title: Regional distribution of lipoxygenase activities in porcine ciliary epithelium.
abstract: Nonpigmented epithelial (NPE) cells and pigmented epithelial (PE) cells were separated from porcine ciliary processes without protease treatment. The relative capabilities of NPE cells and PE cells to synthesize 5-hydroxyeicosatetraenoic acid (5-HETE), 12-HETE, and 5,12-diHETE from 1-[14C] arachidonic acid were determined. HETE compounds were separated by HPLC. The capability of NPE cells to synthesize 5-lipoxygenase and 12-lipoxygenase products was, respectively, five-fold and 2.5 fold higher than that of PE cells. The formation of 5-HETE, 12-HETE and 5,12-diHETE was inhibited virtually completely by 50 microM nordihydroguaiaretic acid but not by 5 microM indomethacin. The major product formed both by NPE cells and PE cells was 12-HETE.
authors.affiliation: Kresge Eye Institute, Department of Ophthalmology, Wayne State University School of Medicine, Detroit, Michigan.
authors.forename: S
authors.initials: S
authors.lastname: Sakamoto
authors.forename: H
authors.initials: H
authors.lastname: Shichi
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism*; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D016718:Arachidonic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002924:Ciliary Body / Q000201:enzymology*; D004848:Epithelium / Q000201:enzymology; D006893:Hydroxyeicosatetraenoic Acids / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D007213:Indomethacin / Q000494:pharmacology; D007975:Leukotriene B4 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D009637:Masoprocol / Q000494:pharmacology; D010857:Pigment Epithelium of Eye / Q000201:enzymology*; D013552:Swine
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D006893:Hydroxyeicosatetraenoic Acids; D016859:Lipoxygenase Inhibitors; D007975:Leukotriene B4; D016718:Arachidonic Acid; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D009637:Masoprocol; D001092:Arachidonate 12-Lipoxygenase; D001094:Arachidonate 5-Lipoxygenase; D007213:Indomethacin