Inorganic-pyrophosphate-dependent phosphorylation of spinach thylakoid proteins.
It has been shown for the first time that several photosystem-II thylakoid proteins and the main chlorophyll-a/b light-harvesting complex can be phosphorylated with inorganic pyrophosphate as phosphate donor. With pyrophosphate, as with ATP, the protein-kinase reaction is dependent on light or a strong reducing agent. The reaction which can be demonstrated in well-washed spinach thylakoids is dependent on electron transport and is controlled by the redox state of the plastoquinone pool. It is suggested that the pyrophosphate-dependent thylakoid protein phosphorylation is mediated by the same kinase which is responsible for the ATP-dependent protein phosphorylation. This pyrophosphate-dependent kinase activity may be derived from an evolutionary precursor from which ATP-dependent protein phosphorylation also developed.
Pramanik
A M
AM
Department of Biochemistry, Arrhenius Laboratories, Stockholm University, Sweden.
Bingsmark
S
S
Lindahl
M
M
Baltscheffsky
H
H
Baltscheffsky
M
M
Andersson
B
B
D000255:Adenosine Triphosphate / Q000378:metabolism; D016227:Benzoquinones / Q000494:pharmacology; D011756:Diphosphates / Q000378:metabolism*; D004237:Diuron / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins / Q000378:metabolism*; D008773:Methylphenazonium Methosulfate / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism; D045332:Photosystem II Protein Complex; D010944:Plants / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors
D016227:Benzoquinones; D011756:Diphosphates; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C021261:thylakoid polypeptides; D008773:Methylphenazonium Methosulfate; C029322:phenylbenzoquinone; D000255:Adenosine Triphosphate; D004237:Diuron; D011494:Protein Kinases; D006180:Proton-Translocating ATPases