Defective interfering particles of human parainfluenza virus type 3 are associated with persistent infection in cell culture.
CV-1 cell lines persistently infected with human parainfluenza virus type 3 (HPF3) contain one or more distinct subgenomic RNAs in addition to standard viral genomes. These RNAs are shown to be the genomes of defective-interfering (DI) particles of the virus; they are present in particles in the culture fluid, and they interfere with the growth of wild-type virus. Removal of the particles from the culture fluid by ultracentrifugation yields a supernatant fluid free from inhibitory activity, demonstrating that the anti-viral effect is not mediated by soluble factors. A role for the DI particles in persistence of HPF3 is considered.
Moscona
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Department of Pediatrics, Mount Sinai School of Medicine, New York, New York 10029-6574.
D002213:Capsid / Q000235:genetics; D002460:Cell Line; D003673:Defective Viruses / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D010224:Parainfluenza Virus 3, Human / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012367:RNA, Viral / Q000378:metabolism; D012995:Solubility; D014461:Ultracentrifugation; D014758:Viral Core Proteins / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
D012367:RNA, Viral; D014758:Viral Core Proteins