title: Endocytosis of gold-labeled proteins and LDL by Trypanosoma cruzi.
abstract: Endocytosis was studied at the ultrastructural level in different developmental forms of Trypanosoma cruzi after incubation of the parasites in the presence of gold-labeled proteins (albumin-Au, peroxidase-Au and transferrin-Au) and low-density lipoprotein (LDL-Au). Epimastigote (culture) forms actively ingested LDL and proteins. Initially, gold particles were seen adhering only to the cytostome and inside the flagellar pocket. In parasites incubated at 4 degrees C with transferrin-Au or peroxidase-Au, labeling was found only at these two sites, showing that receptor-mediated endocytosis occurs in both regions. In the cytoplasm, gold particles were seen only inside two different compartments: membrane-bound vesicles and reservosomes. Incubation of epimastigotes with acridine orange followed by fluorescence microscopy revealed intense orange staining, indicating that the reservosomes have an acidic pH. This staining was abolished after incubation of the parasites in the presence of ammonium chloride. These data confirm that this compartment is the site of accumulation of ingested lipids and proteins. Little intracellular labeling with transferrin-Au was found in in vitro-derived amastigotes and trypomastigotes (both lack reservosomes). However, although in amastigotes very few gold particles were seen bound to the cells, in trypomastigotes they were observed bound to the membrane that encloses the cell body, the flagellar pocket, and the flagellum, suggesting that the receptors are more abundant in this form.
authors.affiliation: Departamento de Parasitologia e Biofisica Celular, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: M J
authors.initials: MJ
authors.lastname: Soares
authors.forename: W
authors.initials: W
authors.lastname: de Souza
mesh_terms: D000418:Albumins / Q000378:metabolism; D000818:Animals; D004705:Endocytosis; D006046:Gold; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009195:Peroxidase / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000418:Albumins; D008077:Lipoproteins, LDL; D011506:Proteins; D014168:Transferrin; D006046:Gold; D009195:Peroxidase