[Inhibitory effects of enzymolytic product from normal human plasma on hematogenous metastasis of murine gastric carcinoma cells].
Enzymolytic product (EP) of normal human plasma (NHP), was prepared through combined digestion of alpha-chymotrypsin and pepsin. This product was studied with MGc 80-3 cells (human gastric carcinoma cell line) and FC cells (615-murine gastric carcinoma cell line). The adhesive rate of NHP to MGc 80-3 cells may reach 90%, while the capacity of NHP-EP to inhibit adhesion reached over 90%, being specific and non-cytotoxic. Intravenous coinjection of NHP-EP with FC murine gastric carcinoma cells remarkably inhibited the formation of lung tubercles in 615-mice, with an inhibitory rate of 83.3% and a median survival time prolonged 35 days, which was only 19 days in the controls. The results showed that NHP-EP can inhibit experimental hematogenous metastasis through interfering the adhesion of tumor cells to extracellular stroma. We believe that NHP-EP may have practical value in preventing, seeding of metastatic cells after surgical removal of a primary tumor.
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D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D002448:Cell Adhesion; D005260:Female; D005353:Fibronectins / Q000502:physiology; D006801:Humans; D007797:Laminin / Q000502:physiology; D008297:Male; D051379:Mice; D009088:Mucoproteins / Q000502:physiology; D009368:Neoplasm Transplantation; D009360:Neoplastic Cells, Circulating; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D001798:Blood Proteins; D005353:Fibronectins; D007797:Laminin; D009088:Mucoproteins