title: Experimental and theoretical studies of DNA separations by capillary electrophoresis in entangled polymer solutions.
abstract: Studies of electrophoretic separations of DNA restriction fragments by capillary electrophoresis in solutions of (hydroxyethyl) cellulose (HEC) were performed. Rheological studies were used to confirm that the entanglement threshold (phi*) for the HEC solutions used is approximately 0.004 g/mL, in good agreement with theoretical predictions. A mesh size an order of magnitude smaller than that found in agarose gels (on a per weight basis) was calculated using polymer-entanglement theory and was confirmed by electrophoretic measurements. Electrophoretic migration was shown to follow the Ogston regime under most conditions. An approach for obtaining smaller mesh sizes is presented.
authors.affiliation: Department of Chemical Engineering, University of California, Berkeley 94720.
authors.forename: P D
authors.initials: PD
authors.lastname: Grossman
authors.forename: D S
authors.initials: DS
authors.lastname: Soane
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods*; D008956:Models, Chemical; D012996:Solutions / Q000737:chemistry; D014783:Viscosity
chemical_list: D012996:Solutions; D002482:Cellulose; C002283:hydroxyethylcellulose; D004247:DNA; D004262:DNA Restriction Enzymes