Antibodies to brevetoxin B: serologic differentiation of brevetoxin B and brevetoxin A.
A brevetoxin B immunogen was prepared by reacting brevetoxin B with the amino groups of bovine albumin followed by treatment with sodium borohydride. Immunized rabbits produced antibodies that bound [3H]brevetoxin B-alcohol. This binding increased with repeated immunization (KD for one rabbit after three courses of immunization was about 10 pM). Brevetoxin B inhibited greater than 500 times more effectively than brevetoxin A. When tested in a competitive binding assay with [3H]brevetoxin B-alcohol-brevetoxin B antibody, the alcohol was six times more effective as an inhibitor than brevetoxin B. Despite some similarities in structure with brevetoxin A, the antibodies to brevetoxin B did not recognize the backbone of brevetoxin A.
Levine
L
L
Department of Biochemistry, Brandeis University, Waltham, MA 02254.
Shimizu
Y
Y
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001667:Binding, Competitive; D004141:Dinoflagellida; D007106:Immune Sera / Q000276:immunology; D008387:Marine Toxins / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D015394:Molecular Structure; D035964:Oxocins; D000095702:Polyether Toxins
D000906:Antibodies; D007106:Immune Sera; D008387:Marine Toxins; D035964:Oxocins; C043466:brevetoxin B; D000095702:Polyether Toxins; C053342:brevetoxin