A rapid method for preparation of bacterial chromosomal DNA in agarose plugs using Thermus Rt41A proteinase.
Borges
K M
KM
Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
Bergquist
P L
PL
D001426:Bacterial Proteins / Q000378:metabolism*; D002876:Chromosomes, Bacterial / Q000737:chemistry*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D016521:Electrophoresis, Gel, Pulsed-Field; D019286:Endopeptidase K; D012685:Sepharose; D012697:Serine Endopeptidases / Q000378:metabolism*; D013696:Temperature; D013824:Thermus / Q000201:enzymology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012685:Sepharose; D012697:Serine Endopeptidases; C075486:Thermus aquaticus Rt41A proteinase; D019286:Endopeptidase K