The combination of normal phase with reversed phase high performance liquid chromatography for the analysis of asparagine-linked neutral oligosaccharides labelled with p-aminobenzoic ethyl ester.
We have developed a novel approach for the analysis of asparagine-linked neutral oligosaccharides derived from glycoproteins. The oligosaccharides are labelled with p-aminobenzoic ethyl ester and the derivatives are separated on two high performance liquid chromatographic columns, one containing amide-silica and the other containing octadecyl-silica. The elution positions of 39 different ABEE-oligosaccharides on the two columns were plotted on a two-dimensional map. Unique non-overlapping positions of these oligosaccharides demonstrate that this technology would be useful for the identification of Asn-linked oligosaccharides at high sensitivity.
Matsuura
F
F
Department of Biotechnology, Faculty of Engineering, Fukuyama University, Hiroshima, Japan.
Ohta
M
M
Murakami
K
K
Hirano
K
K
Sweeley
C C
CC
D001216:Asparagine / Q000032:analysis*; D001566:Benzocaine / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D009844:Oligosaccharides / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D009844:Oligosaccharides; D001216:Asparagine; D001566:Benzocaine