Loss of pseudorabies virus thymidine kinase activity due to a single base mutation and amino acid substitution.
The nucleotide sequences of the coding region of the thymidine kinase (TK) gene of pseudorabies virus strain NIA3 and a TK- mutant (ATK5) were determined. The coding region of the TK gene consists of 320 codons capable of producing a polypeptide with an Mr of 34979. The mutant expressed an inactive TK polypeptide when translated in vitro; a unique base substitution was detected in the mutant TK modifying amino acid position 13, at which aspartic acid replaces glycine. This modification affects the nucleotide-binding site, thus explaining the expression of an inactive TK polypeptide.
Prieto
J
J
Departmento de Microbiologia, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
Martín Hernández
A M
AM
Tabarés
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E
D000595:Amino Acid Sequence; D000818:Animals; D001224:Aspartic Acid; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D005814:Genes, Viral; D005998:Glycine; D011558:Herpesvirus 1, Suid / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics*
D003062:Codon; D015678:Viral Structural Proteins; D001224:Aspartic Acid; D013937:Thymidine Kinase; D005998:Glycine