title: Influence of different sources of injected selenium on certain enzymes, glutathione and adenosylmethionine concentration in buffalo (Bubalus bubalis) calves.
abstract: Sodium selenite and selenomethionine were investigated as possible causative factors for the induction of Degnala disease syndrome in twelve buffalo (Bubalus bubalis) calves divided into three groups of four. Group 1 was the control group and received no additional selenium. Sodium selenite and selenomethionine were given daily as intramuscular injections on a selenium-equivalent basis, with a weekly increment in the dose of 0.05 mg Se/kg live weight from 0.05 to 0.20 mg Se/kg live weight per day, in groups 2 and 3 respectively. Only one animal from group 3 manifested the lesions of Degnala disease. The blood Se concentration and erythrocyte glutathione peroxidase (EC 1.11.1.9; GSH-Px) activity were both greater in groups 2 and 3 than in control group 1. The overall blood Se concentration was 0.22 (SE 0.01), 0.38 (SE 0.12) and 0.77 (SE 0.20) micrograms Se/ml in groups 1 to 3 respectively with corresponding GSH-Px activities of 63.84 (SE 7.38), 88.37 (SE 12.38) and 165.32 (SE 40.62) enzyme units/mg protein. Erythrocyte glutathione reductase (NAD(P)H) (EC 1.6.4.2) activity was not affected by treatment but reduced glutathione content was lower in groups 2 and 3. Liver adenosylmethionine, estimated at autopsy, was lowest (22.87 (SE 6.17) mumol/g) in group 3, and greatest (102.63 (SE 9.39) mumol/g) in group 1 (P less than 0.01). Organic Se sources seemed to accumulate in tissues more than inorganic sources, and might be the causative toxic factors of Degnala disease.
authors.affiliation: National Dairy Research Institute, Haryana, India.
authors.forename: T
authors.initials: T
authors.lastname: Prasad
authors.forename: S P
authors.initials: SP
authors.lastname: Arora
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology*; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007273:Injections, Intramuscular; D008099:Liver / Q000378:metabolism*; D008297:Male; D012436:S-Adenosylmethionine / Q000032:analysis / Q000378:metabolism*; D012643:Selenium / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity*; D012645:Selenomethionine / Q000008:administration & dosage; D018038:Sodium Selenite
chemical_list: D012436:S-Adenosylmethionine; D012645:Selenomethionine; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione; D012643:Selenium; D018038:Sodium Selenite