Appropriate in vivo expression of a muscle-specific promoter by using avian retroviral vectors for gene transfer [corrected].
The promoter regions of the chicken skeletal muscle alpha-actin (alpha sk-actin) and the cytoplasmic beta-actin genes were linked to the bacterial chloramphenicol acetyltransferase (CAT) gene. Replication-competent retroviral vectors were used to introduce these two actin/CAT cassettes into the chicken genome. Chickens infected with retroviruses containing the alpha sk-actin promoter expressed high levels of CAT activity in striated muscle (skeletal muscle and heart); much lower levels of CAT activity were produced in the other nonmuscle tissues. In contrast, chickens infected with retroviruses containing the beta-actin promoter linked to the CAT gene expressed low levels of CAT activity in many different tissue types and with no discernible tissue specificity. Data are presented to demonstrate that the high levels of CAT activity that were detected in the skeletal muscle of chickens infected with the retrovirus containing the alpha sk-actin promoter/CAT cassette were not due to preferential infectivity, integration, or replication of the retrovirus vector in the striated muscles of these animals.
Petropoulos
C J
CJ
ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201.
Payne
W
W
Salter
D W
DW
Hughes
S H
SH
D000199:Actins / Q000235:genetics; D000818:Animals; D001717:Birds / Q000382:microbiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D005347:Fibroblasts; D005822:Genetic Vectors / Q000235:genetics*; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics; D011533:Proviruses / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012190:Retroviridae / Q000235:genetics* / Q000254:growth & development; D014162:Transfection / Q000235:genetics*; D014779:Virus Replication
D000199:Actins; D004279:DNA, Viral; D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase