Prolonged period of global ischemia causes no change in the GTP-binding proteins in the isolated perfused rat heart.
The response of the beta-adrenoceptor transduction system to global ischemia for 40 min was investigated in isolated working heart of rat. The enhancement of beta-adrenoceptors was not observed in the ischemic myocardium. A depression of forskolin-stimulated adenylate cyclase enzyme occurred with global ischemia, but no change in Gs or Gi2 was detected. Thus, the present in vitro ischemic heart model may not necessarily reflect the identical milieu induced by the in vivo myocardial ischemia.
Tobise
K
K
First Department of Internal Medicine, Asahikawa Medical College, Japan.
Abe
M
M
Onodera
S
S
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D003327:Coronary Disease / Q000378:metabolism* / Q000503:physiopathology; D019204:GTP-Binding Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
D046911:Macromolecular Substances; D011943:Receptors, Adrenergic, beta; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases