Blood group N antigen precursor glycoproteins and N antigen precursor glycoproteins with Thomsen-Friedenreich (T) activity from human liver metastatic carcinomas.
1. Four perchloric acid-soluble fractions (PASFs) from human liver metastases of sigmoid colon carcinoma (LSCC), pancreas carcinoma (LPC), breast carcinoma (LBC) and human normal liver (NL) were subjected to DEAE-cellulose column chromatography and separated into three glycoprotein fractions, respectively. 2. In chromatography, LSCC-PASF and LBC-PASF gave Thomsen-Friedenreich (T)-active glycoprotein, blood group N antigen precursor glycoprotein with T activity and N antigen precursor glycoprotein, respectively. 3. LPC-PASF gave N antigen precursor glycoprotein with T activity and two N antigen precursor glycoproteins. 4. The three glycoprotein fractions from NL-PASF did not exhibit both T activity and N antigen precursor activity.
Otsuka
K
K
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Kudo
T
T
Nakajima
T
T
Ohkuma
S
S
D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology*; D015295:Antigens, Tumor-Associated, Carbohydrate; D001943:Breast Neoplasms / Q000276:immunology / Q000473:pathology; D002848:Chromatography, DEAE-Cellulose; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology; D004187:Disaccharides / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007519:Isoantigens / Q000276:immunology; D008113:Liver Neoplasms / Q000276:immunology* / Q000556:secondary; D008951:MNSs Blood-Group System / Q000276:immunology*; D010190:Pancreatic Neoplasms / Q000276:immunology / Q000473:pathology; D010472:Perchlorates; D011498:Protein Precursors / Q000276:immunology; D012995:Solubility
D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D004187:Disaccharides; D006023:Glycoproteins; D007519:Isoantigens; D008951:MNSs Blood-Group System; D010472:Perchlorates; D011498:Protein Precursors; C016467:Thomsen-Friedenreich antigen