Ki-ras activation in pancreatic carcinomas of Syrian hamsters induced by N-nitrosobis(2-hydroxypropyl)amine.
Five pancreatic carcinomas induced by N-nitrosobis(2-hydroxypropyl)amine in Syrian golden hamsters were analyzed for activation of Ki-ras at codons 12 and 13, using the polymerase chain reaction and direct sequencing. The Ki-ras gene was shown to be activated in four of the five carcinomas, and the results were further confirmed by subcloning and sequencing. All the mutations involved a G-to-A transition at the second position of codon 12, which resulted in a change at the amino acid level from glycine to aspartic acid. This mutation is identical with that reported for pancreatic tumors of Syrian hamsters induced by N-nitrosobis(2-oxopropyl)amine.
Ushijima
T
T
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo.
Tsutsumi
M
M
Sakai
R
R
Ishizaka
Y
Y
Takaku
F
F
Konishi
Y
Y
Takahashi
M
M
Sugimura
T
T
Nagao
M
M
D000818:Animals; D001483:Base Sequence; D002273:Carcinogens; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000139:chemically induced / Q000235:genetics*; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D011905:Genes, ras / Q000187:drug effects*; D008647:Mesocricetus; D008969:Molecular Sequence Data; D009602:Nitrosamines; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000235:genetics*
D002273:Carcinogens; D004273:DNA, Neoplasm; D009602:Nitrosamines; C012457:diisopropanolnitrosamine