Functional mapping of the rev-responsive element of human immunodeficiency virus type 2 (HIV-2): influence of HIV-2 envelope-encoding sequences on HIV-1 gp120 expression in the presence or absence of Rev.
The human immunodeficiency virus type 1 (HIV-1) regulatory protein Rev stimulates expression of structural viral proteins via a target response element (RRE) located within gag-pol and env mRNAs. To analyse the HIV-2 Rev trans-activation effect on the expression of the envelope protein, we cloned a functionally active HIV-2 rev cDNA and showed that it contained four exons. Using transient expression assays, we mapped a 353 bp RRE fragment within the env gene of HIV-2 on which both HIV-1 and HIV-2 Rev could act. Interestingly, smaller fragments suppressed the use of additional splice sites within the env gene and caused envelope protein expression independent of Rev.
Paulous
S
S
Unité d'Oncologie Virale (CNRS UA1157), Institut Pasteur, Paris, France.
Emerman
M
M
Keller
R
R
Montagnier
L
L
Cordonnier
A
A
D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015694:Gene Products, rev / Q000096:biosynthesis; D005814:Genes, Viral; D015757:Genes, rev; D015699:HIV Envelope Protein gp120 / Q000096:biosynthesis*; D015497:HIV-1 / Q000235:genetics; D015498:HIV-2 / Q000235:genetics*; D008969:Molecular Sequence Data; D014775:Virus Activation; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D015699:HIV Envelope Protein gp120; D054321:rev Gene Products, Human Immunodeficiency Virus