Characterization of a new picorna-like virus, himetobi P virus, in planthoppers.
Picorna-like virus particles, 29 nm in diameter, were purified from apparently healthy Laodelphax striatellus Fallen. The virus particles had a buoyant density of 1.352 g/ml in CsCl and a sedimentation coefficient of 161 s. The virus capsid proteins consisted of three major polypeptides of M(r)s 36,500, 33,000 and 28,000, and three minor polypeptides. The virus contained a major ssRNA of M(r) 2.8 x 10(6) and was also frequently associated with a minor dsRNA of M(r) 4 x 10(6). The 3' end of the ssRNA had a poly(A) tract of about 60 adenine residues. The virus has been provisionally named himetobi P virus.
Toriyama
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National Institute of Agro-Environmental Sciences, Ibaraki, Japan.
Guy
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PL
Fuji
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Takahashi
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D000818:Animals; D001483:Base Sequence; D004199:Disease Vectors; D007303:Insect Vectors / Q000382:microbiology*; D007313:Insecta / Q000382:microbiology*; D008969:Molecular Sequence Data; D012275:Oryza / Q000469:parasitology; D010849:Picornaviridae / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure; D012330:RNA, Double-Stranded / Q000737:chemistry; D014764:Viral Proteins / Q000032:analysis
D012330:RNA, Double-Stranded; D014764:Viral Proteins