title: Positive and negative DNA elements of the Drosophila grimshawi s18 chorion gene assayed in Drosophila melanogaster.
abstract: Germ line transformation has been used to map the cis regulatory DNA elements responsible for the precise and evolutionarily stable developmental expression of the s18 chorion gene. Constructs containing chimeric combinations of Drosophila melanogaster and D. grimshawi DNA regions, as well as D. grimshawi sequences alone, can direct expression in the follicular epithelium, in an s18-specific temporal and spatial pattern. The results indicate that both positive and negative regulatory elements can function when transferred from D. grimshawi to D. melanogaster. The first ca. 100 bp of the 5'-flanking DNA region constitute a minimal, developmentally regulated promoter, expression of which is inhibited by the next 100-bp DNA segment and activated by positive elements located further upstream. Expression of the minimal promoter can also be enhanced by more distant chorion regulatory elements, provided the inhibitory DNA segment is absent.
authors.affiliation: Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
authors.forename: C
authors.initials: C
authors.lastname: Swimmer
authors.forename: H
authors.initials: H
authors.lastname: Kashevsky
authors.forename: G
authors.initials: G
authors.lastname: Mao
authors.forename: F C
authors.initials: FC
authors.lastname: Kafatos
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004330:Drosophila / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D007763:Lac Operon; D008969:Molecular Sequence Data; D014158:Transcription, Genetic
chemical_list: D004247:DNA