Affinity-purified soluble Fc epsilon RII/CD23 derived from a culture supernatant of an EBV-immortalized B-cell line induced a monophasic fever in rabbits.
A soluble form of Fc epsilon RII/CD23 is spontaneously released from most lymphoblastoid cell lines established by the Epstein-Barr virus (EBV). Such a product was purified on an IgE-Sepharose column and its pyrogenic effect was investigated in rabbits. This preparation induced a monophasic fever in rabbits, with a peak response appearing 75 min after injection. Since IgE was found to be capable of abrogating such an effect, it is suggested that IgE might be involved in the control of the effectiveness of this soluble protein.
Ghaderi
A A
AA
Department of Microbiology and Immunology, Medical School, Shiraz University, Iran.
Stanworth
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DR
D000818:Animals; D015703:Antigens, CD / Q000502:physiology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology*; D002472:Cell Transformation, Viral / Q000276:immunology*; D002478:Cells, Cultured; D005334:Fever / Q000276:immunology*; D004854:Herpesvirus 4, Human; D007073:Immunoglobulin E / Q000502:physiology; D008297:Male; D011817:Rabbits; D011961:Receptors, Fc / Q000502:physiology*; D017455:Receptors, IgE
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E