title: Chromatin and cell surface receptors mediate melanoma cell growth response to nerve growth factor.
abstract: Growth response to nerve growth factor (NGF) was tested in the primary melanoma cell line WM 164, which expressed a low level of NGF cell-surface receptor, and in WM 164 cells transfected with cDNA for the cell-surface receptor (TrWM 164 cells), which expressed a higher level of the cell-surface receptor. Neither cell line expressed the chromatin receptor for NGF or internalized NGF. Both cell lines were stimulated to growth by NGF. After 10 d of exposure to NGF, a 230,000 Mr chromatin protein (receptor) was induced in both cell lines; as a result, NGF bound to the chromatin, and ribosomal RNA synthesis and cell proliferation were inhibited. We suggest that the cell-surface and chromatin receptors each mediate a different function of NGF.
authors.affiliation: Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
authors.forename: E M
authors.initials: EM
authors.lastname: Rakowicz-Szulczynska
authors.forename: U
authors.initials: U
authors.lastname: Reddy
authors.forename: A
authors.initials: A
authors.lastname: Vorbrodt
authors.forename: D
authors.initials: D
authors.lastname: Herlyn
authors.forename: H
authors.initials: H
authors.lastname: Koprowski
mesh_terms: D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D008546:Melanoma, Experimental / Q000473:pathology* / Q000503:physiopathology; D009414:Nerve Growth Factors / Q000378:metabolism / Q000494:pharmacology*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017475:Receptors, Nerve Growth Factor; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D009414:Nerve Growth Factors; D012335:RNA, Ribosomal; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor