New mRNA probes for hippocampal responses to entorhinal cortex lesions in the adult male rat: a preliminary report.
Three new mRNA responses were found in the hippocampus of the adult male rat after entorhinal cortex lesions (ECL) that induce synaptic reorganization. Hippocampus cDNA libraries were screened by a subtractive hybridization strategy designed to detect ECL-induced mRNAs. Partial sequencing showed clones with similarities to mouse vimentin, ferritin, and polypeptide 7B-2. A sequence similar to mouse SNAP-25 sequence was also detected. Using a mouse SNAP-25 probe, rat SNAP-25 mRNA increased in the hippocampus after ECL.
Day
J R
JR
Neurogerontology Division, Andrus Gerontology Center, Los Angeles, California.
Min
B H
BH
Laping
N J
NJ
Martin
G
G
Osterburg
H H
HH
Finch
C E
CE
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005293:Ferritins / Q000235:genetics; D007839:Functional Laterality; D015723:Gene Library; D006624:Hippocampus / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010957:Plasmids; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012689:Sequence Homology, Nucleic Acid; D050825:Synaptosomal-Associated Protein 25; D014746:Vimentin / Q000235:genetics
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D015347:RNA Probes; D012333:RNA, Messenger; C494209:Snap25 protein, mouse; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25; C075455:TB-2 polypeptide; D014746:Vimentin; D005293:Ferritins