T cell receptor beta-chain usage in experimental autoimmune uveoretinitis.
Genomic rearrangements to the T-cell receptor (TCR) V beta 8 gene locus were examined in T cells derived from the lymph nodes of Lewis rats immunized with either S-Antigen or peptides derived from interphotoreceptor retinoid binding protein (IRBP). The cells used in these studies are from T-cell lines that have been selected by several cycles of antigen/IL-2 stimulations, or clones isolated from these lines. No apparent rearrangement of the V beta 8 gene was observed by Southern analysis, suggesting that if indeed there are T cells using V beta 8 gene elements they represent small proportions of the cells in these T-cell lines that induce EAU (uveitogenic T cells) and that the lines may consist of large numbers of clones. On the other hand, we have demonstrated V beta 8 gene expression in uveitogenic T-cell populations by Northern analysis and by polymerase chain reaction (PCR). Although V beta 8 gene transcripts were detectable in pathogenic, but not in non-pathogenic, T-cell lines using a V beta 8 cDNA probe, RNA from pathogenic T cell lines did not hybridize to another probe specific for rat V beta 8.2. Taken together, these results suggest that, unlike the T-cell lines that mediate experimental allergic encephalomyelitis (EAE), some T-cell lines that induce EAU do not predominantly express V beta 8.2 gene but other member(s) of the V beta 8 family.
Egwuagu
C E
CE
Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
Chow
C
C
Beraud
E
E
Caspi
R R
RR
Mahdi
R M
RM
Brézin
A P
AP
Nussenblatt
R B
RB
Gery
I
I
D000818:Animals; D000941:Antigens / Q000276:immunology / Q000633:toxicity; D019393:Arrestin; D001483:Base Sequence; D002460:Cell Line; D005136:Eye Proteins / Q000276:immunology / Q000633:toxicity; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D007114:Immunization; D016219:Immunotherapy, Adoptive; D008969:Molecular Sequence Data; D005810:Multigene Family; D051381:Rats; D011917:Rats, Inbred Lew / Q000235:genetics / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D012177:Retinol-Binding Proteins / Q000276:immunology / Q000633:toxicity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000637:transplantation; D015866:Uveitis, Posterior / Q000209:etiology / Q000235:genetics / Q000276:immunology*; D000099214:Retinol-Binding Proteins, Interstitial
D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial