A simple method of concentrating monoclonal antibodies from culture supernatant by ultrafiltration.
How to obtain concentrated monoclonal antibodies from culture supernatant is of great importance to many scientists today. In this report, a simple, single-step technique is described to concentrate monoclonal antibodies directly from hybridoma-culture supernatant. The contaminants can be removed from the supernatant by using an ultrafiltration unit which allows macromolecules of less than 100,000 MW to pass through leaving behind monoclonal antibodies which is purified and concentrated more than 25-fold.
Saha
K
K
University of Texas, M.D. Anderson Cancer Center, Science Park-Research Division, Smithville 78957.
Case
R
R
Wong
P K
PK
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D006825:Hybridomas; D066298:In Vitro Techniques; D014462:Ultrafiltration
D000911:Antibodies, Monoclonal; D003470:Culture Media