Inhibition of glucose production during hepatic nerve stimulation in regenerating rat liver perfused in situ. Possible involvement of gap junctions in the action of sympathetic nerves.
To explore the possible role of gap junctions in neural regulation of hepatic glucose metabolism, the effects of hepatic nerve stimulation on metabolic and hemodynamic changes were examined in normal and regenerating rat liver which was perfused in situ at constant pressure via the portal vein with a medium containing 5 mM glucose, 2 mM lactate and 0.2 mM pyruvate. 1. The content of connexin 32, a major component of gap junctions in rat liver, decreased transiently to about 25% of the control level in regenerating liver 48-72 h after partial hepatectomy and recovered to normal by the 11th day after the operation. 2. In normal liver, electrical stimulation of the hepatic nerves (10 Hz, 20 V, 2 ms) and infusion of noradrenaline (1 microM) both increased glucose and lactate output and reduced perfusion flow. 3. In early stage of regenerating liver 48 h and 72 h after partial hepatectomy, the increase in glucose output in response to nerve stimulation was almost completely inhibited, whereas the change in lactate balance was partially suppressed and the reduction of flow rate was retained. The response of glucose output to nerve stimulation recovered by the 11th day after partial hepatectomy. In contrast, exogenous application of noradrenaline increased glucose output even in the early stage of regenerating liver. 4. The increase in noradrenaline overflow during hepatic nerve stimulation in the early stage of regenerating liver was approximately the same as in normal liver. Liver glycogen was sufficiently preserved in the early stage of regenerating liver. However, noradrenaline infusion could no more increase glucose output both in normal and in regenerating livers after 24 h of fasting that depleted liver glycogen. These results suggest that the impaired effects of sympathetic nerve stimulation on glucose metabolism observed in regenerating liver are derived neither from reduced release of noradrenaline nor from depletion of liver glycogen, but rather from transient reduction of gap junctions which assist signal propagation of the nerve action through intercellular communication in rat liver.
Iwai
M
M
Department of Medical Biochemistry, Ehime University School of Medicine, Japan.
Miyashita
T
T
Shimazu
T
T
D000818:Animals; D017630:Connexins; D004558:Electric Stimulation; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D006439:Hemodynamics; D006498:Hepatectomy; D007365:Intercellular Junctions / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000294:innervation / Q000378:metabolism*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009638:Norepinephrine / Q000008:administration & dosage; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013564:Sympathetic Nervous System / Q000378:metabolism*
D017630:Connexins; D007773:Lactates; D008565:Membrane Proteins; D006003:Glycogen; D005947:Glucose; D009638:Norepinephrine