title: Temporal changes in intracellular distribution of protein kinase C in Swiss 3T3 cells during mitogenic stimulation with insulin-like growth factor I and bombesin: translocation to the nucleus follows rapid changes in nuclear polyphosphoinositides.
abstract: Using a polyclonal antibody raised against a synthetic peptide of the catalytic region of protein kinase C, we have carried out a combined immunocytochemical and immunochemical analysis to follow the subcellular localisation of this enzyme in response to mitogenic stimulation with insulin-like growth factor I and bombesin. These investigations show a time dependent translocation of protein kinase C from the cytoplasm to the nucleus since 5 min stimulation reaching a maximal effect after 45 min. These results show clearly that mitogen induced translocation of protein kinase C to the nucleus follows temporally the earlier changes in nuclear polyphosphoinositide metabolism previously demonstrated.
authors.affiliation: Istituto di Anatomia Umana Normale, Bologna, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Martelli
authors.forename: L M
authors.initials: LM
authors.lastname: Neri
authors.forename: R S
authors.initials: RS
authors.lastname: Gilmour
authors.forename: P J
authors.initials: PJ
authors.lastname: Barker
authors.forename: N S
authors.initials: NS
authors.lastname: Huskisson
authors.forename: F A
authors.initials: FA
authors.lastname: Manzoli
authors.forename: L
authors.initials: L
authors.lastname: Cocco
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D002451:Cell Compartmentation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D013997:Time Factors
chemical_list: D009687:Nuclear Proteins; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D007334:Insulin-Like Growth Factor I; D011493:Protein Kinase C; D001839:Bombesin