Adaptations of Goldner's Masson trichrome stain for the study of undecalcified plastic embedded bone.
Specialized adaptations for application of Goldner's Masson trichrome stain to plastic embedded undecalcified bone specimens are presented. This stain can be used successfully on methyl-glycol methacrylate, glycol methacrylate and Spurr embedded bones. The stain affords the advantage of good cellular staining due to the hematoxylin component with concomitant sharp discrimination of mature bone matrix which stains green, immature new bone matrix which stains red, and calcified cartilage which stains very pale green. Use of red filters during photomicrography aids in bone-osteoid discrimination in black and white photographs.
Gruber
H E
HE
Medical Genetics-Birth Defects Center, Cedars-Sinai Medical Center, Los Angeles, California 90048.
D001391:Azo Compounds; D001706:Biopsy; D001851:Bone Diseases, Metabolic / Q000473:pathology*; D001842:Bone and Bones / Q000473:pathology*; D002113:Calcification, Physiologic / Q000502:physiology; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D006801:Humans; D007231:Infant, Newborn; D008689:Methacrylates; D008739:Methyl Green; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D010024:Osteoporosis / Q000473:pathology; D016613:Plastic Embedding / Q000379:methods
D001391:Azo Compounds; D004396:Coloring Agents; D008689:Methacrylates; D008768:Methylmethacrylates; C017768:trichrome stain; D020366:Methylmethacrylate; C005044:hydroxyethyl methacrylate; D008739:Methyl Green; D004801:Eosine Yellowish-(YS)