title: Construction of cloning vectors for Bacillus thuringiensis.
abstract: The replication region of the Bacillus thuringiensis plasmid, pHT1030, was treated with hydroxylamine. Various copy-number mutants were selected and subsequently used to construct shuttle vectors with multiple cloning sites. These recombinant plasmids are very stable and allowed the cloning of a delta-endotoxin-encoding gene in B. thuringiensis. Comparison between gene expression level and vector copy-number indicated that a plateau in delta-endotoxin production is reached with a copy-number of about fifteen per equivalent chromosome.
authors.affiliation: Unité de Biochimie Microbienne, URA 1300, Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.
authors.forename: O
authors.initials: O
authors.lastname: Arantes
authors.forename: D
authors.initials: D
authors.lastname: Lereclus
mesh_terms: D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003001:Cloning, Molecular; D004261:DNA Replication / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006460:Hemolysin Proteins; D019811:Hydroxylamine; D006898:Hydroxylamines; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D006898:Hydroxylamines; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D019811:Hydroxylamine