Detection of mouse hepatitis virus antibody by protein A-ELISA in 6 prevalent inbred strains or outbred stocks of mice.
Protein A was applied as a reagent for the secondary reaction in ELISA (protein A-ELISA). Mouse hepatitis virus antibody in 6 prevalent mouse strains or stocks reared in a MHV-contaminated room was effectively detected by protein A-ELISA, whereas significant strain differences in the antibody detection rate were demonstrated using the complement fixation test. C57BL/6 mice were particularly reactive in the protein A-ELISA test.
Kagiyama
N
N
Department of Laboratory Animal Medicine, Central Institute for Experimental Animals, Kawasaki, Japan.
Takakura
A
A
Koyama
K
K
Terada
E
E
Sakurai
Y
Y
D000822:Animal Husbandry; D000818:Animals; D000830:Animals, Laboratory; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006524:Hepatitis, Viral, Animal / Q000175:diagnosis* / Q000276:immunology; D051379:Mice / Q000382:microbiology*; D008815:Mice, Inbred Strains / Q000382:microbiology*; D006517:Murine hepatitis virus / Q000276:immunology* / Q000302:isolation & purification; D013045:Species Specificity; D013205:Staphylococcal Protein A
D000914:Antibodies, Viral; D013205:Staphylococcal Protein A