Promiscuity of GABAA-receptor beta 3 subunits as demonstrated by their presence in alpha 1, alpha 2 and alpha 3 subunit-containing receptor subpopulations.
Polyclonal antibodies were raised in rabbits against the GABAA-receptor beta 3 subunit peptide sequence, KQSMPREGHGRHMDR-NH2 coupled to keyhole limpet haemocyanin. These anti-beta 3 379-393 antibodies immunoprecipitated in a dose-dependent manner specific benzodiazepine agonist binding sites from Na+ deoxycholate extracts of bovine cerebral cortex. In immunoblots, anti-beta 3 379-393 antibodies recognised two species with Mr 59,900 and Mr 57,200 in all preparations tested, which included crude detergent-solubilised, benzodiazepine affinity chromatography-purified receptor, anti-alpha 1 324-341 antibody, anti-Cys alpha 2 414-424 antibody and anti-Cys alpha 3 454-467 antibody immunoaffinity-purified GABAA-receptor subpopulations. These results provide evidence for the ubiquity and promiscuity of the GABAA-receptor beta 3 subunit.
Pollard
S
S
Department of Pharmaceutical Chemistry, School of Pharmacy, London, UK.
Duggan
M J
MJ
Stephenson
F A
FA
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001665:Binding Sites; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D005445:Flunitrazepam / Q000378:metabolism; D015151:Immunoblotting; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D011963:Receptors, GABA-A / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism
D000906:Antibodies; D046911:Macromolecular Substances; D010455:Peptides; D011963:Receptors, GABA-A; D005445:Flunitrazepam