The cDNA for the heart/muscle isoform of bovine cytochrome c oxidase subunit VIa encodes a presequence.
We have used mixed oligonucleotide probes to isolate a cDNA for the heart/muscle isoform of cytochrome c oxidase (COX) subunit VIa (COX VIa-H) from a bovine heart cDNA library in lambda gt10. This cDNA, and a second one isolated upon rescreening, predict a 97 amino acid COX VIa precursor protein comprised of a 12 amino acid, basic presequence plus an 85 residue mature VIa protein. The presence of a presequence contrasts with the rat heart COX VIa cDNA.
Smith
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Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor 48109-0616.
BeMent
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Grossman
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Lomax
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MI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology*; D011498:Protein Precursors / Q000235:genetics; D051381:Rats
D007527:Isoenzymes; D011498:Protein Precursors; D004247:DNA; D003576:Electron Transport Complex IV