title: Modulation of A1 adenosine receptor function in rat brain by the polyamine, spermine.
abstract: Studies on factors modulating the binding of agonist ligands to A1 adenosine receptors in rat forebrain membranes revealed that the reduction of [3H]cyclohexyladenosine [( 3H]CHA) binding, observed after removing Mg2+ by pretreatment with ethylene-dinitrilo-tetraacetic acid (EDTA), was restored by the polyamine, spermine (1mM). Parallel electrophysiological experiments performed on rat hippocampal slices in Mg(2+)-free medium indicated that spermine also led to a recovery of the depressive effect of 1 microM adenosine on stimulus train-evoked neuronal Ca2+ influx. These observations suggest that the polyamine, spermine is, like Mg2+, able to control the physiological adenosine-mediated modulation of synaptic transmission by changing the affinity state of A1 receptors.
authors.affiliation: Department of Neuromorphology, Max-Planck-Institute of Psychiatry, Martinsreid, F.R.G.
authors.forename: R
authors.initials: R
authors.lastname: Wasserkort
authors.forename: E
authors.initials: E
authors.lastname: Hoppe
authors.forename: M
authors.initials: M
authors.lastname: Reddington
authors.forename: P
authors.initials: P
authors.lastname: Schubert
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology; D004594:Electrophysiology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011983:Receptors, Purinergic / Q000187:drug effects*; D013096:Spermine / Q000494:pharmacology*; D009435:Synaptic Transmission / Q000187:drug effects; D014970:Xanthines / Q000494:pharmacology
chemical_list: D011983:Receptors, Purinergic; D014970:Xanthines; D013096:Spermine; C027513:N(6)-cyclohexyladenosine; D004492:Edetic Acid; C051360:1,3-dipropyl-8-cyclopentylxanthine; D008274:Magnesium; D000241:Adenosine