title: Determination of infectious bursal disease virus titration and neutralization endpoints using fluorogenic staining.
abstract: An automated method for determining infectious bursal disease virus (IBDV) titration and neutralization endpoints is described. The method employs the fluorogenic ester carboxyfluorescein-diacetate (CFDA) to stain cell monolayers in 96-well plates and a fluorescence-concentration analyzer. Titration results are compared with immunofluorescence and plaque assay titers. Virus-neutralization endpoint determination is objective, and the endpoints of replicate tests were equivalent or within one dilution of variability. Tests can be automatically screened as any percentage of a positive control or any standard deviation from a negative control.
authors.affiliation: Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville 72701.
authors.forename: G R
authors.initials: GR
authors.lastname: Bayyari
authors.forename: J K
authors.initials: JK
authors.lastname: Skeeles
authors.forename: J D
authors.initials: JD
authors.lastname: Story
authors.forename: M F
authors.initials: MF
authors.lastname: Slavik
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D007243:Infectious bursal disease virus / Q000254:growth & development* / Q000276:immunology; D007668:Kidney / Q000166:cytology; D009500:Neutralization Tests; D013047:Specific Pathogen-Free Organisms; D010948:Viral Plaque Assay
chemical_list: D005452:Fluoresceins; C027780:carboxyfluoresceindiacetate