title: Site-specific methylation of 16S rRNA caused by pct, a pactamycin resistance determinant from the producing organism, Streptomyces pactum.
abstract: Ribosomal resistance to pactamycin in clones of Streptomyces lividans containing DNA (pct) from Streptomyces pactum, the pactamycin producer, involves methylation of 16S RNA. The modified residue A-941 in S. lividans 16S rRNA (A-964 in the homologous Escherichia coli sequence) is converted to 1-methyladenosine, and the ribosomal ability to bind pactamycin is reduced or abolished.
authors.affiliation: Centro de Biologia Molecular, Consejo Superior de Investigaciones Científicas, Canto Blanco, Madrid, Spain.
authors.forename: J P
authors.initials: JP
authors.lastname: Ballesta
authors.forename: E
authors.initials: E
authors.lastname: Cundliffe
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D004352:Drug Resistance, Microbial / Q000235:genetics*; D008745:Methylation; D008961:Models, Structural; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010142:Pactamycin / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification / Q000378:metabolism*; D016914:Ribonuclease H; D012270:Ribosomes / Q000201:enzymology; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010142:Pactamycin; D016914:Ribonuclease H