Dibutyryl-cAMP induces SNAP-25 translocation into the neurites in PC12.
SNAP-25 immunoreactivity was translocated into the endings of the processes induced in PC12 cells by dibutyryl-cAMP-treatment. Conversely, the protein was not present in the endings of the processes seen after NGF-treatment unless dibutyryl-cAMP was used simultaneously. This redistribution of SNAP-25 immunoreactivity appeared to be dependent upon new protein synthesis. Finally, dibutyryl-cAMP was capable of inducing SNAP-25 expression.
Sanna
P P
PP
Department of Neuropharmacology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Bloom
F E
FE
Wilson
M C
MC
D000310:Adrenal Gland Neoplasms / Q000503:physiopathology; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation; D002460:Cell Line; D008565:Membrane Proteins; D009414:Nerve Growth Factors / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010673:Pheochromocytoma / Q000503:physiopathology; D050825:Synaptosomal-Associated Protein 25
D008565:Membrane Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D050825:Synaptosomal-Associated Protein 25; D003994:Bucladesine