A short 5' region of the long terminal repeat is required for regulation by hormone and heat shock of Drosophila retrotransposon 1731.
1731, a Drosophila retrotransposon was first described as having a transcription activity which was negatively regulated by 20-hydroxyecdysone (20-OH), the steroid molting hormone of insects. Using constructions expressing the bacterial chloramphenicol-acetyltransferase (CAT) gene under the control of the entire or deleted Long Terminal Repeats (LTRs) of 1731, we were able to show that a short (28 bp) sequence located in the U3 region of these LTRs was required for 1) the increase in promoter strength, 2) negative regulation by 20-OH and, 3) positive regulation by heat shock.
Ziarczyk
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P
Groupe de Génétique Cellulaire et Moléculaire, URA-CNRS 1135, Université Pierre et Marie Curie, Paris, France.
Best-Belpomme
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M
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics; D004441:Ecdysterone / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection
D004251:DNA Transposable Elements; D006360:Heat-Shock Proteins; D004441:Ecdysterone; D004247:DNA