title: Replication of prophage P1 is cell-cycle specific.
abstract: P1 prophage replication during the Escherichia coli division cycle has been analyzed by using the membrane-elution technique to produce cells labelled at different times during the division cycle and scintillation counting for quantitative analysis of radioactive prophage DNA. P1 prophage replicates during a restricted portion of the bacterial division cycle, like the minichromosome, but at a time during the division cycle different than the time at which the minichromosome replicates in the same cell. A high-copy mini-R6K plasmid present in the same cell replicates throughout the division cycle. Over a wide range of growth rates, the P1 prophage replicates approximately one-half generation after the minichromosome replicates. Thus, the mechanisms underlying P1 replication are similar to those for the F plasmid and the chromosome. Replication occurs when some property related to cell size or cell mass reaches a constant value per origin.
authors.affiliation: Department of Chemical Engineering, University of Michigan, Ann Arbor 48109-2136.
authors.forename: J D
authors.initials: JD
authors.lastname: Keasling
authors.forename: B O
authors.initials: BO
authors.lastname: Palsson
authors.forename: S
authors.initials: S
authors.lastname: Cooper
mesh_terms: D002453:Cell Cycle; D003090:Coliphages / Q000235:genetics / Q000502:physiology*; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D007700:Kinetics; D010957:Plasmids; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D014316:Tritium; D013936:Thymidine