title: Comparison of anti-RNA properties of normal and ellipticine functionalized alpha and beta-oligonucleotides.
abstract: RNA is not cleaved as a consequence of the binding of RNase H to the duplex between RNA and a complementary alpha-oligodeoxyribonucleotide (oligo). In consequence targets have been selected which do not a priori require the action of RNase H to inhibit genetic expression. Two models have been used: The Friend Murine Leukemia Virus (F-MuLV) and the synthesis of rabbit beta globin.alpha-oligos trigger specific inhibitions in both systems. The functionalisation in 5' with the intercalating agent 9-NH2-ellipticine renders the oligos resistant to degradation and allows a direct action on cells.
authors.affiliation: UA 147 CNRS, U 140 INSERM, Institut G. Roussy, Villejuif, France.
authors.forename: M
authors.initials: M
authors.lastname: Lavignon
authors.forename: J R
authors.initials: JR
authors.lastname: Bertrand
authors.forename: H
authors.initials: H
authors.lastname: Porumb
authors.forename: B
authors.initials: B
authors.lastname: Rayner
authors.forename: J L
authors.initials: JL
authors.lastname: Imbach
authors.forename: C
authors.initials: C
authors.lastname: Paoletti
authors.forename: C
authors.initials: C
authors.lastname: Malvy
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004611:Ellipticines / Q000494:pharmacology*; D005622:Friend murine leukemia virus / Q000187:drug effects / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D005914:Globins / Q000235:genetics; D007364:Intercalating Agents / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000378:metabolism / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects*; D012367:RNA, Viral / Q000187:drug effects*; D011817:Rabbits; D016914:Ribonuclease H / Q000378:metabolism*
chemical_list: D004611:Ellipticines; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D012367:RNA, Viral; C038373:9-aminoellipticine; D005914:Globins; D016914:Ribonuclease H