Monoclonal antibodies toward a stable cyclic AMP-C8 antigen.
A cyclic AMP antigen was prepared utilizing a stable thioether linkage from C8 of the purine moiety. Monoclonal antibodies were raised toward the cyclic AMP-C8-diphtheria toxoid antigen and found to be of high specificity, with only very low cross-reactivity against related compounds, less than 0.03% against cGMP being the most significant cross-reaction. The stability of the thioether linkage enabled the preparation of an enzyme tracer, which was used in an ELISA. The assay had an effective working range from 0.1 to 100 pmol cAMP. Due to the structure of the antigen, the need for sample derivatization was eliminated.
Marcussen
J
J
Danisco A/S, Biotechnology Research Division, Copenhagen, Denmark.
Seiden
P
P
Olsen
C E
CE
van Onckelen
H
H
Rajagopal
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000737:chemistry / Q000276:immunology*; D004168:Diphtheria Toxoid / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D008099:Liver / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011685:Purine Nucleotides / Q000737:chemistry; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000941:Antigens; D004168:Diphtheria Toxoid; D011685:Purine Nucleotides; D000242:Cyclic AMP