Rotational mobility of high-affinity epidermal growth factor receptors on the surface of living A431 cells.
The rotational diffusion of epidermal growth factor (EGF) bound to its specific receptor on the surface of human carcinoma A431 cells was studied by means of time-resolved phosphorescence anisotropy measurements. The rotational mobility was measured on the total population of EGF receptors by using a saturating concentration of EGF conjugated with a phosphorescent label, erythrosin, or on the subpopulation of high-affinity EGF receptors by using a low concentration of labeled EGF. At 4 degrees C, the rotational correlation times for both the high-affinity and total (mostly low affinity) receptor populations were in the range of 60-100 microns. Elevation of the temperature to 37 degrees C resulted in a lengthening of the rotational correlation time of the total receptor population to 200-300 microns, confirming a previous study of receptor microaggregation. The high-affinity EGF receptors were completely immobilized at 37 degrees C (rotational correlation time greater than 500 microns). The data are consistent with a model involving association of the cytoskeleton with the high-affinity receptors at 37 degrees C, but not at 4 degrees C.
Zidovetzki
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R
Department of Biology, University of California, Riverside 92521.
Johnson
D A
DA
Arndt-Jovin
D J
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Jovin
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TM
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004058:Diffusion; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism*; D004923:Erythrosine / Q000378:metabolism; D005454:Fluorescence Polarization; D006801:Humans; D011940:Receptor Aggregation; D013696:Temperature; D014407:Tumor Cells, Cultured
D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D004923:Erythrosine