Role of cAMP in regulation of activity of acid hydrolases of rat heart and liver during ischemia and after recirculation.
The interrelation between intracellular cAMP content and activity of lysosomal hydrolases was studied in rat liver and heart during ischemia of varying genesis and after recirculation. The activity of acid phosphatase (AP) and cathepsin D (CD) was determined in the fraction enriched with lysosomes (FEL) and in the supernatant fraction (SF) at 30,000 x g. Ischemia of isolated perfused heart of 20 to 60 min as described by Langendorff was accompanied by an increase in the SF/FEL ratio. Postischemic reperfusion resulted in a further increase in this ratio. In a terminal state induced by cardiac arrest of 10 min and within the first postresuscitation hours the SF/FEL ratio in the rat liver also increased. Processing of the liver FEL with 0.025% concentration of detergent Triton X-100 was also indicative of lability of lysosomal membranes during recirculation. The intracellular cAMP content changed differently. During ischemia of the myocardium, the cAMP level rose by 40 min and remained increased after 20 and 40 min of reperfusion. The cAMP content in the liver decreased after 10 min of circulatory arrest and increased in the postresuscitation period achieving its peak 4 h after resuscitation. Intra-abdominal injection of lyposomes with incapsulated cAMP to rats in the postresuscitation period and the study of the effect of dibutyryl-cAMP, caffeine and isoproterenol on the activity of acid hydrolases of ischemic heart and after postischemic reperfusion showed that an increase in the cAMP content achieved in various ways was conducive to stabilization of lysosomal membranes.
Molchanova
L V
LV
Institute for General Reanimatology, USSR Academy of Medical Sciences, Moscow.
Nickulina
S E
SE
Ivanova
T N
TN
Ivanov
A I
AI
Polyskova
E D
ED
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D002402:Cathepsin D / Q000378:metabolism*; D000242:Cyclic AMP / Q000502:physiology*; D008099:Liver / Q000098:blood supply / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000201:enzymology*
D000242:Cyclic AMP; D000135:Acid Phosphatase; D002402:Cathepsin D