Regulation of graft-versus-host-reaction by Mlsa-reactive donor T cells.
Injection of A/J splenocytes (H-2Dd, Mlsc) into unirradiated (A/J x CBA)F1 (BAF1) host mice (H-2Dd/k, Mlsd) results in an acute suppressive graft-vs.-host reaction (GVHR), characterized by immune dysfunction and appreciable donor cell engraftment; injection of the CBA/J parent (H-2Dk, Mlsd), which recognizes no Mls disparity in the host, results in little or no GVHR. Furthermore, the Mlsa-reactive V beta 6 and V beta 8.1 T cell subsets in A/J T cells expand significantly in the GVHR host. Finally, depletion of V beta 6+ and V beta 8.1+ from the A/J population abrogates the proliferative response to BAF1 in vitro and the development of GVHR in vivo. Thus, the response to Mls determinants can contribute to the generation of a GVHR.
Muluk
S C
SC
Experimental Immunology Branch, NCI, NIH, Bethesda 20892.
Hakim
F T
FT
Shearer
G M
GM
D000818:Animals; D006087:Graft vs Host Reaction; D008212:Lymphocyte Depletion; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D016747:Minor Lymphocyte Stimulatory Antigens / Q000502:physiology*; D016176:T-Lymphocyte Subsets / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology*
D016747:Minor Lymphocyte Stimulatory Antigens