Identification of 3',5'-cyclic AMP in axenic cultures of Lemna paucicostata by high-performance liquid chromatography.
Nucleotides were purified from the extract of aseptically grown Lemna paucicostata 6746 by low-pressure column chromatography and thin-layer chromatography. In this partially purified sample, a compound has been identified, using a highly sensitive HPLC-fluorometric detection method, with chromatographic properties and substrate specificity for cyclic nucleotide phosphodiesterase similar to authentic 3',5'-cyclic AMP. The level of cAMP has been estimated to be 70-80 pmol/g fresh wt.
Gangwani
L
L
Department of Plant Molecular Biology, University of Delhi, India.
Tamot
B K
BK
Khurana
J P
JP
Maheshwari
S C
SC
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000242:Cyclic AMP / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014316:Tritium
D014316:Tritium; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases