Some chemical and biological properties of the low molecular weight heparan sulfate alpha-idosane.
A low molecular weight heparan sulfate derivative, alpha-idosane was separated from a mixture of glycosaminoglycans extracted from porcine mucosa. Its molecular weight, sulfur, uronic acid and hexosamine contents, C-NMR spectrum and electrophoretic properties are reported in this paper. The pharmacological effects of a-idosane were investigated "ex vivo" in dogs and rats. At doses of 10-50 mg/kg p.o., a-idosane shows fibrinolytic activity but it is devoid of anticoagulant action. At the dose of 100 mg/kg p.o. a-idosane exertes a significant anti-inflammatory effect but is unable to protect the rats against arachidonate-induced sudden death.
Gervasi
G B
GB
Baldacci Research Laboratories, Pisa, Italy.
Bartoli
C
C
Farina
C
C
Catalani
R
R
Carpita
G
G
Pistelli
N
N
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000925:Anticoagulants; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000037:antagonists & inhibitors / Q000633:toxicity; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004285:Dogs; D005342:Fibrinolysis / Q000187:drug effects; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry* / Q000494:pharmacology; D006497:Heparan Sulfate / Q000737:chemistry* / Q000494:pharmacology; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000925:Anticoagulants; D016718:Arachidonic Acid; D001095:Arachidonic Acids; D006495:Heparin, Low-Molecular-Weight; D006497:Heparan Sulfate