Identification of GATA-1 and NF-E2 binding sites in the flanking regions of the human alpha-globin genes.
Some of the elements involved in the erythroid-specific transcriptional regulation of the human gamma- and beta-globin genes and located inside or in the immediate proximity of these genes have been identified as sequences which bind erythroid-specific factors. In the present study, we found two regions located within 1 kb in 5' to the alpha 2- and in 3' to the alpha 1-globin genes which contribute to the induction of human alpha-globin genes following erythroid differentiation in stable MEL transformants. By DNAse I footprinting and gel mobility shift assays, we identified several GATA-1 and one AP-1/NF-E2-binding sites located inside these regions. These results strengthen the idea that, like for all other globin genes, flanking regions contribute in vivo to the regulation of human alpha-globin gene expression.
Gourdon
G
G
Centre de Génétique Moléculaire et Cellulaire, CNRS UMR 106, Villeurbanne, France.
Morlé
F
F
Roche
J
J
Tourneur
N
N
Joulain
V
V
Godet
J
J
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D016172:DNA Fingerprinting; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000235:genetics; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D015870:Gene Expression; D015973:Gene Expression Regulation, Leukemic; D005914:Globins / Q000235:genetics*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D008969:Molecular Sequence Data; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; D009841:Oligonucleotides / Q000032:analysis; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; C494692:NFE2 protein, human; D009841:Oligonucleotides; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D005914:Globins; D003850:Deoxyribonuclease I