title: A one-minute oxidase test to detect Vibrio strains isolated from cultures on thiosulphate-citrate-bile salts-sucrose (TCBS) medium.
abstract: Vibrio cholerae is oxidase positive, a primary characteristic used to differentiate it from Enterobacteriaceae. But false negative oxidase test results have been obtained with colonies from thiosulphate-citrate-bile salts-sucrose (TCBS) agar medium. A rapid oxidase test procedure is described here. This takes 1 min, avoids false negative results and the necessity to grow the bacteria in a general-purpose medium. The bacteria may be recovered after the test and used for further investigations.
authors.affiliation: Servei de Microbiologia i Parasitologia, Hospital Clinic i Provincial de Barcelona, Facultat de Medicina, Universitat de Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Vila
authors.forename: S
authors.initials: S
authors.lastname: Abdalla
authors.forename: J
authors.initials: J
authors.lastname: Gonzalez
authors.forename: C
authors.initials: C
authors.lastname: Garcia
authors.forename: J A
authors.initials: JA
authors.lastname: Bombi
authors.forename: M T
authors.initials: MT
authors.lastname: Jimenez de Anta
mesh_terms: D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D010088:Oxidoreductases / Q000032:analysis*; D014733:Vibrio / Q000201:enzymology / Q000302:isolation & purification* / Q000648:ultrastructure; D014734:Vibrio cholerae / Q000201:enzymology / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D003470:Culture Media; D010088:Oxidoreductases