Expression of Epstein-Barr virus nuclear antigen 2 in insect cells from a baculovirus vector.
The open reading frame encoding the Epstein-Barr virus nuclear antigen 2 (EBNA-2) has been expressed in a recombinant baculovirus vector. The resulting product migrates with the same apparent molecular weight as EBNA-2 from latently infected or converted B cell lines. Rabbit antisera derived from the innoculation of this material immunoprecipitated EBNA-2 from cell extracts of EBV-containing cells. The high level of protein expression obtained in insect cells stands in sharp contrast to that seen in a number of mammalian cell lines using a variety of promoters including the endogenous EBNA-2 promoter, the Moloney MuLV LTR, the murine immunoglobulin heavy chain promoter, the human cytomegalovirus immediate early promoter, and the adenovirus major late promoter.
Schubach
W H
WH
Department of Medicine, State University of New York, Stony Brook 11790-8174.
Horvath
G
G
Spoth
B
B
Hearing
J C
JC
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics*; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D019309:Epstein-Barr Virus Nuclear Antigens; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000276:immunology; D015151:Immunoblotting; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007313:Insecta; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007143:Immunoglobulin Heavy Chains