title: Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose.
abstract: Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogenous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.
authors.affiliation: Laboratorio de Virología, Edificio A Segundo Piso, Facultad de Medicina, Universidad Nacional Autónoma de México, México City.
authors.forename: J
authors.initials: J
authors.lastname: Bustos
authors.forename: P
authors.initials: P
authors.lastname: Zamora
authors.forename: E
authors.initials: E
authors.lastname: Mejía
authors.forename: Y
authors.initials: Y
authors.lastname: Varela
authors.forename: B
authors.initials: B
authors.lastname: Gómez
mesh_terms: D002500:Centrifugation, Isopycnic / Q000379:methods*; D005069:Evaluation Studies as Topic; D011205:Povidone; D012412:Rubella virus / Q000302:isolation & purification*; D012822:Silicon Dioxide; D013395:Sucrose
chemical_list: D013395:Sucrose; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone