Stimulation of DNA synthesis in Jurkat cells by synergistic action between adenine and guanine nucleotides.
P2-purinoceptor agonists stimulated the DNA synthesis of Jurkat cells via a pathway independent of cAMP and intracellular free calcium. The response was greatly enhanced by the synergistic action between adenine and guanine nucleotides, suggesting that binding sites of these nucleotides are different from each other, and the proliferation is stimulated by a novel interaction between adenine and guanine nucleotide receptors. The stimulatory effects of P2-agonists on proliferation was completely abolished by cholera toxin and attenuated by pertussis toxin, which suggests that substrates for cholera toxin and pertussis toxin are involved in the proliferative pathways associated with P2-purinoceptors.
Tokumitsu
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Y
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Yanagawa
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Y
Nomura
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Y
D000227:Adenine Nucleotides / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006150:Guanine Nucleotides / Q000494:pharmacology*; D006801:Humans; D037342:Pertussis Toxin; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D000227:Adenine Nucleotides; D006150:Guanine Nucleotides; D010566:Virulence Factors, Bordetella; D004247:DNA; D002772:Cholera Toxin; D000242:Cyclic AMP; D037342:Pertussis Toxin; D002118:Calcium