Identification of an alpha 2-adrenoceptor in human coronary arteries by radioligand binding assay.
A single high affinity binding site for an alpha 2-adrenoceptor in human coronary arteries was identified by radioligand binding assay. Human coronary arteries were obtained at autopsy within 6 hours of death. A crude membrane solution was incubated with (3H)-rauwolscine at 25 degrees C for 30 min. The binding of (3H)-rauwolscine was rapidly saturable and reversible. Kd was 1.2 +/- 0.2 (SE) nM and Bmax 22 +/- 3 fmol/mg protein. This is the first study which has shown the presence of an alpha 2-adrenoceptor in human coronary arteries using a radioligand binding assay method.
Ishikawa
Y
Y
Second Department of Internal Medicine, Yokohama City University, School of Medicine, Japan.
Umemura
S
S
Uchino
K
K
Shindou
T
T
Yasuda
G
G
Minamisawa
K
K
Hayashi
S
S
Hirawa
N
N
Ishii
M
M
D001158:Arteries / Q000378:metabolism*; D001667:Binding, Competitive; D003331:Coronary Vessels / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D011869:Radioligand Assay; D011942:Receptors, Adrenergic, alpha / Q000737:chemistry*; D015016:Yohimbine / Q000378:metabolism
D011942:Receptors, Adrenergic, alpha; D015016:Yohimbine