title: Studies on human porin. IV. The primary structures of "Porin 31HM" purified from human skeletal muscle membranes and of "Porin 31HL" derived from human B lymphocyte membranes are identical.
abstract: We report on the purification of "Porin 31HM" from the crude plasma membrane fraction of human skeletal muscle. Furthermore, all tryptic peptides of the molecule were purified and characterized by different methods. The alignment of the peptides with the complete primary structure of the human B lymphocyte plasma membrane-derived "Porin 31HL", published by us recently (Kayser, H. et al. (1989) this Journal 370, 1265-1278), proved both structures to be completely identical. Our data demonstrate that porin fractions from crude plasma membranes of different human cell types do not show any variation on the primary structure level.
authors.affiliation: Max-Planck-Institut für experimentelle Medizin, Abteilung Immunchemie, Göttingen.
authors.forename: L
authors.initials: L
authors.lastname: Jürgens
authors.forename: P
authors.initials: P
authors.lastname: Ilsemann
authors.forename: H D
authors.initials: HD
authors.lastname: Kratzin
authors.forename: D
authors.initials: D
authors.lastname: Hesse
authors.forename: K
authors.initials: K
authors.lastname: Eckart
authors.forename: F P
authors.initials: FP
authors.lastname: Thinnes
authors.forename: N
authors.initials: N
authors.lastname: Hilschmann
mesh_terms: D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000737:chemistry*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification; D008566:Membranes / Q000737:chemistry; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry* / Q000648:ultrastructure; D018272:Porins; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008565:Membrane Proteins; D018272:Porins