Gas chromatographic determination of urinary phenol conjugates after acid hydrolysis/extractive acetylation.
Phenolic metabolites of inhaled aromatic solvent vapours were liberated by acid hydrolysis of their urinary conjugates. Steam distillation enhanced by salting-out with MgSO4 gave good recoveries. After extractive acetylation, the derivatives of all cresols and xylenols were completely separated on a Se-54 capillary column. The overall recoveries of urinary phenols relative to the internal standard, 3-chlorophenol, were in the range 92-99%.
Weber
L
L
National Institute of Occupational Health, Budapest, Hungary.
D000107:Acetylation; D000395:Air Pollutants, Occupational / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010636:Phenols / Q000652:urine*
D000395:Air Pollutants, Occupational; D010636:Phenols