title: Infection of polarized MDCK cells with herpes simplex virus 1: two asymmetrically distributed cell receptors interact with different viral proteins.
abstract: Herpes simplex virus 1 attaches to at least two cell surface receptors. In polarized epithelial (Madin-Darby canine kidney; MDCK) cells one receptor is located in the apical surface and attachment to the cells requires the presence of glycoprotein C in the virus. The second receptor is located in the basal surface and does not require the presence of glycoprotein C. Exposure of MDCK cells at either the apical or basal surface to wild-type virus yields plaques and viral products whereas infection by a glycoprotein C-negative mutant yields identical results only after exposure of MDCK cells to virus at the basal surface. Multiple receptors for viral entry into cells expand the host range of the virus. The observation that glycoprotein C-negative mutants are infectious in many nonpolarized cell lines suggests that cells in culture may express more than one receptor and explains why genes that specify the viral proteins that recognize redundant receptors, like glycoprotein C, are expendable.
authors.affiliation: Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, Chicago, IL 60637.
authors.forename: A E
authors.initials: AE
authors.lastname: Sears
authors.forename: B S
authors.initials: BS
authors.lastname: McGwire
authors.forename: B
authors.initials: B
authors.lastname: Roizman
mesh_terms: D000818:Animals; D002460:Cell Line / Q000382:microbiology*; D002462:Cell Membrane / Q000378:metabolism; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney / Q000166:cytology / Q000382:microbiology / Q000648:ultrastructure; D008562:Membrane Glycoproteins / Q000378:metabolism; D008854:Microscopy, Electron; D018139:Simplexvirus / Q000302:isolation & purification*; D014709:Vero Cells / Q000648:ultrastructure; D014759:Viral Envelope Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C038535:glycoprotein gC, herpes simplex virus type 1