Comparison of a vaccine strain and field isolates of avian reovirus by T1-oligonucleotide mapping.
Total RNA of eight avian reovirus isolates and the S1133 strain were compared by RNase T1-oligonucleotide mapping. The viruses were propagated in Vero cell cultures, and viral genomes were extracted from purified virions for comparison. Pairwise comparisons of the oligonucleotide maps showed genetic variation among reovirus isolates ranging from 78% to 99%. The T1 fingerprints of the RNA of isolates 1103, 724, 615, and 684 differed slightly from the standard S1133 strain, suggesting that the vaccine strain might have changed and became part of the circulating reoviruses. In contrast, when compared with the vaccine strain, isolates 902, 644, and 6207 showed greater differences in the fingerprint pattern. This genomic diversity may be due to the differences in immunological status of the affected avian population and/or due to simultaneous coinfection with different reovirus strains.
Rekik
M R
MR
Université de Montréal, Faculté de Médecine Vétérinaire, St-Hyacinthe, Québec, Canada.
Silim
A
A
D000818:Animals; D009841:Oligonucleotides / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012087:Reoviridae / Q000145:classification / Q000235:genetics*; D015203:Reproducibility of Results; D014613:Vaccines, Attenuated / Q000235:genetics; D014709:Vero Cells; D014765:Viral Vaccines / Q000235:genetics*
D009841:Oligonucleotides; D012367:RNA, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines