Identification of two types of homologous DNA pairing activity in mouse cells.
We have identified two types of homologous DNA pairing activity in mouse cell extracts by a strand-transfer assay. Both activities are separated from each other by anion-exchange chromatography; neither of them needs ATP. One requires magnesium ion and is stimulated by Escherichia coli single-stranded DNA binding protein, whereas the other does not require the ion and shows a higher affinity for a left-handed Z-DNA.
Mishina
Y
Y
Kihara Institute for Biological Research, Yokohama City University, Japan.
Shimokawa
T
T
Kawasaki
K
K
Arai
N
N
Shibata
T
T
Koyama
H
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D002999:Clone Cells; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D004270:DNA, Circular / Q000235:genetics / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured
D004269:DNA, Bacterial; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D000255:Adenosine Triphosphate; D008274:Magnesium