[Examining the activity of dihydrofolate reduction obtained from beef liver].
The average values of Vm and Michelis constant Km were determined for enzyme isolated from liver (12.23 mumole/min and 3.2 x 10(-6) mole/dm3. respectively). The enzyme was stable over four months at -20 degrees C. Dissociation constant enzyme-inhibitor (methotrexate) complex Ki is 3.7 x 10(-8) mole/dm3. The reaction rate was sensitive to temperature (5 degrees C increase doubled maximum reaction rate).
Skibińska
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Katedra i Zakład Chemii Fizycznej, Akademia Medyczna, Poznań.
D000818:Animals; D002417:Cattle; D008099:Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D013696:Temperature; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D013762:Tetrahydrofolate Dehydrogenase
Badanie aktywności reduktazy dihydrofolianowej otrzymanej z watroby wołowej.