Nucleotide sequence and transcriptional analysis of a gene encoding gp41, a structural glycoprotein of the baculovirus Autographa californica nuclear polyhedrosis virus.
We have identified and sequenced a region of the Autographa californica nuclear polyhedrosis virus (AcMNPV) genome encoding the major polyhedron-derived virus structural glycoprotein gp41. The open reading frame is located entirely within the AcMNPV SstII-M fragment. The protein sequence does not have hydrophobic regions characteristic of integral membrane proteins and contains a putative O-linked GlcNAc glycosylation site. gp41 is expressed as a late gene, with transcripts starting within two consensus late transcription start sites (TAAG) located immediately upstream of the first methionine codon. A major transcription termination signal is bypassed, possibly generating a bicistronic message. Finally, the nucleotide and protein sequences of AcMNPV and Bombyx mori nuclear polyhedrosis virus are highly conserved.
Whitford
M
M
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
Faulkner
P
P
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D005814:Genes, Viral; D016679:Genome, Viral; D006031:Glycosylation; D007313:Insecta; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000379:methods; D011487:Protein Conformation; D015183:Restriction Mapping; D014158:Transcription, Genetic; D015678:Viral Structural Proteins / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D015678:Viral Structural Proteins