title: Gene rearrangement in B- and T-lymphoproliferative disease detected by the polymerase chain reaction.
abstract: Gene rearrangement and monoclonality have been detected in normal cells and in lymphoproliferative disease by using the polymerase chain reaction and primers for the V and J regions of the Ig heavy chain gene or T-cell receptor gamma-chain gene. Using the Ig primers monoclonality was detected in 20 of 20 normal B-lymphocyte clones and in 39 of 52 cases of various types of B-lymphoproliferative disease, but not in 11 cases of T-lymphoproliferative disease. Using the T-cell receptor primers, monoclonality was detected in 186 of 192 normal T-lymphocyte clones, in 11 of 11 cases of T-lymphoproliferative disease, in 9 of 12 cases of B-acute lymphocytic leukemia, and in 1 of 21 cases of B-non-Hodgkin's lymphoma, but not in nine cases of B-chronic lymphocytic leukemia nor in 10 cases of myeloma. Monoclonality was detected in material obtained by lymph node aspiration in four of six additional cases of non-Hodgkin's lymphoma. It was not detected in 10 cases of acute myeloid leukemia nor in four cases of reactive lymphadenopathy. Detection of gene rearrangement by the polymerase chain reaction has a number of advantages over Southern blotting and is likely to become the initial diagnostic technique of choice to detect monoclonality.
authors.affiliation: Department of Haematology, Flinders Medical Centre, Bedford Park, South Australia.
authors.forename: K J
authors.initials: KJ
authors.lastname: Trainor
authors.forename: M J
authors.initials: MJ
authors.lastname: Brisco
authors.forename: J H
authors.initials: JH
authors.lastname: Wan
authors.forename: S
authors.initials: S
authors.lastname: Neoh
authors.forename: S
authors.initials: S
authors.lastname: Grist
authors.forename: A A
authors.initials: AA
authors.lastname: Morley
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000473:pathology; D001483:Base Sequence; D015139:Blotting, Southern; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000473:pathology / Q000648:ultrastructure; D008232:Lymphoproliferative Disorders / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell