Estimation and separation of N-acetyl-beta-D-glucosaminidase isoenzymes in urine.
N-acetyl-beta-D-glucosaminidase is a lysosomal enzyme made up of two isoenzymes (A and B). It has been used extensively as a marker for kidney damage. However, its estimation in urine has not been standardized. We have established a method for the estimation and separation of NAG isoenzymes by ion-exchange chromatography. In 19 experiments done so far, this method has given reproducible results. The significance of this method is that with a single experiment, one can estimate total as well as individual isoenzyme activity. Furthermore, urine constituents do not appear to interfere in this assay.
Javed
M H
MH
Department of Biochemistry, Aga Khan University Medical College, Karachi.
Hussain
A N
AN
Waqar
M A
MA
D000118:Acetylglucosaminidase / Q000652:urine*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D007527:Isoenzymes / Q000652:urine*; D007669:Kidney Calculi / Q000652:urine*
D007527:Isoenzymes; D000118:Acetylglucosaminidase