RNA-RNA and RNA-protein interactions in 30 S ribosomal subunits. Association of 16 S rRNA fragments in the presence of ribosomal proteins.
The E. coli 16 S rRNA with single-site breaks centered at position 777 or 785 was obtained by RNase H site-specific cleavage of rRNA. Spontaneous dissociation of the cleaved 16 S rRNA into fragments occurred under 'native' conditions. The reassociation of the 16 S rRNA fragments was possible only in the presence of ribosomal proteins. The combination of S4 and S16(S17) ribosomal proteins interacting mainly with the 5'-end domain of 16 S rRNA was sufficient for reassociation of the fragments. The 30 S subunits with fragmented RNA at ca. 777 region retained some poly(U)-directed protein synthetic activity.
Afonina
E I
EI
A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University USSR.
Chichkova
N V
NV
Bogdanov
A A
AA
D001482:Base Composition; D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D004722:Endoribonucleases; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D010455:Peptides; D011072:Poly U / Q000235:genetics; D014176:Protein Biosynthesis; D012336:RNA, Ribosomal, 16S / Q000378:metabolism*; D016914:Ribonuclease H; D012261:Ribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D010455:Peptides; D012336:RNA, Ribosomal, 16S; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins; D011072:Poly U; C012001:polyphenylalanine; D004722:Endoribonucleases; D016914:Ribonuclease H