T-cell-specific membrane antigens in the Mexican axolotl (urodele amphibian).
Comparative analysis of SDS-PAGE patterns of axolotl spleen cells membrane detergent lysates showed important discrepancies between control and thymectomized animals. Among these, a 38-kD protein band, which appeared as a major protein in controls, was not or poorly expressed after thymectomy. A rabbit antiserum (L12) raised against the 38-kD eluted band labeled in indirect immunofluorescence 80-86% of thymocytes and 40-46% of mIg- lymphoid cells in the spleen. The anti-38-kD antibodies stained in Western blotting two antigenically related polypeptides of 38- and 36-kD on splenocyte membrane lysates. Two-dimensional NEPHGE-PAGE analysis indicated that the anti-38-kD antibodies reacted in the spleen with several gathered spots in the 7.8-8.2 pI range, corresponding to 38-36-kD microheterogeneous polypeptides. Most of these spots are not further expressed in thymectomized animals. These results support evidence that the 38-kD surface antigens can be considered as specific surface markers of the axolotl thymus-derived lymphocytes.
Kerfourn
F
F
Laboratoire d'Immunologie Comparée, Université Pierre et Marie Curie, CNRS URA 1135, Paris, France.
Guillet
F
F
Charlemagne
J
J
Tournefier
A
A
D000558:Ambystoma mexicanum / Q000276:immunology*; D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D008214:Lymphocytes / Q000276:immunology*; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D008970:Molecular Weight; D013934:Thymectomy
D000906:Antibodies; D000954:Antigens, Surface; D008565:Membrane Proteins