Automated latex nephelometric immunoassay of theophylline in human serum.
A latex test was adapted for the nephelometric quantitation of theophylline in human serum. The assay is fully automated on the Behring Nephelometer Analyser with a sampling rate of 150 samples per hour. There is no interference from bilirubin (up to 340 mumol/l), haemoglobin (up to 7000 mg/l), Intralipid (up to 5 g/l), or rheumatoid factor (up to 1100 x 10(3) IU/l). The theophylline standard curve extends from 1.25 to 40 mg/l. The coefficient of variation ranged from 2.4 to 7.4%. The correlation coefficient between the latex immunoassay and the TDx theophylline procedure was 0.988, calculated from the assay of 74 samples.
Borque
L
L
Laboratorio de Bioquímica, Hospital San Millan, Logroño, Spain.
Rus
A
A
Maside
C
C
del Cura
J
J
D006801:Humans; D007118:Immunoassay / Q000379:methods; D009391:Nephelometry and Turbidimetry / Q000379:methods; D013806:Theophylline / Q000097:blood*
D013806:Theophylline