Platelet hypoaggregability in rats exposed to SART stress (repeated cold stress).
A prolongation of bleeding time accompanied by thrombocytopenia and abnormalities in coagulation-fibrinolysis systems has been observed in laboratory animals exposed to a chronic form of environmental stress induced by severe fluctuations of air temperature, known as SART (specific alternation of rhythm in temperature) stress. In order to clarify the hemostatic profile under SART stress in more detail, the present study examined platelet aggregability in vitro as well as in vivo in stressed rats. During exposure to stress, thrombocytopenia developed from day 5, and remained up to at least day 14. In vitro aggregation of platelets stimulated by ADP or collagen was markedly decreased in stressed rats, compared with unstressed rats. Furthermore, stressed rats exhibited in vivo hypoaggregability of platelets, as estimated by the magnitude of the drop in circulating platelet counts following intravenous injection of ADP and collagen. Protein and cholesterol content in platelets remained constant after stress exposure. These results indicate that SART-stressed rats exhibit platelet dysfunctions in addition to thrombocytopenia. Considering the previous findings, the hemostatic system under SART stress appears to show a general tendency toward hemorrhage.
Hata
T
T
Department of Pharmacology, Faculty of Pharmacy, Kinki University, Osaka, Japan.
Kawabata
A
A
Itoh
E
E
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D003080:Cold Temperature; D003094:Collagen / Q000494:pharmacology; D006487:Hemostasis / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013312:Stress, Physiological / Q000097:blood*
D000244:Adenosine Diphosphate; D003094:Collagen