The specificity and efficiency of endogenous peptides in the induction of HLA class I alpha chain refolding.
The specificity and efficiency of endogenous peptides in the HLA class I binding have been investigated by the use of a simple procedure that is based on the serological detection of the folding of HLA class I alpha chains that is induced by the binding to specific peptides in the presence of beta 2 microglobulin. HLA class I proteins were solubilized with a nonionic detergent from cultured HLA homozygous B lymphoblastoid cells and dissociated by alkaline denaturation. The resulting unfolded alpha chains were isolated by gel filtration at a neutral pH. The unfolded alpha chains showed a high refolding capacity and specificity when tested in the presence of an excess beta 2 microglobulin against endogenous peptides extracted by alkaline or acid treatment from cultured B lymphoblastoid cells of various HLA class I phenotypes. Cells of identical or overlapping HLA phenotypes clearly showed shared peptides, whereas such peptide sharing was rarely, if at all, seen between cells of non-overlapping HLA phenotypes. The efficiency of endogenous peptides in the induction of refolding was high; at an estimated concentration of 0.2 microM or less, a strong refolding effect was observed.
Tanigaki
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Department of Molecular Immunology, Roswell Park Memorial Institute, Buffalo, NY 14263.
D000483:Alleles; D015395:Histocompatibility Antigens Class I / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000502:physiology*; D011487:Protein Conformation; D011489:Protein Denaturation; D001613:beta 2-Microglobulin / Q000494:pharmacology
D015395:Histocompatibility Antigens Class I; D010455:Peptides; D001613:beta 2-Microglobulin