Protein kinases associated with proliferation and differentiation in murine erythroleukaemic cells.
1. The changes in electrophoretic distribution of cytosolic protein kinases have been studied in relation to proliferation, differentiation and transformation in murine erythroleukaemic cells, using a non-denaturing polyacrylamide gel electrophoresis system. 2. Native molecular masses of the major forms were determined by Ferguson plots. 3. A two dimensional electrophoresis method was developed for determination of the subunit molecular masses. 4. These studies suggest that the major bands of activity contain components which may correspond to cGMP dependent protein kinase, cAMP dependent protein kinase and protein kinase C. 5. On hexamethylene bisacetamide induced differentiation of the cells, changes in proportions of the different forms were observed.
Sprott
S C
SC
Department of Medical Biochemistry, University of the Witwatersrand Medical School, Parktown, Johannesburg, South Africa.
Hammond
K D
KD
Savage
N
N
D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000378:metabolism; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology / Q000473:pathology*; D051379:Mice; D008970:Molecular Weight; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*
D000081:Acetamides; D006657:Histones; D000242:Cyclic AMP; D011494:Protein Kinases; D011493:Protein Kinase C; D006152:Cyclic GMP; C014026:hexamethylene bisacetamide