Detection and typing of human papillomaviruses in cervical smears by an original application of the polymerase chain reaction.
The detection and typing of human papillomaviruses on cervical smears were performed by means of a new application of the polymerase chain reaction allowing easier and faster detection of the amplification product. This application consisted of a combination of two series of amplifications and the use of primers labelled with biotin and with 125 iodine on a reporter group for the second amplification. The final amplification product was detected by counting the radioactivity after incubation of the media in avidin-coated tubes. This test was compared with conventional methods of detection by electrophoresis and Southern blot and its specificity was confirmed. The study of a series of 52 patients demonstrated a higher prevalence of type 16 in relation to type 6/11 and 18 and a correlation between the degree of dysplasia and the frequency of oncogenic types 16 and 18. This new application could facilitate studies of the prevalence of HPV in large series of cervical smears.
Charlotte
F
F
Laboratoire de biologie cellulaire et moléculaire, hôpital Saint Antoine, Paris, France.
Olivier
J L
JL
Chypre
C
C
Beuret
T
T
Sadoul
G
G
Chatelet
F
F
Luboinski
J
J
Marchand
J
J
Bereziat
G
G
D001360:Avidin; D001483:Base Sequence; D001710:Biotin; D015139:Blotting, Southern; D002584:Cervix Uteri / Q000382:microbiology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007455:Iodine; D007553:Isotope Labeling; D008863:Microspheres; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*; D014626:Vaginal Smears
D015342:DNA Probes; D004279:DNA, Viral; D001360:Avidin; D001710:Biotin; D007455:Iodine