Effects of precocenes on vitellogenesis in the adult female tick, Ornithodoros moubata (Acari: Argasidae).
Vitellogenin (Vg) concentrations in the hemolymph and ovarian development were studied in Ornithodoros moubata after treatment with precocenes 1 (P1) and 2 (P2). Precocene was dissolved in acetone or DMSO and topically applied to the dorsal surface of ticks: (1) at adult ecdysis; (2) 24 h before engorgement; (3) immediately after engorgement; and (4) 24 h after engorgement. Subsequently, P1 and P2 were dissolved in olive oil and injected through the gonopore into the body cavity 24 h after engorgement. Vitellogenin concentration was measured on days 5 and 10 after engorgement and ovarian development was scored on day 10, 20 or 30. Oviposition was also recorded and the average weight of eggs laid by females was determined. No differences in concentration of Vg in the hemolymph occurred between the control ticks and ticks treated topically or by injection with P1 and P2. Precocene did not suppress the synthesis of Vg in O. moubata. However, oviposition was reduced in ticks that survived repeated treatment with high doses of P2 dissolved in acetone.
Taylor
D
D
Department of Medical Zoology, School of Medicine, Mie University, Tsu, Japan.
Chinzei
Y
Y
Miura
K
K
Ando
K
K
D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D010058:Oviposition / Q000187:drug effects; D013987:Ticks / Q000502:physiology*; D014818:Vitellogenesis / Q000187:drug effects*; D014819:Vitellogenins / Q000032:analysis
D001578:Benzopyrans; D014819:Vitellogenins; C014163:precocene II; C019539:precocene I