Molecular cloning of the ViaB region of Salmonella typhi.
The ViaB region required for Vi antigen production in Salmonella typhi was cloned. The plasmid pGBM124 containing a 14-kb S. typhi chromosomal DNA fragment conferred the ability to produce Vi antigen on Escherichia coli HB101 and ViaB-deleted S. typhi GIFU10007-3. Tn5 insertion analysis showed that the 14-kb DNA was split into three regions. Region 1 and region 2 are involved in the biosynthesis of Vi polysaccharide. Region 3 is involved in translocation of the Vi polysaccharide to the cell surface. Southern blot hybridization showed that regions 2 and 3 but not region 1, were considerably homologous to the DNA of Vi-positive Citrobacter freundii.
Hashimoto
Y
Y
Department of Microbiology, Gifu University School of Medicine, Japan.
Ezaki
T
T
Li
N
N
Yamamoto
H
H
D000942:Antigens, Bacterial / Q000235:genetics*; D016971:Citrobacter freundii / Q000235:genetics; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry; D005798:Genes, Bacterial; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011135:Polysaccharides, Bacterial; D015183:Restriction Mapping; D012485:Salmonella typhi / Q000235:genetics* / Q000276:immunology
D000942:Antigens, Bacterial; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial