title: Cloning of a cDNA encoding an Artemia franciscana Na/K ATPase alpha-subunit.
abstract: Clones of cDNA that code for an isoform of the Artemia franciscana Na/K ATPase alpha subunit (NaKA alpha) have been isolated. The sequence of the longest of these clones (pArATNa136) is 3595 nucleotides; it codes for a 1004-amino acid protein whose sequence is identical to that of two previously sequenced Artemia NaKA alpha peptides. The encoded protein is over 73% identical to Drosophila melanogaster and vertebrate NaKA alpha s, and 73.8% identical to another Artemia NaKA alpha isoform previously described (named alpha 2850 in this article). The two Artemia cDNA clones code for mRNAs of different size; the clone pArATNa136 codes for a 4.5-kb mRNA while the alpha 2850 clone codes for a 3.6-kb mRNA. The degree of homology and the different size of the mRNAs encoded by both cDNAs suggest that they code for two different isoforms of the protein.
authors.affiliation: Instituto de Investigaciones Biomédicas del C.S.I.C., Madrid, Spain.
authors.forename: M T
authors.initials: MT
authors.lastname: Macías
authors.forename: I
authors.initials: I
authors.lastname: Palmero
authors.forename: L
authors.initials: L
authors.lastname: Sastre
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D015183:Restriction Mapping; D016415:Sequence Alignment; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D000254:Sodium-Potassium-Exchanging ATPase