title: Congenital hypothyroidism in two cats due to defective organification: data suggesting loosely anchored thyroperoxidase.
abstract: Two cats with congenital hypothyroidism are described. In vivo discharge of accumulated labelled iodide by perchlorate administration revealed defective organification of iodide, which was complete in one cat and partial in the other. In the cat with the partial organification defect, thyroid tissue was obtained for biochemical studies. No membrane-bound peroxidase activity could be demonstrated. The activity was found in the 100,000 x g supernatant. It is suggested that the loose enzyme anchoring caused decreased availability of peroxidase and as a consequence reduced capacity for organic binding of trapped iodide.
authors.affiliation: Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
authors.forename: B E
authors.initials: BE
authors.lastname: Sjollema
authors.forename: M T
authors.initials: MT
authors.lastname: den Hartog
authors.forename: J J
authors.initials: JJ
authors.lastname: de Vijlder
authors.forename: J E
authors.initials: JE
authors.lastname: van Dijk
authors.forename: A
authors.initials: A
authors.lastname: Rijnberk
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000378:metabolism*; D002415:Cats; D003409:Congenital Hypothyroidism; D007037:Hypothyroidism / Q000378:metabolism / Q000662:veterinary*; D007275:Injections, Intravenous; D007453:Iodide Peroxidase / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D010472:Perchlorates / Q000494:pharmacology; D017670:Sodium Compounds; D013961:Thyroid Gland / Q000378:metabolism / Q000473:pathology
chemical_list: D007457:Iodine Radioisotopes; D010472:Perchlorates; D017670:Sodium Compounds; C031068:sodium perchlorate; D007453:Iodide Peroxidase