Assessment of iodohexadecenoic acid as a tracer of fatty acid metabolism by external detection: a study on isolated rat heart.
Labelled fatty acids have been proposed to explore cardiac metabolism. For the analysis of the external detection curve obtained with 16-iodo 9-hexadecenoic acid (IHA), we developed a mathematical 4-compartment model with compartments 0, 1, 2 and 3 representing vascular IHA, intracellular IHA, esterified forms and iodide, respectively. This model, used here for isolated rat hearts perfused in a recirculating system, is validated by an intracellular analysis, then tested in various metabolic conditions. Thus, the mathematical analysis of the external detection curve gives us numerical data on IHA metabolism, especially the distribution between degradation and storage. Our results confirm the suitability of IHA for assessing myocardial metabolism.
Keriel
C M
CM
Laboratoire de Physiologie Cellulaire Cardiaque, Université Joseph Fourier, Grenoble, France.
Dubois
F M
FM
Marti Batlle
D S
DS
Pernin
C R
CR
Leverve
X M
XM
Mathieu
J P
JP
Comet
M
M
Cuchet
P J
PJ
D000818:Animals; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D006339:Heart Rate / Q000502:physiology; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008432:Mathematical Computing; D008954:Models, Biological; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism*; D010169:Palmitic Acids / Q000378:metabolism / Q000493:pharmacokinetics; D051381:Rats; D011919:Rats, Inbred Strains
D005227:Fatty Acids; D007457:Iodine Radioisotopes; D010169:Palmitic Acids; C040433:16-iodo-9-hexadecenoic acid