A sequence at the inside end of IS50 down regulates transposition.
Deletion analysis has shown that the segment at the IS50 inside (I) end that is needed for efficient transposition is approximately 19 bp long. Dam methylation at two 5' GATC sequences within this segment decreases I-end transposition activity. A third 5' GATC sequence is present at bp 21-24 of the I end. The comparisons presented here show that extension of the I end from 19 to 24 bp decreases its transposition activity in dam cells 5- to 50-fold, depending on the overall transposon structure.
Dodson
K W
KW
Washington University Medical School, Department of Molecular Microbiology, St. Louis, Missouri 63110-1093.
Berg
D E
DE
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D019895:Transposases
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D008780:Methyltransferases; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C499294:dam protein, E coli; D009713:Nucleotidyltransferases; D019895:Transposases