title: Relation between the insulin receptor number in cells, autophosphorylation and insulin-stimulated Ras.GTP formation.
abstract: We showed previously that upon insulin stimulation of an insulin receptor overexpressing cell line, most of the p21ras was rapidly converted into the GTP bound state (Burgering, B. M. T., Medema, R. H., Maassen, J. A., Van de Wetering, M. L., Van der Eb, A. J., McCormick, F., and Bos, J. L. (1991) EMBO J. 10, 1103-1109). To determine whether this process also occurs in cells expressing physiologically relevant numbers of insulin receptors, insulin stimulated Ras.GTP formation was quantitated in Chinese hamster ovary (CHO)-derived cell lines expressing varying numbers of insulin receptors. In the parental CHO9 cells, expressing only 5.10(3) insulin receptors, insulin stimulation for 3 min increased Ras.GTP levels with 10%. Upon increasing the number of insulin receptors in these cells, Ras.GTP levels increased almost proportionally until a plateau value of 60% is reached at high receptor numbers. These data show that receptor overexpression is not a prerequisite for insulin-stimulated Ras.GTP formation. The yield of Ras.GTP generated is 0.2-1.0 mol/mol autophosphorylated insulin receptor in CHO9- and NIH3T3-derived cell lines, respectively. These values argue against signal-amplifying processes between the insulin receptor and p21ras. To determine whether receptor autophosphorylation is required for Ras.GTP formation, NIH3T3 cells overexpressing insulin receptors were stimulated with a monoclonal antibody which activates the receptor and subsequent glucose transport without inducing detectable autophosphorylation. Also, CHO cells expressing the mutant Ser1200 receptor, which has markedly impaired tyrosyl autophosphorylation but is capable of mediating insulin-stimulated metabolic effects in CHO cells, were used. In both cases, no Ras.GTP formation was observed. Furthermore, Rat-1-derived cell lines expressing mutant p21ras, which is permanently in the active GTP-bound form, still responded to insulin by increasing the glucose uptake. These results support our hypothesis that Ras.GTP formation is activated by the tyrosyl-phosphorylated insulin receptor and suggest that an active Ras.GTP complex does not mediate metabolic signaling.
authors.affiliation: Laboratory of Protein Synthesis and Hormone Regulation, Sylvius Laboratory, Leiden, The Netherlands.
authors.forename: A P
authors.initials: AP
authors.lastname: Osterop
authors.forename: R H
authors.initials: RH
authors.lastname: Medema
authors.forename: J L
authors.initials: JL
authors.lastname: Bos
authors.forename: G C
authors.initials: GC
authors.lastname: vd Zon
authors.forename: D E
authors.initials: DE
authors.lastname: Moller
authors.forename: J S
authors.initials: JS
authors.lastname: Flier
authors.forename: W
authors.initials: W
authors.lastname: Möller
authors.forename: J A
authors.initials: JA
authors.lastname: Maassen
mesh_terms: D016475:3T3 Cells; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism; D020690:GTPase-Activating Proteins; D006003:Glycogen / Q000096:biosynthesis; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D009154:Mutation; D015689:Oncogene Protein p21(ras) / Q000378:metabolism*; D010766:Phosphorylation; D014176:Protein Biosynthesis; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D020703:ras GTPase-Activating Proteins
chemical_list: D020690:GTPase-Activating Proteins; D006601:Hexoses; D007328:Insulin; D020703:ras GTPase-Activating Proteins; D006160:Guanosine Triphosphate; D006003:Glycogen; D004247:DNA; D003847:Deoxyglucose; D011972:Receptor, Insulin; D015689:Oncogene Protein p21(ras); D013936:Thymidine