title: Cloning, pharmacological characteristics and expression pattern of the rat GABAA receptor alpha 4 subunit.
abstract: A cDNA of rat brain encoding the GABAA receptor alpha 4 subunit has been cloned. Recombinant receptors composed of alpha 4, beta 2 and gamma 2 subunit bind with high affinity the GABA agonist [3H]muscimol and the benzodiazepine 'alcohol antagonist' [3H]Ro 15-4513, but fail to bind benzodiazepine agonists. The alpha 4 subunit is expressed mainly in the thalamus, as assessed by in situ hybridization histochemistry, and may participate in a major population of thalamic GABAA receptors. The alpha 4 mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum.
authors.affiliation: Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Heidelberg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Wisden
authors.forename: A
authors.initials: A
authors.lastname: Herb
authors.forename: H
authors.initials: H
authors.lastname: Wieland
authors.forename: K
authors.initials: K
authors.lastname: Keinänen
authors.forename: H
authors.initials: H
authors.lastname: Lüddens
authors.forename: P H
authors.initials: PH
authors.lastname: Seeburg
mesh_terms: D000345:Affinity Labels / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001386:Azides / Q000378:metabolism; D001483:Base Sequence; D001569:Benzodiazepines / Q000378:metabolism; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009118:Muscimol / Q000378:metabolism; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011963:Receptors, GABA-A / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism
chemical_list: D000345:Affinity Labels; D001386:Azides; C546262:Gabra4 protein, rat; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D011994:Recombinant Proteins; D001569:Benzodiazepines; D009118:Muscimol; C042957:Ro 15-4513