title: Thrombin-induced breakdown of phosphoinositides in platelets from patients with NIDDM.
abstract: We have examined thrombin-induced metabolism of phosphoinositides in the platelets from fifteen NIDDM (non-insulin-dependent diabetes mellitus) patients and fifteen healthy subjects (control). The diabetic patients were divided into two groups. One group (group I) had diabetic retinopathy (microangiopathy) and the other group (group II) had atherosclerosis of great vessels (macroangiopathy). In platelets incubated with [32P] orthophosphate for 80 min, the incorporation of 32P radioactivity into phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) was significantly lower in the group II than in the control. The addition of thrombin induced a marked decrease in PIP2 radioactivity at 10 sec in platelets from group I compared with that from the control. These results suggest that the breakdown of polyphosphoinositides is increased in platelets from diabetic subjects with retinopathy, and also that the formation of polyphosphoinositides is decreased in the platelets from diabetic subjects with macroangiopathy.
authors.affiliation: Third Department of Internal Medicine, Gifu University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishizuka
authors.forename: K
authors.initials: K
authors.lastname: Yasuda
authors.forename: N
authors.initials: N
authors.lastname: Takeda
authors.forename: K
authors.initials: K
authors.lastname: Kajita
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: S
authors.initials: S
authors.lastname: Nakashima
authors.forename: S
authors.initials: S
authors.lastname: Nagao
authors.forename: Y
authors.initials: Y
authors.lastname: Nozawa
mesh_terms: D000328:Adult; D000368:Aged; D001786:Blood Glucose; D001792:Blood Platelets / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D003324:Coronary Artery Disease / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000503:physiopathology*; D003930:Diabetic Retinopathy / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010712:Phosphatidic Acids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology*; D013997:Time Factors; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D014280:Triglycerides; D002784:Cholesterol; D013917:Thrombin