title: GroE facilitates refolding of citrate synthase by suppressing aggregation.
abstract: The molecular chaperone GroE facilitates correct protein folding in vivo and in vitro. The mode of action of GroE was investigated by using refolding of citrate synthase as a model system. In vitro denaturation of this dimeric protein is almost irreversible, since the refolding polypeptide chains aggregate rapidly, as shown directly by a strong, concentration-dependent increase in light scattering. The yields of reactivated citrate synthase were strongly increased upon addition of GroE and MgATP. GroE inhibits aggregation reactions that compete with correct protein folding, as indicated by specific suppression of light scattering. GroEL rapidly forms a complex with unfolded or partially folded citrate synthase molecules. In this complex the refolding protein is protected from aggregation. Addition of GroES and ATP hydrolysis is required to release the polypeptide chain bound to GroEL and to allow further folding to its final, active state.
authors.affiliation: Institut für Biophysik und Physikalische Biochemie, Universität Regensburg, FRG.
authors.forename: J
authors.initials: J
authors.lastname: Buchner
authors.forename: M
authors.initials: M
authors.lastname: Schmidt
authors.forename: M
authors.initials: M
authors.lastname: Fuchs
authors.forename: R
authors.initials: R
authors.lastname: Jaenicke
authors.forename: R
authors.initials: R
authors.lastname: Rudolph
authors.forename: F X
authors.initials: FX
authors.lastname: Schmid
authors.forename: T
authors.initials: T
authors.lastname: Kiefhaber
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D018835:Chaperonin 10; D018834:Chaperonin 60; D018833:Chaperonins; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D046911:Macromolecular Substances; D009206:Myocardium / Q000201:enzymology; D011487:Protein Conformation; D012542:Scattering, Radiation; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; C028715:GroE protein, E coli; D006360:Heat-Shock Proteins; D046911:Macromolecular Substances; D002950:Citrate (si)-Synthase; D018833:Chaperonins