Glucose uptake by rat erythroid cells: the effects of erythropoietin and dexamethasone.
Erythropoietin (Ep) stimulated glucose uptake by erythroid progenitor cells in the liver of fetal rats, as measured by [3H]2-deoxy-D-glucose uptake. This dose-dependent stimulation was maximal at 0.2 U/ml Ep and decreased during cell differentiation. Dexamethasone (DEX) inhibited glucose uptake by erythroid progenitors; this dose-dependent effect was maximal at 10(-7) M DEX. Ep partly counteracted the inhibitory effect of DEX. This antagonism may contribute to the overall antagonism between Ep and glucocorticoids in the development of erythroid tissue in vivo and in vitro.
Billat
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C
Laboratoire de Biochimie, UFR Sciences, Université de Reims Champagne-Ardenne, France.
Jacquot
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R
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000378:metabolism*; D004921:Erythropoietin / Q000494:pharmacology; D051381:Rats; D013997:Time Factors
D004921:Erythropoietin; D003907:Dexamethasone; D003847:Deoxyglucose