Superoxide anion production by lipoamide dehydrogenase redox-cycling: effect of enzyme modifiers.
Redox-cycling of porcine heart lipoamide dehydrogenase in the presence of NADH and oxygen produced O2-. (NADH-oxidase activity) as demonstrated by (a) reduction of cytochrome c; (b) reduction of the Fe(III)-ADP complex; (c) lucigenin luminescence and (d) the inhibitory effect of superoxide dismutase. NAD+ and p-chloromercuribenzoate inhibited O2-. generation whereas arsenite enhanced it. Comparison of heart and yeast enzyme preparations revealed a close correlation between lipoamide reductase and NADH-oxidase activities. It is concluded that O2-. production is a molecular property of lipoamide dehydrogenase.
Grinblat
L
L
Centro de Investigaciones Bioenergéticas, Facultad de Medicina, Buenos Aires, Argentina.
Sreider
C M
CM
Stoppani
A O
AO
D000166:Acridines / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003454:Cryptococcus / Q000201:enzymology; D003574:Cytochrome c Group / Q000378:metabolism; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology; D009243:NAD / Q000494:pharmacology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D013552:Swine; D020245:p-Chloromercuribenzoic Acid
D000166:Acridines; D018053:Arsenites; D002729:Chloromercuribenzoates; D003574:Cytochrome c Group; D005290:Ferric Compounds; D009243:NAD; D013481:Superoxides; C033472:10,10'-dimethyl-9,9'-biacridinium; D020245:p-Chloromercuribenzoic Acid; D000244:Adenosine Diphosphate; D013482:Superoxide Dismutase; D009247:NADH, NADPH Oxidoreductases; D008058:Dihydrolipoamide Dehydrogenase; C015001:arsenite; D001151:Arsenic; D010100:Oxygen