[A sensitive enzyme-linked immunosorbent assay (ELISA) for the serological detection of antibodies against the European hog cholera virus].
An ELISA for the detection of antibodies against hog cholera virus (HCV) was developed. The HCV-specific glycoprotein gp53 served as diagnostic antigen after immobilization using a monoclonal capture antibody. Due to the higher affinity of HCV-specific antibodies to the viral gp53, sera cross reacting with bovine viral diarrhea (BVD) virus were discriminated by the slope of the titration curves.
Schagemann
G
G
Institut für Virologie, Tierärztlichen Hochschule, Hannover.
Greiser-Wilke
I
I
Liess
B
B
Moennig
V
V
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D003429:Cross Reactions; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D013552:Swine
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral
Ein empfindlicher Enzyme-linked Immunosorbent Assay (ELISA) zum serologischen Nachweis von Antikörpern gegen das Virus der Europäischen Schweinepest (ESPV).