Electrolyte transport across the pleura of rabbits.
The amounts of Na+ and Cl- in the right pleural space of anesthetized rabbits were determined 10 and 60 min after a 2 ml hydrothorax with the following solutions: Ringer, Ringer with an inhibitor of the Na(+)-Cl- coupled transport or of the Na+/K+ pump, Ringer with gluconate instead of Cl- or with methylglucamine instead of Na+. During the 10-60 min period: (a) with Ringer Na+ and Cl- decreased (P less than 0.01) along with an iso-osmotic liquid absorption, (b) with disulfonic-stilbene (0.1 mM), amiloride (0.7 mM), acetazolamide (0.1 mM), or ouabain (0.5 mM) Na+ did not change and Cl- decreased less (P less than 0.01) than with Ringer. With gluconate-Ringer or methylglucamine-Ringer the liquid flow reversed: in the former case Cl- and, to a smaller extent, Na+ increased (P less than 0.01); in the latter only Na+ increased (P less than 0.01). These findings suggest: (1) the occurrence of a Na+/H+ and Cl-/HCO3- double exchange on the serosal side and of a Na+/K+ pump on the interstitial side of the pleural mesothelium; (2) a slow efflux from the pleural space of gluconate or methylglucamine relative to the corresponding influx of Cl- or Na+, respectively; this drags liquid into the space by osmotic gradient.
Zocchi
L
L
Istituto di Fisiologia umana I, Università di Milano, Italy.
Agostoni
E
E
Cremaschi
D
D
D000086:Acetazolamide / Q000494:pharmacology; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001639:Bicarbonates / Q000493:pharmacokinetics; D001692:Biological Transport / Q000502:physiology; D002034:Bumetanide / Q000493:pharmacokinetics; D004573:Electrolytes / Q000493:pharmacokinetics*; D005942:Gluconates / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D008536:Meglumine / Q000493:pharmacokinetics; D010042:Ouabain / Q000494:pharmacology; D010994:Pleura / Q000502:physiology*; D011188:Potassium / Q000493:pharmacokinetics; D011817:Rabbits; D012964:Sodium / Q000493:pharmacokinetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000502:physiology; D014882:Water-Electrolyte Balance / Q000502:physiology*
D001639:Bicarbonates; D004573:Electrolytes; D005942:Gluconates; D002034:Bumetanide; D010042:Ouabain; D008536:Meglumine; D000584:Amiloride; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D000086:Acetazolamide; C030691:gluconic acid; D011188:Potassium