Which 3-ribofuranosyl-substituted purine 5'-phosphates undergo template-directed oligomerization?
We have studied the oligomerization reactions of the 2-methylimidazolide derivatives of 3-isoisoguanosine 5'-phosphate (2) and 3-isoxanthosine 5'-phosphate (5) in the presence of a variety of homopolynucleotide templates. In no case did we observe a substantial template-facilitated production of long oligomers. Polyuridylic acid directed the synthesis of low molecular-weight products from both monomers. Polycytidylic acid, polyadenylic acid, polyinosinic acid, and polyguanylic acid were ineffective as templates in the systems that we investigated.
Hill
A R
AR
Salk Institute for Biological Studies, San Diego, CA 92186-5800.
Kumar
S
S
Patil
V D
VD
Leonard
N J
NJ
Orgel
L E
LE
D002851:Chromatography, High Pressure Liquid; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives* / Q000235:genetics; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives / Q000138:chemical synthesis; D012263:Ribonucleosides / Q000138:chemical synthesis; D013698:Templates, Genetic
D012263:Ribonucleosides; C070260:3-isoisoguanosine 5'-phospho-2-methylimidazole; C070259:3-ribofuranosylisoguanosine 5'-diphosphate; C070261:3-isoxanthosine 5'-phospho-2-methylimidazole; D006153:Guanosine Diphosphate; D006157:Guanosine Monophosphate