title: Identification of an alpha 2-adrenoceptor in human coronary arteries by radioligand binding assay.
abstract: A single high affinity binding site for an alpha 2-adrenoceptor in human coronary arteries was identified by radioligand binding assay. Human coronary arteries were obtained at autopsy within 6 hours of death. A crude membrane solution was incubated with (3H)-rauwolscine at 25 degrees C for 30 min. The binding of (3H)-rauwolscine was rapidly saturable and reversible. Kd was 1.2 +/- 0.2 (SE) nM and Bmax 22 +/- 3 fmol/mg protein. This is the first study which has shown the presence of an alpha 2-adrenoceptor in human coronary arteries using a radioligand binding assay method.
authors.affiliation: Second Department of Internal Medicine, Yokohama City University, School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: S
authors.initials: S
authors.lastname: Umemura
authors.forename: K
authors.initials: K
authors.lastname: Uchino
authors.forename: T
authors.initials: T
authors.lastname: Shindou
authors.forename: G
authors.initials: G
authors.lastname: Yasuda
authors.forename: K
authors.initials: K
authors.lastname: Minamisawa
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: N
authors.initials: N
authors.lastname: Hirawa
authors.forename: M
authors.initials: M
authors.lastname: Ishii
mesh_terms: D001158:Arteries / Q000378:metabolism*; D001667:Binding, Competitive; D003331:Coronary Vessels / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D011869:Radioligand Assay; D011942:Receptors, Adrenergic, alpha / Q000737:chemistry*; D015016:Yohimbine / Q000378:metabolism
chemical_list: D011942:Receptors, Adrenergic, alpha; D015016:Yohimbine