Cloning of murine splenic T lymphocytes and natural killer (NK) cells on filter paper discs: detection of a novel NK/T phenotype.
Discrete colonies of splenocytes were grown on filter paper discs in the presence of concanavalin A and interleukin 2. Phenotypic analysis of the colonies indicated that the majority expressed the Thy-1.2 marker and 72% of these co-expressed the CD3 molecule. Of the colonies 20%-25% were NK 1.1+ and they developed regardless of the presence of Con A in the culture medium, a property of the NK lineage. In addition, Thy-1.2+ colonies developed when splenocytes from scid mice, which lack mature T and B cells, were grown both in the presence and absence of concanavalin A. These results demonstrate that colonies of murine splenic T lymphocytes and NK cells could be successfully grown on filter paper discs and phenotypically characterized. With this colonies technique, it was possible to identify a novel subset of NK 1.1+ colonies that co-expresses CD3 and shares growth properties with T cell colonies.
Sarzotti
M
M
Department of Veterans Affairs Medical Center, Baltimore, MD 21218.
Kumar
V
V
Bennett
M
M
Kelsoe
G
G
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D000954:Antigens, Surface / Q000276:immunology* / Q000378:metabolism; D017252:CD3 Complex; D002999:Clone Cells; D003208:Concanavalin A / Q000494:pharmacology; D005374:Filtration / Q000295:instrumentation; D007153:Immunologic Deficiency Syndromes / Q000276:immunology; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D018800:Thy-1 Antigens
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D017252:CD3 Complex; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D018800:Thy-1 Antigens; D003208:Concanavalin A