Ca2+ or Mg2+ nucleotide phosphohydrolases in myometrium: two ecto-enzymes.
A high level of Ca2+ or Mg2+ nucleotide phosphohydrolase activity is present on the outside surface of intact myometrial cells and is also observed in the isolated plasma membranes. About half of this activity is labile while the remainder is stable. The characteristics of the activities suggest the presence of at least two different ecto-enzymes. The stable component (Km for Ca2+ about 0.1 mM) accepts XTP or XDP as substrate, is not inhibited by p-chloromercuriphenylsulfonate or inorganic phosphate, but is inhibited by 20 mM NaN3. The labile component (Km for Ca2+ nearly 1 mM) cleaves XTP but not XDP, and is inhibited by p-chloromercuriphenyl-sulfonate and inorganic phosphate, but not by NaN3. The activity of the labile component can be restored by removing the cells from the incubation medium and resuspending them in fresh medium. This suggests that the 'lability' is due to product inhibition, probably by inorganic orthophosphate. While the Ca2+ pump of myometrial plasma membranes was inhibited by 0.1 microM oxytocin, these ecto-enzymes were unaffected by oxytocin concentrations up to 10 microM. Because of its high activity and rapid inactivation by product inhibition, the labile enzyme may be involved in the regulation of purinergic receptors.
Magocsi
M
M
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905.
Penniston
J T
JT
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D003124:Colorimetry; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D009215:Myometrium / Q000166:cytology / Q000201:enzymology*; D010710:Phosphates / Q000494:pharmacology; D011247:Pregnancy; D011874:Radiometry; D051381:Rats; D013379:Substrate Specificity
D010710:Phosphates; D003208:Concanavalin A; D002731:4-Chloromercuribenzenesulfonate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases