Assignment of structures to oligosaccharides produced by enzymic degradation of a beta-D-glucan from barley by 1H- and 13C-n.m.r. spectroscopy.
The structures of one tri-(1), two tetra-(2 and 3), and one hexa-saccharide (4) produced by treatment of barley flour, after removal of the starch components, with a fungal beta-D-glucanase (Finizyme) have been assigned on the basis of 1H- and 13C-n.m.r. data as follows: beta-D-Glcp-(1----3)-beta-D-Glcp-(1----4)-D-Glcp (1), beta-D-Glcp-(1----4)-beta-D-Glcp-(1----3)-beta-D-Glcp-(1----4)-D-Glcp (2), beta-D-Glcp-(1----3)-beta-D-Glcp-(1----4)-beta-D-Glcp-(1----4)-D-Glcp (3), and beta-D-Xylp-(1----4)-[alpha-L-Araf-(1----3)]-[alpha-L-Ara f-(1----2)-beta-D-Xylp-(1----4)-beta-D-Xylp- (1----4)-D-Xylp (4).
Bock
K
K
Department of Chemistry, Carlsberg Laboratory, Gl. Carlsberg Vej 10. Valby, Denmark.
Duus
J O
JO
Norman
B
B
Pedersen
S
S
D002240:Carbohydrate Sequence; D005936:Glucans / Q000378:metabolism*; D001467:Hordeum / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011522:Protons; D013379:Substrate Specificity; D001617:beta-Glucosidase / Q000378:metabolism*
D005936:Glucans; D009844:Oligosaccharides; D011522:Protons; D001617:beta-Glucosidase