Activity of the beta-retinoic acid receptor promoter in transgenic mice.
LacZ reporter gene constructs were used to analyze the murine retinoic acid receptor beta (mRAR beta) gene promoter in transgenic mice. LacZ expression in transgenic mouse embryos with 250 bp of promoter sequences closely parallels that of RAR beta between embryonic days 8.5 and 12.5. This indicates that the -1 to -250 promoter region contains most regulatory elements required for tissue specific expression. Additional elements in the -250 to -625 region are required for high expression levels after day 12.5. Elements in the -625 to -3100 region are necessary to reproduce the RAR beta expression in the meninges and the eye mesenchyme. The expression pattern of the transgene and the endogenous RAR beta, as revealed by in situ hybridization, suggests an important role of the RAR beta in the developing nervous system.
Reynolds
K
K
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892.
Mezey
E
E
Zimmer
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D004247:DNA; D004274:DNA, Recombinant; D005123:Eye / Q000196:embryology / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008578:Meninges / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic / Q000235:genetics; D018168:Receptors, Retinoic Acid; D001616:beta-Galactosidase / Q000235:genetics
D002352:Carrier Proteins; D004274:DNA, Recombinant; D018168:Receptors, Retinoic Acid; D004247:DNA; D001616:beta-Galactosidase