Regional mapping of 22 microclones around the adenomatous polyposis coli (APC) locus on chromosome 5q.
A previously described genomic library constructed from microdissected DNA has been used to generate a large number of probes around the adenomatous polyposis coli (APC) gene at 5q22. A total of 202 clones were hybridised directly onto a somatic cell hybrid panel containing two APC-related interstitial deletions. Of 75 microclones that gave clear hybridisation signals, 22 independent clones mapped into the region common to both deletions. In addition, 4/22 of the markers are conserved in rodent DNA. These clones should provide a valuable resource for screening cDNA libraries and cloning the DNA around the APC gene in yeast artificial chromosomes.
Hampton
G M
GM
Director's Laboratory, Imperial Cancer Research Fund, London, UK.
Howe
C
C
Leuteritz
G
G
Thomas
H
H
Bodmer
W F
WF
Solomon
E
E
Ballhausen
W G
WG
D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D015698:Genomic Library; D006801:Humans