Molecular basis for novel root phenotypes induced by Agrobacterium rhizogenes A4 on cucumber.
We have used the wild-type Agrobacterium rhizogenes strain A4 to induce roots on cucumber stem explants. Cultures of transformed roots obtained that were capable of hormone-autonomous growth could be grouped in three phenotypic classes. Of particular interest were extremely thick roots of a type not previously described. Characterization of the transferred DNA and of the expression of the corresponding genes allowed us to determine that the genes rolABC of the TL region of the Ri plasmid are sufficient to induce thin roots similar to those observed in other species, while the aux genes of the TR region are sufficient to induce thick roots. Among clones bearing the aux genes, there was a correlation between level of expression of aux2 and root phenotype.
Amselem
J
J
Laboratoire de Biologie Cellulaire, INRA-Centre de Versailles, France.
Tepfer
M
M
D002455:Cell Division; D002461:Cell Line, Transformed; D005798:Genes, Bacterial; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D009924:Organ Culture Techniques; D010641:Phenotype; D010944:Plants / Q000235:genetics* / Q000382:microbiology; D010957:Plasmids; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics / Q000502:physiology*
D015345:Oligonucleotide Probes