Endogenous cytochrome c and cytochrome oxidase in rat and mouse kidney: light microscopic histochemical study.
Activity of cytochrome c oxidase and the level of endogenous cytochrome c were investigated light microscopically in adult rat and mouse kidney by incubating unfixed frozen sections with diaminobenzidine (DAB) in the absence or presence of exogenous cytochrome c. The results suggest that DAB staining intensity mainly reflects the local density of mitochondria and only occasionally visualizes the differences in cytochrome oxidase activity and/or endogenous cytochrome c content. Most intense reaction was observed in proximal and distal tubules both in rat and mouse. Finer differentiation of reactivity in particular nephron segments and interspecies differences between rat and mouse kidney are also described.
Furgał
A
A
Department of Histology, Copernicus Medical Academy, Kraków, Poland.
Litwin
J A
JA
D015100:3,3'-Diaminobenzidine / Q000378:metabolism*; D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011919:Rats, Inbred Strains
D003574:Cytochrome c Group; D015100:3,3'-Diaminobenzidine; D003576:Electron Transport Complex IV