Differential binding of (+) and (-) gossypol to plasma protein and their entry into rat testis.
Concentrations of (+) and (-) gossypol were measured by high performance liquid chromatography after they were incubated with plasma proteins in vitro. The concentration of (-) gossypol decreased more than the concentration of (+) gossypol. A similar decrease in free gossypol concentrations in the blood plasma of rats was observed after intravenous infusion of gossypol enantiomers. The concentration of (-) gossypol was also found to be lower than the concentration of (+) gossypol at the blood-testis barrier. The biological effect of (-) gossypol probably results from its stereospecific binding to extra- and intracellular proteins in vivo and inhibition of the biological activity of some proteins.
Wang
J M
JM
Department of Pharmacology, Nanjing Institute of Materia Medica, People's Republic of China.
Tao
L
L
Wu
X L
XL
Lin
L X
LX
Wu
J
J
Wang
M
M
Zhang
G Y
GY
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006072:Gossypol / Q000378:metabolism*; D007536:Isomerism; D008297:Male; D011485:Protein Binding / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000378:metabolism*
D001798:Blood Proteins; D006072:Gossypol