Characterization and physiologic regulation of atrial natriuretic factor receptors in rabbit preglomerular renal microvessels.
There has been no direct demonstration of the presence of guanylate cyclase-linked atrial natriuretic factor receptors in renal preglomerular microvasculature. Using [125I]ANF, we have demonstrated the presence of high affinity (Kd = 80 pM) and low affinity (Kd = 7.2 nM) ANF receptors in membranes derived from rabbit renal preglomerular microvessels (afferent arterioles and interlobular arteries). These microvessels also exhibited the presence of particulate bound ANF-sensitive guanylate cyclase. The density of the high affinity ANF receptor in desoxycorticosterone-treated rabbits on a high-salt diet (31 +/- 3 fmol/mg protein) was nearly half of that seen in rabbits on a normal diet (53 +/- 4 fmol/mg protein; p less than 0.01, n = 4). Data from this study demonstrated the presence of renal preglomerular ANF receptors and suggested that these receptors (perhaps in addition to glomerular ANF receptors) may participate in the regulation of extracellular volume.
Chaudhari
A
A
Department of Veterans Affairs Medical Center, Long Beach, California.
Nakamura
S
S
Kirschenbaum
M A
MA
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001667:Binding, Competitive; D006152:Cyclic GMP / Q000032:analysis; D003900:Desoxycorticosterone / Q000494:pharmacology; D006162:Guanylate Cyclase / Q000032:analysis; D066298:In Vitro Techniques; D007678:Kidney Glomerulus / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay; D011869:Radioligand Assay; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*
D011956:Receptors, Cell Surface; D003900:Desoxycorticosterone; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP