Steroidogenic enzyme activity in the rat testis following Leydig cell destruction by ethylene-1,2-dimethanesulphonate and during subsequent Leydig cell regeneration.
Ethylene-1,2-dimethanesulphonate (EDS) rapidly destroys Leydig cells in the rat testis, although repopulation occurs within 5-7 weeks. In this study we have examined the activity of testicular steroidogenic enzymes after Leydig cell destruction and during regeneration. This was designed to measure the contribution of cells, other than Leydig cells, to steroidogenic activity in the testis, and to determine whether changes in steroidogenic enzyme activity during Leydig cell regeneration after EDS parallel those which occur during normal Leydig cell development. The enzymes studied are those responsible for androgen synthesis and metabolism in the testis. Adult male Wistar rats (300-350 g) were injected with EDS (100 mg/kg, i.p.) and testicular steroidogenic enzyme activity was measured on days 0, 3, 7, 14, 21 and 35. On day 3, when no Leydig cells remain in the testis, cholesterol side-chain cleavage (CSCC) activity, per testis, declined to undetectable levels, while 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) and 17 alpha-hydroxylase retained only 0.04 and 0.15% of control activity respectively. In contrast, 17-ketosteroid reductase (17-KSR) and 5 alpha-reductase retained 33 and 10% of control activity respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
O'Shaughnessy
P J
PJ
Department of Basic Sciences, Royal Veterinary College, London.
Murphy
L
L
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism; D000728:Androgens / Q000096:biosynthesis*; D000818:Animals; D002455:Cell Division / Q000502:physiology; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D008297:Male; D008698:Mesylates / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000201:enzymology*; D013997:Time Factors
D000728:Androgens; D008698:Mesylates; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases; D002786:Cholesterol Side-Chain Cleavage Enzyme; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; C002994:ethylene dimethanesulfonate