title: NMR studies of the active site of isopenicillin N synthase, a non-heme iron(II) enzyme.
abstract: The active site structure of isopenicillin N synthase (IPNS) has been previously studied by the use of Mössbauer, EPR, electronic absorption, and NMR spectroscopies [Chen, V.J., Frolik, C.A., Orville, A.M., Harpel, M.R., Lipscomb, J.D., Surerus, K.K., & Münck, E. (1989) J. Biol. Chem. 264, 21677-21681; Ming, L.-J., Que, L., Jr., Kriauciunas, A., Frolik, C.A., & Chen, V.J. (1990) Inorg. Chem. 26, 1111-1112]. These studies have revealed three coordinated His residues along with three sites for substrate [delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine, ACV], NO, and water binding on the active Fe(II) of IPNS. We report here NMR studies of Fe(II)IPNS and its Co(II)-substituted derivative [Co(II)IPNS]. By the use of NOE techniques on the Co(II)IPNS-ACV complex, we have recognized a -CH2-CH less than spin system at 14.6, 24.3, and 38.6 ppm that is assigned to the alpha and beta protons of a coordinated Asp residue. Corresponding solvent nonexchangeable features are found near 40 ppm in Fe(II)IPNS and the Fe(II)IPNS-ACV complex, but the peaks are too broad for NOE effects to be observed. The binding of NO to the Fe(II) center results in a significant change in the configuration of the metal site: (a) The C beta H2 resonances due to the coordinated Asp residue disappear. The loss of the signal may indicate a change of the carboxylate configuration from syn-like to anti-like or, less likely, its displacement by NO.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Chemistry, University of Minnesota, Minneapolis 55455.
authors.forename: L J
authors.initials: LJ
authors.lastname: Ming
authors.forename: L
authors.initials: L
authors.lastname: Que
authors.forename: A
authors.initials: A
authors.lastname: Kriauciunas
authors.forename: C A
authors.initials: CA
authors.lastname: Frolik
authors.forename: V J
authors.initials: VJ
authors.lastname: Chen
mesh_terms: D001665:Binding Sites; D004578:Electron Spin Resonance Spectroscopy; D006418:Heme / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D006418:Heme; D010088:Oxidoreductases; C038370:isopenicillin N synthetase