Inhibition of B6RV2 leukemia growth by immunization with purified unique antigen.
Monoclonal antibody (mAb) NU7-99 reacted with only B6RV2 cells, not with 28 other leukemia cell lines, fibroblasts or normal tissues. Biochemical analyses of the unique antigen on B6RV2 cells that reacted with NU7-99 mAb indicated its relationship to xenotropic murine leukemia virus gp70. The antigen that reacted with NU7-99 mAb was extracted from the surface of B6RV2 cells with n-butanol and purified by ion-exchange chromatography and affinity chromatography. Growth of B6RV2 tumors in semi-syngeneic mice was inhibited by immunization of the mice with a purified preparation of this unique antigen.
Uenaka
A
A
Department of Tumor Immunology, Center for Adult Diseases, Osaka.
Nakayama
E
E
D020001:1-Butanol; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000302:isolation & purification; D000440:Butanols / Q000737:chemistry; D007114:Immunization; D007942:Leukemia, Experimental / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D051379:Mice
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D000440:Butanols; D020001:1-Butanol