[Site-directed mutagenesis with the use of addressed fragmentation of single-stranded DNA for obtaining hybrids of homologous proteins].
Site-directed multiple mutagenesis to obtain hybrids of homologous proteins was carried out by means of the oligonucleotide-targeting digestion of ss DNA. The procedure is more convenient, rapid and simple than the step-by-step approach. To demonstrate different approaches to targeting digestion of ss DNA, NcoI, HinfI, FokI endonucleases were used for DNA cleavage. A hydride of the human and porcine IFN-alpha-2-genes has been constructed.
Shekhter
I I
II
Ratmanova
K I
KI
Veĭko
V P
VP
Debabov
V G
VG
D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D007370:Interferon Type I / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D011994:Recombinant Proteins / Q000138:chemical synthesis / Q000235:genetics; D013552:Swine
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D007370:Interferon Type I; D011994:Recombinant Proteins; D004262:DNA Restriction Enzymes
Saĭt-napravlennyĭ mutagenez s ispol'zovaniem adresovannogo fragmentirovaniia odnonitevoĭ DNK dlia polucheniia gibridov gomologichnykh belkov.