Flow injection system based on the sandwich technique for saving expensive reagents.
We report the application of a sandwich technique in flow injection systems which afford low consumption of expensive reagents and two reagent recirculation systems. The potential applicability of the technique thus developed was assessed by determining glucose in serum samples by the enzymatic glucosidase/peroxidase method. It was possible to perform up to 450 determinations with the same amount of reagent used to perform 50 determinations by batch procedures. The sampling rate was 80 determinations per hour with a 0.9% relative standard deviation.
Araujo
A N
AN
Departamento de Química Física, Faculdade de Farmacia, Porto, Portugal.
Lima
J L
JL
Alonso-Chamarro
J
J
Bartrolí
J
J
Poch
M
M
D001786:Blood Glucose / Q000032:analysis*; D002138:Calibration; D003362:Cost-Benefit Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D005959:Glucosidases; D006801:Humans; D007202:Indicators and Reagents; D009195:Peroxidase
D001786:Blood Glucose; D007202:Indicators and Reagents; D009195:Peroxidase; D005959:Glucosidases