Modification of the clastogenic activity of X-ray and 6-mercaptopurine in mice by prefeeding with vitamins C and E.
The effect of a 30-d pretreatment with vitamin C (L-ascorbic acid) in the drinking water and vitamin E (all-rac-alpha-tocopherol) in the diet on the clastogenic activity induced by X-rays and 6-mercaptopurine was investigated in female ICR/Jcl mice by the bone-marrow micronucleus test. Prefeeding with vitamin E-deficient diets led to a significant decrease in serum vitamin E concentration and to an enhancement of micronucleus formation by X-rays in bone marrow cells. Although dietary supplementation with vitamin E significantly increased the vitamin E concentration in serum, it did not affect the frequency of X-ray-induced micronuclei. Treatment with a high level of vitamin C in drinking water was effective in protecting against micronucleus formation by X-rays. The increase in micronucleus frequency in the vitamin E-deficient mice compared with the mice fed vitamin E-normal diets was no longer observed when a high level of vitamin C in drinking water was given simultaneously. The most efficient protective action against X-rays was observed when vitamin E-supplemented diets and a high level of vitamin C in drinking water were used together as a pretreatment. Any combination of the vitamins did not affect the micronucleus induction by 6-mercaptopurine.
Odagiri
Y
Y
Department of Public Health, Saitama Medical School, Japan.
Karube
T
T
Katayama
H
H
Takemoto
K
K
D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000097:blood / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D005260:Female; D015122:Mercaptopurine / Q000037:antagonists & inhibitors* / Q000633:toxicity; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D014810:Vitamin E / Q000008:administration & dosage / Q000097:blood / Q000627:therapeutic use*
D014810:Vitamin E; D015122:Mercaptopurine; D001205:Ascorbic Acid