The hygromycin-resistance-encoding gene as a selection marker for vaccinia virus recombinants.
Hygromycin B (Hy), an inhibitor of RNA translation, was shown to block the replication of vaccinia virus (VV) in cultured cell lines. Insertion of the Escherichia coli Hy resistance-encoding gene (hph) into the VV genome under control of early or late synthetic VV promoters could overcome inhibition of viral replication. When hph was inserted into VV in tandem with the human papillomavirus type 16 (HPV16) L1 open reading frame, hph recombinant viruses could be selected which expressed HPV16 L1.
Zhou
J
J
Department of Medicine, Princess Alexandra Hospital, Woolloongabba, Australia.
Crawford
L
L
Sun
X Y
XY
Frazer
I H
IH
D001483:Base Sequence; D002934:Cinnamates; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006921:Hygromycin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D014176:Protein Biosynthesis / Q000187:drug effects; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000187:drug effects / Q000235:genetics*; D014779:Virus Replication / Q000187:drug effects
D002934:Cinnamates; D004274:DNA, Recombinant; D005819:Genetic Markers; D006921:Hygromycin B; C026273:hygromycin A