High-performance liquid chromatographic analysis of FCE 24304 (6-methylenandrosta-1,4-diene-3,17-dione) and FCE 24928 (4-aminoandrosta-1,4,6-triene-3,17-dione), two new aromatase inhibitors.
The cytochrome P-450-dependent aromatase enzyme plays an important role in hormone-dependent diseases. Many products that inhibit this type of enzyme were obtained: FCE 24304 (I) and FCE 24928 (II) proved to possess remarkable activity and are presently under development. Compounds I and II and their synthetic intermediates are analyzed by means of a high-performance liquid chromatographic method, affording rapid and efficient separation, good resolution and identification of all the examined compounds. The linearity, specificity, sensitivity, precision and accuracy for the method are also provided.
Del Nero
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S
Department of Analytical Chemistry, R & D Farmitalia Carlo Erba Srl, Milan, Italy.
Di Somma
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M
Vigevani
A
A
D000730:Androstadienes / Q000032:analysis* / Q000494:pharmacology; D000733:Androstatrienes / Q000032:analysis* / Q000494:pharmacology; D047072:Aromatase Inhibitors; D002851:Chromatography, High Pressure Liquid; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000730:Androstadienes; D000733:Androstatrienes; D047072:Aromatase Inhibitors; C075874:minamestane; C056516:exemestane