High-performance liquid chromatographic determination of 18 alpha-glycyrrhetinic acid and 18 beta-glycyrrhetinic acid in rat plasma: application to pharmacokinetic study.
A high-performance liquid chromatographic method for the separation and determination of 18 alpha-glycyrrhetinic acid and 18 beta-glycyrrhetinic acid has been developed. Indomethacin was used as an internal standard. The drugs were separated on a reversed-phase column and detected by UV detection at a wavelength of 254 nm. Methanol-water-perchloric acid-ammonia (80:20:0.4:0.4, v/v) was used as the mobile phase at pH of 7.0-7.5. The detection limit of both compounds was 0.1 microgram/ml in rat plasma. The method was applied to pharmacokinetic studies of glycyrrhetinic acids in rats. The results suggest that the pharmacokinetics appeared to be non-linear in nature.
Tsai
T H
TH
National Research Institute of Chinese Medicine, Taipei, Taiwan.
Chen
C F
CF
D000641:Ammonia; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006034:Glycyrrhetinic Acid / Q000097:blood* / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D008297:Male; D010472:Perchlorates; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D010472:Perchlorates; D000641:Ammonia; D006034:Glycyrrhetinic Acid