Induction of neurite outgrowth of PC12 cells by an inhibitor of vacuolar H(+)-ATPase, bafilomycin A1.
Bafilomycin A1, a selective inhibitor of vacuolar H(+)-ATPase, induced neurite outgrowth of PC12 cells dose- and time-dependently: more than 50% of the cells extended neurite-like spikes after 24 h treatment with 100 nM bafilomycin A1. Its dose-response ran roughly parallel to that of a bafilomycin A1-induced lysosomal pH increase. It was inhibited by LiCl, an inhibitor of the phosphorylation of microtubule-associated proteins and, like nerve growth factor (NGF)-induced neurite outgrowth, it was also inhibited by cycloheximide and actinomycin D. But, unlike the NGF-effect, it was not associated with rapid induction of c-fos.
Tamura
H
H
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Ohkuma
S
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D016762:Genes, fos; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D018942:Macrolides; D009414:Nerve Growth Factors / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D016716:PC12 Cells; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D014617:Vacuoles / Q000201:enzymology*
D000900:Anti-Bacterial Agents; D002712:Chlorides; D018942:Macrolides; D009414:Nerve Growth Factors; D003609:Dactinomycin; D003994:Bucladesine; C040929:bafilomycin A1; D003513:Cycloheximide; D008094:Lithium; D006180:Proton-Translocating ATPases; D018021:Lithium Chloride