Sex steroid binding protein (SBP) receptors in estrogen sensitive tissues.
Since the discovery of a specific membrane binding site for sex steroid binding protein (SBP) in human decidual endometrium and in hyperplastic prostate numerous speculations have been raised on the existence of an additional non-receptor-mediated system for steroid hormone action. In the present work SBP cell membrane binding was investigated in human estrogen target tissues other than those previously studied either in the absence of steroids or in the presence of varying amounts (10(-10)-10(-6) M) of estradiol, testosterone and dihydrotestosterone, respectively. Plasma membranes obtained by differential centrifugation from homogenized samples of pre-menopausal endometrium, endometrium adenocarcinoma, normal liver and post-menopausal breast showed a specific binding of highly purified [125I]SBP: a major displacement of labeled SBP was elicited by radioinert SBP, while no significant displacement occurred when other human plasma proteins were used as cold competitors (molar excess ranging 500-10,000-fold). A specific, time-dependent binding of [125I]SBP was also observed in MCF-7 and in Hep-G2 cell lines. The different patterns of specific binding, observed in membranes from different tissues when SBP was liganded with different sex steroid molecules, leads us to consider the tissue individuality of the receptor as a further entity in the membrane recognition system for SBP.
Frairia
R
R
Dipartimento di Fisiopatologia Clinica, Università di Torino, Italy.
Fortunati
N
N
Berta
L
L
Fazzari
A
A
Fissore
F
F
Gaidano
G
G
D000230:Adenocarcinoma / Q000503:physiopathology; D001940:Breast / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism; D016889:Endometrial Neoplasms / Q000503:physiopathology; D004717:Endometrium / Q000502:physiology*; D004958:Estradiol / Q000378:metabolism; D004967:Estrogens / Q000502:physiology*; D005260:Female; D006801:Humans; D008099:Liver / Q000502:physiology; D008593:Menopause; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004967:Estrogens; D011956:Receptors, Cell Surface; D012738:Sex Hormone-Binding Globulin; C063572:sex hormone-binding globulin receptor; D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol