Suppression of antibody response to an unrelated antigen in experimental murine paracoccidioidomycosis: effect of cyclophosphamide and indomethacin.
A suppressive effect of experimental paracoccidioidomycosis on the IgE antibody response to an unrelated antigen (ovalbumin) has been previously observed in mice. This effect was restricted to a short period, reaching maximum levels when OA was administered on the third day of Pb-infection. In order to study possible mechanisms involved in the establishment of this suppression, resistant (A/SN) and susceptible (B10.A) mice were treated with either a low dose of cyclophosphamide (CY) or indomethacin (INDO), a potent inhibitor of prostaglandin synthesis. While treatment with the first drug in A/SN mice induced only a recovery of the IgE anti-OA antibody response, in B10.A mice this effect was extended to IgG1, IgG2a and total levels of anti-OA antibodies. On the other hand, treatment with INDO reverted the anti-OA antibody suppression regarding each antibody class tested and in both strains of mice. These results suggest the participation of prostaglandins and of a cyclophosphamide-sensitive mechanism in the induction of the suppressive phenomenon in the human system.
Amarante-Mendes
J G
JG
Department of Immunology, University of São Paulo, Brazil.
Ferreira
A P
AP
Macedo
M S
MS
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000941:Antigens; D003520:Cyclophosphamide / Q000494:pharmacology*; D005260:Female; D007108:Immune Tolerance / Q000187:drug effects; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007213:Indomethacin / Q000494:pharmacology*; D051379:Mice; D008805:Mice, Inbred A; D010047:Ovalbumin / Q000276:immunology; D010229:Paracoccidioidomycosis / Q000276:immunology*; D013997:Time Factors
D000941:Antigens; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D003520:Cyclophosphamide; D010047:Ovalbumin; D007213:Indomethacin