Rapid polymerization of LR-white for immunocytochemistry.
London Resin (LR) White a hydrophilic embedding medium for immunocytochemistry, can be polymerized in a commercial microwave oven in seven minutes using the chemical accelerator benzoyl peroxide. In order to minimize the effects of heating, the polymerization vessel was maintained in an ice bath. We demonstrate that this procedure does not affect the antigenicity of either barley aleurone nuclease or of the catalytic and regulatory subunits of rat parotid cAMP-dependent protein kinase.
Hillmer
S
S
Abteilung Cytologie, Universität Göttingen, Federal Republic of Germany.
Joachim
S
S
Robinson
D G
DG
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D003890:Desiccation; D004720:Endonucleases / Q000032:analysis; D001467:Hordeum / Q000201:enzymology; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D010306:Parotid Gland / Q000201:enzymology; D011108:Polymers / Q000138:chemical synthesis; D011494:Protein Kinases / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000180:Acrylic Resins; D011108:Polymers; C048707:LR white; D011494:Protein Kinases; D004720:Endonucleases