Regional alterations in intestinal sucrase expression in streptozocin-treated chronically diabetic rats.
In ad libitum-fed diabetic rats, sucrase specific activities in jejunum and ileum were significantly increased two- to threefold compared to controls, a response unaltered by pair-feeding. Gradients of sucrase activities along the ileal villus-to-crypt axis were readily measured in crypt regions in diabetic, but not in nondiabetic, rats. Changes in sucrase activities were commensurate with increases in sucrase immunoreactivity and not a result of altered functional activity. Insulin treatment reversed these effects, although insulin-deficiency, studied in food-deprived, nondiabetic rats, did not affect sucrase expression. We conclude that chronic diabetes significantly stimulates sucrase expression along the proximal-to-distal and villus-to-crypt axes of rat small intestine. In ileum, these changes suggest marked alterations in phenotypic development of enterocytes along the villus-to-crypt axis. Alterations in sucrase expression do not appear to correlate with insulin states and are not a consequence of altered functional activity.
Hoffman
L R
LR
Department of Medicine, University of Chicago, Illinois 60637.
Yen
S
S
Chang
E B
EB
D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000201:enzymology*; D005508:Food Deprivation / Q000502:physiology; D006963:Hyperphagia / Q000503:physiopathology; D007082:Ileum / Q000201:enzymology*; D007328:Insulin / Q000502:physiology / Q000627:therapeutic use; D007413:Intestinal Mucosa / Q000201:enzymology; D007583:Jejunum / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013393:Sucrase / Q000378:metabolism*
D007328:Insulin; D013393:Sucrase