title: Insulin-like growth factor II expression in the developing human brain.
abstract: Insulin-like growth factor II (IGF-II) is a polypeptide hormone with insulin-like metabolic activity and neurotrophic activity in vitro that has been implicated in human brain development. In this study, we used northern blot analysis to examine the patterns of IGF-II mRNA expression in selected regions of 18 human brains from cases ranging in age from 20 gestational weeks to 2.5 years (median age 31 gestational weeks). The expression of IGF-II mRNA was widespread throughout the brain from midgestation through the perinatal period. Each region showed a distinct developmental pattern of expression and IGF-II mRNA levels varied considerably between regions. The highest levels of expression at all ages were in leptomeninges and choroid plexus. After two postnatal months, IGF-II mRNA virtually disappeared from parenchymal regions. Beyond the perinatal period, IGF-II expression persisted primarily in choroid plexus. Transcripts of both 6.0 and 4.8 kb were detected in most brain regions. A developmental change in the relative amounts of the two transcripts occurred in choroid plexus, leptomeninges and medulla. The expression of IGF-II mRNA in the brain parenchyma during the last half of gestation correlates with a period of major brain growth and supports the hypothesis that high levels of IGF-II stimulate the proliferation and differentiation of neural cells early in development.
authors.affiliation: Department of Neurology, Children's Hospital, Boston, Massachusetts 02115.
authors.forename: P A
authors.initials: PA
authors.lastname: McKelvie
authors.forename: K M
authors.initials: KM
authors.lastname: Rosen
authors.forename: H C
authors.initials: HC
authors.lastname: Kinney
authors.forename: L
authors.initials: L
authors.lastname: Villa-Komaroff
mesh_terms: D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II