Fitting progress curves in assays of slow-binding enzyme inhibitors.
Practical aspects of fitting progress curves deriving from assays of slow-binding enzyme inhibitors in order to extract characteristic parameters is briefly discussed. An approach little-used, but which may provide more accurate estimates, is the simultaneous analysis of two assays differing from one another in the order of addition of reactants.
Giroux
E
E
Marion Merrell Dow Research Institute, Cincinnati, Ohio 45215.
D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D019272:Leukocyte Elastase; D008433:Mathematics; D008962:Models, Theoretical; D009842:Oligopeptides / Q000494:pharmacology*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000097:blood
D004791:Enzyme Inhibitors; D009842:Oligopeptides; C084918:MDL 27013; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase