A similar distribution of gonadotropin isohormones is maintained in the pituitary throughout sexual maturation in the heifer.
Our working hypotheses for this study were that 1) the profile of intrapituitary LH and FSH isoforms would be shifted toward acidic forms as sexual maturation progresses in the bovine female; and 2) concentration of 17 beta-estradiol (E2) in circulation during sexual maturation would be a major factor modulating the percentage of the more acidic isoforms. In addition, the biological-immunoreactive (B:I) ratios of each isoform of LH were evaluated at selected stages of sexual maturation. Heifers (7 mo of age) were assigned to one of three treatment groups: 1) ovariectomized (OVX; n = 16); 2) OVX and administered E2 (OVXE; n = 16); or 3) ovary-intact (INTACT; n = 14). Pituitaries were collected from heifers in each group at an estimated 120 days (prepubertal) of 25 days before puberty (peripubertal). A fourth group of 6 heifers remained intact (postpubertal INTACT) to determine time of puberty during the experimental period. Pituitaries of heifers assigned to the postpubertal INTACT group were collected during the follicular phase of the first or second estrous cycle postpuberty. Pituitaries were used for determination of relative amounts of gonadotropin isohormones. Tissue extracts of the pituitaries were chromatofocused on pH 10.5-4.0 gradients. The LH of all pituitaries resolved into thirteen isoforms that were designated isoforms A-L, and S, with isoform A the most basic form. Isoforms F and G (basic pH range) were the predominant isoforms of each chromatofocusing profile and comprised 50-60% of the immunoreactive LH. Isoforms J and K were the major isoforms eluting in the acidic pH range.(ABSTRACT TRUNCATED AT 250 WORDS)
Stumpf
T T
TT
Department of Animal Science, University of Nebraska-Lincoln 68583-0908.
Roberson
M S
MS
Wolfe
M W
MW
Zalesky
D D
DD
Cupp
A S
AS
Werth
L A
LA
Kojima
N
N
Hejl
K
K
Kittok
R J
RJ
Grotjan
H E
HE
D000818:Animals; D002417:Cattle / Q000254:growth & development* / Q000378:metabolism; D004958:Estradiol / Q000097:blood / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007986:Luteinizing Hormone / Q000378:metabolism*; D009929:Organ Size; D010052:Ovariectomy; D010902:Pituitary Gland / Q000254:growth & development / Q000378:metabolism*
D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone