[Bacillus subtilis gene rec223: molecular cloning and proposed function of its protein product].
Chromosomal DNA fragment which complemented rec223 mutation of Bacillus subtilis was cloned. Introduction of one copy of the cloned gene into the cells of the rec mutant restored both normal activity for DNA damages repair after mitomycin C action and recombination proficiency. Using multicopy vector led to no formation of recombinants, which was probably connected with overproduction of rec223 gene protein product in Bacillus subtilis cells.
Gavrilova
E V
EV
Mekhedov
S L
SL
Prozorov
A A
AA
Khasanov
F K
FK
D001412:Bacillus subtilis / Q000235:genetics*; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016685:Mitomycin / Q000494:pharmacology; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D016685:Mitomycin
Gen rec223 Bacillus subtilis: molekuliarnoe klonirovanie i predpolagaemye funktsii ego belkovogo produkta.