title: A single mode fibre-optic evanescent wave biosensor.
abstract: This paper reports experimental developments in the construction and operation of a single-mode fibre-optic evanescent wave biosensor using an exposed core silica single-mode fibre embedded in a silica block. The device was able to monitor the concentration of a blue dye, Procion Blue MX-G, in overlayers of various refractive indices. The practicality of such a biosensor has been demonstrated with a colorimetric enzyme assay system. Penicillin G in the 0-0.4 mM concentration range was monitored at 633 nm by the decoloration of the starch-iodine reagent when Bacillus cereus penicillinase was immobilized over the exposed core of the monomode fibre.
authors.affiliation: Institute of Biotechnology, University of Cambridge, UK.
authors.forename: E E
authors.initials: EE
authors.lastname: Carlyon
authors.forename: C R
authors.initials: CR
authors.lastname: Lowe
authors.forename: D
authors.initials: D
authors.lastname: Reid
authors.forename: I
authors.initials: I
authors.lastname: Bennion
mesh_terms: D015374:Biosensing Techniques; D003124:Colorimetry; D004396:Coloring Agents; D005336:Fiber Optic Technology; D010400:Penicillin G / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods
chemical_list: D004396:Coloring Agents; D010400:Penicillin G