Simian sarcoma virus transformation of normal rat kidney fibroblasts is associated with markedly increased basic fibroblast growth factor expression.
Transformation of normal rat kidney fibroblasts (NRK) by the simian sarcoma virus (SSV) occurs as a result of expression of p28v-sis, a homologue of platelet-derived growth factor-B chain. Chromatographic separation revealed that the bulk (85%) of the mitogenic activity in SSV-transformed NRK cells was not due to p28v-sis but rather two distinct endothelial cell growth factors that eluted off heparin-Sepharose between 1 and 2 M NaCl. Protein purification and Northern blot analysis revealed that one of these growth factors was the 18 kd form of bFGF, the expression of which was found to increase 15-fold with SSV-transformation of NRK cells. The pure 18 Kd bFGF had no effect on NRK cell growth but was a potent neurotrophic agent for fetal rat cortical neurones and a potent growth factor for fetal bovine heart endothelial cells, suggesting a paracrine but not autocrine role for this protein. The second endothelial cell growth factor activity in SSV-transformed NRK cells was due to an 18 Kd protein which could be distinguished immunologically, biochemically, and mitogenically from bFGF.
Milner
P G
PG
Department of Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, MO 63110.
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002472:Cell Transformation, Viral; D002540:Cerebral Cortex / Q000187:drug effects; D002846:Chromatography, Affinity; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D005347:Fibroblasts / Q000382:microbiology; D015870:Gene Expression; D007668:Kidney / Q000382:microbiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009474:Neurons / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012842:Sarcoma Virus, Woolly Monkey / Q000235:genetics* / Q000378:metabolism
D015342:DNA Probes; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2