Biosynthesis of trypsinlike and chymotrypsinlike enzymes in immature Lutzomyia anthophora (Diptera: Psychodidae).
The biosynthesis of trypsinlike and chymotrypsinlike enzymes was followed in the four instars and pupa of Lutzomyia anthophora Addis. A 32-fold increase in the biosynthesis of trypsinlike enzymes was observed from the first to the fourth instar. Trypsinlike and chymotrypsinlike isozymes were also synthesized by pupae 1-8 d old. Similarly, a 29-fold increase in the biosynthesis of chymotrypsinlike isozymes also was observed from first to fourth instars. Several different [1,3-3H]DIP trypsinlike and chymotrypsinlike derivatives of first to fourth instars and pupae (1 and 8 d old) were studied using polyacrylamide gel electrophoresis and fluorography.
Mahmood
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F
Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Conn. 06510.
Borovsky
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D
D000818:Animals; D002918:Chymotrypsin / Q000096:biosynthesis*; D007303:Insect Vectors / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis*; D007814:Larva / Q000378:metabolism; D011576:Psychodidae / Q000201:enzymology*; D011679:Pupa / Q000378:metabolism; D014357:Trypsin / Q000096:biosynthesis*
D007527:Isoenzymes; D002918:Chymotrypsin; D014357:Trypsin