[Function of the InsA gene in the IS1 element of the Tn9' transposon: influence of oligonucleotide inserts in the InsA gene on formation of simple insertions and plasmid cointegrates].
To elucidate the role of the insA reading frame in transposition of the IS1 element of the Tn9' transposon, the derivatives of plasmids pUC19::Tn9' and pUC19::IS1 have been obtained using oligonucleotide inserts of the length equal or exceeding 9 bp and equal to 10 bp. The ability of mutant variants of the Tn9' transposon and the IS1 element to form simple insertions and plasmid cointegrates was studied. To this end, experiments were performed on mobilization of the derivatives of pUC19 containing mutant variants of the IS1 element and Tn9' as well as of the plasmids pUC19::Tn9' by the conjugative plasmid pRP3.1. According to the data obtained, mutations (inserts) in the insA gene have no influence on the frequency of transposition of the IS1 element and Tn9' from the plasmid pUC19 to pRP3.1. At the same time, the frequency of transposition events of mutant variants of Tn9' from the plasmid pRP3.1 to pBR322 is more than 10 times lower in comparison with the wild type transposon. The data obtained are in accordance with the assumption that the insA gene is not essential for transposition. A hypothesis is put forward explaining the role of the insA gene product in the process of bringing together short inverted repeats of the IS1, which are the sites for the transposase to be recognized at first stages of transposition.
Duzhiĭ
D E
DE
Danilevich
V I
VI
Sukhodolets
V V
VV
D004251:DNA Transposable Elements / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics*; D009154:Mutation; D009841:Oligonucleotides / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*
D004251:DNA Transposable Elements; D009841:Oligonucleotides
Izuchenie funktsii gena InsA élementa IS1 v sostave transpozona Tn9': vliianie oligonukleotidnykh vstavok v InsA na obrazovanie prostykh insertsiĭ i plazmidnykh kointegratov.