title: Affinity of integrin alpha 1 beta 1 from liver sinusoidal membranes for type IV collagen.
abstract: Hepatic sinusoidal membranes isolated from adult rats were extracted with detergent and fractionated on a wheat germ agglutinin affinity column. Bound glycoproteins were eluted with N-acetyl glucosamine and chromatographed on a type IV collagen affinity column. Recovery of the bound fraction by EDTA and analysis by SDS-PAGE revealed two glycoproteins with apparent molecular weights of 180,000 and 117,000. These were identified immunologically by Western blotting as the alpha and beta subunits of integrin alpha 1 beta 1.
authors.affiliation: National Institute for Medical Research, London, UK.
authors.forename: S C
authors.initials: SC
authors.lastname: Stamatoglou
authors.forename: S
authors.initials: S
authors.lastname: Bawumia
authors.forename: S
authors.initials: S
authors.lastname: Johansson
authors.forename: E
authors.initials: E
authors.lastname: Forsberg
authors.forename: R C
authors.initials: RC
authors.lastname: Hughes
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity; D003094:Collagen / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D016023:Integrins / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D038981:Receptors, Collagen
chemical_list: D016023:Integrins; D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; D003094:Collagen