Effect of irradiation on the prostaglandin system of human hypernephroma cells.
Specific PGE1-, PGE2-, PGI2-binding sites and the eicosanoid production were investigated in cultured human hypernephroma cells. Low numbers of PGE1-, PGE2- as well as PGI2-high-affinity binding sites and high levels of PG-production were found as compared to normal kidney. Treatment with acetylsalicyclic acid (ASA) significantly (p less than 0.01) increased PGE1-, PGE2- and PGI2-binding sites and inhibited PG-formation. Following irradiation, PG-receptors and eicosanoid generation were significantly (p less than 0.01) diminished, however, were insignificantly lowered in the ASA-treated group. These findings suggest that ASA may modify the radioresponse of cultured hypernephroma cells by preventing the decrease of PG-receptors induced by irradiation.
Li
S R
SR
Department of Nuclear Medicine, University of Vienna, Austria.
Virgolini
I
I
Wandl
E
E
Yang
Q
Q
Sinzinger
H
H
D000368:Aged; D001241:Aspirin / Q000494:pharmacology; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011453:Prostaglandins / Q000378:metabolism*; D011982:Receptors, Prostaglandin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D014407:Tumor Cells, Cultured
D011453:Prostaglandins; D011982:Receptors, Prostaglandin; D001241:Aspirin