[Cytofluorometric study of myeloperoxidase (MPO) expression in neutrophilic granulocytes of subjects with primary or secondary MPO deficiency: analysis of the histographic antigen distribution using the Kolmogorov-Smirnov mathematical model].
Neutrophil granulocytes from 12 subjects with primitive myeloperoxidase (MPO) deficiency (6 totally deficient) and 16 patients with secondary partial MPO deficiency were tested using two different anti-MPO monoclonal antibodies (MoAbs), in combination with a flow cytometer. Results demonstrated three different patterns of immunoreactivity with the MPO protein:i) a bright MPO antigenic expression, typical of patients with secondary MPO deficiency (comparable with that observed in the control group); ii) a medium MPO antigenic expression, typical of subjects with hereditary partial MPO deficiency; and iii) a dim MPO antigenic expression, characteristic of individuals with hereditary total MPO deficiency. No significant differences in granulocyte MPO reactivity were demonstrated for the two MoAbs. Furthermore, in two individuals with complete primitive deficiency, the single histogram analysis of MPO fluorescence seemed to show that only 38% (case 1) and 44% (case 2) of neutrophil were reactive with the MoAbs anti-MPO: the use of multiple histogram analysis in combination with Kolmogorov-Smirnov statistics allowed us to demonstrate that all the cells express a low density of MPO antigen. These data suggest that patients with primary MPO deficiency have different amount of MPO antigens in the neutrophils, and the levels of MPO fluorescence seem to decline concurrently with enzyme activity, thereby suggesting the presence of a diminished MPO production. On the contrary, in most cases of acquired MPO deficit, the reduced cytochemical activity contrasts with normal antigenic reactivity: this might be the result of the presence of an inactive enzyme.
Lanza
F
F
Istituto di Ematologia dell'Università di Ferrara.
Latorraca
A
A
Ferrari
L
L
Pazzi
I
I
Tallarico
A
A
Gandini
D
D
D000911:Antibodies, Monoclonal; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008962:Models, Theoretical; D009504:Neutrophils / Q000201:enzymology* / Q000473:pathology; D009195:Peroxidase / Q000096:biosynthesis / Q000172:deficiency* / Q000276:immunology
D000911:Antibodies, Monoclonal; D009195:Peroxidase
Studio citofluorimetrico della espressione della mieloperossidasi (MPO) nei granulociti neutrofili dei soggetti con deficit primitivo e secondario di MPO: analisi della distribuzione istografica degli antigeni secondo il modello matematico di Kolmogorov-Smirnov.