Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit.
Multiple catalytic subunits of the Ca2+ and calmodulin (CaM)-dependent protein phosphatase (PrP) ("calcineurin" or PrP-2B) are derived from at least two structural genes, type 1 ("calcineurin A alpha") and type 2 ("calcineurin A beta "), each of which can produce alternatively spliced transcripts. To examine the possible linkage of these genes, we analyzed genomic DNA from human/hamster hybrid cell lines using probes of 122 base pairs that were designed to bind selectively to exon 3 of the open reading frame. In this region, the nucleotide sequence of the type 2 murine cDNA that we cloned was greater than 99% identical to the type 2 human cDNA but only 78% identical to the type 1 human cDNA. Hybridization to Southern blots containing DNA from all human chromosomes showed that gene 1 was found on chromosome 4, whereas gene 2 segregated to chromosome 10. These data suggest that expression of the two calcineurin genes is not physically linked.
Giri
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Section on Immunology, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD 20852.
Higuchi
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Kincaid
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D019703:Calcineurin; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D002879:Chromosomes, Human, Pair 10; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013950:Thymus Gland / Q000201:enzymology
D002148:Calmodulin-Binding Proteins; D046911:Macromolecular Substances; D004247:DNA; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases