title: Simian sarcoma virus transformation of normal rat kidney fibroblasts is associated with markedly increased basic fibroblast growth factor expression.
abstract: Transformation of normal rat kidney fibroblasts (NRK) by the simian sarcoma virus (SSV) occurs as a result of expression of p28v-sis, a homologue of platelet-derived growth factor-B chain. Chromatographic separation revealed that the bulk (85%) of the mitogenic activity in SSV-transformed NRK cells was not due to p28v-sis but rather two distinct endothelial cell growth factors that eluted off heparin-Sepharose between 1 and 2 M NaCl. Protein purification and Northern blot analysis revealed that one of these growth factors was the 18 kd form of bFGF, the expression of which was found to increase 15-fold with SSV-transformation of NRK cells. The pure 18 Kd bFGF had no effect on NRK cell growth but was a potent neurotrophic agent for fetal rat cortical neurones and a potent growth factor for fetal bovine heart endothelial cells, suggesting a paracrine but not autocrine role for this protein. The second endothelial cell growth factor activity in SSV-transformed NRK cells was due to an 18 Kd protein which could be distinguished immunologically, biochemically, and mitogenically from bFGF.
authors.affiliation: Department of Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, MO 63110.
authors.forename: P G
authors.initials: PG
authors.lastname: Milner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002472:Cell Transformation, Viral; D002540:Cerebral Cortex / Q000187:drug effects; D002846:Chromatography, Affinity; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D005347:Fibroblasts / Q000382:microbiology; D015870:Gene Expression; D007668:Kidney / Q000382:microbiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009474:Neurons / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012842:Sarcoma Virus, Woolly Monkey / Q000235:genetics* / Q000378:metabolism
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2