Purification and properties of DNA gyrase from Vibrio cholerae.
The DNA gyrase was purified from Vibrio cholerae strain 569B. It appeared to be composed of two subunits of Mr 120,000 and 97,000 and had a great deal of similarity to the Escherichia coli gyrase. Unlike the E. coli enzyme, however, it could neither relax supercoiled DNA nor induce a cleavage of double-stranded DNA, under experimental conditions in which E. coli gyrase could do so.
Mukhopadhyay
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G
Indian Institute of Chemical Biology, Calcutta.
Ghosh
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A
D004247:DNA / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism; D010093:Oxolinic Acid / Q000494:pharmacology; D010957:Plasmids; D013379:Substrate Specificity; D014734:Vibrio cholerae / Q000201:enzymology*
D004278:DNA, Superhelical; D004247:DNA; D004250:DNA Topoisomerases, Type II; D010093:Oxolinic Acid