[A simple method of isolating monoclonal immunoglobulin M, possessing rheumatoid activity].
A simple procedure for obtaining highly purified preparations of native monoclonal (Waldenström's disease) immunoglobulin M possessing a rheumatoid activity (IgM-RF) has been developed. The method is based on the use of affinity chromatography with a new readily available adsorbent (immunoglobulin G-porous glass) and 3 M LiCl in Tris-buffer pH 8.3-8.4 able to induce the dissociation of the IgM-RF-IgG complex. The IgM-RF preparation thus obtained was characterized in terms of amino acid composition (relative to conventional monoclonal IgM), carbohydrate composition and structure of oligosaccharide moieties of a complex type. It was shown that some dissociation conditions for the IgM-RF-IgG complex routinely used to isolate IgM-RF provoke irreversible denaturation of IgM-RF when applied to a preliminarily purified complex.
Lapuk
V A
VA
Chukhrova
A I
AI
Chernokhvostova
E V
EV
Aleshkin
V A
VA
German
G P
GP
Varlamova
E Iu
EIu
Ponomareva
A M
AM
Arbatskiĭ
N P
NP
Zheltova
A O
AO
D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000276:immunology*; D008969:Molecular Sequence Data; D011489:Protein Denaturation; D012217:Rheumatoid Factor / Q000276:immunology*
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D007075:Immunoglobulin M; D012217:Rheumatoid Factor
Prostoĭ metod vydeleniia monoklonal'nogo immunobloulina M, obladaiushchego revmatoidnoĭ aktivnost'iu.