Fixed-cell immunoperoxidase technique for the study of surface antigens induced by the coronavirus of transmissible gastroenteritis (TGEV).
An immunoperoxidase technique performed on the TGEV-infected cells was developed for detection of virus-induced antigens. The presence of M antigen of TGEV on the surface of infected cells was demonstrated by this technique. This finding is in contrast to the M protein of murine hepatitis coronavirus which migrates to the Golgi apparatus but is not transported to the plasma membrane. The time course of appearance M and S antigens on the surface of TGEV-infected cell can be studied by this technique.
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Laboratory of Virology, Institute of Veterinary Research, Bach mai-Hànôi, Viêtnam.
Bernard
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Lantier
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D000818:Animals; D000954:Antigens, Surface / Q000032:analysis / Q000096:biosynthesis; D000956:Antigens, Viral / Q000032:analysis* / Q000096:biosynthesis; D002460:Cell Line; D007124:Immunoenzyme Techniques; D007700:Kinetics; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000032:analysis / Q000096:biosynthesis; D015678:Viral Structural Proteins / Q000032:analysis / Q000096:biosynthesis
D000954:Antigens, Surface; D000956:Antigens, Viral; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins