Thrombin stimulates the production of phosphatidylinositol 3,4-bisphosphate in human erythroleukemia cells.
The human erythroleukemic cell line, HEL, which has numerous platelet markers, shows enhanced inositol phosphate production in response to thrombin. We investigated the production of phosphoinositides in HEL cells and showed that thrombin stimulates the turnover of several phosphoinositides including the synthesis of phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2). Phosphatidylinositol 3-monophosphate is also produced in HEL cells and its synthesis is not stimulated by thrombin. Pretreatment of HEL cells with the stable prostacyclin analog iloprost inhibits the thrombin-induced increase in the production of PtdIns(3,4)P2. 3-Phosphorylated phosphoinositides have been implicated in signal transduction and regulation of cell proliferation in other cells and may be involved in signal transduction in HEL cells.
Nolan
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Division of Cell Biology, Burroughs Wellcome Co., Research Triangle Park, North Carolina 27709.
Lapetina
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EG
D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000302:isolation & purification / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology*; D014316:Tritium
D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; C060980:phosphatidylinositol 3,4-diphosphate; D014316:Tritium; D013917:Thrombin