A YIGSR-containing novel mutein without the detrimental effect of human TNF-alpha of enhancing experimental pulmonary metastasis.
The injection of B16F10 melanoma cells with recombinant human tumor necrosis factor alpha (TNF-alpha) into the tail vein of C57BL/6 mice resulted in 2- to 25-fold more metastatic foci in the lungs than the injection of tumor cells alone. Clearly, TNF-alpha significantly enhanced experimental tumor metastasis. Furthermore, it enhanced the metastasis of Lewis lung carcinoma cells. In contrast, a mutein of TNF-alpha, designated as F4236, having the cell-adhesive sequence (Tyr-Ile-Gly-Ser-Arg) at the N-terminus of the TNF molecule did not enhance metastasis, but rather exhibited similar antitumor activity to wild-type TNF-alpha in fibrosarcoma-bearing mice.
Miyata
K
K
Biochemistry Research Laboratory, Central Research Institute, Shiga, Japan.
Kato
M
M
Shikama
H
H
Nishimura
K
K
Sakae
N
N
Kawagoe
K
K
Nishikawa
T
T
Kuroda
K
K
Yamaguchi
K
K
Aoyama
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D006801:Humans; D008175:Lung Neoplasms / Q000139:chemically induced / Q000556:secondary*; D008546:Melanoma, Experimental / Q000139:chemically induced / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009154:Mutation; D009368:Neoplasm Transplantation; D010455:Peptides / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000009:adverse effects* / Q000235:genetics / Q000494:pharmacology
D010455:Peptides; D014409:Tumor Necrosis Factor-alpha