title: Measurement of histamine in rat bronchoalveolar lavage fluid by high-performance cation-exchange chromatography coupled with post-column derivatization to o-phthaldialdehyde.
abstract: To better define involvement of mast cell derived mediators in the pulmonary response to fibrogenic dusts, a rapid and accurate method was required to analyze samples of bronchoalveolar lavage fluid for histamine. Samples of rat lung lavage were analyzed for histamine via high-performance cation-exchange chromatography coupled with post-column derivatization with o-phthaldialdehyde. The fluorescent derivative could be detected to ca. 1 ng/ml of lavage. Recoveries averaged 94.2% with an average relative standard deviation of +/- 5.3%. There were no correlations between amount or fibrogenicity of inhaled dust and subsequent release of histamine into lavage fluid.
authors.affiliation: Miami Valley Laboratories, Procter and Gamble Company, Cincinnati, OH 45239-8707.
authors.forename: T A
authors.initials: TA
authors.lastname: Neubecker
authors.forename: N S
authors.initials: NS
authors.lastname: Miller
authors.forename: T N
authors.initials: TN
authors.lastname: Asquith
authors.forename: K E
authors.initials: KE
authors.lastname: Driscoll
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006632:Histamine / Q000378:metabolism*; D051381:Rats; D015203:Reproducibility of Results; D009764:o-Phthalaldehyde / Q000737:chemistry
chemical_list: D009764:o-Phthalaldehyde; D006632:Histamine