title: A low-spin iron complex in human melanoma and rat hepatoma cells and a high-spin iron(II) complex in rat hepatoma cells.
abstract: Human melanoma and rat hepatoma cells cultured in the presence of low concentrations (2.5 microM) of low-molecular-weight iron (Fe) chelates and Fe-transferrin complexes have been studied with 57Fe Mössbauer spectroscopy. The spectra show that holoferritin is only a minor fraction of the total iron present in the cells. The major form of Fe was in a low-spin state unlike the high-spin Fe(III) found in ferritin. Only about 10% of the Fe could be attributed to ferritin. In addition, the hepatoma cells had a high-spin Fe(II) spectral component which made up about 20% of the Fe present.
authors.affiliation: School of Mathematical and Physical Sciences, Murdoch University, Perth, Australia.
authors.forename: T G
authors.initials: TG
authors.lastname: St Pierre
authors.forename: D R
authors.initials: DR
authors.lastname: Richardson
authors.forename: E
authors.initials: E
authors.lastname: Baker
authors.forename: J
authors.initials: J
authors.lastname: Webb
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology*; D005293:Ferritins / Q000032:analysis; D006801:Humans; D007502:Iron Chelating Agents / Q000494:pharmacology*; D033862:Iron-Binding Proteins; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D015204:Spectroscopy, Mossbauer; D033863:Transferrin-Binding Proteins; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D002352:Carrier Proteins; D007502:Iron Chelating Agents; D033862:Iron-Binding Proteins; D033863:Transferrin-Binding Proteins; D005293:Ferritins