The cytochrome chain of mitochondria exhibits variable H+/e- stoichiometry.
A study is presented of the ----H+/e- stoichiometry for H+ pumping by the cytochrome chain in isolated rat liver mitochondria under level-flow and steady-state conditions. It is shown that the ----H+/e- stoichiometry for the cytochrome chain varies under the influence of the flow rate and transmembrane delta microH+. The rate-dependence is shown to be associated with cytochrome c oxidase, whose ----H+/e- ratio varies from 0 to 1, whilst the ----H+/e- ratio for the span covered by cytochrome c reductase is invariably 2.
Papa
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S
Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.
Capitanio
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N
Capitanio
G
G
De Nitto
E
E
Minuto
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M
D000818:Animals; D004579:Electron Transport / Q000502:physiology*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D051381:Rats; D013053:Spectrophotometry; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
D013386:Succinates; D006859:Hydrogen; D019802:Succinic Acid; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV