[Isolation and study of cAMP-independent chromatin protein kinases from brain cells].
Two cAMP-independent protein kinases were purified from rat brain neuron chromatin by using extraction with ammonium sulfate with subsequent chromatography on DEAE-Sephadex A-25 and Sephadex G-150. These enzymes were identified as casein kinases NI and NII, respectively. The molecular masses of the proteins as determined by gel filtration are 4500 and 130 Da. Casein kinase NII utilizes ATP (Km = 7.5 mM) and GTP (Km = 8.5 mM) as substrates, while casein kinase NI utilizes only ATP (Km = 6 mM). The activities of the both enzymes are inhibited by Mn2+ and Ca2+, while heparin (1 microgram/ml) inhibits only casein kinase NII. The memory stimulator ethymizol (ethylnorantipheine) increases the activity of casein kinase NII only when brain proteins extracted by 0.35 M NaCl or rat liver HMG-proteins are used as reaction substrates. This substance has no effect on the phosphorylation of casein and histone HI. The role of casein kinase NII of neuronal chromatin in the realization of stimulatory effects of physiologically active substances on RNA synthesis is discussed.
Bogdanova
N A
NA
Kulikova
O G
OG
D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D047388:Casein Kinases; D002843:Chromatin / Q000378:metabolism*; D002850:Chromatography, Gel; D000242:Cyclic AMP / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006609:High Mobility Group Proteins / Q000302:isolation & purification / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D009474:Neurons / Q000201:enzymology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats
D002843:Chromatin; D006609:High Mobility Group Proteins; D000242:Cyclic AMP; D011494:Protein Kinases; D047388:Casein Kinases
Vydelenie i issledovanie cAMP-nezavisimykh proteinkinaz khromatina iz kletok golovnogo mozga.