Use of a non-extraction HPLC technique for measuring angiotensin-converting enzyme under optimum conditions.
A non-extraction high performance liquid chromatography assisted method for the measurement of angiotensin-converting enzyme in serum has been developed. Serum containing the enzyme was incubated with the synthetic substrate, furylacryloylphenylalanylglycylglycine at a concentration approaching 10 times the apparent Km, prior to injection onto a shielded hydrophobic reverse phase high performance liquid chromatography column. This allowed the product to be separated and measured directly without the need for a time consuming protein extraction step. The method is enzymatically sound and avoids the methodological problems associated with automated kinetic assays.
Badminton
M N
MN
Department of Chemical Pathology, Norfolk and Norwich Hospital, UK.
Dawson
C M
CM
Rainbow
S J
SJ
Tickner
T R
TR
D002712:Chlorides; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007700:Kinetics; D009842:Oligopeptides / Q000032:analysis / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000097:blood*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D002712:Chlorides; D009842:Oligopeptides; C038275:2-furanacryloyl-phenylalanyl-glycyl-glycine; D007703:Peptidyl-Dipeptidase A