Variation in thickness of the large cryosections cut for whole-body autoradiography.
A method to assess variation in thickness of the large cryosections for whole-body autoradiography (WBARG) was described, and the degree of intraslice and interslice variations were determined for our cryomicrotome system (LKB PMV-2250). Intraslice variation in thickness of the 180 x 80 mm cryosection was 0.72-0.92 micron within the range of section thickness for WBARG (15-50 microns), and interslice variation was 0.89-1.21 microns. These potential variations in section thickness should be kept in mind whenever working with quantitative WBARG.
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Department of Nuclear Medicine, University of Massachusetts Medical Center, Worcester 01655.
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D000818:Animals; D001345:Autoradiography / Q000295:instrumentation / Q000379:methods*; D003201:Computers; D007457:Iodine Radioisotopes; D008099:Liver / Q000000981:diagnostic imaging*; D011859:Radiography
D007457:Iodine Radioisotopes