Structures of the alpha(1-3)-galactose-containing asparagine-linked glycans of a Lewis lung carcinoma cell subline resistant to Aleuria aurantia agglutinin: elucidation by 1H-NMR spectroscopy.
Four bi-antennary glycan fractions of the N-acetyllactosamine-type, derived from a Lewis lung carcinoma (LL2) cell subline resistant to the Aleuria aurantia agglutinin were studied by 400 MHz 1H-NMR spectroscopy. By this method, their antennae were found to be terminated either by alpha(2-3 or 6)-linked N-acetylneuraminic acid or alpha(1-3)-linked galactose residues. The primary structure of glycans of these four glycopeptide or derived oligosaccharide-alditols has been determined in full detail.
Debray
H
H
Laboratoire de Chimie Biologique, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Dus
D
D
Wieruszeski
J M
JM
Strecker
G
G
Montreuil
J
J
D000818:Animals; D001216:Asparagine / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004351:Drug Resistance / Q000502:physiology; D005690:Galactose / Q000032:analysis*; D006020:Glycopeptides / Q000138:chemical synthesis; D037102:Lectins / Q000627:therapeutic use*; D008175:Lung Neoplasms / Q000737:chemistry* / Q000188:drug therapy; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D011522:Protons; D013402:Sugar Alcohols / Q000737:chemistry; D014407:Tumor Cells, Cultured
D006020:Glycopeptides; D037102:Lectins; D009844:Oligosaccharides; D011134:Polysaccharides; D011522:Protons; D013402:Sugar Alcohols; C064549:lectin, Aleuria aurantia; D001216:Asparagine; D005690:Galactose