Malonyl-CoA in skeletal muscle and liver of streptozotocin-diabetic rats.
Malonyl-CoA, the inhibitor of carnitine palmitoyl transferase I, has been examined in this study in the muscle and liver of diabetic rats. Male Sprague-Dawley rats were rendered diabetic with streptozotocin (6 mg/100 g body wt). The gastrocnemius/plantaris muscles and liver samples were frozen at liquid nitrogen temperature. Muscle malonyl-CoA was 1.8 +/- 0.2 pmol/mg in control rats and 1.5 +/- 0.2 pmol/mg in the diabetic rats. This difference was not statistically significant. Liver malonyl-CoA of control rats was 8.6 +/- 0.8 pmol/mg, in comparison to 4.3 +/- 0.6 pmol/mg in diabetic rats. In the liver, high concentrations of malonyl-CoA inhibit fatty acid oxidation and ketogenesis. Failure of malonyl-CoA to decline in muscle in the diabetic may be responsible in part for the diversion of fatty acids to the liver, thereby enhancing hepatic fatty acid oxidation and ketogenesis.
Elayan
I M
IM
Department of Zoology, Brigham Young University, Provo, Utah 84602.
Cartmill
D C
DC
Eckersell
C B
CB
Wilkin
J
J
Winder
W W
WW
D020155:3-Hydroxybutyric Acid; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D000242:Cyclic AMP / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005635:Fructosediphosphates / Q000097:blood; D006003:Glycogen / Q000097:blood; D006885:Hydroxybutyrates / Q000097:blood; D008099:Liver / Q000378:metabolism*; D008297:Male; D008316:Malonyl Coenzyme A / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013311:Streptozocin
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D005635:Fructosediphosphates; D006885:Hydroxybutyrates; D008316:Malonyl Coenzyme A; D013311:Streptozocin; C027652:fructose 2,6-diphosphate; D006003:Glycogen; D000242:Cyclic AMP; D020155:3-Hydroxybutyric Acid