title: Large-scale purification of inactivated hepatitis A virus by centrifugation in non-ionic gradients.
abstract: Formalin-inactivated hepatitis A virus (HAV) can be purified for vaccine preparation by centrifugation in Renografin-76 (diatrizoate meglumine and diatrizoate sodium) gradients. Both continuous-flow rate-zonal and isopycnic methods were used for the separation of a major antigen component from minor antigen and host protein. The major antigen component, which appeared to contain complete virions by electron microscopy, could be recovered from gradients and accounted for approximately one third of the total antigen in the starting material. The HAV-specific purified antigen could be enriched 200-300-fold by either centrifugation procedure. The purified HAV antigen, when adsorbed to alum and inoculated into mice, was found to be highly immunogenic.
authors.affiliation: Division of Communicable Disease and Immunology, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
authors.forename: D R
authors.initials: DR
authors.lastname: Dubois
authors.forename: K H
authors.initials: KH
authors.lastname: Eckels
authors.forename: J
authors.initials: J
authors.lastname: Ticehurst
authors.forename: L N
authors.initials: LN
authors.lastname: Binn
authors.forename: R L
authors.initials: RL
authors.lastname: Timchak
authors.forename: D A
authors.initials: DA
authors.lastname: Barvir
authors.forename: C T
authors.initials: CT
authors.lastname: Rankin
authors.forename: S P
authors.initials: SP
authors.lastname: O'Neill
mesh_terms: D000956:Antigens, Viral / Q000032:analysis; D002499:Centrifugation, Density Gradient / Q000379:methods*; D006507:Hepatovirus / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D011863:Radioimmunoassay; D014765:Viral Vaccines; D014775:Virus Activation
chemical_list: D000956:Antigens, Viral; D014765:Viral Vaccines