Identification and DNA sequence of a new Bacteroides fragilis insertion sequence-like element.
A new Bacteroides fragilis insertion sequence (IS)-like element has been identified, cloned, and sequenced. The element is 1598 base pairs in length. It is flanked by a 15-base pair imperfect inverted repeat and contains a large open reading frame which could encode a 430 amino acid protein. There is an 8-base pair duplication of genomic DNA sequences at the site of integration. One copy of the IS-like element is integrated within the 5' upstream sequence of the metallo-beta-lactamase gene ccrA, cloned from B. fragilis TAL3636. The IS-like element is integrated 19 bp upstream of the predicted initiation codon and, therefore, probably provides the transcriptional start signals for the CcrA gene.
Rasmussen
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BA
Molecular Biology Section, American Cyanamid Company, Pearl River, New York 10965.
Kovacs
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E
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001441:Bacteroides fragilis / Q000235:genetics*; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D001618:beta-Lactamases
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D001618:beta-Lactamases; C063912:carbapenemase