Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons.
High efficiency gene transfer (greater than 90%) in chicken dorsal root ganglion neurons has been obtained by DNA calcium phosphate co-precipitation, hence providing an important tool to study control of gene expression in primary neurons. Transfection with c-fos promoter sequences linked to the chloramphenicol acetyltransferase reporter gene showed that the serum responsive element functions as a strong transcriptional enhancer. Transcription from this element is developmentally regulated, and mediates the genetic response to nerve growth factor (NGF) in developing avian sensory neurons. Furthermore, NGF exerts a negative effect on transcription from the cyclic AMP responsive element, thereby supporting the involvement of tyrosine kinase activation by NGF in primary sensory neurons.
Gabellini
N
N
Fidia Research Laboratories, Abano Terme, Italy.
Minozzi
M C
MC
Leon
A
A
Dal Toso
R
R
D000818:Animals; D001483:Base Sequence; D002130:Calcium Phosphates; D002478:Cells, Cultured; D011232:Chemical Precipitation; D002642:Chick Embryo; D002874:Chromosome Mapping; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005727:Ganglia, Spinal / Q000378:metabolism; D016762:Genes, fos / Q000235:genetics*; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009475:Neurons, Afferent / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection / Q000235:genetics*
D002130:Calcium Phosphates; D009414:Nerve Growth Factors; D011993:Recombinant Fusion Proteins; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous