Cloning and nucleotide sequence of the Vibrio proteolyticus aminopeptidase gene.
The gene encoding the Vibrio proteolyticus aminopeptidase was cloned and sequenced and its amino acid sequence was deduced. The gene encodes a 54 kDa protein, larger than the previously reported size of 30 kDa for the purified aminopeptidase. Sequence alignments revealed a 43-45% homology with two other Vibrio sp. extracellular proteinases.
Van Heeke
G
G
Protein Expression Laboratory, University of Florida, Gainesville 32610.
Denslow
S
S
Watkins
J R
JR
Wilson
K J
KJ
Wagner
F W
FW
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014733:Vibrio / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D000626:Aminopeptidases; C038222:bacterial leucyl aminopeptidase