title: RecA-dependent increased precise excision of Tn10 in Salmonella typhimurium.
abstract: UV irradiation induced the precise excision of Tn10 inserted in met, trp or srl in a Salmonella typhimurium strain; mitomycin C was also found to induce the frequency of precise excision of Tn10 from srl or met. Precise excision of Tn10 was not increased by either UV or mitomycin C in a recA mutant. Similarly, a recA mutant derived from a uvrD strain showed a drastic reduction in the high spontaneous levels of precise excision of Tn10 of this strain. These results indicate that recA is involved in the increased precise excision of Tn10. In contrast to point mutations excision of Tn10 was found to be UV inducible in a top mutant.
authors.affiliation: Cefybo, Buenos Aires, Argentina.
authors.forename: M S
authors.initials: MS
authors.lastname: Levy
authors.forename: P
authors.initials: P
authors.lastname: Pomposiello
authors.forename: R
authors.initials: R
authors.lastname: Nagel
mesh_terms: D002872:Chromosome Deletion; D004260:DNA Repair / Q000187:drug effects / Q000502:physiology*; D004251:DNA Transposable Elements; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D004251:DNA Transposable Elements; D008937:Mitomycins; D016685:Mitomycin; D011938:Rec A Recombinases