title: Ocular acyclovir delivery by collagen discs: a mouse model to screen anti-viral agents.
abstract: Discs 1.6 mm in diameter trephined from corneal collagen shields were used to deliver acyclovir (ACV) to the cornea of mice inoculated with herpes simplex virus type 1 (HSV-1). In the first minute after application to the cornea, there was a 23% decrease of ACV in the discs. After the first minute, ACV clearance from the discs appeared to be exponential with a half-life of 21 minutes. Treatment given 3 times a day reduced HSV-1 titer in tear film, corneal tissue, and trigeminal ganglia. This animal model should be useful to conserve novel potential anti-viral drugs undergoing initial screening.
authors.affiliation: Department of Neurology, City of Hope National Medical Center, Duarte, CA 91010.
authors.forename: D E
authors.initials: DE
authors.lastname: Willey
authors.forename: I
authors.initials: I
authors.lastname: Williams
authors.forename: C
authors.initials: C
authors.lastname: Faucett
authors.forename: H
authors.initials: H
authors.lastname: Openshaw
mesh_terms: D000212:Acyclovir / Q000493:pharmacokinetics*; D000818:Animals; D001684:Biological Dressings; D003094:Collagen; D003315:Cornea / Q000378:metabolism* / Q000382:microbiology; D004195:Disease Models, Animal; D004337:Drug Carriers; D004353:Drug Evaluation, Preclinical / Q000379:methods; D007635:Keratitis, Dendritic / Q000188:drug therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus
chemical_list: D004337:Drug Carriers; D003094:Collagen; D000212:Acyclovir