Corneal function after storage in dexsol or optisol.
Rabbit corneas were stored in Dexsol or Optisol (Chiron, Irvine, CA) for up to 2 wk at 4 degrees C. The thickness of corneas placed in Dexsol decreased 10 microns after they were placed initially in Dexsol, then increased approximately 8 microns/d for 7 d and 3 microns/d thereafter. Corneas placed in Optisol decreased 35 microns in thickness initially, then increased 2 microns/d thereafter. Human corneas showed similar thickness changes to those of the rabbit when stored in these media. After 5.5, 10, and 14 d in storage, rabbit corneas from each medium were cultured to assess their net deturgescence ability. Identical groups were cultured in media containing 20 microM ouabain to monitor the corneas' passive swelling characteristics. Corneas stored in either medium showed similar net deturgescence and passive swelling patterns after each storage period. Deturgescence rates decreased with increasing storage time, primarily because the rates of passive corneal swelling increased with storage time. Knowledge of the net deturgescence and passive swelling rates allowed an estimation of the total deturgescence activity of corneas after removal from Dexsol or Optisol. The total deturgescence activity of corneas stored in Dexsol for 5.5, 10, and 14 d was 85%, 68%, and 63% of control corneas, which were processed identically but not stored before culture. Corneas stored in Optisol exhibited 87%, 71%, and 69% of control deturgescence activity, respectively. These experiments show that Optisol was not significantly better than Dexsol in retaining poststorage corneal deturgescence activity but was superior to Dexsol in preventing corneal swelling during storage.
Walkenbach
R J
RJ
Missouri Lions Eye Research Foundation, University of Missouri School of Medicine, Columbia 65201.
Boney
F
F
Ye
G S
GS
D000368:Aged; D000818:Animals; D002809:Chondroitin Sulfates; D045424:Complex Mixtures; D003315:Cornea / Q000187:drug effects / Q000503:physiopathology*; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D003911:Dextrans; D005839:Gentamicins; D006531:HEPES; D006801:Humans; D009924:Organ Culture Techniques; D009926:Organ Preservation; D009930:Organic Chemicals; D011817:Rabbits
D045424:Complex Mixtures; D016895:Culture Media, Serum-Free; C075735:Dexsol; D003911:Dextrans; D005839:Gentamicins; D009930:Organic Chemicals; D002809:Chondroitin Sulfates; C075736:Optisol; D006531:HEPES