title: The cytochrome chain of mitochondria exhibits variable H+/e- stoichiometry.
abstract: A study is presented of the ----H+/e- stoichiometry for H+ pumping by the cytochrome chain in isolated rat liver mitochondria under level-flow and steady-state conditions. It is shown that the ----H+/e- stoichiometry for the cytochrome chain varies under the influence of the flow rate and transmembrane delta microH+. The rate-dependence is shown to be associated with cytochrome c oxidase, whose ----H+/e- ratio varies from 0 to 1, whilst the ----H+/e- ratio for the span covered by cytochrome c reductase is invariably 2.
authors.affiliation: Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Papa
authors.forename: N
authors.initials: N
authors.lastname: Capitanio
authors.forename: G
authors.initials: G
authors.lastname: Capitanio
authors.forename: E
authors.initials: E
authors.lastname: De Nitto
authors.forename: M
authors.initials: M
authors.lastname: Minuto
mesh_terms: D000818:Animals; D004579:Electron Transport / Q000502:physiology*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D051381:Rats; D013053:Spectrophotometry; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
chemical_list: D013386:Succinates; D006859:Hydrogen; D019802:Succinic Acid; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV