Interleukin-1 alpha gene-transcription in murine keratinocytes is inhibited by HSV-1 infection.
The effect of in vitro infection with herpes simplex virus 1 (HSV-1) on the Interleukin-1 (IL-1) activity of murine keratinocytes was investigated. IL-1 alpha mRNA synthesis was measured by the Northern blot technique, and the IL-1 protein production was measured in terms of the ability of dialysed supernatants from cultures of uninfected and HSV-1 infected keratinocytes to enhance mitogen-induced murine thymocyte proliferation. IL-1 alpha mRNA-synthesis in uninfected keratinocytes was detected 24 h and 48 h after isolation of the keratinocytes. IL-1 protein secretion by these keratinocytes was measureable at 18 h and reached a peak of 73 h, whereas intracellular and membrane-bound IL-1 protein production reached a maximum after 25 h. Keratinocytes, which had been cultured in vitro for 18 h, were infected with HSV-1 for 2 h and further cultured for an additional 4 h or 22 h before IL-1 measurements. A marked reduction of IL-1 alpha gene-expression was noted 6 hours after HSV-1-infection of keratinocytes, and nearly total shut-off was detected after infection for 24 h. Reduced gene-expression was paralleled by a reduction in the IL-1 protein secretion from the HSV-1 infected keratinocytes.
Enk
C
C
Department of Molecular Virology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Sprecher
E
E
Becker
Y
Y
D000818:Animals; D002478:Cells, Cultured; D015870:Gene Expression; D007375:Interleukin-1 / Q000235:genetics* / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism* / Q000382:microbiology; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000502:physiology*; D014158:Transcription, Genetic; D014779:Virus Replication
D007375:Interleukin-1