title: Investigation of a chronic feed-passage problem on a broiler farm in northwest Arkansas.
abstract: A commercial broiler farm with a history of poor feed conversion and chronic feed-passage problems was chosen for investigation. Chickens were taken from the broiler flock at specified intervals during growout and tested by virus isolation and enzyme-linked immunosorbent assay (ELISA) for avian reovirus. Abnormal tissue pathology was first seen in the broilers at 9 days of age and continued sporadically throughout the growout period. Antireovirus antibody levels began to increase at 24 days of age. Avian reovirus and avian adenovirus was recovered at different intervals starting at 17 and 31 days of age, respectively. One-day-old specific-pathogen-free chicks housed in filtered-air positive-pressure isolation units were inoculated with two inocula recovered from the field study. Avian reovirus was recovered from the tissues of both treatment groups using chick kidney cells. Significant weight differences were seen in one of the two treatment groups. This avian reovirus was given the name SS-412.
authors.affiliation: Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville 72701.
authors.forename: R O
authors.initials: RO
authors.lastname: Apple
authors.forename: J K
authors.initials: JK
authors.lastname: Skeeles
authors.forename: G E
authors.initials: GE
authors.lastname: Houghten
authors.forename: J N
authors.initials: JN
authors.lastname: Beasley
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001131:Arkansas; D000259:Aviadenovirus / Q000302:isolation & purification; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005767:Gastrointestinal Diseases / Q000382:microbiology / Q000662:veterinary*; D011201:Poultry Diseases / Q000382:microbiology*; D012087:Reoviridae / Q000276:immunology / Q000302:isolation & purification*; D012088:Reoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms
chemical_list: D000914:Antibodies, Viral