Increased apolipoprotein A-IV in rat mesenteric lymph after lipid meal acts as a physiological signal for satiation.
Chylomicron transport and apolipoprotein A-IV (apo A-IV) output in mesenteric lymph increases significantly after a lipid meal. Mesenteric lymph collected from lymph-fistula rats after lipid infusion exerted an anorectic effect on 24 h fasted rats after administration through chronically indwelling right atrial catheter. Lymph before lipid infusion and lymph collected from a rat fed lipid plus Pluronic L-81 (a potent inhibitor of chylomicron formation) had no anorectic effect. Chylomicron and apo A-IV-rich lymph lost its anorectic effect after specific immunoprecipitation of apo A-IV by a monospecific antibody. Intravenous infusion of the same amount of purified apo A-IV as in chylous lymph 6-8 h after a lipid meal suppressed food intake. This unique physiological function of apo A-IV is not shared by apolipoprotein A-I. It is proposed that apo A-IV is a circulating signal released in response to fat feeding and that it is likely to mediate the anorectic effect of a lipid meal.
Fujimoto
K
K
Department of Physiology, Louisiana State University Medical Center, Shreveport 71130.
Cardelli
J A
JA
Tso
P
P
D000704:Analysis of Variance; D000818:Animals; D001053:Apolipoproteins / Q000378:metabolism; D001054:Apolipoproteins A / Q000502:physiology*; D004041:Dietary Fats; D004326:Drinking; D004435:Eating / Q000502:physiology; D006706:Homeostasis; D008196:Lymph / Q000502:physiology*; D008297:Male; D009043:Motor Activity; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012527:Satiation / Q000502:physiology*; D014280:Triglycerides / Q000378:metabolism
D001053:Apolipoproteins; D001054:Apolipoproteins A; D004041:Dietary Fats; D010743:Phospholipids; D014280:Triglycerides; C022205:apolipoprotein A-IV