title: A rat cerebellar protein containing the cdc10/SWI6 motif.
abstract: Systematic analysis of soluble proteins in developing rat cerebellum by an automated two-dimensional liquid-chromatography system detected a number of proteins which increased transiently during the initial stage of postnatal development. One of the proteins, V-1, was isolated using a liquid-chromatography system, and its amino acid sequence was determined by analysis of the purified protein. The sequence showed that the V-1 protein consists of 117 amino acids with an acetylated N-terminus, and has 2.5 internal sequence repeats of 33 amino acids. Computer retrieval of the sequence indicated that the repeated sequences have a structural characteristics of the cdc10/SWI6 motif, which is found in a series of proteins, including those involved in cell-cycle control and cell-fate determination in yeast, Drosophila melanogaster and Caenorhabditis elegans. The structure of V-1, coupled with its controlled expression in early postnatal development, implies a potential role for V-1 in cerebellar morphogenesis.
authors.affiliation: Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Taoka
authors.forename: T
authors.initials: T
authors.lastname: Yamakuni
authors.forename: S Y
authors.initials: SY
authors.lastname: Song
authors.forename: Y
authors.initials: Y
authors.lastname: Yamakawa
authors.forename: K
authors.initials: K
authors.lastname: Seta
authors.forename: T
authors.initials: T
authors.lastname: Okuyama
authors.forename: T
authors.initials: T
authors.lastname: Isobe
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D018797:Cell Cycle Proteins; D002531:Cerebellum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins / Q000737:chemistry*; D020558:GTP Phosphohydrolases; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D051381:Rats; D011919:Rats, Inbred Strains; D029702:Schizosaccharomyces pombe Proteins; D058112:Septins; D016415:Sequence Alignment; D014157:Transcription Factors
chemical_list: D000596:Amino Acids; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins; D009419:Nerve Tissue Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C061178:cdc10 protein, S pombe; D020558:GTP Phosphohydrolases; C512894:Septin7 protein, rat; D058112:Septins