Purification of human leucocyte DNA: proteinase K is not necessary.
A rapid nontoxic method for the purification of DNA from human leucocytes is described. Preliminary experiments which tested different methods of DNA purification indicated that digestion of proteins with proteinase K was unnecessary. This led to the development of a simple procedure involving lysis of the cells in SDS followed by extraction with 6 M NaCl. The method described overcomes the requirement for lengthy incubations in the presence of expensive proteinase K and subsequent extraction with toxic chemicals.
Douglas
A M
AM
Victorian Institute of Forensic Pathology, South Melbourne, Australia.
Georgalis
A M
AM
Benton
L R
LR
Canavan
K L
KL
Atchison
B A
BA
D004247:DNA / Q000302:isolation & purification*; D019286:Endopeptidase K; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D012697:Serine Endopeptidases
D004247:DNA; D012697:Serine Endopeptidases; D019286:Endopeptidase K