Comparison of staphylococcal co-agglutination with other assays for rapid diagnosis of rotavirus infection in humans, calves and piglets.
Co-agglutination was used for rapid diagnosis of rotavirus infection. Staphylococcus aureus Cowan I sensitized with rabbit antiserum to rotavirus demonstrated the presence of this virus in stools of children, calves and piglets with clinical signs of diarrhea. A total of 415 fecal samples were examined by the co-agglutination test and the results were compared with those of ELISA, electrophoresis in polyacrylamide gel and latex agglutination. The co-agglutination test, when compared to these three methods, presented a correlation which varied between 87.2 and 94.2%.
Durigon
E L
EL
Laboratories of Virology, University of São Paulo, Brazil.
Candeias
J A
JA
Jerez
J A
JA
Bittencourt
M J
MJ
Ortolani
E L
EL
D000256:Adenoviridae / Q000276:immunology; D000257:Adenoviridae Infections / Q000175:diagnosis; D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D002417:Cattle; D005243:Feces / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus; D013552:Swine