Enhancement of 1,3-propanediol production by cofermentation in Escherichia coli expressing Klebsiella pneumoniae dha regulon genes.
1,3-Propanediol (1,3-PD) is an intermediate in chemical and polymer synthesis. We have previously expressed the genes of a biochemical pathway responsible for 1,3-PD production, the dha regulon of Klebsiella pneumoniae, in Escherichia coli. An analysis of the maximum theoretical yield of 1,3-PD from glycerol indicates that the yield can be improved by the cofermentation of sugars, provided that kinetic constraints are overcome. The yield of 1,3-PD from glycerol was improved from 0.46 mol/mol with glycerol alone to 0.63 mol/mol with glucose cofermentation and 0.55 mol/mol with xylose cofermentation. The engineered E. coli also provides a model system for the study of metabolic pathway engineering.
Tong
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Department of Chemical Engineering, University of Wisconsin, Madison 53706-1691.
Cameron
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D000085:Acetates / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000235:genetics; D005561:Formates / Q000378:metabolism; D005818:Genetic Engineering; D005990:Glycerol / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000235:genetics*; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D011409:Propylene Glycols / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
D000085:Acetates; D005561:Formates; D006601:Hexoses; D007773:Lactates; D011409:Propylene Glycols; D013386:Succinates; C030544:formic acid; D019344:Lactic Acid; D000431:Ethanol; C041787:1,3-propanediol; D019802:Succinic Acid; D005990:Glycerol