Signal transduction pathways in the induction of HLA class I antigen expression on Huh 6 cells by interferon-gamma.
This study investigated the intracellular signal transduction regulating the appearance of HLA class I antigens on Huh 6 cells induced by interferon-gamma. The expression was blocked by a protein kinase C inhibitor, H-7, but not by a calmodulin antagonist, W-7, nor by a protein kinase A inhibitor, H-8, at low dose. The antigen expression was induced by a direct activator of protein kinase C, phorbol myristate acetate, but not by calcium ionophore A23187 nor an analog of cAMP, dbcAMP. Therefore, we concluded that protein kinase C is involved in the expression of HLA class I antigens on Huh 6 cells induced by interferon-gamma but Ca(2+)-calmodulin and cAMP are not.
Towata
T
T
First Department of Medicine, Osaka University Medical School, Japan.
Hayashi
N
N
Katayama
K
K
Takehara
T
T
Sasaki
Y
Y
Kasahara
A
A
Fusamoto
H
H
Kamada
T
T
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000001:Calcimycin / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D002460:Cell Line; D003563:Cyclic CMP / Q000031:analogs & derivatives / Q000494:pharmacology; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007546:Isoquinolines / Q000494:pharmacology; D008113:Liver Neoplasms / Q000276:immunology*; D010879:Piperazines / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*; D013449:Sulfonamides / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D002147:Calmodulin; D015395:Histocompatibility Antigens Class I; D007546:Isoquinolines; D010879:Piperazines; D013449:Sulfonamides; D003563:Cyclic CMP; D000001:Calcimycin; C053441:dibutyryl cyclic-3',5'-cytidine monophosphate; C017967:W 7; D007371:Interferon-gamma; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; D013755:Tetradecanoylphorbol Acetate