Demonstration of multiple forms of bovine brain myristoyl CoA:protein N-myristoyl transferase.
Four distinct N-myristoyl transferase (NMT) activity peaks, designated I, II, III, and IV, were separated from the cytosolic fraction of bovine brain by DEAE-Sepharose column chromatography. Peaks I, II, III and IV were characterised biochemically with respect to substrate specificity: with cAMP-dependent protein kinase and pp60src derived peptides, and by their apparent molecular mass. The apparent molecular mass of peaks I, II, III and IV were 190 kDa, 224 kDa, 390 kDa and 76 kDa, respectively. These results indicate that bovine brain contains multiple forms of NMT.
King
M J
MJ
Department of Pathology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Sharma
R K
RK
D000217:Acyltransferases / Q000302:isolation & purification*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002852:Chromatography, Ion Exchange; D007527:Isoenzymes / Q000302:isolation & purification; D007700:Kinetics; D008970:Molecular Weight; D013379:Substrate Specificity
D007527:Isoenzymes; D000217:Acyltransferases; C050480:glycylpeptide N-tetradecanoyltransferase