title: Use of protamine sulphate for elimination of substrate DNA in poliovaccines produced on continuous cell lines.
abstract: Cell substrate DNA was shown to be an abundant contaminant in the clarified preparations of the Sabin type 1, 2 and 3 poliovaccines produced on a continuous cell line (4647). The size of the DNA, as evaluated for the Sabin type 1 poliovaccine, was highly heterogeneous, ranging from 100 to 20,000 base pairs. In view of potential oncogenicity of this DNA a simple and efficient procedure for its elimination is proposed. The method is based on use of protamine sulphate which at the concentration of 2.0 mg ml-1 precipitated cell DNA almost completely without affecting the virus titres.
authors.affiliation: Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, Moscow.
authors.forename: F A
authors.initials: FA
authors.lastname: Amosenko
authors.forename: Y V
authors.initials: YV
authors.lastname: Svitkin
authors.forename: V D
authors.initials: VD
authors.lastname: Popova
authors.forename: E N
authors.initials: EN
authors.lastname: Terletskaya
authors.forename: A V
authors.initials: AV
authors.lastname: Timofeev
authors.forename: L B
authors.initials: LB
authors.lastname: Elbert
authors.forename: V A
authors.initials: VA
authors.lastname: Lashkevich
authors.forename: S G
authors.initials: SG
authors.lastname: Drozdov
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA; D017955:Poliovirus / Q000254:growth & development*; D011055:Poliovirus Vaccine, Oral / Q000302:isolation & purification*; D011479:Protamines
chemical_list: D011055:Poliovirus Vaccine, Oral; D011479:Protamines; D004247:DNA