title: Growth factor-like effect of urokinase type plasminogen activator in human renal cells.
abstract: Human renal glomerular epithelial cells possess membrane urokinase receptors. Addition of purified active urokinase to these cells in serum free minimum medium induced a dose-dependent increase in 3H-thymidine incorporation and a doubling of cell number after 48 hours of incubation. Both receptor occupancy and enzymatic activity of u-PA were required to stimulate cell proliferation. This effect was inhibited by down regulation of protein kinase C (PKC) or by H7, an inhibitor of PKC. It involved a pertussis toxin-sensitive pathway. This effect of urokinase was additive with EGF but not with thrombin growth factor activity and was not inhibited by aprotinin, an inhibitor of plasmin.
authors.affiliation: INSERM U 64, Hôpital Tenon, Paris, France.
authors.forename: C J
authors.initials: CJ
authors.lastname: He
authors.forename: J M
authors.initials: JM
authors.lastname: Rebibou
authors.forename: M N
authors.initials: MN
authors.lastname: Peraldi
authors.forename: Q
authors.initials: Q
authors.lastname: Meulders
authors.forename: E
authors.initials: E
authors.lastname: Rondeau
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D007678:Kidney Glomerulus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D037342:Pertussis Toxin; D010879:Piperazines / Q000494:pharmacology; D010960:Plasminogen Activators / Q000494:pharmacology*; D015849:Plasminogen Inactivators / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D055293:Receptors, Urokinase Plasminogen Activator; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism / Q000494:pharmacology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D006133:Growth Substances; D007546:Isoquinolines; C524898:PLAUR protein, human; D010879:Piperazines; D015849:Plasminogen Inactivators; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D010566:Virulence Factors, Bordetella; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D037342:Pertussis Toxin; D011493:Protein Kinase C; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine