Complete structure of eclosion hormone of Manduca sexta. Assignment of disulfide bond location.
The locations of the three disulfide bonds of eclosion hormone (EH) isolated from Manduca sexta were assigned by sequence analysis of thermolysin fragments and by comparison of a key heterodimeric fragment to regiospecifically synthesized parallel and antiparallel isomers. We elucidated the complete structure of Manduca EH as a 62-residue peptide which has three disulfide bonds between Cys14-Cys38, Cys18-Cys34, and Cys21-Cys49.
Kataoka
H
H
Sandoz Crop Protection Corporation, Palo Alto, CA.
Li
J P
JP
Lui
A S
AS
Kramer
S J
SJ
Schooley
D A
DA
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000737:chemistry; D007301:Insect Hormones / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry*; D011487:Protein Conformation; D013820:Thermolysin / Q000378:metabolism
D004220:Disulfides; D007301:Insect Hormones; C042645:eclosion hormone; D013820:Thermolysin