Antiviral activity of the prostanoid clavulone II against vesicular stomatitis virus.
Prostaglandins of the A series exhibit the most pronounced antiviral activity in cells infected with RNA or DNA viruses as compared to other prostaglandins. Clavulone is a prostaglandin A analog found in the soft coral Clavularia viridis. Using vesicular stomatitis virus in mouse L929 fibroblasts as a model system, 50% inhibition of viral yield was seen at a concentration of 1-1.5 microM, whereas 50% cytotoxicity required 50-70 times higher inhibitor concentrations. For a further elucidation of the antiviral mechanism a temperature-sensitive mutant, tsG 41, was used, which is replication-negative at the restrictive temperature. Results obtained with this mutant suggest that inhibition of VSV replication occurs at the level of transcription.
Bader
T
T
Medical College of Wisconsin, Department of Biochemistry, Milwaukee, Wisconsin 53226.
Yamada
Y
Y
Ankel
H
H
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D015152:Blotting, Northern; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects; D066298:In Vitro Techniques; D007739:L Cells / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D011454:Prostaglandins A / Q000737:chemistry / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects*; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D011454:Prostaglandins A; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C057741:clavulone II; C100573:prostaglandin A1