Specific cleavage of chicken alpha A-globin and human c-Ha-ras genes by two molecular forms of calf thymus topoisomerase I.
Two molecular forms of topoisomerase I differing in size and sensitivity to camptothecin were isolated from calf thymus. Mapping of topo I cleavage sites of the cloned chicken alpha A-globin and human c-Ha-ras genes was carried out. Camptothecin was shown to affect site specificity of the topoisomerases.
Bronstein
I B
IB
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Gromova
I I
II
Razin
S V
SV
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002166:Camptothecin / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D004247:DNA / Q000737:chemistry; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D011905:Genes, ras; D005914:Globins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D013950:Thymus Gland / Q000201:enzymology*
D005914:Globins; D004247:DNA; D004264:DNA Topoisomerases, Type I; D002166:Camptothecin