Inhibition of proliferation by c-myb antisense RNA and oligodeoxynucleotides in transformed neuroectodermal cell lines.
Transfection of a neuroblastoma cell line with expression vectors containing two different segments of human c-myb complementary DNA in antisense orientation yielded far fewer transfectant clones than did the transfection with the identical segments in sense orientation. In cell clones expressing c-myb antisense RNA, levels of the c-myb protein were down-regulated and the proliferation rate was slower than that of cells transfected with sense constructs or the untransfected parental cell line. Treatment of neuroblastoma and neuroepithelioma cell lines with a c-myb antisense oligodeoxynucleotide strongly inhibited cell growth. These data indicate a definite involvement of c-myb in the proliferation of neuroectodermal tumor cells extending the role of this protooncogene beyond the hematopoietic system. The availability of cell clones that transcribe c-myb antisense RNA provides a useful tool to study the involvement of other genes in the proliferation and differentiation of neuroblastoma cells.
Raschellà
G
G
ENEA (Ente Nuove tecnologie Energia e Ambiente) CRE Casaccia, Division of Molecular Biology, Rome, Italy.
Negroni
A
A
Skorski
T
T
Pucci
S
S
Nieborowska-Skorska
M
M
Romeo
A
A
Calabretta
B
B
D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D009857:Oncogenes / Q000187:drug effects*; D016133:Polymerase Chain Reaction / Q000379:methods; D011519:Proto-Oncogenes; D016372:RNA, Antisense / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic; D014162:Transfection
D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D016372:RNA, Antisense; D012334:RNA, Neoplasm