title: Expression of a biologically active ovine trophoblastic interferon using a baculovirus expression system.
abstract: Ovine trophoblast protein (oTP) an embryonic interferon, which plays a key role in maternal recognition of pregnancy, has been expressed in insect cells using a baculovirus expression system. A cDNA coding for oTP was inserted downstream of the strong polyhedrin promoter. Cells infected with recombinant virus produced biologically active oTP and greater than 90% was secreted into the culture medium during infection. High amount of antiviral activity were produced (up to 5 x 10(5) IU per ml of culture medium). Recombinant oTP (roTP) was purified by immunoaffinity chromatography and found to be identical to authentic oTP with respect to molecular mass and N-terminal amino acid sequence.
authors.affiliation: Station de Recherches de Pathologie Comparée, URA CNRS n. 11840, I.N.R.A., Saint-Christol-lez-Alès, France.
authors.forename: M
authors.initials: M
authors.lastname: Cerutti
authors.forename: D
authors.initials: D
authors.lastname: Hue
authors.forename: M
authors.initials: M
authors.lastname: Charlier
authors.forename: R
authors.initials: R
authors.lastname: L'Haridon
authors.forename: J C
authors.initials: JC
authors.lastname: Pernollet
authors.forename: G
authors.initials: G
authors.lastname: Devauchelle
authors.forename: P
authors.initials: P
authors.lastname: Gaye
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D002846:Chromatography, Affinity; D015151:Immunoblotting; D007313:Insecta; D007370:Interferon Type I / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011257:Pregnancy Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D015183:Restriction Mapping; D012756:Sheep; D014162:Transfection; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D007370:Interferon Type I; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; C020947:interferon tau