title: Cloning, sequence, and tissue distribution of a rabbit renal Na+/H+ exchanger transcript.
abstract: Rabbit kidney expresses a transcript that is similar to the human growth-factor-activatable Na+/H+ exchanger. PCR and library screening were used to clone overlapping 2.5 kb, 1.4 kb, and 1.8 kb cDNAs that together contain the entire coding region (2448 bp) and 5' untranslated region (726 bp) and part of the 3' untranslated region (128 bp) of a rabbit renal Na+/H+ exchanger transcript. The nucleotide and inferred amino acid sequences are highly conserved between rabbit and human (88% nucleotide identity, 95% amino acid identity). In rabbit, the transcript is expressed in both epithelial and non-epithelial tissues, with highest expression in stomach, brain, kidney, lung and ileum, and minimal expression in liver and skeletal muscle.
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510.
authors.forename: F
authors.initials: F
authors.lastname: Hildebrandt
authors.forename: J H
authors.initials: JH
authors.lastname: Pizzonia
authors.forename: R F
authors.initials: RF
authors.lastname: Reilly
authors.forename: N A
authors.initials: NA
authors.lastname: Rebouças
authors.forename: C
authors.initials: C
authors.lastname: Sardet
authors.forename: J
authors.initials: J
authors.lastname: Pouysségur
authors.forename: C W
authors.initials: CW
authors.lastname: Slayman
authors.forename: P S
authors.initials: PS
authors.lastname: Aronson
authors.forename: P
authors.initials: P
authors.lastname: Igarashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression / Q000235:genetics; D006859:Hydrogen / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid; D012964:Sodium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers
chemical_list: D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; D006859:Hydrogen; D012964:Sodium