A monoclonal ELISA for bovine viral diarrhoea pestivirus antigen detection in persistently infected cattle.
Detection of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is crucial to controlling mucosal disease. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies raised against the 48-kDa glycoprotein and the 120/80-kDa protein was developed for detecting antigens in leucocytes of 3 persistently BVDV-infected calves. The test is simple, sensitive and rapid. Moreover the same ELISA was able to recognise Belgian field isolates of BVDV. These results show that the test can be applied in the field.
Mignon
B
B
Department of Virology-Immunology, Faculty of Veterinary Medicine, Institut de Chimie, Sart Tilman-Liège, Belgium.
Dubuisson
J
J
Baranowski
E
E
Koromyslov
I
I
Ernst
E
E
Boulanger
D
D
Waxweiler
S
S
Pastoret
P P
PP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000097:blood*; D001667:Binding, Competitive; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis; D002417:Cattle; D002478:Cells, Cultured; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral