Neurotensin effects on calcium/calmodulin-dependent protein phosphorylation in rat neostriatal slices.
Neurotensin (NT) is an endogenous brain tridecapeptide for which high affinity binding sites exist in the central nervous system. We have investigated the effects of NT incubation with rat neostriatal slices on calcium/calmodulin (Ca/CaM)-dependent protein phosphorylation. Slices were incubated with NT (5 or 50 nM) for 3, 10, 16 or 30 min followed by in vitro phosphorylation, electrophoresis and autoradiography. NT significantly altered the phosphorylation of a 62 kDa protein which is likely the beta subunit of the Ca/CaM dependent protein kinase. These changes may reflect the ability of NT to influence calcium mediated signal transduction.
Kasckow
J
J
Department of Psychiatry, Duke University Medical Center, Durham, NC 27710.
Cain
S T
ST
Nemeroff
C B
CB
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002118:Calcium / Q000494:pharmacology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002147:Calmodulin / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008297:Male; D008970:Molecular Weight; D009496:Neurotensin / Q000494:pharmacology*; D010750:Phosphoproteins / Q000302:isolation & purification* / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002147:Calmodulin; D046911:Macromolecular Substances; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D009496:Neurotensin; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002118:Calcium