Abnormalities of chromosome 1 and loss of heterozygosity on 1p in primary hepatomas.
Cytogenetic analysis of eight human hepatoma-derived cell lines and one primary hepatocellular carcinoma biopsy revealed multiple chromosome abnormalities; however, only chromosome 1 was consistently affected by rearrangements. Pseudopolysomy 1 as well as chromosome 1 deletions and/or translocations that resulted in loss of the distal 1p region from at least one copy of chromosome 1 were observed in all but one of the cell lines analysed. Molecular analyses of tumor-derived and normal genomic DNA from six cases of hepatocellular carcinoma and from two of hepatoblastoma, using a panel of chromosome 1p-specific DNA probes indicated allelic loss in the distal 1p region in five of the six hepatocellular carcinomas but not in either hepatoblastoma. These results suggest the location of a gene in the distal 1p region whose functional loss may be involved in hepatocellular carcinogenesis.
Simon
D
D
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Knowles
B B
BB
Weith
A
A
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000382:microbiology; D002460:Cell Line; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002878:Chromosomes, Human, Pair 1; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015321:Gene Rearrangement; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification; D006579:Heterozygote; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000382:microbiology; D014178:Translocation, Genetic
D015342:DNA Probes; D004273:DNA, Neoplasm