title: Isolation of Gln- mutants through transposon, Tn917, mutagenesis in Bacillus brevis.
abstract: Transposon, Tn917, carried on pTV1 plasmid has been used successfully to mutagenise Bacillus brevis. The transposon showed preference for insertion at an "aro" site. A second insertional event after elimination of the preferred site with ethidium bromide/acridine orange treatment has permitted isolation of Gln- mutants in B. brevis.
authors.affiliation: Department of Biochemistry, G.B. Pant University of Agriculture & Technology, Pantnagar.
authors.forename: H S
authors.initials: HS
authors.lastname: Mishra
authors.forename: D P
authors.initials: DP
authors.lastname: Mishra
authors.forename: G K
authors.initials: GK
authors.lastname: Garg
mesh_terms: D001407:Bacillus / Q000235:genetics*; D004251:DNA Transposable Elements; D005973:Glutamine / Q000235:genetics; D016254:Mutagenesis, Insertional
chemical_list: D004251:DNA Transposable Elements; D005973:Glutamine