Effects of dimethylsulfoxide on the oxidative metabolism of certain tissues in rats in vitro.
Administration of 10 mM dimethylsulfoxide inhibits oxygen uptake in in vitro rat brain slices incubated in a Krebs-Ringer phosphate pH 7.4 solution containing 10 mM glucose. The effect is inverted when excessive potassium is added to the solution and the amount of sodium chloride is substituted by choline chloride. Using 10 mM, we observed a decrease in oxygen uptake in rat heart slices incubated in a Krebs-Ringer phosphate solution containing 131.8 mM Na+, 105.4 mM K+ and no calcium. Dimethylsulfoxide 10 mM, 1 mM and 0.1 mM does not alter the Na+/K+ ATPase activity nor the ouabain insensitive ATPase activity in in vitro preparations of rat brain, kidney and heart.
Velasco
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Dept. of Pharmacology, Faculty of Medicine, University of Valladolid, Spain.
Dueñas-Laita
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Molina
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D000251:Adenosine Triphosphatases / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D007552:Isotonic Solutions / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism
D007552:Isotonic Solutions; C051865:Krebs-Ringer solution; D010042:Ouabain; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose; D010100:Oxygen; D004121:Dimethyl Sulfoxide