Photogeneration of NADH under coupled action of CdS semiconductor and hydrogenase from Alcaligenes eutrophus without exogenous mediators.
Photoreduction of NAD has been accomplished by a system consisting of the NAD-dependent hydrogenase from Alcaligenes eutrophus immobilized on CdS particles with formate as artificial electron donor. Enzymatically active NADH is formed under illumination of this system by visible light. Accumulation of the coenzyme dimer (NAD)2 was not detected. NAD photoreduction is supposed to proceed via the direct electron transfer from the semiconductor to the enzyme electron transport chain. However, NADH formation as a result of hydrogenase interaction with anion-radicals (CO2.-) formed in the course of formate photooxidation cannot at present be excluded.
Shumilin
I A
IA
A.N. Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow.
Nikandrov
V V
VV
Popov
V O
VO
Krasnovsky
A A
AA
D000421:Alcaligenes / Q000201:enzymology*; D002104:Cadmium / Q000737:chemistry*; D019187:Cadmium Compounds; D006863:Hydrogen-Ion Concentration; D006864:Hydrogenase / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D010777:Photochemistry; D013440:Sulfides
D019187:Cadmium Compounds; D013440:Sulfides; D002104:Cadmium; C034939:cadmium sulfide; D009243:NAD; D006864:Hydrogenase