Defective expression of neutrophil C3b receptors and impaired lymphocyte Na(+)-K(+)-ATPase activity in patients with systemic lupus erythematosus.
In our previous report, we demonstrated that the functions of phagocytes and lymphocytes were defective in patients with systemic lupus erythematosus (SLE). In an attempt to further clarify the defective mechanisms of these cells, 25 active SLE, 10 bronchial asthma patients (BA) on corticosteroids and 25 age and sex-matched normal individuals were investigated for the expression of membraneous C3b receptors, ionophore-induced 45Ca(2+)-uptake, mitochondrial potentials and phagocytic activity of neutrophils. We found decreased expression of C3b receptors on SLE PMN in both resting (37.2 +/- 3.7% of the normal controls) and FMLP-stimulated (68.3 +/- 7.1% of the normal controls) conditions, whereas the C3b receptor expression on BA-PMN receiving long-term steroid treatment was not different from normal controls. This suggests that the defective phagocytosis of SLE PMN is in the recognition, but not in the ingestion phase because of the normal function of Ca(2+)-influx and mitochondrial activity in SLE PMN. On the other hand, hyporesponsiveness to PHA stimulation (stimulation index: 127.4 +/- 46.3 in SLE vs. 311.2 +/- 30.4 in normals, p = 0.0077) was a distinct cell-mediated immune abnormality in our SLE patients. We measured the membrane potential of individual cells using 3,3'-dihexyloxacarbocyanin and found hyperpolarization in resting SLE lymphocytes. However, the membrane polarization of SLE lymphocytes became lower than that of normal cells after PHA stimulation for 3 days. A similar tendency was also found in Na(+)-K(+)-dependent ATPase activity in SLE lymphocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
Yu
C L
CL
Department of Medicine, Veterans General Hospital-Taipei, Taiwan, R.O.C.
Tsai
C Y
CY
Chiu
C C
CC
Liao
T S
TS
Lin
W M
WM
Chiang
B N
BN
Han
S H
SH
Wang
S R
SR
D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D004198:Disease Susceptibility / Q000209:etiology; D005260:Female; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000209:etiology*; D007239:Infections / Q000209:etiology; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000201:enzymology*; D008564:Membrane Potentials; D008875:Middle Aged; D009504:Neutrophils / Q000737:chemistry* / Q000187:drug effects; D010587:Phagocytosis; D011951:Receptors, Complement / Q000096:biosynthesis*; D017463:Receptors, Complement 3b; D000254:Sodium-Potassium-Exchanging ATPase / Q000172:deficiency*
D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D000001:Calcimycin; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium