title: Specificity of new peptide immunoassays versus other immunoassays and agglutination tests for detection of HIV antibody in African sera.
abstract: The specificity of three HIV EIAs using synthetic peptides was evaluated and compared with that of four commercial EIAs using recombinant proteins or whole virus lysates of HIV-1 or of HIV-1 and HIV-2, and of two tests based on agglutination of sensitized particles. A total of 734 West African sera were screened. The best results were obtained with tests using recombinant proteins. HIV tests using synthetic peptides able to distinguish between HIV-1 and HIV-2 antibodies showed comparable specificity, but some false negative results represent a major handicap of these peptide EIAs in screening for HIV antibodies.
authors.affiliation: Département de Virologie, Centre Hospitalier Universitaire Dupuytren, Limoges, France.
authors.forename: G
authors.initials: G
authors.lastname: Léonard
authors.forename: M
authors.initials: M
authors.lastname: Verdier
authors.forename: A
authors.initials: A
authors.lastname: Sangaré
authors.forename: J L
authors.initials: JL
authors.lastname: Rey
authors.forename: F
authors.initials: F
authors.lastname: Denis
mesh_terms: D000372:Agglutination Tests / Q000379:methods*; D015153:Blotting, Western / Q000379:methods; D007560:Cote d'Ivoire; D005189:False Positive Reactions; D005260:Female; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology; D015498:HIV-2 / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007124:Immunoenzyme Techniques; D008297:Male; D010455:Peptides; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity
chemical_list: D015483:HIV Antibodies; D010455:Peptides; D011994:Recombinant Proteins