[Interconnection of physico-chemical characteristics of organic solvents and their denaturing ability in relation to proteins].
Reversible denaturation of several proteins (alpha-chymotrypsin, trypsin, laccase, chymotrypsinogen, cytochrome c and myoglobin) by a broad series of organic solvents of different nature was studied. The regularities of this process were analyzed, employing both experimental and literary data based on the results of kinetic and spectroscopic measurements. In all the systems under study denaturation proceeded in a threshold manner, i. e., an abrupt change in the catalytic and/or spectroscopic properties of the dissolved proteins was observed after a certain threshold concentration of the organic solvent had been reached. To account for the observed features of the denaturation process, a thermodynamic model of reversible protein denaturation by organic solvents was proposed. This model is based on the widely accepted viewpoint that the undisturbed water shell around the protein globule is necessary for maintaining the dissolved protein in the native state. Quantitative analysis of the model led to an equation establishing a relationship between the threshold concentration of an organic solvent and its physico-chemical characteristics, such as hydrophobicity, solvating ability and molecular geometry. This equation fits well in the experimental data for all the proteins tested. Based on the above thermodynamic model of protein denaturation, a novel quantitative parameter characterizing the denaturing strength of organic solvents (termed as the denaturation capacity or DC) was proposed. Different organic solvents arranged according to their DC values form the DC scale of organic solvents which permits to predict theoretically the threshold concentration of any organic solvent for a given protein.(ABSTRACT TRUNCATED AT 250 WORDS)
Belova
A B
AB
Mozhaev
V V
VV
Levashov
A V
AV
Sergeeva
M V
MV
Martinek
K
K
Khmel'nitskiĭ
Iu L
IuL
D000818:Animals; D002384:Catalysis; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D042845:Laccase; D009211:Myoglobin / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D011489:Protein Denaturation; D012997:Solvents; D013816:Thermodynamics; D014357:Trypsin / Q000378:metabolism
D003574:Cytochrome c Group; D009211:Myoglobin; D012997:Solvents; D010088:Oxidoreductases; D042845:Laccase; D002918:Chymotrypsin; D014357:Trypsin
Vzaimosviaz' fiziko-khimicheskikh kharakteristik organicheskikh rastvoriteleĭ s ikh denaturiruiushcheĭ sposobnost'iu po otnosheniiu k belkam.