[Heavy water (D2O) as a protective protein containing medium. Example: human cholinesterase].
Looking for a possible protective effect of heavy water on proteins, cholinesterase and lactate dehydrogenase in combination with D2O and further protective substances were exposed to a temperature of 60 degrees C (for 10 min) and about 45 degrees C (for several days). In combination with glycerine there resulted an additive protective effect; with NaCl and/or albumin being added the individual effects raised to a higher level. In the cold D2O protects cholinesterase only against acid denaturation, but in combination with warmth also against basic denaturation.
Heyde
C
C
Biologisch-Chemische Abteilung, Freie Universität Berlin, Bundesrepublik Deutschland.
Wenzel
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M
D002802:Cholinesterases / Q000097:blood / Q000737:chemistry*; D003903:Deuterium; D017666:Deuterium Oxide; D004355:Drug Stability; D004795:Enzyme Stability; D006801:Humans; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry*; D013816:Thermodynamics; D013997:Time Factors; D014867:Water
D007202:Indicators and Reagents; D011506:Proteins; D014867:Water; D003903:Deuterium; D007770:L-Lactate Dehydrogenase; D002802:Cholinesterases; D017666:Deuterium Oxide
Schweres Wasser (D2O) als Protektivum proteinhaltiger Arzneimittel. Beispiel: humane Cholinesterase.