title: o-Succinylbenzoate: coenzyme A ligase, an enzyme involved in menaquinone (vitamin K2) biosynthesis, displays broad specificity.
abstract: o-Succinylbenzoate: coenzyme A ligase, an enzyme involved in menaquinone biosynthesis, was purified from Mycobacterium phlei and characterized with respect to isoelectric point, molecular weight, pH optimum, temperature optimum and kinetic data. The enzyme hydrolyses ATP to AMP. The substrate and cofactor specificity of the enzyme was tested with analogues of o-succinylbenzoic acid, different nucleotides, thiols and divalent cations. The enzyme appears to possess broad specificity for substrates and cofactors.
authors.affiliation: Institut für Pharmazeutische Biologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Bundesrepublik Deutschland.
authors.forename: H J
authors.initials: HJ
authors.lastname: Sieweke
authors.forename: E
authors.initials: E
authors.lastname: Leistner
mesh_terms: D002845:Chromatography; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D017886:Durapatite; D004789:Enzyme Activation; D006882:Hydroxyapatites; D007700:Kinetics; D009168:Mycobacterium phlei / Q000201:enzymology*; D013379:Substrate Specificity; D013389:Succinate-CoA Ligases / Q000302:isolation & purification / Q000378:metabolism*; D014812:Vitamin K / Q000096:biosynthesis*
chemical_list: D006882:Hydroxyapatites; D014812:Vitamin K; D017886:Durapatite; D013389:Succinate-CoA Ligases; C022693:O-succinylbenzoate - CoA ligase