Molecular cloning and sequencing of cDNA encoding the phosphatidylinositol kinase from rat brain.
A complementary DNA (cDNA) clone that encodes phosphatidylinositol 4-kinase (PI 4-kinase) was isolated from a rat brain cDNA library. The deduced amino acid sequence of 697 residues revealed that the protein contains two putative transmembrane sequences and that the N-terminal part of the protein has several sequences representing potential phosphorylation sites for cAMP- and calmodulin-dependent kinase. The C-terminal region is probably a phosphotransferase domain homologous to the kinase region of protein kinase family proteins. Specific antibody against the protein expressed in Escherichia coli successfully immunoprecipitated rat brain PI 4-kinase. The messenger RNA for PI 4-kinase was found predominantly in brain and rat neural cell lines. This PI kinase may play a specific role in neural signal transduction.
Yamakawa
A
A
Department of Pharmacology, Tokyo Metropolitan Institute of Gerontology, Japan.
Nishizawa
M
M
Fujiwara
K T
KT
Kawai
S
S
Kawasaki
H
H
Suzuki
K
K
Takenawa
T
T
D019870:1-Phosphatidylinositol 4-Kinase; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011233:Precipitin Tests; D011487:Protein Conformation; D051381:Rats; D015183:Restriction Mapping
D004247:DNA; D010770:Phosphotransferases; D019870:1-Phosphatidylinositol 4-Kinase