Quantitative analysis of platelet-activating factor in rat brain.
Age-related decrease of the platelet-activating factor (PAF) content in rat brain was shown by a convenient method consisting of solid extraction of lipids with a Sep-Pak C-18 cartridge, lipid separation by HPLC and bioassay on rabbit platelets. This method was sufficiently sensitive to allow measurement of PAF in a single brain, and the recovery of PAF was quite high throughout the procedure.
Tokumura
A
A
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Yotsumoto
T
T
Hoshikawa
T
T
Tanaka
T
T
Tsukatani
H
H
D000375:Aging / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D010972:Platelet Activating Factor / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D010972:Platelet Activating Factor