title: Ki-ras activation in pancreatic carcinomas of Syrian hamsters induced by N-nitrosobis(2-hydroxypropyl)amine.
abstract: Five pancreatic carcinomas induced by N-nitrosobis(2-hydroxypropyl)amine in Syrian golden hamsters were analyzed for activation of Ki-ras at codons 12 and 13, using the polymerase chain reaction and direct sequencing. The Ki-ras gene was shown to be activated in four of the five carcinomas, and the results were further confirmed by subcloning and sequencing. All the mutations involved a G-to-A transition at the second position of codon 12, which resulted in a change at the amino acid level from glycine to aspartic acid. This mutation is identical with that reported for pancreatic tumors of Syrian hamsters induced by N-nitrosobis(2-oxopropyl)amine.
authors.affiliation: Carcinogenesis Division, National Cancer Center Research Institute, Tokyo.
authors.forename: T
authors.initials: T
authors.lastname: Ushijima
authors.forename: M
authors.initials: M
authors.lastname: Tsutsumi
authors.forename: R
authors.initials: R
authors.lastname: Sakai
authors.forename: Y
authors.initials: Y
authors.lastname: Ishizaka
authors.forename: F
authors.initials: F
authors.lastname: Takaku
authors.forename: Y
authors.initials: Y
authors.lastname: Konishi
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Sugimura
authors.forename: M
authors.initials: M
authors.lastname: Nagao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002273:Carcinogens; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000139:chemically induced / Q000235:genetics*; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D011905:Genes, ras / Q000187:drug effects*; D008647:Mesocricetus; D008969:Molecular Sequence Data; D009602:Nitrosamines; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000235:genetics*
chemical_list: D002273:Carcinogens; D004273:DNA, Neoplasm; D009602:Nitrosamines; C012457:diisopropanolnitrosamine