title: [Studies on antiaggregating drugs of horseshoe crab amebocytes].
abstract: The effects of drugs on amebocyte spontaneous aggregation and endotoxin induced aggregation were studied. N-Ethylmaleimide, ethylenediamine tetraacetate, propranolol, verapamil and lidocaine but not dibutyryl cyclic AMP could reduce the aggregating rate of amebocytes. The speed of degranulation induced by endotoxin was reduced by N-ethylmaleimide, ethylenediamine tetraacetate, propranolol, verapamil and caffeine. These results suggest that Ca+2 and c-AMP may play an important role in amebocyte spontaneous aggregation and endotoxin induced aggregation. The blocking agent of Ca+2 and c-AMP can also antagonize the aggregation of amebocytes.
authors.affiliation: Department of Pharmacology, Kaohsiung Medical College, Taiwan, Republic of China.
authors.forename: I J
authors.initials: IJ
authors.lastname: Chen
authors.forename: S J
authors.initials: SJ
authors.lastname: Hwang
authors.forename: Y M
authors.initials: YM
authors.lastname: Chen
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D005260:Female; D008033:Limulus Test; D008297:Male; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
chemical_list: D010975:Platelet Aggregation Inhibitors; D011433:Propranolol; D014700:Verapamil; D000242:Cyclic AMP; D002118:Calcium