title: The combination of normal phase with reversed phase high performance liquid chromatography for the analysis of asparagine-linked neutral oligosaccharides labelled with p-aminobenzoic ethyl ester.
abstract: We have developed a novel approach for the analysis of asparagine-linked neutral oligosaccharides derived from glycoproteins. The oligosaccharides are labelled with p-aminobenzoic ethyl ester and the derivatives are separated on two high performance liquid chromatographic columns, one containing amide-silica and the other containing octadecyl-silica. The elution positions of 39 different ABEE-oligosaccharides on the two columns were plotted on a two-dimensional map. Unique non-overlapping positions of these oligosaccharides demonstrate that this technology would be useful for the identification of Asn-linked oligosaccharides at high sensitivity.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Fukuyama University, Hiroshima, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Matsuura
authors.forename: M
authors.initials: M
authors.lastname: Ohta
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Hirano
authors.forename: C C
authors.initials: CC
authors.lastname: Sweeley
mesh_terms: D001216:Asparagine / Q000032:analysis*; D001566:Benzocaine / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D009844:Oligosaccharides / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D009844:Oligosaccharides; D001216:Asparagine; D001566:Benzocaine