Development and testing of a packaging cell line for avian retroviral vectors.
A new helper cell line designated L3.07, has been used to package spleen necrosis virus (SNV)-based vectors, resulting in the production of high titres of replication defective retroviruses. One of these vectors, vSNO21 has been shown to infect avian primordial germ cells (PGCs).
Meyers
N L
NL
AFRC Institute for Animal Health, Houghton Laboratory, Huntingdon, England.
Booth
S C
SC
Bumstead
N
N
Vick
L
L
Simkiss
K
K
D000818:Animals; D002460:Cell Line; D003370:Coturnix; D005347:Fibroblasts; D005822:Genetic Vectors; D051379:Mice; D010957:Plasmids; D015183:Restriction Mapping; D013539:Simian virus 40 / Q000235:genetics* / Q000648:ultrastructure; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication
D015678:Viral Structural Proteins