Inhibition of a cell surface protease after cisplatin chemotherapy.
The epithelial cells in squamous carcinoma and leukoplakia of the oral cavity possess the cell surface protease, guanidinobenzoatase (GB), in an active form. GB is closely similar to plasminogen activator, a protease associated with both transformed cells and tumour cells. The active centre of GB binds the fluorescent probe 9-aminoacridine (9-AA) enabling cells containing active GB to be visualised by fluorescent microscopy. It was observed that chemotherapy with cisplatin resulted in a marked decrease in cell surface GB activity and this decrease was due to the formation of an enzyme-inhibitor complex. One of the results of chemotherapy was shown to be the suppression of a cell surface protease which is known to be associated with migration and malignancy of cells in vivo.
Steven
F S
FS
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, UK.
Zoeller
J
J
Maier
H
H
Born
I A
IA
D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D002462:Cell Membrane / Q000201:enzymology*; D002945:Cisplatin / Q000627:therapeutic use*; D010450:Endopeptidases / Q000378:metabolism*; D004848:Epithelium / Q000201:enzymology / Q000473:pathology; D005557:Formaldehyde; D006801:Humans; D007700:Kinetics; D007971:Leukoplakia / Q000201:enzymology / Q000473:pathology; D008856:Microscopy, Fluorescence; D009061:Mouth Mucosa / Q000201:enzymology; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D011480:Protease Inhibitors; D014059:Tongue / Q000201:enzymology
D011480:Protease Inhibitors; D005557:Formaldehyde; D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases; D002945:Cisplatin