title: Estimation and separation of N-acetyl-beta-D-glucosaminidase isoenzymes in urine.
abstract: N-acetyl-beta-D-glucosaminidase is a lysosomal enzyme made up of two isoenzymes (A and B). It has been used extensively as a marker for kidney damage. However, its estimation in urine has not been standardized. We have established a method for the estimation and separation of NAG isoenzymes by ion-exchange chromatography. In 19 experiments done so far, this method has given reproducible results. The significance of this method is that with a single experiment, one can estimate total as well as individual isoenzyme activity. Furthermore, urine constituents do not appear to interfere in this assay.
authors.affiliation: Department of Biochemistry, Aga Khan University Medical College, Karachi.
authors.forename: M H
authors.initials: MH
authors.lastname: Javed
authors.forename: A N
authors.initials: AN
authors.lastname: Hussain
authors.forename: M A
authors.initials: MA
authors.lastname: Waqar
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D007527:Isoenzymes / Q000652:urine*; D007669:Kidney Calculi / Q000652:urine*
chemical_list: D007527:Isoenzymes; D000118:Acetylglucosaminidase