Detection and characterization of naturally occurring plasmids in Bacillus licheniformis.
Twenty-two Bacillus licheniformis strains, freshly isolated from pasture-land, were studied for the presence of plasmid DNA. Among these strains, 14 were shown to harbor one or more plasmids of different size. Southern-hybridization experiments showed a high homology between all plasmids investigated and a 2.2-kb PvuII/HindIII fragment of pBL1, a B. licheniformis plasmid previously isolated. Three fragments of pBL1, including the 2.2-kb PvuII/HindIII region, were cloned into pJH101 vector. The resulting chimeras were able to transform Bacillus subtilis. The fragment with high homology probably contains the region with the replicative functions of plasmids from B. licheniformis species.
Parini
C
C
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, Italy.
Fortina
M G
MG
Manachini
P L
PL
De Rossi
E
E
Riccardi
G
G
D001407:Bacillus / Q000187:drug effects / Q000235:genetics*; D015139:Blotting, Southern; D002678:Chimera / Q000235:genetics; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005822:Genetic Vectors / Q000235:genetics; D008670:Metals / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D008670:Metals; D004262:DNA Restriction Enzymes