Tumor suppression by chromosome 11 is not due to cellular senescence.
Previous hybrid studies involving fusion of normal with immortal human cells indicated that the phenotype of cellular senescence is dominant and that immortality results from recessive changes in normal growth regulatory genes. We have further assigned 28 different immortal human cell lines to at least four complementation groups for indefinite division. In order to identify the chromosomes involved in regulating cell proliferation, we have introduced single human chromosomes by microcell fusion into immortal human cells representative of the different complementation groups. Our results demonstrate that the introduction of chromosome 11, implicated in tumor suppression, does not cause cellular senescence in three different immortal human cell lines tested.
Ning
Y
Y
Roy M. and Phyllis Gough Huffington Center on Aging, Baylor College of Medicine, Houston, Texas 77030.
Shay
J W
JW
Lovell
M
M
Taylor
L
L
Ledbetter
D H
DH
Pereira-Smith
O M
OM
D000818:Animals; D002455:Cell Division / Q000235:genetics; D002459:Cell Fusion; D002470:Cell Survival / Q000235:genetics*; D002478:Cells, Cultured; D002880:Chromosomes, Human, Pair 11; D003582:Cytogenetics; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length