[A comparison study of immunohistochemical detection of ras P21 and N-ras mRNA in situ hybridization in hepatocellular carcinoma and pericancerous nontumor liver tissue].
30 cases of hepatocellular carcinoma and pericancerous nontumor samples were studied by examining the serial sections for immunohistochemical detection of P21 product accompanying identification of N-ras mRNA by in situ hybridization with biotinylated N-ras cDNA probe and a significant correlation was noticed between the results obtained (P less than 0.02). It was suggested that immunohistochemical detection of ras oncogene P21 product may indicate the expression of ras oncogene. The higher expression of N-ras mRNA of the carcinoma and the non-tumor tissue peripheral to the cancer by in situ hybridization were 53.33% and 28.57% respectively. The positive ratio of P21 were 96.67% and 90.48%.
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West China University of Medical Sciences, Chengdu.
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D015342:DNA Probes; D015870:Gene Expression; D011905:Genes, ras; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D015689:Oncogene Protein p21(ras) / Q000032:analysis*; D012333:RNA, Messenger
D015342:DNA Probes; D012333:RNA, Messenger; D015689:Oncogene Protein p21(ras)