title: Co-localization of the retinoblastoma protein and the Epstein-Barr virus-encoded nuclear antigen EBNA-5.
abstract: A monoclonal antibody (aRB1C1) raised against an Rb fusion protein detects a limited number (4-10) of relatively large intranuclear foci in an EBV-immortalized cord blood cell line (IB4). These domains also bind an anti-EBNA-5 monoclonal antibody. The Rb antibody reactive sites also co-localize with the SV40 T antigen in transformed monkey cells (COS). The nuclear structures stained by aRB1C1 and EBNA-5 antibodies are distinct from the structures detected with antibodies against centromeric proteins and certain snRNP epitopes. EBNA-5/Rb-positive domains do not selectively react with antibodies against the La antigen known to associate with the small EBV-encoded nuclear RNA species designated as the EBERs.
authors.affiliation: Department of Medical Cell Genetics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: W Q
authors.initials: WQ
authors.lastname: Jiang
authors.forename: L
authors.initials: L
authors.lastname: Szekely
authors.forename: V
authors.initials: V
authors.lastname: Wendel-Hansen
authors.forename: N
authors.initials: N
authors.lastname: Ringertz
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: A
authors.initials: A
authors.lastname: Rosén
mesh_terms: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000276:immunology / Q000648:ultrastructure*; D019309:Epstein-Barr Virus Nuclear Antigens; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D016160:Retinoblastoma Protein / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D016160:Retinoblastoma Protein