Degradation of galactomannan by a Clostridium butyricum strain.
An anaerobic, sporeforming, galactomannan-degrading bacterium was isolated from methanogenic granular sludge of an UASB reactor used for treatment of wastewater from a sugar refinery. The isolate degraded the polymer rapidly (doubling time was 55 min) and completely in mineral media. The bacterium was classified as Clostridium butyricum; the main products were acetate, butyrate, hydrogen, formate, and presumably CO2. The strain produced extracellular endo-mannanase, cell-associated exo-mannanase and intracellular alpha-galactosidase activity. The mannanases were present when grown on galactomannan, but not after growth on glucose, galactose, or mannose.
Dong
X Z
XZ
Department of Microbiology, Wageningen Agricultural University, The Netherlands.
Schyns
P J
PJ
Stams
A J
AJ
D001673:Biodegradation, Environmental; D050260:Carbohydrate Metabolism; D003013:Clostridium / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D005690:Galactose / Q000031:analogs & derivatives; D005947:Glucose / Q000378:metabolism*; D008351:Mannans / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008361:Mannosidases / Q000378:metabolism; D000519:alpha-Galactosidase / Q000378:metabolism; D044902:beta-Mannosidase
D003470:Culture Media; D008351:Mannans; C012990:galactomannan; D008361:Mannosidases; D000519:alpha-Galactosidase; D044902:beta-Mannosidase; D005947:Glucose; D008358:Mannose; D005690:Galactose