title: Two screening methods for detection of specific IgE to inhalant allergens. Comparison with skin prick test and RAST.
abstract: We analyzed the technical features of two new screening methods for detection of specific IgE to inhalant allergens. Moreover, their diagnostic accuracy was evaluated comparing the results obtained from the procedures with those from RAST and skin prick test (SPT) in a group of 30 symptomatic allergic patients. The procedures employ a classic ELISA method, where the presence of IgE is revealed by a colored reaction. These two assays appeared to be quick, inexpensive and easy to use. The results obtained showed significant correlations with both RAST and SPT, in particular for Dermatophagoides pteronyssinus, Parietaria officinalis and grasses, which are the allergens chiefly responsible for respiratory atopy in our area.
authors.affiliation: Allergy Centre, DI.M.I., Genoa University Medical School, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Scordamaglia
authors.forename: G
authors.initials: G
authors.lastname: Passalacqua
authors.forename: S
authors.initials: S
authors.lastname: Ruffoni
authors.forename: M N
authors.initials: MN
authors.lastname: Parodi
authors.forename: G
authors.initials: G
authors.lastname: Ciprandi
authors.forename: G W
authors.initials: GW
authors.lastname: Canonica
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D000918:Antibody Specificity; D005069:Evaluation Studies as Topic; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis* / Q000097:blood / Q000276:immunology; D011852:Radioallergosorbent Test; D012882:Skin Tests
chemical_list: D000485:Allergens; D007073:Immunoglobulin E