title: Development and testing of a packaging cell line for avian retroviral vectors.
abstract: A new helper cell line designated L3.07, has been used to package spleen necrosis virus (SNV)-based vectors, resulting in the production of high titres of replication defective retroviruses. One of these vectors, vSNO21 has been shown to infect avian primordial germ cells (PGCs).
authors.affiliation: AFRC Institute for Animal Health, Houghton Laboratory, Huntingdon, England.
authors.forename: N L
authors.initials: NL
authors.lastname: Meyers
authors.forename: S C
authors.initials: SC
authors.lastname: Booth
authors.forename: N
authors.initials: N
authors.lastname: Bumstead
authors.forename: L
authors.initials: L
authors.lastname: Vick
authors.forename: K
authors.initials: K
authors.lastname: Simkiss
mesh_terms: D000818:Animals; D002460:Cell Line; D003370:Coturnix; D005347:Fibroblasts; D005822:Genetic Vectors; D051379:Mice; D010957:Plasmids; D015183:Restriction Mapping; D013539:Simian virus 40 / Q000235:genetics* / Q000648:ultrastructure; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication
chemical_list: D015678:Viral Structural Proteins