Simultaneous determination of chloroquine and its three metabolites in human plasma, whole blood and urine by ion-pair high-performance liquid chromatography.
A method was developed for the separation and measurement of chloroquine and three metabolites (desethylchloroquine, bisdesethylchloroquine and 4-amino-7-chloroquinoline) in biological samples by ion-pair high-performance liquid chromatography with UV detection. The method uses 2,3-diaminoaphthalene as an internal standard and provides a limit of detection between 1 and 2 ng/ml for chloroquine and its metabolites. The assay was linear in the range 12.5-250 ng/ml and the analytical recovery and reproducibility were sufficient. The assay was applied to the analysis of biological samples from a patient undergoing chloroquine chemoprophylaxis and a patient who had ingested chloroquine in a suicide attempt.
Houzé
P
P
Département de Biochimie-Pharmacologie-Toxicologie, C.H.R. de Versailles, Le Chesnay, France.
de Reynies
A
A
Baud
F J
FJ
Benatar
M F
MF
Pays
M
M
D002738:Chloroquine / Q000097:blood / Q000378:metabolism* / Q000506:poisoning / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007477:Ions; D011041:Poisoning / Q000378:metabolism; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D007477:Ions; D002738:Chloroquine