Immunobiological activities of synthetic peptide segments of fimbrial protein from Porphyromonas gingivalis.
Several oligopeptide segments of fimbrial subunit protein (fimbrilin) of Porphyromonas gingivalis strain 381 were synthesized and tested for immunobiological activities. Peptides F3(31-50; amino acid residue numbers 31 to 50, based on the amino acid sequence of the fimbrilin proposed by Dickinson et al., Infect. Immun., 170, 1658, 1988), F12(212-231) and F17(312-331) were found to be immunodominant epitopes of this fimbrial protein as revealed by ELISA. Furthermore, peptides F5(71-90) and F17(312-331) were demonstrated to agglutinate rabbit erythrocytes, and were mitogenic for BALB/c spleen cells but not thymocytes. These peptides enhanced the number of fimbria-specific antibody-secreting cells in BALB/c spleen cell cultures, and induced cytokines such as tumor necrosis factor-alpha and interleukin-6 production in human monocyte/macrophage cultures. The data demonstrate that these defined peptide segments are responsible for the immunostimulating portions within the fimbrial protein molecule.
Ogawa
T
T
Department of Oral Microbiology, Osaka University, Faculty of Dentistry, Japan.
Kusumoto
Y
Y
Uchida
H
H
Nagashima
S
S
Ogo
H
H
Hamada
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology; D036781:Fimbriae Proteins; D006384:Hemagglutination; D006801:Humans; D007739:L Cells; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology* / Q000494:pharmacology; D016966:Porphyromonas gingivalis / Q000276:immunology*; D011817:Rabbits; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D001426:Bacterial Proteins; D010446:Peptide Fragments; D014409:Tumor Necrosis Factor-alpha; C063055:fimbrillin; D036781:Fimbriae Proteins