The receptor kinase family: primary structure of rhodopsin kinase reveals similarities to the beta-adrenergic receptor kinase.
Light-dependent deactivation of rhodopsin as well as homologous desensitization of beta-adrenergic receptors involves receptor phosphorylation that is mediated by the highly specific protein kinases rhodopsin kinase (RK) and beta-adrenergic receptor kinase (beta ARK), respectively. We report here the cloning of a complementary DNA for RK. The deduced amino acid sequence shows a high degree of homology to beta ARK. In a phylogenetic tree constructed by comparing the catalytic domains of several protein kinases, RK and beta ARK are located on a branch close to, but separate from the cyclic nucleotide-dependent protein kinase and protein kinase C subfamilies. From the common structural features we conclude that both RK and beta ARK are members of a newly delineated gene family of guanine nucleotide-binding protein (G protein)-coupled receptor kinases that may function in diverse pathways to regulate the function of such receptors.
Lorenz
W
W
Howard Hughes Medical Institute, Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
Inglese
J
J
Palczewski
K
K
Onorato
J J
JJ
Caron
M G
MG
Lefkowitz
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D017868:Cyclic AMP-Dependent Protein Kinases; D005136:Eye Proteins; D051606:G-Protein-Coupled Receptor Kinase 1; D015870:Gene Expression; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D015183:Restriction Mapping; D012243:Rhodopsin / Q000378:metabolism; D016415:Sequence Alignment; D014162:Transfection; D051552:beta-Adrenergic Receptor Kinases
D005136:Eye Proteins; D009841:Oligonucleotides; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D012243:Rhodopsin; D011494:Protein Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D051606:G-Protein-Coupled Receptor Kinase 1; D051552:beta-Adrenergic Receptor Kinases