Characterization of rhesus macaque B-lymphoblastoid cell lines infected with simian type D retrovirus.
A simian type D retrovirus designated SRV induces a fatal immunosuppressive disease in rhesus macaques. This syndrome shows many clinical similarities to acquired immunodeficiency syndrome (AIDS) in human immunodeficiency virus-infected individuals. To investigate the mechanisms of immune dysfunction in SRV infection, we have focused on the interactions of SRV serotype 1 (SRV-1) with macaque B-lymphoblastoid cell lines (B-LCL). Procedures were optimized for establishing B-LCL by immortalization of macaque B lymphocytes with rhesus Epstein-Barr virus (EBV). These cell lines express B-cell surface markers, secrete immunoglobulins of the IgG or IgM isotypes, and release EBV which transforms monkey B cells. In vitro cultures of B-LCL supported replication of SRV-1. Several B-LCL infected with SRV-1 showed downregulation of major histocompatibility complex (MHC) class II antigen expression whereas levels of MHC class I antigen remained unchanged. Infection of B-LCL with SRV-1 did not alter the level of secreted immunoglobulin. Rhesus EBV was also used to obtain B-LCL from macaques infected with SRV-1; these cell lines were found to release infectious SRV-1. Investigations on the interactions of SRV-1 with B cells will be useful for elucidating mechanisms involved in the immunopathogenesis of primate retroviruses.
van Kuyk
R W
RW
Department of Medical Pathology, University of California, Davis 95616.
Acevedo
R A
RA
Torres
J V
JV
Levy
N B
NB
Planelles
V
V
Munn
R J
RJ
Unger
R E
RE
Gardner
M B
MB
Luciw
P A
PA
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology / Q000648:ultrastructure; D001483:Base Sequence; D002461:Cell Line, Transformed; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D010641:Phenotype; D015301:Retroviruses, Simian / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000473:pathology
D000954:Antigens, Surface