Nuclear targeting of a viral-cointernalized protein by a short signal sequence from human retinoic acid receptors.
The synthesis of a putative nuclear localization signal from the human retinoic acid receptor is described. Its ability to target a carrier protein to the nucleus is demonstrated following microinjection or a new technique of cell internalization in the presence of adenovirus 3.
Hamy
F
F
INSERM U16, Laboratoire de Biochimie Structurale, Faculté de Médecine, Lille, France.
Verwaerde
P
P
Helbecque
N
N
Formstecher
P
P
Hénichart
J P
JP
D000256:Adenoviridae / Q000378:metabolism*; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry*; D002467:Cell Nucleus / Q000378:metabolism* / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000737:chemistry / Q000378:metabolism*; D018168:Receptors, Retinoic Acid; D012710:Serum Albumin, Bovine / Q000378:metabolism
D002352:Carrier Proteins; D021382:Protein Sorting Signals; D018168:Receptors, Retinoic Acid; D012710:Serum Albumin, Bovine