title: Sequence and variability of the 5.8S and 26S rRNA genes of Pneumocystis carinii.
abstract: The sequence of the coding region of the rRNA operon of rat-derived Pneumocystis carinii has been completed, including the genes for 5.8S and 26S rRNA. These genes show homology to the rRNA genes of yeast, and an apparent group I self-splicing intron is present in the 26S rRNA gene. Like a similar intron in the 16S rRNA gene, this intron is in a phylogenetically conserved region. Variation in the 26S rRNA sequence was noted between P. carinii organisms isolated from two different sources.
authors.affiliation: Department of Molecular Genetics and Microbiology, UMDNJ--Robert Wood Johnson Medical School, Piscataway 08854-5635.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Rocourt
authors.forename: S
authors.initials: S
authors.lastname: Pan
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: M J
authors.initials: MJ
authors.lastname: Leibowitz
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D005800:Genes, Fungal; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon / Q000235:genetics; D011010:Pneumocystis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D016337:RNA, Catalytic / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D016337:RNA, Catalytic; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S; C065096:RNA, ribosomal, 26S