Mycoplasma pulmonis infection with regard to embryo freezing and hysterectomy derivation.
Eggs taken from the oviducts of mice, inoculated 3 weeks previously with Mycoplasma pulmonis in the peritoneal cavity, were shown to be contaminated, and mycoplasmas were not eradicated by washing the eggs three times. Zona-free eggs were also infected. Embryos cultured for 24 hours in medium M16 were clear of infection. Sperm from infected males was not significantly worse in the fertilization of uninfected eggs.
Hill
A C
AC
Toxicology Unit, Medical Research Council Laboratories, Carshalton, Surrey, England.
Stalley
G P
GP
D000818:Animals; D001422:Bacterial Adhesion; D015925:Cryopreservation; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000382:microbiology*; D005260:Female; D005307:Fertilization in Vitro; D007044:Hysterectomy; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009175:Mycoplasma Infections / Q000382:microbiology* / Q000517:prevention & control / Q000635:transmission; D010063:Ovum / Q000382:microbiology; D013047:Specific Pathogen-Free Organisms; D013094:Spermatozoa / Q000382:microbiology; D015044:Zona Pellucida / Q000382:microbiology