Transcripts of the viroid central conserved region contain the local tertiary structural element found in full-length viroid.
The viroid central conserved region (CCR) is highly conserved among different viroids and is thought to be involved in viroid replication. A novel tertiary structure occurs in the CCR of native circular potato spindle tuber RNAs. To permit more detailed studies of this structural element, a small RNA oligonucleotide containing the CCR of the viroid genome was synthesized. The tertiary structure of these CCR transcripts was examined by UV-crosslinking of the RNA, followed by mapping of the crosslink using limited alkaline digestion and classical RNA secondary analysis. The CCR transcript was found to undergo UV-crosslinking between the same two bases as in full-length viroid, indicating that the tertiary structure is the same and that the CCR transcript will be useful for the affinity purification of host components.
Paul
C P
CP
Department of Biochemistry, Cornell University Medical College, New York, NY 10021.
Levine
B J
BJ
Robertson
H D
HD
Branch
A D
AD
D001483:Base Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays; D014772:Viroids / Q000235:genetics* / Q000528:radiation effects / Q000648:ultrastructure
D012367:RNA, Viral