title: Role of interleukin-6 in the neuroendocrine system.
abstract: Interleukin-6 (IL-6) is a pleiotropic cytokine exerting many immunological and non immunological actions. The cytokine binds to a specific receptor, whose activation induces the association with a novel transducer, the glycoprotein gp 130. Here we present our results about the effect of IL-6 on both hormone secretion and second messenger systems at pituitary level, and the production of IL-6 from cells of central nervous system. IL-6 inhibited basal, VIP and TRH-stimulated prolactin (PRL) secretion from single lactotropes, studied by means of reverse hemolytic plaque assay, whereas in primary cultures of anterior pituitary cells, according to the literature, the cytokine stimulated prolactin secretion. IL-6 did not affect basal adenylate cyclase activity, inositol phosphate production, and cytosolic calcium concentration. Conversely, the preincubation of pituitary cells with interleukin-6 for 20 min significantly reduced VIP- and forskolin-stimulated adenylate cyclase activity, as well as inositol phosphate production and free cytosolic calcium increase induced by TRH.
authors.affiliation: Dept. of Communicative Science, 2. School of Medicine, University of Naples.
authors.forename: G
authors.initials: G
authors.lastname: Schettini
authors.forename: M
authors.initials: M
authors.lastname: Grimaldi
authors.forename: E
authors.initials: E
authors.lastname: Landolfi
authors.forename: O
authors.initials: O
authors.lastname: Meucci
authors.forename: C
authors.initials: C
authors.lastname: Ventra
authors.forename: T
authors.initials: T
authors.lastname: Florio
authors.forename: A
authors.initials: A
authors.lastname: Scorziello
authors.forename: A
authors.initials: A
authors.lastname: Marino
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects / Q000502:physiology; D007239:Infections / Q000503:physiopathology; D007249:Inflammation / Q000503:physiopathology; D015850:Interleukin-6 / Q000096:biosynthesis / Q000494:pharmacology / Q000502:physiology*; D009490:Neurosecretory Systems / Q000502:physiology*; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D010908:Pituitary Hormones, Anterior / Q000378:metabolism; D010913:Pituitary-Adrenal System / Q000502:physiology; D051381:Rats; D015398:Signal Transduction
chemical_list: D015850:Interleukin-6; D010908:Pituitary Hormones, Anterior; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone