[Characteristics and reactivities of a panel of monoclonal antibodies (McAbs) recognizing human colon cancer associated antigens].
Characteristics and reactivities of 16 monoclonal antibodies (McAbs) were identified. They were prepared from mice immunized with freshly isolated colon carcinoma cells. Of these 16 McAbs, 13 belonged to IgG1, and 3 to IgM. The reactivities by immunohistochemical staining revealed 2 varieties: tumor-associated antigens and common normal tissue antigens as defined by McAbs with 737 kinds of tissues and cells from more than 12,000 samples. The antigens defined by McAbs were assayed by western immunoblotting to have a about molecular weights of 200 KD as glycoproteins unrelated to CEA. These results suggest that these McAbs may be used for further research on cancers.
Liu
M P
MP
PLA General Hospital, Shenyang Command, Liaoning.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis; D003001:Cloning, Molecular; D003110:Colonic Neoplasms / Q000276:immunology* / Q000473:pathology; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C030139:colon-specific antigen