Induction of DNA repair synthesis in human monocytes/B-lymphocytes compared with T-lymphocytes after exposure to N-acetoxy-N-acetylaminofluorene and dimethylsulfate in vitro.
We have explored the induction of DNA repair synthesis in monocyte/B- and T-lymphocyte enriched cell fractions from 12 different human mononuclear blood cell populations. Unscheduled DNA synthesis was measured in monocyte/B- and T-cells after exposure to the DNA-damaging agents dimethylsulfate (DMS) and N-acetoxy-N-acetylaminofluorene in vitro. Also, the binding of DMS to DNA was measured. An increased DNA repair synthesis was measured in monocyte/B-lymphocytes after induction of the two different types of DNA lesions, whereas no induction of unscheduled DNA synthesis was observed in T-lymphocytes. A significantly higher DMS-DNA binding was also observed in monocyte/B-lymphocytes when compared with T-lymphocytes. Specific characterization of mononuclear blood cell populations used in biomonitoring of DNA adducts and repair is recommended.
Knudsen
L E
LE
Department of Toxicology and Biology, Danish National Institute of Occupational Health, Copenhagen.
Ryder
L P
LP
Wassermann
K
K
D000099:Acetoxyacetylaminofluorene / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects*; D006801:Humans; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D013463:Sulfuric Acid Esters / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology*
D013463:Sulfuric Acid Esters; D000099:Acetoxyacetylaminofluorene; C007482:dimethyl sulfate