title: Repair of UV-induced pyrimidine dimers in the individual genes Gart, Notch and white from Drosophila melanogaster cell lines.
abstract: The excision repair of UV-induced pyrimidine dimers was investigated in three genes: Gart, Notch and white in a permanent Drosophila cell line Kc, derived from wild type Drosophila melanogaster embryonic cells. In this cell line Gart and Notch are actively transcribed, whereas white is not expressed. In all three genes UV-induced pyrimidine dimers were removed with the same rate and to the same extent: 60% removal within 16 hours, up to 80-100% in 24 hours after irradiation with 10 or 15 J/m2 UV. These kinetics are similar to the time course of dimer removal measured in the genome overall. No difference in repair of the inactive white locus compared to the active Gart and Notch genes was found. Similar results were obtained using a different wild type cell line, SL2, although repair appeared to be somewhat slower in this cell line. The results are discussed with respect to the data found for gene specific repair in other eukaryotic systems.
authors.affiliation: MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
authors.forename: J G
authors.initials: JG
authors.lastname: de Cock
authors.forename: E C
authors.initials: EC
authors.lastname: Klink
authors.forename: W
authors.initials: W
authors.lastname: Ferro
authors.forename: P H
authors.initials: PH
authors.lastname: Lohman
authors.forename: J C
authors.initials: JC
authors.lastname: Eeken
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D004247:DNA / Q000528:radiation effects; D004260:DNA Repair; D004331:Drosophila melanogaster; D004720:Endonucleases / Q000378:metabolism; D007700:Kinetics; D011740:Pyrimidine Dimers; D015183:Restriction Mapping; D013604:T-Phages / Q000201:enzymology; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
chemical_list: D011740:Pyrimidine Dimers; D004247:DNA; D004720:Endonucleases