title: Targeting of the master receptor MOM19 to mitochondria.
abstract: The targeting of proteins to mitochondria involves the recognition of the precursor proteins by receptors on the mitochondrial surface followed by insertion of the precursors into the outer membrane at the general insertion site GIP. Most mitochondrial proteins analyzed so far use a mitochondrial outer membrane protein of 19 kilodaltons (MOM19) as an import receptor. The gene encoding MOM19 has now been isolated. The deduced amino acid sequence predicts that MOM19 is anchored in the outer membrane by an NH2-terminal hydrophobic sequence, while the rest of the protein forms a hydrophilic domain exposed to the cytosol. MOM19 was targeted to the mitochondria via a pathway that is independent of protease-accessible surface receptors and controlled by direct assembly of the MOM19 precursor with GIP.
authors.affiliation: Institut für Physiologische Chemie, Universität München, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schneider
authors.forename: T
authors.initials: T
authors.lastname: Söllner
authors.forename: K
authors.initials: K
authors.lastname: Dietmeier
authors.forename: C
authors.initials: C
authors.lastname: Eckerskorn
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: B
authors.initials: B
authors.lastname: Trülzsch
authors.forename: W
authors.initials: W
authors.lastname: Neupert
authors.forename: N
authors.initials: N
authors.lastname: Pfanner
mesh_terms: D000595:Amino Acid Sequence; D002451:Cell Compartmentation; D004247:DNA / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D005800:Genes, Fungal; D007425:Intracellular Membranes / Q000378:metabolism; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins
chemical_list: D005656:Fungal Proteins; C066557:MOM19 protein, Neurospora crassa; D046911:Macromolecular Substances; D008565:Membrane Proteins; D011498:Protein Precursors; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins; D004247:DNA