title: Adenovirus capsid proteins interact with HSP70 proteins after penetration in human or rodent cells.
abstract: Soon after penetration of adenovirus serotype 2 in BHK-21 and HeLa cells, HSP70 and HSC70 proteins become associated with the viral capsid. By analysis with a polyclonal antibody derived from a fusion protein containing the C-terminal domain, 290 amino acids of HSP70, and using both immunological methods and infected cells fractionation we observed that a significant amount of HSP70 proteins moved to the nucleus and colocalized with the adenovirus particles. HSP70 proteins of infected cells were isolated as a complex cross-linked with intracytoplasmic adenovirus type 2. By coprecipitation, using a polyclonal-specific antiserum derived from the fusion protein, or two different monoclonal-specific antisera, we showed that HSP70 and HSC70 proteins were associated with hexon, the major adenovirus capsid protein.
authors.affiliation: Laboratoire de Virologie Moléculaire, INSERM U.233, Lille, France.
authors.forename: J
authors.initials: J
authors.lastname: Niewiarowska
authors.forename: J C
authors.initials: JC
authors.lastname: D'Halluin
authors.forename: M T
authors.initials: MT
authors.lastname: Belin
mesh_terms: D000256:Adenoviridae; D000818:Animals; D000911:Antibodies, Monoclonal; D002213:Capsid / Q000378:metabolism*; D036022:Capsid Proteins; D002460:Cell Line / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D011233:Precipitin Tests; D014771:Virion / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D006360:Heat-Shock Proteins; C018315:hexon capsid protein, Adenovirus