Cyclosporin synthetase is a 1.4 MDa multienzyme polypeptide. Re-evaluation of the molecular mass of various peptide synthetases.
The earlier determined molecular mass of 0.8 MDa for the multifunctional polypeptide, cyclosporin synthetase, was re-evaluated by SDS-PAGE and CsCl density gradient centrifugation. In SDS-PAGE, new molecular mass values as standards were available from sequencing data. In the CsCl density gradient extremely low protein concentrations, such as 10-50 nM could be analysed due to the fluorescence detection system of the analytical ultracentrifuge. Both methods yielded approximately the same value of about 1.4 MDa. Using this molecular mass of cyclosporin synthetase as a reference the molecular masses of various related enzymes could be re-evaluated in SDS-PAGE. The sedimentation coefficient of 26.3 S for cyclosporin synthetase indicates an oblate overall shape of the enzyme.
Schmidt
B
B
Institut für Physikalische Biologie, Heinrich-Heine Universität Düsseldorf, Germany.
Riesner
D
D
Lawen
A
A
Kleinkauf
H
H
D002586:Cesium; D002712:Chlorides; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry*; D010453:Peptide Synthases / Q000737:chemistry*; D014461:Ultracentrifugation
D002712:Chlorides; D005456:Fluorescent Dyes; D009097:Multienzyme Complexes; C048525:cyclosporin A synthetase; D002586:Cesium; D010453:Peptide Synthases; C028019:cesium chloride