[Protective action of biphenyl dimethyl dicarboxylate (DDB) against liver nuclear DNA damage induced by carcinogens].
The protective effect of DDB against carcinogen-induced DNA damage was examined in the present investigation. Preincubation of rat liver nuclei with DDB (1 mmol/L) resulted in inhibition of binding of 3H-benzo (a) pyrene to nuclear DNA. The inhibition rate was about 60%. Unscheduled DNA synthesis (UDS) of freshly isolated rat hepatocytes induced by aflatoxin B1 (10(-7) mol/L) was also dose dependently inhibited by DDB (10(-6)-10(-3) mol/L). Oral administration of DDB at 200 mg/kg once daily for 3 days was effective to induce increase of liver cytosol glutathione-S-transferase and microsomal UDPG-transferase in mice. The results indicate that DDB is able directly or indirectly to antagonize certain carcinogen-induced DNA damages.
Qing
W
W
Liu
G
G
D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D002467:Cell Nucleus; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects*; D004149:Dioxoles / Q000494:pharmacology*; D014453:Glucuronosyltransferase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000201:enzymology; D008297:Male; D051379:Mice; D051381:Rats
D004149:Dioxoles; C041398:7,7'-dimethoxy-(4,4'-bi-1,3-benzodioxole)-5,5'-dicarboxylic acid dimethyl ester; D016604:Aflatoxin B1; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase