title: Conformational analysis of an opioid peptide in solvent media that mimic cytoplasm viscosity.
abstract: Many neuropeptides exert their action between the presynaptic vesicles and postsynaptic transmembrane receptors, crossing different layers of specialized cytoplasm. Biomimetic media usually employed to study bioactive peptides do not reproduce the physico chemical environment of cytoplasm--in particular, the high viscosity of this biological fluid. Here we describe a conformational study of a delta-selective opioid peptide, deltorphin I, at variable temperatures in several biocompatible media characterized by varying values of viscosity and dielectric constant. It was found that only viscosity, among these parameters, induces ordered conformations; that is, it acts as a conformational sieve. This finding suggests that the high viscosity of the intersynaptic fluid contributes, in addition to the membrane catalysis proposed by Schwyzer, in overcoming the so-called entropic barrier to the transition state of peptide-receptor interaction by selecting ordered conformations prior to receptor interaction. The folded conformer found in the 80:20 (v:v) DMSOd6/H2O cryoprotective mixture at 265 K has a shape consistent with those of rigid nonpeptidic opiates.
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Napoli Federico II, Italy.
authors.forename: P A
authors.initials: PA
authors.lastname: Temussi
authors.forename: D
authors.initials: D
authors.lastname: Picone
authors.forename: G
authors.initials: G
authors.lastname: Saviano
authors.forename: P
authors.initials: P
authors.lastname: Amodeo
authors.forename: A
authors.initials: A
authors.lastname: Motta
authors.forename: T
authors.initials: T
authors.lastname: Tancredi
authors.forename: S
authors.initials: S
authors.lastname: Salvadori
authors.forename: R
authors.initials: R
authors.lastname: Tomatis
mesh_terms: D000595:Amino Acid Sequence; D003593:Cytoplasm / Q000737:chemistry*; D004723:Endorphins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry*; D011487:Protein Conformation; D012997:Solvents; D014783:Viscosity
chemical_list: D004723:Endorphins; D009842:Oligopeptides; D012997:Solvents; C060208:deltorphin I, Ala(2)-