Rapid and simple gas chromatographic measurement of lactic acid in red blood cells, plasma, and tumor cells after hyperthermia.
The content of lactic acid in red blood cells, plasma, and Ehrlich ascites tumor cells were measured by a gas-liquid chromatography using a column with a terephtalic acid support coated with polyethylene glycol-6000. The lactic acid contents were directly determined in aqueous samples, because they were converted to a volatile derivative in the column. The method was rapid and simple, compared with previous methods which need time-consuming conversion of lactic acid to volatile derivatives. Our measurements showed the increase in the contents of intra- and extracellular lactic acid after hyperthermia.
Kageyama
K
K
Radioisotope Centre, Osaka, Japan.
Onoyama
Y
Y
Nakajima
T
T
Tumura
M
M
Nishida
T
T
Kogawa
H
H
Tanabe
K
K
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism* / Q000473:pathology; D002849:Chromatography, Gas / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D006979:Hyperthermia, Induced; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D014407:Tumor Cells, Cultured
D007773:Lactates; D019344:Lactic Acid