Folate receptor allows cells to grow in low concentrations of 5-methyltetrahydrofolate.
The folate receptor mediates the uptake of 5-methyltetrahydrofolate in certain cultured cells by a process called potocytosis. When these cells are grown in physiological concentrations of folate, the receptor increases the efficiency of vitamin uptake by 30-fold. We now show that PAM 212 cells, a mouse keratinocyte cell line, are unable to grow in 1 nM 5-methyltetrahydrofolate unless they express a functional folate receptor. These results suggest that under certain conditions, tissue cells in the body may depend on the folate receptor to obtain enough 5-methyltetrahydrofolate for growth.
Matsue
H
H
Department of Dermatology, University of Texas Southwestern Medical Center, Dallas 75235-9039.
Rothberg
K G
KG
Takashima
A
A
Kamen
B A
BA
Anderson
R G
RG
Lacey
S W
SW
D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000502:physiology*; D002455:Cell Division; D002478:Cells, Cultured; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins / Q000378:metabolism; D013763:Tetrahydrofolates / Q000378:metabolism*; D014162:Transfection
D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D013763:Tetrahydrofolates; D005492:Folic Acid; C005984:5-methyltetrahydrofolate