title: Novel model for study of human sperm chemotaxis.
abstract: OBJECTIVE
  To develop and test an adequate method for the study of sperm chemotaxis.
  
  
  METHODS
  A model based on a double-chamber design and a matrix of 0.5% hyaluronic acid was used to test the response of human sperm to chemotactic signals from follicular fluid (FF).
  
  
  RESULTS
  A plateau of migration was reached at 10 minutes of incubation; under this condition, 70% of sperm migrated toward chambers with FF, less than 20% to the opposite side, and the rest remained at the site of application. Intra-assay and interassay coefficients of variation using pools of FFs were 17.2% and 23%, respectively.
  
  
  CONCLUSIONS
  These data confirm previous findings and support the use of this novel model for the study of chemotaxis on human gametes.
authors.affiliation: Instituto Nacional de Perinatología, México City, México.
authors.forename: C
authors.initials: C
authors.lastname: Villanueva-Díaz
authors.forename: J
authors.initials: J
authors.lastname: Arias-Martínez
authors.forename: H
authors.initials: H
authors.lastname: Bustos-López
authors.forename: F
authors.initials: F
authors.lastname: Vadillo-Ortega
mesh_terms: D002633:Chemotaxis; D004958:Estradiol / Q000032:analysis; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D007700:Kinetics; D008297:Male; D011374:Progesterone / Q000032:analysis; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D011374:Progesterone; D004958:Estradiol