title: Ontogeny of insulin-like growth factor I and insulin receptor kinase activity in rat liver.
abstract: IGF-I and insulin receptors possess tyrosine-kinase enzymatic activity considered to be essential for signal transduction and thereby mediating the putative effects of these hormones on fetal growth and development. We investigated the ontogeny of IGF-I and insulin receptor tyrosine-kinase activity in at least 3 separate membrane preparations from liver of rats at 21 day of embryonic life (21ED), 1 and 5 day of postnatal life (1PD and 5PD respectively) and adult. Receptors purified by wheat germ agglutinin chromatography (WGA) were exposed to graded concentrations of IGF-I or insulin, and tyrosine-kinase activity was measured by quantifying incorporation of 32P into the exogenous substrate poly[Glu,Tyr; 4:1]. IGF-I stimulated tyrosine-kinase solely at 1 PD as documented by a maximal increase of 346 +/- 167% over basal kinase activity with 6.6 nmol/L IGF-I. While the lack of response in adult animals could be explained by a striking decrease in receptors at that age, 125I-IGF-I binding and affinity labelling of the WGA preparations indicated substantial IGF-I receptors were present in the liver at each of the perinatal ages. Furthermore, this dissociation between IGF-I binding and the tyrosine-kinase activity of these IGF-I receptors could not be attributed to the presence/absence of IGF-I binding proteins as judged by affinity labelling. In contrast, insulin-stimulated tyrosine-kinase activity was observed at all ages tested although it appeared greatest at 1PD. We conclude that (i) expression of IGF-I tyrosine-kinase activity is linked to developmental events and differs from that found for the insulin receptor tyrosine-kinase activity, (ii) during the perinatal period there is an apparent dissociation between ligand binding by the IGF-I receptor and receptor tyrosine-kinase activity. These observations suggest modulation of IGF-I receptor tyrosine-kinase activity may be an important regulator of IGF-I action during the perinatal period.
authors.affiliation: Division of Endocrinology, Children's Hospital Medical Center, University of Cincinnati, OH 45229.
authors.forename: R K
authors.initials: RK
authors.lastname: Menon
authors.forename: S D
authors.initials: SD
authors.lastname: Chernausek
authors.forename: M A
authors.initials: MA
authors.lastname: Sperling
mesh_terms: D000818:Animals; D005260:Female; D005325:Fetal Organ Maturity / Q000502:physiology; D005333:Fetus / Q000502:physiology; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D008099:Liver / Q000196:embryology / Q000254:growth & development*; D008297:Male; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin; D014909:Wheat Germ Agglutinins
chemical_list: D014909:Wheat Germ Agglutinins; D007334:Insulin-Like Growth Factor I; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin