title: mRNP4, a major mRNA-binding protein from Xenopus oocytes is identical to transcription factor FRG Y2.
abstract: Previous work has revealed a striking similarity between the sequence of mRNP4, a major mRNA-binding protein of Xenopus laevis oocytes and FRG Y2, a transcription factor specific for Y-box-containing promoters. However, the apparent molecular mass of mRNP4 exceeds by 50-60% the cDNA-deduced molecular mass of FRG Y2. To resolve this discrepancy we have measured the real molecular mass of mRNP4 by sedimentation equilibrium. The molecular mass of mRNP4 closely agrees with that of FRG Y2. We conclude that mRNP4 is identical to FRG Y2 and has a dual function in oocytes. As a DNA-binding protein, mRNP4 stimulates transcription from a specific set of promoters. As a mRNA-binding protein, mRNP4 favors accumulation of the transcripts it contributes to produce.
authors.affiliation: Centre de Génétique moléculaire, Centre National de la Recherche Scientifique associé à l'Université P. et M. Curie Paris VI, France.
authors.forename: S
authors.initials: S
authors.lastname: Deschamps
authors.forename: A
authors.initials: A
authors.lastname: Viel
authors.forename: M
authors.initials: M
authors.lastname: Garrigos
authors.forename: H
authors.initials: H
authors.lastname: Denis
authors.forename: M
authors.initials: M
authors.lastname: le Maire
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009865:Oocytes / Q000502:physiology*; D010053:Ovary / Q000502:physiology; D011485:Protein Binding; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D012967:Sodium Dodecyl Sulfate; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D029867:Xenopus Proteins; D014982:Xenopus laevis
chemical_list: D016601:RNA-Binding Proteins; D014157:Transcription Factors; D029867:Xenopus Proteins; C075593:YBX2 protein, Xenopus; D012967:Sodium Dodecyl Sulfate