Interaction of xenobiotics on the glucose-transport system and the Na+/K(+)-ATPase of human skin fibroblasts.
The effects of individual and combined xenobiotics on functional properties of the plasma membrane of human skin fibroblasts were investigated. Good correlations between toxic effects on the D-glucose transport system or the Na+/K(+)-ATPase and the lipophilicity of the substances could be observed. The linear regression coefficients plotting log EC20 values (doses, leading to 20% inhibition) versus log Pow (octanol/water partition coefficient) were r = 0.95 (P less than 0.05). The combination of lipophilic with less lipophilic xenobiotics, such as pentachlorophenol with 4-chloroaniline, leads to additional effects. However, when the detergent sodium dodecyl benzenesulfonate was combined with the herbicide 2,4-dichlorophenoxyacetate (2,4-D), the toxic effect of 2,4-D on the Na+/K(+)-ATPase decreased considerably. The results support in general the assumption that the inhibition of integral functional proteins is based on an accumulation of xenobiotics in the plasma membrane, probably due to the enhanced membrane fluidity. Thus, the basic toxicity of xenobiotics can be predicted by their physicochemical properties.
Cascorbi
I
I
Freie Universität Berlin, Institut für Biochemie und Molekularbiologie, Federal Republic of Germany.
Forêt
M
M
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000814:Aniline Compounds / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002462:Cell Membrane / Q000201:enzymology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002733:Chlorophenols / Q000494:pharmacology; D003847:Deoxyglucose; D004357:Drug Synergism; D005347:Fibroblasts / Q000201:enzymology; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects; D010416:Pentachlorophenol / Q000494:pharmacology; D012867:Skin / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D015262:Xenobiotics / Q000737:chemistry / Q000494:pharmacology*
D000814:Aniline Compounds; D002733:Chlorophenols; D015262:Xenobiotics; D015084:2,4-Dichlorophenoxyacetic Acid; D003847:Deoxyglucose; D010416:Pentachlorophenol; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose; C024564:2,4,6-trichlorophenol; C004658:4-chloroaniline