New oligopeptide immunoglobulin G test for human parvovirus B19 antibodies.
A new, highly sensitive and specific enzyme immunoassay using oligopeptides as antigen (enzyme-linked immunosorbent assay [ELISA] B19-OP) for detecting parvovirus B19-specific immunoglobulin G (IgG) was established. As antigens, B19-specific oligopeptides of 24 and 30 kDa derived from a 196-kDa fusion protein of beta-galactosidase and viral capsid protein (VPI) of B19 after CNBr cleavage and separation by high-pressure liquid chromatography were used. Of 139 serum specimens tested in parallel for anti-B19 IgG by standard ELISA using B19 particles as antigen and by ELISA B19-OP, 73 (52.5%) were positive and 63 (45.3%) were negative in both tests, and 3 (2.2%) were negative by standard ELISA but positive by ELISA B19-OP and by immunoblot. By using ELISA B19-OP, it was possible to detect anti-B19 IgG in an asymptomatic blood donor 4 weeks after acute infection, and anti-B19 IgG titers of 10(-5) could be measured in convalescent-phase sera.
Schwarz
T F
TF
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig-Maximilians-University of Munich, Federal Republic of Germany.
Modrow
S
S
Hottenträger
B
B
Höflacher
B
B
Jäger
G
G
Scharti
W
W
Sumazakl
R
R
Wolf
H
H
Middeldorp
J
J
Roggendorf
M
M
D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D009842:Oligopeptides / Q000276:immunology; D010321:Parvoviridae / Q000276:immunology*; D010322:Parvoviridae Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D009842:Oligopeptides