title: Estimation of avidin activity by two methods.
abstract: The biological activity of avidin was estimated by two different methods. The spectrophotometric method used the avidin titration with biotin in the presence of 4 hydroxiazobenzen-2'carboxilic acid as indicator. In the radioisotopic determination the titration with tritiated biotin was accomplished. Both methods led to the same results, but the spectrophotometric one is less avidin expensive and more rapid, being more convenient.
authors.affiliation: Faculty of Chemistry, University of Bucharest, Romania.
authors.forename: B
authors.initials: B
authors.lastname: Borza
authors.forename: F
authors.initials: F
authors.lastname: Marcheş
authors.forename: R
authors.initials: R
authors.lastname: Repanovici
authors.forename: O
authors.initials: O
authors.lastname: Burducea
authors.forename: L M
authors.initials: LM
authors.lastname: Popa
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001360:Avidin / Q000032:analysis* / Q000302:isolation & purification; D001391:Azo Compounds / Q000138:chemical synthesis; D001710:Biotin; D005069:Evaluation Studies as Topic; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D007202:Indicators and Reagents; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000914:Antibodies, Viral; D001391:Azo Compounds; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D001360:Avidin; C001413:HABA; D001710:Biotin