title: Antibody response of kittens after vaccination followed by exposure to feline leukemia virus-infected cats.
abstract: Protein (western) blot analysis and virus-neutralization assay were used to evaluate the antibody response of specific-pathogen-free kittens to FeLV vaccination and followed by natural exposure. Several kittens had barely detectable reactions to specific FeLV antigens prior to vaccination or exposure. Correlation was not found between protection against persistent viremia and antibody response after vaccination as measured by western blot analysis or virus neutralization assay. A statistically significant (P less than 0.01) difference in the antibody response against p27 antigen after natural exposure to FeLV was observed between persistently viremic kittens and transiently viremic or aviremic kittens. Measurable (P less than 0.05) virus neutralizing antibody titer after FeLV exposure was found only in a small number of kittens that were protected against persistent viremia. Lack of association between humoral response and vaccination-induced protection against persistent FeLV infection suggests an important role for cell-mediated immunity in such protection.
authors.affiliation: Department of Urban Practice, College of Veterinary Medicine, University of Tennessee, Knoxville 37901.
authors.forename: D M
authors.initials: DM
authors.lastname: Hawks
authors.forename: A M
authors.initials: AM
authors.lastname: Legendre
authors.forename: B W
authors.initials: BW
authors.lastname: Rohrbach
authors.forename: R
authors.initials: R
authors.lastname: Sebring
authors.forename: L
authors.initials: L
authors.lastname: Chavez
authors.forename: H J
authors.initials: HJ
authors.lastname: Chu
authors.forename: W M
authors.initials: WM
authors.lastname: Acree
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D015153:Blotting, Western; D001853:Bone Marrow / Q000382:microbiology; D002415:Cats; D005256:Leukemia Virus, Feline / Q000276:immunology* / Q000302:isolation & purification; D016582:Leukemia, Feline / Q000517:prevention & control*; D009500:Neutralization Tests; D011897:Random Allocation; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000662:veterinary*; D014765:Viral Vaccines / Q000276:immunology*; D014766:Viremia / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral; D015682:Retroviridae Proteins, Oncogenic; D014765:Viral Vaccines; C045569:feline leukemia virus vaccine