Effect of interleukin-1 beta on gene expressions and functions of fibroblastic cells derived from human periodontal ligament.
The present study shows the effect of interleukin-1 beta (IL-1 beta) on some gene expressions and functions of fibroblastic cells (HPLF) derived from human periodontal ligament. HPLF were used at passages number 5 to 10. IL-1 beta increased DNA synthesis in both a dose- and an incubation time-dependent manner. IL-1 beta in combination with tumor-necrosis factor alpha or transforming growth factor beta synergistically stimulated the DNA synthesis in the cells. Since many studies have shown that the c-myc oncogene is involved in cell proliferation and differentiation, the effect of IL-1 beta on c-myc messenger RNA (mRNA) level in HPLF was examined. IL-1 beta induced a marked c-myc mRNA level in the cells at 90 minutes after initiation of the cytokine treatment. On the other hand, IL-1 beta significantly inhibited alkaline phosphatase (ALP) activity of the cells in a dose-dependent manner. Also an inhibitory effect was observed on the liver/bone/kidney ALP mRNA level of the cells, and this inhibition by IL-1 beta was dose- and incubation time-dependent. These results suggest that IL-1 beta is a regulatory cytokine involved in the regeneration of the human periodontal ligament.
Takeshita
A
A
Department of Oral Microbiology, Meikai University School of Dentistry, Saitama, Japan.
Niu
Z G
ZG
Hanazawa
S
S
Takara
I
I
Higuchi
H
H
Katayama
I
I
Kitano
S
S
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors; D015152:Blotting, Northern; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D016259:Genes, myc / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology / Q000502:physiology*; D010513:Periodontal Ligament / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*
D007375:Interleukin-1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D004247:DNA; D000469:Alkaline Phosphatase