[Membranotropic effect of phosphocreatine and its structural analogs].
The effects of phosphocreatine (PCr) and its analogues (creatine, phosphocreatinine, phosphoarginine and inorganic phosphate) on liposomal and erythrocyte membranes and on the sarcolemmal membrane of cardiomyocytes were studied. The ESR spectrum of the spin-labeled probe, 5-doxyl-stearate, incorporated into the membrane were recorded for analysis of the structural order of the phospholipid bilayer of these membranes. PCr and its analogues had no effect on the structure of the phospholipid bilayer in liposomes; this effect was temperature-independent. However, in erythrocyte and sarcolemmal membranes the rigidity of the membranes was increased by these compounds (except for creatine) at temperatures above 38-40 degrees C. Analysis of these and literary data revealed that cardiac cell membranes may be the site of protective action of PCr on the ischemic myocardium. The lack of effect on liposomes may suggest that the membrane-stabilizing effect of PCr depends on the presence of membrane proteins. The compounds under study may influence the lipid-protein interactions by increasing the rigidity of membrane phospholipids. These membranotropic effects may be due to the interaction of charged molecules of the compounds with polar heads of phospholipids and/or polar groups of proteins in the membrane interphase which, in turn, may influence the packing of hydrophobic fatty acid chains.
Konorev
E A
EA
Medvedeva
N V
NV
Dzhaliashvili
I V
IV
Stepanov
V A
VA
Saks
V A
VA
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D004285:Dogs; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010725:Phosphocreatine / Q000031:analogs & derivatives / Q000494:pharmacology*; D012508:Sarcolemma / Q000187:drug effects / Q000378:metabolism; D013696:Temperature
D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids; D010725:Phosphocreatine
Membranotropnoe deĭstvie fosfokreatina i ego strukturnykh analogov.