The c-mos gene product is required for cyclin B accumulation during meiosis of mouse eggs.
The c-mos protooncogene is expressed as a maternal message that is required for oocyte meiosis. The mechanism of action of c-mos was investigated by analysis of mouse eggs injected with antisense c-mos oligonucleotides. In antisense-injected eggs, maturation-promoting factor was not reactivated after the completion of meiosis I, leading to the formation of nuclei containing decondensed chromatin within 2-3 hr after polar body extrusion. This was correlated with a failure of the injected eggs to accumulate newly synthesized cyclin B. The c-mos protooncogene thus appears to affect the pathway that regulates cyclin B stability during meiosis of mouse eggs.
O'Keefe
S J
SJ
Dana-Farber Cancer Institute, Boston, MA.
Kiessling
A A
AA
Cooper
G M
GM
D000818:Animals; D002478:Cells, Cultured; D016213:Cyclins / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008540:Meiosis; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D006656:Protamine Kinase / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D017231:Proto-Oncogene Proteins c-mos; D011519:Proto-Oncogenes
D016213:Cyclins; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D006656:Protamine Kinase; D017231:Proto-Oncogene Proteins c-mos