Idiotypic analysis of human anticentromere autoantibodies.
The idiotypes (Ids) of anticentromere antibodies (ACA) have been studied using a fusion protein obtained from cloned cDNA of the major centromere antigen, CENP-B, for isolation of the autoantibodies. IgG-ACA were affinity purified from 4 patient sera and anti-Ids prepared in rabbits. Analysis revealed the existence of two distinct types of immunodominant Ids. One Id is near the antibody combining site and one is framework associated. A longterm longitudinal study of Id expression in a patient who seroconverted from ACA (-) to ACA (+) when she developed Raynaud's phenomenon showed a close correlation between Id expression and ACA titers (r = 0.94). These results may be interpreted as evidence for an autoantigen driven process in the anticentromere immune response.
Hildebrandt
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S
Department of Medicine, University of Connecticut School of Medicine, Farmington 06032.
Weiner
E S
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Earnshaw
W C
WC
Zanetti
M
M
Rothfield
N F
NF
D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000276:immunology*; D000918:Antibody Specificity; D001324:Autoantigens; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002503:Centromere / Q000276:immunology*; D051936:Centromere Protein B; D002846:Chromatography, Affinity; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D004268:DNA-Binding Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D008137:Longitudinal Studies; D008875:Middle Aged; D009195:Peroxidase / Q000494:pharmacology; D011928:Raynaud Disease / Q000276:immunology
D000974:Antibodies, Antinuclear; D001324:Autoantigens; C497926:CENPB protein, human; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D009195:Peroxidase