Simultaneous assay for amatoxins and phallotoxins in Amanita phalloides Fr. by high-performance liquid chromatography.
A reversed-phase high-performance liquid chromatographic method is described that allows the simultaneous determination of up to eight amatoxins and phallotoxins. The method identifies both neutral toxins (alpha- and gamma-amanitin, phalloidin, phallisin and phalloin) and acidic toxins (beta-amanitin, phallacidin and phallisacin). Toxins were separated, identified and determined by gradient elution with 0.02 M aqueous ammonium acetate-acetonitrile and simultaneous monitoring of the absorbances at 214 and 295 nm. The assay was successfully applied to the analysis of the toxins in a crude extract of Amanita phalloides. The limit of detection for each toxin was 10 ng/ml of extraction medium. The assay was further validated by analysing the toxin content in Galerina marginata, a species containing only amatoxins. This relatively simple method should be suitable for the detection of amatoxins and phallotoxins in almost any species of mushrooms.
Enjalbert
F
F
Laboratoire de Botanique, Phytochimie et Mycologie, Faculté de Pharmacie, Montpellier, France.
Gallion
C
C
Jehl
F
F
Monteil
H
H
Faulstich
H
H
D000545:Amanita / Q000737:chemistry*; D000546:Amanitins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011786:Quality Control
D000546:Amanitins; C018358:phallotoxin; C018207:amatoxin