[Monoclonal antibody against human primary hepatocellular carcinoma and immunocytochemical localization of the corresponding antigen].
A monoclonal antibody HAB5 was raised against human hepatocellular carcinoma. The reactivity of HAB5 was determined immuno-histologically on paraffin sections by ABC technique. Positive reaction was seen in 88% of 50 cases of hepatoma examined, including 2 cases of spindle-cell hepatoma. Positive reaction was also seen in 16% squamous-cell carcinoma and 25% transitional cell carcinoma. The monoclonal antibody did not react with metastatic adenocarcinoma of liver in 7 cases. The antigen to which HAB5 directed was a membrane glycoprotein with a molecular weight of 68 KDa.
Ren
F
F
Fourth Military Medical College, Xian, Shanxi.
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D002277:Carcinoma / Q000175:diagnosis; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000175:diagnosis*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm