Determination of estrogenic/antiestrogenic potential of antifertility substances using rat uterine peroxidase assay.
The effect of three compounds (clomiphene citrate, centchroman, embelin) and plant-derived methanolic extracts (Abutilon indicum and Butea monosperma) was studied on uterotropic and uterine peroxidase activities in ovariectomized rats. It was observed that these two parameters were highly correlated in response to treatment with these test materials and also to estradiol. It was suggested that the uterine peroxidase assay could be utilized as a biochemical parameter in the screening of new antifertility agents for their estrogenic/antiestrogenic properties.
Johri
R K
RK
Regional Research Laboratory (CSIR) Jammu Tawi, India.
Pahwa
G S
GS
Sharma
S C
SC
Zutshi
U
U
D000818:Animals; D016227:Benzoquinones / Q000494:pharmacology; D002486:Centchroman / Q000494:pharmacology; D002996:Clomiphene / Q000494:pharmacology; D003276:Contraceptives, Oral / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007124:Immunoenzyme Techniques; D009929:Organ Size / Q000187:drug effects; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014599:Uterus / Q000033:anatomy & histology
D016227:Benzoquinones; D003276:Contraceptives, Oral; D010936:Plant Extracts; D002996:Clomiphene; D002486:Centchroman; D004958:Estradiol; D009195:Peroxidase; C010945:embelin