Synthesis of insulin-dependent activator of hepatocyte plasmatic membrane Na,K-ATPase decreases in aging.
The experiments on Wistar rats of different age-groups have shown that insulin (1.6 units/kg) provokes hyperpolarization of hepatocyte plasmatic membranes in adult rats, but not in old ones. The hyperpolarization can be prevented by actinomycin D and ouabain, while 2-aminopyridine is ineffective in this case. The appearance of hyperpolarization correlates with the activation of Na,K-ATPase in hepatocyte plasmatic membrane. It is shown that insulin injection provokes the appearance of Na,K-ATPase activation factor in the hepatocyte cytosol. The synthesis of this factor reduces with age.
Frolkis
V V
VV
Institute of Gerontology AMS USSR, Kiev.
Shkapenko
A L
AL
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D003600:Cytosol / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008564:Membrane Potentials / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D007328:Insulin; D000254:Sodium-Potassium-Exchanging ATPase