Interleukin-5 modulates eosinophil accumulation in allergic guinea pig lung.
Based on its involvement in eosinophil biology, interleukin 5 (IL-5) may play a role in the pulmonary eosinophilia associated with allergic reactions. We have examined that hypothesis using a neutralizing antibody to IL-5 in ovalbumin-sensitized guinea pigs challenged with aerosolized antigen. The extent of eosinophilia has been quantitated in bronchoalveolar lavage (BAL) and by histologic evaluation of lung tissue sections. Acute intraperitoneal administration of a rat IgG, monoclonal antibody to murine IL-5 derived from TRFK-5 cells prevented lung and BAL eosinophilia in a dose-dependent fashion at and above 10 micrograms per guinea pig. Treatment with either an experimentally irrelevant, isotype-matched antibody from GL113 cells or with heat-denatured IL-5 antibody was without effect. These studies demonstrate the importance of IL-5 to pulmonary eosinophilia in challenged, allergic guinea pigs.
Gulbenkian
A R
AR
Schering-Plough Research, Allergy and Cell Biology, Bloomfield, New Jersey 07003.
Egan
R W
RW
Fernandez
X
X
Jones
H
H
Kreutner
W
W
Kung
T
T
Payvandi
F
F
Sullivan
L
L
Zurcher
J A
JA
Watnick
A S
AS
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D001992:Bronchoalveolar Lavage Fluid / Q000473:pathology; D004804:Eosinophils / Q000473:pathology*; D006168:Guinea Pigs; D007114:Immunization; D015848:Interleukin-5 / Q000276:immunology / Q000494:pharmacology*; D008168:Lung / Q000473:pathology*; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000473:pathology*
D000906:Antibodies; D015848:Interleukin-5; D010047:Ovalbumin