title: Calyculin A increases voltage-dependent inward current in smooth muscle cells isolated from guinea pig taenia coli.
abstract: The effects of a potent phosphatase inhibitor, calyculin A (CL-A), on inward currents in guinea pig taenia coli smooth muscle cells were examined. CL-A increased the inward current, and this effect of CL-A was inhibited by a protein kinase C inhibitor, H-7, and by nifedipine. Phorbol 12,13-dibutyrate, an activator of protein kinase C, also increased the inward current and this effect was antagonized by H-7. These results suggest that in guinea pig taenia coli smooth muscle cells CL-A may facilitate the opening of the L-type Ca2+ channels through the protein kinase C-dependent phosphorylation system.
authors.affiliation: Department of Physiology, Sapporo Medical College, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Usuki
authors.forename: K
authors.initials: K
authors.lastname: Obara
authors.forename: T
authors.initials: T
authors.lastname: Someya
authors.forename: H
authors.initials: H
authors.lastname: Ozaki
authors.forename: H
authors.initials: H
authors.lastname: Karaki
authors.forename: N
authors.initials: N
authors.lastname: Fusetani
authors.forename: H
authors.initials: H
authors.lastname: Yabu
mesh_terms: D000818:Animals; D001464:Barium / Q000502:physiology; D015220:Calcium Channels / Q000187:drug effects* / Q000502:physiology; D002469:Cell Separation; D003106:Colon; D006168:Guinea Pigs; D008387:Marine Toxins; D008564:Membrane Potentials / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D010080:Oxazoles / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors
chemical_list: D015220:Calcium Channels; D008387:Marine Toxins; D010080:Oxazoles; D001464:Barium; C059041:calyculin A; D011493:Protein Kinase C