title: Activation of sympathetic tone during dipyridamole test.
abstract: Cardiac imaging with dipyridamole infusion has been proposed as an exercise-independent tool for the diagnosis of coronary artery disease. Dipyridamole acts through the accumulation of adenosine, which reduces sympathetic tone in vasomotor nuclei of the brainstem and inhibits norepinephrine release in noradrenergic neurons but also activates arterial chemoreceptors. The aim of this study was to assess whether dipyridamole administration (up to 0.84 mg/kg over 10 minutes, a dosage commonly employed for diagnostic testing) may modulate sympathetic activity either directly or indirectly through blood pressure reduction or myocardial ischemia, which may be evoked by dipyridamole infusion and represent two recognized sympathetic stimuli. Twenty patients were studied with infusion combined with two-dimensional echocardiography and 12-lead ECG monitoring. Blood pressure was recorded each minute by a cuff sphygmomanometer. In all patients, we obtained venous blood samples for epinephrine (an index of adrenomedullary catecholamine release) and norepinephrine (an index of neuronal activity) both in resting conditions and at peak dipyridamole, ie, at the first minute after termination of dipyridamole infusion in negative cases or in the presence of obvious ischemia in positive cases (ie, as soon as a regional ventricular dyssynergy or an ST segment depression greater than 0.1 mV appeared). Epinephrine and norepinephrine determinations were made by a high performance liquid chromatography (HPLC) method. After dipyridamole, there was a significant rise in norepinephrine, while epinephrine did not change significantly. Dipyridamole-induced percentage variations of norepinephrine from baseline were not significantly correlated with mean blood pressure changes (r = .1, p = ns) and were of a similar extent in patients with (n = 10) and without (n = 10) dipyridamole-induced ischemia (+68 vs +73 percent, p = ns). Dipyridamole administration provokes an activation of sympathetic tone which can be detected even in the absence of myocardial ischemia and is not related to blood pressure changes. The increased catecholamine release appears to be of neuronal rather than adrenomedullary origin.
authors.affiliation: Clinical Physiology Institute, CNR--Clinical Med I, Hypertension Unit, Pisa, Italy.
authors.forename: A R
authors.initials: AR
authors.lastname: Lucarini
authors.forename: E
authors.initials: E
authors.lastname: Picano
authors.forename: C
authors.initials: C
authors.lastname: Marini
authors.forename: S
authors.initials: S
authors.lastname: Favilla
authors.forename: A
authors.initials: A
authors.lastname: Salvetti
authors.forename: A
authors.initials: A
authors.lastname: Distante
mesh_terms: D000328:Adult; D000368:Aged; D003327:Coronary Disease / Q000097:blood / Q000175:diagnosis; D004176:Dipyridamole / Q000494:pharmacology; D004452:Echocardiography / Q000379:methods; D004562:Electrocardiography / Q000379:methods; D004837:Epinephrine / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009638:Norepinephrine / Q000097:blood; D013268:Stimulation, Chemical; D013564:Sympathetic Nervous System / Q000187:drug effects* / Q000502:physiology; D014666:Vasomotor System / Q000187:drug effects* / Q000502:physiology
chemical_list: D004176:Dipyridamole; D009638:Norepinephrine; D004837:Epinephrine