[Changes in the membrane potential, the cytoplasmic Ca2+ concentration and in the transport of fluorescein anions across the macrophage plasma membrane under the influence of O2(-) and H2O2 in small concentrations].
It has been shown that under the influence of superoxide anion (300 nM) and hydrogen peroxide (100 nM) on murine peritoneal macrophages, the depolarization of membrane takes place, and a change in the rate of fluorescein anion efflux from the cells occurs. Hydrogen peroxide (but not superoxide anions) causes a transient increase in the cytoplasmic Ca2+ concentration. These changes are regarded as early signs of macrophage activation. It is assumed that macrophage activation with reactive oxygen intermediates at such a low concentration might be interpreted as follows: a small portion of stimulated phagocytes activates the whole population with the aid of the products of their own oxidative burst.
Gamaleĭ
I A
IA
Kaulin
A B
AB
Kirpichnikova
K M
KM
D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D003593:Cytoplasm / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005452:Fluoresceins / Q000493:pharmacokinetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D016897:Respiratory Burst / Q000187:drug effects / Q000502:physiology; D013481:Superoxides / Q000494:pharmacology*
D005452:Fluoresceins; D013481:Superoxides; D006861:Hydrogen Peroxide; D002118:Calcium
Izmenenie membrannogo potentsiala, kontsentratsii tsitoplazmaticheskogo Ca2+ i transporta anionov fluorestseina cherez plazmaticheskuiu membranu makrofagov pod vliianiem O2(-) i H2O2 v malykh kontsentratsiiakh.