[Mechanisms of the joint action of cortisol and adenosine on peripheral blood lymphocytes in bronchial asthma].
The effect of adenosine (10(-6) M) on the cyclic AMP production in lymphocytes of peripheral blood of patients with bronchial asthma and healthy donors was studied. Cells response to adenosine-induced stimulation was significantly decreased for asthmatic patients as compared to normal subjects. Cortisol (3 x 10(-6) M) was shown to be able to normalize suppressed function of cells of the patients. Adenosine (10(-6) M) changed the distribution of hormone-receptor complexes in lymphocytes of the healthy donors to cause increment of the number of 3H cortisol-receptor complexes bound by nucleus. The treatment of lymphocytes of patients with adenosine didn't give any change in specific nuclear 3H cortisol binding. The obtained results may be used for a rational choice of hormonal therapy.
Sergeev
P V
PV
Dukhanin
A S
AS
Ogurtsov
S I
SI
Abazova
F I
FI
Omel'ianovskiĭ
V V
VV
Alieva
N G
NG
D000241:Adenosine / Q000008:administration & dosage / Q000494:pharmacology*; D001249:Asthma / Q000097:blood*; D002478:Cells, Cultured; D003470:Culture Media; D000242:Cyclic AMP / Q000096:biosynthesis / Q000097:blood*; D006801:Humans; D006854:Hydrocortisone / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D013268:Stimulation, Chemical
D003470:Culture Media; D000242:Cyclic AMP; D000241:Adenosine; D006854:Hydrocortisone
Mekhanizmy realizatsii sovmestnogo deĭstviia kortizola i adenozina na limfotsity perifericheskoĭ krovi pri bronkhial'noĭ astme.