Degradation of triglycerides by a pseudomonad isolated from milk: molecular analysis of a lipase-encoding gene and its expression in Escherichia coli.
Psychrotrophic lipolytic bacteria represent a significant problem in the storage of refrigerated dairy products. A lipase-encoding gene has been cloned and characterized from a highly lipolytic strain of Pseudomonas. The nucleotide sequence of the gene predicts a polypeptide of M(r) 49,905, which was identified when the gene was expressed in Escherichia coli.
Johnson
L A
LA
School of Science, Griffith University, Nathan, Brisbane, Queensland, Australia.
Beacham
I R
IR
MacRae
I C
IC
Free
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D001673:Biodegradation, Environmental; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006041:Goats; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*
D001426:Bacterial Proteins; D014280:Triglycerides; D008049:Lipase