title: Influence of Ca2+ depletion on cytoskeleton and nucleolus morphology in Trypanosoma brucei.
abstract: Trypanosoma brucei bloodstream forms were incubated in a calcium-free medium containing 10 microM ethyleneglycol-bis-(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA). Under these conditions, addition of 5 microM calcium ionophore A23187 led to striking morphological alterations, as judged by light and electron microscopy. The cytoskeleton of trypanosomes consists of a subpellicular corset of microtubules. Characteristically four of these microtubules are attached invariantly to an extension of the endoplasmic reticulum at the flagellar attachment site. Specifically in this area calcium depletion led to the polymerization of additional microtubules and to a retraction of the endoplasmic reticulum extension from its usual position. Additionally, A23187 led to nucleolus segregation, as revealed by immunocytochemistry using antibodies against DNA and fibrillarin, respectively. Nucleolus segregation, but not microtubule accumulation, was also obtained by using 20 microM camptothecin, a specific inhibitor of topoisomerase I. Our data suggest that intracellular calcium regulation might be important for specific depolymerization/polymerization reactions during the course of cell division and the formation of functional ribosomes.
authors.affiliation: Physiologisch-chemisches Institut Universität, Tübingen/Bundesrepublik, Deutschland.
authors.forename: P M
authors.initials: PM
authors.lastname: Selzer
authors.forename: P
authors.initials: P
authors.lastname: Webster
authors.forename: M
authors.initials: M
authors.lastname: Duszenko
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002166:Camptothecin / Q000494:pharmacology; D002466:Cell Nucleolus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology / Q000302:isolation & purification; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004247:DNA / Q000276:immunology / Q000302:isolation & purification; D004533:Egtazic Acid / Q000494:pharmacology; D005629:Frozen Sections; D008854:Microscopy, Electron; D011506:Proteins / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D059004:Topoisomerase I Inhibitors; D014346:Trypanosoma brucei brucei / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D011506:Proteins; D011773:Pyruvates; D059004:Topoisomerase I Inhibitors; C047458:fibrillarin; D000001:Calcimycin; D004533:Egtazic Acid; D004247:DNA; D002118:Calcium; D002166:Camptothecin