[Technique for the microdissection of human high resolution chromosome subsequent polymerase chain reaction and microcloning].
In this paper is reported a simple technique for the microdissection of specific regions of human high resolution chromosome subsequent PCR and microcloning. This technique was successfully used to microdissect 4 chromosomal pieces from the distal one third from band 11.2 to the terminal of the short arm of Y chromosome where the testis determining factor located, PCR 30 cycles, and obtained 3.6 x 10(4) clones. This technique the highlight to establish a gene library of many specific regions of human chromosome hereafter.
Deng
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D003001:Cloning, Molecular; D005784:Gene Amplification; D015723:Gene Library; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D014998:Y Chromosome