Intracellular pH and sodium-proton exchange activity of lymphocytes in stroke-prone spontaneously hypertensive rats.
1. At the age of 20 weeks, intracellular pH (pHi) of circulating lymphocytes suspended in HCO(3-)-free NaCl media was not significantly different between stroke-prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar-Kyoto rats (WKY). 2. The initial recovery rate of pHi in lymphocytes tended to be greater in SHRSP than in WKY after the addition of 60 mmol/L or 120 mmol/L of NaCl, but there was no statistically significant difference. 3. The H+ equivalent efflux rate, which was a true reflection of Na(+)-H(+) activity, was significantly greater in SHRSP than in WKY (P less than 0.05). The difference in H+ equivalent efflux rate was not due to the difference in cellular buffering power between the two groups (P greater than 0.05). An increased Na(+)-H(+) exchange activity may play a partial role in the pathogenesis of hypertension.
Li
D Y
DY
Department of Pediatrics, Shimane Medical University, Izumo, Japan.
Kobayashi
A
A
Nara
Y
Y
Ikeda
K
K
Mori
C
C
Yamori
Y
Y
D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000502:physiology*; D002561:Cerebrovascular Disorders / Q000097:blood / Q000503:physiopathology; D005452:Fluoresceins; D006863:Hydrogen-Ion Concentration; D006973:Hypertension / Q000097:blood / Q000503:physiopathology*; D007424:Intracellular Fluid / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012964:Sodium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers
D002352:Carrier Proteins; D005452:Fluoresceins; D017923:Sodium-Hydrogen Exchangers; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D012964:Sodium