title: Metabolic effects of dietary stearic acid in mice: changes in the fatty acid composition of triglycerides and phospholipids in various tissues.
abstract: The fatty acid patterns of triglycerides and phospholipids extracted from adipose tissue, liver, heart, kidney, spleen, and lung of 3 groups of C57BL/6 mice were determined after feeding diets rich in palmitic acid (16:0) (high palmitic: 16:0 = 45.1% of total fatty acids), stearic acid (18:0) (high stearic: 18:0 = 42.9% of total fatty acids) and oleic acid (18:1) (high oleic: 18:1 = 79.7% of total fatty acids) for 9 months. Triglyceride content of adipose, liver, heart, kidney, lung and spleen tissues was significantly enriched in palmitic acid in mice fed the high palmitic diet (range among all tissues: 19.9% +/- 0.2% to 29.0% +/- 1.9% of total fatty acids) and in oleic acid in mice fed the high oleic diet (range 56.0% +/- 1.9% to 71.6% +/- 1.2%). The stearic acid content of organ triglycerides in mice fed the high stearic diet ranged from 3.7% +/- 0.3% to 10.8% +/- 1.2%; however, the content of oleic acid on this diet (range: 57.0% +/- 1.8% to 71.4% +/- 1.7%) was similar to the one observed in mice fed the high oleic diet. In all organs, phospholipids had a significantly higher percentage of stearic acid (range: 23.5% +/- 0.9% to 51.5% +/- 6.6%) than triglycerides, regardless of diet. To evaluate the production of oleate from stearate and palmitate, 2 groups of mice were fed the high palmitic and the high stearic diets for 1 week and then injected intravenously with [1-14C]palmitate and [1-14C]stearate and the amount of labelled oleate in liver triglycerides was measured.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas 75235-9052.
authors.forename: A
authors.initials: A
authors.lastname: Bonanome
authors.forename: M
authors.initials: M
authors.lastname: Bennett
authors.forename: S M
authors.initials: SM
authors.lastname: Grundy
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D004032:Diet; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000737:chemistry; D019301:Oleic Acid; D009829:Oleic Acids / Q000008:administration & dosage / Q000494:pharmacology; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000008:administration & dosage / Q000494:pharmacology; D010743:Phospholipids / Q000737:chemistry*; D013154:Spleen / Q000737:chemistry; D013229:Stearic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D014280:Triglycerides / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D010743:Phospholipids; D013229:Stearic Acids; D014280:Triglycerides; D019301:Oleic Acid; D019308:Palmitic Acid; C031183:stearic acid