title: Streptomycin blocks the afferent synapse of the isolated semicircular canals of the frog.
abstract: This study aimed to define the acute electrophysiological effects of the perilymphatic perfusion of streptomycin in the sensory apparatus of the semicircular canals of the frog. The ampullary DC potential, the vestibular nerve multiunit discharge, the nerve DC potential and the unitary EPSP activity were recorded in isolated semicircular canals of the frog (Rana esculenta L). The results demonstrated that perilymphatic microperfusion of streptomycin (0.1, 0.3, 1 and 3 mM) reduced both resting and mechanically evoked afferent discharge, while the response of the hair cells remains unchanged. Intracellular recordings from single afferent axons showed that the reduction of the afferent discharge was mainly due to a reduction of the amplitude, but not the frequency, of the EPSPs. These results indicate that streptomycin, when applied in the fluid bathing the synaptic pole of the sensory cells, can act as an antagonist of the vestibular afferent transmitter at the postsynaptic level.
authors.affiliation: Istituto di Fisiologia Generale, Università di Pavia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Zucca
authors.forename: R
authors.initials: R
authors.lastname: Vega
authors.forename: L
authors.initials: L
authors.lastname: Botta
authors.forename: M E
authors.initials: ME
authors.lastname: Pérez
authors.forename: P
authors.initials: P
authors.lastname: Valli
authors.forename: E
authors.initials: E
authors.lastname: Soto
mesh_terms: D000344:Afferent Pathways / Q000187:drug effects / Q000502:physiology; D000818:Animals; D004594:Electrophysiology; D005072:Evoked Potentials, Auditory / Q000187:drug effects; D066298:In Vitro Techniques; D010477:Perfusion; D010812:Physical Stimulation; D011893:Rana esculenta; D012665:Semicircular Canals / Q000187:drug effects* / Q000294:innervation / Q000502:physiology; D013307:Streptomycin / Q000494:pharmacology*; D013569:Synapses / Q000187:drug effects / Q000502:physiology
chemical_list: D013307:Streptomycin