title: Sensitive stereospecific assay of warfarin in plasma: reversed-phase high-performance liquid chromatographic separation using diastereoisomeric esters of (-)-(1S,2R,4R)-endo-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]-hept-5- ene-2-carboxylic acid.
abstract: A stereospecific reversed-phase high-performance liquid chromatographic (HPLC) assay for warfarin in plasma has been developed. The assay involves a rapid, simple clean-up procedure which separates warfarin from plasma constituents and warfarin metabolites. Warfarin enantiomers were assayed as their (-)-(1S,2R,4R)-endo-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]hept-5-ene -2- carboxylic acid diastereoisomeric esters by reversed-phase HPLC. Excellent resolution of the diastereoisomers was achieved in less than 10 min. Sensitivity of the assay was approximately 5-10 ng/ml for each isomer.
authors.affiliation: Department of Pharmacy, University of Sydney, N.S.W., Australia.
authors.forename: S R
authors.initials: SR
authors.lastname: Carter
authors.forename: C C
authors.initials: CC
authors.lastname: Duke
authors.forename: D J
authors.initials: DJ
authors.lastname: Cutler
authors.forename: G M
authors.initials: GM
authors.lastname: Holder
mesh_terms: D001643:Bridged Bicyclo Compounds / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D015203:Reproducibility of Results; D013237:Stereoisomerism; D014859:Warfarin / Q000097:blood* / Q000737:chemistry*
chemical_list: D001643:Bridged Bicyclo Compounds; C058455:1,4,5,6,7,7-hexachlorobicyclo(2.2.1)hept-5-ene-2-carboxylic acid; D014859:Warfarin