ATP synthesis in plasma membrane enriched particles by the action of insulin and related growth factors.
Early biosynthesis of short-life ATP was observed in plasma membranes of target cells stimulated by insulin or other polypeptide growth factors in the presence of all components of aerobic phosphorylation and cytochrome c. The effect is always mediated by the binding of insulin or growth factors to specific receptors. Erythrocyte plasma membranes are a convenient model to study the phenomenon. Insulin-stimulated synthesis of the plasma membrane "signal" ATP in an amount of 1-10 nM is potentized by ionophores carbonyl cyanide p-trifluorometoxyphenylhydrazone and monensin and inhibited by amiloride and ouabain. It is supposed that the plasma membrane "signal" ATP readily generated in response to a growth or mitogenic factor is an "amplifier" or "coupling agent" in the transduction of a signal to growth, proliferation, and mitogenesis. Biosynthesis of the plasma membrane "signal" ATP seems to be associated with partial reversion of Na+, K+ -ATPase with the participation of the plasma membrane redox chain as a proton generator.
Karelin
A A
AA
Laboratory of Biochemistry, Vishnevsky Institute of Surgery, Moscow.
Demidova
V S
VS
Globa
A G
AG
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D007328:Insulin / Q000502:physiology*; D008985:Monensin / Q000494:pharmacology; D009928:Organ Specificity; D010042:Ouabain / Q000494:pharmacology; D051381:Rats
D006133:Growth Substances; D007328:Insulin; D010042:Ouabain; D000584:Amiloride; D000255:Adenosine Triphosphate; D008985:Monensin