[Use of the Dot-Blot technique in the detection of human papillomavirus(HPV) deoxyribonucleic acid (DNA) in malignant tumors of the oropharynx].
A total of 23 malignant oropharyngeal tumours (palate, tongue, tonsils, pharynx) and two lymph node metastases were analysed for presence of papilloma virus DNA. Thirteen (54.5%) of 22 squamous cell cancers of different koilocytosis and grading were found to contain HPV 16/18 DNA. Only 16.6% (4 cases) were positive for HPV 6/11 DNA. There was no detectable HPV-DNA in the lymph node metastases (Table 1). The control biopsies (19) were negative after hybridisation.
Arndt
O
O
Hals-, Nasen- und Ohrenklinik Otto Körner, Universität Rostock.
Bauer
I
I
Brock
J
J
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D004273:DNA, Neoplasm / Q000276:immunology; D004279:DNA, Viral / Q000276:immunology*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008875:Middle Aged; D009959:Oropharyngeal Neoplasms / Q000276:immunology*; D027383:Papillomaviridae / Q000276:immunology*
D004273:DNA, Neoplasm; D004279:DNA, Viral
Nachweis humaner Papillomvirus-(HPV-)Desoxyribonukleinsäure (DNA) in malignen Tumoren des Oropharynx mittels Dot-Blot-Technik.