DCCD binds to cytochrome b6 of a cytochrome bf complex isolated from spinach chloroplasts and inhibits proton translocation.
Radiolabeled N,N'-dicyclohexylcarbodiimide (DCCD) was bound selectively in a time- and concentration-dependent manner to cytochrome b6 of an enzymatically active cytochrome bf complex isolated from spinach chloroplasts. Maximum labeling of cytochrome b6 was observed with 30 nmol DCCD per nmol cytochrome b6 in the cytochrome bf complex incubated for 30-60 min at 12 degrees C. After incubation of the cytochrome bf complex with DCCD under these conditions, the rate of proton ejection in the complex reconstituted into liposomes was decreased approximately 65-70% when compared to controls; however, under these same conditions the rate of electron transfer through either the soluble bf complex or the complex reconstituted into liposomes was only decreased around 20%. These results suggest that the mechanism of proton translocation through the cytochrome bf complex of spinach chloroplasts is similar to that of the cytochrome bc1 complex from yeast mitochondria in which proton pumping but not electron transfer is also inhibited by DCCD (D. S. Beattie and A. Villalobo, 1982, J. Biol. Chem. 257, 14,745-14,752).
Wang
Y D
YD
Department of Biochemistry, West Virginia University School of Medicine, Morgantown 26506.
Beattie
D S
DS
D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000201:enzymology*; D003573:Cytochrome b Group / Q000037:antagonists & inhibitors*; D045346:Cytochrome b6f Complex; D003580:Cytochromes / Q000302:isolation & purification*; D045348:Cytochromes f; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology*; D008969:Molecular Sequence Data; D011522:Protons; D012689:Sequence Homology, Nucleic Acid; D014675:Vegetables
D003573:Cytochrome b Group; D003580:Cytochromes; D011522:Protons; D004024:Dicyclohexylcarbodiimide; D045346:Cytochrome b6f Complex; D045348:Cytochromes f