Amyloid beta protein substituent peptides do not interact with the substance P receptor expressed in cultured cells.
The amyloid beta protein (ABP) has been shown to interact with the substance P (SP) receptor in a cell culture model that may mimic the pathogenesis of Alzheimer's disease. In the present study, however, 4 fragments of ABP (beta 1-42, beta 1-16, beta 17-28, and beta 25-35) failed to interact with SP-induced Ca2+ mobilization in SP receptor-expressing cultured cells. Therefore, the action of these ABP-related peptides in our cultured cells is unrelated to the SP receptor.
Mitsuhashi
M
M
Division of Medical Sciences, Hitachi Chemical Research Center, Inc., Irvine, CA 92715.
Akitaya
T
T
Turk
C W
CW
Payan
D G
DG
D000544:Alzheimer Disease / Q000209:etiology / Q000378:metabolism; D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism*; D051381:Rats; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D009479:Neuropeptides; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D002118:Calcium