title: Rapid isolation with Sep-Pak C18 cartridges and capillary gas chromatography of triazine herbicides in human body fluids.
abstract: A simple and rapid method, for the isolation of eight triazine herbicides from human serum and urine, using Sep-Pak C18 cartridges is presented. After mixing with distilled water, serum and urine samples containing the herbicides, were loaded on Sep-Pak C18 cartridges and eluted with either chloroform only or chloroform/methanol (9:1). The herbicides were detected by capillary gas chromatography with both flame ionization detection (FID) and nitrogen-phosphorus detection (NPD). Separation of eight triazine herbicides from each other and from impurities was generally satisfactory with the use of a non-polar DB-1 capillary column. Recovery of most compounds was excellent for both chloroform and chloroform/methanol (9:1) as elution solvents. Backgrounds were cleaner and evaporation time was shorter for the chloroform only than for the chloroform/methanol (9:1). The NPD gave sensitivity more than 10-20 times higher than that of FID.
authors.affiliation: Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kumazawa
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Seno
authors.forename: O
authors.initials: O
authors.lastname: Suzuki
mesh_terms: D002849:Chromatography, Gas; D005410:Flame Ionization; D006540:Herbicides / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D014227:Triazines / Q000097:blood / Q000302:isolation & purification* / Q000652:urine
chemical_list: D006540:Herbicides; D014227:Triazines