Comparative studies on the antigenicity of extra- and intracellular viruses of fowl pox.
The intra- and extracellular virus of three strains of fowl pox virus, when precipitated in succession with different saturation of ammonium sulphate revealed three antigens in gel diffusion test in the precipitates obtained at 25%, 50% and 75% of saturation. Further analysis of each positive antigen by dot ELISA revealed that the extracellular virus of FS-8 and HP 1 strains possessed excess antigenic protein at 50% saturation compared to their intracellular viruses. While no difference between extra- and intracellular viruses of FS-4 strain was observed.
Maiti
N K
NK
Department of Veterinary Bacteriology and Virology, Punjab Agricultural University, Ludhiana, India.
Oberoi
M S
MS
Sharma
S N
SN
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D002642:Chick Embryo; D004797:Enzyme-Linked Immunosorbent Assay; D005586:Fowlpox / Q000276:immunology / Q000382:microbiology*; D005587:Fowlpox virus / Q000276:immunology*; D013045:Species Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral