title: Characterization of a new picorna-like virus, himetobi P virus, in planthoppers.
abstract: Picorna-like virus particles, 29 nm in diameter, were purified from apparently healthy Laodelphax striatellus Fallen. The virus particles had a buoyant density of 1.352 g/ml in CsCl and a sedimentation coefficient of 161 s. The virus capsid proteins consisted of three major polypeptides of M(r)s 36,500, 33,000 and 28,000, and three minor polypeptides. The virus contained a major ssRNA of M(r) 2.8 x 10(6) and was also frequently associated with a minor dsRNA of M(r) 4 x 10(6). The 3' end of the ssRNA had a poly(A) tract of about 60 adenine residues. The virus has been provisionally named himetobi P virus.
authors.affiliation: National Institute of Agro-Environmental Sciences, Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Toriyama
authors.forename: P L
authors.initials: PL
authors.lastname: Guy
authors.forename: S
authors.initials: S
authors.lastname: Fuji
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D001483:Base Sequence; D004199:Disease Vectors; D007303:Insect Vectors / Q000382:microbiology*; D007313:Insecta / Q000382:microbiology*; D008969:Molecular Sequence Data; D012275:Oryza / Q000469:parasitology; D010849:Picornaviridae / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure; D012330:RNA, Double-Stranded / Q000737:chemistry; D014764:Viral Proteins / Q000032:analysis
chemical_list: D012330:RNA, Double-Stranded; D014764:Viral Proteins