VLA-2 is a collagen receptor on endothelial cells.
In order to identify cell-substrate adhesion receptors on vascular endothelium, murine monoclonal antibodies (MoAb) were raised against human umbilical vein endothelial cells (HUVE). One anti-HUVE MoAb, RMAC11, identified the adhesion receptor VLA-2 as it immunoprecipitated a non-covalently linked heterodimer of 160 kD and 130 kD, which was identical to the heterodimer immunoprecipitated by the anti-VLA-2 MoAb, 12F1 and 5E8. Furthermore, proteolytic peptide maps of the VLA-2 alpha- and beta-chains were highly homologous with those of the RMAC11-recognized molecule. However, unlike other VLA-2 MoAb, RMAC11 also identified an 85 kD band which migrated to 90 kD under reducing conditions. This band was most likely a fragment of the 160 kD alpha-chain as a similar alpha-chain derived fragment has been demonstrated in the immunoprecipitates of some VLA-4 reactive monoclonals. However the possibility that this may be a novel molecule associated with VLA-2 has not been excluded. In vitro assays of HUVE adhesion to collagen types 1 and 4, laminin and fibronectin showed that RMAC11 blocked adhesion to collagen (types 1 and 4) and laminin, but had no effect on HUVE adhesion to fibronectin, confirming that VLA-2 is a collagen and laminin receptor for HUVE.
O'Connell
P J
PJ
Department of Nephrology, Royal Melbourne Hospital, Victoria, Australia.
Faull
R
R
Russ
G R
GR
D'Apice
A J
AJ
D000911:Antibodies, Monoclonal; D002448:Cell Adhesion / Q000276:immunology; D003094:Collagen / Q000276:immunology; D004730:Endothelium, Vascular / Q000276:immunology*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007797:Laminin / Q000276:immunology; D010449:Peptide Mapping; D011233:Precipitin Tests; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000276:immunology; D038981:Receptors, Collagen; D016029:Receptors, Very Late Antigen / Q000737:chemistry* / Q000276:immunology; D014470:Umbilical Cord / Q000276:immunology
D000911:Antibodies, Monoclonal; D007797:Laminin; D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; D016029:Receptors, Very Late Antigen; D003094:Collagen