Detection of borreliacidal antibodies in hamsters by using flow cytometry.
Flow cytometry can be used to detect antibody that kills Borrelia burgdorferi. Borreliacidal activity was detected within 3 h of incubating B. burgdorferi with immune serum and complement. Right-angle light scatter and propidium iodide fluorescence were the cytometric parameters which correlated best with in vitro killing of B. burgdorferi. Flow cytometry is a rapid method for determining the presence of borreliacidal activity and may lead to a better serodiagnostic test for the detection of Lyme disease.
Sachsenmeier
K F
KF
Wisconsin State Laboratory of Hygiene, Department of Medical Microbiology and Immunology, University of Wisconsin, Madison 53706.
Schell
K
K
Morrissey
L W
LW
Pennell
D R
DR
West
R M
RM
Callister
S M
SM
Schell
R F
RF
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D015748:Borrelia burgdorferi Group / Q000276:immunology* / Q000302:isolation & purification; D006224:Cricetinae; D005434:Flow Cytometry / Q000379:methods*
D000907:Antibodies, Bacterial