[Studies on antiaggregating drugs of horseshoe crab amebocytes].
The effects of drugs on amebocyte spontaneous aggregation and endotoxin induced aggregation were studied. N-Ethylmaleimide, ethylenediamine tetraacetate, propranolol, verapamil and lidocaine but not dibutyryl cyclic AMP could reduce the aggregating rate of amebocytes. The speed of degranulation induced by endotoxin was reduced by N-ethylmaleimide, ethylenediamine tetraacetate, propranolol, verapamil and caffeine. These results suggest that Ca+2 and c-AMP may play an important role in amebocyte spontaneous aggregation and endotoxin induced aggregation. The blocking agent of Ca+2 and c-AMP can also antagonize the aggregation of amebocytes.
Chen
I J
IJ
Department of Pharmacology, Kaohsiung Medical College, Taiwan, Republic of China.
Hwang
S J
SJ
Chen
Y M
YM
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D005260:Female; D008033:Limulus Test; D008297:Male; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D010975:Platelet Aggregation Inhibitors; D011433:Propranolol; D014700:Verapamil; D000242:Cyclic AMP; D002118:Calcium