Herpesvirus simiae (B virus) antibody response and virus shedding in experimental primary infection of cynomolgus monkeys.
Four cynomolgus monkeys (Macaca fascicularis) were inoculated in the lips and tongues with B virus. Virus shedding and antibody responses were monitored for up to 50 days postinfection. Virus was isolated from the oral cavities of all monkeys at 6 days postinfection despite the absence of observable lesions. Virus was not isolated from genital swabs or serum. Antibodies to both B virus and herpes simplex virus were detected by neutralization between days 8 and 12. Virus-specific IgM and IgG antibodies were measured by antibody capture radioimmunoassay. IgM was first detected on day 6; by contrast, IgG did not appear until day 12. Antibodies reactive in a competitive radioimmunoassay appeared by day 12 and peaked at 30 to 40 days postinfection. This study provides data on which to base the diagnosis of primary B virus infection in cynomolgus monkeys.
Lees
D N
DN
Virus Reference Laboratory, Central Public Health Laboratory, London, United Kingdom.
Baskerville
A
A
Cropper
L M
LM
Brown
D W
DW
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D005260:Female; D006566:Herpesviridae Infections / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D006568:Herpesvirus 1, Cercopithecine / Q000276:immunology* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008252:Macaca fascicularis / Q000382:microbiology*; D009500:Neutralization Tests; D011863:Radioimmunoassay
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M