[Tumor necrosis factor in medicinal interferon preparations].
In response to virus induction a culture of donor leukocytes alongside with interferon (IF-alpha) produced a factor of tumor necrosis (TNF). The kinetics of TNF and IF-alpha biosynthesis did not depend on the kind of IF used for priming, was rapid, with maximum production within 7-8 hours. Antibodies to IF-alpha and IF-alpha had no effect on TNF production, while antibody to TNF did not reduce IF-alpha yields. TNF in detectable titres was present in medical preparations of native IF-alpha but was absent in preparations of recombinant IF-alpha and IF-alpha as well as in an injection preparation of IF purified by chemical methods.
Kuznetsov
V P
VP
Babaiants
A A
AA
Frolova
I S
IS
Zueva
L A
LA
Beliaev
D L
DL
Khusainov
R M
RM
D'iakonova
E V
EV
Vyborov
A M
AM
Parfenov
V V
VV
D000906:Antibodies / Q000276:immunology; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004340:Drug Contamination; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D007370:Interferon Type I / Q000032:analysis; D016898:Interferon-alpha / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D009522:Newcastle disease virus; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000472:pathogenicity
D000906:Antibodies; D007369:Interferon Inducers; D007370:Interferon Type I; D016898:Interferon-alpha; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha
Faktor nekroza opukholi v meditsinskikh preparatakh interferona.