Nuclear localization of budgerigar fledgling disease virus capsid protein VP2 is conferred by residues 308-317.
The capsid protein VP2 of budgerigar fledgling disease virus (BFDV) contains two sequences (residues 309-315 and 334-340) which are homologous to the prototypic nuclear localization sequence (NLS) of the simian virus 40 T-antigen. Using recombinant potential NLS-beta-galactosidase fusion proteins we identified amino acid residues 308-317 (VPKRKRKLPT) to be the NLS of BFDV capsid proteins VP2 and VP3. Microfluorometry studies show that the BFDV-VP2 signal is considerably more efficient in nuclear transport kinetics, than the NLS of SV40-VP2, corresponding to amino acid residues 317-326 (PNKKKRKLSR).
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Institut für Mikro- und Molekularbiologie, Justus-Liebig-Universität Giessen, Germany.
Peters
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Hobom
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D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002213:Capsid / Q000737:chemistry* / Q000378:metabolism; D036022:Capsid Proteins; D002467:Cell Nucleus / Q000382:microbiology*; D007700:Kinetics; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013539:Simian virus 40 / Q000737:chemistry / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014709:Vero Cells
D036022:Capsid Proteins; D011994:Recombinant Proteins; C442543:VP2 protein, Polyomavirus