Autoradiographic localisations of glutamatergic ligand binding sites in Xenopus brain.
In the Xenopus central nervous system the binding sites for [3H]kainate and [3H]alpha-amino-3-hydroxy-5-methylisoxazolepropionate [( 3H]AMPA) are highly localised and the distributions of both ligands are largely coincident. The telencephalon was the most strongly labelled area with a relatively uniform distribution of binding sites. In addition, the infundibulum and the cerebellum were also heavily labelled. Areas containing a lower density of binding sites included the septum, the thalamus and the optic lobes. [3H]Kainate binding was potently inhibited by 1 microM AMPA in the presence of 0.1 M KSCN and [3H]AMPA binding was blocked by 1 microM kainate. These data are consistent with the hypothesis that kainate and AMPA bind to the same site on a single protein entity and that this unitary AMPA/KA binding protein may constitute the predominant type of excitatory amino acid receptor in the Xenopus brain.
Henley
J M
JM
Department of Pharmacology, Medical School, University of Birmingham, U.K.
Bond
A
A
Barnard
E A
EA
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D001923:Brain Chemistry; D007051:Ibotenic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D007608:Kainic Acid / Q000378:metabolism; D016202:N-Methylaspartate / Q000494:pharmacology; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis; D017981:Receptors, Neurotransmitter / Q000032:analysis*; D014981:Xenopus; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D007051:Ibotenic Acid; D016202:N-Methylaspartate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D007608:Kainic Acid