title: An efficient technique for obtaining sequences flanking inserted retroviruses.
abstract: Genomic mapping studies frequently employ retrovirus-mediated transfer of dominant selectable markers to specific target chromosomes. DNA probes containing sequences adjacent to inserted proviruses are valuable mapping tools in such studies. We have implemented a strategy for amplification of chromosomal sequences flanking the 5' LTR of MoMuLV-based vectors. Probes derived from these amplification products successfully differentiated murine versus human proviral localization in retrovirus-infected mouse-human chromosome 17q hybrid cells.
authors.affiliation: Genmap Inc., New Haven, CT 06511.
authors.forename: C S
authors.initials: CS
authors.lastname: Huckaby
authors.forename: R E
authors.initials: RE
authors.lastname: Kouri
authors.forename: M J
authors.initials: MJ
authors.lastname: Lane
authors.forename: S M
authors.initials: SM
authors.lastname: Peshick
authors.forename: W T
authors.initials: WT
authors.lastname: Carroll
authors.forename: S M
authors.initials: SM
authors.lastname: Henderson
authors.forename: B D
authors.initials: BD
authors.lastname: Faldasz
authors.forename: P G
authors.initials: PG
authors.lastname: Waterbury
authors.forename: J N
authors.initials: JN
authors.lastname: Vournakis
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D015342:DNA Probes; D004279:DNA, Viral / Q000302:isolation & purification; D008040:Genetic Linkage; D005822:Genetic Vectors; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D004247:DNA