[Evidence for Campbell's mechanism for fine excision of Tn9 type transposons in Escherichia coli K12].
The plasmid-transposon Tn9-322 was constructed by inverted transposition from the pBR322::Tn9 plasmid. The precise excision of the Tn9-322 transposon from the proB gene site can proceed by the Campbell's model. This fact was demonstrated by appearance of the plasmid-transposons after their precise excision. They contain two IS1 elements flanking a short direct repeat of the target DNA. The recombinational mechanism of precise excision of Tn9 type transposons seems not to be alternative but looks as an additional one to a well-known slippage mechanism proved for Tn5 and Tn10.
Goryshin
I Iu
IIu
Tamm
S E
SE
Lantsov
V A
VA
D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids
D004251:DNA Transposable Elements; D004269:DNA, Bacterial
Dokazatel'stvo kempbellovskogo mekhanizma tochnoĭ ékstsizii sostavnogo transpozona tipa Tn9 Escherichia coli K12.