Investigation of Shiga-like toxin binding to chemically synthesized oligosaccharide sequences.
Shiga-like toxin (SLT)-I and SLT-II/IIc bound to Synsorbs containing synthetic alpha Gal(1-4)beta Gal (P1 disaccharide), alpha Gal(1-4)beta GlcNAc (P1 trisaccharide), or alpha Gal(1-4)beta Gal(1-4)beta Glc (Pk trisaccharide) sequences but not to Synsorbs containing alpha Gal(1-3)beta Gal, alpha Gal(1-3)beta Gal(1-4)beta GlcNAc, or the hydrophobic oligosaccharide linkage arm. SLT-I had a preference for Synsorbs containing trisaccharides, whereas SLT-II/IIc binding was less selective. 125I-labeled SLT-I remained bound to Pk trisaccharide Synsorb in the presence of lactose, galactose, or EDTA but was partially released by acetic acid, guanidine HCl, or a 10% solution of SDS. Vero cells coincubated with Pk trisaccharide Synsorb and SLT I extract were protected from this toxin, whereas Pk trisaccharide Synsorb was much less efficient at neutralizing SLT-II/IIc activity in Vero cell coincubation experiments. The SLT-IIc component was not responsible for the inefficient neutralization. Results suggest that synthetic oligosaccharide sequences related to the P blood group antigens coupled to inert matrices could be useful for rapid diagnosis or possibly therapeutic intervention in enterohemorrhagic Escherichia coli infections.
Armstrong
G D
GD
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Fodor
E
E
Vanmaele
R
R
D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D003603:Cytotoxins / Q000378:metabolism; D004768:Enterotoxins / Q000378:metabolism*; D004926:Escherichia coli; D006017:Glycolipids; D009113:Muramidase / Q000494:pharmacology; D009500:Neutralization Tests; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000378:metabolism*; D011112:Polymyxin B / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000187:drug effects; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D014709:Vero Cells
D001427:Bacterial Toxins; D003603:Cytotoxins; D004768:Enterotoxins; D006017:Glycolipids; D009844:Oligosaccharides; D011956:Receptors, Cell Surface; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; C068556:Shiga-like toxin receptor; D009113:Muramidase; D011112:Polymyxin B