Surface expressed heat-shock proteins by stressed or human immunodeficiency virus (HIV)-infected lymphoid cells represent the target for antibody-dependent cellular cytotoxicity.
The expression of heat-shock proteins (HSP) of the 72,000 MW family by Daudi and H9 lymphoma cells has been investigated by flow cytometry. It has been found that both heat-stressed and chronically human immunodeficiency virus (HIV)-infected lymphomas show an increased expression of heat-shock proteins (HSP). Moreover, murine monoclonal antibody (mAb) against 72,000 MW HSP was able to mediate antibody-dependent cellular cytotoxicity (ADCC) using peripheral blood lymphocytes (PBL) as effector cells. All target cells used in these experiments were efficiently lysed in the presence of anti-HSP antibody suggesting a role of membrane HSP in the elimination of stressed or infected cells.
Di Cesare
S
S
Department of Biology, University of Rome Tor Vergata, Italy.
Poccia
F
F
Mastino
A
A
Colizzi
V
V
D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D006360:Heat-Shock Proteins / Q000032:analysis / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D009363:Neoplasm Proteins / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000737:chemistry
D006360:Heat-Shock Proteins; D009363:Neoplasm Proteins