title: Structural characterization of a novel glycosyl-phosphatidylinositol from the protozoan Tetrahymena mimbres.
abstract: A glycolipid metabolically labelled with [14C]GlcN was isolated from the free-living protozoan Tetrahymena mimbres. The glycolipid was sensitive to a bacterial phosphatidylinositol-specific phospholipase C, and the headgroup was shown to contain a phosphorylated Man alpha 1-2Man alpha 1-4Man alpha 1-4GlcN glycan. The Tetrahymena glycolipid is structurally unique among the glycosylphosphatidylinositols that have so far been characterized, including those from several protozoan parasites of humans.
authors.affiliation: Department of Biochemistry, University of Dundee, Scotland, U.K.
authors.forename: U
authors.initials: U
authors.lastname: Weinhart
authors.forename: J R
authors.initials: JR
authors.lastname: Thomas
authors.forename: Y B
authors.initials: YB
authors.lastname: Pak
authors.forename: G A
authors.initials: GA
authors.lastname: Thompson
authors.forename: M A
authors.initials: MA
authors.lastname: Ferguson
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D006017:Glycolipids / Q000737:chemistry* / Q000378:metabolism; D017261:Glycosylphosphatidylinositols; D008969:Molecular Sequence Data; D015394:Molecular Structure; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000737:chemistry* / Q000378:metabolism; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013768:Tetrahymena / Q000737:chemistry*
chemical_list: D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D010716:Phosphatidylinositols; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase