title: cDNA clones of Japanese hepatitis C virus genomes derived from a single patient show sequence heterogeneity.
abstract: Twelve cDNA clones of Japanese hepatitis C virus (HCV) have been isolated from liver tissue of a single non-A, non-B hepatitis patient. These clones represented the non-structural domains of HCV. The degree of substitution in the nucleotide sequences and deduced amino acid sequences between these clones was 9.5 and 7.7%, respectively. This high level of substitution suggested that repeated infections of different HCVs may have occurred in the patient.
authors.affiliation: Fuji-Gotemba Research Laboratories, Chugai Pharmaceutical Co. Ltd, Shizuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Oshima
authors.forename: M
authors.initials: M
authors.lastname: Tsuchiya
authors.forename: M
authors.initials: M
authors.lastname: Yagasaki
authors.forename: T
authors.initials: T
authors.lastname: Orita
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
authors.forename: K
authors.initials: K
authors.lastname: Tomonoh
authors.forename: T
authors.initials: T
authors.lastname: Kojima
authors.forename: Y
authors.initials: Y
authors.lastname: Hirata
authors.forename: O
authors.initials: O
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Sho
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral; D005814:Genes, Viral; D014644:Genetic Variation; D015698:Genomic Library; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000382:microbiology*; D006801:Humans; D007564:Japan; D008099:Liver / Q000382:microbiology; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral