A monoclonal antibody blocking ELISA detects antibodies specific for epizootic haemorrhagic disease virus.
The isolation of a monoclonal antibody (1G9/C9) with specificity for the epizootic haemorrhagic disease (EHD) serogroup has enabled the development of a highly sensitive and specific blocking ELISA (B-ELISA) for the detection of serum antibodies to EHD viruses. The assay was sensitive to blocking antibodies present in hyperimmune reference antisera to all six EHD serotypes tested but was unaffected by reference antisera to 19 South African and eight Australian serotypes of the related orbivirus bluetongue virus (BTV). The sensitivity of the EHD B-ELISA exceeded that of an indirect ELISA (I-ELISA) for EHD-specific antibody detection. Serum antibody titres to BTV and EHD in experimental and field sera, including a sentinel herd from which virus isolations were made, were examined in both the BTV and EHD B-ELISA tests. These results showed the B-ELISA was only sensitive to antibodies specific for the homologous serogroup in each case, even where sequential and mixed infections with each virus type occurred.
White
J R
JR
CSIRO, Australian Animal Health Laboratory, Geelong, Vic. Australia.
Blacksell
S D
SD
Lunt
R A
RA
Gard
G P
GP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D001667:Binding, Competitive; D015153:Blotting, Western; D001820:Bluetongue virus / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D007106:Immune Sera / Q000276:immunology; D008297:Male; D011237:Predictive Value of Tests; D015531:Radioimmunoprecipitation Assay; D012087:Reoviridae / Q000276:immunology*; D012088:Reoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D012756:Sheep
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007106:Immune Sera