Demonstration of antibodies against bovine leukemia virus (BLV) by blocking ELISA using bovine polyclonal anti-BLV immunoglobulin.
A blocking enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against bovine leukemia virus (BLV) is described. The test is based on the biotin-streptavidin system using unlabelled polyclonal bovine IgG against BLV as catching antibody and biotinylated bovine anti-BLV IgG as detecting antibody. The sensitivity was found to be 50-100 times higher than the agar gel immunodiffusion test, with a specificity of practically 100%. The blocking ELISA proved to be suitable for detection of antibodies against BLV in serum and milk. In 34 paired milk/serum samples, the average ratio of BLV antibody titres was 1:26. So far, more than 700,000 sera have been screened by blocking ELISA for BLV antibodies in the course of the Danish surveillance programme for BLV infection.
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State Veterinary Institute for Virus Research, Kalvehave, Denmark.
Hoff-Jørgensen
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D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D001667:Binding, Competitive; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000276:immunology*; D007938:Leukemia / Q000276:immunology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008892:Milk / Q000276:immunology; D011237:Predictive Value of Tests; D012044:Regression Analysis
D000914:Antibodies, Viral; D007074:Immunoglobulin G