Influence of a cholesterol rich diet in rabbits on the formation of PGI2 and TXA2.
In the study was investigated whether the formation of prostanoids is changed in the different regions of aorta or in clotting whole blood in dependence on development of atherosclerosis. For this question New Zealand rabbits were fed for different periods with a cholesterol rich diet (0.5%). At the end of the different dietary periods the animals were killed and the following parameters estimated: blood: levels of total cholesterol, HDLcholesterol, VLDLcholesterol, cholesterol in the beta-migrating lipoprotein fraction, serum lipid peroxides, TXB2 formation capacity of clotting whole blood; aorta: surface of intima covered with fatty streaks, free and esterified cholesterol, triglycerides, collagen, formation of 6-keto-PGF1a and TXB2 by abdominal and thoracic aortas. The lipid parameter demonstrated a relatively strong correlation with the duration of cholesterol rich diet or the macroscopically detectable atherosclerosis, but the prostanoid formation remained unchanged.
Beitz
J
J
Department of Pharmacology and Toxicology, Martin Luther University Halle-Wittenberg, FRG.
Beitz
A
A
Giessler
C
C
Mentz
P
P
Orekhov
A N
AN
Andreeva
E R
ER
Mest
H J
HJ
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D003324:Coronary Artery Disease / Q000097:blood; D011464:Epoprostenol / Q000096:biosynthesis*; D008074:Lipoproteins / Q000097:blood; D011817:Rabbits; D013928:Thromboxane A2 / Q000096:biosynthesis*
D002791:Cholesterol, Dietary; D008074:Lipoproteins; D013928:Thromboxane A2; D011464:Epoprostenol