Characterization of the murine pancreatic receptor for gastrin releasing peptide and bombesin.
The murine pancreatic receptor for bombesin and gastrin releasing peptide (GRP) has been characterized. Analysis of the binding of 125I-GRP to membranes indicates a single class of sites (10(-13) mol/mg protein) with Kd of 43 pM. A 70 kDa membrane protein was cross-linked to 125I-GRP by bis(sulfosuccinimidyl) suberate; labeling was blocked by GRP, GRP (14-27), AcGRP(20-27), GRP(18-27), bombesin and ranatensin, was partially blocked by [Leu13 psi (CH2NH)Leu14]bombesin and was unaffected by GRP(21-27) and GRP(1-16). The IC50 values for the competitive displacement of 125I-GRP from intact membranes by these peptides were similar to those obtained by the cross-linking experiments showing that the 70 kDa protein is the GRP receptor. The GRP receptor is G-protein coupled; divalent cations are required for high-affinity binding and nonhydrolyzable GTP analogs decrease receptor affinity. In minced pancreas, GRP caused a dose-dependent increase in inositol phosphates implicating phospholipase C in signal transduction. We suggest that the murine pancreatic receptor for bombesin/GRP is a 70 kDa membrane protein, is associated with a G-protein and stimulates phosphatidylinositol turnover.
Fanger
B O
BO
Merrell Dow Research Institute, Cincinnati, OH 45215-6300.
Wade
A C
AC
Cardin
A D
AD
D000595:Amino Acid Sequence; D000818:Animals; D001839:Bombesin / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D019204:GTP-Binding Proteins / Q000378:metabolism; D019886:Gastrin-Releasing Peptide; D005755:Gastrins / Q000378:metabolism*; D007294:Inositol / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011122:Polyphosphates / Q000494:pharmacology; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction; D010738:Type C Phospholipases / Q000378:metabolism
D004075:Diglycerides; D005755:Gastrins; D010455:Peptides; D011122:Polyphosphates; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter; D007294:Inositol; D019886:Gastrin-Releasing Peptide; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins; C005692:triphosphoric acid; D001839:Bombesin