Comparison of copper binding components in dog serum with those in other species.
The copper content of dog serum and its distribution to copper binding proteins was compared with that of rat and mouse. Total serum Cu concentrations of dogs and mice were one third those of the rat. Plasma ceruloplasmin, determined by azide-inhibitable oxidase activity with two substrates, was 8-fold less in the dog and 9- to 20-fold less in the mouse than in the rat, and, in both dogs and mice, there was 70-75% less ceruloplasmin Cu, determined by atomic absorption after gel filtration. In the dog, the largest proportion of total and exchangeable serum Cu was with the transcuprein fraction. Only one third as much Cu was with albumin in the dog (and mouse) versus the rat, and this was released much more readily through dialysis. In dogs and mice, the exchangeable (nonceruloplasmin) serum copper pool was half the size of that in rats and humans. Especially in the mouse (but also in rats and dogs), a small proportion of the exchangeable pool appeared bound to ferroxidase II. We conclude that the dog may rely more on transcuprein and low molecular weight complexes and less on albumin and ceruloplasmin for transport of copper to cells.
Montaser
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A
Department of Chemistry and Biochemistry, California State University, Fullerton 92634.
Tetreault
C
C
Linder
M
M
D000328:Adult; D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D002570:Ceruloplasmin / Q000378:metabolism; D003300:Copper / Q000097:blood*; D004285:Dogs; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010088:Oxidoreductases / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism; D013045:Species Specificity
D002352:Carrier Proteins; D012709:Serum Albumin; C023548:copper-binding protein; D003300:Copper; D010088:Oxidoreductases; C023100:ferroxidase II; D002570:Ceruloplasmin