title: Microdissection of human high resolution banded chromosome, polymerase chain reaction and microcloning.
abstract: A simple technique for microdissection of specific region of human high resolution banded chromosome, followed by polymerase chain reaction (PCR), and microcloning was successfully used to microdissect 4 chromosomal pieces from the distal one third from band 11.2 to the terminal of the short arm of Y chromosome where the testis determining factor is located; 3.6 x 10(4) clones were obtained after 30 cycles of PCR. We analysed 41 clones with insert. The size of insert ranges from 140 to 350 bp (average 250 bp). A Southern blot analysis was done for one of them, and a 2.5 kb Hind III fragment was detected.
authors.affiliation: National Laboratory of Medical Genetics, Hunan Medical University, Changsha.
authors.forename: H X
authors.initials: HX
authors.lastname: Deng
authors.forename: X X
authors.initials: XX
authors.lastname: He
authors.forename: L Y
authors.initials: LY
authors.lastname: Li
authors.forename: J H
authors.initials: JH
authors.lastname: Xia
authors.forename: N
authors.initials: N
authors.lastname: Niikawa
mesh_terms: D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004259:DNA-Directed DNA Polymerase; D004210:Dissection; D005821:Genetic Techniques; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase; D014998:Y Chromosome
chemical_list: D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase