Endothelial EA.hy 926 cells can be loaded with cationized LDL, but not with acetylated LDL.
EA.hy 926 cells, a human endothelial cell line, show characteristics of differentiated endothelial cells. Endothelial cells normally express membrane scavenger receptors. Therefore modified LDL, eg., acetylated LDL, can be taken up, causing accumulation of mass amounts of cholesterol. We have shown that EA.hy 926 cells cannot be loaded with cholesterol using acetylated LDL, but can be efficiently enriched with cholesterol by incubation with cationized LDL. The loaded cells may serve as models for studies on reverse cholesterol transport.
Kilsdonk
E P
EP
Department of Biochemistry, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, Netherlands.
Dorsman
A N
AN
van Tol
A
A
D002460:Cell Line; D002784:Cholesterol / Q000032:analysis; D004727:Endothelium / Q000378:metabolism*; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D002784:Cholesterol