title: Signal transduction pathways in the induction of HLA class I antigen expression on Huh 6 cells by interferon-gamma.
abstract: This study investigated the intracellular signal transduction regulating the appearance of HLA class I antigens on Huh 6 cells induced by interferon-gamma. The expression was blocked by a protein kinase C inhibitor, H-7, but not by a calmodulin antagonist, W-7, nor by a protein kinase A inhibitor, H-8, at low dose. The antigen expression was induced by a direct activator of protein kinase C, phorbol myristate acetate, but not by calcium ionophore A23187 nor an analog of cAMP, dbcAMP. Therefore, we concluded that protein kinase C is involved in the expression of HLA class I antigens on Huh 6 cells induced by interferon-gamma but Ca(2+)-calmodulin and cAMP are not.
authors.affiliation: First Department of Medicine, Osaka University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Towata
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Katayama
authors.forename: T
authors.initials: T
authors.lastname: Takehara
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaki
authors.forename: A
authors.initials: A
authors.lastname: Kasahara
authors.forename: H
authors.initials: H
authors.lastname: Fusamoto
authors.forename: T
authors.initials: T
authors.lastname: Kamada
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000001:Calcimycin / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D002460:Cell Line; D003563:Cyclic CMP / Q000031:analogs & derivatives / Q000494:pharmacology; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007546:Isoquinolines / Q000494:pharmacology; D008113:Liver Neoplasms / Q000276:immunology*; D010879:Piperazines / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*; D013449:Sulfonamides / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D002147:Calmodulin; D015395:Histocompatibility Antigens Class I; D007546:Isoquinolines; D010879:Piperazines; D013449:Sulfonamides; D003563:Cyclic CMP; D000001:Calcimycin; C053441:dibutyryl cyclic-3',5'-cytidine monophosphate; C017967:W 7; D007371:Interferon-gamma; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; D013755:Tetradecanoylphorbol Acetate