VIP stimulation of polarized macromolecule secretion in cultured chick embryonic retinal pigment epithelium.
Vasoactive intestinal peptide (VIP) stimulated macromolecule secretion at the apical membranes of the chick embryonic retinal pigment epithelium cultured on permeable supports in a time- and concentration-dependent manner. VIP stimulated secretion of molecules with MW of 80, 74, 70, 60, 42, 35, 24, 20, and 14 kDa. A 1.9- to 2.6-fold stimulation in secretion of molecules with MW greater than 10 kDa precipitable by 10% trichloroacetic acid was observed after treatment with 1 microM VIP for 15 min. The effect of 1 microM VIP was mimicked by 10 microM dibutyryl cyclic AMP and attenuated by dopamine (1 x 10(-4) M), while colchicine, beta-lumicolchicine, and monensin, all at 1 microM, had no effect.
Koh
S W
SW
Department of Ophthalmology, University of Maryland, Baltimore 21201.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology; D004298:Dopamine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D008162:Lumicolchicines / Q000494:pharmacology; D046911:Macromolecular Substances; D008985:Monensin / Q000494:pharmacology; D010539:Permeability; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D012168:Retinal Pigments / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000502:physiology*
D008162:Lumicolchicines; D046911:Macromolecular Substances; D012168:Retinal Pigments; D014660:Vasoactive Intestinal Peptide; D008985:Monensin; D000242:Cyclic AMP; D003078:Colchicine; D004298:Dopamine