Tn1725 transposon mutagenesis of 9-18 delta 7, an EcoRI deletion derivative of Salmonella dublin lane plasmid pSDL2.
A 37.5-kb derivative of the Salmonella dublin virulence plasmid pSDL2 was subjected to mutagenesis with the transposon Tn1725. Fifty-two insertions were mapped, and the mutants were tested for their ability to restore virulence to a plasmid-free strain of S. dublin. Twenty-nine of these inserts could not restore full virulence and thus define nine regions of the plasmid essential for virulence. Deletion of a 4.5-kb region by Bal31 nuclease resulted in a 33-kb derivative that maintained full virulence.
Daifuku
R
R
Department of Medicine, University of California, Los Angeles 90024-1688.
Chikami
G K
GK
D000818:Animals; D004251:DNA Transposable Elements; D051379:Mice; D008807:Mice, Inbred BALB C; D016296:Mutagenesis; D010957:Plasmids; D012475:Salmonella / Q000235:genetics* / Q000472:pathogenicity; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000494:pharmacology; D014774:Virulence
D004251:DNA Transposable Elements; C020098:DNA modification methylase EcoRI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)