Production of a transforming growth factor-beta-like growth factor by RSV-transformed rat cerebral microvascular endothelial cells.
This study describes the preliminary characterization of a Rous sarcoma virus (RSV)-transformed rat cerebral microvascular endothelial cell (RCE-T1)-derived growth factor with biological properties similar to transforming growth factor-beta (TGF-beta). A significant portion of this RCE-T1-derived TGF-beta-like growth factor was secreted in an active form and its secretion was elevated 2- to 3-fold in late passage cultures comprised of rapidly growing, less differentiated cells exhibiting reduced sensitivity to the growth-inhibitory effects of exogenous TGF-beta 1. These data suggest that the RCE-T1 cell line may be a useful system in which to study the role of autocrine TGF-beta production in normal and aberrant endothelial cell growth.
Mooradian
D L
DL
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis.
Diglio
C A
CA
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000502:physiology*; D001921:Brain / Q000098:blood supply*; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000502:physiology; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D005347:Fibroblasts / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D008833:Microcirculation; D051381:Rats; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000096:biosynthesis*
D003470:Culture Media; D016212:Transforming Growth Factor beta; D004247:DNA