title: Transcription factor AP-1 modulates the activity of the human foamy virus long terminal repeat.
abstract: The human foamy virus (HFV) contains within the U3 region of its long terminal repeat (LTR) three perfect consensus sequences for the binding of the inducible transcription factor AP-1. Results of DNase I footprint protection and gel retardation assays demonstrated that proteins in extracts of HeLa and BHK-21 cells as well as bacterially expressed Jun and Fos proteins bind to these AP-1 sites. By conducting transient expression assays using chloramphenicol acetyltransferase plasmids carrying LTR sequences with point-mutated AP-1 sites, it was found that the three AP-1 sites contribute to the optimal activity of the HFV promoter. It is shown that induction of the HFV LTR by 12-O-tetradecanoylphorbol-13-acetate (TPA) and serum factors is mediated through the AP-1 sites.
authors.affiliation: Institut für Virologie und Immunbiologie, Universität Würzburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Maurer
authors.forename: E
authors.initials: E
authors.lastname: Serfling
authors.forename: V
authors.initials: V
authors.lastname: ter Meulen
authors.forename: A
authors.initials: A
authors.lastname: Rethwilm
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid / Q000187:drug effects; D015183:Restriction Mapping; D016092:Spumavirus / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection
chemical_list: D004279:DNA, Viral; D016755:Proto-Oncogene Proteins c-jun; D011994:Recombinant Proteins; D013755:Tetradecanoylphorbol Acetate