Human papillomavirus type-related DNA and c-myc oncogene alterations in colon cancer cell lines.
Although squamous-cell epithelium is the most frequent target site of human papillomavirus (HPV) infection, a similar infection is demonstrated in columnar epithelial cells in this paper. The papillomavirus expression in three cell lines was detected in colorectal adenocarcinoma of Chinese patients. The HPV-16 and HPV-18 DNA sequences were found in colorectal cancer cell lines, which might suggest the correlation of HPV to the etiology of colorectal cancers. In addition, c-myc oncogene was identified by amplification in all three colorectal cancer cell lines, but only normal germ-line fragments were found in control tissue. The correlation between HPV and c-myc, and the implications of these findings in colorectal cancers are also discussed.
Cheng
J Y
JY
Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.
Meng
C L
CL
Chao
C F
CF
Gau
S D
SD
Lin
J C
JC
D000230:Adenocarcinoma / Q000235:genetics* / Q000382:microbiology*; D001345:Autoradiography; D015152:Blotting, Northern; D015139:Blotting, Southern; D003110:Colonic Neoplasms / Q000235:genetics* / Q000382:microbiology*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D015246:Deoxyribonuclease EcoRI; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D014407:Tumor Cells, Cultured
D015344:DNA Probes, HPV; D004279:DNA, Viral; D015246:Deoxyribonuclease EcoRI