title: The distribution of 5-methylcytosine in the nuclear genome of plants.
abstract: We have determined the 5-methylcytosine (5mC) content in high molecular weight DNA, from two dicot (tobacco and pea) and two monocot (wheat and maize) plant species, fractionated according to base composition. The results show that the proportion of 5mC in the genomic fractions increases linearly with their guanine + cytosine (G + C) content while the proportion of non-methylated cytosine remains almost constant. This can be interpreted as a consequence of a difference in mutation pressure related to spontaneous deamination of 5mC to thymine between the different compartments of plant genomes.
authors.affiliation: Dpto. de Protección Vegetal, INIA, Madrid, Spain.
authors.forename: L M
authors.initials: LM
authors.lastname: Montero
authors.forename: J
authors.initials: J
authors.lastname: Filipski
authors.forename: P
authors.initials: P
authors.lastname: Gil
authors.forename: J
authors.initials: J
authors.lastname: Capel
authors.forename: J M
authors.initials: JM
authors.lastname: Martínez-Zapater
authors.forename: J
authors.initials: J
authors.lastname: Salinas
mesh_terms: D044503:5-Methylcytosine; D001482:Base Composition / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D007887:Fabaceae / Q000235:genetics*; D016678:Genome; D010946:Plants, Medicinal; D010947:Plants, Toxic; D014026:Nicotiana / Q000235:genetics*; D014908:Triticum / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
chemical_list: D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA