Inhibition of Trichomonas vaginalis ornithine decarboxylase by amino acid analogs.
Ornithine decarboxylase (ODC) from Trichomonas vaginalis was inhibited irreversibly by several substrate analogs. Of these, DL-alpha-monofluoromethyldehydroornithine (MFMDO) and DL-alpha-monofluoromethylornithine (MFMO) were the most potent. The enzyme was unaffected by putrescine analogs suggesting that differences exist between the regulation of the trichomonad enzyme and that in other eukaryotes. In culture the ornithine analogs strongly inhibited putrescine synthesis and increased the generation time after 24 hr of exposure. In a semi-defined growth medium MFMDO methyl and ethyl esters increased the generation time from 4.5 hr to 9.0 and 8.2 hr, respectively. In standard undefined growth medium the trichomonad ODC was fully induced only after 15 hr (late log) and had an extended half-life of greater than 8 hr.
Yarlett
N
N
Haskins Laboratories, Pace University, New York, NY 10038.
Goldberg
B
B
Moharrami
M A
MA
Bacchi
C J
CJ
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D003470:Culture Media / Q000378:metabolism; D003513:Cycloheximide; D000518:Eflornithine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004795:Enzyme Stability; D007700:Kinetics; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000302:isolation & purification; D065108:Ornithine Decarboxylase Inhibitors; D011700:Putrescine / Q000378:metabolism; D014246:Trichomonas vaginalis / Q000201:enzymology*
D000596:Amino Acids; D000981:Antiprotozoal Agents; D003470:Culture Media; D065108:Ornithine Decarboxylase Inhibitors; C037000:2-fluoromethylornithine; D003513:Cycloheximide; D009955:Ornithine Decarboxylase; D011700:Putrescine; D000518:Eflornithine