title: Complete structure of eclosion hormone of Manduca sexta. Assignment of disulfide bond location.
abstract: The locations of the three disulfide bonds of eclosion hormone (EH) isolated from Manduca sexta were assigned by sequence analysis of thermolysin fragments and by comparison of a key heterodimeric fragment to regiospecifically synthesized parallel and antiparallel isomers. We elucidated the complete structure of Manduca EH as a 62-residue peptide which has three disulfide bonds between Cys14-Cys38, Cys18-Cys34, and Cys21-Cys49.
authors.affiliation: Sandoz Crop Protection Corporation, Palo Alto, CA.
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: J P
authors.initials: JP
authors.lastname: Li
authors.forename: A S
authors.initials: AS
authors.lastname: Lui
authors.forename: S J
authors.initials: SJ
authors.lastname: Kramer
authors.forename: D A
authors.initials: DA
authors.lastname: Schooley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000737:chemistry; D007301:Insect Hormones / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry*; D011487:Protein Conformation; D013820:Thermolysin / Q000378:metabolism
chemical_list: D004220:Disulfides; D007301:Insect Hormones; C042645:eclosion hormone; D013820:Thermolysin