Binding of a histidine-rich peptide to Porphyromonas gingivalis.
Porphyromonas gingivalis 381 cells were incubated with 125I-histidine-rich polypeptide (histatin) 5 in the presence or absence of unlabeled histatin 5, to evaluate the histatin-binding capacity of the cells. The binding of histatin 5 was rapid, reversible, saturable and specific. The number of histatin 5-binding sites per cell was 3,600, and the dissociation constant (Kd) was in the order of 10(-6) M. These findings suggest that histatin interacts with certain bacterial cells through specific binding sites on their surface, and will allow the development of a histatin radioreceptor assay.
Murakami
Y
Y
Department of Preventive Dentistry, Faculty of Dentistry, Osaka University, Japan.
Shizukuishi
S
S
Tsunemitsu
A
A
Nakashima
K
K
Kato
Y
Y
Aimoto
S
S
D001667:Binding, Competitive; D054808:Histatins; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000378:metabolism*
C068075:HTN3 protein, human; D054808:Histatins; D008565:Membrane Proteins; D012471:Salivary Proteins and Peptides