title: Nuclear protein kinases in rat liver: evidence for increased histone H1 phosphorylating activity during liver regeneration.
abstract: Comparison of protein kinase activity in normal and regenerating rat liver nuclei indicates that exogenous histone H1 is hyperphosphorylated in 22-h regenerating nuclei. The protein kinase involved is not sensitive to protein kinase A inhibitor, is inhibited by staurosporine and by an anti-PKC polyclonal antibody, utilizes only ATP, and also phosphorylates the C-terminal fragment of histone H1. These data suggest that protein kinase C is responsible for the observed effects, in agreement with the presence of this enzyme in normal and regenerating nuclei demonstrated by immunoblotting.
authors.affiliation: Istituto di Anatomia Umana Normale, Università di Bologna Ferrara, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Martelli
authors.forename: C
authors.initials: C
authors.lastname: Carini
authors.forename: S
authors.initials: S
authors.lastname: Marmiroli
authors.forename: M
authors.initials: M
authors.lastname: Mazzoni
authors.forename: P J
authors.initials: PJ
authors.lastname: Barker
authors.forename: R S
authors.initials: RS
authors.lastname: Gilmour
authors.forename: S
authors.initials: S
authors.lastname: Capitani
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D006657:Histones / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D019311:Staurosporine; D013379:Substrate Specificity; D013997:Time Factors
chemical_list: D000470:Alkaloids; D006657:Histones; D009687:Nuclear Proteins; D010750:Phosphoproteins; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D011493:Protein Kinase C; D010749:Phosphoprotein Phosphatases; D019311:Staurosporine