Amino acid incorporation in cell cultures from eyes with pseudo-exfoliation material.
Conjunctival tissue was removed from eyes with and without pseudo-exfoliation material during cataract surgery. Cells derived from these samples were maintained in vitro. Confluent cultures were examined by transmission electron microscopy, and non-confluent as well as confluent cultures were examined for their ability to incorporate 3H-leucine and 3H-proline into TCA-precipitated material. Cells derived from both types of samples showed deposition of extracellular matrix, and a similar incorporation of the two amino acids. It is concluded that in vitro studies allow examination of basic metabolic patterns under varying conditions, and also a search for possible differences in such functions between tissues with and without production of pseudo-exfoliation material.
Nicolaissen
B
B
Department of Ophthalmology, Ullevål Hospital, University of Oslo, Norway.
Ringvold
A
A
Naess
O
O
D000869:Anterior Eye Segment / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D003229:Conjunctival Diseases / Q000378:metabolism / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D005128:Eye Diseases / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007930:Leucine / Q000378:metabolism*; D011392:Proline / Q000378:metabolism*
D011392:Proline; D007930:Leucine