The gene encoding cytochrome c oxidase subunit II from Rhodobacter sphaeroides; comparison of the deduced amino acid sequence with sequences of corresponding peptides from other species.
The gene (coxII) encoding subunit II of Rhodobacter sphaeroides cytochrome c oxidase (cytochrome aa3) has been isolated by screening a genomic DNA library in phage lambda with a probe derived from coxII of Paracoccus denitrificans. A 2-kb fragment containing coxII DNA was subcloned into the phage M13mp18 and the sequence determined. The 2-kb insert contains the entire coding region for coxII gene, including the ATG start codon and a TGA stop codon. The deduced amino acid (aa) sequence of subunit II of R. sphaeroides shows regions of substantial homology to the corresponding subunit of the bovine mitochondrial oxidase (63% overall) and P. denitrificans oxidase (68% overall). The postulated redox-active copper ion (CuA) binding site involving two Cys and two His residues (as well as an alternative Met residue) is conserved among these species, along with four invariant acidic aa residues (two Asp and two Glu) that may be involved in interactions with cytochrome c, and a region of aromatic residues (Tyr-Gln-Trp-Tyr-Trp-Gly-Tyr-Glu-Tyr) which is postulated to play a role in electron transfer. Hydropathy profile analysis suggests that while the bovine COXII secondary structure contains two transmembrane helices, the R. sphaeroides subunit II has a third such helix that may function as part of a signal sequence, as suggested for P. denitrificans.
Cao
J
J
Department of Biochemistry, Michigan State University, East Lansing 48824.
Shapleigh
J
J
Gennis
R
R
Revzin
A
A
Ferguson-Miller
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D004274:DNA, Recombinant; D003576:Electron Transport Complex IV / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012242:Rhodobacter sphaeroides / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004274:DNA, Recombinant; D003576:Electron Transport Complex IV