title: Comparison of the distribution of carbonic anhydrase isozymes (CA-I, CA-II, CA-III) in the rat gastrointestinal tract.
abstract: The present paper described the immunohistochemical distributions of carbonic anhydrase (CA) isozymes. CA-I, CA-II and CA-III, in the epithelium lining the rat gastrointestinal tract, with rabbit antibodies to equine CA-I, CA-II and CA-III. Prior to the immunohistochemical examinations, the crossreactivities of these antibodies to the rat-antigens were confirmed in this study. In the stomach, surface epithelial cells and parietal cells of the glandular region showed an immunoreactivity only to CA-II. In the large intestine, each immunoreactivity to CA isozyme (CA-I, CA-II and CA-III) was localized in the upper portion of intestinal glands, and decreased toward the distal digestive tract, but absent in the small intestine. The present histological findings suggested that the CA isozymes might play a role in the ion-transportation during the water absorption in the rat large intestine.
authors.affiliation: Toxicology Research Laboratories, Chugai Pharmaceutical Co., Ltd., Nagano, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Igarashi
authors.forename: Y
authors.initials: Y
authors.lastname: Kano
authors.forename: T
authors.initials: T
authors.lastname: Nishita
authors.forename: H
authors.initials: H
authors.lastname: Amasaki
authors.forename: M
authors.initials: M
authors.lastname: Asari
mesh_terms: D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis*; D003429:Cross Reactions; D004064:Digestive System / Q000201:enzymology*; D004848:Epithelium / Q000201:enzymology; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains / Q000378:metabolism*
chemical_list: D002256:Carbonic Anhydrases