title: Bactericidal activity of daptomycin against vancomycin-resistant Enterococcus faecium in an in vitro pharmacokinetic model.
abstract: A dynamic in vitro model was used to determine the killing kinetics of daptomycin against 15 vancomycin-resistant clinical isolates of Enterococcus faecium. Concentration profiles simulating those observed in serum following administration of both low-dose (2 mg/kg) and high-dose (6 mg/kg) daptomycin were bactericidal within 5.5 and 2.8 h, respectively. In contrast, when albumin was added to the growth medium, the corresponding bacterial killing times were slowed to greater than 24 h and 7 h; these results suggest that in the clinical setting, daptomycin dosages of approximately 6 mg/kg are required to achieve bactericidal activity against vancomycin-resistant Enterococcus faecium.
authors.affiliation: Laboratoire de Microbiologie, Hôpital Robert Debré, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Bingen
authors.forename: C
authors.initials: C
authors.lastname: Doit
authors.forename: N
authors.initials: N
authors.lastname: Lambert-Zechovsky
authors.forename: M
authors.initials: M
authors.lastname: Tod
authors.forename: O
authors.initials: O
authors.lastname: Petitjean
authors.forename: F
authors.initials: F
authors.lastname: Bourgeois
authors.forename: P
authors.initials: P
authors.lastname: Mariani-Kurkdjian
mesh_terms: D000418:Albumins / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000097:blood / Q000494:pharmacology*; D017576:Daptomycin; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial; D016984:Enterococcus faecium / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D010455:Peptides / Q000097:blood / Q000494:pharmacology; D015610:Serum Bactericidal Test; D014640:Vancomycin / Q000097:blood / Q000494:pharmacology*
chemical_list: D000418:Albumins; D000900:Anti-Bacterial Agents; D010455:Peptides; D014640:Vancomycin; D017576:Daptomycin