[Image analytic DNA cytometry--a possibility for assessment of prognosis after resection of ductal pancreatic cancers?].
In this study we analysed DNA-ploidy as a potential prognostic parameter in ductal pancreatic carcinoma. Paraffin embedded histological material, obtained by resection from 34 patients with a ductal pancreatic carcinoma, was selected for analysis. Tumor areas within the paraffin embedded material were identified by HE-stained reference sections. One 50 microns section was dewaxed, rehydrated and mechanically and enzymatically prepared to form a suspension of 10000 cells/ml. One milliliter of the suspension, which contained bare nuclei with small rests of cytoplasma, was centrifuged on glass slides. The fixed nuclei were air-dried and stained by Feulgen SITS technique, which allows for the quantitative measurement of DNA. The DNA analysis was carried out with a computer-controlled single-cell cytophotometry. In contrast to using flow cytometry, only the tumor cells were measured by image-cytometry. Overlapping nuclei, dirt and other artifacts as well as inflammatory cells were efficiently eliminated. With DNA image-cytometry, we could differentiate between hypotriploid, triploid, hypertriploid and tetraploid tumors. The DNA-content provided the strongest influence on prognosis in the multivariate analysis.
Böttger
T
T
Klinik und Poliklinik für Allgemein- und Abdominalchirurgie, Johannes-Gutenberg-Universität Mainz.
Störkel
S
S
Stöckle
M
M
Gerber
B
B
Junginger
T
T
D002285:Carcinoma, Intraductal, Noninfiltrating / Q000401:mortality / Q000473:pathology* / Q000601:surgery*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation*; D009367:Neoplasm Staging; D010179:Pancreas / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000401:mortality / Q000473:pathology* / Q000601:surgery*; D011003:Ploidies; D011183:Postoperative Complications / Q000401:mortality; D015996:Survival Rate
D004273:DNA, Neoplasm
Bildanalytische DNS-Cytometrie--eine Möglichkeit zur Prognosebeurteilung nach Resektion von duktalen Pankreascarcinomen?