[Glycoprotein (gp-51) antigen not associated with the bovine leukemia virus in FLK culture supernatant liquid].
After elimination of the virus fraction non-associated with bovine leukemia virus (free) glycoprotein gp-51 antigen has been preparatively isolated from the supernatant of the FLK cell culture. For this purpose ultrafiltration through PM-30 membrane, thrice-repeated isoelectric focusing with different (pH 3.0-10.0, pH 4.0-6.0) ampholine intervals and affinity chromatography with ConA-sepharose are used. The SDS-polyacrylamide gel electrophoresis has determined it to be a homogeneous protein with molecular weight 51,000 daltons. The results of the enzyme-linked immunosorbent assay, immunoelectrophoresis and agar-gel immunodiffusion test confirm the specific activity of isolated gp-51 antigen. The gp-51 antigen may be used for identification of antibodies in the blood serum of leukemia virus of infected cattle and for monoclonal screening of antibodies.
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D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000032:analysis*; D012756:Sheep
D000956:Antigens, Viral; D008562:Membrane Glycoproteins
Neassotsiirovannyĭ s virusom leĭkoza krupnogo rogatogo skota glikoproteidnyĭ (gp-51) antigen v supernatante kul'tury FLK-kletok.