title: The class 3 outer membrane protein (PorB) of Neisseria meningitidis: gene sequence and homology to the gonococcal porin PIA.
abstract: The class 3 protein (PorB) is an important component of the meningococcal outer membrane. The structural gene (porB) encoding the class 3 protein has been cloned using primers suitable for the amplification of the corresponding chromosomal fragment by the polymerase chain reaction (PCR). The complete nucleotide sequence was determined and predicts a mature protein of 310 amino acids, preceded by a signal peptide of 19 residues. The predicted protein sequence of the class 3 protein exhibits essential structural homology to the gonococcal porin PIA. The class 3 protein encoding gene was expressed in Escherichia coli under the control of an inducible promoter.
authors.affiliation: Department of Genetics, Boehringer Mannheim GmbH, Penzberg, F.R.G.
authors.forename: K
authors.initials: K
authors.lastname: Wolff
authors.forename: A
authors.initials: A
authors.lastname: Stern
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D009345:Neisseria meningitidis / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D018272:Porins; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001425:Bacterial Outer Membrane Proteins; D009838:Oligodeoxyribonucleotides; D018272:Porins; C057699:porin protein, Neisseria