Expression of nef, vpu, CA and CD4 during the infection of lymphoid and monocytic cell lines with HIV-1.
The expression of the capsid antigen (CA) and the two regulatory proteins nef and vpu as well as the CD4 cell surface receptor was followed in HIV-infected lymphoid and promonocytic cells. In the lytic phase of infection all three viral proteins were expressed; production of these proteins coincided with the increase of CA antigen and infectious virus in culture supernatants and with prominent cytopathic effects. After selection of persistently infected cells, the number of lymphoid cells expressing detectable levels of nef decreased to zero; the number of cells positive for CA ranged between 40 to 70%. In chronically infected promonocytic cells nef and vpu expression was reduced to undetectable levels, whereas most of the cells accumulated CA intracellularly. Infectious cell free virus and CA in the supernatant of promonocytic cells had low titers. CD4 surface expression declined in all cell lines investigated before cell free virus was detectable.
Schneider
T
T
AIDS-Zentrum am Bundesgesundheitsamt, Robert Koch-Institut, Berlin, Federal Republic of Germany.
Hildebrandt
P
P
Rokos
K
K
Schubert
U
U
Rönspeck
W
W
Grund
C
C
Beck
A
A
Blesken
R
R
Kulins
G
G
Oldenburg
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015704:CD4 Antigens / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D015693:Gene Products, nef / Q000096:biosynthesis* / Q000235:genetics; D016655:HIV Core Protein p24 / Q000096:biosynthesis* / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D054298:Human Immunodeficiency Virus Proteins; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009000:Monocytes / Q000382:microbiology; D013601:T-Lymphocytes / Q000382:microbiology*; D054334:Viral Regulatory and Accessory Proteins / Q000096:biosynthesis* / Q000235:genetics; D014779:Virus Replication; D054311:nef Gene Products, Human Immunodeficiency Virus; D000086082:Viroporin Proteins
D000914:Antibodies, Viral; D015704:CD4 Antigens; D015693:Gene Products, nef; D016655:HIV Core Protein p24; D054298:Human Immunodeficiency Virus Proteins; D054334:Viral Regulatory and Accessory Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus; C514859:vpu protein, Human immunodeficiency virus 1; D000086082:Viroporin Proteins