In vivo release of 15-HETE and other arachidonic acid metabolites in nasal secretions during early allergic reactions.
The purpose of this study is to examine the "in vivo" release of 15-HETE and other arachidonic acid metabolites in nasal secretions following a challenge with "Dermatophagoides Pteronyssinus" in patients with allergic rhinitis and non-allergic controls. In addition, we examine the effects of a membrane stabilizer, such as sodium cromoglycate, on these metabolites. Thirteen allergic subjects and seven healthy controls are studied. 15-HETE, peptide leukotrienes, LTB4, PGD2, PGE2 and PGF2 alpha levels are evaluated before and after nasal challenge in sodium cromoglycate treated and untreated subjects. This study provides "in vivo" evidence that the pathophysiological responses to nasal antigen challenge could be related to the release of 15-HETE as well as other arachidonic acid metabolites, mainly arising from the lipoxygenase pathway.
Ramis
I
I
Department of Neurochemistry (Eicosanoid Branch), Centro de Investigacion y Desarrollo, CSIC, Barcelona, Spain.
Catafau
J R
JR
Serra
J
J
Bulbena
O
O
Picado
C
C
Gelpi
E
E
D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D016718:Arachidonic Acid / Q000378:metabolism*; D004205:Cromolyn Sodium / Q000494:pharmacology; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D005260:Female; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism*; D007975:Leukotriene B4 / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism*; D009299:Nasal Provocation Tests; D015230:Prostaglandin D2 / Q000378:metabolism; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy / Q000378:metabolism*
D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D016718:Arachidonic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D015237:Dinoprost; D008084:Lipoxygenase; D015232:Dinoprostone; D004205:Cromolyn Sodium; D015230:Prostaglandin D2