title: Experimental glaucoma significantly decreases atrial natriuretic factor (ANF) receptors in the ciliary processes of the rabbit eye.
abstract: ANF binding sites were analysed in the ciliary processes of rabbits with unilateral experimental glaucoma which had been induced by injecting alpha-chymotrypsin into the posterior chamber of the right eyes. The intraocular pressure (IOP) of glaucomatous eyes was significantly greater (28.4 +/- 4 mmHg) than that of normotensive control eyes (13.1 +/- 1.4 mmHg, mean +/- S.E.M., n = 23, P less than 0.05). ANF concentrations in aqueous humour and the ciliary processes were significantly higher in glaucomatous eyes (91 +/- 2 pg ml-1 and 30.4 +/- 4.2 pg g-1 wet weight) than in normal eyes (3.1 +/- 2.2 pg ml-1 and 10.2 +/- 2.7 pg g-1 wet weight, respectively, n = 6, P less than 0.01). The number of ANF-binding sites (Bmax) in the ciliary processes of glaucomatous rabbit eyes was significantly decreased in comparison to the controls (24 +/- 4 vs. 13 +/- 3 fmol mg-1 protein, n = 10, P less than 0.05). These data suggest that ANF receptors in the ciliary processes are down-regulated and that ANF may play an important role in the pathophysiology of experimental glaucoma.
authors.affiliation: Departamento de Medicina Interna III, Ocular Hospital Universitario de San Carlos, Ciudad Universitaria, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Fernandez-Durango
authors.forename: J M
authors.initials: JM
authors.lastname: Ramirez
authors.forename: A
authors.initials: A
authors.lastname: Trivino
authors.forename: D
authors.initials: D
authors.lastname: Sanchez
authors.forename: P
authors.initials: P
authors.lastname: Paraiso
authors.forename: M
authors.initials: M
authors.lastname: Garcia De Lacoba
authors.forename: A
authors.initials: A
authors.lastname: Ramirez
authors.forename: J J
authors.initials: JJ
authors.lastname: Salazar
authors.forename: A
authors.initials: A
authors.lastname: Fernandez-Cruz
authors.forename: J
authors.initials: J
authors.lastname: Gutkowska
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001665:Binding Sites; D002918:Chymotrypsin; D002924:Ciliary Body / Q000737:chemistry*; D005901:Glaucoma / Q000139:chemically induced / Q000378:metabolism*; D007429:Intraocular Pressure / Q000502:physiology; D011817:Rabbits; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000032:analysis*
chemical_list: D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D002918:Chymotrypsin; D017461:Receptors, Atrial Natriuretic Factor