title: Suitability of polyethylene glycol for precipitation of monoclonal antibodies--a radiometric study.
abstract: 125I labelled monoclonal antibody (MoAb) to thyroid stimulating hormone (TSH) was used as a tracer for assessing the suitability of polyethylene glycol (PEG) for precipitation of MoAb and MoAb-antigen (AG) complexes. It was found that PEG can be advantageously used under certain conditions favouring insolubility and could thus be a non-specific separation reagent for radioimmunoassays based on MoAbs.
authors.affiliation: Radiopharmaceuticals Operations, Board of Radiation and Isotope Technology, New Bombay, India.
authors.forename: A
authors.initials: A
authors.lastname: Rathinaswamy
authors.forename: J
authors.initials: J
authors.lastname: Chatur
authors.forename: U H
authors.initials: UH
authors.lastname: Nagvekar
authors.forename: N
authors.initials: N
authors.lastname: Ramamoorthy
authors.forename: C N
authors.initials: CN
authors.lastname: Desai
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex; D011232:Chemical Precipitation; D007118:Immunoassay / Q000379:methods; D007457:Iodine Radioisotopes; D011092:Polyethylene Glycols
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007457:Iodine Radioisotopes; D011092:Polyethylene Glycols