Mitogen-induced expression of the primary response gene cMG1 in a rat intestinal epithelial cell-line (RIE-1).
cMG1 is a primary response gene first identified in a rat intestinal epithelial (RIE-1) cell-line [(1990) Oncogene 5, 1081-1083]. A number of mitogens, including epidermal growth factor (EGF), angiotensin II (AII), serum and insulin rapidly induced 2- to 6-fold increases of cMG1 mRNA in RIE-1 cells, while transforming growth factor-beta caused a small reduction. Cyclic AMP-elevating agents blocked the increase of cMG1 mRNA induced by EGF. The AII-stimulated increase of cMG1 mRNA was blocked by the depletion of protein kinase C, whereas the EGF-stimulated increase was not affected, indicating that protein kinase C-dependent and -independent signalling pathways stimulate cMG1 expression.
Gomperts
M
M
Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Cambridge, UK.
Corps
A N
AN
Pascall
J C
JC
Brown
K D
KD
D016475:3T3 Cells; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D015152:Blotting, Northern; D051817:Butyrate Response Factor 1; D002460:Cell Line; D004268:DNA-Binding Proteins; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D016762:Genes, fos; D017874:Immediate-Early Proteins; D007328:Insulin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D051816:Tristetraprolin; D014407:Tumor Cells, Cultured
D051817:Butyrate Response Factor 1; D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D007328:Insulin; D008934:Mitogens; D011506:Proteins; D012333:RNA, Messenger; D051816:Tristetraprolin; C497553:Zfp36 protein, mouse; C497554:Zfp36 protein, rat; C497555:Zfp36l1 protein, rat; D000804:Angiotensin II; D004815:Epidermal Growth Factor; D011493:Protein Kinase C