title: Protein phosphatase 2Bw and protein phosphatase Z are Saccharomyces cerevisiae enzymes.
abstract: cDNAs encoding three protein phosphatases, termed PP2Bw (Da Cruz e Silva, E.F. and Cohen, P.T.W. (1989) Biochim. Biophys. Acta 1009, 293-296), PPZ1 and PPZ2 that have been isolated from a Clontech 'rabbit brain' library are shown to be Saccharomyces cerevisiae clones. PPZ1 and PPZ2 are two novel yeast phosphatases showing 93% amino acid sequence identity to one another. PPZ1 shows approx. 60% sequence identity to S. cerevisiae or mammalian PP1 and approx. 40% identity to S. cerevisiae or mammalian PP2A. These and other observations suggest that the two isoforms of PPZ have functions distinct from those of PP1.
authors.affiliation: Department of Biochemistry, The University, Dundee, U.K.
authors.forename: E F
authors.initials: EF
authors.lastname: Da Cruz e Silva
authors.forename: V
authors.initials: V
authors.lastname: Hughes
authors.forename: P
authors.initials: P
authors.lastname: McDonald
authors.forename: M J
authors.initials: MJ
authors.lastname: Stark
authors.forename: P T
authors.initials: PT
authors.lastname: Cohen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004247:DNA; D010749:Phosphoprotein Phosphatases