title: Separation of human serum lipoproteins into three major classes by hydroxyapatite chromatography.
abstract: The separation of normolipidemic male serum lipoprotein fraction, prepared by ultracentrifugal flotation, was studied on hydroxyapatite columns. Potassium phosphate buffers in the pH range 5.6-7.4 were evaluated as eluents. The three main classes of the lipoproteins (high density, low density and very low density) can be separated on the Tiselius-type hydroxyapatite (Bio-Gel HTP DNA grade) column by elution with 75, 250 and 300 mM potassium phosphate buffer (pH 7.4), respectively.
authors.affiliation: Division of Analytical Chemistry, Tokyo College of Pharmacy, Japan.
authors.forename: U
authors.initials: U
authors.lastname: Matsumoto
authors.forename: H
authors.initials: H
authors.lastname: Nakayama
authors.forename: Y
authors.initials: Y
authors.lastname: Shibusawa
authors.forename: T
authors.initials: T
authors.lastname: Niimura
mesh_terms: D002021:Buffers; D002853:Chromatography, Liquid / Q000379:methods*; D017886:Durapatite; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D010710:Phosphates; D011188:Potassium; D017680:Potassium Compounds; D014461:Ultracentrifugation
chemical_list: D002021:Buffers; D006882:Hydroxyapatites; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D010710:Phosphates; D017680:Potassium Compounds; D017886:Durapatite; C013216:potassium phosphate; D011188:Potassium