Luminol chemiluminescence and active oxygen generation by activated neutrophils.
Upon stimulation by various ligands and membrane perturbers, neutrophils produce various active oxygen species. Since luminol chemiluminescence (LCL) in neutrophils can be blocked by azide, an inhibitor of myeloperoxidase, LCL has been believed to reflect mainly the myeloperoxidase-catalyzed reaction. When cells were stimulated by formyl-methionyl-leucyl-phenylalanine, LCL was strongly inhibited by superoxide dismutase (SOD) and uric acid, a scavenger for hydroxy radical (.OH) and singlet oxygen, whereas it was stimulated by azide. LCL was also inhibited by .OH scavengers, such as mannitol, ethanol, and dimethylsulfoxide. However, when stimulated by phorbol myristate acetate or opsonized zymosan, LCL was strongly inhibited by azide but not by uric acid, and the inhibitory action of SOD was low. Thus, the qualitative and quantitative aspects of reactive oxygen generation by activated neutrophils differ significantly from one ligand to another. These results suggest that the metabolic fate of active oxygens in neutrophils and, hence, their effect on microorganisms and the surrounding tissues might differ depending on the stimulus.
Takahashi
R
R
Department of Medical Biology, Kochi Medical School, Japan.
Edashige
K
K
Sato
E F
EF
Inoue
M
M
Matsuno
T
T
Utsumi
K
K
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology; D016166:Free Radical Scavengers; D006168:Guinea Pigs; D006878:Hydroxides; D017665:Hydroxyl Radical; D008163:Luminescent Measurements; D008165:Luminol; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D009895:Opsonin Proteins; D010100:Oxygen / Q000378:metabolism*; D009195:Peroxidase / Q000037:antagonists & inhibitors; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014527:Uric Acid / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D001095:Arachidonic Acids; D001386:Azides; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D006878:Hydroxides; D009895:Opsonin Proteins; D013481:Superoxides; D014527:Uric Acid; D016718:Arachidonic Acid; D017665:Hydroxyl Radical; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008165:Luminol; D015054:Zymosan; D009195:Peroxidase; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen