Localization of major neutralizing epitopes on the S1 polypeptide of the murine coronavirus peplomer glycoprotein.
A recombinant baculovirus system has been used to express the amino terminal half of the murine coronavirus (JHMV) peplomer glycoprotein in insect cells. The expressed polypeptide is glycosylated and is recognized by a set of monoclonal antibodies (mAbs) specific for JHMV S protein. Three of these mAbs have a very high neutralizing activity for JHMV but not for other MHV strains. These results indicate that JHMV-specific, major neutralizing epitopes reside in the amino terminal S1 subunit of the peplomer glycoprotein.
Takase-Yoden
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National Institute of Neuroscience, NCNP, Tokyo, Japan.
Kikuchi
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Siddell
S G
SG
Taguchi
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F
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology*; D007313:Insecta; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D016056:Immunodominant Epitopes; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C064484:spike protein S, Transmissible gastroenteritis virus