Baculovirus-expressed rabies virus M1 protein is not phosphorylated: it forms multiple complexes with expressed rabies N protein.
The rabies N, M1, M2, and G antigens have been expressed in Spodoptera frugiperda cells from single gene expression vectors or dual gene vectors (N/M1 or M2/G) using the baculovirus system. Although N protein was phosphorylated, no evidence for M1 phosphorylation was obtained. N-M1 complexes were formed in vivo using dual infections or the coexpression vectors, as well as in vitro in mixing experiments. The free or complexed rabies N and M1 proteins reacted with available monoclonal and polyclonal antibodies. By sedimentation analyses the N-M1 complexes were shown to exist in multiple configurations.
Préhaud
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NERC Institute of Virology and Environmental Microbiology, Oxford, UK.
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Bishop
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D016367:Baculoviridae / Q000235:genetics; D002213:Capsid / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003001:Cloning, Molecular; D005822:Genetic Vectors; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D010750:Phosphoproteins / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D011233:Precipitin Tests; D011820:Rabies virus / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D046911:Macromolecular Substances; D010750:Phosphoproteins; D011994:Recombinant Proteins; D014758:Viral Core Proteins