title: 2-amino-4-phosphonobutyric acid exerts a light-dependent effect on post-gabaculine levels of retinal gamma-aminobutyric acid (GABA): evidence that ON synaptic pathways regulate retinal GABAergic transmission.
abstract: The effects of light, 2-amino-4-phosphonobutyric acid (APB), and kainic acid on rat retinal gamma-aminobutyric acid (GABA)-ergic transmission were studied by measuring levels of retinal GABA following subcutaneous injection of gabaculine, an irreversible inhibitor of GABA-transaminase. Post-gabaculine levels of retinal GABA in light-exposed rats were significantly greater than those in rats held in darkness. The synaptic mechanism of this effect of light was examined by measuring post-gabaculine levels of retinal GABA in rats placed into either lighted or darkened conditions after receiving unilateral intravitreal injections of APB, a glutamate analogue that selectively decreases the activity of ON synaptic pathways in the retina. APB attenuated the post-gabaculine accumulation of GABA in rats held in the light, but not in those placed into darkness. Furthermore, the light-dependent increment in post-gabaculine accumulation of retinal GABA was entirely APB sensitive, and the effect of APB was entirely light dependent. In contrast to APB, kainic acid stimulated the post-gabaculine accumulation of retinal GABA in vivo. Our findings suggest that APB and kainic acid influence GABAergic transmission at different sites in the retina and that some retinal GABAergic neurons are either ON or ON-OFF amacrine cells.
authors.affiliation: Department of Neuroscience, Albert Einstein College of Medicine, New York, NY 10461.
authors.forename: J F
authors.initials: JF
authors.lastname: Cubells
authors.forename: C
authors.initials: C
authors.lastname: Ndubuka
authors.forename: M H
authors.initials: MH
authors.lastname: Makman
mesh_terms: D000613:Aminobutyrates / Q000494:pharmacology*; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology*; D007608:Kainic Acid / Q000494:pharmacology; D008027:Light; D008297:Male; D009118:Muscimol / Q000494:pharmacology; D009434:Neural Pathways / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000378:metabolism*; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission; D014701:Veratridine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000502:physiology
chemical_list: D000613:Aminobutyrates; D003509:Cyclohexanecarboxylic Acids; D009118:Muscimol; C012987:gabaculine; D005680:gamma-Aminobutyric Acid; D014701:Veratridine; C012729:2-amino-4-phosphonobutyric acid; D007608:Kainic Acid; D001640:Bicuculline