title: Alpha-hemolysin contributes to the pathogenicity of piliated digalactoside-binding Escherichia coli in the kidney: efficacy of an alpha-hemolysin vaccine in preventing renal injury in the BALB/c mouse model of pyelonephritis.
abstract: Digalactoside-binding (Gal-Gal) pili and alpha-hemolysin of Escherichia coli have been implicated as important virulence determinants in the pathogenesis of human ascending, nonobstructive pyelonephritis. The pathogenic significance of these determinants was evaluated in vitro and in the BALB/c mouse pyelonephritis model by employing wild-type, avirulent laboratory, and genetically defined cosmids, transformants, and recombinant strains. In vitro data suggest that the cytolytic activity of hemolysin is significantly (P less than 0.05) enhanced among digalactoside-binding strains which agglutinate erythrocytes. The basis of increased hemolysis is related presumably to more efficient delivery of the toxin to target lipid substrate in the host plasma membrane. Intravesicular administration of bacteria that express both digalactoside binding and hemolysin generally resulted in greater mortality and renal parenchymal injury in mice than strains that expressed none or only one of these determinants. Analyses convincingly demonstrate that digalactoside-binding pili are correlated with upper urinary tract colonization and that hemolysin is correlated with septicemia and renal parenchymal damage. These determinants collectively constitute the minimal virulence factors to produce disease in this model. Their efficacy as vaccines for the prevention of pyelonephritis was also assessed. A purified Gal-Gal pilus vaccine prevented (P less than 0.05) subsequent colonization by a challenge wild-type strain that exhibited homologous pili. The hemolysin vaccine did not abrogate subsequent bacterial renal colonization on challenge, but it did protect (P less than 0.05) mice which survived challenge from subsequent renal injury compared with those in the saline control group. The combination of these determinants was also protective. The combination of Gal-Gal pili and hemolysin in a vaccine preparation represents a potentially worthwhile strategy for human immunoprophylaxis against pyelonephritis by interdicting several steps in the pathogenesis of a bacterial mucosal infection.
authors.affiliation: Department of Medicine, Stanford University, California 94305.
authors.forename: P
authors.initials: P
authors.lastname: O'Hanley
authors.forename: G
authors.initials: G
authors.lastname: Lalonde
authors.forename: G
authors.initials: G
authors.lastname: Ji
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology / Q000633:toxicity*; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D002240:Carbohydrate Sequence; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D005260:Female; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D005697:Galactosides / Q000378:metabolism*; D006385:Hemagglutination Inhibition Tests; D006460:Hemolysin Proteins; D007114:Immunization; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D007841:Latex Fixation Tests; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011704:Pyelonephritis / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D029968:Escherichia coli Proteins; D005697:Galactosides; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli