Comparison of flow cytometry and virus isolation in cell culture for identification of cattle persistently infected with bovine viral diarrhea virus.
Detection of bovine viral diarrhea virus in 143 blood samples by virus isolation in cell culture and flow cytometry was performed. The material included 37 samples later shown to originate from persistently infected cattle. Thirty-three samples were positive by virus isolation, and all 37 samples were positive by the flow cytometric assay.
Qvist
P
P
Animal Biotechnology Research Center, National Veterinary Laboratory, Copenhagen, Denmark.
Houe
H
H
Aasted
B
B
Meyling
A
A
D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005434:Flow Cytometry; D012680:Sensitivity and Specificity; D014776:Virus Cultivation