Tissue sulfate determination by ion chromatography.
The application of controlled-flow anion chromatography to assay inorganic sulfate in biological fluids and tissues is described. The eluent used in previous methods for analyzing sulfate in biological fluids has been modified by adding 4.5% acetonitrile to separate sulfate from a co-eluting peak. To markedly increase the life of the column, the tissue samples were further diluted, extracted with chloroform, and analyzed at a lower detection range (0.3 microS). The method has been shown to be applicable for determining sulfate in tissues as well as biological fluids.
Rozman
P
P
Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City 66103.
Kim
H J
HJ
Madhu
C
C
Klaassen
C D
CD
D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D002853:Chromatography, Liquid; D007477:Ions; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000378:metabolism*
D007477:Ions; D013431:Sulfates