Biochemical studies on 2-deoxy-scyllo-inosose, an early intermediate in the biosynthesis of 2-deoxystreptamine. II. Quantitative analysis of 2-deoxy-scyllo-inosose.
A practical quantitative analysis of 2-deoxy-scyllo-inosose by means of GC-MS selected-ion monitoring (SIM) was exploited in order to assay the enzymatic 2-deoxy-scyllo-inosose formation, the first stage of the 2-deoxystreptamine biosynthesis. Mass spectral fragmentations of 2-deoxy-scyllo-inosose tetra-O-trimethylsilyl (TMS) ether was also investigated by the use of [2,2-2H2]-2-deoxy-scyllo-inosose.
Yamauchi
N
N
Department of Chemistry, Tokyo Institute of Technology, Japan.
Kakinuma
K
K
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002478:Cells, Cultured; D008401:Gas Chromatography-Mass Spectrometry; D006595:Hexosamines / Q000096:biosynthesis; D007294:Inositol / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D013302:Streptomyces / Q000378:metabolism
D000900:Anti-Bacterial Agents; D006595:Hexosamines; D007294:Inositol; C075548:2-deoxyinosose; C007265:2-deoxystreptamine