Experimental glaucoma significantly decreases atrial natriuretic factor (ANF) receptors in the ciliary processes of the rabbit eye.
ANF binding sites were analysed in the ciliary processes of rabbits with unilateral experimental glaucoma which had been induced by injecting alpha-chymotrypsin into the posterior chamber of the right eyes. The intraocular pressure (IOP) of glaucomatous eyes was significantly greater (28.4 +/- 4 mmHg) than that of normotensive control eyes (13.1 +/- 1.4 mmHg, mean +/- S.E.M., n = 23, P less than 0.05). ANF concentrations in aqueous humour and the ciliary processes were significantly higher in glaucomatous eyes (91 +/- 2 pg ml-1 and 30.4 +/- 4.2 pg g-1 wet weight) than in normal eyes (3.1 +/- 2.2 pg ml-1 and 10.2 +/- 2.7 pg g-1 wet weight, respectively, n = 6, P less than 0.01). The number of ANF-binding sites (Bmax) in the ciliary processes of glaucomatous rabbit eyes was significantly decreased in comparison to the controls (24 +/- 4 vs. 13 +/- 3 fmol mg-1 protein, n = 10, P less than 0.05). These data suggest that ANF receptors in the ciliary processes are down-regulated and that ANF may play an important role in the pathophysiology of experimental glaucoma.
Fernandez-Durango
R
R
Departamento de Medicina Interna III, Ocular Hospital Universitario de San Carlos, Ciudad Universitaria, Madrid, Spain.
Ramirez
J M
JM
Trivino
A
A
Sanchez
D
D
Paraiso
P
P
Garcia De Lacoba
M
M
Ramirez
A
A
Salazar
J J
JJ
Fernandez-Cruz
A
A
Gutkowska
J
J
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001665:Binding Sites; D002918:Chymotrypsin; D002924:Ciliary Body / Q000737:chemistry*; D005901:Glaucoma / Q000139:chemically induced / Q000378:metabolism*; D007429:Intraocular Pressure / Q000502:physiology; D011817:Rabbits; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000032:analysis*
D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D002918:Chymotrypsin; D017461:Receptors, Atrial Natriuretic Factor