A rapid method for cosmid cloning.
We present a method for genomic library construction using cosmid vectors. With a combination of backfilling with Klenow enzyme and a cosmid vector with two cos sites, a DNA bank in excess of 500,000 clones can be made from 10 micrograms of genomic DNA. The method is more rapid than conventional protocols because size fractionation of target DNA is not necessary. A further advantage is that libraries can be made from relatively small amounts of genomic DNA.
Loftus
M G
MG
Department of Biological Sciences, University of California, Santa Barbara 93106.
Foster
L M
LM
Ross
I K
IK
D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids; D004247:DNA / Q000235:genetics; D004256:DNA Polymerase I; D004271:DNA, Fungal / Q000235:genetics; D015698:Genomic Library
D004271:DNA, Fungal; D004247:DNA; D004256:DNA Polymerase I