title: Inhibition of a cell surface protease after cisplatin chemotherapy.
abstract: The epithelial cells in squamous carcinoma and leukoplakia of the oral cavity possess the cell surface protease, guanidinobenzoatase (GB), in an active form. GB is closely similar to plasminogen activator, a protease associated with both transformed cells and tumour cells. The active centre of GB binds the fluorescent probe 9-aminoacridine (9-AA) enabling cells containing active GB to be visualised by fluorescent microscopy. It was observed that chemotherapy with cisplatin resulted in a marked decrease in cell surface GB activity and this decrease was due to the formation of an enzyme-inhibitor complex. One of the results of chemotherapy was shown to be the suppression of a cell surface protease which is known to be associated with migration and malignancy of cells in vivo.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, UK.
authors.forename: F S
authors.initials: FS
authors.lastname: Steven
authors.forename: J
authors.initials: J
authors.lastname: Zoeller
authors.forename: H
authors.initials: H
authors.lastname: Maier
authors.forename: I A
authors.initials: IA
authors.lastname: Born
mesh_terms: D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D002462:Cell Membrane / Q000201:enzymology*; D002945:Cisplatin / Q000627:therapeutic use*; D010450:Endopeptidases / Q000378:metabolism*; D004848:Epithelium / Q000201:enzymology / Q000473:pathology; D005557:Formaldehyde; D006801:Humans; D007700:Kinetics; D007971:Leukoplakia / Q000201:enzymology / Q000473:pathology; D008856:Microscopy, Fluorescence; D009061:Mouth Mucosa / Q000201:enzymology; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D011480:Protease Inhibitors; D014059:Tongue / Q000201:enzymology
chemical_list: D011480:Protease Inhibitors; D005557:Formaldehyde; D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases; D002945:Cisplatin