The effect of serum concentrations and sources on DNA synthesis and insulin secretion of cultured islets.
We investigated the effect of different sera, as newborn calf serum, fetal calf serum, rat serum and human serum at various concentrations on biofunctions of cultured pancreatic islets. Islets isolated from newborn LEW. 1 W rats were maintained at 37 degrees C in TCM 199 containing 10 mmol/l glucose and either 1%, 10% or 50% newborn calf serum (NCS), fetal calf serum (FCS), serum obtained from adult syngeneic rats (RS) or different batches of human serum (HS). While exposure of islets to increasing concentrations of NCS significantly inhibited the islet DNA synthesis as well as the glucose stimulated insulin secretion after 2, 4 and 8 days of culture, HS at different concentrations failed to inhibit both, the islet DNA synthesis and the secretory response to glucose at each time point investigated. The action of FCS, RS and 2 further batches of HS on islet DNA synthesis was analysed by short-term culture. The supplementation of the medium with 50% FCS and RS resulted in a marked decrease of H-thymidine incorporation into islet DNA similar to that observed with 50% NCS. In contrast, exposure of islets to different batches of HS never resulted in an inhibition of DNA synthesis. Moreover, the 50% concentration of HS 465 caused a significant stimulation of thymidine incorporation.
Dunger
A
A
Institute of Diabetes Gerhardt Katsch, Karlsburg, Germany.
Klöting
I
I
Besch
W
W
Hahn
H J
HJ
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D013045:Species Specificity; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D001798:Blood Proteins; D007328:Insulin; D014316:Tritium; D004247:DNA; D013936:Thymidine