Spin label detection and free radical nature of DNA damage by hydralazine.
1. Incubation with hydralazine was shown to induce degradative changes of calf thymus DNA spin-labeled with 3-(2-bromoacetamido)-2,2,5,5-tetramethyl-1-pyrrolidono-1-oxyl and 4-(2-bromoacetamido)-2,2,6,6-tetramethylpiperidino-1-oxyl detectable from electron spin resonance specta. 2. Hydralazine, especially in the presence of Fe2+ induced formation of thiobarbituric acid (TBA)-reactive DNA degradation products. 3. The formation of TBA-reactive products was prevented by catalase, EDTA and scavengers of .OH radicals and enhanced by superoxide dismutase which suggests that .OH radicals formed by the Fenton mechanism mediate the DNA damage by hydralazine-Fe2+.
Weglarz
L
L
Department of Biochemistry and Chemistry, Silesian Medical Academy, Katowice, Poland.
Bartosz
G
G
D002374:Catalase / Q000494:pharmacology; D003497:Cyclic N-Oxides; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D004249:DNA Damage; D004492:Edetic Acid / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D006830:Hydralazine / Q000494:pharmacology*; D006878:Hydroxides / Q000378:metabolism; D017665:Hydroxyl Radical; D013113:Spin Labels; D013482:Superoxide Dismutase / Q000494:pharmacology; D013858:Thiobarbiturates
D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D006878:Hydroxides; D013113:Spin Labels; D013858:Thiobarbiturates; C070105:3-(2-bromoacetamido)-2,2,5,5-tetramethyl-1-pyrrolidino-1-oxyl; C028369:tempo 4-bromoacetamide; D006830:Hydralazine; D017665:Hydroxyl Radical; D004247:DNA; D004492:Edetic Acid; D002374:Catalase; D013482:Superoxide Dismutase; C029684:thiobarbituric acid