The phosphate groups of the high mobility group like protein P1 strengthens its affinity for DNA.
PCA soluble proteins isolated from rat liver and proliferating HeLa interphase cells were subjected to chromatography on columns containing immobilized s.s and d.s. DNA. P1 from rat liver was eluted from s.s. and d.s. DNA between 0.20 and 0.45 M NaCl, while dephosphorylated P1 was not retained by s.s. and d.s. DNA columns at 0.25 M, suggesting that phosphate groups enhance the affinity of P1 for DNA. P1 from proliferating HeLa interphase cells exhibit increased affinity for d.s. as well as s.s. DNA when compared to rat liver P1. The higher extent of phosphorylation in proliferating cells supports the finding that phosphate enhances rather than reduces the affinity of P1 for DNA.
Ostvold
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AC
Neurochemical Laboratory, University of Oslo, Norway.
Hullstein
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I
Laland
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SG
D000818:Animals; D001345:Autoradiography; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded; D006367:HeLa Cells; D006609:High Mobility Group Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D051381:Rats
D004277:DNA, Single-Stranded; D006609:High Mobility Group Proteins; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D004247:DNA