The antiinflammatory effect of dopamine in alcoholic hemorrhagic pancreatitis in cats. Studies on the receptors and mechanisms of action.
Hemorrhagic pancreatitis was induced in cats by perfusing pancreatic enzymes through a pancreatic duct after the administration of intragastric ethanol. Dimethyl prostaglandin E2 was administered concurrently. In the first study, dopamine's antiinflammatory effect on the pancreas was determined in the presence of haloperidol, propranolol, or both. Next, dopamine's effects on blood flow in the normal and inflamed pancreas were compared using a hydrogen gas-clearance technique. In the final study, the effect of dopamine on fluorescein isothiocyanate-labeled dextran leakage from the pancreatic duct to portal venous blood was investigated. It was found that blockade of either dopamine or beta-adrenergic receptors reduced, and blockade of both receptors completely eliminated, the antiinflammatory effect. Dopamine had no effect on pancreatic blood flow in normal cats. In pancreatitis, although dopamine transiently reduced blood flow, after an hour flow had returned to normal. Dopamine reversed the leakage of fluorescein isothiocyanate-labeled dextran from the pancreatic duct caused by ethanol and by ethanol and prostaglandin E2. It was concluded that dopamine ameliorated pancreatitis by reducing pancreatic ductal and/or microvascular permeability rather than by altering pancreatic blood flow. The antiinflammatory effect was mediated by both dopamine and beta-adrenergic receptors.
Karanjia
N D
ND
Department of Surgery, Veterans Administration Medical Center, Sepulveda, California.
Widdison
A L
AL
Lutrin
F J
FJ
Chang
Y B
YB
Reber
H A
HA
D000208:Acute Disease; D000437:Alcoholism / Q000150:complications*; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D002415:Cats; D004298:Dopamine / Q000494:pharmacology / Q000627:therapeutic use*; D000431:Ethanol / Q000494:pharmacology; D006220:Haloperidol / Q000494:pharmacology; D006470:Hemorrhage / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D010179:Pancreas / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology; D010195:Pancreatitis / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D011433:Propranolol / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000276:immunology; D011954:Receptors, Dopamine / Q000187:drug effects / Q000276:immunology
D011458:Prostaglandins E; D011943:Receptors, Adrenergic, beta; D011954:Receptors, Dopamine; D000431:Ethanol; D011433:Propranolol; D006220:Haloperidol; D004298:Dopamine