A pre-embedding reaction method for localizing NADH reductase and succinic dehydrogenase in skeletal muscle.
Paraffin wax embedding methods suitable for demonstrating the distribution of enzyme activity in tissues sections are uncommon; most procedures rely on the use of frozen section techniques. This paper describes a system for demonstrating certain enzymes which involves incubation of the tissue with appropriate substrates before a Paramat wax embedding procedure. While it has distinct merits of its own, the procedure is eminently suitable for use where a cryostat is not available; it can also be readily applied to other enzymes and tissues.
Hawcroft
D
D
Department of Pharmacy, Leicester Polytechnic, United Kingdom.
Ball
M
M
Burrows
J
J
D000818:Animals; D009132:Muscles / Q000201:enzymology*; D009246:NADH Tetrazolium Reductase / Q000032:analysis*; D016612:Paraffin Embedding / Q000379:methods*; D051381:Rats; D013385:Succinate Dehydrogenase / Q000032:analysis*; D016707:Tissue Fixation
D013385:Succinate Dehydrogenase; D009246:NADH Tetrazolium Reductase