title: Cyclic adenosine monophosphate-phosphodiesterase activity in cultured keratinocytes from patients with atopic eczema.
abstract: A number of previous studies have documented an increase in cyclic adenosine monophosphate-phosphodiesterase activity in patients with atopic dermatitis. We have studied the activity of this enzyme in keratinocytes cultured from patients with atopic dermatitis compared to keratinocytes from non-atopic controls. Results show that these pure preparations of keratinocytes express only one form of cyclic adenosine monophosphate phosphodiesterase with a Km of 1.9 microM, and a Vmax of 17 pmol/min/mg of substrate converted. We conclude that cyclic adenosine monophosphate-phosphodiesterase activity in cultured keratinocytes from patients with atopic eczema does not differ from controls.
authors.affiliation: Department of Experimental Dermatology, London Hospital, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Wright
authors.forename: H
authors.initials: H
authors.lastname: Navsaria
authors.forename: I M
authors.initials: IM
authors.lastname: Leigh
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D002478:Cells, Cultured; D003876:Dermatitis, Atopic / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology* / Q000473:pathology
chemical_list: D015105:3',5'-Cyclic-AMP Phosphodiesterases