Absorption spectra of viral components of Sendai virus in the wavelength region from 130 to 320 nm.
Using synchrotron radiation as a light source, the absorption spectra of purified viral components of the Sendai virus, i.e. messenger RNA, lipids, spike (envelope) proteins, reconstructed envelopes, core proteins and whole virions, were obtained in the wavelength region 130-320 nm by measuring the transmission of thin films. Viral (messenger) RNA two peaks at 260 and 190 nm, and a large increase below 160 nm. The absorption spectrum of lipids exhibited a broad peak at 190 nm and a very sharp increase below 160 nm. With spike proteins, a slight peak at 280 nm and a shoulder at 230 nm were observed in addition to a sharper peak at 190 nm and a rather slow increasing absorption below 160 nm. Reconstructed envelopes showed the features of a combination of lipids and proteins. The absorption spectra of core proteins and whole virions exhibited similar characteristics to spike proteins. Conventional UV data were also obtained in the wavelength range 210-320 nm with RNA and lipids. The UV and synchrotron radiation data were in good agreement in terms of the mass absorption coefficients. The molecular splitting of spike proteins was also examined. Proteins gave more diffuse reflection than their subunits, causing a reduction in absorption. This was explained by a loss of transparency with increasing molecular weight.
Megumi
T
T
Research Institute for Science and Technology, University of Osaka Prefecture, Japan.
Nishikawa
R
R
Fujita
S
S
Saito
M
M
Ito
T
T
D000818:Animals; D002642:Chick Embryo; D008051:Lipid Bilayers; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification; D010222:Parainfluenza Virus 1, Human / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry* / Q000302:isolation & purification; D012367:RNA, Viral / Q000737:chemistry* / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet; D014764:Viral Proteins / Q000737:chemistry* / Q000302:isolation & purification; D014771:Virion / Q000737:chemistry
D008051:Lipid Bilayers; D008055:Lipids; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins