[The protective effect of apolipoprotein C1 on endothelial cells injured by low density lipoprotein in vitro].
High serum low density lipoprotein (LDL) level is known injurious to endothelial cell (EC) and that high density lipoprotein (HDL) protects EC from such injury. The protective effect of HDL and apolipoprotein C1 (apo C1) on the endothelial cells (EC) isolated originally from human umbilical vein and injured by LDL was studied morphologically with phase-contrast and transmission electron microscope. Additionally, their functional changes were detected by measuring the amount of lactate dehydrogenase (LDH) and 6-keto-prostaglandin F1 alpha (PGF1 alpha) released. The EC were divided into four groups: control, HDL+LDL, apo C1 + IDL and LDL group. EC after being injured by LDL showed cell contraction, increased release of LDH and decreased secretion of PGF1. Anyhow, they would be left normal on morphology, LDH release and PGF1 alpha synthesis if HDL or apo C1 had been added to the culture media before LDL injury. The results indicated that both HDL and apo C1 can resist the injurious effect of LDL on the cultured EC.
Liu
Q H
QH
Beijing Institute of Geriatrics, Ministry of public Health, P. R. China.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D053302:Apolipoprotein C-I; D001056:Apolipoproteins C / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors*
D053302:Apolipoprotein C-I; D001056:Apolipoproteins C; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D015121:6-Ketoprostaglandin F1 alpha; D007770:L-Lactate Dehydrogenase