Lipid microsphere containing lipophilic heme: preparation and oxygen transportation under physiological conditions.
Lipophilic heme (1-laurylimidazole-ligated 5,10,15,20-tetrakis(alpha, alpha, alpha, alpha-o- pivalamidophenyl)porphinatoiron(II) complex) is solubilized in lipid (triglyceride) at high concentrations and emulsified with a phospholipid in physiological salt solution, giving a deeply red-colored suspension of lipid microspheres (approx. 250 nm in diameter). The heme forms an oxygen adduct in a similar manner as oxyhemoglobin and the lipid microspheres take up and release oxygen reversibly at 37 degrees C in the aqueous medium. The oxygen-transporting ability is comparable with that of the red blood cell. Intravenous injection of the heme/lipid microsphere solution to rabbits demonstrates that it transports oxygen even in vivo and that it is cleared from the blood stream with a half-life time of approx. 1 h.
Tsuchida
E
E
Department of Polymer Chemistry, Waseda University, Tokyo, Japan.
Nishide
H
H
Komatsu
T
T
Yamamoto
K
K
Matsubuchi
E
E
Kobayashi
K
K
D000818:Animals; D004337:Drug Carriers; D006207:Half-Life; D006418:Heme / Q000032:analysis; D008055:Lipids; D008863:Microspheres; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits
D004337:Drug Carriers; D008055:Lipids; D006418:Heme; D010100:Oxygen