Alphoid DNA polymorphisms for chromosome 21 can be distinguished from those of chromosome 13 using probes homologous to both.
Although alphoid DNA sequences shared among acrocentric chromosomes have been identified, no human chromosome 21-specific sequence has been isolated from the centromeric region. To identify alphoid DNA restriction fragment length polymorphisms (RFLPs) specific for chromosome 21, we hybridized human genomic DNA with alphoid DNA probes [L1.26; aRI(680),21-208] shared by chromosomes 13 and 21. We detected RFLPs with restriction enzymes ECoRI, HaeIII, MboI,StuI, and TaqI. The segregation of these RFLPs was analyzed in the 40 CEPH families. Linkage analysis between these RFLPs and loci previously mapped to either chromosome 13 or 21 revealed RFLPs that appear to be specific to chromosome 21. These polymorphisms may be useful as genetic markers of the centromeric region of chromosome 21. Different alphoid loci within the centromeric region of chromosome 13 were identified.
Jabs
E W
EW
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Warren
A C
AC
Taylor
E W
EW
Colyer
C R
CR
Meyers
D A
DA
Antonarakis
S E
SE
D002503:Centromere / Q000648:ultrastructure*; D002882:Chromosomes, Human, Pair 13; D002891:Chromosomes, Human, Pair 21; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D008040:Genetic Linkage; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004247:DNA