title: A new deficiency mapping technique using the SOFI detector.
abstract: We present a new technique for chromosomal deficiency mapping that takes advantage of the ability of the SOFI detector to provide fast quantitative data of very weak signals. With this new strategy, in contrast to the time-consuming traditional method, all the clones corresponding to a given genomic region may be mapped for their inclusion inside a deletion with only two hybridizations, independent of the size of the genomic region to be analyzed.
authors.affiliation: Centre de Génétique, Moléculaire CNRS, Gif sur Yvette.
authors.forename: P
authors.initials: P
authors.lastname: Thérond
authors.forename: R
authors.initials: R
authors.lastname: Mastrippolito
authors.forename: H
authors.initials: H
authors.lastname: Tricoire
mesh_terms: D000818:Animals; D001345:Autoradiography; D015139:Blotting, Southern; D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000295:instrumentation*; D004252:DNA Mutational Analysis; D015342:DNA Probes; D004331:Drosophila melanogaster / Q000235:genetics; D005336:Fiber Optic Technology; D005822:Genetic Vectors; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D013997:Time Factors
chemical_list: D015342:DNA Probes