Inactivation of human fibroblast collagenase by chloroacetyl N-hydroxypeptide derivatives.
When human fibroblast collagenase was incubated with ClCH2CO-(N-OH)Leu-Ala-Gly-NH2 (2-5 mM) in Tris buffer, pH 7.4 at 25 degrees C, a slow, time-dependent inhibition of the enzyme was observed. Dialysis against a buffer to remove free inhibitor did not reactivate the enzyme. A reversible competitive inhibitor, phthaloyl-GlyP-Ile-Trp-NHBzl (50 microM) partially protected the enzyme from inactivation by the compound. From the concentration dependent rates of inactivation Ki = 0.5 +/- 0.1 mM and k3, the rate constant for inactivation = 3.4 +/- 0.3 x 10(-3) min-1 were determined. The inactivation followed the pH optimum (6.5-7.0) for the enzyme activity, suggesting direct involvement of the same active site residue(s). The reaction mode of the inhibitor may be analogous to that of the inactivation of Pseudomonas aeruginosa elastase [Nishino, N. and Powers, J. (1980) J. Biol. Chem., 255, 3482] in which the catalytic glutamate carboxyl was alkylated by the inhibitor after its binding to enzyme through the hydroxamic Zn2+ ligand. All carboxyl groups in the inactivated collagenase were modified with 0.1 M ethyl dimethylaminopropyl carbodiimide/0.5 M glycinamide in 4 M guanidine at pH 5. The inactivator-affected carboxyl group was then regenerated with 1 M imidazole at pH 8.9, 37 degrees C for 12 h and the protein was radiolabeled with 3H-glycine methyl ester and carbodiimide to incorporate 0.9 residue glycine per mol enzyme.
Lin
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Department of Enzymology, Merck Sharp & Dohme Research Laboratories, Rahway, NJ 07065.
Kuo
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D000595:Amino Acid Sequence; D017364:Collagenases; D004792:Enzyme Precursors / Q000037:antagonists & inhibitors*; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D061965:Matrix Metalloproteinase Inhibitors; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors; D013329:Structure-Activity Relationship
D004792:Enzyme Precursors; D061965:Matrix Metalloproteinase Inhibitors; D009842:Oligopeptides; D011994:Recombinant Proteins; D017364:Collagenases; C022440:procollagenase