Fluorescent location of abnormal cells in cell smears obtained from the lungs of patients with lung cancer.
A bronchoscope fitted with a brush was used to collect cells from patients with lung tumours. The cells were either made into smears or deposited as monolayers on microscope slides. Using a fluorescent probe for the cell surface enzyme, referred to as guanidinobenzoatase, abnormal cells were readily distinguished from normal cells which lacked this enzyme.
Steven
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FS
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, UK.
Lam
S
S
Macaulay
C
C
Palcic
B
B
D000585:Aminacrine; D015415:Biomarkers; D001999:Bronchoscopy; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D010450:Endopeptidases; D005456:Fluorescent Dyes; D006652:Histological Techniques; D006801:Humans; D008168:Lung / Q000201:enzymology / Q000473:pathology*; D008175:Lung Neoplasms / Q000201:enzymology / Q000473:pathology*; D008856:Microscopy, Fluorescence
D015415:Biomarkers; D005456:Fluorescent Dyes; D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases