title: IS801, an insertion sequence element isolated from Pseudomonas syringae pathovar phaseolicola.
abstract: A transposable element, designated IS801, was isolated from strain LR781 of Pseudomonas syringae pathovar phaseolicola in two independent events using the entrapment plasmid, pUCD800. IS801 is 1517 base pairs in length and contains open reading frames that potentially encode proteins of 311 and 172 amino acids, as well as smaller proteins. Unlike most other prokaryotic transposable elements, IS801 lacks terminal repeats. Sequence analysis revealed two target pentamers for IS801 insertion that differ by one base pair. One copy of IS801 generated a perfect duplication of its target, TGAAC. The second copy of IS801 was flanked by the target, TGGAC, at one end, and TGAAC at the other end. A third copy of IS801 was cloned from pMMC7105, an indigenous plasmid of strain LR781, and it was flanked by copies of the pentamer TGAAC.
authors.affiliation: Department of General Microbiology, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Romantschuk
authors.forename: G Y
authors.initials: GY
authors.lastname: Richter
authors.forename: P
authors.initials: P
authors.lastname: Mukhopadhyay
authors.forename: D
authors.initials: D
authors.lastname: Mills
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011549:Pseudomonas / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements