Monoclonal antibody KOLT-2 enhances DNA synthesis and expression of IL-2 receptors on activated T cells.
The functional properties of the KOLT-2 antigen, which was placed among the CD28 group in the 3rd International Workshop on Human Leukocyte Differentiation Antigens, are described. The method of production and a detailed characterization of the monoclonal antibody KOLT-2 including stain affinities and influence of T cell activation are presented. While KOLT-2 antigen was selectively expressed on human thymocytes, adult T-cell leukemia-lymphoma cells and activated T cells, the antigen was also spontaneously expressed by resting T cells after 24 hrs culture. Expression was transient, since it disappeared after 96 hrs. DNA synthesis and expression of Tac antigen on activated T cells was enhanced by stimulation with the KOLT-2. These observations suggest that the KOLT-2 antigen may have an important role in the early stages of T cell activation.
Okubo
K
K
Department of Dermatology, Kurume University School of Medicine, Japan.
Hachisuka
H
H
Karashima
T
T
Sasai
Y
Y
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D018106:CD28 Antigens; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018106:CD28 Antigens; D015375:Receptors, Interleukin-2; D004247:DNA