title: Characterization of two insertion sequences, IS701 and IS702, from the cyanobacterium Calothrix species PCC 7601.
abstract: We describe the characterization of two insertion elements, IS701 and IS702, isolated from Calothrix species PCC 7601. These insertion elements were cloned from spontaneous pigmentation mutants. Both show the characteristics of typical bacterial insertion sequences, i.e. they present long terminal inverted repeats and they duplicate target DNA upon insertion. These elements share no homology with the only other cyanobacterial insertion sequence described so far, IS891. At least 15 copies of IS701 and 9 copies of IS702 were detected by hybridization experiments in the Calothrix 7601 genome. Their occurrence in several cyanobacterial strains is also reported.
authors.affiliation: Unité de Physiologie Microbienne (CNRS, URA1129), Institut Pasteur, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Mazel
authors.forename: C
authors.initials: C
authors.lastname: Bernard
authors.forename: R
authors.initials: R
authors.lastname: Schwarz
authors.forename: A M
authors.initials: AM
authors.lastname: Castets
authors.forename: J
authors.initials: J
authors.lastname: Houmard
authors.forename: N
authors.initials: N
authors.lastname: Tandeau de Marsac
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D015723:Gene Library; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D010798:Phycocyanin / Q000235:genetics; D010799:Phycoerythrin / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements; D010798:Phycocyanin; D010799:Phycoerythrin