Outer capsid glycoprotein vp7 is recognized by cross-reactive, rotavirus-specific, cytotoxic T lymphocytes.
Cytotoxic T lymphocytes (CTLs) generated in mice orally inoculated with rotaviruses lyse target cells infected with different rotavirus serotypes (cross-reactive CTLs). Using vaccinia virus recombinants expressing individual rotavirus proteins from two different rotavirus serotypes, we found that cross-reactive CTLs recognize target cells expressing outer capsid protein vp7 better than those expressing outer capsid protein vp4 or inner capsid protein vp6. These findings may be relevant to vaccine strategies which include immunization with reassortant rotaviruses or viral or bacterial vectors expressing individual rotavirus proteins. The region or regions of vp7 which are antigenically conserved among different rotavirus serotypes and recognized by cross-reactive CTLs remain to be determined.
Offit
P A
PA
Division of Infectious Diseases, Children's Hospital of Philadelphia, Pennsylvania 19104.
Boyle
D B
DB
Both
G W
GW
Hill
N L
NL
Svoboda
Y M
YM
Cunningham
S L
SL
Jenkins
R J
RJ
McCrae
M A
MA
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012401:Rotavirus / Q000276:immunology*; D012703:Serotyping; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000956:Antigens, Viral; D036022:Capsid Proteins; D008562:Membrane Glycoproteins; C050779:VP7 protein, Rotavirus