title: Changes in alanine and glutamine transport during rat red blood cell maturation.
abstract: Alanine and glutamine transport have been studied during red blood cell maturation in the rat. Kinetic parameters of Na(+)-dependent L-alanine transport were: Km 0.43 and 1.88 mM and Vmax 158 and 45 nmoles/ml ICW/min for reticulocytes and erythrocytes, respectively. During red cell maturation in the rat there is a loss of capacity and affinity of the system ASC for L-alanine transport. The values for Na(+)-dependent L-glutamine transport in reticulocytes were Km 0.51 mM and Vmax 157 nmoles/ml ICW/min. On the other hand, a total loss of L-glutamine transport mediated by both N and ASC systems is demonstrated in mature red cells. This seems to indicate that during rat red cell maturation the system N disappears. Furthermore, the system ASC specificity in mature cells changes, and glutamine enters the red cell by non-mediated diffusion processes.
authors.affiliation: Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Felipe
authors.forename: O
authors.initials: O
authors.lastname: Viñas
authors.forename: X
authors.initials: X
authors.lastname: Remesar
mesh_terms: D000409:Alanine / Q000097:blood*; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D004905:Erythrocyte Aging / Q000502:physiology; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005973:Glutamine / Q000097:blood*; D007700:Kinetics; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000502:physiology
chemical_list: D005973:Glutamine; D012964:Sodium; D000409:Alanine