Effects of interleukin-1 and gamma interferon on total intracellular magnesium concentration in a murine lymphoid cell line.
Treatment of the pre-B lymphoid cell line 70Z/3 by interleukin-1 alpha (IL-1 alpha) and gamma-interferon (IFN-gamma) induces kappa-immunoglobulin light chain gene expression resulting in surface IgM expression. In this study we examined changes in total intracellular magnesium concentration [Mg]i, in 70Z/3 cells following lymphokine stimulation. After 30 min of treatment, [Mg]i increased by delta [Mg]i = 2.8 +/- 1.1 mM (n = 10, p < 0.025) with IL-1 alpha, and by delta [Mg]i = 3.0 +/- 1.6 mM (n = 8, p < 0.05) with IFN-gamma. After 4 h of treatment, the effect of IL-1 alpha was sustained, delta [Mg]i = 4.0 +/- 0.8 mM (n = 4, p < 0.01), whereas that of IFN-gamma diminished, delta [Mg]i = 0.2 +/- 0.6 mM (n = 7). These cytokines did not induce measurable changes in cell water volume indicating that the lymphokine-induced increase in [Mg]i represented Mg2+ influx.
Huynh
T T
TT
Department of Medicine, University of Washington School of Medicine, Seattle.
Bomsztyk
K
K
D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry* / Q000187:drug effects; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000737:chemistry* / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D008274:Magnesium / Q000032:analysis*; D051379:Mice; D013997:Time Factors
D007375:Interleukin-1; D007371:Interferon-gamma; D008274:Magnesium