title: The MCK enhancer contains a p53 responsive element.
abstract: p53 is an antioncogene that is defective or absent in a large number of human tumors. Its function in normal cells is not known. We show that co-transfection of mouse p53 with muscle-specific creatine kinase-chloramphenicol acetyltransferase reporter gene, containing 3.3 kilobase of upstream control sequence for the muscle-specific creatine kinase gene, results in a 10- to 80-fold activation. The p53 responsive element maps to a region distinguished from the known MyoD binding region. Identification of a p53 responsive element should allow a more focused analysis of the effects of p53 in controlling gene activity.
authors.affiliation: Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
authors.forename: H
authors.initials: H
authors.lastname: Weintraub
authors.forename: S
authors.initials: S
authors.lastname: Hauschka
authors.forename: S J
authors.initials: SJ
authors.lastname: Tapscott
mesh_terms: D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D003402:Creatine Kinase / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005822:Genetic Vectors; D006801:Humans; D007527:Isoenzymes; D051379:Mice; D009124:Muscle Proteins / Q000235:genetics; D009132:Muscles / Q000201:enzymology; D017570:MyoD Protein; D010957:Plasmids; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D007527:Isoenzymes; D009124:Muscle Proteins; D017570:MyoD Protein; D016159:Tumor Suppressor Protein p53; D015500:Chloramphenicol O-Acetyltransferase; D003402:Creatine Kinase