The cytotoxicity, DNA crosslinking ability and DNA sequence selectivity of the aniline mustards melphalan, chlorambucil and 4-[bis(2-chloroethyl)amino] benzoic acid.
Three aniline derivatives melphalan (L-PAM), chlorambucil (CHL) and 4-[bis(2-chloroethyl)amino] benzoic acid (BAM) have been compared on the basis of their in vitro cytotoxicities, DNA interstrand crosslinking ability and DNA sequence selectivity. Cytotoxicity was assessed in the human colonic adenocarcinoma LS174T and leukaemic K562 cell lines using the sulpho-rhodamine B and tetrazolium dye reduction assays. The order of cytotoxicities was L-PAM greater than CHL greater than BAM in both cell lines with K562 being less sensitive than LS174T. This was different from the order CHL greater than L-PAM greater than BAM which would be predicted from simple chemical reactivity or rate of hydrolysis, parameters which have been used previously as indicators of biological potency for aromatic nitrogen mustards. DNA interstrand crosslinking in cells as determined by alkaline elution showed a correlation with IC50 values. The ranking order of activity was further predicted by the ability of the agents to produce interstrand crosslinks in isolated DNA. The extent of guanine N-7 alkylation, assessed using a modified DNA sequencing technique, mirrored cytotoxicity and crosslinking ability, but at equivalent levels of alkylation there was no significant difference in DNA sequence selectivity. These data demonstrates that simple chemical reactivity or hydrolysis rate is not a good indicator of DNA reactivity or cytotoxicity for a number of aniline mustards, whereas DNA interstrand crosslinking ability either measured directly in cells or in isolated DNA, gives a good indication of biological activity.
Sunters
A
A
Department of Medical Oncology, Charing Cross Hospital, London, U.K.
Springer
C J
CJ
Bagshawe
K D
KD
Souhami
R L
RL
Hartley
J A
JA
D010129:4-Aminobenzoic Acid / Q000494:pharmacology; D000478:Alkylation; D001665:Binding Sites; D002470:Cell Survival / Q000187:drug effects; D002699:Chlorambucil / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects*; D006801:Humans; D008558:Melphalan / Q000494:pharmacology*; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D062366:para-Aminobenzoates
D003432:Cross-Linking Reagents; D009588:Nitrogen Mustard Compounds; D062366:para-Aminobenzoates; C058699:4-N-bis(2-chloroethyl)aminobenzoic acid; D002699:Chlorambucil; D004247:DNA; D008558:Melphalan; D010129:4-Aminobenzoic Acid