SARG: a new human osteosarcoma cell line. Expression of bone markers and of major histocompatibility antigens.
A new cell line (SARG) was established from a human radiation-induced osteosarcoma (OSA). It showed an epithelial-like morphology with polymorphous and sometimes bizarre nuclei. SARG had an osteoblastic differentiation pattern: almost 100% of the cells were positive for alkaline phosphatase, type I and III collagens and osteonectin. The expression of class I HLA antigens was detectable even after 40 in vitro passages. The expression of MHC antigens was greatly increased after in vitro treatment with interferon gamma (IFN-gamma), whereas interferon alpha (IFN-alpha) and tumor necrosis factor alpha (TNF-alpha) increased the expression of class I antigens, but not of class II antigens. SARG was tumorigenic after subcutaneous injection in nude mice. Experimental metastases were never detected.
Scotlandi
K
K
Laboratorio di Ricerca Oncologica, Istituto Ortopedico Rizzoli, Bologna, Italy.
Serra
M
M
Landuzzi
L
L
Baldini
N
N
D000328:Adult; D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D001859:Bone Neoplasms / Q000737:chemistry* / Q000276:immunology; D016207:Cytokines / Q000494:pharmacology; D006680:HLA Antigens / Q000032:analysis; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009381:Neoplasms, Radiation-Induced / Q000737:chemistry* / Q000276:immunology; D012516:Osteosarcoma / Q000737:chemistry* / Q000276:immunology; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D014408:Biomarkers, Tumor; D016207:Cytokines; D006680:HLA Antigens; D006649:Histocompatibility Antigens