Thomsen-Friedenreich (T)-active glycoproteins, blood group N antigen precursor glycoproteins and N antigen precursor glycoproteins with T activity from ascitic fluids of liver cancer patients.
1. Five perchloric acid-soluble fractions (PASFs) obtained from ascitic fluids of three patients with primary hepatocellular carcinoma (HCC), a patient with liver metastatic carcinoma (LUC) from ureteral carcinoma and human normal serum (NS) were subjected to DEAE-cellulose column chromatography to separate seven glycoprotein fractions, respectively. 2. In this chromatography, two HCC-PASFs gave a Thomsen-Friedenreich (T)-active glycoprotein, respectively. 3. Other HCC-PASF gave a T-active glycoprotein, two blood group N antigen precursor glycoproteins and an N antigen precursor glycoprotein with T activity. 4. LUC-PASF gave two T-active glycoproteins and an N antigen precursor glycoprotein with T activity. 5. NS-PASF did not give these serologically active glycoproteins.
Otsuka
K
K
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Nishida
S
S
Yuasa
H
H
Hara
A
A
Shirai
T
T
Ohkuma
S
S
D000596:Amino Acids / Q000032:analysis; D001202:Ascitic Fluid / Q000737:chemistry / Q000378:metabolism*; D001789:Blood Group Antigens; D006528:Carcinoma, Hepatocellular / Q000737:chemistry* / Q000556:secondary; D002848:Chromatography, DEAE-Cellulose; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007519:Isoantigens / Q000737:chemistry / Q000378:metabolism*; D008113:Liver Neoplasms / Q000737:chemistry* / Q000556:secondary; D010472:Perchlorates / Q000737:chemistry; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*; D014312:Trisaccharides / Q000737:chemistry / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000473:pathology
D000596:Amino Acids; D001789:Blood Group Antigens; D007519:Isoantigens; D010472:Perchlorates; D011498:Protein Precursors; C064068:Thomsen-Friedenreich antigen, cryptic; D014312:Trisaccharides