Population cytogenetics of aphidicolin-induced fragile sites.
Chromosome fragile sites are inducible by aphidicolin in cultured human lymphocytes. To assess the frequency and distribution of these common fragile sites in the general population, a cytogenetic survey was performed on 126 subjects, 59 males and 67 females, whose age ranged from 1 day to 72 years. Common fragile sites, induced by aphidicolin, were widespread and showed a remarkably different sensitivity among individuals; age influenced the overall frequency of fragile sites. Moreover, both age and sex seemed to modulate the expression of specific fragile sites. In our population, the most common fragile sites were: 3p14, 16q23, Xp22, 6q26, 1p31, 4q31, 1p22, 7q22, 2q33, 3q27, 2q31, 7q32, 14q24, 10q22, 5q31, 2q37, 6p21.
Tedeschi
B
B
Department of Public Health and Cell Biology, II University of Rome, Tor Vergata, Italy.
Vernole
P
P
Sanna
M L
ML
Nicoletti
B
B
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D016590:Aphidicolin; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations / Q000235:genetics; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002877:Chromosomes, Human; D003582:Cytogenetics; D005260:Female; D005828:Genetics, Population; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012737:Sex Factors
D016590:Aphidicolin