title: Distinct calcium channels are generated by alternative splicing and are differentially expressed in the mammalian CNS.
abstract: A number of pharmacologically and electrophysiologically distinct voltage-dependent Ca2+ channels have been identified in mammalian neurons. Two rat brain Ca2+ channel alpha 1 subunits (rbC-I and rbC-II) have been isolated by molecular cloning and shown to be highly related (95%) to the cardiac dihydropyridine-sensitive Ca2+ channel. The rbC-II protein is distinct from rbC-I in that it contains a 3 amino acid insert in the putative cytoplasmic loop between domains II and III and a 28 amino acid substitution corresponding to the third transmembrane segment (S3) of the fourth domain. We show that rbC-I and rbC-II transcripts are generated by alternative splicing and that they are differentially expressed in the rat CNS.
authors.affiliation: Biotechnology Laboratory, Department of Neuroscience, University of British Columbia, Vancouver, Canada.
authors.forename: T P
authors.initials: TP
authors.lastname: Snutch
authors.forename: W J
authors.initials: WJ
authors.lastname: Tomlinson
authors.forename: J P
authors.initials: JP
authors.lastname: Leonard
authors.forename: M M
authors.initials: MM
authors.lastname: Gilbert
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism; D004274:DNA, Recombinant; D005796:Genes; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D012326:RNA Splicing; D051381:Rats; D014158:Transcription, Genetic; D014981:Xenopus
chemical_list: D015220:Calcium Channels; D004274:DNA, Recombinant