API Listeria, a new and promising one-day system to identify Listeria isolates.
API Listeria is a new 10-test strip for 24-h biochemical identification of Listeria isolates. With this commercial system, 85% of 646 Listeria strains, including atypical isolates selected for this study, were recognized at the species and subspecies level without a complementary test. A new test differentiates Listeria monocytogenes from L. innocua on the basis of the absence of arylamidase from the former. With this system, 97.7% (252 of 258) of the L. monocytogenes strains tested were correctly identified and differentiated from 99.4% (175 of 176) of the L. innocua strains also tested. Gram-positive bacteria other than Listeria spp. gave quite different biochemical patterns. This system considerably reduced the time needed for conventional identification, since results were available within 18 to 24 h.
Bille
J
J
Centre National de Référence des Listeria, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
Catimel
B
B
Bannerman
E
E
Jacquet
C
C
Yersin
M N
MN
Caniaux
I
I
Monget
D
D
Rocourt
J
J
D001431:Bacteriological Techniques; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000175:diagnosis; D006094:Gram-Positive Bacteria / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D008087:Listeria / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D008089:Listeria monocytogenes / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D008088:Listeriosis / Q000175:diagnosis; D013997:Time Factors