[Obtaining transgenic Solanum tuberosum potato plants with active bacterial genes xyl and T-cyt effecting the phytohormone balance].
The genetic constructions based on integrative vector pGV3850 were used to introduce bacterial genes xyl and T-cyt into potato cells. The transformation was carried out using the leaf-disc method with modifications. A special system for obtaining regenerants from explants of potato in vitro plants or calli has been designed that permitted the selection of transgenic shoots. The presence of the genes in potato genome has been proved by testing the NPTII and glucoisomerase activities. The transgenic plants expressing T-cyt gene differed from the wild type in sharp decrease of the apical dominance.
Krasheninnikova
L V
LV
Rassadina
G V
GV
Kirsanova
S N
SN
Khromova
L M
LM
Iusibov
V M
VM
Pak Chun
I r
Ir
Andrianov
V M
VM
Piruzian
E S
ES
D016960:Agrobacterium tumefaciens / Q000235:genetics; D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D019868:Kanamycin Kinase; D010770:Phosphotransferases / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism
D010937:Plant Growth Regulators; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase
Poluchenie transgennykh rasteniĭ kartofelia Solanum tuberosum, nesushchikh aktivnye bakterial'nye geny xyl i T-cyt, vliiaiushchie na balans fitogormonov.