title: Serologic screening for Trypanosoma cruzi among blood donors in central Brazil.
abstract: The study reported here compares results obtained by blood banks screening sera for chagasic (Trypanosoma cruzi) infection with results obtained by the Chagas' Disease Reference Laboratory of the Federal University of Goiás in Goiânia, Brazil. It also evaluates results obtained using the ELISA technique to screen the study sera. The survey used data from six of eight blood banks serving the city of Goiânia, an urban region of Central Brazil where Chagas' disease is highly endemic. The survey population consisted of 1,513 voluntary first-time blood donors whose donations occurred between October 1988 and April 1989. This group included 50% of all the first-time blood donors in that period. The six participating blood banks, which accounted for about 90% of all blood donations in Goiânia during the study period, routinely used indirect hemagglutination (IHA) and complement fixation (CF) tests to screen sera for antibodies to T. cruzi. Comparison of the results provided by the blood banks with the reference laboratory's results indicated a relative sensitivity of 77%, which ranged from 50% to 100% depending on the blood bank studied. The comparison, which found 12 false negative results, indicated that transfusions of infected blood might have occurred despite the serologic screening performed by the blood banks. Relative to the standard of positivity established for the study, the enzyme-linked immunosorbent assay (ELISA) technique was found to have a sensitivity of 96.3%. Considering as positive only those sera yielding positive IHA and indirect immunofluorescence (IIF) test results, the ELISA technique yielded 2 false negative and 41 false positive responses.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Brasil.
authors.forename: A L
authors.initials: AL
authors.lastname: de Andrade
authors.forename: C M
authors.initials: CM
authors.lastname: Martelli
authors.forename: A O
authors.initials: AO
authors.lastname: Luquetti
authors.forename: O S
authors.initials: OS
authors.lastname: de Oliveira
authors.forename: S
authors.initials: S
authors.lastname: Almeida e Silva
authors.forename: F
authors.initials: F
authors.lastname: Zicker
mesh_terms: D000328:Adult; D001771:Blood Banks / Q000706:statistics & numerical data; D001782:Blood Donors / Q000706:statistics & numerical data*; D001938:Brazil / Q000453:epidemiology; D014355:Chagas Disease / Q000097:blood / Q000453:epidemiology* / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005260:Female; D005455:Fluorescent Antibody Technique / Q000592:standards; D006386:Hemagglutination Tests / Q000592:standards; D006801:Humans; D008297:Male; D008403:Mass Screening; D015995:Prevalence; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies