title: Isolation and preliminary characterization of the Porphyromonas gingivalis prtC gene expressing collagenase activity.
abstract: A gene, prtC, has been isolated from Porphyromonas gingivalis ATCC 53977 in Escherichia coli utilizing the plasmid vector pPL-lambda. The resultant protease positive clone NHS1, harboring plasmid pS1 with a 5.9-kilobase P. gingivalis insert, expressed an enzyme capable of hydrolyzing the synthetic collagenase substrate PZ-PLGPA as well as solubilized type I collagen. Subcloning and deletion analysis located the prtC gene at one end of the P. gingivalis DNA insert on plasmid pS1.
authors.affiliation: Department of Pediatric Density, University of Texas Health Science Center, San Antonio 78284.
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: H K
authors.initials: HK
authors.lastname: Kuramitsu
mesh_terms: D015139:Blotting, Southern; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D003012:Microbial Collagenase / Q000235:genetics* / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D003012:Microbial Collagenase