Cationic liposomes enhance targeted delivery and expression of exogenous DNA mediated by N-terminal modified poly(L-lysine)-antibody conjugate in mouse lung endothelial cells.
A new and improved system for targeted gene delivery and expression is described. Transfection efficiency of N-terminal modified poly(L-lysine) (NPLL) conjugated with anti-thrombomodulin antibody 34A can be improved by adding to the system a lipophilic component, cationic liposomes. DNA, antibody conjugate and cationic liposomes form a ternary electrostatic complex which preserves the ability to bind specifically to the target cells. At the same time the addition of liposomes enhance the specific transfection efficiency of antibody-polylysine/DNA binary complex by 10 to 20-fold in mouse lung endothelial cells in culture.
Trubetskoy
V S
VS
Department of Biochemistry, University of Tennessee, Knoxville.
Torchilin
V P
VP
Kennel
S
S
Huang
L
L
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D004337:Drug Carriers / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D051379:Mice; D011107:Polylysine / Q000378:metabolism*; D014162:Transfection
D000906:Antibodies; D004337:Drug Carriers; D008081:Liposomes; D011107:Polylysine; D004247:DNA