Polymeric genes MEL8, MEL9 and MEL10--new members of alpha-galactosidase gene family in Saccharomyces cerevisiae.
We used a combination of genetic hybridization analysis and electrokaryotyping with radioactively labelled MEL1 gene probe hybridization to isolate and identify seven polymeric genes for the fermentation of melibiose in strain CBS 5378 of Saccharomyces cerevisiae (syn. norbensis). Four of the MEL genes, i.e. MEL3, MEL4, MEL6 and MEL7, were allelic to those found in S. cerevisiae strain CBS 4411 (syn. S. oleaginosus) whereas three genes, i.e. MEL8, MEL9 and MEL10 occupied new loci. Electrokaryotyping showed that all seven MEL genes in CBS 5378 were located on different chromosomes. The new MEL8, MEL9 and MEL10 genes were found on chromosomes XV, X/XIV and XII, respectively.
Naumov
G
G
All-Union Scientific Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, USSR.
Naumova
E
E
Turakainen
H
H
Suominen
P
P
Korhola
M
M
D015139:Blotting, Southern; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005285:Fermentation; D005800:Genes, Fungal; D005838:Genotype; D008553:Melibiose / Q000378:metabolism; D005810:Multigene Family; D010375:Pedigree; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D000519:alpha-Galactosidase / Q000235:genetics*
D004271:DNA, Fungal; D008553:Melibiose; D000519:alpha-Galactosidase