title: Substrate specificity of a CoA-dependent stearoyl transacylase from bovine testis membranes.
abstract: We identified a CoA-dependent stearoyl transacylase activity in bovine testis membranes, then examined the enzyme's specificity in mixed micelle systems containing the neutral detergent Triton X-100. The enzyme transferred stearoyl groups from a variety of phospholipids to sn-2-arachidonoyl lysophosphatidic acid (lysoPA), but showed very little palmitoyl transacylase activity. Its ability to transfer stearoyl groups was both donor- and acceptor-dependent. For example, it used weakly acidic phospholipids, such as sn-1-stearoyl-2-acyl species of phosphatidylinositol (PI), as donors, but did not use phosphatidylinositol-4,5-bisphosphate or sn-1-stearoyl-2-arachidonoyl phosphatidylcholine. Moreover, it used sn-2-acyl species of lysoPA and sn-2-arachidonoyl lysoPI as acceptors but did not use sn-2-arachidonoyl species of lysophosphatidylserine, lysophosphatidylethanolamine, or lysophosphatidylcholine. When taken together, our results raise the possibility that sn-1-stearoyl-2-acyl species of PI may be the primary acyl donors in the transacylase reaction in vivo, while sn-2-acyl species of lysoPA may be the primary acyl acceptors. Available evidence suggests that the PA that is formed may subsequently be converted into PI, but the metabolic fate of the other reaction product, sn-2-acyl lysoPI, remains to be determined.
authors.affiliation: Howard Hughes Medical Institute, Department of Medicine, University of Washington, Seattle 98195.
authors.forename: H
authors.initials: H
authors.lastname: Itabe
authors.forename: W C
authors.initials: WC
authors.lastname: King
authors.forename: C N
authors.initials: CN
authors.lastname: Reynolds
authors.forename: J A
authors.initials: JA
authors.lastname: Glomset
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D007700:Kinetics; D008297:Male; D008823:Micelles; D013379:Substrate Specificity; D013737:Testis / Q000201:enzymology*
chemical_list: D000214:Acyl Coenzyme A; D001095:Arachidonic Acids; D008823:Micelles; D000217:Acyltransferases; C075831:stearoyl transacylase