Increased sensitivity of a rotavirus serotyping enzyme-linked immunosorbent assay by the incorporation of CaCl2.
The sensitivity of a rotavirus serotyping enzyme-linked immunosorbent assay (ELISA) was improved by the addition of 0.5 mM CaCl2 to the washing buffer and reagent diluent. Twenty-nine of 63 (46%) previously untyped bovine and equine faecal rotavirus samples were serotyped in the modified assay. A differential response to Ca2+ ions was noted for different G-serotypes suggesting that serotyping assays performed without the inclusion of CaCl2 in the assay buffers may produce biased results.
Fitzgerald
T A
TA
Moredun Research Institute, Edinburgh, UK.
Browning
G F
GF
D000818:Animals; D002021:Buffers; D002122:Calcium Chloride; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000382:microbiology; D012401:Rotavirus / Q000145:classification*; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods*
D002021:Buffers; D002122:Calcium Chloride