title: Development and functional characterization of monoclonal antibodies recognizing chicken lymphocytes with natural killer cell activity.
abstract: We have developed two monoclonal antibodies which detect cell surface antigens present on chicken lymphocytes mediating natural killer (NK) cell activity against the avian tumor cell target. The monoclonal antibodies, K-14 and K-108, stained 17 and 6% of splenic lymphocytes, and 11 and 14% of peripheral blood lymphocytes (PBL), respectively, and fewer than 5% of thymic and bursal lymphocytes. Neither of these monoclonal antibodies stained adherent macrophages or the MC29-virus transformed monocytic cell line. Both monoclonal antibodies significantly inhibited NK cell activity in a standard 4 h 51Cr-release cytotoxicity assay using the LSCC-RP9 tumor cell line as target cells at an effector to target ratio of 50:1. Pretreatment of splenocytes with either monoclonal antibody in the presence of rabbit complement (C) resulted in a significant reduction in NK cell activity. However, the monoclonal antibody K-1 which detects normal chicken macrophages did not interfere with NK cell activity. The monoclonal antibody K-108 significantly blocked Fc receptor-mediated rosette formation of sheep red blood cells coated with IgG antibodies (EA) by 56% while the monoclonal antibody K-14 did not show a significant blocking. These results indicate that the monoclonal antibodies K-108 and K-14 identify different epitopes present on the surface of chicken splenic lymphocytes which mediate spontaneous NK cytotoxicity.
authors.affiliation: Protozoan Diseases Laboratory, US Department of Agriculture, ARS, Beltsville, MD 20705.
authors.forename: K S
authors.initials: KS
authors.lastname: Chung
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007694:Killer Cells, Natural / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011205:Povidone; D051381:Rats; D011917:Rats, Inbred Lew; D012397:Rosette Formation; D012822:Silicon Dioxide
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone