Quantitative analysis of nonoxynol-9 in blood.
At present, there is little data on the level of nonoxynol-9 (N-9) in blood. Using routine methods, the rate of recovery for N-9 in blood has been confirmed to be very low. A method determining N-9 levels in urine has been found but obviously a more efficient method for determining N-9 levels in blood and urine is still needed. Here, a method of determining N-9, a nonionic surfactant spermicide, in blood is presented. The analytical procedure is a simple and convenient method. Using HPLC, UV detector, ODS reversed-phase column, methanol-water mobile phase, NaCl salting-out and two extractions with benzene, a rate of recovery for N-9 of 93 +/- 4% has been obtained.
Yang
J T
JT
Tianjin Municipal Research Institute for Family Planning, China.
Zhao
Z W
ZW
D000282:Administration, Intravaginal; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003271:Contraceptive Agents, Female / Q000008:administration & dosage / Q000032:analysis*; D005260:Female; D006801:Humans; D017137:Nonoxynol; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000032:analysis*; D012965:Sodium Chloride; D013089:Spermatocidal Agents / Q000008:administration & dosage / Q000032:analysis*; D013488:Suppositories; D014466:Ultraviolet Rays
D003271:Contraceptive Agents, Female; D013089:Spermatocidal Agents; D013488:Suppositories; D017137:Nonoxynol; D011092:Polyethylene Glycols; D012965:Sodium Chloride