Induction of chromosome damage by restriction enzymes during mitosis.
Once electroporated into the nucleus of eukaryotic cells, restriction enzymes will bind at specific DNA sequences and cleave DNA to make double-strand breaks. These induced breaks can lead to chromosome aberrations and consequently offer one approach to determining the mechanism(s) of aberration formation. Because the higher-order structure of DNA in eukaryotic cells might influence the ability of restriction enzymes to locate their recognition sequence, bind, and cleave DNA, we have investigated whether enzymes will cut DNA during metaphase when the chromosomes are most condensed. Chinese hamster ovary cells synchronized in mitosis and treated with either AluI or Sau3AI showed few chromosome aberrations when held in mitosis for 1, 2, or 3 h after enzyme treatment. However, some disruption of chromosome morphology was seen, especially after exposure to Sau3AI. When cells were allowed to complete one cell cycle after enzyme treatment in the preceding mitosis, there was extensive chromosome damage, with the most abundant type of lesion being the interstitial deletion. It appears that restriction enzymes will cleave the highly condensed DNA in mitotic cells but that decondensation, DNA replication, and recondensation are required before the aberrations are manifested.
Morgan
W F
WF
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143-0750.
Valcarcel
E R
ER
Abella Columna
E
E
Winegar
R A
RA
Yates
B L
BL
D000818:Animals; D002869:Chromosome Aberrations; D004262:DNA Restriction Enzymes; D066298:In Vitro Techniques; D008938:Mitosis
D004262:DNA Restriction Enzymes