Antibody assays suitable for assessing immune responses to live varicella vaccine.
An enzyme-linked immunosorbent assay for antibodies to varicella-zoster virus (VZV), using purified viral glycoproteins as antigen (gpELISA), was compared with other assays for measuring vaccine-induced antibody responses. The gpELISA was more sensitive than conventional assays, proved highly specific for VZV and agreed well with an assay for neutralizing antibody activity. It was successfully applied to large-scale testing of live varicella vaccine in humans.
Provost
P J
PJ
Cellular and Molecular Biology, Merck Sharp & Dohme Research Laboratories, West Point, PA 19486.
Krah
D L
DL
Kuter
B J
BJ
Morton
D H
DH
Schofield
T L
TL
Wasmuth
E H
EH
White
C J
CJ
Miller
W J
WJ
Ellis
R W
RW
D000914:Antibodies, Viral / Q000032:analysis* / Q000096:biosynthesis; D000956:Antigens, Viral; D019433:Chickenpox Vaccine; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D009500:Neutralization Tests; D014764:Viral Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D019433:Chickenpox Vaccine; D006023:Glycoproteins; D014764:Viral Proteins; D014765:Viral Vaccines