Screening rabbit colonies for antibodies to Pasteurella multocida by an ELISA.
Rabbit serum samples from eleven different research facilities were evaluated for the presence of immunoglobulin G against Pasteurella multocida by using an enzyme-linked immunosorbent assay (ELISA). Each facility which submitted serum samples also provided a brief history of each rabbit colony tested. Rabbits from colonies reported to have endemic P. multocida or of undetermined status had 83 (58.9%) of 141 rabbits that were positive. Colonies reported to be free from P. multocida had 110 (92.4%) of 119 rabbits that were negative by ELISA. The ELISA test described here showed a high degree of agreement (92-94%) with two other P. multocida ELISAs at different diagnostic facilities. This study confirms that an ELISA testing for serum antibodies against the P. multocida is a reliable diagnostic tool to screen colonies for P. multocida.
Zaoutis
T E
TE
Laboratory Animal Research Center, Rockefeller University, New York, NY 10021-6399.
Reinhard
G R
GR
Cioffe
C J
CJ
Moore
P B
PB
Stark
D M
DM
D000818:Animals; D000830:Animals, Laboratory / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D008403:Mass Screening; D010326:Pasteurella Infections / Q000175:diagnosis / Q000517:prevention & control; D016979:Pasteurella multocida / Q000276:immunology*; D011817:Rabbits / Q000382:microbiology*
D000907:Antibodies, Bacterial