Auxotrophy of Pseudomonas aeruginosa in cystic fibrosis.
Seventy-four of 403 (18.4%) sputum isolates of Pseudomonas aeruginosa from 49 of 136 (36.0%) adults with cystic fibrosis (CF) were auxotrophic mutants. Two of 11 (18.2%) isolates of P. aeruginosa taken from patients with non-CF bronchiectasis were also auxotrophic. All 99 strains taken from non-bronchiectatic sources were prototrophic. Forty-six of 55 (83.6%) CF auxotrophs required one or more of 36 growth factors tested; the requirements for the remaining 9 isolates were not identified. Methionine was the sole factor required by 17 of 22 (77.3%) isolated which depended on a single factor. We conclude that auxotrophy is a feature of P. aeruginosa infection in cystic fibrosis.
Taylor
R F
RF
Royal Brompton and National Heart Hospital, London, UK.
Hodson
M E
ME
Pitt
T L
TL
D000596:Amino Acids / Q000235:genetics / Q000378:metabolism*; D001120:Arginine / Q000235:genetics / Q000378:metabolism; D001987:Bronchiectasis / Q000382:microbiology; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D008715:Methionine / Q000235:genetics / Q000378:metabolism; D011392:Proline / Q000235:genetics / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013183:Sputum / Q000382:microbiology; D013831:Thiamine / Q000235:genetics / Q000378:metabolism
D000596:Amino Acids; D001120:Arginine; D011392:Proline; D008715:Methionine; D013831:Thiamine