Effective detergent/lipid ratios in the solubilization of phosphatidylcholine vesicles by Triton X-100.
Effective detergent:lipid ratios (i.e. molar ratios in the mixed aggregates, vesicles or micelles) have been estimated for the solubilization of phosphatidylcholine vesicles by Triton X-100. Effective molar ratios are given for both the onset and the completion of bilayer solubilization; small unilamellar, large unilamellar and multilamellar vesicles have been used. Effective detergent:lipid ratios are independent of phospholipid concentration, and their use allows a deeper understanding of membrane-surfactant interactions.
Partearroyo
M A
MA
Department of Biochemistry, University of the Basque Country, Bilbao, Spain.
Urbaneja
M A
MA
Goñi
F M
FM
D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D017830:Octoxynol; D010713:Phosphatidylcholines / Q000032:analysis* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000032:analysis* / Q000494:pharmacology; D012995:Solubility
D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols; D017830:Octoxynol