title: Luteal enzymes of the luteinizing hormone and beta-adrenergic signal transduction pathways in hypophysectomized rabbits do not require pituitary hormone support.
abstract: Experiments were conducted to determine whether continuous pituitary hormone support is required for expression of the LH and beta-adrenergic cAMP signal transduction pathways in rabbit CL during pseudopregnancy. Parameters of the LH and catecholamine cAMP signal transduction pathways in CL of estrogen-treated hypophysectomized rabbits were compared to those of pituitary-intact rabbits. Results showed that each of the parameters of the LH and beta-adrenergic cAMP signal transduction pathways was retained in CL taken from estrogen-treated pseudopregnant rabbits that had been hypophysectomized for as long as 13 days at levels not significantly different from those of estrogen-treated pituitary-intact rabbits. These included luteal basal, and LH-, epinephrine-, and fluoride-stimulated adenylyl cyclase activities; total luteal cAMP levels; the number and affinity of cAMP-dependent protein kinase regulatory subunit cAMP binding sites; binding activity of the type I and type II regulatory subunits; and the amount of catalytic subunit protein of cAMP-dependent protein kinase. We conclude that expression of the proteins of the cAMP signal pathway for LH and beta-adrenergic hormones in CL of estrogen-treated rabbits does not require pituitary hormone support.
authors.affiliation: Department of Cell, Northwestern University Medical School, Chicago, Illinois 60611.
authors.forename: M
authors.initials: M
authors.lastname: Hunzicker-Dunn
authors.forename: A
authors.initials: A
authors.lastname: Chen
authors.forename: V
authors.initials: V
authors.lastname: Jackiw
authors.forename: J E
authors.initials: JE
authors.lastname: Gadsby
authors.forename: C H
authors.initials: CH
authors.lastname: Bill
authors.forename: A R
authors.initials: AR
authors.lastname: LaBarbera
authors.forename: J B
authors.initials: JB
authors.lastname: Miller
authors.forename: P L
authors.initials: PL
authors.lastname: Keyes
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000201:enzymology / Q000502:physiology*; D000242:Cyclic AMP / Q000502:physiology; D005260:Female; D007016:Hypophysectomy; D007986:Luteinizing Hormone / Q000502:physiology*; D010902:Pituitary Gland / Q000502:physiology; D010907:Pituitary Hormones / Q000502:physiology; D011555:Pseudopregnancy / Q000503:physiopathology; D011817:Rabbits; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D010907:Pituitary Hormones; D011943:Receptors, Adrenergic, beta; D007986:Luteinizing Hormone; D000242:Cyclic AMP