Dot immunobinding assay for the detection of bluetongue virus antibodies in sheep experimentally inoculated with bluetongue virus type 1.
Dot immunobinding assay (DIA) was evaluated for the detection of bluetongue virus (BTV) antibodies in sheep experimentally inoculated with BTV 1. Serum samples collected on 14, 21, 28, 43 and 60 day post infection (dpi) were positive for precipitating antibodies by the agar gel precipitation test (AGPT) while antibodies could be detected as early as 7 dpi by DIA and ELISA. Virus neutralizing antibodies were detected first at 14 dpi. The sensitivity of the four tests was compared on the same serum samples collected at different intervals. The results indicated that DIA was more sensitive than AGPT and the serum neutralization test and as sensitive as ELISA. Thus due to sensitivity simplicity and economy, DIA could replace AGPT for diagnosis and serological survey for BTV infection in animals.
Chander
S
S
Department of Veterinary Microbiology, College of Veterinary Sciences, Haryana Agricultural University, Hisar, India.
Prasad
G
G
Jain
N C
NC
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001819:Bluetongue / Q000175:diagnosis / Q000276:immunology*; D001820:Bluetongue virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D009500:Neutralization Tests; D011233:Precipitin Tests; D011237:Predictive Value of Tests; D012756:Sheep
D000914:Antibodies, Viral