Unusual cell specific expression of a major human cytomegalovirus immediate early gene promoter-lacZ hybrid gene in transgenic mouse embryos.
Transgenic mice carrying the human cytomegalovirus immediate early gene promoter driving the E. coli lacZ gene displayed an unusual cell specific expression of beta-galactosidase during development. LacZ expression was first detected in cells lining the apex of the neural fold of day 8.5 embryos. By day 10 of gestation, expression was prominent in the spinal ganglia, the ganglia of cranial nerves V, VII, VIII, IX, and X, in a line of cells marking the ventrolateral pathway adjacent to the dermamyotome, and in a column of differentiated cells in the entire ventrolateral neural tube posterior to the mesencephalon. Expression was also found in the myotomes. Neural tube explants from day 8.5 embryos cultured in vitro showed lacZ expression in cells migrating away from the explant. We conclude that the HCMV-IEP-lacZ transgene is expressed in a subpopulation of neural crest cells and its early derivatives.
Kothary
R
R
Department of Molecular Embryology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
Barton
S C
SC
Franz
T
T
Norris
M L
ML
Hettle
S
S
Surani
M A
MA
D000818:Animals; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004622:Embryo, Mammalian / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009432:Neural Crest / Q000166:cytology / Q000196:embryology; D011247:Pregnancy; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D014760:Viral Fusion Proteins; D004247:DNA; D001616:beta-Galactosidase