[Use of transposons for studying the genetic organization of Vibrio cholerae non 01].
The nonagglutinating vibrions having Tn-elements inserted into the chromosome were obtained as a result of conjugal transfer of vector plasmids carrying the different transposons (Tn9, Tn10, Tn601, Tn5-Mob) and of the consequent isolation of plasmid-free clones of Vibrio cholerae non OI. Identification of auxotrophic mutations induced by the transposons inserted into the bacterial genome made possible the construction of the primary chromosomal map of Vibrio cholerae non OI. The efficient donor strains of Vibrio cholerae non OI were constructed by introducing the transposon Tn5-Mob and the helper plasmid RP-4. The donors are capable of oriented conjugal transfer of chromosome.
Zhuravleva
E A
EA
Smirnova
N I
NI
D000595:Amino Acid Sequence; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005819:Genetic Markers; D008969:Molecular Sequence Data; D010957:Plasmids; D014734:Vibrio cholerae / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005819:Genetic Markers
Ispol'zovanie transposonov dliia izucheniia geneticheskoĭ organizatsii Vibrio cholerae ne 0I.