Interaction of cardiac alpha-actinin and actin in the presence of doxorubicin.
The therapeutic use of doxorubicin (an antitumoral antibiotic belonging to the anthracycline group) is limited by its cardiotoxicity. Adriamycin (DXR) causes myocardial subcellular damage, such as myocytolysis, disarray of actin filaments, and alterations in the Z-band with loss of sarcomeric organization. We studied the effect of stoichiometrical concentrations of DXR on the interaction between cardiac actin and alpha-actinin in solution. Doxorubicin inhibits the formation of alpha-actinin/actin tridimensional networks and bundles. The main effect of the drug seems to be on the size of the actin polymers.
DalleDonne
I
I
Department of Biology, University of Milan, Italy.
Milzani
A
A
Contini
P
P
Bernardini
G
G
Colombo
R
R
D008841:Actin Cytoskeleton / Q000737:chemistry / Q000187:drug effects; D000185:Actinin / Q000032:analysis / Q000494:pharmacology*; D000199:Actins / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D004347:Drug Interactions / Q000502:physiology; D006321:Heart / Q000187:drug effects; D009206:Myocardium / Q000737:chemistry*; D011108:Polymers; D014461:Ultracentrifugation
D000199:Actins; D011108:Polymers; D000185:Actinin; D004317:Doxorubicin