Metabolic control may influence the increased superoxide generation in diabetic serum.
Superoxide anion (O2-) generation in serum from 10 Type 1 diabetic patients and 10 normal subjects was measured ex vivo. The amount of O2- production was significantly increased in diabetic serum 0.41 +/- 0.04 (+/- SD) vs 0.14 +/- 0.04 mumol l-1 min-1, p less than 0.001) and correlated with fasting plasma glucose and glycosylated protein levels in both diabetic (r = 0.72, p less than 0.01, and r = 0.62, p less than 0.05) and normal r = 0.75, p less than 0.01 and r = 0.64, p less than 0.05) subjects. Improved metabolic control in the diabetic patients was associated with a reduction of serum O2- production (0.28 +/- 0.06 mumol l-1 min-1, p less than 0.01), but the correlation between O2- levels and fasting plasma glucose and glycosylated protein concentrations was retained (r = 0.86 and r = 0.72, respectively, both p less than 0.01).
Ceriello
A
A
Cattedra di Diabetologia e Dietoterapia, I Facoltà di Medicina, Università di Napoli, Italy.
Giugliano
D
D
Quatraro
A
A
Dello Russo
P
P
Lefèbvre
P J
PJ
D000293:Adolescent; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005260:Female; D005609:Free Radicals; D006023:Glycoproteins; D006031:Glycosylation; D006801:Humans; D008297:Male; D013481:Superoxides / Q000097:blood*; D000093422:Glycated Serum Proteins
D001786:Blood Glucose; D001798:Blood Proteins; D005609:Free Radicals; D006023:Glycoproteins; D013481:Superoxides; D000093422:Glycated Serum Proteins