title: The mitochondrial genome of Schizosaccharomyces pombe. Stimulation of intra-chromosomal recombination in Escherichia coli by the gene product of the first cox1 intron.
abstract: The open reading frame of the first intron of the mitochondrial cox1 gene (cox1I1) was expressed in Escherichia coli. The putative intron-encoded protein stimulated the formation of intra-chromosomal lac(+)-recombinants about threefold. No stimulation was found when the reading frame was inserted in the opposite direction, or when it was interrupted by a deletion. The intronic open reading frame did not complement recA- or recB- mutants of E. coli. In S. pombe, elimination of this intron did not abolish homologous recombination in mitochondria. A possible role of the recombinase activity in yeast mitochondria will be discussed.
authors.affiliation: Istituto Internazionale di Genetica e Biofisica, CNR, Napoli, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Manna
authors.forename: D R
authors.initials: DR
authors.lastname: Massardo
authors.forename: L
authors.initials: L
authors.lastname: Del Giudice
authors.forename: A
authors.initials: A
authors.lastname: Buonocore
authors.forename: A G
authors.initials: AG
authors.lastname: Nappo
authors.forename: P
authors.initials: P
authors.lastname: Alifano
authors.forename: B
authors.initials: B
authors.lastname: Schäfer
authors.forename: K
authors.initials: K
authors.lastname: Wolf
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D004722:Endoribonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D019426:Integrases; D007438:Introns; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D016366:Open Reading Frames; D012326:RNA Splicing; D045522:Recombinases; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D045522:Recombinases; D003576:Electron Transport Complex IV; D004254:DNA Nucleotidyltransferases; D019426:Integrases; D009713:Nucleotidyltransferases; C115006:integron integrase IntI1; C034038:mRNA maturase; D004722:Endoribonucleases