title: Physical mapping of the mec region of an American methicillin-resistant Staphylococcus aureus strain.
abstract: We mapped part of the mec region of a locally prevalent strain of Staphylococcus aureus. The mec region was found to harbor an insert of the transposon Tn554, which encodes spectinomycin and macrolide-lincosamide-streptogramin B resistance, and a 4.6-kb segment of DNA that contains the kanamycin resistance gene aadD. This 4.6-kb segment appears to be an integrated form of a previously described plasmid, pUB110, and is flanked by copies of the insertion sequence IS257. The integration event may be an example of processes that have led to accretion of resistance determinants in the mec region of S. aureus.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854-5635.
authors.forename: D T
authors.initials: DT
authors.lastname: Dubin
authors.forename: P R
authors.initials: PR
authors.lastname: Matthews
authors.forename: S G
authors.initials: SG
authors.lastname: Chikramane
authors.forename: P R
authors.initials: PR
authors.lastname: Stewart
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D007613:Kanamycin Resistance / Q000235:genetics; D016106:Methicillin Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial