In vivo detection of metabolic changes in a mouse model of scrapie using nuclear magnetic resonance spectroscopy.
In vivo proton nuclear magnetic resonance (NMR) spectroscopy studies of scrapie in a mouse model have shown the appearance of an abnormal peak in the brain early in the incubation period. This abnormal peak was detected weeks before the detection of a protease-resistant form of a membrane protein and vacuolar histopathology in vitro, and several months before clinical signs, and the signal increased in intensity as the disease progressed. In the chronic stage of the disease, a reduction in N-acetyl aspartate levels was observed using in vivo and in vitro proton NMR spectroscopy.
Bell
J D
JD
NMR Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
Cox
I J
IJ
Williams
S C
SC
Belton
P S
PS
McConnell
I
I
Hope
J
J
D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D004195:Disease Models, Animal; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D010472:Perchlorates / Q000737:chemistry / Q000378:metabolism; D012608:Scrapie / Q000378:metabolism*
D010472:Perchlorates