Mouse hepatitis virus (MHV-4, JHM) blocks gamma-interferon-induced major histocompatibility complex class II antigen expression on murine cerebral endothelial cells.
The regulation of gamma-interferon-induced major histocompatibility complex (MHC) class II antigen expression on mouse cerebral endothelial cells by the neurotropic mouse hepatitis virus (MHV-4, JHM) was studied in vitro. The results presented demonstrate that MHV-4 can selectively block gamma-interferon-induced class II antigen expression on cerebral endothelial cells. The blocking effect of class II expression occurs in a strain-dependent manner, and is limited to virus-susceptible mouse strains. Virus replication is not required to obtain the blocking effect since UV-inactivated MHV-4 produces the same result. MHV-4 blocking of gamma-interferon-induced class II antigen expression is observed at both the cell surface (flow cytometry) and transcriptional level (Northern analysis).
Joseph
J
J
Department of Neurology, Jefferson Medical College, Philadelphia, PA 19107.
Knobler
R L
RL
Lublin
F D
FD
Hart
M N
MN
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001921:Brain / Q000098:blood supply* / Q000166:cytology / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology*; D015870:Gene Expression; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000276:immunology; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology*; D006517:Murine hepatitis virus / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors; D014158:Transcription, Genetic; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000528:radiation effects
D000906:Antibodies; D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D007371:Interferon-gamma