title: Prosomes and their multicatalytic proteinase activity.
abstract: Prosomes were first described as being mRNA-associated RNP (ribonucleoprotein) particles and subcomponents of repressed mRNPs (messenger ribonucleoprotein). We show here that prosomes isolated from translationally inactive mRNP have a protease activity identical to that described by others for the multicatalytic proteinase complex (MCP, 'proteasome'). By RNase or non-ionic detergent treatment, the MCP activity associated with repressed non-globin mRNP from avian erythroblasts, sedimenting at 35 S, could be quantitatively shifted on sucrose gradients to the 19-S sedimentation zone characteristic of prosomes, which were identified by monoclonal antibodies. The presence of small RNA in the enzymatic complex was shown by immunoprecipitation of the protease activity out of dissociated mRNP using a mixture of anti-prosome monoclonal antibodies; a set of small RNAs 80-120 nucleotides long was isolated from the immunoprecipitate. Furthermore, on CsCl gradients, colocalisation of the MCP activity with prosomal proteins and prosomal RNA was found, and no difference in the prosomal RNA pattern was observed whether the particles were fixed or not prior to centrifugation. These data indicate that the MCP activity is a property of prosomes, shown to be in part RNP and subcomplexes of in vivo untranslated mRNP. A hypothesis for the role of the prosome-MCP particles in maintaining homeostasis of specific protein levels is proposed.
authors.affiliation: Institut Jacques Monod, CNRS, Université Paris 7, France.
authors.forename: H G
authors.initials: HG
authors.lastname: Nothwang
authors.forename: O
authors.initials: O
authors.lastname: Coux
authors.forename: F
authors.initials: F
authors.lastname: Bey
authors.forename: K
authors.initials: K
authors.lastname: Scherrer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004372:Ducks; D004900:Erythroblasts / Q000737:chemistry*; D005786:Gene Expression Regulation; D006367:HeLa Cells / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism*; D011233:Precipitin Tests; D046988:Proteasome Endopeptidase Complex; D014176:Protein Biosynthesis; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
chemical_list: D009097:Multienzyme Complexes; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex