[Oxidative damage to E. coli membranes caused by superoxide radicals].
The effects of bacterial membranes of active oxygen species photochemically generated by riboflavin-histidine systems were studied. According to SDS-PAAG data, the formation of high molecular weight protein aggregates and the appearance of fluorochromes whose fluorescence is seen in the longwave length region of the spectrum (lambda excit = 350 nm, lambda emis = 400-500 nm) and which are bound to the proteins, are suggestive of membrane oxidation consisting in the chemical modification of protein components. The presence in E. coli membranes of endogenous photosensitizers which upon illumination with visible light induce the oxidation of membrane proteins, was established.
Sharonov
B P
BP
Kondrat'eva
L D
LD
D002462:Cell Membrane / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects*; D005454:Fluorescence Polarization; D005609:Free Radicals; D008565:Membrane Proteins / Q000378:metabolism; D010084:Oxidation-Reduction; D013481:Superoxides / Q000494:pharmacology*
D005609:Free Radicals; D008565:Membrane Proteins; D013481:Superoxides
Okislitel'noe povrezhdenie membran E. coli, vyzyvaemoe superoksidnymi radikalami.