Peptide display on filamentous phage capsids. A new powerful tool to study protein-ligand interaction.
Peptides can be displayed on the surface of filamentous bacteriophages by fusion to phage coat proteins. It was recently shown that vast (10(8)) collections of phages, each exposing a variant of the original peptide, can be constructed and utilized as a general source of peptide ligands. By panning these libraries on a target molecule linked to a solid support it is possible to select, out of the hundreds of millions of clones, those few phages that display a peptide that binds the target molecule. Searching these libraries is a powerful tool to be applied in many areas of fundamental and applied biology.
Cesareni
G
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Department of Biology, University of Tor Vergata, Rome, Italy.
D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism*; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D015698:Genomic Library; D008024:Ligands; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
D008024:Ligands; D011994:Recombinant Proteins