Detection of APC region-specific signals by nonisotopic chromosomal in situ suppression (CISS)-hybridization using a microdissection library as a probe.
The chromosome region 5q22 harbouring the putative gene associated with adenomatous polyposis coli (APC) was microdissected and microcloned from GTG-banded human metaphase chromosomes. In order to determine the precise regional localization of the microdissected material, we used polymerase chain reaction amplified microclones as a bulk-probe in nonradioactive chromosomal in situ suppression hybridization of human metaphase spreads. Specific in situ hybridization signals were obtained on the long arm of chromosome 5 in accordance with the chromosomal region excised for the cloning procedure. The application of this detection system should provide a rapid and powerful tool for analyzing patients with translocations or microdeletions of a given chromosome region.
Trautmann
U
U
Institut für Humangenetik der Universität, Erlangen, Federal Republic of Germany.
Leuteritz
G
G
Senger
G
G
Claussen
U
U
Ballhausen
W G
WG
D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002895:Chromosomes, Human, Pair 5; D015342:DNA Probes; D015698:Genomic Library; D006801:Humans; D007621:Karyotyping; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic
D015342:DNA Probes