title: In vitro production of antimitochondrial antibody by peripheral blood mononuclear cells in patients with primary biliary cirrhosis.
abstract: Peripheral blood mononuclear cells from patients with primary biliary cirrhosis (PBC) were studied for their ability to produce antibodies to mitochondrial antigens (antimitochondrial antibody, AMA and anti-pyruvate dehydrogenase complex, anti-PDC), using dot-immunobinding and ELISA assay. 2 x 10(6) B cells alone and the mixture of 1.4 x 10(6) T plus 0.6 x 10(6) B cells from patients with serum AMA+ PBC spontaneously produced similar amounts of AMA and anti-PDC. Coculture experiments of T and B cells showed that anti-PDC production was significantly greater in pokeweed mitogen (PWM)-stimulated cultures than in unstimulated ones. The reactivities of antibodies to mitochondrial antigens were inhibited by M2 mitochondrial antigen-enriched fraction. Ursodeoxycholic acid added to cultures resulted in a significant inhibition for production of immunoglobulins and anti-PDC (IgG and IgM). Spontaneous production of AMA or anti-PDC was not detected in patients with AMA- PBC, chronic viral hepatitis and healthy controls, nor could it be induced by PWM in either group. EBV-transformed B cell line established from a AMA+ PBC patient produced high levels of AMA and IgM class anti-PDC. These findings indicate that antibody to a specific mitochondrial antigen in patients with PBC may be produced in T cell-dependent and-independent manner.
authors.affiliation: Third Department of Internal Medicine, Nagoya University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishikawa
authors.forename: S
authors.initials: S
authors.lastname: Kakumu
authors.forename: T
authors.initials: T
authors.lastname: Wakita
authors.forename: M
authors.initials: M
authors.lastname: Takayanagi
authors.forename: K
authors.initials: K
authors.lastname: Yoshioka
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007118:Immunoassay; D066298:In Vitro Techniques; D008105:Liver Cirrhosis, Biliary / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000276:immunology*
chemical_list: D001323:Autoantibodies