Analysis of unknown DNA sequences by polymerase chain reaction (PCR) using a single specific primer and a standardized adaptor.
A new procedure for the PCR amplification of unknown DNA sequences adjacent to a known sequence is described. The required but not readily available second primer sequence in the unknown DNA sequence is obtained by creating an overhanging restriction site in the unknown sequence to which a double-stranded oligonucleotide adaptor of known sequence is ligated.
Collasius
M
M
Arbeitsgruppe für Zellbiochemie, Max-Planck-Institut für Biochemie, Martinsried, F.R.G.
Puchta
H
H
Schlenker
S
S
Valet
G
G
D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000737:chemistry*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
D009838:Oligodeoxyribonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes