title: Molecular and histological characterizations of the Om(2D) mutants in Drosophila ananassae.
abstract: A series of transposon-induced optic morphology (Om) mutants found in a hypermutable marker stock of Drosophila ananassae provides a useful system for analyzing the molecular mechanism of eye morphogenesis. In the present study, one of the 25 Om loci so far reported, Om(2D), has been subjected to histological and molecular analyses as a first step toward understanding the role of Om genes in eye morphogenesis. Histological abnormalities observed during eye morphogenesis of the mutant, i.e. cell death within the eye-antennal discs of third instar larvae, and loss of the lamina, disorganized ommatidia and atrophied optic lobes in adults, were all comparable to those reported with various eye morphology mutants of D. melanogaster. Approximately 25 kb of genomic DNA including the Om (2D) locus was cloned by tom tagging. Southern blot and cloning analyses of two alleles of the Om (2D) locus revealed that insertions of the tom element occurred at three sites within 359 bp; two tandemly arrayed toms sharing one long terminal repeat at the junction and an internally deleted tom were present 359 bp apart from each other in Om (2D) 63, while a single tom in reverse orientation was present within the 359 bp in Om (2D) 10a. Host DNA sequences at the three insertion sites were TATAT or AATAT, and ATAT was duplicated upon the tom insertion.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biology, Faculty of Science, Tokyo Metropolitan University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Matsubayashi
authors.forename: N
authors.initials: N
authors.lastname: Juni
authors.forename: K
authors.initials: K
authors.lastname: Usui
authors.forename: S H
authors.initials: SH
authors.lastname: Hori
authors.forename: Y N
authors.initials: YN
authors.lastname: Tobari
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003196:Computer Graphics; D004251:DNA Transposable Elements; D004330:Drosophila / Q000033:anatomy & histology / Q000235:genetics*; D005123:Eye / Q000254:growth & development; D007814:Larva; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009154:Mutation; D011679:Pupa; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements