Synthesis of a gene encoding bovine substance P precursors and its expression in Escherichia coli.
Using an efficient Escherichia coli expression system, we have been able to obtain the precursor of substance P, alpha-preprotachykinin (alpha PPT). The alpha PPT protein is produced in E. coli as a fusion to beta-galactosidase, and accumulates in the cytoplasm as insoluble inclusion bodies. We also produced protachykinin (alpha PT), i.e., alpha PPT without a signal peptide. Further purification and characterization of the alpha PPT and alpha PT polypeptides strongly suggest that fully purified products can be obtained using our procedures.
Hrabec
E
E
Laboratory of Genetic Engineering, Sieradz, Poland.
Hrabec
Z
Z
Płucienniczak
G
G
Skorupa
G
G
Szczepanek
A
A
Płucienniczak
A
A
Uznański
B
B
Wilk
A
A
Okruszek
A
A
Koziołkiewicz
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005813:Genes, Synthetic / Q000235:genetics; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015320:Tachykinins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
D011498:Protein Precursors; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D015320:Tachykinins; C044206:preprotachykinin; C054618:protachykinin