title: NADP-dependent 15-hydroxyprostaglandin dehydrogenase is homologous to NAD-dependent 15-hydroxyprostaglandin dehydrogenase and other short-chain alcohol dehydrogenases.
abstract: Human tissues contain two enzymes that catalyze the oxidation of the 15-hydroxy group of prostaglandins: NAD-dependent 15-hydroxyprostaglandin dehydrogenase which is fairly specific for prostaglandins and NADP-dependent 15-hydroxyprostaglandin dehydrogenase with low specificity for prostaglandins. The recent determination of the amino acid sequence of the NAD-dependent enzyme has revealed similarities with a number of oxidoreductases with distinct specificities that constitute the short-chain alcohol dehydrogenase family. Comparison of the amino acid sequence of NADP-dependent 15-hydroxyprostaglandin dehydrogenase with the sequences of the NAD-dependent enzyme and other short-chain alcohol dehydrogenases revealed the same similarities, identifying the NADP-dependent enzyme as a short-chain alcohol dehydrogenase. The degree of homology between the two 15-hydroxyprostaglandin dehydrogenases is similar to the one between each of the two enzymes and other members of the family indicating an early evolutionary divergence of the two proteins.
authors.affiliation: University of Berne, Department of Clinical Chemistry, Inselspital, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Wermuth
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D009249:NADP / Q000494:pharmacology*; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity
chemical_list: D009249:NADP; D006911:Hydroxyprostaglandin Dehydrogenases; D000426:Alcohol Dehydrogenase; C035859:15-hydroxyprostaglandin dehydrogenase