The complete sequence of the group-specific antigen, VP7, of African horsesickness disease virus serotype 4 reveals a close relationship to bluetongue virus.
The complete sequence of the S7 RNA that codes for the major group-specific coat protein. VP7, of African horsesickness virus serotype 4 (AHSV-4) was determined from cDNA analyses and found to be 1179 nucleotides in length. One single open reading frame of 353 codons was observed defining a protein of Mr 38,107 with a net charge of -1.5 at neutral pH. Comparison of the AHSV-4 VP7 sequence with that of bluetongue virus serotype 10 revealed an overall similarity of 44%, with the amino- and carboxy-terminal regions exhibiting the greatest levels of homology. In addition, potential secondary structures of the terminal sequences of the S7 RNA segments of AHSV-4 and BTV serotypes 10, 13 and 17 are presented.
Roy
P
P
Department of Environmental Health Sciences, University of Alabama, Birmingham 35294.
Hirasawa
T
T
Fernandez
M
M
Blinov
V M
VM
Sanchez-Vixcain Rodrique
J M
JM
D000356:African Horse Sickness Virus / Q000145:classification / Q000235:genetics*; D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics*; D001820:Bluetongue virus / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D011487:Protein Conformation; D012367:RNA, Viral / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014758:Viral Core Proteins
D000956:Antigens, Viral; D012367:RNA, Viral; D014758:Viral Core Proteins; C069264:VP7 major core antigen, African horsesickness virus