Identification and DNA sequence of the large subunit of the capping enzyme from Shope fibroma virus.
A 3.6-kb region of the Shope fibroma virus (SFV) BamHI D fragment located in the central region of the viral genome was sequenced. Three open reading frames (ORFs) were identified, D3R, D4L, and D5R. Each of these ORFs have a counterpart organized identically within the HindIII fragment D of the vaccinia virus genome (D1R, D2L, and D3R). Homology scores and assays of viral cores indicate that SFV D3R encodes the large subunit of the SFV mRNA capping enzyme.
Upton
C
C
Department of Biochemistry, University of Alberta, Edmonton, Canada.
Stuart
D
D
McFadden
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D012776:Fibroma Virus, Rabbit / Q000201:enzymology / Q000235:genetics*; D005814:Genes, Viral; D008780:Methyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D009713:Nucleotidyltransferases / Q000235:genetics*; D016366:Open Reading Frames; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D012315:RNA Caps; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins
D004279:DNA, Viral; D009097:Multienzyme Complexes; D012315:RNA Caps; D014764:Viral Proteins; D008780:Methyltransferases; D009713:Nucleotidyltransferases; D010744:Phosphoric Monoester Hydrolases; C027731:capping enzyme, vaccinia virus