[Characterization of LDL receptors in mononuclear cells of healthy probands and patients with familial hypercholesterolemia].
Temperature-dependence, time-course, competition studies and saturation kinetics of the binding of either 123I-LDL or 111In-LDL to peripheral blood mononuclear cells (lymphocytes and monocytes) were determined in normolipemic controls and heterozygous familial hypercholesterolemia (FH) patients. Radioligand binding studies with 123I-LDL and 111In-LDL revealed similar binding characteristics. The maximal binding capacity was significantly reduced in FH patients (p less than 0.01) whereas the binding equilibrium dissociation constant was virtually unchanged as compared to healthy controls.
Banyai
M
M
Wilhelm Auerswald Atheroskleroseforschungsgruppe, Wien.
D001667:Binding, Competitive / Q000502:physiology; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000235:genetics*; D009000:Monocytes / Q000378:metabolism; D011869:Radioligand Assay; D011973:Receptors, LDL / Q000235:genetics*
D011973:Receptors, LDL
Charakterisierung von LDL-Rezeptoren mononukleärer Zellen von gesunden Probanden und von Patienten mit familiärer Hypercholesterinämie.