Calibration of [123I]iodine-labeled tissue standards for autoradiographic studies.
Autoradiographic images can be analyzed with computer-assisted microdensitometry relative to radioactive reference standards to provide quantitative measurements of regional radioactivity concentrations. [123I]Iodine containing sections of brain paste have been calibrated relative to plastic-embedded tritium(3H) and 125Iodine standards. For exposure times of 8, 12 and 24 h, plastic-embedded standards covered a range concentrations of [123I]iodine from 1000 to 100,000 dpm/mg wet weight. Iodine-123 radioactivity was linear with section thickness from 5 to 30 microns. These studies confirmed the feasibility of using commercially available longer-lived reference standards to provide [123I]iodine equivalent values in absolute units of dpm (or microCi) per mg tissue.
Johnson
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EW
Department of Psychiatry, Yale University School of Medicine, West Haven, CT 06510.
Sybirska
E
E
al-Tikriti
M
M
Innis
R B
RB
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001923:Brain Chemistry; D002138:Calibration; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D014316:Tritium
D007457:Iodine Radioisotopes; D014316:Tritium