[A comparison of the results of analyzing the clinical data from gynecological, ophthalmological and dental patients using a set of rapid methods for the diagnosis of herpesvirus infection].
Analysis of biological specimens from gynecological, ophthalmological, and stomatological patients was carried out using current express methods for diagnosis of herpes virus infection developed by the authors: enzyme immunoassay (EIA), time-resolved fluoroimmunoassay (tr-FIA), molecular hybridization and conventional immunofluorescence technique. Monoclonal antibodies (MCA) generated by us with epitope specificity for gpD HSV-2 were used in EIA and tr-FIA. F(ab')2-fragments of MCA used as sorbents and for preparation of conjugates were found to be by 250% more sensitive than intact IgG used for similar purposes. The employment of EIA based on MCA F(ab')2-fragments contributed to detection of 0.2-0.6 ng of virus-specific protein in a specimen. Comparison of the results of analysis of clinical specimens of broad spectrum (mucous secreta from the cervical canal of the uterus and posterior vault of the vagina, saliva, lacrimal fluid) by the above express methods for diagnosis of herpes virus infection revealed a high percentage of positive findings, none of the methods providing 100% diagnosis. The use of a set of methods makes the resultant information more objective. Therefore, in future the data obtained by a number of current express methods for herpes diagnosis should be compared.
L'vov
N D
ND
Nikitina
A A
AA
Sveshnikov
P G
PG
Vanag
A I
AI
Viduta
O D
OD
Bresskiĭ
A G
AG
Marchenko
L A
LA
Sokolova
M V
MV
Vlasova
M A
MA
Blizniuk
V V
VV
D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000032:analysis; D002582:Cervix Mucus / Q000276:immunology; D005128:Eye Diseases / Q000276:immunology*; D005260:Female; D005831:Genital Diseases, Female / Q000276:immunology*; D006558:Herpes Genitalis / Q000175:diagnosis; D006561:Herpes Simplex / Q000175:diagnosis*; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D009059:Mouth Diseases / Q000276:immunology*; D012463:Saliva / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology; D013666:Tears / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral
Sopostavlenie rezul'tatov analiza klinicheskikh materialov ginekologicheskogo, oftal'mologicheskogo i stomatologicheskogo profileĭ naborom ékspress-metodov diagnostiki gerpesvirusnoĭ infektsii.