Assignment of the 1H chemical shifts of glycogen.
Assignments of nearly all the 1H chemical shifts of glycogen are made by 2-D 1H-1H homonuclear and 13C-1H heteronuclear COSY. We demonstrated that it is possible to obtain well-resolved 2-D n.m.r. spectra for a large molecule like glycogen. The seven nonequivalent protons of the glucose residues in the alpha-(1----4)-linked chains, and of those at the nonreducing ends, were completely assigned. Distinct chemical shifts for H-1 and H-2 immediately adjacent to the alpha-(1----6) bonds at the branch points were also determined. Several modifications of previous 13C chemical shift assignments were made from the heteronuclear 2-D n.m.r. data.
Zang
L H
LH
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06510.
Howseman
A M
AM
Shulman
R G
RG
D000818:Animals; D002247:Carbon Isotopes; D003903:Deuterium; D017666:Deuterium Oxide; D005947:Glucose / Q000737:chemistry; D006003:Glycogen / Q000737:chemistry*; D006358:Hot Temperature; D007534:Isomaltose / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008320:Maltose / Q000737:chemistry; D011522:Protons; D011817:Rabbits; D014867:Water
D002247:Carbon Isotopes; D011522:Protons; D014867:Water; D007534:Isomaltose; D008320:Maltose; D006003:Glycogen; D003903:Deuterium; D005947:Glucose; D017666:Deuterium Oxide