title: Influence of wheat bran on NMU-induced mammary tumor development, plasma estrogen levels and estrogen excretion in female rats.
abstract: In our animal experiments the hypothesis was tested that a high-fiber (HF) diet reduces tumor promotion by interruption of the enterohepatic circulation resulting in lowered estrogen exposure of the estrogen-sensitive tissue. In the first experiment the development of N-nitrosomethylurea (NMU) induced mammary tumors was investigated. One group of rats (HF) was fed a HF diet (11% fiber, based on wheat bran), the other group (LF) fed a low-fiber diet (0.5% fiber, based on white wheat flour). Tumor incidence (90 and 80%, respectively) and latency (121 and 128 days, respectively) were similar in the HF and LF groups. Compared to the LF group, HF rats had lower tumor weights (0.16 vs 0.55 g; P less than 0.01) and a slightly lower tumor multiplicity (1.8 vs 2.8 tumors per tumor-bearing rat). These differences were reduced after adjustment for body weight. In a second experiment rats, not treated with the carcinogen, were kept on the same HF and LF diets. From these rats 24-h urine and feces and orbital blood samples were collected for analysis of (un)conjugated estrogens. The excretion of both free and conjugated estrogens in fecal samples was about 3-fold higher in HF rats than in LF rats. During the basal period of the cycle urinary excretion of estrone was lower in HF rats (mean 9.7 ng/day) than in LF rats (mean 13.0 ng/day; P less than 0.05). It is concluded that wheat bran interrupts the enterohepatic circulation of estrogens, but plasma levels are not affected. Whether the development of mammary tumors is reduced by the introduction of specific components of wheat bran, or by a reduced body weight due to a lower (effective) energy intake remains to be determined.
authors.affiliation: TNO Toxicology and Nutrition Institute, The Netherlands.
authors.forename: C J
authors.initials: CJ
authors.lastname: Arts
authors.forename: A T
authors.initials: AT
authors.lastname: de Bie
authors.forename: H
authors.initials: H
authors.lastname: van den Berg
authors.forename: P
authors.initials: P
authors.lastname: van 't Veer
authors.forename: G S
authors.initials: GS
authors.lastname: Bunnik
authors.forename: J H
authors.initials: JH
authors.lastname: Thijssen
mesh_terms: D000818:Animals; D004043:Dietary Fiber / Q000494:pharmacology*; D004967:Estrogens / Q000097:blood* / Q000652:urine; D005243:Feces / Q000201:enzymology; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D008770:Methylnitrosourea; D051381:Rats; D011916:Rats, Inbred F344; D014908:Triticum
chemical_list: D004043:Dietary Fiber; D004967:Estrogens; D008770:Methylnitrosourea; D005966:Glucuronidase