Bovine viral diarrhea virus proteins: relatedness of p175 with p80 and p125 and evidence of glycoprotein processing.
Three monoclonal antibodies, which recognized two nonoverlapping antigenic domains and were reactive to the bovine viral diarrhea virus (BVDV) p80 protein, were found to cross react with the p125 protein of both cytopathic and noncytopathic BVDVs and a molecular weight 175,000 BVDV protein (p175). Results from limited proteolysis and chemical cleavage experiments confirmed the relatedness of these three proteins. In pulse-chase experiments it was apparent that p175 was a transient protein, as it was diminished during the chase, with a half-life of about 30 min. However, both p125 and p80 were also observed in short-pulsed lysates. Furthermore, during the chase, radiolabel was not found to accumulate into p125 or p80. Rather, these two proteins were stable with half-lives greater than 2 h. A fourth nonglycosylated protein, p37, increased during the chase. Processing of several glycoproteins was evident in these experiments. A glycoprotein of molecular weight 75,000 (gp75) diminished during the chase period, while glycoproteins gp62, gp48, and gp25 appeared or increased during the chase period. In contrast, the glycoprotein gp53 was a major protein in pulse-labeled cell lysates and remained constant throughout the chase period. In further experiments two stable forms of p80 differing in intramolecular disulphide bonding were observed.
Deregt
D
D
Animal Diseases Research Institute, Lethbridge, Alta., Canada.
Masri
S A
SA
Cho
H J
HJ
Ohmann
H B
HB
D000911:Antibodies, Monoclonal / Q000276:immunology; D001667:Binding, Competitive; D003429:Cross Reactions; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D014764:Viral Proteins