Implication of glucocorticoid receptors in the stimulation of human glioma cell proliferation by dexamethasone.
Dexamethasone is frequently used in the therapy of brain tumor patients. We investigated the effect of dexamethasone on the proliferation of three short-term and four established human glioma cell lines in vitro, using a microculture tetrazolium assay to determine growth rates. In one short-term culture and in one established cell line dexamethasone consistently stimulated the proliferation in a concentration-dependent way. The proliferation was maximally enhanced at a concentration of approximately 0.1 microM. In these two cell lines a relatively high level of glucocorticoid receptors was present, whereas low levels of glucocorticoid receptors were found in the other cell lines. In addition, we demonstrated that the stimulatory effects of dexamethasone on the proliferation of the glioma cell lines can be antagonized by the antiglucocorticoid RU38486. The results demonstrate unequivocally that the glucocorticoid receptor plays a role in the growth stimulating effect of dexamethasone.
Langeveld
C H
CH
Department of Neurology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
van Waas
M P
MP
Stoof
J C
JC
Sutanto
W
W
de Kloet
E R
ER
Wolbers
J G
JG
Heimans
J J
JJ
D002455:Cell Division / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D011965:Receptors, Glucocorticoid; D013778:Tetrazolium Salts; D013844:Thiazoles; D015735:Mifepristone; D003907:Dexamethasone; C022616:thiazolyl blue