Detection of intraspecific DNA polymorphism in Streptococcus salivarius subsp. thermophilus by a homologous rDNA probe.
Three ribosomal probes from Streptococcus salivarius subsp. thermophilus were cloned. Sequence data demonstrate that their juxtaposition corresponds to an entire operon. They were used in order to study ribosomal operon number and organization. rRNA genes were shown to be clustered in the order 5'-16S-23S-5S-3' and the number of rrn loci to vary within the subspecies. The smallest of the 3 probes was used for strain characterization. Substantial variability in hybridization patterns was observed among strains, resulting not only from, restriction fragment length polymorphism (RFLP) but also from the variability of ribosomal operon number.
Pébay
M
M
Laboratoire de Génétique et Microbiologie, Université de Nancy I, Faculté des Sciences, France.
Colmin
C
C
Guédon
G
G
De Gaspéri
C
C
Decaris
B
B
Simonet
J M
JM
D001345:Autoradiography; D001483:Base Sequence / Q000235:genetics; D003001:Cloning, Molecular; D015342:DNA Probes / Q000032:analysis*; D004275:DNA, Ribosomal / Q000235:genetics*; D006824:Hybridization, Genetic; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics*; D013291:Streptococcus / Q000145:classification / Q000235:genetics*; D012407:rRNA Operon / Q000235:genetics
D015342:DNA Probes; D004275:DNA, Ribosomal