[Human B-lymphoblastoid cell lines: optimal conditions for their procurement, selection, cultivation and cloning].
The protocol of Epstein-Barr virus (EBV)-induced transformation of B-lymphocytes from reripheral blood of normal humans has been described. The requirements for mononuclear EBV-infection and for selection, cultivation, and cloning of EBV-transformed cell lines have been investigated. Successful establishment of stable human immunoglobulin-producing cell lines from lymphocytes of immunized donors could be achieved following the protocol presented.
Deriugina
E I
EI
Olovnikova
N I
NI
Lemeneva
L N
LN
Drize
N I
NI
Belkina
E V
EV
Chertkov
I L
IL
D001402:B-Lymphocytes / Q000166:cytology* / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000502:physiology*; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology; D003584:Cytological Techniques; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques
B-limfoblastoidnye kletochnye linii cheloveka: optimizatsiia usloviĭ polucheniia, selektsii, kul'tivirovaniia i klonirovaniia.