title: [Modification of intracellular cAMP and cGMP concentration in yeast wild strains and in selected mutants from Saccharomyces cerevisiae as a regulation model for higher eukaryotes].
abstract: The addition of D(+)-glucose (final concentration 50 mM) to a cell suspension of yeasts (wild type and several mutants of the cell cycle, the cAMP-dependent protein kinase system, and a mutant of the adenylate cyclase gene) triggers a rapid increase in the concentrations of cAMP and cGMP in the wild strain. In contrast to cAMP, an increase of cGMP was also found in the mutants. cAMP and cGMP have been characterized as second messengers in eucaryotic cells. Cyclic nucleotide activation of the protein kinases enables them to perform their only known function in eukaryotes, the phosphorylation of substrate proteins. The results, described here by using selected yeast mutants as a model for higher eukaryotes, indicate that there exist two different regulatory systems for the control of the cAMP and cGMP levels.
authors.affiliation: Martin-Luther-Universität Halle (Saale), Germany.
authors.forename: O
authors.initials: O
authors.lastname: Sachse
mesh_terms: D000263:Adenylate Kinase / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D011494:Protein Kinases / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D000242:Cyclic AMP; D011494:Protein Kinases; D000263:Adenylate Kinase; D006152:Cyclic GMP; D005947:Glucose
vernacular_title: Beeinflussung der intrazellulären cAMP- und cGMP-Konzentration im Hefewildstamm und in ausgewählten Mutanten von Saccharomyces cerevisiae als Regulationsmodell für höhere Eukaryoten.