Developmental expression of G proteins that differentially modulate adenylyl cyclase activity in mouse brain.
Changes in the relative abundance of the G protein alpha subunits were observed during early mouse development Gs alpha was almost exclusively present as a large form (Gs-1) in prenatal brain. Postnatally with a substantial increase in Gpp[NH]p stimulated adenylyl cyclase activity, the small form (Gs.s) increased in amount while Gs-1 decreased. These results suggest that the Gs-s may be the more effective cyclase activator and that changes in alternative splicing are developmentally regulated. Gi1 and Go appeared before birth whereas Gi2 developed postnatally. Opiate stimulation of GTPase and inhibition of adenylyl cyclase were fully expressed prenatally.
Rius
R A
RA
Section on Cellular Neurobiology, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Streaty
R A
RA
Peng Loh
Y
Y
Klee
W A
WA
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D000242:Cyclic AMP / Q000032:analysis; D005048:Etorphine / Q000494:pharmacology; D005260:Female; D020558:GTP Phosphohydrolases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D007700:Kinetics; D046911:Macromolecular Substances; D008297:Male; D008566:Membranes / Q000378:metabolism; D051379:Mice; D013574:Synaptosomes / Q000378:metabolism
D046911:Macromolecular Substances; D006165:Guanylyl Imidodiphosphate; D005048:Etorphine; D000242:Cyclic AMP; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases