title: Nasal mucosal hypersensitivity in guinea pigs intermittently exposed to cold.
abstract: To develop an animal model for experimental nasal hypersensitivity and hyperreactivity, guinea pigs were subjected to intermittent exposure to cold temperature (intermittent cold stress, SART stress) for 5 consecutive days. In SART-stressed guinea pigs, nasal mucosal hypersensitivity to histamine evoking sneeze response and nasal hypersecretion in response to methacholine were observed. The hypersensitivity remained for further 7 days after being released from SART stress. On the other hand, such nasal mucosal hypersensitivity was not caused by a continuous cold stress alone, suggesting that intermittent exposure to cold may be of importance for the appearance of nasal mucosal hypersensitivity. In passively sensitized SART-stressed guinea pigs, the quantity of nasal secretion induced by an allergen was significantly increased compared with that of a group of normal animals. The expression of muscarinic acetylcholine receptor (m-ACh.R) became higher in SART-stressed guinea pigs. Thus, hypersensitivity and hyperreactivity in this system were found to be associated with an increase in density of m-ACh.R. SART-stressed guinea pigs will serve as an animal model for hypersensitivity in nasal mucosa, which would be useful for the study of nasal allergy.
authors.affiliation: Institute of Bio-Active Science, Nippon Zoki Pharmaceutical Company, Hyogo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Namimatsu
authors.forename: K
authors.initials: K
authors.lastname: Go
authors.forename: T
authors.initials: T
authors.lastname: Hata
mesh_terms: D000818:Animals; D003080:Cold Temperature / Q000009:adverse effects*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006632:Histamine; D007106:Immune Sera / Q000008:administration & dosage; D008297:Male; D016210:Methacholine Chloride; D009297:Nasal Mucosa / Q000276:immunology* / Q000294:innervation / Q000378:metabolism; D009299:Nasal Provocation Tests; D010047:Ovalbumin / Q000276:immunology; D011942:Receptors, Adrenergic, alpha / Q000032:analysis; D011943:Receptors, Adrenergic, beta / Q000032:analysis; D011969:Receptors, Histamine H1 / Q000032:analysis; D011976:Receptors, Muscarinic / Q000032:analysis*; D012130:Respiratory Hypersensitivity / Q000209:etiology* / Q000276:immunology; D013312:Stress, Physiological / Q000276:immunology*
chemical_list: D007106:Immune Sera; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D011969:Receptors, Histamine H1; D011976:Receptors, Muscarinic; D016210:Methacholine Chloride; D006632:Histamine; D010047:Ovalbumin