title: Comparison of staphylococcal co-agglutination with other assays for rapid diagnosis of rotavirus infection in humans, calves and piglets.
abstract: Co-agglutination was used for rapid diagnosis of rotavirus infection. Staphylococcus aureus Cowan I sensitized with rabbit antiserum to rotavirus demonstrated the presence of this virus in stools of children, calves and piglets with clinical signs of diarrhea. A total of 415 fecal samples were examined by the co-agglutination test and the results were compared with those of ELISA, electrophoresis in polyacrylamide gel and latex agglutination. The co-agglutination test, when compared to these three methods, presented a correlation which varied between 87.2 and 94.2%.
authors.affiliation: Laboratories of Virology, University of São Paulo, Brazil.
authors.forename: E L
authors.initials: EL
authors.lastname: Durigon
authors.forename: J A
authors.initials: JA
authors.lastname: Candeias
authors.forename: J A
authors.initials: JA
authors.lastname: Jerez
authors.forename: M J
authors.initials: MJ
authors.lastname: Bittencourt
authors.forename: E L
authors.initials: EL
authors.lastname: Ortolani
mesh_terms: D000256:Adenoviridae / Q000276:immunology; D000257:Adenoviridae Infections / Q000175:diagnosis; D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D002417:Cattle; D005243:Feces / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus; D013552:Swine