Mouse recombinant interferon-beta augments the induction of macrophage cytolytic activity by interleukin-2 in vitro.
We investigated the augmenting effect of mouse recombinant interferon-beta (MurIFN-beta) on the induction of macrophage (M phi) cytolytic activity by interleukin-2 (IL-2). C3H/He spleen cells were cultured with various doses of recombinant (r) IL-2 and/or MurIFN-beta for 4 days, and their cytotoxic activities against syngeneic M phi were assessed by a 4-h 51Cr-release assay. Although MurIFN-beta itself induced little M phi cytolytic activity, it significantly augmented the IL-2-induced cytotoxicity against M phi. MurIFN-beta showed a dose-dependent enhancement on the induction of anti-M phi activity by IL-2 with a peak at 2,000-10,000 U/ml, and the augmenting effect of rIFN-beta was more marked on lower doses of rIL-2 than on higher doses. This augmenting effect was also observed in IFN-alpha, whereas IFN-gamma showed no influence on IL-2-induced anti-M phi activity. The phenotype analysis by depletion technique with antibody and completement showed that anti-M phi effectors induced by either rIL-2 or the combination of rIL-2 and rIFN-beta were Thy-1+, asialoGM1+, L3T4-, and Ly2-. These results suggest that IFN-alpha or IFN-beta has a synergistic effect with IL-2 on the induction of nonspecific killer cells with M phi cytolytic activity.
Chen
M F
MF
First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, Japan.
Suzuki
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003165:Complement System Proteins / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004357:Drug Synergism; D005260:Female; D007370:Interferon Type I / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007370:Interferon Type I; D007376:Interleukin-2; D011994:Recombinant Proteins; D003165:Complement System Proteins