title: An atypical case of cytochrome c oxidase deficiency with biochemical heterogeneity in fibroblasts.
abstract: A patient presenting in the first year of life with feeding difficulties and failure to grow had variable but persistent lactic acidemia noted at age 20 months. Nonspecific nutritional and biochemical therapy was accompanied by improvement in general clinical status, growth, gait, and development. However, she died in a catastrophic illness at the end of the third year of life. Studies in intact fibroblast mitochondria were consistent with an isolated but partial defect in cytochrome c oxidase. On direct assay of this enzyme complex in fibroblast homogenates and mitochondria, activity was much more severely depressed (less than or equal to 8% of control). Her fibroblasts normally synthesized the three cytochrome c oxidase subunits encoded on the mitochondrial genome. These data confirm that this patient had cytochrome c oxidase deficiency and demonstrate significant biochemical heterogeneity, since the results of the intact mitochondrial studies correlate better with her clinical course than do those of the direct enzymatic assays.
authors.affiliation: Department of Pediatrics, College of Medicine, University of Oklahoma Health Science Center, Oklahoma City.
authors.forename: R
authors.initials: R
authors.lastname: Lutz
authors.forename: A
authors.initials: A
authors.lastname: Garnica
authors.forename: A
authors.initials: A
authors.lastname: Shires
authors.forename: E
authors.initials: E
authors.lastname: Freneaux
authors.forename: D
authors.initials: D
authors.lastname: De Vivo
authors.forename: P
authors.initials: P
authors.lastname: Neuhoff
authors.forename: W J
authors.initials: WJ
authors.lastname: Rhead
mesh_terms: D002478:Cells, Cultured; D002675:Child, Preschool; D030401:Cytochrome-c Oxidase Deficiency; D004591:Electrophoresis, Polyacrylamide Gel; D005183:Failure to Thrive / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008928:Mitochondria / Q000378:metabolism
chemical_list: D007773:Lactates; D019344:Lactic Acid