Hemagglutination and hemagglutination inhibition with Chuzan virus.
Chuzan virus agglutinated erythrocytes of several species of animals including bovine. The hemagglutinating (HA) activity against bovine erythrocytes was dependent on NaCl molarity and was expressed best at 0.6 M, but it was independent of pH and temperature. Three strains of Chuzan virus isolated from 2 cows and a pool of culicoides midges had indistinguishable HA antigenicity. All cattle infected with the virus developed high titers of hemagglutination inhibiting (HI) antibody which changed in parallel with neutralizing (NT) antibody titers. Correlation between HI and NT antibodies was very high and the antibodies persisted for one year or more. Therefore it was concluded that the HI test is applicable for survey of Chuzan virus infection among cattle in place of the NT test.
Goto
Y
Y
Kyushu Branch Laboratory, National Institute of Animal Health, Kagoshima, Japan.
Miura
Y
Y
Kono
Y
Y
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology; D002460:Cell Line; D002519:Ceratopogonidae / Q000382:microbiology; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006387:Hemagglutination, Viral; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera / Q000276:immunology; D009500:Neutralization Tests; D009994:Osmolar Concentration; D012087:Reoviridae / Q000276:immunology* / Q000302:isolation & purification; D012088:Reoviridae Infections / Q000276:immunology / Q000382:microbiology; D013696:Temperature
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera