Determination of the superoxide dismutase-like activity of cimetidine-Cu(II) complexes.
Using the direct method of pulse radiolysis to determine the superoxide dismutase like activity of copper(II) cimetidine complexes, it was found that the reaction rate constant with O2-, kcat, was (8.5 +/- 0.5) x 10(8) M-1s-1 independent of the cimetidine concentrations present in excess of 50-200 microM over the metal. The results suggest that either the 1:1 ligand to metal complex does not catalyze O2- dismutation at a comparable rate to that of the 2:1 complex, or that the stability constant of the last species is much higher than that determined earlier by Kimura et al., and only the 2:1 species is present in the solutions. With the indirect methods of cytochrome c and NBT for determining the ability of these complexes to catalyze O2 dismutation, these compounds exhibited a much lower SOD activity, and kcat was determined to be (5.0 +/- 0.3) x 10(6) and (7.6 +/- 0.4) x 10(7) M-1s-1, respectively using the two assays.
Goldstein
S
S
Department of Physical Chemistry, Hebrew University of Jerusalem, Israel.
Czapski
G
G
D002927:Cimetidine / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D009942:Organometallic Compounds / Q000378:metabolism*; D010084:Oxidation-Reduction; D011675:Pulse Radiolysis; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*
D003574:Cytochrome c Group; D009942:Organometallic Compounds; C054063:copper(II)-cimetidine complex; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D003300:Copper; D002927:Cimetidine; D013482:Superoxide Dismutase