Involvement of carbonic anhydrase in ammonia flux across rumen mucosa in vitro.
Carbonic anhydrase activity in mucosa of the sheep rumen wall was completely inhibited by acetazolamide and ethoxyzolamide. This inhibition significantly reduced ammonia flux across mucosal discs in vitro but was ineffective if short-chain fatty acids were present in the bathing solution. The findings are discussed in relation to the mechanism of ruminal ammonia absorption.
Bödeker
D
D
Department of Physiology, School of Veterinary Medicine, Hannover, Federal Republic of Germany.
Oppelland
G
G
Höller
H
H
D000086:Acetazolamide / Q000494:pharmacology; D000641:Ammonia / Q000378:metabolism / Q000493:pharmacokinetics*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002256:Carbonic Anhydrases / Q000032:analysis / Q000502:physiology*; D005016:Ethoxzolamide / Q000494:pharmacology; D005232:Fatty Acids, Volatile / Q000502:physiology; D005753:Gastric Mucosa / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D012417:Rumen / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D012756:Sheep
D005232:Fatty Acids, Volatile; D000641:Ammonia; D002256:Carbonic Anhydrases; D000086:Acetazolamide; D005016:Ethoxzolamide