title: Charge determination of product ions formed from collision-induced dissociation of multiply protonated molecules via ion/molecule reactions.
abstract: The use of ion/molecule reactions involving multiply protonated ions derived from electrospray for the determination of the charges of product ions formed from collision-induced dissociation is described. The experiments are carried out with a quadrupole ion trap capable of multiple stages of mass spectrometry. The approach is illustrated with proton transfer from a product ion from quadruply protonated melittin, and from a product ion from the (M + 20H)20+ ion from horse myoglobin, to 1,6-diaminohexane. The major product ion from quadruply protonated bovine insulin is used to illustrate the use of a clustering reaction with 1,6-diaminohexane. The ion trap is shown to be a particularly useful tool for employing both collisional activation and low-energy ion/molecule reactions in the same experiment to determine product ion charge.
authors.affiliation: Analytical Chemistry Division, Oak Ridge National Laboratory, Tennessee 37831-6365.
authors.forename: S A
authors.initials: SA
authors.lastname: McLuckey
authors.forename: G L
authors.initials: GL
authors.lastname: Glish
authors.forename: G J
authors.initials: GJ
authors.lastname: Van Berkel
mesh_terms: D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006736:Horses; D007328:Insulin / Q000032:analysis; D008555:Melitten / Q000032:analysis; D009132:Muscles / Q000737:chemistry; D009211:Myoglobin / Q000032:analysis; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D011522:Protons
chemical_list: D007328:Insulin; D009211:Myoglobin; D011506:Proteins; D011522:Protons; D008555:Melitten