[Protein adsorption at the tips from synthetic polymers as the cause of incorrect titer analysis of interferons in antiviral bioassays].
The determination of the biological activity of interferons are carried out on the basis of the inhibition of the cytopathic effect of vesicular stomatitis virus on MDBK cells. Using microtiter-plates and tips made of synthetic polymers incorrect data may be observed due to the adsorption of the protein interferon to the tips and, thus, carryover of activity. The following recommendations may help to avoid this kind of mistakes: 1. Generally changing of the tips, at least for the highest concentrations. 2. Reduction of high initial activities by an appropriate pre-dilution. 3. Addition of the detergent Triton X 100 in concentrations between 50 to 100 mg/l to the dilution media for interferon.
Tonew
M
M
Zentralinstitut für Mikrobiologie und experimentelle Therapie Jena.
Ohme
E
E
Gerlach
D
D
Wondraczek
R H
RH
D000327:Adsorption; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002460:Cell Line; D003902:Detergents; D007370:Interferon Type I / Q000032:analysis* / Q000494:pharmacology; D017830:Octoxynol; D011092:Polyethylene Glycols; D011108:Polymers; D011994:Recombinant Proteins; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000502:physiology; D014773:Virology / Q000379:methods
D003902:Detergents; D007370:Interferon Type I; D011108:Polymers; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol
Proteinadsorption an Tips aus synthetischen Polymeren als Ursache für fehlerhafte Titerbestimmungen von Interferonen im antiviralen Bioassay.