title: Quail neuroretina c-Rmil(B-raf) proto-oncogene cDNAs encode two proteins of 93.5 and 95 kDa resulting from alternative splicing.
abstract: c-Rmil is the cellular allele of the v-Rmil oncogene transduced during in vitro passaging of Rous-associated virus type 1 in chicken embryonic neuroretina (NR) cells. The c-Rmil proto-oncogene is the avian homolog of the mammalian B-raf gene and belongs to the mil/raf oncogene family of serine/threonine protein kinases. The c-Rmil/B-raf gene is preferentially expressed in avian and mammalian neural tissues. Two c-Rmil cDNA species, resulting from an alternative splicing mechanism, were isolated from quail embryonic NR cDNA libraries. They encode two proteins of 767 and 807 amino acids that differ by the presence of an alternative exon, located upstream of the kinase domain. Expression of these cDNAs in COS-1 cells leads to the synthesis of two proteins with apparent molecular weights of 93.5 and 95 kDa, recognized by an Rmil-specific antiserum. Both proteins are phosphorylated in an immune complex kinase assay. A protein of 94 kDa is also immunoprecipitated in avian NR cells and is identical to the 93.5-kDa protein expressed in COS-1 cells, as shown by Staphylococcus aureus V8 protease mapping. The c-Rmil proteins contain the three conserved regions previously identified in mil/raf protein kinases. In addition, they contain amino-terminal sequences that are not present in the other mil/raf proteins identified to date. These additional sequences may define a novel functional domain for c-Rmil/B-raf and could play a role in signal transduction in neural cells.
authors.affiliation: Unité de Recherche Associée 1443 du CNRS, Institut Curie, Centre Universitaire, Orsay, France.
authors.forename: A
authors.initials: A
authors.lastname: Eychène
authors.forename: J V
authors.initials: JV
authors.lastname: Barnier
authors.forename: P
authors.initials: P
authors.lastname: Dézélée
authors.forename: M
authors.initials: M
authors.lastname: Marx
authors.forename: D
authors.initials: D
authors.lastname: Laugier
authors.forename: I
authors.initials: I
authors.lastname: Calogeraki
authors.forename: G
authors.initials: G
authors.lastname: Calothy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D015723:Gene Library; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf; D011784:Quail / Q000196:embryology; D012326:RNA Splicing; D012160:Retina / Q000196:embryology
chemical_list: D011518:Proto-Oncogene Proteins; D011494:Protein Kinases; D019908:Proto-Oncogene Proteins c-raf