Beta-adrenoceptor density on mononuclear leukocytes and right atrial myocardium in infants and children with congenital heart disease.
Sympathetic regulation of myocardial performance has been shown to be altered in congestive heart failure. Right atrial tissue of children with severe acyanotic and cyanotic congenital heart disease (CHD) showed a significantly lower beta-receptor density than that of children with less severe defects. Since mononuclear leukocytes (MNL) contain a homogeneous population of beta 2-adrenoceptors which have similar properties to those of cardiac beta 2-adrenoceptors, they are frequently used for studying the beta-adrenergic system. In a group of 37 children with CHD of different types and severity who underwent cardiac surgery, we compared the MNL beta-adrenoceptor density to the type and severity of CHD and looked for a possible relationship to plasma catecholamine levels and to the right atrial beta-adrenoceptor density. Membranes of MNL and myocardial cells were radiolabeled with (-)3-[125I]Iodocyanopindolol [( 125I]ICYP). A significantly higher beta-adrenoceptor density on MNL was found in patients with moderate acyanotic CHD (group I) than in those with severe acyanotic (group II) and cyanotic CHD (group III). Patients of group I showed approximately 50% higher myocardial beta-receptor density than those of groups II and III. ICI 118.551-[125I]ICYP competition studies revealed that in groups II and III significantly lower proportions and densities of beta 1-receptors were found compared to group I. Noradrenaline (NA) plasma levels in group II and group III were significantly higher than those in group I. The adrenaline plasma levels were found to be very high in all children with CHD.(ABSTRACT TRUNCATED AT 250 WORDS)
Kozlik
R
R
Kinderpoliklinik der Ludwig-Maximilians-Universität, München.
Kramer
H H
HH
Wicht
H
H
Bircks
W
W
Reinhardt
D
D
D001667:Binding, Competitive / Q000502:physiology; D002648:Child; D002675:Child, Preschool; D015536:Down-Regulation / Q000502:physiology*; D004837:Epinephrine / Q000097:blood; D005260:Female; D006325:Heart Atria / Q000503:physiopathology*; D006330:Heart Defects, Congenital / Q000503:physiopathology*; D006333:Heart Failure / Q000503:physiopathology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D020120:Iodocyanopindolol; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008297:Male; D009638:Norepinephrine / Q000097:blood*; D010869:Pindolol / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D011869:Radioligand Assay; D011943:Receptors, Adrenergic, beta / Q000502:physiology*
D011943:Receptors, Adrenergic, beta; D020120:Iodocyanopindolol; D010869:Pindolol; D009638:Norepinephrine; D004837:Epinephrine