title: Thrombomodulin activity on human saphenous vein grafts prepared for coronary artery bypass.
abstract: Thrombomodulin, a membrane glycoprotein present on normal vascular endothelium, binds circulating thrombin and is important in protein C activation. These functions contribute to the nonthrombogenic nature of endothelium. Damage during harvest and ex vivo storage of vein grafts may result in dysfunction of this endothelial anticoagulant barrier and possibly contribute to early graft thrombosis. We studied the functional activity and antigenic expression of thrombomodulin on saphenous veins before (initial) and after (harvested) harvest and storage for coronary artery bypass grafting in 15 patients. Also, fresh saphenous vein was studied after mechanical endothelial stripping. After storage for 2.7 +/- 0.6 hours at room temperature in heparinized saline, thrombomodulin functional activity in harvested vein segments was 28% less than initial segments (p = 0.08). Endothelial stripping resulted in a 79% reduction in thrombomodulin activity compared with initial segments (p = 0.04). Immunohistochemical staining confirmed thrombomodulin antigen on vein grafts after harvest and storage, but not on segments stripped of endothelium. Thrombomodulin functional activity and antigenic expression on human saphenous vein grafts is not significantly changed by harvest and relatively short periods of storage at room temperature in heparinized saline.
authors.affiliation: Department of Surgery, John L. McClellan Veterans Administration Hospital, Little Rock, AR 72205.
authors.forename: J M
authors.initials: JM
authors.lastname: Cook
authors.forename: C D
authors.initials: CD
authors.lastname: Cook
authors.forename: R
authors.initials: R
authors.lastname: Marlar
authors.forename: M M
authors.initials: MM
authors.lastname: Solis
authors.forename: L
authors.initials: L
authors.lastname: Fink
authors.forename: J F
authors.initials: JF
authors.lastname: Eidt
mesh_terms: D000941:Antigens / Q000032:analysis / Q000378:metabolism; D001026:Coronary Artery Bypass / Q000379:methods*; D004730:Endothelium, Vascular / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D011486:Protein C / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000032:analysis / Q000378:metabolism*; D018179:Receptors, Thrombin; D012501:Saphenous Vein / Q000276:immunology / Q000378:metabolism* / Q000637:transplantation; D014021:Tissue Preservation
chemical_list: D000941:Antigens; D011486:Protein C; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin