High-performance liquid chromatographic method with fluorescence detection for the determination of total homocyst(e)ine in plasma.
A high-performance liquid chromatographic method for the determination of total plasma homocyst(e)ine [H(e)] after reduction with sodium tetrahydroborate and precolumn derivatization with o-phthaldialdehyde is described. The analyses, carried out on a reversed-phase C18 column, were based on spectrofluorimetric detection. The sensitivity was 1 pmol per injection and the intra- and inter-assay relative standard deviations were 1.8% and 5%, respectively. The plasma H(e) concentration determined in 40 healthy volunteers (20-60 years old) was 12.4 +/- 2.9 microM (mean +/- S.D.), in good agreement with reference values.
Fermo
I
I
Istituto Scientifico, Hosp. San Raffaele, Milan, Italy.
Arcelloni
C
C
De Vecchi
E
E
Viganò
S
S
Paroni
R
R
D000328:Adult; D001894:Borohydrides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008875:Middle Aged; D010084:Oxidation-Reduction; D012016:Reference Values; D013050:Spectrometry, Fluorescence
D001894:Borohydrides; D006710:Homocysteine; C025364:sodium borohydride