title: Small-angle x-ray scattering study of metal ion-induced conformational changes in Serratia protease.
abstract: Metal ion-induced conformational changes in Serratia protease which contains one zinc ion per molecule were investigated by the small-angle x-ray scattering method. The molecule is an elongated ellipsoid of approximately 110 x 40 x 40 A with a large cleft in its central region. Comparisons of the native (zinc-enzyme) with the zinc-free (apoenzyme) enzyme and with the zinc-replated metalloenzyme show small but significant differences in their radii of gyration, maximum particle dimensions, and intraparticle pair-distance distributions. The radius of gyration and maximum particle dimension of the native enzyme are almost the same as those of the cobalt-enzyme but are shorter and longer, respectively, than those of the apo- and cadmium-enzymes. Simulation analysis based on the intraparticle pair-distribution function showed that these modified enzymes are comparable with the native enzyme in overall structure, and, except for the cobalt-enzyme, differ in cleft size. The residual enzymatic activity of the cobalt-enzyme is the same as that of the native enzyme, but the apo- and cadmium-enzymes have considerably less activity. The size of the cleft therefore is strictly controlled to ensure optimal enzyme activity, and the position and coordination behavior of the zinc ion in the cleft appears to be essential both for biological functioning and for the maintenance of the gross tertiary structure.
authors.affiliation: Hyogo Prefectural Institute of Industrial Research, Kobe, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Katsuya
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Katsube
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuura
authors.forename: K
authors.initials: K
authors.lastname: Tomoda
mesh_terms: D008666:Metalloendopeptidases / Q000737:chemistry* / Q000378:metabolism; D008670:Metals / Q000494:pharmacology; D008958:Models, Molecular; D011487:Protein Conformation; D012542:Scattering, Radiation; D012705:Serratia / Q000201:enzymology*; D014965:X-Rays; D015032:Zinc / Q000737:chemistry
chemical_list: D008670:Metals; D008666:Metalloendopeptidases; C044194:microbial metalloproteinases; D015032:Zinc