title: Peroxidase activity loss after filtration and centrifugation of whole saliva. Influence of citrate.
abstract: Filtration or centrifugation are two methods frequently used to clarify human whole saliva. This study compares both the drop in peroxidase activity following filtration (49.3% +/- 16.4%; N = 12) or centrifugation (33.6% +/- 15.9%; N = 6) and the drop in total protein concentration after filtration (33.9% +/- 13.4%) and centrifugation (23.6% +/- 8.7%). After filtration, the loss was not only due to retention of the large particle-bound fraction but also to adsorption on the filter membranes. This adsorption could be inhibited by a previous dilution of saliva in a citrate buffer, which also gave rise to active subunits with a molecular weight less than or equal to 100 kDa.
authors.affiliation: Laboratoire de Stomatologie, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Vanden Abbeele
authors.forename: P
authors.initials: P
authors.lastname: Courtois
authors.forename: M
authors.initials: M
authors.lastname: Pourtois
mesh_terms: D000327:Adsorption; D002021:Buffers; D002498:Centrifugation; D002951:Citrates / Q000737:chemistry*; D019343:Citric Acid; D004591:Electrophoresis, Polyacrylamide Gel; D005374:Filtration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010544:Peroxidases / Q000032:analysis* / Q000737:chemistry; D012463:Saliva / Q000201:enzymology*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D014462:Ultrafiltration; D014867:Water / Q000737:chemistry
chemical_list: D002021:Buffers; D002951:Citrates; D012471:Salivary Proteins and Peptides; D014867:Water; D019343:Citric Acid; D010544:Peroxidases