Herpes simplex virus type I (HSV I)-induced multifocal central nervous system (CNS) demyelination in mice.
Multifocal central nervous system (CNS) demyelination develops in the brains of SJL/J, PL/J, and A/J mice following lip inoculation with a specific strain of herpes simplex virus I (HSV I). The lesions in all three inbred strains of mice share similar characteristics including demyelination, relative preservation of axons, and a mononuclear cell (MNC) infiltrate. The lesions, developing during the early phase of demyelination, also appear sequentially in the CNS (trigeminal root entry zone of the brainstem greater than cerebellum greater than cerebral hemispheres) of all three strains of mice but differ in the time of their initial appearance following infection as well as their morphology. In SJL/J mice, new areas of demyelination are observed for only 24 days following lip inoculation with virus. Late stage multifocal CNS demyelination persists throughout 28 weeks postinoculation (pi) in PL/J mice while in A/J mice the development of new areas of demyelination are restricted to 8 weeks pi. Although mononuclear inflammatory cells are present in the new areas of demyelination in either PL/J or A/J mice, viral antigens are not detected in the CNS beyond 12 days pi. In contrast, in situ hybridization studies using 35S-cDNA HSV probes and performed beyond day 12 pi identify probe-positive cells central to a number of the multifocal CNS demyelinating lesions in A/J mice. Results from studies with inbred and congenic strains of mice indicate that the major histocompatibility complex (H-2) does not determine the development of multifocal CNS demyelination following lip inoculation with HSV I but does influence the morphological appearance of the lesions that do develop.
Kastrukoff
L F
LF
Division of Neurology, University of British Columbia, Vancouver, Canada.
Lau
A S
AS
Leung
G Y
GY
Walker
D G
DG
Thomas
E E
EE
Walker
D
D
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D001927:Brain Diseases / Q000382:microbiology* / Q000473:pathology; D004279:DNA, Viral / Q000032:analysis; D003711:Demyelinating Diseases / Q000382:microbiology* / Q000473:pathology; D006561:Herpes Simplex / Q000473:pathology; D008285:Major Histocompatibility Complex / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains
D000956:Antigens, Viral; D004279:DNA, Viral