A nonlethal intravenous dose of endotoxin influences opsonized zymosan-stimulated [32P]phospholipid turnover in rat alveolar macrophages.
The opsonized zymosan-stimulated turnover of 32P-labeled phospholipids was examined in alveolar macrophages from rats 3 hr after intravenous administration of saline or a nonlethal dose of endotoxin. Stimulation resulted in increased incorporation of [32P]PO4 into phosphatidic acid and phosphatidylinositol. Also a decreased [32P]phosphatidylcholine and an increased [32P]lysophosphatidylcholine labeling were observed, suggesting an increased activity of phosphatidylcholine-specific phospholipase A2. Endotoxin attenuated these changes in 32P-labeled phospholipids, demonstrating the ability of a nonlethal dose of endotoxin to perturb phospholipid-dependent signal transduction mechanisms in cells isolated from a compartment other than the one in which endotoxin was administered.
Dowty
B J
BJ
Department of Physiology, Louisiana State University Medical Center, New Orleans 70112.
Rodriguez de Turco
E
E
Hagar
A
A
Spitzer
J A
JA
D000818:Animals; D004731:Endotoxins / Q000494:pharmacology*; D007275:Injections, Intravenous; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D009895:Opsonin Proteins / Q000737:chemistry; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015398:Signal Transduction; D015054:Zymosan / Q000494:pharmacology*
D004731:Endotoxins; D009895:Opsonin Proteins; D010743:Phospholipids; D015054:Zymosan