title: Complementary roles for CD2 and LFA-1 adhesion pathways during T cell activation.
abstract: The influence of T cell receptor (TcR) triggering on T cell adhesion function has been systematically investigated in the present studies; we show that the adhesion function of LFA-1 is minimal in non-activated T cells but is augmented within minutes following TcR-mediated activation. In contrast, CD2 function is essentially optimal in non-activated T cells and undergoes no detectable modification within 12 h of TcR stimulation. Protein kinase C activation augments LFA-1 but not CD2 adhesion function and cyclic AMP reduces LFA-1 adhesion without affecting CD2-LFA-3 interactions. Up-regulation of the LFA-1 pathway occurs in the absence of any detectable surface redistribution of this molecule, suggesting an activation dependent modification leading to a high-affinity ICAM-1 binding state. The TcR independence of CD2 adhesion function implies a critical role of the CD2 pathway in initiating cell-cell interactions prior to TcR engagement and LFA-1-ICAM-1 binding and underscores the complementary nature of the CD2 and LFA-1 adhesion pathways during the immune response.
authors.affiliation: Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA 02115.
authors.forename: P E
authors.initials: PE
authors.lastname: Moingeon
authors.forename: J L
authors.initials: JL
authors.lastname: Lucich
authors.forename: C C
authors.initials: CC
authors.lastname: Stebbins
authors.forename: M A
authors.initials: MA
authors.lastname: Recny
authors.forename: B P
authors.initials: BP
authors.lastname: Wallner
authors.forename: S
authors.initials: S
authors.lastname: Koyasu
authors.forename: E L
authors.initials: EL
authors.lastname: Reinherz
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D018801:CD2 Antigens; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D011971:Receptors, Immunologic / Q000502:physiology*; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic