title: Effect of oral cholecalciferol supplementation at physiological and supraphysiological doses in naturally vitamin D3-deficient subterranean damara mole rats (Cryptomys damarensis).
abstract: The damara mole rat, Cryptomys damarensis, is a strictly subterranean dwelling herbivorous rodent that in its natural habitat has no access to any obvious source of cholecalciferol (D3). We examined the effects of D3 supplementation, at physiological and supraphysiological doses, on calcium metabolism, plasma concentrations of calcium and alkaline phosphatase (ALP) and D3 metabolites. Animals not receiving a D3 supplement maintained normal plasma calcium concentrations. In addition, they exhibited a high apparent fractional mineral absorption efficiency (91%) and maintained a positive mineral flux. The serum concentration of 25-(OH)D3 was undetectable (less than 5 nmol/l) and that of 1,25-(OH)2D3 was 41 +/- 10 pmol/l. Supplementation at a physiological dose of D3 resulted in increased plasma concentrations of D3 metabolites, food intake, apparent fractional absorption efficiency and apparent fractional retention efficiency. Despite the 1.8-fold increase in food intake, body mass remained constant suggesting that the enhanced energy intake was dissipated in catabolic processes. Plasma calcium and ALP concentrations were not significantly altered with physiological doses of D3. The group given supraphysiological doses of D3 exhibited hypercalcaemia, increased creatinine concentrations and markedly increased ALP levels. These data indicate that a pathological response to D3 intoxication occurred and that hepatic and renal excretory functions were impaired. It appears, therefore, that these animals function optimally at the low concentrations of D3 metabolites found naturally. Supplementation at both physiological and supraphysiological doses of D3 may disadvantage the damara mole rat.
authors.affiliation: Physiology Department, Medical School, University of the Witwatersrand, Parktown, South Africa.
authors.forename: R
authors.initials: R
authors.lastname: Buffenstein
authors.forename: D C
authors.initials: DC
authors.lastname: Skinner
authors.forename: S
authors.initials: S
authors.lastname: Yahav
authors.forename: G P
authors.initials: GP
authors.lastname: Moodley
authors.forename: M
authors.initials: M
authors.lastname: Cavaleros
authors.forename: D
authors.initials: D
authors.lastname: Zachen
authors.forename: F P
authors.initials: FP
authors.lastname: Ross
authors.forename: J M
authors.initials: JM
authors.lastname: Pettifor
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002112:Calcifediol / Q000097:blood; D002117:Calcitriol / Q000097:blood; D002118:Calcium / Q000378:metabolism; D002762:Cholecalciferol / Q000008:administration & dosage* / Q000378:metabolism; D002149:Energy Intake / Q000187:drug effects; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D012377:Rodentia / Q000378:metabolism*; D014808:Vitamin D Deficiency / Q000188:drug therapy*
chemical_list: D002762:Cholecalciferol; D000469:Alkaline Phosphatase; D002117:Calcitriol; D002112:Calcifediol; D002118:Calcium