K-current mediation of prolactin-induced proliferation of malignant (Nb2) lymphocytes.
The effects of different concentrations of various K-current blockers on prolactin-induced proliferation and membrane K-currents in malignant lymphocytes (Nb2 cells) were investigated. Membrane currents were measured with the whole cell patch-clamp technique, and lymphocyte density was quantified by both spectrophotometric and conventional methods. K-current blockers tested (quinidine, 4-aminopyridine, barium, and tetraethylammonium) exhibited similar rank order potency for K-current block and inhibition of prolactin-induced proliferation of malignant lymphocytes. Because Nb2 cells proliferate independently of a transmembrane Ca-influx, these results suggest that K-currents per se rather than K-current modulation of Ca-influx is an essential event for lymphocyte proliferation.
Wang
Y F
YF
Division of Biomedical Sciences, University of California, Riverside 92521-0121.
Jia
H
H
Walker
A M
AM
Cukierman
S
S
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D008214:Lymphocytes / Q000473:pathology* / Q000502:physiology; D008223:Lymphoma / Q000473:pathology / Q000503:physiopathology; D015221:Potassium Channels / Q000378:metabolism / Q000502:physiology*; D011388:Prolactin / Q000494:pharmacology*; D011802:Quinidine / Q000494:pharmacology; D051381:Rats; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000473:pathology
D015221:Potassium Channels; D013757:Tetraethylammonium Compounds; D001464:Barium; D019789:Tetraethylammonium; D011388:Prolactin; D015761:4-Aminopyridine; D011802:Quinidine