title: Phenotypic and genotypic analysis of Borrelia burgdorferi isolates from various sources.
abstract: A total of 17 B. burgdorferi isolates from various sources were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins, restriction enzyme analysis, Southern hybridization with probes complementary to unique regions of evolutionarily conserved genes (16S rRNA and fla), and direct sequencing of in vitro polymerase chain reaction-amplified fragments of the 16S rRNA gene. Three groups were distinguished on the basis of phenotypic and genotypic traits, the latter traced to the nucleotide sequence level.
authors.affiliation: Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, Federal Republic of Germany.
authors.forename: T
authors.initials: T
authors.lastname: Adam
authors.forename: G S
authors.initials: GS
authors.lastname: Gassmann
authors.forename: C
authors.initials: C
authors.lastname: Rasiah
authors.forename: U B
authors.initials: UB
authors.lastname: Göbel
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D001483:Base Sequence; D015139:Blotting, Southern; D015748:Borrelia burgdorferi Group / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004262:DNA Restriction Enzymes