[Detection of herpes simplex virus by DNA-DNA hybridization method].
Eight recombinant clones were obtained by insertion of BamHI fragments of herpes simplex type I viral DNA into a vector plasmid pUC19o. Of the obtained clones 5 were found to hybridize with herpes simplex type I and 2 viral DNA while 3 clones revealed a positive reaction with the Vero cells DNA. A constructed DNA-probe possessing the highest level of activity was selected for further studies. The probe is a BamHI fragment of herpes simplex type I viral DNA labelled with 32P dTTP. Probe sensitivity in blot hybridization is 10 pg for identification of type I viral DNA and 50 pg for type 2 viral DNA. The DNAs of cytomegalovirus and herpes zoster virus do not show positive signals with the probe. The increased sensitivity of the used dot hybridization as compared with biological or IEA antigen identification of the virus was confirmed with the clinical material from 59 patients with the different clinical manifestations of the herpes viral infection.
Diorditsa
S V
SV
Zaĭtsev
I Z
IZ
Mal'tseva
N N
NN
Ebralidze
L K
LK
D000818:Animals; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism; D005814:Genes, Viral; D006561:Herpes Simplex / Q000175:diagnosis; D006562:Herpes Zoster / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics / Q000302:isolation & purification*; D014709:Vero Cells
D015342:DNA Probes; D004279:DNA, Viral
Vyiavlenie virusa gerpesa prostogo metodom DNK-DNK-gibridizatsii.