Detection of transforming gene regions of human papillomavirus type 16 in cervical dysplasias by the polymerase chain reaction.
In a study of 29 cases of histologically confirmed, characterized colposcopically and cytologically, cervical intraepithelial neoplasias 48.3% (14/29) of biopsies were positive for human papillomavirus (HPV) type 16 DNA by polymerase chain reaction. We used two oligonucleotide primer pairs (position 215-514 and 606-805) flanking a 300 and a 199 base pair fragment from the early 6 (E6) and early 7 (E7) genes. The results were concordant both with the E6 and with the E7 regions. Of the amplified products 85.7% (12/14) could be confirmed; these carried 16 specific sequences by Southern blot hybridization. HPV 16 DNA was present in 6.7% (2/30) of the colposcopically directed cytologically normal matched control samples using the same methods.
Czeglédy
J
J
Institute of Microbiology University Medical School of Debrecen, Hungary.
Evander
M
M
Veres
L
L
Gergely
L
L
Wadell
G
G
D000328:Adult; D001483:Base Sequence; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005814:Genes, Viral; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000382:microbiology
D015344:DNA Probes, HPV; D004279:DNA, Viral