[Morphologic and cytochemical study of Sertoli cell nuclei in ruminants].
Nuclei and nucleoli were examined at an ultrastructural level in the testicular tissue of bulls and rams. In the two species there occurs a morphologically interesting so called multivesicular nuclear body; the interpretation of its function is not quite clear. The tissue was processed by a routine electron microscope technique and than a cytochemical demonstration of several types of proteins was used. Acidic argyrophilic proteins were demonstrated by a silver-staining technique, basic lysine-rich proteins by means of ethanolic PTA, and ribonucleoproteins (RNP) by the method of Bernhard's regressive preferential staining. Multivesicular nuclear bodies in bull Sertoli's cells are composed of a greater number of membrane bound vesicles with granules on the outer surface (Fig. 1). The results of cytochemistry reactions show that the granules contain acidic argyrophilic proteins (Fig. 3) and RNP (Fig. 7). The intervesicular material is an analogy of the dense fibrillar component of normal nucleoli of somatic cells. This component contains the three types of investigated proteins (Figs. 2, 3, 5, 7). In the nuclear bodies of ram Sertoli's cells the number of vesicles is much lower, the fibrillar component is prevailing, in form of clusters and striae of filamentous material around the vesicles. The cytochemistry reactions proved that this material contains acidic argyrophilic proteins (Fig. 4) and basic PTA positive (Fig. 6) proteins. The presence of acidic and basic regulatory proteins in the fibrillar component of multivesicular nuclear bodies indicates the active synthesis of RNA occurring in this material. It is therefore possible to consider this special type of nuclear bodies as a full equivalent of nucleoli of somatic cells as to the function.
Antalíková
L
L
Research Institute of Animal Production, Praha-Uhrínĕves.
Rozinek
J
J
D000818:Animals; D002417:Cattle / Q000033:anatomy & histology*; D002466:Cell Nucleolus / Q000737:chemistry / Q000648:ultrastructure; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure*; D006651:Histocytochemistry; D008297:Male; D008840:Microfilament Proteins / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis; D012708:Sertoli Cells / Q000737:chemistry / Q000648:ultrastructure*; D012756:Sheep / Q000033:anatomy & histology*
D008840:Microfilament Proteins; D009687:Nuclear Proteins
Morfologicko-cytochemické studium jadérek Sertoliho bunĕk prezvýkavců.