Phosphoinositide hydrolysis linked 5-HT2 receptors in fibroblasts from choroid plexus.
A serotonin (5-HT)-mediated phosphoinositide hydrolysis response was characterized in fibroblasts cultured from rabbit choroid plexus. 5-HT elicited a maximum 8-fold increase in [3H]inositol-phosphate ([3H]IP) formation, while the partial agonists, (+)-lysergic acid diethylamide and (-)-1-(4-bromo-2,5-dimethyoxyphenyl)-2-aminopropane caused 2- and 5-fold increases, respectively. Mianserin, ketanserin, and spiperone were equipotent at blocking the 5-HT-mediated response. Thus, agonist and antagonist profiles indicate interactions with 5-HT2 receptors.
Barker
E L
EL
Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232.
Burris
K D
KD
Sanders-Bush
E
E
D004290:DOM 2,5-Dimethoxy-4-Methylamphetamine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002831:Choroid Plexus / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006220:Haloperidol / Q000494:pharmacology; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000378:metabolism*; D007650:Ketanserin / Q000494:pharmacology; D008238:Lysergic Acid Diethylamide / Q000494:pharmacology*; D008297:Male; D008803:Mianserin / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D011224:Prazosin / Q000494:pharmacology; D011817:Rabbits; D011985:Receptors, Serotonin / Q000187:drug effects / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology*; D013134:Spiperone / Q000494:pharmacology
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D011985:Receptors, Serotonin; D004290:DOM 2,5-Dimethoxy-4-Methylamphetamine; D008803:Mianserin; C003943:2,5-dimethoxy-4-bromoamphetamine; D012701:Serotonin; D013134:Spiperone; D008238:Lysergic Acid Diethylamide; D007650:Ketanserin; D006220:Haloperidol; D011224:Prazosin