Multiple simian virus 40 enhancer elements mediate the trans-activating function of the X protein of hepatitis B virus.
Transient transfections of tissue culture cells with plasmids encoding the X protein of hepatitis B virus result in a transcriptional trans-activation of certain target genes. Our experiments reveal that several individual simian virus 40 enhancer elements and a control element present in the mouse major histocompatibility class I gene H-2Kb are able to mediate the trans-activating function of the X protein. The data suggest that known cellular transcription factors that bind specifically to the multiple enhancer elements participate in trans-activation.
Luber
B
B
Max-Planck-Institut für Biochemie, Martinsried, FRG.
Bürgelt
E
E
Fromental
C
C
Kanno
M
M
Koch
W
W
D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics*; D015534:Trans-Activators; D014158:Transcription, Genetic
D006514:Hepatitis B Surface Antigens; D012333:RNA, Messenger; D015534:Trans-Activators