Gene-centromere linkage mapping by PCR analysis of individual oocytes.
We describe a general method of determining the recombination fraction between a polymorphic locus and the centromere in any species where single oocytes can be obtained. After removal of the first polar body, each oocyte is analyzed by PCR. The frequency of oocytes heterozygous at the polymorphic locus is used to estimate the recombination fraction. We estimate a recombination fraction of 0.15 between the mouse major histocompatibility complex (H-2) and the centromere of chromosome 17.
Cui
X
X
Molecular Biology Section, University of Southern California, Los Angeles 90089-1340.
Gerwin
J
J
Navidi
W
W
Li
H
H
Kuehn
M
M
Arnheim
N
N
D000818:Animals; D001483:Base Sequence; D002503:Centromere; D002874:Chromosome Mapping / Q000379:methods*; D004247:DNA / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D006183:H-2 Antigens / Q000235:genetics; D006579:Heterozygote; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009865:Oocytes; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic
D006183:H-2 Antigens; D004247:DNA