The 13C NMR study of the binding of gold(I) thiomalate with ergothionine in aqueous solution.
The interaction of gold(I) thiomalate (Autm) (Myocrysine) with ergothionine (ErSH) has been studied in aqueous solution at pH 7.4. It was found that ErSH forms a ternary complex of the type ErS-Au-tm at a 1:1 mole ratio; unlike other thiols (e.g., cysteine and glutathione) it does not eject thiomalate (Htm) as a free ligand. However, in the presence of glutathione (GtSH), both ligands, ErSH as well as Htm, from the ErS-Au-tm complex were freed. The 13C NMR chemical shifts of C-2 resonance of ergothionine in the presence of Autm shifts greater than imidazolidine-2-thione (Imt) and 1,3-Diazinane-2-thione (Diaz), indicating the stronger complexation of ErS-Au-tm compared to Imt-Au-tm and Diaz-Au-tm.
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King Fahd University of Petroleum and Minerals, Dhahran, Saudi Arabia.
D002247:Carbon Isotopes; D004880:Ergothioneine / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry; D006052:Gold Sodium Thiomalate / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D012709:Serum Albumin / Q000737:chemistry; D012996:Solutions
D002247:Carbon Isotopes; D008024:Ligands; D012709:Serum Albumin; D012996:Solutions; D006052:Gold Sodium Thiomalate; D004880:Ergothioneine; D005978:Glutathione