Raf-1 is a potential substrate for mitogen-activated protein kinase in vivo.
MAP (mitogen-activated protein) kinase is shown to phosphorylate baculovirally expressed Raf-1 in vitro, generating one major tryptic phosphopeptide which co-migrated with a peptide from Raf-1 32P-labelled in situ. This peptide also undergoes an insulin-dependent increase in labelling. Thus the serine/threonine kinase Raf-1 may be a substrate for MAP kinase in vivo.
Anderson
N G
NG
Department of Internal Medicine, University of Virginia, Charlottesville 22908.
Li
P
P
Marsden
L A
LA
Williams
N
N
Roberts
T M
TM
Sturgill
T W
TW
D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002460:Cell Line; D018398:Homeodomain Proteins; D007313:Insecta; D010449:Peptide Mapping; D010748:Phosphopeptides / Q000032:analysis; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D013945:Thymoma; D013953:Thymus Neoplasms; D014162:Transfection
D018398:Homeodomain Proteins; D010748:Phosphopeptides; D011506:Proteins; D011994:Recombinant Proteins; C064658:RAG-1 protein; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases