Purification of two forms of arachidonate 15-lipoxygenase from human leukocytes.
Two different proteins with arachidonate 15-lipoxygenase activity have been purified to near homogeneity from human leukocytes. Both have the same molecular mass (74 kDa) on SDS/PAGE and appear to be equally active with three different fatty acid substrates. The N-terminal amino acid sequences of both forms were identical to the sequence of human reticulocyte 15-lipoxygenase [Sigal, E., Craik, C.S., Highland, E., Grunberger, D., Costello, L.L., Dixon, R.A.F. & Nadel, J.A. (1988) Biochem. Biophys. Res. Commun. 157, 457-464]. The two forms of 15-lipoxygenase could be clearly separated by cation-exchange chromatography. Of particular interest, the relative amounts of the two forms differed markedly between leukocytes obtained from normal donors and leukocytes from an individual with eosinophilia.
Izumi
T
T
Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
Rådmark
O
O
Jörnvall
H
H
Samuelsson
B
B
D000595:Amino Acid Sequence; D001093:Arachidonate 15-Lipoxygenase / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification; D016718:Arachidonic Acid / Q000378:metabolism; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017886:Durapatite; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D007527:Isoenzymes / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008042:Linolenic Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D012156:Reticulocytes / Q000201:enzymology; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D017965:gamma-Linolenic Acid
D006882:Hydroxyapatites; D007527:Isoenzymes; D008041:Linoleic Acids; D008042:Linolenic Acids; D016718:Arachidonic Acid; D017965:gamma-Linolenic Acid; D017886:Durapatite; D019787:Linoleic Acid; D001093:Arachidonate 15-Lipoxygenase