title: Comparison of a novel thin-layer chromatographic-fluorescence detection method with a spectrofluorometric method for the determination of 7-hydroxycoumarin in human urine.
abstract: A novel method for the determination of 7-hydroxycoumarin in human urine which combines thin-layer chromatography (TLC) with fluorescence detection (FD) has been devised. The limit of detection (1 ng/ml) enables determination of 7-hydroxycoumarin after both administration of coumarin and environmental exposure to this fragrance material. When compared to a spectrofluorometric method of analysis, the TLC-FD method proved to be more selective for the analysis of 7-hydroxycoumarin in human urine.
authors.affiliation: Department of Pharmacological Sciences, University of Newcastle upon Tyne, UK.
authors.forename: S
authors.initials: S
authors.lastname: Cholerton
authors.forename: M E
authors.initials: ME
authors.lastname: Idle
authors.forename: A
authors.initials: A
authors.lastname: Vas
authors.forename: F J
authors.initials: FJ
authors.lastname: Gonzalez
authors.forename: J R
authors.initials: JR
authors.lastname: Idle
mesh_terms: D000284:Administration, Oral; D002855:Chromatography, Thin Layer / Q000379:methods*; D003374:Coumarins / Q000008:administration & dosage; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014468:Umbelliferones / Q000652:urine*
chemical_list: D003374:Coumarins; D014468:Umbelliferones; C030123:coumarin