Differential inhibition of fat intake in two strains of rat by the peptide enterostatin.
The effect of enterostatin, the amino-terminal pentapeptide of pancreatic procolipase, on food intake was investigated in two strains of rat, the dietary fat-sensitive Osborne-Mendel (OM) rat and the dietary fat-resistant S5B/Pl rat. After an overnight fast, enterostatin inhibited intake of high-fat (HF) but not low-fat (LF) diets in OM rats, but had no effect in S5B/Pl rats. When fed a macronutrient three-choice diet, OM rats selected fat preferentially, whereas S5B/Pl rats selected carbohydrate. Enterostatin specifically inhibited the intake of fat in OM rats but not S5B/Pl rats fed a three-choice macronutrient diet. The activity of pancreatic colipase was increased in S5B/Pl rats on both HF and LF diets compared with OM rats. Pancreatic colipase activities were negatively related to the voluntary intake of fat on three-choice macronutrient diets. The data support the hypothesis that enterostatin may control the intake of dietary fat.
Okada
S
S
Pennington Biomedical Research Center, Baton Rouge, Louisiana 70808.
York
D A
DA
Bray
G A
GA
Mei
J
J
Erlanson-Albertsson
C
C
D000818:Animals; D003089:Colipases / Q000378:metabolism / Q000494:pharmacology*; D004041:Dietary Fats; D004435:Eating / Q000187:drug effects*; D004792:Enzyme Precursors; D005215:Fasting; D005518:Food Preferences; D020022:Genetic Predisposition to Disease; D008297:Male; D009765:Obesity / Q000235:genetics; D010179:Pancreas / Q000201:enzymology; D011498:Protein Precursors / Q000494:pharmacology*; D051381:Rats; D011922:Rats, Mutant Strains
D003089:Colipases; D004041:Dietary Fats; D004792:Enzyme Precursors; D011498:Protein Precursors; C037107:procolipase