title: The role of mannose in the receptor of the monocyte locomotion inhibitory factor produced by Entamoeba histolytica.
abstract: The effect of the monocyte locomotion inhibitory factor (MLIF) produced by Entamoeba histolytica is diminished, if not cancelled, when human monocytes are pre-exposed to concanavalin A or sodium periodate, respectively, but not when MLIF is pretreated with sodium periodate. When the MLIF-inhibited monocyte locomotion assays were performed in the presence of 12 different carbohydrates, only the runs containing D-mannose, 4-O-beta-galactosyl-mannoside or mannan revealed a significant reduction in the inhibitory effect. Finally, the MLIF activity was virtually absorbed out with mannan-coupled Sepharose 4B beads. This suggests that D-mannose constitutes an essential part of the receptor for MLIF on the human monocyte membrane.
authors.affiliation: Division of Immunology, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City.
authors.forename: R R
authors.initials: RR
authors.lastname: Kretschmer
authors.forename: E M
authors.initials: EM
authors.lastname: Castro
authors.forename: G
authors.initials: G
authors.lastname: Pacheco
authors.forename: G
authors.initials: G
authors.lastname: Rico
authors.forename: O
authors.initials: O
authors.lastname: Díaz-Guerra
authors.forename: J
authors.initials: J
authors.lastname: Arellano
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000378:metabolism* / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology; D004748:Entamoeba histolytica / Q000378:metabolism*; D006801:Humans; D008358:Mannose / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology / Q000187:drug effects*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology
chemical_list: D001685:Biological Factors; D010504:Periodic Acid; D003208:Concanavalin A; D009240:N-Formylmethionine Leucyl-Phenylalanine; C009288:metaperiodate; D008358:Mannose