Identification of a dopamine- and 3'5'-cyclic adenosine monophosphate-regulated phosphoprotein of 32 kD (DARPP-32) in parathyroid hormone-producing cells of the human parathyroid gland.
The cellular localization of DARPP-32, a dopamine- and cyclic AMP-regulated phosphoprotein of 32 kD, enriched in dopamine-innervated brain regions, was investigated in the human parathyroid gland using indirect immunofluorescence histochemistry. Monoclonal antibodies were used to demonstrate DARPP-32-like immunoreactivity (LI) in chief cells of the normal human parathyroid gland and in cells of human parathyroid adenoma. Direct double-labelling revealed coexistence of DARPP-32-LI with parathyroid hormone (PTH)-LI. It has previously been demonstrated that dopamine D1-receptors are present in the parathyroid gland and that dopamine and D1-agonists stimulate the release of PTH. The present results suggest that DARPP-32 may play a role in the cellular mechanisms leading to dopamine-induced PTH secretion.
Meister
B
B
Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden.
Askergren
J
J
Tunevall
G
G
Hemmings
H C
HC
Greengard
P
P
D000236:Adenoma / Q000737:chemistry; D000242:Cyclic AMP / Q000502:physiology*; D004298:Dopamine / Q000502:physiology*; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D005455:Fluorescent Antibody Technique; D006801:Humans; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D010280:Parathyroid Glands / Q000166:cytology / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000096:biosynthesis*; D010282:Parathyroid Neoplasms / Q000737:chemistry; D010750:Phosphoproteins / Q000302:isolation & purification*
D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D010281:Parathyroid Hormone; D010750:Phosphoproteins; D000242:Cyclic AMP; D004298:Dopamine