Electrochemical determination of azidothymidine in human whole blood.
An electrochemical method based on differential pulse voltammetry is presented for the determination of AZT in whole blood of fasted subjects. A protein-free supernatant of whole blood is prepared using HClO4 precipitation followed by neutralization with phosphate buffer. The AZT is reduced at a hanging mercury drop electrode. The linear dynamic range of standards in buffer is from the detection limit of 4.1 nM to 206.5 microM (1.1 to 55,200 ng/ml). However, in spiked blood samples the linear dynamic range is from 0.029 to 0.29 microM (7.75 to 77.5 ng/ml). The whole blood assay yields a recovery of 92.30 +/- 5.92% compared to the standard solution assay. After a 30-min preparation time, each sample can be analyzed in 10 min by a manual procedure.
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Sharon Drive Corporation, Westlake, Ohio 44145.
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Halsall
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Heineman
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D011232:Chemical Precipitation; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D010084:Oxidation-Reduction; D010472:Perchlorates; D012680:Sensitivity and Specificity; D015215:Zidovudine / Q000097:blood*
D010472:Perchlorates; D015215:Zidovudine