Intracerebroventricular endothelin-1 (ET-1) produces Ca(2+)-mediated antinociception in mice.
Intracerebroventricular (i.c.v.) administration of endothelin-1 (ET-1) at doses of 0.313, 0.625, 1.25, 2.5 and 5 pmol/mouse produced a strong antinociceptive effect as evaluated by hot plate method in mice. The effect was dose-dependent and lasted for more than 120 min. The opioid receptor antagonist naloxone did not antagonize, and the cyclooxygenase inhibitors indomethacin and diclofenac slightly inhibited the ET-1 effect. The calcium overload blocker cinnarizine antagonized the antinociceptive effect of ET-1, suggesting that the ET-1 effect might be Ca(2+)-mediated.
Nikolov
R
R
Chemical Pharmaceutical Research Institute, Bulgarian Academy of Sciences, Sofia.
Maslarova
J
J
Semkova
I
I
Moyanova
S
S
D000698:Analgesia; D000818:Animals; D002118:Calcium / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000008:administration & dosage / Q000494:pharmacology*; D006358:Hot Temperature; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice
D016232:Endothelins; D002118:Calcium