title: Characterization of a beta-lactamase produced by Pseudomonas paucimobilis.
abstract: A novel beta-lactamase enzyme produced by a strain of Pseudomonas paucimobilis is described. The enzyme differs from other recorded beta-lactamases from Gram-negative aerobic bacteria. It was constitutive, and had the characteristics of a penicillinase. One single band of beta-lactamase activity at pI 4.6 was seen on iso-electric focusing. The enzyme had a molecular mass of 30 kDa. The beta-lactamase was strongly inhibited by tazobactam, sulbactam and clavulanic acid but not by the thiol residue inhibitors p-chloromercuribenzoate and p-chloromercuriphenylsulphonic acid, or by metallo-enzyme inhibitors. Plasmid DNA was not demonstrable, suggesting that the enzyme was chromosomally encoded.
authors.affiliation: Department of Medical Microbiology, University of Liverpool, Royal Liverpool Hospital, UK.
authors.forename: J E
authors.initials: JE
authors.lastname: Corkill
authors.forename: C A
authors.initials: CA
authors.lastname: Hart
authors.forename: A G
authors.initials: AG
authors.lastname: McLennan
authors.forename: S
authors.initials: S
authors.lastname: Aspinall
mesh_terms: D002511:Cephalosporins / Q000378:metabolism; D002876:Chromosomes, Bacterial; D019818:Clavulanic Acid; D002969:Clavulanic Acids / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D008970:Molecular Weight; D010397:Penicillanic Acid / Q000494:pharmacology; D010403:Penicillin Resistance; D010406:Penicillins / Q000378:metabolism; D011549:Pseudomonas / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D013407:Sulbactam / Q000494:pharmacology; D000078142:Tazobactam; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D002511:Cephalosporins; D002969:Clavulanic Acids; D010406:Penicillins; D065093:beta-Lactamase Inhibitors; D019818:Clavulanic Acid; D010397:Penicillanic Acid; D001618:beta-Lactamases; D013407:Sulbactam; D000078142:Tazobactam