ESR measurement of radical clearance in lung of whole mouse.
Clearance of the nitroxide radicals, hydroxy-TEMPO and carboxy-PROXYL, in whole-mouse lung was directly measured by in vivo ESR. After injecting a nitroxide radical, distribution of the nitroxide radical all over the lung was confirmed by ESR imaging. The ESR signal of hydroxy-TEMPO was reduced in the lung and the clearance obeyed first-order kinetics, whereas the signal of carboxy-PROXYL remained constant. Comparison of the clearance rates of live and dead mice indicated the presence of 2 different clearance systems in the lung: loss of its paramagnetism in the lung, and transfer from alveolar to the blood circulation system.
Takeshita
K
K
Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Utsumi
H
H
Hamada
A
A
D000818:Animals; D003497:Cyclic N-Oxides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005260:Female; D005609:Free Radicals; D008168:Lung / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D051379:Mice; D009132:Muscles / Q000378:metabolism; D009589:Nitrogen Oxides / Q000378:metabolism; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005609:Free Radicals; D009589:Nitrogen Oxides; D013113:Spin Labels; C032289:2,2,5,5-tetramethyl-1-pyrrolidinyloxy-3-carboxylic acid; C039900:nitroxyl; C001803:tempol