title: Etiology of rabbit haemorrhagic disease spontaneously occurring in Korea.
abstract: Causative agent of rabbit haemorrhagic disease (RHD) was purified by CsCl density gradient centrifugation from the liver homogenate of rabbits infected with RHD virus which originated from Korea. The viral particles were 35-40 nm in diameter, and had hollow depressions on their surface. Protein A-gold immunoelectron microscopy clearly showed that the convalescent antisera of diseased rabbits reacted specifically with the virus particles. SDS-PAGE and Western blot analyses demonstrated that the structural protein of the virus was composed of a single major polypeptide of 63 kD. These findings indicate that the causative agent of RHD, tentatively named as picornavirus in Korea, belongs to calicivirus.
authors.affiliation: Department of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Japan.
authors.forename: J H
authors.initials: JH
authors.lastname: Park
authors.forename: H
authors.initials: H
authors.lastname: Kida
authors.forename: K
authors.initials: K
authors.lastname: Ueda
authors.forename: K
authors.initials: K
authors.lastname: Ochiai
authors.forename: M
authors.initials: M
authors.lastname: Goryo
authors.forename: C
authors.initials: C
authors.lastname: Itakura
mesh_terms: D000818:Animals; D002139:Caliciviridae / Q000145:classification* / Q000648:ultrastructure; D006470:Hemorrhage / Q000382:microbiology / Q000662:veterinary*; D008854:Microscopy, Electron; D010850:Picornaviridae Infections / Q000382:microbiology / Q000662:veterinary*; D011817:Rabbits; D015678:Viral Structural Proteins / Q000032:analysis*
chemical_list: D015678:Viral Structural Proteins