Determination of 8-methoxypsoralen in plasma by gas chromatography-mass spectrometry using selected-ion monitoring.
A sensitive and accurate assay was developed for the measurement of 8-methoxypsoralen in plasma using electron-impact positive-ion mass fragmentography. 4,5,8-Trimethylpsoralen was used as an internal standard. Sample preparation consisted of a two-step liquid phase extraction using acetonitrile and methylene chloride. The calibration curve showed a linear relationship between the peak areas of 8-methoxypsoralen and 4,5,8-trimethylpsoralen over a wide range of 8-methoxypsoralen concentrations (1-500 ng/ml). With-in- and between-run precisions, measured at five different drug concentrations, varied from 0.82 to 1.41% and from 0.82 to 1.86%, respectively.
Cracco
A T
AT
Department of Pediatrics, University of Padua, Italy.
Dall'Amico
R
R
Ferrari
V
V
Bottaro
S
S
Zacchello
G
G
Chiandetti
L
L
Zacchello
F
F
D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007477:Ions; D008730:Methoxsalen / Q000378:metabolism* / Q000627:therapeutic use; D010778:Photochemotherapy
D007477:Ions; D008730:Methoxsalen