Incomplete testicular feminization syndrome: studies of 17 beta-oestradiol-binding activity and aromatase activity in cultured genital fibroblasts showing impaired dihydrotestosterone-binding.
Dihydrotestosterone (DHT) and 17 beta-oestradiol binding, and aromatase activity were measured in cultured genital skin fibroblasts from two siblings with the incomplete testicular feminization syndrome. Whole-cell and nuclear DHT binding in the cells from both patients were reduced to 80% and 60%, respectively of those in a normal male subject. The nuclear oestradiol binding was not impaired in the fibroblasts from the patients whose plasma oestradiol was normal or a little elevated. Although gonadectomy led to a decrease in plasma testosterone concentration, the concentration of testosterone-binding globulin showed no change suggesting no apparent effect on testosterone-binding globulin synthesis by testicular steroids. There was no significant difference in aromatase activity of the fibroblasts between the patients and the normal male subjects. The relatively increased oestrogen concentration in the syndrome might result from oestrogen production in testes, but is unlikely to be from peripheral tissues such as fibroblasts. This is the first report to examine oestradiol binding and aromatase activity in the cells from the incomplete testicular feminization associated with impaired DHT binding. These findings may give new insight into the pathogenesis of abnormal male sexual differentiation in the patients with testicular feminization syndrome.
Imai
A
A
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
Ohno
T
T
Nakagawa
M
M
Sawairi
M
M
Tamaya
T
T
D000328:Adult; D013734:Androgen-Insensitivity Syndrome / Q000378:metabolism*; D001141:Aromatase / Q000378:metabolism*; D002478:Cells, Cultured; D013196:Dihydrotestosterone / Q000378:metabolism*; D004958:Estradiol / Q000032:analysis / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005837:Genitalia, Male / Q000378:metabolism*; D006801:Humans; D008297:Male
D013196:Dihydrotestosterone; D004958:Estradiol; D001141:Aromatase