Increased inositol phosphate accumulation in platelets from patients with NIDDM.
We evaluated thrombin-induced inositol phosphate accumulation in [3H]inositol-labeled platelets prepared from patients with non-insulin-dependent diabetes mellitus. There were no significant differences in [3H]inositol incorporation into and contents of phosphoinositides between the diabetic patients and their age-matched control subjects. Thrombin induced a dose- and time-dependent accumulation of inositol phosphate. The accumulation of [3H]inositol trisphosphate and [3H]inositol bisphosphate by thrombin stimulation were significantly enhanced in platelets from the diabetic patients, although the accumulation of [3H]inositol monophosphate did not differ between the diabetic patients and the control subjects. In addition, the platelet aggregation rate induced by thrombin was also significantly enhanced in the diabetic patients in correlation with the enhanced inositol phosphate accumulation. These results suggest that increased inositol phosphate accumulation may cause accelerated platelet functions in diabetes mellitus.
Ishii
H
H
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Umeda
F
F
Hashimoto
T
T
Nawata
H
H
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D002784:Cholesterol / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D005337:Fibrin / Q000494:pharmacology; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D007294:Inositol / Q000097:blood; D007295:Inositol Phosphates / Q000097:blood*; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010716:Phosphatidylinositols / Q000097:blood; D010974:Platelet Aggregation / Q000187:drug effects; D012016:Reference Values; D014280:Triglycerides / Q000097:blood; D014316:Tritium
D002784:Cholesterol; D005337:Fibrin; D006442:Glycated Hemoglobin; D007294:Inositol; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D014280:Triglycerides; D014316:Tritium