Activation of Epstein-Barr virus promoters by a growth-factor and a glucocorticoid.
Transforming growth factor-beta (TGF-beta) and a glucocorticosteroid, Dexamethasone (DXM), both cause transcriptional induction of Epstein-Barr virus (EBV) early antigens (EA) in Daudi lymphoma cells. The viral induction occurs through the viral promoter DR overlapping an origin of replication which is active during the lytic cycle. Each hormone requires specific regions on the DR promoter. Since these regions also mediate the action of two viral transcription factors, EB1 and R, it may be emphasized that EB1 and/or R are involved in the EA induction process by TGF-beta and by DXM.
Schuster
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Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
Chasserot-Golaz
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Beck
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D000956:Antigens, Viral / Q000235:genetics*; D003907:Dexamethasone / Q000494:pharmacology*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000276:immunology; D008223:Lymphoma; D015735:Mifepristone / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000956:Antigens, Viral; C033305:Epstein-Barr virus early antigen; D016212:Transforming Growth Factor beta; D015735:Mifepristone; D003907:Dexamethasone