title: Evaluation of NaOH treatment of human dura mater implants to obviate Creutzfeldt-Jakob disease transmission.
abstract: One of the few disinfectants known to inactivate the causative agent of Creutzfeldt-Jakob Disease is NaOH. In this study, NaOH was evaluated as a possible routine treatment for human dura mater alloimplants. Use of high concentration NaOH (1 M) resulted in protein loss and macroscopic changes to the tissue. Lower concentrations (0.1 M), although exhibiting little direct detrimental effect, greatly increased the susceptibility of the tissue to collagenase digestion. The use of NaOH treated commercial or institutionally prepared human dura mater should be approached with caution.
authors.affiliation: Yorkshire Regional Tissue Bank, Pinderfields General Hospital, Wakefield, UK.
authors.forename: J N
authors.initials: JN
authors.lastname: Kearney
authors.forename: C
authors.initials: C
authors.lastname: Johnson
mesh_terms: D007562:Creutzfeldt-Jakob Syndrome / Q000517:prevention & control*; D004388:Dura Mater / Q000187:drug effects / Q000637:transplantation*; D006801:Humans; D066298:In Vitro Techniques; D003012:Microbial Collagenase / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000494:pharmacology*
chemical_list: D012972:Sodium Hydroxide; D003012:Microbial Collagenase