v-myb and v-ets cooperate for the mitogenic stimulation of primary fibroblasts by avian E26 retrovirus.
By using a series of deletion mutants, we have shown that the stimulation of fibroblast growth by E26 requires the cooperation of the two oncogenes, v-myb and v-ets, fused in the nuclear viral product. Of the two DNA-binding domains, only one must be present to promote anchorage-independent growth, whereas that of v-myb is required to allow growth in low serum medium. Furthermore, the v-ets oncogene comprises multifunctional domains.
Ravel-Chapuis
P
P
Laboratoire de Biologie Moléculaire et Cellulaire, UMR 13 CNRS, Institut National de la Recherche Agronomique, Ecole Normale Supérieure de Lyon, France.
Leprince
D
D
Pain
B
B
Li
R
R
Domenget
C
C
Stehelin
D
D
Samarut
J
J
Jurdic
P
P
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D002455:Cell Division; D002478:Cells, Cultured; D002642:Chick Embryo; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000502:physiology*; D066298:In Vitro Techniques; D009687:Nuclear Proteins / Q000502:physiology*; D020626:Oncogene Proteins v-myb; D015183:Restriction Mapping; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D013329:Structure-Activity Relationship
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D020626:Oncogene Proteins v-myb; D015682:Retroviridae Proteins, Oncogenic; C050379:oncogene proteins v-ets