Extension of the DNA binding consensus of the chicken c-Myb and v-Myb proteins.
The chicken c-myb gene and the v-myb oncogene transduced by avian myeloblastosis virus (AMV) encode DNA binding transcription activators. The DNA binding domain of AMV v-Myb displays a number of amino acid changes relative to c-Myb; v-Myb proteins in which one or more of three crucial residues in the DNA binding domain are mutated to resemble the c-Myb sequence display altered transformation phenotypes. In order to establish whether the spectrum of DNA binding sites which AMV v-Myb can recognise is different from that seen by chicken c-Myb, a site selection protocol was used to derive consensus binding sequences for three variant Myb proteins made in vitro, and also using nuclear extract from the v-myb transformed cell line BM2. The results show that the original consensus binding site defined for v-Myb, YAA-CKG, can be extended to YAACKGHH, and that this new consensus holds for both v-Myb and chicken c-Myb.
Weston
K
K
Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
D000595:Amino Acid Sequence; D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002645:Chickens / Q000235:genetics; D016384:Consensus Sequence / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D020626:Oncogene Proteins v-myb; D009857:Oncogenes; D010957:Plasmids / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000235:genetics*; D020598:Proto-Oncogene Proteins c-myb; D015682:Retroviridae Proteins, Oncogenic / Q000737:chemistry / Q000235:genetics*
D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D020626:Oncogene Proteins v-myb; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D015682:Retroviridae Proteins, Oncogenic