title: Phosphotransfer signal transduction between two regulatory factors involved in the osmoregulated kdp operon in Escherichia coli.
abstract: The proteins KdpD and KdpE are regulatory factors critically involved in the osmotic regulation of the kdpABC operon that is responsible for a high-affinity transport system in Escherichia coli. In this study, we obtained biochemical evidence supporting the view that the KdpD protein is a sensory protein kinase that exhibits autophosphorylation and KdpE-phosphotransfer characteristics. During the course of such studies we established a procedure for purifying the KdpE protein in large quantities. We also developed a procedure for preparing cytoplasmic membrane enriched with the KdpD protein that exhibits in vitro ability with regard to phosphorylation of KdpE protein.
authors.affiliation: Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
authors.forename: A
authors.initials: A
authors.lastname: Sugiura
authors.forename: H
authors.initials: H
authors.lastname: Momoi
authors.forename: T
authors.initials: T
authors.lastname: Mizuno
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D008970:Molecular Weight; D009876:Operon; D009994:Osmolar Concentration; D010766:Phosphorylation; D011188:Potassium / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D015534:Trans-Activators; C075615:kdpE protein, E coli; C489580:KdpD protein, E coli; D011494:Protein Kinases; C075616:kdpD protein, Bacteria; D011188:Potassium