Enhanced chemiluminescence in the oxidation of luminol and an isoluminol cortisol conjugate by hydrogen peroxide in reversed micelles.
The chemiluminescent oxidation of luminol and an isoluminol cortisol conjugate (ABI-COR) by hydrogen peroxide has been studied in cetyltrimethylammonium bromide (CTAB) reversed micelles in octane-chloroform (1:1). The maximum chemiluminescence intensity of both compounds is dependent on the initial concentrations of the H2O2 and substrates, the pH value of the micelle polar phase and the H2O/CTAB ratio. The optimum pH ranged from 8.5 to 9.5. Under comparable conditions, the chemiluminescence intensity for luminol was 15-fold higher than for the ABI-COR conjugate. A mechanism of oxidation of the substrates in reversed micelles is proposed and the possible mechanisms of inhibition by the substrate and oxidant is discussed.
Metelitza
D I
DI
Institute of Bioorganic Chemistry, BSSR Academy of Sciences, Minsk, USSR.
Eryomin
A N
AN
Shibaev
V A
VA
D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D002725:Chloroform / Q000737:chemistry; D006854:Hydrocortisone / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008165:Luminol / Q000031:analogs & derivatives* / Q000737:chemistry*; D008823:Micelles; D009795:Octanes / Q000737:chemistry; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence
D002593:Cetrimonium Compounds; D008823:Micelles; D009795:Octanes; C032487:isoluminol; D008165:Luminol; D002725:Chloroform; D006861:Hydrogen Peroxide; D006854:Hydrocortisone; C026728:octane; D000077286:Cetrimonium