Inhibition of antibody producing cells (PFC) from Peyer patches by glycoproteins from Entamoeba histolytica.
It is well known that Entamoeba histolytica can cause damage to its host by means of enzymes, cytotoxins and perforins that affect different types of cells. In this work we investigated the effect of different Entamoeba histolytica trophozoites products over the secretion of antibodies by mice Peyer's patches using a reverse hemolytic assay. Results showed that amoebic glycoproteins inhibited the secretion of antibodies by lymphocytes from mice Peyer's patches. In all cases the decrease in antibody secretion was not due to cell death. The molecules responsible for this effect were shown to be high molecular weight immunogenic glycoproteins without enzymatic activity. The fact that amoebic glycoproteins interfere with plasmatic cells function could be another mechanism of evasion of the immune response by Entamoeba histolytica, although we still do not know how these amoebic products cause the inhibition.
Campos Rodríguez
R
R
Departamento de Bioquímica Aplicada, Escuela Superior de Medicina, IPN, México, D.F. México.
Castañeda Ibarra
F
F
García Montoya
R
R
Lara Padilla
E
E
García Latorre
E
E
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000921:Antibody-Producing Cells / Q000187:drug effects* / Q000276:immunology; D004748:Entamoeba histolytica / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006462:Hemolytic Plaque Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010581:Peyer's Patches / Q000166:cytology*; D015800:Protozoan Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D006023:Glycoproteins; D015800:Protozoan Proteins