Crystallization and preliminary X-ray diffraction analysis of staphylococcal enterotoxin type C.
The Type C staphylococcal enterotoxin produced by Staphylococcus aureus strain FRI-909 has been crystallized using a combination of two precipitants, ammonium sulfate and polyethylene glycol 400, with the addition of small amounts of detergent. Two related crystal forms have been obtained, one triclinic, and one tetragonal, both with one toxin molecule per asymmetric unit. These crystals are stable for at least 75 hr in the X-ray beam and diffract to at least 2.2 and 2.6 A, respectively. The triclinic crystals have unit cell parameters a = 38.5 A, b = 43.7 A, c = 36.9 A, and interaxial angles alpha = 99.9 degrees, beta = 95.8 degrees, and gamma = 98.5 degrees. The tetragonal crystals are of space group P4(1)22 with unit cell parameters a = 43.4 A and c = 278.0 A.
Bohach
G A
GA
Department of Bacteriology and Biochemistry, University of Idaho, Moscow 83843.
Chi
Y I
YI
Stauffacher
C V
CV
D003460:Crystallization; D004768:Enterotoxins / Q000737:chemistry*; D015394:Molecular Structure; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
D004768:Enterotoxins; C034959:enterotoxin C, staphylococcal