Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting.
Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.
Kelling
C L
CL
Department of Veterinary Science, University of Nebraska, Lincoln 68583-0905.
Kennedy
J E
JE
Rump
K K
KK
Stine
L C
LC
Paul
P S
PS
Partridge
J E
JE
D000818:Animals; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005095:Exoribonucleases / Q000378:metabolism; D009710:Nucleotide Mapping; D009841:Oligonucleotides / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis* / Q000737:chemistry; D014765:Viral Vaccines / Q000032:analysis* / Q000235:genetics
D009841:Oligonucleotides; D012367:RNA, Viral; D014765:Viral Vaccines; D005095:Exoribonucleases; C042081:exoribonuclease T