title: NMR study of seed hydration with deuterated water: dependence of proton signals on hydration level.
abstract: Proton NMR signals in seeds are shown to depend on hydration level. In fact at low water amount, as it occurs in many native seeds, protons can have a restricted mobility and are not detectable. A NMR method for measuring the dependence of proton signals on hydration is reported. The method also allows the separation of the contributions of water and non-water protons in a low-resolution NMR experiment. It is based on successive hydrations (with deuterated water) - desiccation steps and on the analysis of the transverse magnetization decay curves.
authors.affiliation: Department of Chemistry, University of Rome, La Sapienza, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Di Nola
authors.forename: A
authors.initials: A
authors.lastname: D'Ubaldo
authors.forename: M
authors.initials: M
authors.lastname: Fracassi
authors.forename: E
authors.initials: E
authors.lastname: Brosio
mesh_terms: D002451:Cell Compartmentation; D003903:Deuterium; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons; D012639:Seeds; D014867:Water / Q000032:analysis*
chemical_list: D011522:Protons; D014867:Water; D003903:Deuterium