Two pathways of signal transduction are activated in the same cell by different cytokines.
NFS60, a murine leukemia cell line, responds to both interleukin 3 and 6 by proliferating, apparently by different signal transduction pathways. Although stimulation by both cytokines increases the uptake of 3H-arachidonic acid, the response to IL-6 was much faster. Furthermore, the effect of various arachidonic acid metabolites on the response to cytokine was different. PGE2 inhibited IL-6-induced proliferation and potentiated the response to IL-3. Additionally the G proteins which coupled the IL-3 and IL-6 receptor to the proliferative response are probably different, based on the ability of cholera toxin to inhibit the IL-3 but not the IL-6 response. These data are evidence of two pathways of signal transduction.
Barton
B E
BE
Department of Allergy, Schering-Plough Research, Bloomfield, New Jersey 07003.
Mayer
R
R
Jackson
J V
JV
Clark
M A
MA
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000378:metabolism; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D016207:Cytokines; D007377:Interleukin-3; D015850:Interleukin-6; D011453:Prostaglandins; D010566:Virulence Factors, Bordetella; D016718:Arachidonic Acid; D000001:Calcimycin; D000242:Cyclic AMP; D011493:Protein Kinase C; D019204:GTP-Binding Proteins