Interaction of RuCl2 (dimethylsulphoxide)4 isomers with DNA.
A wide series of restriction enzymes with a range of specificities was used to investigate the interaction with DNA of Ru(II)-DMSO complexes with anticancer activity. While cis-RuCl2 (DMSO)4 was almost completely inactive, treatment of pBR 322 DNA with trans-RuCl2 (DMSO)4 protected G rich sequences from cutting of specific restriction endonucleases, indicating a preferential interaction with adjacent guanines.
Loseto
F
F
Dip. Scienze Biomediche e Oncologia Umana, Policlinico, Bari, Italy.
Alessio
E
E
Mestroni
G
G
Lacidogna
G
G
Nassi
A
A
Giordano
D
D
Coluccia
M
M
D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000032:analysis*; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D004121:Dimethyl Sulfoxide