Colocalization of protein kinase C beta-subtype and calcitonin gene-related peptide in rat spinal cord.
Colocalization of calcitonin gene-related peptide (CGRP) and protein kinase C beta-subtype (PKC-beta) like immunoreactivities (LI) and the subcellular localization of CGRP-LI were studied in the ventral horn of rat spinal cord. Ultrastructurally CGRP-LI was localized on the membranes of the Golgi-complexes, in multivesicular bodies and in vesicles adjacent to the Golgi-complex in motoneuron perikarya. The colocalization of PKC-beta and CGRP-LI was detected in most of the ventral horn motoneurons. However, few motoneurons were only PKC-beta-immunoreactive. These results suggest that PKC-beta may be involved in the regulation of CGRP release from motoric axon terminals.
Hietanen-Peltola
M
M
Department of Biomedical Sciences, University of Tampere, Finland.
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology / Q000378:metabolism*; D006056:Golgi Apparatus / Q000201:enzymology; D008297:Male; D009046:Motor Neurons / Q000201:enzymology; D011493:Protein Kinase C / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology
D011493:Protein Kinase C; D015740:Calcitonin Gene-Related Peptide