title: Characterization of crystals of an Fab fragment of a murine monoclonal antibody.
abstract: The Fab fragment of an antibody, made against an E2-specific feline infectious peritonitis virus neutralizing antibody, has been crystallized in a form suitable for X-ray diffraction analysis from PEG 4000 using vapor diffusion methods. The Fab fragment crystals diffract to about 2.9 A resolution and are of triclinic space group P1. Unit cell dimensions, by which the reciprocal lattice can be indexed, are a = 57.16 A, b = 70.85 A, c = 75.81 A, alpha = 85.11 degrees, beta = 121.28 degrees and gamma = 116.33 degrees. There are two Fab fragments comprising the asymmetric unit of the crystals. The presence of a pseudo-mirror plane in the diffraction pattern suggests the presence of at least an approximate dyad axis relating the two Fab fragments within the asymmetric unit.
authors.affiliation: Department of Biochemistry, University of California, Riverside 92521.
authors.forename: N
authors.initials: N
authors.lastname: Ban
authors.forename: C
authors.initials: C
authors.lastname: Escobar
authors.forename: J
authors.initials: J
authors.lastname: Day
authors.forename: A
authors.initials: A
authors.lastname: Greenwood
authors.forename: S
authors.initials: S
authors.lastname: Larson
authors.forename: A
authors.initials: A
authors.lastname: McPherson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D016765:Coronavirus, Feline / Q000276:immunology*; D003460:Crystallization; D003461:Crystallography; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments