Characterization of a YAC and cosmid contig containing markers tightly linked to the myotonic dystrophy locus on chromosome 19.
Myotonic dystrophy (DM) is caused by a defect in an unknown gene that maps to 19q13.3, flanked by the tightly linked markers ERCC1 on the proximal side and D19S51 on the distal side. We report the isolation and characterization of overlapping YAC and cosmid clones around D19S51 for the construction of a physical map around this locus. The resulting contig contains the markers D19S51 and D19S62 (another new marker tightly linked to the DM locus) and the distal breakpoint of a radiation hybrid cell line used in the physical mapping of the DM region. We have compared the restriction maps of the YACs and cosmids with that of the genome to investigate the fidelity of these clones.
Buxton
J
J
Department of Anatomy, Charing Cross and Westminster Medical School, London, United Kingdom.
Shelbourne
P
P
Davies
J
J
Jones
C
C
Perryman
M B
MB
Ashizawa
T
T
Butler
R
R
Brook
D
D
Shaw
D
D
de Jong
P
P
D001483:Base Sequence; D015825:Chromosomes, Fungal; D002888:Chromosomes, Human, Pair 19; D003360:Cosmids; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D015723:Gene Library; D008040:Genetic Linkage; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D009223:Myotonic Dystrophy / Q000235:genetics*; D015183:Restriction Mapping
D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA