Platelet-activating factor (PAF) plays an important role in the immediate asthmatic response in guinea-pig by augmenting the response to histamine.
1. To investigate the role of platelet activating factor (PAF) in the immediate asthmatic response, we examined the bronchial reactivity to histamine after administration of PAF to guinea-pigs or antigen challenge to passively sensitized guinea-pigs. 2. A bolus injection of PAF (20-40 ng kg-1), which did not cause a significant increase in intrathoracic pressure (ITP), augmented the bronchial response to histamine almost 8 fold. This airway hyperreactivity was observed even 1 min after PAF treatment. 3. A subthreshold dose of antigen (0.01 mg kg-1, i.v.) also provoked hyperreactivity to histamine, which became significant 6 and 11 min after the antigen treatment. 4. The specific PAF-antagonists, SM-10661 and CV-6209 (i.v.) dose-dependently inhibited both PAF- and antigen-induced airway hyperreactivities to histamine. 5. These results suggest that PAF plays an important role in antigen-induced acute airway responses by augmenting the activities of spasmogens.
Morooka
S
S
Research Laboratories, Sumitomo Pharmaceuticals Co., Ltd., Osaka, Japan.
Uchida
M
M
Imanishi
N
N
D000707:Anaphylaxis / Q000503:physiopathology; D000818:Animals; D001249:Asthma / Q000503:physiopathology*; D001980:Bronchi / Q000503:physiopathology; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D010047:Ovalbumin / Q000276:immunology / Q000502:physiology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D010976:Platelet Count / Q000187:drug effects; D011726:Pyridinium Compounds / Q000494:pharmacology; D012129:Respiratory Function Tests; D013189:SRS-A / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines
D010972:Platelet Activating Factor; D011726:Pyridinium Compounds; D013189:SRS-A; D013844:Thiazoles; D053778:Thiazolidines; C053321:CV 6209; C065776:3,5-dimethyl-2-(3-pyridyl)thiazolidin-4-one; D006632:Histamine; D010047:Ovalbumin