title: Electrotransfection of protoplasts from tomato, wild tomato, barley and chrysanthemum with tobacco mosaic virus RNA.
abstract: Protoplasts isolated from tomato, wild tomato, barley and chrysanthemum were electrotransfected with tobacco mosaic virus (TMV) RNA under almost the same optimum electric conditions: five square DC pulses of 50 microseconds duration at 500 to 800 V/cm, with the protoplasts suspended at 2 x 10(5)/ml in 0.5 M-mannitol containing 100 microM-MgCl2 and 10 to 20 micrograms/ml TMV RNA. ELISAs of these transfected protoplasts showed that the yields and the growth curves of the virus were quite similar, indicating a lack of host specificity in the initially infected cells of these plants.
authors.affiliation: Laboratory of Plant Pathology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Matsunaga
authors.forename: K
authors.initials: K
authors.lastname: Sawamura
authors.forename: M
authors.initials: M
authors.lastname: De Kok
authors.forename: T
authors.initials: T
authors.lastname: Makino
authors.forename: K
authors.initials: K
authors.lastname: Miki
authors.forename: M
authors.initials: M
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Tsuchizaki
authors.forename: T
authors.initials: T
authors.lastname: Hibi
mesh_terms: D011723:Chrysanthemum cinerariifolium / Q000235:genetics; D001467:Hordeum / Q000235:genetics; D010944:Plants / Q000235:genetics*; D011523:Protoplasts; D012367:RNA, Viral / Q000235:genetics*; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014162:Transfection / Q000235:genetics*
chemical_list: D012367:RNA, Viral