Each of the two productive T cell receptor alpha-gene rearrangements found in both the A10 and BM 3.3 T cell clones give rise to an alpha chain which can contribute to the constitution of a surface-expressed alpha beta dimer.
The H-2 Kb specific cytotoxic T cell clone BM3.3 carries two productive TCR alpha gene rearrangements but expresses a single detectable TCR alpha beta chain combination on its surface. However, when separately analyzed by gene transfer, both productive alpha gene rearrangements are translated into alpha chains capable of pairing with the BM3.3 beta chain and of cell surface expression. Similar observations have been made with the products of the two productive alpha gene rearrangements previously identified in the A10 T cell clone. These observations contrast with those made for the two TCR alpha chains expressed in the MS202 T cell clone. In the latter instance, when analyzed by gene transfer, one of the alpha chains was unable to make a pair with the beta chain. Consequently, when considered with respect to the mechanisms of TCR allelic exclusion, our data suggest that the mere expression of a TCR alpha beta dimer on the surface of immature CD4+ CD8+ thymocytes may be necessary but not sufficient to shutdown further alpha gene rearrangements. Rather, the ability of a TCR alpha beta dimer to be positively selected may be needed to trigger the maturation of thymocytes to a stage devoid of recombinase activity. Alternatively, if non-dividing CD4+ CD8+ CD3+ small thymocytes do not experience secondary alpha-gene rearrangement, our data suggest that TCR alpha gene rearrangements are attempted quasi-simultaneously on both alpha alleles.
Couez
D
D
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Marseille, France.
Malissen
M
M
Buferne
M
M
Schmitt-Verhulst
A M
AM
Malissen
B
B
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002999:Clone Cells / Q000276:immunology; D004247:DNA / Q000235:genetics; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection
D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA