Eosinophil chemotactic peptide sequences in rat alpha-CGRP. Activation of a novel trophic action by neutral endopeptidase 24.11.
Rat alpha- and alpha-CGRP are substrates for endopeptidase 24.11 in vitro. Cleavage of both peptides occurs at several points, including an unusual substrate recognition site to the amino side of ala36. In alpha-CGRP this resulted in the early formation of val32-gly-ser-glu35, a sequence previously reported to be a component of the eosinophil chemotactic factor of anaphylaxis (ECF-A). The biological activity of this peptide fragment was confirmed by bioassay. Chemotactic activity in other hydrolysis fragments of both alpha- and beta-CGRP was observed. Both alpha- and beta-CGRP could thus serve as precursors to different eosinophil chemotactic peptide fragments. A novel function of endopeptidase 24.11 may be to modify rather than to terminate the biological activity of CGRP peptides.
Davies
D
D
Department of Zoology, University of Bristol, United Kingdom.
Medeiros
M S
MS
Keen
J
J
Turner
A J
AJ
Haynes
L W
LW
D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004804:Eosinophils / Q000187:drug effects / Q000502:physiology*; D008222:Lymphokines / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D015260:Neprilysin / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity
D008222:Lymphokines; D010446:Peptide Fragments; C022227:eosinophil stimulating promoter; D015260:Neprilysin; D015740:Calcitonin Gene-Related Peptide