[Determination of endoxin in hypertensive patients].
Digoxin like immunoreactive factor (DLIF), has been implicated on the effect of sodium in essential hypertension. The different concentration of DLIF as a function of sodium intake was demonstrated in animal experience by some authors. In this work the urinary DLIF excretion is evaluated by RIA and its biological activity by Na+/K+ ATPase inhibition, in 5 urine samples at the end of a free sodium diet week and in 10 urine samples in the last day of a week with 250 mg sodium diet. The urinary DLIF excretion after the free sodium diet week was 0.3460 +/- 0.055 and at the end of sodium restriction diet week of 0.2910 +/- 0.061 nmol/l. Although the DLIF values in the sodium restriction week were smaller than the DLIF values of the free sodium diet week, there was no statistical difference (p = 0.113). In five patients the DLIF could be evaluated at the end of the first and second weeks without changes in the hypertensive therapeutics, with clonidine and nifedipine, along the two weeks. In these five patients at the end of the free sodium diet week and at end of the sodium restricted diet week were 0.3460 +/- 0.055 and 0.2780 +/- 0.060 nmol/l. The reduction of urinary DLIF excretion in the sodium restricted diet week, was significative (p = 0.020). The results of the Na/K ATPase inhibition in the same five patients were: 34.6 +/- 6.51% at the end of the free sodium diet week and 31.7 +/- 6.32% at the end of the sodium restricted diet week, the differences were not statistically significant.(ABSTRACT TRUNCATED AT 250 WORDS)
Gorjão Clara
J
J
Instituto de Química Fisiológica, Centro de Metabolismo e Endocrinologia (Mb L2).
Breitenfeld
B
B
Bicho
M
M
Moreira
C
C
Carvalho
C
C
Boaventura
I
I
Manso
C
C
Nogueira da Costa
J
J
D000328:Adult; D001798:Blood Proteins / Q000652:urine*; D002298:Cardenolides; D004077:Digoxin; D006801:Humans; D006973:Hypertension / Q000652:urine*; D008875:Middle Aged; D012503:Saponins; D012982:Sodium, Dietary / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; D012982:Sodium, Dietary; C034504:digoxin-like factors; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase
Doseamento da endoxina em hipertensos.