Alternative splicing is responsible for the presence of two tissue factor mRNA species in LPS stimulated human monocytes.
Although normally absent from the surface of all circulating cell types, tissue factor (TF) can be induced to appear on circulating monocytes by stimulants like bacterial lipopolysaccharide (LPS) and phorbolesters. Northern analysis of RNA isolated from LPS stimulated human monocytes demonstrates the presence of 2.2 kb and 3.1 kb TF mRNA species. The 2.2 kb message codes for the TF protein. As demonstrated by Northern blot analysis with a variety of TF gene probes, the 3.1 kb message arises from an alternative splicing process which fails to remove 955 bp from intron 1. Because of a stop codon in intron 1 no TF protein is produced from the 3.1 kb transcript. This larger transcript should therefore not be taken into account when comparing TF gene transcription and TF protein levels.
van der Logt
C P
CP
Haemostasis and Thrombosis Research Unit, University Hospital, Leiden, The Netherlands.
Reitsma
P H
PH
Bertina
R M
RM
D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003062:Codon / Q000187:drug effects; D015342:DNA Probes; D015894:Genome, Human; D006801:Humans; D007438:Introns / Q000187:drug effects; D008070:Lipopolysaccharides / Q000633:toxicity*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects*; D012326:RNA Splicing / Q000187:drug effects*; D012333:RNA, Messenger / Q000032:analysis*; D013925:Thromboplastin / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
D003062:Codon; D015342:DNA Probes; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D013925:Thromboplastin