Phosphatidylserine increases the affinity of the AMPA/quisqualate receptor in rat brain membranes.
We investigated the effects of phospholipids and cholesterol on the binding of [3H]-AMPA to rat telencephalic membranes. Phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and cholesterol were without effect at concentrations up to 1.5 mg/mg protein. Only phosphatidylserine increased [3H]-AMPA binding in a dose-dependent manner. This effect was due to an increase in the affinity of the low affinity component of [3H]-AMPA binding. These results indicate that the distribution of phosphatidylserine in membranes modulates the properties of the AMPA/quisqualate receptor.
Baudry
M
M
Program in Neural, Informational, and Behavioral Sciences, University of Southern California, Los Angeles 90089-2520.
Massicotte
G
G
Hauge
S
S
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000502:physiology; D002784:Cholesterol / Q000494:pharmacology; D007051:Ibotenic Acid / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D010713:Phosphatidylcholines / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010718:Phosphatidylserines / Q000494:pharmacology*; D051381:Rats; D018091:Receptors, AMPA; D017981:Receptors, Neurotransmitter / Q000187:drug effects* / Q000502:physiology; D013109:Sphingomyelins / Q000494:pharmacology; D013570:Synaptic Membranes / Q000187:drug effects* / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects* / Q000502:physiology; D013687:Telencephalon / Q000187:drug effects / Q000502:physiology; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D018091:Receptors, AMPA; D017981:Receptors, Neurotransmitter; D013109:Sphingomyelins; D007051:Ibotenic Acid; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D002784:Cholesterol