title: Inflammation-induced changes in expression and glycosylation of genetic variants of alpha 1-acid glycoprotein. Studies with human sera, primary cultures of human hepatocytes and transgenic mice.
abstract: The relative occurrence of genetic variants of human alpha 1-acid glycoprotein (AGP) in relation to changes in glycosylation was studied in sera of patients with burn injury, media of cytokine-treated primary cultures of human hepatocytes and Hep 3B cells, and sera of transgenic mice expressing the human AGP-A gene. It is concluded (i) that the glycosylation of AGP was not dependent on its genetic expression and (ii) that both the variants determined by the AGP-A gene as well as by the AGP-B/B' genes are increased after inflammation or treatment with interleukins 1 and 6.
authors.affiliation: Department of Medical Chemistry, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: W
authors.initials: W
authors.lastname: van Dijk
authors.forename: O
authors.initials: O
authors.lastname: Pos
authors.forename: M E
authors.initials: ME
authors.lastname: van der Stelt
authors.forename: H J
authors.initials: HJ
authors.lastname: Moshage
authors.forename: S H
authors.initials: SH
authors.lastname: Yap
authors.forename: L
authors.initials: L
authors.lastname: Dente
authors.forename: P
authors.initials: P
authors.lastname: Baumann
authors.forename: C B
authors.initials: CB
authors.lastname: Eap
mesh_terms: D000818:Animals; D002056:Burns / Q000097:blood / Q000503:physiopathology; D006528:Carcinoma, Hepatocellular; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D015870:Gene Expression; D014644:Genetic Variation; D006031:Glycosylation; D006801:Humans; D007249:Inflammation; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008113:Liver Neoplasms; D051379:Mice; D008822:Mice, Transgenic; D009961:Orosomucoid / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D016207:Cytokines; D009961:Orosomucoid