title: A reference preparation of human prostatic acid phosphatase: purification, characterization and field trials.
abstract: Acid phosphatase has been prepared in an apparently pure state by affinity chromatography from human prostatic tissue. When dissolved in an acidic albumin solution, lyophilized and stored at -20 degrees C for up to 2 years, no time-dependent loss of catalytic activity was detectable in the reconstituted material. Accelerated degradation tests also predicted complete stability. A preliminary distribution of the lyophilized preparation to 143 laboratories confirmed its robustness and demonstrated its potential usefulness as a calibrant to unify the results of different methods of measuring acid phosphatase activity.
authors.affiliation: Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Francis
authors.forename: D W
authors.initials: DW
authors.lastname: Moss
authors.forename: E
authors.initials: E
authors.lastname: Colinet
authors.forename: D H
authors.initials: DH
authors.lastname: Calam
authors.forename: D G
authors.initials: DG
authors.lastname: Bullock
mesh_terms: D000135:Acid Phosphatase / Q000302:isolation & purification* / Q000592:standards; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D008297:Male; D011467:Prostate / Q000201:enzymology*; D011506:Proteins / Q000032:analysis; D012016:Reference Values
chemical_list: D011506:Proteins; D000135:Acid Phosphatase