title: Cloning and analysis of delta-endotoxin genes from Bacillus thuringiensis subsp. alesti.
abstract: Bacillus thuringiensis subsp. alesti produced only CryIA(b)-type protoxins, and three cryIA(b) genes were cloned. One was cryptic because of an alteration near the 5' end, and the other two were very similar to each other. The protoxin encoded by one of the latter genes differed from other CryIA(b) protoxins in its greater stability and relative toxicity for two members of the order Lepidoptera.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
authors.forename: C S
authors.initials: CS
authors.lastname: Lee
authors.forename: A I
authors.initials: AI
authors.lastname: Aronson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000472:pathogenicity; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004731:Endotoxins / Q000235:genetics* / Q000302:isolation & purification / Q000633:toxicity; D004926:Escherichia coli / Q000235:genetics; D006460:Hemolysin Proteins; D007915:Lepidoptera / Q000382:microbiology*; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011498:Protein Precursors; C030956:insecticidal crystal protein, Bacillus Thuringiensis