title: Determination of panaxadiol and panaxatriol in ginseng and its preparations by capillary supercritical fluid chromatography (SFC).
abstract: Capillary supercritical fluid chromatographic (SFC) method has been developed for the determination of panaxadiol and panaxatriol in ginseng and its preparations. 0.1 g ginseng or an appropriate amount of its preparations was hydrolysed by 15% H2SO4 in an ethanol:water (1:1 v/v) solution for 4 h followed by 15% NaOH for 0.5 h. The mixture was extracted by cyclohexane. The cyclohexane extracts were purified by a partition column and concentrated by an adsorption column and then analysed by SFC. Methyltestosterone was used as the internal standard.
authors.affiliation: College of Pharmacy, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Li
authors.forename: X L
authors.initials: XL
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Hong
authors.forename: J Y
authors.initials: JY
authors.lastname: Liu
authors.forename: M Y
authors.initials: MY
authors.lastname: Zhang
mesh_terms: D002845:Chromatography / Q000379:methods*; D005410:Flame Ionization; D036145:Ginsenosides; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal; D012503:Saponins / Q000032:analysis*; D014315:Triterpenes / Q000032:analysis*
chemical_list: D036145:Ginsenosides; D012503:Saponins; D014315:Triterpenes; C004564:panaxadiol; C004563:panaxatriol