Production of transferable neuronotrophic factor(s) by human midgut carcinoid tumour cells; studies using cultures of rat fetal cholinergic neurons.
A co-culture system was established between human midgut carcinoid tumour cells and rat fetal cholinergic neurons. In monocultures in serum-free media, only tumour cells survived, while neurons deteriorated. In serum-free co-cultures, neurons displayed outgrowth of neuritic processes. Neurons of neuronal serum-free monocultures thrived if supplemented with conditioned media from tumour cell cultures grown serum-free. This indicates that tumour cells produce transferable growth factor(s) with potent neuronotrophic actions. Immunocytochemical studies indicate that this growth factor resembles nerve growth factor immunologically, since tumour cells were strongly immunoreactive after incubation with a rabbit anti-nerve growth factor antiserum, and furthermore expressed immunoreactive nerve growth factor receptors.
Wigander
A
A
Institute of Neurobiology, NRCG, University of Gothenburg, Sweden.
Lundmark
K
K
McRae
A
A
Mölne
J
J
Nilsson
O
O
Haglid
K
K
Dahlström
A
A
Ahlman
H
H
D000818:Animals; D002276:Carcinoid Tumor / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D002799:Cholinergic Fibers / Q000187:drug effects / Q000648:ultrastructure*; D003470:Culture Media / Q000032:analysis / Q000494:pharmacology; D005260:Female; D006801:Humans; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D003470:Culture Media; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor