The effect of glycosyl inositol-phosphate on cAMP production in isolated rat fat-cells is transduced by a pertussis toxin sensitive G-protein.
In fat-cells, insulin increases synergistically the inhibitory effects of adenosine and prostaglandin E2 on adenylyl cyclase activity. When the endogenous production of these feedback inhibitors is suppressed, insulin is no more active in conditions where glycosyl inositol-phosphate which is a putative mediator of its action, is always efficient. Moreover, glycosyl inositol-phosphate signal is transduced by a G protein sensitive to IAP intoxication.
Martiny
L
L
Laboratoire de Biochimie, UFR de Sciences Exactes et Naturelles, Reims, France.
Dib
K
K
Haye
B
B
Corrèze
C
C
Jacquemin
C
C
Lambert
B
B
D000241:Adenosine / Q000502:physiology; D037361:Adenylate Cyclase Toxin; D000273:Adipose Tissue / Q000166:cytology; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis*; D015232:Dinoprostone / Q000502:physiology; D019204:GTP-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D006017:Glycolipids / Q000502:physiology*; D017261:Glycosylphosphatidylinositols; D007328:Insulin / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D037342:Pertussis Toxin; D010716:Phosphatidylinositols / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D015398:Signal Transduction; D013552:Swine; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D037361:Adenylate Cyclase Toxin; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D007328:Insulin; D010716:Phosphatidylinositols; D010566:Virulence Factors, Bordetella; D000242:Cyclic AMP; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D000241:Adenosine; D015232:Dinoprostone