Production of enterotoxin and cytotoxin in Campylobacter jejuni strains isolated in Costa Rica.
The production of toxins by 79 strains of Campylobacter jejuni isolated in Costa Rica from children with campylobacter-induced diarrhoea (44 strains) and from chickens (35 strains) was studied. An enterotoxic effect giving a rounding of mouse adrenocortical tumour (Y1) cells, which could be neutralised with antitoxin against Escherichia coli heat-labile enterotoxin, was detected in supernates from 16 (62%) of 26 strains from children with watery diarrhoea, in 5 (28%) of 18 strains from children with bloody or inflammatory diarrhoea, and in 12 (34%) of the 35 strains from chickens. Cytotoxic effects in human lung fibroblasts (MRC-5), African Green monkey kidney (Vero) cells and human cervical carcinoma (HeLa) cells were observed in none of the 26 strains from children with watery diarrhoea, in 2 (11%) of the 18 strains from children with bloody or inflammatory diarrhoea, and in 6 (17%) of the 35 strains from chickens. The simultaneous production of enterotoxin and cytotoxin was detected in four strains. The cytotoxic effect, which was most prominent in cells freshly seeded at a low density, appeared as a lethal rounding of the cells. Fibroblasts were more sensitive than epithelial cells. The effects of the supernates were inactivated by heating at 100 degrees C for 10 min and decreased after 1 week at 4 degrees C. The production of toxins was lost after storage of the strains for one year at -70 degrees C.
Florin
I
I
Department of Bacteriology, Karolinska Institute, Stockholm, Sweden.
Antillon
F
F
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis; D002169:Campylobacter Infections / Q000382:microbiology* / Q000662:veterinary; D016123:Campylobacter jejuni / Q000378:metabolism*; D002460:Cell Line; D002645:Chickens; D003364:Costa Rica; D003603:Cytotoxins / Q000096:biosynthesis*; D003968:Diarrhea, Infantile / Q000382:microbiology*; D004768:Enterotoxins / Q000096:biosynthesis*; D004848:Epithelium / Q000382:microbiology; D029968:Escherichia coli Proteins; D005347:Fibroblasts / Q000382:microbiology; D006367:HeLa Cells; D006801:Humans; D007223:Infant; D011201:Poultry Diseases / Q000382:microbiology; D014709:Vero Cells
D001427:Bacterial Toxins; D003603:Cytotoxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli