Cloning, pharmacological characteristics and expression pattern of the rat GABAA receptor alpha 4 subunit.
A cDNA of rat brain encoding the GABAA receptor alpha 4 subunit has been cloned. Recombinant receptors composed of alpha 4, beta 2 and gamma 2 subunit bind with high affinity the GABA agonist [3H]muscimol and the benzodiazepine 'alcohol antagonist' [3H]Ro 15-4513, but fail to bind benzodiazepine agonists. The alpha 4 subunit is expressed mainly in the thalamus, as assessed by in situ hybridization histochemistry, and may participate in a major population of thalamic GABAA receptors. The alpha 4 mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum.
Wisden
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W
Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Heidelberg, Germany.
Herb
A
A
Wieland
H
H
Keinänen
K
K
Lüddens
H
H
Seeburg
P H
PH
D000345:Affinity Labels / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001386:Azides / Q000378:metabolism; D001483:Base Sequence; D001569:Benzodiazepines / Q000378:metabolism; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009118:Muscimol / Q000378:metabolism; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011963:Receptors, GABA-A / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism
D000345:Affinity Labels; D001386:Azides; C546262:Gabra4 protein, rat; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D011994:Recombinant Proteins; D001569:Benzodiazepines; D009118:Muscimol; C042957:Ro 15-4513