Measurement of histamine in rat bronchoalveolar lavage fluid by high-performance cation-exchange chromatography coupled with post-column derivatization to o-phthaldialdehyde.
To better define involvement of mast cell derived mediators in the pulmonary response to fibrogenic dusts, a rapid and accurate method was required to analyze samples of bronchoalveolar lavage fluid for histamine. Samples of rat lung lavage were analyzed for histamine via high-performance cation-exchange chromatography coupled with post-column derivatization with o-phthaldialdehyde. The fluorescent derivative could be detected to ca. 1 ng/ml of lavage. Recoveries averaged 94.2% with an average relative standard deviation of +/- 5.3%. There were no correlations between amount or fibrogenicity of inhaled dust and subsequent release of histamine into lavage fluid.
Neubecker
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Miami Valley Laboratories, Procter and Gamble Company, Cincinnati, OH 45239-8707.
Miller
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Asquith
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Driscoll
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D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006632:Histamine / Q000378:metabolism*; D051381:Rats; D015203:Reproducibility of Results; D009764:o-Phthalaldehyde / Q000737:chemistry
D009764:o-Phthalaldehyde; D006632:Histamine