A method for the determination of sex hormone binding globulin using Concanavalin A-sepharose.
A binding assay for sex hormone binding globulin (SHBG) has been developed in which SHBG is saturated with tritiated dihydrotestosterone (DHT). Separation of bound and free DHT is achieved by using Concanavalin A-Sepharose as a solid phase matrix. The method is described and its performance, including linearity, imprecision and comparison with other methods, is assessed. The assay is simple and robust and is suitable for analysis of samples of plasma or serum for clinical or research use.
Whittaker
J A
JA
Department of Chemical Pathology and Immunology, School of Medicine, University of Leeds, UK.
Cawood
M L
ML
Oakey
R E
RE
D013196:Dihydrotestosterone / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D012685:Sepharose / Q000031:analogs & derivatives; D012738:Sex Hormone-Binding Globulin / Q000032:analysis*
D012738:Sex Hormone-Binding Globulin; C028326:concanavalin A-sepharose; D013196:Dihydrotestosterone; D012685:Sepharose