Cloning and characterization of a receptor-class phosphotyrosine phosphatase gene expressed on central nervous system axons in Drosophila melanogaster.
We have cloned and characterized cDNAs coding for a receptor-class phosphotyrosine phosphatase gene from Drosophila melanogaster. The gene maps to the polytene chromosome bands 99A7-8. The cDNA clones code for a polypeptide of 1301 amino acids with a predicted molecular mass of 145 kDa. The extracellular domain includes two fibronectin-type III-like domains. The cytoplasmic region contains two tandemly repeated phosphotyrosine phosphatase-like domains. Residues shown crucial for catalytic activity are absent in the second domain. This Drosophila receptor-class phosphotyrosine phosphatase polypeptide is expressed on axons of the embryonic central nervous system.
Hariharan
I K
IK
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Chuang
P T
PT
Rubin
G M
GM
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001369:Axons / Q000201:enzymology*; D001483:Base Sequence; D002871:Chromosome Banding; D003001:Cloning, Molecular; D015342:DNA Probes; D004331:Drosophila melanogaster / Q000196:embryology / Q000201:enzymology / Q000235:genetics*; D005353:Fibronectins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009420:Nervous System / Q000201:enzymology; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000302:isolation & purification; D011956:Receptors, Cell Surface / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000906:Antibodies; D015342:DNA Probes; D005353:Fibronectins; D009838:Oligodeoxyribonucleotides; D011956:Receptors, Cell Surface; D017027:Protein Tyrosine Phosphatases