The tomato 66.3-kD polyphenoloxidase gene: molecular identification and developmental expression.
A gene coding for a polypeptide abundant in tomato floral meristems was isolated and shown to represent a tomato 66.3-kD polyphenoloxidase. Analysis of cDNA clones and a corresponding intronless genomic clone indicated that the plastid-bound 587-residue-long polypeptide, designated P2, contains two conserved copper-binding domains, similar to those found in fungal and mammalian tyrosinases. P2 transcripts and polypeptides are accumulated in the arrested floral primordia of the anantha mutant inflorescences and are equally abundant in primordia of wild-type flowers; the gene continues to be expressed at high levels in developing floral organs. In young expanding leaves, P2 protein is concentrated in palisade cells and in epidermal trichomes. Expression patterns of P2 in plant meristems permit molecular distinction between floral and vegetative primordia, and, in a companion study, comparison with dUTPase suggests that the two genes mark two alternative complementary developmental programs in the floral and vegetative meristems of the tomato plants.
Shahar
T
T
Department of Biology, Technion-Israel Institute of Technology, Haifa.
Hennig
N
N
Gutfinger
T
T
Hareven
D
D
Lifschitz
E
E
D001483:Base Sequence; D004156:Catechol Oxidase / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000235:genetics; D063245:Plant Development; D010944:Plants / Q000201:enzymology* / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase