Modified tetrazolium-based colorimetric method for determining the activities of anti-HIV compounds.
A modified tetrazolium-based colorimetric assay was used to determine the anti-HIV activities of ddAzThd, ddCyd, ddIno, and PFA. In this assay, poly-1-lysine-coated plates were used to attach the MT-2 cells to the bottom of the plates. A fixed amount of virus (50 TCID50) was used in each well. A modified version of the formula published by Pauwels et al. (1988) was used for calculating the percentage cell protection from virus infection. Using CC10/EC90 to calculate the selective indices, the decreasing order of selectivity against HIV-1 strain A87SF, was: ddAzThd greater than PFA greater than ddCyd greater than ddIno. Against HIV-1 strain A79SK-1 the decreasing order of selectivity was: PFA greater than ddIno greater than AzThd greater than ddCyd. The modified formula showed lack of anti-HIV activity for thymidine at non-toxic concentrations.
Ayisi
N K
NK
Noguchi Memorial Institute for Medical Research, University of Ghana, Legon.
Gupta
S V
SV
Qualtiere
L F
LF
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D003124:Colorimetry / Q000379:methods*; D016049:Didanosine / Q000494:pharmacology; D017245:Foscarnet; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D013778:Tetrazolium Salts; D013936:Thymidine / Q000494:pharmacology; D016047:Zalcitabine / Q000494:pharmacology; D015215:Zidovudine / Q000494:pharmacology
D000998:Antiviral Agents; D013778:Tetrazolium Salts; D017245:Foscarnet; D015215:Zidovudine; D016047:Zalcitabine; D016049:Didanosine; D010746:Phosphonoacetic Acid; D013936:Thymidine