[The source of the iron binding with nitric oxide in activated macrophages].
No decrease in iron-sulphur centers was found in cultured macrophage cells (J774) after the treatment with nitric oxide (10(-7) M NO/10(7) cells) during 5 min. The center content was controlled by the electron spin resonance (ESR) method. The macrophages pretreated with dithionite + methyl viologen showed the formation of dinitrosyl iron complexes (DNIC) with a characteristic ESR signal at g approximately 2.03. The data suggest that loosely bound nonheme iron (free iron) mostly contributes to the formation of these complexes. Iron from iron-containing proteins does not release from these centers under the direct action of nitric oxide. The iron-sulphur centers can be destroyed by the products of nitric oxide oxidation (NO2, N2O3, etc.) as oxidizing and acid agents.
Vanin
A F
AF
Men'shikov
G B
GB
Moroz
I A
IA
Mordvintsev
P I
PI
Serezhenkov
V A
VA
Repin
V S
VS
Burbaev
D Sh
DSh
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D007501:Iron / Q000032:analysis / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000032:analysis / Q000378:metabolism; D008262:Macrophage Activation / Q000502:physiology*; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*
D007506:Iron-Sulfur Proteins; D009569:Nitric Oxide; D007501:Iron
Ob istochnike zheleza, sviazyvaiushchegosia s oksidom azota v aktivirovannykh makrofagakh.