Inhibition of the rat renal Na+/H+ exchanger by beta-adrenergic antagonists.
The beta-adrenergic antagonists, alprenolol and propranolol, inhibit the Na+/H+ exchanger in rat renal brush-border membrane vesicles. Half-maximal inhibition occurs at 86 microM alprenolol and 36 microM propranolol. Similar to amiloride and Na+, propranolol protects the Na+/H+ exchanger from irreversible inhibition by the carboxyl group reagent, N,N'-dicyclohexyl-carbodiimide (DCCD). Protection is incomplete, depends on propranolol concentration, and reaches a maximum at 0.4 mM propranolol. With a comparable dose dependence, propranolol protects a 65 kDa band from labeling with [14C]DCCD. The data indicate that beta-adrenergic antagonists specifically interact with the proximal tubular Na+/H+ exchanger.
Friedrich
T
T
BASF-Ludwigshafen, F. R. G.
Burckhardt
G
G
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000526:Alprenolol / Q000494:pharmacology*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002352:Carrier Proteins / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D004024:Dicyclohexylcarbodiimide / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007672:Kidney Cortex / Q000378:metabolism*; D007700:Kinetics; D008871:Microvilli / Q000378:metabolism; D010869:Pindolol / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology*; D011485:Protein Binding; D051381:Rats; D017923:Sodium-Hydrogen Exchangers
D000319:Adrenergic beta-Antagonists; D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; D004024:Dicyclohexylcarbodiimide; D000584:Amiloride; D000526:Alprenolol; D011433:Propranolol; D010869:Pindolol