The presence of CO2/HCO3- is essential for hypoxic chemotransduction in the in vivo perfused carotid body.
Carotid chemoreceptor activity was increased by the perfusion of the carotid body in vivo with hypoxic HEPES-buffered solution (HBS) containing CO2/HCO3- (HBA+), but not with hypoxic HBS without CO2/HCO3- (HBS-). When the perfusate was switched to hypoxic HBS+ during hypoxic HBS-perfusions, chemoreceptor activity increased immediately. Thus, CO2/HCO3- played a critical role in the hypoxic chemotransduction of the in vivo perfused carotid body.
Shirahata
M
M
Department of Anesthesiology/Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, MD 21205.
Fitzgerald
R S
RS
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology*; D002245:Carbon Dioxide / Q000494:pharmacology*; D002344:Carotid Body / Q000502:physiology*; D002415:Cats; D002628:Chemoreceptor Cells / Q000187:drug effects / Q000502:physiology*; D000860:Hypoxia / Q000503:physiopathology; D007700:Kinetics; D010477:Perfusion; D015398:Signal Transduction; D013997:Time Factors
D001639:Bicarbonates; D002245:Carbon Dioxide