Identification of non heparin-binding endothelial cell growth factor from rat myofibroblasts.
Myofibroblasts (Mfs) from rat fat tissues produced a potent endothelial cell growth factor (Mf-ECGF). The growth factor activity found in the conditioned media from primary cultures of Mfs, was labile to heat (80 degrees C for 10 min) and proteinase (trypsin), and did not bind to heparin in the presence of 0.2 M NaCl. Mf-ECGF was partially purified 4760-fold with a recovery of 25% from serum-free conditioned media by sequential carboxymethyl (CM) ion-exchange column chromatography and gel filtration. This Mf-ECGF activity was recovered from the 40 kD region of a non-reducing SDS-PAGE, and from the pH region between 6.5 and 7 of isoelectric focusing, with recoveries of 20% and 65%, respectively. These results indicated that a major portion of ECGF activity in the conditioned media was clearly distinct from other well-known endothelial cell growth factors including fibroblast growth factors (FGFs).
Sato
N
N
Department of Biochemistry, National Defense Medical College, Tokorozawa, Japan.
Tsuruoka
N
N
Yamamoto
M
M
Nishihara
T
T
Goto
T
T
D000273:Adipose Tissue / Q000098:blood supply; D000302:Adrenal Cortex / Q000098:blood supply; D000818:Animals; D002196:Capillaries; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D016228:Endothelial Growth Factors / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D005347:Fibroblasts / Q000502:physiology; D006493:Heparin / Q000378:metabolism*; D007525:Isoelectric Focusing; D008297:Male; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology*; D011485:Protein Binding; D051381:Rats
D016228:Endothelial Growth Factors; D006493:Heparin