Characterization of neuropeptide Y (NPY) receptors in human hippocampus.
We identified a 50 kDa neuropeptide Y (NPY) receptor from human hippocampus by affinity labeling. NPY specific binding and labeling of the receptor were inhibited in parallel by increasing concentrations of unlabeled NPY (IC50 = 0.27 nM and 0.18 nM, respectively). Peptide YY (PYY), but none of the pancreatic polypeptides, was as effective as NPY in displacing [125I]NPY. NPY fragments inhibited binding with the rank order of potency: NPY greater than NPY13-36 greater than NPY20-36 greater than or equal to NPY18-36 greater than NPY1-36 free acid greater than or equal to NPY26-36. These results demonstrate that the human hippocampal NPY receptor is a 50 kDa protein fitting the classification of a Y2 receptor subtype.
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Department of Pharmacology, University of Nebraska Medical Center, Omaha 68198-6260.
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D000345:Affinity Labels; D000368:Aged; D001345:Autoradiography; D001667:Binding, Competitive; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008297:Male; D008970:Molecular Weight; D019894:Peptide YY; D010455:Peptides / Q000378:metabolism; D017476:Receptors, Neuropeptide Y; D017981:Receptors, Neurotransmitter / Q000378:metabolism*
D000345:Affinity Labels; D003432:Cross-Linking Reagents; D010455:Peptides; D017476:Receptors, Neuropeptide Y; D017981:Receptors, Neurotransmitter; D019894:Peptide YY