Establishment and characterization of a cholangiocarcinoma cell line from a Thai patient with intrahepatic bile duct cancer.
A new human cholangiocarcinoma cell line (HuCCA-1) was established from cholangiocarcinoma (CCA) tissue fragments surgically removed from a Thai patient with intrahepatic bile duct cancer. The growth medium used for the primary cell culture was Ham's F12 supplemented with 10% fetal bovine serum (FBS) and 10 ng/ml epithelial growth factor (EGF). Approximately one month later, the cells were subcultured in Ham's F12 supplemented with only 10% FBS. The population doubling time was approximately 55 hr. Staining of the cells for cytokeratin and mucin confirmed that the cells were mucin-secreting tumor of epithelial cell origin. The supernatant fluid secreted a number of non-specific tumor markers including CA125 and traces of MCA and AFP. The ability of the HuCCA-1 cell line to synthesize specific marker that may have potential in the diagnosis of cholangiocarcinoma is now being investigated.
Sirisinha
S
S
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Tengchaisri
T
T
Boonpucknavig
S
S
Prempracha
N
N
Ratanarapee
S
S
Pausawasdi
A
A
D002759:Adenoma, Bile Duct / Q000473:pathology*; D001650:Bile Duct Neoplasms / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D008099:Liver / Q000473:pathology; D008297:Male; D008875:Middle Aged; D013785:Thailand
D014408:Biomarkers, Tumor