Nerve growth factor receptors in the peripubertal rat ovary.
We previously demonstrated that the immature rat ovary synthesizes nerve growth factor (NGF), and that interference of NGF actions by immunoneutralization during neonatal life prevents development of the ovarian sympathetic innervation and delays follicular maturation. Since the actions of NGF are exerted via binding to specific cell surface receptors, the present study was undertaken to define and characterize the presence of NGF receptors (NGFrec) in the developing rat ovary. NGF interacts with two classes of NGFrec. The most abundant is a low affinity form expressed in the central nervous system and peripheral tissues. This receptor is encoded by a single 3.8-kilobase mRNA species. Cross-linking of [125I]NGF to ovarian membranes followed by immunoprecipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and autoradiography showed the presence of a approximately 90-kilodalton molecular species which corresponds in size to the predominant NGF receptor species cross-linked to its ligand. While ovarian NGFrec may be of neuronal origin and reach the gland exclusively by anterograde axonal transport, RNA blot hybridization demonstrated that the ovary expresses the NGFrec mRNA species that encodes the low affinity NGF receptor and, thus, implicated the ovary itself as a site of NGFrec synthesis. NGFrec mRNA levels decreased abruptly after the first ovulation, suggesting that NGFrec may be synthesized in growing follicles and that this capacity is lost after follicular rupture and luteinization.(ABSTRACT TRUNCATED AT 250 WORDS)
Dissen
G A
GA
Division of Neuroscience, Oregon Regional Primate Research Center, Beaverton 97006.
Hill
D F
DF
Costa
M E
ME
Ma
Y J
YJ
Ojeda
S R
SR
D000818:Animals; D001345:Autoradiography; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000378:metabolism*; D010053:Ovary / Q000254:growth & development / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor; D012741:Sexual Maturation; D013799:Theca Cells / Q000166:cytology / Q000502:physiology
D007457:Iodine Radioisotopes; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor