[Detection of human papillomavirus (HPV) type 16 DNA relative sequence in the tissue of laryngeal carcinoma].
By using alpha 32-P-dCTP labeled Chinese Human papillomavirus type 16 DNA (CHPV 16 DNA) probe, we have adopted Southern blot method and detected HPV subtype 16 DNA (1/6) and HPV 16 DNA relative sequence (3/6) in 6 biopsy specimens of laryngeal carcinoma. The result showed a severe infection of HPV in laryngeal carcinoma. This indicates that HPV infection may play a pathogenetic role in the cancer.
Wang
H
H
Shanxi Provincial Cancer Institude, Tiyuan.
D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000382:microbiology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000382:microbiology*; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid; D014412:Tumor Virus Infections / Q000382:microbiology
D015342:DNA Probes; D004279:DNA, Viral