Induction of cytochrome P-450 by dimethyl sulfoxide in primary cultures of adult rat hepatocytes.
Treatment of hepatocyte cultures with dimethyl sulfoxide (DMSO) induced P-450IIE1-specific aniline 4-hydroxylase activity and P-450IA1-specific ethoxyresorufin O-deethylase activity at a concentration of 0.1% (v/v). The P-450IIB-specific pentoxyresorufin O-deethylase activity was induced only at the 2% (v/v) level. Dot blot analysis of the total cellular RNA and cycloheximide treatment of the culture suggested that induction of ethoxyresorufin O-deethylase activity by DMSO may be due to the increase of de novo synthesis of the P-450IA1 protein, not to accumulation of mRNA in the hepatocyte culture.
Jeong
T C
TC
Department of Life Science, Korea Advanced Institute of Science and Technology, Taejon.
Jeong
H G
HG
Yang
K H
KH
D000815:Aniline Hydroxylase / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010088:Oxidoreductases / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D003577:Cytochrome P-450 Enzyme System; D003513:Cycloheximide; D010088:Oxidoreductases; D000815:Aniline Hydroxylase; D019363:Cytochrome P-450 CYP1A1; D004121:Dimethyl Sulfoxide