title: Quantitation of growth factors in ossein-mineral-compound.
abstract: Study was undertaken to identify polypeptide factors in the commercially available ossein-mineral-compound and to see if they are present in a biologically relevant quantity. Using the guanidine-EDTA extraction, 35.7 +/- 0.1 mg proteins were obtained from 1 g of the ossein-mineral-compound. At concentration 1 micrograms/ml, guanidine-EDTA-extractable proteins stimulated the incorporation of thymidine into DNA by human bone cells to 581 +/- 122% (p less than 0.001) of that by bovine serum albumin-treated control cells, decreasing thereafter. Similarly, it stimulated the activity of alkaline phosphatase in the human bone cells. Growth factors IGF-I, IGF-II, and TGF-beta were identified in the ossein-mineral-compound. This leads to speculation regarding possible role of growth factors in explaining the beneficial effects of the compound in retarding bone loss in patients with osteoporosis.
authors.affiliation: Department of Medicine, Loma Linda University, CA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Stepan
authors.forename: S
authors.initials: S
authors.lastname: Mohan
authors.forename: J C
authors.initials: JC
authors.lastname: Jennings
authors.forename: J E
authors.initials: JE
authors.lastname: Wergedal
authors.forename: A K
authors.initials: AK
authors.lastname: Taylor
authors.forename: D J
authors.initials: DJ
authors.lastname: Baylink
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D017886:Durapatite; D006133:Growth Substances / Q000032:analysis*; D006801:Humans; D006882:Hydroxyapatites / Q000032:analysis*; D066298:In Vitro Techniques; D010455:Peptides / Q000032:analysis*
chemical_list: D006133:Growth Substances; D006882:Hydroxyapatites; D010455:Peptides; D017886:Durapatite