[The detection of rotavirus RNA in nasopharyngeal smears by molecular hybridization].
Rotavirus RNA was detected in nasopharyngeal washings, obtained from patients with rotavirus gastroenteritis (76.3% of cases) and gastroenteritis of unknown etiology (34.7% of cases), by the method of molecular hybridization with the use of RNA of monkey virus SA11, labeled with 32P in the process of T4-ligase reaction. In 100% of cases this detection correlated with the presence of lesions of the upper respiratory ways and was probably indicative of the rotavirus nature of the catarrhal syndrome in rotavirus gastroenteritis.
Novikova
N A
NA
Al'tova
E E
EE
Noskova
N V
NV
Epifanova
N V
NV
Tamoĭkina
N A
NA
D000208:Acute Disease; D002648:Child; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000382:microbiology*; D009693:Nucleic Acid Hybridization / Q000235:genetics; D015347:RNA Probes; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D012141:Respiratory Tract Infections / Q000382:microbiology; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*
D015347:RNA Probes; D012367:RNA, Viral
Obnaruzhenie rotavirusnoĭ RNK v nosoglotochnykh smyvakh metodom molekuliarnoĭ gibridizatsii.