title: Lactic acid enantiomers: separation by thin-layer chromatography on silica gel plates impregnated with Cu2+.
abstract: A simple and fast method for the separation of D- and L-lactic acid enantiomers by thin-layer chromatographic silica gel precoated plates impregnated with Cu(II) is proposed. The two lactic acid enantiomers, dissolved in acetone, are deposed on these plates. D- and L-lactic acids form complexes with Cu(II), with different Rf values, suitable for radiochemical measurements. The real separation was confirmed by enzymatic tests carried out on spots of D and L forms scraped from the chromatographic plates. Total radioactivity of 14C-labeled D- and L-lactic acids was recovered. A Cu/lactic acid complex is evidenced.
authors.affiliation: Centro per lo Studio Tecnologico, Bromatologico e Microbiologico del Latte, CNR, Milano, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Cecchi
authors.forename: P
authors.initials: P
authors.lastname: Malaspina
mesh_terms: D000096:Acetone; D002855:Chromatography, Thin Layer; D003300:Copper; D005782:Gels; D007773:Lactates / Q000737:chemistry*; D019344:Lactic Acid; D012822:Silicon Dioxide; D013237:Stereoisomerism
chemical_list: D005782:Gels; D007773:Lactates; D000096:Acetone; D019344:Lactic Acid; D012822:Silicon Dioxide; D003300:Copper