A one-minute oxidase test to detect Vibrio strains isolated from cultures on thiosulphate-citrate-bile salts-sucrose (TCBS) medium.
Vibrio cholerae is oxidase positive, a primary characteristic used to differentiate it from Enterobacteriaceae. But false negative oxidase test results have been obtained with colonies from thiosulphate-citrate-bile salts-sucrose (TCBS) agar medium. A rapid oxidase test procedure is described here. This takes 1 min, avoids false negative results and the necessity to grow the bacteria in a general-purpose medium. The bacteria may be recovered after the test and used for further investigations.
Vila
J
J
Servei de Microbiologia i Parasitologia, Hospital Clinic i Provincial de Barcelona, Facultat de Medicina, Universitat de Barcelona, Spain.
Abdalla
S
S
Gonzalez
J
J
Garcia
C
C
Bombi
J A
JA
Jimenez de Anta
M T
MT
D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D010088:Oxidoreductases / Q000032:analysis*; D014733:Vibrio / Q000201:enzymology / Q000302:isolation & purification* / Q000648:ultrastructure; D014734:Vibrio cholerae / Q000201:enzymology / Q000302:isolation & purification* / Q000648:ultrastructure
D003470:Culture Media; D010088:Oxidoreductases