Genomic structure and expression of human guanosine monophosphate reductase.
In vitro translation in the rabbit reticulocyte system and transient expression in Cos7 cells were performed to characterize the protein encoded by a chromosome 6-linked human cDNA clone, whose nucleotide sequence is homologous to that of Escherichia coli guanosine monophosphate reductase (GMP reductase) cDNA. The molecular weight of the peptide produced by the cDNA was about 37,000 Dalton, and the protein produced in the Cos7 cells exhibited GMP reductase activity, substantiating that the cDNA is for human GMP reductase. The corresponding genomic clones were obtained from two human genomic libraries. The gene spans about 50 kb and is composed of 9 exons, which encode 345 amino acid residues. Organization of exons and introns was established by DNA sequencing of each exon and splicing junctions. The gene contains two potential SpI binding sites within exon 1, and a functional atypical polyadenylation signal in exon 9.
Kondoh
T
T
Department of Biochemical Genetics, Beckman Research Institute of the City of Hope, Duarte, CA 91010.
Kanno
H
H
Chang
L
L
Yoshida
A
A
D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D050899:GMP Reductase; D015870:Gene Expression; D006157:Guanosine Monophosphate / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D006157:Guanosine Monophosphate; D009247:NADH, NADPH Oxidoreductases; D050899:GMP Reductase