Evaluation of lymphocyte activation by flow cytometric determination of interleukin-2 (CD25) receptor.
Lymphocyte response to mitogens is a habitually-used test in the evaluation of the immune system. The usual method with [3H]thymidine yields results which are difficult to interpret. In our search for an alternative, we examined the feasibility of determining T lymphocyte activation by measuring interleukin-2 receptor (CD25) expression with flow cytometry. We found a good correlation between CD25 molecule expression and the dose of phytohemagglutinin, however, there was no correlation between disintegrations per minute produced by [3H]thymidine uptake and the percentage of CD25 expressed by cells.
Santos
J L
JL
Department of Biochemistry and Molecular Biology, University of Granada, Spain.
Montes
M J
MJ
Garcia-Pacheco
M
M
Gonzalez
M R
MR
Gutierrez
F
F
D000328:Adult; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D010835:Phytohemagglutinins / Q000008:administration & dosage; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D013936:Thymidine / Q000378:metabolism
D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2; D013936:Thymidine