title: Role of catecholamines in mediating messenger RNA and hormonal responses to stress.
abstract: The role of catecholamines in regulating the neuroendocrine stress response is controversial. We have investigated the effects of unilateral ventral noradrenergic bundle (VNAB) lesions on corticotrophin-releasing factor (CRF) and proenkephalin A mRNA responses in the parvocellular paraventricular nucleus (pPVN) to both physical and psychological stresses. We have also determined the effects of direct bilateral PVN lesions on CRF mRNA, plasma ACTH and corticosterone responses to psychological stress. 6-OHDA lesions whether to the VNAB or direct to the PVN did not result in any change in basal levels of CRF mRNA. Depletion of endogenous noradrenaline following unilateral lesions of the VNAB did not affect the CRF mRNA or the proenkephalin A mRNA response to stress. These data suggest that noradrenergic pathways are not involved in maintaining basal levels of CRF mRNA and that the noradrenergic input through the VNAB does not mediate the accumulation of CRF and proenkephalin A mRNAs in response to these stressors. Direct bilateral lesions to the PVN prevented the accumulation of CRF mRNA but not the ACTH and corticosterone responses to restraint stress. This suggests that monoamines are involved in the regulation of CRF mRNA through a mechanism independent of CRF-41 secretion.
authors.affiliation: Neuroendocrinology Unit, Charing Cross Hospital, London, U.K.
authors.forename: M S
authors.initials: MS
authors.lastname: Harbuz
authors.forename: H S
authors.initials: HS
authors.lastname: Chowdrey
authors.forename: D S
authors.initials: DS
authors.lastname: Jessop
authors.forename: S
authors.initials: S
authors.lastname: Biswas
authors.forename: S L
authors.initials: SL
authors.lastname: Lightman
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D002395:Catecholamines / Q000502:physiology*; D003345:Corticosterone / Q000097:blood; D003346:Corticotropin-Releasing Hormone / Q000235:genetics; D004745:Enkephalins / Q000097:blood; D006728:Hormones / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D016627:Oxidopamine / Q000494:pharmacology; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D011498:Protein Precursors / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013315:Stress, Psychological / Q000378:metabolism*
chemical_list: D002395:Catecholamines; D004745:Enkephalins; D006728:Hormones; D011498:Protein Precursors; D012333:RNA, Messenger; C029992:proenkephalin; D016627:Oxidopamine; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone; D009638:Norepinephrine