Semiquantitative PCR in the diagnosis of HIV infection: correlation to clinical stage and serological status.
Authors utilized the polymerase chain reaction (PCR) technique to detect the presence of HIV DNA in 31 subjects (12 seropositive patients, 4 seronegative, at risk persons and 15 seronegative, not at risk controls). PCR was highly sensitive (enabling the detection of as few as 10 proviral genomes) and specific. By comparison to known amounts of HIV DNA, it was possible to obtain semiquantitative evaluation. No correlation was found between the proviral amount and the clinical stage of the disease or the p24 antigenemia.
De Socio
G V
GV
II Institute of Obstetrics and Gynecology, University La Sapienza, Rome, Italy.
Falcinelli
C
C
Francisci
D
D
Pauluzzi
S
S
Cosmi
E V
EV
Anceschi
M M
MM
D000328:Adult; D002648:Child; D004279:DNA, Viral / Q000097:blood*; D016679:Genome, Viral; D006678:HIV / Q000235:genetics / Q000302:isolation & purification*; D015483:HIV Antibodies / Q000097:blood; D016655:HIV Core Protein p24 / Q000097:blood; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy; D006801:Humans; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*; D012307:Risk Factors; D012680:Sensitivity and Specificity; D015819:Substance Abuse, Intravenous / Q000097:blood; D015215:Zidovudine / Q000627:therapeutic use
D004279:DNA, Viral; D015483:HIV Antibodies; D016655:HIV Core Protein p24; D015215:Zidovudine