Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response.
Treatment of bean or soybean cells with fungal elicitor or glutathione causes a rapid insolubilization of preexisting (hydroxy)proline-rich structural proteins in the cell wall. This insolubilization, which involves H2O2-mediated oxidative cross-linking, is initiated within 2 min and is complete within 10 min under optimal conditions, and hence, precedes the expression of transcription-dependent defenses. Cross-linking is also under developmental control during hypocotyl growth and in tissues subject to mechanical stress such as the stem-petiole junction. Stimulus-dependent oxidative cross-linking of wall structural proteins is a novel site of cellular regulation with potentially important functions in cell maturation and toughening of cell walls in the initial stages of plant defense.
Bradley
D J
DJ
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037.
Kjellbom
P
P
Lamb
C J
CJ
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002473:Cell Wall / Q000737:chemistry*; D005978:Glutathione / Q000494:pharmacology; D008565:Membrane Proteins / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009024:Morphogenesis; D063245:Plant Development; D011392:Proline; D013025:Glycine max / Q000737:chemistry / Q000166:cytology
D008565:Membrane Proteins; D011392:Proline; D005978:Glutathione