title: Isolation and characterization of a diuretic peptide from Acheta domesticus. Evidence for a family of insect diuretic peptides.
abstract: A diuretic peptide (Acheta-DP) has been isolated from extracts of whole heads of the house cricket, Acheta domesticus. The native peptide increases both cyclic AMP production and the rate of fluid secretion by isolated Malpighian tubules in vitro to an extent comparable with those responses obtained with supra-maximal amounts of crude extracts of corpora cardiaca. The primary structure of Acheta-DP was established as a 46-residue amidated peptide: TGAQSLSIVAPLDVLRQRLMNELNRRRMRELQGSRIQQNRQLLTSI-NH2. Acheta-DP has 41% sequence identity with a diuretic peptide isolated from Manduca sexta, providing direct evidence for the presence of a family of diuretic peptides in insects.
authors.affiliation: Department of Biology, Birkbeck College, University of London.
authors.forename: I
authors.initials: I
authors.lastname: Kay
authors.forename: G M
authors.initials: GM
authors.lastname: Coast
authors.forename: O
authors.initials: O
authors.lastname: Cusinato
authors.forename: C H
authors.initials: CH
authors.lastname: Wheeler
authors.forename: N F
authors.initials: NF
authors.lastname: Totty
authors.forename: G J
authors.initials: GJ
authors.lastname: Goldsworthy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000242:Cyclic AMP; D004232:Diuretics / Q000737:chemistry / Q000302:isolation & purification*; D006135:Gryllidae / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004232:Diuretics; D010455:Peptides; D000242:Cyclic AMP