[Regulation of the phospholipid turnover rate and protein kinase C activity as a necessary stage in the realization of the growth-inhibiting effect of dexamethasone on hepatoma 22 cells].
The role of protein kinase C and phospholipid turnover in the realization of the cytostatic effect of dexamethasone on hormone-sensitive cells of mouse hepatoma 22 has been studied. It was found that dexamethasone added to hepatoma cells induces a rapid (within 30 min) inhibition of the protein kinase C activity with a simultaneous decrease of the 32P incorporation into the major phospholipids (phosphatidylglycerol, phosphatidylcholine, and phosphoinositides). Analysis of correlation between the protein kinase C activity and phospholipid turnover rate revealed that phosphatidylglycerol and phosphatidylcholine synthesis is under the positive control of protein kinase C, whereas that of phosphoinositides is not controlled by the enzyme. A proportional decrease in the rates of metabolism of all the three major phospholipids after addition of the hormone to hepatoma cells suggests that inhibition of phospholipid turnover is one of the primary manifestations of the dexamethasone effect. The hormone-induced decrease in the protein kinase C activity may be regarded as being due to these changes.
Krasil'nikov
M A
MA
Bezrukov
V M
VM
Shatskaia
V A
VA
D000818:Animals; D002746:Chlorpromazine / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D003907:Dexamethasone / Q000494:pharmacology*; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D010743:Phospholipids; D003907:Dexamethasone; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002746:Chlorpromazine
Reguliatsiia skorosti obrashcheniia fosfolipidov i aktivnosti proteinkinazy C kak neobkhodimyĭ étap v realizatsii rost-ingibiruiushchego deĭstviia deksametazona.