Uptake of inorganic pyrophosphate by Bacillus megaterium.
Cells of Bacillus megaterium take up inorganic pyrophosphate, employing a saturable carrier which is sensitive to sulfhydryl reagents, orthophosphate, and arsenate. Uptake is stimulated by proton ionophores, including CCCP and nigericin, indicating that proton cotransport can lead to an opposing gradient. Inhibitor sensitivity, as well a relatively high Km for inorganic pyrophosphate render it likely that uptake is mediated by an orthophosphate transport system.
Villiotte
P J
PJ
Committee on Biochemistry, Bowdoin College, Brunswick, ME 04011.
Riefsnyder
P C
PC
Bloomstone
J A
JA
Howland
J L
JL
D001410:Bacillus megaterium / Q000378:metabolism*; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000378:metabolism; D011756:Diphosphates / Q000378:metabolism*
D002352:Carrier Proteins; D011756:Diphosphates