Absorption, distribution and excretion of the colour fraction of Caramel Colour IV in the rat.
Caramel Colour IV prepared from [U-14C]glucose was ultrafiltered in order to isolate the high molecular weight colour fraction (HMCF). The colour fraction that was non-permeable to a 10,000-Da porosity membrane, contained 84% of the colour, 22% of the solids and 24% of the radioactivity of the [14C]Caramel Colour IV. The absorption, distribution and excretion of [14C]HMCF were evaluated in male rats after administration of single or multiple oral doses of the material at a dosage level of 2.5 g/kg body weight. Rats on the multiple oral dosage regimen were given unlabelled HMCF in their drinking water for 13 days before the administration of a bolus dose of [14C]HMCF on day 14. On both dosage regimens, the predominant route of excretion was by way of the faeces. Less than 3% of the administered radioactivity was excreted in the urine and only a negligible amount was found in the expired air. More than 99% of the administered radioactivity was excreted within 96 hr. The principal tissues in which radioactivity was found were the mesenteric lymph nodes, liver, kidney and tissues of the gastro-intestinal tract. No major differences were observed in the absorption, distribution or excretion patterns between the single and multiple oral dose regimens.
Selim
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Biological Test Center, American McGaw, Irvine, CA 92714.
Chappel
C I
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Schoenig
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D000042:Absorption; D000284:Administration, Oral; D000818:Animals; D002182:Candy; D002241:Carbohydrates; D004326:Drinking; D005243:Feces / Q000737:chemistry; D005505:Food Coloring Agents / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D007420:Intestine, Large / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D008297:Male; D008643:Mesentery; D009930:Organic Chemicals; D051381:Rats; D011916:Rats, Inbred F344; D014018:Tissue Distribution
D002241:Carbohydrates; D005505:Food Coloring Agents; D009930:Organic Chemicals; C569050:caramel coloring