Effect of acute administration of gliclazide on the glucose sensitivity of pancreatic B-cells in healthy subjects.
1. Sulphonylurea derivatives are commonly used in the treatment of non-insulin-dependent diabetes mellitus. It is, however, unclear whether the hypoglycaemic effect of sulphonylurea derivatives is additive to the effect of glucose, or whether sulphonylurea derivatives act by increasing B-cell glucose sensitivity. 2. We assessed the effect of gliclazide on glucose-stimulated insulin secretion in eight healthy volunteers. Sixty minute hyperglycaemic glucose clamps (blood glucose levels of 8, 11 and 32 mmol/l) were performed, with and without prior administration of gliclazide (80 mg) 90 min before the glucose clamp. 3. Dose-response characteristics were assessed with a modified Michaelis-Menten equation. The Vmax. (maximal B-cell responsiveness) was not significantly changed (1.5 +/- 0.1 versus 1.3 +/- 0.2 and 5.0 +/- 0.5 versus 4.8 +/- 0.5 mmol/l for the first- and second-phase insulin secretion, respectively), whereas the ED50 (half-maximally stimulating blood glucose concentration) was significantly decreased by gliclazide for first-phase insulin secretion (7.6 +/- 0.3 versus 9.1 +/- 0.6 mmol/l) but not for second-phase insulin secretion (12.0 +/- 0.5 versus 12.3 +/- 0.5 mmol/l). 4. We conclude that gliclazide indeed leads to a shift to the left of the dose-response curve of first-phase insulin release in vivo without a change in Vmax, which indicates an apparent enhancement of B-cell glucose sensitivity.
Veneman
T F
TF
Department of Endocrinology, Free University Hospital, Amsterdam, The Netherlands.
van Haeften
T W
TW
van der Veen
E A
EA
D000328:Adult; D004305:Dose-Response Relationship, Drug; D005907:Gliclazide / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D015309:Glucose Clamp Technique; D006801:Humans; D007328:Insulin / Q000096:biosynthesis*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*
D007328:Insulin; D005907:Gliclazide; D005947:Glucose