Fructose-2,6-bisphosphate metabolism in permeabilized yeast cells.
Several methods for the permeabilization of Saccharomyces cerevisiae M1 were compared. Cells were permeabilized in the presence of 3% toluene/mercaptoethanol, and the activities of 6-phosphofructo-2-kinase, fructose-2,6-bisphosphatase and alkaline phosphatase were measured during growth of yeast on glucose. In the exponential phase of growth, the specific activities of 6-phosphofructo-2-kinase and fructose-2,6-bisphosphatase decrease significantly. The specific activities of 6-phosphofructo-2-kinase and high-affinity fructose-2,6-bisphosphatase increase again during the transition phase and reach maximum values in the stationary phase. In contrast to the specific activities, the activity concentrations of 6-phosphofructo-2-kinase and fructose-2,6-bisphosphatase remain nearly constant in the exponential phase, but increase in the transition and the stationary growth phase. The concentration of fructose-2,6-bisphosphate drops from about 6 microM in the exponential phase to very low levels in the transition phase, but increases slightly in the stationary phase. In Saccharomyces cerevisiae M1 several fructose-2,6-bisphosphate degrading activities were measured differing in the behaviour during growth on glucose, in the pH-optimum and the inhibition by fructose-6-phosphate.
Kessler
R
R
Institut für Biochemie, Bereich Medizin, Universität Leipzig, BRD.
Grtner
G
G
Schellenberger
W
W
Hofmann
E
E
D000469:Alkaline Phosphatase / Q000378:metabolism; D002463:Cell Membrane Permeability; D003470:Culture Media; D005635:Fructosediphosphates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008623:Mercaptoethanol; D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D014050:Toluene
D003470:Culture Media; D005635:Fructosediphosphates; D014050:Toluene; D008623:Mercaptoethanol; C027652:fructose 2,6-diphosphate; D010770:Phosphotransferases; D025481:Phosphofructokinase-2; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D005947:Glucose