title: Determination of the enantiomers of a novel 20,21-dinoreburnamenine derivative in rat plasma and brain by high-performance liquid chromatography using a chiral stationary phase.
abstract: A high-performance liquid chromatographic method with solid-phase extraction was developed for the assay of the enantiomers of a novel 20,21-dinoreburnamenine derivative (RU 49041) in rat plasma and brain using a chiral stationary phase (Nucleosil Chiral 2) and ultraviolet detection. The limit of detection was 10 ng/ml (or ng/g) in both tissues and the intra-assay precision was satisfactory (plasma, ca. 5%; brain, ca. 1%). The pharmacokinetic profiles of the two enantiomers were determined following oral administration of the racemate (10 mg/kg). The results show that their pharmacokinetics are very different: whereas both enantiomers appear in the brain, only the 3 alpha,16 beta-enantiomer is detected in plasma.
authors.affiliation: Centre de Recherches Roussel-Uclaf, Romainville, France.
authors.forename: P
authors.initials: P
authors.lastname: Vicat
authors.forename: D
authors.initials: D
authors.lastname: Cousty-Berlin
authors.forename: P
authors.initials: P
authors.lastname: Hunt
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D051381:Rats; D013237:Stereoisomerism; D014466:Ultraviolet Rays; D014748:Vinca Alkaloids / Q000032:analysis*
chemical_list: D014748:Vinca Alkaloids; C075611:11-methyl-20,21-dinoreburnamenine