title: The sequential appearance of components of the synaptonemal complex during meiosis of the female rat.
abstract: This paper describes the light microscopy (LM) and electron microscopy (EM) localization of synaptonemal complex (SC) antigens in oocytes of rats. For this purpose, we used monoclonal antibodies (Mabs) that recognize components of 30 + 33, 125, and 190 kDa antigens of SCs of rat spermatocytes. The LM localization was performed by immunofluorescence and the EM localization by immunogold staining. The reaction of the Mabs with oocytes was similar to the reaction with spermatocytes, but weaker. The 30 + 33 kDa as well as the 190 kDa antigens could always be demonstrated if axial elements of the SC were present, irrespective of whether these were paired or unpaired. Thus, these antigens could be detected from leptotene--early zygotene until diplotene. The 190-kDa antigen appeared in a diffuse manner just before the appearance of the 30 + 33 kDa antigens. The 30 + 33 kDa antigens were not only detected in the axial elements of SCs but also in characteristic aggregates, which appeared in zygotene and persisted until after the SCs had disappeared. Such aggregates had rarely been observed in spermatocytes. The 125 kDa antigen was only present in the tripartite segments of SCs, at the inner edge of the lateral elements. Thus, the reaction of the Mab against the 125 kDa antigen was detectable in zygotene, pachytene, and very early diplotene. It appeared later than 30 + 33 kDa and 190 kDa antigens and it disappeared earlier. We found that several steps of the immunostaining procedure could cause variation in the intensity of the Mab reaction.
authors.affiliation: Institute of Human Genetics, University of Amsterdam, The Netherlands.
authors.forename: A J
authors.initials: AJ
authors.lastname: Dietrich
authors.forename: E
authors.initials: E
authors.lastname: Kok
authors.forename: H H
authors.initials: HH
authors.lastname: Offenberg
authors.forename: C
authors.initials: C
authors.lastname: Heyting
authors.forename: P
authors.initials: P
authors.lastname: de Boer
authors.forename: A C
authors.initials: AC
authors.lastname: Vink
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D008540:Meiosis; D016253:Microscopy, Immunoelectron; D009865:Oocytes / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D013090:Spermatocytes / Q000166:cytology; D013573:Synaptonemal Complex / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens