title: The retrotransposon copia regulates Drosophila gene expression both positively and negatively.
abstract: The D. melanogaster transposable element copia is structurally similar to retroviral proviruses. We have asked whether copia encodes regulatory functions which have been observed in certain other proviruses. We have introduced reporter constructs based on the copia promoter and other Drosophila promoters into Drosophila cells and asked if copia has any affect on their expression. We find that, whilst copia negatively regulates expression from its own promoter, it also positively regulates expression from the larval serum protein 1 promoter. Analysis of RNA suggests that both regulatory functions occur by post-transcriptional mechanisms.
authors.affiliation: Department of Medicine, University of Cambridge, UK.
authors.forename: L A
authors.initials: LA
authors.lastname: Bryant
authors.forename: C
authors.initials: C
authors.lastname: Brierley
authors.forename: A J
authors.initials: AJ
authors.lastname: Flavell
authors.forename: J H
authors.initials: JH
authors.lastname: Sinclair
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics; D005786:Gene Expression Regulation; D007301:Insect Hormones / Q000235:genetics; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D007301:Insect Hormones; D012333:RNA, Messenger; C034648:larval serum protein, Drosophila