title: Bactericidal activities of ofloxacin and its optically active isomer (DR-3355) on non-growing cells of Escherichia coli and Pseudomonas aeruginosa.
abstract: In this paper the bactericidal activities of ofloxacin and its optically active derivative, DR-3355, against non-growing cells of Escherichia coli and Pseudomonas aeruginosa are described. E. coli and P. aeruginosa were killed rapidly by ofloxacin and DR-3355. After treatment with these quinolones, the resting cells of E. coli and P. aeruginosa became plasmolyzed, with apparent cytoplasmic shrinkage without filamentation. Membrane-bound intracellular vacuoles and disruption of the cell envelope were also observed, resulting in extrusion of the cytoplasmic contents. These results indicate that non-growing cells of E. coli and P. aeruginosa were susceptible to ofloxacin and DR-3355, as were logarithmically growing cells.
authors.affiliation: Tokyo R & D Center, Daiichi Pharmaceutical Co., Ltd., Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Otsuki
authors.forename: T
authors.initials: T
authors.lastname: Nishino
mesh_terms: D002453:Cell Cycle; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D015242:Ofloxacin / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D015242:Ofloxacin