Molecular cloning and characterization of a rat A1-adenosine receptor that is widely expressed in brain and spinal cord.
An A1-adenosine receptor has been cloned from a rat brain cDNA library using a probe generated by the polymerase chain reaction. The cDNA encodes a protein of 327 amino acids which is 91% identical to a recently cloned dog A1-adenosine receptor (RDC7). Expression of the rat cDNA in COS-6M and NIH 3T3 cells resulted in ligand binding and functional activity characteristics of an A1-adenosine receptor that is coupled to inhibition of adenylyl cyclase. Examination of the distribution of A1-adenosine receptor mRNA by Northern blot analysis showed that it is highly expressed in brain, spinal cord, testis, and white adipose tissue. In situ hybridization studies revealed an extensive hybridization pattern in the central nervous system, with high levels in cerebral cortex, hippocampus, cerebellum, thalamus, brainstem, and spinal cord. The cloned A1-adenosine receptor may thus mediate many of the modulatory actions of adenosine in neural and endocrine systems.
Reppert
S M
SM
Laboratory of Developmental Chronobiology, Children's Service, Massachusetts General Hospital, Boston.
Weaver
D R
DR
Stehle
J H
JH
Rivkees
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001923:Brain Chemistry; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011983:Receptors, Purinergic / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013116:Spinal Cord / Q000737:chemistry*; D014018:Tissue Distribution; D014162:Transfection
D012333:RNA, Messenger; D011983:Receptors, Purinergic; D004247:DNA; D000242:Cyclic AMP