title: Barley (1----3,1----4)-beta-glucanase isoenzyme EI gene expression is mediated by auxin and gibberellic acid.
abstract: Treatment of young barley leaves with indole acetic acid (IAA) or gibberellic acid (GA3) results in a dramatic increase in levels of (1----3,1----4)-beta-glucanase isoenzyme EI transcripts. In young roots of comparable age, levels of isoenzyme EI mRNA are high; IAA inhibits expression while GA3 has no effect on mRNA levels. The addition of both abscisic acid and GA3 to leaves, roots and aleurone layers leads to higher levels of (1----3,1----4)-beta-glucanase isoenzyme EI mRNA than is found with Ga3 alone. Little or no expression of (1----3,1----4)-beta-glucanase isoenzyme EII is detected in vegetative tissues, but in isolated aleurone layers GA3 enhances levels of isoenzyme EII transcripts, as does IAA. Thus, the two barley (1----3,1----4)-beta-glucanase genes respond quite differently to phytohormone treatment, depending on the tissue and its stage of development.
authors.affiliation: Department of Biochemistry, La Trobe University, Bundoora, Victoria, Australia.
authors.forename: N
authors.initials: N
authors.lastname: Slakeski
authors.forename: G B
authors.initials: GB
authors.lastname: Fincher
mesh_terms: D015152:Blotting, Northern; D015971:Gene Expression Regulation, Enzymologic; D005875:Gibberellins / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D001467:Hordeum / Q000033:anatomy & histology / Q000201:enzymology / Q000235:genetics*; D007210:Indoleacetic Acids / Q000502:physiology; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology*
chemical_list: D005875:Gibberellins; D007210:Indoleacetic Acids; D007527:Isoenzymes; D010937:Plant Growth Regulators; C007842:gibberellic acid; D006026:Glycoside Hydrolases; C028020:licheninase