An in vivo 19F NMR study of isoflurane elimination as a function of age in rat brain.
In vivo 19F NMR at 4.7 T has shown that the biphasic elimination of the vapor anesthetic isoflurane from rat brain is ca 15% slower in old (23-24 months) animals compared with young (5-6 months) animals. The fast kinetic component has a t1/2 of ca 7-9 min and the slow event, 100-115 min. Gas chromatographic measurement of arterial blood elimination displays age attenuation to the same extent, although a monophasic kinetic process (6-7 min). The slow wash-out from brain is thought to involve elimination from intracranial fatty tissue as postulated by others in rabbit brain. Longitudinal relaxation time measurements show monoexponential recovery and essentially identical values for young (1.09 + 0.11 s) and old (1.04 +/- 0.09 s) animals. For dipalmitoylphosphatidylcholine vesicles the monoexponential recovery also suggests rapidly exchanging averaged homogeneous lipid environments for the anesthetic, but the longer T1s (2.75 +/- 0.25 s) imply less restricted mobility compared with brain. Single T2 values were obtained in vivo, indicating either a single compartment or rapid exchange between multiple environments. These measurements were inconsistent, undoubtedly as a result of B1 inhomogeneity. The age-attenuated elimination kinetics for isoflurane are consistent with poorer cardiopulmonary function, whereas the T1 data suggest similar environments for the anesthetic in young and old brain tissue.
Chen
M
M
Department of Medicinal Chemistry, University of Utah, Salt Lake City 84112.
Olsen
J I
JI
Stolk
J A
JA
Schweizer
M P
MP
Sha
M
M
Ueda
I
I
D015060:1,2-Dipalmitoylphosphatidylcholine; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002849:Chromatography, Gas; D005461:Fluorine; D007530:Isoflurane / Q000097:blood / Q000493:pharmacokinetics*; D007554:Isotopes; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344 / Q000378:metabolism
D007554:Isotopes; D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D005461:Fluorine; D007530:Isoflurane