Kinetics of bactericidal activity of antibiotics measured by luciferin-luciferase assay.
ATP production, measured by the luciferin-luciferase assay, is an indicator of bacterial metabolic activity. This enzymatic assay yields rapid results (less than 5 minutes), permitting multiple measurements and establishment of ATP growth curves in order to study the kinetics of antibiotics in bacterial populations. The measurement of free or extracellular ATP, total ATP (extra and intracellular) and the ratio of free to total ATP are additional means of studying the bacteriostatic or bactericidal activity of antibiotics. An increase in free ATP is an indicator of extracellular movement due to alteration of the cell wall. The ratio free ATP/total ATP x 100 greater than or equal to 50%, indicates bacteriallysis. These assays were used to study the effects of 14 antibiotics on two reference strains of Escherichia coli ATCC 25922 and Staphylococcus aureus 25923.
de Rautlin de la Roy
Y
Y
Laboratoire de Microbiology A, Centre Hospitalier Régional, La Milétrie, Poitiers, France.
Messedi
N
N
Grollier
G
G
Grignon
B
B
D000255:Adenosine Triphosphate / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D001681:Biological Assay; D002462:Cell Membrane / Q000187:drug effects; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D049454:Firefly Luciferin; D007700:Kinetics; D008156:Luciferases; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D059005:Topoisomerase II Inhibitors
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D059005:Topoisomerase II Inhibitors; D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D008156:Luciferases; D012321:DNA-Directed RNA Polymerases