An additional homolog of the fission yeast cdc25+ gene occurs in humans and is highly expressed in some cancer cells.
The gene cdc25+ is a mitotic inducer controlling transition from the G2 to the M phase of the cell cycle in the fission yeast, Schizosaccharomyces pombe. Using phenotypic complementation of a mutant of S. pombe, we have cloned a human homolog (CDC25Hu2) of the cdc25+ gene that differs markedly in structure from CDC25 (referred to here as CDC25Hu1), the first such homolog to be isolated. The carboxyl-terminal region of p63CDC25Hu2 shares significant sequence similarity with cdc25 protein homologs from other eukaryotes and possesses full complementation activity. CDC25Hu2 is expressed in human cell lines 10 to 100 times more than CDC25Hu1, and its expression is particularly high in some cancers, including SV40-transformed fibroblasts. Whereas CDC25Hu1 is predominantly expressed in G2, CDC25Hu2 is expressed throughout the cell cycle with a moderate increase in G2. Thus, at least two homologs of the cdc25 gene exist and are both expressed in human cells. The implications of CDC25Hu2 overexpression in some cancer cells are discussed.
Nagata
A
A
Department of Molecular Genetics, Osaka University, Japan.
Igarashi
M
M
Jinno
S
S
Suto
K
K
Okayama
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000502:physiology; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005796:Genes; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000502:physiology; D011506:Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013539:Simian virus 40 / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000378:metabolism; D020687:cdc25 Phosphatases; D020666:ras-GRF1
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D009363:Neoplasm Proteins; D011506:Proteins; D020666:ras-GRF1; D004247:DNA; D010749:Phosphoprotein Phosphatases; C517008:CDC25B protein, human; D020687:cdc25 Phosphatases