Macrophage containing factor XIII subunit a in salivary glands of patients with Sjögren's syndrome.
Minor labial salivary glands obtained at biopsy from patients with Sjögren's syndrome were investigated by immunomorphological methods for the presence of monocyte-derived macrophages. According to our observations published earlier the immunomorphological detection of factor XIII subunit a is a useful marker for recognizing cells of monocyte/macrophage lineage. Factor XIII subunit a was detected by a highly sensitive immunoperoxidase staining, and cells containing this coagulation enzyme were characterized by double immunofluorescence stainings. Factor XIII subunit a+ cells were found to be highly accumulated at the interface of normal tissue and peritubular infiltrate. In double immunofluorescence labelling systems factor XIII subunit a+ cells were simultaneously labelled by RFD7 and Dako-antimacrophage monoclonal antibodies. They also expressed HLA-DR antigen as revealed by a reaction with RFDR2 monoclonal antibody. The results suggest that monocyte-derived tissue macrophages are present in salivary glands of patients with Sjögren's syndrome and have a characteristic distribution. It can be assumed that they have a role in the demarcation of peritubular inflammation and thus have an effect on the progression of the disease.
Zeher
M
M
3rd Department of Medicine, University Medical School, Debrecen, Hungary.
Adány
R
R
Nagy
G
G
Gómez
R
R
Szegedi
G
G
D000328:Adult; D000368:Aged; D005176:Factor XIII / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000276:immunology* / Q000473:pathology; D008875:Middle Aged; D012469:Salivary Glands / Q000276:immunology* / Q000473:pathology; D012859:Sjogren's Syndrome / Q000276:immunology* / Q000473:pathology
D006684:HLA-DR Antigens; C064015:factor XIII subunit A; D005176:Factor XIII