title: Binding of low concentration of peptide to H-2Kd produced in insect cells requires mouse beta 2-microglobulin co-expression.
abstract: A recombinant baculovirus expressing the murine class I MHC heavy chain H-2Kd cDNA under the transcriptional control of Autografa californica nuclear polyhedrosis virus (AcNPV) polyhedrin promoter has been isolated and used to infect Sf9 lepidopteran cells either alone or in association with a previously isolated virus expressing mouse beta 2-microglobulina (beta 2-ma). When infected with the heavy chain-encoding virus alone, H-2Kd was produced in a beta 2-m-free conformation detected on the surface of infected cells by conformation-independent antibodies. When Sf9 cells were co-infected with both viruses, approximately 10% of the heavy chain pool was engaged in the formation of native heterodimeric MHC class I molecules, which were glycosylated and transported to the cell surface as demonstrated by radio-binding experiments and flow cytometry. The assembly of the recombinant class I molecule was dependent on peptide, since heterodimer formation was brought about by H-2Kd-specific peptide ligands both in vivo, upon incubation with dually infected cells, and in vitro, in cell-free detergent extracts. In addition, a change in heavy chain conformation was brought about upon incubation with high concentrations (100 microM) of an H-2Kd-restricted octapeptide epitope from Plasmodium berghei. Furthermore, using low concentrations (3 nM) of a photoaffinity label derivative of this peptide, we show direct binding to cells co-expressing class I heavy chain and mouse beta 2-m but not to cells expressing free heavy chain only.
authors.affiliation: Unité de Biologie Moléculaire du Gène, INSERM U 277, Institut Pasteur, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Godeau
authors.forename: J L
authors.initials: JL
authors.lastname: Casanova
authors.forename: I F
authors.initials: IF
authors.lastname: Luescher
authors.forename: K D
authors.initials: KD
authors.lastname: Fairchild
authors.forename: C
authors.initials: C
authors.lastname: Delarbre
authors.forename: C
authors.initials: C
authors.lastname: Saucier
authors.forename: G
authors.initials: G
authors.lastname: Gachelin
authors.forename: P
authors.initials: P
authors.lastname: Kourilsky
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000937:Antigen-Antibody Reactions / Q000502:physiology*; D016367:Baculoviridae; D005786:Gene Expression Regulation; D006031:Glycosylation; D006183:H-2 Antigens / Q000276:immunology*; D015151:Immunoblotting; D007915:Lepidoptera; D051379:Mice; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology; D014170:Transformation, Genetic; D001613:beta 2-Microglobulin / Q000502:physiology*
chemical_list: D006183:H-2 Antigens; C024755:H-2K(K) antigen; D010455:Peptides; D011994:Recombinant Proteins; D001613:beta 2-Microglobulin