Effect of endotoxin on cultured chinchilla middle ear epithelium.
The effect of endotoxin on the growth and morphology of cultured chinchilla middle ear epithelium was examined. Within 24 hours of culture, cells maintained in medium containing endotoxin exhibited dose-dependent morphological changes. The highest concentration of endotoxin (100 mg) resulted in cell death. The growth curve of the cells in culture demonstrated a dose-dependent, significant increase in cell number when endotoxin was added to the medium.
Nakamura
A
A
Department of Otolaryngology, Ohio State University College of Medicine, Columbus.
DeMaria
T F
TF
van Blitterswijk
C
C
Lim
D J
DJ
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002682:Chinchilla; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000502:physiology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D012486:Salmonella typhimurium; D014432:Tympanic Membrane / Q000166:cytology / Q000187:drug effects*
D004731:Endotoxins