Suppression of tumorigenicity in Wilms tumor by the p15.5-p14 region of chromosome 11.
Wilms tumor has been associated with genomic alterations at both the 11p13 and 11p15 regions. To differentiate between the involvement of these two loci, a chromosome 11 was constructed that had one or the other region deleted, and this chromosome was introduced into the tumorigenic Wilms tumor cell line G401. When assayed for tumor-forming activity in nude mice, the 11p13-deleted, but not the 11p15.5-p14.1-deleted chromosome, retained its ability to suppress tumor formation. These results provide in vivo functional evidence for the existence of a second genetic locus (WT2) involved in suppressing the tumorigenic phenotype of Wilms tumor.
Dowdy
S F
SF
Department of Microbiology and Molecular Genetics, University of California, Irvine 92717.
Fasching
C L
CL
Araujo
D
D
Lai
K M
KM
Livanos
E
E
Weissman
B E
BE
Stanbridge
E J
EJ
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D016162:Genes, Wilms Tumor / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D007680:Kidney Neoplasms / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D009396:Wilms Tumor / Q000235:genetics*