title: Tissue renin-angiotensin systems in renal hypertension.
abstract: Angiotensinogen messenger RNA (mRNA) levels were measured in the brain (hypothalamus, lower brain stem, cerebellum), liver, kidneys, and adrenal glands of rats made hypertensive by ligation of the aorta between the renal arteries. We also measured renin mRNA in the kidneys of these renal hypertensive rats. The early phase of hypertension (day 6) was associated with significant increases in plasma renin activity and levels of circulating angiotensin II. The circulating renin-angiotensin system was not activated in the later phase of hypertension (day 24). Angiotensinogen mRNA levels were elevated in the lower brain stem of hypertensive rats at both stages of hypertension. In contrast, angiotensinogen mRNA levels in the hypothalamus were increased only at day 6 after aortic ligation. Decreased levels of angiotensinogen mRNA were observed in the cerebellum in both the early and later phases of the hypertension. Angiotensinogen mRNA levels in the adrenal gland below the ligature fell in the early phases but rose in the later phases of hypertension. Renin mRNA levels of the ischemic kidney remained elevated at both the early and later phases, whereas in both ischemic and nonischemic kidneys, levels of angiotensinogen mRNA remained below sham values throughout the period of study. These results indicate differential expression of renin-angiotensin system mRNAs in tissues of renal hypertensive rats. The differential changes in the expression of angiotensinogen mRNA over the course of development and maintenance of renal hypertension suggest that factors in addition to angiotensin II are important in modulating the expression of renin-angiotensin system genes.
authors.affiliation: Department of Brain and Vascular Research, Cleveland Clinic Foundation, Ohio 44195.
authors.forename: M
authors.initials: M
authors.lastname: Nishimura
authors.forename: A
authors.initials: A
authors.lastname: Milsted
authors.forename: C H
authors.initials: CH
authors.lastname: Block
authors.forename: K B
authors.initials: KB
authors.lastname: Brosnihan
authors.forename: C M
authors.initials: CM
authors.lastname: Ferrario
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism; D000808:Angiotensinogen / Q000235:genetics; D000818:Animals; D001011:Aorta; D001794:Blood Pressure; D001921:Brain / Q000378:metabolism; D006977:Hypertension, Renal / Q000378:metabolism* / Q000503:physiopathology; D007668:Kidney / Q000378:metabolism; D008026:Ligation; D008099:Liver / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000235:genetics; D012084:Renin-Angiotensin System
chemical_list: D012333:RNA, Messenger; D000808:Angiotensinogen; D012083:Renin