title: [Evidence for Campbell's mechanism for fine excision of Tn9 type transposons in Escherichia coli K12].
abstract: The plasmid-transposon Tn9-322 was constructed by inverted transposition from the pBR322::Tn9 plasmid. The precise excision of the Tn9-322 transposon from the proB gene site can proceed by the Campbell's model. This fact was demonstrated by appearance of the plasmid-transposons after their precise excision. They contain two IS1 elements flanking a short direct repeat of the target DNA. The recombinational mechanism of precise excision of Tn9 type transposons seems not to be alternative but looks as an additional one to a well-known slippage mechanism proved for Tn5 and Tn10.
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Goryshin
authors.forename: S E
authors.initials: SE
authors.lastname: Tamm
authors.forename: V A
authors.initials: VA
authors.lastname: Lantsov
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial
vernacular_title: Dokazatel'stvo kempbellovskogo mekhanizma tochnoĭ ékstsizii sostavnogo transpozona tipa Tn9 Escherichia coli K12.