title: Partial sequences of high-molecular-weight cytochrome c isolated from Desulfovibrio vulgaris Miyazaki.
abstract: A polyhemic high-molecular-weight cytochrome c (hmc) of Mr 67,000 was purified from Desulfovibrio vulgaris Miyazaki. This cytochrome is composed of a single peptide chain. Unlike other c-type cytochromes, hmc deteriorates slowly in atmospheric oxygen. Two peptide fragments of Mr 33,100 (L peptide) and Mr 19,100 (S peptide) were isolated and their N-terminal sequences determined to be PLPGATGEQRADLVEIGVMAKFTNLELPKV (L peptide) and GTLPAVAIPEFVTIGVLK (S peptide). The established sequence of the L peptide is homologous to the N-terminal sequence of cytochrome c3 (Mr 26,000) from D. vulgaris Hildenborough, in spite of discrepancies observed in the molecular size and subunit structure of these cytochromes.
authors.affiliation: Department of Chemistry, Shizuoka University, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Tasaka
authors.forename: M
authors.initials: M
authors.lastname: Ogata
authors.forename: T
authors.initials: T
authors.lastname: Yagi
authors.forename: A
authors.initials: A
authors.lastname: Tsugita
mesh_terms: D000595:Amino Acid Sequence; D003574:Cytochrome c Group / Q000737:chemistry* / Q000302:isolation & purification; D016969:Desulfovibrio vulgaris / Q000201:enzymology*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D012984:Software; D013053:Spectrophotometry; D013057:Spectrum Analysis
chemical_list: D003574:Cytochrome c Group