title: Retinoic acid modulates both invasion and plasma membrane ruffling of MCF-7 human mammary carcinoma cells in vitro.
abstract: The invasiveness of MCF-7 human mammary carcinoma cells was tested in vitro via confronting cultures with embryonic chick heart fragments. Invasive (e.g. MCF-7/6) and non-invasive (e.g. MCF-7/AZ) variants were detected. Automated image analysis of time-lapse video-microscopy recordings showed that the plasma membrane ruffling activity of the invasive MCF-7/6 variant was higher than the ruffling activity of the non-invasive MCF-7/AZ variant. Addition of all-trans-retinoic acid to the culture medium (10(-6) M) inhibited both invasion and ruffling of MCF-7/6 cells, while MCF-7/AZ cells became invasive and acquired an increased ruffling by the same type of treatment. A similar opposite effect on MCF-7 cells was not found after treatment with other ligands of the nuclear steroid/thyroid receptor superfamily. Triiodo-l-thyronine (up to 10(-5) M) and beta-oestradiol (up to 10(-6) M) did not alter the invasiveness of the cells, while dexamethasone (10(-6) M) and the pure anti-oestrogen ICI 164,384 inhibited both invasion and ruffling. Our data show that retinoic acid can modulate invasiveness in opposite directions.
authors.affiliation: Department of Radiotherapy and Nuclear Medicine, University Hospital, Gent, Belgium.
authors.forename: M E
authors.initials: ME
authors.lastname: Bracke
authors.forename: N A
authors.initials: NA
authors.lastname: Van Larebeke
authors.forename: B M
authors.initials: BM
authors.lastname: Vyncke
authors.forename: M M
authors.initials: MM
authors.lastname: Mareel
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D002449:Cell Aggregation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure*; D002642:Chick Embryo; D003907:Dexamethasone / Q000494:pharmacology; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D005260:Female; D006801:Humans; D009206:Myocardium / Q000166:cytology; D009361:Neoplasm Invasiveness; D053284:Polyunsaturated Alkamides; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology; D014212:Tretinoin / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D004965:Estrogen Antagonists; D053284:Polyunsaturated Alkamides; D011956:Receptors, Cell Surface; D014284:Triiodothyronine; D004958:Estradiol; D014212:Tretinoin; D003907:Dexamethasone; C051781:ICI 164384