title: S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function.
abstract: We have identified mutant strains of S. cerevisiae that fail to properly arrest their cell cycles at mitosis in response to the loss of microtubule function. New bud emergence and DNA replication (but not cytokinesis) occur with high efficiency in the mutants under conditions that inhibit these events in wild-type cells. The inability to halt cell cycle progression is specific for impaired microtubule function; the mutants respond normally to other cell cycle-blocking treatments. Under microtubule-disrupting conditions, the mutants neither achieve nor maintain the high level of histone H1 kinase activity characteristic of wild-type cells. Our studies have defined three genes required for normal cell cycle arrest. These findings are consistent with the existence of a surveillance system that halts the cell cycle in response to microtubule perturbation.
authors.affiliation: Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
authors.forename: M A
authors.initials: MA
authors.lastname: Hoyt
authors.forename: L
authors.initials: L
authors.lastname: Totis
authors.forename: B T
authors.initials: BT
authors.lastname: Roberts
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001542:Benomyl / Q000494:pharmacology; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D002455:Cell Division; D003001:Cloning, Molecular; D004261:DNA Replication; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008870:Microtubules / Q000502:physiology*; D008969:Molecular Sequence Data; D015739:Nocodazole / Q000494:pharmacology; D006656:Protamine Kinase / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: C070351:BUB2 protein, S cerevisiae; C070352:BUB3 protein, S cerevisiae; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D006656:Protamine Kinase; D015739:Nocodazole; D001542:Benomyl