HPLC and 1H-NMR study of chiral recognition in some thromboxane antagonists induced by beta-cyclodextrin.
The interaction of beta-cyclodextrin with a series of structurally related chiral thromboxane antagonists was investigated using NMR and RP-HPLC. HPLC studies used both a cyclodextrin bonded phase (Cyclobond I), and beta-cyclodextrin as a mobile phase additive with an achiral C8 column. Many of the compounds exhibited chiral recognition with beta-cyclodextrin in each technique, but only partial correlations between the three data sets were observed. HPLC and ROESY NMR data suggested the possibility of bimodal inclusion.
Casy
A F
AF
School of Pharmacy and Pharmacology, University of Bath, Claverton Down, UK.
Cooper
A D
AD
Jefferies
T M
TM
Gaskell
R M
RM
Greatbanks
D
D
Pickford
R
R
D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011522:Protons; D013237:Stereoisomerism; D013931:Thromboxanes / Q000037:antagonists & inhibitors*; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D011522:Protons; D013931:Thromboxanes; D047392:beta-Cyclodextrins; C031215:betadex