title: Human herpesvirus-6 infection may predispose cells to superinfection by other viruses.
abstract: Double infection of cells by HHV-6, Epstein-Barr virus (EBV) and by human immunodeficiency virus (HIV-1) can enhance viral effects though genetic transactivation. It remained to be clarified, however, by which mechanism different viruses may enter the same cell. We have shown that HHV-6 infection of immature lymphoid cells rigidifies the cytoplasmic membrane and causes receptor proteins for viruses such as CD4 for HIV-1 and CR2 for EBV to be expressed. In our experiments, HHV-6 infected cells were superinfected by HIV1 and caused enhanced cell death. The mechanisms by which receptors were expressed after HHV-6 infection appears independent of cell membrane rigidification alone and is suppressed by cycloheximide only to a certain extent.
authors.affiliation: Immunopathology Laboratory, University of Cologne, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schonnebeck
authors.forename: G R
authors.initials: GR
authors.lastname: Krueger
authors.forename: M
authors.initials: M
authors.lastname: Braun
authors.forename: M
authors.initials: M
authors.lastname: Fischer
authors.forename: B
authors.initials: B
authors.lastname: Koch
authors.forename: D V
authors.initials: DV
authors.lastname: Ablashi
authors.forename: N
authors.initials: N
authors.lastname: Balachandran
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D002472:Cell Transformation, Viral; D002788:Cholesterol Esters / Q000494:pharmacology; D015342:DNA Probes; D004279:DNA, Viral / Q000302:isolation & purification; D015497:HIV-1 / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D015654:Herpesvirus 6, Human / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D008560:Membrane Fluidity / Q000187:drug effects; D009693:Nucleic Acid Hybridization
chemical_list: D002788:Cholesterol Esters; D015342:DNA Probes; D004279:DNA, Viral; C013440:cholesteryl succinate