Phage abortive infection mechanism from Lactococcus lactis subsp. lactis, expression of which is mediated by an Iso-ISS1 element.
A 5-kb DNA fragment conferring a phage abortive infection phenotype (Abi+) has been cloned from Lactococcus lactis subsp. lactis IL416. The Abi+ determinant was subcloned on a 2-kb fragment which carried an Iso-ISS1 element and an open reading frame of 753 bp designated ORFX. Deletion within ORFX entailed the loss of the Abi+ phenotype, establishing that ORFX is the structural abi-416 gene. The expression of abi-416 was shown to be mediated by the Iso-ISS1 element, which contains a sequence fitting the consensus sequence for gram-positive promoters.
Cluzel
P J
PJ
Laboratoire de Génétique Microbienne, Institut de Biotechnologie, I.N.R.A., Jouy-en-Josas, France.
Chopin
A
A
Ehrlich
S D
SD
Chopin
M C
MC
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000502:physiology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007700:Kinetics; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000737:chemistry / Q000235:genetics; D016366:Open Reading Frames; D010641:Phenotype; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D019895:Transposases
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009713:Nucleotidyltransferases; D019895:Transposases