Variations in [3H]ouabain binding of porcine skeletal muscle associated with feeding.
The maximal ouabain binding capacity (Bmax) of longissimus dorsi muscle has been determined over a 24 h period in young pigs living at 12 degrees C. Animals were fed once a day and Bmax was estimated at 4, 8, 12 or 24 h after a large meal. Mean values of Bmax +/- S.E.M. (pmol ouabain/g wet wt muscle) of 548 +/- 78 and 556 +/- 57 at 4 and 24 h after feeding were significantly greater than those of 383 +/- 24 and 393 +/- 30 at 8 and 12 h after feeding (P less than 0.02). The extent to which these differences represent apparent or real changes in numbers of ouabain binding sites and, hence, in Na+,K(+)-ATPase concentration is discussed.
Dauncey
M J
MJ
Department of Molecular and Cellular Physiology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge.
Burton
K A
KA
Morovat
A
A
D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D001665:Binding Sites / Q000502:physiology; D003080:Cold Temperature; D004435:Eating / Q000502:physiology*; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010042:Ouabain / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013552:Swine; D013696:Temperature
D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase