[Type and incidence of cytogenetic abnormalities in unfertilized or uncleaved human oocytes within the scope of in vitro fertilization].
Human oocytes remaining unfertilized or uncleaved during in vitro fertilization (IVF) yield valuable information on the kind and frequency of numerical and structural chromosome aberrations after their fixation. This enables us to assess the maternal contribution to perturbations in the course of fertilization and early embryonic development. Therefore, 566 unfertilized or uncleaved oocytes from our IVF programme were processed for cytogenetic examination. 223 gametes were not analyzable. 260 oocytes were completely karyotyped and chromosome counts could be established for 83 cells. 243 eggs had the normal haploid chromosome complement whereas 100 cells (29.2%) were abnormal. In detail, we found the following aberrations: hypohaploidy = 10.2%, hyperhaploidy = 4.7%, diploidy = 9.0%, tetraploidy = 1.2%, structural anomalies = 2.6%, prematurely condensed sperm chromosomes = 3.2%. Six cells had to be considered in two of these categories. Our results and comparisons with other publications imply that the total rate of anomaies in human oocytes from stimulated cycles amounts to 20 to 30%. This high frequency might be one of the limiting factors for the success rate of IVF.
Rosenbusch
B
B
Abteilung Frauenheilkunde und Geburtshilfe, Universität Ulm.
Sterzik
K
K
Sasse
V
V
Djalali
M
M
Lauritzen
C
C
D000328:Adult; D002869:Chromosome Aberrations / Q000235:genetics*; D002970:Cleavage Stage, Ovum / Q000648:ultrastructure*; D005260:Female; D005307:Fertilization in Vitro; D006238:Haploidy; D006801:Humans; D009865:Oocytes / Q000648:ultrastructure*
Art und Häufigkeit zytogenetischer Anomalien in unbefruchtet oder ungeteilt gebliebenen menschlichen Eizellen im Rahmen der In-vitro-Fertilsation.