Detection of specific IgA antibodies against BK virus by ELISA.
The development and evaluation of an ELISA for analysis of anti-BKV specific IgA antibodies in human sera are described. All children with cancer with a primary BKV infection developed specific IgA antibodies, without any specific symptoms during the infection. Specific IgA was found in 61% of sera from healthy persons containing BKV IgG antibodies, using the chosen cut-off value, and BKV IgM in 4%. These results indicate that IgA production is more persistent than IgM. The high frequency of specific IgA antibodies could either be explained by frequent reactivations or long-lasting persistence of antibodies.
Nilsen
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I
Virological Research Group, University of Tromsö, Norway.
Flaegstad
T
T
Traavik
T
T
D000914:Antibodies, Viral / Q000032:analysis*; D001739:BK Virus / Q000276:immunology*; D001667:Binding, Competitive; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D007223:Infant; D007231:Infant, Newborn; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis*
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007075:Immunoglobulin M