title: Detection of antibodies to human immunodeficiency virus type 2 (HIV-2) in blood donor sera using United States assay methods for anti-HIV type 1.
abstract: Twelve serum samples from French blood donors that were uniformly reactive in tests for antibody to human immunodeficiency virus type 2 (anti-HIV-2) also were reactive in 92 to 100 percent of tests with three anti-HIV type 1 (anti-HIV-1) enzyme-linked immunoassays currently in widespread use for donor screening in the United States. Supplemental tests for anti-HIV-1 on these anti-HIV-2-reactive samples differed in their responses. All samples reacted in a licensed anti-HIV-1 Western blot, but there was an atypical band near the p41 position, which could be a clue to the fact that this result was a cross-reaction with anti-HIV-2. A recombinant immunoblot gave an indeterminate result for anti-HIV-1 in all 12 samples. A local immunofluorescence assay for anti-HIV-1 reacted with 92 percent of the samples, but a commercial one detected only 58 percent.
authors.affiliation: Sacramento Medical Foundation Center for Blood Research, California.
authors.forename: K
authors.initials: K
authors.lastname: Sazama
authors.forename: I K
authors.initials: IK
authors.lastname: Kuramoto
authors.forename: P V
authors.initials: PV
authors.lastname: Holland
authors.forename: A M
authors.initials: AM
authors.lastname: Couroucé
authors.forename: D
authors.initials: D
authors.lastname: Gallo
authors.forename: C V
authors.initials: CV
authors.lastname: Hanson
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D001782:Blood Donors; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D008722:Methods
chemical_list: D000914:Antibodies, Viral