A novel gamma subunit of the GABAA receptor identified using the polymerase chain reaction.
We have utilized a polymerase chain reaction (PCR) strategy to identify a novel subunit, gamma 3, of the GABAA receptor. The gamma 3 cDNA encodes a mature protein of 450 amino acids that contains structural features typically conserved among subunits of the GABAA receptor family. The gamma 3 subunit shares approximately 66% sequence identity with the gamma 2 subunit but only 38% and 29% with alpha 1 and beta 1 subunits, respectively. Localization of the gamma 3 mRNA indicates that it is widely distributed throughout the mouse brain in a pattern similar to that observed for mRNAs encoding the gamma 2 subunits.
Wilson-Shaw
D
D
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
Robinson
M
M
Gambarana
C
C
Siegel
R E
RE
Sikela
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D011963:Receptors, GABA-A / Q000737:chemistry* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
D046911:Macromolecular Substances; D012333:RNA, Messenger; D011963:Receptors, GABA-A