A cell surface antigen associated with meiosis in male Staurdoderus scalaris (Orthopteran).
Immunofluorescence has identified seven monoclonal antibodies reactive with the surface of meiotic cells and absent in premeiotic cells. Analysis by immunogold electron microscopy indicated that these antigens were present on the external surface of the cells and were coincident with the presence of synaptonemal complexes in the nucleus. On immunoblots a common glycosylated protein of 205 kDa was recognized, in addition to smaller subunits, suggesting the presence of a protein complex comprised of smaller peptides.
Percy
M J
MJ
Division of Genetic Engineering, School of Biology and Biochemistry, Queen's University of Belfast.
Fletcher
H L
HL
Trudgett
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis*; D015415:Biomarkers; D005455:Fluorescent Antibody Technique; D006110:Grasshoppers / Q000276:immunology* / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D008540:Meiosis; D016253:Microscopy, Immunoelectron; D013094:Spermatozoa / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015415:Biomarkers