A comparison of Epstein-Barr virus specific proteins expressed by three Epstein-Barr virus isolates using specific monoclonal antibodies.
Monoclonal antibodies (MAbs) were prepared in mice inoculated with a nasopharyngeal carcinoma Epstein-Barr virus (NPC-EBV) producer lymphoblastoid cell line (NPC-LC). When the 214A9 MAb was used to study the expression of the 52/50-kD early antigen in NPC-LC, B95-8, and HR-1 cells, no difference was found. The same results were obtained with the 343D12 MAb which is to a 125-kD virus capsid antigen protein. However, the 244G11 MAb detected an EBV membrane antigen (MA) with a molecular weight of 220 kD in both B95-8 and HR-1 cells, but reacted with a 250-kD MA protein in the NPC-LC cells. Similar results were obtained with the 201D6 MAb which reacted with a MA polypeptide with a molecular weight of 220/200 kD in HR-1 and B95-8 cells and one of 250/200 kD in NPC-LC cells. The data suggest that there may be differences in at least two MA proteins synthesized by the NPC-EBV isolate as compared with the prototype HR-1 and B95-8 EBV isolates.
Tsai
C H
CH
Department of Medical Microbiology and Immunology, Ohio State University Medical Center, Columbus 43210.
Glaser
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000378:metabolism; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D013045:Species Specificity; D014764:Viral Proteins / Q000096:biosynthesis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D014764:Viral Proteins