The 3'-flanking sequence of the bovine growth hormone gene contains novel elements required for efficient and accurate polyadenylation.
In addition to the conserved AAUAAA hexanucleotide, GU- and U-rich sequences in the 3'-flanking region are thought to be critical for efficient polyadenylation. The 3'-flanking sequence requirements for efficient and accurate polyadenylation of the bovine growth hormone (bGH) gene were determined by quantitative S1 nuclease analysis of transcripts derived from various bGH 3' deletions and block mutations transiently transfected into COS-1 cells. Though the bGH 3'-flanking sequence contains a portion of the putative GU efficiency element, we find that mutation of this element leads to a marginal decrease in efficiency similar to that from mutation of other sequences that do not contain recognizable GU- or U-rich motifs. The data are consistent with a diffuse efficiency element in the bGH polyadenylation signal rather than a discrete element as is thought to exist in other mammalian signals. We have also determined that a region from 18 to 27 nucleotides downstream of the cleavage site contains sequences required for correctly positioning the cleavage site.
Goodwin
E C
EC
Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Rottman
F M
FM
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D002872:Chromosome Deletion; D015342:DNA Probes; D005809:Genes, Regulator; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D011061:Poly A / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic; D014162:Transfection
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; D011061:Poly A; D013006:Growth Hormone