Expression of pS2 gene in human breast cancer cell line MCF-7 is controlled by retinoic acid.
We established a simplified method for the quantitative measurement of pS2 mRNA using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. Expression of the pS2 gene, which is transcriptionally induced by estrogen in breast cancer cell line MCF-7 cells, can be repressed by retinoic acid (RA) in unstimulated cells. The suppressive effect of RA on pS2 mRNA was inhibited by cycloheximide.
Hirota
M
M
Department of Molecular Biology, Gifu Parmaceutical University, Japan.
Furukawa
Y
Y
Hayashi
K
K
D001943:Breast Neoplasms / Q000235:genetics*; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014212:Tretinoin; D003513:Cycloheximide