title: Electron transfer proteins of the purple phototrophic bacterium, Rhodopseudomonas rutila.
abstract: The soluble electron transfer protein content of Rhodopseudomonas rutila was found to consist of two basic cytochromes and a (4Fe-4S) ferredoxin. Cytochrome c' was easily identified by its characteristic high spin absorption spectra. The native molecular weight is 29,000 and the subunit is 14,000. Cytochrome c-550 has low spin absorption spectra and a high redox potential (376 mV) typical of cytochromes c2. The molecular weight is about 14,000. The ferredoxin is apparently a dimer (43,000) of approximately 18,000 Da subunits. There are 1.3 to 1.5 iron-sulfur clusters per monomer of 18- to 21-kDa protein. The N-terminal amino acid sequence is like the (7Fe-8S) ferredoxins of Rhodobacter capsulatus and Azotobacter vinelandii. Remarkably, there are only 2 or 3 out of 25 amino acid substitutions. Difference absorption spectra of Rps. rutila membranes indicate that there is not tetraheme reaction center cytochrome c, such as is characteristic of Rps. viridis. However, there are a high potential cytochrome c and a low potential cytochrome b in the membrane, which are suggestive of a cytochrome bc1 complex. Rps. rutila is most similar to Rps. palustris in microbiological properties, yet it does not have the cytochromes c-556, c-554, and c-551 in addition to c2 and c', which are characteristic of Rps. palustris. Furthermore, the Rps. rutila cytochrome c' is dimeric, whereas the same protein from Rps. palustris is the only one known to be monomeric. The cytochrome pattern is more like that of Rhodospirillum rubrum and Rb. capsulatus, which are apparently only able to make cytochromes c2 and c'.
authors.affiliation: Department of Biochemistry, University of Arizona, Tucson 85721.
authors.forename: T E
authors.initials: TE
authors.lastname: Meyer
authors.forename: J
authors.initials: J
authors.lastname: Fitch
authors.forename: G
authors.initials: G
authors.lastname: Van Driessche
authors.forename: J
authors.initials: J
authors.lastname: Van Beeumen
authors.forename: U
authors.initials: U
authors.lastname: Fischer
authors.forename: R G
authors.initials: RG
authors.lastname: Bartsch
authors.forename: M A
authors.initials: MA
authors.lastname: Cusanovich
mesh_terms: D003574:Cytochrome c Group / Q000302:isolation & purification / Q000378:metabolism*; D004579:Electron Transport; D005288:Ferredoxins / Q000302:isolation & purification / Q000378:metabolism*; D046911:Macromolecular Substances; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010788:Photosynthesis; D012241:Rhodopseudomonas / Q000378:metabolism*; D013045:Species Specificity; D013053:Spectrophotometry
chemical_list: D003574:Cytochrome c Group; D005288:Ferredoxins; D046911:Macromolecular Substances