title: Inhibition of adhesion-promoting activity of a human salivary protein which promotes adhesion of Streptococcus mutans JBP to hydroxyapatite.
abstract: The adhesion-promoting proteins (APP) (molecular mass approx. 300 kDa), which promote adhesion of Streptococcus mutans JBP (serotype c) to hydroxyapatite, were isolated from human submandibular-sublingual (SMSL) saliva by gel filtration on a Trisacryl GP2000 M column. The effects of hexoses, pentoses, methyl-pentoses, hexosamines, N-acetylhexosamines, a basic amino acid, polyamines and ammonium chloride on the bacterial adhesion-promoting activity of the APP were examined. Galactosamine, mannosamine, L-lysine, spermine, putrescine, and ammonium chloride inhibited the adhesion-promoting activities of the APP. The other sugars, including the N-acetylhexosamines, were without effect. Thus, compounds containing a primary amino-group appear to have a specific inhibitory effect on adhesion of S. mutans JBP to APP adsorbed onto hydroxyapatite, an activity which is lost if the amino-group is acetylated.
authors.affiliation: Department of Preventive Dentistry, Okayama University Dental School, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Kishimoto
authors.forename: D I
authors.initials: DI
authors.lastname: Hay
authors.forename: R J
authors.initials: RJ
authors.lastname: Gibbons
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D002241:Carbohydrates / Q000494:pharmacology; D017886:Durapatite; D006801:Humans; D006882:Hydroxyapatites; D008297:Male; D008970:Molecular Weight; D009077:Mucins / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000037:antagonists & inhibitors*; D013295:Streptococcus mutans / Q000502:physiology*
chemical_list: D002241:Carbohydrates; D006882:Hydroxyapatites; D009077:Mucins; D012471:Salivary Proteins and Peptides; D017886:Durapatite