Pharmacological modulation of neuropeptides in peripheral mononuclear cells.
The concentrations of beta-endorphin and cholecystokinin were measured in fresh resting peripheral mononuclear cells obtained from rats and human subjects in basal conditions and after different pharmacological treatments. Both in the human and the rat, beta-endorphin concentrations in mononuclear cells, increased after treatment with serotoninergic agonists, decreased after dopaminergic or GABAergic drugs, while the respective antagonists exerted the opposite effect. In vitro, serotoninergic and GABAergic compounds confirmed their roles in the modulation of beta-endorphin in mononuclear cells. Cholecystokinin was never affected by the pharmacological treatments.
Sacerdote
P
P
Department of Pharmacology, School of Medicine, University of Milan, Italy.
Rubboli
F
F
Locatelli
L
L
Ciciliato
I
I
Mantegazza
P
P
Panerai
A E
AE
D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015259:Dopamine Agents / Q000494:pharmacology; D006801:Humans; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000502:physiology; D001615:beta-Endorphin / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000502:physiology
D015259:Dopamine Agents; D012701:Serotonin; D005680:gamma-Aminobutyric Acid; D001615:beta-Endorphin; D002766:Cholecystokinin