Apical expression of herpes simplex virus type 2 glycoproteins in human neuroblastoma cells.
The expression of herpes simplex virus type 2 (HSV-2) glycoproteins on the surface of human neuroblastoma cells has been investigated using Millipore Millicell culture plate inserts. Utilizing a modified radioimmunoassay, we learned that glycoproteins B, C, D, E, and I were expressed predominantly on the apical membrane domain of the infected neuroblastoma cells. The unidirectional transport of HSV-2 glycoproteins was substantiated by the analysis of extracellular glycoproteins released from neuroblastoma cells. The results suggest that the evaluated HSV-2 glycoproteins were transported primarily to the apical plasma membrane domain of human neuroblastoma cells.
Nielsen
L N
LN
University of Alabama, Department of Pediatrics, Birmingham 35294.
Whitley
R J
RJ
Chatterjee
S
S
D002462:Cell Membrane / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology*; D015151:Immunoblotting; D011863:Radioimmunoassay; D018139:Simplexvirus / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000378:metabolism
D014759:Viral Envelope Proteins