title: Rapid and simple gas chromatographic measurement of lactic acid in red blood cells, plasma, and tumor cells after hyperthermia.
abstract: The content of lactic acid in red blood cells, plasma, and Ehrlich ascites tumor cells were measured by a gas-liquid chromatography using a column with a terephtalic acid support coated with polyethylene glycol-6000. The lactic acid contents were directly determined in aqueous samples, because they were converted to a volatile derivative in the column. The method was rapid and simple, compared with previous methods which need time-consuming conversion of lactic acid to volatile derivatives. Our measurements showed the increase in the contents of intra- and extracellular lactic acid after hyperthermia.
authors.affiliation: Radioisotope Centre, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kageyama
authors.forename: Y
authors.initials: Y
authors.lastname: Onoyama
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: M
authors.initials: M
authors.lastname: Tumura
authors.forename: T
authors.initials: T
authors.lastname: Nishida
authors.forename: H
authors.initials: H
authors.lastname: Kogawa
authors.forename: K
authors.initials: K
authors.lastname: Tanabe
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism* / Q000473:pathology; D002849:Chromatography, Gas / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D006979:Hyperthermia, Induced; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D014407:Tumor Cells, Cultured
chemical_list: D007773:Lactates; D019344:Lactic Acid