title: Plasma cell proliferation in the chicken harderian gland.
abstract: Studies to examine the percentages of proliferating plasma cells (PPC) in the Harderian gland (HG) were carried out in chicks between 5 and 12 weeks of age. Two methods, 5-bromo-2'-deoxyuridine (BrdUrd) incorporation into DNA and flow cytometric analysis of propidium iodide (PI) stained cells, were employed in control and emetine dihydrochloride treated birds. Flow cytometric analysis of PI stained cells showed the percentages of plasma cells in S phase were highest between 6 and 8 weeks of age. After this period of time, the number of S phase plasma cells decreased and remained low through 12 weeks of age. The lowest percentages of plasma cells in G0 + G1 were found at 6 and 8 weeks of age, and all ages had equal percentages of plasma cells in G2 + M phase. After administration of the protein synthesis inhibitor emetine dihydrochloride a common pattern of plasma cell depletion and repopulation in the HG was observed. At 3 and 5 days post-treatment the plasma cell population in the gland decreased and by 7 days post-treatment repopulation of the gland with plasma cells had taken place. Anti-BrdUrd staining of frozen sections revealed that the number of PPC were decreased at 3 days after emetine treatment but were as high as, or higher than, controls at 5 and 7 days post-treatment. Flow cytometric analysis indicated that some birds were more severely affected by emetine. Namely, the percentages of plasma cells in S phase were lower at 3 and 5 days post-treatment. Even though most birds were severely affected by emetine treatment during the experiments, they possessed a cell population with the proliferative capacity to quickly repopulate the HG by 7 days post-emetine treatment.
authors.affiliation: Department of Poultry Science, Clemson University, South Carolina 29634-0379.
authors.forename: M L
authors.initials: ML
authors.lastname: Savage
authors.forename: I
authors.initials: I
authors.lastname: Oláh
authors.forename: T R
authors.initials: TR
authors.lastname: Scott
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000166:cytology* / Q000187:drug effects; D001973:Bromodeoxyuridine; D002455:Cell Division; D002645:Chickens; D004640:Emetine / Q000494:pharmacology; D005434:Flow Cytometry; D006243:Harderian Gland / Q000098:blood supply*; D007124:Immunoenzyme Techniques
chemical_list: D001973:Bromodeoxyuridine; D004640:Emetine