Activation and phenotyping of LAK generated by exposure to product of cells transformed by interleukin 2 cDNA.
It has been previously found that local administration of X63-m-IL-2 cells transformed by interleukin 2 (IL-2) cDNA and constitutively producing large quantities of IL-2 mediated regressions of murine plasmacytomas and 3-methyl-cholanthrene-induced sarcomas transplanted in syngeneic mice. Here we show that killer cells generated by cultivation of spleen cells in supernatants from X63-m-IL-2 cultures (LAK) or by co-cultivation of murine splenocytes with X63-m-IL-2 cells were cytolytic for natural killer (NK)-sensitive as well as NK-resistant target cells, including the IL-2-producing X63-m-IL-2 cells. Spleen cells cultured in X63-m-IL-2 supernatants or co-cultivated with X63-m-IL-2 cells yielded predominantly Thy 1.2+, CD3+, LFA-1+ lymphocytes. The in vitro results suggest that the LAK cells generated due to the IL-2 production by genetically engineered cells probably help to terminate the treatment by killing the IL-2 producers.
Bubeník
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Zeuthen
J
J
Símová
J
J
Jandlová
T
T
Bubeníková
D
D
Radzikowski
C
C
D000818:Animals; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004247:DNA / Q000235:genetics*; D005434:Flow Cytometry; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D013154:Spleen / Q000276:immunology; D014162:Transfection
D007376:Interleukin-2; D004247:DNA