Ion transport in cultured epithelia from human sweat glands: comparison of normal and cystic fibrosis tissues.
1. Cultured epithelia derived from whole human sweat glands, isolated secretory coils, isolated reabsorptive ducts and whole glands from cystic fibrosis (CF) subjects have been used to examine drug sensitivity by use of short circuit current recording. 2. Short circuit current increases were observed with lysylbradykinin, carbachol and histamine in epithelia of different origins. All responses were due to stimulation of electrogenic sodium absorption, evidenced by the inhibition of these responses by amiloride. The latter also abolished the basal current. The terpenes, thapsigargin and forskolin had no effect on transport. 3. The stimulation of a sodium current by agonists was dependent upon calcium, responses being inhibited by lanthanum ions and EGTA. Further A23187 induced a sodium current. 4. Pronounced oscillations in the sodium currents were a feature of the responses, implying synchronous, regulated cell activity. 5. Forskolin produced a ten fold increase in adenylate cyclase activity. All agonists listed in 2 except forskolin caused an increase in intracellular calcium [Ca]i, [Ca]i responses in CF cells were not different from those of normal cells, except with thapsigargin where the responses were smaller. 6. It is concluded that in culture, cells develop ductal characteristics, whether derived from normal or CF glands, coils or ducts. An increase in [Ca]i followed by activation of calcium-sensitive potassium channels and apical membrane hyperpolarization may be the major mechanism for increasing sodium influx.
Brayden
D J
DJ
Department of Pharmacology, University of Cambridge.
Pickles
R J
RJ
Cuthbert
A W
AW
D000262:Adenylyl Cyclases / Q000187:drug effects / Q000378:metabolism; D000584:Amiloride / Q000494:pharmacology; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism / Q000502:physiology; D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D003550:Cystic Fibrosis / Q000378:metabolism*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007609:Kallidin / Q000494:pharmacology; D007811:Lanthanum / Q000494:pharmacology; D015222:Sodium Channels / Q000187:drug effects; D013545:Sweat Glands / Q000187:drug effects / Q000378:metabolism*; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
D015222:Sodium Channels; D013729:Terpenes; D005576:Colforsin; D001464:Barium; D007609:Kallidin; D019284:Thapsigargin; D007811:Lanthanum; D000584:Amiloride; D002217:Carbachol; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D002118:Calcium