B-lineage phenotype of lymphoblastoid cell lines from patients with X-linked agammaglobulinemia.
Epstein-Barr virus (EBV)-induced precursor B-cell lines were established from bone marrow cells of patients with X-linked agammaglobulinemia (X-LA). Using seven B-lineage specific monoclonal antibodies marker profiles of these cell lines were examined in order to know developmental stage specific and/or X-LA specific changes of B-cell related cell-surface antigens. CD19, CD20, CR2 (CD21), Fc epsilon receptor (CD23) and HLA-DR antigens were consistently found on the X-LA derived precursor B-cell lines as well as mature lymphoblastoid cell lines from healthy adults. These results suggest that immortalisation of B-cells with EBV is always accompanied by expression of pan-B cell markers and B-cell activation antigen (Fc epsilon receptor) even though the cell lines have precursor B-cell phenotypes as defined by immunoglobulin expression.
Tsuchiya
S
S
Department of Pediatrics, Tohoku University, Sendai.
Fujie
H
H
Konno
T
T
D000328:Adult; D000361:Agammaglobulinemia / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D002461:Cell Line, Transformed; D005434:Flow Cytometry; D008040:Genetic Linkage; D006684:HLA-DR Antigens / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D008297:Male; D010641:Phenotype; D014960:X Chromosome
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D006684:HLA-DR Antigens