title: Trehalase activity and its regulation during growth of Saccharomyces cerevisiae.
abstract: Trehalase activity decreased in 95% at the onset of the transition phase of growth of S. cerevisiae. The question which we raised was whether this phenomenon was due to proteolysis or to conversion of the enzyme to a less active form (dephosphorylation). Immunological methods allowed to identify the presence of the trehalase protein during cell growth. At the same stage of growth, an increase in the non-phosphorylated enzyme was detected "in vitro". Results utilizing mutant strains also indicated that regulation occurred by interconversion of forms. The same mechanism also seems to control trehalase activity in non proliferating conditions.
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brasil.
authors.forename: C C
authors.initials: CC
authors.lastname: Coutinho
authors.forename: J T
authors.initials: JT
authors.lastname: Silva
authors.forename: A D
authors.initials: AD
authors.lastname: Panek
mesh_terms: D015153:Blotting, Western; D004789:Enzyme Activation; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development; D014198:Trehalase / Q000378:metabolism*
chemical_list: D014198:Trehalase; D005947:Glucose