title: [A sensitive enzyme-linked immunosorbent assay (ELISA) for the serological detection of antibodies against the European hog cholera virus].
abstract: An ELISA for the detection of antibodies against hog cholera virus (HCV) was developed. The HCV-specific glycoprotein gp53 served as diagnostic antigen after immobilization using a monoclonal capture antibody. Due to the higher affinity of HCV-specific antibodies to the viral gp53, sera cross reacting with bovine viral diarrhea (BVD) virus were discriminated by the slope of the titration curves.
authors.affiliation: Institut für Virologie, Tierärztlichen Hochschule, Hannover.
authors.forename: G
authors.initials: G
authors.lastname: Schagemann
authors.forename: I
authors.initials: I
authors.lastname: Greiser-Wilke
authors.forename: B
authors.initials: B
authors.lastname: Liess
authors.forename: V
authors.initials: V
authors.lastname: Moennig
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D003429:Cross Reactions; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral
vernacular_title: Ein empfindlicher Enzyme-linked Immunosorbent Assay (ELISA) zum serologischen Nachweis von Antikörpern gegen das Virus der Europäischen Schweinepest (ESPV).