Arginine-vasopressin fragment 4-9 stimulates the acetylcholine release in hippocampus of freely-moving rats.
We examined the effects of arginine-vasopressin (AVP) C-terminal fragment 4-9, which facilitates learning and memory, on the extracellular acetylcholine (ACh) release in hippocampus of freely-moving rats using the microdialysis technique. Following administration of AVP4-9, p-Glu-Asn-Cys[Cys]-Pro-Arg-Gly-NH2, through the dialysis probe into the hippocampus, ACh levels in dialysates from the hippocampus increased markedly in dose and time dependent manner at 2-2.5 and 2.5-3 hr. AVP1-9, the parent peptide, has a similar enhancing effect on ACh release as AVP4-9. Stimulated ACh release by AVP4-9 was significantly inhibited by V1-selective receptor antagonist ([1-(beta-mercapto-beta,beta-cyclopentamethylenepropionic acid), 2-(O-methyl)-tyrosine]AVP), but not by V2-selective antagonist ([1-(beta-mercapto-beta,beta-cyclopentamethylenepropionic acid), 2-D-Ile, 4-Ile]AVP). From these observations, it is demonstrated that AVP4-9 stimulates the ACh release in rat hippocampus via mediating V1-like vasopressin receptors.
Maegawa
H
H
Preclinical Research Department, Ono Pharmaceutical Co., Ltd., Osaka, Japan.
Katsube
N
N
Okegawa
T
T
Aishita
H
H
Kawasaki
A
A
D000109:Acetylcholine / Q000378:metabolism*; D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D001127:Arginine Vasopressin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003956:Dialysis / Q000379:methods; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D008124:Locomotion; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D010446:Peptide Fragments; D001127:Arginine Vasopressin; C045881:argipressin (4-9); D000109:Acetylcholine