15-HETE is the main eicosanoid present in mucus of ulcerative proctocolitis.
Prostaglandins, leukotrienes and mono-hydroxy acid products of arachidonic acid were measured in mucus of freshly recovered morning stools of a patient with an exacerbation of ulcerative proctocolitis. Eicosanoids in ether extracts were separated by high performance liquid chromatography and amounts determined by radioimmunoassay. Four hydroxy-eicosatetraenoic acids were detected, of which the most important one was identified as 15-hydroxy eicosatetraenoic acid (530 ng/g mucus). Leukotriene B4 was also present (21 ng/g mucus) and small amounts of immunoreactive leukotriene C4 (less than 0.8 ng/g mucus). The prostaglandins 6-keto-PGF1 alpha and PGE2 and thromboxane B2 were found in amounts of 3.7, 2.0 and 9.2 ng/g mucus, respectively.
Zijlstra
F J
FJ
Department of Pharmacology, Erasmus University, Rotterdam, The Netherlands.
Wilson
J H
JH
D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D003093:Colitis, Ulcerative / Q000378:metabolism*; D015777:Eicosanoids / Q000032:analysis; D005243:Feces / Q000737:chemistry; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis*; D007413:Intestinal Mucosa / Q000378:metabolism; D007975:Leukotriene B4 / Q000378:metabolism; D016859:Lipoxygenase Inhibitors; D008297:Male; D009093:Mucus / Q000737:chemistry*; D011349:Proctitis / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D015777:Eicosanoids; D006893:Hydroxyeicosatetraenoic Acids; D016859:Lipoxygenase Inhibitors; D007975:Leukotriene B4; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid