title: Aerobic 2-ketogluconate metabolism of Klebsiella pneumoniae NCTC 418 grown in chemostat culture.
abstract: Klebsiella pneumoniae NCTC 418 is able to convert 2-ketogluconate intracellularly to 6-phosphogluconate by the combined action of an NADPH-dependent 2-ketogluconate reductase and gluconate kinase. Synthesis of the former enzyme was maximal under 2-ketogluconate-limited growth conditions. An instantaneous transition to a 2-ketogluconate-excess condition resulted in an acceleration of catabolism of this carbon source, accompanied by complete inhibition of biosynthesis. It is suggested that the cause of this inhibition resides in depletion of the NADPH pool due to the high rate at which NADPH is oxidized by 2-ketogluconate reductase.
authors.affiliation: Department of Microbiology, Biotechnology Centre, University of Amsterdam, The Netherlands.
authors.forename: J A
authors.initials: JA
authors.lastname: Simons
authors.forename: M J
authors.initials: MJ
authors.lastname: Teixeira de Mattos
authors.forename: O M
authors.initials: OM
authors.lastname: Neijssel
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000332:Aerobiosis; D002237:Carbohydrate Dehydrogenases / Q000096:biosynthesis / Q000378:metabolism*; D002458:Cell Fractionation; D005942:Gluconates / Q000378:metabolism*; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor)
chemical_list: D005942:Gluconates; C008229:2-ketogluconate; D009249:NADP; D000255:Adenosine Triphosphate; D002237:Carbohydrate Dehydrogenases; C020577:gluconate 2-dehydrogenase; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C020578:gluconokinase