Genetic changes at the beta-2-adrenergic receptor locus on chromosome 5 in human colorectal carcinomas.
Matched normal/tumor DNA pairs from 44 colorectal carcinoma patients were examined for tumor-specific genetic changes using a probe for the beta-2-adrenergic receptor (ADRB2R) gene on chromosome 5. This locus (5q31-q32) maps close to the site of chromosomal deletions recently reported to occur in colorectal carcinomas and distal to the chromosomal location of the familial adenomatous polyposis (FAP) gene (5q21-q22). Our investigation shows tumor-specific allele loss or allelic rearrangement of at least 29% at the AdRb2R locus on chromosome 5 in informative cases. These results suggest that the mechanism by which colorectal carcinomas lose genetic material on chromosome 5 can affect this functional gene located distally to the FAP gene. The possible functional significance that ADRB2R gene changes may have in neoplastic progression is discussed.
Gope
R
R
Creighton Cancer Center, Creighton University School of Medicine, Omaha, Nebraska 68178.
Gope
M L
ML
Thorson
A
A
Christensen
M
M
Smyrk
T
T
Chun
M
M
Alvarez
L
L
Wildrick
D M
DM
Boman
B M
BM
D011125:Adenomatous Polyposis Coli / Q000235:genetics; D000483:Alleles; D002872:Chromosome Deletion; D002895:Chromosomes, Human, Pair 5; D015179:Colorectal Neoplasms / Q000235:genetics*; D006801:Humans; D011943:Receptors, Adrenergic, beta / Q000235:genetics*
D011943:Receptors, Adrenergic, beta