Isolation of virus-like (VL30) elements from the Q10 and D regions of the major histocompatibility complex.
Previous studies from our laboratory have described two endogenous provirus-like sequences in a series of cosmids spanning the TL region of the major histocompatibility complex (MHC) of normal C57BL/10 mice. At least one of these viruses shares similarities with VL30 elements. To determine if additional VL30-like retroviral elements are integrated in the MHC, we constructed a cosmid library using DNA from a radiation leukemia virus (RadLV)-transformed cell line derived from C57BL/6 mice. The library was first screened using the H-2III (5') probe, which detects Class I genes of the H-2 complex. In the primary screening 163 H-2III positives were isolated. The H-2III-positive isolates were then hybridized with an AKR-derived virus probe, EcoB/S, which contains sequences from both the pol and the env genes of the virus. Nine virus-positive isolates were detected. Localization of these cosmid isolates containing viral sequences within the H-2 complex was done utilizing low-copy probes and confirmed using previously mapped cosmid isolates from other laboratories. We report here the isolation and characterization of VL30-like elements from the Qa and D regions of the MHC of several inbred mouse strains.
Choi
Y C
YC
Department of Pathology, New York University Medical Center, New York 10016.
Meruelo
D
D
D000406:AKR murine leukemia virus / Q000235:genetics; D000818:Animals; D003360:Cosmids; D015342:DNA Probes; D017988:Gammaretrovirus / Q000235:genetics / Q000302:isolation & purification*; D005786:Gene Expression Regulation; D015723:Gene Library; D005805:Genes, MHC Class I; D005814:Genes, Viral; D006183:H-2 Antigens / Q000235:genetics*; D064532:Histocompatibility Antigen H-2D; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics / Q000382:microbiology; D008810:Mice, Inbred C57BL / Q000235:genetics* / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*
D015342:DNA Probes; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D015395:Histocompatibility Antigens Class I; C032907:Q surface antigens