Protective effect of a new anti-oxidant on the rat brain exposed to ischemia-reperfusion injury: inhibition of free radical formation and lipid peroxidation.
A new oligomeric derivative was synthesized from prostaglandin B2 and ascorbic acid, and its effect on rat brain ischemia-reperfusion injury was studied. Brain ischemia was produced in the rat by the combination of bilateral common carotid artery occlusion and hemorrhagic hypotension (30 mmHg, 20 min). The cerebral cortex was homogenized in the presence of the spin trap agent, N-tert-butyl-alpha-phenyl-nitrone (PBN). Spin-adducts were detected using an electron spin resonance spectrometer (EPR). Lipid peroxidation was estimated from the amounts of both thiobarbituric acid reactive substances (TBAR) and conjugated diene. In control experiments, reperfusion induced a burst of free radical formation which peaked at 5 min reperfusion time (238 +/- 41%). Lipid peroxidation increased significantly after 20 min of reperfusion (TBAR, 161 +/- 50%; conjugated diene, 160 +/- 29%). When the oligomeric derivative was administered (9 mg/kg i.p. 30 min before ischemic insult), it significantly reduced both spin adduct formation (103 +/- 13%) and lipid peroxidation (TBAR, 109 +/- 14%; conjugated diene, 97 +/- 33%).
Sakamoto
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A
Philadelphia Biomedical Research Institute, King of Prussia, PA 19406.
Ohnishi
S T
ST
Ohnishi
T
T
Ogawa
R
R
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004569:Electroencephalography; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006339:Heart Rate / Q000187:drug effects; D002546:Ischemic Attack, Transient / Q000503:physiopathology* / Q000517:prevention & control; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D011456:Prostaglandins B / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000503:physiopathology* / Q000517:prevention & control
D000975:Antioxidants; D005609:Free Radicals; D011456:Prostaglandins B; C070097:OC 5186