title: VIP stimulation of polarized macromolecule secretion in cultured chick embryonic retinal pigment epithelium.
abstract: Vasoactive intestinal peptide (VIP) stimulated macromolecule secretion at the apical membranes of the chick embryonic retinal pigment epithelium cultured on permeable supports in a time- and concentration-dependent manner. VIP stimulated secretion of molecules with MW of 80, 74, 70, 60, 42, 35, 24, 20, and 14 kDa. A 1.9- to 2.6-fold stimulation in secretion of molecules with MW greater than 10 kDa precipitable by 10% trichloroacetic acid was observed after treatment with 1 microM VIP for 15 min. The effect of 1 microM VIP was mimicked by 10 microM dibutyryl cyclic AMP and attenuated by dopamine (1 x 10(-4) M), while colchicine, beta-lumicolchicine, and monensin, all at 1 microM, had no effect.
authors.affiliation: Department of Ophthalmology, University of Maryland, Baltimore 21201.
authors.forename: S W
authors.initials: SW
authors.lastname: Koh
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology; D004298:Dopamine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D008162:Lumicolchicines / Q000494:pharmacology; D046911:Macromolecular Substances; D008985:Monensin / Q000494:pharmacology; D010539:Permeability; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D012168:Retinal Pigments / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000502:physiology*
chemical_list: D008162:Lumicolchicines; D046911:Macromolecular Substances; D012168:Retinal Pigments; D014660:Vasoactive Intestinal Peptide; D008985:Monensin; D000242:Cyclic AMP; D003078:Colchicine; D004298:Dopamine