The mitochondrial genome of Schizosaccharomyces pombe. Stimulation of intra-chromosomal recombination in Escherichia coli by the gene product of the first cox1 intron.
The open reading frame of the first intron of the mitochondrial cox1 gene (cox1I1) was expressed in Escherichia coli. The putative intron-encoded protein stimulated the formation of intra-chromosomal lac(+)-recombinants about threefold. No stimulation was found when the reading frame was inserted in the opposite direction, or when it was interrupted by a deletion. The intronic open reading frame did not complement recA- or recB- mutants of E. coli. In S. pombe, elimination of this intron did not abolish homologous recombination in mitochondria. A possible role of the recombinase activity in yeast mitochondria will be discussed.
Manna
F
F
Istituto Internazionale di Genetica e Biofisica, CNR, Napoli, Italy.
Massardo
D R
DR
Del Giudice
L
L
Buonocore
A
A
Nappo
A G
AG
Alifano
P
P
Schäfer
B
B
Wolf
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D004722:Endoribonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D019426:Integrases; D007438:Introns; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D016366:Open Reading Frames; D012326:RNA Splicing; D045522:Recombinases; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D045522:Recombinases; D003576:Electron Transport Complex IV; D004254:DNA Nucleotidyltransferases; D019426:Integrases; D009713:Nucleotidyltransferases; C115006:integron integrase IntI1; C034038:mRNA maturase; D004722:Endoribonucleases