title: Magnetic bead purification of M13 DNA sequencing templates.
abstract: A method for the preparation of multiple M13 DNA sequencing templates is described. No phenol extraction is required and the procedure can be carried out in Eppendorf tubes or 96-well microtiter plates. Starting with a phage supernatant, the entire procedure is carried out in the same reaction vessel and all separation steps are based on a novel application of magnetic bead separation. The design of a 96-well magnetic separator is presented and the application of the method for large-scale sequencing is discussed.
authors.affiliation: Imperial Cancer Research Fund, London, England.
authors.forename: R P
authors.initials: RP
authors.lastname: Alderton
authors.forename: L M
authors.initials: LM
authors.lastname: Eccleston
authors.forename: R P
authors.initials: RP
authors.lastname: Howe
authors.forename: C A
authors.initials: CA
authors.lastname: Read
authors.forename: M A
authors.initials: MA
authors.lastname: Reeve
authors.forename: S
authors.initials: S
authors.lastname: Beck
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D004279:DNA, Viral / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D008280:Magnetics; D008863:Microspheres; D013698:Templates, Genetic
chemical_list: D004279:DNA, Viral