title: Cloning of human heterohybridoma cell lines using chronic lymphocytic leukemia B cells as a feeder layer.
abstract: B cells from the peripheral blood of chronic lymphocytic leukemia patients were isolated by gradient density centrifugation and used without irradiation as a feeder layer in the cloning of human heterohybridoma cell lines by limiting dilution. Cloning efficiencies were high with all the cell lines tested. These feeder leukemia B cells could also be successfully used after having been stored in liquid nitrogen.
authors.affiliation: Servicio de Immunología, Hospital Universitario Virgen del Rocío, Sevilla, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Sanchez
authors.forename: M M
authors.initials: MM
authors.lastname: Robledo
authors.forename: J
authors.initials: J
authors.lastname: Yelamos
authors.forename: M F
authors.initials: MF
authors.lastname: Gonzalez
authors.forename: R
authors.initials: R
authors.lastname: Magariño
authors.forename: M P
authors.initials: MP
authors.lastname: Bermudo
authors.forename: A
authors.initials: A
authors.lastname: Nuñez-Roldan
mesh_terms: D000818:Animals; D002472:Cell Transformation, Viral; D002999:Clone Cells; D004854:Herpesvirus 4, Human; D006801:Humans; D006822:Hybrid Cells; D006825:Hybridomas / Q000166:cytology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology; D051379:Mice; D014407:Tumor Cells, Cultured