A rapid method for the isolation of genomic DNA from citrated whole blood.
We have developed a DNA isolation method which allows the isolation of high quality DNA from frozen citrated blood and cell lines within 90 min. The procedure avoids the use of toxic organic reagents and tedious extraction steps. DNA prepared by this method is very well suited for restriction fragment length polymorphism and polymerase chain reaction analysis.
Parzer
S
S
Department of Medicine, University of Vienna, Austria.
Mannhalter
C
C
D001774:Blood Chemical Analysis; D001793:Blood Preservation; D015139:Blotting, Southern; D002951:Citrates; D019343:Citric Acid; D004247:DNA / Q000097:blood / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D002951:Citrates; D019343:Citric Acid; D004247:DNA