title: Structure and expression of rat S-100 beta subunit gene.
abstract: The gene structure for S-100 beta subunit has been elucidated. The gene spans about 8 kbp and consists of 3 exons and 2 introns. The transcription initiation site was determined by an S1 nuclease mapping. The promoter region contains TATA-box-like and CAAT-box-like sequences. To examine the activity of the promoter sequence, a transfection of pS100 beta-lacZ fused gene to the cultured cells was carried out. C6 glioma cells showed a positive expression of beta-galactosidase. Gene-deletion experiments suggested the functional importance of the DNA fragment (22 bp) containing TATA-box-like and CAAT-box-like sequences. A factor protein that binds to the 100 bp DNA fragment containing the promoter sequence was specifically detected in the rat brain nuclear extract.
authors.affiliation: Department of Neuropharmacology, Niigata University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Maeda
authors.forename: H
authors.initials: H
authors.lastname: Usui
authors.forename: K
authors.initials: K
authors.lastname: Araki
authors.forename: R
authors.initials: R
authors.lastname: Kuwano
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology; D005091:Exons; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D006367:HeLa Cells / Q000502:physiology; D006801:Humans; D007438:Introns; D008099:Liver / Q000502:physiology; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D009418:S100 Proteins / Q000235:genetics*; D016385:TATA Box; D014158:Transcription, Genetic
chemical_list: D046911:Macromolecular Substances; D012333:RNA, Messenger; D009418:S100 Proteins