Isolation and characterization of the MHC linked beta-type proteasome subunit MC13 cDNA.
We have cloned and analysed the second mouse MHC-linked proteasome subunit, designated MC13, which appears to be homologous to the human RING10 proteasome protein. The isolated cDNA has an ORF encoding a protein of 276 amino acids with a molecular weight of ca. 30 kDa. Sequence alignment reveals that the subunit MC13 and several other mammalian proteasome subunits are encoded by a second proteasome gene family. This second gene family encodes subunits of the beta-type, reveals striking sequence similarities with the beta-subunit of archaebacterial proteasomes and is related to, but distinct from, the genes encoding the so-called alpha-type subunits.
Frentzel
S
S
Zentrum für Molekulare Biologie (ZMBH), Heidelberg, Germany.
Gräf
U
U
Hämmerling
G J
GJ
Kloetzel
P M
PM
D000595:Amino Acid Sequence; D000818:Animals; D001105:Archaea / Q000737:chemistry; D001483:Base Sequence; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D046911:Macromolecular Substances; D008285:Major Histocompatibility Complex / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics*; D046988:Proteasome Endopeptidase Complex; D012689:Sequence Homology, Nucleic Acid
D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D004247:DNA; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex