Abnormal endogenous amino acid release in brain slices from vitamin B-6 restricted neonatal rats.
The basal and potassium-evoked efflux of glutamate, glycine, taurine, and gamma-aminobutyric acid (GABA) was measured in brain slices from vitamin B-6 restricted and sufficient 14-day-old rats. The results indicate a reduced level of basal glutamate, taurine, and GABA efflux in hippocampal slices and taurine and GABA in cortical slices from vitamin B-6 restricted animals. In the presence of depolarizing potassium concentrations, there was a reduced level of GABA efflux in hippocampal and cortical slices, and a marked reduction in the release of glutamate in cortical slices from B-6 restricted rats. The abnormalities in the secretion process of these neuroactive amino acids may be related to the neurological sequelae associated with neonatal vitamin B-6 restriction.
Guilarte
T R
TR
Department of Environmental Health Sciences, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001923:Brain Chemistry / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005260:Female; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D005998:Glycine / Q000378:metabolism; D066298:In Vitro Techniques; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D012640:Seizures / Q000503:physiopathology; D013654:Taurine / Q000378:metabolism; D026681:Vitamin B 6 Deficiency / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000596:Amino Acids; D005971:Glutamates; D009419:Nerve Tissue Proteins; D013654:Taurine; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D011188:Potassium; D005998:Glycine