title: mmCGM1a: a mouse carcinoembryonic antigen gene family member, generated by alternative splicing, functions as an adhesion molecule.
abstract: Carcinoembryonic antigen is a human tumor marker and the prototype of a large family of immunoglobulin-like proteins. We have been developing a mouse model for this large protein family and have cloned a complementary DNA (cDNA) for a mouse carcinoembryonic antigen gene family member (mmCGM1a). Two transcripts expressed in several different adult mouse tissues hybridize to this cDNA, a 1.8-kilobase and a 4.6-kilobase mRNA. Sequences of many related cDNA clones indicate that they are most likely encoded by a single gene which undergoes alternative splicing. The protein encoded by the mmCGM1a cDNA shares 69% of the amino acid residues in the NH2-terminal domain with a rat liver ecto-ATPase and with the human biliary glycoprotein. Mouse fibroblast transfectant cells expressing the mmCGM1a protein on their cell surface exhibit calcium- and temperature-independent adhesion in vitro which can be specifically inhibited by an antibody raised against a carcinoembryonic antigen-related 120 kilodalton protein.
authors.affiliation: McGill Cancer Centre, McGill University, Montreal, Canada.
authors.forename: K
authors.initials: K
authors.lastname: McCuaig
authors.forename: C
authors.initials: C
authors.lastname: Turbide
authors.forename: N
authors.initials: N
authors.lastname: Beauchemin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000235:genetics* / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D016373:DNA, Antisense / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D012326:RNA Splicing / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic / Q000235:genetics; D014162:Transfection / Q000235:genetics
chemical_list: C075729:CEACAM3 protein, human; D002272:Carcinoembryonic Antigen; C486183:Ceacam1 protein, mouse; D015815:Cell Adhesion Molecules; D016373:DNA, Antisense; D004247:DNA