N-unsubstituted glucosamine residues and other modifications in murein of the obligatory chemolithotroph Thiobacillus neapolitanus.
Purified murein from Thiobacillus neapolitanus was poorly digested by lysozyme. It's sensitivity to the enzyme greatly increased after N-acetylation. The murein was found to contain 30 to 35% glucosamine residues lacking N-acetyl groups. It also contained phosphomuramic acid. Further modifications included amidation of diaminopimelic acid in the peptide side chains and a low alanine content. None of these modifications were found in the murein of another sulphur bacterium, Thiobacillus versutus.
Baj
J
J
Institute of Microbiology, University of Warsaw.
Grabowska
I
I
Markiewicz
Z
Z
D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D006595:Hexosamines / Q000032:analysis; D066298:In Vitro Techniques; D009112:Muramic Acids / Q000032:analysis; D009113:Muramidase / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry* / Q000302:isolation & purification; D013855:Thiobacillus / Q000378:metabolism*
D000596:Amino Acids; D000606:Amino Sugars; D006595:Hexosamines; D009112:Muramic Acids; D010457:Peptidoglycan; D009113:Muramidase