Comparison of a novel thin-layer chromatographic-fluorescence detection method with a spectrofluorometric method for the determination of 7-hydroxycoumarin in human urine.
A novel method for the determination of 7-hydroxycoumarin in human urine which combines thin-layer chromatography (TLC) with fluorescence detection (FD) has been devised. The limit of detection (1 ng/ml) enables determination of 7-hydroxycoumarin after both administration of coumarin and environmental exposure to this fragrance material. When compared to a spectrofluorometric method of analysis, the TLC-FD method proved to be more selective for the analysis of 7-hydroxycoumarin in human urine.
Cholerton
S
S
Department of Pharmacological Sciences, University of Newcastle upon Tyne, UK.
Idle
M E
ME
Vas
A
A
Gonzalez
F J
FJ
Idle
J R
JR
D000284:Administration, Oral; D002855:Chromatography, Thin Layer / Q000379:methods*; D003374:Coumarins / Q000008:administration & dosage; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014468:Umbelliferones / Q000652:urine*
D003374:Coumarins; D014468:Umbelliferones; C030123:coumarin