Effects of crocetin on the hepatotoxicity and hepatic DNA binding of aflatoxin B1 in rats.
The effects of crocetin pretreatment on both hepatic aflatoxin B1 (AFB1)-DNA binding and AFB1 hepatotoxicity in rats has been examined. For these studies, male Wistar rats were treated with AFB1 (2 mg/kg) by i.p. administration, and the different degrees of hepatic damage were revealed by the elevations of levels of serum marker enzymes such as aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase and gamma-glutamyltranspeptidase. After pretreatment of the animals with crocetin (2 or 6 mg/kg) daily for three consecutive days, the enzyme elevations were significantly suppressed. This suggested that the crocetin possessed chemopreventive effects on the early acute hepatic damage induced by AFB1. Under these experimental conditions, consistent elevations of hepatic glutathiones (GSH) and activities of glutathione S-transferase (GST) and glutathione peroxidase (GSH-Px) were observed. Crocetin treatment also decreased AFB1-DNA adduct formation in AFB1-treated animals. From these results, we suggest that the protective effect of crocetin on AFB1 hepatotoxicity in rats might be due to the hepatic tissues' defense mechanisms that elevated the cytosol GSH and the activities of GST and GSH-Px.
Wang
C J
CJ
Department of Biochemistry, Chung-Shan Medical and Dental College, Taichung, Taiwan, Republic of China.
Shiow
S J
SJ
Lin
J K
JK
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D002338:Carotenoids / Q000494:pharmacology*; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014801:Vitamin A / Q000031:analogs & derivatives; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D000348:Aflatoxins; D002273:Carcinogens; C487773:trans-sodium crocetinate; D014801:Vitamin A; D002338:Carotenoids; D004247:DNA; D016604:Aflatoxin B1; D005723:gamma-Glutamyltransferase; D005978:Glutathione