title: Induction of chromosome abnormalities in mouse and human epidermal keratinocytes by the human papillomavirus type 16 E7 oncogene.
abstract: Cytogenetic abnormalities associated with human papillomavirus (HPV) type 16 were studied using primary human and mouse epidermal keratinocytes. The E7 transforming gene of HPV-16 was found to induce chromosome duplication in epidermal keratinocytes; little or no detectable chromosome disorganization was associated with the function of the E6 gene. These results suggest that the E7 gene-linked cytogenetic effect reflects HPV-16-associated pathogenicity in the early phase of transformation.
authors.affiliation: Laboratory of Molecular and Cell Biology, Kanagawa Cancer Center Research Institute, Yokohama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hashida
authors.forename: S
authors.initials: S
authors.lastname: Yasumoto
mesh_terms: D000782:Aneuploidy; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003001:Cloning, Molecular; D004171:Diploidy; D000078404:Epidermal Cells; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D006801:Humans; D007621:Karyotyping; D015603:Keratinocytes / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics* / Q000472:pathogenicity; D050725:Papillomavirus E7 Proteins; D010957:Plasmids; D011123:Polyploidy; D012367:RNA, Viral / Q000032:analysis; D012097:Repressor Proteins; D014162:Transfection
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012367:RNA, Viral; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16