Molecular cloning of provirus DNA from bovine leukaemia lymphocytes and its application as a probe for diagnostic purpose.
The provirus DNA isolated from lymphocytes of a cow infected with the bovine leukaemia virus (BLV; positive immunodiffusion test), was subjected to molecular cloning and identified by comparing with the 32P-labelled provirus cDNA isolated in Belgium. Hybridization revealed a clone containing 8.5 kb DNA fragment of the BLV provirus. The probe based on the "Polish fragment" of leukaemia virus was tested on 10 cows with a positive serological response. The presence of provirus DNA in the cellular genome of lymphocytes was confirmed.
Reichert
M
M
Department of Biochemistry, Institute of Veterinary Science Puławy, Poland.
Grundboeck-Juśko
J
J
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D007938:Leukemia / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D008214:Lymphocytes / Q000737:chemistry*; D011533:Proviruses / Q000235:genetics*
D015342:DNA Probes; D004279:DNA, Viral