32P-postlabeling analysis of adducts formed from 1-phenylazo-2-hydroxynaphthalene (Sudan I, Solvent Yellow 14) with DNA and homopolydeoxyribonucleotides.
A 32P-postlabeling assay was employed for detection and quantitation of DNA adducts formed with carcinogenic 1-phenylazo-2-hydroxynaphthalene (Sudan I, Solvent Yellow 14) activated by a peroxidase system. Enrichment of adducts by digestion with nuclease P1 or by extraction into n-butanol prior to 32P-labeling was used. Both enrichment procedures exhibited comparable results for recovery of individual DNA adduct spots. Co-chromatographic analyses of adduct spots obtained by reaction with DNA and homopolydeoxy-ribonucleotides showed that four out of the eight major Sudan I-DNA adducts were formed by reaction of activated Sudan I with deoxyadenosine or deoxyguanosine in DNA. The accuracy of quantitation of adducts by 32P-postlabeling procedure is discussed.
Stiborová
M
M
Department of Biochemistry, Faculty of Natural Sciences, Charles University, Prague, Czechoslovakia.
Frei
E
E
Schmeiser
H H
HH
Wiessler
M
M
D001345:Autoradiography; D002273:Carcinogens / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D004247:DNA / Q000737:chemistry*; D009284:Naphthols / Q000737:chemistry*; D010761:Phosphorus Radioisotopes; D011089:Polydeoxyribonucleotides / Q000737:chemistry*; D011865:Radioisotope Dilution Technique; D015719:Single-Strand Specific DNA and RNA Endonucleases
D002273:Carcinogens; D009284:Naphthols; D010761:Phosphorus Radioisotopes; D011089:Polydeoxyribonucleotides; C024336:1-phenylazo-2-naphthol; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases