Measurement of aromatisation by a urine technique suitable for the evaluation of aromatase inhibitors in vivo.
By modification of a recently developed method for separation of radio-labelled urinary oestrogens we were able to separate oestrogen metabolites and measure their isotope ratios in urine following injections of [3H]delta 4-androstenedione and [14C]oestrone. This method provides a useful tool for studying in vivo aromatisation of delta 4-androstenedione into oestrone in breast cancer patients before and during treatment with aromatase inhibitors.
Jacobs
S
S
Department of Academic Biochemistry, Royal Marsden Hospital, London, England.
Lønning
P E
PE
Haynes
B
B
Griggs
L
L
Dowsett
M
M
D000616:Aminoglutethimide / Q000627:therapeutic use; D000735:Androstenedione / Q000378:metabolism; D001141:Aromatase / Q000378:metabolism*; D047072:Aromatase Inhibitors; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D004967:Estrogens / Q000652:urine*; D004970:Estrone / Q000096:biosynthesis; D005260:Female; D006801:Humans
D047072:Aromatase Inhibitors; D004967:Estrogens; D000616:Aminoglutethimide; D004970:Estrone; D000735:Androstenedione; D001141:Aromatase