Transformation of the gram-positive bacterium Clavibacter xyli subsp. cynodontis by electroporation with plasmids from the IncP incompatibility group.
We report the transformation of a gram-positive bacterium, Clavibacter xyli subsp. cynodontis, with several plasmids in the IncP incompatibility group from gram-negative bacteria. Our results suggest that IncP plasmids may be transferable to other gram-positive organisms. After optimizing electroporation parameters, we obtained a maximum of 2 x 10(5) transformants per microgram of DNA. The availability of a transformation system for this bacteria will facilitate its use in indirectly expressing beneficial traits in plants.
Metzler
M C
MC
Department of Plant Pathology, Cook College, Rutgers University, New Brunswick, New Jersey 08903.
Zhang
Y P
YP
Chen
T A
TA
D000192:Actinomycetales / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004558:Electric Stimulation; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids; D014169:Transformation, Bacterial
D004269:DNA, Bacterial