title: Mechanisms of vagal nerve in gastric mucosal defense: unchanged gastric emptying and increased vascular permeability.
abstract: Gastric cytoprotection in response to different agents (prostaglandins, carotenoids, etc.) failed to occur after surgical vagotomy. Decreased gastric emptying and the increased vascular permeability were tested in ethanol-treated rats without and with bilateral surgical vagotomy. The experiments were carried out on Sprague-Dawley rats. The animals were fasted for 24 h before experiments. Bilateral surgical vagotomy or only laparatomy were carried out at 30 min before administration of ethanol (96%, 1 ml). The animals were killed at 0, 1, 5, 15, and 60 min after ethanol administration, when the number and severity of gastric mucosal lesions were noted. In another series of experiments, the animal received Evans blue (1 mg/100 g) i.v. 15 min before killing. The gastric contents were collected and the glandular mucosa was scraped. Evans blue was extracted in chloroform, and its concentration was spectrophotometrically measured. It has been found that (a) both number of lesions and severity of ethanol-induced gastric mucosal damage were larger at each time period in surgically vagotomized rats than in rats with intact vagal nerves; (b) the increased vascular permeability was significantly higher in gastric mucosa at an early period in surgically vagotomized rats compared to rats with intact vagal nerve; (c) the increased vascular events preceded the development of macroscopic appearance of gastric mucosa damage in both groups of animals; and (d) the time-related responses were the same in both groups of animals. It is concluded that increased vascular permeability, but not gastric emptying, probably has some role in the failure of the development of gastric cytoprotection in surgically vagotomized rats.
authors.affiliation: First Department of Medicine, Medical University of Pécs, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Mózsik
authors.forename: A
authors.initials: A
authors.lastname: Király
authors.forename: M
authors.initials: M
authors.lastname: Garamszegi
authors.forename: T
authors.initials: T
authors.lastname: Jávor
authors.forename: L
authors.initials: L
authors.lastname: Nagy
authors.forename: A
authors.initials: A
authors.lastname: Németh
authors.forename: G
authors.initials: G
authors.lastname: Sütö
authors.forename: A
authors.initials: A
authors.lastname: Vincze
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000502:physiology*; D000431:Ethanol / Q000009:adverse effects; D005746:Gastric Emptying / Q000502:physiology*; D005753:Gastric Mucosa / Q000294:innervation* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000139:chemically induced / Q000503:physiopathology*; D014628:Vagotomy; D014630:Vagus Nerve / Q000502:physiology*
chemical_list: D000431:Ethanol