Double-antibody sandwich ELISA for detection of infectious bursal disease virus.
A double-antibody sandwich ELISA was employed for detection of IBD virus in bursal suspension. Fifty IBD-free white leghorn chickens aged 5 weeks were experimentally infected with IBD virus. Bursae were collected 4, 8, 12, 24, 36 and 48 hours and 3, 4 and 5 days post-infection. An equal number of chickens acted as appropriate controls. The colour difference between a positive and a negative reaction was clearly distinguished with the naked eye. The cut-off level between ELISA negative and ELISA positive absorbance values was estimated at mean absorbance of negative controls plus three times the standard deviation.
Kumar
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Department of Pathology, Orissa Veterinary College, Bhubaneswar, India.
Rao
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AT
D000818:Animals; D002060:Bursa of Fabricius / Q000382:microbiology*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D007243:Infectious bursal disease virus / Q000302:isolation & purification*; D011201:Poultry Diseases / Q000382:microbiology*; D011237:Predictive Value of Tests; D012088:Reoviridae Infections / Q000382:microbiology / Q000662:veterinary*