title: Presence of human papillomavirus genome in human tumor cell lines and cultured cells.
abstract: A series of 47 human carcinoma cell lines and their cultured cells were examined for human papillomavirus (HPV) genomes with the use of an HPV detection kit (DNA-RNA hybridization, mixed HPV DNA probe of types 6, 11, 16, 18, 31, 33 and 35). Four of 8 cases of mild dysplasia, 3 of 9 cases of severe dysplasia, 3 of 7 cases of carcinoma in situ, 3 of 15 cases of uterine carcinoma and 5 of 6 cases of condyloma acuminatum were shown to contain the HPV DNA genome in primary cultured cells, while HPV was not detected in the third-passage cells except for the three cases of large cell, nonkeratinizing squamous cell carcinoma. HPV was also not detected in such normal tissues as uterine cervical squamous epithelium, uterine cervical columnar epithelium and endometrium. The presence of HPV DNA genomes was detected consistently in the passages of three lines (SKG-II, HKMUS and HKTUS; large cell nonkeratinizing squamous cell carcinomas of the uterine cervix) with the use of the Southern Blot method (DNA-DNA hybridization, mixed HPV probe of types 6, 11, 16 and 18). HPV type 16 DNA was detected in HKTUS, and HPV type 18 DNA was found in SKG-II and HKMUS. The other 44 cell lines, including ovarian carcinoma, endometrial carcinoma, sarcoma, gastric cancer, pancreatic cancer and rectal cancer, were negative for the HPV-6, HPV-11, HPV-16, HPV-18, HPV-31, HPV-33 and HPV-35 genomes under stringent hybridization conditions.
authors.affiliation: Ishiwata Obstetrics and Gynecologic Hospital, Ibaraki-ken, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Ishiwata
authors.forename: C
authors.initials: C
authors.lastname: Ishiwata
authors.forename: M
authors.initials: M
authors.lastname: Soma
authors.forename: H
authors.initials: H
authors.lastname: Akagi
authors.forename: H
authors.initials: H
authors.lastname: Hiyama
authors.forename: T
authors.initials: T
authors.lastname: Nakaguchi
authors.forename: I
authors.initials: I
authors.lastname: Ono
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology; D002294:Carcinoma, Squamous Cell / Q000382:microbiology; D002460:Cell Line / Q000382:microbiology; D003218:Condylomata Acuminata / Q000382:microbiology; D015342:DNA Probes; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D005833:Genital Neoplasms, Female / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000382:microbiology; D014412:Tumor Virus Infections / Q000235:genetics*; D014594:Uterine Neoplasms / Q000382:microbiology
chemical_list: D015342:DNA Probes; D004279:DNA, Viral