title: Nerve stimulation augments angiotensin II overflow from canine gracilis muscle in vivo.
abstract: The overflow of endogenous angiotensin-(1-8)octapeptide (angiotensin II) from blood-perfused canine gracilis muscle vasculature in situ was studied. A positive veno-arterial concentration difference for angiotensin II over the gracilis muscle was found, indicating a net generation of angiotensin II under basal conditions. Angiotensin II levels in the venous effluent were elevated during 2 Hz (4-min) nerve stimulation, suggesting enhanced local angiotensin II generation both in the presence and absence of alpha-adrenoceptor blockade. Thus, our results in this in vivo model demonstrate a local overflow of angiotensin II from the skeletal muscle vasculature which can be enhanced by nerve stimulation. Whether this overflow of angiotensin II is due to conversion of circulating angiotensin I to angiotensin II or local de novo synthesis of angiotensin II remains to be established.
authors.affiliation: Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: J H
authors.initials: JH
authors.lastname: Schwieler
authors.forename: J
authors.initials: J
authors.lastname: Nussberger
authors.forename: T
authors.initials: T
authors.lastname: Kahan
authors.forename: P
authors.initials: P
authors.lastname: Hjemdahl
mesh_terms: D000804:Angiotensin II / Q000378:metabolism*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D004285:Dogs; D004558:Electric Stimulation; D005260:Female; D009132:Muscles / Q000378:metabolism*; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects; D012084:Renin-Angiotensin System / Q000502:physiology; D013564:Sympathetic Nervous System / Q000502:physiology*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D001552:Benzazepines; D011942:Receptors, Adrenergic, alpha; D000804:Angiotensin II; C052133:benazeprilat