Involvement of neuronal cell surface molecule B2 in the formation of retinal plexiform layers.
The B2 molecule is a 220 kd neuronal cell surface protein of Xenopus, recognized by monoclonal antibody B2 (MAb B2). Immunohistochemistry using MAb B2 revealed that the B2 molecule was expressed in both the inner and outer plexiform layers within the neural retina. During development of the neural retina, the B2 molecule first appeared at stages 35/36 in the newly formed plexiform layers. When embryonic eyes were cultured in the presence of anti-B2 antiserum (Fab fragments), the formation of the retinal plexiform layers was impeded. These data suggest that the cell surface molecule B2 plays a role in the development of retinal plexiform layers.
Ohta
K
K
Department of Molecular Life Science, School of Medicine, Kyushu University, Fukuoka, Japan.
Takagi
S
S
Asou
H
H
Fujisawa
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D002478:Cells, Cultured; D007140:Immunoglobulin Fab Fragments; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D009474:Neurons / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D009924:Organ Culture Techniques; D012160:Retina / Q000737:chemistry / Q000166:cytology / Q000196:embryology*; D014981:Xenopus
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D007140:Immunoglobulin Fab Fragments; D009419:Nerve Tissue Proteins; C091529:plexin