Precise excision of Tn10 in Salmonella typhimurium: effects of mutations in the polA, dam, mutH and mutB genes and of methionine or ethionine in the plating medium.
Precise excision of Tn10 occurs at significantly elevated frequencies in cultures of the polA7 mutant strain of Salmonella typhimurium, is further increased in polA7 dam-1 and polA7 mutB strains and decreased in a polA7 mutH background. The numbers of precise excision events occurring in polA7 strains are also significantly increased when methionine (20 micrograms/ml or less) is present in the medium but decreased when ethionine (again, 20 micrograms/ml or less) is present. When both amino present, the outcome is about a 2-fold increase in precise excision events. The involvement of mismatch repair and methylation patterns in precise excision events is discussed.
Hafner
L M
LM
Department of Microbiology, La Trobe University, Bundoora, Vic., Australia.
MacPhee
D G
DG
D004260:DNA Repair; D004251:DNA Transposable Elements / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D005001:Ethionine / Q000494:pharmacology*; D005798:Genes, Bacterial; D008715:Methionine / Q000494:pharmacology*; D008745:Methylation; D009154:Mutation; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D008715:Methionine; D005001:Ethionine