Neuropeptide Y m-RNA and peptide are transiently expressed in the developing rat spinal cord.
The distribution of neuropeptide Y (NPY) m-RNA and peptide were studied during the ontogeny of the rat spinal cord, by means of in situ hybridization and immunohistochemistry. NPY expressing neurons were visualized by both techniques, although by in situ hybridization they appeared slightly more abundant than by immunohistochemistry. In the ventral horn, a few neurons transiently expressed NPY from the embryonic day 15 to birth, but not in the adult animals. In the embryonic dorsal horn, NPY expressing neurons were numerous and seen as early as embryonic day 15 (E15). In contrast, in the postnatal and the adult dorsal horn, the number of neurons expressing the NPY phenotype was dramatically lower.
Martí
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E
Dipartimento di Biologia Animale, Università di Torino, Italy.
Biffo
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S
Fasolo
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A
D000818:Animals; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009478:Neuropeptide Y / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
D009478:Neuropeptide Y; D012333:RNA, Messenger