Intranuclear topological distribution of HIV-1 trans-activators.
Subcellular localization of human immunodeficiency virus type I (HIV-1) Tat and Rev was examined using a confocal laser scanning microscope (CLSM). In transfected COS-7 cells, Tat resided exclusively in the perinocleolar region, while Rev infiltrated fully into the nucleoli. The chimeric Tat in which the nucleolar targeting signal was replaced by that of Rev, which retains trans-acting activity of Tat, remained still in the perinucleolar region as wild-type Tat. Perinucleolar distribution of Tat protein suggests the existence of a novel nucleolar architecture that affects transcription.
Miyazaki
Y
Y
Institute for Virus Research, Kyoto University, Japan.
Takamatsu
T
T
Nosaka
T
T
Fujita
S
S
Hatanaka
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000382:microbiology; D003593:Cytoplasm / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D015694:Gene Products, rev / Q000032:analysis*; D015696:Gene Products, tat / Q000032:analysis*; D015497:HIV-1 / Q000235:genetics*; D008969:Molecular Sequence Data; D014162:Transfection; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D015696:Gene Products, tat; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus