title: Differential inactivation of surrogate viruses with merocyanine 540.
abstract: Bacteriophages may be useful as surrogates for animal viruses when the virucidal properties of different photosensitizing compounds are initially investigated. We studied photoinactivation of four bacteriophages, phi X174, T7, PRD1, and phi 6, by the dye merocyanine 540 (MC540) (15 micrograms/mL). Merocyanine 540 (MC540) should be most effective with lipid-containing viruses, since it is primarily lipophilic (but also binds to proteins). Two of the phages, PRD1 and phi 6 contain lipid, with only phi 6 having an external lipoprotein envelope. Filtered radiation (450-600 nm) from a 750 W projector was used at 16-100 W/m2. The survival curves of the different viruses clearly demonstrated different levels of sensitivity to photoinactivation by MC540, with phi 6 (Do = 1.5 kJ/m2) being the most sensitive, followed by T7 (21-fold less sensitive). While both PRD1 and phi 6 have lipid components, only phi 6 was photoinactivated by MC540. Thus the internal lipid components of PRD1 were not sufficient to allow photoinactivation by this dye, at fluences up to 300 kJ/m2. For comparison, we also photoinactivated Herpes simplex virus (Do = 0.053 kJ/m2) and found it to be 28-fold more sensitive than phi 6 to photoinactivation by the same concentration of MC540. Thus phi 6 may be used as a surrogate for enveloped human viruses for photoinactivation by lipophilic dyes, but the results may only be useful qualitatively.
authors.affiliation: Center for Devices and Radiological Health, FDA, Rockville, MD 20857.
authors.forename: C D
authors.initials: CD
authors.lastname: Lytle
authors.forename: A P
authors.initials: AP
authors.lastname: Budacz
authors.forename: E
authors.initials: E
authors.lastname: Keville
authors.forename: S A
authors.initials: SA
authors.lastname: Miller
authors.forename: K N
authors.initials: KN
authors.lastname: Prodouz
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001435:Bacteriophages / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects; D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D008027:Light; D011744:Pyrimidinones / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D018139:Simplexvirus / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects
chemical_list: D000998:Antiviral Agents; D011744:Pyrimidinones; D011838:Radiation-Sensitizing Agents; C003954:merocyanine dye