Electrolytic lesions of the locus coeruleus or 6-hydroxydopamine lesions of the medial forebrain bundle protect against excitotoxic damage in rat hippocampus.
Unilateral lesioning of the rat locus coeruleus by an electric current (0.3 mA/10 s) reduces the ipsilateral hippocampal tissue content of dopamine (DA) and noradrenaline (NA) by 80% and 60% respectively, and protects against the excitotoxic action of bilateral kainate injections (1.1 nmol/microliters) in the hippocampus. On the ipsilateral side hippocampal CA1, CA2 and DG cells were protected; lesser protection of CA1 cells was seen on the contralateral side. The unilateral injection of 6-hydroxydopamine (8.8 micrograms/2 microliters/5 min) into the medial forebrain bundle abolishes the increase in extracellular NA concentration induced by kainate in the ipsilateral hippocampus without altering the low extracellular DA levels. This medial forebrain bundle lesion protects (on the ipsilateral side) against kainate toxicity (500 microM through the probe for 20 min) in the hippocampus (CA1, CA2 and DG cells). The noradrenergic innervation of the hippocampus apparently potentiates the excitotoxic effect of focal injection of kainate.
Arvin
B
B
Department of Neurology, Institute of Psychiatry, London, UK.
Le Peillet
E
E
Dürmüller
N
N
Chapman
A G
AG
Meldrum
B S
BS
D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism; D003956:Dialysis; D004558:Electric Stimulation; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D007608:Kainic Acid / Q000633:toxicity*; D008125:Locus Coeruleus / Q000033:anatomy & histology / Q000502:physiology*; D008297:Male; D008474:Medial Forebrain Bundle / Q000033:anatomy & histology / Q000502:physiology*; D009638:Norepinephrine / Q000502:physiology; D016627:Oxidopamine; D051381:Rats; D011919:Rats, Inbred Strains
D015306:Biogenic Monoamines; D016627:Oxidopamine; D007608:Kainic Acid; D009638:Norepinephrine