title: Left ventricular beta 1 and beta 2 adrenoceptor mRNA expression in normal and volume overloaded human heart.
abstract: OBJECTIVE
  The aim was to determine gene expressions of beta 1 and beta 2 adrenoceptor subtypes (BAR-1, BAR-2) in normal and volume overloaded human heart.
  
  
  METHODS
  Tissue mRNA levels were determined by excess solution hybridisation using 35S-UTP labelled BAR-1 and BAR-2 cRNA probes.
  
  
  METHODS
  Atrium, right and left ventricular subendocardium, and papillary muscle from donor hearts and papillary muscle from patients operated for mitral stenosis and mitral regurgitation were studied.
  
  
  RESULTS
  The basal levels of myocardial BAR-1 and BAR-2 mRNA expression were similar to the levels in human adipose tissue that have been reported previously from our laboratory (10-15 amol mRNA.micrograms-1 total nucleic acids). No differences in BAR-1 and BAR-2 mRNA expression were observed between various parts of the normal heart. In papillary muscle, BAR-1 and BAR-2 mRNA levels were 8.8 (SEM 5.1) and 10.2(6.6) amol.micrograms-1 total nucleic acids, respectively. Furthermore, no differences in BAR mRNA expression were observed between myocardium subjected to mitral stenosis as compared to normal myocardium. On the other hand, patients with mitral regurgitation expressed significantly lower levels of both BAR-1 and BAR-2 mRNA, at 4.8(1.0) (p less than 0.05) and 2.6(1.1) (p less than 0.001) amol.micrograms-1 total nucleic acids, respectively. The ratio BAR-1/BAR-2 mRNA was higher (p less than 0.01) in mitral regurgitation than in the normal heart.
  
  
  CONCLUSIONS
  Myocardium from different parts of the normal human heart, where different pressure work is generated, express similar levels of BAR-1 and BAR-2 mRNA. With volume load a significant decrease in BAR mRNA levels was observed, which was more marked for BAR-2 mRNA. This difference in specific mRNA levels in patients with mitral regurgitation indicates an independent regulation of the expression of these two receptor subtypes.
authors.affiliation: Department of Medicine, Huddinge Hospital, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Sylvén
authors.forename: P
authors.initials: P
authors.lastname: Arner
authors.forename: L
authors.initials: L
authors.lastname: Hellström
authors.forename: E
authors.initials: E
authors.lastname: Jansson
authors.forename: P
authors.initials: P
authors.lastname: Sotonyi
authors.forename: A
authors.initials: A
authors.lastname: Somogyi
authors.forename: M
authors.initials: M
authors.lastname: Brönnegård
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D008875:Middle Aged; D008946:Mitral Valve Stenosis / Q000235:genetics*; D015336:Molecular Probe Techniques; D009206:Myocardium / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D011943:Receptors, Adrenergic, beta / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta