title: Characterization and mapping of microdissected genomic clones from the adenomatous polyposis coli (APC) region.
abstract: The gene associated with adenomatous polyposis coli (APC) has been mapped to the long arm of chromosome 5. To saturate the APC region with DNA markers, two independent microdissection libraries with an emphasis on 5q21.2-21.3 and 5q22 have been constructed from GTG-banded human metaphase chromosomes. PCR-amplified insert DNA of the primary amplificate used as a probe in chromosomal in situ suppression (CISS) hybridization of human metaphase spreads revealed region-specific signals at the chromosomal site that was excised for cloning. One hundred forty-two inserts, derived from both libraries, have been characterized in more detail. Deletion mapping analysis was performed with 17 single-copy clones on a hamster-human hybrid cell panel. Seven of these clones were located within two interstitial deletions of 6-8 Mb from APC-affected individuals around chromosome bands 5q21-22. The identification of new microclones mapping into these deletions and their use in isolating YAC clones should contribute to the construction of a contiguous physical map of the APC region.
authors.affiliation: Imperial Cancer Research Fund, London, United Kingdom.
authors.forename: G
authors.initials: G
authors.lastname: Hampton
authors.forename: G
authors.initials: G
authors.lastname: Leuteritz
authors.forename: H J
authors.initials: HJ
authors.lastname: Lüdecke
authors.forename: G
authors.initials: G
authors.lastname: Senger
authors.forename: U
authors.initials: U
authors.lastname: Trautmann
authors.forename: H
authors.initials: H
authors.lastname: Thomas
authors.forename: E
authors.initials: E
authors.lastname: Solomon
authors.forename: W F
authors.initials: WF
authors.lastname: Bodmer
authors.forename: B
authors.initials: B
authors.lastname: Horsthemke
authors.forename: U
authors.initials: U
authors.lastname: Claussen
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5 / Q000648:ultrastructure; D003001:Cloning, Molecular / Q000379:methods; D004252:DNA Mutational Analysis; D015342:DNA Probes; D015698:Genomic Library; D006651:Histocytochemistry; D006801:Humans; D007621:Karyotyping; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes