Production and characterization of antibodies to ouabain.
Two monoclonal antibodies (249F8 and 278A9) to ouabain were produced by somatic cell fusion. They reacted in dose-dependent manners with ouabain and digoxin. These antibodies were supposed to recognize the partial structure important for cardiac compounds to show their pharmacological activity, Na+, K(+)-ATPase inhibition in a competitive mode against K+. These monoclonal antibodies may be useful for the immunochemical isolation, the structural elucidation, and the quantitative measurement of putative endogenous digoxin-like factors.
Terano
Y
Y
Department of Physiology, Osaka City University Medical School.
Tomii
A
A
Masugi
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D001798:Blood Proteins / Q000032:analysis*; D002298:Cardenolides; D002999:Clone Cells; D003429:Cross Reactions / Q000276:immunology; D004077:Digoxin; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D010042:Ouabain / Q000276:immunology*; D012503:Saponins; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D010042:Ouabain; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase