A group additivity model for analyzing absorption spectra of organic compounds: applications to partial structural analysis and molecular weight determinations of polymers, nucleotides, and peptides.
A new method for the analysis of organic structural groups from absorption spectra is described. The method is based on the integrated intensity of absorption. It requires only micrograms of analyzed compound, which may be fully recovered after analysis from its solution. The method can be used for different kinds of structural and/or kinetic studies. For example, the reaction kinetics and tautomeric equilibria can be easily studied by this method. The method can also be used for the determination of molecular weight and quantitative composition of polymers, nucleotides, and/or peptides. Hydrolysis or derivatization of the studied compounds is not necessary. On the basis of this method, automatic molecular weight, nucleotide, and peptide analyzers can be constructed.
Chrastil
J
J
U.S. Department of Agriculture, Southern Regional Research Center, New Orleans, Louisiana 70179.
D006863:Hydrogen-Ion Concentration; D008433:Mathematics; D008970:Molecular Weight; D009711:Nucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013057:Spectrum Analysis
D009711:Nucleotides; D010455:Peptides; D011108:Polymers