Estimation of IgE antibodies to the common allergens by reverse enzyme immunoassay. Comparison with the radioallergosorbent test.
A reverse enzyme immunoassay (REINA) is described, in which polystyrene microtiter wells are sensitized with murine monoclonal anti-human IgE, and then sequentially allowed to react with patient's serum, peroxidase-labeled allergens and substrate. The results obtained with the sera of patients allergic to Lolium perenne grass pollen, the tree pollens of Betula alba and Olea europea, the epithelia of cat and dog, the mite Dermatophagoides pteronyssinus, or to the foodstuffs cow's milk, chicken eggwhite or peanut were compared with the analytical data from the ratio allergosorbent test (RAST). The results show a good correlation between these two laboratory techniques.
Casanovas
M
M
CBF Research Group, Madrid, Spain.
Bel
I
I
Marañón
F
F
Berrens
L
L
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D010367:Arachis / Q000276:immunology; D002415:Cats; D004285:Dogs; D004527:Egg Proteins / Q000276:immunology; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000097:blood*; D051379:Mice; D008892:Milk / Q000276:immunology; D008925:Mites / Q000276:immunology; D011058:Pollen / Q000276:immunology; D011852:Radioallergosorbent Test / Q000379:methods*; D012867:Skin / Q000276:immunology
D000485:Allergens; D000911:Antibodies, Monoclonal; D004527:Egg Proteins; D007073:Immunoglobulin E