title: Extremely divergent histone H4 sequence from Trypanosoma cruzi: evolutionary implications.
abstract: Trypanosoma cruzi presents six histones electrophoretically resolved in three gel systems. Indirect evidence shows that one of these histones, named e, corresponds to H4 in other species. We present evidence that histone e is H4 by sequencing its amino terminal end. The amino terminal of T. cruzi histone H4, unlike that of other H4s examined thus far is not blocked. Moreover, this protein presents two variants. This partial amino acid sequence of T. cruzi histone H4 differs greatly from homologous sequences of human, yeast, or Tetrahymena. Since the conservatism of the core histones (H2A, H2B, H3, and H4) is clearly illustrated by comparative sequence analyses, the data shown here demonstrates that T. cruzi histone H4 is the most divergent reported. Quantitative analysis of the data suggests that the rate of substitutions in the histone H4 amino terminal sequence varies among different lineages. We postulate a slow-down in the evolutionary rate of histone H4 amino terminal domain in the metazoa branch related perhaps to the appearance of a novel function for this domain.
authors.affiliation: Department of Cell Biology and Genetics, School of Medicine, University of Chile, Santiago.
authors.forename: G C
authors.initials: GC
authors.lastname: Toro
authors.forename: C
authors.initials: C
authors.lastname: Wernstedt
authors.forename: C
authors.initials: C
authors.lastname: Medina
authors.forename: N
authors.initials: N
authors.lastname: Jaramillo
authors.forename: U
authors.initials: U
authors.lastname: Hellman
authors.forename: N
authors.initials: N
authors.lastname: Galanti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D014644:Genetic Variation; D006657:Histones / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000235:genetics*
chemical_list: D006657:Histones