title: Spin trapping by use of N-tert-butylhydroxylamine. Involvement of Fenton reactions.
abstract: Spin trapping of short-lived R. radicals is done by use of N-tert-butylhydroxylamine (1) and H2O2. The hydroxylamine is oxidized to the radical t-BuN(O)H (2) which is converted into the spin trap 2-methyl-2-nitrosopropane (3). Simultaneously, hydroxyl radicals .OH are formed from H2O/. The latter radical species abstracts hydrogen atoms from suitable molecules HR to give R. radicals, which are trapped with the formation of aminooxyl radicals, i.e., t-BuN(O)R (4), detectable by EPR spectroscopy. The reaction is enhanced by the presence of iron ions. The cleavage of H2O2 into .OH radicals is considered to involve both a radical-driven (t-BuN(O)H 2) and an iron-driven Fenton reaction.
authors.affiliation: Department of Medical Physics, University of Göteborg, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Lagercrantz
mesh_terms: D001555:Benzene Derivatives; D002384:Catalysis; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000737:chemistry; D006898:Hydroxylamines / Q000737:chemistry*; D007501:Iron / Q000494:pharmacology; D008956:Models, Chemical; D010084:Oxidation-Reduction; D004369:Pentetic Acid / Q000737:chemistry; D010545:Peroxides / Q000737:chemistry; D012997:Solvents; D013113:Spin Labels; D020122:tert-Butylhydroperoxide
chemical_list: D001555:Benzene Derivatives; D005609:Free Radicals; D006898:Hydroxylamines; D010545:Peroxides; D012997:Solvents; D013113:Spin Labels; C072771:N-tert-butylhydroxylamine; D004369:Pentetic Acid; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D007501:Iron; C007164:cumene hydroperoxide