Genetic and morphological characterization of an Escherichia coli chromosome segregation mutant.
The temperature-sensitive nucleoid segregation mutant of Escherichia coli, PAT32, formerly described as a parA mutant, has been shown to carry a mutation near 66 min on the genetic map. Fine mapping with phages from the collection of Kohara et al. is consistent with its being a parC allele. Observation by fluorescence microscopy revealed the formation, at a nonpermissive temperature, of filaments containing one or two large nucleoids and of normal-size anucleate cells. There was also a significant loss of viability.
Stewart
P S
PS
Institut Jacques Monod, Centre National de la Recherche Scientifique, Université Paris 7, France.
D'Ari
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D002455:Cell Division; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D005819:Genetic Markers; D009154:Mutation; D013696:Temperature
D005819:Genetic Markers