title: Involvement of the Chox-4 chicken homeobox genes in determination of anteroposterior axial polarity during limb development.
abstract: We have isolated and identified four chicken homeobox genes in the upstream region of the Chox-4 complex. The Chox-4g and -4f genes, at the 5' extremity of the complex, were expressed locally in the vicinity of the zone of polarizing activity (ZPA) at early stages of limb development, substantiating the involvement of the genes in anteroposterior axis determination. To confirm their function, we implanted a bead containing retinoic acid, or the ZPA itself, in the anterior margin of the limb bud, leading to formation of mirror-image duplicated digits, and observed the resultant change in gene expression. Expression of the Chox-4g and -4f genes was induced in the new digit-forming region. Those results suggest that positional information assigned by a ZPA morphogen is imprinted on cellular memory by expression of the Chox-4 genes to maintain positional signaling along the anteroposterior axis in the limb field.
authors.affiliation: Department of Pharmacology, Kawasaki Medical School, Kurashiki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nohno
authors.forename: S
authors.initials: S
authors.lastname: Noji
authors.forename: E
authors.initials: E
authors.lastname: Koyama
authors.forename: K
authors.initials: K
authors.lastname: Ohyama
authors.forename: F
authors.initials: F
authors.lastname: Myokai
authors.forename: A
authors.initials: A
authors.lastname: Kuroiwa
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: S
authors.initials: S
authors.lastname: Taniguchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D003001:Cloning, Molecular; D005121:Extremities / Q000196:embryology*; D015870:Gene Expression / Q000187:drug effects; D005801:Genes, Homeobox / Q000502:physiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D014212:Tretinoin