Characterization of encephalomyocarditis virus isolated from aborted swine fetuses.
Characteristics of 2 encephalomyocarditis virus (EMCV) isolates (MN-25 and MN-30) recovered from aborted swine fetuses were examined along with 2 other swine isolates (NVSL-MDV and NVSL-PR) and a reference ATCC strain (VR-129). All 5 EMCV isolates were found to be serologically related by cross testing, using serum neutralization and fluorescent antibody assays. Hemagglutination (HA) properties of the 5 isolates were compared, using 5 diluents. The MN-25 and NVSL-MDV strains had HA activity with guinea pig RBC in all 5 diluents, whereas MN-30, NVSL-PR, and VR-129 had HA activity only in KCl-borate buffer. The HA ability with RBC of various animal species was examined, using KCl-borate diluent. All virus isolates had high HA titer (1:512 to 1:2,048) with guinea pig, rat, and horse RBC and lower HA titer (1:16 to 1:64) with sheep RBC. The MN-25 and NVSL-MDV isolates agglutinated dogs RBC, whereas MN-30, NVSL-PR, and VR-129 strains did not. Viral replication was evident in 8 of 10 cell lines tested, although infectivity titers of each virus varied by cell line used. Plaque-forming ability was similar for all 5 isolates, but plaque size was different by virus and cell culture used. Virus isolates were found to be stable after being heated at 56 C and subjected to a wide range of pH. A viral polypeptide pattern difference for all 5 isolates was not found by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis.(ABSTRACT TRUNCATED AT 250 WORDS)
Kim
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HS
Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St Paul 55108.
Christianson
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WT
Joo
H S
HS
D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004686:Encephalomyocarditis virus / Q000737:chemistry / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D004769:Enterovirus Infections / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D005333:Fetus / Q000382:microbiology*; D006367:HeLa Cells; D006386:Hemagglutination Tests; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D009500:Neutralization Tests; D011247:Pregnancy; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis
D014764:Viral Proteins