Anti-Ig inactivation of the CH31 lymphoma model for immature B cell inhibits its ability to process pigeon cytochrome c.
We wished to determine whether tolerized cells are able to process and present antigen based on our hypothesis that tolerized B cells be unable to function in the normal capacity as antigen-presenting cells if they are to remain the tolerant state. Results in this study show that the ability of the murine lymphoma model for immature B cells, CH31, to process pigeon cytochrome c was greatly down-regulated when cultured in the presence of rabbit anti-mouse IgM. In contrast, the same anti-IgM treatment had no significant effect on the antigen-presenting cell function of the lymphoma model for mature B cells, CH112. Presentation of CNBr-cleaved fragments of pigeon cytochrome c by either CH31 or CH12 cells was not affected by the antibody treatment. Furthermore, CH31 cells pre-incubated with pigeon cytochrome c were not subject to the anti-IgM inhibition of the antigen presentation. These observations suggest that pertubation of surface immunoglobulin molecules on CH31 immature B cells causes down-regulation of their antigen-processing machinery.
Pinto
V B
VB
Department of Microbiology and Immunology, University of Miami School of Medicine, FL 33101.
Peacock
J S
JS
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002460:Cell Line; D010856:Columbidae; D003574:Cytochrome c Group / Q000378:metabolism*; D004306:Dose-Response Relationship, Immunologic; D015536:Down-Regulation; D005434:Flow Cytometry; D007075:Immunoglobulin M; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D016393:Lymphoma, B-Cell / Q000188:drug therapy* / Q000276:immunology; D051379:Mice
D000888:Antibodies, Anti-Idiotypic; D003574:Cytochrome c Group; D007075:Immunoglobulin M; D007376:Interleukin-2