title: Expression of nicotinic acetylcholine receptor subtypes in brain and retina.
abstract: Neuronal nicotinic acetylcholine receptors (AChRs) are composed of two types of subunits: ACh-binding (termed alpha 2, alpha 3, alpha 4 ...) and structural (termed beta 2, beta 3, beta 4 ...). AChR subtypes composed of combinations of subunits of these two types encoded by several related genes are expressed in different parts of the nervous system, where they presumably serve different functional roles. Here we identify the ACh-binding subunit of the most prominent chicken brain AChR subtype by N-terminal amino acid sequence and show that it corresponds to the alpha 4 gene. Previously we identified the structural subunit for this AChR subtype from chicken brain as beta 2 by N-terminal amino acid sequence. Thus, this identifies both genes which encode subunits of the major nicotinic AChR subtype in avian brains. By immunoprecipitation, immunohistochemistry, and northern blot analysis we show that alpha 3 (or a very closely related sequence) is expressed at low levels in the brain and relatively high levels in the retina, while alpha 4 is expressed at high levels in the brain and lower levels in the retina. This differential expression indicates that alpha 3-containing 'ganglionic-type' AChRs may be an important AChR subtype in avian retina.
authors.affiliation: Salk Institute for Biological Studies, San Diego, CA 92138.
authors.forename: P J
authors.initials: PJ
authors.lastname: Whiting
authors.forename: R
authors.initials: R
authors.lastname: Schoepfer
authors.forename: W G
authors.initials: WG
authors.lastname: Conroy
authors.forename: M J
authors.initials: MJ
authors.lastname: Gore
authors.forename: K T
authors.initials: KT
authors.lastname: Keyser
authors.forename: S
authors.initials: S
authors.lastname: Shimasaki
authors.forename: F
authors.initials: F
authors.lastname: Esch
authors.forename: J M
authors.initials: JM
authors.lastname: Lindstrom
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001923:Brain Chemistry; D002645:Chickens; D005260:Female; D008969:Molecular Sequence Data; D051381:Rats; D011917:Rats, Inbred Lew; D011978:Receptors, Nicotinic / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics; D012160:Retina / Q000737:chemistry*
chemical_list: D011978:Receptors, Nicotinic