Native yeast telomeres are sufficient to stabilize linear DNA in Xenopus laevis oocytes.
We have constructed a linear plasmid in yeast containing the entire bovine papillomavirus genome and tested its physical stability following microinjection into stage VI oocytes of Xenopus laevis. Our results show that unmodified telomeres, in contrast to the yeast-passaged telomeres, drastically affect the stability of the injected linear plasmid. Plasmids carrying unmodified Tetrahymena thermophila telomeric sequences are rapidly degraded in oocytes. When these plasmids are passed through yeast, the telomere ends become modified by the addition of yeast telomeric sequences. These plasmids are stably maintained in X. laevis oocytes, demonstrating that yeast-modified telomeres are sufficient to prevent linear DNA degradation.
Schmid
M
M
Medizinisch-Naturwissenschaftliches Forschungszentrum Universität Tübingen, F.R.G.
Steinbeisser
H
H
Ascenzioni
F
F
Trendelenburg
M F
MF
Lipps
H J
HJ
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005800:Genes, Fungal; D005814:Genes, Viral; D008845:Microinjections; D009690:Nucleic Acid Conformation; D009865:Oocytes; D010957:Plasmids; D015183:Restriction Mapping; D016615:Telomere; D013698:Templates, Genetic; D016808:Tetrahymena thermophila / Q000235:genetics; D014982:Xenopus laevis
D004247:DNA