title: Cloning and primary structure of the wide-spectrum amidase from Brevibacterium sp. R312: high homology to the amiE product from Pseudomonas aeruginosa.
abstract: A Brevibacterium sp. R312 DNA fragment encoding the wide-spectrum amidase (EC 3.5.1.4) has been cloned and sequenced, using limited amino acid (aa) sequence information obtained from the purified enzyme. The deduced aa sequence showed more than 80% strict identity with the Pseudomonas aeruginosa aliphatic amidase, the product of the amiE gene, suggesting a horizontal transfer of the gene during evolution between Gram+ and Gram- bacteria.
authors.affiliation: Département Biotechnologie, Rhône-Poulenc Rorer, Centre de Recherche de Vitry-Alfortville, Vitry sur Seine, France.
authors.forename: F
authors.initials: F
authors.lastname: Soubrier
authors.forename: S
authors.initials: S
authors.lastname: Lévy-Schil
authors.forename: J F
authors.initials: JF
authors.lastname: Mayaux
authors.forename: D
authors.initials: D
authors.lastname: Pétré
authors.forename: A
authors.initials: A
authors.lastname: Arnaud
authors.forename: J
authors.initials: J
authors.lastname: Crouzet
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D001951:Brevibacterium / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping
chemical_list: D011994:Recombinant Proteins; D000581:Amidohydrolases; C019665:amidase