DNA adducts in humans as dosimeters of exposure to environmental, occupational, or dietary genotoxins.
The quantitation of adducts of genotoxins with DNA is probably one of the best indicators of genetic damage due to exposure to toxins or carcinogens. It is generally believed that such adducts can lead to mutations, which in turn can trigger the initiation of the carcinogenic process. DNA adducts have been quantitated in white blood cells and in various tissues of smokers, persons in certain high-exposure occupations, and persons consuming foods contaminated with certain carcinogens. The feasibility of this approach for biochemical epidemiologic studies has been demonstrated using methods such as 32P-postlabeling, enzyme-linked immunosorbent assay, and synchronous fluorescence spectrophotometry. Relatively large interindividual differences in DNA adducts have been observed in both exposed and nonexposed persons. As a result, there are only a few studies in which clear quantitative and qualitative differences between these two groups have been observed. In addition, it appears that in some studies the 32P-postlabeling method does not detect the presence of the polycyclic aromatic hydrocarbon DNA adducts that are detectable by immunoassays. More extensive studies in additional populations at risk should shed further light on the utility of DNA adduct analysis in biochemical monitoring, especially if further refinements in methodology would result in increased sensitivity and specificity.
Schut
H A
HA
Department of Pathology, Medical College of Ohio, Toledo 43699.
Shiverick
K T
KT
D000818:Animals; D004247:DNA / Q000378:metabolism*; D004032:Diet; D004781:Environmental Exposure; D006801:Humans; D009153:Mutagens / Q000378:metabolism*; D016273:Occupational Exposure; D012907:Smoking / Q000378:metabolism
D009153:Mutagens; D004247:DNA