Occurrence of a Photosystem II polypeptide in non-photosynthetic membranes of cyanobacteria.
Cytoplasmic and thylakoid membranes have been purified from the cyanobacteria Anacystis nidulans R2 and Phormidium laminosum by sucrose density gradient centrifugation. Probing of Western blots of proteins from these purified membrane fractions with antibodies directed against the 33 kDa polypeptide of Photosystem II from pea indicates that this protein is present in both the thylakoid and cytoplasmic membranes, rather than just the thylakoid membranes. This has been confirmed by immunogold labelling of cells. Oxygen evolution assays have been used to show that the 33 kDa polypeptide is not assembled into a functional Photosystem II complex in the cytoplasmic membranes. This may be due to the absence of other Photosystem II components.
Smith
D
D
Department of Biochemistry, University of Cambridge, UK.
Bendall
D S
DS
Howe
C J
CJ
D001426:Bacterial Proteins; D015153:Blotting, Western; D002338:Carotenoids / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry*; D002734:Chlorophyll / Q000032:analysis; D000458:Cyanobacteria / Q000737:chemistry*; D006602:Hexosyltransferases; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000737:chemistry; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D002267:Muramoylpentapeptide Carboxypeptidase / Q000032:analysis; D010100:Oxygen / Q000378:metabolism; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D045322:Photosynthetic Reaction Center Complex Proteins / Q000032:analysis* / Q000378:metabolism; D010860:Pigments, Biological / Q000032:analysis
D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D046915:Penicillin-Binding Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D010860:Pigments, Biological; D002734:Chlorophyll; D002338:Carotenoids; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D010100:Oxygen