Presumptive identification of Salmonella enterica using two rapid tests.
Two rapid test for early detection of Salmonella enterica, the cytochrome oxidase test and a fluorescence test, were evaluated in 1,200 colonies which had been isolated from human feces and cultured on MacConkey and Salmonella-Shigella media. Using the fluorescence test there were no false negative results (sensitivity 100%) and of 205 positive cases 62 did not correspond biochemically to salmonella (specificity 94.1%); 44 of these 62 were positive in the cytochrome oxidase test, raising the specificity to 98.2%. In conclusion, the combination of the two tests could be very useful due to the ease of performance, low cost and excellent results obtained.
Ruiz
J
J
Seccion de Microbiología, Hospital Virgen de la Arrixaca, Murcia, Spain.
Varela
M C
MC
Sempere
M A
MA
Lopez
M L
ML
Gomez
J
J
Oliva
J
J
D003470:Culture Media; D003576:Electron Transport Complex IV; D005243:Feces / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D011237:Predictive Value of Tests; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D003470:Culture Media; D003576:Electron Transport Complex IV