Quantification of concentration changes in neonatal human cerebral oxidized cytochrome oxidase.
The oxygenation of cerebral cytochrome oxidase in vivo was investigated in eight newborn preterm infants. Near-infrared spectroscopy was used to quantify changes in the concentration of oxidized cytochrome oxidase ([CytO2]) observed during alterations in arterial oxygen saturation (SaO2) in the range of 85-99% and of carbon dioxide tension (PaCO2) in the range of 4.3-9.6 kPa. No relation was found between changes in SaO2 and [CytO2]. Alterations in PaCO2 were positively related both to changes in [CytO2] and total cerebral hemoglobin concentration [( Hb]t). The changes in [CytO2] ranged from 0.09 to 0.33 (median 0.21) mumol.l-1.kPa-1. The ratio [CytO2]/[Hb]t ranged from 0.06 to 0.12 (median 0.08). The relation of delta [CytO2] to the change in cerebral blood volume (delta CBV) was calculated: delta [CytO2]/delta CBV ranged from 0.09 to 0.18 (median 0.11) mumol/ml. These results define a fraction of cerebral cytochrome oxidase in the newborn infant that is oxidized after an increase in PaCO2 but demonstrate that a change in SaO2 in the range studied was not sufficient by itself to change [CytO2]. They differ from results of studies in adults; this may reflect significant differences between adult and neonatal brain.
Edwards
A D
AD
Department of Paediatrics, University College and Middlesex School of Medicine, London, United Kingdom.
Brown
G C
GC
Cope
M
M
Wyatt
J S
JS
McCormick
D C
DC
Roth
S C
SC
Delpy
D T
DT
Reynolds
E O
EO
D001921:Brain / Q000201:enzymology*; D002245:Carbon Dioxide / Q000097:blood; D002560:Cerebrovascular Circulation; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D008297:Male; D010084:Oxidation-Reduction; D010100:Oxygen / Q000097:blood; D013055:Spectrophotometry, Infrared
D006454:Hemoglobins; D002245:Carbon Dioxide; D003576:Electron Transport Complex IV; D010100:Oxygen