The role of cyclins in the maturation of Patella vulgata oocytes.
We have cloned and sequenced the cDNAs encoding Patella vulgata cyclins A and B. The cDNA clones contain an open reading frame of 426 and 408 amino acids respectively, which present similarity with cyclins from other species. Cyclin A and B RNAs are present as polyadenylated and non-polyadenylated RNA in prophase oocytes and are completely polyadenylated in metaphase I. During the first cleavages after fertilization the level of cyclin A and B mRNAs is high and drops when the free swimming stage is reached. Using p13suc1-Sepharose bead precipitation we demonstrate that cyclin synthesis is triggered during maturation and that inhibition of protein synthesis makes the cyclins disappear rapidly from the metaphase I oocytes, which shift to interphase condition. By microinjecting antisense oligonucleotides into metaphase I oocytes, we demonstrate that in vivo ablation of cyclin A and B messengers together gives the same result, whereas microinjection of only one oligonucleotide does not show any effect.
van Loon
A E
AE
Ecole Normale Superieure, Laboratoire de Biologie Cellulaire et Moleculaire, Lyon, France.
Colas
P
P
Goedemans
H J
HJ
Néant
I
I
Dalbon
P
P
Guerrier
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D016213:Cyclins / Q000502:physiology*; D004247:DNA / Q000235:genetics; D006801:Humans; D016200:Maturation-Promoting Factor / Q000378:metabolism; D008677:Metaphase; D008845:Microinjections; D008969:Molecular Sequence Data; D008974:Mollusca / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D010063:Ovum / Q000254:growth & development*; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000378:metabolism; D016914:Ribonuclease H; D013045:Species Specificity
D016213:Cyclins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D004247:DNA; D016200:Maturation-Promoting Factor; D016914:Ribonuclease H