Effect of nitric oxide on L-[3H]glutamate binding to rat brain synaptic membranes.
Nitric oxide (NO), which is spontaneously generated from sodium nitroprusside, was shown to inhibit L-[3H]glutamate binding to rat brain synaptic membranes in a concentration-dependent manner. The L-glutamate binding inhibited by NO, was largely recoverable by the addition of hemoglobin, a scavenger of NO, to the assay medium. These results suggest that NO may play an important role in the modulation of excitatory neurotransmission through direct interaction of L-glutamate binding to its physiological synaptic membrane receptors.
Fujimori
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Department of Analytical Chemistry in Hygiene, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Pan-Hou
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D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D006454:Hemoglobins / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000502:physiology; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000378:metabolism*; D013570:Synaptic Membranes / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium
D005971:Glutamates; D006454:Hemoglobins; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D014316:Tritium; D009599:Nitroprusside; D009569:Nitric Oxide; D016202:N-Methylaspartate