Vimentin is hyperphosphorylated in primary human fibroblasts treated with okadaic acid.
Okadaic acid and dinophysistoxin-1 (35-methylokadaic acid) induced hyperphosphorylation of a 58 kDa protein in primary human fibroblasts, due to inhibition of protein phosphatase 1 and 2A activities. The protein was present in the nuclear and cytosolic fractions. Its pI was 5.3. The hyperphosphorylated protein reacted with monoclonal and polyclonal anti-vimentin antibodies, but not with anti-nucleolin antibody. Phosphorylation of vimentin was stimulated in vitro by dinophysistoxin-1 dose-dependently in the presence of protein phosphatase 2A and protein kinases.
Yatsunami
J
J
Cancer Prevention Division, National Cancer Center Research Institute, Tokyo, Japan.
Fujiki
H
H
Suganuma
M
M
Yoshizawa
S
S
Eriksson
J E
JE
Olson
M O
MO
Goldman
R D
RD
D015153:Blotting, Western; D004988:Ethers, Cyclic / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008387:Marine Toxins / Q000494:pharmacology; D008970:Molecular Weight; D019319:Okadaic Acid / Q000031:analogs & derivatives; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2; D011714:Pyrans / Q000494:pharmacology; D014746:Vimentin / Q000302:isolation & purification / Q000378:metabolism*
D004988:Ethers, Cyclic; D008387:Marine Toxins; D011714:Pyrans; D014746:Vimentin; D019319:Okadaic Acid; C051904:dinophysistoxin 1; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2