title: Retrospective study of myocardial canine parvovirus infection by in situ hybridization.
abstract: The diagnosis of myocardial canine parvovirus (CPV) infection used to depend on the presence of pathognomonic intranuclear inclusion bodies. The in situ hybridization technique, however, allowed to detect CPV specific nucleic acid in myocardial tissue where no inclusion bodies were found. Hence, we applied this technique to check formalin-fixed paraffin-embedded myocardial tissue from puppies with heart lesions for the presence of CPV. The tissues had been collected between 1977 and 1989. A biotinylated probe was used for in situ hybridization. This way CPV specific nucleic acid was detected in 3 dogs where CPV myocarditis had not been diagnosed on routinely stained slides because of the lack of intranuclear inclusion bodies. However, in spite of the application of the in situ hybridization technique no further myocardial CPV infection was detected in puppies with heart lesions from after 1979, confirming that the number of puppies with myocardial CPV infection declined after that year.
authors.affiliation: Institut für Veterinärpathologie, Universität Zürich, Switzerland.
authors.forename: A S
authors.initials: AS
authors.lastname: Waldvogel
authors.forename: S
authors.initials: S
authors.lastname: Hassam
authors.forename: R
authors.initials: R
authors.lastname: Weilenmann
authors.forename: J D
authors.initials: JD
authors.lastname: Tratschin
authors.forename: G
authors.initials: G
authors.lastname: Siegl
authors.forename: T
authors.initials: T
authors.lastname: Hänichen
authors.forename: J
authors.initials: J
authors.lastname: Briner
authors.forename: A
authors.initials: A
authors.lastname: Pospischil
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D009205:Myocarditis / Q000175:diagnosis / Q000662:veterinary*; D009693:Nucleic Acid Hybridization; D010321:Parvoviridae / Q000235:genetics; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D012189:Retrospective Studies
chemical_list: D004279:DNA, Viral