Rapid determination of amino acids by high-performance liquid chromatography: release of amino acids by perfused rat liver.
Perfused rat liver can be considered as one of the most suitable ex vivo models for studies of liver metabolism. To assess the possible effect of L-carnitine and some of its acyl esters on proteolysis in the rat liver, the amino acid derivatization and high-performance liquid chromatographic separation of Tapuhi et al. [Anal. Biochem., 115 (1981) 123] was modified.
Zezza
F
F
Department of Biochemistry, Research and Development, Sigma-tau SpA, Rome, Italy.
Kerner
J
J
Pascale
M R
MR
Giannini
R
R
Martelli
E A
EA
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002331:Carnitine / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D002331:Carnitine