Genes encoding the platelet-derived growth factor (PDGF) receptor and colony-stimulating factor 1 (CSF-1) receptor are physically associated in mice as in humans.
The BALB/c mouse DNA was analyzed by field-inversion gel electrophoresis to determine the orientation and distance between the beta-platelet-derived growth factor receptor-encoding gene (Pdgfr) and the colony-stimulating factor 1 receptor-encoding gene (Csfmr). It was found that the 5' portion of the Pdgfr gene was cleaved by the enzyme ClaI into two fragments. The 425-kb fragment hybridized with a 3' Pdgfr and a 5' Csfmr probe. This result shows that the Csfmr gene is 3' relative to the Pdgfr gene, and suggests that the Pdgfr and Csfmr genes are physically linked.
Eccles
M R
MR
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
D000818:Animals; D015139:Blotting, Southern; D002895:Chromosomes, Human, Pair 5; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics*; D016184:Receptors, Colony-Stimulating Factor / Q000235:genetics*; D017479:Receptors, Platelet-Derived Growth Factor; D014407:Tumor Cells, Cultured
D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D016184:Receptors, Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D017479:Receptors, Platelet-Derived Growth Factor