The sensitivity of insulin-stimulated and basal Na efflux to ouabain in frog skeletal muscle cells.
1. The sensitivity of Na efflux to ouabain in frog skeletal muscle cells was studied in the presence and absence of insulin. 2. Insulin increased the ouabain-sensitive Na efflux, about two-fold, without any significant effect on the ouabain-insensitive Na efflux; i.e. all components of the Na efflux increased by insulin can be blocked by ouabain. 3. There was no significant difference between the time course of the inhibitory action of ouabain on Na efflux in the presence and absence of insulin: i.e. the binding affinity of the insulin-stimulated Na/K pump to ouabain is same as that of the control.
Marunaka
Y
Y
Division of Respiratory Research, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada.
D000818:Animals; D001834:Body Water / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D009132:Muscles / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology*; D011892:Rana catesbeiana; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects
D007328:Insulin; D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase