[Rapid diagnosis of infection by cytomegalovirus using early-antigen detection (shell vial)].
We performed an evaluation of early cytomegalovirus antigen detection (Shell-vial method) for rapid diagnosis of cytomegalovirus infection in patients at risk. A comparative, parallel, prospective study against conventional cell culture in 913 clinical specimens was carried out. Shell-vial technique detected an early cytomegalovirus antigen in culture using an indirect immunofluorescent method with monoclonal antibodies. Cytomegalovirus was detected in 210 specimens (23.0%). In 24 instances (11.4%), cytomegalovirus was only detected by culture, in contrast with 54 specimens (25.7%) that were only positive in Shell-vial (p less than 0.001; McNemar test). Mean detection time for cell culture was 9.2 days, and 16-24 h for the Shell-vial technique. This is a rapid, sensitive, specific and simple to perform method for diagnosis of cytomegalovirus infection. Nevertheless, the predictivity of cytomegalovirus disease was not improved by this technique.
Pulido
M A
MA
Servicio de Microbiología, Hospital de Bellvitge-Prínceps d'Espanya, Universidad de Barcelona.
Escribano
E
E
López
P
P
Ayats
J
J
Pérez
J L
JL
Martín
R
R
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000302:isolation & purification* / Q000502:physiology; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000382:microbiology; D005347:Fibroblasts; D006801:Humans; D017874:Immediate-Early Proteins; D016867:Immunocompromised Host; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013997:Time Factors; D014776:Virus Cultivation
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus
Diagnóstico rápido de la infección por citomegalovirus mediante detección de antígeno temprano (shell-vial).