Cultivation and characterization of novel human group A rotaviruses with long RNA electropherotypes, subgroup II specificities, and serotype 2 VP7 genes.
During an epidemiological study of human rotavirus infections in Bangladesh, three group A strains hybridized with a serotype 2 oligonucleotide probe, but they had long RNA electropherotypes. The three strains were collected from 8- to 20-month-old infants with acute diarrhea and moderate malnutrition. By a modified isolation procedure, two strains (T-B and T-C) were adapted in MA104 cell cultures. They were identified to be subgroup II specific by enzyme-linked immunosorbent assay with subgroup I- and II-specific monoclonal antibodies and were identified by a fluorescent focus reduction neutralization assay with hyperimmune antisera to be serotype 2 specific. Further characterization of these unusual rotavirus strains needs to be carried out.
Bingnan
F
F
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
Unicomb
L E
LE
Tu
G L
GL
Ali
A
A
Malek
A
A
Rahim
Z
Z
Tzipori
S
S
D000914:Antibodies, Viral; D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics / Q000302:isolation & purification; D036022:Capsid Proteins; D003967:Diarrhea / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D006801:Humans; D007223:Infant; D012367:RNA, Viral / Q000302:isolation & purification; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000382:microbiology; D012703:Serotyping; D014776:Virus Cultivation
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C050779:VP7 protein, Rotavirus