Regulation of poly(ADP-ribose) polymerase gene expression in mitogen-stimulated human peripheral blood mononuclear cells.
The level of mRNA for poly(ADP-ribose) polymerase in human PBMC increased 8 h after addition of PHA, reaching its maximum (9-fold over the basal level) 3-4 days after the stimulation and decreasing thereafter. mRNA maximum slightly preceded in time the maximal value of DNA synthesis. The half-life of poly(ADP-ribose) polymerase mRNA, which is 1.2 h in quiescent PBMC, increased up to 3.4 h in stimulated PBMC. This PHA-induced stabilization of the mRNA for poly(ADP-ribose) polymerase could account for the accumulation of the transcript in mitogen-treated PBMC.
Menegazzi
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M
Istituto di Chimica Biologica, Università di Verona, Italy.
Carcereri de Prati
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A
Miwa
M
M
Suzuki
H
H
Libonati
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D015152:Blotting, Northern; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000201:enzymology*; D010835:Phytohemagglutinins; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D010835:Phytohemagglutinins; D012333:RNA, Messenger; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases