Cell-free, de novo synthesis of poliovirus.
Cell-free translation of poliovirus RNA in an extract of uninfected human (HeLa) cells yielded viral proteins through proteolysis of the polyprotein. In the extract, newly synthesized proteins catalyzed poliovirus-specific RNA synthesis, and formed infectious poliovirus de novo. Newly formed virions were neutralized by type-specific antiserum, and infection of human cells with them was prevented by poliovirus receptor-specific antibodies. Poliovirus synthesis was increased nearly 70-fold when nucleoside triphosphates were added, but it was abolished in the presence of inhibitors of translation or viral genome replication. The ability to conduct cell-free synthesis of poliovirus will aid in the study of picornavirus proliferation and in the search for the control of picornaviral disease.
Molla
A
A
Department of Microbiology, School of Medicine, State University of New York, Stony Brook 11794.
Paul
A V
AV
Wimmer
E
E
D001483:Base Sequence; D002474:Cell-Free System; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D017955:Poliovirus / Q000254:growth & development*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis / Q000737:chemistry; D014779:Virus Replication
D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D014764:Viral Proteins