title: The contribution of arachidonic acid to the aetiology and pathophysiology of focal brain oedema; studies using an infusion oedema model.
abstract: Arachidonic acid solution (2 to 15 mg/ml) was infused into the right forebrain white matter of anaesthetised cats over three hours to evaluate its contribution to the genesis and pathophysiology of vasogenic brain oedema. The 0.6 ml infusion increased local white matter water content by a mean of 11.3 ml/100 g tissue but did not increase cortical water content. Histological studies revealed local expansion and trabeculation of the white matter with aggregations of granulocytic neutrophils in the venules and perivenular brain. The adjacent cortical cytoarchitecture was normal. The white matter around the infusion site was stained lightly and over a variable area (15-20 mm2) by intravenously administered Evans Blue dye 2%. Regional cerebral blood flow (rCBF) adjacent to the frontal infusion did not change significantly during the period of infusion and remained similar to rCBF in the contralateral hemisphere. Following the arachidonic acid infusion regional CBF CO2 reactivity was normal and three was no asymmetry of either cortical somatosensory evoked potential (SEP) or motor evoked potential (MEP) waveforms. The increase in brain water content and changes in the ICP and ICP related biodynamics (pressure-volume index, lumped craniospinal compliance and CSF outflow resistance) were similar to those seen following infusion of 0.6 ml saline. These studies suggest that free intraparenchymal arachidonic acid, at concentrations exceeding those occurring in most neuropathological conditions, can increase the normal brain parenchymal capillary permeability but does not disrupt focal cerebrovascular and electrophysiological function. The clinical implications of these findings are discussed.
authors.affiliation: Department of Clinical Neuroscience (Surgical Neurology), Western General Hospital, Edinburgh, Scotland.
authors.forename: I R
authors.initials: IR
authors.lastname: Whittle
authors.forename: I R
authors.initials: IR
authors.lastname: Piper
authors.forename: J D
authors.initials: JD
authors.lastname: Miller
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000502:physiology*; D001812:Blood-Brain Barrier / Q000502:physiology*; D001929:Brain Edema / Q000473:pathology / Q000503:physiopathology*; D002415:Cats; D002540:Cerebral Cortex / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology; D015170:Cerebrospinal Fluid Pressure / Q000502:physiology; D004195:Disease Models, Animal; D005073:Evoked Potentials, Somatosensory / Q000502:physiology; D007427:Intracranial Pressure / Q000502:physiology; D007975:Leukotriene B4 / Q000502:physiology; D009132:Muscles / Q000294:innervation; D011930:Reaction Time / Q000502:physiology; D012039:Regional Blood Flow / Q000502:physiology; D013189:SRS-A / Q000502:physiology; D013046:Specific Gravity; D009435:Synaptic Transmission / Q000502:physiology; D014655:Vascular Resistance / Q000502:physiology
chemical_list: D013189:SRS-A; D007975:Leukotriene B4; D016718:Arachidonic Acid