title: On the blood-brain barrier to peptides: specific binding of atrial natriuretic peptide in vivo and in vitro.
abstract: Using the intracarotid bolus injection technique, a saturable binding of [125I]atrial natriuretic peptide (ANP) was found in 8 blood-brain barrier (BBB)-protected rat brain regions as well as in the pineal gland, choroid plexus, neurointermediate and anterior lobes of the pituitary, i.e. structures lacking a BBB. The presence of specific ANP binding on the BBB, here shown for the first time by an in vivo approach, was evidenced concomitantly in vitro by incubation of isolated microvessels. A single-class high affinity binding without regional differences was obtained with Kd = 0.23 nM and Bmax = 120 fmol/mg protein. From that a density of 1,400 binding sites per endothelial cell was calculated, thought to be localized predominantly in the luminal membranes. In the in vivo study, the portion of the extracted peptide that, under the conditions used, may have crossed the BBB by passive diffusion amounted to less than 0.4% of the labeled ANP administered. ANP itself did not change the tightness of the BBB to the non-diffusible reference molecule [14C]inulin. In the BBB-free areas, ANP enhanced the inulin space by nearly 50%.
authors.affiliation: Department of Cell Biology and Regulation, University of Leipzig, F.R.G.
authors.forename: A
authors.initials: A
authors.lastname: Ermisch
authors.forename: H J
authors.initials: HJ
authors.lastname: Rühle
authors.forename: R
authors.initials: R
authors.lastname: Kretzschmar
authors.forename: A
authors.initials: A
authors.lastname: Baethmann
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001812:Blood-Brain Barrier; D002560:Cerebrovascular Circulation; D005110:Extracellular Space / Q000378:metabolism; D007444:Inulin / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008833:Microcirculation / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D007444:Inulin; D017461:Receptors, Atrial Natriuretic Factor