title: Amino acid sequences of Euglena viridis ferredoxin and cytochromes c.
abstract: The Order Euglenida comprises many species and perhaps 40 genera, but almost all biochemical and genetic studies have been limited to a single species. Euglena gracilis, because of its ease of growth in the laboratory. Sequence studies of chloroplast and mitochondrial proteins from E. gracilis show that they have diverged widely from other eukaryotic lines. In the present paper we report the sequences of three proteins from another euglenoid, Euglena viridis, using material isolated from a natural bloom. The mitochondrial cytochrome c shows more than 90% sequence identity with that from E. gracilis, and contains the same characteristic features. The chloroplast cytochrome c6 has diverged to a greater extent and shows only 77% identity. The chloroplast ferredoxin from E. viridis is similar in sequence to those of cyanobacteria and algal chloroplasts, with sequence identities of up to 75%. Details of the purification, analysis and sequence determination experiments on the peptides have been deposited as Supplementary Publication SUP 50163 (32 pages) at the British Library Document Supply Centre, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1991) 273, 5.
authors.affiliation: Department of Molecular Biology, University of Edinburgh, Scotland, U.K.
authors.forename: R P
authors.initials: RP
authors.lastname: Ambler
authors.forename: M D
authors.initials: MD
authors.lastname: Kamen
authors.forename: R G
authors.initials: RG
authors.lastname: Bartsch
authors.forename: T E
authors.initials: TE
authors.lastname: Meyer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002736:Chloroplasts / Q000378:metabolism; D003574:Cytochrome c Group / Q000235:genetics* / Q000302:isolation & purification; D010450:Endopeptidases; D005055:Euglena / Q000235:genetics* / Q000378:metabolism; D005288:Ferredoxins / Q000235:genetics* / Q000302:isolation & purification; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003574:Cytochrome c Group; D005288:Ferredoxins; D010446:Peptide Fragments; D010450:Endopeptidases