Joints formed by RecA protein from oligonucleotides and duplex DNA block initiation and elongation of transcription.
In the presence of the non-hydrolyzable analog of ATP, ATP gamma S, RecA protein can polymerize on an oligodeoxy-ribonucleotide to form a stable oligonucleoprotein filament that can find its homologous sequence in double-stranded DNA. The homologous joint formed by the oligonucleotide and duplex DNA is stable only if RecA protein is not removed. Such a nucleoprotein joint, covering a part or all of the promoter region of T3 or T7 phage RNA polymerase, blocked transcription directed by those polymerases. The same kind of joint, located downstream of the RNA polymerase promoter, also inhibited elongation of transcription and caused accumulation of truncated transcripts. These observations suggest that RecA protein can be used to shut off transcription from any promoter of known sequence.
Golub
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EI
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510.
Ward
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DC
Radding
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CM
D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D011938:Rec A Recombinases / Q000378:metabolism*; D013604:T-Phages / Q000201:enzymology*; D014158:Transcription, Genetic; D014764:Viral Proteins
D009838:Oligodeoxyribonucleotides; D014764:Viral Proteins; D004247:DNA; D011938:Rec A Recombinases; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases