Modulation by the insecticides heptachlor and chlordane of the cell-mediated immune proliferative responses of rhesus monkeys.
Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood samples from twelve young, healthy male rhesus monkeys. Triplicate cultures were made in RPMI 1640 with 10% heat-inactivated homologous plasma with or without the test mitogens (phytohemagglutinin, concanavalin A and pokeweed mitogen). Under a defined assay condition, although a different monkey responded to a different degree (3 to 4-fold difference in stimulation index) to a specific mitogen, the proliferative responses of all monkeys were affected significantly by the addition of the chlorinated hydrocarbon heptachlor or chlordane to the test cultures. The insecticides, at 10-40 microM, may also act as mitogens for the unstimulated monkey PBMC or stimulate the release of IL-2 from a mitogen-stimulated culture. At 80 microM, both heptachlor and chlordane completely suppressed the proliferation and IL-2 release of the monkey lymphocytes. These studies suggest immunomodulatory effects of the insecticides heptachlor and chlordane.
Chuang
L F
LF
Department of Medical Pharmacology and Toxicology, University of California, Davis 95616.
Liu
Y
Y
Killam
K
K
Chuang
R Y
RY
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002706:Chlordan / Q000494:pharmacology*; D003864:Depression, Chemical; D004347:Drug Interactions; D006533:Heptachlor / Q000494:pharmacology*; D007111:Immunity, Cellular / Q000187:drug effects*; D007376:Interleukin-2 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008253:Macaca mulatta / Q000276:immunology; D008297:Male; D008934:Mitogens
D007376:Interleukin-2; D008934:Mitogens; D002706:Chlordan; D006533:Heptachlor