Identification and characterization of viral polypeptides from type-II avian adenoviruses.
The polypeptides of serologically related viruses of hemorrhagic enteritis (HE) in turkeys, marble spleen disease (MSD) in pheasants, and splenomegaly in chickens (SMC) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed by protein immunoblotting with polyclonal antibodies to HE virus (HEV). The viral polypeptides II, III, IV, V, VI, and VII were detected on SDS-PAGE with the size range from 18 to 97 kDa in HEV. Viral polypeptides II, III, V, VI, and VII were detected in MSD virus and virus of SMC. Protein immunoblotting of viral proteins with anti-HEV serum revealed antigenic differences between the 3 viruses of avian adenovirus type-II examined. The differences were that the polypeptides II, III, IV, V, VI, and VII were identified in HEV and the polypeptides II, V, VI, and VII were identified in MSD virus and virus of SMC. The bands of penton base (polypeptide III) and fiber (polypeptide IV) were seen in HEV only by protein immunoblotting.
Zhang
C L
CL
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St Paul 55108.
Nagaraja
K V
KV
Sivanandan
V
V
Newman
J A
JA
D000257:Adenoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D000259:Aviadenovirus / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D001715:Bird Diseases / Q000382:microbiology*; D001717:Birds; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D008854:Microscopy, Electron; D010455:Peptides / Q000032:analysis / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D014422:Turkeys; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology; D014771:Virion / Q000648:ultrastructure
D007106:Immune Sera; D010455:Peptides; D014764:Viral Proteins