Anaerobic toluene oxidation to benzyl alcohol and benzaldehyde in a denitrifying Pseudomonas strain.
The denitrifying Pseudomonas strain K172 was grown with a generation time of 6 h to a cell density of 0.4 g (dry weight) per liter with toluene and nitrate as substrates. We found that anaerobic cell suspensions oxidize [14C]toluene first to [14C]benzyl alcohol and subsequently to [14C]benzaldehyde. This proves that the methyl group of toluene is oxidized without molecular oxygen to a hydroxymethyl group.
Altenschmidt
U
U
Angewandte Mikrobiologie, University of Ulm, Germany.
Fuchs
G
G
D000693:Anaerobiosis; D001547:Benzaldehydes / Q000378:metabolism*; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*
D001547:Benzaldehydes; D001592:Benzyl Alcohols; D009566:Nitrates; D014050:Toluene; D019905:Benzyl Alcohol; C032175:benzaldehyde