title: Nucleotide sequence analysis of Aleutian mink disease parvovirus shows that multiple virus types are present in infected mink.
abstract: Different isolates of Aleutian mink disease parvovirus (ADV) were cloned and nucleotide sequenced. Analysis of individual clones from two in vivo-derived isolates of high virulence indicated that more than one type of ADV DNA were present in each of these isolates. Analysis of several clones from two preparations of a cell culture-adapted isolate of low virulence showed the presence of only one type of ADV DNA. We also describe the nucleotide sequence from map units 44 to 88 of a new type of ADV DNA. The new type of ADV DNA is compared with the previously published ADV sequences, to which it shows 95% homology. These findings indicate that ADV, a single-stranded DNA virus, has a considerable degree of variability and that several virus types can be present simultaneously in an infected animal.
authors.affiliation: Department of Veterinary Microbiology, Royal Veterinary and Agricultural University of Copenhagen, Frederiksberg C, Denmark.
authors.forename: E
authors.initials: E
authors.lastname: Gottschalck
authors.forename: S
authors.initials: S
authors.lastname: Alexandersen
authors.forename: A
authors.initials: A
authors.lastname: Cohn
authors.forename: L A
authors.initials: LA
authors.lastname: Poulsen
authors.forename: M E
authors.initials: ME
authors.lastname: Bloom
authors.forename: B
authors.initials: B
authors.lastname: Aasted
mesh_terms: D000453:Aleutian Mink Disease / Q000382:microbiology*; D000454:Aleutian Mink Disease Virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry*; D008907:Mink; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence
chemical_list: D004279:DNA, Viral