Comparison of human and porcine group C rotaviruses by northern blot hybridization analysis.
The genetic relationship between human and porcine Gp C rotaviruses and between Gp C and Gp A or B rotaviruses was examined by Northern blot hybridization. Cross-hybridization studies using radiolabeled ssRNA transcript probes demonstrated that the human and porcine Gp C rotaviruses shared a high degree of nucleotide sequence homology in most of the eleven gene segments; the greatest sequence divergence was observed in gene 7. Neither the human nor the porcine Gp C probe hybridized strongly with gene segments from Gp A reference strains or a Gp B bovine rotavirus. These data indicate that genetically, porcine and human Gp C rotaviruses are closely related, whereas they are quite distinct from Gp A or B suggesting that porcine and human Gp C rotaviruses may have evolved from a common ancestral source.
Qian
Y
Y
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Saif
L J
LJ
Kapikian
A Z
AZ
Kang
S Y
SY
Jiang
B
B
Ishimaru
Y
Y
Yamashita
Y
Y
Oseto
M
M
Green
K Y
KY
D000818:Animals; D015152:Blotting, Northern; D005814:Genes, Viral; D006801:Humans; D015347:RNA Probes; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000382:microbiology
D015347:RNA Probes; D012367:RNA, Viral