title: The promoter element GTACAAG of the SGA and STA2 genes is a possible target site for repression by the STA10 gene product from Saccharomyces cerevisiae.
abstract: The SGA and STA2 genes that, respectively, encode the intra- and extracellular glucoamylases of Saccharomyces cerevisiae are coregulated negatively, at the level of transcription, by the STA10 gene. This finding was re-examined by determining the effects of STA10 on the expression of gene constructs containing different fragments from the SGA and STA2 promoter regions fused to the lacZ gene. Repression was observed only for promoter fragments carrying the sequence GTACAAG indicating that this element is responsible for the coregulation of SGA and STA2 by STA10.
authors.affiliation: Centro de Biología Molecular, Universidad Autónoma, Madrid, Spain.
authors.forename: M G
authors.initials: MG
authors.lastname: Claros
authors.forename: L
authors.initials: L
authors.lastname: del Pozo
authors.forename: D
authors.initials: D
authors.lastname: Abarca
authors.forename: A
authors.initials: A
authors.lastname: Jiménez
mesh_terms: D001483:Base Sequence; D004252:DNA Mutational Analysis; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D012097:Repressor Proteins; D001616:beta-Galactosidase; D005087:Glucan 1,4-alpha-Glucosidase