Cyclic AMP-receptor proteins in heart muscle of rats flown on Cosmos 1887.
A frequent cellular response to organismal stress is the increase in ligand binding by beta-adrenergic receptors. The extracellular signal is amplified by intracellular increases in cyclic AMP and the ensuing activation of cyclic AMP-dependent protein kinase (cAPK). The molecular mechanisms involve the binding of cyclic AMP to regulatory (R) subunits of cAPK, thus freeing the catalytic subunit for protein phosphorylation. This study was carried out to determine the cellular compartmentalization of the cyclic AMP-receptor proteins in heart ventricular tissue obtained from rats flown on the Cosmos 1887 mission. Photoaffinity labeling of soluble and particulate cell fractions with an [32P]-8-azido analog of cyclic AMP was followed by electrophoretic separation of the proteins and by autoradiographic identification of the labeled isoforms of cAPK R subunits. The results showed that RII in the particulate subcellular fraction was significantly decreased in heart cells from rats in the flight group when compared to controls. Protein banding patterns in both the cytoplasmic fraction and in a fraction enriched in chromatin-bound proteins showed some variability in tissues of individual animals, but exhibited no changes that could be directly attributed to flight conditions. No significant change was apparent in the distribution of RI or RII cyclic AMP binding in the soluble fractions. These findings indicate that the cardiac cell integrity or its protein content is not compromised under flight conditions. There is, however, what appears to be an adaptive molecular response which can be detected using microanalytical methods, indicating that a major hormone regulated mechanism may be affected during some phase of travel in space.
Mednieks
M I
MI
Department of Pediatrics, University of Chicago, Pritzker School of Medicine, IL 60637.
Popova
I A
IA
Grindeland
R E
RE
D000818:Animals; D002395:Catecholamines / Q000502:physiology; D002373:Cyclic AMP Receptor Protein / Q000096:biosynthesis / Q000737:chemistry*; D009206:Myocardium / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D013026:Space Flight
D002395:Catecholamines; D002373:Cyclic AMP Receptor Protein; D011943:Receptors, Adrenergic, beta; D011494:Protein Kinases