Bovine lactoferrin receptors in Staphylococcus aureus isolated from bovine mastitis.
A total of 103 Staphylococcus aureus strains isolated from bovine mastitis were tested for bovine lactoferrin binding in a 125I-labeled protein binding assay. More than 85% of the strains demonstrated high to moderate and a few showed little or no binding. Bovine lactoferrin binding to S. aureus cells was high when grown on blood, nutrient, or proteose-peptone agar, but the binding capacity was low with cells grown on salt rich media, in skim milk, or in broth. The kinetics of 125I-labeled bovine lactoferrin binding required approximately 90 min for complete saturation with optimal interaction in the pH range 4.0 to 7.0. The lactoferrin-staphylococci interaction was specific with a high affinity (association constant, Ka 14 x 10(6) L/mol). Scatchard plot analysis estimated the number of binding sites per cell at 7200 on strain SA-340. Unlabeled bovine lactoferrin effectively displaced the binding of the labeled ligand to strain SA-340 in a dose-dependent manner. Bovine lactoferrin binding was inhibited or displaced by human lactoferrin. Various plasma, connective tissue, or mucosal secretory proteins tested did not inhibit lactoferrin-staphylococci interaction. Bovine lactoferrin binding components on SA-340 were resistant to glycolytic enzymes and moderately susceptible to proteolytic digestion. Two proteins with an estimated molecular weight of approximately 92 and 67 kDa were identified as bovine lactoferrin binding components of S. aureus strain SA-340.
Naidu
A S
AS
Department of Medical Microbiology, University of Lund, Malmö General Hospital, Sweden.
Andersson
M
M
Miedzobrodzki
J
J
Forsgren
A
A
Watts
J L
JL
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D003470:Culture Media; D007781:Lactoferrin / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000382:microbiology*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000378:metabolism*
D003470:Culture Media; D011956:Receptors, Cell Surface; C038340:lactoferrin receptors; D007781:Lactoferrin