Selective toxicity of ethacrynic acid towards lymphocytes of chronic lymphocytic leukemia in vitro.
We have used the MTT colorimetric assay to determine the sensitivity to ethacrynic acid of lymphocytes from normal donors and of peripheral blood cells from leukaemia patients. Whereas normal lymphocytes and cells from acute or chronic myeloid leukaemia showed similar sensitivities (median inhibitory dose, ID50 = 20-22 microM), lymphocytes from chronic lymphocytic leukaemia patients were much more sensitive (ID50 = 6 microM). This result was found irrespective of the assay duration.
Twentyman
P R
PR
Department of Clinical Oncology and Radiotherapeutics, University of Cambridge, UK.
Lambert
E
E
Müller
M
M
Rees
J K
JK
D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004976:Ethacrynic Acid / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000188:drug therapy*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy; D008214:Lymphocytes / Q000187:drug effects*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004976:Ethacrynic Acid