title: Variants of the Xenopus laevis ribosomal transcription factor xUBF are developmentally regulated by differential splicing.
abstract: XUBF is a Xenopus ribosomal transcription factor of the HMG-box family which contains five tandemly disposed homologies to the HMG1 & 2 DNA binding domains. XUBF has been isolated as a protein doublet and two cDNAs encoding the two molecular weight variants have been characterised. The major two forms of xUBF identified differ by the presence or absence of a 22 amino acid segment lying between HMG-boxes 3 and 4. Here we show that the mRNAs for these two forms of xUBF are regulated during development and differentiation over a range of nearly 20 fold. By isolating two of the xUBF genes, it was possible to show that both encoded the variable 22 amino acid segment in exon 12. Oocyte splicing assays and the sequencing of PCR-generated cDNA fragments, demonstrated that the transcripts from one of these genes were differentially spliced in a developmentally regulated manner. Transcripts from the second gene were found to be predominantly or exclusively spliced to produce the lower molecular weight form of xUBF. Expression of a high molecular weight form from yet a third gene was also detected. Although the intron-exon structures of the Xenopus and mouse UBF genes were found to be essentially identical, the differential splicing of exon 8 found in mammals, was not detected in Xenopus.
authors.affiliation: Centre de Recherche en Cancérologie, Université Laval, Hôtel-Dieu de Québec, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Guimond
authors.forename: T
authors.initials: T
authors.lastname: Moss
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012326:RNA Splicing / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000235:genetics* / Q000254:growth & development
chemical_list: D014157:Transcription Factors; C062332:UBFT protein, Xenopus; D029867:Xenopus Proteins