title: Isolation of a cytopathic virus from weak pigs on farms with a history of swine infertility and respiratory syndrome.
abstract: Severe clinical signs of swine infertility and respiratory syndrome (SIRS) of unknown cause were observed in several Minnesota swine farms between November 1990 and March 1991. Forty-five lung samples of weak pigs were collected from 13 swine farms, and virus isolation was attempted using swine alveolar macrophage (SAM) cultures. A cytopathic virus was isolated from 19 lung samples collected from 6 different farms. Four pregnant sows were infected intranasally with a tissue suspension from which virus was isolated, and 4 6-week-old pigs and 2 contact pigs were infected intranasally with 1 of the isolates. The 4 sows farrowed 12 stillborn and 32 normal pigs. Virus was recovered from 10 of 19 pigs examined. Infected 6-week-old pigs were clinically normal except for slightly elevated rectal temperatures and mild respiratory signs. No or mild interstitial pneumonic lesions were observed in inoculated pigs, but the lesion was obvious in the 2 contact pigs. Seroconversion was observed in sows and pigs as measured by indirect fluorescent antibody (IFA). Serologic identification of the isolates was carried out by IFA using reference serum prepared from an experimentally infected sow. A cytoplasmic fluorescence was observed on the SAM monolayers infected with each of the 19 different isolates. Fluorescence was also observed when the monolayers were tested with SIRS virus ATCC VR-2332-infected sow sera. Replication of the isolates was not affected in the medium containing 5-iodo-2'-deoxyuridine but was inhibited by treatment with ether. The isolates were relatively stable at 56 C and did not agglutinate with various erythrocytes tested.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
authors.forename: I J
authors.initials: IJ
authors.lastname: Yoon
authors.forename: H S
authors.initials: HS
authors.lastname: Joo
authors.forename: W T
authors.initials: WT
authors.lastname: Christianson
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: J E
authors.initials: JE
authors.lastname: Collins
authors.forename: J H
authors.initials: JH
authors.lastname: Carlson
authors.forename: S A
authors.initials: SA
authors.lastname: Dee
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D005260:Female; D005313:Fetal Death / Q000382:microbiology / Q000662:veterinary; D005455:Fluorescent Antibody Technique; D007247:Infertility, Female / Q000382:microbiology / Q000662:veterinary*; D008168:Lung / Q000382:microbiology / Q000473:pathology; D016676:Macrophages, Alveolar / Q000382:microbiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology / Q000662:veterinary; D012328:RNA Viruses / Q000302:isolation & purification* / Q000502:physiology; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D013577:Syndrome; D014777:Virus Diseases / Q000382:microbiology / Q000662:veterinary*