title: Ferrioxamine uptake in Yersinia enterocolitica: characterization of the receptor protein FoxA.
abstract: The gene for the high-affinity outer membrane ferrioxamine receptor FoxA of Yersinia enterocolitica was cloned in Escherichia coli K-12. A foxA mutant of Yersinia could be complemented by the cloned DNA fragment. The FoxA encoding region was sequenced and an open reading frame encoding 710 amino acids, including a signal sequence of 26 amino acids, was deduced. The mature FoxA protein consisted of 684 amino acids and had a molecular mass of 75,768 Da. FoxA shared 33% amino acid sequence homology with FhuA, the ferrichrome receptor of Escherichia coli. Based on the homologies with FhuA and other TonB-dependent receptors a topological model of FoxA is presented.
authors.affiliation: Mikrobiologie II, Universität Tübingen, Germany.
authors.forename: A J
authors.initials: AJ
authors.lastname: Bäumler
authors.forename: K
authors.initials: K
authors.lastname: Hantke
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D016384:Consensus Sequence; D003676:Deferoxamine / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005290:Ferric Compounds / Q000378:metabolism*; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D011487:Protein Conformation; D021382:Protein Sorting Signals / Q000235:genetics; D011956:Receptors, Cell Surface; D011955:Receptors, Drug / Q000235:genetics* / Q000378:metabolism; D011991:Receptors, Virus / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D016354:Rhodobacter capsulatus / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D015008:Yersinia enterocolitica / Q000235:genetics / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D005290:Ferric Compounds; C050900:FhuA protein, E coli; C489524:FoxA protein, bacteria; C489523:FoxB protein, E coli; D008565:Membrane Proteins; D021382:Protein Sorting Signals; D011956:Receptors, Cell Surface; D011955:Receptors, Drug; D011991:Receptors, Virus; D011994:Recombinant Proteins; C057616:tonB protein, Bacteria; C489181:tonB protein, E coli; C002577:ferrioxamine B; D003676:Deferoxamine