Restriction endonuclease digest patterns of chromosomal DNA from group B beta-haemolytic streptococci.
Scanning densitometry and computer-assisted numerical analysis were used to examine restriction endonuclease digest patterns (RDPs) of chromosomal DNA from 26 infecting strains and 44 vaginal isolates of group B beta-haemolytic streptococci (GBS). At the 95% similarity level, HindIII RDPs of serotype Ia and III strains clustered into four and three RDP types, respectively. Nine of 10 strains from neonates with early-onset septicaemia belonged to two particular RDP types (Ia-3 and III-3). In contrast, serotype III GBS strains from meningitis cases were not characterised by particular RDP types. Associations between RDPs and certain phenotypic characteristics were also found.
Nagano
Y
Y
Department of Microbiology, School of Hygienic Sciences, Kitasato University, Sagamihara, Japan.
Nagano
N
N
Takahashi
S
S
Murono
K
K
Fujita
K
K
Taguchi
F
F
Okuwaki
Y
Y
D002876:Chromosomes, Bacterial / Q000737:chemistry; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D003720:Densitometry; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016920:Meningitis, Bacterial / Q000382:microbiology; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D015203:Reproducibility of Results; D018805:Sepsis / Q000145:classification / Q000382:microbiology; D013290:Streptococcal Infections / Q000145:classification / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000145:classification / Q000235:genetics*; D014621:Vagina / Q000382:microbiology
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes