Fast PCR based therapeutic follow-up of T-cell malignancies.
Available methods for the detection of minimal residual disease in T-cell malignancies are limited by their poor sensitivity and/or by their complexity. With the aim of avoiding these drawbacks, we used the Fast PCR technique in order to amplify V delta 1-(D delta 1)-(D delta 2)-J delta 1 and V gamma I family-J gamma junctional sequences from nucleated cells of boiled bone marrow. We were thus able to detect malignant T-cells down to a dilution of 1 in 665 nucleated marrow cells, in less than 4 hours after sampling. This new quantitative method is promising for monitoring therapy and detecting early disease relapse in T-lymphoproliferative disease, since it is 2 to 35 fold more sensitive than Southern blotting.
Vandenvelde
C
C
Virology Department, Brugmann University Hospital, Brussels, Belgium.
Scheen
R
R
Duys
M
M
Corazza
F
F
Van Beers
D
D
D001483:Base Sequence; D001856:Bone Marrow Examination / Q000379:methods*; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000235:genetics; D005500:Follow-Up Studies; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D007938:Leukemia / Q000235:genetics; D015458:Leukemia, T-Cell / Q000235:genetics* / Q000473:pathology; D008223:Lymphoma; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000235:genetics; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D004273:DNA, Neoplasm