Characterization of the specific antigenicity of Mycobacterium fortuitum.
Mycobacterium fortuitum, biovar, fortuitum, the cause of serious skin and soft-tissue infections, can be differentiated from M. fortuitum, biovar. peregrinum, and other rapidly growing opportunistic mycobacteria by the presence of a unique antigenic glycolipid. The glycolipid is among the simplest of the acyl-trehalose-containing lipooligosaccharide class. The application of 1H and 13C NMR, methylation analysis, FAB/MS, and other procedures demonstrated the structure, beta-D-Glcp-(1----6)-2-O-acyl-alpha-D-Glcp-(1 in equilibrium with 1)-3,4,6-tri-O-acyl-alpha-D-Glcp. Thus, practically all environmental mycobacteria, many of them opportunistic pathogens, can be differentiated serologically and chemically on the basis of unique sugar arrangements within a few classes of glycolipids. The simplicity of the structure in M. fortuitum fortuitum combined with the distinct roughness of the parent strain raises the intriguing possibility that it is a spontaneous rough variant of the other mycobacteria with more elaborate glycolipids.
Besra
G S
GS
Department of Microbiology, Colorado State University, Fort Collins 80523.
McNeil
M R
MR
Brennan
P J
PJ
D000942:Antigens, Bacterial / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000276:immunology*; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000276:immunology*; D014312:Trisaccharides / Q000737:chemistry
D000942:Antigens, Bacterial; D006017:Glycolipids; D014312:Trisaccharides