Requirement of heparan sulfate for bFGF-mediated fibroblast growth and myoblast differentiation.
Basic fibroblast growth factor (bFGF) binds to heparan sulfate proteoglycans at the cell surface and to receptors with tyrosine kinase activity. Prevention of binding between cell surface heparan sulfate and bFGF (i) substantially reduces binding of fibroblast growth factor to its cell-surface receptors, (ii) blocks the ability of bFGF to support the growth of Swiss 3T3 fibroblasts, and (iii) induces terminal differentiation of MM14 skeletal muscle cells, which is normally repressed by fibroblast growth factor. These results indicate that cell surface heparan sulfate is directly involved in bFGF cell signaling.
Rapraeger
A C
AC
Department of Pathology, University of Wisconsin, Madison 53706.
Krufka
A
A
Olwin
B B
BB
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002704:Chlorates / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology*; D006497:Heparan Sulfate / Q000502:physiology*; D066298:In Vitro Techniques; D051379:Mice; D009132:Muscles / Q000166:cytology*; D011133:Polysaccharide-Lyases / Q000494:pharmacology; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism; D013329:Structure-Activity Relationship
D002704:Chlorates; D016222:Fibroblast Growth Factor 2; D006497:Heparan Sulfate; D011133:Polysaccharide-Lyases; D011956:Receptors, Cell Surface; C021349:heparitinsulfate lyase; C032706:sodium chlorate