title: PEA1 and PEA3 enhancer elements are primary components of the polyomavirus late transcription initiator element.
abstract: The circular polyomavirus genome is transcribed from divergent promoter regions. Early mRNAs are initiated from a transcription complex formed at a TATA motif, the site of binding of transcription factor TFIID. Early transcription is promoted at a distance by the viral enhancer, which includes DNA motifs bound by cellular proteins of the PEA1 and PEA3 families of transcription activators. In contrast, the predominant viral late mRNAs are initiated within the viral enhancer, which lacks a TATA motif, near the PEA1 and PEA3 DNA motifs. Here, we demonstrate that these PEA1 and PEA3 binding sites are primary components of an autonomous transcription initiator element (Inr). They cause transcription of most polyomavirus late mRNAs and can direct the transcription of heterologous reporter genes. Alternative roles of these DNA motifs as activators of early mRNA transcription and as an initiator element for late mRNA transcription help explain how polyomavirus gene expression is regulated during lytic growth and provides a model for cellular transcription during development.
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia 65212.
authors.forename: W
authors.initials: W
authors.lastname: Yoo
authors.forename: M E
authors.initials: ME
authors.lastname: Martin
authors.forename: W R
authors.initials: WR
authors.lastname: Folk
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005814:Genes, Viral; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D000952:Antigens, Polyomavirus Transforming; D050659:Core Binding Factor Alpha 1 Subunit; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; C493716:Runx2 protein, mouse; D050656:Transcription Factor AP-2; D014157:Transcription Factors; C060759:enhancer-binding protein AP-3