Effects of acute and chronic ethanol administration on phosphoinositide metabolism in mouse brain.
A radiotracer technique in combination with lithium injection was used to probe the effects of acute and chronic ethanol administration on poly-phosphoinositide turnover in mouse brain. C57Bl/6J mice were injected intracerebrally with 3H-inositol to label the inositol phospholipids and inositol phosphates in brain. Subsequently, the accumulation of labeled inositol monophosphates was assessed 4 hr after i.p. injection of lithium (6 meq/kg body wt). Using this experimental paradigm, results indicated a significant increase in labeled inositol monophosphates in the chronic ethanol group and a decrease in the same compound in animals after acute ethanol intoxication.
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Biochemistry Department, School of Medicine, University of Missouri, Columbia 65212.
Navidi
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Yoa
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Lin
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D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004334:Drug Administration Schedule; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D007294:Inositol / Q000378:metabolism; D008094:Lithium / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism*; D014316:Tritium
D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D014316:Tritium; D000431:Ethanol; D007294:Inositol; D008094:Lithium