Characterization of crystals of an Fab fragment of a murine monoclonal antibody.
The Fab fragment of an antibody, made against an E2-specific feline infectious peritonitis virus neutralizing antibody, has been crystallized in a form suitable for X-ray diffraction analysis from PEG 4000 using vapor diffusion methods. The Fab fragment crystals diffract to about 2.9 A resolution and are of triclinic space group P1. Unit cell dimensions, by which the reciprocal lattice can be indexed, are a = 57.16 A, b = 70.85 A, c = 75.81 A, alpha = 85.11 degrees, beta = 121.28 degrees and gamma = 116.33 degrees. There are two Fab fragments comprising the asymmetric unit of the crystals. The presence of a pseudo-mirror plane in the diffraction pattern suggests the presence of at least an approximate dyad axis relating the two Fab fragments within the asymmetric unit.
Ban
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Department of Biochemistry, University of California, Riverside 92521.
Escobar
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C
Day
J
J
Greenwood
A
A
Larson
S
S
McPherson
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D016765:Coronavirus, Feline / Q000276:immunology*; D003460:Crystallization; D003461:Crystallography; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D051379:Mice
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments