Temporal expression of neurofilament polypeptides in differentiating neuroblastoma cells.
NEURO-2A neuroblastoma cells were differentiated by the addition of dibutyryl-cyclic AMP, resulting in an increase in transcription of mRNA coding for the two neurofilament polypeptides NF-L and NF-M. The mRNAs for these two polypeptides appeared to be co-expressed with both being switched on at 24 h after the addition of drug. This was in contrast to NF-H whose induction was only seen at 60 h following addition of drug. These results are in good agreement with the expression time course previously reported in-vivo and suggest that the neurofilament triplet polypeptides can be divided into two subsets which appear to be under different genetic control mechanisms according to their time course of expression.
Breen
K C
KC
Dept. of Immunology, St. Georges Hospital Medical School, London, UK.
Anderton
B H
BH
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D015870:Gene Expression; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016900:Neurofilament Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D016900:Neurofilament Proteins; D012333:RNA, Messenger; C044882:neurofilament protein L; C051774:neurofilament protein H; C061195:neurofilament protein M; D003994:Bucladesine