Evaluation of a Salmonella-specific DNA probe by colony hybridization using non-isotopic and isotopic labeling.
A 2.3 kilobase (kb) Salmonella probe, JEO402-1, and two subfragments, F1214 (1.3 kb) and F1217 (0.8 kb), have been evaluated by colony hybridization using pure cultures of Salmonella serovars and non-salmonella bacteria. JEO402-1, and its subfragments, F1214 and F1217, hybridized to all of 156 different Salmonella serovars tested, while there was no reaction to 112 non-salmonella strains belonging to 19 genera and 37 species of Enterobacteriaceae. Together with previously published results, the JEO402-1 probe has now been shown to detect a total of 396 Salmonella strains belonging to 214 serovars of Salmonella subspecies I-VI. A total of 178 non-salmonella strains representing 23 genera and 51 species of Enterobacteriaceae have all tested negative with JEO402-1. The hybridization results obtained using a digoxigenin-labeled probe were similar to those obtained with 35S isotopic labeling when complete colony lysis was ensured.
Aabo
S
S
Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Denmark.
Thomas
A
A
Hall
M L
ML
Smith
H R
HR
Olsen
J E
JE
D015373:Bacterial Typing Techniques; D015342:DNA Probes / Q000235:genetics*; D004076:Digoxigenin; D005069:Evaluation Studies as Topic; D009693:Nucleic Acid Hybridization; D012475:Salmonella / Q000145:classification* / Q000235:genetics; D013455:Sulfur
D015342:DNA Probes; D013455:Sulfur; D004076:Digoxigenin