title: Stress-induced heat shock protein 70 expression in adrenal cortex: an adrenocorticotropic hormone-sensitive, age-dependent response.
abstract: The induction of heat shock proteins (HSP) by cellular stress and the activation of the hypothalamic-pituitary-adrenal axis by physiologic stress are biological responses that aid in the maintenance of cellular and organismal homeostasis, respectively. In this report, restraint stress, known to activate the hypothalamic-pituitary-adrenal axis, is shown to induce expression of HSP70 mRNA selectively in the adrenal cortex of the rat. Restraint-induced HSP70 expression in the adrenals is rapid and is preceded by the activation of a protein factor capable of binding to the heat shock transcriptional control element. The ability of restraint to induce HSP70 expression in the adrenal is virtually eliminated in hypophysectomized rats but can be restored by the exogenous administration of adrenocorticotropic hormone. The magnitude of this induction declines as a function of increasing age, which may contribute to a reduced stress tolerance by aged animals. These results support a role for HSP70 in the physiologic stress response mediated by the hypothalamic-pituitary-adrenal axis.
authors.affiliation: National Institute on Aging, Gerontology Research Center, Baltimore, MD 21224.
authors.forename: M J
authors.initials: MJ
authors.lastname: Blake
authors.forename: R
authors.initials: R
authors.lastname: Udelsman
authors.forename: G J
authors.initials: GJ
authors.lastname: Feulner
authors.forename: D D
authors.initials: DD
authors.lastname: Norton
authors.forename: N J
authors.initials: NJ
authors.lastname: Holbrook
mesh_terms: D000302:Adrenal Cortex / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000502:physiology; D000375:Aging; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004268:DNA-Binding Proteins / Q000378:metabolism; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000378:metabolism*; D007016:Hypophysectomy; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D010913:Pituitary-Adrenal System / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D012045:Regulatory Sequences, Nucleic Acid; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D009841:Oligonucleotides; D012333:RNA, Messenger; D014157:Transcription Factors; D000324:Adrenocorticotropic Hormone