[The status of human papillomavirus 16 DNA in the tissues of human esophagus carcinoma].
DNAs from 24 cases of human esophagus carcinoma and 24 cases of corresponding para-carcinoma tissues were detected by dot hybridization and Southern hybridization techniques using human papillomavirus (HPV) 16 type DNA as probe. The results indicated that the positive rate of the HPV 16 DNA hybridization was 50% (12/24) in esophagus carcinoma tissues, and 37% (9/24) in para-carcinoma tissues. The results of Southern hybridization showed obviously the positive hybridizing band in the zone of 7.2 kb in smear. It implied that HPV 16 DNA was possibly integrated into the tissue's DNA of esophagus carcinoma. In the fetal esophagus tissues as negative control, 3 cases out of 4 showed positive results. The significance has to be further investigated. This experiment has studied the status of HPV 16 type in the tissues of esophagus carcinoma and para-carcinoma at molecular level. This is just a study of the virus etiology of esophagus carcinoma.
Li
Y
Y
Institute of Cancer Research.
Huang
G
G
Xiao
H
H
Huang
Y
Y
Mao
T
T
Deng
W
W
D015139:Blotting, Southern; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D004938:Esophageal Neoplasms / Q000382:microbiology*; D006801:Humans; D015151:Immunoblotting; D027383:Papillomaviridae / Q000302:isolation & purification*; D014412:Tumor Virus Infections
D015342:DNA Probes; D004279:DNA, Viral