title: Invasion and survival of Fusarium solani in the dexamethasone-treated cornea of rabbits.
abstract: Electron microscopic observations, as well as in vitro experiments, on experimental Fusarium solani keratitis of rabbits were performed to study the mode of fungal invasion into the corneal stroma, the interactions between F. solani and inflammatory cells under the influence of topical dexamethasone (DXM) treatment, and the survival mechanism of the fungi in the DXM-treated cornea. Electron microscopy showed that, while the fungus invaded into the corneal stroma, digestion of collagen fibrils occurred around the hyphae, where amorphous material was often noted. In DXM-nontreated cornea, the fungal hyphae were entrapped by pseudopodia of the neutrophils and destruction of the hyphae was noted on day 3 of infection, most hyphae having disappeared by day 7. In the DXM-treated cornea, however, neutrophils could not ingest and destroy the hyphae. In qualitative nitroblue tetrazolium (NBT)-reduction tests using rabbit peripheral blood neutrophils, DXM significantly suppressed the rate of NBT-reduction and the rate of adherence to the fungal microconidia. In the DXM-treated corneal lesions, a considerable increase in both number and size of fungal peroxisomes was noted. Furthermore, the hyphae, surrounded by neutrophils, showed double or triple cell wall formation or sometimes a hypha-in-hypha structure. Similar hypha-in-hypha structures were also observed when the organisms were treated in vitro with a fungistatic concentration of H2O2. We suggest that this special structure is a protective device produced for the survival of F. solani when subject to neutrophil attack in the DXM-treated cornea.
authors.affiliation: Department of Dermatology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kiryu
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Suenaga
authors.forename: M
authors.initials: M
authors.lastname: Asahi
mesh_terms: D000818:Animals; D003315:Cornea / Q000187:drug effects / Q000382:microbiology*; D003907:Dexamethasone / Q000494:pharmacology*; D015821:Eye Infections, Fungal / Q000276:immunology / Q000382:microbiology*; D005260:Female; D005670:Fusarium / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007634:Keratitis / Q000276:immunology / Q000382:microbiology*; D008297:Male; D008854:Microscopy, Electron; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D009580:Nitroblue Tetrazolium; D011817:Rabbits; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides; D009580:Nitroblue Tetrazolium; D003907:Dexamethasone; D006861:Hydrogen Peroxide