Nonisotopic receptor-binding assay for benzodiazepine receptors utilizing a fluorophore labeled ligand.
A nonisotopic receptor-binding assay method provides a new approach for the study of receptor-ligand interactions and a possible receptor assay for benzodiazepine drugs. The proposed method is based upon the use of fluorescence-labeled drugs and a chromatographic system which accepts samples without deproteinization. The effectiveness of the technique is illustrated in a study of benzodiazepine receptor-drug-binding interactions.
Takeuchi
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Hawaii Biosensor Laboratory, Department of Chemistry, University of Hawaii at Manoa, Honolulu 96822.
Rechnitz
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D000345:Affinity Labels; D000818:Animals; D001569:Benzodiazepines / Q000378:metabolism*; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D003374:Coumarins / Q000378:metabolism*; D005456:Fluorescent Dyes; D005479:Flurazepam / Q000031:analogs & derivatives* / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008722:Methods; D011963:Receptors, GABA-A / Q000032:analysis* / Q000378:metabolism; D013057:Spectrum Analysis
D000345:Affinity Labels; D003374:Coumarins; D005456:Fluorescent Dyes; D008024:Ligands; D011963:Receptors, GABA-A; D001569:Benzodiazepines; C070091:Ro7-AMCA; D005479:Flurazepam