Effects of 1-(halogenalkoxy)alkyl-5-fluorouracil derivatives on cell growth, cell volume and nucleus size of mouse lymphoma cells.
The effects of three 1-(halogenalkoxy)alkyl-5-fluorouracil derivatives on cultured mouse lymphoma cells were studied and compared with those of N-methyl-bis-(2-chloroethyl)amine hydrochloride (Lost). The derivatives exert only little influence on cell proliferation and cell volume. However, all derivatives cause a concentration-dependent nucleus contraction, probably due to DNA cross-linkings. Bromodesoxyuridine modulates the effects of the derivatives on the DNA, leading to swelling of the nucleus, which may be caused by DNA strandbreaks. It is suggested that the derivatives exert synergistic effects with other factors. It is concluded that these studies are suitable for the prescreening of agents to be investigated in mutagenicity tests.
Reitz
M
M
Institut für Physiologische Chemie, Universität Mainz, BRD.
Jaeger
K H
KH
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology; D005472:Fluorouracil / Q000031:analogs & derivatives* / Q000494:pharmacology; D008223:Lymphoma / Q000473:pathology*; D051379:Mice; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
C075351:1-((2-chloroethoxy)methyl)-5-fluorouracil; C075350:1-((6-chlorohexyloxy)methyl)-5-fluorouracil; C075349:1-((2-(2-chloroethoxy)ethoxy)methyl)-5-fluorouracil; D005472:Fluorouracil