title: Localization of the cellular retinoic acid binding protein (CRABP) gene relative to the acute promyelocytic leukemia-associated breakpoint on human chromosome 15.
abstract: A human genomic fragment comprising the cellular retinoic acid binding protein (CRABP) gene was isolated. By using a panel of somatic cell hybrids, this gene could be assigned to human chromosome 15. Subsequently, a possible involvement of the CRABP gene in translocation (15;17) (q22;q11) positive acute promyelocytic leukemia (APL) was investigated. Although transposition of the CRABP gene could be demonstrated, we did not observe any gross CRABP rearrangement in a series of primary APL patients, nor in the acute myeloblastic leukemia cell line HL-60. Thus, the observed lack of CRABP expression in these leukemic cells may not be caused by disruption of its gene. CRABP maps to the region 15q22-qter.
authors.affiliation: Department of Human Genetics, University of Nijmegen, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Geurts van Kessel
authors.forename: H
authors.initials: H
authors.lastname: de Leeuw
authors.forename: E J
authors.initials: EJ
authors.lastname: Dekker
authors.forename: L
authors.initials: L
authors.lastname: Rijks
authors.forename: N
authors.initials: N
authors.lastname: Spurr
authors.forename: D
authors.initials: D
authors.lastname: Ledbetter
authors.forename: E
authors.initials: E
authors.lastname: Kootwijk
authors.forename: M J
authors.initials: MJ
authors.lastname: Vaessen
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D006801:Humans; D006822:Hybrid Cells; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D018168:Receptors, Retinoic Acid; D014178:Translocation, Genetic
chemical_list: D002352:Carrier Proteins; D009363:Neoplasm Proteins; D018168:Receptors, Retinoic Acid