[Agglutinating and immobilizing activity of an ethanol extract of Bursera fagaroides on human and other mammalian spermatozoa].
Human spermatozoa and those obtained from mouse epididymis incubated in ethanolic from mouse epididymis incubated in ethanolic extracts of the cortex of Bursera fagaroides became agglutinated and immobilized in 100% with 30% of viability decrease. This double effect occurred in 50% of pig'ssperm and 50% of rabbit sperm when the concentration was increased tenfold. The pattern of progressive motility was affected 80% of rat's spermatocytes without agglutination. The values remained the same when the extracts were partially purified with salts of Zn++ and Ba++ or by filtration in a sephadex g-10 column. Analysis with thin layer chromatography of both purified extracts produced 3 compounds in comparison with the ethanolic unpurified extract. The extract of the plant Bursera fagaroides contains 3 compounds, which apparently are glycosides with a potent activity upon agglutination -immobilization and a low effect upon spermatocytes viability, which might be used as contraceptives .
Huacuja
R L
RL
Unidad de Inv. Biomédica de Occidente, IMSS, Guadalajara, Jal., México.
Delgado
N M
NM
Carranco
L A
LA
Reyes
L R
LR
Rosado
G A
GA
D000818:Animals; D003272:Contraceptive Agents, Male; D000431:Ethanol; D006801:Humans; D008297:Male; D008800:Mexico; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013073:Sperm Agglutination / Q000187:drug effects*; D013081:Sperm Motility / Q000187:drug effects*; D013552:Swine
D003272:Contraceptive Agents, Male; D010936:Plant Extracts; D000431:Ethanol
Actividad aglutinante e inmovilizante del extracto etanólico de Bursera fagaroides sobre espermatozoides de humano y de otros mamíferos.