Protective effect of chromium(III) on acute lethal toxicity of carbon tetrachloride in rats and mice.
Trivalent chromium (Cr(III)) preadministered intraperitoneally (5 mg Cr/kg body weight) to rats and mice protected these animals from acute lethal toxicity of carbon tetrachloride (CCl4). Some other metals, Cr(VI), Cu(II), and Zn(II), had no effect on CCl4 lethal toxicity. DL-alpha-tocopherol, one of the antioxidative agents, showed similar protective effects to Cr(III). Activities of serum GOT and GPT in mice were increased sharply by the administration of CCl4, but these elevations were depressed by Cr(III) preadministration. Serum glucose levels of mice increased transiently after CCl4 administration and then in the control group fell to hypoglycemic levels after 6 hr, whereas the Cr(III)-pretreated group kept to homeostatic levels. Lipid peroxidation of microsomes in mice 24 hr after Cr(III) administration was lower than that of the control. These results suggest that Cr(III) preadministered to mice might act as a radical scavenger to CCl4 to form trichloromethyl radicals which are a major initial product of CCl4 in liver cells.
Tezuka
M
M
Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Momiyama
K
K
Edano
T
T
Okada
S
S
D000818:Animals; D002251:Carbon Tetrachloride / Q000037:antagonists & inhibitors* / Q000633:toxicity; D002712:Chlorides; D002857:Chromium / Q000627:therapeutic use*; D017608:Chromium Compounds; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D002712:Chlorides; D017608:Chromium Compounds; D002857:Chromium; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride; D005952:Glucose-6-Phosphatase; C022990:chromic chloride