Rapid and sensitive quantitation of cyanide in blood and its application to fire victims.
We developed a head-space method for the determination of blood cyanide by gas chromatography with electron-capture detection. In this technique, a reaction precolumn packed with chloramine-T was used for the conversion of hydrogen cyanide into cyanogen chloride. Since the reaction precolumn eliminated the necessity of trapping hydrogen cyanide from biological samples, blood cyanide could be analyzed quickly by acidification only. Using this method, blood cyanide levels of fire victims were determined at autopsy. The serum values of cyanide ranged from 0.11 micrograms/ml to 18.12 micrograms/ml. However, a significantly higher cyanide content was detected in the left ventricular blood than in the right. This indicates that death was caused by the fire and suggests that the collecting point of the blood sample is an important factor in the determination of inhaled cyanide. There was a positive correlation between blood cyanide and carboxyhemoglobin contents.
Shiono
H
H
Department of Legal Medicine, Shimane Medical University, Izumo, Japan.
Maseda
C
C
Akane
A
A
Matsubara
K
K
D001800:Blood Specimen Collection; D002263:Carboxyhemoglobin / Q000032:analysis; D002552:Cerebral Ventricles; D002849:Chromatography, Gas; D003486:Cyanides / Q000032:analysis; D005390:Fires; D006801:Humans; D006856:Hydrogen Cyanide / Q000097:blood*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results
D003486:Cyanides; D006856:Hydrogen Cyanide; C014430:cyanogen chloride; D002263:Carboxyhemoglobin