Immunohistochemical studies on complexes I, II, III, and IV of mitochondria in Parkinson's disease.
We examined the substantia nigra of 8 patients with Parkinson's disease immunohistochemically using antisera against complexes I, II, III, and IV of the mitochondrial electron transport system. In the patients with Parkinson's disease, a fair proportion of the nigral neurons showed reduced staining against the complex I antibody. The proportion of the neurons with reduced staining ranged from 12.7 to 74.1% of the melanized nigral neurons. Although neurons with reduced immunostaining for complex I were also observed in control subjects, the proportion among the nigral neurons was significantly smaller than in parkinsonian patients. Staining for complexes III and IV appeared normal. Staining of substantia nigra for complex II was decreased in 3 parkinsonian patients. These results are consistent with our findings that there is a deletion of gene coding for the four subunits in the mitochondrial DNA located in the striata of parkinsonian patients.
Hattori
N
N
Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan.
Tanaka
M
M
Ozawa
T
T
Mizuno
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004579:Electron Transport; D042963:Electron Transport Complex II; D014450:Electron Transport Complex III / Q000032:analysis; D003576:Electron Transport Complex IV / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000032:analysis; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000032:analysis; D010088:Oxidoreductases / Q000032:analysis*; D010300:Parkinson Disease / Q000201:enzymology*; D013378:Substantia Nigra / Q000201:enzymology*; D013385:Succinate Dehydrogenase / Q000032:analysis
D009097:Multienzyme Complexes; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D016660:NAD(P)H Dehydrogenase (Quinone); D003576:Electron Transport Complex IV; D014450:Electron Transport Complex III