Complex interaction of yeast nuclear proteins with the enhancer/promoter region of SV40.
Though highly complex enhancers found in animal cells have not been reported to occur in yeasts they are able to activate the transcription of adjacent genes in yeast cells. Saccharomyces cerevisiae expresses a large number of nuclear proteins that are able to recognize, and specifically bind to, the enhancer sequences of the SV40 animal tumor virus. The complexity of proteins that interact with different elements of the animal enhancers is similar in yeast and animal cell nuclear extracts. Most enhancer motifs, recognized by known trans-acting factors, are protected in footprinting experiments by yeast nuclear proteins.
Gyuris
J
J
Institute of Biochemistry, MTA Biological Research Center, Szeged, Hungary.
Dencsö
L
L
Polyák
K
K
Duda
E
E
D001483:Base Sequence; D004279:DNA, Viral / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004742:Enhancer Elements, Genetic; D005656:Fungal Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic
D004279:DNA, Viral; D005656:Fungal Proteins; D009687:Nuclear Proteins