title: Internal initiation and frameshifting in infectious bursal disease virus sequence expressed in Escherichia coli.
abstract: As part of our efforts to produce native molecules of the host protective antigen (VP2) of infectious bursal disease virus (IBDV) in microorganisms, we have restored nucleotide sequences encoding the VP2 N-terminus which were not present in the original clone. In addition to this process, we produced constructs that contained +5, +4, -1, and -2 frameshifts that still allowed expression of the IBDV polyprotein, VP2 and VP3. The size of translation products and examination of the nucleotide sequence lead us to speculate that both +1 frameshifting and internal initiation events lead to the synthesis of IBDV proteins in Escherichia coli.
authors.affiliation: CSIRO, Division of Biomolecular Engineering, Parkville, Australia.
authors.forename: I G
authors.initials: IG
authors.lastname: Macreadie
authors.forename: A A
authors.initials: AA
authors.lastname: Azad
mesh_terms: D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D007243:Infectious bursal disease virus / Q000235:genetics*; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D011506:Proteins; D012367:RNA, Viral; D014764:Viral Proteins