title: [Use of molecular biology in the epidemiological characterization of Neisseria gonorrhoeae].
abstract: This paper describes current methods useful to define molecular markers from a collection of Neisseria gonorrhoeae strains. The procedures of auxotyping, serotyping and plasmid profiling led to the obtention of 10 auxotypes, 19 serotypes and five plasmid types (including beta-lactamase plasmids) among 41 gonococci studied. Twelve patterns of antimicrobial resistance were determined as well, through in vitro susceptibility testing by agar dilution. These tools in conjunction, offer the possibility to study gonorrhea in a dynamic fashion from an epidemiologic perspective. Furthermore, they have allowed us to establish a gonococcal reference laboratory in our institution.
authors.affiliation: Departamento de Bacteriología, Centro de Investigaciones sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, México.
authors.forename: E
authors.initials: E
authors.lastname: Náder-García
authors.forename: V A
authors.initials: VA
authors.lastname: Mondragón-Jaimes
authors.forename: C J
authors.initials: CJ
authors.lastname: Conde-González
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D006069:Gonorrhea / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000187:drug effects* / Q000235:genetics; D010957:Plasmids; D012703:Serotyping
chemical_list: D000900:Anti-Bacterial Agents
vernacular_title: Empleo de la biología molecular para la caracterización epidemiológica de Neisseria gonorrhoeae.