Characteristics of estrogen-induced peroxidase in mouse uterine luminal fluid.
Peroxidase activity in the uterine luminal fluid of mice treated with diethylstilbestrol was measured by the guaiacol assay and also by the formation of 3H2O from [2-3H]estradiol. In the radiometric assay, the generation of 3H2O and 3H-labeled water-soluble products was dependent on H2O2 (25 to 100 microM), with higher concentrations being inhibitory. Tyrosine or 2,4-dichlorophenol strongly enhanced the reaction catalyzed either by the luminal fluid peroxidase or the enzyme in the CaCl2 extract of the uterus, but decreased the formation of 3H2O from [2-3H]estradiol by lactoperoxidase in the presence of H2O2 (80 microM). NADPH, ascorbate, and cytochrome c inhibited both luminal fluid and uterine tissue peroxidase activity to the same extent, while superoxide dismutase showed a marginal activating effect. Lactoferrin, a major protein component of uterine luminal fluid, was shown not to contribute to its peroxidative activity, and such an effect by prostaglandin synthase was also ruled out. However, it was not possible to exclude eosinophil peroxidase, brought to the uterus after estrogen stimulation, as being the source of peroxidase activity in uterine luminal fluid.
Jellinck
P H
PH
Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.
Newbold
R R
RR
McLachlan
J A
JA
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001826:Body Fluids / Q000201:enzymology*; D002733:Chlorophenols / Q000494:pharmacology; D003574:Cytochrome c Group / Q000494:pharmacology; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007784:Lactoperoxidase / Q000378:metabolism; D051379:Mice; D009249:NADP / Q000494:pharmacology; D009195:Peroxidase / Q000096:biosynthesis*; D013482:Superoxide Dismutase / Q000494:pharmacology; D014316:Tritium; D014443:Tyrosine / Q000494:pharmacology; D014599:Uterus / Q000187:drug effects / Q000201:enzymology*; D014867:Water / Q000378:metabolism
D002733:Chlorophenols; D003574:Cytochrome c Group; D014867:Water; D014316:Tritium; D014443:Tyrosine; D004958:Estradiol; D009249:NADP; D004054:Diethylstilbestrol; D006861:Hydrogen Peroxide; D007784:Lactoperoxidase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid; C004762:2,4-dichlorophenol