Dopamine D2-receptor mRNA level in rat striatum after chronic haloperidol treatment.
Chronic treatment with haloperidol, a D2-receptor antagonist and a neuroleptic, increases the number of D2-receptors in rat striatum. However, there have been inconsistent reports on the D2-receptor mRNA level, one showing the increase in the mRNA level and another detecting no changes. Furthermore, they did not distinguish the two isoforms of D2-receptor, D2A and D2B. In the present work, both D2- and DeA-receptor mRNA levels in rat striatum were estimated after chronic administration of haloperidol. There was, within the sensitivity of the assay, no significant increase in either of them between 3 and 24 h after the last administration. This suggests that chronic haloperidol treatment does not affect the transcriptional regulation of the D2-receptor gene or the alternative splicing process of its transcripts.
Matsunaga
T
T
Department of Psychiatry, Hamamatsu University School of Medicine, Japan.
Ohara
K
K
Natsukari
N
N
Fujita
M
M
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D006220:Haloperidol / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000096:biosynthesis*; D017448:Receptors, Dopamine D2; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D006220:Haloperidol