Regional distribution of lipoxygenase activities in porcine ciliary epithelium.
Nonpigmented epithelial (NPE) cells and pigmented epithelial (PE) cells were separated from porcine ciliary processes without protease treatment. The relative capabilities of NPE cells and PE cells to synthesize 5-hydroxyeicosatetraenoic acid (5-HETE), 12-HETE, and 5,12-diHETE from 1-[14C] arachidonic acid were determined. HETE compounds were separated by HPLC. The capability of NPE cells to synthesize 5-lipoxygenase and 12-lipoxygenase products was, respectively, five-fold and 2.5 fold higher than that of PE cells. The formation of 5-HETE, 12-HETE and 5,12-diHETE was inhibited virtually completely by 50 microM nordihydroguaiaretic acid but not by 5 microM indomethacin. The major product formed both by NPE cells and PE cells was 12-HETE.
Sakamoto
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S
Kresge Eye Institute, Department of Ophthalmology, Wayne State University School of Medicine, Detroit, Michigan.
Shichi
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H
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism*; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D016718:Arachidonic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002924:Ciliary Body / Q000201:enzymology*; D004848:Epithelium / Q000201:enzymology; D006893:Hydroxyeicosatetraenoic Acids / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D007213:Indomethacin / Q000494:pharmacology; D007975:Leukotriene B4 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D009637:Masoprocol / Q000494:pharmacology; D010857:Pigment Epithelium of Eye / Q000201:enzymology*; D013552:Swine
D000894:Anti-Inflammatory Agents, Non-Steroidal; D006893:Hydroxyeicosatetraenoic Acids; D016859:Lipoxygenase Inhibitors; D007975:Leukotriene B4; D016718:Arachidonic Acid; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D009637:Masoprocol; D001092:Arachidonate 12-Lipoxygenase; D001094:Arachidonate 5-Lipoxygenase; D007213:Indomethacin