Assay of erythromycin in tape strips of human stratum corneum and some preliminary results in man.
The antibiotic erythromycin is used topically in the treatment of acne vulgaris. In order to evaluate the in vivo cutaneous absorption behavior of erythromycin in man, an analytical procedure for erythromycin in stratum corneum tape strips was developed. Erythromycin was extracted from tape strips using solid-phase extraction, followed by alkaline diethyl ether extraction. Reconstituted extracts were analyzed by RP-HPLC with electrochemical detection. This procedure proved to be adequate in a pilot experiment in man, topical erythromycin appearing to be distributed over the stratum corneum, its upper layers displaying an inward decreasing gradient.
van Hoogdalem
E J
EJ
Department of Biopharmaceutical Research, Brocades Pharma B.V., Delft, The Netherlands.
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004917:Erythromycin / Q000032:analysis*; D006801:Humans; D012867:Skin / Q000737:chemistry*
D004917:Erythromycin