Conjugal transfer of recombinant transposon Tn916 from Escherichia coli to Bacillus stearothermophilus.
A gene for thermostable amylase has been inserted at the BstXI site of Tn916. Mating experiments demonstrated that unlike Tn916, the recombinant transposon, designated Tn916A, could transfer from Escherichia coli to Bacillus stearothermophilus BR219 in broth matings, resulting in chromosomal integration of the transposon and expression of the amylase at significant levels.
Natarajan
M R
MR
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824.
Oriel
P
P
D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype; D001411:Geobacillus stearothermophilus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic; D015183:Restriction Mapping; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D000516:alpha-Amylases