title: Receptor-mediated changes in intracellular [Ca2+] in cultured rat brain capillary endothelial cells.
abstract: Agents which are known to alter blood-brain barrier permeability have been tested for their ability to raise [Ca2+]i in rat brain capillary endothelial cells loaded with the calcium indicator Fura-2, and subjected to dual wavelength excitation. The cells show increases in [Ca2+]i in response to bradykinin, histamine and ATP. Possible sources of this calcium are discussed.
authors.affiliation: Biomedical Sciences Division, King's College London, U.K.
authors.forename: P A
authors.initials: PA
authors.lastname: Revest
authors.forename: N J
authors.initials: NJ
authors.lastname: Abbott
authors.forename: J I
authors.initials: JI
authors.lastname: Gillespie
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D001812:Blood-Brain Barrier; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002196:Capillaries; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology*; D007700:Kinetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology*; D011983:Receptors, Purinergic / Q000187:drug effects / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology
chemical_list: D000227:Adenine Nucleotides; D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; D012701:Serotonin; D006632:Histamine; D001920:Bradykinin; D002118:Calcium