Reduction of cytochrome C by ascorbic free radical.
The rate of "in vivo" reduction of cytochrome c by ascorbic acid (AA) increases from 69 nmoles of cytochrome c for minute, to 202 nanomoles when ascorbate oxidase is added. Since the AA oxidation by AA oxidase is a system to generate ascorbic free radical (AFR), data suggest that AFR is a better reducing compound than ascorbate in cytochrome c reduction. Since the addition of oxidized glutathione and human immunoglobulins (-S-S- bridge containing compounds) in the medium produces a remarkable decrease in cytochrome c reduction, it is suggested that AFR could also reduce -S-S- groups.
Paciolla
C
C
Istituto di Botanica dell'Università di Bari.
De Gara
L
L
D001204:Ascorbate Oxidase / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D005609:Free Radicals; D005978:Glutathione / Q000494:pharmacology; D006801:Humans; D007136:Immunoglobulins / Q000494:pharmacology; D007700:Kinetics; D010084:Oxidation-Reduction
D003574:Cytochrome c Group; D005609:Free Radicals; D007136:Immunoglobulins; D001204:Ascorbate Oxidase; D005978:Glutathione; D001205:Ascorbic Acid