Specific endonucleolytic cleavage of IGF-II mRNAs.
Expression of the human insulin-like growth factor II (IGF-II) gene gives rise to a family of mRNAs which are expressed in a tissue- and developmental-specific manner. Recently, an IGF-II RNA species of 1.8 kb was detected which consists of the 3' terminal untranslated region of the last IGF-II exon. We have investigated the mechanism of formation of this RNA employing IGF-II minigenes. Analysis of the expression of these genes has revealed that the 1.8-kb RNA does not result from transcription activation, but arises by specific cleavage of IGF-II transcripts.
Meinsma
D
D
Wilhelmina Childrens Hospital, Utrecht, The Netherlands.
Holthuizen
P E
PE
Van den Brande
J L
JL
Sussenbach
J S
JS
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002151:Calorimetry; D002460:Cell Line; D003001:Cloning, Molecular; D004720:Endonucleases / Q000378:metabolism*; D005091:Exons; D005796:Genes; D006801:Humans; D007328:Insulin / Q000235:genetics; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D007328:Insulin; D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II; D004720:Endonucleases