Infection of Macaca nemestrina by human immunodeficiency virus type-1.
After observations that Macaca nemestrina were exceptionally susceptible to simian immunodeficiency virus and human immunodeficiency virus type-2 (HIV-2), studies of HIV-1 replication were initiated. Several strains of HIV-1, including a recent patient isolate, replicated in vitro in peripheral blood mononuclear cells (PBMCs) and in CD4-positive M. nemestrina lymphocytes in a CD4-dependent fashion. Eight animals were subsequently inoculated with either cell-associated or cell-free suspensions of HIV-1. All animals had HIV-1 isolated by cocultivation, had HIV-1 DNA in their PBMCs as shown by polymerase chain reaction, and experienced sustained seroconversion to a broad spectrum of HIV-1 proteins. Macaca nemestrina is an animal model of HIV-1 infections that provides opportunities for evaluating the pathogenesis of acute HIV-1 replication and candidate vaccines and therapies.
Agy
M B
MB
Regional Primate Research Center, University of Washington, Seattle, WA 98195.
Frumkin
L R
LR
Corey
L
L
Coombs
R W
RW
Wolinsky
S M
SM
Koehler
J
J
Morton
W R
WR
Katze
M G
MG
D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000502:physiology; D003545:Cysteine / Q000378:metabolism; D016208:Databases, Factual; D015750:Genes, gag; D015658:HIV Infections / Q000503:physiopathology*; D006679:HIV Seropositivity; D015497:HIV-1 / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology; D008254:Macaca nemestrina / Q000382:microbiology*; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D014779:Virus Replication
D015704:CD4 Antigens; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D014764:Viral Proteins; D008715:Methionine; D003545:Cysteine