title: The application of AmpliProbe in diagnostics.
abstract: AmpliProbe System is a rapid, enzyme-labeled, non-isotopic probe system that has high sensitivity and flexibility. AmpliProbe System consists of two major components: target-specific "primary" probes and target-independent, enzyme-labeled, signal-generating "secondary" probes. The visualization of the complemental hybridization between the target DNA or RNA and probes is accomplished by an enzymatic chemiluminescent reaction. The AmpliProbe System format allows hybridization and signal visualization to be completed within five to seven hours. In this paper we present several successful applications of AmpliProbe in the detection of infectious disease pathogens and the detection of gene amplification and transcription elevation in the evaluation of oncogenes in cancer research.
authors.affiliation: ImClone Systems, Inc., New York, NY 10014.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Yang
authors.forename: P V
authors.initials: PV
authors.lastname: Tata
authors.forename: H S
authors.initials: HS
authors.lastname: Park-Turkel
authors.forename: H W
authors.initials: HW
authors.lastname: Waksal
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D002478:Cells, Cultured; D015342:DNA Probes; D004279:DNA, Viral / Q000302:isolation & purification; D005784:Gene Amplification; D006509:Hepatitis B / Q000175:diagnosis* / Q000382:microbiology; D006515:Hepatitis B virus / Q000302:isolation & purification*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics*; D009857:Oncogenes
chemical_list: D015342:DNA Probes; D004279:DNA, Viral