Comparison of anti-RNA properties of normal and ellipticine functionalized alpha and beta-oligonucleotides.
RNA is not cleaved as a consequence of the binding of RNase H to the duplex between RNA and a complementary alpha-oligodeoxyribonucleotide (oligo). In consequence targets have been selected which do not a priori require the action of RNase H to inhibit genetic expression. Two models have been used: The Friend Murine Leukemia Virus (F-MuLV) and the synthesis of rabbit beta globin.alpha-oligos trigger specific inhibitions in both systems. The functionalisation in 5' with the intercalating agent 9-NH2-ellipticine renders the oligos resistant to degradation and allows a direct action on cells.
Lavignon
M
M
UA 147 CNRS, U 140 INSERM, Institut G. Roussy, Villejuif, France.
Bertrand
J R
JR
Porumb
H
H
Rayner
B
B
Imbach
J L
JL
Paoletti
C
C
Malvy
C
C
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004611:Ellipticines / Q000494:pharmacology*; D005622:Friend murine leukemia virus / Q000187:drug effects / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D005914:Globins / Q000235:genetics; D007364:Intercalating Agents / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000378:metabolism / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects*; D012367:RNA, Viral / Q000187:drug effects*; D011817:Rabbits; D016914:Ribonuclease H / Q000378:metabolism*
D004611:Ellipticines; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D012367:RNA, Viral; C038373:9-aminoellipticine; D005914:Globins; D016914:Ribonuclease H