title: Protein recovery from the all-agarose ProSieve gel system.
abstract: Electrophoresis in polyacrylamide gels is one of the most powerful tools used for the analysis of proteins. However, this technique is not widely used for protein purification for a variety of reasons such as the following: less than quantitative recoveries; involved, time-consuming methodologies; and impurities in the protein preparations from gel-polymerization by-products that can modify the proteins and interfere with subsequent experiments. As an alternative, we have developed a simple and quantitative recovery procedure for proteins separated by electrophoresis in the all-agarose ProSieve gel system. Using this procedure, greater than 90% of each protein examined was recovered, and these proteins were unaffected by the recovery procedure.
authors.forename: J H
authors.initials: JH
authors.lastname: Morgan
authors.forename: F P
authors.initials: FP
authors.lastname: Curtis
authors.forename: S
authors.initials: S
authors.lastname: Nochumson
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification; D002256:Carbonic Anhydrases / Q000302:isolation & purification; D002417:Cattle; D003574:Cytochrome c Group / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004926:Escherichia coli; D008970:Molecular Weight; D009218:Myosins / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012394:Rosaniline Dyes; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D003574:Cytochrome c Group; D011506:Proteins; D012394:Rosaniline Dyes; D012710:Serum Albumin, Bovine; C048139:Coomassie blue; D009218:Myosins; D002256:Carbonic Anhydrases