title: Cloning of the gamma chain of the human IL-2 receptor.
abstract: A third subunit, the gamma chain, of the human interleukin-2 receptor (IL-2R) was identified, and a complementary DNA clone encoding this member of the cytokine receptor family was isolated. The gamma chain is necessary for the formation of the high- and intermediate-affinity receptors, which consists of alpha beta gamma heterotrimers and beta gamma heterodimers, respectively. The IL-2R on murine fibroblastoid cells can be internalized after binding IL-2 only if the gamma chain is present; alpha and beta are insufficient for internalization. Thus, the gamma chain is an indispensable component of the functional IL-2R.
authors.affiliation: Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takeshita
authors.forename: H
authors.initials: H
authors.lastname: Asao
authors.forename: K
authors.initials: K
authors.lastname: Ohtani
authors.forename: N
authors.initials: N
authors.lastname: Ishii
authors.forename: S
authors.initials: S
authors.lastname: Kumaki
authors.forename: N
authors.initials: N
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Munakata
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Sugamura
mesh_terms: D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D007376:Interleukin-2; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2