title: Detection of direct-acting mutagens in ambient air: a comparison of two highly sensitive mutagenicity assays.
abstract: Ambient air has been shown to contain numerous hazardous pollutants, many of which are known or suspected carcinogens and mutagens. Bioassays play a prominent role in the characterization of these genotoxic pollutants, and as new test methods are developed, it is incumbent upon researchers to evaluate assay performance and report relative merits. In this study, two Salmonella test methods (the spiral and preincubation assays) were assessed to determine their usefulness as screening methods for monitoring direct-acting mutagens in ambient air. The spiral assay automates the conventional plate-incorporation assay and has been shown to reduce the labor, materials, and sample mass required to perform mutagenicity testing. The preincubation assay has been shown to enhance test sensitivity for certain classes of compound, thereby reducing the amount of sample required for dose-response analysis. Both assays were used to test organic extracts of airborne particulate matter collected in Tokyo during the winters of 1988 and 1990. In addition to the conventional tester strains TA98 and TA100, two newly developed YG strains were evaluated. Strains YG1024 and YG1029-derived from TA98 and TA100, respectively-contain an acetyltransferase plasmid that confers upon the strains greater sensitivity towards nitroarenes. Results from this study indicated that both assays were able to detect direct-acting mutagens in the Tokyo air samples. The mutagenic activity associated with the samples was directly related to the particle mass present in a given volume of air. Mutagenic response was greater in the spiral assay relative to the preincubation assay, especially when YG tester strains were used. The YG strains were significantly more sensitive to mutation than the TA strains in both assays, which suggests that nitroaromatics are an important class of genotoxic contaminant present in Tokyo air.
authors.affiliation: U.S. Environmental Protection Agency, Health Effects Research Laboratory, Research Triangle Park, North Carolina 27711.
authors.forename: V S
authors.initials: VS
authors.lastname: Houk
authors.forename: S
authors.initials: S
authors.lastname: Goto
authors.forename: O
authors.initials: O
authors.lastname: Endo
authors.forename: L D
authors.initials: LD
authors.lastname: Claxton
authors.forename: J
authors.initials: J
authors.lastname: Lewtas
authors.forename: H
authors.initials: H
authors.lastname: Matsushita
mesh_terms: D000393:Air Pollutants / Q000032:analysis* / Q000633:toxicity; D007564:Japan; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000032:analysis*; D012486:Salmonella typhimurium / Q000187:drug effects; D012995:Solubility
chemical_list: D000393:Air Pollutants; D009153:Mutagens