PCR-generated conspecific sodium channel gene probe for the house fly.
A segment of the house fly (Musca domestica) homologue of the para (paralytic) sodium channel gene of Drosophila melanogaster was isolated by using mixed sequence oligonucleotide primers in the polymerase chain reaction (PCR). The specificity of the procedure was demonstrated by genomic Southern analysis using the housefly PCR amplification product as a probe and by DNA sequence analysis. The latter showed structural homology to the para gene, but not to the corresponding region of DSC1, another D. melanogaster gene with structural similarity to vertebrate sodium channel genes.
Knipple
D C
DC
Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva 14456.
Payne
L L
LL
Soderlund
D M
DM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004330:Drosophila / Q000235:genetics; D006793:Houseflies / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D015222:Sodium Channels / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D015222:Sodium Channels; D004247:DNA