title: T cell receptor gamma and delta gene junctional sequences in SCID mice: excessive P nucleotide insertion.
abstract: The severe combined immunodeficiency (SCID) mutation has been postulated to affect a V(D)J recombinase activity involved in coding joint formation. Analysis of 38 joints from 34 distinct sequences of normally rearranged T cell receptor (TCR) gamma and delta genes from adult, SCID thymocytes reveals coding joints with an increased number of P nucleotides. One-third of P sequences are greater than or equal to 4 nucleotides in length and P elements of up to 15 bases are observed. This suggests that the SCID defect deregulates P nucleotide addition. Consequently, essential V(D)J recombination intermediates may seldom be generated.
authors.affiliation: Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: L J
authors.initials: LJ
authors.lastname: Kienker
authors.forename: W A
authors.initials: WA
authors.lastname: Kuziel
authors.forename: P W
authors.initials: PW
authors.lastname: Tucker
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004251:DNA Transposable Elements; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D016511:Severe Combined Immunodeficiency / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D045567:VDJ Recombinases
chemical_list: D004251:DNA Transposable Elements; D009838:Oligodeoxyribonucleotides; D016692:Receptors, Antigen, T-Cell, gamma-delta; D004247:DNA; D004254:DNA Nucleotidyltransferases; D045567:VDJ Recombinases