title: Altered localization of antigen recognized by proteinuria-inducing monoclonal antibody in experimental nephrosis.
abstract: Change in the localization of the antigen recognized by the proteinuria-inducing monoclonal antibody (MA) 5-1-6 in experimental nephrosis was studied by indirect and biotin-avidin immunofluorescence, and immunoperoxidase at light and electron microscopical levels. The proteinuric state was induced by the administration of the aminonucleoside of puromycin (PAN) or adriamycin. The antigen decreased in quantity and/or its distribution changed with an increase in the amount of protein excreted in both experimental models. Recovery from the alterations observed during the development and proteinuria appeared to occur when PAN-induced proteinuria subsided. This antigenic molecule may thus be essential for maintaining the normal permselectivity of glomerular capillary walls.
authors.affiliation: Department of Immunology, Niigata University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Okasora
authors.forename: M
authors.initials: M
authors.lastname: Nagase
authors.forename: H
authors.initials: H
authors.lastname: Kawachi
authors.forename: K
authors.initials: K
authors.lastname: Matsui
authors.forename: M
authors.initials: M
authors.lastname: Orikasa
authors.forename: T
authors.initials: T
authors.lastname: Morioka
authors.forename: I
authors.initials: I
authors.lastname: Yamazaki
authors.forename: T
authors.initials: T
authors.lastname: Oite
authors.forename: F
authors.initials: F
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology / Q000378:metabolism*; D004317:Doxorubicin; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008297:Male; D016253:Microscopy, Immunoelectron; D009401:Nephrosis / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D011507:Proteinuria / Q000276:immunology* / Q000473:pathology; D011692:Puromycin Aminonucleoside; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D011692:Puromycin Aminonucleoside; D004317:Doxorubicin