Analyses of polypeptides in the liver of a novel mutant (LEC rats) to hereditary hepatitis and hepatoma by two-dimensional gel electrophoresis: identification of P29/6.8 as carbonic anhydrase III and triosephosphate isomerase.
1. Total cellular proteins from the livers of 4-, 16- and 52-week-old hepatitis- and hepatoma-predisposed Long-Evans Cinnamon (LEC) rats were compared to those from the livers of the corresponding control rats [Long-Evans Agouti (LEA) rats] by two-dimensional gel electrophoresis. 2. A polypeptide, p50/7.2 (molecular weight x 10(-3)/isoelectric point) was only found in the LEC rats, and the p43/6.4 component was greater and the p51/6.8 component was less in the LEC rats than in the LEA rats during aging. 3. A polypeptide, p29/6.8, was dramatically greater in 4-week-old LEC rats than in 4-week-old LEA rats. 4. By sequencing and Western blotting analysis, the marked differences in the level of the p29/6.8 component were found to be due to carbonic anhydrase III.
Nagase
T
T
Department of Biochemistry, Osaka University Medical School, Japan.
Sugiyama
T
T
Kawata
S
S
Tarui
S
S
Deutsch
H F
HF
Taniguchi
N
N
D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002256:Carbonic Anhydrases / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000235:genetics / Q000662:veterinary*; D004198:Disease Susceptibility; D015180:Electrophoresis, Gel, Two-Dimensional; D006520:Hepatitis, Animal / Q000201:enzymology* / Q000235:genetics; D015151:Immunoblotting; D008099:Liver / Q000201:enzymology*; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics / Q000662:veterinary*; D008297:Male; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D051381:Rats; D011922:Rats, Mutant Strains; D012376:Rodent Diseases / Q000201:enzymology; D014305:Triose-Phosphate Isomerase / Q000378:metabolism
D010455:Peptides; D002256:Carbonic Anhydrases; D014305:Triose-Phosphate Isomerase