Analysis of sterol esters from alga and yeast by high-performance liquid chromatography and capillary gas chromatography-mass spectrometry with chemical ionization.
Sterol esters from the green alga Chlorella kessleri and the yeast Saccharomyces cerevisiae were analysed by tandem high-performance liquid chromatography (HPLC-HPLC). Non-polar lipids were separated by normal-phase HPLC into individual classes and the fraction of sterol esters was subsequently separated by reversed-phase HPLC into intact molecular species. Further separation and identification of the fractions after HPLC were effected by capillary gas chromatography-mass spectrometry (cGC-MS) with positive and/or negative chemical ionization. A technique consisting of direct injection of the sample after its passage through the detector into the on-column injector of the gas chromatograph was used to transfer the sample from HPLC-HPLC to cGC-MS. By means of this method it was possible to demonstrate more than 30 sterols in both the alga and the yeast, and more than 20 new sterol esters were detected.
Rezanka
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Institute of Microbiology, Prague, Czechoslovakia.
D000460:Chlorophyta / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004952:Esters / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013261:Sterols / Q000032:analysis*
D004952:Esters; D005227:Fatty Acids; D013261:Sterols