title: Major alterations in the nucleotide structure of DNA in cancer of the female breast.
abstract: DNA of invasive ductal carcinomas from five women was analyzed for structural alterations in the purine nucleotides using gas chromatography-mass spectrometry with selected ion monitoring. The results were compared to those for a normal DNA control. The carcinoma DNA showed dramatically higher concentrations of the base modifications 8-hydroxy-guanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine, and 8-hydroxyadenine. For example, the concentration of total identified base modifications represented a more than 9-fold increase over the control value. Base modifications of this type, which arise from radical-induced hydroxylation and cleavage reactions of the purine ring, likely play a major role in initiation and probably contribute to the further transformation of neoplastic cells in cancer of the female breast.
authors.affiliation: Environmental Biochemistry Program, Pacific Northwest Research Foundation, Seattle, Washington 98122.
authors.forename: D C
authors.initials: DC
authors.lastname: Malins
authors.forename: R
authors.initials: R
authors.lastname: Haimanot
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000032:analysis; D001943:Breast Neoplasms / Q000235:genetics*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002849:Chromatography, Gas; D004247:DNA / Q000032:analysis*; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D006900:Hydroxylation; D013058:Mass Spectrometry; D011743:Pyrimidines / Q000032:analysis
chemical_list: D011743:Pyrimidines; C071023:2,6-diamino-4-hydroxy-5-formamidopyrimidine; C002992:8-hydroxyadenine; C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA; D000225:Adenine