title: Heat-labile toxin-producing isolates of Pasteurella multocida from rabbits.
abstract: Five of one hundred forty seven isolates of Pasteurella multocida from rabbits were found to produce heat-labile toxin. Each isolate was assayed for the ability of potassium thiocyanate (KSCN) extracts to cause dermonecrosis in guinea pig skin, ability of bacteria or filtrates to cause cytotoxicity in cell cultures, and reactivity with monoclonal antibodies to heat-labile P. multocida toxin. Five capsular type D isolates produced dermonecrosis and reacted with monoclonal antibodies to toxin. Filtrates of all five of these isolates were cytotoxic for cell cultures. Potassium thiocyanate extracts of all five isolates caused pleuritis and pneumonia in rabbits after intranasal inoculation. Turbinate atrophy was seen in 5 of 19 rabbits inoculated intranasally with toxic extracts. Heat-labile toxin was not produced by 109 capsular type A isolates or 19 nontypable isolates.
authors.affiliation: Unit for Laboratory Animal Medicine, University of Michigan Medical School, Ann Arbor 48109.
authors.forename: M A
authors.initials: MA
authors.lastname: Suckow
authors.forename: C E
authors.initials: CE
authors.lastname: Chrisp
authors.forename: N T
authors.initials: NT
authors.lastname: Foged
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002417:Cattle; D002460:Cell Line; D003885:Dermotoxins / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000032:analysis; D008168:Lung / Q000196:embryology; D010326:Pasteurella Infections / Q000209:etiology / Q000382:microbiology* / Q000473:pathology; D016979:Pasteurella multocida / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits; D014709:Vero Cells
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D003885:Dermotoxins; D007074:Immunoglobulin G; C052804:dermonecrotic toxin, Pasteurella multocida