Separation of human serum lipoproteins into three major classes by hydroxyapatite chromatography.
The separation of normolipidemic male serum lipoprotein fraction, prepared by ultracentrifugal flotation, was studied on hydroxyapatite columns. Potassium phosphate buffers in the pH range 5.6-7.4 were evaluated as eluents. The three main classes of the lipoproteins (high density, low density and very low density) can be separated on the Tiselius-type hydroxyapatite (Bio-Gel HTP DNA grade) column by elution with 75, 250 and 300 mM potassium phosphate buffer (pH 7.4), respectively.
Matsumoto
U
U
Division of Analytical Chemistry, Tokyo College of Pharmacy, Japan.
Nakayama
H
H
Shibusawa
Y
Y
Niimura
T
T
D002021:Buffers; D002853:Chromatography, Liquid / Q000379:methods*; D017886:Durapatite; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D010710:Phosphates; D011188:Potassium; D017680:Potassium Compounds; D014461:Ultracentrifugation
D002021:Buffers; D006882:Hydroxyapatites; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D010710:Phosphates; D017680:Potassium Compounds; D017886:Durapatite; C013216:potassium phosphate; D011188:Potassium