title: [Tumor necrosis factor in medicinal interferon preparations].
abstract: In response to virus induction a culture of donor leukocytes alongside with interferon (IF-alpha) produced a factor of tumor necrosis (TNF). The kinetics of TNF and IF-alpha biosynthesis did not depend on the kind of IF used for priming, was rapid, with maximum production within 7-8 hours. Antibodies to IF-alpha and IF-alpha had no effect on TNF production, while antibody to TNF did not reduce IF-alpha yields. TNF in detectable titres was present in medical preparations of native IF-alpha but was absent in preparations of recombinant IF-alpha and IF-alpha as well as in an injection preparation of IF purified by chemical methods.
authors.forename: V P
authors.initials: VP
authors.lastname: Kuznetsov
authors.forename: A A
authors.initials: AA
authors.lastname: Babaiants
authors.forename: I S
authors.initials: IS
authors.lastname: Frolova
authors.forename: L A
authors.initials: LA
authors.lastname: Zueva
authors.forename: D L
authors.initials: DL
authors.lastname: Beliaev
authors.forename: R M
authors.initials: RM
authors.lastname: Khusainov
authors.forename: E V
authors.initials: EV
authors.lastname: D'iakonova
authors.forename: A M
authors.initials: AM
authors.lastname: Vyborov
authors.forename: V V
authors.initials: VV
authors.lastname: Parfenov
mesh_terms: D000906:Antibodies / Q000276:immunology; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004340:Drug Contamination; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D007370:Interferon Type I / Q000032:analysis; D016898:Interferon-alpha / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D009522:Newcastle disease virus; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000472:pathogenicity
chemical_list: D000906:Antibodies; D007369:Interferon Inducers; D007370:Interferon Type I; D016898:Interferon-alpha; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha
vernacular_title: Faktor nekroza opukholi v meditsinskikh preparatakh interferona.