Japanese encephalitis virus fusion protein with protein A expressed in Escherichia coli confers protective immunity in mice.
A complementary DNA (cDNA) that codes C-terminal, one-third of envelope glycoprotein (E) and N-terminal 65 amino acids of NS1 protein of Japanese encephalitis (JE) virus was inserted into Escherichia coli expression vector pRIT2T. The inserted gene was expressed as a fusion protein with protein A, and the expressed protein was intraperitoneally injected into mice. The immunized mice produced anti-JE antibodies measured by the hemagglutination-inhibition and neutralization tests as well as ELISA and were protected from the lethal challenge of JE virus by intraperitoneal inoculation.
Srivastava
A K
AK
Department of Virology, Nagasaki University, Japan.
Morita
K
K
Matsuo
S
S
Tanaka
M
M
Igarashi
A
A
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology* / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006387:Hemagglutination, Viral; D007109:Immunity; D051379:Mice; D009500:Neutralization Tests; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000276:immunology; D013205:Staphylococcal Protein A / Q000235:genetics*; D014162:Transfection; D014760:Viral Fusion Proteins / Q000235:genetics*
D000914:Antibodies, Viral; D004279:DNA, Viral; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; D014760:Viral Fusion Proteins