Correlation between gene expression and chromatin conformation of c-fos and N-ras in human liver and hepatoma.
In situ hybridization revealed an apparently higher expression of N-ras and c-fos in human hepatoma than in its adjacent liver tissue. When the isolated nuclei were partially digested with micrococcal nuclease followed by DNA extraction, gel electrophoresis and hybridization with labelled probes, it was shown that in hepatoma the c-fos and N-ras DNA sequences were mainly distributed on the nucleosomes of lower order structures, while these gene sequences in the adjacent liver tissue were found mainly on the nucleosomes of higher order structures. These results clearly indicate a close correlation between gene expression and chromatin conformation of c-fos and N-ras.
Wang
Z
Z
Institute of Basic Medical Sciences, CAMS, Beijing.
Xiang
Q
Q
Li
D
D
Li
S
S
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002843:Chromatin / Q000737:chemistry; D004273:DNA, Neoplasm; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*
D002843:Chromatin; D004273:DNA, Neoplasm; D009707:Nucleosomes; D016760:Proto-Oncogene Proteins c-fos