Transfer of conjugal elements in oral black-pigmented Bacteroides (Prevotella) spp. involves DNA rearrangements.
Conjugal genetic elements in isolates of oral black-pigmented Bacteroides denticola (Prevotella denticola) and B. intermedius (P. intermedia) transfer tetracycline and penicillin resistance in the absence of plasmids. Transverse alternating-field electrophoresis of restricted chromosomal DNAs from transconjugants revealed arrangements indicating that transfer and insertion can involve more than one 60-kb copy of the elements and occurs at strongly preferred sites in the recipient chromosome.
Guiney
D G
DG
Department of Medicine, University of California San Diego 92103.
Hasegawa
P
P
D001439:Bacteroides / Q000187:drug effects / Q000235:genetics*; D015139:Blotting, Southern; D003227:Conjugation, Genetic / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D010403:Penicillin Resistance / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics
D004269:DNA, Bacterial