Peroxidase as a developmental marker in plant tissue culture.
Peroxidase was studied as a developmental marker in pumpkin (Cucurbita pepo L.) callus lines and horse-radish (Armoracia lapathifolia Gilib) transformants. Embryogenic callus lines DE grown on MS medium with 2.4-D and NA-3 grown on medium with NAA and adenine sulfate showed about a 20 times higher enzyme activity than the habituated non-embryogenic line Z5b/T grown on medium without hormones. A rise in peroxidase activity indicated that somatic embryogenesis was triggered in a few habituated tissue cultures. Separated globular embryoids had a manifold lower enzyme activity than the callus from which they originated. SDS-electrophoresis showed distinct polypeptide patterns between the horse-radish leaves and crown galls, but the tumor characteristic protein bands failed to be identified. In horse-radish crown galls and short bushy plants regenerated from hairy roots an enhanced peroxidase activity was registered. Due to its high peroxidase level and abundant biomass production horse-radish transformants should facilitate enzyme production.
Krsnik-Rasol
M
M
Department of Molecular Biology, Faculty of Science, University of Zagreb, Republic of Croatia, Yugoslavia.
D046508:Culture Techniques; D009195:Peroxidase / Q000032:analysis*; D063245:Plant Development; D010944:Plants / Q000648:ultrastructure; D010957:Plasmids; D014162:Transfection
D009195:Peroxidase