Binding of monoclonal anti-interleukin 2 antibody to nucleoli in acute leukemia blast cells.
Neutralizing anti-IL-2, anti-IL-3, and anti-IL-6 monoclonal antibodies (MAbs) were used for the immunoenzymatic detection of the respective cytokines in blast cells of 38 patients with acute myeloid (AML) and lymphoid (ALL) leukemias by the APAAP-technique. In 20/24 AML-cases (83%) blast cells showed intranuclear staining with MAb anti-IL-2 (DMS-1). In 17 cases reaction was restricted to the nucleoli, in 4 cases additional cytoplasmic staining was observed. Only 2/13 (15%) of the ALL cases showed anti-IL-2 staining. In contrast to IL-2, neither IL-3, IL-6 nor IL-2R alpha-chains were detected in any of the acute leukemias tested. The anti-IL-2 staining of nucleoli in AML cells is distinct from the cytoplasmic staining which is observed in PHA-activated normal lymphocytes and in a minority of AML cells.
Schwonzen
M
M
Medizinische Klinik I, Köln, Germany.
Pfreundschuh
M
M
Pohl
C
C
Schuh
A
A
Lathan
B
B
Diehl
V
V
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D001666:Binding Sites, Antibody; D002466:Cell Nucleolus / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007376:Interleukin-2 / Q000276:immunology*; D007377:Interleukin-3 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D015479:Leukemia, Myelomonocytic, Acute / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D014411:Neoplastic Stem Cells / Q000276:immunology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D007377:Interleukin-3; D015850:Interleukin-6