Parkinsonism-inducing neurotoxin MPP+: uptake and toxicity in nonneuronal COS cells expressing dopamine transporter cDNA.
Expression of a cloned dopamine transporter complementary DNA in COS cells allows these primate kidney cells to accumulate the parkinsonism-inducing neurotoxin metabolite MPP+ (1-methyl-4-phenylpyridinium) avidly, and MPP+ toxicity results. By documenting that the dopamine transporter can confer MPP+ sensitivity to nonneural cells, these results highlight the key role that this transporter could play in mechanisms underlying parkinsonism.
Kitayama
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Laboratory of Molecular Neurobiology, Addiction Research Center/National Institute on Drug Abuse, Johns Hopkins University School of Medicine, Baltimore, MD. 21224.
Shimada
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Uhl
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GR
D015655:1-Methyl-4-phenylpyridinium / Q000493:pharmacokinetics* / Q000506:poisoning; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D050483:Dopamine Plasma Membrane Transport Proteins; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics; D009498:Neurotoxins / Q000493:pharmacokinetics / Q000506:poisoning; D010302:Parkinson Disease, Secondary / Q000139:chemically induced*
D002352:Carrier Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D009498:Neurotoxins; D004247:DNA; D015655:1-Methyl-4-phenylpyridinium