The detection of melanocyte autoantibodies in the Smyth chicken model for vitiligo.
Smyth line (SL) chickens are phenotypically characterized by a posthatch depigmentation (vitiligo) of the feathers. The destruction of melanocytes in the feather follicle as well as in other tissues such as the choroid is genetically determined. Previous studies have shown that bursectomy or treatment with immunosuppressive agents decreases the incidence and severity of SL depigmentation (1). These observations implicate a role for the immune system, specifically the humoral component, in melanocyte destruction. In this study we show that there are circulating melanocyte-specific autoantibodies in the sera of depigmented SL chicks which are not present in sera from Light Brown Leghorn (LBL) control chicks. By immunoblots and by immunoprecipitation of radiolabeled melanocyte proteins, SL autoantibodies were shown to bind to multiple melanocyte proteins between 65 and 80 kDa. These proteins are not detected in SL fibroblasts. By immunoblotting, the incidence of autoantibodies for these 65- to 80-kDa proteins was determined to be 95% in depigmented SL chicks (n = 20), 0% in normally pigmented SL chicks (n = 8), and 5% in LBL chicks (n = 20). Melanocyte autoantibodies are detectable in the sera of affected chicks at or several weeks prior to the expression of depigmentation. This information, plus previously published data, implicate melanocyte autoantibodies in the depigmentary phenomenon of vitiligo observed in Smyth line chickens.
Austin
L M
LM
Department of Veterinary and Animal Science, University of Massachusetts, Amherst 01003.
Boissy
R E
RE
Jacobson
B S
BS
Smyth
J R
JR
D000367:Age Factors; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001323:Autoantibodies / Q000096:biosynthesis / Q000097:blood*; D002478:Cells, Cultured; D002645:Chickens; D004195:Disease Models, Animal; D015151:Immunoblotting; D008544:Melanocytes / Q000276:immunology*; D012880:Skin Pigmentation / Q000276:immunology; D014820:Vitiligo / Q000097:blood / Q000276:immunology*
D001323:Autoantibodies