Interleukin 4 inhibits murine osteoclast formation in vitro.
Interleukin 4 (IL-4) is a product of activated T cells and mast cells with effects on immunologic and hematopoietic processes. We now report that IL-4 inhibits the formation of osteoclasts from murine bone marrow cells cocultured with stromal cells. Numerous (3,000-4,000 cells/2 cm2) tartrate-resistant acid-phosphatase-positive multinucleated cells with the capacity to generate cAMP in response to salmon calcitonin (ED50 = 10(-10) M) developed within 10-12 days of culture. IL-4 (ID50 = 10 U/ml) inhibited osteoclast generation in doses similar to those that induce proliferation of IL-4-responsive T cells. Additionally, the rat antimurine IL-4 monoclonal antibody 11B11 antagonizes the IL-4-inhibitory effect on osteoclast formation. These results suggest that IL-4 impedes agonist-induced in vitro bone resorption by inhibiting osteoclastogenesis.
Shioi
A
A
Department of Pathology, Jewish Hospital of St. Louis, Washington University, Missouri 63110.
Teitelbaum
S L
SL
Ross
F P
FP
Welgus
H G
HG
Suzuki
H
H
Ohara
J
J
Lacey
D L
DL
D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D016723:Bone Remodeling / Q000502:physiology; D002116:Calcitonin / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000502:physiology; D051379:Mice; D010010:Osteoclasts / Q000187:drug effects*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D015847:Interleukin-4; D002116:Calcitonin; D000242:Cyclic AMP