Thyrotrophin-releasing hormone-induced growth hormone (GH) secretion in anaesthetized chickens: inhibition by GH-releasing factor at central sites.
Intracerebroventricular (i.c.v.) administration of GH-releasing factor (GRF) (at 1 or 10 micrograms) to anaesthetized immature (6- to 8-weeks-old) or adult (greater than 24-weeks-old) domestic fowl had no effect on basal GH concentrations in peripheral plasma, but suppressed (after 20 min) the acute GH response to exogenous (i.v.) thyrotrophin-releasing hormone (TRH) (1 micrograms/kg). The i.c.v. injection of GRF also reduced the content of somatostatin (SRIF) and dopamine (DA) in the hypothalamus, while increasing the concentration of the DA metabolite 3,4-dihydroxyphenyl acetic acid (DOPAC) and the DOPAC/DA ratio. The release of SRIF from hypothalamic tissue was stimulated in vitro by 100 nmol GRF/l. The inhibitory effect of i.c.v. GRF on TRH-induced GH secretion was blocked when it was simultaneously injected i.c.v. with SRIF antiserum. These results demonstrate central effects of GRF on avian hypothalamic function and suggest an inhibitory role for this peptide in GH regulation, possibly mediated through increased SRIF secretion.
Ahene
C A
CA
Department of Physiology, University of Alberta, Edmonton, Canada.
Lea
R W
RW
Harvey
S
S
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000768:Anesthesia, General; D000818:Animals; D002645:Chickens; D003864:Depression, Chemical; D004298:Dopamine / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism*; D013007:Growth Hormone-Releasing Hormone / Q000008:administration & dosage* / Q000494:pharmacology; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007276:Injections, Intraventricular; D008297:Male; D009924:Organ Culture Techniques; D013004:Somatostatin / Q000378:metabolism / Q000494:pharmacology; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology*
D015102:3,4-Dihydroxyphenylacetic Acid; D013004:Somatostatin; D013973:Thyrotropin-Releasing Hormone; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone; D004298:Dopamine