[The effect of the functional electrostimulation of rat fast and slow muscles on the structural state of actin in the thin filaments of a ghost muscle fiber].
The effect of electrostimulation of fast (EDL) and slow (SOL) rat muscles on the orientation and mobility of fluorescent probes rhodamine-phalloidine and 1.5-IAEDANS (N-iodoacetyl-N'-(5-sulpho-1-naphtyl)-ethylenediamine), located in various parts of actin molecule, has been studied by polarized microfluorimetry techniques. Muscles were stimulated at 20 Hz with the pulse width of 0.3 msec, some muscles were treated for 6 h during the first day, the other muscles for 6 h a day during the next 4 days before glycerinization. Then muscle fibres freed by the extraction of myosin, tropomyosin and troponin (ghost fibres) were used. It was shown that the binding of myosin subfragment 1 (S1) to actin induced the changes in polarized fluorescence of the fibres. The analysis of the obtained data showed that the formation of actomyosin complex in stimulated muscles resulted in increasing the angle between the thin filaments and the emission dipole of rhodamine-phalloidine, as well as in decreasing the mobility of this dye. In the experiments with the 1.5-IAEDANS label, the angle of the emission dipole decreased, while the label mobility increased. It was suggested that the orientation of domains in actomyosin complex changes following the electrostimulation to affect both the conformational state of F-actin in thin filaments of ghost fibres and actin-myosin interaction.
Kirillina
V P
VP
Borovikov
Iu S
IuS
Szczepanowska
J
J
Carraro
U
U
D008841:Actin Cytoskeleton / Q000502:physiology / Q000648:ultrastructure*; D000199:Actins / Q000502:physiology / Q000648:ultrastructure*; D000818:Animals; D003592:Cytophotometry; D004558:Electric Stimulation; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D009132:Muscles / Q000502:physiology*; D051381:Rats
D000199:Actins; D005456:Fluorescent Dyes
Vliianie funktsional'noĭ élektrostimuliatsii bystrykh i medlennykh myshts krysy na strukturnoe sostoianie aktina v tonkikh nitiakh tenevogo myshechnogo volokna.