title: After microinjection hemimethylated DNA is converted into symmetrically methylated DNA before DNA replication.
abstract: In this investigation we analysed the maintenance methylation activity of the mammalian cell DNA methyltransferase by microinjection of hemimethylated HSV-tk DNA into thymidine kinase-negative rat 2 cells. We found that the hemimethylated DNA was efficiently converted into symmetrical methylated molecules before DNA replication. Furthermore, integration of the trans-DNA into the host genome is an early event after gene transfer.
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freien Universitt Berlin, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Sandberg
authors.forename: E
authors.initials: E
authors.lastname: Guhl
authors.forename: M
authors.initials: M
authors.lastname: Graessmann
authors.forename: A
authors.initials: A
authors.lastname: Graessmann
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000008:administration & dosage / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000008:administration & dosage / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008745:Methylation; D008845:Microinjections; D051381:Rats; D015183:Restriction Mapping; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D013937:Thymidine Kinase