title: Toxic effects of solstitialin A 13-acetate and cynaropicrin from Centaurea solstitialis L. (Asteraceae) in cell cultures of foetal rat brain.
abstract: Extracts of the aerial parts of the yellow star thistle (Centaurea solstitialis L.) were prepared using petroleum ether, chloroform and methanol and toxicity assessed in primary cell cultures of striatum, frontal cortex and mesencephalon, containing the substantia nigra or raphe nucleus from the brain of the foetal rat. Chloroform extracts significantly reduced the percentage of live cells whereas the petroleum ether extract was inactive. The ability of the methanol extract to reduce the percentage of live cells could not be distinguished from the effects of the vehicle controls for methanol. Subsequently, the plant material was extracted with dichloromethane and 10 fractions were obtained by column chromatography. Fractions 5, 6, 7 and 8 caused concentration-dependent cell death in the culture of substantia nigra, the other fractions were not toxic at the concentrations used. In further studies, solstitialin A was isolated from fraction 9, solstitialin A 13-acetate from fraction 6 and solstitialin A-3 acetate and cynaropicrin from fraction 7. Solstitialin A 13-acetate and cynaropicrin were toxic to cultures of substantia nigra cells. It is concluded that, of the compounds identified, solstitialin A 13-acetate and cynaropicrin have toxic potential in cell cultures, containing cells from the substantia nigra of the rat, the specificity of action to cells of the substantia nigra remains to be shown, and that a toxic action in the midbrain may contribute to the nigro-pallidal encephalomalacia, caused by the ingestion of the yellow star thistle by horses.
authors.affiliation: Postgraduate Studies in Pharmacology, School of Pharmacy, University of Bradford, West Yorkshire, U.K.
authors.forename: C H
authors.initials: CH
authors.lastname: Cheng
authors.forename: B
authors.initials: B
authors.lastname: Costall
authors.forename: M
authors.initials: M
authors.lastname: Hamburger
authors.forename: K
authors.initials: K
authors.lastname: Hostettmann
authors.forename: R J
authors.initials: RJ
authors.lastname: Naylor
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Jenner
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D007783:Lactones / Q000008:administration & dosage / Q000494:pharmacology*; D010704:Phorbols; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D010944:Plants / Q000737:chemistry*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D012717:Sesquiterpenes / Q000494:pharmacology* / Q000633:toxicity*
chemical_list: D007783:Lactones; D010704:Phorbols; D010936:Plant Extracts; D012717:Sesquiterpenes; C003325:solstitialin; C075686:solstitialin A 13-acetate; C075687:cynaropicrin