title: Nucleotide sequence of IS402 from Pseudomonas cepacia.
abstract: IS402, a transposable gene-activating element isolated on the basis of its ability to increase expression of the Tn1 bla gene in Pseudomonas cepacia, was cloned from pTGL52 into the vector, pBluescript KS+, and its nucleotide (nt) sequence was determined. This 914-bp element had terminal inverted repeats of 17 bp with a single mismatch, and upon insertion into Tn1 generated a direct target duplication of 3 bp. Comparison of its nt sequence with the GenBank and EMBL databases indicated that IS402 is unrelated to previously described bacterial IS elements.
authors.affiliation: Department of Microbiology, University of Massachusetts, Amherst 01003.
authors.forename: A A
authors.initials: AA
authors.lastname: Ferrante
authors.forename: T G
authors.initials: TG
authors.lastname: Lessie
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements