Construction of cloning vectors for Bacillus thuringiensis.
The replication region of the Bacillus thuringiensis plasmid, pHT1030, was treated with hydroxylamine. Various copy-number mutants were selected and subsequently used to construct shuttle vectors with multiple cloning sites. These recombinant plasmids are very stable and allowed the cloning of a delta-endotoxin-encoding gene in B. thuringiensis. Comparison between gene expression level and vector copy-number indicated that a plateau in delta-endotoxin production is reached with a copy-number of about fifteen per equivalent chromosome.
Arantes
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Unité de Biochimie Microbienne, URA 1300, Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.
Lereclus
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D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003001:Cloning, Molecular; D004261:DNA Replication / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006460:Hemolysin Proteins; D019811:Hydroxylamine; D006898:Hydroxylamines; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D006898:Hydroxylamines; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D019811:Hydroxylamine