[Cloning, sequencing and translation analysis of the Vaccinia virus LIVP HINDIII N genome fragment].
The sequence of vaccinia virus (VV) LIVP HindIII N DNA fragment has been determined to be 2215 b.p. Three open reading frames designated LN1, N1 and NO1 and coding polypeptides with the calculated molecular masses (32.5 kDa, 21.8 kDa and 47.8 kDa) were located. mRNAs selected by hybridization with the VV HindIII N were translated in rabbit reticulocyte lysate. Proteins of genes for host range and resistance to alpha-amanitin corresponding to 29 and 47 kDa were detected. Two forms of polypeptides of the ORF LN1 (32 and 29K), and anomalous electrophoresis mobility of the ORF LN1 are discussed.
Prikhod'ko
G G
GG
Netesova
N A
NA
Muravlev
A I
AI
Babkin
I V
IV
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics*; D011817:Rabbits; D012901:Variola virus / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis
D004279:DNA, Viral; D014764:Viral Proteins
Klonirovanie, sekvenirovanie i transliatsionnyĭ analiz HindIII-N-fragmenta genoma virusa ospovaktsiny shtamma LIVP.