The use of labeled fusion protein for detection of B19 parvovirus IgM antibodies by an immunocapture test.
A new anti-B19 IgM ELISA was developed taking advantage of antibody-capture with biotinylated fusion protein as antigen. Specificity was examined using serum IgM antibody positive for rubella, hepatitis B core antigen, cytomegalovirus and Epstein-Barr virus as well as with sera positive for rheumatoid factors or antinuclear antibodies. The specificity was found to be 96%. Of one hundred serum samples compared using the new ELISA or the standard MACRIA tests for the presence of B19 IgM, 88 gave the same results. Fifty-three were negative and 35 were positive. Six sera were ELISA-negative MACRIA-positive, and six MACRIA-negative ELISA-positive. Thus, the ELISA gave 90% agreement with MACRIA. In a clinical study with 725 sera from suspected B19 infections, 161 (22%) were found positive by ELISA. The positive sera were from patients suffering from arthritis (35%), rash (35%), acute or chronic erythroblastopenia (21%), pancytopenia (5%), vascular purpura (2%) and lymphadenopathy (2%). A series of serum specimens obtained from two-B19 infected individuals were also studied. The IgM antibody became undetectable after four months.
Morinet
F
F
Hôpital Saint-Louis, Université Paris VII, Service de Bactériologie-Virologie, France.
Courouce
A M
AM
Galibert
F
F
Perol
Y
Y
D000914:Antibodies, Viral / Q000097:blood*; D001426:Bacterial Proteins; D015153:Blotting, Western; D002213:Capsid / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007075:Immunoglobulin M / Q000032:analysis*; D010321:Parvoviridae / Q000276:immunology*; D010322:Parvoviridae Infections / Q000175:diagnosis*; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D019809:Streptavidin
D000914:Antibodies, Viral; D001426:Bacterial Proteins; D007075:Immunoglobulin M; D011993:Recombinant Fusion Proteins; D019809:Streptavidin