title: HuD, a paraneoplastic encephalomyelitis antigen, contains RNA-binding domains and is homologous to Elav and Sex-lethal.
abstract: A neuronal antigen (HuD) recognized by the sera of patients with antibody-associated paraneoplastic encephalomyelitis has been isolated by screening a lambda cerebellar expression library. The recombinant antigen provides an unambiguous assay for this rare condition associated with small cell lung cancer. The recombinant antigen has been used to identify specific infiltrating lymphocytes in tumors and affected brain tissues of patients with antibody-associated paraneoplastic encephalomyelitis and sensory neuronopathy. HuD mRNA is uniquely expressed in brain tissue. The HuD protein shows a remarkable homology to the Drosophila proteins Elav and Sex-lethal and is likely to play a role in neuron-specific RNA processing.
authors.affiliation: Laboratory of Molecular Neuro-Oncology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
authors.forename: A
authors.initials: A
authors.lastname: Szabo
authors.forename: J
authors.initials: J
authors.lastname: Dalmau
authors.forename: G
authors.initials: G
authors.lastname: Manley
authors.forename: M
authors.initials: M
authors.lastname: Rosenfeld
authors.forename: E
authors.initials: E
authors.lastname: Wong
authors.forename: J
authors.initials: J
authors.lastname: Henson
authors.forename: J B
authors.initials: JB
authors.lastname: Posner
authors.forename: H M
authors.initials: HM
authors.lastname: Furneaux
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D001921:Brain / Q000276:immunology; D018288:Carcinoma, Small Cell / Q000276:immunology; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; D004679:Encephalomyelitis / Q000276:immunology*; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000276:immunology; D010257:Paraneoplastic Syndromes / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000951:Antigens, Neoplasm; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; C498010:ELAVL4 protein, human; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins