Long-term in vivo expression of genes introduced by retrovirus-mediated transfer into mammary epithelial cells.
Nonimmortalized mouse mammary epithelial cells expressing Escherichia coli beta-galactosidase from a murine amphotropic packaged retroviral vector were injected into the epithelium-divested mammary fat pads of syngeneic mice. Mammary glands formed from the injected mammary epithelial cells contained ductal and lobular cells, both of which expressed beta-galactosidase when examined in situ more than 12 months later. These results indicate that stable recombinant gene expression can be achieved in vivo in the mammary gland without altering the growth properties of normal mammary epithelium.
Smith
G H
GH
Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, Maryland 20892.
Gallahan
D
D
Zwiebel
J A
JA
Freeman
S M
SM
Bassin
R H
RH
Callahan
R
R
D016475:3T3 Cells; D000273:Adipose Tissue / Q000166:cytology / Q000502:physiology; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D004848:Epithelium / Q000502:physiology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005260:Female; D015870:Gene Expression; D009052:Leukemia Virus, Murine / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000382:microbiology / Q000502:physiology*; D051379:Mice; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
D001616:beta-Galactosidase