title: Effect of an immunomodulating agent, RU 414740, on polymorphonuclear responsiveness after burn injury.
abstract: An impairment of polymorphonuclear leukocyte (PMN) functions has been described following burn trauma. It was thus of interest to investigate the effect of RU 41740, an agent known to stimulate these cells, on rat PMN functions after burn injury. In the present study the responsiveness to classical stimuli of PMN from untreated burned rats was approximately 40% lower than healthy control values. In vitro treatment with RU 41740 increased oxidative metabolism of PMNs from burned and healthy rats. The effect was dose-related but was most striking in the case of PMNs from healthy rats. Significant differences were obtained with concentrations higher than 1 micrograms/ml for healthy rats but only 10 micrograms/ml for burned rats. In vivo treatment with RU 41740 also led to an enhancement of PMN oxidative metabolism on both burned and healthy rats. The maximal effective dose was 10 mg/kg/day in both cases. In contrast, 25 and 50 mg/kg/day doses inhibited PMN oxidative metabolism.
authors.affiliation: CNRS URA 595-Pharmacologie and Biochimie A Hôpital Cochin, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Roch-Arveiller
authors.forename: J
authors.initials: J
authors.lastname: Fontagne
authors.forename: A
authors.initials: A
authors.lastname: el Abbouyi
authors.forename: D
authors.initials: D
authors.lastname: Raichvarg
authors.forename: J P
authors.initials: JP
authors.lastname: Giroud
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D002056:Burns / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D066298:In Vitro Techniques; D007249:Inflammation / Q000517:prevention & control; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D013481:Superoxides; C037567:glycoprotein, Klebsiella pneumoniae