[Stimulated chemiluminescence and degranulation of human polymorphonuclear leukocytes: possible involvement in the mechanisms of tissue damage during inflammation].
A significant increase of polymorphonuclear leukocytes (PMN) chemiluminescence (CL) was observed when PMN was treated with rat C5ades Arg (r-C5ai), FMLP, opsozined zymosan (STZ) or a calcium ionophore A23487 separately. These stimuli, as well as aggregated IgG (A-IgG), could also cause the release of beta-glucuronidase (beta-g) and myeloperoxidase (MPO) from PMN, on the other hand, elastase (NE) release was not noticed when PMN was treated with r-C5ai and FMLP, which generally stimulated PMN in a cytochalasin B-dependent manner. These results suggest that the kinetics of PMN CL and degranulation vary depending upon the stimulus.
Cui
X
X
Institute of Basic Medical Sciences, Beijing.
D000001:Calcimycin / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects*; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000201:enzymology / Q000502:physiology*; D010196:Pancreatic Elastase / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology
D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D009195:Peroxidase; D005966:Glucuronidase; D010196:Pancreatic Elastase