Intercellular communication in cultured rabbit gastric epithelial cells.
The effects of drugs related to cyclic AMP and a tumor promoter, phorbol ester, on intercellular communications via gap junctions were investigated by the Lucifer Yellow-transfer method in cultured rabbit gastric epithelial cells. Cells were in contact with each drug for 4 hr before the microinjection of the dye into a cell. Dye transfer capacity was significantly increased by dibutyryl cyclic AMP (10(-3) M), theophylline (10(-3) M), 3-isobutyl-1-methylxanthine (10(-4) M), forskolin (10(-6) M) and irsogladine (10(-4) M); and it was inhibited by 12-O-tetradecanoyl-phorbol-13-acetate (100 ng/ml). These results suggest that the intercellular communication between cultured rabbit gastric epithelial cells is upregulated by cyclic AMP.
Ueda
F
F
Research Laboratories, Nippon Shinyaku Co. Ltd.
Kyoi
T
T
Mimura
K
K
Kimura
K
K
Yamamoto
M
M
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000032:analysis / Q000502:physiology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005110:Extracellular Space / Q000502:physiology; D005753:Gastric Mucosa / Q000737:chemistry / Q000378:metabolism; D007365:Intercellular Junctions / Q000187:drug effects / Q000502:physiology; D007546:Isoquinolines / Q000378:metabolism; D008854:Microscopy, Electron; D011817:Rabbits; D013270:Stomach / Q000166:cytology* / Q000187:drug effects; D014227:Triazines / Q000494:pharmacology; D015854:Up-Regulation / Q000502:physiology
D000897:Anti-Ulcer Agents; D007546:Isoquinolines; D014227:Triazines; C017475:lucifer yellow; D000242:Cyclic AMP; C041836:irsogladine