title: Crystallization and preliminary X-ray study of minor glucoamylase from Aspergillus awamori variant X-100/D27.
abstract: Crystals of the reduced form of glucoamylase were obtained from polyethylene glycol 6000 solution by the hanging-drop method. The protein was treated with alpha-mannosidase to partly remove the sugar component. The crystals belong to the space group P2(1)2(1)2(1) with cell dimensions a = 116.7 A, b = 104.3 A, c = 48.5 A and diffract beyond 2.5 A resolution.
authors.affiliation: Leningrad Nuclear Physics Institute, U.S.S.R. Academy of Sciences, Gatchina.
authors.forename: A M
authors.initials: AM
authors.lastname: Golubev
authors.forename: K N
authors.initials: KN
authors.lastname: Neustroev
authors.forename: A E
authors.initials: AE
authors.lastname: Aleshin
authors.forename: L M
authors.initials: LM
authors.lastname: Firsov
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D003460:Crystallization; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry* / Q000378:metabolism; D008361:Mannosidases / Q000378:metabolism; D011092:Polyethylene Glycols; D014961:X-Ray Diffraction; D043323:alpha-Mannosidase
chemical_list: D011092:Polyethylene Glycols; D008361:Mannosidases; D043323:alpha-Mannosidase; D005087:Glucan 1,4-alpha-Glucosidase