title: Difference in liver and serum malathion carboxylesterase and glucose-6-phosphatase in detecting carbon tetrachloride-induced liver damage in rats.
abstract: Microsome, cytosol and serum malathion carboxylesterase (MaCEst) activity was assessed in rats after single i.p. administration of carbon tetrachloride (CCl4) in doses ranging from 0.05 to 1 ml kg-1. MaCEst activities were compared with those of glucose-6-phosphatase (G6-Pase) as an indicator of endoplasmic reticulum damage and serum glutamate dehydrogenase (GLDH) and sorbitol dehydrogenase (SHD) as indicators of liver cytolysis. The data showed a dose-dependent increase in GLDH and SDH serum activities (175% and 68%) from 0.05 ml kg-1; an increase in serum G6-Pase (31%) and a decrease in microsomal G6-Pase (38%) was apparent only after 0.5 or 1.0 ml kg-1 doses. MaCEst activity was unaffected. The results demonstrate that, under these experimental conditions, serum and subcellular measurements of MaCEst activity failed to reveal the liver toxicity of CCl4.
authors.affiliation: Institut National de Recherche et de Sécurité, Vandoeuvre, France.
authors.forename: M T
authors.initials: MT
authors.lastname: Brondeau
authors.forename: C
authors.initials: C
authors.lastname: Coulais
authors.forename: J
authors.initials: J
authors.lastname: de Ceaurriz
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002265:Carboxylic Ester Hydrolases / Q000032:analysis* / Q000097:blood; D005952:Glucose-6-Phosphatase / Q000032:analysis* / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002251:Carbon Tetrachloride; D002265:Carboxylic Ester Hydrolases; C021528:malathion esterases; D005952:Glucose-6-Phosphatase