title: A family of human cdc2-related protein kinases.
abstract: The p34cdc2 protein kinase is known to regulate important transitions in the eukaryotic cell cycle. We have identified 10 human protein kinases based on their structural relation to p34cdc2. Seven of these kinases are novel and the products of five share greater than 50% amino acid sequence identity with p34cdc2. The seven novel genes are broadly expressed in human cell lines and tissues with each displaying some cell type or tissue specificity. The cdk3 gene, like cdc2 and cdk2, can complement cdc28 mutants of Saccharomyces cerevisiae, suggesting that all three of these protein kinases can play roles in the regulation of the mammalian cell cycle. The identification of a large family of cdc2-related kinases opens the possibility of combinatorial regulation of the cell cycle together with the emerging large family of cyclins.
authors.affiliation: Massachusetts General Hospital Cancer Center, Charlestown 02129.
authors.forename: M
authors.initials: M
authors.lastname: Meyerson
authors.forename: G H
authors.initials: GH
authors.lastname: Enders
authors.forename: C L
authors.initials: CL
authors.lastname: Wu
authors.forename: L K
authors.initials: LK
authors.lastname: Su
authors.forename: C
authors.initials: C
authors.lastname: Gorka
authors.forename: C
authors.initials: C
authors.lastname: Nelson
authors.forename: E
authors.initials: E
authors.lastname: Harlow
authors.forename: L H
authors.initials: LH
authors.lastname: Tsai
mesh_terms: D000595:Amino Acid Sequence; D016203:CDC2 Protein Kinase / Q000235:genetics*; D002453:Cell Cycle; D002460:Cell Line; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D016203:CDC2 Protein Kinase