title: The nasal response to histamine challenge: effect of the pollen season and immunotherapy.
abstract: To evaluate changes in the nasal response to histamine, we challenged 19 subjects with allergic rhinitis caused by ragweed (RW) before, during, and after the RW season with increasing doses of histamine diphosphate. We compared their response, as measured by symptoms and the levels of TAME-esterase activity and albumin recovered in the nasal lavage fluid, with response of two groups with allergic rhinitis undergoing immunotherapy with moderate-dose (N = 16) and high-dose (N = 11) RW (2 and 24 micrograms of antigen E [Amb a I] as maintenance dose, respectively). Four challenges with histamine were performed in each group: before, at the peak of, near the end of, and 2 weeks after the RW season. The three groups of subjects had similar skin sensitivity to antigen and levels of TAME-esterase activity and albumin recovered from nasal lavages after histamine challenge performed before seasonal exposure. Symptom diaries obtained throughout the season revealed a significant reduction only in the high-dose immunotherapy-treated group. At the peak of the season, the untreated group had more symptoms in response to the challenge compared with the challenges before and after the season (p = 0.04 for both groups). The saline challenge occurring before challenging with histamine also demonstrated a significant increase at the peak of the season compared with increases before the season (p = 0.02). This observation was also true for the levels of albumin and TAME-esterase activity. If the response after saline challenge was subtracted from each response after histamine challenge, no difference was found in the results between any of the visits.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine (Division of Clinical Immunology), Johns Hopkins University School of Medicine, Baltimore, Md.
authors.forename: A M
authors.initials: AM
authors.lastname: Majchel
authors.forename: D
authors.initials: D
authors.lastname: Proud
authors.forename: L
authors.initials: L
authors.lastname: Freidhoff
authors.forename: P S
authors.initials: PS
authors.lastname: Creticos
authors.forename: P S
authors.initials: PS
authors.lastname: Norman
authors.forename: R M
authors.initials: RM
authors.lastname: Naclerio
mesh_terms: D000328:Adult; D000418:Albumins / Q000032:analysis; D000485:Allergens / Q000008:administration & dosage / Q000276:immunology*; D003888:Desensitization, Immunologic / Q000379:methods; D004306:Dose-Response Relationship, Immunologic; D006632:Histamine / Q000031:analogs & derivatives*; D006801:Humans; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism; D009299:Nasal Provocation Tests / Q000379:methods; D010447:Peptide Hydrolases / Q000032:analysis; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis / Q000378:metabolism / Q000628:therapy; D012621:Seasons
chemical_list: D000418:Albumins; D000485:Allergens; D006632:Histamine; D010447:Peptide Hydrolases; C023073:tosylarginine methyl ester hydrolase; C027556:histamine phosphate