N-fatty acyl compounds inhibit myristoyl acylation of pp60v-src and reduce tumorigenicity of Rous sarcoma virus-infected cells.
Prevention of NH2-terminal myristoylation of pp60v-src was determined with N-fatty acyl glycinal derivatives. Of all the compounds tested, N-myristoyl and N-lauroyl glycinal diethylacetal, N-myristoyl glycyl glycinal diethylacetal, and N-myristoyl-4-aminobutyl aldehyde diethylacetal strongly inhibited myristoylation of pp60v-src; but N-myristoyl diglycyl, N-myristoyl triglycyl, N-decanoyl glycinal diethylacetal, and N-palmitoyl glycinal diethylacetal did not. N-Myristoyl glycinal diethylacetal (25 or 50 microM) suppressed both morphological transformation and colony formation of Rous sarcoma virus-infected chick embryo fibroblasts.
Shoji
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Tsujita
R
R
Hamuro
T
T
Funakoshi
T
T
Kubota
Y
Y
D000215:Acylation / Q000187:drug effects; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000502:physiology*; D015197:Carcinogenicity Tests; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002472:Cell Transformation, Viral / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D005227:Fatty Acids / Q000494:pharmacology*; D005347:Fibroblasts; D015151:Immunoblotting; D008856:Microscopy, Fluorescence; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D014410:Tumor Stem Cell Assay
D005227:Fatty Acids; D009227:Myristic Acids; D019814:Myristic Acid; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src)