title: DNase protection analysis of the stable synaptic complexes involved in Mu transposition.
abstract: Several critical steps in phage Mu transposition involve specialized protein-DNA complexes. Cleavage of Mu donor DNA by MuA protein leads to the formation of the stable cleaved donor complex (CDC), in which the two Mu DNA ends are held together by MuA. In the subsequent strand-transfer reaction the CDC attacks a target DNA to generate the strand-transfer complex, in which the donor and the target DNAs are covalently joined. We have carried out DNase I protection experiments on these protein-DNA complexes and found that only three MuA binding sites (L1, R1, and R2 of the six total) at the two Mu ends are stably bound by MuA to maintain the paired Mu end structure. The protection extends beyond the ends of the Mu sequence for different lengths (7-20 nucleotides) depending on the strand and the type of complex. After formation of the CDC, the other MuA binding sites (L2, L3, and R3) and internal activation sequence become dispensable for the subsequent strand-transfer reaction.
authors.affiliation: Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
authors.forename: M
authors.initials: M
authors.lastname: Mizuuchi
authors.forename: T A
authors.initials: TA
authors.lastname: Baker
authors.forename: K
authors.initials: K
authors.lastname: Mizuuchi
mesh_terms: D010583:Bacteriophage mu / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004251:DNA Transposable Elements; D003850:Deoxyribonuclease I; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D009713:Nucleotidyltransferases / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D015183:Restriction Mapping; D019895:Transposases
chemical_list: D004251:DNA Transposable Elements; D009838:Oligodeoxyribonucleotides; D009713:Nucleotidyltransferases; D019895:Transposases; D003850:Deoxyribonuclease I