[Antimicrobial resistance in strains of Salmonella enterica isolated in the Hospital de Basurto (Bilbao) from 1987 to 1990].
BACKGROUND
Rate development of antimicrobial resistance of Salmonella strains in Hospital Basurto of Bilbao from 1987 to 1990 and to study their resistance mechanism.


METHODS
The antimicrobial resistance for all strains isolated (1201 strains) was performed by means of a agar-diffusion test. We selected 32 multi-resistant strains for additional study (MIC, conjugation, IEF, and plasmid profile).


RESULTS
The most frequent isolated serotypes were S. enteritidis (79.01%), S. typhimurium (8.5%) and Salmonella serogroup C1 (6.9%). The resistance to one or more of 17 antimicrobial rose significantly: 9.6% in 1987; 10.25% in 1988; 16.45% in 1989 and 13.73% in 1990. The percentage of resistant serotypes were: S. typhimurium (40.2%); Salmonella serogroup B (31.8%); Salmonella serogroup C1 (16.8%); Salmonella serogroup D1 (13.04%); Salmonella serogroup C2 (9.09%) and S. enteritidis (8.4%). In 27 multi-resistant strains and their transconjugants, beta-lactamase bands with a pl: 5.4 and/or 5.6 compatible with TEM-1 and/or TEM-2 were observed. Also, these strains carried a plasmid of high molecular weight (125 MD).


CONCLUSIONS
Although, the resistance of Salmonella is not a serious problem in our environment this situation is raising progressively with a greater number of strains with plasmid mediated beta-lactamases. So, the antimicrobial policy will be more severe and righ in both hospital and extrahospital surrounding.
Fernández de Aránguiz
A
A
Servicio de Microbiología, Hospital de Basurto, Bilbao, Vizcaya.
Alonso
R
R
Colom
K
K
Gallego
L
L
Umaran
A
A
Garaizar
J
J
Cisterna
R
R
D001426:Bacterial Proteins / Q000032:analysis; D003967:Diarrhea / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D004352:Drug Resistance, Microbial; D006761:Hospitals; D006801:Humans; D015994:Incidence; D011815:R Factors; D012480:Salmonella Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000145:classification / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D013030:Spain; D001618:beta-Lactamases / Q000032:analysis
D001426:Bacterial Proteins; D001618:beta-Lactamases
Resistencia antimicrobiana en cepas de Salmonella enterica aisladas en el Hospital de Basurto (Bilbao) desde 1987 hasta 1990.