title: Antigen receptor-mediated phosphoinositide hydrolysis in murine T cells is not initiated via G-protein activation.
abstract: Ligation of the antigen receptors on both T and B lymphocytes induces phosphoinositide (PI) hydrolysis, Ca(2+)-mobilization and protein kinase C activation. The activation of the phosphoinositide-specific phosphodiesterase (PPI-PDE) following crosslinking of surface Ig receptors on B cells is controlled by an uncharacterized guanine nucleotide-regulatory (G) protein. Here we have used permeabilized murine T cells (both resting T cells and a conalbumin-specific CD4-positive T cell clone) to investigate a role for G protein(s) in coupling the TCR to the PPI-PDE. We found that anti-TCR McAb (or processed antigen)-induced PI hydrolysis cannot be uncoupled by permeabilizing T cells, as occurs with classical G protein-linked receptors. Furthermore, the TCR-mediated release of inositol phosphates in permeabilized T cells was not enhanced by non-hydrolyzable analogs of GTP, nor inhibited by GDP analogs. These findings therefore argue strongly against the concept that TCR-mediated PI hydrolysis is G-protein controlled.
authors.affiliation: Division of Immunology, National Institute for Medical Research, Mill Hill, London, UK.
authors.forename: R J
authors.initials: RJ
authors.lastname: Phillips
authors.forename: M M
authors.initials: MM
authors.lastname: Harnett
authors.forename: G G
authors.initials: GG
authors.lastname: Klaus
mesh_terms: D000818:Animals; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D006868:Hydrolysis; D051379:Mice; D010716:Phosphatidylinositols / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D015398:Signal Transduction / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D010716:Phosphatidylinositols; D011948:Receptors, Antigen, T-Cell; D011505:Protein-Tyrosine Kinases; D019204:GTP-Binding Proteins