Ocular acyclovir delivery by collagen discs: a mouse model to screen anti-viral agents.
Discs 1.6 mm in diameter trephined from corneal collagen shields were used to deliver acyclovir (ACV) to the cornea of mice inoculated with herpes simplex virus type 1 (HSV-1). In the first minute after application to the cornea, there was a 23% decrease of ACV in the discs. After the first minute, ACV clearance from the discs appeared to be exponential with a half-life of 21 minutes. Treatment given 3 times a day reduced HSV-1 titer in tear film, corneal tissue, and trigeminal ganglia. This animal model should be useful to conserve novel potential anti-viral drugs undergoing initial screening.
Willey
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DE
Department of Neurology, City of Hope National Medical Center, Duarte, CA 91010.
Williams
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Faucett
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C
Openshaw
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H
D000212:Acyclovir / Q000493:pharmacokinetics*; D000818:Animals; D001684:Biological Dressings; D003094:Collagen; D003315:Cornea / Q000378:metabolism* / Q000382:microbiology; D004195:Disease Models, Animal; D004337:Drug Carriers; D004353:Drug Evaluation, Preclinical / Q000379:methods; D007635:Keratitis, Dendritic / Q000188:drug therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus
D004337:Drug Carriers; D003094:Collagen; D000212:Acyclovir