Interaction of a cellular 57-kilodalton protein with the internal translation initiation site of foot-and-mouth disease virus.
A cellular 57-kDa protein (p57) that binds specifically to the internal translation initiation site in the 5' untranslated region of foot-and-mouth disease virus RNA was detected in cell extracts of different mammalian species by UV cross-linking. The protein binds to two distinct sites of the translation control region which have as the only common sequence a UUUC motif. The first binding site consists of a conserved hairpin structure, whereas the second binding site contains an essential pyrimidine-rich region without obvious secondary structure. Competition experiments indicate that the complexes with the two binding sites were formed by a single p57 species. The protein binds also to the 5' untranslated region of other picornaviruses. Results from footprint analyses with foot-and-mouth disease RNA suggest the participation of additional cellular factors in the translation initiation complex.
Luz
N
N
Zentrum für Molekulare Biologie Heidelberg, University of Heidelberg, Germany.
Beck
E
E
D000818:Animals; D005537:Aphthovirus / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D003432:Cross-Linking Reagents; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000502:physiology*; D008961:Models, Structural; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D010442:Peptide Chain Initiation, Translational; D010849:Picornaviridae / Q000235:genetics / Q000502:physiology; D010957:Plasmids; D017955:Poliovirus / Q000235:genetics / Q000502:physiology; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D015183:Restriction Mapping; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D012367:RNA, Viral; D016601:RNA-Binding Proteins