Expression of antigen reactive with a monoclonal antibody to HTLV-1 P19 in salivary glands in Sjögren's syndrome.
To examine the possible involvement of retroviruses in Sjögren's syndrome (SS), labial salivary gland sections from 99 individuals were probed with three MoAbs to core (gag) proteins of human T cell leukaemia virus-1 (HTLV-1) and two MoAbs to HIV-1. Sections from 31% of 39 patients with primary SS (pSS) contained an epithelial cytoplasmic protein reactive with a MoAb (197) to the p19 group specific antigen (gag) of HTLV-1. The antigen was also detected in samples from 24% of 17 patients with rheumatoid arthritis (RA) and SS, 21% of 14 patients with sicca symptoms and 12.5% of 16 patients with other connective tissue diseases. It was not found in the salivary glands of 13 normal controls. A second MoAb to p19 gag, a MoAb to the p24 gag of HTLV-1 and MoAbs to HIV-1 p17 and p24 gags gave negative reactions. Serum antibodies to HTLV-1 were negative, confirming that the antigen was not part of HTLV-1. The antigen showed properties consistent with an endogenous retrovirus in that it was absent in healthy tissues or resting cells but inducible by stimulation with phytohaemagglutinin (PHA) or interferon-gamma (IFN-gamma). It appeared to be distinct from the endogenous retroviral sequence HRES-1. These data suggest the presence of an endogenous retrovirus in salivary gland epithelium which could contribute to the chronic inflammation of SS.
Shattles
W G
WG
Division of Clinical Immunology, Kennedy Institute of Rheumatology, London, UK.
Brookes
S M
SM
Venables
P J
PJ
Clark
D A
DA
Maini
R N
RN
D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000382:microbiology; D003240:Connective Tissue Diseases / Q000276:immunology; D003429:Cross Reactions; D004305:Dose-Response Relationship, Drug; D015683:Gene Products, gag / Q000096:biosynthesis*; D015488:HIV Antigens / Q000096:biosynthesis; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology; D010835:Phytohemagglutinins; D012191:Retroviridae Proteins / Q000096:biosynthesis; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis*; D012469:Salivary Glands / Q000276:immunology*; D012859:Sjogren's Syndrome / Q000276:immunology* / Q000382:microbiology*; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
C075728:197 antigen; D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D010835:Phytohemagglutinins; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1; C060955:p19 protein, Human T-lymphotropic virus 1; C060954:p24 protein, Human T-lymphotropic virus 1; D007371:Interferon-gamma