Purification of ferredoxin-NADP+ reductase, flavodoxin and ferredoxin from a single batch of the cyanobacterium Anabaena PCC 7119.
Methods are described for the simultaneous isolation of ferredoxin-NADP+ reductase, ferredoxin and flavodoxin from large quantities of the cyanobacterium Anabaena PCC 7119 allowing the use of a single batch of cells. The ultraviolet-visible spectra and the extinction coefficients of ferredoxin-NADP+ reductase and ferredoxin were determined. The purification procedure also yields enriched fractions of phycobiliproteins and cytochrome c553.
Pueyo
J J
JJ
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Spain.
Gómez-Moreno
C
C
D017033:Anabaena / Q000737:chemistry* / Q000201:enzymology; D002455:Cell Division; D002848:Chromatography, DEAE-Cellulose; D003574:Cytochrome c Group / Q000302:isolation & purification; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry / Q000302:isolation & purification*; D005288:Ferredoxins / Q000737:chemistry / Q000302:isolation & purification*; D005418:Flavodoxin / Q000737:chemistry / Q000302:isolation & purification*; D045342:Light-Harvesting Protein Complexes; D010940:Plant Proteins / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet
D003574:Cytochrome c Group; D005288:Ferredoxins; D005418:Flavodoxin; D045342:Light-Harvesting Protein Complexes; D010940:Plant Proteins; C083951:cytochrome c553; C022302:cytochrome C-552; D005287:Ferredoxin-NADP Reductase