Transmitter regulation of voltage-dependent K+ channels expressed in Xenopus oocytes.
Voltage-dependent K+ channels (RBK1, RBK2 and RGK5) were co-expressed in Xenopus oocytes with 5-hydroxytryptamine (5-HT2) receptors. K+ currents measured 2-4 days later were inhibited by 5-HT (100 nM-10 microM, 20-30 s application) by up to 90%. The effect of 5-HT was mimicked by intracellular injection of Ins(1,4,5)P3. Increasing the Ca2+ concentration at the inner surface of excised membrane patches did not decrease the K+ current.
Kavanaugh
M P
MP
Vollum Institute, Oregon Health Sciences University, Portland 97201.
Christie
M J
MJ
Osborne
P B
PB
Busch
A E
AE
Shen
K Z
KZ
Wu
Y N
YN
Seeburg
P H
PH
Adelman
J P
JP
North
R A
RA
D000818:Animals; D002118:Calcium / Q000502:physiology; D003001:Cloning, Molecular; D004553:Electric Conductivity; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology; D008564:Membrane Potentials; D015288:Neurokinin A / Q000494:pharmacology; D009865:Oocytes; D011188:Potassium / Q000502:physiology; D015221:Potassium Channels / Q000502:physiology*; D051381:Rats; D012701:Serotonin / Q000494:pharmacology; D014982:Xenopus laevis
D015221:Potassium Channels; D012701:Serotonin; D015544:Inositol 1,4,5-Trisphosphate; D015288:Neurokinin A; D011188:Potassium; D002118:Calcium