[Experimental studies on the effects of alpha-tocopherol in small intestinal ischemia and reperfusion injury].
In the present study, we quantified the biochemical [thiobarbituric acid (TBA) reactants, superoxide dismutase (SOD) and vitamin E] and histologic changes in the small intestinal tissue after ischemia and/or reperfusion. Sixty-seven Wistar rats were divided into 7 groups; N group: control, A-I group: 30 min. ischemia, A-II group: 120 min. ischemia, B-I group: Declamp after 30 min. ischemia, B-II group: 30 min. reperfusion after 30 min. ischemia, B-III group: 30 min. reperfusion after 120 min. ischemia, E group: vitamin E administration 30 min. reperfusion after 30 min. ischemia. The levels of TBA reactants were significantly different between A-II and B-II, B-II and E (all p less than 0.01). For SOD, there were significant differences between N and B-I (p less than 0.01), N and E (p less than 0.05). For vitamin E, there were significant differences between A-I and B-I, A-I and B-I, B-II and E (all p less than 0.01). Histologic changes showed that the grade of tissue injury was more severe in B-I and B-II than in A-I, and was less in E than in B-II. It is suggested that vitamin E protected cells from injury due to oxygen free radicals.
Hanai
G
G
Department of Surgery, Fujita Gakuen Health University School of Medicine, Toyoake, Japan.
Kimura
T
T
Shiraishi
T
T
Sano
M
M
Matsumoto
S
S
Funabiki
T
T
Yoshizaki
S
S
D000818:Animals; D005609:Free Radicals; D007421:Intestine, Small / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D007511:Ischemia / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D013858:Thiobarbiturates / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*
D005609:Free Radicals; D008054:Lipid Peroxides; D013858:Thiobarbiturates; D014810:Vitamin E; D013482:Superoxide Dismutase; C029684:thiobarbituric acid; D010100:Oxygen