Purification and partial characterization of a soluble suppressor factor from papillomavirus-infected patients.
A molecular form of soluble suppressor factor was identified in serum-free supernatant fluid of cultured peripheral blood mononuclear cells (PBMC) from patients with severe forms of papillomavirus infections (epidermodysplasia verruciformis and large treatment-resistant condyloma acuminatum). The papillomavirus-induced soluble suppressor factor (SSF-H) was highly purified by gel filtration, anion-exchange chromatography, chromatofocusing, hydrophobic chromatography, and nondissociating polyacrylamide gel electrophoresis (PAGE). The factor exhibited an apparent molecular weight of 67 kDa as determined by sodium dodecylsulfate-PAGE. The isoelectric point ranged from 4.8 to 5.2 as judged by chromatofocusing. The biologic activity of the SSF-H during purification and in subsequent characterization was examined by in vitro CTLL bioassay. Purified SSF-H was stable at 56 degrees C for 1 h and in the pH range of 4.0 to 8.0. Complete inactivation was observed at 80 degrees C. Trypsin and proteinase K treatment destroyed the biologic activity associated with purified SSF-H. This factor seems to be different in its molecular weight and other physicochemical properties from the known suppressor factors that affect lymphocyte functions.
Chopra
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Department of Microbiology, University of Texas Medical Branch, Galveston.
Chopra
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Tyring
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D001685:Biological Factors / Q000302:isolation & purification* / Q000378:metabolism; D002478:Cells, Cultured; D002845:Chromatography; D003218:Condylomata Acuminata / Q000097:blood; D003470:Culture Media / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004819:Epidermodysplasia Verruciformis / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008970:Molecular Weight; D027383:Papillomaviridae; D011489:Protein Denaturation; D014412:Tumor Virus Infections / Q000097:blood*
D001685:Biological Factors; D003470:Culture Media