Sequence of the VP7 gene of chicken rotavirus Ch2 strain of serotype 7 rotavirus.
The gene that encodes the VP7 protein of the Ch2 strain of group A chicken rotavirus (serotype G7) was sequenced, and its deduced amino acid sequence analyzed. The gene is 1067 bp in length and contains a single long open reading frame of 987 nt, capable of encoding a protein of 329 amino acids. Amino acid homology of the chicken rotavirus VP7 to the corresponding protein of rotaviruses representing each of the other twelve G serotypes ranged from 58 to 63%. In addition, the VP7 protein of the Ch2 strain contains a unique amino acid sequence in variable regions VR5 (A), VR7 (B), or VR8 (C), which is consistent with previous studies indicating that these three regions are involved in G serotype specificity. The VP7 protein had amino acid homology of 19 or 33% with that of groups B (ADRV) or C (Cowden) rotavirus strains, respectively. Attempts to produce reassortants in vivo or in vitro between the Ch2 strain and strains belonging to group A or C rotavirus were unsuccessful. The low level of nucleotide identity in the 5' end noncoding region of the VP7 gene between the Ch2 and the group A or C rotaviruses may account for this failure.
Nishikawa
K
K
Epidemiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Hoshino
Y
Y
Gorziglia
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002645:Chickens; D003001:Cloning, Molecular; D005814:Genes, Viral / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D012401:Rotavirus / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping
D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus