title: Study on fatty acid binding by proteins in yeast. Dissimilar results in Saccharomyces cerevisiae and Yarrowia lipolytica.
abstract: 1. The presence of soluble proteins with fatty acid binding activity was investigated in cell-free extracts from Saccharomyces cerevisiae and Yarrowia lipolytica cultures. 2. No significant fatty acid binding by proteins was detected in S. cerevisiae, even when grown on a fatty acid-rich medium, thus indicating that such proteins are not essential to fatty acid metabolism. 3. An inducible fatty acid binding protein (K0.5 = 3-4 microM) was found in Y. lipolytica which had grown on a minimal medium with palmitate as the sole source of carbon and energy. 4. The relative molecular mass of this protein was 100,000 as inferred from Sephacryl S-200 gel filtration.
authors.affiliation: Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: E C
authors.initials: EC
authors.lastname: Dell'Angelica
authors.forename: C A
authors.initials: CA
authors.lastname: Stella
authors.forename: M R
authors.initials: MR
authors.lastname: Ermácora
authors.forename: E H
authors.initials: EH
authors.lastname: Ramos
authors.forename: J A
authors.initials: JA
authors.lastname: Santome
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D002850:Chromatography, Gel; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D007700:Kinetics; D009363:Neoplasm Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005656:Fungal Proteins; D009363:Neoplasm Proteins