Cell polarity regulates the release of secretory component, the epithelial receptor for polymeric immunoglobulins, from the surface of HT-29 colon carcinoma cells.
The HT-29 human colon carcinoma cell line differentiates in glucose-free medium to an enterocytic phenotype. We previously isolated a series of HT-29 subclones selected for high levels of expression of secretory component (SC), the epithelial receptor for polymeric immunoglobulins. To develop a model system for studying effects of cell polarity on SC expression and release from the cell surface, the HT-29.74 subclone was induced to differentiate in glucose-free medium. Expression of SC was induced by glucose deprivation in both the parental HT-29 cell line and, to an even greater extent, in the HT-29.74 subclone. Prolonged glucose deprivation of HT-29.74 cells resulted in morphological changes consistent with enterocytic differentiation. Metabolic radiolabeling of SC in differentiated HT-29.74 cells indicated that proteolytic cleavage of membrane-bound to free SC occurred both on the cell surface and intracellularly, possibly in a vacuolar apical compartment or intrapeithelial lumen. To study effects of cell polarity on SC release, differentiated HT-29.74 cells were depolarized by culturing in low calcium medium. Within 2 hours after transfer of the cells into low calcium medium, a burst of SC release was observed concomitant with cell depolarization. Subsequently, release of SC declined significantly and remained low as long as cells were maintained in a depolarized state. The extent of cell depolarization could be controlled by varying the extracellular calcium concentration or by substituting the divalent cation Sr++, which partially prevents depolarization, for Ca++. In either case, the magnitude of the initial burst and subsequent decline in release of SC was proportional to the extent of cell depolarization. We conclude that cell polarity plays an important role in controlling the release of SC in intestinal epithelial cells, most likely by regulating the distribution of membrane-bound SC and SC protease, which are on the basolateral and apical cell surfaces, respectively, in differentiated cells.
Chintalacharuvu
K R
KR
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
Piskurich
J F
JF
Lamm
M E
ME
Kaetzel
C S
CS
D002118:Calcium / Q000494:pharmacology; D002452:Cell Count; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D004848:Epithelium / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D008854:Microscopy, Electron; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012635:Secretory Component / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure
D007136:Immunoglobulins; D011956:Receptors, Cell Surface; D012635:Secretory Component; D005947:Glucose; D002118:Calcium