[Effect of unsaponifiable extracts of avocado and soybean (Piasclédine) on the collagenolytic action of cultures of human rheumatoid synoviocytes and rabbit articular chondrocytes treated with interleukin-1].
In this work, the authors have studied the effect of advocate/soya-bean extracts (Piasclédine) on the collagenolytic activity of cultured rabbit articular chondrocytes and human rheumatoid synovial cells. Incubation of these cells for 48 h with 10 micrograms/ml of Piasclédine show that this drug slightly increases collagenase production. As expected, incubation of these cells with interleukin-1 (100 pg/ml) induces an important release of collagenase. Piasclédine partially reverses the effect of interleukin-1 on synovial cells and totally abolishes its action on chondrocytes. Moreover, incubation of the two cell types for 5 days with Piasclédine prior to a 48 h-exposure to interleukin-1 prevents partially the effect of interleukin-1. These data suggest a potential role for Piasclédine to limit the deleterious effects of interleukin-1 in osteoarticular diseases by reducing the capacity of this cytokine to stimulate collagenase production by synoviocytes and chondrocytes.
Mauviel
A
A
Laboratoire de Biochimie du Tissu Conjonctif, CHU, Caen.
Loyau
G
G
Pujol
J P
JP
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D004338:Drug Combinations; D004341:Drug Evaluation; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D003012:Microbial Collagenase; D010447:Peptide Hydrolases / Q000378:metabolism*; D010840:Phytosterols / Q000494:pharmacology* / Q000627:therapeutic use; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011817:Rabbits; D012216:Rheumatic Diseases / Q000188:drug therapy*; D013583:Synovial Membrane / Q000187:drug effects / Q000201:enzymology*; D013997:Time Factors; D014810:Vitamin E / Q000494:pharmacology* / Q000627:therapeutic use
D004338:Drug Combinations; D007375:Interleukin-1; D010840:Phytosterols; D010936:Plant Extracts; C003884:piascledine; D014810:Vitamin E; D003094:Collagen; D010447:Peptide Hydrolases; D003012:Microbial Collagenase
Effet des insaponifiables d'avocat/soja (Piasclédine) sur l'activité collagénolytique de cultures de synoviocytes rhumatoïdes humains et de chondrocytes articulaires de lapin traités par l'interleukine-1.