Interferon gamma alters the phenotype of rat thymic epithelial cells in culture and increases interleukin-6 production.
Rat thymic epithelial cells (TEC) in long-term culture were characterized by anticytokeratin monoclonal antibodies (mAbs) and electron microscopy. Phenotypic analysis performed by a large panel of mAbs showed that the highest percentage of these cells was of the subcapsular/medullary type. Recombinant rat interferon (IFN)-gamma up-regulated class-I and class-II MHC expression by TEC in culture as confirmed by immunohistochemistry and flow cytometry, but did not significantly alter other cell markers. TEC supernatants of IFN-gamma-treated cultures showed higher interleukin-6 (IL-6) activity, compared to the control, as determined by proliferation of the IL-6-sensitive B9-cell line. Increased IL-6 activity was probably not a consequence of increased TEC number in IFN-gamma-treated cultures because IFN did not significantly stimulate TEC proliferation in vitro. In contrast, IL-6 significantly stimulated TEC proliferation, indicating that this cytokine is not only a regulatory molecule for T-cell proliferation, but could also be an autocrine growth factor for thymic epithelium.
Colić
M
M
Institute of Medical Research, Military Medical Academy, Belgrade, Yugoslavia.
Pejnović
N
N
Kataranovski
M
M
Popović
L
L
Gassić
S
S
Dujić
A
A
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D003470:Culture Media; D004848:Epithelium / Q000187:drug effects; D005260:Female; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology*
D003470:Culture Media; D015850:Interleukin-6; D011994:Recombinant Proteins; D007371:Interferon-gamma