title: Synthesis of N-[6-(ethylenedioxy)hexyl]biotinamide: a biotinyl aldehyde precursor for labeling hydrazine-modified biomolecules.
abstract: Synthetic approaches for obtaining biotinyl aldehydes are described. While such aldehydes have limited shelflife, the acetal derivative, N-[6-(ethylenedioxy)hexyl]biotinamide, IX, was found to be indefinitely stable upon storage at -20 degrees C. Mild acid hydrolysis conveniently unmasks the aldehyde, which can then be used to label hydrazine-tagged biomolecules.
authors.affiliation: SISKA Diagnostics, Inc., San Diego, California.
authors.forename: G F
authors.initials: GF
authors.lastname: Musso
authors.forename: G R
authors.initials: GR
authors.lastname: Scarlato
authors.forename: S S
authors.initials: SS
authors.lastname: Ghosh
mesh_terms: D001710:Biotin / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D006834:Hydrazines / Q000737:chemistry*; D006868:Hydrolysis; D013696:Temperature
chemical_list: D006834:Hydrazines; C075482:N-(6-(ethylenedioxy)hexyl)biotinamide; C029424:hydrazine; D001710:Biotin