Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake.
Two Corynebacterium glutamicum mutants defective in lysine uptake were identified by analysing mutants resistant to S-(2-aminoethyl)-cysteine (AEC). A 5.6 kb genomic DNA fragment restoring AEC sensitivity and lysine uptake was isolated. A 4.2 kb subfragment was sequenced and three open reading frames were identified. Subcloning and gene disruption experiments showed that only the first open reading frame, termed lysl, is involved in lysine uptake. Lysl consists of 501 amino acids with a Mr of 53600. The hydrophobicity profile suggests that the lysl gene product is an integral membrane protein with 13 transmembrane segments. The amino acid sequence of lysl displays strong homology to that of the arcD gene product of Pseudomonas aeruginosa, which is proposed to act as an arginine-ornithine antiporter. Investigation of the influence of the lysl gene on lysine secretion suggests the existence of a separate lysine efflux system in C. glutamicum.
Seep-Feldhaus
A H
AH
Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Germany.
Kalinowski
J
J
Pühler
A
A
D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems; D026923:Amino Acid Transport Systems, Basic; D017920:Antiporters; D001120:Arginine / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics*; D005816:Genetic Complementation Test; D006801:Humans; D007231:Infant, Newborn; D008239:Lysine / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009952:Ornithine / Q000378:metabolism; D011487:Protein Conformation; D016364:Reading Frames; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D026905:Amino Acid Transport Systems; D026923:Amino Acid Transport Systems, Basic; D017920:Antiporters; C063930:ArcD protein, Bacteria; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; C074173:lysI protein, Corynebacterium glutamicum; D001120:Arginine; D009952:Ornithine; D008239:Lysine