title: Fluorescent and photoaffinity labeling probes for retinoic acid receptors.
abstract: A fluorescent probe for retinoid receptors (RARs) was designed and prepared. The probe consists of a retinoid moiety and a dansyl moiety, i.e., 2-[3-(5-dimethylaminonaphthalene-1-sulfonyl)- aminopropyl-1-oxy]-4-[(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2- naphthalenyl)carboxamido]benzoic acid: DAM-3. DAM-3 specifically bound RARs. Additionally, a photoreactive RAR fluorescent probe was designed and prepared, i.e., 2-[3-(5-azidonaphthalene- 1-sulfonyl)aminopropyl-1-oxy]-4-[(5,6,7,8-tetrahydro-5,5,8,8- tetramethyl-2-naphthalenyl)carboxamido]benzoic acid (ADAM-3). ADAM-3 irreversibly and specifically bound RARs using ultraviolet irradiation.
authors.affiliation: Institute of Applied Microbiology, University of Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Shimazawa
authors.forename: R
authors.initials: R
authors.lastname: Sanda
authors.forename: H
authors.initials: H
authors.lastname: Mizoguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: S
authors.initials: S
authors.lastname: Iwasaki
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Kagechika
authors.forename: K
authors.initials: K
authors.lastname: Shudo
mesh_terms: D000345:Affinity Labels / Q000138:chemical synthesis*; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D003619:Dansyl Compounds; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D018168:Receptors, Retinoic Acid; D013329:Structure-Activity Relationship; D014212:Tretinoin / Q000378:metabolism*
chemical_list: D000345:Affinity Labels; D002352:Carrier Proteins; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D018168:Receptors, Retinoic Acid; D014212:Tretinoin