimensional reconstruction at the light microscopic level and a technique for re-embedding the same semithin sections for electron microscopic examination.
Rapid three-dimensional reconstruction of serial sections at the light microscopic and ultrastructural levels was accomplished using a two-step technique. Fixed specimens were embedded in Epon and 1 microns sections were cut and placed on glass slides. One of every four sections was drawn onto transparency film for rapid three-dimensional reconstruction. The semi-thin sections were re-embedded in Epon and sectioned at 90 nm for examination in the electron microscopy.
Pignot-Paintrand
I
I
Laboratory of Biology of Reproduction and Development, University of Paris-South Central Hospital of Bicêtre, Le Kremlin Bicetre, France.
Bressac
C
C
D000818:Animals; D004330:Drosophila; D004853:Epoxy Resins; D005260:Female; D005836:Genitalia, Female / Q000648:ultrastructure; D016591:Histocytological Preparation Techniques; D008297:Male; D008853:Microscopy; D008854:Microscopy, Electron; D008867:Microtomy / Q000379:methods*; D013094:Spermatozoa / Q000648:ultrastructure; D016610:Tissue Embedding / Q000379:methods*
D004853:Epoxy Resins; C004875:EPON