Use of delta-(alpha-aminoadipoyl) chromogenic amides in screening for aminoadipoyl amidohydrolases.
The synthesis of delta-(alpha-aminoadipoyl) aromatic amides and their use in screening for enzymes able to cleave delta-(alpha-aminoadipoyl) residues off the synthetic amides and cephalosporin C are described. A number of commercially available proteases and peptidases were not active with delta-(alpha-aminoadipoyl) chromogenic amides. Also, most tested microbial strains known to produce acylases did not hydrolyze these compounds. Only one microbial strain, Xanthomonas maltophila, had an appreciable activity toward the racemic form of chromogenic substrates. Activity measured in crude extracts from Xanthomonas cells indicated that this bacterium produces predominantly L-specific aminoadipoyl amidohydrolase and gamma-glutamyl hydrolase. A low level of cephalosporin C and glutaryl-cephalosporin acylase activities was also found.
Bouvrette
P
P
Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada.
Ziomek
E
E
D000272:Adipates / Q000138:chemical synthesis* / Q000378:metabolism; D000577:Amides / Q000138:chemical synthesis* / Q000378:metabolism; D000581:Amidohydrolases / Q000032:analysis* / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology*; D002510:Cephalosporinase / Q000378:metabolism; D002511:Cephalosporins / Q000138:chemical synthesis* / Q000378:metabolism; D007202:Indicators and Reagents; D007700:Kinetics; D015394:Molecular Structure; D013379:Substrate Specificity; D014974:Xanthomonas / Q000201:enzymology*
D000272:Adipates; D000577:Amides; D002511:Cephalosporins; D007202:Indicators and Reagents; C025163:cephalosporin C; D000581:Amidohydrolases; D002510:Cephalosporinase