title: Genetic and morphological characterization of an Escherichia coli chromosome segregation mutant.
abstract: The temperature-sensitive nucleoid segregation mutant of Escherichia coli, PAT32, formerly described as a parA mutant, has been shown to carry a mutation near 66 min on the genetic map. Fine mapping with phages from the collection of Kohara et al. is consistent with its being a parC allele. Observation by fluorescence microscopy revealed the formation, at a nonpermissive temperature, of filaments containing one or two large nucleoids and of normal-size anucleate cells. There was also a significant loss of viability.
authors.affiliation: Institut Jacques Monod, Centre National de la Recherche Scientifique, Université Paris 7, France.
authors.forename: P S
authors.initials: PS
authors.lastname: Stewart
authors.forename: R
authors.initials: R
authors.lastname: D'Ari
mesh_terms: D002455:Cell Division; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D005819:Genetic Markers; D009154:Mutation; D013696:Temperature
chemical_list: D005819:Genetic Markers