Production of the thiyl free radical by the reaction of N-methyl-N'-nitro-N-nitrosoguanidine with L-cysteine.
The thiyl free radical is formed in the L-cysteine/N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) system (pH 7.8) without exposure to light as detected by the ESR spin trapping technique. The formation of N-methyl-N'-nitroguanidine in the system is identified by thin-layer chromatography. The hypothesis that the thiyl radical is formed by the attack of the nucleophilic reagent L-cysteine on the nitroso group of MNNG is verified by these results.
Mikuni
T
T
Department of Gastrointestinal Oncology, Center for Adult Diseases, Osaka, Japan.
Tatsuta
M
M
D002855:Chromatography, Thin Layer; D003497:Cyclic N-Oxides; D003545:Cysteine / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000737:chemistry*; D008769:Methylnitronitrosoguanidine / Q000737:chemistry*; D013997:Time Factors
D003497:Cyclic N-Oxides; D005609:Free Radicals; D008769:Methylnitronitrosoguanidine; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D003545:Cysteine