title: Characterization of a novel receptor in toad retina with dual specificity for insulin and insulin-like growth factor I.
abstract: The biochemical properties of insulin receptors from toad retinal membranes were examined in an effort to gain insight into the role this receptor plays in the retina. Competition binding assays revealed that toad retinal membranes contained binding sites that displayed an equal affinity for insulin and insulin-like growth factor I (IGF-I). Affinity labeling of toad retinal membrane proteins with 125I-insulin resulted in the specific labeling of insulin receptor alpha-subunits of approximately 105 kDa. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of partially reduced (alpha beta-heterodimer) receptors affinity-labeled with 125I-insulin indicated the presence of a disulfide-linked beta-subunit of approximately 95 kDa. Endoglycosidase F digestion of the affinity-labeled alpha-subunits increased their mobility by reducing their apparent mass to approximately 83 kDa. This receptor was not detected by immunoblot analysis with a site-specific antipeptide antibody directed against residues 657-670 of the carboxy terminal of the human insulin receptor alpha-subunit, whereas this antibody did label insulin receptor alpha-subunits from pig, cow, rabbit, and chick retinas. In in vitro autophosphorylation assays insulin stimulated the tyrosine phosphorylation of toad retina insulin receptor beta-subunits. These data indicate that toad retinal insulin receptors have a heterotetrameric structure whose alpha-subunits are smaller than other previously reported neuronal insulin receptors. They further suggest that a single receptor may account for both the insulin and IGF-I binding activities associated with toad retinal membranes.
authors.affiliation: Department of Ophthalmology, Yale University School of Medicine, New Haven, Connecticut.
authors.forename: C
authors.initials: C
authors.lastname: Zetterström
authors.forename: C
authors.initials: C
authors.lastname: Fang
authors.forename: A
authors.initials: A
authors.lastname: Benjamin
authors.forename: S A
authors.initials: SA
authors.lastname: Rosenzweig
mesh_terms: D000818:Animals; D001001:Anura / Q000378:metabolism*; D001667:Binding, Competitive; D002417:Cattle; D015151:Immunoblotting; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D011972:Receptor, Insulin / Q000737:chemistry / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017451:Receptors, Somatomedin; D012160:Retina / Q000378:metabolism*; D013002:Somatomedins / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D011972:Receptor, Insulin