title: The latent membrane protein-1 in Epstein-Barr virus-transformed lymphoblastoid cells is found with ubiquitin-protein conjugates and heat-shock protein 70 in lysosomes oriented around the microtubule organizing centre.
abstract: Immunofluorescence studies on Epstein-Barr virus (EBV)-transformed lymphoblastoid cells have previously shown that the latent membrane transforming protein (LMP-1) is found in patch-like inclusions which also immunostain for vimentin. We now show that EBV transformation causes a major reorganization of intermediate filaments, microtubules, mitochondria, and lysosomal elements, which generally become oriented around the microtubule organizing centre. Immunogold electron microscopy shows that LMP-1 is primarily concentrated in secondary lysosomes together with ubiquitin-protein conjugates and heat-shock protein 70. Intermediate filament inclusion formation with the above characteristics may be a general response triggered by other membrane glycoproteins; as seen, for example, in major human neurodegenerative diseases such as diffuse Lewy body disease.
authors.affiliation: Department of General Zoology, Eötvös University, Budapest, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: László
authors.forename: J
authors.initials: J
authors.lastname: Tuckwell
authors.forename: T
authors.initials: T
authors.lastname: Self
authors.forename: J
authors.initials: J
authors.lastname: Lowe
authors.forename: M
authors.initials: M
authors.lastname: Landon
authors.forename: S
authors.initials: S
authors.lastname: Smith
authors.forename: J N
authors.initials: JN
authors.lastname: Hawthorne
authors.forename: R J
authors.initials: RJ
authors.lastname: Mayer
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D001402:B-Lymphocytes / Q000737:chemistry* / Q000648:ultrastructure; D002461:Cell Line, Transformed; D006360:Heat-Shock Proteins / Q000032:analysis*; D004854:Herpesvirus 4, Human; D006801:Humans; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000648:ultrastructure; D008247:Lysosomes / Q000737:chemistry*; D008854:Microscopy, Electron; D008870:Microtubules / Q000737:chemistry / Q000648:ultrastructure*; D014452:Ubiquitins / Q000032:analysis; D014746:Vimentin / Q000032:analysis; D014759:Viral Envelope Proteins / Q000032:analysis*; D014763:Viral Matrix Proteins
chemical_list: D000956:Antigens, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D006360:Heat-Shock Proteins; D014452:Ubiquitins; D014746:Vimentin; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins