Photoinactivation of viruses in human fresh plasma by phenothiazine dyes in combination with visible light.
We developed a photodynamic method to inactivate viruses in human fresh plasma. Single plasma bags were illuminated with visible light in the presence of low doses of phenothiazine dyes like methylene blue or toluidine blue. By this treatment the infectivity of different enveloped viruses including the causative agent of AIDS, HIV-1, was completely removable from the plasma. Non enveloped viruses, however, proved to be more stable. The activities of clotting factors and other plasma proteins were only slightly decreased. There was no indication that the procedure led to important structural modifications of plasma proteins. The dyes are photodynamically active at concentrations much lower than those at which they are therapeutically used as antidots in the treatment of methemoglobinemia.
Lambrecht
B
B
DRK-Blutspendedienst Niedersachsen, Institut Springe, BRD.
Mohr
H
H
Knüver-Hopf
J
J
Schmitt
H
H
D003588:Cytopathogenic Effect, Viral / Q000187:drug effects* / Q000528:radiation effects; D015497:HIV-1 / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D007122:Immunoelectrophoresis; D008027:Light; D008751:Methylene Blue / Q000494:pharmacology*; D010949:Plasma / Q000382:microbiology*; D018139:Simplexvirus / Q000187:drug effects; D014048:Tolonium Chloride / Q000494:pharmacology*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects; D014775:Virus Activation
D014048:Tolonium Chloride; D008751:Methylene Blue