Animal serum factor(s) causing adverse effects on RIAs of human enterovirus IgM.
The effects of animal sera used at various concentrations in dilution buffers for radioimmunoassays (RIAs) of human enterovirus-IgM were studied. The origin of the sera had no impact on the titres, but adverse effects on virus-specificity tests were noted when sera from some species were used. In attempts to block the binding of 35S-labelled virus by adding unlabelled virus, sera from cow, horse and lamb and from swine and man could usually not be used; instead of a blocking effect, an increase in bound labelled virus was noted in most cases. When fetal or newborn calf serum or sera from chicken were used, this phenomenon did not occur. The factor(s) causing the enhancement of virus binding could not be identified, but it was evident that it was not present in all sera from the same species and it was very probable that immunoglobulins were not involved.
Frisk
G
G
Department of Medical Virology, Biomedical Centre, University of Uppsala, Sweden.
Diderholm
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000737:chemistry*; D001790:Blood Physiological Phenomena; D002417:Cattle; D004770:Enterovirus / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D006736:Horses; D006801:Humans; D007075:Immunoglobulin M / Q000737:chemistry*; D011863:Radioimmunoassay; D012680:Sensitivity and Specificity; D012756:Sheep; D013552:Swine
D000914:Antibodies, Viral; D007075:Immunoglobulin M