Functional characterization of SV40-transformed adherent synovial cells from rheumatoid arthritis.
A total of 14 transformed cell clones were obtained by micro-injecting origin-defective SV40 DNA into three types of cloned adherent synovial cells (ASC) (dendritic cells (DCs), macrophage-like cells (MCs), and fibroblast-like cells (FCs)) from two rheumatoid arthritis patients (five DC clones (SV40-DCs), five MC clones (SV40-MCs) and four FC clones (SV40-FCs)). All the transformed cell nuclei expressed SV40-specific T antigen. The cells which formed a colony had a few times shorter doubling time than the original cells. IL-1 alpha, IL-1 beta and prostaglandin E2 were detected in the culture supernatant from the unstimulated transformed cells like untransformed cells. The SV40-DCs showed the most potent accessory cell function in oxidative mitogenesis assay among the three types of SV40-ASCs. Granulocyte macrophage colony stimulatory factor (GM-CSF) was detected only in the culture supernatant from the SV40-MCs without stimulation. Extensive phenotypic analysis revealed relatively cell-specific markers. SV40-DCs were HLA-DP+ and glial fibrillary acidic protein positive. SV40-MCs stained positive for 5'-nucleotidase and nonspecific esterase. These transformed ASCs retained much of the original cellular physiology of rheumatoid arthritis (RA) ASCs and may be a useful tool for characterizing the role of ASCs in the pathogenesis of RA.
Goto
M
M
Division of Rheumatic Diseases, Tokyo Metropolitan Otsuka Hospital, Japan.
Okamoto
M
M
Sasano
M
M
Nishizawa
K
K
Aotsuka
S
S
Yamaguchi
N
N
Obinata
M
M
Ikeda
K
K
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D015153:Blotting, Western; D002455:Cell Division / Q000235:genetics; D002472:Cell Transformation, Viral; D002999:Clone Cells; D003713:Dendritic Cells / Q000276:immunology*; D015232:Dinoprostone / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006801:Humans; D016130:Immunophenotyping; D007375:Interleukin-1 / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology; D008845:Microinjections; D008875:Middle Aged; D013539:Simian virus 40; D013583:Synovial Membrane / Q000166:cytology*
D007375:Interleukin-1; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015232:Dinoprostone