Isolation and characterization of alpha-glucosidase from Aspergillus niger.
alpha-Glucosidase is an enzyme widely used in biochemical analytical methods. Aspergillus niger was selected as a potential source for its production. Conditions for glucosidase production were optimized and the enzyme was isolated from the culture supernatant by dialysis and anion-exchange chromatography. The activity of the enzyme was determined by maltose hydrolysis to glucose, which was determined using a glucose-specific electrode or by high-performance liquid chromatography. The isolated enzyme was further characterized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, substrate specificity and fast protein liquid chromatography. The Michaelis constant, optimal temperature and stability of the enzyme preparation were determined.
Brízová
K
K
Department of Biochemistry, Institute of Chemical Technology, Prague, Czechoslovakia.
Králová
B
B
Demnerová
K
K
Vins
I
I
D000838:Anions; D001234:Aspergillus niger / Q000201:enzymology*; D002374:Catalase / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004566:Electrodes; D004800:Enzymes, Immobilized; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D013379:Substrate Specificity; D014462:Ultrafiltration; D000520:alpha-Glucosidases / Q000302:isolation & purification* / Q000378:metabolism
D000838:Anions; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D002374:Catalase; D000520:alpha-Glucosidases; D005947:Glucose