H-ras-1 polymorphism in leukemia.
We have examined leukocyte DNAs obtained from 21 patients with various types of leukemia for the Sacl polymorphism of H-ras-1. The patients included ten with B-lineage leukemia, two with T-cell leukemia, and nine with acute myelogenous leukemia. Using a genomic probe for H-ras-1 in Southern hybridizations, we found that 78% of the DNA from leukemias of myeloid origin were heterozygous. In contrast, only three of ten B-cell-derived leukemias showed heterozygosity. This difference in the number of heterozygotes vs. homozygotes in the two patient samples studied is statistically significant (P = 0.04 by Fisher's exact test). These results suggest the possibility that the H-ras-1 allelic pattern that is associated with acute myelogenous leukemia is distinct from that found in B-cell-derived malignancies.
Wadleigh
R G
RG
Section of Medical Oncology, VA Medical Center, Washington, DC 20422.
Chan
C S
CS
Cohen
M H
MH
Krasnow
S H
SH
Anderson
A
A
Detera-Wadleigh
S D
SD
D011905:Genes, ras; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D007938:Leukemia / Q000235:genetics*; D009857:Oncogenes; D012150:Polymorphism, Restriction Fragment Length
D007136:Immunoglobulins