Analysis of the transcripts of cytochrome P-450IID gene subfamily in bovine adrenal gland.
An in situ hybridization experiment showed that the gene transcripts of P-450IID subfamily were uniformly distributed in bovine liver cells and at low levels in bovine adrenal cortex and medulla. There was no clear localization of transcripts of P-450IID genes in three adrenocortical zones, zona glomerulosa, zona fasciculata and zona reticularis. The intensity of signal obtained in the adrenal cortex and medulla showed approximately 2.5:1 ratio. The presence of gene transcripts of P-450IID subfamily in adrenocortex was confirmed by amplifying cDNA using PCR and hybridizing the PCR products with P-450IID cDNA.
Matsuo
Y
Y
Department of Biochemistry, Kagawa Medical School, Japan.
Iwahashi
K
K
Kawai
Y
Y
Ichikawa
Y
Y
D000311:Adrenal Glands / Q000201:enzymology*; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D003577:Cytochrome P-450 Enzyme System