Primary cultures of guinea-pig gall-bladder epithelial cells suitable for patch-clamp studies.
Cells derived from explants of guinea-pig gall-bladder maintained in culture for up to 14 days, exhibit a network of cytoskeletal filaments that are labelled by an anti-cytokeratin antibody, but not by an anti-desmin antibody, suggesting that they are of epithelial origin. These cells proved suitable for patch-clamp studies and by using this method channels selective for K+ and Cl- were observed.
O'Brien
J A
JA
Department of Cell Biology, AFRC Institute of Animal Physiology & Genetics Research, Babraham, Cambridge.
James
P S
PS
Torre
M
M
D000818:Animals; D002478:Cells, Cultured; D018118:Chloride Channels; D004594:Electrophysiology; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005455:Fluorescent Antibody Technique; D005704:Gallbladder / Q000166:cytology / Q000502:physiology*; D006168:Guinea Pigs; D008565:Membrane Proteins / Q000502:physiology; D015221:Potassium Channels / Q000502:physiology
D018118:Chloride Channels; D008565:Membrane Proteins; D015221:Potassium Channels