A monoclonal antibody (SZ-53) against thrombomodulin inhibits thrombin-mediated release of t-PA and PGI2 from endothelial cells.
Monoclonal antibodies against thrombomodulin have become a useful means to study the structure and function of thrombomodulin. In this study, we used a monoclonal antibody against human thrombomodulin, named SZ-53, to investigate the function of thrombomodulin on the surface of cultured human umbilical vein endothelial cells. Preincubation of endothelial cells with SZ-53 before addition of thrombin not only inhibited thrombomodulin mediated activation of protein C, but also inhibited thrombin mediated release of t-PA and PGI2 from endothelial cells. The inhibitory effects depended on the concentration of SZ-53 IgG. According to our experimental results, we suggest that thrombomodulin on the surface of endothelial cells could participate in the regulation of thrombin mediated release of t-PA and PGI2 from these cells.
Zhou
Q
Q
Jiangsu Institute of Hematology, Suzhou Medical College, Peoples' Republic of China.
Zhao
Y
Y
Li
P
P
Bai
X
X
Ruan
C
C
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D011956:Receptors, Cell Surface / Q000276:immunology*; D018179:Receptors, Thrombin; D013917:Thrombin / Q000037:antagonists & inhibitors*; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014471:Umbilical Veins
D000911:Antibodies, Monoclonal; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; D011464:Epoprostenol; D013917:Thrombin; D010959:Tissue Plasminogen Activator