The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM.
Infection of BALB/c mice with mouse hepatitis virus, strain JHM (MHV-JHM), at any of several intervals relative to ovalbumin (OVA) administration resulted in elevated OVA-specific IgG 2 a titers. Since gamma interferon (IFN) has been implicated as an up-regulator of IgG 2 a production, attempts were made to determine whether levels of this cytokine were modified in sera of infected mice. Serum IFN-gamma was not detected, but treatment of MHV-JHM-infected mice with monoclonal anti-IFN-gamma antibody resulted in high mortality with decreased survival times, enhanced virus titers in liver and spleen, and more severe virus-associated pathology, compared to mock-treated, infected mice. Immunotherapy with recombinant IFN-gamma ameliorated disease as reflected by mortality rates and virus titers in target organs.
Smith
A L
AL
Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut.
Barthold
S W
SW
de Souza
M S
MS
Bottomly
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D000914:Antibodies, Viral / Q000276:immunology; D004198:Disease Susceptibility; D005260:Female; D006524:Hepatitis, Viral, Animal / Q000276:immunology* / Q000382:microbiology / Q000628:therapy; D007371:Interferon-gamma / Q000276:immunology / Q000502:physiology* / Q000627:therapeutic use; D051379:Mice; D008807:Mice, Inbred BALB C; D006517:Murine hepatitis virus / Q000276:immunology* / Q000502:physiology; D010047:Ovalbumin / Q000008:administration & dosage; D011994:Recombinant Proteins; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D011994:Recombinant Proteins; D007371:Interferon-gamma; D010047:Ovalbumin