In vitro testing of a potentially biocompatible continuous ambulatory peritoneal dialysis fluid.
In the present study a newly formulated dialysis solution (HDF) was tested for its effects on phagocyte viability and function as well as its ability to support bacterial growth. This solution differs from standard CAPD solution (NPD) by the inclusion of histidine to buffer the fluid to pH 6.7. Low-dextrose HDF (1.36% w/v dextrose) did not significantly decrease the viability or inhibit any of the PMN functional parameters measured (phagocytosis, LTB4 release or respiratory burst activation) when compared to control buffer. NPD (1.36% w/v dextrose) at low pH as well as all high-dextrose dialysis solutions (NPD and HDF) significantly inhibited most PMN functions independently of reduced viability. Peritoneal mesothelial cell viability was unaffected by either low- or high-dextrose HDF but was significantly reduced by NPD (1.36 and 3.86% dextrose) at pH 5.2. The inhibitory effects of low dextrose dialysis solutions were confirmed as being partly related to their low initial pH. High-dextrose dialysis fluids, however, inhibit PMN function by a mechanism which, in addition to initial pH, appears to be directly related to their dextrose content but not their osmolality.
Topley
N
N
University of Wales College of Medicine, Institute of Nephrology, Cardiff Royal Infirmary, UK.
Mackenzie
R
R
Petersen
M M
MM
Beavis
M J
MJ
Williams
D
D
Thomas
N
N
Faict
D
D
Peluso
F
F
Coles
G A
GA
Davies
M
M
D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D002452:Cell Count / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015314:Dialysis Solutions / Q000592:standards*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D006801:Humans; D007975:Leukotriene B4 / Q000096:biosynthesis; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects; D010531:Peritoneal Dialysis, Continuous Ambulatory; D010587:Phagocytosis / Q000187:drug effects; D015054:Zymosan / Q000494:pharmacology
D015314:Dialysis Solutions; D007975:Leukotriene B4; D015054:Zymosan