title: Thomsen-Friedenreich (T)-active glycoproteins, blood group N antigen precursor glycoproteins and N antigen precursor glycoproteins with T activity from ascitic fluids of liver cancer patients.
abstract: 1. Five perchloric acid-soluble fractions (PASFs) obtained from ascitic fluids of three patients with primary hepatocellular carcinoma (HCC), a patient with liver metastatic carcinoma (LUC) from ureteral carcinoma and human normal serum (NS) were subjected to DEAE-cellulose column chromatography to separate seven glycoprotein fractions, respectively. 2. In this chromatography, two HCC-PASFs gave a Thomsen-Friedenreich (T)-active glycoprotein, respectively. 3. Other HCC-PASF gave a T-active glycoprotein, two blood group N antigen precursor glycoproteins and an N antigen precursor glycoprotein with T activity. 4. LUC-PASF gave two T-active glycoproteins and an N antigen precursor glycoprotein with T activity. 5. NS-PASF did not give these serologically active glycoproteins.
authors.affiliation: Department of Biochemistry, Tokyo College of Pharmacy, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Otsuka
authors.forename: S
authors.initials: S
authors.lastname: Nishida
authors.forename: H
authors.initials: H
authors.lastname: Yuasa
authors.forename: A
authors.initials: A
authors.lastname: Hara
authors.forename: T
authors.initials: T
authors.lastname: Shirai
authors.forename: S
authors.initials: S
authors.lastname: Ohkuma
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001202:Ascitic Fluid / Q000737:chemistry / Q000378:metabolism*; D001789:Blood Group Antigens; D006528:Carcinoma, Hepatocellular / Q000737:chemistry* / Q000556:secondary; D002848:Chromatography, DEAE-Cellulose; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007519:Isoantigens / Q000737:chemistry / Q000378:metabolism*; D008113:Liver Neoplasms / Q000737:chemistry* / Q000556:secondary; D010472:Perchlorates / Q000737:chemistry; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*; D014312:Trisaccharides / Q000737:chemistry / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000473:pathology
chemical_list: D000596:Amino Acids; D001789:Blood Group Antigens; D007519:Isoantigens; D010472:Perchlorates; D011498:Protein Precursors; C064068:Thomsen-Friedenreich antigen, cryptic; D014312:Trisaccharides