Crystallization and preliminary X-ray diffraction study of cytochrome c552 from Hydrogenobacter thermophilus.
Cytochrome c552 from a thermophilic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus, exhibits remarkable thermostability. The oxidized cytochrome c552 has been crystallized in an ethanol/water mixture by means of the vapor diffusion method. The crystals belong to the orthorhombic system, space group P2(1)2(1)2, with unit cell dimensions of a = 93.4 A, b = 52.9 A, and c = 32.4 A. Most probably the asymmetric unit contains two molecules of cytochrome c552. The crystals diffract X-rays to better than 2.5 A resolution and are stable to X-ray irradiation.
Fukuyama
K
K
Department of Biology, Faculty of Science, Osaka University.
Matsubara
H
H
Sanbongi
Y
Y
Kodama
T
T
D001420:Bacteria, Aerobic / Q000737:chemistry*; D003460:Crystallization; D003574:Cytochrome c Group / Q000737:chemistry*; D006358:Hot Temperature; D011487:Protein Conformation; D014961:X-Ray Diffraction
D003574:Cytochrome c Group; C083951:cytochrome c553; C022302:cytochrome C-552