Biological and physicochemical characterization of the major (1.40) and minor (1.45) component of infectious avian adeno-associated virus.
Two infectious components with buoyant densities of 1.40 g/cm3 and 1.45 g/cm3, designated as major (1.40) and minor (1.45) component, were detected by banding avian adeno-associated virus (AAAV) isopycnically in CsCl. In metrizamide, however, infectious AAAV banded only as a single peak at a density of 1.32 g/cm3. Biological as well as physicochemical properties of the two AAAV components recovered from CsCl density gradient were described. Concerning the minor (1.45) component, three experimental findings may suggest that the capsid structure of this AAAV population is altered in comparison with that of the major (1.40) component: (i) the sedimentation pattern characterized by an additional peak containing slower-sedimenting noninfectious material (16 S); (ii) the specific infectivity decreased by the 3.5 fold; (iii) the ready disintegration when exposed to gently denaturing conditions.
Bauer
H J
HJ
Institut für Geflügelkrankheiten, Freie Universität Berlin, Federal Republic of Germany.
Schneider
R
R
Gelderblom
H R
HR
Lurz
R
R
Friehmelt
V
V
Monreal
G
G
D000818:Animals; D002213:Capsid; D002500:Centrifugation, Isopycnic; D002586:Cesium; D002642:Chick Embryo; D002712:Chlorides; D004279:DNA, Viral / Q000737:chemistry; D000229:Dependovirus / Q000737:chemistry / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008793:Metrizamide
D002712:Chlorides; D004279:DNA, Viral; D002586:Cesium; C028019:cesium chloride; D008793:Metrizamide