title: Multiple forms of statherin in human salivary secretions.
abstract: Sequential chromatography of hydroxyapatite-adsorbed salivary proteins from submandibular/sublingual secretions on Sephadex G-50 and reversed-phase HPLC resulted in the purification of statherin and several statherin variants. Amino acid analysis, Edman degradation and carboxypeptidase digestion of the obtained protein fractions led to the determination of the complete primary structures of statherin SV1, statherin SV2, and statherin SV3. SV1 is identical to statherin but lacks the carboxyl-terminal phenylalanine residue. SV2, lacking residues 6-15, is otherwise identical to statherin. SV3 is identical to SV2 but lacks the carboxyl-terminal phenylalanine. These results provide the first evidence for multiple forms of statherin which are probably derived both by post-translational modification and alternative splicing of the statherin gene.
authors.affiliation: Department of Periodontology and Oral Biology, Boston University School of Graduate Dentistry, MA.
authors.forename: J L
authors.initials: JL
authors.lastname: Jensen
authors.forename: M S
authors.initials: MS
authors.lastname: Lamkin
authors.forename: R F
authors.initials: RF
authors.lastname: Troxler
authors.forename: F G
authors.initials: FG
authors.lastname: Oppenheim
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D017886:Durapatite; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry; D008969:Molecular Sequence Data; D010649:Phenylalanine / Q000032:analysis; D010768:Phosphoserine / Q000032:analysis; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014443:Tyrosine / Q000032:analysis
chemical_list: D000596:Amino Acids; D006882:Hydroxyapatites; C018149:STATH protein, human; D012471:Salivary Proteins and Peptides; D010768:Phosphoserine; D014443:Tyrosine; D010649:Phenylalanine; D017886:Durapatite