Fission yeast promoter-probe vectors based on hygromycin resistance.
We have constructed fission yeast vectors that carry either complete or 5'-truncated alleles of the hph gene, encoding hygromycin B phosphotransferase. We show that plasmid-borne hph can be expressed in fission yeast to confer hygromycin resistance. The vectors permit selection or screening in fission yeast for promoter activity of DNA fragments from other species. We used the vectors to identify several genomic sequences from Physarum that provide promoter function in fission yeast.
Burland
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McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Pallotta
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Tardif
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Lemieux
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Dove
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D001483:Base Sequence; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005800:Genes, Fungal; D005822:Genetic Vectors; D006921:Hygromycin B / Q000494:pharmacology*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor); D010804:Physarum / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics / Q000254:growth & development
D015345:Oligonucleotide Probes; D006921:Hygromycin B; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase