Regulation of major histocompatibility complex (MHC) products in fresh and cultured human fetal pancreata aged 9-16 gestational weeks by gamma-interferon.
Immunological data on the human fetal pancreas (HFP) are mainly confined to its constitutive expression of the MHC antigens. However, cytokines, such as gamma-interferon (g-IFN), released by lymphocytes during immune reactions, can induce or upregulate the expression of MHC products in allografts and alter their immunological behaviour. We investigated the effects of g-IFN on fresh and cultured HFPs aged 9-16 gestational weeks (gw). Following g-IFN stimulation of fresh HFPs, there was class I hyperexpression by the ductal cells, and some of the ductal, endothelial and islet cells also became class II+. Conventional tissue culture (5% CO2 in air at 37 degrees C) reduced the number of interstitial class II+ cells within the HFP after 1 week but was associated with de novo class I expression by some of the ductal cells. Remarkably, the changes in major histocompatibility complex (MHC) antigen expression by the ductal cells occurred earlier and were markedly enhanced when the HFPs were cultured beforehand. The number of interstitial class II+ cells in fresh and cultured HFPs was not influenced by g-IFN. The significance of these observations with regard to clinical HFP transplantation is discussed.
Koo Seen Lin
L C
LC
Renal Unit, Guy's Hospital, London, UK.
Welsh
K I
KI
Koffman
C G
CG
McColl
I
I
D046508:Culture Techniques; D016332:Fetal Tissue Transplantation / Q000276:immunology; D005333:Fetus / Q000276:immunology; D005865:Gestational Age; D006680:HLA Antigens / Q000378:metabolism*; D006681:HLA-D Antigens / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology*; D010179:Pancreas / Q000276:immunology*; D016035:Pancreas Transplantation / Q000276:immunology
D006680:HLA Antigens; D006681:HLA-D Antigens; D015395:Histocompatibility Antigens Class I; D007371:Interferon-gamma