title: Functional expression of P2Y purinoceptor in Xenopus oocyte injected with brain mRNA.
abstract: Xenopus oocytes injected with embryonic guinea-pig brain mRNA expressed functional P2Y purinoceptors. Extracellular ATP stimulated in a dose-dependent manner a delayed Ca(2+)-dependent Cl- current component. Analysis of the interactions of ATP with compounds that affect Ca2+ fluxes through the plasma membrane or Ca2+ release from internal stores indicates that ATP raises [Ca2+]i by a mechanism that involves activation of voltage-dependent Ca2+ channels, which leads to influx of extracellular Ca2+ into the cells, as well as release of Ca2+ from intracellular stores. Since this phenomenon was not found in control oocytes, it is suggested that brain mRNA encoded for a newly synthesized Ca(2+)-release process stimulated by purinoceptor activation. This mechanism could be largely involved in the short-term regulation of intracellular Ca2+ level involved in ATP neuromodulation functions.
authors.affiliation: Laboratorie de Physiologie Cellulaire UFR de Biologie, Université des Sciences et Techniques de Lille-Flandres-Artois, Villeneuve d'Ascq, France.
authors.forename: E
authors.initials: E
authors.lastname: Honoré
authors.forename: F
authors.initials: F
authors.lastname: Fournier
authors.forename: T
authors.initials: T
authors.lastname: Collin
authors.forename: J
authors.initials: J
authors.lastname: Nargeot
authors.forename: P
authors.initials: P
authors.lastname: Guilbault
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism / Q000502:physiology; D015220:Calcium Channels / Q000502:physiology; D002712:Chlorides / Q000502:physiology; D004594:Electrophysiology; D006168:Guinea Pigs / Q000196:embryology / Q000378:metabolism; D007267:Injections; D009865:Oocytes / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism / Q000494:pharmacology*; D011983:Receptors, Purinergic / Q000378:metabolism*; D014981:Xenopus
chemical_list: D015220:Calcium Channels; D002712:Chlorides; D012333:RNA, Messenger; D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate; D002118:Calcium