Immunohistochemistry of the pituitary pars distalis of the musk shrew, Suncus murinus.
Immunocharacteristics of the pituitary pars distalis cell types of the musk shrew, Suncus murinus, were studied by the unlabeled antibody enzyme technique, using peroxidase-antiperoxidase or avidin-biotin-peroxidase complex. The thyrotropin (TSH)-, gonadotropin (GTH)-, corticotropin (ACTH)-, prolactin (PRL)-, and growth hormone (GH)-secreting cells of the PD were identified on the basis of their immunoreactivity with different heterologous antisera. The TSH cells showed specific immunoreactivity with antisera against human (h) TSH beta and rat (r) TSH beta. Cells showing immunoreactivity with the antisera against hLH beta and ovine (o) LH beta were designated as GTH cells as no immunoreactivity was observed with antisera against hFSH beta and oFSH beta. The ACTH cells as well as the cells of the pars intermedia were revealed by anti-ACTH1-24 and anti-ACTH1-10 sera. Whereas the PRL cells were recognized by their immunoreactivity with antisera against hPRL and oPRL, the GH cells were identified with anti-hGH, anti-oGH, and anti-bovine (b) GH sera. TSH and GTH, TSH and ACTH, GTH and ACTH, ACTH and GH, ACTH and PRL, and GH and PRL cells were visualized in the same section using the dual immunoperoxidase technique. Comparison of the immunohistochemically identified cells with those described histochemically reveals several discrepancies, which expose the limitations of the latter techniques identifying adenohypophysial cells.
Naik
D R
DR
Department of Anatomy, Jikei University School of Medicine, Tokyo, Japan.
Shirasawa
N
N
Nogami
H
H
Ishikawa
H
H
D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis; D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000096:biosynthesis; D006065:Gonadotropins, Pituitary / Q000096:biosynthesis; D013006:Growth Hormone / Q000096:biosynthesis; D007150:Immunohistochemistry; D008297:Male; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D011388:Prolactin / Q000096:biosynthesis; D012788:Shrews / Q000378:metabolism*; D013972:Thyrotropin / Q000096:biosynthesis
D006065:Gonadotropins, Pituitary; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D013006:Growth Hormone