A dipstick immunobinding enzyme-linked immunosorbent assay for serodiagnosis of hepatitis B and delta virus infections.
A simple, specific and economical dipstick immunobinding enzyme-linked immunosorbent assay (DIA) for detecting hepatitis B surface antigen (HBsAg) and antibodies to hepatitis delta virus (anti-HDV), utilizing cellulose nitrate membrane is described. Screening of 815 serum specimens for HBsAg by DIA and micro ELISA revealed a positivity of 22.69% and 22.94% respectively. In the detection of antibodies to delta antigen, DIA was compared with an indirect immunofluorescence technique using A3 cell line as antigen substrate and a commercial macro ELISA. Of the 143 HBsAg positive sera tested for anti-HDV, 59 (41.25%) were positive by both immunofluorescence and macro ELISA and 61 (42.65%) by DIA. While the positive and negative predictive values of DIA for HBsAg were 100% and 99.6%, for anti-HDV by DIA these were 96.7% and 100% respectively. Based on the simplicity of performance and the economical nature of the test system, DIA is recommended as a diagnostic tool for field surveys and small laboratories in developing countries.
Sumathy
S
S
Department of Microbiology, Dr. A. L. M. Post Graduate Institute of Basic Medical Sciences, Taramani, Madras, India.
Thyagarajan
S P
SP
Latif
R
R
Madanagopalan
N
N
Raguram
K
K
Rajasambandam
P
P
Gowans
E
E
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006508:Hepatitis Antibodies / Q000097:blood*; D006509:Hepatitis B / Q000175:diagnosis*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D003699:Hepatitis D / Q000175:diagnosis*; D006801:Humans; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D006508:Hepatitis Antibodies; D006514:Hepatitis B Surface Antigens