Panagrellus redivivus: failure to find evidence for the occurrence and biosynthesis of sialic acids.
The occurrence of sialic acids in the free-living nematode Panagrellus redivivus was studied by periodate oxidation/[3H]sodium borohydride reduction of about 10(7) nematodes. In parallel, the capability of sialic acid biosynthesis was examined by metabolic labeling of the same number of nematodes with N-[3H]acetylmannosamine. In both experiments, radioactivity was incorporated into the nematodes. Mild acid hydrolysis, however, did not release radioactively labeled sialic acids or derivatives as tested by radio thin-layer chromatography, suggesting that P. redivivus does not contain or synthesize sialic acids.
Reuter
G
G
Biochemisches Institut, Universität Kiel, Federal Republic of Germany.
Aumann
J
J
Wyss
U
U
Jansson
H B
HB
Schauer
R
R
D000818:Animals; D001894:Borohydrides; D006595:Hexosamines / Q000378:metabolism; D009348:Nematoda / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D010504:Periodic Acid; D012794:Sialic Acids / Q000032:analysis* / Q000096:biosynthesis
D001894:Borohydrides; D006595:Hexosamines; D012794:Sialic Acids; D010504:Periodic Acid; C025364:sodium borohydride; C002022:N-acetylmannosamine