title: Molecular epidemiology of a coxsackievirus B3 outbreak.
abstract: An outbreak of coxsackievirus B3 infection occurred in South Africa in 1984 with a variety of clinical manifestations being observed. Fifty-one isolates from patients ranging in age from young babies to middle-aged adults were obtained. To define further the epidemiology of this outbreak all isolates were characterised by either 1- or 2-dimensional oligonucleotide mapping. One-dimensional mapping was found to be highly successful for initial screening of the isolates before further characterisation by 2-dimensional fingerprinting. All isolates were found to be essentially the same strain of coxsackievirus B3 although slight variations in both the 1- and 2-dimensional patterns could be observed. Some coxsackievirus B3 strains from geographically unrelated regions but isolated during the same time period as the outbreak showed clearly distinguishable oligonucleotide maps.
authors.affiliation: Department of Virology, University of the Witwatersrand, Republic of South Africa.
authors.forename: E
authors.initials: E
authors.lastname: Rossouw
authors.forename: C W
authors.initials: CW
authors.lastname: Tsilimigras
authors.forename: B D
authors.initials: BD
authors.lastname: Schoub
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D002675:Child, Preschool; D003384:Coxsackievirus Infections / Q000175:diagnosis* / Q000453:epidemiology; D004196:Disease Outbreaks; D029822:Enterovirus B, Human / Q000235:genetics*; D006801:Humans; D007223:Infant; D009710:Nucleotide Mapping / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry; D013019:South Africa / Q000453:epidemiology
chemical_list: D009841:Oligonucleotides