Difficulties encountered in the detection of nitric oxide (NO) by spin trapping techniques. A cautionary note.
The spin trapping technique was used in an attempt to detect the free radical nitric oxide (NO) in solution. Five different spin traps were examined, alpha-phenyl-N-tert butyl nitrone (PBN), alpha-(4-pyridyl-N-oxide) N-tert-butylnitrone (POBN), 5,5-dimethyl-pyrroline-N-oxide (DMPO), 2-methyl-2-nitrosopropane (MNP) and 3,5-dibromo-4-nitrosobenzene sulfonate (DBNBS). Our results suggest that the nitroso spin traps (MNP, DBNBS) are better suited for the identification of NO-related signals, than the nitrones, DMPO, PBN and POBN. In addition, it is shown that spin trapping of NO-related signals with nitroso and nitrone spin traps is subject to many artifacts.
Arroyo
C M
CM
Veterans Administration Medical Center, Baltimore, Maryland 21218.
Kohno
M
M
D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals; D009569:Nitric Oxide / Q000032:analysis*; D009587:Nitrogen Isotopes; D009603:Nitroso Compounds; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005609:Free Radicals; D009587:Nitrogen Isotopes; D009603:Nitroso Compounds; D013113:Spin Labels; D009569:Nitric Oxide; C017245:5,5-dimethyl-1-pyrroline-1-oxide; C034526:tert-nitrosobutane