Parathyroid hormone enhances gentamicin uptake by opossum kidney cells but not by LLC-PK1 cells.
Parathyroidectomy is proposed to be protective against gentamicin nephrotoxicity in rats. In the present study, we evaluated the effects of bovine PTH(1-34) on gentamicin accumulation in cultured kidney epithelial cell lines, opossum kidney (OK) cells which possess PTH receptors and LLC-PK1 cells which are devoid of PTH receptors. Ten days after seeding, the culture medium was exchanged for medium containing 1 mM gentamicin and bovine PTH. The cell gentamicin concentration was measured by a substrate-labeled fluorescence immunoassay (TDA gentamicin kit). Gentamicin uptake was accelerated by bovine PTH in OK cells but not in LLC-PK1 cells. The enhancing effect of bovine PTH seems to be mediated by a cAMP-dependent process. The results suggest that PTH accelerates gentamicin accumulation in renal tissues and potentiates gentamicin nephrotoxicity.
Nakahama
H
H
First Department of Medicine, Osaka University Medical School, Japan.
Kakihara
M
M
Fukuhara
Y
Y
Ueda
N
N
Orita
Y
Y
Kamada
T
T
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D005839:Gentamicins / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009893:Opossums; D010281:Parathyroid Hormone / Q000502:physiology*; D011506:Proteins / Q000378:metabolism; D013552:Swine
D005839:Gentamicins; D010281:Parathyroid Hormone; D011506:Proteins; D003994:Bucladesine; D000242:Cyclic AMP