Cloning of the gamma chain of the human IL-2 receptor.
A third subunit, the gamma chain, of the human interleukin-2 receptor (IL-2R) was identified, and a complementary DNA clone encoding this member of the cytokine receptor family was isolated. The gamma chain is necessary for the formation of the high- and intermediate-affinity receptors, which consists of alpha beta gamma heterotrimers and beta gamma heterodimers, respectively. The IL-2R on murine fibroblastoid cells can be internalized after binding IL-2 only if the gamma chain is present; alpha and beta are insufficient for internalization. Thus, the gamma chain is an indispensable component of the functional IL-2R.
Takeshita
T
T
Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan.
Asao
H
H
Ohtani
K
K
Ishii
N
N
Kumaki
S
S
Tanaka
N
N
Munakata
H
H
Nakamura
M
M
Sugamura
K
K
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
D007376:Interleukin-2; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2