Eukaryotic cell components that bind to chlamydial elementary bodies: the histones.
HeLa-cell-membrane fractions isolated by sonication as used previously to identify chlamydial adhesins were examined by a blotting technique for binding chlamydial elementary bodies (EB). One HeLa cell protein with apparent molecular mass of 32 kDa was found to bind native EB. A monoclonal antibody (mAb) raised against this chlamydial binding host-cell protein reacted with eucaryotic histones. Histone fractions were capable of binding EB in an ELISA assay and histone H1 was identified as the chlamydial-binding host cell protein in the Hela cell membrane fraction. Probing with specific mAbs against histone H3 and DNA confirmed that chromatin components were present in the host-cell membrane extract. These data suggest that the HeLa-cell-binding chlamydial proteins were previously identified by their reaction with chromatin and not with membrane components.
Vretou
E
E
Department of Biotechnology, Hellenic Pasteur Institute, Athens, Greece.
Eliades
P
P
Psarrou
E
E
Kouvatsou
R
R
D000911:Antibodies, Monoclonal; D002448:Cell Adhesion / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002692:Chlamydia trachomatis / Q000378:metabolism*; D002843:Chromatin / Q000737:chemistry; D006367:HeLa Cells / Q000737:chemistry* / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006801:Humans
D000911:Antibodies, Monoclonal; D002843:Chromatin; D006657:Histones