title: Unique modification of adenine in genomic DNA of the marine cyanobacterium Trichodesmium sp. strain NIBB 1067.
abstract: The genomic DNA of the marine nonheterocystous nitrogen-fixing cyanobacterium Trichodesmium sp. strain NIBB 1067 was found to be highly resistant to DNA restriction endonucleases. The DNA was digested extensively by the restriction enzyme DpnI, which requires adenine methylation for activity. The DNA composition, determined by high-performance liquid chromatography (HPLC), was found to be 69% AT. Surprisingly, it was found that a modified adenine which was not methylated at the usual N6 position was present and made up 4.7 mol% of the nucleosides in Trichodesmium DNA (15 mol% of deoxyadenosine). In order for adenine residues to be modified at this many positions, there must be many modifying enzymes or at least one of the modifying enzymes must have a degenerate recognition site. The reason(s) for this extensive methylation has not yet been determined but may have implications for the ecological success of this microorganism in nature.
authors.affiliation: Marine Sciences Research Center, State University of New York, Stony Brook 11794.
authors.forename: J P
authors.initials: JP
authors.lastname: Zehr
authors.forename: K
authors.initials: K
authors.lastname: Ohki
authors.forename: Y
authors.initials: Y
authors.lastname: Fujita
authors.forename: D
authors.initials: D
authors.lastname: Landry
mesh_terms: D000225:Adenine / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism*; D008745:Methylation
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D000225:Adenine