Effect of prostaglandins, inositol 1,4,5-trisphosphate, and phorbol esters on radiation-induced decreases in calcium influx in rat brain synaptosomes.
Gamma irradiation (60Co) reduced KCl-stimulated voltage-dependent uptake of 45Ca2+ in rat whole-brain synaptosomes. Prostaglandins, inositol 1,4,5-trisphosphate, and phorbol esters were tested for their ability to inhibit radiation-induced decreases in calcium influx. None of the compounds tested alone completely prevented radiation-induced decreases in calcium uptake, but some drug combinations did inhibit decreases. Results suggest that radiation-induced decreases in calcium uptake are due to impairment of protein kinase C activity and mobilization of calcium by these drugs.
Kandasamy
S B
SB
Behavioral Sciences Department, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20889-5145.
Harris
A H
AH
D000818:Animals; D001921:Brain / Q000166:cytology*; D002118:Calcium / Q000378:metabolism*; D003037:Cobalt Radioisotopes; D015237:Dinoprost / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D005720:Gamma Rays; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology*; D008297:Male; D010703:Phorbol Esters / Q000494:pharmacology*; D015230:Prostaglandin D2 / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*
D003037:Cobalt Radioisotopes; D010703:Phorbol Esters; D011453:Prostaglandins; D015544:Inositol 1,4,5-Trisphosphate; D015237:Dinoprost; D015232:Dinoprostone; D015230:Prostaglandin D2; D002118:Calcium