Widespread expression of MyoD genes in Xenopus embryos is amplified in presumptive muscle as a delayed response to mesoderm induction.
The MyoD gene codes for an important regulatory factor in skeletal myogenesis. To explore the relationship between mesoderm induction in Xenopus embryos and expression of MyoD, I have monitored MyoD mRNA levels in normal embryos and cultured explants by RNase protection. Transcription from the two Xenopus MyoD gene copies is activated weakly across the whole embryo at the midblastula transition, and this activation occurs in the absence of mesoderm induction. In response to induction this basal expression is amplified 50- to 100-fold, but in animal-pole explants 6-10 hr elapse before induced mRNAs appear, and this induction requires prior protein synthesis. The promiscuous transcripts disappear from animal explants at a time when induction "competence" is lost, suggesting a link between these events. The data highlight a broad, but transient, permissiveness for MyoD expression in embryos, which is propagated and amplified only in presumptive muscle in response to induction. Moreover, muscle-specific MyoD expression is a relatively late (postgastrulation) event in the mesoderm-induction cascade.
Harvey
R P
RP
Molecular Biology Unit, Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
D000199:Actins / Q000032:analysis / Q000235:genetics; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005775:Gastrula / Q000502:physiology; D015870:Gene Expression; D005786:Gene Expression Regulation; D007700:Kinetics; D008648:Mesoderm / Q000502:physiology*; D009124:Muscle Proteins / Q000032:analysis / Q000235:genetics*; D009132:Muscles / Q000196:embryology* / Q000502:physiology; D017570:MyoD Protein; D009866:Oogenesis; D009924:Organ Culture Techniques; D014158:Transcription, Genetic / Q000187:drug effects; D014982:Xenopus laevis / Q000196:embryology*
D000199:Actins; D004268:DNA-Binding Proteins; D009124:Muscle Proteins; D017570:MyoD Protein; D016222:Fibroblast Growth Factor 2; D003513:Cycloheximide