Monoclonal antibodies to gp100 inhibit penetration of human herpesvirus 6 and polykaryocyte formation in susceptible cells.
We report the derivation and properties of a monoclonal antibody (MAb 2E4) which neutralizes human herpesvirus 6 (HHV-6). MAb 2E4 precipitated from lysates of infected cells a glycosylated polypeptide 100,000 in apparent molecular weight and minor components of 80,000, and 32,500. The predominant reactive protein after a pulse was the 100,000-molecular-weight peptide designated as gp100. The smaller polypeptides appeared in the precipitate predominantly after a chase. MAb 2E4 neutralized HHV-6 infectivity in the presence and in the absence of complement, and it inhibited the penetration of virus into the cells. Addition of MAb 2E4 as late as 6 h postinfection inhibited the formation of large polykaryocytes typical of HHV-6-infected cells.
Foà-Tomasi
L
L
Department of Experimental Pathology, University of Bologna, Italy.
Boscaro
A
A
di Gaeta
S
S
Campadelli-Fiume
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D002459:Cell Fusion; D003165:Complement System Proteins / Q000276:immunology; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D015726:Giant Cells / Q000276:immunology*; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D015654:Herpesvirus 6, Human / Q000276:immunology* / Q000502:physiology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011233:Precipitin Tests; D013601:T-Lymphocytes / Q000166:cytology / Q000382:microbiology*; D014764:Viral Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D014764:Viral Proteins; D003165:Complement System Proteins