Identification of feline immunodeficiency virus rev gene activity.
We constructed 16 deletion mutants from an infectious molecular clone of feline immunodeficiency virus (FIV) and a reporter plasmid carrying the bacterial chloramphenicol acetyltransferase (CAT) gene to identify the rev transactivator activity of the virus. Cotransfections of various mutants and the rev reporter clone bearing a portion of FIV env in addition to the CAT gene revealed that the sequence important for the augmentation of CAT production was located in three separate parts of the virus genome. This enhancement was FIV specific in that the human retrovirus rev and rex gene products did not activate the reporter. The phenotypic properties of an FIV proviral mutant containing a small deletion in the genome were similar to those of rev mutants derived from primate immunodeficiency viruses. These results indicate that FIV, like the other lentiviruses, contains the rev gene in its genome.
Kiyomasu
T
T
Department of Viral Oncology, Kyoto University, Japan.
Miyazawa
T
T
Furuya
T
T
Shibata
R
R
Sakai
H
H
Sakuragi
J
J
Fukasawa
M
M
Maki
N
N
Hasegawa
A
A
Mikami
T
T
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002415:Cats; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral; D015757:Genes, rev; D016089:Immunodeficiency Virus, Feline / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016366:Open Reading Frames; D010957:Plasmids; D012367:RNA, Viral / Q000032:analysis / Q000737:chemistry; D015533:Transcriptional Activation; D014162:Transfection; D014779:Virus Replication
D012367:RNA, Viral; D015500:Chloramphenicol O-Acetyltransferase