title: Degradation of mitochondrial phospholipids during experimental cerebral ischemia in rats.
abstract: Changes in content of brain mitochondrial phospholipids were examined in rats after 30 and 60 min of decapitation ischemia compared with controls, to explore the degradation of the mitochondrial membrane and its relation to dysfunction of mitochondria. Activities of respiratory functions and respiratory enzymes (cytochrome c oxidase; F0F1-ATPase) decreased significantly during ischemia. Considerable decreases in cardiolipin and phosphatidylinositol content were observed after 60 min, and other phospholipids showed similar but nonsignificant decreases in content. The amount of polyunsaturated fatty acids chains, such as arachidonic and docosahexaenoic acids, was reduced in each phospholipid, in some cases significantly, after 30 and 60 min of ischemia in time-dependent manners. Degradation of mitochondrial phospholipids during ischemia associated with the deterioration of mitochondrial respiratory functions suggested the significance of such changes in phospholipid content in disintegration of cellular energy metabolism during cerebral ischemia.
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, Kyoto University, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Nakahara
authors.forename: H
authors.initials: H
authors.lastname: Kikuchi
authors.forename: W
authors.initials: W
authors.lastname: Taki
authors.forename: S
authors.initials: S
authors.lastname: Nishi
authors.forename: M
authors.initials: M
authors.lastname: Kito
authors.forename: Y
authors.initials: Y
authors.lastname: Yonekawa
authors.forename: Y
authors.initials: Y
authors.lastname: Goto
authors.forename: N
authors.initials: N
authors.lastname: Ogata
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D003576:Electron Transport Complex IV / Q000378:metabolism / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000378:metabolism / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D010743:Phospholipids; D003576:Electron Transport Complex IV; D006180:Proton-Translocating ATPases