Epigenetic control of tumor cell morphology.
XC cell line derived from a single rat cell transformed by the Prague strain of Rous sarcoma virus produced morphologically different colonies. Among them, two distinct cell types consisting of thick, fusiform cells (L-type), and of flat, polygonal cells (R-type) were apparent. By repeated subclonings, pure cultures, L1 and R1, respectively, were obtained. These clones underwent morphological conversion during prolonged culture; L-type colonies appeared in the R-type clone and vice versa. The kinetic curve suggested that the conversion was multi-stepped. When inoculated into nude mice, L-type cells produced much larger tumors at a higher frequency than R-type cells, and the tumors induced by these two clones were histologically different. The expression of v-src gene was higher in L-type than in R-type cells at both mRNA and protein levels.
Horio
K
K
Department of Bacteriology, Faculty of Medicine, University of Tokyo.
Yoshikura
H
H
Kawabata
M
M
Odawara
T
T
Sudo
K
K
Fujitani
Y
Y
Lee
G
G
Iwamoto
A
A
D000199:Actins / Q000032:analysis; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D015139:Blotting, Southern; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D003598:Cytoskeletal Proteins / Q000032:analysis; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D008745:Methylation; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D051381:Rats; D015183:Restriction Mapping; D001357:Sarcoma, Avian / Q000235:genetics / Q000473:pathology*; D014183:Transplantation, Heterologous; D016596:Vinculin
D000199:Actins; D003598:Cytoskeletal Proteins; D004273:DNA, Neoplasm; D016596:Vinculin