Partial restriction map of salmonid herpesvirus (Oncorhynchus masou virus) DNA for three restriction enzymes BamHI, EcoRI and XhoI.
Cleavage of Oncorhynchus masou virus (OMV) DNA with restriction endonucleases BamHI, EcoRI, and XhoI resulted in 28, 26 and 17 fragments, respectively. Based on the molecular weights of digested fragments and those molar ratio. OMV DNA showed the molecular weight of about 100 x 10(6). Twenty out of 28 BamHI fragments of OMV DNA were successfully cloned into pBR322 vector. Restriction map of OMV DNA was constructed by blotting hybridization and double-digestion. The data suggested that terminal repeat of the end fragments of OMV DNA molecule was not existence.
Guo
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Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Kodama
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Onuma
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D000818:Animals; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D005393:Fish Diseases / Q000382:microbiology; D006564:Herpesviridae / Q000235:genetics*; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D012474:Salmon
D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific