Comparison of characteristics of human spermatozoa selected by the multiple-tube swim-up and simple discontinuous Percoll gradient centrifugation.
The multiple-tube swim-up (MT-SU) and simple, discontinuous Percoll gradient centrifugation are two recent methods for the processing of human spermatozoa for assisted reproductive technologies. The present study was undertaken to compare the effects of these two methods on various parameters of spermatozoal qualities of normospermic semen samples. Both methods were found to select spermatozoa of better percentage motility, percentage normal morphology, viability, functional integrity of plasma membrane, movement characteristics including the curvilinear velocity, linearity of movement and head beat frequency, and in vitro fertilizing capacity with comparable degree of efficacy. It is concluded that both methods are effective for the separation of spermatozoa from normospermic semen samples for use in infertility treatment.
Chan
S Y
SY
Department of Obstetrics and Gynecology, University of Hong Kong.
Chan
Y M
YM
Tucker
M J
MJ
D000177:Acrosome / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D005069:Evaluation Studies as Topic; D005307:Fertilization in Vitro; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D011205:Povidone; D012822:Silicon Dioxide; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone