title: [Phosphorylation of myocardial sarcolemma proteins during induction of the phosphatidylinositol cycle in isolated perfused rabbit heart].
abstract: Phosphorylation of cardiac sarcolemma proteins under stimulation of M-receptors by agonist carbacholine used to stimulate phosphatidylinositide cycle, was investigated in the isolated, rabbit heart perfused with 32Pi. Carbacholine (10(-7) stimulates the polyphosphoinositide metabolism which is expressed in the activated incorporation of 32P from [gamma-32P]ATP in polyphosphoinositide as well as in the increased content of the labelled inositol trisphosphate released through phosphatidylinositol-4,5-bisphosphate break-down by phospholipase C. The diacylglycerol produced simultaneously with inositol triphosphate as a second messenger activates the protein kinase C. This was confirmed by considerable activation of phosphorylation sarcolemma proteins-substrates of protein kinase C, with Mr 94, 87, 78, 51 and 46 kDa.
authors.forename: M D
authors.initials: MD
authors.lastname: Kurskiĭ
authors.forename: V I
authors.initials: VI
authors.lastname: Kocherga
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Puzyrev
authors.forename: N V
authors.initials: NV
authors.lastname: Nesterenko
authors.forename: L K
authors.initials: LK
authors.lastname: Kurchenko
authors.forename: V A
authors.initials: VA
authors.lastname: Afanas'ev
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004075:Diglycerides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D011817:Rabbits; D012508:Sarcolemma / Q000201:enzymology / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D010716:Phosphatidylinositols; D000255:Adenosine Triphosphate; D002217:Carbachol; D011493:Protein Kinase C
vernacular_title: Fosforilirovanie belkov sarkolemmy miokarda pri induktsii fosfatidilinozitidnogo tsikla v izolirovannom perfuziruemom serdtse krolika.