Isolation of a phosphatidylserine transfer protein from yeast cytosol.
A phospholipid transfer protein with a broad substrate specificity was isolated from yeast cytosol. The rate of transfer catalyzed by this protein in vitro is highest for phosphatidylserine; phosphatidylethanolamine, cardiolipin, phosphatidic acid and ergosterol are transported at a lower rate. In contrast to the yeast phosphatidylinositol transfer protein (Daum, G. and Paltauf, F. (1984) Biochim. Biophys. Acta 794, 385-391) the phosphatidylserine transfer protein does not catalyze the translocation of phosphatidylinositol or phosphatidylcholine. Using chromatographic methods the phosphatidylserine transfer protein was enriched approximately 3000-fold over yeast cytosol. The protein is inactivated by heat, detergents and proteinases. Divalent cations strongly inhibit the transfer of phosphatidylserine in vitro, and EDTA at low concentrations has a stimulatory effect.
Lafer
G
G
Institut für Biochemie und Lebensmittelchemie, Technische Universität Graz, Austria.
Szolderits
G
G
Paltauf
F
F
Daum
G
G
D000327:Adsorption; D000645:Ammonium Sulfate; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002384:Catalysis; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003600:Cytosol / Q000737:chemistry*; D017886:Durapatite; D006882:Hydroxyapatites; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification*; D010718:Phosphatidylserines / Q000378:metabolism*; D049294:Phospholipid Transfer Proteins; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000187:drug effects; D013379:Substrate Specificity
D002352:Carrier Proteins; D006882:Hydroxyapatites; D008565:Membrane Proteins; D010718:Phosphatidylserines; D049294:Phospholipid Transfer Proteins; D017886:Durapatite; D000645:Ammonium Sulfate