A comparison of rodent caging systems based on microenvironmental parameters.
Four different mouse caging systems were evaluated for microenvironmental temperature, carbon dioxide, relative humidity (RH) and ammonia levels during a 7-day testing period. All caging systems evaluated had polycarbonate bases and consisted of either a molded polyester (MP) filter lid, one of two different polycarbonate filter lids, or no filter lid which served as a control. At 50% macroenvironmental RH (study I), all systems maintained an intracage temperature of 75.5 degrees F +/- 0.5 degrees. Both polycarbonate systems averaged greater than 2200 ppm of carbon dioxide more than the MP system and the controls. When compared with RH in the control cages, RH levels averaged over 20% and 5 to 8% RH greater in the polycarbonate filter lid systems and the MP system, respectively. There were no appreciable ammonia levels in either the MP or control systems. In the polycarbonate filter lid systems, ammonia levels were detectable on day 4 and were greater than 200 ppm by day 6. At 20% macroenvironmental RH (study II), there was a proportional 15 to 30% RH decrease from study I levels. Ammonia levels were undetectable in any system until day 7 and averaged only 17 ppm in one of the polycarbonate systems. Minimal differences were observed in studies III, IV and V when pine shavings were used instead of hardwood chips, a CD-1 stock instead of a DBA/2J strain, and different grades of filter inserts in the polycarbonate systems, respectively.
Corning
B F
BF
Division of Comparative Medicine, Massachusetts Institute of Technology Cambridge 02139.
Lipman
N S
NS
D000393:Air Pollutants / Q000032:analysis; D000641:Ammonia / Q000032:analysis; D000822:Animal Husbandry / Q000295:instrumentation*; D000818:Animals; D002245:Carbon Dioxide / Q000032:analysis; D004780:Environment, Controlled; D005069:Evaluation Studies as Topic; D005260:Female; D006813:Humidity; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR
D000393:Air Pollutants; D002245:Carbon Dioxide; D000641:Ammonia