Biodetermination of N-(deacetyl-O-4-vinblastoyl-23)-L-tryptophan, a metabolite of vintriptol, by high-performance liquid chromatography with fluorescence detection.
The determination of N-(deacetyl-O-4-vinblastoyl-23)-L-tryptophan (vintriptol acid, VtrpA), a metabolite of the investigational semi-synthetic vinca alkaloid vintriptol [N-(deacetyl-O-4-vinblastoyl-23)-L-ethyltryptophan, VtrpE], in plasma and urine samples is described. Sample pretreatment included liquid-liquid extraction of the buffered (pH 5.0) biological samples with chloroform-2-propanol (95:5, v/v). The analyses were performed by ion-exchange high-performance liquid chromatography on normal-phase silica with fluorescence detection. The assay was applied to the analysis of samples from cancer patients who had been treated with VtrpE in a phase I clinical study. VtrpA was found to be a principal metabolite of VtrpE with up to 1.2% of the administered dose excreted in the urine.
van Tellingen
O
O
Department of Clinical Chemistry, Netherlands Cancer Institute, Amsterdam.
Beijnen
J H
JH
Baurain
R
R
ten Bokkel Huinink
W W
WW
van der Woude
H R
HR
Nooyen
W J
WJ
D000970:Antineoplastic Agents / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000652:urine
D000970:Antineoplastic Agents; C050339:vintriptol; D014747:Vinblastine; C075450:vintriptol acid