Cytochemical stainings in acute promyelocytic leukemia: chloroacetate esterase reaction as a prognostic index.
Cytochemical studies including peroxidase, sudan black B and esterases were used for staining peripheral blood and bone marrow smears from 42 patients with acute promyelocytic leukemia. The most sensitive methods were sudan black B (mean 98%, range 81-100%) and peroxidase (mean 92% range 70-100%). Naphthol AS-D chloroacetate esterase activity was less sensitive and was positive in only 49.4 per cent (range 2-100%). All of the population of leukemic cells contained less than 3 per cent of alpha-naphthyl acetate esterase staining. For stability tests of the storage specimens compared to fresh stains, there was no difference in naphthol AS-D chloroacetate esterase (mean 45% vs 49% P greater than 0.7) and sudan black B (mean 74% vs 98% P greater than 0.3), but the enzyme activity was significantly decreased in peroxidase staining (mean 42% vs 92% P greater than 0.05). When the patients were divided into 2 groups according to the degree of AS-D chloroacetate esterase activity, those with lower activity had a higher number of white blood cells, promyelocytes and shorter survival compared to those with higher activity. Therefore, naphthol AS-D chloroacetate esterase may be useful as a prognostic index.
Chinprasertsuk
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Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Piankijagum
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A
Issaragrisil
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S
D000293:Adolescent; D000328:Adult; D000368:Aged; D001391:Azo Compounds; D001856:Bone Marrow Examination; D004950:Esterases; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000097:blood* / Q000453:epidemiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009281:Naphthalenes; D009195:Peroxidase; D012680:Sensitivity and Specificity
D001391:Azo Compounds; D009281:Naphthalenes; C016118:Sudan Black B; D009195:Peroxidase; D004950:Esterases