First heterotransplantation of a human carcinoid tumor into nude mice.
The first successful heterotransplantation of a human carcinoid tumor into nude mice is reported. CSH, a voluminous hepatic metastasis of a primary bronchial carcinoid tumor (CSB) was resected and transplanted into three irradiated nude (Swiss-nu/nu) mice both by subcutaneous (SC) and intramuscular (IM) routes; the success rate was five of six. Heterotransplanted tumors took 4 to 5 months to appear in the mice and 1 month to attain a width of 0.5 cm. Both human and mouse tumors (named CSH-SC and CSH-IM) were studied by light and electron microscopy. They were Grimelius-positive, neuron-specific enolase-positive, and bombesin-negative by immunocytochemistry. Furthermore, CSH-SC cells presented characteristic (pear-shaped, rod-shaped, or tadpole-shaped) neurosecretory granules. Although CSB and CSH were slightly serotonin positive by immunocytochemistry, only a few serotonin-positive cells were found in CSH-SC and none in CSH-IM, suggesting partial loss of differentiation or an increase in serotonin catabolism during transplantation.
Ganem
Y J
YJ
Service de Biochimie et Neurobiologie, Hôpital Saint-Louis, Paris, France.
Launay
J M
JM
Debons-Guillemin
M C
MC
Lasneret
J
J
Roucayrol
A M
AM
Lesser
J
J
Peries
G
G
Dreux
C
C
D000368:Aged; D000818:Animals; D001984:Bronchial Neoplasms / Q000737:chemistry / Q000473:pathology*; D003528:Carcinoma, Adenoid Cystic / Q000737:chemistry / Q000473:pathology* / Q000556:secondary; D006801:Humans; D007150:Immunohistochemistry; D007273:Injections, Intramuscular; D007279:Injections, Subcutaneous; D008113:Liver Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000556:secondary; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008853:Microscopy; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation / Q000379:methods*; D012701:Serotonin / Q000032:analysis / Q000097:blood; D014183:Transplantation, Heterologous; D014916:Whole-Body Irradiation
D012701:Serotonin