Reduction in the ADP release from shear-stressed red blood cells by fish oil administration.
Fish oil concentrate (5.4 g/day) was administered to 8 young male volunteers and to 7 middle-aged male volunteers for 1 week. ADP released into the supernatant of red blood cell (RBC) suspension by a shear stress of 375/sec for 3 min was measured before and after fish oil administration. Before the administration the ADP release from shear-stressed RBCs in the middle-aged group was significantly higher than in the young group. After the administration the ADP release was reduced significantly in the young group (by 46%, p less than 0.001) and nonsignificantly in the middle-aged group (by 54%, p = 0.09). If the two groups were combined, the reduction was also significant (by 50%, p less than 0.005). This reduction was significantly correlated with the improvement of RBC filterability after fish oil administration (n = 13, r = 0.62, p less than 0.05). We suggest that the reduction of the ADP release from shear-stressed RBCs is one of the mechanisms of action of eicosapentaenoic acid against thrombotic disease.
Kobayashi
S
S
Department of Pharmacy, University Hospital, Tokyo Medical and Dental University.
Hamazaki
T
T
Sawazaki
S
S
Nakamura
H
H
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000328:Adult; D000367:Age Factors; D001809:Blood Viscosity / Q000187:drug effects; D004907:Erythrocyte Deformability / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005395:Fish Oils / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008563:Membrane Lipids / Q000032:analysis; D008875:Middle Aged; D013927:Thrombosis / Q000517:prevention & control
D005395:Fish Oils; D008563:Membrane Lipids; D000244:Adenosine Diphosphate