title: An Escherichia coli rpoB mutation that inhibits transcription of catabolite-sensitive operons.
abstract: The Escherichia coli rpoB636 mutant is defective in the transcription of lac and other catabolite-sensitive operons. The lac promoter variant, UV5, which is independent of cyclic AMP and the cyclic AMP receptor protein, CRP, was also defective in rpoB636 mutants. The activity of the lac UV5 promoter was restored to wild-type levels by deletion of cya (adenylate cyclase) or crp. Cyclic AMP and CRP apparently act as inhibitors of the rpoB636 RNA polymerase.
authors.affiliation: Institute of Cancer Research, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
authors.forename: P
authors.initials: P
authors.lastname: Rockwell
authors.forename: M E
authors.initials: ME
authors.lastname: Gottesman
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D000242:Cyclic AMP / Q000502:physiology; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D011401:Promoter Regions, Genetic; D011953:Receptors, Cyclic AMP / Q000502:physiology*; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D011953:Receptors, Cyclic AMP; D000242:Cyclic AMP; D012321:DNA-Directed RNA Polymerases