The effects of polysaccharides on plasminogen activation by single chain-and two chain-tissue plasminogen activator.
We examined the effects of polysaccharides on t-PA mediated plasminogen activation using single-chain tissue plasminogen activator (sct-PA) and two-chain tissue plasminogen activator (tct-PA). Unfractionated heparin, low molecular weight heparin (LMW heparin) and dextran sulfates enhanced the activation rate of plasminogen by sct-PA about three-fold to six-fold. Chondroitin sulfate C did not enhance the activation. The activation of plasminogen by tct-PA was slightly enhanced by unfractionated heparin, but not by other polysaccharides. Conversion of sct-PA to tct-PA was not stimulated by polysaccharides. SDS-PAGE showed no enhancement of the conversion from sct-PA to tct-PA by plasmin in the presence of polysaccharides. However, the enhancement of sct-PA mediated activation of plasminogen by unfractionated heparin, LMW heparin and dextran sulfates in the presence of aprotinin was shown with SDS-PAGE. It was suggested that unfractionated heparin, LMW heparin and dextran sulfates form complex with sct-PA and plasminogen, and stimulate the conversion of sct-PA to tct-PA.
Kawamura
H
H
Department of Ophthalmology Hamamatsu University School of Medicine Shizuoka, Japan.
Watanabe
I
I
Urano
T
T
Takada
Y
Y
Takada
A
A
D000818:Animals; D016264:Dextran Sulfate / Q000494:pharmacology; D005341:Fibrinolysin / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008968:Molecular Conformation; D010958:Plasminogen / Q000378:metabolism*; D011134:Polysaccharides / Q000494:pharmacology*; D010959:Tissue Plasminogen Activator / Q000737:chemistry / Q000494:pharmacology*
D006495:Heparin, Low-Molecular-Weight; D011134:Polysaccharides; D010958:Plasminogen; D006493:Heparin; D016264:Dextran Sulfate; D010959:Tissue Plasminogen Activator; D005341:Fibrinolysin