Effect of ingestion of excess methionine diet on aging of erythrocytes in mice.
Accelerated senescence prone mice and resistant mice (SAM-P/1 and SAM-R/1) were fed 10% casein diet or that supplemented with 2.5% methionine (Met) diet for 4 weeks. Erythrocytes withdrawn from animals of each group were separated by density gradient centrifugation into four fractions of different cell density, that is, different age of erythrocytes, and were analyzed for the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and the level of oxidized protein. Excess Met decreased the specific activity of GSH-Px in each fraction and the whole population of erythrocytes in SAM-P/1 but not in SAM-R/1. The changes in SOD activities were least significant among the erythrocyte fractions of every dietary group. The oxidized protein level in cell lysate increased with the increase of cell density in both strains of animals. These results were interpreted as indicating that excess Met had a positive effect on the erythrocytes senescence, and suggested that the activity of GSH-Px may be a useful enzymatic marker for the age of erythrocytes.
He
P
P
Research Institute for Food Science, Kyoto University, Japan.
Yasumoto
K
K
D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D005591:Chemical Fractionation; D004905:Erythrocyte Aging / Q000187:drug effects*; D004912:Erythrocytes / Q000201:enzymology; D006454:Hemoglobins / Q000032:analysis; D008715:Methionine / Q000008:administration & dosage / Q000009:adverse effects*; D051379:Mice
D001798:Blood Proteins; D006454:Hemoglobins; D008715:Methionine