Sodium butyrate selectively induces transcription of promoters adjacent to the MoMSV viral enhancer.
The long terminal repeat region of the Moloney murine sarcoma virus (MoMSV) was cloned upstream from the Chinese hamster ovary adenine phosphoribosyltransferase (APRT)-encoding gene (APRT) in order to enhance synthesis of the APRT protein. The replacement of the native promoter with the viral enhancer-promoter increased the enzymatic activity of APRT two- to threefold. Addition of sodium butyrate (NaBu) to the cell growth medium induced APRT activity ten- to 20-fold above wild-type levels in both transient and stable transfectants. The introduction of the APRT native promoter between the MoMSV enhancer-promoter and structural gene reduced the magnitude of the NaBu response. The bacterial cat gene was also stimulated by NaBu when linked to the viral enhancer-promoter. No NaBu response was found in constructs lacking the MoMSV enhancer region. Northern analysis and nuclear run-on experiments indicated that NaBu enhanced transcription of APRT mRNA in both transiently and stably transfected cells, but not in cells inhibited by cycloheximide. Thus, a butyrate-response element (BRE) is associated with the MoMSV enhancer and the action of the MoMSV BRE is promoter-dependent.
Yeivin
A
A
Department of Biology, Indiana University, Bloomington 47405.
Tang
D C
DC
Taylor
M W
MW
D000228:Adenine Phosphoribosyltransferase / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D006224:Cricetinae; D004742:Enhancer Elements, Genetic / Q000235:genetics; D008980:Moloney murine sarcoma virus / Q000187:drug effects* / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D012333:RNA, Messenger / Q000187:drug effects; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection
D002087:Butyrates; D012333:RNA, Messenger; D020148:Butyric Acid; D000228:Adenine Phosphoribosyltransferase