Hepatocyte cell lines established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.
To establish cell lines exhibiting differentiation phenotypes, the immortalized cell lines were rapidly established from the primary culture of different tissues of transgenic mice harboring SV40 temperature-sensitive large T-antigen gene. The established cell lines grew at permissive temperature (33 degrees C), but not at nonpermissive temperature (39 degrees C). Several different cell types could be rapidly immortalized and cloned from the adult transgenic mice tissues. Among those cell lines, the established hepatocyte cell lines (TLR cell lines) exhibited liver-specific morphological and biochemical properties, but their properties were not coupled with the growth condition modified by temperature. The hepatocyte cell lines showed an inducibility of P450IA1 by 3-methylcholanthrene as observed in rat livers and this liver-specific function was stable even after 6 months of culture by continuous passages.
Yanai
N
N
Department of Cell Biology, Tohoku University, Sendai, Japan.
Suzuki
M
M
Obinata
M
M
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D015152:Blotting, Northern; D002455:Cell Division; D002460:Cell Line; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006133:Growth Substances / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000166:cytology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D013696:Temperature
D000952:Antigens, Polyomavirus Transforming; D006133:Growth Substances; D003577:Cytochrome P-450 Enzyme System