title: Reversed-phase high-performance liquid chromatography-thermospray mass spectrometry of radiation-induced decomposition products of thymine and thymidine.
abstract: High-performance liquid chromatography-thermospray mass spectrometry was applied to the analysis of various radiation-induced decomposition products of thymidine including N-(2-deoxy-beta-D-erythro-pentofuranosyl)formamide and the various diastereomers of 5,6-dihydroxy-5,6-dihydrothymidine, 5-hydroxy-5,6-dihydrothymidine and 5,6-dihydrothymidine. This method combines high sensitivity and product resolution, rendering it particularly useful for monitoring the formation of radiation-induced base damage within DNA.
authors.affiliation: Laboratoire Lésions des Acides Nucléiques, DRFMC/SESM, Centre d'Etudes Nucléaires, Grenoble, France.
authors.forename: M
authors.initials: M
authors.lastname: Berger
authors.forename: J
authors.initials: J
authors.lastname: Cadet
authors.forename: R
authors.initials: R
authors.lastname: Berube
authors.forename: R
authors.initials: R
authors.lastname: Langlois
authors.forename: J E
authors.initials: JE
authors.lastname: van Lier
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D012680:Sensitivity and Specificity; D013936:Thymidine / Q000378:metabolism* / Q000528:radiation effects; D013941:Thymine / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D013941:Thymine; D013936:Thymidine