title: Conserved signals in the 5' flanking region of eukaryotic nuclear tRNA genes.
abstract: The statistical analysis of 5' flanking regions of eukaryotic tRNA genes was done. The analysis of nucleotides in the sequence of fungi and invertebrates showed a high content of A and T in the flanking regions versus coding regions where G and C dominate. In contrast to these results in vertebrates sequences the preferences of any nucleotide in flanking regions was not observed. The analysis of tetrads showed five conserved signals: TTGT, (T/A)(T/A)ATA, A(C/T)(C/A)A in the tRNA genes of fungi, (A/T)TGA of invertebrates and (A/T)GAG of vertebrates. The analysis of 3' flanking regions did not show any conserved signals except well known poly-T tracks.
authors.affiliation: Poznań University, Department of Biopolymer Biochemistry, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Makałowski
authors.forename: J
authors.initials: J
authors.lastname: Augustyniak
mesh_terms: D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence / Q000235:genetics; D005057:Eukaryotic Cells; D005658:Fungi / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D007448:Invertebrates / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D014714:Vertebrates / Q000235:genetics
chemical_list: D012343:RNA, Transfer