Analysis of cellular DNA content using flow cytometry in skin squamous cell carcinoma and its relationship to clinical behaviors.
Our report includes a technique using the flow cytometer to analyze the cellular DNA content of paraffin-embedded histological material from tumors. This technique allows for the retrospective study of tumors from patients whose clinical outcome is already known. We have applied this process to study skin squamous cell carcinomas (SCCs). We reviewed and studied 34 patients positive for SCCs, With or without lymph node or distant metastasis. All received radical treatment from December 1982 to December 1988. The DNA distribution pattern of SCCs in relation to the clinical stage and the histological grade was evaluated. Our findings indicated that patients with diploid tumors had a better prognosis than those with aneuploid tumors. The DNA distribution pattern correlates well with the clinical stage and is a better determinant than histological grading or clinical behaviors.
Wan
H L
HL
Department of Surgery, Veterans General Hospital-Taichung, Taiwan, R.O.C.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012878:Skin Neoplasms / Q000737:chemistry* / Q000473:pathology
D004273:DNA, Neoplasm; D004247:DNA