Accurate evaluation of the sizes of DNA fragments (from 30 to 4700 kb) in pulse field gel electrophoresis.
Construction of long-range genomic maps by pulse field gel electrophoresis requires optimum resolution of large DNA fragments. Using the transverse-alternating field electrophoresis system, we describe a method to accurately evaluate the sizes of fragments generated by rare-cutter digestions within the 30-4700-kb range. A protocol generating large (greater than 1000 kb) molecules by partial digestion is also reported.
Crété
N
N
Hôpital Necker Enfants Malades 149, Paris, France.
Delabar
J M
JM
Sinet
P M
PM
Créau-Goldberg
N
N
D010582:Bacteriophage lambda / Q000235:genetics; D002478:Cells, Cultured; D015825:Chromosomes, Fungal; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005819:Genetic Markers; D006801:Humans; D010316:Particle Size; D012441:Saccharomyces cerevisiae / Q000235:genetics
D005819:Genetic Markers; D004247:DNA; D004262:DNA Restriction Enzymes