Undecalcified temporal bone morphology: a methodology useful for gross to fine observation and three-dimensional reconstruction.
Using a novel method of cutting undecalcified temporal bone specimens, quantitative structural analysis in the human and the Japanese monkey was undertaken. One millimeter thick serial slices made from unembedded temporal bones retained fine structure. Therefore, gross to fine observation could be performed systematically at the macroscopic, light, scanning, and transmission electron microscopic levels. The entire temporal bone three-dimensional reconstruction was completed from embedded sections; consequently, the volume of the tubotympanum and air cell system could be calculated. Available methods by embedding, tungsten carbide sectioning, grinding, and microwave irradiation for decalcification were also examined. These morphologic studies suggest that these novel methods offer timesaving advantages over any presently available techniques, and allow for elucidation of temporal bone morphology with only a few specimens.
Fujiyoshi
T
T
Department of Otolaryngology, Medical College of Oita, Japan.
Mogi
G
G
Watanabe
T
T
Matsushita
F
F
D000818:Animals; D003648:Decalcification Technique; D005260:Female; D016591:Histocytological Preparation Techniques / Q000295:instrumentation*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008251:Macaca / Q000033:anatomy & histology; D008297:Male; D008854:Microscopy, Electron; D008867:Microtomy; D013701:Temporal Bone / Q000033:anatomy & histology* / Q000648:ultrastructure