Comparison of atropine pre- and post-treatment in ganglion neurons exposed to soman.
The prevention and reversal of the effects of soman (pinacoloxymethylphosporyl fluoride) have been examined on the electrical properties of sympathetic ganglion neurons in vitro from the adult bullfrog Rana catesbeiana. Atropine (10 microM) pre-treatment (before soman) blocked the soman-induced decrease in the membrane potential, membrane resistance, and afterhyperpolarization (AHP) duration. Atropine post-treatment (after soman exposure) restored the soman-induced decrease in the membrane potential but was ineffective in reversing either the membrane resistance or the duration of the AHP. These results demonstrate: 1) that the effects of soman on the electrical properties of these neurons are mediated by the activation of muscarinic receptors, 2) that following receptor activation different cellular mechanisms may be involved in the regulation of the electrical properties of the neuron, and 3) pre-treatment rather than post-treatment with atropine is more effective in blocking these direct effects of soman. These results are discussed in reference to the increased effectiveness of atropine pretreatment in the protocols for soman-induced neurotoxicity.
Heppner
T J
TJ
Department of Anatomy and Neurobiology, University of Vermont, College of Medicine, Burlington 05405.
Fiekers
J F
JF
D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D005724:Ganglia / Q000166:cytology* / Q000187:drug effects; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D011892:Rana catesbeiana; D012999:Soman / Q000037:antagonists & inhibitors* / Q000633:toxicity
D001285:Atropine; D012999:Soman