The stem cells of a primordial germ cell-derived teratocarcinoma have the ability to form viable mouse chimeras.
A euploid testicular teratocarcinoma line, STT-3, has been established from a tumor spontaneously occurring in the testis of a 129/Sv-ter male. Developmental ability of the STT-3 stem cells was tested by injecting these cells into mouse blastocysts. The frequency and the extent of chimerism were examined in mid-gestational fetuses and in live-born mice. STT-3 stem cells form viable chimeras at a high rate and differentiate into normal tissues. This is the first reported testicular teratocarcinoma-derived stem line with a proven capacity to form viable chimeric mice upon injection into the blastocysts.
Hanaoka
K
K
Laboratory of Mammalian Developmental Biology, Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
Hayasaka
M
M
Noguchi
T
T
Kato
Y
Y
D000818:Animals; D001755:Blastocyst / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D002678:Chimera; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation / Q000473:pathology; D009373:Neoplasms, Germ Cell and Embryonal / Q000473:pathology*; D013234:Stem Cells / Q000473:pathology*; D013724:Teratoma / Q000473:pathology*; D013736:Testicular Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000473:pathology