Immunofluorescence studies of atrophie blanche with antibodies against fibrinogen, fibrin, plasminogen activator inhibitor, factor VIII-related antigen, and collagen type IV.
In and around ulcers complicating the chronic venous insufficiency syndrome and atrophie blanche a pericapillary cuff of fibrinoid material has been described. The aim of the present study was to find out whether pericapillary cuffs are present in atrophie blanche ulcerations, whether they consist of fibrinogen and/or fibrin in comparison to normal controls, and whether this cuff is composed of other components. Skin biopsies from ten patients adjacent to atrophie blanche ulcers, and from ten controls were taken. In all patients pericapillary cuffs consisting of fibrin were found. However, no fibrinogen was found in these cuffs. In the controls no cuffs were found. In this fibrin network factor VIII-related antigen and collagen type IV were also present. The finding of plasminogen activator inhibitor-I in the pericapillary cuff in several cases may indicate that breakdown of this fibrin cuff is impaired. The possible diffusion barrier caused by the pericapillary cuff together with the pattern of vascularization may be an important event in ulcer formation and impaired ulcer healing.
Brakman
M
M
Department of Dermatology, Hospital De Lichtenberg, Amersfoort, The Netherlands.
Faber
W R
WR
Kerckhaert
J A
JA
Kraaijenhagen
R J
RJ
Hart
H C
HC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D001706:Biopsy; D003094:Collagen / Q000032:analysis*; D005260:Female; D005337:Fibrin / Q000032:analysis*; D005340:Fibrinogen / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D015849:Plasminogen Inactivators / Q000032:analysis*; D012867:Skin / Q000473:pathology*; D014647:Varicose Ulcer / Q000473:pathology*; D014689:Venous Insufficiency / Q000473:pathology*; D014841:von Willebrand Factor / Q000032:analysis*
D000911:Antibodies, Monoclonal; D015849:Plasminogen Inactivators; D014841:von Willebrand Factor; D005337:Fibrin; D005340:Fibrinogen; D003094:Collagen