Incorporation into liver microsomal lipids of linoleic and stearic acids and of their respective products of delta 6 and delta 9 desaturation, gamma-linolenic and oleic acids: effect of age and of blackcurrant seed oil.
The incorporation of [1-14C]linoleic and [1-14C]stearic acid and of their delta 6 and delta 9 desaturation products (gamma-linolenic and oleic acids, respectively) into different classes of lipids was studied in liver microsomes of rats in function of the diet (blackcurrant seed oil diet, containing gamma-linolenic acid, versus control diet) and in function of age (3, 6 and 9 months). After delta 6 desaturation, total radioactivity was distributed between phospholipids, especially phosphatidylcholine, and neutral lipids. The desaturation product, gamma-linolenic acid, was totally recovered in the phospholipid fraction. Blackcurrant seed oil, which decreased the rate of delta 6 desaturation in 6- and 9-month-old rats, also decreased the incorporation of radioactivity in total phospholipids, especially in phosphatidylcholine. At 6 months of age, after delta 9 desaturation, the majority of radioactivity was recovered in neutral lipids principally as oleic acid, the desaturation product. The precursor, stearic acid, was highly incorporated into phospholipids, especially in rats on a diet of blackcurrant seed oil.
Ulmann
L
L
Unité de Recherche de Nutrition cellulaire et Métabolique, Université de Bourgogne, Dijon, France.
Poisson
J P
JP
Blond
J P
JP
Bézard
J
J
D000375:Aging / Q000378:metabolism*; D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D008042:Linolenic Acids / Q000378:metabolism; D050602:Linoleoyl-CoA Desaturase; D050356:Lipid Metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013229:Stearic Acids / Q000378:metabolism*; D013230:Stearoyl-CoA Desaturase; D017965:gamma-Linolenic Acid
D004042:Dietary Fats, Unsaturated; D008041:Linoleic Acids; D008042:Linolenic Acids; D009829:Oleic Acids; D010938:Plant Oils; D013229:Stearic Acids; D019301:Oleic Acid; C031183:stearic acid; D017965:gamma-Linolenic Acid; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; D050602:Linoleoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase