title: Solid scintillators for receptor assays: an environmentally safe alternative to liquid scintillation cocktails.
abstract: Scintillation counting of tritiated ligands is widespread in receptor assays and has necessitated the use of scintillation cocktails containing environmentally damaging solvents that pose health hazards to their users. A safer mode of dry scintillation counting, based on the solid scintillator Xtalscint, was evaluated in whole-cell and membrane receptor assays. The results compared favorably with those obtained with glass-fiber filters and conventional liquid counting methods. It is concluded that solid scintillators may be used as an environmentally safer alternative to liquid scintillation in these assays.
authors.affiliation: Donald L. Horrocks Nuclear Applications Laboratory, Beckman Instruments, Inc., Fullerton, CA 92634.
authors.forename: E F
authors.initials: EF
authors.lastname: Hawkins
mesh_terms: D000042:Absorption; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004787:Environmental Pollution / Q000517:prevention & control*; D006801:Humans; D011850:Radioactive Waste / Q000517:prevention & control*; D011869:Radioligand Assay / Q000379:methods*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D011954:Receptors, Dopamine / Q000378:metabolism; D017447:Receptors, Dopamine D1; D012588:Scintillation Counting / Q000379:methods*
chemical_list: D011850:Radioactive Waste; D011943:Receptors, Adrenergic, beta; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1