title: Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs.
abstract: A lambda gt11 cDNA library constructed from human tracheo-bronchial mucosa was screened with a polyclonal antiserum raised to chemically deglycosylated pronase glycopeptides from human bronchial mucins. Out of 20 positives clones, one partial cDNA clone was isolated and allowed to map a novel human tracheo-bronchial mucin gene. It contains 48 nucleotide tandem repeats quite perfectly identical which encodes a protein containing about 50% of hydroxy amino-acids. This clone hybridized to polydisperse messages produced by human tracheo-bronchial and human colonic mucosae. The gene (proposed name MUC 4) from which cDNA is derived maps to chromosome 3.
authors.affiliation: Unité 16 INSERM, Lille, France.
authors.forename: N
authors.initials: N
authors.lastname: Porchet
authors.forename: V C
authors.initials: VC
authors.lastname: Nguyen
authors.forename: J
authors.initials: J
authors.lastname: Dufosse
authors.forename: J P
authors.initials: JP
authors.lastname: Audie
authors.forename: V
authors.initials: V
authors.lastname: Guyonnet-Duperat
authors.forename: M S
authors.initials: MS
authors.lastname: Gross
authors.forename: C
authors.initials: C
authors.lastname: Denis
authors.forename: P
authors.initials: P
authors.lastname: Degand
authors.forename: A
authors.initials: A
authors.lastname: Bernheim
authors.forename: J P
authors.initials: JP
authors.lastname: Aubert
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001980:Bronchi / Q000502:physiology; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000235:genetics; D014132:Trachea / Q000502:physiology
chemical_list: D009077:Mucins; D012333:RNA, Messenger; D004247:DNA