title: Interleukin-1 alpha-induced changes in androgen and cyclic adenosine 3',5'-monophosphate release in adult rat Leydig cells in culture.
abstract: Interleukin-1 (IL-1) has been proposed as a paracrine regulator of testicular function. The effect of this cytokine on adult rat Leydig cells in primary culture was investigated. Interstitial cells were purified on a two-step Percoll gradient and cultured in the presence or absence of recombinant human IL-1 alpha (IL-1 alpha). The presence of IL-1 alpha in the culture media resulted in a dose-dependent increase in 24-h basal androgen release (half-maximal effective concentration = 40-50 U/ml). The stimulatory effect of IL-1 alpha peaked on days 3 and 4, and was often still significant after 6 days of culture. Removal of IL-1 alpha was followed by a return of basal androgen release to control levels within 3 days. In contrast, cells treated with IL-1 alpha (100 U/ml) for 3 days released significantly less androgen (per 4 h) in response to 1-100 ng LH/ml than control cells. A similar inhibitory effect on the response to dibutyryl cAMP and pregnenolone was observed. Under basal conditions, IL-1 alpha-treated cells released significantly more cAMP than did control cells. In contrast, the increase in cAMP release seen with LH-stimulated cells was significantly inhibited by treatment with IL-1 alpha. These results suggest that IL-1 alpha has a dual effect on adult rat Leydig cells in culture. It stimulates basal but inhibits LH-induced androgen release with parallel changes in cAMP levels. An additional inhibitory effect appears to lie at the level of the 17 alpha-hydroxylase/C17-20 lyase enzyme.
authors.affiliation: Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Moore
authors.forename: W H
authors.initials: WH
authors.lastname: Moger
mesh_terms: D000728:Androgens / Q000096:biosynthesis*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000494:pharmacology*; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D011284:Pregnenolone / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D000728:Androgens; D007375:Interleukin-1; D003994:Bucladesine; D011284:Pregnenolone; D007986:Luteinizing Hormone; D000242:Cyclic AMP