Mechanisms of oligodendrocyte interaction with normal human serum--defining the role of complement.
The interaction of human serum with oligodendroglia was investigated in vitro using purified cultured neonatal rat oligodendrocytes. Previous evidence for antibody independent classical pathway complement activation was confirmed; the results also showed a deficit in the protection of rat oligodendrocytes from complement attack suggesting a deficiency in the expression of terminal regulatory proteins of the complement cascade. Thus, rat oligodendrocytes are selectively sensitive to normal serum due both to complement activation and impaired protection from terminal complement attack.
Zajicek
J
J
University of Cambridge Neurology Unit, Addenbrookes Hospital, U.K.
Wing
M G
MG
Lachmann
P J
PJ
Compston
D A
DA
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000502:physiology; D001790:Blood Physiological Phenomena; D001812:Blood-Brain Barrier; D002478:Cells, Cultured; D003172:Complement C1 / Q000494:pharmacology; D003176:Complement C3 / Q000494:pharmacology; D015938:Complement Membrane Attack Complex / Q000502:physiology*; D003171:Complement Pathway, Classical; D003711:Demyelinating Diseases; D009836:Oligodendroglia / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D003172:Complement C1; D003176:Complement C3; D015938:Complement Membrane Attack Complex