Dominant suppression of adenovirus mediated transformation and insufficiency of p105Rb binding as a condition for oncogenic transformation.
An adenovirus-specific transformation resistant cell line (G2) expressing biologically active E1a proteins and originally isolated as a revertant from Ad2-transformed rat cells (F4), was shown to form stable Rb-E1a and 300K-E1a complexes in immunoprecipitation experiments. Consistent with the transformation resistant phenotype, cell hybrids between G2 and F4 were all nontumorigenic. Retrovirus insertion mutagenesis resulted in tumorigenic cell lines and identified a common locus responsible for the E1a-specific dominant tumor suppressor phenotype of G2 cells.
Weber
J M
JM
Département de Microbiologie, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.
Sircar
S
S
Rodrigues
M
M
Cai
F
F
Horvath
J
J
D000260:Adenoviruses, Human / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002472:Cell Transformation, Viral / Q000235:genetics*; D004251:DNA Transposable Elements / Q000502:physiology; D004279:DNA, Viral / Q000235:genetics*; D005347:Fibroblasts / Q000378:metabolism; D005799:Genes, Dominant / Q000235:genetics*; D016161:Genes, Retinoblastoma / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D006822:Hybrid Cells / Q000378:metabolism; D010641:Phenotype; D011233:Precipitin Tests; D051381:Rats; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism*; D013489:Suppression, Genetic / Q000235:genetics* / Q000502:physiology
D004251:DNA Transposable Elements; D004279:DNA, Viral; D016160:Retinoblastoma Protein