Phospholipid polar heads affect the generation of oxygen active species by Fe2+ autoxidation.
The possibility that phospholipid polar heads may influence Fe2+ reaction with molecular oxygen and, thus, the generation of oxygen active species was investigated. Dipalmitoyl phosphatidylcholine (DPPC) and DPPC/dipalmitoyl phosphatidic acid (DPPA) were utilized as model liposomes. Fe2+ oxidation, oxygen consumption, nitro blue tetrazolium reduction and 2-deoxyribose degradation were the parameters evaluated. Comparison of the results obtained clearly shows that the two types of polar heads differently affect iron chemistry. DPPC liposomes are ineffective. By contrast, Fe2+ oxidation by oxygen occurs in the presence of DPPC/DPPA liposomes. During this reaction, species able to reduce nitro blue tetrazolium and to degrade 2-deoxyribose are generated. The results obtained indicate that the polar heads of phospholipids, by influencing Fe2+ autoxidation, generate dangerous oxygen species which may exert an active role in the oxidation of the associated hydrophobic components of the phospholipids.
Tadolini
B
B
Dipartimento di Biochimica, Università di Bologna, Italy.
Motta
P
P
Sechi
A M
AM
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D003855:Deoxyribose; D005609:Free Radicals; D007501:Iron / Q000737:chemistry*; D015227:Lipid Peroxidation; D008081:Liposomes; D015394:Molecular Structure; D009580:Nitroblue Tetrazolium; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D010712:Phosphatidic Acids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*
D005609:Free Radicals; D008081:Liposomes; D010712:Phosphatidic Acids; D010743:Phospholipids; C031599:dipalmitoylphosphatidic acid; D015060:1,2-Dipalmitoylphosphatidylcholine; D009580:Nitroblue Tetrazolium; D003855:Deoxyribose; D007501:Iron; D010100:Oxygen