title: Binding of a histidine-rich peptide to Porphyromonas gingivalis.
abstract: Porphyromonas gingivalis 381 cells were incubated with 125I-histidine-rich polypeptide (histatin) 5 in the presence or absence of unlabeled histatin 5, to evaluate the histatin-binding capacity of the cells. The binding of histatin 5 was rapid, reversible, saturable and specific. The number of histatin 5-binding sites per cell was 3,600, and the dissociation constant (Kd) was in the order of 10(-6) M. These findings suggest that histatin interacts with certain bacterial cells through specific binding sites on their surface, and will allow the development of a histatin radioreceptor assay.
authors.affiliation: Department of Preventive Dentistry, Faculty of Dentistry, Osaka University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: S
authors.initials: S
authors.lastname: Shizukuishi
authors.forename: A
authors.initials: A
authors.lastname: Tsunemitsu
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: S
authors.initials: S
authors.lastname: Aimoto
mesh_terms: D001667:Binding, Competitive; D054808:Histatins; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000378:metabolism*
chemical_list: C068075:HTN3 protein, human; D054808:Histatins; D008565:Membrane Proteins; D012471:Salivary Proteins and Peptides