title: Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast.
abstract: We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain.
authors.affiliation: School of Botany, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: M B
authors.initials: MB
authors.lastname: Singh
authors.forename: T
authors.initials: T
authors.lastname: Hough
authors.forename: P
authors.initials: P
authors.lastname: Theerakulpisut
authors.forename: A
authors.initials: A
authors.lastname: Avjioglu
authors.forename: S
authors.initials: S
authors.lastname: Davies
authors.forename: P M
authors.initials: PM
authors.lastname: Smith
authors.forename: P
authors.initials: P
authors.lastname: Taylor
authors.forename: R J
authors.initials: RJ
authors.lastname: Simpson
authors.forename: L D
authors.initials: LD
authors.lastname: Ward
authors.forename: J
authors.initials: J
authors.lastname: McCluskey
mesh_terms: D000485:Allergens; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D008129:Lolium / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology; D011058:Pollen / Q000032:analysis* / Q000276:immunology* / Q000648:ultrastructure; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; C042814:Lol p I protein, Lolium perenne; D010940:Plant Proteins; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA