title: Synthesis of a gene encoding bovine substance P precursors and its expression in Escherichia coli.
abstract: Using an efficient Escherichia coli expression system, we have been able to obtain the precursor of substance P, alpha-preprotachykinin (alpha PPT). The alpha PPT protein is produced in E. coli as a fusion to beta-galactosidase, and accumulates in the cytoplasm as insoluble inclusion bodies. We also produced protachykinin (alpha PT), i.e., alpha PPT without a signal peptide. Further purification and characterization of the alpha PPT and alpha PT polypeptides strongly suggest that fully purified products can be obtained using our procedures.
authors.affiliation: Laboratory of Genetic Engineering, Sieradz, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Hrabec
authors.forename: Z
authors.initials: Z
authors.lastname: Hrabec
authors.forename: G
authors.initials: G
authors.lastname: Płucienniczak
authors.forename: G
authors.initials: G
authors.lastname: Skorupa
authors.forename: A
authors.initials: A
authors.lastname: Szczepanek
authors.forename: A
authors.initials: A
authors.lastname: Płucienniczak
authors.forename: B
authors.initials: B
authors.lastname: Uznański
authors.forename: A
authors.initials: A
authors.lastname: Wilk
authors.forename: A
authors.initials: A
authors.lastname: Okruszek
authors.forename: M
authors.initials: M
authors.lastname: Koziołkiewicz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005813:Genes, Synthetic / Q000235:genetics; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015320:Tachykinins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
chemical_list: D011498:Protein Precursors; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D015320:Tachykinins; C044206:preprotachykinin; C054618:protachykinin