Mass spectrometric identification of a phorbol diester 12-O-hexadecanoylphorbol-13-acetate, an Epstein-Barr virus-activating substance, in the soil collected from under Sapium sebiferum.
Soil-extracts collected from the ground from under several Euphorbiaceae plants have been known to possess Epstein-Barr virus (EBV)-activating substances which are thought to be one of the environmental co-factors causing nasopharyngeal carcinoma (NPC) in southern part of China and Burkitt's lymphoma (BL) in tropical Africa. Then, a model experiment aimed at chemical characterization of such active substances was carried out using a soil-extract around Sapium sebiferum, a Japanese representative Euphorbiaceae plant. Chromatographic separation guided by the EBV early antigen (EA) inducing activity gave a highly active fraction. Application of this fraction to desorption chemical ionization mass spectrometry identified a major active substance to be 12-O-hexadecanoylphorbol-13-acetate (HPA), which originally occurs in this plant. The method in this model experiment is suggested to be applicable to other samples from the endemic areas of NPC and BL.
Takeda
N
N
Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Ohigashi
H
H
Hirai
N
N
Koshimizu
K
K
Suzuki
M
M
Tatematsu
A
A
Osato
T
T
Mizuno
F
F
D004854:Herpesvirus 4, Human / Q000187:drug effects*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010944:Plants; D012987:Soil / Q000032:analysis*; D013755:Tetradecanoylphorbol Acetate / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000031:analogs & derivatives; D014775:Virus Activation / Q000187:drug effects
D012987:Soil; C070729:12-O-hexadecanoylphorbol-13-acetate; D013755:Tetradecanoylphorbol Acetate