Mobilization of recombinant plasmids from Staphylococcus aureus into coagulase negative Staphylococcus species.
pC221, a small nonconjugative staphylococcal plasmid, can be mobilized between staphylococci by pG01, a larger conjugative plasmid. pC221 carries the two transacting genes, mobA and mobB, which are needed for its mobilization. The products of these genes create a site-specific single-stranded nick (mobA) and then facilitate DNA transfer (mobB). Several useful Escherichia coli-staphylococcal shuttle plasmids containing the cloned single-stranded nick site were created and successfully mobilized into Staphylococcus aureus and two coagulase-negative staphylococci, S. epidermidis and S. saprophyticus, by providing mob genes (pC221) and conjugative transfer genes (pG01) in trans in the donor. These vectors may offer a genetic system for the introduction of recombinant plasmids into coagulase negative staphylococci.
Thomas
W D
WD
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison 53706.
Archer
G L
GL
D001426:Bacterial Proteins; D003030:Coagulase / Q000378:metabolism; D003227:Conjugation, Genetic / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000201:enzymology / Q000235:genetics; D015534:Trans-Activators
D001426:Bacterial Proteins; D003030:Coagulase; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; C072871:RSF1010 MobB protein, Staphylococcus; D015534:Trans-Activators