title: Purification of the MglC/E membrane proteins of the binding protein-dependent galactose transport system of Salmonella typhimurium.
abstract: The high affinity galactose transport system of Salmonella typhimurium consists of four proteins, a periplasmic galactose binding protein (the MglB protein), and three inner membrane-associated proteins, the MglA, MglC and MglE proteins. We purified the MglC/E proteins from an MglC/E hyperproducing strain after solubilisation of inclusion bodies in guanidine hydrochloride followed by renaturation in a detergent-containing buffer and affinity chromatography on a MglB-Sepharose column. The MglC/E proteins are devoid of ATPase activity and they complement an extract from a strain carrying a plasmid with the mglA gene for reconstitution of the MglB-dependent galactose transport in proteoliposomes.
authors.affiliation: Biochimie Génétique, Institut Jacques Monod, Université Paris, France.
authors.forename: G
authors.initials: G
authors.lastname: Richarme
authors.forename: M
authors.initials: M
authors.lastname: Kohiyama
mesh_terms: D001426:Bacterial Proteins; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000302:isolation & purification* / Q000378:metabolism; D009004:Monosaccharide Transport Proteins; D011510:Proteolipids / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; C075401:MglC protein, bacteria; C075402:MglE protein, Salmonella typhimurium; D009004:Monosaccharide Transport Proteins; D011510:Proteolipids; D011994:Recombinant Proteins; C020485:proteoliposomes; D005690:Galactose