Use of pulsed field gel electrophoresis to size the chromosome of the bacterial fish pathogen Yersinia ruckeri.
Field inversion gel electrophoresis (FIGE) and contour-clamped homogeneous field (CHEF) electrophoresis were used to analyse the chromosome of Yersinia ruckeri. The 8 base-pair recognition endonucleases, NotI and SfiI, generated less than 47 DNA fragments whose size and distribution were appropriate for pulsed field separation. Each isolate displayed a characteristic restriction pattern, with about 20% of bands in common. Depending on the strain used, the estimated genome size for this bacterial fish pathogen ranged from 4460 to 4770 kilobase pairs.
Romalde
J L
JL
Unité de Bactériologie Moléculaire et Medicale, Institut Pasteur, Paris, France.
Iteman
I
I
Carniel
E
E
D000818:Animals; D002876:Chromosomes, Bacterial / Q000378:metabolism / Q000648:ultrastructure*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005399:Fishes / Q000382:microbiology; D010957:Plasmids; D015007:Yersinia / Q000235:genetics*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes