Effects of reactive oxygen species on eicosanoid metabolism in human endothelial cells.
The influence of reactive oxygen species (H2O2 was used as model substance) on the formation and release of PGI2 and TXA2 by cultured human endothelial cells was analyzed. In the presence of H2O2 concentrations which did not induce a general cell damage (analyzed by estimation of the cellular concentration of energy rich phosphates and extent of lipid peroxidation), the formation of both eicosanoids exhibited a sigmoidal shape with respect to time. Increasing H2O2 concentration shortened the half time of PGI2 and TXA2 production. The maximum rates of PGI2 and TXA2 formation were separated by a delay of the TXA2 production. The ratio of PGI2 and TXA2 formation was 100 to 1 at the time of maximum PGI2 formation and 1-2 to 1 at the time of maximum TXA2 formation. This effect of reactive oxygen species could contribute to the reduction of the protective function of the endothelium in hemostasis and vascular tone. Using antioxidants, the modulating function of reactive oxygen species on the eicosanoid metabolism in endothelial cells was verified.
Schimke
I
I
Institute of Pathological and Clinical Biochemistry, Humboldt-University of Berlin, Germany.
Griesmacher
A
A
Weigel
G
G
Holzhutter
H G
HG
Muller
M M
MM
D000255:Adenosine Triphosphate / Q000378:metabolism; D002452:Cell Count; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D010725:Phosphocreatine / Q000378:metabolism; D013928:Thromboxane A2 / Q000096:biosynthesis*; D014471:Umbilical Veins
D010725:Phosphocreatine; D013928:Thromboxane A2; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide; D011464:Epoprostenol