HPV 11, 16 and 18 DNA sequences in cervical swabs from women with cervical dysplasia: prevalence and associated risk of progression.
Cervical swabs obtained from 164 women with histologically proven preinvasive and invasive cervical neoplasia were analysed for HPV type 11, 16 and 18 DNA by filter in situ hybridisation. HPV 16 or 18 was detected in 8 of 24 swabs from patients with invasive squamous cell carcinoma (33%), in 59 of 100 patients with carcinoma in situ or severe dysplasia (59%) and in 16 of 40 patients with mild or moderate dysplasia (40%). HPV 6 or 11 was found in only 2% of all swabs. Thirty-eight of the patients participated in a prospective follow-up study and were monitored non-invasively by cytology and colposcopy for 8 to 36 months. 25 patients had persisting or progressive lesions, 13 of which harboured HPV 16 or 18. Of 13 patients who had complete resolution of the dysplasia, only 2 were HPV-positive. The study indicates a significantly higher risk of malignant progression when the cervical dysplasia is associated with HPV 16 or 18 infection.
Hørding
U
U
Department of Gyneacology and Obstetrics, Rigshospitalet, University Hospital, Copenhagen, Denmark.
Daugaard
S
S
Bock
J E
JE
Sebbelov
A M
AM
Norrild
B
B
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000382:microbiology; D002584:Cervix Uteri / Q000473:pathology; D003127:Colposcopy; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D011446:Prospective Studies; D014412:Tumor Virus Infections / Q000150:complications*; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000382:microbiology