[Phosphorylation of myocardial sarcolemma proteins during induction of the phosphatidylinositol cycle in isolated perfused rabbit heart].
Phosphorylation of cardiac sarcolemma proteins under stimulation of M-receptors by agonist carbacholine used to stimulate phosphatidylinositide cycle, was investigated in the isolated, rabbit heart perfused with 32Pi. Carbacholine (10(-7) stimulates the polyphosphoinositide metabolism which is expressed in the activated incorporation of 32P from [gamma-32P]ATP in polyphosphoinositide as well as in the increased content of the labelled inositol trisphosphate released through phosphatidylinositol-4,5-bisphosphate break-down by phospholipase C. The diacylglycerol produced simultaneously with inositol triphosphate as a second messenger activates the protein kinase C. This was confirmed by considerable activation of phosphorylation sarcolemma proteins-substrates of protein kinase C, with Mr 94, 87, 78, 51 and 46 kDa.
Kurskiĭ
M D
MD
Kocherga
V I
VI
Puzyrev
S Iu
SIu
Nesterenko
N V
NV
Kurchenko
L K
LK
Afanas'ev
V A
VA
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004075:Diglycerides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D011817:Rabbits; D012508:Sarcolemma / Q000201:enzymology / Q000378:metabolism*
D004075:Diglycerides; D010716:Phosphatidylinositols; D000255:Adenosine Triphosphate; D002217:Carbachol; D011493:Protein Kinase C
Fosforilirovanie belkov sarkolemmy miokarda pri induktsii fosfatidilinozitidnogo tsikla v izolirovannom perfuziruemom serdtse krolika.