title: The effect of atrial natriuretic peptide on intestinal electrolyte transport.
abstract: The effect of atrial natriuretic peptide (ANP) on rat small intestinal electrolyte transport was examined. In vivo, intravenous administration of rat ANP(99-126) induced diuresis and natriuresis in conjunction with a significant decrease in intestinal water (basal, 37.1 +/- 5.7 versus ANP 28.5 +/- 6.0 microliters/cm per 20 min, P less than 0.05) and Na+ (4.0 +/- 0.7 versus 2.8 +/- 0.9 mumol/cm per 20 min, P less than 0.05) absorption (n = 9). In vitro, in Ussing chambers, in both jejunum and ileum, addition of 1.0 microM ANP to short circuited, stripped tissue produced a maximal increase in short circuit current and stimulated net Cl- secretion due to a significant increase in the unidirectional serosal to mucosal flux (JCl-sm: jejunum 17.4 +/- 1.3 versus 19.8 +/- 1.3 microEq/cm2 per h, P less than 0.01, n = 6; ileum 13.4 +/- 0.5 versus 17.2 +/- 0.6, P less than 0.01, n = 6) which was inhibited by the calcium channel antagonist verapamil (82 +/- 26%, P less than 0.05) and by the 5-HT2 receptor antagonist cinanserin (72 +/- 44%, P less than 0.05). Guanylate cyclase activity was stimulated by ANP in intact epithelium, but not in isolated crypt and villus enterocytes.
authors.affiliation: Intestinal Disease Research Unit, University of Calgary, Alberta, Canada.
authors.forename: A G
authors.initials: AG
authors.lastname: Catto-Smith
authors.forename: J A
authors.initials: JA
authors.lastname: Hardin
authors.forename: M K
authors.initials: MK
authors.lastname: Patrick
authors.forename: E V
authors.initials: EV
authors.lastname: O'Loughlin
authors.forename: D G
authors.initials: DG
authors.lastname: Gall
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004573:Electrolytes / Q000378:metabolism*; D005260:Female; D006632:Histamine / Q000378:metabolism; D006633:Histamine Antagonists; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology; D014867:Water / Q000378:metabolism
chemical_list: D002712:Chlorides; D004573:Electrolytes; D006633:Histamine Antagonists; D010446:Peptide Fragments; D014867:Water; D006632:Histamine; D009320:Atrial Natriuretic Factor; D012964:Sodium; D014700:Verapamil; D006152:Cyclic GMP; D011188:Potassium; D002118:Calcium