Isolation and identification of hemin as an endogenous Na+/K(+)-ATPase inhibitor from porcine blood cells.
A substance which is a potent inhibitor of Na+/K(+)-ATPase activity and competitively displaces [3H]ouabain binding to this enzyme was isolated from porcine blood cells. From its chemical and physiochemical properties, this activity was identified as hemin (chloroprotohemin IX). Hemin showed a dose dependent curve for Na+/K(+)-ATPase inhibitory activity similar to that of ouabain and displaced [3H]ouabain binding as potent as 1/100 of ouabain itself.
Yasuhara
T
T
Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd., Japan.
Mori
M
M
Wakamatsu
K
K
Kubo
K
K
D000818:Animals; D001667:Binding, Competitive; D001794:Blood Pressure / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000097:blood*; D006427:Hemin / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D010042:Ouabain / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013552:Swine
D004791:Enzyme Inhibitors; C069763:chloroprotohemin IX; D010042:Ouabain; D006427:Hemin; D000254:Sodium-Potassium-Exchanging ATPase