Suitability of different silver enhancement methods applied to 1 nm colloidal gold particles: an immunoelectron microscopic study.
In order to exploit the recently introduced 1 nm gold colloids in routine electron microscopic labeling experiments, an efficient enhancement step for a better visualization of this small marker is a prerequisite. Efficiency and reproducibility of enhancement as well as growth homogeneity of gold particles were evaluated for three different silver intensifying solutions: silver lactate/hydroquinone/gum arabic (Danscher, 1981), Ilford L4/Metol (Bienz et al., 1986), and the commercially available IntenSE M silver enhancement kit (Janssen Pharmaceutica). The best results were obtained by using the silver lactate/hydroquinone/gum arabic mixture. The quality of enhancement of the IntenSE M kit was considerably increased by the addition of the protective colloid gum arabic.
Stierhof
Y D
YD
Max-Planck-Institut für Biologie, Tübingen, Federal Republic of Germany.
Humbel
B M
BM
Schwarz
H
H
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D004926:Escherichia coli / Q000648:ultrastructure*; D006048:Gold Colloid, Radioactive; D006170:Gum Arabic; D006873:Hydroquinones; D007773:Lactates; D019344:Lactic Acid; D016253:Microscopy, Immunoelectron; D012834:Silver
D001425:Bacterial Outer Membrane Proteins; D006048:Gold Colloid, Radioactive; D006873:Hydroquinones; D007773:Lactates; D019344:Lactic Acid; D012834:Silver; D006170:Gum Arabic; C031927:hydroquinone