[A new enzyme immunoassay for the detection of antibodies against the bovine viral diarrhea virus].
An enzyme-linked immunosorbent assay (ELISA), using the nonstructural protein p125/80 of the bovine viral diarrhoea (BVD) virus as antigen, was used for screening BVD-specific antibodies in 468 bovine sera. The results were compared with those obtained in a standard neutralization test (NT). Both tests reacted positive with 457 sera. The ELISA gave positive results with eight sera which were negative in the NT whereas one serum was positive in the NT and negative in the ELISA. Two sera could not be tested in the NT because they were toxic for the cultured cells. In addition the sera were screened in an ELISA using the equivalent heterologous nonstructural protein of hog cholera virus as antigen. The results obtained in both ELISAs showed a correlation of 94%.
Moennig
V
V
Institut für Virologie, Tierärztlichen Hochschule, Hannover.
Leder
L
L
Greiser-Wilke
I
I
Frey
H R
HR
Liess
B
B
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D002417:Cattle; D006692:Classical Swine Fever Virus / Q000276:immunology; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D009500:Neutralization Tests
D000914:Antibodies, Viral; D000956:Antigens, Viral
Ein neuer Enzymimmuntest zum Nachweis von Antikörpern gegen das Virus der bovinen Virusdiarrhö.