title: EPR characterization of the stereochemistry of the distal heme pocket of the engineered human myoglobin mutants.
abstract: Recombinant human myoglobin mutants with the distal histidine residue replaced by Leu, Val, or Gln residues have been prepared by site-directed mutagenesis and expression in Escherichia coli. The recombinant apomyoglobin proteins have been successfully reconstituted with cobaltous protoporphyrin IX to obtain cobalt myoglobin mutant proteins, and the role of the distal histidine residue on the interaction between the bound ligand and the myoglobin molecule has been studied by EPR spectroscopy. We found that the distal histidine residue is significant in the orientation of the bound oxygen molecule. Low temperature photolysis experiments on both oxy cobalt proteins and ferric nitric oxide complexes indicated that the nature of the photolyzed form depends on the steric crowding of the distal heme pocket. To our surprise, the distal Leu mutant has a less restricted, less sterically crowded distal heme pocket than that of the distal Val mutant myoglobin, despite the fact that Leu has a larger side chain volume than Val. Our results demonstrate that the distal heme pocket steric crowding is not necessarily related to the side chain volume of the E7 residue.
authors.affiliation: Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4970.
authors.forename: M
authors.initials: M
authors.lastname: Ikeda-Saito
authors.forename: R S
authors.initials: RS
authors.lastname: Lutz
authors.forename: D A
authors.initials: DA
authors.lastname: Shelley
authors.forename: E J
authors.initials: EJ
authors.lastname: McKelvey
authors.forename: R
authors.initials: R
authors.lastname: Mattera
authors.forename: H
authors.initials: H
authors.lastname: Hori
mesh_terms: D001665:Binding Sites; D003001:Cloning, Molecular; D003035:Cobalt; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D006418:Heme / Q000737:chemistry*; D006801:Humans; D016297:Mutagenesis, Site-Directed; D009211:Myoglobin / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D009211:Myoglobin; D011994:Recombinant Proteins; D003035:Cobalt; D006418:Heme