Nerve growth factor enhances DNA synthesis in cultured cerebellar neuroblasts.
The cerebellar neuroblasts in primary cultures from five-day-old rats bore NGF receptor immunoreactivity, suggesting a potential responsive to NGF. At low plating density, NGF was found to enhance DNA synthesis in these cells in a dose-dependent manner. As these cells synthesize NGF, one possibility to account for the lack of response of neuroblasts plated at high density is that the amount of endogenous trophic agent produced in this culture condition is sufficient to ensure an optimal effect. The results demonstrate that premitotic neuroblasts in the CNS, as well postmitotic neurons, are responsive to NGF. At the early stage of its development, the cerebellum therefore appears to be a very good autocrine model of NGF action.
Confort
C
C
Laboratoire de Neurobiologie Endocrinologie, URA 1197 du CNRS, Université Montpellier II, France.
Charrasse
S
S
Clos
J
J
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects*; D007150:Immunohistochemistry; D007700:Kinetics; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis / Q000187:drug effects / Q000502:physiology; D017475:Receptors, Nerve Growth Factor; D013936:Thymidine / Q000378:metabolism
D009414:Nerve Growth Factors; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D004247:DNA; D013936:Thymidine