Expression of glycoconjugates in the mouse inner ear after prenatal irradiation.
Irradiation of the murine fetal inner ear is known to produce damage both to the vestibular and cochlear parts in the adult mouse. Fluorescein-labelled lectins were used to reveal possible differences in the glycoconjugate content between normal and irradiated inner ears. In the vestibular part, the otoconia showed the highest uptake of labelled sugars. This uptake was weaker after irradiation when compared to non-irradiated specimens. The type I hair cells in the ampulla and in the utricle showed a weaker uptake, but no labelling was demonstrated in the type II hair cells compared to the non-irradiated controls. In the cochlear part of the inner ear almost no uptake of fluorescent-binding lectins could be demonstrated in the irradiated groups except for in the tectorial membrane. In the endolymphatic sac no uptake was shown after prenatal irradiation. These findings are discussed and correlated to the already known damage of the inner ear following prenatal irradiation.
Hultcrantz
M
M
Department of Otorhinolaryngology, Karolinska Hospital, Stockholm, Sweden.
D000818:Animals; D050260:Carbohydrate Metabolism; D003051:Cochlea / Q000378:metabolism / Q000528:radiation effects; D007758:Ear, Inner / Q000378:metabolism* / Q000528:radiation effects*; D005260:Female; D005333:Fetus / Q000528:radiation effects; D006001:Glycoconjugates / Q000378:metabolism* / Q000528:radiation effects*; D006198:Hair Cells, Auditory / Q000528:radiation effects; D037102:Lectins / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D011247:Pregnancy; D014722:Vestibule, Labyrinth / Q000378:metabolism / Q000528:radiation effects
D006001:Glycoconjugates; D037102:Lectins