Measurement of the zeta potential of human platelets by the use of laser-light scattering.
An instrument was developed to detect the shift in scattering of laser light that occurs when particles in suspension move in a chamber with an electrical load. The instrument measures the zeta potential of particles. We applied the instrument to study human blood cells. Platelet-rich plasma was used because of the stability of the suspension, without the sedimentation or autoaggregation that is often seen with red or white blood cells. The reproducibility of the measurements was satisfactory when there were enough platelets in the suspension. Platelets from healthy controls (n = 136) had a potential of -14.20 +/- 1.64 mV at the detection angle of 17.1 degrees. Platelets from patients with essential thrombocytosis (n = 16) or polycythemia vera (n = 8) had higher potentials than the healthy controls.
Tatsumi
N
N
Department of Clinical and Laboratory Medicine, Osaka City University Medical School.
Tsuda
I
I
Masaoka
M
M
Imai
K
K
D001792:Blood Platelets / Q000378:metabolism*; D006801:Humans; D007834:Lasers; D008027:Light; D008564:Membrane Potentials; D011087:Polycythemia Vera / Q000378:metabolism; D012542:Scattering, Radiation; D013922:Thrombocytosis / Q000378:metabolism