Detection of human herpesvirus 6 DNA in T and B cell lymphomas.
DNA extracted from tissues of 109 patients with lymphoproliferative disorders, including 40 T-cell lymphomas, 40 B-cell lymphomas, 8 Hodgkin's disease, 13 hyperplasia, 5 infectious mononucleosis, 2 histiocytic medullary reticulosis and 1 Kikuchi's disease, and peripheral blood lymphocytes (PBL) from 17 healthy donors were investigated for the presence of human herpesvirus 6 (HHV-6) DNA sequence by polymerase chain reaction (PCR) and Southern hybridization. The procedures for DNA amplification were composed of the first round of PCR using a pair of primers LL and LR, which were contained in the HHV-6 major capsid gene, and the second round of nested PCR using an internal pair of primers LLN and LRN. Results showed that the prevalence of HHV-6 DNA sequence in human tissues was similar in both B and T cell lymphoma patients and normal adults, and viral heterogeneity was detected in the amplified DNA sequence from local strain of Taiwan as compared to that of U1102 strain from Uganda.
Chen
J Y
JY
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Cheng
C Y
CY
Hsu
T Y
TY
Su
I J
IJ
Pao
C C
CC
Liu
M Y
MY
Yang
C S
CS
D004279:DNA, Viral / Q000032:analysis*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000382:microbiology*; D016399:Lymphoma, T-Cell / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012016:Reference Values; D012680:Sensitivity and Specificity
D004279:DNA, Viral