Bimane fluorogenic substrates for microdetermination of angiotensin converting enzyme level in serum.
The fluorescence of 9,10-dioxa-syn-3,4,6,7-tetramethylbimane (bimane) was found to be quenched in the presence of nitrated aromatic amino acid. Bimane peptides containing nitrated amino acid (1a, b) were shown to be useful fluorogenic substrates for the assay of angiotensin I converting enzyme (ACE) from rabbit lung, similar to bimane substrate containing tryptophan (3) previously reported. Among these bimane substrates, substrate 3 was shown to be a potent fluorogenic substrate for microdetermination of the ACE level in human serum.
Sato
E
E
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Hattori
H
H
Nishikawa
S
S
Kanaoka
Y
Y
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D001643:Bridged Bicyclo Compounds / Q000737:chemistry*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005456:Fluorescent Dyes; D006801:Humans; D007703:Peptidyl-Dipeptidase A / Q000097:blood*
D000806:Angiotensin-Converting Enzyme Inhibitors; D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005456:Fluorescent Dyes; C021149:bimanes; D007703:Peptidyl-Dipeptidase A