Immunohistological diagnosis of rabbit haemorrhagic disease in experimentally infected rabbits in Spain.
The immunoreactivity of routinely processed liver and lung tissue samples obtained from rabbits inoculated with tissue explants from naturally infected animals when antisera directed against parvovirus from different species (canine, feline and porcine) as well as a RHD virus antiserum were employed has been tested by different immunoperoxidase methods. Cross-reactivity between RHD-virus antigens and parvovirus antigens was present. Best results were obtained with RHD and canine parvovirus antisera with the ABC method. The immunoreactivity in the liver was found in hepatocytes, Kupffer and bile duct cells. In the lung, it was exclusively observed in intravascular macrophages.
Carrasco
L
L
Department of Anatomy and Comparative Pathology, Faculty of Veterinary, University of Córdoba, Spain.
Rodriguez
F
F
Martin de las Mulas
J
J
Wohlsein
P
P
Fernandez
A
A
D000818:Animals; D003429:Cross Reactions; D006470:Hemorrhage / Q000175:diagnosis / Q000662:veterinary*; D007150:Immunohistochemistry; D010321:Parvoviridae / Q000276:immunology*; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D011817:Rabbits