Direct determination of the enantiomeric ratio of verapamil, its major metabolite norverapamil and gallopamil in plasma by chiral high-performance liquid chromatography.
Two methods for the determination of the enantiomeric ratio of verapamil in plasma by high-performance liquid chromatography have been developed. On an alpha 1-acid glycoprotein chiral stationary phase (Chiral-AGP) verapamil was separated after the acetylation of the main metabolite norverapamil, which interferes with the resolution of verapamil. On an amylose tris-3,5-dimethylphenylcarbamate column (Chiralpak AD) verapamil and norverapamil were determined simultaneously without prior derivatization. Gallopamil was separated on both columns under similar conditions.
Fieger
H
H
Institute of Pharmaceutical Chemistry, University of Münster, Germany.
Blaschke
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005711:Gallopamil / Q000032:analysis / Q000097:blood*; D006801:Humans; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D013237:Stereoisomerism; D014700:Verapamil / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood*
D005711:Gallopamil; C016904:norverapamil; D014700:Verapamil