High-performance liquid chromatography with electrochemical detection for the determination of levodopa, catecholamines and their metabolites in rat brain dialysates.
A high-performance liquid chromatographic assay with electrochemical detection is described for the simultaneous determination of levodopa, 3-O-methyldopa, dopamine, dihydroxyphenylacetic acid, homovanillic acid, 3-methoxytyramine, noradrenaline, adrenaline, 3-methoxy-4-hydroxyphenylethylene glycol and 5-hydroxyindoleacetic acid in rat brain dialysates. Samples are obtained in vivo using the microdialysis technique. Microdialysis probes are placed in the brain area to be studied and neurochemicals are collected by perfusion of the probe with modified Ringer's solution. Direct injection of the dialysates allows rapid and reliable results to be obtained.
Sarre
S
S
Department of Pharmaceutical Chemistry and Drug Analysis, Pharmaceutical Institute, Vrije Universiteit Brussel, Belgium.
Michotte
Y
Y
Herregodts
P
P
Deleu
D
D
De Klippel
N
N
Ebinger
G
G
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002395:Catecholamines / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism; D002421:Caudate Nucleus / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004295:Dihydroxyphenylalanine / Q000032:analysis; D004298:Dopamine / Q000032:analysis; D004837:Epinephrine / Q000032:analysis; D006719:Homovanillic Acid / Q000032:analysis; D007274:Injections, Intraperitoneal; D007980:Levodopa / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism; D008297:Male; D008750:Methyldopa / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013378:Substantia Nigra / Q000737:chemistry
D002395:Catecholamines; D015102:3,4-Dihydroxyphenylacetic Acid; D007980:Levodopa; D008750:Methyldopa; D004295:Dihydroxyphenylalanine; D004298:Dopamine; D009638:Norepinephrine; D006719:Homovanillic Acid; D004837:Epinephrine