In vivo function of regulatory DNA sequence elements of a major histocompatibility complex class I gene.
Major histocompatibility complex class I genes are expressed in nearly all somatic tissues, although their level of expression varies. By analysis of a set of promoter deletion mutants introduced into transgenic mice, a complex regulatory element, consisting of overlapping enhancer and silencer activities, is demonstrated to function as a tissue-specific regulator of class I expression. The enhancer activity predominates in lymphoid tissues but not in nonlymphoid tissues. In contrast to the tissue-specific functions of the complex regulatory element, a second novel silencer element is shown to function in both lymphoid and nonlymphoid tissues. The complement of DNA-binding factors in different cell lines is shown to correlate with the levels of class I expression.
Maguire
J E
JE
Experimental Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.
Frels
W I
WI
Richardson
J C
JC
Weissman
J D
JD
Singer
D S
DS
D000818:Animals; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D005805:Genes, MHC Class I / Q000235:genetics*; D008221:Lymphoid Tissue / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*
D004268:DNA-Binding Proteins; C075396:PD1 protein, pig; D011994:Recombinant Proteins