Novel glycosylation-defective baby hamster kidney cells.
The plant lectin wheat germ agglutinin (WGA) has previously been used to select more than ten different glycosylation-defective phenotypes in a variety of mammalian somatic cells. Three WGA-resistant phenotypes have now been obtained spontaneously from baby hamster kidney (BHK) cells. These mutant BHK cells exhibit a pattern of cross resistance and sensitivity to multiple plant lectins, suggesting that the cell surface carbohydrates of these cells are altered. Two WGA-resistant BHK phenotypes appear similar to WGA-resistant CHO cells that lack terminal sialic acid and galactose residues on their cell surface carbohydrates. The third WGA-resistant BHK cell phenotype has not previously been seen in WGA-resistant mammalian cells.
Ripka
J
J
Department of Medicine, University of Miami School of Medicine, FL 33101.
Pierce
M
M
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002999:Clone Cells; D006224:Cricetinae; D004351:Drug Resistance / Q000235:genetics; D005700:Galactosyltransferases / Q000378:metabolism*; D006001:Glycoconjugates / Q000032:analysis / Q000378:metabolism*; D006031:Glycosylation; D007668:Kidney; D037102:Lectins / Q000633:toxicity*; D010641:Phenotype; D014909:Wheat Germ Agglutinins / Q000633:toxicity*
D006001:Glycoconjugates; D037102:Lectins; D014909:Wheat Germ Agglutinins; D005700:Galactosyltransferases