[Effects of anti-asthmatic drugs on human eosinophil chemotaxis].
To evaluate the acute effects of anti-asthmatic drugs in vitro, we examined the modulation of various anti-asthmatic drugs in therapeutic concentrations on PAF-induced human eosinophil chemotaxis. Aminophylline (20 micrograms/ml) and Isoproterenol (10 nM) inhibited PAF (3 X 10(-8) M)-induced eosinophil chemotaxis nearly 30%, whereas no inhibitory effects were observed by Dexamethasone (0.1 microM), Tranilast, Ketotifen or Azelastine. Aminophylline (20 micrograms/ml) also inhibited LTB4 (3 X 10(-8) M)-induced eosinophil chemotaxis nearly 30%, whereas it did not inhibit chemotaxis induced by zymosan (5 mg/ml)-activated serum. These results indicate that anti-asthmatic drugs except for aminophylline and isoproterenol, when used acutely in therapeutic concentrations, have no striking inhibitory effects on PAF-induced eosinophil chemotaxis. These results further suggest the possibility that there are different mechanisms in eosinophil chemotaxis induced by PAF, LTB4 or by C5a.
Numao
T
T
Department of Medicine and Clinical Immunology, Dokkyo University School of Medicine, Tochigi, Japan.
Fukuda
T
T
Akutsu
I
I
Makino
S
S
D000628:Aminophylline / Q000494:pharmacology*; D001249:Asthma / Q000276:immunology*; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology; D004804:Eosinophils / Q000276:immunology*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D007975:Leukotriene B4 / Q000037:antagonists & inhibitors; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors
D010972:Platelet Activating Factor; D007975:Leukotriene B4; D000628:Aminophylline; D003907:Dexamethasone; D007545:Isoproterenol