Characterization of the Sendai virus V protein with an anti-peptide antiserum.
The Sendai virus V protein, which is a fusion of the P and V ORFs of the P gene, was characterized with antisera to a portion of the V ORF and compared to the P protein. The only property found in common with P is that V is also highly phosphorylated, and this is so even when these proteins are expressed independently of the other viral proteins. Otherwise, V was not found in virions, was not strongly associated with viral nucleocapsids like P, and anti-V had no effect on viral RNA synthesis in vitro under conditions where anti-P was highly inhibitory. The available evidence suggests that V may play a role in RNA synthesis, but it is not an essential one like that of the P protein.
Curran
J
J
Department of Microbiology, University of Geneva Medical School, Switzerland.
de Melo
M
M
Moyer
S
S
Kolakofsky
D
D
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010222:Parainfluenza Virus 1, Human / Q000235:genetics*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012261:Ribonucleoproteins / Q000235:genetics / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D010455:Peptides; D012333:RNA, Messenger; D012367:RNA, Viral; D012261:Ribonucleoproteins; D014764:Viral Proteins