title: Glycosyl-phosphatidylinositol/inositol phosphoglycan: a signaling system for the low-affinity nerve growth factor receptor.
abstract: Nerve growth factor (NGF) exerts a variety of actions during embryonic development. At the early stages of inner ear development, NGF stimulates cell proliferation, an effect mediated through low-affinity receptors. We have studied the possibility that the glycosyl-phosphatidylinositol/inositol phosphoglycan (glycosyl-PtdIns/IPG) system is involved in transmitting this NGF signal. Endogenous glycosyl-PtdIns was characterized in extracts of cochleovestibular ganglia (CVGs) that incorporated [3H]glucosamine, [3H]galactose, [3H]myristic acid, and [3H]palmitic acid. Incubation of CVG with NGF produced a rapid and transient hydrolysis of glycosyl-PtdIns. Hydrolysis was complete at 100 ng/ml, and the half-maximal effect occurred at 25 ng/ml, overlapping with the concentration dependence of the mitogenic effect of NGF. An IPG was isolated from embryonic extracts. It had biological effects similar to those reported for the insulin-induced IPG in other tissues. It exerted a powerful mitogenic effect on CVG, comparable to that of NGF. Both the IPG- and NGF-induced cell proliferation were blocked by anti-IPG antibodies that recognized the endogenous IPG on a silica plate immunoassay. These results show that CVG possesses a fully active glycosyl-PtdIns/IPG signal transduction system and that the proliferative effects associated with NGF binding to low-affinity receptors require IPG generation.
authors.affiliation: Departamento Ciencias Morfológicas, Facultad de Medicina, Universidad de Valladolid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Represa
authors.forename: M A
authors.initials: MA
authors.lastname: Avila
authors.forename: C
authors.initials: C
authors.lastname: Miner
authors.forename: F
authors.initials: F
authors.lastname: Giraldez
authors.forename: G
authors.initials: G
authors.lastname: Romero
authors.forename: R
authors.initials: R
authors.lastname: Clemente
authors.forename: J M
authors.initials: JM
authors.lastname: Mato
authors.forename: I
authors.initials: I
authors.lastname: Varela-Nieto
mesh_terms: D000818:Animals; D002455:Cell Division; D002642:Chick Embryo; D007758:Ear, Inner / Q000254:growth & development; D006017:Glycolipids / Q000502:physiology*; D017261:Glycosylphosphatidylinositols; D006868:Hydrolysis; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000502:physiology*; D010716:Phosphatidylinositols / Q000502:physiology*; D011134:Polysaccharides / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000502:physiology*; D017475:Receptors, Nerve Growth Factor; D015398:Signal Transduction
chemical_list: D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; C028039:inositol phosphate glycan