title: Chemical structure of the carbohydrate backbone of Vibrio parahaemolyticus serotype 012 lipopolysaccharide.
abstract: The chemical structure of the saccharide portion of Vibrio parahaemolyticus serotype 012 lipopolysaccharide was studied. Using chemical degradation and modification, as well as methylation analysis in combination with GLC-MS, laser-desorption mass spectrometry and 1H-NMR and 13C-NMR spectroscopy, the carbohydrate backbone of the lipopolysaccharide was characterized as a branched decasaccharide with the following structure: (formula; see text) In the native lipopolysaccharide two additional phosphate groups are present and 3-deoxy-D-threo-hexulosonic acid and D-galacturonic acid are bound via acid-labile linkages.
authors.affiliation: Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kondo
authors.forename: U
authors.initials: U
authors.lastname: Zähringer
authors.forename: U
authors.initials: U
authors.lastname: Seydel
authors.forename: V
authors.initials: V
authors.lastname: Sinnwell
authors.forename: K
authors.initials: K
authors.lastname: Hisatsune
authors.forename: E T
authors.initials: ET
authors.lastname: Rietschel
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002857:Chromium; D017608:Chromium Compounds; D008401:Gas Chromatography-Mass Spectrometry; D006603:Hexuronic Acids / Q000032:analysis; D007834:Lasers; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010504:Periodic Acid; D010710:Phosphates / Q000032:analysis; D013400:Sugar Acids / Q000032:analysis; D014736:Vibrio parahaemolyticus / Q000737:chemistry*
chemical_list: D017608:Chromium Compounds; D006603:Hexuronic Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D010710:Phosphates; D013400:Sugar Acids; D002857:Chromium; D010504:Periodic Acid; C002532:2-keto-3-deoxyoctonate; C007819:galacturonic acid; C028801:chromium trioxide