A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.
DNA repair was measured in an ionising radiation-sensitive mutant of a human bladder carcinoma cell line. No difference in the rate or extent of double-strand break rejoining was found using the techniques of neutral filter elution and pulsed-field gel electrophoresis. In contrast, significant differences in repair fidelity, measured by plasmid reconstitution, were found. The parent line had a repair fidelity of 84.7% compared with 58.9% for S40b (P = 0.0003). It is suggested that repair fidelity can be an important determinant of radiosensitivity in human tumour cells.
Powell
S N
SN
Radiotherapy Research Unit, Institute of Cancer Research, Sutton, Surrey, UK.
Whitaker
S J
SJ
Edwards
S M
SM
McMillan
T J
TJ
D002277:Carcinoma / Q000473:pathology / Q000503:physiopathology*; D004260:DNA Repair; D006801:Humans; D066298:In Vitro Techniques; D009154:Mutation; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D001749:Urinary Bladder Neoplasms / Q000473:pathology / Q000503:physiopathology*