Synthetic DNA bending sequences increase the rate of in vitro transcription initiation at the Escherichia coli lac promoter.
Appropriately phased DNA bending sequences replacing the CAP binding site upstream from the lac promoter increase by roughly tenfold the rate of specific transcription initiation from a superhelical promoter template in vitro; promoter occlusion results from polymerase binding to the upstream (dA)n.(dT)n tracts, but this phenomenon is not responsible for the observed phase-dependent transcriptional activity. The rates of open complex formation at both P1 and P2 promoters respond in a similar phase-dependent way to the synthetic curved DNA sequences.
Gartenberg
M R
MR
Department of Chemistry, Yale University, New Haven, CT 06511.
Crothers
D M
DM
D001483:Base Sequence; D001665:Binding Sites; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D004278:DNA, Superhelical / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D007785:Lactose / Q000378:metabolism*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic
D002373:Cyclic AMP Receptor Protein; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D012321:DNA-Directed RNA Polymerases; D007785:Lactose