High resolution of multiple forms of red blood cell enzymes using a Toyopearl DEAE 650 S.
We have investigated a new anion exchange chromatographic support (Toyopearl DEAE 650 S) which simultaneously allows easily to remove hemoglobin from hemolysates and to obtain a very high resolution of enzymes present in multiple forms. The results obtained are better than those obtainable using an anion-exchange HPLC column. The data obtained at analytical level suggest a wider use of this new matrix also for preparative purposes without significant changes in the resolution.
Stocchi
V
V
Istituto di Chimica Biologica, Giorgio Fornaini, Università degli Studi, Urbino, Italy.
Masat
L
L
Biagiarelli
B
B
Accorsi
A
A
Piccoli
G
G
Palma
F
F
Cucchiarini
L
L
Dachà
M
M
D000818:Animals; D000837:Anion Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology*; D004983:Ethanolamines; D006593:Hexokinase / Q000302:isolation & purification; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification*; D011108:Polymers; D011683:Purine-Nucleoside Phosphorylase / Q000302:isolation & purification; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology; D013552:Swine
D000837:Anion Exchange Resins; C064803:DEAE-Toyopearl 650S; D004983:Ethanolamines; D007527:Isoenzymes; D011108:Polymers; D011683:Purine-Nucleoside Phosphorylase; D006593:Hexokinase