title: Decreased binding capacity for alpha-human atrial natriuretic peptide in aldosterone-producing adrenocortical adenoma.
abstract: The previous study demonstrated that the aldosterone secretion of aldosterone-producing adrenocortical adenoma failed to be suppressed by human atrial natriuretic peptide, based on the data in synthetic alpha-human atrial natriuretic peptide infusion in vivo and the effect on in vitro secretion from cultured adenoma cells. Using the membrane fractions prepared from human adrenal tissues and an aldosterone-producing adenoma tissues, we characterized the binding sites for [125I] alpha-human atrial natriuretic peptide by the binding study and affinity-labeling of [125I] alpha-human atrial natriuretic peptide. Both normal adrenal and adenoma tissue membrane fractions possessed specific binding sites, however the relative binding capacity for [125I] alpha-human atrial natriuretic peptide in the adenoma preparations was markedly lower than that in the normal adrenal tissue preparation. The specific binding sites for [125I] alpha-human atrial natriuretic peptide were detected at the region of 140 KD and 67-70 KD only in the normal adrenal membrane fractions. Our data suggest that the refractoriness to the effect of atrial peptide may be due to the reduced receptor sites in aldosterone-producing adenoma cells.
authors.affiliation: Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka.
authors.forename: M
authors.initials: M
authors.lastname: Ohashi
authors.forename: K
authors.initials: K
authors.lastname: Higuchi
authors.forename: T
authors.initials: T
authors.lastname: Hashiguchi
authors.forename: R
authors.initials: R
authors.lastname: Takayanagi
authors.forename: H
authors.initials: H
authors.lastname: Nawata
mesh_terms: D000236:Adenoma / Q000378:metabolism*; D000306:Adrenal Cortex Neoplasms / Q000378:metabolism*; D000450:Aldosterone / Q000096:biosynthesis; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001665:Binding Sites; D006801:Humans; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D011956:Receptors, Cell Surface; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor