Sodium nitroprusside degenerates cultured rat striatal neurons.
Incubation of a primary culture of rat striatal neurons with sodium nitroprusside (SNP), a known source of nitric oxide (NO), showed a concentration-dependent increase in cyclic GMP levels with an EC50 value of 13.7 microM. Twenty-four hours following incubation with 10 microM SNP, striatal neurons underwent degeneration as assessed immunohistochemically and biochemically. In contrast, potassium ferricyanate at concentrations up to 1 mM had no effect on striatal neuron viability. These results indicate that SNP has neurotoxic actions in-vitro, an effect that may involve NO as a second messenger.
Chen
J
J
Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, Illinois 60064-3500.
Chang
B
B
Williams
M
M
Murad
F
F
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects*; D003470:Culture Media; D006152:Cyclic GMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009410:Nerve Degeneration; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology*; D009994:Osmolar Concentration; D051381:Rats
D003470:Culture Media; D009599:Nitroprusside; D007770:L-Lactate Dehydrogenase; D006152:Cyclic GMP