Hepatocyte growth factor modulates migration and proliferation of human microvascular endothelial cells in culture.
Epidermal growth factor (EGF) induces tubular formation of cultured human omental microvascular endothelial (HOME) cells and EGF also stimulates cell migration as well as expression of tissue type plasminogen activator (t-PA). Here we studied the effects of hepatocyte growth factor (HGF) on cell proliferation, cell migration and expression of t-PA and other related genes. Migration of confluent HOME cells into the denuded space was stimulated by HGF after being wounded with razor blade, but at a reduced rate in comparison with EGF. HOME cells could be proliferated in response to exogenous 100 ng/ml of HGF at rates comparable to that of 20 ng/ml EGF. The chemotactic activity of HOME cells was significantly stimulated by HGF in a dose-dependent manner when assayed by Boyden chamber. HGF did not efficiently enhance expression of both the t-PA gene and a tissue inhibitor of metalloproteinase gene whereas it stimulated expression of plasminogen activator inhibitor-1. Our present study provides a new evidence that some of the biological effects of HGF on HOME cells in culture are similar to those of EGF.
Morimoto
A
A
Department of Biochemistry, Oita Medical School, Japan.
Okamura
K
K
Hamanaka
R
R
Sato
Y
Y
Shima
N
N
Higashio
K
K
Kuwano
M
M
D001663:Bilirubin / Q000494:pharmacology*; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D015342:DNA Probes; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D008666:Metalloendopeptidases / Q000378:metabolism; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors; D009852:Omentum; D015849:Plasminogen Inactivators / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012709:Serum Albumin / Q000494:pharmacology*; D000075462:Serum Albumin, Human; D019714:Tissue Inhibitor of Metalloproteinases; D010959:Tissue Plasminogen Activator / Q000378:metabolism
D015342:DNA Probes; D006023:Glycoproteins; D006133:Growth Substances; D015849:Plasminogen Inactivators; D012333:RNA, Messenger; D012709:Serum Albumin; D019714:Tissue Inhibitor of Metalloproteinases; C053498:albumin-bilirubin complex; D004815:Epidermal Growth Factor; D010959:Tissue Plasminogen Activator; D008666:Metalloendopeptidases; D003012:Microbial Collagenase; D001663:Bilirubin; D000075462:Serum Albumin, Human