Local immune response and bronchial reactivity in rats after capsaicin treatment.
The interaction between the nervous system, immune system and bronchial reactivity was studied in rats by using the neurotoxin capsaicin. Rats were treated with capsaicin at 1-2 days of age or at adult age, before or after sensitization by subcutaneous injections with ovalbumin (OA). The levels of the neuropeptides neurokinin A and calcitonin gene-related peptide were decreased in the lung after capsaicin treatment, as determined with radioimmunoassay, whereas the levels of neuropeptide Y were unaffected. The levels of IgA, IgE and IgG in bronchial lavage were also affected by capsaicin treatment; however, the results were heterogeneous. Capsaicin treatment after sensitization reduced the bronchial reactivity to challenge with OA aerosol and serotonin iv. The results demonstrated that reduction of neuropeptide levels with capsaicin affected both bronchial reactivity and the levels of antibodies in bronchial lavage fluid. However, no correlation between these two parameters was seen, demonstrating the complexity of the system.
Nilsson
G
G
Dept. of Allergy and Immunology, Pharmacia Diagnostics AB, Uppsala, Sweden.
Alving
K
K
Lundberg
J M
JM
Ahlstedt
S
S
D000818:Animals; D001249:Asthma / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism; D001980:Bronchi / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002211:Capsaicin / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D007279:Injections, Subcutaneous; D008168:Lung / Q000737:chemistry / Q000276:immunology; D008297:Male; D015288:Neurokinin A / Q000032:analysis; D009478:Neuropeptide Y / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D011914:Rats, Inbred BN
D009478:Neuropeptide Y; D015288:Neurokinin A; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin