title: Enzyme-linked immunosorbent assay methods for detection of feline leukemia virus and feline immunodeficiency virus.
abstract: Enzyme-linked immunosorbent assays have been widely used for diagnosis of FeLV and feline immunodeficiency virus (FIV) infections. Various ELISA kits for FeLV are available from several manufacturers. Although these tests are configured in a variety of formats, they are all direct antigen-detection systems for the viral core protein p27. On the other hand, ELISA for FIV exposure detects specific feline antibody to FIV. Basic immunoassay principles and the application of ELISA technology used in FeLV and FIV ELISA kits are described.
authors.affiliation: IDEXX Corporation, Westbrook, ME 04092.
authors.forename: Q J
authors.initials: QJ
authors.lastname: Tonelli
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay; D016089:Immunodeficiency Virus, Feline / Q000276:immunology / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000175:diagnosis / Q000662:veterinary*; D005256:Leukemia Virus, Feline / Q000276:immunology / Q000302:isolation & purification*; D016582:Leukemia, Feline / Q000175:diagnosis*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic