[Obtaining monoclonal antibodies to paired tangled filaments from the brain of patients with Alzheimer's disease].
Hybridoma technique was used to obtain a clone producing IgG antibodies. Paired helical neurofilaments isolated from the brain of patients suffering from Alzheimer's disease were used as an antigen during preparation of the clone. Antibodies recognize neurons with neurofibrillary changes. Specificity of the obtained antibodies to paired helical filaments was proved immunohistochemically. Monoclonal antibodies obtained are likely to be useful in the determination of the role played by pathological proteins in mutually twisted filaments formation.
Chumakova
M M
MM
Vostrikov
V M
VM
Dergunova
N N
NN
Rogaeva
E A
EA
D000544:Alzheimer Disease / Q000276:immunology / Q000473:pathology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002540:Cerebral Cortex / Q000276:immunology / Q000648:ultrastructure*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000473:pathology; D016874:Neurofibrillary Tangles / Q000276:immunology*; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G
Poluchenie monoklonal'nykh antitel k poparno skuchennym filamentam iz mozga bol'nykh bolezn'iu Al'tsgeĭmera.