title: pEXPRESS: a family of expression vectors containing a single transcription unit active in prokaryotes, eukaryotes and in vitro.
abstract: We have constructed a family of expression vectors containing a single transcription unit that is active in Escherichia coli, eukaryotic cells, and in coupled in vitro transcription-translation systems. These vectors use the Rous sarcoma virus-long terminal repeat (RSV-LTR) as the promoter/enhancer for eukaryotic cells. In vitro transcription is made possible by inclusion of a bacteriophage T7 promoter. This same promoter is actively transcribed in E. coli that produce T7 RNA polymerase. Other features of this transcription unit include a high-efficiency eukaryotic translation start codon, a phage f1 origin of DNA replication for site-directed mutagenesis and a three-frame stop codon that facilitates C-terminal deletion mutagenesis. We term this vector family, pEXPRESS.
authors.affiliation: Department of Pharmacology, New York University Medical Center, NY 10016.
authors.forename: B M
authors.initials: BM
authors.lastname: Forman
authors.forename: H H
authors.initials: HH
authors.lastname: Samuels
mesh_terms: D001358:Avian Sarcoma Viruses / Q000235:genetics; D001483:Base Sequence; D002474:Cell-Free System; D003062:Codon / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D011061:Poly A / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011988:Receptors, Thyroid Hormone / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D013604:T-Phages / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D011988:Receptors, Thyroid Hormone; D011994:Recombinant Proteins; D011061:Poly A; D004259:DNA-Directed DNA Polymerase