Monoclonal antibody specific for BK virus large-T antigen allows discrimination among the different papovaviral large-T antigens.
The human papovaviruses BK virus (BKV) and JC virus (JCV) both encode a large-tumor (T) antigen which are highly homologous to each other as well as to simian virus 40 (SV40) large-T antigen. This high conservation of amino acid sequence has resulted in overlapping antigenicity such that immunological differentiation of the large-T antigens from the three different viruses has been difficult. Here we describe the generation and characterization of a new monoclonal antibody (BK-T.1) which is specific for BKV large-T antigen and which does not cross-react with the other papovaviral T antigens. We show that when BK-T.1 is used in conjunction with preexisting monoclonal antibodies against SV40 large-T antigen, it is possible to identify the origin of papovavirus T antigens.
Marshall
J
J
Laboratory of Cellular Regulation, Genzyme Corporation, Framingham, Massachusetts 01701.
Smith
A E
AE
Cheng
S H
SH
D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis*; D001739:BK Virus / Q000145:classification / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D027383:Papillomaviridae / Q000145:classification / Q000276:immunology*; D027382:Polyomaviridae; D013539:Simian virus 40 / Q000276:immunology
D000911:Antibodies, Monoclonal; D000952:Antigens, Polyomavirus Transforming