Two components of use-dependent block of Na+ current by disopyramide and lidocaine in guinea pig ventricular myocytes.
We studied the kinetics of the use-dependent block of the Na+ current (INa) by disopyramide and lidocaine. INa was recorded from isolated guinea pig ventricular myocytes by using the whole-cell patch-clamp technique. The use-dependent block of INa by disopyramide with 20- and 200-msec depolarizing pulses developed in two exponential functions. The degree of the use-dependent block and the amplitude of the fast (Af) and slow (As) components with the short (20-msec) pulse protocol were comparable to those with the long (200-msec) pulse protocol. When pH was raised from 7.3 to 8.0, disopyramide increased Af without a change in As. At pH 6.5, INa block developed with a single exponential function revealing only the slow component. The fast and slow components of INa block by disopyramide could be explained by binding of the uncharged and charged forms, respectively, to the activated state of the channel. Development of INa block by lidocaine also was expressed by two exponentials at all pulse durations (5-200 msec). As pulse durations were prolonged or holding potentials were depolarized, the degree of the use-dependent block and Af increased. When pH was lowered to 6.5, the short pulse produced only the slow component, whereas the long pulse caused two exponentials with decreased Af and increased As. Internal application of QX-314, a permanently charged lidocaine analogue, produced a single exponential block of INa with a very slow onset rate.(ABSTRACT TRUNCATED AT 250 WORDS)
Sunami
A
A
Department of Cardiovascular Diseases, Tokyo Medical and Dental University, Japan.
Fan
Z
Z
Nitta
J
J
Hiraoka
M
M
D000818:Animals; D004206:Disopyramide / Q000494:pharmacology*; D004558:Electric Stimulation; D004594:Electrophysiology; D006168:Guinea Pigs; D006352:Heart Ventricles; D006863:Hydrogen-Ion Concentration; D008012:Lidocaine / Q000494:pharmacology*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D015222:Sodium Channels / Q000187:drug effects* / Q000502:physiology
D015222:Sodium Channels; D008012:Lidocaine; D004206:Disopyramide