F390 synthetase and F390 hydrolase from Methanobacterium thermoautotrophicum (strain delta H).
Factor F390 is the 8-OH adenylated form of the deazaflavin coenzyme F420, which is a central electron carrier in methanogenic bacteria. The enzymes catalysing the formation of F390 from ATP and F420 (F390 synthetase) and its hydrolysis into AMP and F420 (F390 hydrolase) were isolated and partially purified from Methanobacterium thermoautotrophicum. Both enzymes were oxygen-stable. The F390 synthetase tended to coelute with coenzyme F420 reducing hydrogenase during all purification steps. The 30-fold purified enzyme was still contaminated with the hydrogenase. The F390 hydrolase was purified 135-fold to a specific activity of 8.6 mumol/min/mg protein. The colourless enzyme consisted of one polypeptide of approximately 27,000 kd.
Kengen
S W
SW
Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
von den Hoff
H W
HW
Keltjens
J T
JT
van der Drift
C
C
Vogels
G D
GD
D004591:Electrophoresis, Polyacrylamide Gel; D019605:Euryarchaeota / Q000201:enzymology*; D008970:Molecular Weight; D009713:Nucleotidyltransferases / Q000737:chemistry / Q000302:isolation & purification*; D010727:Phosphoric Diester Hydrolases; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000302:isolation & purification*
C069619:F390 synthetase; D009713:Nucleotidyltransferases; D010744:Phosphoric Monoester Hydrolases; C069618:F390 hydrolase; D010727:Phosphoric Diester Hydrolases