Spin-trapping studies of hepatic free radicals formed following the acute administration of ethanol to rats: in vivo detection of 1-hydroxyethyl radicals with PBN.
The generation of free radicals in rat liver following the acute oral administration of ethanol was studied with the spin-trapping method, using a deuterated derivative of phenyl-N-tert-butylnitrone (PBN-d14) as the spin-trapping agent. After administration of ethanol and PBN-d14 to rats, organic extracts of the liver were prepared and subjected to ESR spectroscopy. In the case of ethanol-treated rats, the ESR spectra indicated that mixtures of radicals had been trapped, while spectra from control rats were essentially negative. The predominant spin adduct detected after ethanol treatment is proposed to be from a carbon-centered, primary alkyl radical, based on gamma-hydrogen hyperfine splitting patterns observed with PBN-d14. Oxygen-centered radicals also contributed to the ESR spectra. Liver extracts also contained low concentrations of the 1-hydroxyethyl radical spin adduct, which was indicated by weak spectral lines corresponding to those of the 1-13C-ethanol adduct. These data confirm previous suggestions that ethanol is metabolized to a free radical metabolite in rat liver. In addition, some information on types of lipid radicals generated during alcohol intoxication has been obtained.
Reinke
L A
LA
Department of Pharmacology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Kotake
Y
Y
McCay
P B
PB
Janzen
E G
EG
D000435:Alcoholic Intoxication / Q000378:metabolism*; D000818:Animals; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006878:Hydroxides / Q000737:chemistry*; D017665:Hydroxyl Radical; D008099:Liver / Q000737:chemistry* / Q000187:drug effects; D008297:Male; D009589:Nitrogen Oxides; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005609:Free Radicals; D006878:Hydroxides; D009589:Nitrogen Oxides; D013113:Spin Labels; D017665:Hydroxyl Radical; C029217:phenyl-N-tert-butylnitrone; D010100:Oxygen