Evaluation of reagent strips for the rapid diagnosis of nitrite poisoning.
Two commercial urine test strips based on the Griess nitrite-specific diazonium salt reaction, having sulfanilamide and para-arsanilic acid as substrates, respectively, were studied as qualitative tests in the rapid diagnosis of nitrite/nitrate poisoning. Their usefulness was compared to other rapid tests, such as the sulfanilic acid-1 naphthylamin and diphenylamine blue tests. The practical sensitivity limit to nitrites in plasma and in water of both the reagent strips and SA-1NA test was 0.50 micrograms NO2/mL, while the diphenylamine test, which is not nitrite specific, showed a positive reaction to nitrites in plasma and in water above 50 micrograms NO2/mL and 5 micrograms NO2/mL, respectively. The in vitro assays were evaluated in vivo by the sublethal intoxication of a sheep with nitrite, demonstrating that commercial urine test strips may be useful for the rapid diagnosis of nitrite/nitrate poisoning.
Soler Rodríguez
F
F
Departamento de Medicina y Sanidad Animal, Facultad de Veterinaria, Cáceres, Spain.
Míguez Santiyán
M P
MP
Pedrera Zamorano
J D
JD
D005069:Evaluation Studies as Topic; D009573:Nitrites / Q000097:blood / Q000506:poisoning* / Q000652:urine; D011041:Poisoning / Q000097:blood / Q000175:diagnosis / Q000652:urine; D011934:Reagent Strips / Q000592:standards
D009573:Nitrites; D011934:Reagent Strips