A sequence element associated with the Plasmodium falciparum KAHRP gene is the site of developmentally regulated protein-DNA interactions.
The Plasmodium falciparum gene encoding the knob associated histidine-rich protein (KAHRP) is shown to be transcriptionally regulated during its expression in the intraerythrocytic cycle as demonstrated by stage specific nuclear run-on analysis. The genomic organization of the KAHRP gene was determined and the structural basis for the stage specific transcription investigated. A sequence motif with two-fold symmetry was found 160 bp upstream of the RNA initiation site. This sequence element interacts with parasite derived nuclear extracts in a stage specific manner that correlates with the transcriptional activity of the KAHRP gene. These studies suggest a functional role for this structural element in the developmental regulation of a P. falciparum erythrocytic gene.
Lanzer
M
M
DeWitt Wallace Research Laboratory, Division of Molecular Biology, Sloan-Kettering Institute, New York, NY 10021.
de Bruin
D
D
Ravetch
J V
JV
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D005786:Gene Expression Regulation / Q000235:genetics*; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D010963:Plasmodium falciparum / Q000235:genetics* / Q000254:growth & development; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
D010455:Peptides; D015800:Protozoan Proteins; C042968:knob protein, Plasmodium falciparum