Regulation of prostaglandin E2 receptor binding activity in porcine temporal cortex by protein phosphorylation.
Regulation of prostaglandin (PG) E2 receptors was investigated in a 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate-solubilized fraction from the synaptic membrane of porcine temporal cortex. The fraction was preincubated with exogenous protein kinases, and then the binding of PGE2 was measured. PGE2 binding was increased approximately twofold by pretreatment with the catalytic subunit of cyclic AMP-dependent protein kinase (A kinase) or calmodulin-dependent protein kinase II but not by that with protein kinase C. The increase was dependent on the ATP concentration, with ED50 values being close to the Km values of these protein kinases. Protein kinase inhibitors specific for A kinase and for calmodulin-dependent protein kinase II abolished the effect in a dose-dependent manner, with IC50 values being similar to those reported. Further study using the catalytic subunit of A kinase revealed that the maximal binding capacity apparently increased without affecting the affinity and the rate constants for association and dissociation. On the other hand, acid phosphatase treatment reduced the binding activity to the level of nonspecific binding. In addition, treatment by A kinase did not affect the binding of guanosine 5'-(3-thiotriphosphate) by the GTP-binding proteins and the activation of adenylate cyclase mediated by stimulatory guanine nucleotide-binding regulatory protein, and therefore the phosphorylation is believed to occur on the receptor protein. The results suggest that the PGE2 receptor can take active phosphorylated and inactive dephosphorylated forms, of which only the phosphorylated one can bind PGE2.
Morii
H
H
Department of Neuroscience, Osaka Bioscience Institute, Japan.
Tanemura
M
M
Watanabe
Y
Y
D000135:Acid Phosphatase / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D019204:GTP-Binding Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011458:Prostaglandins E / Q000378:metabolism; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000494:pharmacology; D011982:Receptors, Prostaglandin / Q000378:metabolism*; D018078:Receptors, Prostaglandin E; D013223:Statistics as Topic; D013552:Swine; D013702:Temporal Lobe / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D011458:Prostaglandins E; D047428:Protein Kinase Inhibitors; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D000135:Acid Phosphatase; D019204:GTP-Binding Proteins