title: Interleukin-5 modulates eosinophil accumulation in allergic guinea pig lung.
abstract: Based on its involvement in eosinophil biology, interleukin 5 (IL-5) may play a role in the pulmonary eosinophilia associated with allergic reactions. We have examined that hypothesis using a neutralizing antibody to IL-5 in ovalbumin-sensitized guinea pigs challenged with aerosolized antigen. The extent of eosinophilia has been quantitated in bronchoalveolar lavage (BAL) and by histologic evaluation of lung tissue sections. Acute intraperitoneal administration of a rat IgG, monoclonal antibody to murine IL-5 derived from TRFK-5 cells prevented lung and BAL eosinophilia in a dose-dependent fashion at and above 10 micrograms per guinea pig. Treatment with either an experimentally irrelevant, isotype-matched antibody from GL113 cells or with heat-denatured IL-5 antibody was without effect. These studies demonstrate the importance of IL-5 to pulmonary eosinophilia in challenged, allergic guinea pigs.
authors.affiliation: Schering-Plough Research, Allergy and Cell Biology, Bloomfield, New Jersey 07003.
authors.forename: A R
authors.initials: AR
authors.lastname: Gulbenkian
authors.forename: R W
authors.initials: RW
authors.lastname: Egan
authors.forename: X
authors.initials: X
authors.lastname: Fernandez
authors.forename: H
authors.initials: H
authors.lastname: Jones
authors.forename: W
authors.initials: W
authors.lastname: Kreutner
authors.forename: T
authors.initials: T
authors.lastname: Kung
authors.forename: F
authors.initials: F
authors.lastname: Payvandi
authors.forename: L
authors.initials: L
authors.lastname: Sullivan
authors.forename: J A
authors.initials: JA
authors.lastname: Zurcher
authors.forename: A S
authors.initials: AS
authors.lastname: Watnick
mesh_terms: D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D001992:Bronchoalveolar Lavage Fluid / Q000473:pathology; D004804:Eosinophils / Q000473:pathology*; D006168:Guinea Pigs; D007114:Immunization; D015848:Interleukin-5 / Q000276:immunology / Q000494:pharmacology*; D008168:Lung / Q000473:pathology*; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000473:pathology*
chemical_list: D000906:Antibodies; D015848:Interleukin-5; D010047:Ovalbumin