Biochemical characterization of herpes simplex virus type-1-immunostimulating complexes (ISCMOs): a multi-glycoprotein structure.
The preparation and characterization of an immunostimulating complex (ISCOM) preparation containing several HSV-1 glycoproteins, including the major glycoproteins B and D is described. The multi-glycoprotein HSV-1 ISCOM preparation was obtained from a gradient-purified aqueous HSV-1 antigen preparation following extraction from infected cells using a zwitterionic detergent. With polyclonal and monoclonal antibodies to HSV-1 glycoproteins in enzyme-linked immunosorbent assay, SDS-polyacrylamide gel electrophoresis and radioimmunoprecipitation techniques, the HSV-1 ISCOM preparation was shown to contain glycoproteins B, C, D, E, H and I, although further, additional proteins were also present. The DNA content of HSV-1 ISCOMs was determined using a 3H-thymidine labelling method. The protein and DNA contents of the HSV-1 ISCOM preparation are discussed with reference to the potentialities of the preparation as a vaccine for use in human beings.
Erturk
M
M
Department of Virology, University of Sheffield Medical School, UK.
Jennings
R
R
Phillpotts
R J
RJ
Potter
C W
CW
D004279:DNA, Viral / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D016882:ISCOMs / Q000032:analysis*; D018139:Simplexvirus / Q000737:chemistry*; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000302:isolation & purification; D014765:Viral Vaccines / Q000032:analysis*
D004279:DNA, Viral; D006023:Glycoproteins; D016882:ISCOMs; D014759:Viral Envelope Proteins; D014765:Viral Vaccines