Involvement of FAD-dependent polyamine oxidase in the metabolism of milacemide in the rat.
1. It has previously been established that monoamine oxidase (MAO)-B participates in the metabolism of milacemide [2-(pentylamino)acetamide]. Furthermore, in rats, inhibition of FAD-dependent polyamine oxidase (PAO) was found to decrease the urinary excretion of two milacemide metabolites, termed UK1 and UK2. 2. Using gas chromatography-mass spectrometry, UK1 was identified as oxamic acid and UK2 as 2-hydroxyacetamide, confirming that PAO is involved in the metabolism of milacemide. 3. Thus, two FAD-dependent amine-oxidizing enzymes, MAO and PAO, contribute to the metabolism of milacemide. Milacemide appears to be the first non-polyamine xenobiotic in the metabolism of which PAO participates.
Strolin Benedetti
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M
Farmitalia Carlo Erba, Research and Development-Erbamont Group, Milan, Italy.
Allievi
C
C
Cocchiara
G
G
Pevarello
P
P
Dostert
P
P
D000081:Acetamides / Q000652:urine*; D000818:Animals; D000927:Anticonvulsants / Q000652:urine*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D010072:Oxamic Acid / Q000652:urine; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000097667:Polyamine Oxidase
D000081:Acetamides; D000927:Anticonvulsants; D005182:Flavin-Adenine Dinucleotide; D010072:Oxamic Acid; D000587:Oxidoreductases Acting on CH-NH Group Donors; D000097667:Polyamine Oxidase; C039332:milacemide