In vitro antiviral activity of polyoxotungstate (PM-19) and other polyoxometalates against herpes simplex virus.
Polyoxotungstates with Keggin-type structure were found to demonstrate marked antiherpetic activity. K7[TiW10PO40].6H2O (PM-19) caused a decrease in plaque formation by several strains of herpes simplex virus (HSV) type 1, including acyclovir-resistant (thymidine kinase-negative) strains, at concentrations which were not toxic to the host cells. The 50% plaque-inhibiting concentration (EC50) for the different strains was between 20 and 50 micrograms/ml. Single-cycle HSV growth was also inhibited by PM-19. PM-19 inhibited viral DNA synthesis in HSV-infected cells at a concentration of 5 micrograms/ml but did not exhibit a virucidal effect, and pretreatment of the host cells with PM-19 did not provide resistance to herpes infection. Yet, virus adsorption to the cells was markedly affected at PM-19 concentrations higher than 25 micrograms/ml. PM-19 was also effective against human cytomegalovirus, but not against adenoviruses and varicella-zoster virus, although it did delay the development of the cytopathic effect of these viruses.
Fukuma
M
M
Division of Chemotherapy, School of Medicine, Keio University, Tokyo, Japan.
Seto
Y
Y
Yamase
T
T
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004279:DNA, Viral / Q000032:analysis; D006561:Herpes Simplex / Q000188:drug therapy*; D006801:Humans; D011108:Polymers / Q000627:therapeutic use*; D018139:Simplexvirus / Q000187:drug effects*; D013329:Structure-Activity Relationship; D014414:Tungsten / Q000627:therapeutic use*; D017973:Tungsten Compounds; D014709:Vero Cells; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004279:DNA, Viral; D011108:Polymers; D017973:Tungsten Compounds; C064098:polyoxotungstate PM 19; D014414:Tungsten