title: The induction of corticosteroid actions on membrane properties of hippocampal CA1 neurons requires protein synthesis.
abstract: Corticosterone can affect electrical properties of CA1 pyramidal neurons via binding to two corticoid receptor types, the mineralocorticoid (MR) and glucocorticoid receptor (GR). Previously we have shown that MR-activation leads to attenuation of serotonin (5-HT)-induced membrane hyperpolarization, while GR-activation induces an increase in the amplitude of the afterhyperpolarization (AHP) following a short current pulse. In this study we show that the MR- and GR-mediated changes of the membrane properties are prevented in the presence of the protein synthesis inhibitor cycloheximide, thus suggesting a genomic action of the steroids.
authors.affiliation: Institute of Molecular Biology, University of Utrecht, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Karst
authors.forename: M
authors.initials: M
authors.lastname: Joëls
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D003345:Corticosterone / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D015735:Mifepristone / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011712:Pyramidal Tracts / Q000166:cytology / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid / Q000187:drug effects / Q000378:metabolism; D012701:Serotonin / Q000494:pharmacology
chemical_list: D009419:Nerve Tissue Proteins; D011965:Receptors, Glucocorticoid; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid; D015735:Mifepristone; D012701:Serotonin; D003513:Cycloheximide; D003345:Corticosterone