Transplants of fetal substantia nigra regulate glutamic acid decarboxylase gene expression in host striatal neurons.
Lesions of the dopaminergic innervation to the striatum result in increased activity of glutamic acid decarboxylase (GAD) and increased GAD mRNA in striatal GABAergic neurons. Here we show that solid transplants of dopamine-containing fetal mesencephalic tissue placed adjacent to the striatum can completely reverse the elevation of GAD mRNA in the striatum of adult rats with complete lesions of the nigrostriatal dopamine projections. The ability of the fetal transplants to re-establish control over gene expression in host target neurons indicates that there is a significant transneuronal influence of the transplanted neurons. Furthermore, striatal GAD mRNA levels appear to be a good marker of the functional impact of dopamine-producing transplants.
Segovia
J
J
Department of Pharmacology, Georgetown University Medical Center, Washington, DC 20007.
Castro
R
R
Notario
V
V
Gale
K
K
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology; D016380:Brain Tissue Transplantation / Q000502:physiology*; D003342:Corpus Striatum / Q000201:enzymology*; D016332:Fetal Tissue Transplantation / Q000502:physiology; D007839:Functional Laterality; D015971:Gene Expression Regulation, Enzymologic; D005968:Glutamate Decarboxylase / Q000235:genetics*; D008297:Male; D009474:Neurons / Q000201:enzymology*; D016627:Oxidopamine / Q000633:toxicity; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013378:Substantia Nigra / Q000637:transplantation*
D012333:RNA, Messenger; D016627:Oxidopamine; D005968:Glutamate Decarboxylase