Modulation of the free sphingosine levels in Epstein Barr virus transformed human B lymphocytes by phorbol dibutyrate.
The amounts of free sphingosine in Epstein Barr virus transformed B lymphocytes (EBV-B) treated with sphingosine and phorbol-12,13-dibutyrate (PD) has been quantified by high performance liquid chromatography (HPLC). PD treatment did not affect intracellular sphingosine level, while it seems to lessen the removal of this long chain base in sphingosine-treated EBV-B cells. The previous results relative to sphingosine-dependent changes in choline-metabolite levels have to be interpreted on the basis of these results.
Miccheli
A
A
Department of Chemistry, University La Sapienza, Rome, Italy.
Ricciolini
R
R
Lagana
A
A
Piccolella
E
E
Conti
F
F
D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D013110:Sphingosine / Q000378:metabolism* / Q000494:pharmacology
D015240:Phorbol 12,13-Dibutyrate; D013110:Sphingosine