Analysis of muscarinic receptor subtypes in the mouse cochlea by means of the polymerase chain reaction.
Total RNA was extracted with guanidine thiocyanate from the cochleas of 16-day-old CBAJ mice. The mRNA was purified from the total RNA using oligo-dT cellulose, and the mRNA was treated with DNase to degrade genomic DNA. After reverse transcription, resulting cDNA was amplified by polymerase chain reaction (PCR), using primers specific for the nucleotide sequences m1-m5, representing subtypes of muscarinic acetylcholine receptors. PCR products corresponding to subtypes m1, m3, and m5, but not to m2 and m4, were amplified. These results suggest that muscarinic acetylcholine receptors of these odd-numbered subtypes are expressed in the mammalian cochlea.
Drescher
D G
DG
Department of Otolaryngology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Upadhyay
S
S
Wilcox
E
E
Fex
J
J
D000818:Animals; D001483:Base Sequence; D003051:Cochlea / Q000737:chemistry / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis / Q000235:genetics; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011976:Receptors, Muscarinic / Q000032:analysis* / Q000737:chemistry / Q000235:genetics
D012333:RNA, Messenger; D011976:Receptors, Muscarinic; D004247:DNA