Enhancement of human neutrophil responses to platelet activating factor by 5(S)-hydroxy-eicosatetraenoate.
5(S)-hydroxy-eicosatetraenoate (5(S)-HETE) enhanced the ability of platelet-activating factor (PAF) to stimulate neutrophil inositol phospholipid turnover, Ca2+ transients, superoxide anion generation, and degranulation. It did not alter responses to leukotriene B4, N-formyl-methionylleucylphenylalanine, or ionomycin. Moreover, 5(R)- and 15(S)-HETE had little effect on PAF. 5(S)-HETE thus acted stereospecifically and stimulus-selectively to potentiate early occurring transductional events as well as later occurring functional responses to PAF. The HETE may influence the actions of PAF by up-regulating PAF receptors and/or these receptors' linkages with the G-protein/phospholipase C axis.
Rossi
A G
AG
Department of Medicine, Wake Forest University Medical Center, Winston Salem, NC 27103.
Redman
J F
JF
Jacobson
D P
DP
O'Flaherty
J T
JT
D004075:Diglycerides / Q000378:metabolism; D004357:Drug Synergism; D006801:Humans; D006868:Hydrolysis; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism / Q000494:pharmacology*; D007295:Inositol Phosphates / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000378:metabolism; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism
D004075:Diglycerides; D006893:Hydroxyeicosatetraenoic Acids; D007295:Inositol Phosphates; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D010972:Platelet Activating Factor; D013481:Superoxides; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid