Chemical structure of the carbohydrate backbone of Vibrio parahaemolyticus serotype 012 lipopolysaccharide.
The chemical structure of the saccharide portion of Vibrio parahaemolyticus serotype 012 lipopolysaccharide was studied. Using chemical degradation and modification, as well as methylation analysis in combination with GLC-MS, laser-desorption mass spectrometry and 1H-NMR and 13C-NMR spectroscopy, the carbohydrate backbone of the lipopolysaccharide was characterized as a branched decasaccharide with the following structure: (formula; see text) In the native lipopolysaccharide two additional phosphate groups are present and 3-deoxy-D-threo-hexulosonic acid and D-galacturonic acid are bound via acid-labile linkages.
Kondo
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S
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Federal Republic of Germany.
Zähringer
U
U
Seydel
U
U
Sinnwell
V
V
Hisatsune
K
K
Rietschel
E T
ET
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002857:Chromium; D017608:Chromium Compounds; D008401:Gas Chromatography-Mass Spectrometry; D006603:Hexuronic Acids / Q000032:analysis; D007834:Lasers; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010504:Periodic Acid; D010710:Phosphates / Q000032:analysis; D013400:Sugar Acids / Q000032:analysis; D014736:Vibrio parahaemolyticus / Q000737:chemistry*
D017608:Chromium Compounds; D006603:Hexuronic Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D010710:Phosphates; D013400:Sugar Acids; D002857:Chromium; D010504:Periodic Acid; C002532:2-keto-3-deoxyoctonate; C007819:galacturonic acid; C028801:chromium trioxide