title: Interaction of the Tn7-encoded transposition protein TnsB with the ends of the transposon.
abstract: We have used several high resolution methods to examine the interaction of TnsB, a transposition protein encoded by the bacterial transposon Tn7, with its binding sites at the ends of the transposon. These binding sites lie within the DNA segments that are directly involved in transposition. We show that the binding of TnsB to DNA can promote DNA bending, suggesting that the interaction of TnsB with the ends may result in formation of a highly organized protein-DNA complex. We also identify likely positions of close contact between of TnsB and its binding sites. Analysis of the interaction of TnsB with intact Tn7 ends reveals TnsB occupies its binding sites in a particular order, the sites immediately adjacent to the transposon termini being occupied only after other inner sites are bound. Such ordered occupancy suggests that the various binding sites have differing apparent affinities for TnsB.
authors.affiliation: Department of Microbiology and Immunology, University of California, San Francisco 94143.
authors.forename: L K
authors.initials: LK
authors.lastname: Arciszewska
authors.forename: N L
authors.initials: NL
authors.lastname: Craig
mesh_terms: D000478:Alkylation; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D029968:Escherichia coli Proteins; D006878:Hydroxides / Q000378:metabolism; D017665:Hydroxyl Radical; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D006878:Hydroxides; C069633:TnsB protein, E coli; D017665:Hydroxyl Radical; D003850:Deoxyribonuclease I