Monocyte responses to sulfatide from Mycobacterium tuberculosis: inhibition of priming for enhanced release of superoxide, associated with increased secretion of interleukin-1 and tumor necrosis factor alpha, and altered protein phosphorylation.
In monocytes, sulfatide, a lipid from Mycobacterium tuberculosis, blocked priming for enhanced release of superoxide (O2-) by the macrophage activating factors lipopolysaccharide, gamma interferon, interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha (TNF-alpha), and muramyl dipeptide. Sulfatide, in the presence of lipopolysaccharide, also caused increased secretion of IL-1 beta and TNF-alpha into monocyte culture medium. Sulfatide altered the pattern of phosphorylation of monocyte proteins. Cell lysates prepared from monocytes treated with sulfatide showed decreased activity of protein kinase C, but sulfatide did not directly inhibit protein kinase C activity when added to lysates. A known inhibitor of protein kinase C, staurosporine, also inhibited O2- release and caused increased secretion of IL-1 beta. Thus, sulfatide appeared to indirectly affect protein kinase C, implicating protein kinase C as part of the mechanism of priming. Because sulfatide blocked priming for enhanced release of O2-, which could interfere with monocyte bactericidal activity, while causing enhanced secretion of IL-1 beta and TNF-alpha, which could promote formation of granulomata, sulfatide might be an important factor in the pathogenesis of M. tuberculosis.
Brozna
J P
JP
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198.
Horan
M
M
Rademacher
J M
JM
Pabst
K M
KM
Pabst
M J
MJ
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010766:Phosphorylation; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D013433:Sulfoglycosphingolipids / Q000276:immunology*; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007375:Interleukin-1; D011506:Proteins; D013433:Sulfoglycosphingolipids; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate