The identification and cloning of the murine genes encoding the liver specific F alloantigens.
The liver specific F alloantigen is a highly conserved abundant protein found in hepatic cytoplasm; smaller amounts are detected in renal tubule cells and the perikaryon cells of the central nervous system. Although the biological function of the F alloantigen is unknown, the immune response to F has been extensively studied as a murine model of tolerance and autoimmunity. Murine F exists in two allelic forms, designated F type 1 and type 2, each of approximately 43 kDa. The immune response to the allotypic form is restricted to mouse strains of I-Ak. Responding strains immunized with allotypic F break tolerance and produce precipitating antibody that reacts with both allelic forms, i.e., immunogen and self. Thus an autoantibody is produced. Using the previously isolated rat F cDNA as a probe, we report the cloning and sequencing of the two murine F allotypes. These two alleles are nearly homologous except at the extremes of the coding sequence. There are a number of regions within the F sequence that are similar to peptides that interact specifically with I-Ak. In particular, there is a sequence near the carboxy terminus, where the two allotypes differ, that has homology to the I-Ak restricted malarial antigen peptide of the ring-infected erythrocyte surface antigen (RESA).
Teuber
S S
SS
Division of Clinical Immunology, University of California, Davis 95616.
Coppel
R L
RL
Ansari
A A
AA
Leung
P S
PS
Neve
R
R
Mackay
I R
IR
Gershwin
M E
ME
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D015342:DNA Probes; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D007114:Immunization; D007519:Isoantigens / Q000235:genetics* / Q000302:isolation & purification; D008099:Liver / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D011993:Recombinant Fusion Proteins; C030497:liver specific F antigen