Establishment and characterization of a mouse Schwann cell line which produces myelin in vivo.
A Schwann cell line (MSC 80) was established from purified mouse Schwann cell cultures using large doses of serum. MSC 80 cell line is an aneuploid cell line which has a doubling time of 17 hr and has been maintained through more than 110 passages. Most of MSC 80 cells are of bipolar or stellate (3-5 processes) shape. A few others are irregular in shape, gigantic, and multinucleated. All MSC 80 cells express antigens of myelin-forming Schwann cells such as S-100, 224/58, laminin, and other glycoproteins of the extracellular matrix. However, they also express the non-myelin-forming Schwann cell antigen GFAP. By time-lapse cinematography, MSC 80 cells exhibit the Schwann cell characteristic rhythmical undulations. When induced to form aggregates in agar, they form intercellular junctions and basement membrane-like structures. In addition, after transplantation in or at a distance from a lysolecithin induced lesion, MSC 80 cells form myelin around the host demyelinated axons. MSC 80 cells thus express, when isolated in vitro, some of the normal myelin-forming Schwann cell phenotype. In addition, they present the major advantage of forming myelin when associated with axons in vivo.
Boutry
J M
JM
Laboratoire de Neuropathologie R. Escourolle, FRA Association Claude Bernard, Hôpital de la Salpêtrière, Paris, France.
Hauw
J J
JJ
Gansmüller
A
A
Di-Bert
N
N
Pouchelet
M
M
Baron-Van Evercooren
A
A
D000818:Animals; D001562:Benzimidazoles; D015415:Biomarkers; D002460:Cell Line; D002465:Cell Movement; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D051379:Mice; D009186:Myelin Sheath / Q000378:metabolism*; D010641:Phenotype; D009418:S100 Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000166:cytology
D001562:Benzimidazoles; D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins; C017807:bisbenzimide ethoxide trihydrochloride