Deoxyribonucleic acid strand break induced by the hydroxy radical-generating cyclic peroxide, 4-ethoxy-1,4-dihydro-2,3-benzodioxin-1-ol.
Deoxyribonucleic acid (DNA) strand break caused by a synthetic cyclic peroxide, 4-ethoxy-1,4-dihydro-2,3-benzodioxin-1-ol (Bd) was studied by both ethidium bromide fluorescence quenching and agarose gel electrophoresis. The Bd-mediated DNA strand break occurred dependently on temperature and also on Bd concentration. The reaction proceeded at a temperature higher than 30 degrees C (decomposition temperature of Bd), indicating that the reactive species generated by Bd-decomposition are responsible for the reaction. The reaction was protected by 1,4-diazabicyclo[2.2.2]octane and NaN3, and also moderately by OH radical scavengers such as Na-benzoate and NaBr. Possible involvement of active oxygen radicals including OH radical in the DNA strand break is discussed in relation to the mechanism of Bd decomposition.
Kayamori
N
N
Department of Radiochemistry-Biophysics, Niigata College of Pharmacy, Japan.
Shirota
I
I
Konishi
T
T
Matsugo
S
S
D010582:Bacteriophage lambda / Q000378:metabolism; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004279:DNA, Viral / Q000187:drug effects; D004146:Dioxanes / Q000494:pharmacology*; D005609:Free Radicals; D006878:Hydroxides / Q000494:pharmacology*; D017665:Hydroxyl Radical
D004279:DNA, Viral; D004146:Dioxanes; D005609:Free Radicals; D006878:Hydroxides; D017665:Hydroxyl Radical; C057795:4-ethoxy-1,4-dihydro-2,3-benzodioxin-1-ol; D004247:DNA