Non-viral heterogeneous sequences at the 5' ends of tomato spotted wilt virus mRNAs.
Subgenomic messenger RNAs transcribed from the tomato spotted wilt virus (TSWV) S RNA segment were partially purified from total RNA extracts of TSWV-infected Nicotiana rustica and analysed by primer extension analysis. The data obtained show the presence of non-viral sequences, 12 to 20 nucleotides in length, at the 5' ends of the N and NSs mRNAs, indicating a cap-snatching mechanism for the initiation of transcription. This is the first report of a plant virus using such a mechanism for transcription of the viral genome.
Kormelink
R
R
Department of Virology, Wageningen, The Netherlands.
van Poelwijk
F
F
Peters
D
D
Goldbach
R
R
D010942:Plant Viruses / Q000235:genetics*; D010947:Plants, Toxic; D012315:RNA Caps / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014026:Nicotiana / Q000382:microbiology; D014158:Transcription, Genetic / Q000235:genetics*
D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral