Selenium: inhibition of microtubule formation and interaction with tubulin.
We have studied the interaction of Na2SeO3 with microtubule proteins and tubulin. This selenium compound inhibits the polymerization of MTP (half-inhibition occurred for Na2SeO3 10 microM), and to a lesser that of tubulin. This effect of selenite is related to the formation of disulfide bridges between tubulin sulfhydryl groups, inducing a conformational change of the protein. This is corroborated by the modified binding of colchicine and vinblastine in presence of selenium. The selenite inhibitory concentrations are similar to the toxic blood levels of selenium (40 microM).
Leynadier
D
D
Laboratoire de Physique Pharmaceutique, Faculté de Pharmacie, Marseille, France.
Peyrot
V
V
Codaccioni
F
F
Briand
C
C
D000818:Animals; D003078:Colchicine / Q000378:metabolism; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008868:Microtubule Proteins / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D011108:Polymers; D012643:Selenium / Q000494:pharmacology*; D018038:Sodium Selenite; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013552:Swine; D013696:Temperature; D014404:Tubulin / Q000378:metabolism*; D014747:Vinblastine / Q000494:pharmacology
D046911:Macromolecular Substances; D008868:Microtubule Proteins; D011108:Polymers; D013438:Sulfhydryl Compounds; D014404:Tubulin; D014747:Vinblastine; D012643:Selenium; D018038:Sodium Selenite; D003078:Colchicine