title: Induction of the POU domain transcription factor Oct-2 during T-cell activation by cognate antigen.
abstract: Oct-2 is a transcription factor that binds specifically to octamer DNA motifs in the promoters of immunoglobulin and interleukin-2 genes. All tumor cell lines from the B-cell lineage and a few from the T-cell lineage express Oct-2. To address the role of Oct-2 in the T-cell lineage, we studied the expression of Oct-2 mRNA and protein in nontransformed human and mouse T cells. Oct-2 was found in CD4+ and CD8+ T cells prepared from human peripheral blood and in mouse lymph node T cells. In a T-cell clone specific for pigeon cytochrome c in the context of I-Ek, Oct-2 was induced by antigen stimulation, with the increase in Oct-2 protein seen first at 3 h after activation and continuing for at least 24 h. Oct-2 mRNA induction during antigen-driven T-cell activation was blocked by cyclosporin A, as well as by protein synthesis inhibitors. These results suggest that Oct-2 participates in transcriptional regulation during T-cell activation. The relatively delayed kinetics of Oct-2 induction suggests that Oct-2 mediates the changes in gene expression which occur many hours or days following antigen stimulation of T lymphocytes.
authors.affiliation: Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
authors.forename: S M
authors.initials: SM
authors.lastname: Kang
authors.forename: W
authors.initials: W
authors.lastname: Tsang
authors.forename: S
authors.initials: S
authors.lastname: Doll
authors.forename: P
authors.initials: P
authors.lastname: Scherle
authors.forename: H S
authors.initials: HS
authors.lastname: Ko
authors.forename: A C
authors.initials: AC
authors.lastname: Tran
authors.forename: M J
authors.initials: MJ
authors.lastname: Lenardo
authors.forename: L M
authors.initials: LM
authors.lastname: Staudt
mesh_terms: D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002999:Clone Cells; D016572:Cyclosporine / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D050813:Octamer Transcription Factor-2; D012333:RNA, Messenger / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D004268:DNA-Binding Proteins; D050813:Octamer Transcription Factor-2; C058435:POU2F2 protein, human; C489206:Pou2f2 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors; D016572:Cyclosporine