title: Analysis of synthetic peptides using matrix-assisted laser desorption ionization mass spectrometry.
abstract: Matrix-assisted laser desorption ionization mass spectroscopy (LDI MS), a novel method for analysis of large molecules, has been used for characterization of synthetic peptides and their by-products. The potential of LDI MS is demonstrated by analyzing crude synthetic peptides representing typical members of newly designed peptides and proteins. In the first case, a fragment condensation reaction yielding a highly hydrophobic six-helic bundle template-assembled synthetic protein (TASP) is monitored. Then, a crude 19-mer peptide designed to adopt an amphiphilic alpha-helical structure and its by-products from SPPS are identified. Finally, analysis of crude hirulog-1, a 20-mer peptide designed as a thrombin inhibitor, using C18 reversed phase high performance liquid chromatography (RP HPLC), capillary electrophoresis (CE) and LDI MS, manifests the potential of the latter method.
authors.affiliation: Ciba-Geigy Ltd., Basel, Switzerland.
authors.forename: V
authors.initials: V
authors.lastname: Steiner
authors.forename: K O
authors.initials: KO
authors.lastname: Börnsen
authors.forename: M
authors.initials: M
authors.lastname: Schär
authors.forename: E
authors.initials: E
authors.lastname: Gassmann
authors.forename: S
authors.initials: S
authors.lastname: Hoffstetter-Kuhn
authors.forename: H
authors.initials: H
authors.lastname: Rink
authors.forename: M
authors.initials: M
authors.lastname: Mutter
mesh_terms: D000595:Amino Acid Sequence; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D013917:Thrombin / Q000037:antagonists & inhibitors
chemical_list: D010455:Peptides; D013917:Thrombin