title: Expression of leukocyte adhesion molecules by mucosal mononuclear phagocytes in inflammatory bowel disease.
abstract: Leukocyte adhesion molecules are important in cell-cell interactions of the immune system. Lymphocyte function-associated antigen 1 (cluster designation 11a) mediates interactions between T cells and mononuclear phagocytes through its ligand, the intercellular adhesion molecule 1 (CD54), whereas complement receptors 3 (CD 11b) and 4 (CD11c) are involved in complement-mediated phagocytosis. Expression of CD11 molecules and intercellular adhesion molecule 1 was studied in colonic biopsy specimens from 20 patients with inflammatory bowel disease and 10 normal controls. In normal colon, few mononuclear phagocytes expressed lymphocyte function-associated antigen 1 and intercellular adhesion molecule 1 at high densities. The major adhesion molecule was CD11c. Thus, the largest population of normal colonic mononuclear phagocytes was represented by quiescent, resident macrophages with likely phagocytic function. In inflammatory bowel disease, mononuclear phagocytes showed only a slight increase in CD11a expression and no significant change in expression of CD11b and CD11c. By contrast, the percentage of mononuclear phagocytes expressing intercellular adhesion molecule 1 was increased from 6.9% +/- 3.9% in controls to 69.2% +/- 12.8% in ulcerative colitis (P less than 0.001) and to 45.7% +/- 22.8% in Crohn's disease (P less than 0.01), showing a close relationship with histological activity. The increased expression of intercellular adhesion molecule 1 in inflammatory bowel disease indicates a state of immunological activation induced by local release of inflammatory cytokines. Such induction of intercellular adhesion molecule 1 on mononuclear phagocytes may be important in the maintenance of chronic inflammation by facilitating interactions with T cells and T-cell antigen recognition.
authors.affiliation: Divisione di Medicina, Ospedale V. Cervello, Palermo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Malizia
authors.forename: A
authors.initials: A
authors.lastname: Calabrese
authors.forename: M
authors.initials: M
authors.lastname: Cottone
authors.forename: M
authors.initials: M
authors.lastname: Raimondo
authors.forename: L K
authors.initials: LK
authors.lastname: Trejdosiewicz
authors.forename: C J
authors.initials: CJ
authors.lastname: Smart
authors.forename: L
authors.initials: L
authors.lastname: Oliva
authors.forename: L
authors.initials: L
authors.lastname: Pagliaro
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis; D000943:Antigens, Differentiation / Q000096:biosynthesis; D018845:CD11 Antigens; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D003093:Colitis, Ulcerative / Q000276:immunology*; D003424:Crohn Disease / Q000276:immunology*; D005260:Female; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D018845:CD11 Antigens; D015815:Cell Adhesion Molecules; D006684:HLA-DR Antigens