title: Post operative surveillance of human hydatidosis: evaluation of immunodiagnostic tests.
abstract: We investigated the kinetics of antibodies detected by indirect hemagglutination (IHA), IgE Elisa and immunoelectrophoresis (IEP) in patients with hydatid disease operated on and continuously followed in the pre-operative and post-operative periods. In the pre-operative phase the IgE Elisa test was found to be adequately sensitive (68.4%) compared with IHA (79%), with a ratio of IgE Elisa/IHA positivity of 87%, while IEP was positive in 55.3% of cases (IEP/IHA ratio = 70%). During post-operative follow-up IHA became negative late in patients who were cured (7 out of 11 were still positive after 4 yrs), whereas IEP and IgE Elisa became negative within 2 yrs of operation (apart from 1 patient with a persisting positive IgE Elisa 3 yrs later). However, IgE Elisa appeared clearly more sensitive in revealing postoperative recurrences (13 out of 13 patients had positive IgE Elisa, vs. 6 out of 13 IEP).
authors.affiliation: Institute of Infectious Diseases, Policlinico Monteluce, Perugia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Baldelli
authors.forename: R
authors.initials: R
authors.lastname: Papili
authors.forename: D
authors.initials: D
authors.lastname: Francisci
authors.forename: C
authors.initials: C
authors.lastname: Tassi
authors.forename: G
authors.initials: G
authors.lastname: Stagni
authors.forename: S
authors.initials: S
authors.lastname: Pauluzzi
mesh_terms: D000328:Adult; D000368:Aged; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology*; D004443:Echinococcosis / Q000276:immunology* / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D007122:Immunoelectrophoresis; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007159:Immunologic Tests; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D012008:Recurrence; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D007073:Immunoglobulin E