title: Molecular characterization and peptide specificity of two vasoactive intestinal peptide (VIP) binding sites in the chicken pineal.
abstract: Here we report that chicken pineal membranes express high affinity receptors for Vasoactive Intestinal Peptide (VIP), as revealed by competitive displacement analysis of [3-iodotyrosyl-125I]-VIP by native VIP. These binding sites were further characterized by covalent cross-linking of radioiodinated VIP to chicken pineal cell membranes, using dithiobis(succinimidylpropionate) as a cross-linking reagent. Sodium dodecyl sulfate electrophoresis after solubilization of the cross-linked membranes, reveals the existence of two polypeptides, P1 and P2, with a similar labelling intensity and apparent molecular weights of Mr = 57,000 and Mr = 70,000 respectively. These two components behave like high affinity binding sites for VIP.
authors.affiliation: Laboratoire de Biologie Moléculaire (URA 1172, CNRS) Université de Poitiers, France.
authors.forename: A C
authors.initials: AC
authors.lastname: Meunier
authors.forename: P
authors.initials: P
authors.lastname: Voisin
authors.forename: G
authors.initials: G
authors.lastname: Van Camp
authors.forename: Y
authors.initials: Y
authors.lastname: Cenatiempo
authors.forename: J M
authors.initials: JM
authors.lastname: Muller
mesh_terms: D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens; D003432:Cross-Linking Reagents; D000242:Cyclic AMP / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D010870:Pineal Gland / Q000737:chemistry*; D011869:Radioligand Assay; D011964:Receptors, Gastrointestinal Hormone / Q000737:chemistry*; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D007457:Iodine Radioisotopes; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP; D001191:Arylamine N-Acetyltransferase