title: Purification of human leucocyte DNA: proteinase K is not necessary.
abstract: A rapid nontoxic method for the purification of DNA from human leucocytes is described. Preliminary experiments which tested different methods of DNA purification indicated that digestion of proteins with proteinase K was unnecessary. This led to the development of a simple procedure involving lysis of the cells in SDS followed by extraction with 6 M NaCl. The method described overcomes the requirement for lengthy incubations in the presence of expensive proteinase K and subsequent extraction with toxic chemicals.
authors.affiliation: Victorian Institute of Forensic Pathology, South Melbourne, Australia.
authors.forename: A M
authors.initials: AM
authors.lastname: Douglas
authors.forename: A M
authors.initials: AM
authors.lastname: Georgalis
authors.forename: L R
authors.initials: LR
authors.lastname: Benton
authors.forename: K L
authors.initials: KL
authors.lastname: Canavan
authors.forename: B A
authors.initials: BA
authors.lastname: Atchison
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D019286:Endopeptidase K; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D012697:Serine Endopeptidases
chemical_list: D004247:DNA; D012697:Serine Endopeptidases; D019286:Endopeptidase K