title: Epstein-Barr virus and Hodgkin's disease. A correlative in situ hybridization and polymerase chain reaction study.
abstract: The authors studied typical Hodgkin's disease along with the nodular, lymphocyte predominance subtype by both the polymerase chain reaction (PCR) and in situ hybridization for evidence of the Epstein-Barr virus (EBV). By PCR, EBV DNA was detected in 12/23 cases of typical Hodgkin's disease and 2/13 cases of the nodular, lymphocyte predominance subtype. EBV RNA was detected by in situ hybridization studies within Reed-Sternberg cells and variants in 11/23 cases of typical Hodgkin's disease and 0/13 cases of nodular, lymphocyte predominance Hodgkin's disease. Other cells positive for EBV, identified as both B and T cells in double-labeling immunohistochemical/in situ hybridization studies, were found in 20/23 cases of typical Hodgkin's disease, 9/13 cases of nodular, lymphocyte predominance Hodgkin's disease, 4/6 cases of progressive transformation of germinal centers, and 7/10 normal lymphoid tissues. It is concluded that EBV is significantly associated with Reed-Sternberg cells in approximately one-half cases of typical Hodgkin's disease but not in the nodular, lymphocyte predominance subtype. EBV-infected B and T cells are also present in a majority of cases of Hodgkin's disease as well as in reactive conditions.
authors.affiliation: Department of Pathology, City of Hope National Medical Center, Duarte, CA 91010.
authors.forename: L M
authors.initials: LM
authors.lastname: Weiss
authors.forename: Y Y
authors.initials: YY
authors.lastname: Chen
authors.forename: X F
authors.initials: XF
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Shibata
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000382:microbiology* / Q000473:pathology; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D016539:Reed-Sternberg Cells / Q000737:chemistry
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral