Immunohistochemical detection of EGF and NGF receptors in human olfactory epithelium.
The immunohistochemical localization of EGF and NGF receptors has been studied in the olfactory epithelium of human foetuses from 8 to 12 weeks of age. A positivity for EGF receptor, increasing with the age, was detected in the apical portion of the sensory epithelium. The NGF receptor was well detectable also at 8 weeks and localized both in differentiated olfactory cells and in some basal cells. From primary cultures of olfactory epithelium, a cell clone positive for Enolase, Neurofilaments and S-100 Protein was identified. These cells were shown to be reactive for EGF and NGF receptors. The addition of Retinoic acid to the culture medium induces a morphological differentiation of these cells that become positive for the Olfactory Marker Protein.
Balboni
G C
GC
Dipartimento di Anatomia umana e Istologia, Università di Firenze.
Zonefrati
R
R
Repice
F
F
Barni
T
T
Vannelli
G B
GB
D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D066246:ErbB Receptors / Q000032:analysis*; D005865:Gestational Age; D006801:Humans; D009419:Nerve Tissue Proteins / Q000032:analysis; D009475:Neurons, Afferent / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D009831:Olfactory Mucosa / Q000737:chemistry* / Q000166:cytology / Q000196:embryology; D011956:Receptors, Cell Surface / Q000032:analysis*; D017475:Receptors, Nerve Growth Factor; D014212:Tretinoin / Q000494:pharmacology
D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D014212:Tretinoin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors