A microassay for protein glycation based on the periodate method.
The periodate assay for glycated protein has been adapted for use with a microplate reader. Up to 94 samples can be read in 40 s, and sensitivity has been improved so that only 2-40 nmol of protein-bound sugar are needed per well. Yields have been increased to over 90%, allowing in vitro glycation of human serum albumin to be assayed on 0.1-mg aliquots.
Ahmed
N
N
Oxford Research Unit, Open University, England.
Furth
A J
AJ
D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D010504:Periodic Acid; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000378:metabolism*
D006023:Glycoproteins; D012709:Serum Albumin; D010504:Periodic Acid; C009288:metaperiodate