title: Interferon transiently modulates intranuclear signalling system in erythroleukemia Friend cells.
abstract: The effect of human recombinant DNA interferon alpha type A on nuclear inositol lipids, diacylglycerol (DAG) and DNA metabolism has been investigated in Friend erythroleukemia cells. A transient enhancement of phosphatidylinositol (4,5) - bisphosphate (PIP2) phosphorylation together with an increase of diacylglycerol mass were observed in nuclei isolated from cells treated with interferon alpha for 90 min. At the same time, a marked reduction of DNA polymerase alpha activity was observed, suggesting a possible involvement of nuclear inositol fraction in the response of the cell nucleus to interferon treatment.
authors.affiliation: Istituto di Morfologia Umana Normale, Università di Chieti, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Miscia
authors.forename: A
authors.initials: A
authors.lastname: Cataldi
authors.forename: R
authors.initials: R
authors.lastname: Lisio
authors.forename: G
authors.initials: G
authors.lastname: Tulipano
authors.forename: R
authors.initials: R
authors.lastname: Rizzoli
authors.forename: R
authors.initials: R
authors.lastname: Rana
authors.forename: L
authors.initials: L
authors.lastname: Cocco
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D007370:Interferon Type I / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010716:Phosphatidylinositols / Q000378:metabolism; D011994:Recombinant Proteins; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: C011439:1,2-diacylglycerol; D004075:Diglycerides; D007370:Interferon Type I; D010716:Phosphatidylinositols; D011994:Recombinant Proteins; D004257:DNA Polymerase II