Interactions of organic hydroperoxides with heme proteins.
The decomposition of p-methylbenzyl hydroperoxide by cytochrome c and other selected heme systems in the absence of reducing agents was investigated. p-Methylbenzaldehyde was identified as the major product. A mechanism for this reaction has been suggested. H2O2 and tertiary cumyl hydroperoxide do not react under these conditions. The ability of organic hydroperoxides to act as oxygen donors in the cytochrome-mediated 7-ethoxyresorufin-O-deethylation was studied. Cumyl and tert.butyl hydroperoxides are able to substitute oxygen in the absence of NADPH while p-methylbenzyl hydroperoxide is not.
Zídek
L
L
Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czechoslovakia.
Machala
M
M
Skurský
L
L
D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors; D000818:Animals; D001555:Benzene Derivatives / Q000737:chemistry; D001593:Benzyl Compounds / Q000737:chemistry; D002642:Chick Embryo; D002645:Chickens; D002855:Chromatography, Thin Layer; D003574:Cytochrome c Group / Q000378:metabolism; D004531:Eggs / Q000032:analysis; D006420:Hemeproteins / Q000737:chemistry*; D008706:Methemoglobin / Q000737:chemistry; D009249:NADP / Q000378:metabolism; D010545:Peroxides / Q000737:chemistry*; D011485:Protein Binding; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D001555:Benzene Derivatives; D001593:Benzyl Compounds; D003574:Cytochrome c Group; D006420:Hemeproteins; D010545:Peroxides; D009249:NADP; C075152:4-methylbenzyl hydroperoxide; D008706:Methemoglobin; D000426:Alcohol Dehydrogenase; C007164:cumene hydroperoxide