Comparison of four non-radioactive and 35S-based methods for the detection of human papillomavirus DNA by in situ hybridization.
Human papillomavirus DNA was detected in 40 condylomatous lesions of various sites (vulva, cervix, larynx, penis and anus) by in situ hybridization using 35S-labelled probes and four non-radioactive probes to compare the various sensitivities of these techniques on the same material (formalin-fixed and paraffin-embedded sections). Radioactive probes yielded 28 positive results out of 40 (70%). Sulphonated probes (HybriCyte kit) also gave 28 positive results with a fine pattern of hybridization grains and equal sensitivity to 35S-labelled probes. Biotinylated and digoxigenin-labelled probes gave analogous results (25 positive reactions with the PathoGene kit, 26 with the Viratype kit, and 25 with digoxigenin-labelled probes) but are slightly less sensitive than radiolabelled and sulphonated probes especially when the signal is weak.
Clavel
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C
Laboratoire Pol Bouin, INSERM U 314, Hôpital Maison Blanche, Reims, France.
Binninger
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I
Boutterin
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MC
Polette
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M
Birembaut
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P
D000293:Adolescent; D000368:Aged; D000369:Aged, 80 and over; D001710:Biotin; D002648:Child; D002675:Child, Preschool; D003218:Condylomata Acuminata / Q000382:microbiology; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D004076:Digoxigenin; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008722:Methods; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D013462:Sulfur Radioisotopes
D015342:DNA Probes; D004279:DNA, Viral; D013462:Sulfur Radioisotopes; D001710:Biotin; D004076:Digoxigenin