title: Cortisol-induced changes in some aspects of the intermediary metabolism of Salvelinus fontinalis.
abstract: Cortisol was administered to brook charr (Salvelinus fontinalis) in the form of slow-release intraperitoneal implants (a) to investigate the effect of chronic cortisol stimulation (up to 60 days) on various aspects of intermediary metabolism, and (b) to determine whether such cortisol-induced changes were comparable to those seen in chronically fasted charr. Except for fish sampled at 1 and 6 h after implantation, there was no consistent increase in the plasma cortisol levels of the cortisol-implanted animals. Nevertheless, there were significant treatment effects (particularly after 60 days) on certain metabolite levels, and key hepatic enzymes, including a lowering of plasma glucose and hepatic glycogen concentrations, increased activities of FBPase, G6PDH, GK, and G3PDH, and reduced activities of PFK. When taken together these changes are indicative of a direct or indirect gluconeogenic action of cortisol, in which metabolites other than amino acids (possibly glycerol) are utilized as substrates. These metabolic changes differed from those found in food-deprived brook charr which appeared to use proteins, as well as lipids, as energy sources.
authors.affiliation: Department of Zoology, University of Guelph, Ontario, Canada.
authors.forename: M M
authors.initials: MM
authors.lastname: Vijayan
authors.forename: J S
authors.initials: JS
authors.lastname: Ballantyne
authors.forename: J F
authors.initials: JF
authors.lastname: Leatherland
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004343:Drug Implants; D005508:Food Deprivation; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D005991:Glycerol Kinase / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006854:Hydrocortisone / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000378:metabolism; D014337:Trout / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D004343:Drug Implants; D006003:Glycogen; D005954:Glucosephosphate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010732:Phosphofructokinase-1; D005991:Glycerol Kinase; D006597:Fructose-Bisphosphatase; D006854:Hydrocortisone