Protective effect of gangliosides on myocardial hypoxic damage in the rat.
The size of the infarct produced by ligation of the left coronary artery in the rat was decreased significantly in animals treated i.p. with 40 mg/kg per day of a ganglioside mixture (GMIX) for 7 days after surgery. Rats treated with GMIX had lower ventricular myeloperoxidase activity, indicating a lower leukocyte infiltration after infarction. The underperfused zone was also smaller in animals treated daily with GMIX 30 days after surgery. Control hearts, but not hearts obtained from animals pretreated for 15 days with 40 mg/kg per day of GMIX, released lactate dehydrogenase (LDH) during perfusion in a Langerdorff apparatus after ligation and reperfusion of the left coronary artery in vitro. Hearts made hypoxic in vitro by changing the perfusion gas to nitrogen for 20 min and later reoxygenating with 95% O2 -5% CO2 released LDH in the perfusate, but did not do so in the presence of 10 microM monosialotetraesosylganglioside. Gangliosides, therefore, seem to protect the rat heart against hypoxic damage.
Dolara
P
P
Department of Pharmacology and Toxicology, University of Florence, Italy.
Lodovici
M
M
Fazi
M
M
Lasagni
L
L
Sturlini
S
S
D000818:Animals; D009202:Cardiomyopathies / Q000209:etiology / Q000517:prevention & control*; D003331:Coronary Vessels / Q000502:physiology; D005732:Gangliosides / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D000860:Hypoxia / Q000150:complications*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D009203:Myocardial Infarction / Q000503:physiopathology / Q000517:prevention & control; D009206:Myocardium / Q000201:enzymology; D009584:Nitrogen; D010477:Perfusion; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D005732:Gangliosides; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase; D009584:Nitrogen