title: Crystallization and preliminary X-ray diffraction study of cytochrome c552 from Hydrogenobacter thermophilus.
abstract: Cytochrome c552 from a thermophilic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus, exhibits remarkable thermostability. The oxidized cytochrome c552 has been crystallized in an ethanol/water mixture by means of the vapor diffusion method. The crystals belong to the orthorhombic system, space group P2(1)2(1)2, with unit cell dimensions of a = 93.4 A, b = 52.9 A, and c = 32.4 A. Most probably the asymmetric unit contains two molecules of cytochrome c552. The crystals diffract X-rays to better than 2.5 A resolution and are stable to X-ray irradiation.
authors.affiliation: Department of Biology, Faculty of Science, Osaka University.
authors.forename: K
authors.initials: K
authors.lastname: Fukuyama
authors.forename: H
authors.initials: H
authors.lastname: Matsubara
authors.forename: Y
authors.initials: Y
authors.lastname: Sanbongi
authors.forename: T
authors.initials: T
authors.lastname: Kodama
mesh_terms: D001420:Bacteria, Aerobic / Q000737:chemistry*; D003460:Crystallization; D003574:Cytochrome c Group / Q000737:chemistry*; D006358:Hot Temperature; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: D003574:Cytochrome c Group; C083951:cytochrome c553; C022302:cytochrome C-552