Identification and partial characterization of the salmon calcitonin/CGRP gene by polymerase chain reaction.
The polymerase chain reaction was used to amplify sequences encoding calcitonin and CGRP in the genomic DNA of salmon. Amplification products of the expected length were cloned and sequenced. In this way a new CGRP-coding sequence was identified. The new sequence and the known salmon calcitonin-coding sequence were shown to be part of one gene, implying that alternative gene expression takes place in fish.
Jansz
H S
HS
Laboratory for Physiological Chemistry, University of Utrecht, The Netherlands.
Zandberg
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002116:Calcitonin / Q000235:genetics*; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D006801:Humans; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012474:Salmon; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000502:physiology
D009838:Oligodeoxyribonucleotides; D002116:Calcitonin; D004247:DNA; D015740:Calcitonin Gene-Related Peptide