title: Superoxide chemical transformation of diolepoxide polyaromatic hydrocarbon DNA adducts. Determination of benzo[a]pyrene-r-7,t-8,9,c-10-tetrahydrotetrol by gas chromatography.
abstract: Benzo[a]pyrene-r-7,t-8,9,c-10-tetrahydrotetrol (100 pg, 342 fmol) was measured using the following sequence of steps: (1) chemical transformation with potassium superoxide to 2,3-pyrenedicarboxylic acid; (2) electrophore derivatization with pentafluorobenzyl bromide; (3) sample clean-up by high-performance liquid chromatography and (4) measurement by gas chromatography with electron-capture detection and by gas chromatography with electron-capture negative-ion mass spectrometry. The overall, absolute yields obtained by the two procedures were 69% and 60%, respectively. This work completes the first stage towards the establishment of a general method for detecting diolepoxide polyaromatic hydrocarbon DNA adducts by gas chromatography.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions, Boston, MA.
authors.forename: W N
authors.initials: WN
authors.lastname: Li
authors.forename: C
authors.initials: C
authors.lastname: Sotiriou-Leventis
authors.forename: S
authors.initials: S
authors.lastname: Abdel-Baky
authors.forename: D H
authors.initials: DH
authors.lastname: Fisher
authors.forename: R W
authors.initials: RW
authors.lastname: Giese
mesh_terms: D001564:Benzo(a)pyrene / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D008401:Gas Chromatography-Mass Spectrometry; D010084:Oxidation-Reduction; D013481:Superoxides / Q000737:chemistry*
chemical_list: D013481:Superoxides; D001564:Benzo(a)pyrene; C074998:benzo(a)pyrene-7,8,9,10-tetrol; D004247:DNA