Regional DNA content heterogeneity in colonic adenocarcinoma: prognostic significance in patients with liver metastases.
We retrospectively examined by flow cytometry the DNA ploidy pattern in tissue blocks from 25 primary colon adenocarcinomas and their lymph node and liver metastases. Intratumoral heterogeneity was present in 22% of primary tumors and 21% of metastatic liver deposits. Intertumoral heterogeneity, measured between the primary tumor and its lymph node and liver metastases, was 0% and 20%, respectively. Of 24 patients who underwent successful resection of their liver metastases, 8 neoplasms had uniformly diploid DNA content, while 16 tumors had aneuploid DNA pattern in either the primary tumor, the metastases, or both. Five-year survival was better in the diploid group (38% vs. 7%, P = 0.10 by log rank analysis). Three of eight patients in the diploid group remain free of disease, while all 16 patients with aneuploid cell populations have died of recurrent disease.
Graham
R A
RA
Department of Surgical Oncology, New England Medical Center, Boston, Massachusetts 02111.
Teague
K
K
Mclemore
D
D
Cleary
K
K
el-Naggar
A
A
D000230:Adenocarcinoma / Q000737:chemistry* / Q000235:genetics / Q000556:secondary; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry / Q000235:genetics / Q000556:secondary*; D008198:Lymph Nodes / Q000737:chemistry; D008207:Lymphatic Metastasis / Q000235:genetics; D011003:Ploidies; D011379:Prognosis; D012004:Rectal Neoplasms / Q000737:chemistry* / Q000235:genetics; D012189:Retrospective Studies
D004273:DNA, Neoplasm