N.m.r. and conformational analysis of the capsular polysaccharide from Streptococcus pneumoniae type 4.
The 1H- and 13C-n.m.r. data on the capsular polysaccharide (1) produced by Streptococcus pneumoniae type 4, the depyruvated polysaccharide (2), and a tetrasaccharide (3a) derived by Smith degradation of 2 were used as constraints on a computer-generated model of the conformation of 1 and to assess the effects of the pyruvic acetal substituent on the conformation. The dynamics of the polysaccharide systems and the influence of the pyruvic acetal were investigated using 13C-n.m.r. relaxation measurements.
Jones
C
C
Laboratory of Molecular Structure, National Institute for Biological Standards and Control, South Mimms, Herts., Great Britain.
Currie
F
F
Forster
M J
MJ
D000577:Amides / Q000737:chemistry; D016667:Bacterial Capsules / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D003198:Computer Simulation; D006358:Hot Temperature; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011522:Protons; D011773:Pyruvates / Q000737:chemistry; D013296:Streptococcus pneumoniae / Q000737:chemistry*
D000577:Amides; D002247:Carbon Isotopes; D011135:Polysaccharides, Bacterial; D011522:Protons; D011773:Pyruvates; C064986:pyruvic acid acetal