Measurement of trazodone in plasma and brain of rat by capillary gas chromatography with a nitrogen-selective detector.
A specific and highly sensitive method for the measurement of trazodone in plasma and brain of rat is presented. The compound and the internal standard were extracted from alkalinized samples with hexane and analysed by capillary gas chromatography with nitrogen-selective detection. The method was demonstrated to be accurate and precise. The limits of determination were 2 ng/ml for plasma and 24 ng/g for brain, which makes this procedure suitable for pharmacokinetic analysis.
Andriollo
O
O
Groupe de Recherches en Biodynamique du Médicament, Faculté de Pharmacie, Clermont-Ferrand, France.
Lartigue-Mattei
C
C
Chabard
J L
JL
Bargnoux
H
H
Petit
J
J
Berger
J A
JA
Pognat
J F
JF
D000818:Animals; D001923:Brain Chemistry; D002849:Chromatography, Gas / Q000379:methods*; D008297:Male; D009584:Nitrogen; D051381:Rats; D011919:Rats, Inbred Strains; D014196:Trazodone / Q000032:analysis* / Q000097:blood*
D009584:Nitrogen; D014196:Trazodone