Collagenase as an agent for dissolving the zona pellucida of hamster and mouse oocytes.
Crude preparations of collagenase, which have been used commonly for tissue dissociation, contain proteases that dissolve zonae pellucidae of hamster and mouse oocytes without reducing the ability of the oolemma to fuse with spermatozoa. This gentle proteolytic removal of zona is particularly useful for the study of sperm-oocyte fusion in mice, as trypsin, chymotrypsin and pronase damage the mouse oolemma.
Zuccotti
M
M
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822.
Urch
U A
UA
Yanagimachi
R
R
D000818:Animals; D006224:Cricetinae; D005260:Female; D008297:Male; D051379:Mice; D003012:Microbial Collagenase / Q000378:metabolism*; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D015044:Zona Pellucida / Q000378:metabolism*
D003012:Microbial Collagenase