Lactic acid enantiomers: separation by thin-layer chromatography on silica gel plates impregnated with Cu2+.
A simple and fast method for the separation of D- and L-lactic acid enantiomers by thin-layer chromatographic silica gel precoated plates impregnated with Cu(II) is proposed. The two lactic acid enantiomers, dissolved in acetone, are deposed on these plates. D- and L-lactic acids form complexes with Cu(II), with different Rf values, suitable for radiochemical measurements. The real separation was confirmed by enzymatic tests carried out on spots of D and L forms scraped from the chromatographic plates. Total radioactivity of 14C-labeled D- and L-lactic acids was recovered. A Cu/lactic acid complex is evidenced.
Cecchi
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L
Centro per lo Studio Tecnologico, Bromatologico e Microbiologico del Latte, CNR, Milano, Italy.
Malaspina
P
P
D000096:Acetone; D002855:Chromatography, Thin Layer; D003300:Copper; D005782:Gels; D007773:Lactates / Q000737:chemistry*; D019344:Lactic Acid; D012822:Silicon Dioxide; D013237:Stereoisomerism
D005782:Gels; D007773:Lactates; D000096:Acetone; D019344:Lactic Acid; D012822:Silicon Dioxide; D003300:Copper