Erythropoietin-induced stimulation of differentiation and proliferation in J2E cells is not mimicked by chemical induction.
The J2E cell line is a novel erythroid cell line that differentiates in response to erythropoietin (Epo), the physiologic stimulus for erythropoiesis. After exposure to Epo, the cells synthesize hemoglobin, and we show here that this process is tightly linked to increases in cellular proliferation and DNA synthesis. The hormone-induced terminal differentiation also results in morphologic alterations and the accumulation of transcripts for alpha, beta maj, and beta min globins. c-myc messenger RNA levels increase rapidly after exposure to Epo and precede the increase in cell division, while c-myb undergoes a transient decrease. Differentiation of J2E cells can also be achieved with sodium butyrate, but, in contrast with Epo, this is associated with a retardation of replication and a sudden decrease in c-myc levels. These results show that, in this system, chemically induced differentiation differs from terminal maturation promoted by Epo and that the processes of proliferation and differentiation in J2E cells can be uncoupled.
Busfield
S J
SJ
Department of Biochemistry, University of Western Australia, Nedlands.
Klinken
S P
SP
D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D015342:DNA Probes; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015672:Erythroid Precursor Cells; D004921:Erythropoietin / Q000494:pharmacology*; D016259:Genes, myc / Q000187:drug effects; D005914:Globins / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D009857:Oncogenes / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D015342:DNA Probes; D006454:Hemoglobins; D012333:RNA, Messenger; D004921:Erythropoietin; D005914:Globins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D013936:Thymidine