Isolation of Gln- mutants through transposon, Tn917, mutagenesis in Bacillus brevis.
Transposon, Tn917, carried on pTV1 plasmid has been used successfully to mutagenise Bacillus brevis. The transposon showed preference for insertion at an "aro" site. A second insertional event after elimination of the preferred site with ethidium bromide/acridine orange treatment has permitted isolation of Gln- mutants in B. brevis.
Mishra
H S
HS
Department of Biochemistry, G.B. Pant University of Agriculture & Technology, Pantnagar.
Mishra
D P
DP
Garg
G K
GK
D001407:Bacillus / Q000235:genetics*; D004251:DNA Transposable Elements; D005973:Glutamine / Q000235:genetics; D016254:Mutagenesis, Insertional
D004251:DNA Transposable Elements; D005973:Glutamine