[ELISA for detection of anti-urease antibodies of Campylobacter pylori and its application].
The urease extracted from 6 strains of Helicobacter pylori (HP) was used as antigen to detect sera anti-urease antibodies of HP in 136 patients with gastric diseases and 13 persons with normal gastric mucosa by enzyme-linked immunosorbent assay (ELISA). The antigen proved to be pure and specific for ELISA. For a titre of 450EU or over 450EU the test had a sensitivity of 91.96% and a specificity of 86.49%. The results of the ELISA showed excellent correlation with microbiological detection of HP and histological examination of the antrum. The level of sera antibody showed a relation to the bacterial amount of HP in gastric mucosa, but no connection with the severity of gastritis. While ELISA may be effective for the serodiagnosis of HP infection and is able to distinguish patients with normal antrum mucosa from those with gastritis. The test does not help to distinguish those with antrum gastritis from those with peptic ulcer. It also provides a reliable method for epidemiological investigation of HP infection.
Chen
Z
Z
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis; D004381:Duodenal Ulcer / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology; D005756:Gastritis / Q000382:microbiology*; D016481:Helicobacter Infections; D016480:Helicobacter pylori / Q000201:enzymology / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014510:Urease / Q000276:immunology
D000907:Antibodies, Bacterial; D014510:Urease