title: Cloning of a human choline kinase cDNA by complementation of the yeast cki mutation.
abstract: A human choline kinase cDNA was cloned by complementation of the yeast choline kinase mutation, cki, from a human glioblastoma cDNA expression library. The deduced sequence of the human enzyme comprised 456 amino acids with a calculated relative molecular mass of 52,065. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not much in the amino-terminal region.
authors.affiliation: Department of Biochemistry, Gunma University School of Medicine, Maebashi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hosaka
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: J
authors.initials: J
authors.lastname: Nikawa
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002797:Choline Kinase / Q000235:genetics*; D003001:Cloning, Molecular; D015723:Gene Library; D005816:Genetic Complementation Test; D006801:Humans; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D015003:Yeasts / Q000235:genetics
chemical_list: D002797:Choline Kinase