Characterization of lipopolysaccharides from four Pasteurella haemolytica serotype strains: evidence for presence of sialic acid in serotypes 1 and 5.
Highly purified lipopolysaccharides (LPS) obtained from four strains of Pasteurella haemolytica representative of four different serotypes were studied to ascertain their overall structural elements and sugar and fatty acid compositions. SDS-PAGE analysis revealed that each LPS was of the smooth-type although they differed in migration patterns. Somewhat unusual features of these LPS included the presence of: (a) rhamnose in the core oligosaccharides of serotypes 2 and 3; and (b) sialic acid in the LPS of serotypes 1 and 5. The fatty acids, myristic, hydroxymyristic and palmitic occur in essentially equivalent amounts in each of these LPS. In addition, stearic acid was present in small amounts of serotypes 1 and 5.
Utley
S R
SR
Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Bhat
U R
UR
Byrd
W
W
Kadis
S
S
D000606:Amino Sugars / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D016978:Mannheimia haemolytica / Q000737:chemistry* / Q000145:classification; D009005:Monosaccharides / Q000032:analysis; D019158:N-Acetylneuraminic Acid; D012703:Serotyping; D012794:Sialic Acids / Q000032:analysis*
D000606:Amino Sugars; D005227:Fatty Acids; D008070:Lipopolysaccharides; D009005:Monosaccharides; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid