An unusually proximal deletion on the short arm of chromosome 3 in a patient with small cell lung cancer.
The tumors of patients with small cell lung carcinoma (SCLC) frequently exhibit the loss of alleles at polymorphic loci on the short arm of chromosome 3. We report the genotype analysis of six SCLC patients obtained using 15 chromosome 3 probes that identified 19 restriction fragment length polymorphisms (RFLPs). Five of the six patients were reduced to homozygosity in the tumor DNA at every informative 3p locus, and thus did not serve to delineate the deletion. However, the RFLP analysis of the tumor DNA of the sixth patient demonstrated both heterozygous and hemizygous loci on 3p and allowed the definition of an interstitial deletion that extends proximal to the D3S2 locus at 3p14.2-p21 to include at least 3p13-p14. The exclusion of the D3F15S2 locus from the deleted region, observed in this patient, is an uncharacteristic feature of SCLC deletions. This deletion includes the location of D3S30 and D3S4, and thus serves to map these loci within the proximal half of chromosome 3.
Daly
M C
MC
MRC Clinical Oncology and Radiotherapeutics Unit, MRC Centre, Cambridge, United Kingdom.
Douglas
J B
JB
Bleehen
N M
NM
Hastleton
P
P
Twentyman
P R
PR
Sundaresan
V
V
Carritt
B
B
Bergh
J
J
Rabbitts
P H
PH
D000483:Alleles; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D003720:Densitometry; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014407:Tumor Cells, Cultured