title: Parental-specific methylation of an imprinted transgene is established during gametogenesis and progressively changes during embryogenesis.
abstract: Genomic imprinting is a regulatory process that requires a cell to recognize the parental origin of alleles. To understand how these alleles are distinguished, we have assessed changes in the DNA methylation of an imprinted transgene as it switches from one inheritance pattern to another while moving through gametogenesis and embryogenesis. We find that both maternally and paternally inherited methylation patterns are erased in primordial germ cells and that distinctive patterns emerge during germ cell maturation. In the case of the maternal allele, the methylation pattern is fully acquired during oogenesis. In the case of the paternal allele, the methylation pattern found in sperm undergoes further modification during embryogenesis. Thus, the distinction between "erased" maternal and paternal alleles is first established during their residence in different germ cells and then may be maintained by the recognition of the distinctive patterns that each allele displays in the zygote.
authors.affiliation: Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors.forename: J R
authors.initials: JR
authors.lastname: Chaillet
authors.forename: T F
authors.initials: TF
authors.lastname: Vogt
authors.forename: D R
authors.initials: DR
authors.lastname: Beier
authors.forename: P
authors.initials: P
authors.lastname: Leder
mesh_terms: D000483:Alleles; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D005718:Gametogenesis / Q000235:genetics*; D016259:Genes, myc; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008297:Male; D008745:Methylation; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009866:Oogenesis; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D007143:Immunoglobulin Heavy Chains; D004247:DNA