Conversion of a single-stranded simian virus 40 (SV40)-based shuttle vector to its double-stranded form does not require the SV40 T antigen in monkey cells.
We have transfected the single-stranded DNA form of the simian virus 40 (SV40)-based shuttle vector pZ189 into CV1P simian cells. Although the strand used did not code for the T antigen, we observed its conversion to a double-stranded DNA form. We deduced that the replication step converting a circular single-stranded DNA to a double-stranded one is independent of the SV40 T antigen in simian cells.
Madzak
C
C
Laboratory of Molecular Genetics, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Sarasin
A
A
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000502:physiology*; D015139:Blotting, Southern; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D000882:Haplorhini; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004247:DNA