Simple methods for making EcoK and McrA restrictionless mutants.
Hiom
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Genetics Division, National Institute for Medical Research, Mill Hill, London, UK.
Sedgwick
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SG
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015253:Deoxyribonucleases, Type I Site-Specific / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D009154:Mutation; D013014:SOS Response, Genetics; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays
D004262:DNA Restriction Enzymes; C029602:endodeoxyribonuclease EcoK; C060629:endodeoxyribonuclease McrA; D015253:Deoxyribonucleases, Type I Site-Specific