A novel technique for the preparation of osmotically stabilized and permeabilized cells of extremely halophilic bacteria.
The cells of Haloferax mediterannei were stabilized by cross-linking with 0.5% glutaraldehyde for 10 min. Such cells were found to be osmotically stable even when suspended in water. The stabilized cells could be permeabilized by treatment with chloroform without leakage of intracellular components. No significant difference in the properties of an intracellular enzyme aldolase was observed, using either cell-free extract or the osmotically stabilized and permeabilized cells. This novel technique can serve as a useful tool for studying in situ regulatory characteristics of intracellular functions in halobacteria and can also help in their re-use under more stabilized conditions for biotechnological applications.
D'Souza
S E
SE
Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Altekar
W
W
D'Souza
S F
SF
D002463:Cell Membrane Permeability / Q000502:physiology*; D003432:Cross-Linking Reagents; D004120:Dimethyl Suberimidate; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005976:Glutaral; D006217:Halobacterium / Q000166:cytology*; D016591:Histocytological Preparation Techniques; D009997:Osmotic Pressure
D003432:Cross-Linking Reagents; D004120:Dimethyl Suberimidate; D005634:Fructose-Bisphosphate Aldolase; D005976:Glutaral