Induction of chromosome abnormalities in mouse and human epidermal keratinocytes by the human papillomavirus type 16 E7 oncogene.
Cytogenetic abnormalities associated with human papillomavirus (HPV) type 16 were studied using primary human and mouse epidermal keratinocytes. The E7 transforming gene of HPV-16 was found to induce chromosome duplication in epidermal keratinocytes; little or no detectable chromosome disorganization was associated with the function of the E6 gene. These results suggest that the E7 gene-linked cytogenetic effect reflects HPV-16-associated pathogenicity in the early phase of transformation.
Hashida
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Laboratory of Molecular and Cell Biology, Kanagawa Cancer Center Research Institute, Yokohama, Japan.
Yasumoto
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D000782:Aneuploidy; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003001:Cloning, Molecular; D004171:Diploidy; D000078404:Epidermal Cells; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D006801:Humans; D007621:Karyotyping; D015603:Keratinocytes / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics* / Q000472:pathogenicity; D050725:Papillomavirus E7 Proteins; D010957:Plasmids; D011123:Polyploidy; D012367:RNA, Viral / Q000032:analysis; D012097:Repressor Proteins; D014162:Transfection
C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012367:RNA, Viral; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16