Liquid chromatography-mass spectrometry of hydroxy and non-hydroxy fatty acids as amide derivatives.
A useful method for analyzing fatty acids by liquid chromatography-mass spectrometry with an atmospheric-pressure chemical-ionization interface system has been developed. The sensitivity of six kinds of palmitamide derivatives monitored by a single ion of [M+H]+ was, in decreasing order: N-n-propylamide greater than anilide greater than N,N-diethylamide, amide greater than N,N-diphenylamide greater than N-1-naphthylamide. Individual fatty acids were identified from a mixture of amide derivatives of authentic fatty acids from C16:0 to C30:0 on a mass chromatogram. This method was used to detect both hydroxy and non-hydroxy fatty acids. Many kinds of fatty acid, including hydroxy fatty acids of the rat brain, were detected in a single run.
Ikeda
M
M
Department of Biochemistry, Kawasaki Medical School, Okayama, Japan.
Kusaka
T
T
D000818:Animals; D001923:Brain Chemistry; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006880:Hydroxy Acids / Q000378:metabolism; D008297:Male; D010169:Palmitic Acids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D005227:Fatty Acids; D006880:Hydroxy Acids; D010169:Palmitic Acids