A reverse-phase HPLC method for cAMP phosphodiesterase activity.
A simple and fast method based on reverse-phase HPLC has been developed for measuring the activity of cAMP phosphodiesterase. It allows quantitation of product and substrate in less than 10 min. The sensitivity (1*10(-11) mol AMP), the accurate evaluation of nucleotides, the unequivocal analysis of product, and the reproducibility of the system, make this method suitable for the evaluation of cAMP phosphodiesterase in biological material, at different levels of purification, and also in kinetic studies.
Spoto
G
G
Institute of Biochemical Sciences, University of Chieti G. D'Annunzio, Italy.
Whitehead
E
E
Ferraro
A
A
Di Terlizzi
P M
PM
Turano
C
C
Riva
F
F
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007700:Kinetics; D008832:Microchemistry / Q000379:methods; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results
D015105:3',5'-Cyclic-AMP Phosphodiesterases