Receptor for pre-S1(21-47) component of hepatitis B virus on the liver cell: role in virus cell interaction.
Attachment of hepatitis B virus to a hepatoblastoma cell line (HepG2) was examined using a synthetic peptide corresponding to the pre-S1 (21-47) region of the envelope protein. Scatchard analysis revealed a single class binding site of Kd 104 +/- 27 nM/l and 5.4 +/- 1.2 x 10(5) sites per cell. Competition of HBV with pre-S1 peptides was dose dependent, and demonstrated it as the dominant binding site. In view of the suggested sequence homology between the peptide and IgA, cross-competition studies were carried out. The results indicate no direct role of IgA receptor in HBV binding. The receptor for the pre-S1 peptide was identified as a single major peptide of molecular weight 31 kD using in-situ ligand receptor crosslinking.
Dash
S
S
Department of Pathology, All India Institute of Medical Sciences, New Delhi.
Rao
K V
KV
Panda
S K
SK
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000378:metabolism*; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D008099:Liver / Q000382:microbiology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011991:Receptors, Virus / Q000302:isolation & purification / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000382:microbiology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism
D006514:Hepatitis B Surface Antigens; D007070:Immunoglobulin A; D010446:Peptide Fragments; D011498:Protein Precursors; D011991:Receptors, Virus; D014759:Viral Envelope Proteins; C050806:presurface protein 1, hepatitis B surface antigen