A comparison of hepatic glucokinase gene expression in high- and low-activity strains of mice.
1. Compared with the rat, mouse liver glucokinase activities show a different sensitivity to changes in insulin concentrations. When animals from a high glucokinase-high insulin level strain C3H/He are crossed with those from a low glucokinase-low insulin strain C58, individuals from recombinant inbred lines show non-parental phenotypes with high glucokinase activity but low insulin and vice versa. 2. Messenger RNA levels are greater in high-enzyme-activity mice strains than in low-activity animals, suggesting that differences in either transcription of the glucokinase gene or in mRNA stability occur between the two strains. 3. There is no evidence of a different number of glucokinase genes in the high- and low-activity strains. Differences in activity therefore suggest that dissimilarities in the regulation of the expression of these genes may well occur.
Middleton
R J
RJ
School of Biochemistry, University of Birmingham, Edgbaston, U.K.
Walker
D G
DG
D000818:Animals; D015139:Blotting, Southern; D003433:Crosses, Genetic; D015971:Gene Expression Regulation, Enzymologic; D005941:Glucokinase / Q000235:genetics* / Q000378:metabolism; D007328:Insulin / Q000097:blood; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000032:analysis
D007328:Insulin; D012333:RNA, Messenger; D005941:Glucokinase