title: Adrenergic and cholinergic regulation of phospholipid release in sublingual salivary gland in vitro.
abstract: 1. The role of adrenergic and cholinergic mediators in the regulation of salivary phospholipid secretion was investigated using rat sublingual acinar cells maintained in the presence of [3H]choline. 2. The release of [3H]choline containing phospholipids was enhanced by beta-adrenergic agonist, isoproterenol to a greater extent than the cholinergic agonist, pilocarpine. 3. The effect of isoproterenol on phospholipid release was observed even after 5 min and by 30 min a 1.7-fold increase in secretion occurred, whereas pilocarpine evoked 1.2-fold increase by 30 min with no discernible effect in 5 min. The isoproterenol effect was blocked by alprenolol, and an inhibition of pilocarpine effect was observed with atropine. 4. In the absence of mediators, 82% of secreted labeled phospholipids were represented by phosphatidylcholine (PC), 4.3% by lysophosphatidylcholine (LPC) and 12.1% by sphingomyeline (Sph), those secreted in the presence of isoproterenol showed significantly lower (37%) content of LPC, while those secreted in response to pilocarpine were substantially richer in LPC (2.5 times) and Sph (27%), and contained less (17%) PC. 5. The results provide first evidence for the involvement of adrenergic and cholinergic mediators in the regulation of salivary phospholipid secretion.
authors.affiliation: Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
authors.forename: B L
authors.initials: BL
authors.lastname: Slomiany
authors.forename: S
authors.initials: S
authors.lastname: Sengupta
authors.forename: Z
authors.initials: Z
authors.lastname: Fekete
authors.forename: V L
authors.initials: VL
authors.lastname: Murty
authors.forename: A
authors.initials: A
authors.lastname: Slomiany
mesh_terms: D000526:Alprenolol / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002794:Choline / Q000378:metabolism; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D010275:Parasympathetic Nervous System / Q000502:physiology*; D010743:Phospholipids / Q000378:metabolism*; D010862:Pilocarpine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D012469:Salivary Glands / Q000378:metabolism*; D013564:Sympathetic Nervous System / Q000502:physiology*
chemical_list: D010743:Phospholipids; D011943:Receptors, Adrenergic, beta; D010862:Pilocarpine; D001285:Atropine; D000526:Alprenolol; D007545:Isoproterenol; D002794:Choline