Protective effect of zinc-induced metallothionein synthesis on gentamicin nephrotoxicity in rats.
Wistar rats were used to study the protective effect of zinc-induced metallothionein (MT) synthesis on gentamicin nephrotoxicity. We found that s.c. pre-injection of ZnSO4 (Zn 10 mg/kg/day) for 5 days could ameliorate proximal tubular necrosis and acute renal failure caused by an 8-day s.c. injection of gentamicin (100 mg/kg/day), while preinjection of saline instead of zinc or zinc and gentamicin together could not. In the zinc-pretreated rats (n = 6), renal cortical metallothionein level was significantly higher than that of normal (n = 8, p less than 0.001) and the saline controls (n = 6, p less than 0.001). Since MT is a scavenger of hydroxyl radical, it is proposed that hydroxyl radical plays a role in the pathogenesis of gentamicin nephrotoxicity and that preinjection of zinc could ameliorate gentamicin nephrotoxicity via the induction of renal cortical MT synthesis.
Yang
C L
CL
Department of Nephrology, China-Japan Friendship Hospital.
Du
X H
XH
Zou
W Z
WZ
Chen
W
W
D058186:Acute Kidney Injury / Q000139:chemically induced*; D000818:Animals; D016166:Free Radical Scavengers; D005839:Gentamicins / Q000009:adverse effects*; D006878:Hydroxides; D017665:Hydroxyl Radical; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008668:Metallothionein / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology*; D019287:Zinc Sulfate
D016166:Free Radical Scavengers; D005839:Gentamicins; D006878:Hydroxides; D013431:Sulfates; D017665:Hydroxyl Radical; D008315:Malondialdehyde; D019287:Zinc Sulfate; D008668:Metallothionein; D013482:Superoxide Dismutase; D015032:Zinc