Avidin-binding to peripheral blood B lymphocytes and monocytes.
Avidin-coated magnetic beads bind peripheral blood B lymphocytes and monocytes. This unwanted reactivity is not due to the membrane expression of avidin target molecules since beads coated with a biotin-binding analogue are non-reactive and binding occurs even when cellular carbohydrate-binding sites are not active, in the absence of Mg2+ and Ca2+ cations, or when they are blocked by a alpha-D-glucose or alpha-D-mannose in presence of Ca2+ and Mg2+. The non-polar residues of avidin appear not to be engaged in a hydrophobic bond with the membrane molecule since suroptimal quantities of serum albumin do not prevent the avidin binding. It is suggested that ionic interactions explain the binding of avidin-coated beads to B lymphocytes and monocytes and that these can be inhibited with high molecular weight serum molecules or with 0.4 M NaCl.
Andreoni
C
C
Immunotech S.A., Marseilles, France.
Delaage
M
M
D001360:Avidin / Q000737:chemistry / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D007477:Ions; D008280:Magnetics; D009000:Monocytes / Q000378:metabolism*; D009994:Osmolar Concentration
D007477:Ions; D001360:Avidin