Phorbol ester induces phosphorylation and down-regulation of connexin 43 in WB cells.
Western blot analysis indicated that WB cells contained multiple proteins of molecular mass 42-47 K which reacted with anti-connexin 43 antibody. Incubation of cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) for 5 min caused the inhibition of dye transfer and the appearance of reactive material of 50 K which was abolished by acid phosphatase. Longer term incubation with TPA caused loss of connexin 43 protein.
Oh
S Y
SY
School of Biological Sciences, Flinders University, Adelaide, Australia.
Grupen
C G
CG
Murray
A W
AW
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D017630:Connexins; D003513:Cycloheximide / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D066298:In Vitro Techniques; D007365:Intercellular Junctions / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D017630:Connexins; D008565:Membrane Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate