Identification of cholesterol-bound aldehydes in copper-oxidized low density lipoprotein.
Lipid-soluble cholesteryl ester core aldehydes (aldehydes still bound to the cholesterol ring) were identified among the products of copper-catalyzed peroxidation of human low density lipoprotein (LDL). The LDL was exposed to oxygenated buffer and 5 microM CuSO4 for 24 h. The core aldehydes were isolated as the dinitrophenylhydrazones, and were identified by reverse-phase HPLC with mass spectrometry. The major components were the C4-C10 oxoalkanoyl esters of cholesterol and 7-ketocholesterol, and accounted for 1-2% of the cholesteryl linoleate and arachidonate consumed.
Kamido
H
H
Banting and Best Department of Medical Research, University of Toronto, Canada.
Kuksis
A
A
Marai
L
L
Myher
J J
JJ
D000447:Aldehydes / Q000032:analysis*; D002784:Cholesterol / Q000031:analogs & derivatives*; D003300:Copper / Q000737:chemistry; D006801:Humans; D006835:Hydrazones / Q000032:analysis* / Q000737:chemistry; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000737:chemistry*
C001072:2,4-dinitrophenylhydrazone; D000447:Aldehydes; D006835:Hydrazones; D008077:Lipoproteins, LDL; D003300:Copper; D002784:Cholesterol