Adsorption to hydroxyapatite of partially deglycosylated human salivary mucins in competition with phosvitin and phytate.
The effect of phosvitin and phytate on the binding of native as well as partially deglycosylated human whole salivary mucins (HWSM) to hydroxyapatite was studied. Native HWSM preadsorbed onto hydroxyapatite was completely desorbed in the presence of greater than 500 micrograms/ml phosvitin. In contrast, in similar experiments, asialo-HWSM was desorbed approximately 10%. Desorption of preadsorbed asialo-afuco-HWSM in the presence of 1 mg/ml phosvitin was approximately 20%. Further deglycosylation of HWSM resulted in preparations which, after preadsorption to hydroxyapatite, were not desorbed upon subsequent incubation with phosvitin. With phytate, a less effective competitor of HWSM for the hydroxyapatite surface, essentially the same results were obtained, i.e. increase in deglycosylation of HWSM was concomitant with decrease in desorption by phytate. Using other incubation conditions (preadsorption of a phosphocompound, and simultaneous incubation of HWSM and phosphocompounds) essentially the same conclusion was obtained. The data indicate that the ability of salivary mucins to absorb to hydroxyapatite in competition with phosphocompounds appears to be enhanced by deglycosylation.
Nieuw Amerongen
A V
AV
Academic Centre for Dentistry Amsterdam (ACTA), Department of Oral Biochemistry, Vrije Universiteit.
Oderkerk
C H
CH
Veerman
E C
EC
D001212:Asialoglycoproteins; D001667:Binding, Competitive; D017886:Durapatite; D006031:Glycosylation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites / Q000378:metabolism*; D009077:Mucins / Q000378:metabolism*; D010774:Phosvitin / Q000378:metabolism* / Q000494:pharmacology; D010833:Phytic Acid / Q000378:metabolism* / Q000494:pharmacology; D012471:Salivary Proteins and Peptides / Q000378:metabolism
D001212:Asialoglycoproteins; D006882:Hydroxyapatites; D009077:Mucins; D012471:Salivary Proteins and Peptides; D010833:Phytic Acid; D010774:Phosvitin; D017886:Durapatite