title: Purification and partial characterization of rat liver pyruvate dehydrogenase kinase activator protein (free pyruvate dehydrogenase kinase).
abstract: Rat liver pyruvate dehydrogenase (PDH) kinase activator protein (KAP), a free PDH kinase readily separable from PDH complex and its intrinsic kinase, has been purified to apparent homogeneity from liver mitochondria of fed and 48-h starved rats. On SDS-PAGE an apparently single band of M(r) 45 kDa was obtained. N-Terminal amino acid sequence analyses (8-10 cycles) confirmed the presence of a single peptide in each case. The specific activity of the purified KAP from 48-h starved rats (14,413 U/mg protein) was 4.5-fold greater than that from fed rats.
authors.affiliation: Department of Clinical Biochemistry, University of Oxford, John Radcliffe Hospital, UK.
authors.forename: D A
authors.initials: DA
authors.lastname: Priestman
authors.forename: S C
authors.initials: SC
authors.lastname: Mistry
authors.forename: A L
authors.initials: AL
authors.lastname: Kerbey
authors.forename: P J
authors.initials: PJ
authors.lastname: Randle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D051381:Rats; D013217:Starvation / Q000201:enzymology
chemical_list: D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases