Localization of [3H]GABA-labelled nerve fibre networks in the rat intestinal mucosa.
Laminar preparations of the rat colonic mucosa were treated for [3H]GABA (5 x 10(-8) M) autoradiography. Under conditions specific for high-affinity labelling of neuronal elements, a network of fibres overlying the base of the mucosal crypts and a second network coursing in close association with the crypts, were intensely labelled. Along the course of the overlying network of fibres, were dense accumulations of silver grains reminiscent of junctions for fibres projecting into the mucosa. All labelling could be prevented by the specific neuronal uptake inhibitor, L-DABA (10(-3) M).
Krantis
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A
Dept. of Physiology, University of Ottawa, Ontario, Canada.
Clark
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D000613:Aminobutyrates / Q000494:pharmacology; D000625:Aminooxyacetic Acid / Q000494:pharmacology; D000818:Animals; D003106:Colon / Q000294:innervation; D007413:Intestinal Mucosa / Q000294:innervation*; D008297:Male; D009412:Nerve Fibers / Q000737:chemistry* / Q000648:ultrastructure; D009434:Neural Pathways / Q000737:chemistry / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000032:analysis*; D015091:beta-Alanine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D000613:Aminobutyrates; D011963:Receptors, GABA-A; D015091:beta-Alanine; D000625:Aminooxyacetic Acid; C005959:2,4-diaminobutyric acid; D005680:gamma-Aminobutyric Acid