Cellular retinoic acid-binding protein in rat lacrimal gland.
We employed a monoclonal antibody to cellular retinoic acid-binding protein (CRABP) to assess the presence and localization of this retinoid-binding protein in the lacrimal gland of the rat. Immunoblots of extracts of rat lacrimal gland showed specific immunostaining of lacrimal CRABP in the region 14-16 kDa. Sections of rat lacrimal glands that were stained with anti-CRABP antibodies showed reaction product in the cytoplasm of the acinar cells. Retinoic acid may play a role in maintaining the proper function of lacrimal gland cells.
Yamaguchi
K
K
Department of Ophthalmology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157.
Gaur
V P
VP
Young
R W
RW
Sweatt
A J
AJ
D000818:Animals; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins / Q000378:metabolism*; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007765:Lacrimal Apparatus / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D018168:Receptors, Retinoic Acid; D013737:Testis / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D018168:Receptors, Retinoic Acid; D014212:Tretinoin