Morphological effects of the catecholestrogens on cells of the seminiferous tubules of Sprague-Dawley rats.
Estradiol-17 beta (E2) and the two catecholestrogens 2-OHE2 and 4-OHE2, when daily administered at low doses of 10-40 ng/rat, were cytotoxic to the seminiferous epithelium. The structural changes seen after seven days exposure included abnormal meiotic type II cells with uneven chromosome distribution, the formation of binucleated and multinucleated giant cells, of which many were sloughed into the lumina of the seminiferous tubules. The effect of the 4-OHE2 metabolites were always more pronounced that that of 2-OHE2 or E2. After 21 daily exposures, 4-OHE2 proved to be very toxic, the seminiferous tubules were markedly denuded and numerous giant cells were present in the lumina. The catecholestrogens also caused a significant lowering (P less than 0.02) of testosterone serum levels after eight days exposure. E2 at 40 ng/rat/day had no effect on testosterone production. At these low doses the catecholestrogens did not affect gonadotropin release after eight days exposure. Our results indicate that the morphological lesions could not exclusively be attributed to testosterone withdrawal and that a direct effect on developing spermatids is also indicated.
Seegers
J C
JC
Dept. of Physiology, Medical Faculty, University of Pretoria, South Africa.
van Aswegen
C H
CH
Nieuwoudt
B L
BL
Joubert
W S
WS
D000818:Animals; D004958:Estradiol / Q000031:analogs & derivatives* / Q000633:toxicity; D002393:Estrogens, Catechol / Q000633:toxicity*; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012671:Seminiferous Tubules / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013091:Spermatogenesis / Q000187:drug effects; D013739:Testosterone / Q000378:metabolism
D002393:Estrogens, Catechol; D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; C001390:2-hydroxyestradiol; C014036:4-hydroxyestradiol