title: [Obtaining monoclonal antibodies to paired tangled filaments from the brain of patients with Alzheimer's disease].
abstract: Hybridoma technique was used to obtain a clone producing IgG antibodies. Paired helical neurofilaments isolated from the brain of patients suffering from Alzheimer's disease were used as an antigen during preparation of the clone. Antibodies recognize neurons with neurofibrillary changes. Specificity of the obtained antibodies to paired helical filaments was proved immunohistochemically. Monoclonal antibodies obtained are likely to be useful in the determination of the role played by pathological proteins in mutually twisted filaments formation.
authors.forename: M M
authors.initials: MM
authors.lastname: Chumakova
authors.forename: V M
authors.initials: VM
authors.lastname: Vostrikov
authors.forename: N N
authors.initials: NN
authors.lastname: Dergunova
authors.forename: E A
authors.initials: EA
authors.lastname: Rogaeva
mesh_terms: D000544:Alzheimer Disease / Q000276:immunology / Q000473:pathology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002540:Cerebral Cortex / Q000276:immunology / Q000648:ultrastructure*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000473:pathology; D016874:Neurofibrillary Tangles / Q000276:immunology*; D013154:Spleen / Q000166:cytology
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G
vernacular_title: Poluchenie monoklonal'nykh antitel k poparno skuchennym filamentam iz mozga bol'nykh bolezn'iu Al'tsgeĭmera.