A single 3.7-kilobase messenger RNA hybridizes to immediate-early promoter enhancer of human cytomegalovirus in HL-60 and acute myeloid leukemia cells.
Expression of a mRNA cross-hybridized to human cytomegalovirus immediate-early gene promoter-enhancer was detected in the human promyelocytic leukemia cell line HL-60. The 0.6 kilobase of NruI/SacI DNA fragment of eukaryotic expression vector pCDM8 representing human cytomegalovirus immediate-early gene promoter-enhancer was used as the probe to hybridize with polyadenylated RNA by the Northern blot analysis. A 3.7-kilobase strand of polyadenylated RNA was visualized in the cytoplasmic fraction of HL-60 promyelocytes. In contrast, other human hematopoietic cell lines, hepatoma cells, and normal human fibroblasts did not show such a transcript by cross-hybridization. This transcript was called CMVE RNA. The expression of CMVE mRNA was also detectable in the fresh blast cells from patients with acute myeloid leukemia, and particularly from a patient with acute myeloid leukemia of the M3 type. Taken together, these findings suggest that the CMVE RNA-encoded gene plays an important role in the pathogenesis of acute promyelocytic leukemia.
Chang
Z F
ZF
Department of Biochemistry, Chang Gung Medical College, Taiwan, Republic of China.
Huang
D Y
DY
Lin
C J
CJ
D000208:Acute Disease; D015152:Blotting, Northern; D003587:Cytomegalovirus / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics* / Q000648:ultrastructure; D012334:RNA, Neoplasm / Q000235:genetics* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D015347:RNA Probes; D012333:RNA, Messenger; D012334:RNA, Neoplasm