Mouse platelet-derived growth factor receptor alpha gene is deleted in W19H and patch mutations on chromosome 5.
The mouse W19H mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor alpha gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W19H/ + heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W19H deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.
Smith
E A
EA
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
Seldin
M F
MF
Martinez
L
L
Watson
M L
ML
Choudhury
G G
GG
Lalley
P A
PA
Pierce
J
J
Aaronson
S
S
Barker
J
J
Naylor
S L
SL
D000818:Animals; D015139:Blotting, Southern; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D008040:Genetic Linkage; D006822:Hybrid Cells / Q000166:cytology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008817:Mice, Mutant Strains / Q000235:genetics*; D009115:Muridae; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010982:Platelet-Derived Growth Factor / Q000502:physiology; D011956:Receptors, Cell Surface / Q000235:genetics*; D017479:Receptors, Platelet-Derived Growth Factor; D011995:Recombination, Genetic
D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D004247:DNA; D017479:Receptors, Platelet-Derived Growth Factor