Role of superoxide radicals in anoxia reoxygenation-mediated vascular contraction.
To determine the mechanism of anoxic vasoconstriction, precontracted rat aortic rings were exposed to 95% N2, which caused additional contraction. Reoxygenation (95% O2) resulted in initial relaxation followed by contraction. Indomethacin did not affect anoxic contraction or reoxygenation-mediated events, but NG-monomethyl-1-arginine, which inhibits EDRF synthesis, and oxyhemoglobin, which reduces EDRF activity, markedly decreased anoxic contraction and reoxygenation relaxation, and potentiated subsequent contraction. Superoxide dismutase did not affect anoxic contraction, but potentiated reoxygenation relaxation and attenuated subsequent contraction. Endothelin concentrations remained unchanged throughout anoxia and reoxygenation. Thus, anoxic contraction and reoxygenation-mediated early relaxation appear to be due to changes in EDRF. On the other hand, reoxygenation-induced contraction appears related to release of superoxide radicals.
Mehta
J L
JL
Division of Cardiology, University of Florida College of Medicine 32610.
Lawson
D L
DL
Yang
B C
BC
Haught
W H
WH
Hintze
T
T
D000818:Animals; D001013:Aorta, Thoracic; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D016232:Endothelins / Q000378:metabolism; D004730:Endothelium, Vascular / Q000502:physiology; D000860:Hypoxia; D007213:Indomethacin / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*; D010108:Oxyhemoglobins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D014661:Vasoconstriction / Q000187:drug effects; D019323:omega-N-Methylarginine
D016232:Endothelins; D010108:Oxyhemoglobins; D013481:Superoxides; D019323:omega-N-Methylarginine; D001120:Arginine; D013482:Superoxide Dismutase; D010100:Oxygen; D007213:Indomethacin