Effects of aging on postsynaptic alpha 1-adrenoceptor mechanisms in rat aorta.
1. Effects of aging on alpha 1-adrenoceptor and S2-serotonin receptor mechanisms in rat aorta were studied. 2. In rat aorta, the potency (pD2 value) of norepinephrine or phenylephrine increased with age from 3 to 10 weeks, but decreased thereafter with age from 10 to 80 weeks. The affinity (pKA value) of norepinephrine or phenylephrine and of prazosin (pA2 value) did not alter with aging. 3. In rat vas deferens, the efficacy of norepinephrine and the maximum binding sites of [3H]prazosin increased with age from 3 to 18 weeks, but decreased thereafter with age from 18 to 60 weeks. The affinity (pKA value) of norepinephrine and the dissociation constant (KD value) of prazosin did not alter with aging. 4. In rat aorta, the potency (pD2 value) and affinity (pKA value) of serotonin, and affinity (pA2 value) of ketanserin did not alter with aging. 5. There is no significant difference between slopes of regression lines between a cytosolic free Ca2+ level [( Ca2+]i) and tension in the presence of phenylephrine in aorta strips from 10- and 60-week-old rats. 6. These results suggest that changes in alpha 1-adrenoceptor mechanisms with aging are due to changes in receptor density or receptor reserve, but not to changes in affinity of drugs to alpha 1-adrenoceptor or sensitivity of contractile system to Ca2+ mediated through alpha 1-adrenoceptor, and that S2-serotonin receptor mechanisms in rat aorta do not alter with aging.
Takayanagi
I
I
Department of Chemical Pharmacology, Toho University School of Pharmaceutical Sciences, Chiba, Japan.
Koike
K
K
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism* / Q000502:physiology
D011942:Receptors, Adrenergic, alpha