[Investigation of anti-hepatitis C virus antibody in healthy blood donors in China: enzyme immunoassay and recombinant immunoblot].
281 serum samples from Chinese healthy blood donors (HBsAg negative and ALT normal) were tested for anti-HCV antibodies with ortho-HCV ELISA test system and Abbott HCV EIA diagnostic kits. The results were further confirmed with recombinant immunoblot assay (RIBA). Our research showed that the prevalence of anti-HCV antibody in this group was 2.1% (6/281), being higher than that in other countries and regions. It indicated that great attention should be paid to HCV infection in China. The reason of dark groundback and high false positive reactivity produced by the ELISA was discussed. So, the positive reactivity produced by ELISA should be confirmed by other specific method such as RIBA. Reexamination of the 281 serum samples with HBsAg AUSRIA II showed that HBsAg was positive in 7 and HBVDNA positive in one of them.
Zhang
Y Y
YY
Clinical Immunology Research Unit, Tongji Hospital, Tongji Medical University, Wuhan.
Yan
X B
XB
Li
F H
FH
D001782:Blood Donors; D002681:China / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000453:epidemiology; D006801:Humans; D015151:Immunoblotting; D008297:Male; D016036:Seroepidemiologic Studies
D006508:Hepatitis Antibodies