Use of immobilized triazine dyes in the purification of DNA topoisomerase I (Topo I) and terminal deoxynucleotidyl transferase (TdT) from calf thymus.
The interaction between TdT and Topo I, and twelve various triazine dyes immobilized on Sepharose CL-6B was studied. Yellow lightproof 2KT-Sepharose and Bordeaux 4ST-Sepharose were used to purify TdT and Topo I, respectively. The principal role of copper ions, complexed to the dye molecules, in the dye-protein interaction was evaluated.
Markauskas
A
A
Institute of Applied Enzymology FERMENTAS, Lithuania, U.S.S.R.
Tiknius
V
V
Marcisauskas
R
R
D000818:Animals; D002417:Cattle; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D003300:Copper; D004253:DNA Nucleotidylexotransferase / Q000302:isolation & purification*; D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification*; D013950:Thymus Gland / Q000737:chemistry* / Q000201:enzymology; D014227:Triazines
D014227:Triazines; D003300:Copper; D004253:DNA Nucleotidylexotransferase; D004264:DNA Topoisomerases, Type I