A transcriptional analysis of the gene encoding mouse U7 small nuclear RNA.
Expression of the U7 gene, encoding mouse U7 snRNA, following microinjection into Xenopus oocytes is both accurate and efficient, giving rise to mature U7 snRNA and a precursor with an 8-nucleotide (nt) 3' extension. The mouse U7 gene promoter, which is similar to that of the vertebrate major U genes comprising a DSE, a PSE and a 3' box, with the same spatial arrangement, is as efficient as the Xenopus U2 gene promoter in this assay. A deletion analysis of the mouse U7 gene identified sequences downstream from the 3' box, within the region (nt +74 to +196), which seem to have a negative regulatory effect upon the frequency of transcription initiation and are also required for accurate 3' end formation. Sequences in the nt -1699 to -431 region also seemed to have a negative effect on the level of transcription. In addition, sequences upstream from the PSE, within the nt -65 to -421 region, are necessary for accurate and efficient synthesis of mature U7 snRNA. Finally, the mouse U7 snRNA may not form a functional snRNP in Xenopus oocytes due to defective snRNP assembly and/or nuclear import.
Phillips
S C
SC
Department of Biochemistry, University of Liverpool, UK.
Turner
P C
PC
D000818:Animals; D001483:Base Sequence; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D012342:RNA, Small Nuclear / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*; D014981:Xenopus / Q000235:genetics
D012342:RNA, Small Nuclear