[Participation of gangliosides in protection of beta adrenergic receptors from damaging effect induced by lipid peroxidation in synaptosome membranes].
The induction of lipid peroxidation (LPO) in rat brain synaptosomes was shown to result in considerable decrease of the level of specific [3H]-dihydroalprenolol binding, decrease of Bmax and increase of KD. It was revealed that the preincubation of rat brain synaptosomes with monosialoganglioside GM1 (10(-8) M) or alpha-tocopherol (10(-6) M) led to a decrease in MDA accumulation after LPO induction by Fe(2+)-ascorbate system. AT the same time GM1 prevents damage of beta-adrenoreceptors, caused by LPO induction, having no effect on the functional state of beta-adrenoreceptors in control preparations. Partial normalization of the ligand affinity of the receptors was observed after preincubation of synaptosomes with GM1 and alpha-tocopherol. The various mechanisms of stabilization of synaptosomal membranes by gangliosides and natural antioxidant-tocopherol is suggested.
Tiurin
V A
VA
Kuznetsova
L A
LA
Tiurina
Iu Iu
IuIu
Erin
A N
AN
Avrova
N F
NF
Pertseva
M N
MN
Kagan
V E
VE
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*; D013053:Spectrophotometry; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
D011943:Receptors, Adrenergic, beta; D014810:Vitamin E; D005677:G(M1) Ganglioside; D008315:Malondialdehyde
Uchastie gangliozidov v zashchite beta-adrenoretseptorov ot povrezhdaiushchego vozdeĭstviia, vyzvannogo perekisnym okisleniem lipidov, v membranakh sinaptosom.