Phosphoglucoisomerase-catalyzed interconversion of hexose phosphates: distinction of the 1-monodeutero-isotopomers of D-fructose 6-phosphate by 1H NMR spectroscopy.
The 1H NMR spectrum obtained with the alpha- and beta-anomers of D-[1-2H]fructose 6-phosphate generated from D-glucose 6-phosphate sequentially exposed in D2O to phosphoglucoisomerase, phosphofructokinase and fructose-1,6-diphosphatase differed from that recorded when the deuterated ketohexose phosphate was produced from D-mannose 6-phosphate sequentially exposed in D2O to phosphomannoisomerase, phosphofructokinase and fructose-1,6-diphosphatase. The identification of the 2 isotopomers of D-fructose 6-phosphate by 1H NMR spectroscopy provides a new tool to assess the relative extent of interconversion of hexose phosphates in the reactions catalyzed by phosphoglucoisomerase and phosphomannoisomerase, respectively.
Willem
R
R
Hoog Resolutie NMR-Centrum (HR-NMRC) en Algemene en Organische Scheikunde (AOSC-TW), Vrije Universiteit Brussel, Belgium.
Malaisse-Lagae
F
F
Malaisse
W J
WJ
D003903:Deuterium; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005636:Fructosephosphates / Q000737:chemistry* / Q000378:metabolism; D019298:Glucose-6-Phosphate; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism*; D005958:Glucosephosphates / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008359:Mannose-6-Phosphate Isomerase / Q000378:metabolism; D008360:Mannosephosphates / Q000378:metabolism; D025481:Phosphofructokinase-2; D010770:Phosphotransferases / Q000378:metabolism
D005636:Fructosephosphates; D005958:Glucosephosphates; D008360:Mannosephosphates; C027693:mannose-6-phosphate; D019298:Glucose-6-Phosphate; C027618:fructose-6-phosphate; D003903:Deuterium; D010770:Phosphotransferases; D025481:Phosphofructokinase-2; D006597:Fructose-Bisphosphatase; D008359:Mannose-6-Phosphate Isomerase; D005956:Glucose-6-Phosphate Isomerase