Phenotypic characterization of white cells in white cell-reduced red cell concentrate using flow cytometry.
The residual white cell (WBC) content of donated units of red cell concentrate rendered WBC-reduced by filtration through commercially available polyester filters was quantified and phenotypically analyzed. All studies were performed by flow cytometery. Quantification studies were performed with a DNA/RNA fluorophore, propidium iodide. WBC subset analyses were performed with fluorescence-labeled monoclonal antibodies directed against various cluster differentiation (CD) loci. The results indicate that the filter removes in excess of 3 log10 total WBCs from the red cell components and depletes granulocytes to or beyond the specific assay's sensitivity of 3 log10. Total T and B cells, T4 and T8 lymphocytes, and monocytes are reduced by approximately 4 log10. These analyses provide plausible explanations for the clinical success of the filter and suggest other potential applications.
Wenz
B
B
Department of Laboratory Medicine, Albert Einstein College of Medicine, Bronx, New York.
Burns
E R
ER
D001781:Blood Component Removal / Q000379:methods*; D003587:Cytomegalovirus / Q000302:isolation & purification; D004912:Erythrocytes; D005374:Filtration; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000209:etiology; D006801:Humans; D007958:Leukocyte Count; D010641:Phenotype