Ca2+/calmodulin-dependent protein kinase II immunoreactivity in Lewy bodies.
Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) is one of the predominant protein kinases in the brain. We found that CaM kinase II immunoreactivity was concentrated in the peripheral halos of Lewy bodies (LBs) in Parkinson's disease and Lewy body-like hyaline inclusions (LBHIs) in amyotrophic lateral sclerosis. An immunoelectron microscopic examination of LBs revealed that the filaments at the periphery of LBs were decorated with immunopositive deposits. Since CaM kinase II has a broad substrate specificity and can phosphorylate neurofilaments and other cytoskeletal proteins, it may play some role in the formation of LBs and LBHIs through the aberrant phosphorylation of the cytoskeletal elements in these inclusions.
Iwatsubo
T
T
Department of Neuropathology, Faculty of Medicine, University of Tokyo, Japan.
Nakano
I
I
Fukunaga
K
K
Miyamoto
E
E
D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D016631:Lewy Bodies / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D010300:Parkinson Disease / Q000201:enzymology* / Q000473:pathology; D011494:Protein Kinases / Q000032:analysis*; D012016:Reference Values; D013116:Spinal Cord / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure
D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases