Interferon-gamma inhibition of interleukin 4-induced hyperproliferation of resting B cell from NZB/NZW F1 mice.
To characterize B cell hyperactivity in autoimmune NZB/NZW (B/W) F1 mice, we studied the effects of murine recombinant interferon gamma (IFN-gamma) on interleukin 4 (IL-4) induced resting B cell growth and differentiation. The number of resting B cells of B/W F1 mice were decreased, with more sensitivity to IL-4 than normal mice. Thus, resting B cell hyperresponsiveness to IL-4 was in a dose-dependent manner suppressed by IFN-gamma. This action was most noticeable when IFN-gamma was added to the culture system simultaneously with IL-4. As well, IFN-gamma did not exhibit cytotoxicity. These results suggest that IFN-gamma may have regulatory effects on IL-4 mediated B cell triggering.
Minoda
M
M
Third Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
Ohno
M
M
Hamada
K
K
Tomioka
Y
Y
Funauchi
M
M
Horiuchi
A
A
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis; D001327:Autoimmune Diseases / Q000235:genetics / Q000473:pathology*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D004247:DNA / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis; D007371:Interferon-gamma / Q000494:pharmacology*; D015847:Interleukin-4 / Q000037:antagonists & inhibitors*; D008070:Lipopolysaccharides; D008180:Lupus Erythematosus, Systemic / Q000235:genetics / Q000473:pathology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008814:Mice, Inbred NZB / Q000276:immunology*; D011994:Recombinant Proteins
D000974:Antibodies, Antinuclear; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D015847:Interleukin-4; D007371:Interferon-gamma; D004247:DNA