Antiidiotypic responses to immunization with anti-Leu 3a in human immunodeficiency virus-seropositive individuals.
Anti-idiotypic antibodies to anti-CD4 monoclonal antibodies (MAbs) have been reported to bind to the human immunodeficiency virus (HIV) envelope glycoprotein gp120. To establish whether HIV-infected patients can mount an anti-idiotypic response to murine MAb, four individuals with symptoms of AIDS-related complex (ARC) were immunized over 10 weeks with six intramuscular injections of anti-Leu 3a (1 mg). Despite their immunocompromised state, all patients made anti-constant region and anti-idiotypic antibodies, although the amplitude of the responses varied between individuals. No significant toxic or allergic reactions were seen, and there were no changes in clinical status during the 6 months after immunization. No increase in serum HIV-neutralization titers was seen, and purified anti-idiotypic antibodies did not bind gp120.
Wilks
D
D
Division of Immunology, Clinical Research Centre, Harrow, United Kingdom.
Walker
L C
LC
Habeshaw
J A
JA
Webster
A D
AD
Dalgleish
A G
AG
D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000009:adverse effects / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D006679:HIV Seropositivity / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D015699:HIV Envelope Protein gp120; D007074:Immunoglobulin G; D007075:Immunoglobulin M