Commitment of abdominal neuroblasts in Drosophila to a male or female fate is dependent on genes of the sex-determining hierarchy.
Adult specific neurons in the central nervous system of holometabolous insects are generated by the postembryonic divisions of neuronal stem cells (neuroblasts). In the ventral nervous system of Drosophila melanogaster, sex-specific divisions by a set of abdominal neuroblasts occur during larval and early pupal stages. Animals mutant for several sex-determining genes were analyzed to determine the genetic regulation of neuroblast commitment to the male or female pattern of division and the time during development when these decisions are made. We have found that the choice of the sexual pathway taken by sex-specific neuroblasts depends on the expression of one of these genes, doublesex (dsx). In the absence of any functional dxs+ products, the sex-specific neuroblasts fail to undergo any postembryonic divisions in male or female larval nervous systems. From the analysis of intersexes generated by dominant alleles of dsx, it has been concluded that the same neuroblasts provide the sex-specific neuroblasts in both male and female central nervous systems. The time when neuroblasts become committed to generate their sex-specific divisions were identified by shifting tra-2ts flies between the male- and female-specifying temperatures at various times during larval development. Neuroblasts become determined to adopt a male or female state at the end of the first larval instar, a time when abdominal neuroblasts enter their first postembryonic S-phase.
Taylor
B J
BJ
Department of Zoology, University of Washington, Seattle 98195.
Truman
J W
JW
D000005:Abdomen / Q000033:anatomy & histology; D000818:Animals; D002490:Central Nervous System / Q000196:embryology*; D004330:Drosophila / Q000033:anatomy & histology / Q000235:genetics*; D005260:Female; D005796:Genes / Q000235:genetics*; D008297:Male; D009024:Morphogenesis / Q000235:genetics; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000166:cytology*; D010641:Phenotype; D012727:Sex Characteristics; D012733:Sex Differentiation / Q000235:genetics*; D013234:Stem Cells / Q000166:cytology*; D013696:Temperature