[Ischemia/reperfusion damage of the liver caused by free radicals--direct radical detection using electron spin resonance (ESR)].
The generation of free oxygen radicals is presumed to be a pathogenetic principle in various conditions, primarily in postischemic reperfusion injury. Their assessment is difficult. ESR is an excellent tool to assess free radicals directly. In an experimental model of rat liver ischemia and reperfusion the increased generation of free radicals during reperfusion in liver tissue could be demonstrated after 60 min of liver ischemia. Elevated production rates of radicals could be detected after 5 min of reperfusion for at least 45 min with a maximum after 15 min of reperfusion. Scavenging of these radicals has to start in the very beginning of reperfusion.
Kunz
R
R
Abteilung für Allgemeine Chirurgie, Universität Ulm, Bundesrepublik Deutschland.
Brune
H A
HA
Ziegler
U
U
Marzinzig
M
M
Beger
H G
HG
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000032:analysis; D006878:Hydroxides / Q000032:analysis; D017665:Hydroxyl Radical; D007511:Ischemia / Q000503:physiopathology*; D008099:Liver / Q000098:blood supply*; D008297:Male; D010101:Oxygen Consumption / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000503:physiopathology*; D013481:Superoxides / Q000032:analysis
D005609:Free Radicals; D006878:Hydroxides; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide
Ischämie/Reperfusionsschaden der Leber durch freie Radikale--direkter Radikalnachweis mittels Elektronenspinresonanz (ESR).