Hydrolysis of nucleic acids in single-cell protein concentrates using immobilized benzonase.
Hydrolysis of nucleic acids for single-cell protein concentrates has been carried out in one step using immobilized benzonase on corn cob. The immobilization is carried out by tosylation of primary alcohols of cellulose of corn cob. The immobilized benzonase is more stable vs pH changes than native benzonase, but the same optimum values of [Mg(II)] and temperature are obtained. The DNase activity is greater than the RNase activity. The percentage of DNA is reduced to 3-6% and that of RNA to 50%. The protein loss is negligible (1%). The enzymatic activity per weight unit of enzyme is greater in the case of benzonase that in reported data for other nucleases insolubilized on corn cob by the same procedure.
Moreno
J M
JM
Department of Organic and Pharmaceutical Chemistry, Faculty of Pharmacy, Universidad Complutense, Madrid, Spain.
Sanchez-Montero
J M
JM
Ballesteros
A
A
Sinisterra
J V
JV
D004271:DNA, Fungal / Q000378:metabolism*; D004720:Endonucleases / Q000494:pharmacology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012331:RNA, Fungal / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D004271:DNA, Fungal; D004800:Enzymes, Immobilized; D012331:RNA, Fungal; D004720:Endonucleases