title: The priB gene encoding the primosomal replication n protein of Escherichia coli.
abstract: The gene encoding protein n of the Escherichia coli primosome has been discovered in the rpsF-rpsR-rplI ribosomal protein operon and designated priB. The low copy number of PriB protein and the distinctive codon usage of its gene argue against its being a ribosomal protein. A strain which overproduces PriB was constructed and has been used to purify the protein to homogeneity. The overproduced protein behaves like that purified from wild-type cells.
authors.affiliation: Department of Biochemistry, Stanford University School of Medicine, California 94305.
authors.forename: G C
authors.initials: GC
authors.lastname: Allen
authors.forename: A
authors.initials: A
authors.lastname: Kornberg
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D002850:Chromatography, Gel; D003062:Codon; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003062:Codon; D012269:Ribosomal Proteins