title: A hybrid form of alkaline phosphatase produced in FL-amnion cells.
abstract: FL-amnion cells have mainly two alkaline phosphatase (AP) isozymes, of which the faster migrating one (FL-APF) on 5% polyacrylamide gel electrophoresis has proved to be identical to the Kasahara isozyme, a tumor-associated AP of intestinal type. But the other slower migrating one (FL-APs) remains to be characterized. Immunological and enzymic examination of purified FL-APs revealed that it is a hybrid form of AP consisting of one subunit with a molecular weight of 66,200 coming from the same subunit as that of FL-APF and another one with a molecular weight of 58,000 from placental AP subunit with modified glycosylation.
authors.affiliation: Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hada
authors.forename: H
authors.initials: H
authors.lastname: Imanishi
authors.forename: K
authors.initials: K
authors.lastname: Muratani
authors.forename: K
authors.initials: K
authors.lastname: Hirano
authors.forename: K
authors.initials: K
authors.lastname: Higashino
mesh_terms: D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D000650:Amnion / Q000201:enzymology*; D006528:Carcinoma, Hepatocellular / Q000201:enzymology; D002460:Cell Line; D006801:Humans; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000302:isolation & purification*; D008113:Liver Neoplasms / Q000201:enzymology; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D000469:Alkaline Phosphatase