Mapping of a new RFLP marker RN1 (DXS369) close to the fragile site FRAXA on Xq27-q28.
A new polymorphic DNA marker RN1, defining locus DXS369, was recently isolated. Using different somatic cell hybrids, RN1 was mapped between markers 4D-8 and U6.2. We have narrowed the localization of RN1 to the region between 4D-8 and FRAXA by genetic mapping in fragile X [fra(X)] families. Combined with information from other reports, the following order of loci on Xq27-q28 is suggested: cen-F9-(DXS105-DXS152)-DXS98-DXS369-FRAXA- DXS304-(DXS52-DXS15-F8)-tel. The locus DXS369 is closely linked to FRAXA, with a peak lodscore of 18.5 at a recombination fraction of 0.05. Therefore, RN1 is a useful probe for carrier detection and prenatal diagnosis in fra(X) families.
Oostra
B A
BA
Department of Cell Biology, Erasmus University, Rotterdam, The Netherlands.
Majoor-Krakauer
D F
DF
van Hemel
J O
JO
Bakker
E
E
Callen
D F
DF
Schmidt
M
M
van Oost
B A
BA
D000818:Animals; D002874:Chromosome Mapping; D015342:DNA Probes / Q000235:genetics*; D005600:Fragile X Syndrome / Q000175:diagnosis / Q000235:genetics*; D006580:Genetic Carrier Screening; D006801:Humans; D006822:Hybrid Cells; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D011296:Prenatal Diagnosis; D014960:X Chromosome
D015342:DNA Probes