title: IS10 promotes adjacent deletions at low frequency.
abstract: Some transposable elements move by a replicative mechanism involving cointegrate formation. Intramolecular cointegration can generate a product called an "adjacent deletion" in which a contiguous chromosomal segment adjacent to the transposon is deleted while the element responsible remains intact. Insertion sequence IS10 is thought to transpose by a nonreplicative mechanism. In the simplest models, nonreplicative transposition cannot give rise to an adjacent deletion because an intrinsic feature of such transposition is excision of the IS element from the donor location. We report here that IS10 can generate adjacent deletions, but at a frequency which is approximately 1/30th the frequency of transposition for the same element. We suggest that these deletions might arise either by nonreplicative transposition events that involve two IS10 elements located on sister chromosomes or by aberrant nonreplicative events involving cleavage and ligation at only one end of the element.
authors.affiliation: Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
authors.forename: D E
authors.initials: DE
authors.lastname: Roberts
authors.forename: D
authors.initials: D
authors.lastname: Ascherman
authors.forename: N
authors.initials: N
authors.lastname: Kleckner
mesh_terms: D015139:Blotting, Southern; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009876:Operon; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements