Determination of cicletanine enantiomers in plasma by high-performance capillary electrophoresis.
A sensitive and selective high-performance capillary electrophoresis procedure was developed for the determination of S(+) and R(-) enantiomers of cicletanine in human plasma. The procedure consisted in extraction of the drug with diethyl ether and analysis by micellar electrokinetic capillary chromatography in a fused-silica capillary using gamma-cyclodextrins in the run buffers and ultraviolet detection. The method was linear from 10 to 500 ng/ml and the limit of detection was 10 ng/ml for each enantiomer in plasma samples. The within-run precision of the method, expressed as relative standard deviation, was 10.4 and 9.6% at 25 ng/ml for S(+) and R(-) cicletanine, and 4.2 and 4.6% at 500 ng/ml, respectively. This method has been used to follow the time course of the concentrations of the cicletanine enantiomers in human plasma after a single therapeutic dose of cicletanine given by mouth.
Pruñonosa
J
J
Pharmacokinetic Department, S.A. Lasa Laboratorios, Barcelona, Spain.
Obach
R
R
Diez-Cascón
A
A
Gouesclou
L
L
D000959:Antihypertensive Agents / Q000097:blood* / Q000493:pharmacokinetics; D004232:Diuretics / Q000097:blood* / Q000493:pharmacokinetics; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D011725:Pyridines; D015203:Reproducibility of Results; D013237:Stereoisomerism
D000959:Antihypertensive Agents; D004232:Diuretics; D011725:Pyridines; C038068:cicletanine