Semi-automated assay of erythrocyte Cu-Zn superoxide dismutase activity.
A semi-automated enzymatic colorimetric method for determining Cu-Zn superoxide dismutase (SOD) in erythrocytes using an FP9 parallel analyzer is described. The method is based on the reaction described by McCord and Fridovich (xanthine/xanthine oxidase, ferricytochrome C), which permits kinetic measurements to be determined colorimetrically for the assay of enzyme activity. The coefficients of variation for within- and between-run analyses were less than 6%. The limit of linearity is 3.5-times the mean value of the normal range while recovery of added SOD ranged from 91 to 120%. The method, which is both reliable and simple, allows rapid, simultaneous measurements of multiple samples.
Fulbert
J C
JC
Laboratoire de Biochimie, Hôpital Corentin Celton, Issy les Moulineaux, France.
Succari
M
M
Cals
M J
MJ
D000328:Adult; D001322:Autoanalysis / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results; D013482:Superoxide Dismutase / Q000032:analysis*
D013482:Superoxide Dismutase