DNA repair characteristics and mutability of the UV-sensitive V79 Chinese hamster cell mutant V-B11 (complementation group 7).
The V79 Chinese hamster cell mutant V-B11 has previously been assigned to a new complementation group (group 7) of UV-sensitive rodent mutants. The D10 for cell survival is approximately 6 J/m2 for V-B11, compared with approximately 15 J/m2 for the parental V79 cell line. The removal of (6-4) photoproducts from the genome overall is not impaired in V-B11, and the level of unscheduled DNA synthesis measured 2 h after UV irradiation is similar to that observed in the parental V79 cells. DNA repair replication measured as a function of UV dose is approximately 50% reduced in V-B11 in comparison with V79, when measured during the first 6 h after UV irradiation. Furthermore, in V-B11 the rate of cyclobutane dimer removal from the HPRT gene is slower than in wild-type cells. Despite the observed defects no effect on the UV-induced frequency of mutants at two loci: Na+/K(+)-ATPase and HPRT was found in V-B11 cells. The properties of V-B11 are compared with those of other UV-sensitive mutants.
Zdzienicka
M Z
MZ
MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratory, The Netherlands.
Mitchell
D L
DL
Venema
J
J
van Hoffen
A
A
van Zeeland
A A
AA
Mullenders
L H
LH
de Wit
J
J
Simons
J W
JW
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D005347:Fibroblasts / Q000528:radiation effects*; D005816:Genetic Complementation Test; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D016296:Mutagenesis; D011740:Pyrimidine Dimers / Q000378:metabolism; D011836:Radiation Tolerance / Q000235:genetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D004247:DNA; D007041:Hypoxanthine Phosphoribosyltransferase; D000254:Sodium-Potassium-Exchanging ATPase