A single-step DNA extraction procedure for the detection of serum hepatitis B virus sequences by the polymerase chain reaction.
A rapid single-step procedure for the isolation of low molecular weight DNA using guanidinium thiocyanate and phenol as protein denaturants is described and applied for the detection of specific hepatitis B virus (HBV) DNA sequences from serum samples by the polymerase chain reaction (PCR). The novel technique is efficient and, when compared to the standard proteinase K/phenol/chloroform method has the advantage of being faster and easily adaptable to the routine processing of a high number of clinical samples by PCR and spot hybridization techniques.
Manzin
A
A
Institute of Microbiology, University of Ancona Medical School, Ancona, Italy.
Salvoni
G
G
Bagnarelli
P
P
Menzo
S
S
Carloni
G
G
Clementi
M
M
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D004279:DNA, Viral / Q000097:blood / Q000302:isolation & purification*; D006509:Hepatitis B / Q000382:microbiology; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D004279:DNA, Viral