title: The molecular biology of eosinophil granule proteins.
abstract: Here, we briefly review the molecular biology of the human eosinophil granule proteins, major basic protein (MBP), eosinophil peroxidase (EPO), eosinophil cationic protein (ECP) and eosinophil-derived neurotoxin (EDN). The nucleotide sequence of MBP cDNA indicates that MBP is translated as a 25.2-kilodalton preproprotein; the mpb gene consists of 6 exons and 5 introns spanning 3.3 kilobases (kb). The approximately 2.1-kb nucleotide sequence of EPO cDNA corresponds to a prosequence, light chain and heavy chain in that order; similarities to other peroxidases suggest the existence of a multigene family. EDN and ECP cDNAs and genes are remarkably similar throughout, suggesting a relatively recent divergence. Promoter regions of the 4 genes show interesting differences and similarities which may be related to differential gene regulation.
authors.affiliation: Department of Immunology, Mayo Clinic and Foundation, Rochester, Minn.
authors.forename: K J
authors.initials: KJ
authors.lastname: Hamann
authors.forename: R L
authors.initials: RL
authors.lastname: Barker
authors.forename: R M
authors.initials: RM
authors.lastname: Ten
authors.forename: G J
authors.initials: GJ
authors.lastname: Gleich
mesh_terms: D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D004247:DNA / Q000032:analysis; D047091:Eosinophil Granule Proteins; D047209:Eosinophil Peroxidase; D047208:Eosinophil-Derived Neurotoxin; D004804:Eosinophils / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000235:genetics*; D009195:Peroxidase / Q000235:genetics; D010544:Peroxidases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012260:Ribonucleases
chemical_list: D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D009498:Neurotoxins; D004247:DNA; D047209:Eosinophil Peroxidase; D010544:Peroxidases; D009195:Peroxidase; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases