Spectral characteristics of cadmium-containing phytochelatin complexes isolated from Schizosaccharomyces pombe.
Phytochelatins, heavy-metal-containing peptides with structures (gamma EC)nG, where n = 2-8, have been isolated from higher plants and the fission yeast Schizosaccharomyces pombe. The present work describes the isolation and characterization of several naturally occurring mixed complexes of these peptides from S. pombe exposed to 1 mM CdCl2. A lower-molecular-mass fraction from Sephadex G-50 chromatography yielded three distinct species on further fractionation. HPLC chromatography revealed the presence of peptides with n = 1-4 in varying amounts in these three complexes, referred to as complexes I, II and III. Stoichiometries are proposed for these complexes, based on [Cd], [SH], [S2-] and the amino acid content. Ultraviolet absorption and magnetic circular dichroism spectra of complexes II and III are similar, whereas the CD spectra of these two complexes are strikingly different. Compared to both complexes II and III, the CD bands of complex I are relatively weak. Ultraviolet absorption, CD and magnetic circular dichroism spectra provide a basis for the discussion of structural differences in these complexes.
Plocke
D J
DJ
Department of Biology, Boston College, Massachusetts, Chesnut Hill 02167.
Kägi
J H
JH
D002104:Cadmium / Q000032:analysis*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D005978:Glutathione; D008667:Metalloproteins / Q000737:chemistry* / Q000302:isolation & purification; D054811:Phytochelatins; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D012568:Schizosaccharomyces / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D008667:Metalloproteins; D010940:Plant Proteins; D002104:Cadmium; D054811:Phytochelatins; D005978:Glutathione