title: Purification and properties of DNA gyrase from Vibrio cholerae.
abstract: The DNA gyrase was purified from Vibrio cholerae strain 569B. It appeared to be composed of two subunits of Mr 120,000 and 97,000 and had a great deal of similarity to the Escherichia coli gyrase. Unlike the E. coli enzyme, however, it could neither relax supercoiled DNA nor induce a cleavage of double-stranded DNA, under experimental conditions in which E. coli gyrase could do so.
authors.affiliation: Indian Institute of Chemical Biology, Calcutta.
authors.forename: G
authors.initials: G
authors.lastname: Mukhopadhyay
authors.forename: A
authors.initials: A
authors.lastname: Ghosh
mesh_terms: D004247:DNA / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism; D010093:Oxolinic Acid / Q000494:pharmacology; D010957:Plasmids; D013379:Substrate Specificity; D014734:Vibrio cholerae / Q000201:enzymology*
chemical_list: D004278:DNA, Superhelical; D004247:DNA; D004250:DNA Topoisomerases, Type II; D010093:Oxolinic Acid