Cloning of human heterohybridoma cell lines using chronic lymphocytic leukemia B cells as a feeder layer.
B cells from the peripheral blood of chronic lymphocytic leukemia patients were isolated by gradient density centrifugation and used without irradiation as a feeder layer in the cloning of human heterohybridoma cell lines by limiting dilution. Cloning efficiencies were high with all the cell lines tested. These feeder leukemia B cells could also be successfully used after having been stored in liquid nitrogen.
Sanchez
B
B
Servicio de Immunología, Hospital Universitario Virgen del Rocío, Sevilla, Spain.
Robledo
M M
MM
Yelamos
J
J
Gonzalez
M F
MF
Magariño
R
R
Bermudo
M P
MP
Nuñez-Roldan
A
A
D000818:Animals; D002472:Cell Transformation, Viral; D002999:Clone Cells; D004854:Herpesvirus 4, Human; D006801:Humans; D006822:Hybrid Cells; D006825:Hybridomas / Q000166:cytology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology; D051379:Mice; D014407:Tumor Cells, Cultured