Vasoactive intestinal peptide receptors on AR42J rat pancreatic acinar cells.
VIP receptors on AR42J rat pancreatic cells were analyzed by competition binding, affinity labeling and by N-glycanase digestion analyses. These studies revealed the presence of specific, high affinity (Kd approximately 1 nM) VIP receptors with a mass of 67 kDa or 59 kDa under reducing or non-reducing conditions, respectively. N-glycanase digestion of affinity labeled membranes generated a core receptor protein of approximately 44 kDa and evidence for at least two N-linked glycans on the mature receptor. The receptor lacked O-linked oligosaccharides but contained terminal sialic acid residues on its N-linked glycan(s) based on digestions with O-glycanase and neuraminidase. The similarity of the AR42J VIP receptor to the recently cloned cDNA for human VIP receptors makes this cell line an attractive model for further analysis of VIP receptor signal transduction events.
Raymond
M J
MJ
Department of Ophthalmology & Visual Science, Yale University School of Medicine, New Haven, CT 06510.
Rosenzweig
S A
SA
D000818:Animals; D001667:Binding, Competitive; D002241:Carbohydrates / Q000032:analysis; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity; D005934:Glucagon / Q000494:pharmacology; D006026:Glycoside Hydrolases; D007700:Kinetics; D008970:Molecular Weight; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011964:Receptors, Gastrointestinal Hormone / Q000302:isolation & purification / Q000378:metabolism*; D018005:Receptors, Vasoactive Intestinal Peptide; D012633:Secretin / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000302:isolation & purification / Q000378:metabolism*
D002241:Carbohydrates; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D012633:Secretin; D014660:Vasoactive Intestinal Peptide; D005934:Glucagon; D006026:Glycoside Hydrolases