title: Glycogen accumulation and histological changes in the livers of lambs with alveld and experimental sporidesmin intoxication.
abstract: Alveld is a hepatogenous photosensitization disease seen in lambs grazing Narthecium ossifragum pastures in Norway. Mycotoxins, possibly sporidesmin, have been suspected to cause the liver damage in alvled as in facial eczema. The histological changes in the liver of alveld cases and in lambs photosensitized after experimental sporidesmin intoxication were compared. The liver damage, characterized by necrosis in single centrilobular hepatocytes, was of the same type in both conditions. Minor to moderate portal fibroplasia and bile duct proliferation were almost always present. Accumulated glycogen was seen in hepatocytes in the centrilobular areas. This was significantly correlated to the enzymatically measured glycogen content and there was good correlation between parenchymal damage and glycogen accumulation. The glucose-6-phosphatase and glycogen phosphorylase activities were normal. These findings indicate that parenchymal damage, rather than obstruction of the bile ducts, caused the retention of phylloerythrin both in alveld cases and in experimentally sporidesmin-intoxicated lambs. The accumulation of glycogen could not be explained.
authors.affiliation: Department of Large Animal Clinical Sciences, Norwegian College of Veterinary Medicine, Oslo.
authors.forename: A
authors.initials: A
authors.lastname: Flåøyen
authors.forename: B
authors.initials: B
authors.lastname: Borrebaek
authors.forename: K
authors.initials: K
authors.lastname: Nordstoga
mesh_terms: D000818:Animals; D005260:Female; D005952:Glucose-6-Phosphatase / Q000032:analysis; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008112:Liver Glycogen / Q000032:analysis; D008297:Male; D010787:Photosensitivity Disorders / Q000139:chemically induced / Q000473:pathology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000139:chemically induced / Q000473:pathology*; D013173:Sporidesmins / Q000506:poisoning*
chemical_list: D008112:Liver Glycogen; D013173:Sporidesmins; D005952:Glucose-6-Phosphatase; C001873:sporidesmin