title: Iron sources for Haemophilus ducreyi.
abstract: The ability of various haem- and non-haem-iron-containing compounds to support the growth of iron-limited cultures of Haemophilus ducreyi was assessed in a plate bioassay. Only haemin or the haem-containing proteins, bovine haemoglobin, human haemoglobin and bovine catalase, but not equine cytochrome C111, were capable of serving as the sole exogenous iron source. Complexes of haptoglobin-haemoglobin and haem-serum albumin retained the ability to function as iron substrates. In contrast, no growth was observed with FeCl3, human lactoferrin and human transferrin. Siderophore production was not detected with a universal chemical assay. Outer-membrane-protein profiles derived from iron-starved cultures revealed four iron-regulated polypeptides of 65, 50, 45.5 and 40.5 Kda. These results indicate that haem can supply the requisite iron for growth of H. ducreyi.
authors.affiliation: Department of Microbiology, University of Calgary, Alberta, Canada.
authors.forename: B C
authors.initials: BC
authors.lastname: Lee
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D002374:Catalase / Q000378:metabolism; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006191:Haemophilus ducreyi / Q000254:growth & development / Q000378:metabolism*; D006427:Hemin / Q000378:metabolism; D006441:Hemoglobin A / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D011990:Receptors, Transferrin / Q000096:biosynthesis; D017262:Siderophores; D014168:Transferrin / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D007502:Iron Chelating Agents; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D017262:Siderophores; D014168:Transferrin; C038340:lactoferrin receptors; D006427:Hemin; D006441:Hemoglobin A; D007501:Iron; D002374:Catalase; D007781:Lactoferrin