The primary structure of rubrerythrin, a protein with inorganic pyrophosphatase activity from Desulfovibrio vulgaris. Comparison with hemerythrin and rubredoxin.
The complete polypeptide chain of rubrerythrin from the sulfate reducing bacterium Desulfovibrio vulgaris, strain Hildenborough NCIB 8303, was found by protein chemical techniques to consist of 191 residues and to have the amino acid sequence [sequence: see text] The C-terminal part of the protein (position 153----191) shows the typical sequence features of rubredoxin, a protein with a nonheme iron center also present in the same and other Desulfovibrio species. Based on the known three-dimensional structure of D. desulfuricans rubredoxin, we propose that the C-terminal part of rubrerythrin is folded in a similar way and suggest that the deletion of the extra 10 residues is compatible with the same basic rubredoxin-fold. After characterization of the C-terminal region, and in contrast to what could be expected from previously published spectroscopic analyses, the N-terminal region 1-152 of rubrerythrin appears to have no sequence similarity with the eukaryotic protein hemerythrin which is known to contain a binuclear iron center bound by 5 histidine ligands. However, the N-terminal region of rubrerythrin does contain 5 histidine residues but they are differently spaced along the peptide chain. We suggest that at least one of the 3 histidine residues located in the rubredoxin-like center of rubrerythrin may be liganded to one iron atom of the hemerythrin-like center. This paper is the first sequence report of a protein with pyrophosphatase activity although the physiological substrate for the rubrerythrin may be not inorganic pyrophosphate.
Van Beeumen
J J
JJ
Laboratory of Microbiology, State University of Ghent, Belgium.
Van Driessche
G
G
Liu
M Y
MY
LeGall
J
J
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D016969:Desulfovibrio vulgaris / Q000201:enzymology*; D005288:Ferredoxins / Q000737:chemistry*; D006422:Hemerythrin / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry; D011755:Pyrophosphatases / Q000737:chemistry*; D012416:Rubredoxins / Q000737:chemistry; D016415:Sequence Alignment
D001426:Bacterial Proteins; D005288:Ferredoxins; D006422:Hemerythrin; D010455:Peptides; D012416:Rubredoxins; C055857:rubrerythrins; D011755:Pyrophosphatases