[Local structure of cytochrome c from horse heart in solution. Conformational analysis using data of two-dimensional nuclear Overhauser effect spectroscopy].
Using the earlier suggested method the calculation of the backbone conformations of horse heart cytochrome c in oxidized (ferricytochrome c) and reduced (ferrocytochrome c) states has been performed by the two-dimensional nuclear Overhauser effect spectroscopy data. For both protein forms the secondary structure elements have been revealed and the conformations of the irregular polypeptide chain segments have been analysed. The similarity of the secondary structures of ferri- and ferrocytochrome c in solution was established from the comparison of their conformations. Small differences between the conformations of two molecule forms are shown to be localized within the polypeptide chain fragments situated in the spatial structure near the heme crevice. The comparison of the dihedral phi and psi angles in the calculated conformations of horse cytochrome C with the corresponding characteristics of X-ray structures of tuna ferri- and ferrocytochrome c made for the oxidized and reduced protein forms using the quantitative criteria testifies the similarity of their conformations in solution and crystal. In is shown that the conformational changes of the separate amino acid residues which take place as the result of the "solution-to-crystal" transition occur on the surface fragments of protein globule and do not lead to essential alterations of the secondary molecule structure.
Andrianov
A M
AM
Akhrem
A A
AA
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003460:Crystallization; D003574:Cytochrome c Group / Q000737:chemistry*; D006736:Horses; D009682:Magnetic Resonance Spectroscopy; D009206:Myocardium / Q000201:enzymology*; D010084:Oxidation-Reduction; D011487:Protein Conformation; D014961:X-Ray Diffraction
D000596:Amino Acids; D003574:Cytochrome c Group
Lokal'naia struktura tsitokhroma c iz serdtsa loshadi v rastvore. Konformatsionnyĭ analiz po dannym dvumernoĭ spektroskopii iadernogo éffekta overkhauzera.