Detection of mannophosphoinositide antigens in sputum of tuberculosis patients by dot enzyme immunoassay.
A simple and economical dot ELISA for the detection of mannophosphoinositide antigen in sputum samples of tuberculosis patients has been developed using affinity-purified antibodies. This test is able to detect free as well as bound antigen. Sputum samples from 94 patients suffering from tuberculosis and 30 non-tuberculosis patients were screened and an overall sensitivity and specificity of 89% and 93.3%, respectively, was obtained. The sensitivity of the test among the different groups of tuberculosis patients did not vary significantly.
Kansal
R
R
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Khuller
G K
GK
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010716:Phosphatidylinositols / Q000276:immunology*; D011817:Rabbits; D013183:Sputum / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
D000907:Antibodies, Bacterial; D010716:Phosphatidylinositols; C002423:mannophosphoinositide