Cloturin: effect on energy-producing processes in Ehrlich ascites and P388 murine leukaemia cells.
The main purpose of the present investigation was to study the effect of cloturin on aerobic glycolysis, endogenous and exogenous respiration and the level of ATP in both Ehrlich ascites carcinoma (EAC) and P388 murine leukaemia cells incubated in vitro. Also its effect on the level of total (T-SH) and non-protein (NP-SH) thiol groups was investigated. A significant inhibition of aerobic glycolysis was found only in P388 cells after 60 min of cloturin action. Cloturin inhibited both endogenous and exogenous respiration of EAC with succinate as substrate. Cloturin decreased the level of ATP after 2 h incubation in both types of tumour cell. The level of NP-SH was decreased more than that of T-SH in both types of cell.
Miko
M
M
Department of Microbiology, Slovak Technical University, Bratislava, Czechoslovakia.
Krepelka
J
J
Melka
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D007941:Leukemia P388 / Q000378:metabolism*; D015122:Mercaptopurine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D009150:Mustard Compounds / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D009150:Mustard Compounds; D013438:Sulfhydryl Compounds; C057648:Cloturin; D000255:Adenosine Triphosphate; D015122:Mercaptopurine; D005947:Glucose