Immortalization of normal human oral keratinocytes with type 16 human papillomavirus.
Primary human oral keratinocytes were transformed by transfection with recombinant human papillomavirus type 16 (HPV-16) DNA, and two transformed cell lines named human oral keratinocytes-16A and -16B (HOK-16A and HOK-16B) were established. While normal cells and cells transfected with vector only exhibited a limited lifespan, the HOK-16A and HOK-16B lines demonstrated immortality and altered morphology from their normal counterpart. The HOK-16A and HOK-16B lines contained approximately 40 and approximately 25 copies of intact HPV-16 DNA as integrated form per cell respectively, and both cell lines expressed several viral specific poly(A+) RNAs. Notably these cell lines also overexpressed cellular myc proto-oncogene in comparison with the normal counterpart. However, the immortalized cell lines were not able to produce tumors in nude mice, indicating that the cells are partially transformed. The HOK-16A and HOK-16B lines are, therefore, useful for investigating the multistep molecular events of oral carcinogenesis.
Park
N H
NH
UCLA School of Dentistry 90024.
Min
B M
BM
Li
S L
SL
Huang
M Z
MZ
Cherick
H M
HM
Doniger
J
J
D015152:Blotting, Northern; D015139:Blotting, Southern; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D015603:Keratinocytes / Q000473:pathology*; D009061:Mouth Mucosa / Q000473:pathology*; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D010957:Plasmids; D000090063:Proto-Oncogene Mas; D012367:RNA, Viral / Q000235:genetics
D004279:DNA, Viral; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012367:RNA, Viral