Isolation and preliminary characterization of the Porphyromonas gingivalis prtC gene expressing collagenase activity.
A gene, prtC, has been isolated from Porphyromonas gingivalis ATCC 53977 in Escherichia coli utilizing the plasmid vector pPL-lambda. The resultant protease positive clone NHS1, harboring plasmid pS1 with a 5.9-kilobase P. gingivalis insert, expressed an enzyme capable of hydrolyzing the synthetic collagenase substrate PZ-PLGPA as well as solubilized type I collagen. Subcloning and deletion analysis located the prtC gene at one end of the P. gingivalis DNA insert on plasmid pS1.
Takahashi
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Department of Pediatric Density, University of Texas Health Science Center, San Antonio 78284.
Kato
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T
Kuramitsu
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HK
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D003012:Microbial Collagenase / Q000235:genetics* / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping
D003012:Microbial Collagenase