Characterization of a new human multiple myeloma cell line, UMJF-2, which suppresses antibody production by B-lymphocytes in vitro.
A new human plasma cell line, UMJF-2, has been derived from the bone marrow of a patient with multiple myeloma. Morphological studies disclosed large nucleoli, moderate numbers of mitochondria, and scant endoplasmic reticulum consistent with a plasmablastic morphology. The cells have immunologic characteristics of early plasma cells, including intense expression of cytoplasmic IgG-lambda and weaker, but discernible, expression of surface IgG-lambda. Cell surface antigens defined by the monoclonal antibodies OKT10 (CD38) and PCA-1, characteristic of mature plasma cells, and B1 (CD20), B4 (CD19), and I-2 (HLA-DR), characteristic of earlier stages of B-lymphocyte differentiation, are present on UMJF-2 cells. Cytogenetic studies reveal the presence of trisomy 12. UMJF-2 does not contain the Epstein-Barr virus by Southern blot analysis. Tissue culture media conditioned by these cells contains a soluble immunosuppressive factor, capable of inhibiting pokeweed mitogen induced IgM secretion by normal human B-lymphocytes. UMJF-2 provides a model for the study of the pathogenesis of polyclonal hypogammaglobulinemia in human multiple myeloma.
Farnen
J P
JP
Department of Internal Medicine, University of Michigan, Ann Arbor.
Tyrkus
M
M
Hanson
C A
CA
Cody
R L
RL
Emerson
S G
SG
D000368:Aged; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism*; D016130:Immunophenotyping; D007621:Karyotyping; D008297:Male; D009101:Multiple Myeloma / Q000235:genetics / Q000276:immunology / Q000473:pathology
D000954:Antigens, Surface; D007075:Immunoglobulin M; C033027:secretory IgM