Structural changes in condylar cartilage following prolonged exposure to the human parathyroid hormone fragment (hPTH) 1-34 in vitro.
This investigation presents the structural changes in condylar cartilage incubated in the presence of human parathyroid hormone (1-34) in an organ culture system for 6 to 12 days. Control cultures maintained their cartilaginous characteristics whereas human parathyroid hormone (1-34)-treated cultures revealed the following modifications: (1) The chondroprogenitor cell zone at the apical region of the explant underwent a substantial enlargement. The cells changed from a mesenchyme-like morphology into polygonal, glycogen-rich cells that were tightly attached to each other by a fibrillar intercellular matrix, but even by 12 days the apical region was comprised of healthy cells. (2) The mineralizing zone in the hypertrophic cartilage revealed a change in its cellular population. Hypertrophic chondrocytes were replaced by cells with amoeboid extensions and large numbers of secretory granules or vesicles. Based upon the above findings it appears that the chondroprogenitor cells that are initially stimulated to proliferate, are being suppressed from subsequent differentiation into chondroblasts; and that hypertrophic chondrocytes apparently undergo a dedifferentiation process followed by development into an as yet unknown cell population.
Lewinson
D
D
Laboratory for Musculoskeletal Research, Rappaport Family Institute for Research in the Medical Sciences, Haifa, Israel.
Shurtz-Swirski
R
R
Shenzer
P
P
Wingender
E
E
Mayer
H
H
Silbermann
M
M
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D001861:Bone Regeneration; D002358:Cartilage, Articular / Q000187:drug effects* / Q000648:ultrastructure; D002454:Cell Differentiation; D008335:Mandibular Condyle / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D009924:Organ Culture Techniques; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D019379:Teriparatide
D010281:Parathyroid Hormone; D010446:Peptide Fragments; D019379:Teriparatide