EPR study of the hydrophobic interaction of spectrin with fatty acids.
The hydrophobic interaction between spin-labelled stearic acid and spectrin was studied by electron paramagnetic resonance (EPR) and fluorescence quenching. The results are quantitatively interpreted in terms of two types of binding site on spectrin. A comparison between the results of the EPR and fluorescence experiments show the drawback of the fluorescence method in binding studies.
Streichman
S
S
Department of Hematology, Rambam Medical Center, Haifa, Israel.
Kahana
E
E
Silver
B L
BL
D001665:Binding Sites; D004578:Electron Spin Resonance Spectroscopy; D005453:Fluorescence; D013049:Spectrin / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism*; D014867:Water / Q000378:metabolism
D013229:Stearic Acids; D014867:Water; D013049:Spectrin; C031183:stearic acid