Properties of human parainfluenza virus type 3 RNA polymerase/replicase activity in vitro: consensus with other negative-stranded RNA viruses.
A cell-free system supporting transcription, replication, and nucleocapsid assembly of the genome RNA of human parainfluenza virus type 3 (HPF3) is described. Cytoplasmic extracts from infected CV-1 or BHK cells catalyzed the transcription of the entire HPF3 genome, the replication of genome RNA, and the assembly of this RNA into nucleocapsidlike structures. Newly replicated RNA was resistant to micrococcal nuclease digestion and was stable in CsCl gradients, exhibiting the density of authentic HPF3 nucleocapsids. After fractionation of the extracts, the nucleocapsid-containing pellet fraction synthesized viral mRNAs. Reconstitution with the soluble protein fraction was necessary for genome RNA replication and nucleocapsid assembly.
Moscona
A
A
Department of Pediatrics, Mount Sinai School of Medicine, New York, New York 10029-6574.
Peluso
R W
RW
D000818:Animals; D002213:Capsid / Q000096:biosynthesis; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D005591:Chemical Fractionation; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008836:Micrococcal Nuclease / Q000378:metabolism; D010224:Parainfluenza Virus 3, Human / Q000201:enzymology* / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000096:biosynthesis
D012367:RNA, Viral; D014758:Viral Core Proteins; D012324:RNA-Dependent RNA Polymerase; D012321:DNA-Directed RNA Polymerases; D008836:Micrococcal Nuclease