Isolation of islets of Langerhans from the weanling pig.
A simple method for the isolation of porcine islets of Langerhans is described. The technique, based on sequential collagenase digestion and density gradient separation through Percoll at unit gravity, produces islets which secrete insulin in response to glucose stimulation in static incubation, show bi-phasic release of insulin when perifused with 16 mM glucose, and can be maintained in culture for six weeks.
Heald
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KA
Charles Hodgson Islet Laboratory, University Department of Surgery, Queen Elizabeth Hospital, Edgbaston, Birmingham, UK.
Hail
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CA
Downing
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R
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient / Q000379:methods; D046508:Culture Techniques / Q000379:methods; D007202:Indicators and Reagents; D007515:Islets of Langerhans / Q000166:cytology*; D003012:Microbial Collagenase; D011205:Povidone; D012822:Silicon Dioxide; D013552:Swine
D007202:Indicators and Reagents; C016039:Percoll; D012822:Silicon Dioxide; D003012:Microbial Collagenase; D011205:Povidone