title: Comparison of four non-radioactive and 35S-based methods for the detection of human papillomavirus DNA by in situ hybridization.
abstract: Human papillomavirus DNA was detected in 40 condylomatous lesions of various sites (vulva, cervix, larynx, penis and anus) by in situ hybridization using 35S-labelled probes and four non-radioactive probes to compare the various sensitivities of these techniques on the same material (formalin-fixed and paraffin-embedded sections). Radioactive probes yielded 28 positive results out of 40 (70%). Sulphonated probes (HybriCyte kit) also gave 28 positive results with a fine pattern of hybridization grains and equal sensitivity to 35S-labelled probes. Biotinylated and digoxigenin-labelled probes gave analogous results (25 positive reactions with the PathoGene kit, 26 with the Viratype kit, and 25 with digoxigenin-labelled probes) but are slightly less sensitive than radiolabelled and sulphonated probes especially when the signal is weak.
authors.affiliation: Laboratoire Pol Bouin, INSERM U 314, Hôpital Maison Blanche, Reims, France.
authors.forename: C
authors.initials: C
authors.lastname: Clavel
authors.forename: I
authors.initials: I
authors.lastname: Binninger
authors.forename: M C
authors.initials: MC
authors.lastname: Boutterin
authors.forename: M
authors.initials: M
authors.lastname: Polette
authors.forename: P
authors.initials: P
authors.lastname: Birembaut
mesh_terms: D000293:Adolescent; D000368:Aged; D000369:Aged, 80 and over; D001710:Biotin; D002648:Child; D002675:Child, Preschool; D003218:Condylomata Acuminata / Q000382:microbiology; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D004076:Digoxigenin; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008722:Methods; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D013462:Sulfur Radioisotopes
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D013462:Sulfur Radioisotopes; D001710:Biotin; D004076:Digoxigenin