title: A comparison between immunofluorescence staining on smears from Membrana nictitans (M3 test), immunohistopathology and routine pathology in cats with suspected feline infectious peritonitis (FIP).
abstract: An indirect immunofluorescence method using smears from membrana nictitans (M3 test) to diagnose feline corona virus (FCV) infection was compared with immunohistopathology (using indirect immunofluorescence assay (IFFA) performed on organs (IFO], and routine pathology (RP) in cats with suspected feline infectious peritonitis (FIP). A close correlation between the 2 immunofluorescence methods (IFO and M3) was observed. Although the M3 test requires samples from only 1 organ per animal, both the sensitivity and specificity were high (80%), when compared to IFO (using samples from an average of 5 organs per animal). In 21% of the cats with suspected FIP typical pathological lesions were found. As the M3 test is relatively easy to perform, it could reduce work-load of pathology laboratories and provide valuable data for clinical and epidemiological use.
authors.affiliation: Department of Medical Microbial Ecology, Karolinska Institute, Stockholm, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Hök
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002415:Cats; D016765:Coronavirus, Feline / Q000276:immunology*; D016766:Feline Infectious Peritonitis / Q000175:diagnosis* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D009541:Nictitating Membrane / Q000382:microbiology*; D011237:Predictive Value of Tests
chemical_list: D000956:Antigens, Viral