Role of Na+/H+ exchanger in insulin-stimulated glucose uptake into skeletal muscle.
Insulin-stimulated 2-deoxy-D-glucose uptake by the rat soleus muscle was concluded to be closely related to Na+/H+ exchanger from the following findings; (1) Insulin stimulated Na+ uptake by rat soleus muscle, (2) Amiloride inhibited the effect of insulin on 2-deoxy-D-glucose uptake by the muscle. (3) External Na+ or Li+ was essential for the action of insulin, and Na+ could not be replaced by other monovalent cations such as K+, Rb+, and choline+. The decreases in the effect of insulin on reduction of the pH of the medium, addition of amiloride, or solution for replacement of Na(+)-Hanks buffer by choline(+)-Hanks buffer could not be explained by decrease in specific binding of insulin.
Yamauchi
T
T
2nd Department of Medical Biochemistry, School of Medicine, Ehime University, Japan.
Sekiya
K
K
Okuda
H
H
Kimura
S
S
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000502:physiology*; D009132:Muscles / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D011522:Protons; D051381:Rats; D011919:Rats, Inbred Strains; D015222:Sodium Channels / Q000502:physiology*
D007328:Insulin; D011522:Protons; D015222:Sodium Channels; D010042:Ouabain; D000584:Amiloride; D005947:Glucose