Cloning and sequence of an infectious bovine rhinotracheitis virus (BHV-1) gene homologous to glycoprotein H of herpes simplex virus.
A homologue to the glycoprotein H (gH) gene of herpes simplex virus (HSV) has been identified in the genome of infectious bovine rhinotracheitis virus (IBR, BHV-1). The gene is located immediately downstream from the thymidine kinase gene, and codes for an open reading frame (orf) of 842 amino acids. The orf has the characteristics of a membrane glycoprotein, including an N-terminal hydrophobic region resembling a signal sequence, a C-terminal region which is probably a transmembrane domain, and six potential sites for N-linked glycosylation. This orf shows significant homology to the gH sequences of both HSV and pseudorabies virus (PRV). We conclude that this gene encodes BHV-1 gH.
Meyer
A L
AL
Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.
Petrovskis
E A
EA
Duffus
W P
WP
Thomsen
D R
DR
Post
L E
LE
D001483:Base Sequence; D003001:Cloning, Molecular; D005814:Genes, Viral; D006031:Glycosylation; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D011558:Herpesvirus 1, Suid / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012689:Sequence Homology, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins; C044200:glycoprotein H, herpes simplex virus type 1