title: Peptide display on filamentous phage capsids. A new powerful tool to study protein-ligand interaction.
abstract: Peptides can be displayed on the surface of filamentous bacteriophages by fusion to phage coat proteins. It was recently shown that vast (10(8)) collections of phages, each exposing a variant of the original peptide, can be constructed and utilized as a general source of peptide ligands. By panning these libraries on a target molecule linked to a solid support it is possible to select, out of the hundreds of millions of clones, those few phages that display a peptide that binds the target molecule. Searching these libraries is a powerful tool to be applied in many areas of fundamental and applied biology.
authors.affiliation: Department of Biology, University of Tor Vergata, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Cesareni
mesh_terms: D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism*; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D015698:Genomic Library; D008024:Ligands; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D008024:Ligands; D011994:Recombinant Proteins