Presence of a repressor protein for testis-specific H2B (TH2B) histone gene in early stages of spermatogenesis.
The rat testis-specific TH2B histone gene assumes a hypomethylated chromatin structure at all stages of spermatogenesis. However, the TH2B mRNA level is very low in pre-meiotic spermatogenic cells and rises sharply in meiotic pachytene spermatocytes. The low level of TH2B mRNA in pre-meiotic spermatogenic cells appears to be a result of transcriptional repression of the gene by a pre-meiotic cell-specific protein which binds to a site between the TATA element and the transcription initiation site of TH2B gene.
Lim
K
K
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599-7260.
Chae
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CB
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000502:physiology; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D006657:Histones / Q000235:genetics*; D008297:Male; D008540:Meiosis; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012097:Repressor Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012708:Sertoli Cells / Q000502:physiology*; D013090:Spermatocytes / Q000502:physiology*; D013091:Spermatogenesis / Q000502:physiology*; D014158:Transcription, Genetic
D006657:Histones; D012333:RNA, Messenger; D012097:Repressor Proteins; D004247:DNA