Evidence of absence of Trypanosoma cruzi kinetoplast DNA methylation by restriction endonuclease analysis.
Eight kinetoplast DNA samples from very different T. cruzi zymodemes were digested with the isoschizomer group of enzymes (MspI-HpaII) and (MboI-Sau 3AI), able to detect DNA methylation on cytidine and adenine for the CCGG and GATC sequences, respectively. Restriction digestion analysis of each kDNA with both isoschizomer groups of enzymes did not display a different profile suggesting that maxicircles and minicircles on this trypanosomatid are not methylated.
Muñoz
S
S
Departamento de Bioquimica, Facultad de Medicina, Universidad de Chile, Santiago.
Venegas
J
J
Solari
A
A
D000818:Animals; D004270:DNA, Circular / Q000378:metabolism*; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan / Q000378:metabolism*; D008745:Methylation; D015183:Restriction Mapping; D014349:Trypanosoma cruzi / Q000235:genetics*
D004270:DNA, Circular; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan