The hygromycin B-resistance-encoding gene as a selectable marker for stable transformation of Trypanosoma brucei.
The hygromycin B (Hy) phosphotransferase-encoding gene (hph), was tested as a selectable marker in the protozoan, Trypanosoma brucei. The hph gene was placed under the control of the promoter of a procyclic acidic repetitive protein-encoding gene, and was integrated by homologous recombination into an intergenic region of the alpha beta-tubulin-encoding gene tandem array of T. brucei. In contrast to many other selectable markers tested, spontaneous Hy resistance was not observed, making Hy a second useful marker for transformation of this protozoan.
Lee
M G
MG
Division of Tropical Medicine, School of Public Health, Columbia University, New York, NY 10032.
van der Ploeg
L H
LH
D000818:Animals; D015139:Blotting, Southern; D004351:Drug Resistance / Q000235:genetics; D005819:Genetic Markers; D006921:Hygromycin B / Q000494:pharmacology*; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D015183:Restriction Mapping; D014170:Transformation, Genetic; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics*
D005819:Genetic Markers; D006921:Hygromycin B; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase