Development of IL-2-independent feline lymphoid cell lines chronically infected with feline immunodeficiency virus: importance for diagnostic reagents and vaccines.
Two interleukin 2 (IL-2)-independent feline immunodeficiency virus (FIV) producer cell lines (FL-4 and FL-6) were produced by selecting cells from an IL-2-dependent culture of mixed peripheral blood lymphocytes infected with FIV. The new cell lines have been stable for over 1 year and spontaneously produce FIV with an average reverse transcriptase titer of 300,000 cpm/ml and an average sucrose gradient purified viral protein concentration of 1 mg/l. FIV produced from these cultures is highly infectious in vitro and in vivo. The FL-6 cell line was phenotyped as expressing the feline CD8 and Pan-T antigens, while the FL-4 cell line expressed the CD4, CD8, and Pan-T antigens. Both cell lines, however, express high levels of viral core and envelope proteins. Paraformaldehyde-inactivated whole virus and similarly inactivated whole-cell virus preparations induced a strong antibody response to core and envelope antigens in immunized cats. The establishment of FIV-producing feline IL-2-independent peripheral blood lymphocyte lines should be valuable for the development of FIV-diagnostic reagents and vaccines and also as a model for human acquired immunodeficiency syndrome vaccine development.
Yamamoto
J K
JK
Department of Medicine, MS-1A, School of Veterinary Medicine, University of California, Davis 95616.
Ackley
C D
CD
Zochlinski
H
H
Louie
H
H
Pembroke
E
E
Torten
M
M
Hansen
H
H
Munn
R
R
Okuda
T
T
D000818:Animals; D002415:Cats; D002460:Cell Line; D016089:Immunodeficiency Virus, Feline / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D007376:Interleukin-2 / Q000494:pharmacology; D007700:Kinetics; D008214:Lymphocytes / Q000382:microbiology*; D014776:Virus Cultivation
D007376:Interleukin-2