title: Protein phosphatase 2A from Xenopus oocytes. Characterization during meiotic cell division.
abstract: A polyclonal antibody was raised against bacterially produced catalytic alpha subunit of protein phosphatase 2A (PP2AC) cloned from Xenopus ovarian library. The amount of PP2AC in Xenopus oocytes determined by Western blot analysis was 1 ng/microgram of cytosolic protein. The antibody depleted PP2AC from oocyte extracts in association with 6 components (40, 62, 65, 80, 85 and 90 kDa). Prophase- and metaphase-arrested oocytes contained identical amounts of PP2AC. Metaphase oocytes showed one specific change in the 62 kDa protein associated with PP2AC.
authors.affiliation: Laboratoire de Physiologie de la Reproduction, INRA, URA CNRS 1449, Université Pierre et Marie Curie, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Cormier
authors.forename: H B
authors.initials: HB
authors.lastname: Osborne
authors.forename: T
authors.initials: T
authors.lastname: Bassez
authors.forename: R
authors.initials: R
authors.lastname: Poulhe
authors.forename: R
authors.initials: R
authors.lastname: Bellé
authors.forename: O
authors.initials: O
authors.lastname: Mulner-Lorillon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002455:Cell Division; D004247:DNA / Q000235:genetics; D005260:Female; D015723:Gene Library; D008540:Meiosis; D008969:Molecular Sequence Data; D009865:Oocytes / Q000166:cytology / Q000201:enzymology*; D010053:Ovary / Q000201:enzymology; D010749:Phosphoprotein Phosphatases / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D054648:Protein Phosphatase 2; D015183:Restriction Mapping; D014981:Xenopus
chemical_list: D004247:DNA; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2