title: Multiple IS insertion sequences near the replication terminus in Escherichia coli K-12.
abstract: In order to assess the feasibility of semi-automatic procedures for large genome sequencing, a fragment of 9.4 kb of Escherichia coli chromosomal DNA isolated at random was sequenced. It was found to map at 30 min on the chromosome map and to harbour two insertion sequences (IS2 and IS30) as well as several putative coding sequences which had no feature in common with known proteins.
authors.affiliation: Unité de régulation de l'expression génétique, Institut Pasteur, Paris, France.
authors.forename: I
authors.initials: I
authors.lastname: Moszer
authors.forename: P
authors.initials: P
authors.lastname: Glaser
authors.forename: A
authors.initials: A
authors.lastname: Danchin
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004261:DNA Replication; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D012371:Robotics; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial