title: The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme.
abstract: The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Distinct versions of the N-end rule operate in all organisms examined, from mammals to bacteria. We show that UBC2(RAD6), one of at least seven ubiquitin-conjugating enzymes in the yeast Saccharomyces cerevisiae, is essential for multiubiquitination and degradation of the N-end rule substrates. We also show that UBC2 is physically associated with UBR1, the recognition component of the N-end rule pathway. These results indicate that some of the UBC2 functions, which include DNA repair, induced mutagenesis, sporulation, and regulation of retrotransposition, are mediated by protein degradation via the N-end rule pathway.
authors.affiliation: Department of Biology, Massachusettes Institute of Technology, Cambridge 02139.
authors.forename: R J
authors.initials: RJ
authors.lastname: Dohmen
authors.forename: K
authors.initials: K
authors.lastname: Madura
authors.forename: B
authors.initials: B
authors.lastname: Bartel
authors.forename: A
authors.initials: A
authors.lastname: Varshavsky
mesh_terms: D004260:DNA Repair; D005656:Fungal Proteins / Q000378:metabolism; D006238:Haploidy; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D044763:Ubiquitin-Conjugating Enzymes; D014452:Ubiquitins / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014452:Ubiquitins; C471606:RAD6 protein, S cerevisiae; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases