title: [Spectrophotometric method of studying the cleavage of DNA-duplexes by restriction endonucleases].
abstract: A spectrophotometric method for continuous monitoring the cleavage of DNA duplexes by type II restriction endonucleases was proposed. The time course of cleavage of a 14-membered DNA duplex by MvaI endonuclease was obtained. The spectrophotometric method is characterized by rapidity and high precision in determining the kinetic parameters of the reaction. It can be recommended for testing the preparations for the presence of restriction endonucleases, rapid determination of the activity of any restriction endonucleases, highly precise quantitative analysis of the restriction enzyme catalysed reactions.
authors.forename: O V
authors.initials: OV
authors.lastname: Piatrauskene
authors.forename: E A
authors.initials: EA
authors.lastname: Kubareva
authors.forename: E S
authors.initials: ES
authors.lastname: Gromova
mesh_terms: D002384:Catalysis; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D006868:Hydrolysis; D009692:Nucleic Acid Heteroduplexes; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009692:Nucleic Acid Heteroduplexes; D004247:DNA; D004262:DNA Restriction Enzymes
vernacular_title: Spektrofotometricheskiĭ metod izucheniia rasshchepleniia DNK-dupleksov éndonukleazami restriktsii.