A novel agarose acrobeads protein A column for selective immunoadsorbance of whole blood: performance, specificity and safety.
The present study describes the performance and safety parameters of a novel column composed of protein A linked to acrobeads. Hemoperfusion of anticoagulated whole human blood was carried out in a closed system. Specific adsorption of IgG was documented in whole blood, plasma and IgG diluted in saline. Specific activity of the column was 3.52 mg/ml beads. These studies revealed slight changes in platelet counts and minor hemolysis. The relatively short period needed for maximal hemoperfusion effect and safety may enable the use of this novel therapeutic approach in clinical practice.
Nathan
I
I
Ben-Gurion University, Faculty of Health Sciences, Beer Sheva, Israel.
Aharon
M
M
Reisenfeld
G
G
Dvilansky
A
A
D001672:Biocompatible Materials; D001769:Blood; D002847:Chromatography, Agarose; D006464:Hemoperfusion / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D007163:Immunosorbent Techniques; D008863:Microspheres; D010949:Plasma / Q000737:chemistry; D012680:Sensitivity and Specificity; D013205:Staphylococcal Protein A / Q000378:metabolism
D001672:Biocompatible Materials; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A