Phenotypic and genotypic analysis of Borrelia burgdorferi isolates from various sources.
A total of 17 B. burgdorferi isolates from various sources were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins, restriction enzyme analysis, Southern hybridization with probes complementary to unique regions of evolutionarily conserved genes (16S rRNA and fla), and direct sequencing of in vitro polymerase chain reaction-amplified fragments of the 16S rRNA gene. Three groups were distinguished on the basis of phenotypic and genotypic traits, the latter traced to the nucleotide sequence level.
Adam
T
T
Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, Federal Republic of Germany.
Gassmann
G S
GS
Rasiah
C
C
Göbel
U B
UB
D001426:Bacterial Proteins / Q000032:analysis; D001483:Base Sequence; D015139:Blotting, Southern; D015748:Borrelia burgdorferi Group / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004262:DNA Restriction Enzymes