pp54 microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity.
pp54 microtubule-associated protein-2 (MAP-2) kinase, a recently discovered protein serine/threonine kinase (Kyriakis, J., and Avruch, J. (1990) J. Biol. Chem. 265, 17355-17363), is shown to contain immunoreactive phosphotyrosine residues. Treatment with recombinant rat brain protein tyrosine phosphatase-1 deactivates pp54 MAP-2 kinase, concomitant with the removal of phosphotyrosine residues. Protein (serine/threonine) phosphatase-1 also deactivates pp54 MAP-2 kinase in a specific fashion. pp54 MAP-2 kinase joins pp42 MAP-2 kinase and cdc2/maturation-promoting factor as one of only three serine/threonine protein kinases known to be regulated by phosphorylation at both tyrosine and, independently, at serine/threonine residues. In view of these shared regulatory properties, a role for pp54 MAP-2 kinase in the control of cell division is likely.
Kyriakis
J M
JM
Diabetes Unit and Medical Services, Massachusetts General Hospital, Boston.
Brautigan
D L
DL
Ingebritsen
T S
TS
Avruch
J
J
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology; D016203:CDC2 Protein Kinase / Q000378:metabolism; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D010749:Phosphoprotein Phosphatases / Q000494:pharmacology; D010766:Phosphorylation; D011233:Precipitin Tests; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D054645:Protein Phosphatase 1; D011817:Rabbits; D051381:Rats; D012694:Serine / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
D047428:Protein Kinase Inhibitors; D013912:Threonine; D014443:Tyrosine; D012694:Serine; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D016203:CDC2 Protein Kinase; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1