title: Immunochemical differences among Methanosarcina mazei S-6 morphologic forms.
abstract: Methanosarcinae are the only archaeobacteria known to undergo major morphologic changes during growth involving unicellular and multicellular forms, and Methanosarcina mazei S-6 is the only strain for which three distinct forms, packets, single cells, and lamina, have so far been observed. It is reported that two pairs of these forms, either packets and single cells or single cells and lamina, grew and interconverted in medium with the same composition, Ca2+ and Mg2+ concentrations, and growth substrate, and that the two forms in each pair displayed distinctive differences revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting, the same growth medium-substrate notwithstanding.
authors.affiliation: Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany.
authors.forename: R
authors.initials: R
authors.lastname: Yao
authors.forename: A J
authors.initials: AJ
authors.lastname: Macario
authors.forename: E
authors.initials: E
authors.lastname: Conway de Macario
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis*; D003208:Concanavalin A / Q000378:metabolism; D015151:Immunoblotting; D017020:Methanosarcina / Q000737:chemistry / Q000166:cytology* / Q000254:growth & development / Q000276:immunology; D009024:Morphogenesis
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D003208:Concanavalin A