Functional endothelin-1 receptors in rat astrocytoma C6.
Rat astrocytoma C6 cells have been recently identified as target cells for ET-1, which stimulates inositol lipid turnover in these cells. It is shown here that binding of ET-1 to high-affinity receptors on C6 cells leads to 40-45% inhibition of isoproterenol-induced intracellular cyclic AMP accumulation, as well as to stimulation of inositol lipid turnover, both effects characterized by an absolute requirement of extracellular calcium. Moreover, ET-1, which has been generally reported to have a mitogenic effect on a variety of target cells including primary rat astrocytes, is shown here to stimulate or, alternatively, inhibit DNA synthesis in C6 cells, depending on the subclone considered.
Couraud
P O
PO
Laboratoire d'Immuno-Pharmacologie moléculaire, CNRS et Université Paris VII, Institut Cochin de Génétique Moléculaire (ICGM), France.
Durieu-Trautmann
O
O
Nguyen
D L
DL
Marin
P
P
Glibert
F
F
Strosberg
A D
AD
D000818:Animals; D001011:Aorta / Q000378:metabolism; D001254:Astrocytoma / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004273:DNA, Neoplasm / Q000096:biosynthesis; D016232:Endothelins / Q000138:chemical synthesis / Q000378:metabolism; D007295:Inositol Phosphates / Q000096:biosynthesis; D007545:Isoproterenol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007700:Kinetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017466:Receptors, Endothelin; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism*; D014757:Viper Venoms / Q000138:chemical synthesis / Q000378:metabolism
D004273:DNA, Neoplasm; D016232:Endothelins; D007295:Inositol Phosphates; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D014757:Viper Venoms; C057354:sarafotoxins s6; D005576:Colforsin; D000242:Cyclic AMP; D007545:Isoproterenol; D013936:Thymidine