title: [Diversity of antigenic fractions of allergenic extracts and importance of the Western blot technique].
abstract: The Phast-system groups together the techniques of electrophoresis and transfer. When applied to allergen extracts it allows identification of the allergenic protein fractions and shows their reactivity against sera from allergic patients. The Phast-system is indicated for the choice of the appropriate allergen for specific immunotherapy. If it is not possible to do it systematically, it should be done whenever there is a check to specific immunotherapy.
authors.affiliation: Laboratoire d'Immuno-Allergologie, C.H.U., Angers.
authors.forename: A
authors.initials: A
authors.lastname: Sabbah
authors.forename: M G
authors.initials: MG
authors.lastname: Lauret
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification*; D039741:Antigens, Dermatophagoides; D001514:Bee Venoms / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western / Q000295:instrumentation; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology; D008925:Mites / Q000737:chemistry / Q000276:immunology; D008970:Molecular Weight; D011058:Pollen / Q000737:chemistry / Q000276:immunology; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000485:Allergens; D000941:Antigens; D039741:Antigens, Dermatophagoides; D001514:Bee Venoms; D011506:Proteins
vernacular_title: Diversité des fractions antigéniques des extraits allergéniques et intérêt de la technique du Western blot.