title: Mechanism of loss of thermodynamic control in mitochondria due to hyperthyroidism and temperature.
abstract: Incubation of normal mitochondria at 45 degrees C results in increases of respiration and of total apparent proton conductance (TAPC, respiration/proton motive force) and in an upward shift of the flow-force relationships. Similar effects are observed during operation of the redox proton pumps at different sites of the respiratory chain. These effects are accompanied by an almost equivalent increase of the passive proton conductance (PPC, proton leakage/proton motive force). In mitochondria from 3,3,5-triiodo-L-thyronine (T3)-treated rats there are also increases of respiration and of TAPC and an upward shift of flow-force relationships, more pronounced at the level of the cytochrome oxidase proton pump. However, at variance from the incubation at 45 degrees C, in mitochondria from T3-treated rats there is only a slight increase of PPC. Addition of bovine serum albumin to normal mitochondria incubated at 45 degrees C results in a marked depression of TAPC in the nonlinear range of the flow-force relationships. An equivalent effect is not observed in mitochondria from T3-treated rats. The experimental results have been compared with computer simulations obtained on the basis of a chemiosmotic model of energy transduction. The increase of TAPC following incubation at high temperature is apparently due to changes of the proton conductance mainly at the level of PPC, while the increase of TAPC following T3 administration is rather due to changes presumably at the level of the redox or ATPase proton pumps.
authors.affiliation: Consiglio Nazionale delle Ricerche Unit for the Study of the Physiology of Mitochondria, University of Padova, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Luvisetto
authors.forename: I
authors.initials: I
authors.lastname: Schmehl
authors.forename: E
authors.initials: E
authors.lastname: Intravaia
authors.forename: E
authors.initials: E
authors.lastname: Conti
authors.forename: G F
authors.initials: GF
authors.lastname: Azzone
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006980:Hyperthyroidism / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism / Q000502:physiology; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature; D013816:Thermodynamics; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D008565:Membrane Proteins; D014284:Triiodothyronine; D000255:Adenosine Triphosphate; D011188:Potassium