TLC separation of certain tetracycline and amino glycopeptide antibiotics.
The separation of tetracycline and amino glycopeptide antibiotics was achieved on silica gel thin layers. Tetracycline antibiotics were resolved on a Co+2 (1.0%) impregnated silica gel layer using ethanol:acetic acid:water (10:6:6, v/v/v) as the mobile phase. Amino glycopeptide antibiotics were separated on an untreated silica gel layer using the mobile phase n-butanol:formic acid:water (6:5:7, v/v/v). The spots of these antibiotics were located by exposing the chromatoplate to iodine vapours.
Bhushan
R
R
Department of Chemistry, University of Roorkee, India.
Ali
I
I
D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer; D013754:Tetracyclines / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D013754:Tetracyclines