HSP70-related 65 kDa protein of beet yellows closterovirus is a microtubule-binding protein.
Beet yellows virus (BYV) genome encodes a 65 kDa protein homologous to the HSP70 family of cellular heat-shock proteins (Agranovsky, A.A., Boyko, V.P., Karasev, A.V., Koonin, E.V. and Dolja, V.V. (1991) J. Mol. Biol. 217, 603-610). The respective gene was cloned and expressed in vitro yielding a product of the expected size (p65). This product was found to bind to the purified microtubules with a binding constant of 4 x 10(-7) M. The binding of p65 was stimulated if ATP presented in the translation mixture was hydrolyzed by apyrase. Removal of the short C-terminal domains of alpha- and beta-tubulin by subtilisin digestion abolished the binding, demonstrating its specificity. The possible role of p65 association with microtubules in the movement of virus within and/or between plant cells is proposed.
Karasev
A V
AV
Institute of Microbiology, Academy of Sciences of Russia, Moscow.
Kashina
A S
AS
Gelfand
V I
VI
Dolja
V V
VV
D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D010942:Plant Viruses / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D006360:Heat-Shock Proteins; D008869:Microtubule-Associated Proteins; D014764:Viral Proteins