title: Regeneration of transgenic Lycium barbarum L.
abstract: A simple and effective system for the transformation and regeneration of Lycium barbarum L. has been developed. Young stem segments from Lycium barbarum L. were infected by Agrobacterium tumefaciens harboring a vector containing neomycin phosphotransferase II (npt-II) gene derived from non-oncogenic Ti plasmid. Calli originating from young stem segments on selective induction medium could differentiate into buds on selective differentiation medium rapidly and finally developed into whole plants. NPT-II enzyme activity assay and DNA hybridization indicated that the foreign gene had been integrated into the genome of Lycium barbarum L. and could be expressed in plants.
authors.affiliation: Institute of Genetics, Chinese Academy of Sciences, Beijing.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Huang
authors.forename: A
authors.initials: A
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Shao
authors.forename: D
authors.initials: D
authors.lastname: Niu
mesh_terms: D007612:Kanamycin / Q000494:pharmacology; D019868:Kanamycin Kinase; D007613:Kanamycin Resistance / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000033:anatomy & histology / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D010946:Plants, Medicinal / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic
chemical_list: D007612:Kanamycin; D010770:Phosphotransferases; D019868:Kanamycin Kinase