title: Coxsackie virus B4 infection of the mouse pancreas: I. Detection of virus-specific RNA in the pancreas by in situ hybridisation.
abstract: The pathology of Coxsackie virus B4 (CVB4) infection in a murine model was investigated by in situ hybridisation using a biotinylated cDNA probe derived from CVB4. During the acute phase of infection virus RNA sequences were detected in the exocrine pancreas of 60% of mice infected with a pancreotropic variant of CVB4. A positive hybridisation signal was observed in other organs in some animals including the heart and liver of 1 mouse 28 days after infection. The cellular distribution of virus RNA sequences corresponded well with the histological findings in most tissues. Possible causes for failure of hybridisation in some infected pancreases are discussed in conjunction with potential application of the technique in human pancreas biopsy samples.
authors.affiliation: Department of Immunology, Royal London Hospital Medical College, England.
authors.forename: C
authors.initials: C
authors.lastname: Vella
authors.forename: A J
authors.initials: AJ
authors.lastname: Easton
authors.forename: R P
authors.initials: RP
authors.lastname: Eglin
authors.forename: C L
authors.initials: CL
authors.lastname: Brown
authors.forename: L
authors.initials: L
authors.lastname: Perry
mesh_terms: D000818:Animals; D002460:Cell Line; D003384:Coxsackievirus Infections / Q000382:microbiology / Q000473:pathology*; D004195:Disease Models, Animal; D029822:Enterovirus B, Human / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009693:Nucleic Acid Hybridization; D010179:Pancreas / Q000382:microbiology / Q000473:pathology*; D010182:Pancreatic Diseases / Q000382:microbiology / Q000473:pathology*; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D012367:RNA, Viral