High expression of human cytomegalovirus (HCMV)-gB protein in cells infected with a vaccinia-gB recombinant: the importance of the gB protein in HCMV immunity.
The human cytomegalovirus (HCMV), Towne strain, glycoprotein B (gB) gene was cloned into a vaccinia vector (Copenhagen strain) under the control of the H6 early and late vaccinia promoters (Vac-gB recombinant). The gB protein was expressed in a high percentage of the Vac-gB-infected cells throughout the virus replication cycle. Cytosine-arabinoside (ara-C) did not influence the expression of the gB protein early after infection (5 h), but did inhibit it later in viral replication (7-29 h). The Vac-gB recombinant induced HCMV neutralizing antibodies in guinea-pigs. Cells infected with the Vac-gB recombinant absorbed 50-88% of neutralizing activity of human sera obtained from volunteers previously inoculated with the Towne or Toledo strains and from naturally seropositive individuals.
Gönczöl
E
E
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
deTaisne
C
C
Hirka
G
G
Berencsi
K
K
Lin
W C
WC
Paoletti
E
E
Plotkin
S
S
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D006224:Cricetinae; D003561:Cytarabine / Q000494:pharmacology; D003587:Cytomegalovirus / Q000276:immunology*; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D011995:Recombination, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D003561:Cytarabine