title: Transposase contacts with mu DNA ends.
abstract: We have investigated the interaction between phage Mu transposase (A protein) and the ends (att sites) of Mu by chemical and nuclease protection and interference studies. These studies define a 24-base pair contact region at five of the six att sites (L1, L3 at att L and R1, R2, R3 at att R). Hydroxyl radical footprints show that the transposase binds to one face of the DNA helix and covers two consecutive major grooves. Binding specificity is achieved primarily through the major groove. Strong contacts are found with 3 guanines which are conserved at five of the sites. Two of these guanines are missing in the weakest binding site (L2) where 13 base pairs are mainly contacted. A pair of DNAase I hypersensitive sites, one on each strand, appear at the back of only one of the two contacted major grooves at most sites except at L2, and can be correlated with the degree of A protein-induced bend (Kuo, C.-F., Zou, A., Jayaram, M., Getzoff, E. D., and Harshey, R. M. (1991) EMBO J. 10, 1585-1591) at these sites. No contacts are observed for 4-5 base pairs in the vicinity of L1 and R1, where the A protein nicks DNA during transposition.
authors.affiliation: Department of Microbiology, University of Texas, Austin 78712.
authors.forename: A H
authors.initials: AH
authors.lastname: Zou
authors.forename: P C
authors.initials: PC
authors.lastname: Leung
authors.forename: R M
authors.initials: RM
authors.lastname: Harshey
mesh_terms: D010583:Bacteriophage mu / Q000201:enzymology*; D001483:Base Sequence; D016172:DNA Fingerprinting; D004591:Electrophoresis, Polyacrylamide Gel; D006878:Hydroxides; D017665:Hydroxyl Radical; D008745:Methylation; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D019895:Transposases
chemical_list: D006878:Hydroxides; D017665:Hydroxyl Radical; D009713:Nucleotidyltransferases; D019895:Transposases