title: Further characterization of ts1-8, a mutant of herpes simplex virus type 1.
abstract: Our initial characterization of a herpes simplex virus type 1, temperature sensitive host shutoff mutant, called ts1-8, revealed that it has a low plaquing efficiency and exhibits a defect in the shutoff of host polypeptide synthesis and host DNA replication at the nonpermissive temperature of 39.5 degrees C. Using intratypic marker rescue experiments the ts plaquing mutation was mapped to a 557 bp region. Sequence analysis and complementation studies revealed that the low plaquing efficiency phenotype is due to a mutation in the glycoprotein B gene converting Pro-357 to Ser. This novel tsgB mutation is located in a conserved region of gB and it is distinct from the delayed host shutoff mutation (dhs).
authors.affiliation: Department of Microbiology, University of Toronto, Ontario, Canada.
authors.forename: E F
authors.initials: EF
authors.lastname: Arko
authors.forename: J I
authors.initials: JI
authors.lastname: Daksis
authors.forename: V L
authors.initials: VL
authors.lastname: Chan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004261:DNA Replication / Q000235:genetics; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation; D018139:Simplexvirus / Q000235:genetics*; D013696:Temperature; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus