Creatine kinase isoforms: investigation of inhibitors of in vitro degradation and establishment of a reference range.
The in vitro stability of creatine kinase isoforms was examined by separation with high voltage electrophoresis. The effect of inhibitors of carboxypeptidase was evaluated. Preservation of samples is essential to inhibit in vitro changes in isoform pattern. EDTA at a final concentration of 15 mmol/L is recommended. Using appropriately preserved samples, normal reference intervals for the MM isoforms have been established.
Davies
J
J
Department of Chemical Pathology, Royal Gwent Hospital, Newport, UK.
Reynolds
T
T
Penney
M D
MD
D002268:Carboxypeptidases / Q000494:pharmacology; D003402:Creatine Kinase / Q000378:metabolism*; D004586:Electrophoresis; D004795:Enzyme Stability / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes; D012016:Reference Values
D007527:Isoenzymes; D003402:Creatine Kinase; D002268:Carboxypeptidases