title: Bca77I: a new type-II restriction endonuclease from Bacillus caldolyticus cutting at the (A/T) increases CCGG(A/T) site.
abstract: We describe a new restriction enzyme recognizing a degenerate hexanucleotide sequence and cleaving the 5'-W increases CCGGW site (W = A or T). This enzyme cuts with high efficiency all four permutations of this sequence; ACCGGA (TCCGGT on the opposite strand), ACCGGT and TCCGGA. Methylation of the external cytosine completely blocks restriction while methylation of the internal cytosine only decreases the rate of restriction activity.
authors.affiliation: Promega Corporation, Madison, WI 53711.
authors.forename: T
authors.initials: T
authors.lastname: Schoenfeld
authors.forename: K B
authors.initials: KB
authors.lastname: King
authors.forename: M
authors.initials: M
authors.lastname: Schink
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D008745:Methylation; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C075961:WCCGGW-specific type II deoxyribonucleases