Comparison of locations and peptide content of postganglionic neurons innervating veins and arteries of the rat hindlimb.
The ganglionic location of hindlimb vasoconstrictor sympathetic neurons in several species is known but the locations of neurons innervating limb arteries or veins, specifically, have not been compared and neurochemical differences between them have not been examined in detail. This study was designed to determine whether neurons innervating arteries and veins are organized as distinct populations and whether neurons innervating arteries, veins or footpads contain the same peptides. Retrograde transport of fluorescent dyes was used to identify, separately, paravertebral postganglionic neurons in the 13th thoracic to 6th lumbar (T13-L6) chain ganglia that innervate the femoral arteries, femoral veins and footpads of the rat hindlimb. The proportions of venous and arterial vasomotor neurons and footpad neurons containing neuropeptide Y- and vasoactive intestinal polypeptide-immunoreactivity (NPY-Ir, VIP-Ir) were then compared. Venous vasomotor neurons were found mostly (62%) in the L1 and L2 ganglia. The majority of arterial vasomotor neurons (81%) were distributed slightly more caudally in L1-L3. Veins and arteries were not innervated by the same cells. Footpad neurons were located mostly in L4-L6. NPY-Ir was identified in 17% of the venomotor neurons, in 94% of arterial neurons and in 24% of footpad neurons. VIP-Ir was found in 3% of the venomotor neurons, 8% of the arterial neurons and in 44% of the footpad neurons. In conclusion, hindlimb venous and arterial vasomotor neurons are anatomically distinct, are mixed randomly within the chain ganglia and differ markedly in their content of NPY, consistent with reported differences in neuromuscular transmission to arteries and veins. The most likely hindlimb postganglionic neurons to contain VIP were those innervating footpads, probably controlling sweat gland function.
Dehal
N S
NS
John P. Robarts Research Institute, London, Ontario, Canada.
Kartseva
A
A
Weaver
L C
LC
D000818:Animals; D001158:Arteries / Q000294:innervation / Q000378:metabolism; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000378:metabolism; D006614:Hindlimb / Q000098:blood supply / Q000294:innervation*; D006651:Histocytochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow / Q000502:physiology; D013545:Sweat Glands / Q000294:innervation / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism; D014680:Veins / Q000294:innervation / Q000378:metabolism
D009478:Neuropeptide Y; D009479:Neuropeptides; D014660:Vasoactive Intestinal Peptide