Microheterogeneity of apolipoprotein D as revealed by electroblotting following isoelectric focusing in Immobiline DryPlates.
The microheterogeneity of apolipoprotein D was examined by a procedure involving, in sequences: (i) electrophoresis in an immobilized pH 4-7 gradient in an Immobiline DryPlate-polyacrylamide gel supplemented with Ampholine pH 5-7, (ii) covering of the gel with sodium dodecyl sulfate-containing agarose, (iii) electroblotting onto a polyvinylidene difluoride membrane and (iv) immunological identification. Seven isoforms were obtained with partially purified apolipoprotein D. Using this technique the apparent pI values at 15 degrees C for the isoforms were 4.57, 4.67, 4.78, 4.83 and 5.95, 6.06 and 6.19 (SD +/- 0.05 for all). Direct staining of the Immobiline DryPlate could not reveal the isoforms of partially purified apolipoprotein D.
Holmquist
L
L
Division of Medical Chemistry, National Institute of Occupational Health, Solna, Sweden.
D000178:Acrylamides; D000180:Acrylic Resins; D001053:Apolipoproteins / Q000032:analysis*; D053399:Apolipoproteins D; D005782:Gels; D015151:Immunoblotting; D007525:Isoelectric Focusing
D000178:Acrylamides; D000180:Acrylic Resins; D001053:Apolipoproteins; D053399:Apolipoproteins D; D005782:Gels; C016680:polyacrylamide gels; C059413:Immobiline