In situ hybridization for the demonstration of bovine leukemia virus transcripts in lymphosarcoma cells using biotinylated probes.
Bovine leukemia virus (BLV) RNA was demonstrated in peripheral blood lymphocytes treated with concanavalin A or phytohemagglutinin from a BLV-infected ox, and in lymphosarcoma cells cultured without mitogen from an enzootic bovine leukosis cow by in situ hybridization using biotinylated DNA probes.
Okada
K
K
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Japan.
Hosokawa
Y
Y
Aida
Y
Y
Ohshima
K
K
D000818:Animals; D002417:Cattle; D015342:DNA Probes; D016583:Enzootic Bovine Leukosis / Q000382:microbiology*; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D008214:Lymphocytes / Q000382:microbiology*; D008228:Lymphoma, Non-Hodgkin / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D012367:RNA, Viral