The effects of radiation and alkylating agents on chromatin degradation in normal and tumour lymphoid cells.
The dynamic of chromatin degradation was studied in thymocytes and LS/BL tumour cells. In permeabilised LS/BL cells, the rate of DNA degradation induced by endogenous calcium and magnesium-dependent endonuclease was approx. 25 times slower than in thymocytes. In LS/BL cells irradiation does not induce chromatin degradation. The alkylating agent TS 160 induced chromatin degradation in both LS/BL lymphosarcoma cells and thymocytes.
Ryabchenko
N I
NI
Research Institute of Medical Radiology, Academy of Medical Sciences of the USSR, Obninsk.
Jurásková
V
V
Ivannik
B P
BP
Konov
A V
AV
Drásil
V
V
D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004720:Endonucleases / Q000378:metabolism; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology*; D051381:Rats; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D014407:Tumor Cells, Cultured
D000477:Alkylating Agents; D002843:Chromatin; D009588:Nitrogen Mustard Compounds; C009802:2,2',2''-trichlorotriethylamine; D004720:Endonucleases; D008274:Magnesium; D002118:Calcium