Induction of amyloid precursor protein mRNA after heat shock in cultured human lymphoblastoid cells.
In an attempt to examine a possible relationship between heat shock stress and an induction of amyloid precursor protein, cultured lymphoblastoid cells established from 12 human subjects were treated with heat shock at 42 degrees C for 30 min. The levels of mRNA for amyloid precursor protein (APP), heat shock protein (HSP) 70, and actin were examined by Northern blot at 1, 3, 8, 24, and 48 h after the heat shock treatment. HSP70 mRNA was induced at 1 and 3 h, and became undetectable again by 8 h. APP mRNA was also induced at 3 and 8 h, and recovered to the steady level by 48 h. No induction was observed in actin mRNA. These results indicate that APP mRNA is induced by heat shock treatment after the induction of HSP70 mRNA, suggesting a role of heat shock response in an induction of APP.
Abe
K
K
Department of Neurology, Tohoku University School of Medicine, Sendai Japan.
St George-Hyslop
P H
PH
Tanzi
R E
RE
Kogure
K
K
D000199:Actins / Q000235:genetics; D000544:Alzheimer Disease / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000235:genetics*; D016564:Amyloid beta-Protein Precursor; D015152:Blotting, Northern; D002460:Cell Line; D002472:Cell Transformation, Viral; D005260:Female; D006360:Heat-Shock Proteins / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D008214:Lymphocytes; D008297:Male; D008875:Middle Aged; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification
D000199:Actins; D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D006360:Heat-Shock Proteins; D011498:Protein Precursors; D012333:RNA, Messenger