title: Thyroid hormone, L-T3, stimulates the expression of the angiotensinogen gene in cultured opossum kidney (OK) cells.
abstract: Angiotensinogen (ANG) messenger RNA is expressed in opossum kidney (OK) proximal tubular cells. To examine whether thyroid hormone, L-T3, could stimulate the expression of the ANG gene in OK proximal tubular cells, fusion genes, consisting of various lengths of the 5'-flanking region of the rat angiotensinogen gene linked to a human growth hormone reporter gene, were constructed and introduced into OK cells. As a negative control, they were introduced into a nonkidney cell line, a human choriocarcinoma cell line (JEG-3). The level of the expression of fusion genes in these cells were determined by the level of immunoreactive human growth hormone secreted into the culture medium. The expression of ANG-growth hormone (ANG-GH) fusion genes pOGH (ANG N-1498/+18), pOGH (ANG N-688/+18), pOGH (ANG N-110/+18), pOGH (ANG N-53/+18), and pOGH (ANG N-35/+18) was 226-, 4.5-, 1.0-, 12-, and 2.5-fold higher than promoterless pOGH in the expression of growth hormone activity in OK cells. No significant expression of any of these ANG-GH fusion genes over the promoterless pOGH was observed in JEG-3 cells. The addition of L-T3 stimulates the expression of pOGH (ANG N-1498/+18) in a dose-dependent manner with a maximal and half-maximal effect at 10(-7) M and at 10(-8) to 10(-9) M, respectively. Thyroid hormone (10(-7) M) also stimulates the expression of pOGH (ANG N-688/+18) but not pOGH (ANG N-110/+18), pOGH (ANG N-53/+18), or pOGH (ANG N-35/+18).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: University of Montreal, Maisonneuve-Rosemont Hospital, Research Center, Quebec, Canada.
authors.forename: J S
authors.initials: JS
authors.lastname: Chan
authors.forename: A H
authors.initials: AH
authors.lastname: Chan
authors.forename: Z R
authors.initials: ZR
authors.lastname: Nie
authors.forename: R
authors.initials: R
authors.lastname: Sikstrom
authors.forename: S
authors.initials: S
authors.lastname: Lachance
authors.forename: S
authors.initials: S
authors.lastname: Hashimoto
authors.forename: S
authors.initials: S
authors.lastname: Carrière
mesh_terms: D000808:Angiotensinogen / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism*; D009893:Opossums; D012333:RNA, Messenger / Q000378:metabolism*; D014284:Triiodothyronine / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D014284:Triiodothyronine; D000808:Angiotensinogen