HER-2/neu gene in primary and local metastatic axillary lymph nodes in human breast tumors.
In order to verify whether the HER-2/neu gene is involved in the initial phases of neoplastic disease or in its progression, we evaluated the amplification and overexpression of this gene in the primary tumor and in synchronous metastatic axillary lymph nodes of 26 women with operable breast cancer. HER-2/neu was amplified in 35% and overexpressed in 33% of the primary sites; similar percentages were found in lymph nodes. The clear correlation between the two disease sites regarding gene, mRNA and protein levels, supports the hypothesis that this gene is involved in the initial and invasive phases of neoplasia. Its actual role with respect to other biological tumor characteristics during the metastatic process should be investigated further.
Tommasi
S
S
Experimental and Clinical Oncology Laboratory, Oncology Institute, Bari, Italy.
Giannella
C
C
Paradiso
A
A
Barletta
A
A
Mangia
A
A
Simone
G
G
Primavera
A T
AT
Albarani
V
V
Schittulli
F
F
Longo
S
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005784:Gene Amplification / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis / Q000235:genetics*; D008875:Middle Aged; D009857:Oncogenes / Q000235:genetics*; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis
D012333:RNA, Messenger; D012334:RNA, Neoplasm