title: The genome of the Mediterranean fruitfly Ceratitis capitata: localization of molecular markers by in situ hybridization to salivary gland polytene chromosomes.
abstract: We hybridized cloned DNA segments to salivary gland polytene chromosomes of the medfly, Ceratitis capitata, and thus established molecular markers for 24 sites on 6 out of 10 autosomal arms. An additional marker identified a medfly repetitive element that hybridizes to approximately 100 autosomal sites as well as a granular network that is thought to represent the X chromosome. Some of the markers correspond to 9 characterized transcription units, while 17 remain anonymous; at least 3 of the latter are restriction fragment length polymorphism (RFLP) markers. The characterized transcription units document that chromosomal arm 5L of C. capitata is homologous to the Drosophila melanogaster X chromosome, in agreement with previous inferences based on the extensive conservation of linkage groups in Diptera.
authors.affiliation: Department of Biology, University of Patras, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Zacharopoulou
authors.forename: M
authors.initials: M
authors.lastname: Frisardi
authors.forename: C
authors.initials: C
authors.lastname: Savakis
authors.forename: A S
authors.initials: AS
authors.lastname: Robinson
authors.forename: P
authors.initials: P
authors.lastname: Tolias
authors.forename: M
authors.initials: M
authors.lastname: Konsolaki
authors.forename: K
authors.initials: K
authors.lastname: Komitopoulou
authors.forename: F C
authors.initials: FC
authors.lastname: Kafatos
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA; D004175:Diptera / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics; D005819:Genetic Markers; D016678:Genome; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012469:Salivary Glands / Q000378:metabolism*; D014960:X Chromosome
chemical_list: D005819:Genetic Markers; D004247:DNA