title: A soluble form of the human T cell differentiation antigen CD27 is released after triggering of the TCR/CD3 complex.
abstract: The human T cell Ag CD27 belongs to a recently defined family of cell surface receptors, including the nerve growth factor receptor, two distinct tumor necrosis factor receptors, and the B cell specific molecule CD40. On resting T cells, CD27 is a transmembrane homodimer with subunits of 50 to 55 kDa (p55). T cell activation via the TCR/CD3 complex causes a strong enhancement of p55 expression. Concomitantly, an alternative form of the CD27 molecule with a molecular mass of 28 to 32 kDa (p32) appears at the cell surface. With the use of ELISA, we here show that a soluble form of CD27 (sCD27) can be detected in the supernatant of T cells activated with anti-CD3 or combinations of anti-CD2 mAb. Moreover, sCD27 is found in both serum and urine from healthy donors. sCD27, purified from either culture supernatant or urine, has a molecular mass of 28 to 32 kDa and is, according to peptide mapping, structurally homologous to the p55 membrane form of CD27. Quantification of sCD27 levels may be used as a marker for T lymphocyte activation in vivo.
authors.affiliation: Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: R Q
authors.initials: RQ
authors.lastname: Hintzen
authors.forename: R
authors.initials: R
authors.lastname: de Jong
authors.forename: C E
authors.initials: CE
authors.lastname: Hack
authors.forename: M
authors.initials: M
authors.lastname: Chamuleau
authors.forename: E F
authors.initials: EF
authors.lastname: de Vries
authors.forename: I J
authors.initials: IJ
authors.lastname: ten Berge
authors.forename: J
authors.initials: J
authors.lastname: Borst
authors.forename: R A
authors.initials: RA
authors.lastname: van Lier
mesh_terms: D015703:Antigens, CD / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D017252:CD3 Complex; D002462:Cell Membrane / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism; D010449:Peptide Mapping; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D015398:Signal Transduction; D012995:Solubility; D013601:T-Lymphocytes / Q000502:physiology*; D013997:Time Factors; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7