cDNA cloning of the beta-subunit of the human gastric H,K-ATPase.
A full-length cDNA clone encoding the human gastric H,K-ATPase (EC 3.6.1.36)beta-subunit was isolated from a human gastric mucosal lambda gt10 library using oligonucleotide probes which were based on the cDNA sequence from rat and rabbit H,K-ATPase beta-subunits. The insert was 1407 bp in length and encoded a polypeptide of 291 amino acids with a MW = 33,367 Da. It exhibited 84.2%, 85.6% and 81.3% identity to the H,K-ATPase beta-subunits of rabbit, pig and rat, respectively.
Ma
J Y
JY
Department of Medical and Physiological Chemistry, Biomedical Centre, Uppsala, Sweden.
Song
Y H
YH
Sjöstrand
S E
SE
Rask
L
L
Mårdh
S
S
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005753:Gastric Mucosa / Q000201:enzymology*; D017506:H(+)-K(+)-Exchanging ATPase; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D011817:Rabbits; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D015345:Oligonucleotide Probes; D004247:DNA; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase