Investigation of sites of pseudorabies virus latency, using polymerase chain reaction.
Pseudorabies virus (PRV) latency was investigated, using polymerase chain reaction (PCR). A PCR protocol was developed that specifically amplified a 217-base pair sequence within the gene encoding the essential glycoprotein gp50 of PRV. Using this PCR procedure, the gp50 sequence was amplified from tissues of pigs infected with various doses of PRV (Becker strain). At postinoculation day 64, viral isolation was performed on nasal swab specimens and homogenates of tonsils and trigeminal nerve ganglia obtained from 11 PRV-convalescent, seropositive pigs. Results were negative in all cases. By use of PCR, 11 of 11 pigs had PRV-positive trigeminal nerve ganglia and brain stem, 10 of 11 pigs had PRV-positive tonsils, and 9 of 11 pigs had PRV-positive olfactory bulbs.
Wheeler
J G
JG
Department of Veterinary Sciences, University of Nebraska-Lincoln 68586-0905.
Osorio
F A
FA
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001933:Brain Stem / Q000382:microbiology; D015342:DNA Probes / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry; D005784:Gene Amplification; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009830:Olfactory Bulb / Q000382:microbiology; D014066:Palatine Tonsil / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011557:Pseudorabies / Q000382:microbiology*; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D012668:Trigeminal Ganglion / Q000382:microbiology; D014759:Viral Envelope Proteins / Q000235:genetics*
D015342:DNA Probes; D004279:DNA, Viral; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins