Ultrastructural alterations of the myocardium induced by doxorubicin. A scanning electron microscopic study.
Morphologic changes in Doxorubicin (DXR)-induced cardiomyopathy are characterized by marked dilatation of the sarcoplasmic reticulum (SR). DXR was administered to New Zealand White rabbits for 5 or 8 weeks and the three-dimensional structure of the sarcotubular system in cardiac muscle cells from each rabbit was examined under a field-emission type scanning electron microscope (SEM) after removal of cytoplasmic matrices by the osmium-DMSO-osmium procedure. Five weeks after the initial injection of DXR, partial dilatation of the SR and damaged mitochondria with lysis of cristae were observed three-dimensionally. After 8 weeks, the three-dimensional structure of the SR showed extensive spherical ballooning which could be seen clearly in bold relief. Thus, we could directly visualize structural alterations of the sarcotubular system in DXR-induced cardiomyopathy using the SEM.
Iwasaki
T
T
Second Department of Internal Medicine, Gunma University School of Medicine, Japan.
Suzuki
T
T
D000818:Animals; D009202:Cardiomyopathies / Q000139:chemically induced / Q000473:pathology*; D004121:Dimethyl Sulfoxide; D004317:Doxorubicin / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D008297:Male; D008855:Microscopy, Electron, Scanning; D008929:Mitochondria, Heart / Q000648:ultrastructure; D008933:Mitochondrial Swelling; D009206:Myocardium / Q000648:ultrastructure*; D009210:Myofibrils / Q000648:ultrastructure; D009992:Osmium; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure
D009992:Osmium; D004317:Doxorubicin; D004121:Dimethyl Sulfoxide