title: Molecular cloning, sequence analysis and coding assignment of the major inner capsid protein gene of human group C rotavirus.
abstract: The VP6 gene of human group C rotavirus was cloned and sequenced. Hybridization to the human group C and the porcine group C/Cowden dsRNA genomes assigned this coding sequence to segment 5. The complete human VP6 sequence contained an open reading frame of 1185 nucleotides (395 amino acids; deduced Mr 44,669 Da). The protein sequence demonstrated low homology with the group A VP6 sequences (41.7 to 42.7%) and high homology (88.9%) with the porcine group C VP6 sequence. However, the protein sequence alignments revealed a region of 10 amino acids that were significantly different between the human and the porcine group C viruses.
authors.affiliation: Department of Microbiology, University Medical School, Southampton General Hospital, United Kingdom.
authors.forename: S J
authors.initials: SJ
authors.lastname: Cooke
authors.forename: P R
authors.initials: PR
authors.lastname: Lambden
authors.forename: E O
authors.initials: EO
authors.lastname: Caul
authors.forename: I N
authors.initials: IN
authors.lastname: Clarke
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral; D001483:Base Sequence; D015152:Blotting, Northern; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D009841:Oligonucleotides; D012367:RNA, Viral; C061332:VP6 protein, Rotavirus; D015678:Viral Structural Proteins; D004247:DNA