[Participation of protein kinase C in hormonal regulation of pepsin secretion in the rat stomach].
It was shown that pentagastrin (0.5 micrograms/100 g of body mass) increases the activity of Ca2+ and phospholipid-dependent protein kinase C in the membrane fraction of rat gastric mucosa cells. This effect of pentagastrin is accompanied by a decrease of the protein kinase C activity in the cytosolic fraction. Chromatography of the membrane fraction revealed an additional peak of the enzyme activity. Analysis of isolated gastric mucosa cells demonstrated that pentagastrin (10(-8)-10(-6) M) (but not 10(-4) M histamine) added to the incubation mixture increased the protein kinase C concentration in the membranes. The pentagastrin effect was directly correlated with the amount of pepsin-producing chief cells in the cellular pools. Carbacholine, another well-known pepsin secretion stimulator, was able to activate, similar to pentagastrin, the protein kinase C activity. It is concluded that protein kinase C plays a prominent role in hormonal regulation of the chief gastric cell function.
Sogumbaev
Zh K
ZhK
Tairov
M M
MM
Bersimbaev
R I
RI
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D002853:Chromatography, Liquid; D005753:Gastric Mucosa / Q000187:drug effects / Q000201:enzymology*; D006632:Histamine / Q000494:pharmacology; D006728:Hormones / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D010418:Pentagastrin / Q000494:pharmacology; D010434:Pepsin A / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D006728:Hormones; D010716:Phosphatidylinositols; D006632:Histamine; D002217:Carbachol; D011493:Protein Kinase C; D010434:Pepsin A; D010418:Pentagastrin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium
Uchastie proteinkinaszy C v gormonal'noĭ reguliatsii sekretsii pepsinav zheludke krys.