Clarification of ascites fluid.
An essential first step in the purification of monoclonal antibodies is the clarification of the ascites fluid. While not ignored, this step has not received critical attention. Six different procedures were evaluated with respect to effectiveness and impact on several selected chemical components. For large-scale work, we find an on-line filtration process to be most efficient.
Baum
G
G
Technicon Corporation, Division of Miles, Tarrytown, NY 10591.
Frey
M
M
Santa Teresa
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001202:Ascitic Fluid / Q000737:chemistry*; D002210:Caprylates; D002498:Centrifugation; D005617:Chlorofluorocarbons, Methane; D005374:Filtration / Q000379:methods; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D012822:Silicon Dioxide
D000911:Antibodies, Monoclonal; D002210:Caprylates; D005617:Chlorofluorocarbons, Methane; D012822:Silicon Dioxide; C031492:octanoic acid