title: High resolution of multiple forms of red blood cell enzymes using a Toyopearl DEAE 650 S.
abstract: We have investigated a new anion exchange chromatographic support (Toyopearl DEAE 650 S) which simultaneously allows easily to remove hemoglobin from hemolysates and to obtain a very high resolution of enzymes present in multiple forms. The results obtained are better than those obtainable using an anion-exchange HPLC column. The data obtained at analytical level suggest a wider use of this new matrix also for preparative purposes without significant changes in the resolution.
authors.affiliation: Istituto di Chimica Biologica, Giorgio Fornaini, Università degli Studi, Urbino, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Stocchi
authors.forename: L
authors.initials: L
authors.lastname: Masat
authors.forename: B
authors.initials: B
authors.lastname: Biagiarelli
authors.forename: A
authors.initials: A
authors.lastname: Accorsi
authors.forename: G
authors.initials: G
authors.lastname: Piccoli
authors.forename: F
authors.initials: F
authors.lastname: Palma
authors.forename: L
authors.initials: L
authors.lastname: Cucchiarini
authors.forename: M
authors.initials: M
authors.lastname: Dachà
mesh_terms: D000818:Animals; D000837:Anion Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology*; D004983:Ethanolamines; D006593:Hexokinase / Q000302:isolation & purification; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification*; D011108:Polymers; D011683:Purine-Nucleoside Phosphorylase / Q000302:isolation & purification; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology; D013552:Swine
chemical_list: D000837:Anion Exchange Resins; C064803:DEAE-Toyopearl 650S; D004983:Ethanolamines; D007527:Isoenzymes; D011108:Polymers; D011683:Purine-Nucleoside Phosphorylase; D006593:Hexokinase