Isolation and characterization of alpha 1-acid glycoprotein from horses, and its evaluation as an acute-phase reactive protein in horses.
Equine alpha 1-acid glycoprotein (alpha 1AG) was isolated from equine serum by successive ammonium precipitation, anion- and cation-exchange chromatographies, and gel filtration. Purified equine alpha 1AG had a molecular weight of 46,000 +/- 1,000, and contained 31.4% carbohydrate. Gel isoelectric focusing revealed an isoelectric point range of 2.8 to 3.7. With immunoelectrophoresis, it was found that alpha 1AG migrated to the alpha 1-globulin region. Single radial immunodiffusion was used for quantitative measurement of alpha 1AG in equine serum. In clinically normal foals, serum alpha 1AG was undetectable (less than or equal to 20 micrograms/ml) in less than or equal to 7-day-old foals, but was detected by 14 days. The alpha 1AG concentration (mean +/- SD) increased to reach mean adult values of 99.23 +/- 26.90 micrograms/ml by 1 year of age. The alpha 1AG concentration in pregnant mares decreased at 2 to 3 months before parturition, then gradually increased until 1 day after parturition, when a brief decrease was observed. The concentration increased again at 2 weeks after foaling, then a decrease was observed, after which the alpha 1AG concentration increased again by 2 to 4 months after parturition. The concentration of serum alpha 1AG quickly rose to peak values 2 to 3 days after castration and jejunojejunostomy in adult horses, returning to baseline values by 14 to 28 days after surgery. The alpha 1AG was concluded to be an acute-phase reactive protein in horses.
Taira
T
T
Department of Veterinary Surgery, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Fujinaga
T
T
Tamura
K
K
Izumi
M
M
Itoh
H
H
Tsunoda
N
N
Yamashita
K
K
Okumura
M
M
Mizuno
S
S
D000210:Acute-Phase Reaction / Q000097:blood / Q000662:veterinary*; D000375:Aging / Q000097:blood; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000831:Animals, Newborn; D005260:Female; D006734:Horse Diseases / Q000097:blood*; D006736:Horses; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007249:Inflammation / Q000097:blood / Q000662:veterinary*; D007525:Isoelectric Focusing; D008297:Male; D009961:Orosomucoid / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000097:blood*
D000596:Amino Acids; D009961:Orosomucoid