title: Establishment of reference methods for lipids, lipoproteins and apolipoproteins.
abstract: Reference methods for lipids, lipoproteins, and apolipoproteins have been developed for use as part of an accuracy base for institutional, national, or international reference systems. A widely accepted reference method exists only for total cholesterol. Well described interim or institutional in-house reference methods have been established for the other lipids, lipoproteins, and apolipoproteins. The major criteria for a reference method are 1) scientific basis, 2) sound principles, 3) available calibration and control materials, 4) traceability to a definitive method or a point of reference, and 5) applicability to reference materials that provide traceability to clinical methods and transferability to other reference laboratories. The total cholesterol reference method of the U.S. National Reference System demonstrates how a reference method can be developed and applied. Reference methods now available can lead to an accepted international accuracy base for the clinically useful lipid, lipoprotein, and apolipoprotein measurements.
authors.affiliation: Centers for Disease Control, Atlanta, Georgia.
authors.forename: G R
authors.initials: GR
authors.lastname: Cooper
authors.forename: G L
authors.initials: GL
authors.lastname: Myers
authors.forename: L O
authors.initials: LO
authors.lastname: Henderson
mesh_terms: D016632:Apolipoprotein A-I; D001053:Apolipoproteins / Q000097:blood*; D001054:Apolipoproteins A / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods / Q000592:standards*; D002487:Centers for Disease Control and Prevention, U.S.; D002624:Chemistry, Clinical; D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood; D006801:Humans; D008055:Lipids / Q000097:blood*; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D012015:Reference Standards; D012958:Societies, Scientific; D014280:Triglycerides / Q000097:blood; D014481:United States
chemical_list: D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D001054:Apolipoproteins A; D008076:Cholesterol, HDL; D008055:Lipids; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D014280:Triglycerides; D002784:Cholesterol