title: A cell culture model of the blood-brain barrier.
abstract: Endothelial cells that make up brain capillaries and constitute the blood-brain barrier become different from peripheral endothelial cells in response to inductive factors found in the nervous system. We have established a cell culture model of the blood-brain barrier by treating brain endothelial cells with a combination of astrocyte-conditioned medium and agents that elevate intracellular cAMP. These cells form high resistance tight junctions and exhibit low rates of paracellular leakage and fluid-phase endocytosis. They also undergo a dramatic structural reorganization as they form tight junctions. Results from these studies suggest modes of manipulating the permeability of the blood-brain barrier, potentially providing the basis for increasing the penetration of drugs into the central nervous system.
authors.affiliation: Athena Neurosciences, Inc., South San Francisco, California 94080.
authors.forename: L L
authors.initials: LL
authors.lastname: Rubin
authors.forename: D E
authors.initials: DE
authors.lastname: Hall
authors.forename: S
authors.initials: S
authors.lastname: Porter
authors.forename: K
authors.initials: K
authors.lastname: Barbu
authors.forename: C
authors.initials: C
authors.lastname: Cannon
authors.forename: H C
authors.initials: HC
authors.lastname: Horner
authors.forename: M
authors.initials: M
authors.lastname: Janatpour
authors.forename: C W
authors.initials: CW
authors.lastname: Liaw
authors.forename: K
authors.initials: K
authors.lastname: Manning
authors.forename: J
authors.initials: J
authors.lastname: Morales
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D001692:Biological Transport; D001812:Blood-Brain Barrier; D002417:Cattle; D002478:Cells, Cultured; D002999:Clone Cells; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004705:Endocytosis; D004727:Endothelium / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D051381:Rats
chemical_list: D003470:Culture Media; D000242:Cyclic AMP; D006152:Cyclic GMP