31P-NMR evaluation of postischemia renal ATP and pH levels after ATP-MgCl2 treatment in rabbits.
Phosphorus-31 (31P) nuclear magnetic resonance (NMR) spectroscopy was used to measure adenosine triphosphate (ATP) concentration and pH in vivo in rabbits subjected to a 40-minute period of unilateral renal ischemia to determine the effect of infusing ATP-magnesium chloride (MgCl2, 100 mumol/kg) versus saline at the initiation of reperfusion. Data were compared initially by analysis of variance and then analyzed further using a general linear model with covariate adjustment. ATP-MgCl2-treated animals did not have higher ATP levels during recovery but did have significantly higher renal blood flow (p less than 0.05), a significantly decreased rate of recovery from acidosis (p less than 0.05), and significantly higher urinary output (p less than 0.01) than saline-treated animals during the recovery period. Therefore, treatment with ATP-MgCl2 improves postischemic functional parameters in this model of moderate injury without functioning as a direct source of ATP or its precursors. These data add support to the emerging concept that intracellular acidosis protects cells from reperfusion injury.
Martin
L F
LF
Department of Surgery, Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey 17033.
Peter
A O
AO
Fehr
D M
DM
Landis
J R
JR
Cotter
J
J
Briggs
R W
RW
D000255:Adenosine Triphosphate / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D005260:Female; D006863:Hydrogen-Ion Concentration; D007511:Ischemia / Q000378:metabolism*; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010758:Phosphorus; D011817:Rabbits; D012079:Renal Circulation; D015424:Reperfusion; D014554:Urination / Q000187:drug effects
D010758:Phosphorus; D000255:Adenosine Triphosphate