title: New oligopeptide immunoglobulin G test for human parvovirus B19 antibodies.
abstract: A new, highly sensitive and specific enzyme immunoassay using oligopeptides as antigen (enzyme-linked immunosorbent assay [ELISA] B19-OP) for detecting parvovirus B19-specific immunoglobulin G (IgG) was established. As antigens, B19-specific oligopeptides of 24 and 30 kDa derived from a 196-kDa fusion protein of beta-galactosidase and viral capsid protein (VPI) of B19 after CNBr cleavage and separation by high-pressure liquid chromatography were used. Of 139 serum specimens tested in parallel for anti-B19 IgG by standard ELISA using B19 particles as antigen and by ELISA B19-OP, 73 (52.5%) were positive and 63 (45.3%) were negative in both tests, and 3 (2.2%) were negative by standard ELISA but positive by ELISA B19-OP and by immunoblot. By using ELISA B19-OP, it was possible to detect anti-B19 IgG in an asymptomatic blood donor 4 weeks after acute infection, and anti-B19 IgG titers of 10(-5) could be measured in convalescent-phase sera.
authors.affiliation: Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig-Maximilians-University of Munich, Federal Republic of Germany.
authors.forename: T F
authors.initials: TF
authors.lastname: Schwarz
authors.forename: S
authors.initials: S
authors.lastname: Modrow
authors.forename: B
authors.initials: B
authors.lastname: Hottenträger
authors.forename: B
authors.initials: B
authors.lastname: Höflacher
authors.forename: G
authors.initials: G
authors.lastname: Jäger
authors.forename: W
authors.initials: W
authors.lastname: Scharti
authors.forename: R
authors.initials: R
authors.lastname: Sumazakl
authors.forename: H
authors.initials: H
authors.lastname: Wolf
authors.forename: J
authors.initials: J
authors.lastname: Middeldorp
authors.forename: M
authors.initials: M
authors.lastname: Roggendorf
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D009842:Oligopeptides / Q000276:immunology; D010321:Parvoviridae / Q000276:immunology*; D010322:Parvoviridae Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D009842:Oligopeptides