title: Type II corticosteroid receptor-like immunoreactivity in the rat cerebellar cortex: differential regulation by corticosterone.
abstract: Type II corticosteroid receptor-like immunoreactivity (type II-ir) was localized at the light- and electron-microscopic levels in the rat cerebellar cortex using BUGR2 monoclonal antibody. In intact rats, type II-ir was observed in the nuclei of basket, stellate, Golgi and Purkinje cells. After 1 week of adrenalectomy, type II-ir was barely resolvable in basket, stellate, Golgi and most Purkinje cells. Vermal Purkinje cells showed intense nuclear and cytoplasmic type II-ir. After 4 weeks of adrenalectomy, type II-ir was markedly reduced in most vermal Purkinje cells, however a few in lobules 1-3 and 9 and 10 retained diffuse immunoreactivity. Acute treatment with corticosterone restored nuclear type II-ir to basket, stellate, Golgi and Purkinje cells in the cerebellar hemispheres, flocculi and paraflocculi. In the vermis most Purkinje cells showed nuclear type II-ir following corticosterone treatment. Diffuse type II-ir was, however, retained in a few Purkinje cells in vermal lobules 1-3, 9 and 10. The distribution of GABA-like immunoreactivity (GABA-ir) in the cerebellar cortex was not altered by adrenalectomy or acute corticosterone treatment. 30-60% of type II-ir Purkinje cells in intact rats were GABA-ir. In adrenalectomized and corticosterone-treated rats, a similar proportion of type II-ir Purkinje cells were GABA-ir. The differential regulation of neuronal type II-ir in the cerebellum by corticosterone may account for some of the known effects of glucocorticoids on motor coordination.
authors.affiliation: Department of Anatomy, Tulane University School of Medicine, New Orleans, La. 70112.
authors.forename: R S
authors.initials: RS
authors.lastname: Ahima
authors.forename: C N
authors.initials: CN
authors.lastname: Tagoe
authors.forename: R E
authors.initials: RE
authors.lastname: Harlan
mesh_terms: D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000818:Animals; D000911:Antibodies, Monoclonal; D002525:Cerebellar Cortex / Q000737:chemistry*; D003345:Corticosterone / Q000502:physiology*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000032:analysis*; D013347:Subcellular Fractions / Q000737:chemistry*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D011965:Receptors, Glucocorticoid; D005680:gamma-Aminobutyric Acid; D003345:Corticosterone