title: Identification, comparison and partial characterization of glycoproteins in the hemolymph of Schistosoma mansoni (Trematoda)-susceptible and resistant Biomphalaria glabrata (Gastropoda).
abstract: 1. Glycoproteins in the plasma (cell-free hemolymph) of Schistosoma mansoni-resistant and susceptible strains of Biomphalaria glabrata were identified, characterized and compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, electroblotting and a series of peroxidase-labeled lectins with different sugar specificities. 2. Schistosome-resistant snails possessed at least three glycoproteins (70, 116, 205 kDa) that were not present in plasma from susceptible snails. 3. Sporocysts (larval S. mansoni) preincubated in plasma from schistosome-resistant or susceptible B. glabrata adsorbed certain glycoproteins to their surface and some altered the binding of lectin probes to the parasite. 4. This study indicates that glycoproteins in the plasma of B. glabrata are associated with S. mansoni-susceptibility and resistance.
authors.affiliation: Division of Biological Sciences, University of Montana, Missoula 59812.
authors.forename: N J
authors.initials: NJ
authors.lastname: Vietri
authors.forename: W O
authors.initials: WO
authors.lastname: Granath
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000378:metabolism* / Q000469:parasitology; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism*; D006790:Host-Parasite Interactions; D012550:Schistosoma mansoni / Q000502:physiology
chemical_list: D006023:Glycoproteins