IGF-2 receptors are first expressed at the 2-cell stage of mouse development.
A specific IGF-2 receptor antiserum was used to reveal the presence of IGF-2 receptors during preimplantation development of mice. Receptors were present on 2-, 4- and 8-cell embryos, morulae, blastocysts, and on ICMs isolated prior to staining. There was no evidence for receptors on fertilized eggs. These observations confirm reports of the expression of IGF-2 receptor mRNA as early as the 2-cell stage and refine similar observations in blastocysts to confirm expression in both the TE and ICM. A potential auto/paracrine loop is thus one of the first products of activation of the embryonic genome and is expressed constitutively through preimplantation development.
Harvey
M B
MB
Department of Physiology and Pharmacology, University of Queensland, Australia.
Kaye
P L
PL
D000818:Animals; D000911:Antibodies, Monoclonal; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D007150:Immunohistochemistry; D007335:Insulin-Like Growth Factor II / Q000502:physiology*; D051379:Mice; D008856:Microscopy, Fluorescence; D011956:Receptors, Cell Surface / Q000276:immunology / Q000502:physiology*; D017451:Receptors, Somatomedin
D000911:Antibodies, Monoclonal; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D007335:Insulin-Like Growth Factor II