title: Increased sensitivity of a rotavirus serotyping enzyme-linked immunosorbent assay by the incorporation of CaCl2.
abstract: The sensitivity of a rotavirus serotyping enzyme-linked immunosorbent assay (ELISA) was improved by the addition of 0.5 mM CaCl2 to the washing buffer and reagent diluent. Twenty-nine of 63 (46%) previously untyped bovine and equine faecal rotavirus samples were serotyped in the modified assay. A differential response to Ca2+ ions was noted for different G-serotypes suggesting that serotyping assays performed without the inclusion of CaCl2 in the assay buffers may produce biased results.
authors.affiliation: Moredun Research Institute, Edinburgh, UK.
authors.forename: T A
authors.initials: TA
authors.lastname: Fitzgerald
authors.forename: G F
authors.initials: GF
authors.lastname: Browning
mesh_terms: D000818:Animals; D002021:Buffers; D002122:Calcium Chloride; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000382:microbiology; D012401:Rotavirus / Q000145:classification*; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods*
chemical_list: D002021:Buffers; D002122:Calcium Chloride