[Determination of HIV-RNA using in situ hybridization on myocardial tissue. Role of HIV in cardiomyopathy in AIDS].
Aim of this study was to evaluate the presence of HIV-1 and CMV genomic sequences in myocardial tissue from autopsy of AIDS patients by in situ hybridization technique and to make a correlation with clinical and/or pathological signs of myocarditis. Seven patients out of 12 (58.3%) were positive for HIV-1 (6/7 patients with pathological signs of myocarditis and 1/5 without pathological abnormalities; p = 0.029). In situ hybridization for CMV was positive in 5/12 patients (41.7%) (3 with myocarditis and 2 without myocarditis); three clinically symptomatic cases were contemporary positive for HIV-1 and CMV. Our results seem to confirm emerging data about the presence of HIV-1 in myocardial tissue and about the possible role of HIV-1 in determining cardiac pathologies. Furthermore we suggest the hypothesis of an interaction of HIV-1 and CMV in latest of HIV infection.
Mencarini
P
P
Istituto di Clinica delle Malattie Infettive, Università Cattolica S. Cuore, Roma.
Tamburrini
E
E
Antinori
A
A
Zannoni
G
G
De Luca
A
A
Capelli
A
A
Ortona
L
L
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D000328:Adult; D003587:Cytomegalovirus / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006321:Heart / Q000382:microbiology*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009205:Myocarditis / Q000382:microbiology* / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D012307:Risk Factors
D004279:DNA, Viral
Ricerca di HIV-RNA mediante ibridizzazione in situ su tessuto miocardico. Ruolo dell'HIV nelle miocardiopatie in corso di AIDS.