Neurohormonal mechanism of pancreatic exocrine secretion stimulated by sodium oleate and L-tryptophan in dogs.
In the present investigation, we have studied the effect of atropine on the pancreatic secretion stimulated by intraduodenal administration of either sodium oleate or exogenous cholecystokinin (CCK). In four dogs prepared with gastric and Thomas duodenal cannulas, pancreatic juice was collected for measurement of volume, bicarbonate, and protein output, and peripheral venous blood samples were obtained for radioimmunoassay of both secretin and CCK. Volume, bicarbonate, and protein output of the pancreatic juice increased significantly in response to sodium oleate (1-4 mmol/h) in a dose-dependent manner. The increase in pancreatic secretion paralleled the increments in both plasma CCK and secretin. Atropine given intravenously suppressed completely both pancreatic secretion and release of CCK stimulated by sodium oleate, whereas the release of secretin was not affected. Pancreatic secretion was significantly increased in a dose-dependent manner by exogenous CCK octapeptide (CCK-8) at 16, 32, and 64 micrograms (14, 28, and 56 pmol).kg-1.h-1. Atropine inhibited protein output only partially, but it did not influence bicarbonate output. In five additional dogs, the effect of atropine on L-tryptophan-stimulated pancreatic secretion was studied. Interestingly, atropine failed to influence the CCK release and pancreatic secretion of volume and bicarbonate, except for protein secretion, which was significantly inhibited. It was shown previously that atropine inhibited significantly the pancreatic secretion of bicarbonate stimulated by secretin in physiological doses. Thus we conclude that the inhibition by atropine of the pancreatic exocrine secretion stimulated by sodium oleate is mediated by both suppression of CCK release and inhibition of action of secretin on the exocrine pancreas.(ABSTRACT TRUNCATED AT 250 WORDS)
Jo
Y H
YH
Genesee Hospital, University of Rochester School of Medicine and Dentistry, New York 14607.
Lee
Y L
YL
Lee
K Y
KY
Chang
T M
TM
Chey
W Y
WY
D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D002766:Cholecystokinin / Q000097:blood / Q000378:metabolism*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*; D010179:Pancreas / Q000378:metabolism*; D012633:Secretin / Q000097:blood / Q000378:metabolism*; D012844:Sincalide / Q000494:pharmacology; D014364:Tryptophan / Q000494:pharmacology*
D009829:Oleic Acids; D012633:Secretin; D019301:Oleic Acid; C013173:osteum; D001285:Atropine; D014364:Tryptophan; D002766:Cholecystokinin; D012844:Sincalide