title: Corneal epithelial healing under contact lenses. Quantitative analysis in the rabbit.
abstract: The rate of corneal epithelial healing was determined as a function of the oxygen level present at the tear-cornea interface. Oxygen levels of 1.77, 2.3, 5.3, 8.55, 10.12, and 16.45% were created over the rabbit cornea with tarsorrhaphy and various contact lenses. Full depth epithelial defects of 4.5 mm diameter were created in 6 animals for each test condition with the fellow eye serving as a control and healing in air (21.09% oxygen). The defect (radius) was measured at 24-h intervals until healing occurred. Statistically significant (p less than 0.05, paired Student t-test) delays in healing were found with oxygen levels of 8.55% and below. These delays were most profound over the initial 24 h. The healing rate with tarsorrhaphy, at the 1.77% oxygen level, over the first 24 h was 5.2 microns/h radius, only 15.2% of the paired control, 34.0 microns/h radius, healing in air (21.09% oxygen). Healing rates over the initial 24 h under contact lenses allowing 2.3, 5.3, 8.55, 10.12, and 16.45% oxygen were 6.1, 23.5, 26.5, 43.0, and 45.0 microns/h in radius, respectively.
authors.affiliation: Department of Ophthalmology, Ohio State University, Columbus.
authors.forename: T F
authors.initials: TF
authors.lastname: Mauger
authors.forename: R M
authors.initials: RM
authors.lastname: Hill
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D003263:Contact Lenses, Hydrophilic; D003315:Cornea / Q000378:metabolism / Q000502:physiology*; D003316:Corneal Diseases / Q000503:physiopathology; D004848:Epithelium / Q000378:metabolism / Q000502:physiology; D005143:Eyelids / Q000601:surgery; D010100:Oxygen / Q000378:metabolism; D011817:Rabbits; D014945:Wound Healing / Q000502:physiology*
chemical_list: D010100:Oxygen