A convenient method for the preparation of a variety of 13C-substituted D-fructose phosphates using readily available enzymes of the glycolytic pathway.
Methods are presented for the preparation of a variety of D-fructose phosphates, 13C-substituted at any single carbon site or at any two symmetrically disposed carbon sites, from either 13C-substituted pyruvate or L-alanine. It is demonstrated that millimole quantities of product can be obtained in good yield following a "one-pot" incubation of 13C-substituted precursors with commercially available enzymes and cofactors of the glycolytic pathway. Since it has previously been shown that a wide variety of aldehydes serve as acceptable substrates for the final rabbit muscle aldolase-catalyzed condensation step, the method can potentially be applied to prepare a wide variety of 13C-substituted sugars and sugar phosphates.
Kuan
K T
KT
Department of Chemistry, University of Texas, Dallas, Richardson 75083-0688.
Weber
D S
DS
Sottile
L
L
Goux
W J
WJ
D000409:Alanine / Q000378:metabolism; D002247:Carbon Isotopes; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005635:Fructosediphosphates / Q000096:biosynthesis*; D005636:Fructosephosphates / Q000096:biosynthesis*; D005942:Gluconates / Q000378:metabolism; D005947:Glucose / Q000096:biosynthesis; D006019:Glycolysis / Q000502:physiology*; D007553:Isotope Labeling; D011773:Pyruvates / Q000378:metabolism; D013237:Stereoisomerism; D014305:Triose-Phosphate Isomerase / Q000037:antagonists & inhibitors
D002247:Carbon Isotopes; D005635:Fructosediphosphates; D005636:Fructosephosphates; D005942:Gluconates; D011773:Pyruvates; D005634:Fructose-Bisphosphate Aldolase; D014305:Triose-Phosphate Isomerase; D005947:Glucose; D000409:Alanine; C030691:gluconic acid