Characterization of a full-length cDNA which codes for the human spermidine/spermine N1-acetyltransferase.
Spermidine/spermine N1-acetyltransferase is the rate-limiting enzyme in the catabolism of cellular polyamines. Using a combination of cDNA library screening and anchored PCR methodologies, a full length cDNA designated AP3/F7 corresponding to the human SSAT was cloned using RNA from the human large cell undifferentiated lung carcinoma line NCI H157. The resulting cDNA clone is 1,060 base pairs with a 513 base open reading frame coding for a 171 amino acid protein, with a predicted subunit molecular weight of 20,023. The 5' non-coding region of AP3/F7 is 165 bases and the 3' untranslated region is 382 bases with a polyadenylation site 20 bases 5' to the poly(A) tail. This full length cDNA should be an aid in the study of the regulation of spermidine/spermine N1-acetyltransferase expression and the significance of the acetyltransferase in polyamine metabolism.
Xiao
L
L
Oncology Center Laboratories, Johns Hopkins University School of Medicine, Baltimore, MD 21231.
Celano
P
P
Mank
A R
AR
Pegg
A E
AE
Casero
R A
RA
D000123:Acetyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002289:Carcinoma, Non-Small-Cell Lung; D002460:Cell Line; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008175:Lung Neoplasms; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000379:methods; D014176:Protein Biosynthesis; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D014158:Transcription, Genetic; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes; D012334:RNA, Neoplasm; D000099228:Diamine N-Acetyltransferase