Isolation of Drosophila genes encoding G protein-coupled receptor kinases.
G protein-coupled receptors are regulated via phosphorylation by a variety of protein kinases. Recently, termination of the active state of two such receptors, the beta-adrenergic receptor and rhodopsin, has been shown to be mediated by agonist- or light-dependent phosphorylation of the receptor by members of a family of protein-serine/threonine kinases (here referred to as G protein-coupled receptor kinases). We now report the isolation of a family of genes encoding a set of Drosophila protein kinases that appear to code for G protein-coupled receptor kinases. These proteins share a high degree of sequence homology with the bovine beta-adrenergic receptor kinase. The presence of a conserved family of G protein-coupled receptor kinases in vertebrates and invertebrates points to the central role of these kinases in signal transduction cascades.
Cassill
J A
JA
Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093.
Whitney
M
M
Joazeiro
C A
CA
Becker
A
A
Zuker
C S
CS
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D017868:Cyclic AMP-Dependent Protein Kinases; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D004330:Drosophila / Q000201:enzymology / Q000235:genetics*; D029721:Drosophila Proteins; D005136:Eye Proteins; D051606:G-Protein-Coupled Receptor Kinase 1; D054769:G-Protein-Coupled Receptor Kinase 2; D019204:GTP-Binding Proteins / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods; D011494:Protein Kinases / Q000032:analysis / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D051552:beta-Adrenergic Receptor Kinases
D015342:DNA Probes; D029721:Drosophila Proteins; D005136:Eye Proteins; D011956:Receptors, Cell Surface; D004247:DNA; D011494:Protein Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D051606:G-Protein-Coupled Receptor Kinase 1; D051552:beta-Adrenergic Receptor Kinases; D054769:G-Protein-Coupled Receptor Kinase 2; C496574:Gprk2 protein, Drosophila; D019204:GTP-Binding Proteins