Opioid receptor proteins in human brain. Detection with monoclonal anti-idiotypic antibodies.
In order to identify opioid receptor proteins, we first ascertained the presence of mu-receptors in membranes of the human cerebral frontal cortex, using binding studies performed with sufentanil. Sufentanil binding was reversed by naloxone and prevented by an anti-idiotypic opioid receptor-specific antibody. This antibody was bound specifically by proteins (molecular weights of 66 and 68 Kda) detected in frontal cortex membrane preparations by sodium dodecyl sulphate-polyacrylamide-gel electrophoresis in combination with western blot analysis. Binding of the same antibody was achieved in dot blots of homologous cerebral and cerebellar cortex preparations.
Traub
M
M
Endorphin Laboratories, Institute of Forensic Medicine, University of Zürich, Switzerland.
Spindler
K D
KD
Schmitt
H P
HP
Messiha
F S
FS
Bär
W
W
Pasi
A
A
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D008970:Molecular Weight; D011957:Receptors, Opioid / Q000032:analysis* / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D011957:Receptors, Opioid