Halothane and isoflurane inhibit endothelium-dependent relaxation elicited by acetylcholine.
The purpose of this study was to determine whether volatile anesthetics modify the release of endothelium-derived relaxing factor. We examined the effects of halothane and isoflurane on endothelium-dependent relaxation and 3',5'-cyclic guanosine monophosphate formation elicited by acetylcholine and ionophore A23187 in isolated rat aorta. Halothane and isoflurane (1%-2%) significantly attenuated acetylcholine-induced relaxation of the phenylephrine-contracted aorta but had no significant effect on relaxation induced by A23187, nitroprusside, and nitroglycerin. Basal and A23187 (10(-7) M)-stimulated levels of 3',5'-cyclic guanosine monophosphate were slightly lowered by halothane and isoflurane (2%). In contrast, the increase of 3',5'-cyclic guanosine monophosphate elicited by acetylcholine (10(-5) M) was significantly attenuated by halothane (2%) and abolished by isoflurane (2%). These findings indicate that halothane and isoflurane strongly inhibit the release of endothelium-derived relaxing factor elicited by acetylcholine.
Toda
H
H
Department of Anesthesia, Kyoto University Hospital, Japan.
Nakamura
K
K
Hatano
Y
Y
Nishiwada
M
M
Kakuyama
M
M
Mori
K
K
D000109:Acetylcholine / Q000037:antagonists & inhibitors*; D000818:Animals; D000001:Calcimycin / Q000037:antagonists & inhibitors; D006221:Halothane / Q000494:pharmacology*; D007530:Isoflurane / Q000494:pharmacology*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D005996:Nitroglycerin / Q000037:antagonists & inhibitors; D009599:Nitroprusside; D051381:Rats; D011919:Rats, Inbred Strains
D009599:Nitroprusside; D009569:Nitric Oxide; D000001:Calcimycin; D007530:Isoflurane; D005996:Nitroglycerin; D000109:Acetylcholine; D006221:Halothane