[Effects of ethanol on acid secretion by isolated parietal cells from guinea pig].
We studied the effects of ethanol (0.1-10%) on acid secretion of parietal cell-rich fractions isolated from guinea pig gastric mucosa. Ethanol (0.1-3%) increased histamine-stimulated cAMP content, while over 1% ethanol decreased histamine-stimulated acid secretion. H+, K(+)-ATPase activity in microsomal fraction also decreased after treatment with 3% ethanol. Thus, ethanol may disturb the signalling process from cAMP to H+, K(+)-ATPase. On the other hand, carbachol-stimulated acid secretion was more sensitive to ethanol than that with histamine, and 0.1% ethanol suppressed the acid secretion. This effect was well correlated with the extent of the ethanol-induced increase of [Ca2+]i and with the attenuation of [Ca2+]i response following carbachol stimulation. The calcium response may be a primary target against ethanol in carbachol-dependent process. In conclusion, low-dose ethanol have multi-effects on these critical intermediary steps in acid secretion.
Niki
S
S
Department of Preventive Medicine, Kyoto Prefectural University of Medicine.
Nakamura
K
K
Rokutan
K
K
Saitoh
T
T
Aoike
A
A
Kawai
K
K
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D005744:Gastric Acid / Q000378:metabolism*; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D010295:Parietal Cells, Gastric / Q000187:drug effects* / Q000378:metabolism
D000431:Ethanol; D006632:Histamine; D002217:Carbachol; D000242:Cyclic AMP