[The effect of serotonin on the functional activity of monocytes].
The treatment of human peripheral blood monocytes with serotonin at concentrations 10(-3) and 10(-5) M over 20 minutes decreases a zymosan-induced luminol-dependent chemiluminescence of cells, whereas a 5 minutes treatment with serotonin at the concentration of 10(-5) M increases the chemoluminescence. The correlated change in monocyte capacity of secreting hydrogen peroxide has been registered. Serotonin activates, to a little extent, the monocyte capacity of phagocytizing the opsonised sheep erythrocyte. The maximum increase (2-3 times) of intracellular cAMP content and the decrease in cytosol cAMP-binding capacity are registered after a 5 minutes incubation. The lowering of the share of irreversibly bound in vitro cAMP under the influence of serotonin may suggest a preferable binding of cyclic nucleotide in vivo by regulatory subunits of cAMP-dependent protein kinase I.
Tokmakov
A A
AA
Kykhova
M P
MP
Koshkina
O V
OV
Vasil'ev
V Iu
VIu
D002478:Cells, Cultured / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D000242:Cyclic AMP / Q000097:blood; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D008163:Luminescent Measurements; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009895:Opsonin Proteins / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D012701:Serotonin / Q000494:pharmacology*; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology
D009895:Opsonin Proteins; D012701:Serotonin; D015054:Zymosan; D006861:Hydrogen Peroxide; D000242:Cyclic AMP
Vliianie serotonina na funktsional'nuiu aktivnost' monotsitov.