[Diagnosis of cytomegalovirus infections in hospitalized patients].
Rapid detection of cytomegalovirus (CMV) infections, especially in immunocompromised patients, is important and sometimes lifesaving. We describe one year's experience of using a combination of several methods: detection of "immediate early" CMV antigen in blood, detection of "early" virus antigen after brief incubation in cell culture, detection of viral DNA by polymerase chain reaction, regular culture in human embryo fibroblasts, and serological detection of IgM and IgG antibodies. A quick and early diagnosis was achieved by all three rapid methods. However, none of the methods is sufficiently sensitive or specific to allow it to be used alone. A combination of several methods is recommended in order to achieve maximum efficiency and safety.
Degré
M
M
Kaptein W. Wilhelmsen og frues bakteriologiske institutt, Rikshospitalet, Oslo.
Rollag
H
H
Holter
E
E
Tønjum
T
T
Krajci
Z
Z
Bukholm
G
G
D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D005069:Evaluation Studies as Topic; D006760:Hospitalization; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007153:Immunologic Deficiency Syndromes / Q000150:complications / Q000276:immunology; D007158:Immunologic Techniques; D008828:Microbiological Techniques; D009894:Opportunistic Infections / Q000276:immunology; D016133:Polymerase Chain Reaction; D012698:Serologic Tests / Q000379:methods
D007074:Immunoglobulin G; D007075:Immunoglobulin M
Diagnostikk av cytomegalovirusinfeksjoner hos sykehuspasienter.