Immunolesioning: selective destruction of neurons using immunotoxin to rat NGF receptor.
192 IgG, a monoclonal antibody to the rat nerve growth factor (NGF) receptor, was disulfide-coupled to saporin, a ribosome-inactivating protein. Systemic injection of 192 IgG-saporin destroyed sympathetic postganglionic neurons and some sensory neurons. Injection of 192 IgG-saporin into the lateral ventricle destroyed cholinergic neurons of the basal forebrain. These results show that antineuronal immunotoxins are a powerful approach that may prove useful in a variety of neurobiological applications.
Wiley
R G
RG
Neurology Service, DVAMC, Nashville, TN 37212-2637.
Oeltmann
T N
TN
Lappi
D A
DA
D000818:Animals; D000911:Antibodies, Monoclonal; D002470:Cell Survival / Q000187:drug effects; D007839:Functional Laterality; D005728:Ganglia, Sympathetic / Q000187:drug effects / Q000473:pathology*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D000922:Immunotoxins / Q000032:analysis / Q000633:toxicity*; D009699:N-Glycosyl Hydrolases; D009414:Nerve Growth Factors / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D009475:Neurons, Afferent / Q000187:drug effects / Q000473:pathology; D010940:Plant Proteins / Q000633:toxicity*; D051381:Rats; D011956:Receptors, Cell Surface / Q000276:immunology*; D017475:Receptors, Nerve Growth Factor; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D000922:Immunotoxins; D009414:Nerve Growth Factors; D010940:Plant Proteins; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins