Growth inhibition of N1E-115 mouse neuroblastoma cells by c-myc or N-myc antisense oligodeoxynucleotides causes limited differentiation but is not coupled to neurite formation.
Antisense oligodeoxynucleotides were found to be stable in the culture medium containing fetal calf serum (heat-inactivated 30 minutes at 65 degrees C) and in cells. Antisense oligomer treatment causes cessation of mitoses, but does not lead to morphological differentiation. Under antisense conditions, we have observed an increase in the amount of two neurospecific protein, namely peripherin and gamma-enolase. Comparison of the results obtained with chemical inducers and antisense oligodeoxynucleotides allows us to postulate three phases in N1E-115 differentiation: the first correspond to the arrest of mitosis, the second to the expression of a limited neuronal program, and the third to the morphological and electrophysiological differentiation.
Larcher
J C
JC
Laboratoire de Biochimie Cellulaire, URA 1115, Collège de France, Paris.
Basseville
M
M
Vayssiere
J L
JL
Cordeau-Lossouarn
L
L
Croizat
B
B
Gros
F
F
D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D016259:Genes, myc; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D007700:Kinetics; D008562:Membrane Glycoproteins; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D016501:Neurites / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009447:Neuroblastoma; D009479:Neuropeptides / Q000096:biosynthesis; D016376:Oligonucleotides, Antisense / Q000378:metabolism / Q000494:pharmacology*; D064531:Peripherins; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism
D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D016376:Oligonucleotides, Antisense; D064531:Peripherins; D010751:Phosphopyruvate Hydratase; D013936:Thymidine