[Effects of hypoxia on phosphoinositide turnover and adenylate cyclase system in cultured endothelial cells].
By studying the effects of oxygen deficiency upon signal-transducing system it has been shown that in long hypobaric hypoxia activates PI-turnover in cultured human endothelial cells. The sensitivity of cells to histamine was decreased as well as the adenylate cyclase activity in membranes of this cells. The amount of beta-adrenoreceptors was not influenced significantly. Incubation of endothelial cells with histamine (10(-5) M) and phorbol ester (10(-9) M) = activator of protein kinase C within 1-2 h resulted in desensitization of cellular responses which can be seen not only as a disappearance of histamine-induced activation of PI-turnover but also as a decrease of beta-adrenoreceptor amount and adenylate cyclase activity. It seems that hypoxia may change the action of Ca-mobilizing hormones on PI-turnover and suppress adenylate cyclase in human endothelial cells. However the effects of hypoxia on signal-transducing systems in this cells are developed slower than those of Ca-mobilizing hormones.
Buravkova
L B
LB
Mirzapoiazova
T Iu
TIu
Grigorian
G Iu
GIu
Tkachuk
V A
VA
D000262:Adenylyl Cyclases / Q000378:metabolism*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D010703:Phorbol Esters / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000032:analysis; D014471:Umbilical Veins
D010703:Phorbol Esters; D010716:Phosphatidylinositols; D011943:Receptors, Adrenergic, beta; D006632:Histamine; D000262:Adenylyl Cyclases
Effekty gipoksii na fosfoinozitidnyĭ obmen i adenilattsiklaznuiu sistemu v kul'tiviruemykh éndotelial'nykh kletkakh.