title: DNA replication in Escherichia coli gyrB(Ts) mutants analysed by flow cytometry.
abstract: We have investigated the initiation of DNA replication in Escherichia coli gyrB (Ts) mutants and find that initiations in single cells, even those that occur at non-permissive temperature, are synchronous. Furthermore, our results indicate that the gradual arrest of DNA replication at non-permissive temperature reflects a general decrease in transcription activity and not an initiation-specific function of the DNA gyrase. At an intermediate temperature, the only effect observed was a lack of segregation (decatenation) of replicated chromosomes in a sizeable fraction of the cell population.
authors.affiliation: University Institute of Microbiology, Copenhagen.
authors.forename: U
authors.initials: U
authors.lastname: von Freiesleben
authors.forename: K V
authors.initials: KV
authors.lastname: Rasmussen
mesh_terms: D002453:Cell Cycle; D004261:DNA Replication / Q000235:genetics*; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D005434:Flow Cytometry; D009154:Mutation; D013696:Temperature
chemical_list: D004250:DNA Topoisomerases, Type II