Binding of Salmonella strains to immobilized intestinal mucosal preparations from broiler chickens.
The binding kinetics of radiolabeled Salmonella california 1989/O (mannose-sensitive hemagglutinin-positive [MSHA+]) to immobilized mucus or enterocytes isolated from broiler ceca and inhibition of binding by D-mannose and sodium metaperiodate were characteristic of adherence of mannose-sensitive type 1 fimbriae of bacteria to eukaryotic mannose-containing receptors. Binding by radiolabeled strains 1989/O (in the presence of D-mannose) and S. typhimurium S 7471 N (MSHA-, non-fimbriated) indicated non-specific binding that was characterized by less binding to enterocytes and mucus and lack of inhibition by carbohydrates or prior treatment with sodium metaperiodate. Inhibition of non-specific binding to enterocytes by pretreatment with various enzymes or by the presence of tetramethylurea or p-nitrophenol (known to disrupt hydrophobic interactions) indicate involvement of multiple sites and hydrophobic bonding. Strain-specific outer-membrane preparations inhibited non-specific binding to a greater extent than did lipopolysaccharide, Escherichia coli outer-membrane preparations, or bovine serum albumin.
Craven
S E
SE
Poultry Microbiological Safety Research Unit, R. B. Russell Agricultural Research Center, Athens, Georgia 30613.
Cox
N A
NA
Bailey
J S
JS
Blankenship
L C
LC
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D001425:Bacterial Outer Membrane Proteins / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology; D002432:Cecum / Q000187:drug effects / Q000382:microbiology*; D002478:Cells, Cultured; D002645:Chickens; D046508:Culture Techniques; D010450:Endopeptidases / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000382:microbiology*; D008782:Methylurea Compounds / Q000494:pharmacology; D009596:Nitrophenols / Q000494:pharmacology; D012475:Salmonella / Q000187:drug effects / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D002241:Carbohydrates; D008782:Methylurea Compounds; D009596:Nitrophenols; C004168:1,1,3,3-tetramethylurea; D010450:Endopeptidases; C024836:4-nitrophenol