title: Molecular biology of circulatory shock: IV. Translation and secretion of HEP G2 cell proteins are independently attenuated during heat shock.
abstract: In previous studies we have shown evidence of heat shock gene transcription in pig liver after cardiogenic shock (Buchman et al: Surgery 108:559, 1990). To study the effect of heat shock on synthesis, secretion, and glycosylation of liver cell proteins, a human hepatoblastoma cell line (Hep G2) was used as a model system. Cells were thermally stressed and incorporation of radioactive amino acids or carbohydrates was studied. Heat shock caused a tenfold decrease in the rate of overall protein synthesis; secretion was attenuated to a lesser extent; glycosylation was spared. Heat shock gene expression is associated with a precipitous and sustained decline in the synthesis and secretion of constitutive proteins which may be critical to recovery from circulatory shock.
authors.affiliation: Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
authors.forename: A
authors.initials: A
authors.lastname: De Maio
authors.forename: T G
authors.initials: TG
authors.lastname: Buchman
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D006031:Glycosylation; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D008967:Molecular Biology; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D012770:Shock, Cardiogenic / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D006360:Heat-Shock Proteins; D009363:Neoplasm Proteins