[Studies on the effect of combination of amphotericin B and flucytosine on Candida albicans by flow cytometry].
The antifungal effects of amphotericin B (AMPH) and flucytosine (5-FC) on three strains of Candida albicans were studied by flow cytometry (FCM). When the IFM4954 and IFM4949 were treated with AMPH or 5-FC alone, cytograms of cell distribution changed at the concentration of 1/2 MIC, respectively. Next, the effect of combination of AMPH and 5-FC were examined. Cytogram of cell distribution of IFM4954 and IFM4949 changed under the combination of 1/4 MIC of AMPH and 1/4 MIC of 5-FC, namely a synergic effect was observed. In conclusion, FCM is a useful apparatus for analysis of the effect of antifungal agents.
Taguchi
H
H
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University.
Tanaka
R
R
Miyaji
M
M
Nishimura
K
K
Kanno
H
H
Ishiyama
H
H
D000666:Amphotericin B / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D004359:Drug Therapy, Combination; D005434:Flow Cytometry; D005437:Flucytosine / Q000494:pharmacology*; D008722:Methods
D000666:Amphotericin B; D005437:Flucytosine