Regulation of eicosanoid release in human umbilical endothelial cells.
The concentration dependent influence of adenine nucleotides, thrombin and ionophore A 23187 on eicosanoid-production of human endothelial cells was investigated. The prostaglandin I2 (PGI2)-and thromboxane A2 (TXA2)-release were highest at concentrations of 1 mmol/l and 4 mmol/l adenine nucleotides, respectively. TXA2-release decreased at higher concentrations. Ionophore-induced eicosanoid-formation reached its maximum at 15 mumol/l for both PGI2 and TXA2. Thrombin stimulation resulted in a bell shaped concentration dependency, whereby most TXA2 was released at a concentration of 1 U/ml and most PGI2 at 5 U/ml. Intracellular cAMP showed significant (p less than 0.01) increases stimulating cells with ADP- and ATP-concentrations higher than 500 mumol/l, whereas ionophore and thrombin did hardly affect cAMP levels.
Weigel
G
G
2nd Dept. of Surgery, University of Vienna, Austria.
Griesmacher
A
A
Müller
M M
MM
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D012636:Secretory Rate / Q000187:drug effects; D013268:Stimulation, Chemical; D013917:Thrombin / Q000494:pharmacology; D013928:Thromboxane A2 / Q000378:metabolism*; D013929:Thromboxane B2 / Q000032:analysis; D014471:Umbilical Veins
D000001:Calcimycin; D013929:Thromboxane B2; D013928:Thromboxane A2; D015121:6-Ketoprostaglandin F1 alpha; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D011464:Epoprostenol; D000242:Cyclic AMP; D013917:Thrombin