Effects of organic acids on the edema of the stria vascularis induced by furosemide.
Furosemide is a loop diuretic which is ototoxic. Investigations have shown the stria vascularis to be the target tissue of this ototoxic drug. The purpose of the present study was to investigate the effects of furosemide on the stria vascularis in chinchillas, in controls and in animals pretreated with the above organic acids. Control animals were injected with 0.5 ml alkalinized saline followed by furosemide IV 30 min later. Experimental animals received probenecid, penicillin or sodium salicylate IV. Thirty minutes later, furosemide was injected in the same dose as in the controls. The basal turn of the stria vascularis was rapidly removed at various times from 10 to 30 min after furosemide administration and processed for transmission electron microscopy. Control animals were found to have reversible edema of the stria vascularis. Experimental animals had variable findings. Those animals pretreated with penicillin had virtually no edema of the stria vascularis at any time. Salicylate and probenecid pretreated animals had significantly less edema from one to 10 min after furosemide injection, but more edema than controls at later times. These findings suggest a discrepancy between ultrastructural pathology and functional status of the cochlea in experimental animals pretreated with probenecid or sodium salicylate followed by furosemide. On the other hand, good structure function correlations were seen in controls and in experimental animals pretreated with penicillin.
Rybak
L P
LP
Department of Surgery, School of Medicine, Southern Illinois University, Springfield 62794-9230.
Whitworth
C
C
Weberg
A
A
Scott
V
V
D000143:Acids / Q000494:pharmacology*; D000818:Animals; D015834:Cochlear Diseases / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D005072:Evoked Potentials, Auditory / Q000187:drug effects; D005665:Furosemide / Q000633:toxicity*; D008854:Microscopy, Electron; D010400:Penicillin G / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D012980:Sodium Salicylate / Q000494:pharmacology; D013316:Stria Vascularis / Q000187:drug effects* / Q000473:pathology / Q000502:physiology
D000143:Acids; D005665:Furosemide; D011339:Probenecid; D010400:Penicillin G; D012980:Sodium Salicylate