title: The maintenance of free-living amoebae by cryopreservation.
abstract: We have successfully cryopreserved free-living amoebae in order to maintain them feasibly under the conditions in our laboratory. The viability of trophozoites was higher when frozen by slow cooling (overall 0.7 degree C/min) than by fast cooling (overall 1.3 degrees C/min). Glycerol and dimethylsulfoxide at the final concentration of 7.5% each was used for cryopreservation of free-living amoebae trophozoites. The survival rate was 2-39% after storage in the liquid nitrogen for 60 days. Gross cultural or morphological changes were not noted in trophozoites thawed from frozen suspensions.
authors.affiliation: Department of Parasitology, College of Medicine, Yonsei University, Seoul, Korea.
authors.forename: S A
authors.initials: SA
authors.lastname: Seo
authors.forename: T S
authors.initials: TS
authors.lastname: Yong
authors.forename: K
authors.initials: K
authors.lastname: Im
mesh_terms: D000656:Amoeba; D000818:Animals; D015925:Cryopreservation / Q000379:methods