title: Fetal fracture healing in a lamb model.
abstract: A large animal model to assess fetal fracture repair and the ability to close excisional bony defects is presented. Incisional and excisional ulnar fractures were made in 14 midgestation fetal lambs, harvested at serial time points, and subjected to high-resolution low-kilovolt magnification radiographs, magnetic resonance imaging scans, and histologic analysis. Fetal fracture healing was characterized by early closure of excisional defects and rapid fracture healing with minimal or no soft-tissue inflammation or callus formation. Magnetic resonance imaging scans of the fractures revealed a characteristic pattern compatible with the histologic findings, namely, minimal inflammation in soft tissue adjacent to the fracture site. Histologic and magnification radiographic findings indicated that complete bony repair occurred within 21 days in incisional defects and within 40 days in excisional defects. In both cases, healed fetal bone resembled normal bone matrix. Excisional defects, including periosteum, of greater than three times the width of the bony cortex closed rapidly with virtually normal-appearing bony matrix and with minimal or no callus formation.
authors.affiliation: Fetal Treatment Program, University of California, San Francisco.
authors.forename: M T
authors.initials: MT
authors.lastname: Longaker
authors.forename: B R
authors.initials: BR
authors.lastname: Moelleken
authors.forename: J C
authors.initials: JC
authors.lastname: Cheng
authors.forename: R W
authors.initials: RW
authors.lastname: Jennings
authors.forename: N S
authors.initials: NS
authors.lastname: Adzick
authors.forename: J
authors.initials: J
authors.lastname: Mintorovich
authors.forename: D G
authors.initials: DG
authors.lastname: Levinsohn
authors.forename: L
authors.initials: L
authors.lastname: Gordon
authors.forename: M R
authors.initials: MR
authors.lastname: Harrison
authors.forename: D J
authors.initials: DJ
authors.lastname: Simmons
mesh_terms: D000818:Animals; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis / Q000473:pathology / Q000503:physiopathology*; D050723:Fractures, Bone / Q000175:diagnosis / Q000473:pathology / Q000503:physiopathology*; D008279:Magnetic Resonance Imaging; D010027:Osteotomy; D012756:Sheep; D014458:Ulna Fractures / Q000175:diagnosis / Q000473:pathology / Q000503:physiopathology; D014945:Wound Healing