title: Purification and characterization of neurotensin receptor from rat brain with special reference to comparison between newborn and adult age rats.
abstract: Scatchard analysis of saturation curves was performed to compared newborn and adult rat neurotensin receptor using [3H] neurotensin as a tracer. The membrane fraction of newborn rat cerebral cortex has a single population of neurotensin receptor (Kd = 0.13 nM, Bmax = 710 fmol/mg protein), whereas adults have two distinct neurotensin binding sites (high affinity site, Kd1 = 0.13 nM; low affinity site, Kd2 = 20 nM). High affinity neurotensin receptor, solubilized with digitonin, was purified from newborn rat cortex by affinity chromatography. An overall purification of 14,000-fold was achieved. The binding of [3H] neurotensin to the purified receptor is saturable and specific, with a Kd of 0.45 nM. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the presence of 2-mercaptoethanol revealed purified material of a single major band of Mr = 55,000.
authors.affiliation: Department of Neuroanatomy, Osaka University Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Miyamoto-Lee
authors.forename: S
authors.initials: S
authors.lastname: Shiosaka
authors.forename: M
authors.initials: M
authors.lastname: Tohyama
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007031:Hypothalamus / Q000254:growth & development / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009496:Neurotensin / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter; D009496:Neurotensin