title: Effect of different fractions of seminal plasma on the fertilizing ability of fowl spermatozoa stored in vitro.
abstract: This paper describes the effects of whole seminal plasma and of dialysed seminal plasma on the fertilizing ability of fowl spermatozoa stored for 24 h at 4 degrees C. The fertilizing ability of fowl semen diluted 1:1 with Beltsville Poultry Semen Extender and stored for 24 h at 4 degrees C was enhanced after replacement of the homologous seminal plasma by the diluent (89 versus 77% fertilization rate). Better results were obtained with seminal plasma dialysed against water before sperm storage to discard the less than 1 kDa or the less than 50 kDa fractions. It was concluded that low molecular weight seminal plasma fractions could damage the fertilizing ability of spermatozoa during storage at 4 degrees C, whereas high molecular weight fractions appeared to enhance fertilizing ability.
authors.affiliation: Station de Recherches Avicoles, INRA, Nouzilly, France.
authors.forename: E
authors.initials: E
authors.lastname: Blesbois
authors.forename: M
authors.initials: M
authors.lastname: de Reviers
mesh_terms: D000818:Animals; D002645:Chickens; D015925:Cryopreservation / Q000379:methods*; D005298:Fertility / Q000502:physiology*; D008297:Male; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa