YAC mapping by FISH using Alu-PCR-generated probes.
Human genomic mapping has been greatly advanced by the independent development of three new methods: large DNA fragment cloning in yeast artificial chromosomes, amplification from complex DNAs of human specific segments by Alu-PCR, and high-resolution localization of complex DNA probes by fluorescent in situ hybridization. We describe here the combination of these three analytical tools for efficient and accurate localization of randomly screened or especially selected human YAC recombinants to chromosome 11. We map a YAC clone encompassing the pepsinogen A (PGA) locus to 11q13.1-11q13.3.
Breen
M
M
MRC Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom.
Arveiler
B
B
Murray
I
I
Gosden
J R
JR
Porteous
D J
DJ
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal; D002880:Chromosomes, Human, Pair 11; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005453:Fluorescence; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D004247:DNA