A novel P-type ATPase from yeast involved in sodium transport.
The gene ENA1 was cloned by its ability to complement the Li+ sensitivity of a low Li(+)-efflux strain. The nucleotide sequence of the cloned DNA fragment showed that there are two almost identical genes in tandem, and predicts that they encode P-ATPases. Disruption of both genes originated a strain defective in Na+ and Li+ effluxes, and sensitive to Na+, to Li+ and to alkaline pH. By transformation with ENA1 the defective effluxes and tolerances were repaired.
Haro
R
R
Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos, Madrid, Spain.
Garciadeblas
B
B
Rodríguez-Navarro
A
A
D001693:Biological Transport, Active; D006863:Hydrogen-Ion Concentration; D008094:Lithium / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism / Q000502:physiology; D011188:Potassium / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D000254:Sodium-Potassium-Exchanging ATPase / Q000145:classification / Q000378:metabolism* / Q000502:physiology*; D014170:Transformation, Genetic
D010750:Phosphoproteins; D008094:Lithium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium