High level transient expression of the murine coronavirus haemagglutinin-esterase.
We have expressed the murine coronavirus haemagglutinin-esterase protein in a vaccinia virus/T7 RNA polymerase system. The levels of expression observed are significantly higher than those found in virus-infected cells. The expressed protein has both receptor-destroying (esterase) and receptor-binding (haemadsorption) activities. The use of this system will greatly facilitate analysis of the structure-function relationships of this protein.
Pfleiderer
M
M
Institute of Virology, University of Würzburg, Germany.
Routledge
E
E
Herrler
G
G
Siddell
S G
SG
D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004950:Esterases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics; D006367:HeLa Cells / Q000502:physiology; D006382:Hemadsorption; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D006517:Murine hepatitis virus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014616:Vaccinia virus / Q000235:genetics*; D014760:Viral Fusion Proteins; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C060814:hemagglutinin esterase; D004950:Esterases