Generation of superoxide anion by brain endothelial cell xanthine oxidase.
Bovine brain endothelial cells (EC) that were isolated and propagated in pure culture had increased (greater than 20-fold) levels of xanthine oxidase and xanthine dehydrogenase activity compared to whole brain homogenate. Brain EC also released superoxide anion (O2-) into the extracellular medium. Treatment of EC with tungsten decreased (P less than 0.05) both XO activity and O2- release. XO appears to be highly concentrated in cerebral vascular endothelium and may be an important source of O2-.
Terada
L S
LS
Webb-Waring Lung Institute, Denver, Colorado.
Willingham
I R
IR
Rosandich
M E
ME
Leff
J A
JA
Kindt
G W
GW
Repine
J E
JE
D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000201:enzymology; D002196:Capillaries / Q000201:enzymology; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D013481:Superoxides / Q000378:metabolism*; D014968:Xanthine Dehydrogenase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism*
D013481:Superoxides; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase