Evaluation of a synthetic-peptide enzyme-linked immunosorbent assay for immunoglobulin M to human parvovirus B19.
A synthetic peptide corresponding to a part of the virus protein 1-virus protein 2 overlapping region of human parvovirus B19 was used in an indirect enzyme-linked immunosorbent assay. Antibodies of the immunoglobulin (Ig) M class were measured in serum samples from patients with erythema infectiosum and controls. In comparison with an IgM assay using native B19 viral antigen, the peptide antigen assay was 92% sensitive and 87% specific. B19 IgM reactivities were seen in a limited number of children with other viral diseases. Specific IgM reactivities to short synthetic viral peptides have previously been reported only with Epstein-Barr virus. Since other sources of viral antigen are limited, the peptide antigen assay may be a useful alternative for the diagnosis of B19-associated disease in human beings.
Fridell
E
E
Department of Virology, Karolinska Institute, Stockholm, Sweden.
Cohen
B J
BJ
Wahren
B
B
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D008969:Molecular Sequence Data; D010321:Parvoviridae / Q000145:classification / Q000276:immunology*; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000276:immunology; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007075:Immunoglobulin M; D010455:Peptides