Tumorigenic effects mediated in the avian embryo by one or more oncogenes associated with v-myc.
We have previously shown that introduction of the v-myc oncogene in chick or quail embryos at E3 induces rapidly growing heart rhabdomyomas. We now report that a retrovirus containing one or two other oncogenes induces additional pathologies specified by the v-myc-associated oncogene. The v-mil/myc combination introduced at E3 induces, in addition to heart rhabdomyomas, tumors of proliferating cells aggregated onto the luminal aspect of vessels in both chick and quail embryos. In the quail these cells react positively with the quail-specific mAb QH1, which recognizes endothelial and most hemopoietic cells, while chick intravascular cells do not react with the chick-specific mAb VIA2 that recognizes hemopoietic cells. Thus the v-mil/myc tumors appear to be of endothelial origin. The v-myb-ets/myc combination injected at E3 induces cardiorhabdomyomas and aggressive VIA2-positive hemopoietic tumors in chick embryos, but only the v-myc-induced cardiorhabdomyomas in quail embryos. When injected into hatched animals, v-myc alone transforms hemopoietic and perhaps endothelial cells, but not cardiac cells. Thus the developmental stage at which a cell type can be transformed by v-myc and another associated oncogene depends on as yet undefined species-specific factors. More importantly, several examples of oncogene cooperation in vivo are adduced by these experiments. The type of cell transformed is specified by the viral oncogene combination.
al Moustafa
A E
AE
Institut d'Embryologie cellulaire et moléculaire du CNRS, Nogent Marne, France.
Quatannens
B
B
Dieterlen-Lièvre
F
F
Saule
S
S
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002642:Chick Embryo; D004625:Embryo, Nonmammalian; D005455:Fluorescent Antibody Technique; D016259:Genes, myc / Q000235:genetics*; D006338:Heart Neoplasms / Q000235:genetics*; D027384:Oncogene Proteins v-raf; D009857:Oncogenes / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011784:Quail; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics; D012207:Rhabdomyoma / Q000235:genetics*; D012878:Skin Neoplasms / Q000235:genetics*
D015682:Retroviridae Proteins, Oncogenic; C050379:oncogene proteins v-ets; D011505:Protein-Tyrosine Kinases; D027384:Oncogene Proteins v-raf