[Charge distribution of serum IgA in IgA nephropathy with episodes of macrohematuria].
In order to examine further the role of the electrical change of IgA in the pathogenesis of IgAN, we studied the charge distribution of serum IgA in IgAN with episodes of macrohematuria. Blood samples were taken from 20 IgAN patients during bouts of macrohematuria and again more than 3 months later. Twenty healthy adults were used as controls. All samples were focused by Multiphor II flatbet electrofocusing unit apparatus (LKB) on an agarose gel (0.8% isogel agarose-EF, 2.5% pharmalyte 4.0-6.5). The focused gels were incubated with polyclonal goat anti-human IgA and were washed in several changes of saline for 48 hours. Immunofixed materials remaining in the gels after washing were fixed and stained with coomassie brilliant blue R250. The stained gels were analysed by densitometry. The relative areas of the individual peaks and their proportional contributions to the total IgA spectrotype were determined by computer. The results were as follows: 1. The percentage of proportional contribution of IgA peaks (pI 4.7-5.15) in IgAN were higher in patients than in controls (p less than 0.01). 2. The percentage of proportional contribution of IgA peaks (pI 4.9-5.05) in IgAN at bouts of macrohematuria was higher than in the same patients when not undergoing episodes of macrohematuria (p less than 0.01). These results suggest that the excess amount of anionic IgA may contribute to the pathogenesis of IgAN.
Sakata
H
H
Second Department of Internal Medicine, Nagasaki University School of Medicine.
D000328:Adult; D005260:Female; D005922:Glomerulonephritis, IGA / Q000150:complications* / Q000276:immunology; D006417:Hematuria / Q000209:etiology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged
D007070:Immunoglobulin A