Fractionation of cellulases from the ruminal fungus Neocallimastix frontalis EB188.
Cellulases from the ruminal fungus Neocallimastix frontalis EB188 were separated by using hydroxylapatite column chromatography. Seven carboxymethylcellulases, six avicelases, and four beta-glucosidases accounted for the majority of the activities. The separation of enzymes was confirmed by using polyacrylamide gel electrophoresis. Electrophoretic migration, analysis of hydrolysis products, and substrate specificity measurements suggested that several different cellulases were secreted in N. frontalis EB188. The possible relationship of cellulase diversity to protein glycosylation is discussed.
Li
X L
XL
Department of Animal Sciences, Washington State University, Pullman 99164-6332.
Calza
R E
RE
D000818:Animals; D002417:Cattle; D002480:Cellulase / Q000737:chemistry / Q000302:isolation & purification*; D005591:Chemical Fractionation; D002853:Chromatography, Liquid; D008411:Chytridiomycota / Q000737:chemistry / Q000201:enzymology*; D003470:Culture Media; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D006882:Hydroxyapatites; D012417:Rumen / Q000382:microbiology*
D003470:Culture Media; D005656:Fungal Proteins; D006882:Hydroxyapatites; D017886:Durapatite; D002480:Cellulase