Immunoquantitation of cytochrome C in cardiac perfusate.
An indirect three step ELISA has been assessed in order to detect the possible release of cytochrome c, a mitochondrial protein, from isolated and perfused guinea-pig heart. The ELISA described in this study is sufficiently sensitive and accurate to measure extracellular cytochrome c.
Naro
F
F
Dept. of Biochemical Sciences Univ. of Rome La Sapienza.
Fazzini
A
A
Citro
G
G
Malatesta
F
F
Antonini
G
G
Sarti
P
P
Brunori
M
M
Franconi
F
F
Giotti
A
A
D000818:Animals; D003574:Cytochrome c Group / Q000032:analysis* / Q000378:metabolism / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D010477:Perfusion; D012015:Reference Standards; D012680:Sensitivity and Specificity
D003574:Cytochrome c Group