Stabilization of a miniderivative of the broad-host-range IncW plasmid pSa by insertion of plasmid R1 parB region.
The plasmid vector pEM100 (13.5 kb) constructed from pGV1106, a miniderivative of the broad-host-range IncW pSa plasmid, and the pAM330 plasmid of Brevibacterium lactofermentum is not stably maintained in Escherichia coli host cells under nonselective growth conditions. By insertion of a 0.9 kb DNA fragment containing the parB locus (responsible for the maintenance of plasmid R1 in E. coli cells) to plasmid pEM100, plasmid pEM110 was prepared which is maintained in a population of E. coli cells growing without a selection pressure very stably.
Nesvera
J
J
Department of Molecular Genetics of Bacteria, Czechoslovak Academy of Sciences, Prague.
Hochmannová
J
J
Pátek
M
M
D001951:Brevibacterium / Q000235:genetics; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D005819:Genetic Markers; D011815:R Factors / Q000235:genetics*; D011995:Recombination, Genetic; D013045:Species Specificity
D004251:DNA Transposable Elements; D005819:Genetic Markers