[The evaluation of a commercial EIA test system for determining the specific activity of antiherpetic preparations].
The data on the use of a commercial EIA test system for detection of antibodies in control of preparations against herpes simplex and cytomegaloviruses are presented. The enzyme immunoassay test system for antibody determinations to herpes simplex virus produced by the Odessa bacterial preparations enterprise was shown to be suitable for determination of the specific potency (antigenicity) of herpes simplex vaccine. The advantages of this method over the currently used neutralization test were established. Titration of commercial immunoglobulins detects lots with high litres of antibody to herpes simplex virus. For the same purpose, lots of commercial immunoglobulins were tested for antibodies to cytomegalovirus using a West Germany test-system (Behring). It is concluded that enzyme immunoassay test systems for antibody determinations may be used for screening of lots of immunoglobulins of special effects (against herpes simplex and cytomegalovirus infections) both at the stage of serum and final preparation screening.
Borsuk
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Vorob'eva
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Ebralidze
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D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity / Q000276:immunology*; D003587:Cytomegalovirus / Q000276:immunology; D005069:Evaluation Studies as Topic; D006801:Humans; D007114:Immunization; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D007136:Immunoglobulins / Q000032:analysis / Q000276:immunology*; D009500:Neutralization Tests; D051381:Rats; D011933:Reagent Kits, Diagnostic; D018139:Simplexvirus / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000032:analysis / Q000276:immunology; D014765:Viral Vaccines / Q000032:analysis / Q000276:immunology*
D000914:Antibodies, Viral; D007136:Immunoglobulins; D011933:Reagent Kits, Diagnostic; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
Otsenka kommercheskoĭ test-sistemy IFA dlia opredeleniia spetsificheskoĭ aktivnosti protivogerpeticheskikh preparatov.