title: The effect of copper deficiency on adriamycin toxicity.
abstract: The effect of copper (Cu) deficiency on antioxidant status of liver and heart and its effect on adriamycin toxicity was assessed. Weanling rats were fed Cu-adequate (+Cu) and Cu-deficient (-Cu) diets for 3 weeks and then injected intraperitoneally once with saline or adriamycin (8 mg/kg). All -Cu rats had increased heart/body weight ratios compared to +Cu rats. Regardless of Cu status, adriamycin decreased body weight and increased heart cytochrome c oxidase, but had no effect on Cu,Zn-superoxide dismutase, glutathione peroxidase, the thiobarbituric acid (TBA) index or liver cytochrome c oxidase activity. -Cu, adriamycin rats had significantly decreased heart weights (0.54 + 0.02 g/kg body wt.) compared to -Cu, saline rats (0.69 +/- 0.05 g/kg body wt.). Four of 6 -Cu rats given adriamycin had abnormal EKGs, with 3 showing extrasystoles and 1 having a severe bradycardia. All +Cu rats given adriamycin had normal EKGs. Pathological changes detected with electron microscopy were most severe in -Cu rats given adriamycin.
authors.affiliation: Department of Foods and Nutrition, University of Georgia, Athens.
authors.forename: J
authors.initials: J
authors.lastname: Fischer
authors.forename: M A
authors.initials: MA
authors.lastname: Johnson
authors.forename: R L
authors.initials: RL
authors.lastname: Tackett
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003300:Copper / Q000172:deficiency*; D004317:Doxorubicin / Q000633:toxicity*; D003576:Electron Transport Complex IV / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000648:ultrastructure; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D003300:Copper; D004317:Doxorubicin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003576:Electron Transport Complex IV