Predominant rearrangements of the T cell receptor beta-chain gene in blood lymphocyte populations stimulated with autologous tumor cells suggest clonal T cell expansion in two of fourteen cases.
T cell responses against autologous tumors with samples from patients with a variety of tumors were examined. The abilities of T lymphocytes to lyse the autologous tumor cells were analyzed after short-term mixed lymphocyte/tumor cell cultures (MLTC). Southern blot analysis was used to evaluate whether particular rearrangements of the TCR beta-chain gene predominate in these cultures. Tumor specific lysis could be induced in a proportion of the mixed cultures. In two cases enrichment of T lymphocytes with similar TCR beta-chain gene rearrangements was detected after repeated stimulations with autologous tumor cells.
Stuber
G
G
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Vánky
F
F
Wen
T
T
Chan
A
A
Klein
E
E
Mak
T W
TW
D015139:Blotting, Southern; D003601:Cytotoxicity Tests, Immunologic; D004273:DNA, Neoplasm / Q000032:analysis; D005122:Exudates and Transudates; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
D004273:DNA, Neoplasm; D016693:Receptors, Antigen, T-Cell, alpha-beta