title: Automated determination of disopyramide and N-monodealkyldisopyramide in plasma by reversed-phase liquid chromatography with a column switching system.
abstract: A rapid and simple column liquid chromatographic method involving a column switching system for the determination of disopyramide and its N-monodealkyl metabolite (NMD) in plasma is described. The deproteinized plasma is applied to an automated system. Purification and concentration were performed using a precolumn connected to a six-position valve; analytical separation was done on-line using a cyano reversed-phase column with a mobile phase consisting of 10 mmol/l trimethylamine (pH 2.5, adjusted with phosphoric acid)-acetonitrile-tetrahydrofuran (78:20:2, v/v/v). Absorbance was measured at 265 nm, with a minimum detectable amount of disopyramide and NMD of 0.1 micrograms/ml. The method can be applied to drug monitoring and pharmacokinetic studies.
authors.affiliation: Département de Pharmacologie Clinique, CHRU, Nancy, France.
authors.forename: A
authors.initials: A
authors.lastname: Del Cont Bernard
authors.forename: M J
authors.initials: MJ
authors.lastname: Royer Morrot
authors.forename: A
authors.initials: A
authors.lastname: Zhiri
authors.forename: M
authors.initials: M
authors.lastname: Rambourg
authors.forename: R J
authors.initials: RJ
authors.lastname: Royer
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D004206:Disopyramide / Q000031:analogs & derivatives* / Q000097:blood*; D010276:Parasympatholytics / Q000097:blood*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010276:Parasympatholytics; C030438:4-(isopropylamino)-2-(2-pyridyl)-2-phenylbutyramide; D004206:Disopyramide