B-cell activation and differentiation by HIV-1 antigens among volunteers vaccinated with VaxSyn HIV-1.
The generation of memory B cells in response to vaccination with a baculovirus-derived recombinant gp160 candidate AIDS vaccine, VaxSyn HIV-1, was investigated in 12 healthy human volunteers who were immunized with VaxSyn HIV-1, hepatitis B vaccine, or alum adjuvant alone on days 1, 28, 180, and 540. Peripheral blood mononuclear cells were collected pre- and post-immunization and cultured unstimulated or with pokeweed mitogen (PWM), VaxSyn HIV-1 (rgp160), or HIV-1 lysate (iHIV-1) for 7 days before polyclonal and HIV-1-specific IgG production in culture supernatants (SNs) were measured. No differences were seen in the spontaneous or PWM-induced IgG production in SN from vaccinees and controls. Only vaccinee SN contained higher-than-normal levels of polyclonal IgG after stimulation with either rgp160 or iHIV-1, especially after the second and third booster immunizations on days 180 and 540, respectively. There were also contemporaneous increases in HIV-1-specific antibody in SN of all vaccinees, albeit at different time points throughout the study. We conclude that VaxSyn HIV-1 induces antigen-specific B-cell responses with the generation of memory B cells in vivo that can be reactivated in vitro to deliver an anamnestic response.
Reuben
J M
JM
Division of Laboratory Medicine, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Liang
L
L
Atmar
R L
RL
Greenberg
S
S
D016915:AIDS Vaccines / Q000008:administration & dosage*; D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D002458:Cell Fractionation; D002474:Cell-Free System; D003470:Culture Media; D005260:Female; D015483:HIV Antibodies / Q000096:biosynthesis; D015488:HIV Antigens / Q000502:physiology*; D015658:HIV Infections / Q000097:blood / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology
D016915:AIDS Vaccines; D003470:Culture Media; D015483:HIV Antibodies; D015488:HIV Antigens; D007074:Immunoglobulin G