Evaluation of the mutagenic effects of SV40 in mouse, hamster, and mouse-human hybrid cells.
We have examined the ability of SV40 to induce changes in drug or temperature resistance in mouse, hamster, and mouse-human hybrid cells. SV40 induced a substantial increase of cells resistant to 5-bromodeoxyuridine + trifluorothymidine in Balb/c 3T3 cells and induced an increase of hybrid cells resistant to 6-thioguanine. SV40 was found to be nonmutagenic or weakly mutagenic in other test systems. The 3T3 cells were T-antigen positive, exhibited a marked reduction in TK activity, were heterogeneous for [3H]BrdU incorporation by autoradiography, and exhibited instability of the drug-resistance phenotype, suggesting that SV40 may be inducing resistance by an epigenetic process. SV40-induced 6-thioguanine resistance in the hybrids appears to occur predominantly by chromosome loss.
Giles
R E
RE
Department of Microbiology, University of Michigan Medical School, Ann Arbor 48109.
Boyce
F M
FM
Brockman
W W
WW
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000032:analysis; D001973:Bromodeoxyuridine / Q000378:metabolism; D002461:Cell Line, Transformed / Q000473:pathology; D006224:Cricetinae; D003412:Cricetulus; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D007739:L Cells / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016296:Mutagenesis; D010053:Ovary / Q000473:pathology*; D010641:Phenotype; D013539:Simian virus 40 / Q000235:genetics*; D013696:Temperature; D013866:Thioguanine / Q000494:pharmacology; D013936:Thymidine / Q000031:analogs & derivatives / Q000494:pharmacology; D013937:Thymidine Kinase / Q000378:metabolism; D014412:Tumor Virus Infections / Q000235:genetics / Q000473:pathology
D000957:Antigens, Viral, Tumor; D013937:Thymidine Kinase; D013866:Thioguanine; D001973:Bromodeoxyuridine; D013936:Thymidine