Transcriptional repression mediated by the WT1 Wilms tumor gene product.
The wt1 gene, a putative tumor suppressor gene located at the Wilms tumor (WT) locus on chromosome 11p13, encodes a zinc finger-containing protein that binds to the same DNA sequence as EGR-1, a mitogen-inducible immediate-early gene product that activates transcription. The transcriptional regulatory potential of WT1 has not been demonstrated. In transient transfection assays, the WT1 protein functioned as a repressor of transcription when bound to the EGR-1 site. The repression function was mapped to the glutamine- and proline-rich NH2-terminus of WT1; fusion of this domain to the zinc finger region of EGR-1 converted EGR-1 into a transcriptional repressor.
Madden
S L
SL
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Cook
D M
DM
Morris
J F
JF
Gashler
A
A
Sukhatme
V P
VP
Rauscher
F J
FJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002880:Chromosomes, Human, Pair 11; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D016147:Genes, Tumor Suppressor; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000235:genetics*; D014158:Transcription, Genetic; D009396:Wilms Tumor / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D004247:DNA