title: Secretion of myeloperoxidase isoforms by human neutrophils.
abstract: The human neutrophil lysosomal enzyme, myeloperoxidase (MPO), exists in three major and chromatographically distinct forms, MPO I, MPO II, and MPO III. We used cation-exchange medium-pressure liquid chromatography and kinetic microenzyme assay (or spectrophotometric monitoring) to analyze the secretion of MPO isoforms by neutrophils exposed to N-formylmethionylleucylphenylalanine (FMLP), digitonin, the ionophore A23187, and serum-opsonized zymosan A (SOZ). All three MPO isomers were released into the fluid phase after neutrophils were exposed to these secretagogues. A significant proportional increase in MPO I was released when neutrophils were stimulated with SOZ. MPO I was released in higher proportions than found in the whole cell constituency when neutrophils were stimulated with FMLP + cytochalasin B, A23187, and digitonin, but this was not statistically significant.
authors.affiliation: Section of Oral Biology, UCLA School of Dentistry, Center for the Health Sciences 90024.
authors.forename: K T
authors.initials: KT
authors.lastname: Miyasaki
authors.forename: J P
authors.initials: JP
authors.lastname: Song
authors.forename: R K
authors.initials: RK
authors.lastname: Murthy
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D004072:Digitonin / Q000494:pharmacology; D006801:Humans; D007536:Isomerism; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism*; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D009195:Peroxidase; D004072:Digitonin