c-mos proto-oncogene product is partly degraded after release from meiotic arrest and persists during interphase in mouse zygotes.
Recently, it has been shown that the product of the c-mos proto-oncogene is a component of cytostatic factor, an activity present in unfertilized eggs from vertebrates that arrests the cell cycle in metaphase of the second meiotic division (metaphase II) possibly by stabilizing maturation-promoting factor (MPF). We have studied the behavior of the c-mos product in metaphase II mouse oocytes and soon after activation. The amount of c-mos in the oocyte was still very high after second polar body extrusion, when cyclin B has been degraded and MPF activity had decreased dramatically. Degradation of c-mos takes place later, during the G1 phase of the first cell cycle and a residual amount of c-mos is detectable during the first zygotic interphase. Our data show that the degradation of c-mos is not involved in the release from the metaphase arrest.
Weber
M
M
Laboratoire de Physiologie du Développement, Université Paris VII, France.
Kubiak
J Z
JZ
Arlinghaus
R B
RB
Pines
J
J
Maro
B
B
D000818:Animals; D016213:Cyclins / Q000378:metabolism; D005260:Female; D015151:Immunoblotting; D007399:Interphase; D008540:Meiosis; D000090204:Mesothelin; D008677:Metaphase; D051379:Mice; D009865:Oocytes / Q000378:metabolism; D006656:Protamine Kinase / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D017231:Proto-Oncogene Proteins c-mos; D015053:Zygote / Q000378:metabolism*
D016213:Cyclins; C000715528:Msln protein, mouse; D011518:Proto-Oncogene Proteins; D006656:Protamine Kinase; D017231:Proto-Oncogene Proteins c-mos; D000090204:Mesothelin