title: Comparison of the primary sequences of two potato tuber ADP-glucose pyrophosphorylase subunits.
abstract: Near-full-length cDNA clones to the small and large subunit of the heterotetrameric potato tuber ADP-glucose pyrophosphorylase have been isolated and characterized. The missing amino terminal sequence of the small subunit has also been elucidated from its corresponding genomic clone. Primary sequence comparisons revealed that each potato subunit had less identity to each other than to their homologous subunit from other plants. It also appeared that the smaller subunit is more conserved among the different plants and the larger subunit more divergent. Amino acid comparisons of both potato tuber sequences to the Escherichia coli ADP-glucose pyrophosphorylase sequence revealed conserved regions important for both catalytic and allosteric function of the bacterial enzyme.
authors.affiliation: Program in Biochemistry and Biophysics, Washington State University, Pullman 99164-6340.
authors.forename: P A
authors.initials: PA
authors.lastname: Nakata
authors.forename: T W
authors.initials: TW
authors.lastname: Greene
authors.forename: J M
authors.initials: JM
authors.lastname: Anderson
authors.forename: B J
authors.initials: BJ
authors.lastname: Smith-White
authors.forename: T W
authors.initials: TW
authors.lastname: Okita
authors.forename: J
authors.initials: J
authors.lastname: Preiss
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D051599:Glucose-1-Phosphate Adenylyltransferase; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D010944:Plants / Q000201:enzymology / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics*
chemical_list: D046911:Macromolecular Substances; D004247:DNA; D009713:Nucleotidyltransferases; D051599:Glucose-1-Phosphate Adenylyltransferase