Hematologic values of New Zealand white rabbits determined by automated flow cytometry.
Hematologic values of peripheral blood from normal adult New Zealand White rabbits were determined by five different automated flow cytometers in use in a routine clinical hematology laboratory: Technicon H1, Coulter Counter CC540, Coulter Counter VCS, Sysmex NE8000 and Sysmex R1000. The software designed for human blood analysis was used in all instances without adaptation. The total numbers of white blood cells, red blood cells, reticulocytes and platelets were measured with high precision and accuracy. Except for hemoglobin content, concordance was excellent for all measured and calculated values among the different automated flow cytometers. Determining the white blood cell differential count was more complex. Eosinophils and lymphocytes were quantified reliably by all the automated flow cytometers used. However, the results were rejected by Technicon H1 and Sysmex NE8000 in 50% of the cases. Rabbit basophils were recognized with accuracy by Technicon H1 only. The proportion of polymorphonuclear versus mononuclear white cells was identical when measured with Technicon H1 and Coulter Counter VCS. These results show that the new generation of automated flow cytometers designed for human blood can be used with some limitations for animal studies. They allow the standardization of normal values and comparison of results among or between laboratories. They also introduce new parameters, the value of which is as yet undefined.
Kabata
J
J
Department of Research, Kantonsspital Basel, Switzerland.
Gratwohl
A
A
Tichelli
A
A
John
L
L
Speck
B
B
D000818:Animals; D001772:Blood Cell Count / Q000295:instrumentation / Q000662:veterinary*; D004906:Erythrocyte Count; D005069:Evaluation Studies as Topic; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation / Q000662:veterinary*; D007958:Leukocyte Count; D008297:Male; D010976:Platelet Count; D011817:Rabbits / Q000097:blood*; D012016:Reference Values; D012156:Reticulocytes