title: The outer membrane permeability-increasing action of deacylpolymyxins.
abstract: The outer membrane permeability-increasing action of deacylpolymyxins was compared to the well-known potent action of polymyxin B nonapeptide (PMBN). Deacylpolymyxin B (DAPB), prepared by treating polymyxin B with polymyxin acylase, was found to be a slightly more effective permeabilizer than PMBN. As low a DAPB concentration as 1 microgram/ml sensitized Escherichia coli to the probe antibiotics (rifampin, fusidic acid, erythromycin, clindamycin, novobiocin) by factors 30-100 and Salmonella typhimurium by factors 10-100. A higher concentration (3 micrograms/ml) of DAPB elicited further sensitization. Also deacylcolistin (DAC) was found to be an effective permeabilizer.
authors.affiliation: Department of Fisheries, Faculty of Agriculture, Miyazaki University, Japan.
authors.forename: P
authors.initials: P
authors.lastname: Viljanen
authors.forename: H
authors.initials: H
authors.lastname: Matsunaga
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Vaara
mesh_terms: D000595:Amino Acid Sequence; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects; D008969:Molecular Sequence Data; D011112:Polymyxin B / Q000031:analogs & derivatives / Q000494:pharmacology; D011113:Polymyxins / Q000138:chemical synthesis / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects
chemical_list: D011113:Polymyxins; C069640:deacylpolymyxin B; C038934:polymyxin B nonapeptide; D011112:Polymyxin B