Simultaneous determination of hydrogen, methane and carbon dioxide of breath using gas-solid chromatography.
The analysis of respiratory hydrogen and methane was estimated as a useful index of intestinal fermentation of undigestible carbohydrate. A simultaneous and precious analysis of these gases as well as carbon dioxide was studied. A gas-impermeable multi-laminated film bag metalized by aluminum vapor was fitted to use as a storage bag; its impermeability was verified by measuring the residual rate of hydrogen after 3 months' storage. Hydrogen, methane and carbon dioxide of the breath gas even at 1 ppm could be determined simultaneously by using a gas-solid chromatography installed with a photoionization detector and active carbon column. To observe the genesis of hydrogen and methane after carbohydrate ingestion, pectin, a typical water-soluble dietary fiber, was fed to healthy volunteers. Increasing excretion of pulmonary hydrogen or methane showed the sign of intestinal fermentation as the results of carbohydrate malabsorption.
Tsuji
K
K
Department of Applied Food Research, National Institute of Health and Nutrition, Tokyo, Japan.
Shimizu
M
M
Nishimura
Y
Y
Nakagawa
Y
Y
Ichikawa
T
T
D000328:Adult; D001944:Breath Tests / Q000379:methods*; D002245:Carbon Dioxide / Q000032:analysis*; D002849:Chromatography, Gas; D006801:Humans; D006859:Hydrogen / Q000032:analysis*; D008297:Male; D008697:Methane / Q000032:analysis*; D008875:Middle Aged
D002245:Carbon Dioxide; D006859:Hydrogen; D008697:Methane