title: Immunoquantitation of cytochrome C in cardiac perfusate.
abstract: An indirect three step ELISA has been assessed in order to detect the possible release of cytochrome c, a mitochondrial protein, from isolated and perfused guinea-pig heart. The ELISA described in this study is sufficiently sensitive and accurate to measure extracellular cytochrome c.
authors.affiliation: Dept. of Biochemical Sciences Univ. of Rome La Sapienza.
authors.forename: F
authors.initials: F
authors.lastname: Naro
authors.forename: A
authors.initials: A
authors.lastname: Fazzini
authors.forename: G
authors.initials: G
authors.lastname: Citro
authors.forename: F
authors.initials: F
authors.lastname: Malatesta
authors.forename: G
authors.initials: G
authors.lastname: Antonini
authors.forename: P
authors.initials: P
authors.lastname: Sarti
authors.forename: M
authors.initials: M
authors.lastname: Brunori
authors.forename: F
authors.initials: F
authors.lastname: Franconi
authors.forename: A
authors.initials: A
authors.lastname: Giotti
mesh_terms: D000818:Animals; D003574:Cytochrome c Group / Q000032:analysis* / Q000378:metabolism / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D010477:Perfusion; D012015:Reference Standards; D012680:Sensitivity and Specificity
chemical_list: D003574:Cytochrome c Group