title: Identification of a rhodopsin photoreceptor in Euglena gracilis.
abstract: Visual pigments are a class of receptor proteins that absorb light and trigger sensory signals. Retinal-containing proteins are used in nature as photoreceptors mainly in animals vision. Mammalian rhodopsin is the best studied example of a light sensor which couples photon absorption to a cascade of biochemical reactions amplifying the input signal. A surprising discovery was to find rhodopsin also in Archaebacteria and in unicellular eukaryotes. On the basis of absorption microspectroscopic measurements and of inhibition experiments on pigment biosynthetic pathways, we have recently suggested that a rhodopsin could be the functional receptor of the visual process in Euglena gracilis, a flagellate which can use light directly to promote photosynthetic reactions, or as an incident flux of information to adjust its swimming orientation. We here report purification and identification of all-trans-retinal by column chromatography, HPLC and GC-MS in E. gracilis; these findings indicate with absolute certainty that rhodopsin is the photoreceptor molecule of this microorganism.
authors.affiliation: Istituto di Biofisica, Pisa, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Gualtieri
authors.forename: P
authors.initials: P
authors.lastname: Pelosi
authors.forename: V
authors.initials: V
authors.lastname: Passarelli
authors.forename: L
authors.initials: L
authors.lastname: Barsanti
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005056:Euglena gracilis / Q000737:chemistry*; D013058:Mass Spectrometry; D012172:Retinaldehyde / Q000737:chemistry / Q000302:isolation & purification*; D012243:Rhodopsin / Q000737:chemistry / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012243:Rhodopsin; D012172:Retinaldehyde