Direct microtiter plate sequencing of PCR-amplified M13 clones from plaques using dried reagents.
A method is described using dried reagents in microtiter plates for direct PCR and sequencing of M13 plaques. The method has advantages of reliability and high throughput that make it suitable for medium to large-scale sequencing projects.
Dale
S J
SJ
Amersham International plc, Little Chalfont, Bucks, UK.
D001483:Base Sequence; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D003890:Desiccation; D008832:Microchemistry / Q000295:instrumentation; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping / Q000379:methods*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral