[Renal tubular acidosis in primary Sjogren's syndrome: study on the immunology and ultrastructure of EBV].
The related antigens of EBV were examined by McAB against Epstein-Barr virus EAp 138. The related antibody of serous EBV VCA and EBNA of Sjogren's syndrome (SS) patients was examined by indirect immunofluorescence and immunoenzyme methods. EBV was found in the tubular epithelial cells by EM and the relationship between EBV and SS was studied. The results showed that (1) Around the nuclei and basement membrane of the proximal tubular epithelial cells there were positively reacting granules but not in the control group. (2) The related antibody of serous EBV VCA and EBNA showed positive reaction, and the highest titre was VCA-IgA 1:80, VCA-IgM 1:40, VCA-IgG 1:320 and EBNA-IgG 1:320 respectively. In addition, mature granules were found in the cytoplasm of the renal tubular epithelial cells.
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Z G
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Department of Pathology, Peking union Medical College Hospital.
D000141:Acidosis, Renal Tubular / Q000150:complications / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000276:immunology; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007684:Kidney Tubules / Q000382:microbiology / Q000648:ultrastructure; D008875:Middle Aged; D012859:Sjogren's Syndrome / Q000150:complications / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; C041788:Epstein-Barr viral capsid antigen; C033305:Epstein-Barr virus early antigen