The phagocyte oxidative metabolism function in ankylosing spondylitis.
The phagocyte oxydative metabolism function was measured using chemiluminescence in microamounts of whole blood in 15 ankylosing spondylitis (AS) patients (10 were B27 positive), and in 17 controls. It was obtained from cells at rest, and following stimulation (latex, zymosan, fMLP), with luminol and lucigenin as amplifiers. The maximal light intensity was significantly higher (P less than 0.01) in AS compared to the controls in resting cells as well as in those after stimulation. There was no difference between HLA-B27 positive or negative AS patients. The increase in oxidative metabolism of the phagocyte system in AS was more evident in the luminol dependent assay, suggesting an activation of the myeloperoxydase system.
Wendling
D
D
Service de Rhumatologie, Centre Hospitalier Régional et Universitaire, Besançon, France.
Didier
J M
JM
Vuitton
D A
DA
D000293:Adolescent; D000328:Adult; D006801:Humans; D007840:Latex / Q000494:pharmacology; D008163:Luminescent Measurements; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism; D010586:Phagocytes / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D013167:Spondylitis, Ankylosing / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology*; D015054:Zymosan / Q000494:pharmacology
D007840:Latex; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D009195:Peroxidase