[Recombinant murine cell lines transformed by various vectors based on bovine papillomavirus type 1 and expressing human tissue plasminogen activator].
We have constructed a number of vectors which include transcriptional unit of human tPA cDNA and 100% BPV-1 DNA or 100% Lx DNA (mutant BPV variant with tandem duplication of LCR-E6-E7 region). Additional HSV-1 Tk-promoter was inserted in the flanks of viral DNAa in a set of constructions. A number of recombinant cell lines have been established by means of transformation using the constructed vectors. The increased focus formation activity and the improved vector properties were demonstrated for vector construction which included Lx DNA with additional Tk promoter for activation of early viral transcription. The possibilities of BPV-based vectors design are discussed.
Aleshkov
S B
SB
Ustab
M B
MB
Baev
A A
AA
D000818:Animals; D015139:Blotting, Southern; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002461:Cell Line, Transformed; D004279:DNA, Viral / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral; D005822:Genetic Vectors; D051379:Mice; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D010959:Tissue Plasminogen Activator / Q000235:genetics*
D004279:DNA, Viral; D010959:Tissue Plasminogen Activator
Rekombinantnye linii kletok myshi, transformirovannye razlichnymi vektorami na osnove bych'ego papillomavirusa tipa 1 i ékspressiruiushchie tkanevoĭ aktivator plazminogena cheloveka.