Cell cycle perturbating agents in a line of human thyroid transformed cells in culture (HuT).
Culture and differentiation parameters of a human thyroid transformed cell line (HuT) were analyzed. Treatment with high concentrations of chemical agents namely dimethyl sulphoxide and retinoic acid, exerted a dramatic cytotoxic effect. The exposure of these cells to the lowest doses of retinoic acid as well as to 8 mM-16 mM 3-aminobenzamide a potent inhibitor of poly(ADPribose)polymerase, resulted in a delay of cell proliferation. Poly(ADPribose)polymerase activity was differently affected by retinoic acid (stimulation) and 3-aminobenzamide (inhibition).
Mancini
A
A
Dipartimento di Chimica Organica e Biologica, Facoltà di Scienze, Università di Napoli, Italy.
Faraone-Mennella
M R
MR
Quesada
P
P
Malanga
M
M
Farinai
B
B
D001549:Benzamides / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000032:analysis; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008854:Microscopy, Electron; D008875:Middle Aged; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D013539:Simian virus 40 / Q000502:physiology; D013961:Thyroid Gland / Q000166:cytology; D014212:Tretinoin / Q000494:pharmacology*
D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D014212:Tretinoin; C025160:3-aminobenzamide; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D004121:Dimethyl Sulfoxide