title: A radiation-reduced hybrid cell line containing 5 Mb/17 cM of human DNA from 9q34.
abstract: Disease gene loci for tuberous sclerosis (TSC1), idiopathic torsion dystonia (DYT1), and nail-patella syndrome (NPS1) have been mapped by genetic linkage analysis to human chromosome 9q band 34. To create a resource for physical mapping and manipulation of this region of the genome, we have created a radiation-reduced hybrid cell line containing DNA from human 9q34 as its only human component. This cell line, E6B, has been characterized by Southern blot and PCR analysis using a panel of 9q markers and fluorescent in situ hybridization. We estimate that it contains 5 Mb of human DNA, equal to 17 cM of genetic distance, extending from AK1 to ABO on 9q34.
authors.affiliation: Division of Experimental Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115.
authors.forename: E P
authors.initials: EP
authors.lastname: Henske
authors.forename: L
authors.initials: L
authors.lastname: Ozelius
authors.forename: M A
authors.initials: MA
authors.lastname: Anderson
authors.forename: D J
authors.initials: DJ
authors.lastname: Kwiatkowski
mesh_terms: D002460:Cell Line; D002874:Chromosome Mapping / Q000379:methods; D002899:Chromosomes, Human, Pair 9; D004247:DNA / Q000235:genetics*; D004422:Dystonia Musculorum Deformans / Q000235:genetics; D005453:Fluorescence; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D009261:Nail-Patella Syndrome / Q000235:genetics; D009693:Nucleic Acid Hybridization; D014402:Tuberous Sclerosis / Q000235:genetics
chemical_list: D004247:DNA