Restriction fragment length polymorphisms and sequence variation within the spaP gene of Streptococcus mutans serotype c isolates.
A restriction fragment length polymorphism study was undertaken to determine the extent and location of heterogeneity within spaP encoding the Mr 185,000 cell surface protein P1 (antigen I/II) of Streptococcus mutans serotype c isolates. The gene was found to be highly conserved except for a central variable (V) region predicted to encode less than 150 amino acids. Sequence analysis identified two V-region variants. These differences were independent of the geographic source of the isolates. Southern analysis using synthetic oligonucleotide probes indicated that nonretention of P1 (I/II) by some isolates is not due to a deletion of the 3'-terminal DNA necessary to encode an intact carboxy terminus.
Brady
L J
LJ
Department of Oral Biology, University of Florida, Gainesville 32610.
Crowley
P J
PJ
Ma
J K
JK
Kelly
C
C
Lee
S F
SF
Lehner
T
T
Bleiweis
A S
AS
D000942:Antigens, Bacterial / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D012150:Polymorphism, Restriction Fragment Length; D013295:Streptococcus mutans / Q000235:genetics*
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D004269:DNA, Bacterial; D015345:Oligonucleotide Probes