Effect of tocotrienols on hepatocarcinogenesis induced by 2-acetylaminofluorene in rats.
The effects of tocotrienols on hepatocarcinogenesis in rats fed with 2-acetylaminofluorene (AAF) were followed morphologically and histologically for a period of 20 wk. No differences between treated and control rats in the morphology and histology of their livers was observed. Cell damage was extensive in the livers of AAF-treated rats but less extensive in the AAF-tocotrienols-treated rats when compared with normal and tocotrienols-treated rats. 2-Acetylaminofluorene significantly increases the activities of both plasma and liver microsomal gamma-glutamyltranspeptidase (GGT) and liver microsomal UDP-glucuronyltransferase (UDP-GT). Tocotrienols administered together with AAF significantly decrease the activities of plasma GGT after 12 and 20 wk (P less than 0.01, P less than 0.002, respectively) and liver microsomal UDP-GT after 20 wk (P less than 0.02) when compared with the controls and with rats treated only with tocotrienols. Liver microsomal GGT also showed a similar pattern to liver microsomal UDP-GT but the decrease was not significant. These results suggest that tocotrienols administered to AAF-treated rats reduce the severity of hepatocarcinogenesis.
Ngah
W Z
WZ
Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur.
Jarien
Z
Z
San
M M
MM
Marzuki
A
A
Top
G M
GM
Shamaan
N A
NA
Kadir
K A
KA
D015073:2-Acetylaminofluorene; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D014453:Glucuronosyltransferase / Q000097:blood; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000517:prevention & control* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000627:therapeutic use; D005723:gamma-Glutamyltransferase / Q000097:blood
D000975:Antioxidants; D014810:Vitamin E; D015073:2-Acetylaminofluorene; D005723:gamma-Glutamyltransferase; D014453:Glucuronosyltransferase