title: Development of a high performance zinc-62/copper-62 radionuclide generator for positron emission tomography.
abstract: Clinical utilisation of positron emission tomography could be enhanced by the availability of short-lived radionuclides derived from generator systems. The zinc-62/copper-62 combination is one such system which could be used as a source for a number of copper-62 radiopharmaceuticals. We have developed and optimised a high activity (5.6 GBq, 150 mCi) zinc-62/copper-62 generator to provide 62Cu in a form that is suitable for direct labelling of pyruvaldehyde-bis-(N4-methylthiosemicarbazone)-copper(II), Cu(PTSM). The distribution coefficients of Zn(II) and Cu(II) between anion-exchange resin and various hydrochloric acid/organic solvent mixtures were measured. Based on these measurements a generator eluent of 0.3 M HCl/40% ethanol provided 62Cu in greater than 90% yield in a 3-ml volume. A very low 62Zn breakthrough of less than 3 x 10(-7)% was achieved. Copper-PTSM was successfully labelled with the no-carrier-added 62Cu eluent directly from the generator with 94% radiochemical yield.
authors.affiliation: Joint Department of Physics, Royal Marsden Hospital, Sutton, Surrey, UK.
authors.forename: J
authors.initials: J
authors.lastname: Zweit
authors.forename: R
authors.initials: R
authors.lastname: Goodall
authors.forename: M
authors.initials: M
authors.lastname: Cox
authors.forename: J W
authors.initials: JW
authors.lastname: Babich
authors.forename: G A
authors.initials: GA
authors.lastname: Potter
authors.forename: H L
authors.initials: HL
authors.lastname: Sharma
authors.forename: R J
authors.initials: RJ
authors.lastname: Ott
mesh_terms: D003300:Copper; D003301:Copper Radioisotopes; D006801:Humans; D007553:Isotope Labeling; D009942:Organometallic Compounds; D011876:Radionuclide Generators; D013882:Thiosemicarbazones; D014055:Tomography, Emission-Computed; D015037:Zinc Radioisotopes
chemical_list: D003301:Copper Radioisotopes; D009942:Organometallic Compounds; D013882:Thiosemicarbazones; D015037:Zinc Radioisotopes; C052629:copper pyruvaldehyde bis(N(4)-methylthiosemicarbazone) complex; D003300:Copper