title: Sequestered end products and enzyme regulation: the case of ornithine decarboxylase.
abstract: The polyamines (putrescine, spermidine, and spermine) are synthesized by almost all organisms and are universally required for normal growth. Ornithine decarboxylase (ODC), an initial enzyme of polyamine synthesis, is one of the most highly regulated enzymes of eucaryotic organisms. Unusual mechanisms have evolved to control ODC, including rapid, polyamine-mediated turnover of the enzyme and control of the synthetic rate of the protein without change of its mRNA level. The high amplitude of regulation and the rapid variation in the level of the protein led biochemists to infer that polyamines had special cellular roles and that cells maintained polyamine concentrations within narrow limits. This view was sustained in part because of our continuing uncertainty about the actual biochemical roles of polyamines. In this article, we challenge the view that ODC regulation is related to precise adjustment of polyamine levels. In no organism does ODC display allosteric feedback inhibition, and in three types of organism, bacteria, fungi, and mammals, the size of polyamine pools may vary radically without having a profound effect on growth. We suggest that the apparent stability of polyamine pools in unstressed cells is due to their being largely bound to cellular polyanions. We further speculate that allosteric feedback inhibition, if it existed, would be inappropriately responsive to changes in the small, freely diffusible polyamine pool. Instead, mechanisms that control the amount of the ODC protein have appeared in most organisms, and even these are triggered inappropriately by variation of the binding of polyamines to ionic binding sites. In fact, feedback inhibition of ODC might be maladaptive during hypoosmotic stress or at the onset of growth, when organisms appear to require rapid increases in the size of their cellular polyamine pools.
authors.affiliation: Department of Molecular Biology & Biochemistry, University of California, Irvine 92717.
authors.forename: R H
authors.initials: RH
authors.lastname: Davis
authors.forename: D R
authors.initials: DR
authors.lastname: Morris
authors.forename: P
authors.initials: P
authors.lastname: Coffino
mesh_terms: D000818:Animals; D001419:Bacteria / Q000201:enzymology*; D005658:Fungi / Q000201:enzymology*; D006801:Humans; D008322:Mammals / Q000378:metabolism*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*
chemical_list: D011073:Polyamines; D009955:Ornithine Decarboxylase