Determination of catecholamines in urine by liquid chromatography and electrochemical detection after on-line sample purification on immobilized boronic acid.
Norepinephrine, epinephrine and dopamine in urine were measured by an automated liquid chromatographic method. After sample purification on a column containing silica-immobilized boronic acid, which showed great affinity for catecholamines at neutral pH, the catecholamines were eluted by backflushing with an acidic mobile phase and transferred to a cation exchanger for separation. Detection was performed electrochemically and the relative standard deviation was 2% for the analysis of endogenous concentrations in human urine.
Eriksson
B M
BM
Bioanalytical Chemistry, Astra Hässle AB, Mölndal, Sweden.
Wikström
M
M
D001897:Boronic Acids / Q000737:chemistry; D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D004563:Electrochemistry; D006801:Humans
D001897:Boronic Acids; D002395:Catecholamines