Ion-exchange high-performance liquid chromatography analysis of oligodeoxyribonucleotide phosphorothioates.
Oligodeoxynucleotide-containing phosphorothioate backbones have been used to regulate viral as well as cellular gene expression. The studies carried out in tissue culture have shown promising results on the use of oligonucleotide phosphorothioates as antiviral agents and, at present, study is underway to develop these oligonucleotide analogues as chemotherapeutic agents. To analyze and purify oligonucleotide analogues, high-performance liquid chromatography using weak anion exchange column has been described. The separation of oligonucleotide phosphorothioate is found to be length dependent.
Metelev
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Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
Agrawal
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D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification*; D013873:Thionucleotides / Q000302:isolation & purification*
D009838:Oligodeoxyribonucleotides; D013873:Thionucleotides