Overexpression of human TRK proto-oncogene into mouse cells using an inducible vector system.
The TRK proto-oncogene encodes a tyrosine kinase receptor for an, as yet, unidentified ligand. In order to help the identification of this ligand, we have constructed an expression vector capable of overexpressing the TRK protein in an inducible fashion. We report here the characterization of the TRK proto-oncogene products obtained from this expression vector.
Mulherkar
R
R
Developmental Oncology Section, National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701.
Coulier
F
F
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015870:Gene Expression; D006801:Humans; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D008970:Molecular Weight; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D011956:Receptors, Cell Surface / Q000235:genetics*; D015183:Restriction Mapping; D014162:Transfection; D014415:Tunicamycin / Q000494:pharmacology
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011956:Receptors, Cell Surface; D014415:Tunicamycin; D008715:Methionine; D011505:Protein-Tyrosine Kinases