title: Stimulation of DNA synthesis in rat uterine cells by growth factors and uterine extracts.
abstract: Replicative DNA synthesis, as measured by thymidine incorporation, has been measured in rat uterine cells in primary culture in response to growth factors. Insulin, insulin-like growth factor-I (IGF-I), multiplication-stimulating activity (MSA) and platelet-derived growth factor (PDGF) stimulated DNA synthesis, while estradiol, epidermal growth factor (EGF), transforming growth factor-beta (TGF-beta), basic fibroblast growth factor (bFGF) and relaxin did not stimulate or did so weakly and only at very high concentrations. Uterine acid extracts also stimulated DNA synthesis. IGF-I stimulated at concentrations consistent with its acting through the IGF-I receptor; however, insulin stimulated at concentrations higher than expected for its acting through its receptor and this its action may be mediated through the IGF-I receptor. IGF-I was found in uterine tissue by radioimmunoassay (RIA). There was a 5- to 10-fold increase in IGF-I in the uteri from ovariectomized rats that had been treated with estradiol 24 h earlier. This is analogous to the increase in growth factor activity found previously in rat uterus after 24-h estradiol treatment (Beck, C.A. and Garner, C.W. (1989) Mol. Cell. Endocrinol. 63, 93-101). These data are consistent with the hypothesis that estradiol effects in the uterus are in part mediated through IGF-I.
authors.affiliation: Department of Biochemistry and Molecular Biology, Texas Tech University Health Sciences Center, Lubbock 79430.
authors.forename: C A
authors.initials: CA
authors.lastname: Beck
authors.forename: C W
authors.initials: CW
authors.lastname: Garner
mesh_terms: D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000032:analysis; D012065:Relaxin / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014599:Uterus / Q000378:metabolism*
chemical_list: D002457:Cell Extracts; D006133:Growth Substances; D007328:Insulin; D010982:Platelet-Derived Growth Factor; D011960:Receptors, Estrogen; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D012065:Relaxin; D013936:Thymidine