High-pressure, high-temperature bioreactor for comparing effects of hyperbaric and hydrostatic pressure on bacterial growth.
We describe a high-pressure reactor system suitable for simultaneous hyperbaric and hydrostatic pressurization of bacterial cultures at elevated temperatures. For the deep-sea thermophile ES4, the growth rate at 500 atm (1 atm = 101.29 kPa) and 95 degrees C under hydrostatic pressure was ca. three times the growth rate under hyperbaric pressure and ca. 40% higher than the growth rate at 35 atm.
Nelson
C M
CM
Department of Chemical Engineering, University of California, Berkeley 94720-9989.
Schuppenhauer
M R
MR
Clark
D S
DS
D001419:Bacteria / Q000254:growth & development*; D001709:Biotechnology / Q000295:instrumentation*; D006371:Helium; D006874:Hydrostatic Pressure; D013696:Temperature
D006371:Helium