An ultrastructural study of hydrogen peroxide production by cultured fetal and neonatal rat lung cells exposed to hyperoxia.
Hydrogen peroxide production in organotypic cultures of fetal and newborn rat lung cells has been investigated using an ultrastructural histochemical method, in which the quantity and location of an electron-dense reaction product derived from the interaction of cerium chloride and hydrogen peroxide was detected. Hydrogen peroxide was present in fetal cell cultures exposed to hyperoxia (50% oxygen) compared to controls maintained in 10% oxygen. This increase could be limited by incubation of cultures with ascorbic acid and preincubation with dexamethasone. On the other hand, in newborn rat lung cell cultures, less hydrogen peroxide was identified in cultures including those exposed to hyperoxia (50% oxygen).
Loo
C K
CK
University of New South Wales, School of Pathology, Carcinogenesis Research Unit, Kensington, Australia.
Smith
G J
GJ
Lykke
A W
AW
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002563:Cerium / Q000032:analysis; D005260:Female; D005333:Fetus; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008168:Lung / Q000187:drug effects / Q000196:embryology / Q000378:metabolism / Q000648:ultrastructure*; D010100:Oxygen / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
D002563:Cerium; D006861:Hydrogen Peroxide; D010100:Oxygen