Adsorption and preparation of human viruses using hydroxyapatite column.
The adsorption and chromatographic properties of hydroxyapatite sorbents for application to different viruses have been investigated. The strong adsorption of viruses was observed on macroporous hydroxyapatite with hydrophilic properties of the sorbent surface. The viruses were purified on this sorbent without loss of biological activity. The column can be used for virus vaccine production.
Tsuru
S
S
Department of Microbiology, National Defense Medical College, Saitama, Japan.
Shinomiya
N
N
Katsura
Y
Y
Uwabe
Y
Y
Noritake
M
M
Rokutanda
M
M
D000327:Adsorption; D005591:Chemical Fractionation / Q000379:methods*; D017886:Durapatite; D005069:Evaluation Studies as Topic; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D014776:Virus Cultivation; D014780:Viruses / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*
D006882:Hydroxyapatites; D017886:Durapatite