Rapid techniques for diagnostic and epidemiological studies of rotavirus infection.
Two rapid procedures modifying the polyacrylamide gel electrophoresis (PAGE) standard technique for the diagnosis of acute gastroenteritis caused by rotavirus infections are described. These assays permit the detection of both group A and non-group-A rotaviruses, and can be employed in most microbiology laboratories. The cost per sample is minimal compared with that of enzyme immune assays (ELISA) or the agglutination tests available commercially.
Giordano
M O
MO
Instituto de Virologia Dr. J.M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Argentina.
Basnec
S N
SN
Nates
S V
SV
Bennun
F
F
Depetris
A R
AR
D001118:Argentina / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology*; D006801:Humans; D012367:RNA, Viral / Q000302:isolation & purification; D012401:Rotavirus / Q000737:chemistry*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000453:epidemiology*
D012367:RNA, Viral