title: [Isoelectric focusing of methylation and restriction enzymes from Staphylococcus aureus strain 6782].
abstract: The behaviour of methylation and restriction enzymes of Staphylococcus aureus 6782 during their isoelectrofocusing on ampholinese was studied. It was found that the RSau 6782 isoenzyme is represented by two isoforms, RI and RII, with isoelectric points of 4.2 and 7.9, respectively. Data from isoelectrofocusing analysis suggest that RI and R II are devoid of relaxed specificity found in the original preparation. In was shown that the relaxed specificity is also inherent in the isoschisomeric enzyme, RSau3A. Isoelectrofocusing of the original preparation RSau3A, as in case with RSau 6782, allows the identification of two peaks, RI and RII, and the separation of each peak from the "trace" activity. Multiple forms of DNA-methylase of the Sau 6782 type are represented by four isoenzymes possessing acidic properties. The method allows one to single out from the total methylase pool a modifying methylase with p1 (3.9) is close to that of RSau 6782 and thus the enzyme cannot serve for correct separation of restriction and methylation enzymes of Sau 6782.
authors.forename: E E
authors.initials: EE
authors.lastname: Arutiunian
authors.forename: N A
authors.initials: NA
authors.lastname: Gonchar
authors.forename: I Ia
authors.initials: IIa
authors.lastname: Levchenko
authors.forename: I M
authors.initials: IM
authors.lastname: Gruber
authors.forename: I I
authors.initials: II
authors.lastname: Nikol'skaia
mesh_terms: D002850:Chromatography, Gel; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008745:Methylation; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: C064811:DNA modification methylase MboI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D004262:DNA Restriction Enzymes
vernacular_title: Izoélektrofokusirovanie fermentov metilirovaniia i restrikstii shtamma Staphylococcus aureus 6782.