Loading and transfer control for Northern hybridization.
We report a simple and inexpensive method to quantitate loading and transfer of RNA and to examine RNA integrity for use with Northern hybridization. This technique involves quantitation by computer-assisted video densitometry of a negative photograph of 28S and 18S rRNA from ethidium bromide-stained RNA fixed to nylon membranes. This method eliminates the issue of variability of expression of "housekeeping" genes and the need for a second round of hybridization to quantitate control probes.
Correa-Rotter
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Department of Medicine, University of Minnesota, Minneapolis 55455.
Mariash
C N
CN
Rosenberg
M E
ME
D000818:Animals; D015152:Blotting, Northern / Q000295:instrumentation / Q000379:methods*; D003626:Data Display; D003720:Densitometry / Q000295:instrumentation; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008838:Microcomputers; D012337:RNA, Ribosomal, 18S / Q000032:analysis*; D012339:RNA, Ribosomal, 28S / Q000032:analysis*; D051381:Rats; D012984:Software; D014741:Video Recording
D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S