Derivation of lifespan extended cell line from simian virus 40 infected human uterine endocervical epithelial cells cultured on collagen gel.
Primary culture of human uterine endocervical epithelial cells was performed. When cells were cultured on a plastic dish, these cells developed into a flat form, then lost their proliferative ability in a short time during continuous subculture. On the other hand, when cells were cultured on collagen gel and subcultured by explanation of this cell containing gel, the cells took on a roughly ovoid rather than a flat shape and active proliferation was attained which maintained confluency for as long as 8 weeks. Furthermore by adopting the Simian Virus 40 in the culture system for these cells, it was possible to extend the lifespan to approximately twice that of the control cell group.
Kanoh
H
H
Department of Gynecology, Osaka University.
D002470:Cell Survival; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000166:cytology* / Q000382:microbiology; D003094:Collagen; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D005260:Female; D005782:Gels; D006801:Humans; D013539:Simian virus 40 / Q000254:growth & development*
D005782:Gels; D003094:Collagen