Avian tibial dyschondroplasia: a comparison of the incidence and severity as assessed by gross examination and histopathology.
The assessment of avian tibial dyschondroplasia (TD) in many recent studies is based on examination of slices of the proximal tibiotarsus with the naked eye. This study examines the incidence and severity of TD in broilers under four different dietary regimes and compares the efficacy of naked eye assessment with histopathological examination. The diets contained reduced levels of calcium relative to phosphorus with adequate (diet 1) and high (diet 2) levels of vitamin D3 supplementation; a very low calcium diet (diet 3) and a standard diet (diet 4) were also included. Gross examination suggested that TD was present in 80 per cent, 79 per cent and 27 per cent of tibiotarsi from birds on diets 1, 2 and 4, respectively. However, histological examination indicated TD, correspondingly, to be present in 18 per cent, 39 per cent and 6 per cent of tibiotarsi. Some birds on diet 1 exhibited physeal changes consistent with mild hypocalcaemic rickets. Many of the bone extremities which, grossly, were considered dyschondroplastic (diets 1, 2 and 4), histologically were shown to have an extensive hypertrophied zone with poor metaphyseal vessel penetration, but no cellular or matrix changes consistent with TD. This cast doubt on the validity of earlier studies of TD which relied solely on naked eye examination. The birds on diet 3 all demonstrated histopathology considered consistent with severe hypocalcaemic rickets and the present study suggests that in three-week-old broilers a moderately hypocalcaemic diet with a high level of vitamin D3 will not cause rickets but will increase the incidence and severity of TD.(ABSTRACT TRUNCATED AT 250 WORDS)
Thorp
B H
BH
Institute of Animal Physiology and Genetics, Edinburgh Research Station, Roslin, Midlothian.
Whitehead
C C
CC
Rennie
J S
JS
D000818:Animals; D002136:Calcium, Dietary / Q000008:administration & dosage; D002645:Chickens; D002762:Cholecalciferol / Q000008:administration & dosage; D004032:Diet; D015994:Incidence; D010009:Osteochondrodysplasias / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D016226:Phosphorus, Dietary / Q000008:administration & dosage; D011201:Poultry Diseases / Q000209:etiology / Q000473:pathology*; D013977:Tibia / Q000473:pathology*
D002136:Calcium, Dietary; D016226:Phosphorus, Dietary; D002762:Cholecalciferol