title: [A nonisotopic enzymolysis assay method of phosphatidylinositol kinase].
abstract: A nonisotopic enzymolysis assay method of phosphatidylinositol kinase (PIK) has been developed. Phosphatidylinositol 4-phosphate (PIP), phosphorylated from phosphatidylinositol (PI) by PIK was hydrolyzed with phosphainositide-specific phospholipase C. The product, inositol 1, 4-bisphosphate (IP2), was separated from inositol 1-phosphate (IP) with Dowex-1 column chromatography. Then, the IP2 was hydrolyzed by alkaline phosphatase to yield PI, and the PI was determined by colorimetry. The PIK activity was defined as PI nmol/mg protein per min. The recovery was 91%, CV = 6.5%
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: I
authors.initials: I
authors.lastname: Ma
mesh_terms: D019870:1-Phosphatidylinositol 4-Kinase; D002845:Chromatography / Q000379:methods; D006801:Humans; D010770:Phosphotransferases / Q000378:metabolism*
chemical_list: D010770:Phosphotransferases; D019870:1-Phosphatidylinositol 4-Kinase