title: Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase.
abstract: The nucleotide sequence of the ERG12 gene, encoding mevalonate kinase, from Saccharomyces cerevisiae is presented. The longest open reading frame may code for a protein containing 443 amino acids with a deduced relative molecular mass of 48,500. The analysis of the nucleotide sequence reveals a complete identity with the yeast gene RAR1, isolated elsewhere by complementation of a rar1 mutation involved in the stability of plasmids with weak ARS. In addition, we show that mevalonate kinase is not a rate-limiting enzyme; however its sensitivity to FFP could be a key regulatory mechanism in the sterol pathway of yeast.
authors.affiliation: Laboratoire de Biochimie et Génétique des Microorganismes, Université de Poitiers, France.
authors.forename: A
authors.initials: A
authors.lastname: Oulmouden
authors.forename: F
authors.initials: F
authors.lastname: Karst
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004875:Ergosterol / Q000096:biosynthesis; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D011106:Polyisoprenyl Phosphates / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012717:Sesquiterpenes
chemical_list: D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; C015234:geranyl pyrophosphate; C004808:farnesyl pyrophosphate; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021796:mevalonate kinase; D004875:Ergosterol