Characterization of a novel arachidonic acid-derived neutrophil chemoattractant.
The oxidation of arachidonic acid in a superoxide-generating environment results in the formation of a potent chemoattractant that appears to be identical to a chemotactic material generated by hepatocytes when they metabolize alcohol. The product was extracted, chromatographed and characterized by physical methods including GC/MS. The physical properties are consistant with the parent structure: 19-hydroperoxy, 20-hydroxyarachidic acid. This novel saturated 20 carbon product, derived from arachidonic acid by free radical-generating reactions, may play a role in the neutrophilic infiltration observed during the course of acute alcoholic hepatitis.
Roll
F J
FJ
Department of Medicine, University of California, San Francisco 94143.
Perez
H D
HD
Serhan
C N
CN
D000818:Animals; D001095:Arachidonic Acids / Q000097:blood*; D019328:Buthionine Sulfoximine; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004537:Eicosanoic Acids / Q000097:blood* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008163:Luminescent Measurements; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats
D001095:Arachidonic Acids; D002630:Chemotactic Factors; D004537:Eicosanoic Acids; C075623:19-hydroperoxy-20-hydroxyarachidic acid; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine