Isotachophoresis of proteins in uncoated open-tubular fused-silica capillaries with a simple approach for column conditioning.
The isotachophoretic determination of proteins in uncoated open-tubular fused-silica capillaries of 50 and 75 microns I.C. with on-column multi-wavelength detection is reported. Small amounts of hydroxypropylmethylcellulose added to the leader provide an efficient method of dynamic column conditioning which allows the high-resolution isotachophoretic determination of most proteins to be performed in the presence of an electro-osmotic flow. Different approaches for cationic and anionic analyses are discussed and illustrated with selected examples.
Gebauer
P
P
Department of Clinical Pharmacology, University of Berne, Switzerland.
Thormann
W
W
D000818:Animals; D000838:Anions; D002256:Carbonic Anhydrases / Q000032:analysis; D002412:Cations; D002417:Cattle; D002645:Chickens; D004586:Electrophoresis / Q000379:methods*; D010047:Ovalbumin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012260:Ribonucleases / Q000032:analysis; D012822:Silicon Dioxide; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D000838:Anions; D002412:Cations; D011506:Proteins; D005680:gamma-Aminobutyric Acid; D012822:Silicon Dioxide; D010047:Ovalbumin; D012260:Ribonucleases; D002256:Carbonic Anhydrases