Incorporation of a Calliphora arylphorin gene into the germ line of Drosophila by p-element-mediated transformation.
Two arylphorin gene clones were inserted into the transposable p-element vector Carnegie 20 and injected into Drosophila rosy- mutants. Transponed animals were selected by their red eye colour and remated. We demonstrate by hybridization experiments the stable integration of the injected genes in the germ line of Drosophila. However, the incorporated genes are not expressed.
Dorsch
M
M
Zoologisches Institut, Universität Würzburg, Bundesrepublik Deutschland.
Scheller
K
K
D000818:Animals; D004251:DNA Transposable Elements; D004175:Diptera / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000502:physiology; D006023:Glycoproteins / Q000235:genetics; D007301:Insect Hormones / Q000235:genetics*; D019476:Insect Proteins; D007814:Larva; D010957:Plasmids; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D006023:Glycoproteins; D007301:Insect Hormones; D019476:Insect Proteins; C041137:storage proteins, Insecta