Transposon mutagenesis in Staphylococcus epidermidis using the Enterococcus faecalis transposon Tn917.
We transformed a clinical Staphylococcus epidermidis isolate with the Enterococcus faecalis transposon Tn917-carrying plasmid pTV1. Loss of plasmid replication was observed at 47 degrees C. Tn917 transposes efficiently and apparently randomly. The transposition frequency could be stimulated by erythromycin. Transposon mutagenesis in S. epidermidis provides a means for genetic study of the various virulence factors of this pathogen.
Grueter
L
L
Institut für Medizinische Mikrobiologie und Immunologie, Universität Hamburg, F.R.G.
Koenig
O
O
Laufs
R
R
D015139:Blotting, Southern; D004251:DNA Transposable Elements / Q000235:genetics*; D013293:Enterococcus faecalis / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology; D016254:Mutagenesis, Insertional / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics*; D013696:Temperature; D014169:Transformation, Bacterial / Q000187:drug effects / Q000235:genetics*
D004251:DNA Transposable Elements; D004917:Erythromycin