An Epstein-Barr virus-producer line Akata: establishment of the cell line and analysis of viral DNA.
An Epstein-Barr virus (EBV)-producer line, designated Akata, was established from a Japanese patient with Burkitt's lymphoma. The Akata line possessed the Burkitt's-type chromosome translocation, t(8q- ; 14q+), and was derived from the tumor cell. Akata cells produced a large quantity of transforming virus upon treatment of cells with anti-immunoglobulin antibodies (Takada, 1984). Southern blot analysis of viral DNA indicated that the Akata EBV is nondefective and more representative of wild-type viruses. Akata cells should be useful as a source of EBV.
Takada
K
K
Department of Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Horinouchi
K
K
Ono
Y
Y
Aya
T
T
Osato
T
T
Takahashi
M
M
Hayasaka
S
S
D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D015139:Blotting, Southern; D002051:Burkitt Lymphoma / Q000276:immunology / Q000382:microbiology*; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D019309:Epstein-Barr Virus Nuclear Antigens; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007074:Immunoglobulin G