Lepidopteran cell variants resistant to 5-bromodeoxyuridine and their use for transfection of the HSV-TK gene.
Following mutagenesis of cultured lepidopteran cells (Spodoptera frugiperda) by ethylmethanesulfonate, three variants resistant to 5-bromodeoxyuridine (BrdUrd) were isolated. These clones were 100- to 200-fold more resistant to BrdUrd than the parental cells and were shown to be deficient in thymidine kinase (TK). The drug-resistant phenotype was stable for up to three years of culture under nonselective conditions. It was also found that the S. frugiperda cell line was highly resistant to aminopterin. A selective medium was formulated and used to select herpes simplex virus thymidine kinase (HSV-TK) transfectants from the TK-deficient cells.
Xie
W D
WD
Research Institute of Entomology, Zhongshan University, Guangzhou, People's Republic of China.
He
D F
DF
Wang
X Z
XZ
Long
Q X
QX
Pu
Z L
ZL
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D004274:DNA, Recombinant; D004351:Drug Resistance; D005814:Genes, Viral; D009036:Moths / Q000166:cytology; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection
D004274:DNA, Recombinant; D013937:Thymidine Kinase; D001973:Bromodeoxyuridine