title: A reticulocyte-binding protein complex of Plasmodium vivax merozoites.
abstract: Plasmodium vivax merozoites primarily invade reticulocytes. The basis of this restricted host cell preference has been debated. Here we introduce two novel P. vivax proteins that comigrate on reducing SDS-polyacrylamide gels, colocalize at the apical pole of merozoites, and adhere specifically to reticulocytes. The genes encoding these proteins, P. vivax reticulocyte-binding proteins 1 and 2 (PvRBP-1 and PvRBP-2), have been cloned and analyzed. Homologous genes are evident in the closely related simian malaria parasite, P. cynomolgi, which also prefers to invade reticulocytes, but are not evident in the genome of another related simian malaria parasite, P. knowlesi, which invades all red blood cell subpopulations. Native PvRBP-1 is likely a transmembrane-anchored disulfide-linked protein, and along with PvRBP-2 may function as an adhesive protein complex. We propose that the RBPs of P. vivax, and homologous proteins of P. cynomolgi, function to target the reticulocyte subpopulation of red blood cells for invasion.
authors.affiliation: Department of Medical and Molecular Parasitology, New York University Medical Center, New York 10010.
authors.forename: M R
authors.initials: MR
authors.lastname: Galinski
authors.forename: C C
authors.initials: CC
authors.lastname: Medina
authors.forename: P
authors.initials: P
authors.lastname: Ingravallo
authors.forename: J W
authors.initials: JW
authors.lastname: Barnwell
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D016790:Plasmodium knowlesi / Q000235:genetics; D010966:Plasmodium vivax / Q000235:genetics* / Q000469:parasitology; D015800:Protozoan Proteins / Q000032:analysis* / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D015800:Protozoan Proteins; C075483:reticulocyte-binding protein, Plasmodium vivax