The potential of perturbed angular correlation of gamma rays as a tool for dynamic studies of peptides/proteins.
Four peptides and serum albumin have been derivatized with the bicyclic anydride of diethylenetriamine-pentaacetic acid. Procedures were developed to isolate the labelled species and determine the degree of derivatization. By using perturbed angular correlation of gamma-ray spectroscopy it is possible, through the determination of rotational correlation times, to decide whether labelled peptides interact with other molecules (receptors). In the case of the peptide cholecystokinin it is shown that the interaction between the peptide and its corresponding polyclonal antibody can be determined down to 1 pmol hormone. Experiments on 111In- and 111mCd-labelled Gly-Trp showed that, where the 111Cd PAC spectrum directly reflected the rotational motion of the molecule, the 111In PAC spectrum was affected by the nuclear transitions to 111mCd.
Bauer
R
R
Department of Mathematics and Physics, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Atke
A
A
Danielsen
E
E
Marcussen
J
J
Olsen
C E
CE
Rehfeld
J
J
Saermark
T
T
Schneider
D
D
Vilhardt
H
H
Zeppezauer
M
M
D016880:Anisotropy; D002104:Cadmium / Q000737:chemistry; D005720:Gamma Rays; D007205:Indium Radioisotopes / Q000737:chemistry*; D007553:Isotope Labeling; D007700:Kinetics; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D004369:Pentetic Acid; D010455:Peptides / Q000737:chemistry*; D013051:Spectrometry, Gamma
D007205:Indium Radioisotopes; D046911:Macromolecular Substances; D010455:Peptides; D002104:Cadmium; D004369:Pentetic Acid