title: Glucose utilization and insulin action in adult rats submitted to prolonged food restriction.
abstract: Glucose tolerance and insulin effects on glucose production and utilization by various tissues were studied in 70-day-old anesthetized rats submitted to food restriction from the fetal stage. Basal and glucose-induced plasma insulin levels were reduced in food-restricted rats without alterations in glucose tolerance. Insulin action was quantified by using the euglycemic-hyperinsulinemic clamp technique. Glucose turnover rates were measured by using D-[6-3H]glucose. Exogenous insulin failed to decrease glucose production in food-restricted rats. Weight-related whole body glucose utilization was higher in restricted rats than in controls both in the basal (21.9 +/- 0.7 vs. 9.4 +/- 0.6 mg.min-1.kg-1) and hyperinsulinemic states (37.5 +/- 1.1 vs. 14.0 +/- 1.2 mg.min-1.kg-1). Local glucose utilization by peripheral tissues was estimated by a 2-deoxy-D-[1-3H]glucose technique. In both basal and hyperinsulinemic conditions glucose utilization was increased in various adipose and muscle tissues of the food-restricted rats as compared with the controls. Thus we conclude that food restriction leads to an increase in the insulin-mediated glucose uptake by various peripheral tissues and to insulin resistance in the liver.
authors.affiliation: Instituto de Bioquímica, Centro Mixto: Facultad de Farmacia-Consejo Superior Investigaciones Científicas, Universidad Complutense, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Escrivá
authors.forename: C
authors.initials: C
authors.lastname: Rodríguez
authors.forename: J
authors.initials: J
authors.lastname: Cacho
authors.forename: C
authors.initials: C
authors.lastname: Alvarez
authors.forename: B
authors.initials: B
authors.lastname: Portha
authors.forename: A M
authors.initials: AM
authors.lastname: Pascual-Leone
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D007328:Insulin; D006003:Glycogen; D005947:Glucose