Cell-cycle dependent mutation of human lymphoblasts: bromodeoxyuridine and butyl methanesulfonate.
Cells of the human lymphoblast line WI-L2 and its derivative TK-6 were synchronized by centrifugal elutriation and cell-cycle dependent mutation to 6TGR (HPRT) and OUAR (Na+, K+ ATPase) measured. Bromodeoxyuridine induced 6TGR and OUAR mutations within S phase while butylmethyl-sulfonate induced mutation displayed no cell-cycle dependence. The data indicate that centrifugal elutriation is a facile means to obtain a useful degree of synchrony for these cell lines.
Hoppe
H
H
Massachusetts Institute of Technology, Center for Environmental Health Sciences, Cambridge 02139.
Call
K M
KM
Leong
P M
PM
Thilly
W G
WG
D001973:Bromodeoxyuridine / Q000633:toxicity*; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D002498:Centrifugation; D004247:DNA / Q000096:biosynthesis; D008214:Lymphocytes / Q000166:cytology; D008698:Mesylates / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation
D008698:Mesylates; D009153:Mutagens; C018011:butyl methanesulfonate; D004247:DNA; D001973:Bromodeoxyuridine