Sequence analysis of the 4.7-kb BamHI-EcoRI fragment of the equine herpesvirus type-1 short unique region.
To localize gene that may encode immunogens potentially important for recombinant vaccine design, we have analysed a region of the equine herpesvirus type-1 (EHV-1) genome where a glycoprotein-encoding gene had previously been mapped. The 4707-bp BamHI-EcoRI fragment from the short unique region of the EHV-1 genome was sequenced. This sequence contains three entire open reading frames (ORFs), and portions of two more. ORF1 codes for 161 amino acids (aa), and represents the C terminus of a possible membrane-bound protein. ORF2 (424 aa) and ORF3 (550 aa) are potential glycoprotein-encoding genes; the predicted aa sequences contain possible signal sequences, N-linked glycosylation sites and transmembrane domains; they also show homology to the glycoproteins gI and gE of herpes simplex virus type-1 (HSV-1), and the related proteins of pseudorabies virus and varicella-zoster virus. The predicted aa sequence of ORF4 shares no homology with other known herpesvirus proteins, but the nucleotide sequence shows a high level of homology with the corresponding region of the EHV-4 genome. ORF5 may be related to US9 of HSV-1.
Elton
D M
DM
Department of Microbiology, University of Leeds, U.K.
Halliburton
I W
IW
Killington
R A
RA
Meredith
D M
DM
Bonass
W A
WA
D000595:Amino Acid Sequence; D001483:Base Sequence; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D006564:Herpesviridae / Q000235:genetics; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D011558:Herpesvirus 1, Suid / Q000235:genetics; D014645:Herpesvirus 3, Human / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI