Primary structure of bovine endothelin ETB receptor and identification of signal peptidase and metal proteinase cleavage sites.
A cDNA clone corresponding to the entire coding region of the bovine ETB endothelin receptor mRNA was isolated from a lung cDNA library and sequenced. The cDNA encodes 441 amino acids: 26 constituting an NH2-terminal signal peptide and 415 constituting the mature receptor. The signal peptidase cleavage site was determined by direct amino acid sequencing of purified receptor. A comparison of the predicted amino acid sequence with the available bovine ETA and rat ETB endothelin receptor sequences revealed 63 and 85% homology, respectively. Endothelin receptors of various species are known to be very sensitive to a certain metal proteinase(s) and have been shown to be converted to a lower Mr form in the absence of EDTA. The metal proteinase cleavage site was also determined by direct protein sequencing of the proteolysis product. The amino acid sequence (Ala-Gly-X-Pro-Pro-Arg) surrounding the cleavage site (between Ala-79 and Gly-80) is conserved among the ETB endothelin receptors, explaining the above mentioned proteolytic conversion from the higher to lower Mr forms observed in various species.
Saito
Y
Y
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Mizuno
T
T
Itakura
M
M
Suzuki
Y
Y
Ito
T
T
Hagiwara
H
H
Hirose
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA; D010450:Endopeptidases / Q000378:metabolism*; D016232:Endothelins / Q000378:metabolism*; D008565:Membrane Proteins; D008666:Metalloendopeptidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D017466:Receptors, Endothelin; D015183:Restriction Mapping; D016415:Sequence Alignment; D012697:Serine Endopeptidases
D016232:Endothelins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D004247:DNA; D010450:Endopeptidases; D012697:Serine Endopeptidases; C026515:type I signal peptidase; D008666:Metalloendopeptidases