Use of western blot and radioimmunoprecipitation for diagnosis of feline leukemia and feline immunodeficiency virus infections.
The uses and limitations of the western blot (WB) and radioimmunoprecipitation assay (RIPA) techniques for study of feline immunodeficiency virus (FIV) and FeLV were evaluated. Western blot analysis was used to detect antigenic relatedness between the 2 lentiviruses. Using a rabbit serum directed against p26 of the equine infectious anemia virus (EIAV) and anti-EIAV horse serum obtained from an infected horse, cross-reactivity with p24 of FIV was revealed. Cat sera obtained late after experimentally induced FIV infection recognized p26 of EIAV, which indicates reciprocal cross-reactivity. For RIPA, FIV was metabolically labeled, and virus-coded proteins were identified, using immunoprecipitation. Polypeptides with apparent molecular mass of about 15, 24, 43, 50, 120, and 160 kilodaltons were detected. An additional polypeptide of 10 kilodaltons was found only by use of WB analysis.
Egberink
H F
HF
Department of Infectious Diseases and Immunology, Veterinary Faculty, Institute of Virology, Utrecht, The Netherlands.
Lutz
H
H
Horzinek
M C
MC
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D015153:Blotting, Western; D002415:Cats; D003429:Cross Reactions; D016181:Feline Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D007106:Immune Sera / Q000276:immunology; D016089:Immunodeficiency Virus, Feline / Q000276:immunology / Q000302:isolation & purification*; D005256:Leukemia Virus, Feline / Q000276:immunology / Q000302:isolation & purification*; D016582:Leukemia, Feline / Q000175:diagnosis*; D011237:Predictive Value of Tests; D015531:Radioimmunoprecipitation Assay; D013047:Specific Pathogen-Free Organisms
D000956:Antigens, Viral; D007106:Immune Sera