Interleukin 1 beta improves survival following cecal ligation and puncture.
Despite antibiotic therapy intra-abdominal sepsis following major surgery is a significant cause of mortality. We sought to determine if interleukin-1 beta (IL-1) could improve survival in a murine model of intra-abdominal infection. Groups of 10 BDF1 mice received a single subcutaneous (sc) injection of recombinant human IL-1 beta 24 hr prior to cecal ligation and puncture (CLP) and were assessed twice daily for survival. Mice that received a single injection of IL-1 beta 24 hr prior to CLP had a dose-dependent improval in survival compared to controls. The beneficial effect of IL-1 treatment may have been related to its ability to stimulate myelopiesis. The addition of indomethacin, in an effort to limit possible toxicity of IL-1, did not further improve survival. Appropriate timing of specific immunomodulators may provide an additional strategy for the treatment of infections.
O'Reilly
M
M
Department of Anesthesia, University of Vermont, Burlington 05401.
Silver
G M
GM
Davis
J H
JH
Gamelli
R L
RL
Hebert
J C
JC
D000005:Abdomen; D000818:Animals; D002432:Cecum; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000473:pathology; D007239:Infections / Q000097:blood / Q000209:etiology / Q000401:mortality*; D007375:Interleukin-1 / Q000494:pharmacology*; D007958:Leukocyte Count; D008026:Ligation; D008264:Macrophages / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011677:Punctures; D011994:Recombinant Proteins
D007375:Interleukin-1; D011994:Recombinant Proteins