Structural characterization of a novel glycosyl-phosphatidylinositol from the protozoan Tetrahymena mimbres.
A glycolipid metabolically labelled with [14C]GlcN was isolated from the free-living protozoan Tetrahymena mimbres. The glycolipid was sensitive to a bacterial phosphatidylinositol-specific phospholipase C, and the headgroup was shown to contain a phosphorylated Man alpha 1-2Man alpha 1-4Man alpha 1-4GlcN glycan. The Tetrahymena glycolipid is structurally unique among the glycosylphosphatidylinositols that have so far been characterized, including those from several protozoan parasites of humans.
Weinhart
U
U
Department of Biochemistry, University of Dundee, Scotland, U.K.
Thomas
J R
JR
Pak
Y B
YB
Thompson
G A
GA
Ferguson
M A
MA
D000818:Animals; D002240:Carbohydrate Sequence; D006017:Glycolipids / Q000737:chemistry* / Q000378:metabolism; D017261:Glycosylphosphatidylinositols; D008969:Molecular Sequence Data; D015394:Molecular Structure; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000737:chemistry* / Q000378:metabolism; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013768:Tetrahymena / Q000737:chemistry*
D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D010716:Phosphatidylinositols; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase