Reversed-phase high-performance liquid chromatography-thermospray mass spectrometry of radiation-induced decomposition products of thymine and thymidine.
High-performance liquid chromatography-thermospray mass spectrometry was applied to the analysis of various radiation-induced decomposition products of thymidine including N-(2-deoxy-beta-D-erythro-pentofuranosyl)formamide and the various diastereomers of 5,6-dihydroxy-5,6-dihydrothymidine, 5-hydroxy-5,6-dihydrothymidine and 5,6-dihydrothymidine. This method combines high sensitivity and product resolution, rendering it particularly useful for monitoring the formation of radiation-induced base damage within DNA.
Berger
M
M
Laboratoire Lésions des Acides Nucléiques, DRFMC/SESM, Centre d'Etudes Nucléaires, Grenoble, France.
Cadet
J
J
Berube
R
R
Langlois
R
R
van Lier
J E
JE
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D012680:Sensitivity and Specificity; D013936:Thymidine / Q000378:metabolism* / Q000528:radiation effects; D013941:Thymine / Q000378:metabolism* / Q000528:radiation effects
D013941:Thymine; D013936:Thymidine