Chromosomal insertion of TOL transposons in Pseudomonas aeruginosa PAO.
Insertions of the TOL plasmid transposons Tn4651 and Tn4653 into the Pseudomonas aeruginosa PAO chromosome were isolated by a temperature selection technique. The locations and orientations of 16 insertions were determined by pulsed field gel electrophoresis and Southern hybridization with genomic and TOL DNA probes. All insertions occurred within a 334 kb region of the chromosome (representing less than 6% of the genome) with nine of the inserts clustered within a 10 kb area. Each transposon was able to insert in either orientation. An internal duplication of the 39 kb excisable region of pWW0 was seen in two independent insertions.
Sinclair
M I
MI
Department of Genetics and Developmental Biology, Monash University, Clayton, Victoria, Australia.
Holloway
B W
BW
D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D004251:DNA Transposable Elements; D004269:DNA, Bacterial