The erythropoietin receptor gene: cloning and identification of multiple transcripts in an erythroid cell line OCIM1.
The hormone erythropoietin is necessary for the maintenance, generation, and maturation of erythroid cells. This kidney-derived glycoprotein binds to cell-surface receptors, mediating both the proliferation and differentiation of erythroid cells. We have characterized 16 human erythropoietin receptor cDNA clones from two cDNA libraries prepared from OCIM1 poly(A)-containing mRNA. Of these 16 isolates, many showed evidence of incorrect RNA splicing events; others contained small deletions yielding multiple transcripts that may play a role in their erythroleukemic state. The human erythropoietin receptor, as deduced from the sequence of these clones, encodes a 508-amino-acid molecule, including a 24-residue signal peptide, a 226-amino-acid external domain, a transmembrane spanning region of 22 amino acids, and a cytoplasmic domain of 236 amino acids. There is an 80% overall homology between the human and murine erythropoietin receptor sequences.
Ehrenman
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K
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington.
St John
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T
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002872:Chromosome Deletion; D004247:DNA / Q000737:chemistry*; D015723:Gene Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics*; D017467:Receptors, Erythropoietin
D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin; D004247:DNA