Construction and expression of a recombinant adeno-associated virus that harbors a human beta-globin-encoding cDNA.
Towards a goal of using recombinant adeno-associated viruses (AAV) for the gene therapy of hemoglobinopathies we had previously constructed plasmid pAV h beta G psi 1, which contained a human beta-globin-encoding cDNA (HBB) downstream from the P40 promoter of AAV2 DNA [Ohi et al., Gene 89 (1990) 279-282]. Transfection of the plasmid into human 293 cells (embryonal kidney cell line) resulted in the expression of HBB at the mRNA level as well as rescue and replication of the recombinant AAV genome (Ohi et al., ibid.). The present study demonstrates that the replicated recombinant DNA was packaged into an intact virion by transcomplementation with pAV2 or the defective helpers, pAV delta Bam or pAVXB. The recombinant virus could be isolated by equilibrium CsCl density gradient, the density of which was about 1.4 g/cm3. The defective helpers are used to produce wild-type AAV-free recombinant AAV. The recombinant AAV were infectious and expressed chimeric mRNAs containing the HBB sequence in virus-infected 293, KB (oral epidermoid carcinoma cell line) and K562 (human erythroleukemia cell line) cells. The importance of the infectivity and expression of the recombinant AAV in hematopoietic cells is discussed in the context of gene therapy of hemoglobinopathies.
Dixit
M
M
Department of Biochemistry, School of Medicine, Meharry Medical College, Nashville, TN.
Webb
M S
MS
Smart
W C
WC
Ohi
S
S
D001773:Blood Cells / Q000382:microbiology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA; D000229:Dependovirus / Q000235:genetics* / Q000472:pathogenicity; D015316:Genetic Therapy; D005914:Globins / Q000096:biosynthesis / Q000235:genetics*; D006453:Hemoglobinopathies / Q000628:therapy; D006801:Humans; D010957:Plasmids; D014162:Transfection; D014407:Tumor Cells, Cultured
D005914:Globins; D004247:DNA