Analysis of Vipera russelli venom using polyclonal antibodies prepared against its purified toxic phospholipase A2 VRV PL-V.
Vipera russelli venom induces predominantly neurotoxic, myotoxic necrotic and hemorrhagic symptoms in experimental animals and has several hydrolytic enzyme activities. In this study, V. russelli venom is characterized both as a PLA2 and as a toxin. Anti PL-V Ig (antibodies to a toxic phospholipase A2 VRV PL-V of V. russelli venom) nullifies the toxicity of whole V. russelli venom to a great extent. The neurotoxic symptoms vanish completely in the presence of anti PL-V Ig. The cross reacting components of whole V. russelli venom were removed by precipitating them from whole venom by the addition of anti PL-V Ig. The non-cross reacting components present in the supernatant were checked for toxicity. There was a significant reduction in toxicity. The LD50 value of the supernatant had increased from 4.1 mg/kg body weight to 11.7 mg/kg body weight and it showed about 34% of the total venom phospholipase A2 activity. It had edema forming, hemorrhagic and hemolytic activity but failed to induce neurotoxic, anticoagulant and myotoxic effects.
Kasturi
S
S
Department of studies in Biochemistry, University of Mysore, India.
Gowda
T V
TV
D000818:Animals; D000906:Antibodies / Q000276:immunology; D003429:Cross Reactions; D006470:Hemorrhage / Q000139:chemically induced; D051379:Mice; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism* / Q000633:toxicity; D054467:Phospholipases A2; D014757:Viper Venoms / Q000037:antagonists & inhibitors / Q000201:enzymology* / Q000276:immunology / Q000633:toxicity
D000906:Antibodies; D014757:Viper Venoms; D010741:Phospholipases A; D054467:Phospholipases A2