title: Promoter specificity of the two transcriptional activation functions of the human oestrogen receptor in yeast.
abstract: We have previously demonstrated that the human oestrogen receptor (hER) contains two transcriptional activation functions located in the N-terminal region (TAF-1) and in the hormone binding domain (TAF-2), which can act both independently and synergistically in a promoter- and cell-specific manner in animal cells. We have also demonstrated that hER can activate transcription from chimaeric oestrogen-responsive GAL1 promoters in yeast, and shown that transcriptional activation was due to TAF-1, whereas TAF-2 showed little, if any, transcriptional activity on these promoters. By using a more complex promoter derived from the URA3 gene, we now show that TAF-2 is also active in yeast, and that the activities of TAF-1 and TAF-2 are promoter-context-specific in yeast. We also confirm that the agonistic activity of 4-hydroxytamoxifen (OHT) can be ascribed to the activity of TAF-1.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Strasburg, France.
authors.forename: D
authors.initials: D
authors.lastname: Metzger
authors.forename: R
authors.initials: R
authors.lastname: Losson
authors.forename: J M
authors.initials: JM
authors.lastname: Bornert
authors.forename: Y
authors.initials: Y
authors.lastname: Lemoine
authors.forename: P
authors.initials: P
authors.lastname: Chambon
mesh_terms: D001483:Base Sequence; D004967:Estrogens / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D011960:Receptors, Estrogen / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013629:Tamoxifen / Q000031:analogs & derivatives / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D004967:Estrogens; D009838:Oligodeoxyribonucleotides; D011960:Receptors, Estrogen; D014157:Transcription Factors; D013629:Tamoxifen; C016601:afimoxifene