Oligonucleotide probes for the specific detection of the wild poliovirus types 1 and 3 endemic to Brazil.
Synthetic oligodeoxynucleotide probes, 21-23 nucleotides in length, were prepared which specifically hybridize to the genomes of the wild type 1 and 3 polioviruses currently endemic to the northeastern region of Brazil. The probes are complementary to sequences near the 5'-terminus of the VP1 gene that differ substantially among genetically distant polioviruses but are largely conserved among related isolates. The probes have been routinely used in the laboratory surveillance of poliomyelitis cases in Brazil, permitting direct, rapid identification of the indigenous wild polioviruses by dot-blot hybridization.
da Silva
E E
EE
Departamento de Virologia, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Pallansch
M A
MA
Holloway
B P
BP
Oliveira
M J
MJ
Schatzmayr
H G
HG
Kew
O M
OM
D000595:Amino Acid Sequence; D001483:Base Sequence; D001938:Brazil; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D011051:Poliomyelitis / Q000175:diagnosis / Q000382:microbiology*; D017955:Poliovirus / Q000235:genetics / Q000302:isolation & purification*; D011159:Population Surveillance; D012367:RNA, Viral / Q000235:genetics
D015345:Oligonucleotide Probes; D012367:RNA, Viral