title: Revision of the amino acid sequence of the smallest bc1 complex subunit: use of fast atom bombardment mass spectrometry and mass-analysed ion kinetic energy spectrum analysis.
abstract: We have isolated the smallest bc1 complex subunit from an acidic chloroform/methanol extract of bovine cardiac muscle. The identification of the polypeptide was made possible by classical Edman degradation and amino acid analysis. The measurement of its exact molecular weight by fast atom bombardment mass spectrometry (m/z 6519.8), the characterization of a tryptophan cleavage peptide and pepsic peptides by mass measurements and by mass-analysed ion kinetic energy spectrum analysis allow rectification of the amino acid sequence of the smallest bc1 complex subunit. We found a serine residue instead of Gln 22 and tryptophan residues in place of Ser 34 and Ser 38.
authors.affiliation: Laboratoire de Chimie Organique des Substances Naturelles, URA, CNRS 31, Strasbourg, France.
authors.forename: E
authors.initials: E
authors.lastname: Terzi
authors.forename: P
authors.initials: P
authors.lastname: Boyot
authors.forename: A
authors.initials: A
authors.lastname: Van Dorsselaer
authors.forename: B
authors.initials: B
authors.lastname: Luu
authors.forename: E
authors.initials: E
authors.lastname: Trifilieff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D014450:Electron Transport Complex III / Q000737:chemistry*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D010446:Peptide Fragments; D014450:Electron Transport Complex III