title: Synthetic DNA bending sequences increase the rate of in vitro transcription initiation at the Escherichia coli lac promoter.
abstract: Appropriately phased DNA bending sequences replacing the CAP binding site upstream from the lac promoter increase by roughly tenfold the rate of specific transcription initiation from a superhelical promoter template in vitro; promoter occlusion results from polymerase binding to the upstream (dA)n.(dT)n tracts, but this phenomenon is not responsible for the observed phase-dependent transcriptional activity. The rates of open complex formation at both P1 and P2 promoters respond in a similar phase-dependent way to the synthetic curved DNA sequences.
authors.affiliation: Department of Chemistry, Yale University, New Haven, CT 06511.
authors.forename: M R
authors.initials: MR
authors.lastname: Gartenberg
authors.forename: D M
authors.initials: DM
authors.lastname: Crothers
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D004278:DNA, Superhelical / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D007785:Lactose / Q000378:metabolism*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D002373:Cyclic AMP Receptor Protein; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D012321:DNA-Directed RNA Polymerases; D007785:Lactose