Receptor-mediated changes in intracellular [Ca2+] in cultured rat brain capillary endothelial cells.
Agents which are known to alter blood-brain barrier permeability have been tested for their ability to raise [Ca2+]i in rat brain capillary endothelial cells loaded with the calcium indicator Fura-2, and subjected to dual wavelength excitation. The cells show increases in [Ca2+]i in response to bradykinin, histamine and ATP. Possible sources of this calcium are discussed.
Revest
P A
PA
Biomedical Sciences Division, King's College London, U.K.
Abbott
N J
NJ
Gillespie
J I
JI
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D001812:Blood-Brain Barrier; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002196:Capillaries; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology*; D007700:Kinetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology*; D011983:Receptors, Purinergic / Q000187:drug effects / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology
D000227:Adenine Nucleotides; D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; D012701:Serotonin; D006632:Histamine; D001920:Bradykinin; D002118:Calcium