Herpes simplex virus type 1 that exhibits herpes simplex virus type 2 sensitivity to (E)-5-(2-bromovinyl)-2'-deoxyuridine.
A clinical isolate, designated 145, of herpes simplex virus (HSV) had type 1 characteristics as determined by monoclonal antibody immunofluorescence, heat stability of viral thymidine kinase (TK), BamHI restriction endonuclease pattern, and absence of the HSV-2-specific 38-kD protein. However, instead of being sensitive to E-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) like HSV-1, isolate 145 displayed a resistance pattern like HSV-2 to the drug as determined by viral replication and viral DNA synthesis. Because BVDU is activated by viral TK phosphorylation, we cloned the TK-containing DNA region from isolate 145 and compared it by restriction mapping using several endonucleases to similar regions of HSV-1 and HSV-2. In each instance, the patterns for HSV-1 and isolate 145 were identical to each other, but distinct from the patterns for the corresponding region of HSV-2, suggesting that the genome TK region of isolate 145 was HSV-1-like.
Docherty
J J
JJ
Department of Microbiology/Immunology, Northeastern Ohio Universities College of Medicine, Rootstown 44272.
Dobson
A T
AT
Trimble
J J
JJ
Jennings
B A
BA
D000818:Animals; D000911:Antibodies, Monoclonal; D000998:Antiviral Agents / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D004352:Drug Resistance, Microbial; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D018139:Simplexvirus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000032:analysis / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects
D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D004279:DNA, Viral; C020235:brivudine; D013937:Thymidine Kinase; D001973:Bromodeoxyuridine