Role of interferon-gamma in experimental gram-negative sepsis.
To study the role of interferon-gamma (IFN-gamma) in gram-negative shock, mortality was compared in mice receiving either a monoclonal antibody to IFN-gamma (H22) or an irrelevant monoclonal antibody (L2-3D9) before or after an LD90 dose of Escherichia coli O111:B4. H22 given either 1 h before or 0.5 h after bacterial challenge protected mice from death (mortality at 48 h, 28% vs. 83%, P less than .001). Serum tumor necrosis factor-alpha (TNF alpha) levels and bacterial counts in blood and organs (liver, spleen, heart, and brain) were similar in H22-treated animals and controls. The peak serum TNF alpha levels were 95.7 +/- 16.4 ng/mL and 80.7 +/- 14.9 ng/mL in the H22 and control groups, respectively. These results indicate that IFN-gamma plays a significant role in the pathogenesis of gram-negative sepsis.
Silva
A T
AT
Department of Infectious Diseases and Bacteriology, Hammersmith Hospital, London, United Kingdom.
Cohen
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D016470:Bacteremia / Q000209:etiology* / Q000276:immunology / Q000628:therapy; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000254:growth & development / Q000276:immunology; D004927:Escherichia coli Infections / Q000209:etiology / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000209:etiology* / Q000276:immunology / Q000628:therapy; D007371:Interferon-gamma / Q000276:immunology / Q000502:physiology*; D008297:Male; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma