title: Initiating a crystallographic study of a class II fructose-1,6-bisphosphate aldolase.
abstract: We have reproducibly crystallized the metal-dependent Class II fructose-1,6-bisphosphate aldolase from Escherichia coli. Crystals in the shape of truncated hexagonal bipyramids have unit cell dimensions of a = b = 78.4 A, c = 290.6 A and are suitable for a detailed structural analysis. The space group has been identified as P6(1)22 or enantiomorph. Data sets to approximately 2.9 A resolution have been recorded using both the Rigaku R-AXIS IIc image plate area detector coupled to a copper target rotating anode X-ray source and using the MAR image plate systems with synchrotron radiation at the EMBL outstation DESY in Hamburg, and at S.R.S. Daresbury. Diffraction beyond 2.5 A has been observed when large freshly grown crystals are used with the synchrotron beam. A data set to this resolution has been collected. Several putative heavy-atom derivative data sets have also been measured using synchrotron radiation facilities and analysis of these data sets is in progress.
authors.affiliation: Department of Chemistry, University of Manchester, U.K.
authors.forename: J H
authors.initials: JH
authors.lastname: Naismith
authors.forename: J D
authors.initials: JD
authors.lastname: Ferrara
authors.forename: S
authors.initials: S
authors.lastname: Bailey
authors.forename: K
authors.initials: K
authors.lastname: Marshall
authors.forename: Z
authors.initials: Z
authors.lastname: Dauter
authors.forename: K S
authors.initials: KS
authors.lastname: Wilson
authors.forename: J
authors.initials: J
authors.lastname: Habash
authors.forename: S J
authors.initials: SJ
authors.lastname: Harrop
authors.forename: A J
authors.initials: AJ
authors.lastname: Berry
authors.forename: W N
authors.initials: WN
authors.lastname: Hunter
mesh_terms: D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry* / Q000302:isolation & purification; D007527:Isoenzymes / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D007527:Isoenzymes; D005634:Fructose-Bisphosphate Aldolase