Analytical and micropreparative capillary electrophoresis of the peptides from calcitonin.
The capillary electrophoresis (CE) of peptide fragments from the tryptic digest of salmon calcitonin and elcatonin has been carried out in H2O- and D2O-based buffer solutions. Analysis in heavy water was found to be superior to that in H2O especially at a pH or pD of 7.93. From a single CE run on elcatonin digest we were also able to harvest three pure cleavage peptides in sufficient quantity to determine each amino acid residue by protein sequencing. The order of elution from CE agreed with that predicted on the basis of net charge calculated for each peptide.
Camilleri
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P
SmithKline Beecham Pharmaceuticals, Welwyn, Herts, United Kingdom.
Okafo
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GN
Southan
C
C
Brown
R
R
D000595:Amino Acid Sequence; D000818:Animals; D002116:Calcitonin / Q000031:analogs & derivatives / Q000737:chemistry*; D003903:Deuterium / Q000378:metabolism; D017666:Deuterium Oxide; D004586:Electrophoresis / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D012474:Salmon; D014357:Trypsin / Q000378:metabolism; D014867:Water / Q000378:metabolism
D010455:Peptides; D014867:Water; D002116:Calcitonin; D003903:Deuterium; D014357:Trypsin; D017666:Deuterium Oxide; C012755:elcatonin