Further evidence for a gamma/delta T cell receptor-mediated TCT.1/CD48 recognition.
We have demonstrated recently that a molecule, termed TCT.1 (Blast-1/CD48), is recognized on the surface of target cells by a series of alloreactive gamma/delta T cell clones generated from PBL of one healthy individual (designated E). Southern blot analyses suggested that these clones express a TCR associating a V3-JP2-C2 gamma-chain and V1-D-J1-C delta-chain. In the present study, we have developed from PBL of a second normal donor (designated G) a novel series of gamma/delta cloned T cell lines with similar functional activity (i.e., specific recognition of TCT.1 protein). The TCR gamma- and delta-chain nucleotide sequences of both the E and G clones were determined. Results show that 1) sequences from all the clones are identical in each individual donor, 2) the delta-chains expressed by the E and the G clones are encoded by distinct gene rearrangements including V1-D-J delta 1 and V1-D-J delta 2, respectively, 3) the gamma-chains expressed by the E and the G clones are encoded by the same genomic variable elements, namely V gamma 3 and JP2, whereas the junctional regions are distinct. Because the latter rearrangement is very infrequent in human peripheral blood, these data support the view that TCT.1/CD48 recognition is likely to be TCR dependent.
Mami-Chouaib
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F
Laboratoire d'Hémato-Immunologie, INSERM, U333, Institut Gustave Roussy, Villejuif, France.
Del Porto
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P
Delorme
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D
Hercend
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T
D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000276:immunology*; D001483:Base Sequence; D000071178:CD48 Antigen; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology*; D013329:Structure-Activity Relationship; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015703:Antigens, CD; D000071178:CD48 Antigen; C000604065:CD48 protein, human; D016692:Receptors, Antigen, T-Cell, gamma-delta