Sequence of a staphylococcal plasmid gene, vga, encoding a putative ATP-binding protein involved in resistance to virginiamycin A-like antibiotics.
The Staphylococcus aureus plasmid gene, vga, conferring resistance to A compounds of virginiamycin-like antibiotics (streptogramin A, pristinamycin II, virginiamycin M), and to synergistic mixtures of the A and B compounds of these antibiotics, was cloned and sequenced. This gene potentially encodes a 522-amino acid protein, VgA, of 60,115 Da which exhibits significant homology with the ATP-binding domains of numerous proteins. VgA has two ATP-binding domains, containing each the A and the B motifs, but does not contain long hydrophobic stretches that might represent potential membrane-spanning domains. Resistance to A compounds of virginiamycin-like antibiotics conferred to S. aureus by gene vga was not conferred to Escherichia coli, although a protein having an M(r) similar to that encoded by this gene was detected in E. coli minicells.
Allignet
J
J
National Reference Center for Staphylococci, Institut Pasteur, Paris, France.
Loncle
V
V
el Sohl
N
N
D018528:ATP-Binding Cassette Transporters; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D011485:Protein Binding; D015183:Restriction Mapping; D013211:Staphylococcus aureus / Q000235:genetics*; D014769:Virginiamycin / Q000494:pharmacology*
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D014769:Virginiamycin; C075964:VgA protein, Staphylococcus aureus; D000255:Adenosine Triphosphate