title: The AT2 subtype of the angiotensin II receptors has differential sensitivity to dithiothreitol in specific brain nuclei of young rats.
abstract: We studied the effect of the sulfhydryl reducing agent dithiothreitol on the binding of the angiotensin II agonist [125I][Sar1]-angiotensin II to AT2 receptors in selected brain areas of young (2-week-old) rats. In the inferior olive and the hypoglossal nucleus, angiotensin II binding to AT2 receptors was insensitive to 5 mM dithiothreitol. Conversely, in the ventral and mediodorsal thalamic, medial geniculate, and oculomotor nuclei, the superior colliculus and the cerebellar cortex, incubation with 5 mM dithiothreitol significantly decreased angiotensin II binding to AT2 receptors to about 40% of control. These data suggest that brain AT2 receptors are heterogeneous with respect to their sensitivity to sulfhydryl reducing agents.
authors.affiliation: Section on Pharmacology, National Institute of Mental Health, Bethesda, MD 20892.
authors.forename: K
authors.initials: K
authors.lastname: Tsutsumi
authors.forename: S
authors.initials: S
authors.lastname: Zorad
authors.forename: J M
authors.initials: JM
authors.lastname: Saavedra
mesh_terms: D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology / Q000254:growth & development; D001923:Brain Chemistry / Q000187:drug effects*; D004229:Dithiothreitol / Q000494:pharmacology*; D005260:Female; D007457:Iodine Radioisotopes; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000187:drug effects / Q000378:metabolism*
chemical_list: D007457:Iodine Radioisotopes; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D004229:Dithiothreitol