Novel model for study of human sperm chemotaxis.
OBJECTIVE
To develop and test an adequate method for the study of sperm chemotaxis.


METHODS
A model based on a double-chamber design and a matrix of 0.5% hyaluronic acid was used to test the response of human sperm to chemotactic signals from follicular fluid (FF).


RESULTS
A plateau of migration was reached at 10 minutes of incubation; under this condition, 70% of sperm migrated toward chambers with FF, less than 20% to the opposite side, and the rest remained at the site of application. Intra-assay and interassay coefficients of variation using pools of FFs were 17.2% and 23%, respectively.


CONCLUSIONS
These data confirm previous findings and support the use of this novel model for the study of chemotaxis on human gametes.
Villanueva-Díaz
C
C
Instituto Nacional de Perinatología, México City, México.
Arias-Martínez
J
J
Bustos-López
H
H
Vadillo-Ortega
F
F
D002633:Chemotaxis; D004958:Estradiol / Q000032:analysis; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D007700:Kinetics; D008297:Male; D011374:Progesterone / Q000032:analysis; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
D011374:Progesterone; D004958:Estradiol