Cyclic nucleotides alter intracellular free Mg2+ in renal epithelial cells.
Intracellular Mg2+ plays an important role in cell physiology. Studies were performed on MDCK cells and primary cortical thick ascending limb (CTAL) cells to determine hormonal influences on intracellular Mg2+ control. Free Mg2+ ([Mg2+]i) was measured by fluorescence with mag-fura-2. Addition of 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcG-MP, 10(-4) M) to subconfluent MDCK cells resulted in rapid increases in [Mg2+]i from basal levels of 552 +/- 6 microM to peak concentrations of 682 +/- 5 microM, whereas 8-BrcAMP (10(-4) M) led to significant decreases in [Mg2+]i from 538 +/- 5 to 362 +/- 17 microM. These effects of cyclic nucleotides were dose dependent with half-maximal concentrations (EC50) of approximately 10(-5) M for both increments in [Mg2+]i with cGMP and decrements in [Mg2+]i with cAMP. Atrial natriuretic peptide (ANP) and cGMP increased Mg2+ in porcine primary CTAL cells from 525 +/- 12 to 592 +/- 18 microM and from 538 +/- 8 to 609 +/- 18 microM, respectively. The increment in [Mg2+]i with ANP was dose responsive with EC50 values of approximately 10(-11) M suggesting that these effects may be of physiological importance. Parathyroid hormone and calcitonin and their second messenger, cAMP, diminished Mg2+ by approximately 80 microM. The EC50 value for calcitonin was in the order of 10(-9) M. The changes in [Mg2+]i, whether increases with ANP or cGMP and decreases with PTH, calcitonin, or cAMP, were rapid in nature and independent of changes in intracellular free Ca2+ concentration.(ABSTRACT TRUNCATED AT 250 WORDS)
Dai
L J
LJ
Department of Medicine, University of British Columbia, University Hospital, Vancouver, Canada.
Quamme
G A
GA
D000818:Animals; D002116:Calcitonin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism*; D009712:Nucleotides, Cyclic / Q000494:pharmacology*; D009994:Osmolar Concentration; D015290:Second Messenger Systems
D009712:Nucleotides, Cyclic; D002116:Calcitonin; D008274:Magnesium; D002118:Calcium