title: An improved Tn7-based system for the single-copy insertion of cloned genes into chromosomes of gram-negative bacteria.
abstract: A system is described for the single-copy, stable insertion of cloned DNA sequences into the chromosomes of Gram- bacteria. Two narrow-host-range plasmids form the basis of this system: the 'carrier' plasmid contains the mini Tn7-Km transposon, into which foreign DNA can be cloned; the 'helper' plasmid provides the Tn7 transposition functions in trans. Both plasmids are readily transferred into Gram- bacteria by conjugation. The functionality of this system has been demonstrated in Rhodospirillum rubrum.
authors.affiliation: Department of Bacteriology, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706.
authors.forename: Y
authors.initials: Y
authors.lastname: Bao
authors.forename: D P
authors.initials: DP
authors.lastname: Lies
authors.forename: H
authors.initials: H
authors.lastname: Fu
authors.forename: G P
authors.initials: GP
authors.lastname: Roberts
mesh_terms: D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012247:Rhodospirillum rubrum / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements