title: Developmental profiles of arylsulfatases A and B in rat cerebral cortex and spinal cord.
abstract: Arylsulfatases A (EC 3.1.6.1) and B (EC 3.1.6.12) are lysosomal enzymes that can remove sulfate groups from sulfatides and sulfo-glycosaminoglycans, respectively. The activities of these enzymes in cerebral cortex and in spinal cord of developing rat pups were measured. The tissues were homogenized and the arylsulfatases A and B in the soluble fraction were separated from each other by anion exchange chromatography on DE-52 cellulose. Subsequently, the enzyme activities were assayed with p-nitrocatechol sulfate as substrate at 37 degrees C and pH 5.6. We observed a developmental profile of arylsulfatase A, similar to that previously reported for cerebroside sulfatase (EC 3.1.6.8; (Van der Pal et al. (1990) Biochim. Biophys. Acta 1043, 91-96]. The activity of arylsulfatase A increased gradually during development, whereas arylsulfatase B rose more steeply, peaked around day 15 and declined thereafter. As a consequence the ratio between B and A forms of arylsulfatase dropped from about 4 in 1-week-old pups to 2.2 (cortex) and 0.7 (cord) in 7-week-old rat pups.
authors.affiliation: Laboratory of Veterinary Biochemistry, Utrecht University, The Netherlands.
authors.forename: R H
authors.initials: RH
authors.lastname: van der Pal
authors.forename: W
authors.initials: W
authors.lastname: Klein
authors.forename: L M
authors.initials: LM
authors.lastname: van Golde
authors.forename: M
authors.initials: M
authors.lastname: Lopes-Cardozo
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology*; D002553:Cerebroside-Sulfatase / Q000378:metabolism*; D002803:Chondro-4-Sulfatase / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D005260:Female; D011247:Pregnancy; D051381:Rats; D013116:Spinal Cord / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase