A recombinant vaccinia virus expressing hepatitis A virus structural polypeptides: characterization and demonstration of protective immunogenicity.
A recombinant vaccinia virus containing most of the P1 region of hepatitis A virus (HAV) was constructed. Cell lysates of cultures infected with the virus contained HAV proteins detectable by radioimmunoassay. Western blot analysis revealed the presence of a single protein of Mr 60K to 62K, bearing epitopes from structural polypeptides VP4, -3 and -2, and the N terminus of VP1. The size of the protein suggests that at least some of the vaccinia virus thymidine kinase is also expressed. Inoculation of tamarin monkeys with the recombinant virus resulted in the development of a specific anti-HAV immune response which was protective against challenge with a virulent strain of HAV. Recombinant viruses expressing the above region of HAV or the proteins expressed by such viruses may be useful in the development of a vaccine suitable for use in man.
Karayiannis
P
P
Department of Medicine, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, U.K.
O'Rourke
S
S
McGarvey
M J
MJ
Luther
S
S
Waters
J
J
Goldin
R
R
Thomas
H C
HC
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D006506:Hepatitis A / Q000517:prevention & control*; D006507:Hepatovirus / Q000235:genetics / Q000276:immunology*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010957:Plasmids; D012452:Saguinus; D014614:Vaccines, Synthetic / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*; D014761:Viral Hepatitis Vaccines / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics* / Q000276:immunology
D004279:DNA, Viral; D014614:Vaccines, Synthetic; D014761:Viral Hepatitis Vaccines; D015678:Viral Structural Proteins