Endogenous biotinylated proteins in Dictyostelium discoideum.
Biotin-dependent enzymes are involved in carboxylation, decarboxylation and transcarboxylation reactions. Here, we have used sodium dodecyl sulfate polyacrylamide gel electrophoresis and electroblotting followed by probing with avidin to identify biotin-containing polypeptides in Dictyostelium discoideum. Twenty biotinyl polypeptides were visualized, with a 23 kDa protein appearing transiently. Based upon the molecular mobility of the biotinyl polypeptides, D. discoideum may contain the biotin-dependent enzymes acetyl CoA carboxylase, proprionyl CoA carboxylase, pyruvate carboxylase, and 3-methylcrotonyl CoA carboxylase.
Lydan
M A
MA
University of Toronto, Department of Zoology, Erindale College, Mississauga, Ontario, Canada.
O'Day
D H
DH
D000103:Acetyl-CoA Carboxylase / Q000302:isolation & purification*; D000818:Animals; D001710:Biotin / Q000032:analysis*; D019735:Carbon-Carbon Ligases; D002262:Carboxy-Lyases / Q000302:isolation & purification*; D004023:Dictyostelium / Q000201:enzymology* / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000302:isolation & purification*; D006801:Humans; D008025:Ligases / Q000302:isolation & purification*; D043586:Methylmalonyl-CoA Decarboxylase; D008970:Molecular Weight; D011766:Pyruvate Carboxylase / Q000302:isolation & purification*
D005656:Fungal Proteins; D001710:Biotin; D002262:Carboxy-Lyases; D008025:Ligases; D019735:Carbon-Carbon Ligases; D011766:Pyruvate Carboxylase; D000103:Acetyl-CoA Carboxylase; C021573:methylcrotonoyl-CoA carboxylase; D043586:Methylmalonyl-CoA Decarboxylase