One-step preparation of Hageman factor fragment concentrate.
The Hageman factor fragment (HFf) concentrate was prepared from acetone and dextran sulphate activated human plasma by chromatography on a CM-Sephadex column. The preparation was free of the main kallikrein inhibitors--Cl-inactivator, alpha 2-macroglobulin, antithrombin III and alpha 1-antitrypsin. The HFf concentrate can serve as an activator of prekallikrein in patient plasma.
Simonianová
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Institute of Haematology and Blood Transfusion, Prague, Czechoslovakia.
D001769:Blood; D002853:Chromatography, Liquid; D004789:Enzyme Activation; D005174:Factor XII / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification; D011288:Prekallikrein / Q000378:metabolism
D010446:Peptide Fragments; D005174:Factor XII; D011288:Prekallikrein