Mixed cultivation of Trichoderma reesei and Aspergillus ochraceus for improved cellulase production.
Aspergillus ochraceus IMI 317911 was screened to be a high cellobiase producing strain (2.40 IU/ml). The mixed batch cultivation of Trichoderma reesei QM 9414/Rut C-30 and A. ochraceus IMI 317911 resulted in a balanced enzyme as compared to singly grown cultures. The milled rice straw (6%, w/v) as carbon source was found suitable for production of cellulases resulting in a Filter Paper Activity (FPA) of 1.83 IU/ml and a cellobiase production of 1.63 IU/ml after 7 days of stirred cultivation. An overall cellulase productivity of 10.89 IU/l/h and an enzyme ratio to cellobiase of 1.12 was achieved.
Chadha
B S
BS
Department of Microbiology, Punjab Agricultural University, Ludhiana, India.
Garcha
H S
HS
D001235:Aspergillus ochraceus / Q000201:enzymology / Q000254:growth & development*; D002244:Carbon / Q000378:metabolism; D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose / Q000378:metabolism; D005285:Fermentation; D009172:Mycology / Q000379:methods; D012275:Oryza / Q000378:metabolism; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology / Q000254:growth & development*; D001617:beta-Glucosidase / Q000096:biosynthesis
D002244:Carbon; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase