title: Simulation of the electrochemical behavior of multi-redox systems. Current potential studies on multiheme cytochromes.
abstract: The direct unmediated electrochemical response of the tetrahemic cytochrome c3 isolated from sulfate reducers Desulfovibrio baculatus (DSM 1743) and D. vulgaris (strain Hildenborough), was evaluated using different electrode systems [graphite (edge cut), gold, semiconductor (InO2) and mercury)] and different electrochemical methods (cyclic voltammetry and differential pulse voltammetry). A computer program was developed for the theoretical simulation of a complete cyclic voltammetry curve, based on the method proposed by Nicholson and Shain [Nicholson, R.S. & Shain, I. (1964) Anal. Chem. 36, 706-723], using the Gauss-Legendre method for calculation of the integral equations. The experimental data obtained for this multi-redox center protein was deconvoluted in to the four redox components using theoretically generated cyclic voltammetry curves and the four mid-point reduction potentials determined. The pH dependence of the four reduction potentials was evaluated using the deconvolution method described.
authors.affiliation: Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Moreno
authors.forename: A
authors.initials: A
authors.lastname: Campos
authors.forename: M
authors.initials: M
authors.lastname: Teixeira
authors.forename: J
authors.initials: J
authors.lastname: LeGall
authors.forename: M I
authors.initials: MI
authors.lastname: Montenegro
authors.forename: I
authors.initials: I
authors.lastname: Moura
authors.forename: C
authors.initials: C
authors.lastname: van Dijk
authors.forename: J G
authors.initials: JG
authors.lastname: Moura
mesh_terms: D002244:Carbon; D003574:Cytochrome c Group / Q000737:chemistry*; D003901:Desulfovibrio / Q000201:enzymology; D004563:Electrochemistry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008433:Mathematics; D008628:Mercury; D010084:Oxidation-Reduction; D012984:Software
chemical_list: D003574:Cytochrome c Group; D002244:Carbon; C017165:cytochrome c(3); D008628:Mercury