title: EGF-R as a hemopoietic growth factor receptor: the c-erbB product is present in chicken erythrocytic progenitors and controls their self-renewal.
abstract: c-erbB, encoding the EGF receptor (EGF-R), was originally identified as the cellular homolog of a chicken leukemia oncogene. In humans, EGF-R is distributed widely except in hemopoietic tissues, and its amplification is associated with epidermal and glial malignancies. Here we show that c-erbB is present in normal chicken erythrocytic progenitors and transmits the mitogenic signal induced by TGF alpha. Cells that contain high affinity EGF-R are at approximately the BFU-E stage, and their long-term renewal can be induced by TGF alpha. Upon addition of insulin and erythropoietin, they can be induced to terminally differentiate into red cells. We previously demonstrated that v-erbA blocks differentiation of chicken erythrocytic progenitors but does not abrogate their growth factor dependence for proliferation. These data indicate that proliferation and differentiation are not necessarily coupled in these cells. They also demonstrate a direct role of c-erbB in the control of self-renewal of normal chicken erythrocytic progenitors and could account for the predominant leukemogenic potential of the chicken erbB gene.
authors.affiliation: Laboratoire de Biologie Moléculaire et Cellulaire, CNRS INRA, Ecole Normale Supérieure de Lyon, France.
authors.forename: B
authors.initials: B
authors.lastname: Pain
authors.forename: C M
authors.initials: CM
authors.lastname: Woods
authors.forename: J
authors.initials: J
authors.lastname: Saez
authors.forename: T
authors.initials: T
authors.lastname: Flickinger
authors.forename: M
authors.initials: M
authors.lastname: Raines
authors.forename: S
authors.initials: S
authors.lastname: Peyrol
authors.forename: C
authors.initials: C
authors.lastname: Moscovici
authors.forename: M G
authors.initials: MG
authors.lastname: Moscovici
authors.forename: H J
authors.initials: HJ
authors.lastname: Kung
authors.forename: P
authors.initials: P
authors.lastname: Jurdic
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002455:Cell Division; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002645:Chickens; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004921:Erythropoietin / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D018774:Oncogene Proteins v-erbA; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D016184:Receptors, Colony-Stimulating Factor / Q000378:metabolism*; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics; D016211:Transforming Growth Factor alpha / Q000494:pharmacology
chemical_list: D007328:Insulin; D018774:Oncogene Proteins v-erbA; D011518:Proto-Oncogene Proteins; D016184:Receptors, Colony-Stimulating Factor; D015682:Retroviridae Proteins, Oncogenic; D016211:Transforming Growth Factor alpha; D004921:Erythropoietin; D066246:ErbB Receptors