Separation of flavonol-2-O-glycosides from Calendula officinalis and Sambucus nigra by high-performance liquid and micellar electrokinetic capillary chromatography.
Calendula officinalis and Sambucus nigra flowers were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC) and micellar electrokinetic capillary chromatography (MECC). RP-HPLC was performed on C8 Aquapore RP 300 columns with eluents containing 2-propanol and tetrahydrofuran. MECC was carried out on a 72-cm fused-silica capillary using sodium dodecyl sulphate and sodium borate (pH 8.3) as the running buffer. The results obtained by these techniques are compared.
Pietta
P
P
Università degli Studi di Milano, Italy.
Bruno
A
A
Mauri
P
P
Rava
A
A
D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D005419:Flavonoids / Q000302:isolation & purification*; D006027:Glycosides / Q000302:isolation & purification*; D008823:Micelles; D010944:Plants / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D005419:Flavonoids; D006027:Glycosides; D008823:Micelles