title: Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups.
abstract: A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.
authors.affiliation: Department of Chemistry, University of Edinburgh, UK.
authors.forename: C
authors.initials: C
authors.lastname: MacKellar
authors.forename: D
authors.initials: D
authors.lastname: Graham
authors.forename: D W
authors.initials: DW
authors.lastname: Will
authors.forename: S
authors.initials: S
authors.lastname: Burgess
authors.forename: T
authors.initials: T
authors.lastname: Brown
mesh_terms: D001483:Base Sequence; D002784:Cholesterol / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D016655:HIV Core Protein p24 / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000737:chemistry; D013696:Temperature; D013873:Thionucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D016655:HIV Core Protein p24; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; D002784:Cholesterol