Warm Carolina rinse solution prevents graft failure from storage injury after orthotopic rat liver transplantation with arterialization.
An injury to nonparenchymal cells, characterized by loss of viability of sinusoidal endothelial cells and activation of Kupffer cells, occurs after reperfusion of livers stored for transplantation. Recently, a new solution, Carolina rinse solution, was shown to prevent reperfusion injury to endothelial cells in vitro almost completely and to improve graft survival after orthotopic rat liver transplantation (ORLT) without arterialization. ORLT with arterialization permits longer cold storage of donor livers and more closely models human surgery. Therefore, we evaluated the effects of Carolina rinse solution on graft survival after ORLT with arterialization in syngeneic Lewis rats. Just prior to implantation, donor livers stored in University of Wisconsin (UW) solution were rinsed with 30 ml of Ringer's solution, saline, or Carolina rinse solution at 1 degree-4 degrees C. In livers stored for 15 h and rinsed with Ringer's or saline solution, long-term graft survival was only 8%. Using Carolina rinse solution containing 1 mmol and 200 mumol adenosine per liter, graft survival improved to 40% and 80%, respectively. Graft survival did not improve when using Carolina rinse solution with adenosine omitted or Ringer's solution containing 200 mumol adenosine per liter. Livers were also rinsed with Carolina rinse solution containing 200 mumol adenosine per liter at 28 degrees-30 degrees C rather than at 1 degree-4 degrees C. With warm Carolina rinse solution, survival improved further to 100%, 80%, and 50% after 15, 18, and 21 h of storage. After 18 h of storage, light and electron microscopy demonstrated marked denudation of the sinusoidal lining and activation of Kupffer cells in grafts rinsed with Ringer's solution.(ABSTRACT TRUNCATED AT 250 WORDS)
Bachmann
S
S
Department of Cell Biology and Anatomy, School of Medicine, University of North Carolina, Chapel Hill 27599-7090.
Caldwell-Kenkel
J C
JC
Oleksy
I
I
Steffen
R
R
Thurman
R G
RG
Lemasters
J J
JJ
D000241:Adenosine; D000818:Animals; D005069:Evaluation Studies as Topic; D006085:Graft Survival; D066298:In Vitro Techniques; D008099:Liver / Q000098:blood supply / Q000293:injuries; D016031:Liver Transplantation / Q000379:methods / Q000473:pathology; D008297:Male; D008855:Microscopy, Electron, Scanning; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000517:prevention & control; D012996:Solutions; D013696:Temperature
C070887:Carolina rinse solution; D012996:Solutions; D000241:Adenosine