Ultraviolet-B irradiation of platelets: a preliminary trial of efficacy.
Prior studies established that ultraviolet-B light (UVB) irradiation of platelet concentrates (PCs) at appropriate doses can eliminate the mixed lymphocyte culture-stimulating and -responding capacity of lymphocytes in the PCs without adversely affecting in vitro platelet function. The in vivo recovery and survival and in vitro characteristics of UVB-irradiated platelets were investigated in paired studies. PCs were stored for 1 day and then exposed to UVB. Platelet recovery, survival, and function were comparable to those of nonirradiated platelets. Recovery and survival of platelets stored for 5 days before UVB exposure were decreased relative to controls, although they were considered clinically acceptable. Paired transfusion studies were also performed in seven thrombocytopenic patients by using platelets obtained by apheresis. Comparable posttransfusion platelet increments and bleeding time corrections were obtained with both irradiated and control (nonirradiated) platelets. It can be concluded that platelets survive and function relatively normally in vivo after UVB irradiation sufficient to abolish lymphocyte reactivity in mixed lymphocyte culture. Long-term studies of UVB-irradiated PCs are needed to assess their potential in reducing recipient alloimmunization.
Sherman
L
L
Blood Center of Southeastern Wisconsin, Milwaukee.
Menitove
J
J
Kagen
L R
LR
Davisson
W
W
Lin
A
A
Aster
R H
RH
Buchholz
D H
DH
D016913:Blood Component Transfusion; D001792:Blood Platelets / Q000166:cytology / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D006801:Humans; D008214:Lymphocytes / Q000502:physiology; D010976:Platelet Count / Q000528:radiation effects; D013921:Thrombocytopenia / Q000503:physiopathology; D014466:Ultraviolet Rays