title: Induction of mutations in the zebrafish with ultraviolet light.
abstract: Recessive lethal germline and specific locus somatic mutations were induced efficiently in the zebrafish by exposure of mature sperm to UV light. Mutagenesis of sperm yielded mosaic individuals: clones bearing novel mutations represented approximately 12-25% of the haploid germ cells and 25-50% of the somatic tissue. Simple methods are described for the reliable identification and propagation of newly arising developmental mutations in zebrafish.
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene 97403.
authors.forename: D J
authors.initials: DJ
authors.lastname: Grunwald
authors.forename: G
authors.initials: G
authors.lastname: Streisinger
mesh_terms: D000818:Animals; D004249:DNA Damage; D005260:Female; D005298:Fertility / Q000528:radiation effects; D005804:Genes, Lethal; D005838:Genotype; D008297:Male; D009030:Mosaicism; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D013094:Spermatozoa / Q000528:radiation effects*; D014466:Ultraviolet Rays; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*