Characterization of MR (P) strains of Drosophila melanogaster: the number of intact P elements and their genetic effect.
To study the effect of mutagenic/carcinogenic agents on P-element transposition, the P strains used should be defined, especially with respect to the number of intact and functional P elements present. In this investigation, the relation between the number of complete P elements present in dysgenic males and P-insertion mutagenesis was studied in several MR (P) strains. The main conclusions from this investigation are: (1) Complete P elements can be present in the genome without genetic activity (even in a 'dysgenic' cross). As a consequence, the number of complete P elements present in particular dysgenic flies, is not necessarily an indication of their dysgenic genetic activity. (2) The MR-h12/Cy strain carries two complete P elements, one on the X chromosome without and one on the MR chromosome with genetic activity (making this strain most suitable for studies on P-transposition mechanisms).
Eeken
J C
JC
Department of Radiation Genetics, Sylvius Laboratories, State University of Leiden, The Netherlands.
Romeyn
R J
RJ
de Jong
A W
AW
Yannopoulos
G
G
Pastink
A
A
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics*; D015183:Restriction Mapping; D014960:X Chromosome
D004251:DNA Transposable Elements