title: [Examining the activity of dihydrofolate reduction obtained from beef liver].
abstract: The average values of Vm and Michelis constant Km were determined for enzyme isolated from liver (12.23 mumole/min and 3.2 x 10(-6) mole/dm3. respectively). The enzyme was stable over four months at -20 degrees C. Dissociation constant enzyme-inhibitor (methotrexate) complex Ki is 3.7 x 10(-8) mole/dm3. The reaction rate was sensitive to temperature (5 degrees C increase doubled maximum reaction rate).
authors.affiliation: Katedra i Zakład Chemii Fizycznej, Akademia Medyczna, Poznań.
authors.forename: L
authors.initials: L
authors.lastname: Skibińska
mesh_terms: D000818:Animals; D002417:Cattle; D008099:Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D013696:Temperature; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D013762:Tetrahydrofolate Dehydrogenase
vernacular_title: Badanie aktywności reduktazy dihydrofolianowej otrzymanej z watroby wołowej.