Reactivity of monoclonal antibodies to the 41-kilodalton protein of porcine group C rotavirus with homologous and heterologous rotavirus serogroups in immunofluorescence tests.
Three monoclonal antibodies (MAbs) to porcine group C rotavirus immunoprecipitated the major inner capsid protein (41 kDa) but failed to precipitate group A rotavirus proteins. In immunofluorescence tests of rotavirus-infected cell cultures or pig intestines, the MAbs recognized porcine and bovine group C rotaviruses but not group A or B rotaviruses. These MAbs may recognize the group C rotavirus counterpart to VP6 of group A rotaviruses and may be useful as diagnostic reagents.
Ojeh
C K
CK
Department of Veterinary Microbiology and Parasitology, University of Ibadan, Nigeria.
Jiang
B M
BM
Tsunemitsu
H
H
Kang
S Y
SY
Weilnau
P A
PA
Saif
L J
LJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000918:Antibody Specificity; D002213:Capsid / Q000737:chemistry / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D051379:Mice; D008970:Molecular Weight; D012401:Rotavirus / Q000145:classification / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology; D012703:Serotyping; D013552:Swine
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral