Neurotensin receptors in human meningiomas.
We have investigated the presence of neurotensin receptors in human meningiomas by in vitro binding autoradiography. Ten of the 12 meningiomas tested displayed specific [3H]neurotensin binding. All meningothelial (n = 3) and transitional (n = 5) meningiomas were positive, whereas only 2 of the 4 fibroblastic meningiomas showed measurable concentrations of neurotensin binding sites. Within the tumors, [3H]neurotensin binding was preferentially observed in syncytial areas. Saturation experiments showed that the maximal binding capacity (Bmax) greatly varied among tumors, ranging from low values to more than 290 fmol/mg of protein. All positive tumors had neurotensin binding sites with a dissociation constant (kd) within the nanomolar range and a pharmacological specificity for [3H]neurotensin similar to neurotensin receptors.
Przedborski
S
S
Laboratory of Preclinical Neurosciences, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Levivier
M
M
Cadet
J L
JL
D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000737:chemistry*; D008579:Meningioma / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000032:analysis*; D009496:Neurotensin / Q000378:metabolism*; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter / Q000032:analysis*
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter; D009496:Neurotensin