Absence of autoantibodies to peptides shared by HLA-B27.5 and Klebsiella pneumoniae nitrogenase in serum samples from HLA-B27 positive patients with ankylosing spondylitis and Reiter's syndrome.
Some microorganisms which are pathogenic in humans share amino acid sequences with human proteins (molecular mimicry). It has been suggested that molecular mimicry might be a reason for autoimmunity as a result of immunological cross reactivity. A homologous sequence of six amino acids has been found in both Klebsiella pneumoniae nitrogenase and the HLA-B27.5 molecule. In addition, (auto)antibodies to a synthetic peptide that contained the HLA-B27.5/klebsiella mimicking epitope have been detected in serum samples from HLA-B27 positive patients with ankylosing spondylitis and Reiter's syndrome. Confirmation of these data is important, because ankylosing spondylitis and Reiter's syndrome have so far been assumed to be 'seronegative' rheumatic diseases. It was, however, not possible to confirm the presence of autoantibodies against the mimicking peptide in serum samples from patients with ankylosing spondylitis and Reiter's syndrome. Serum samples from 81 patients with ankylosing spondylitis, 38 patients with Reiter's syndrome, and 81 healthy blood donors were tested against the 'mimicking peptide' in an enzyme linked immunosorbent assay (ELISA). Some of the serum samples from patients showed high but non-specific binding to the mimicking peptide. A highly significant correlation between binding to plastic coated with the mimicking peptide, to plastic coated with an irrelevant peptide, and even to non-coated plastic was observed. The nature of the serum component(s) in these patient serum samples (and some control serum samples) responsible for the high non-specific binding to plastic remains unclear. It was also shown that antibodies to the HLA-B27 peptide (containing the mimicking epitope) induced in rabbits do not cross react with the klebsiella peptide and vice versa.
de Vries
D D
DD
Central Laboratory, The Netherlands, Red Cross Blood Transfusion Service, Amsterdam.
Dekker-Saeys
A J
AJ
Gyodi
E
E
Bohm
U
U
Ivanyi
P
P
D000328:Adult; D000368:Aged; D000818:Animals; D016918:Arthritis, Reactive / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis*; D003429:Cross Reactions; D005260:Female; D015796:HLA-B27 Antigen / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009591:Nitrogenase / Q000737:chemistry / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid; D013167:Spondylitis, Ankylosing / Q000276:immunology*
D001323:Autoantibodies; D015796:HLA-B27 Antigen; D010455:Peptides; D009591:Nitrogenase