A specific and inexpensive assay of radiolabeled long-chain acyl-coenzyme A in isolated hepatocytes.
A simple procedure for determining radiolabeled long-chain acyl-CoA levels in isolated rat hepatocytes has been developed. It consists of a classical extraction of long-chain acyl-CoAs after preliminary removal of the excess labeled free fatty acid. A two-step TLC purification ensures elimination of long-chain acylcarnitine, the main interferent in most methods, and other common lipids. The purity of the acyl-CoA was confirmed by a second TLC system and by spectral analysis. Overall recovery was approximately 70%.
Stals
H K
HK
Katholieke Universiteit Leuven, Instituut voor Farmaceutische Wetenschappen, Belgium.
Declercq
P E
PE
D000214:Acyl Coenzyme A / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000302:isolation & purification; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D051381:Rats; D013053:Spectrophotometry
D000214:Acyl Coenzyme A; D005227:Fatty Acids