Plasmids with easily excisable xylE cassettes.
Two new vectors containing the xylE gene (encoding catechol-2,3-dioxygenase) of Pseudomonas putida were constructed that serve as the source of the xylE cassette. These vectors are based on the kanamycin-resistance-encoding plasmid, pKAN18. The promoter-less xylE gene is flanked by several restriction enzyme sites that allow for easy excision of this gene in the form of a cassette containing a ribosome-binding site, 7 bp upstream from the start codon. These cassettes lack any transcriptional termination signals downstream from the stop codon.
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Department of Microbiology, University of Maryland, College Park 20742.
D001483:Base Sequence; D050557:Catechol 2,3-Dioxygenase; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D049308:Dioxygenases; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase