title: Isolation of monoaminergic synaptosomes from rat brain by immunomagnetophoresis.
abstract: Monoaminergic synaptosomes have been isolated and purified from rat brain by immunomagnetophoresis. This novel technique uses magnetic beads to which Protein A is bound. Noradrenergic, dopaminergic, and serotonergic synaptosomes (previously cell-surface labelled with anti-dopamine-beta-hydroxylase, anti-tyrosine hydroxylase, and anti-tryptophan hydroxylase, respectively) may be isolated in a highly purified state. The synaptosomal subpopulations are recovered in a viable metabolic state and show glucose-stimulated respiration and Ca2(+)-dependent neurotransmitter release. A novel subtype of dopamine-beta-hydroxylase was found in dopaminergic terminals. No evidence for glutamate corelease from monoaminergic synaptosomes was obtained.
authors.affiliation: Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, England.
authors.forename: M
authors.initials: M
authors.lastname: Docherty
authors.forename: H F
authors.initials: HF
authors.lastname: Bradford
authors.forename: C D
authors.initials: CD
authors.lastname: Cash
authors.forename: M
authors.initials: M
authors.lastname: Ehret
authors.forename: M
authors.initials: M
authors.lastname: Maitre
authors.forename: T H
authors.initials: TH
authors.lastname: Joh
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism*; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure*; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007158:Immunologic Techniques; D008280:Magnetics / Q000379:methods; D018377:Neurotransmitter Agents / Q000378:metabolism; D010101:Oxygen Consumption; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism / Q000648:ultrastructure*; D014365:Tryptophan Hydroxylase / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D001679:Biogenic Amines; D005904:Glial Fibrillary Acidic Protein; D018377:Neurotransmitter Agents; D014446:Tyrosine 3-Monooxygenase; D014365:Tryptophan Hydroxylase; D004299:Dopamine beta-Hydroxylase; D011188:Potassium