title: Administration modalities and intestinal lymph absorption of arachidonic acid in rats.
abstract: We investigated the effects of the proportion of arachidonic acid on lymph lipoprotein synthesis in rats, when infused in the presence of lipids with varying degrees of saturation. A single dose of 90 mumol of lipid was administered intraduodenally and intestinal lymph was collected. Emulsion lipid constituents were 90 mumol of [14C]arachidonic acid alone, or either 30 mumol of [14C]arachidonic acid or 5 mumol of [14C]arachidonic acid and 25 mumol of linoleic acid, with 30 mumol of a free fatty acid and 30 mumol of a monoglyceride (the more saturated: oleic acid + monopalmitin; the more unsaturated: linoleic acid + monoolein). Radioactive lymph recovery of [14C]arachidonic acid infused alone reached 32.6 +/- 1.3% of the radioactivity administered and the presence of another fatty acid and a monoglyceride increased it from 38.7 +/- 2.6 to 51.9 +/- 1.1%. For 30 mumol of arachidonic acid, integration into lymph phospholipids was higher when arachidonic acid was infused with oleic acid and monopalmitin than with linoleic acid and monoolein (7.3 +/- 2.7 and 2.8 +/- 0.2% at the absorption peaks, respectively), and it was 4.8 +/- 0.5% when arachidonic acid was infused alone. For 5 mumol of arachidonic acid, the degree of unsaturation of the added lipid did not modify phospholipid incorporation, which was maintained at 6.9 to 7.2%. The proportion and size of chylomicrons increased with the degree of lipid emulsion unsaturation. Percentages of [14C]phospholipids and of chylomicrons were highly correlated. The proportion of arachidonic acid and degree of lipid emulsion unsaturation affected lymphatic arachidonic acid absorption modalities.
authors.affiliation: Département de Nutrition, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Université de Bourgogne, Dijon, France.
authors.forename: C
authors.initials: C
authors.lastname: Pavero
authors.forename: A
authors.initials: A
authors.lastname: Bernard
authors.forename: H
authors.initials: H
authors.lastname: Carlier
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000008:administration & dosage* / Q000378:metabolism*; D002914:Chylomicrons / Q000378:metabolism; D005227:Fatty Acids / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D007408:Intestinal Absorption; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008055:Lipids / Q000494:pharmacology; D008074:Lipoproteins / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008196:Lymph / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000378:metabolism
chemical_list: D002914:Chylomicrons; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D008055:Lipids; D008074:Lipoproteins; D008079:Lipoproteins, VLDL; D010743:Phospholipids; D014280:Triglycerides; D016718:Arachidonic Acid; D019787:Linoleic Acid