A single serine:pyruvate aminotransferase gene on rat chromosome 9q34-q36.
It was found in our previous study (Oda et al., 1990. J. Biol. Chem. 265: 7513-7519) that in the rat two mRNAs encoding mitochondrial and peroxisomal serine:pyruvate aminotransferase (SPT/AGT) are formed from a single SPT/AGT gene through alternative transcription initiation in exon 1. In an attempt to analyze the mechanisms underlying this unique phenomenon, we have isolated genomic clones harboring the entire rat SPT/AGT gene. In the present study, the location of the rat SPT/AGT gene was determined to be in the q34-q36 region of chromosome 9 by fluorescence in situ hybridization. Southern blot analysis of rat genomic DNA revealed an allelic BamHI restriction fragment length polymorphism among three different inbred rat strains. These results indicated that a single copy SPT/AGT gene is located on chromosome 9q34-q36 in the rat genome. This locus has been assigned the gene symbol Spat.
Mori
M
M
Institute of Laboratory Animals, Faculty of Medicine, Kyoto University, Japan.
Oda
T
T
Nishiyama
K
K
Serikawa
T
T
Yamada
J
J
Ichiyama
A
A
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D000637:Transaminases / Q000235:genetics*
D015342:DNA Probes; D004247:DNA; D000637:Transaminases; C022638:serine-pyruvate aminotransferase