Evaluation of an amidolytic test for comparative calibration of HMW- and LMW-heparins.
Amidolytic chromogenic substrate assays are frequently used to determine the anticoagulant activities of various commercial heparins. With the help of a combined assay method heparin characterization is made possible using the TAT/XAT quotient under consideration of the simultaneous inhibition of the two serine proteases thrombin and factor Xa by antithrombin III. The test is primarily designed for qualitative characterization where a numerical value can be assigned to every heparin. However, quantitative aspects may also be evaluated.
Münstedt
K
K
Institute for Clinical Chemistry and Pathobiochemistry, Justus-Liebig-University Giessen, Federal Republic of Germany.
Bleyl
H
H
Róka
L
L
D000577:Amides / Q000378:metabolism*; D000990:Antithrombin III / Q000378:metabolism; D000991:Antithrombins / Q000378:metabolism; D002138:Calibration; D006493:Heparin / Q000494:pharmacology*; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D006801:Humans; D010447:Peptide Hydrolases / Q000378:metabolism
D000577:Amides; D000991:Antithrombins; D006495:Heparin, Low-Molecular-Weight; C046193:antithrombin III-protease complex; D000990:Antithrombin III; D006493:Heparin; D010447:Peptide Hydrolases