Substitution by fatty acids for phosphatidylserine in a reconstitution of phorbol ester binding to protein kinase C.
1. Fatty acids can be substituted or phosphatidylserine in a reconstitution of phorbol ester binding to protein kinase C. 2. Phorbol ester, however, does not seem to be effectively utilized for the activation of the enzyme. 3. It is suggested that fatty acids play a role on the activation of protein kinase C in the abnormal conditions such as ischemia, while the phospholipid-dependent activation has a physiological significance in normal conditions.
Irita
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Glaxo Research Laboratories, Research Triangle Park, NC 27709.
Cuatrecasas
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Hebdon
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D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001921:Brain / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology; D004537:Eicosanoic Acids / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005227:Fatty Acids / Q000008:administration & dosage / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D010703:Phorbol Esters / Q000378:metabolism*; D010718:Phosphatidylserines / Q000494:pharmacology*; D010770:Phosphotransferases / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013229:Stearic Acids / Q000494:pharmacology
D001095:Arachidonic Acids; D004537:Eicosanoic Acids; D005227:Fatty Acids; D009829:Oleic Acids; D010703:Phorbol Esters; D010718:Phosphatidylserines; D013229:Stearic Acids; D016718:Arachidonic Acid; D019301:Oleic Acid; C031183:stearic acid; D010770:Phosphotransferases; D011493:Protein Kinase C; D008274:Magnesium; D002118:Calcium