Quantitative liquid chromatography, thermospray/tandem mass spectrometry (LC/TSP/MS/MS) analysis of some thermolabile benzodiazepines in whole-blood.
An LC/TSP/MS/MS selected reaction monitoring (SRM) method in the daughter ion scan mode can be applied successfully for the quantitative determination of thermolabile benzodiazepines in whole-blood following an Extrelut extraction method. Detection limits as low as 100 pg per injection can be reached for all benzodiazepines. The benzodiazepines under investigation proved to be stable, with the exception of ketazolam for which complete degradation to diazepam was observed.
Verweij
A M
AM
Department of General Chemistry, Ministry of Justice, Rijswijk, The Netherlands.
Lipman
P J
PJ
Zweipfenning
P G
PG
D001569:Benzodiazepines / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006358:Hot Temperature; D006801:Humans; D013058:Mass Spectrometry; D015394:Molecular Structure; D009330:Nebulizers and Vaporizers; D012015:Reference Standards; D012680:Sensitivity and Specificity
D001569:Benzodiazepines