An efficient technique for obtaining sequences flanking inserted retroviruses.
Genomic mapping studies frequently employ retrovirus-mediated transfer of dominant selectable markers to specific target chromosomes. DNA probes containing sequences adjacent to inserted proviruses are valuable mapping tools in such studies. We have implemented a strategy for amplification of chromosomal sequences flanking the 5' LTR of MoMuLV-based vectors. Probes derived from these amplification products successfully differentiated murine versus human proviral localization in retrovirus-infected mouse-human chromosome 17q hybrid cells.
Huckaby
C S
CS
Genmap Inc., New Haven, CT 06511.
Kouri
R E
RE
Lane
M J
MJ
Peshick
S M
SM
Carroll
W T
WT
Henderson
S M
SM
Faldasz
B D
BD
Waterbury
P G
PG
Vournakis
J N
JN
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D015342:DNA Probes; D004279:DNA, Viral / Q000302:isolation & purification; D008040:Genetic Linkage; D005822:Genetic Vectors; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes; D004279:DNA, Viral; D004247:DNA