title: Quantitative analysis of platelet-activating factor in rat brain.
abstract: Age-related decrease of the platelet-activating factor (PAF) content in rat brain was shown by a convenient method consisting of solid extraction of lipids with a Sep-Pak C-18 cartridge, lipid separation by HPLC and bioassay on rabbit platelets. This method was sufficiently sensitive to allow measurement of PAF in a single brain, and the recovery of PAF was quite high throughout the procedure.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tokumura
authors.forename: T
authors.initials: T
authors.lastname: Yotsumoto
authors.forename: T
authors.initials: T
authors.lastname: Hoshikawa
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Tsukatani
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D010972:Platelet Activating Factor / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010972:Platelet Activating Factor