[Effects of various growth factors on the growth of trophoblast cells in long-term culture].
Trophoblasts taken from placental tissue of the 1st trimester and molar tissue, and GCH-1 (gestational choriocarcinoma cell line) cells were cultured in collagen coated dishes. The medium used was a mixture of DME and Ham's F-12 (1:1), containing EGF, PDGF, insulin, GM-CSF, IL-1, -2, -3, PGE1 and PGE2 in various concentrations. 3H-TdR uptake of the cultured cells was measured as a marker of cell growth. The growth of normal trophoblasts was enhanced by PDGF or insulin, and remarkably by EGF + PDGF + insulin + GM-CSF. The growth of molar trophoblasts was accelerated by EGF or insulin. Under these culture conditions, normal trophoblasts have been successfully cultured and maintained for 12 months and molar trophoblasts for 9 months to date. The cultured cells were identified with trophoblasts by immunohistochemical staining with CAM 5.2 monoclonal antibody. No effect of growth factors was observed in GCH-1 cells.
Aoki
Y
Y
Department of Obstetrics and Gynecology, Niigata University School of Medicine.
D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011247:Pregnancy; D014328:Trophoblastic Neoplasms / Q000473:pathology; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D006133:Growth Substances; D007328:Insulin; D007375:Interleukin-1; D007376:Interleukin-2; D007377:Interleukin-3; D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000527:Alprostadil; D015232:Dinoprostone