He-T family DNA sequences in the Y chromosome of Drosophila melanogaster share homology with the X-linked stellate genes.
The genome of Drosophila melanogaster contains a class of repetitive DNA sequences called the He-T family, which is unusual in being confined to telomeric and heterochromatic regions. The specific He-T fragment designated Dm665 was cloned in yeast by selection for an autonomously replicating sequence (ARS). Dm665 contains a restriction fragment length polymorphism (RFLP) that is specific to males and thus derives from the Y chromosome. Deletion mapping using X-Y translocations indicates that sequences homologous to Dm665 occur in at least one major cluster in each arm of the Y chromosome. Among 20 yeast artificial chromosome (YAC) clones containing Drosophila sequences homologous with Dm665, four clones derive from defined regions of the long arm of the Y and two from the short arm. The sequence of Dm665 is 2443 bp long, consists of 59% A + T, and contains no significant open reading frames or direct or inverted repeats. However, Dm665 contains a region of 650 bp that shares homology with portions of the X-linked locus Stellate.
Danilevskaya
O N
ON
Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110-1095.
Kurenova
E V
EV
Pavlova
M N
MN
Bebehov
D V
DV
Link
A J
AJ
Koga
A
A
Vellek
A
A
Hartl
D L
DL
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004247:DNA; D004330:Drosophila / Q000235:genetics*; D005260:Female; D015723:Gene Library; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome; D014998:Y Chromosome
D004247:DNA