Fructose catabolism in Xanthomonas campestris pv. campestris. Sequence of the PTS operon, characterization of the fructose-specific enzymes.
In Xanthomonas campestris pv. campestris, fructose is transported and phosphorylated into fructose 1-phosphate through a phosphoenolpyruvate-dependent phosphotransferase system. The nucleotide sequence of the fruA gene encoding the phosphotransferase system permease specific of fructose (EIIFru) was determined. The fructose 1-phosphate produced by the phosphotransferase system is phosphorylated into fructose 1,6-bisphosphate by a 1-phosphofructokinase. This enzyme was characterized and the corresponding gene (fruK) was sequenced. Sequence comparisons revealed that FruK is a member of a new family of ATP-binding proteins composed of sugar (or sugar-phosphate) kinases. In phosphotransferase system-deficient strains, fructose can still be transported by an unidentified permease. The intracellular fructose is then phosphorylated by a multimeric fructokinase of 135 kDa specific for fructose and inhibited by fructose, fructose 1,6-bisphosphate, and mannose. Several other enzymes of fructose metabolism were assayed and a potential pathway for fructose catabolism is presented.
de Crécy-Lagard
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Unité de Régulation de l'Expression Génétique, Institut National de la Santé et de la Recherche Médicale Unité 199, Institut Pasteur, Paris, France.
Bouvet
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OM
Lejeune
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Danchin
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A
D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005632:Fructose / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009876:Operon; D010732:Phosphofructokinase-1 / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics*; D015183:Restriction Mapping; D016354:Rhodobacter capsulatus / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014974:Xanthomonas / Q000378:metabolism*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005632:Fructose; D010770:Phosphotransferases; D010732:Phosphofructokinase-1; C068652:1-phosphofructokinase