Cell-specific expression of mRNAs encoding Na+,K(+)-ATPase alpha- and beta-subunit isoforms within the rat central nervous system.
We have used in situ hybridization histochemistry to analyze the subcellular distribution of mRNAs encoding Na,K-ATPase alpha- and beta-subunit isoforms in the rat central nervous system. Substantial differences in the cell-specific pattern of expression were found for the genes encoding three isoforms of the alpha subunit. Transcripts of alpha 1-subunit gene were detected in virtually all cell types and structures examined. Expression of alpha 2-subunit mRNA was characteristic of glia, whereas alpha 3-subunit transcripts were predominant in neurons. Transcripts encoding the beta 1 subunit were detected in neurons, whereas beta 2-subunit mRNA expression was characteristic of glia. mRNA encoding both beta-subunit isoforms was present in choroidal epithelial cells. The distribution pattern of alpha- and beta-subunit mRNAs in structures throughout the central nervous system is consistent with the possibility of six structurally distinct Na+,K(+)-ATPase isoenzymes.
Watts
A G
AG
Neural Systems Laboratory, Salk Institute, La Jolla, CA 92037.
Sanchez-Watts
G
G
Emanuel
J R
JR
Levenson
R
R
D000818:Animals; D001921:Brain / Q000201:enzymology; D002490:Central Nervous System / Q000166:cytology / Q000201:enzymology*; D002831:Choroid Plexus / Q000201:enzymology; D015870:Gene Expression; D007527:Isoenzymes / Q000235:genetics*; D046911:Macromolecular Substances; D008297:Male; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D013116:Spinal Cord / Q000201:enzymology; D014158:Transcription, Genetic
D007527:Isoenzymes; D046911:Macromolecular Substances; D015347:RNA Probes; D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase