Cell type determines the relative proportions of (-) and (+) strand RNA during poliovirus replication.
To examine the influence of the host cell type on poliovirus RNA synthesis we compared the levels of (-) and (+) strand poliovirus RNA during infection of epithelial (HeLa and HEp-2), leukocytic (U-937, HL-60 and K-562) and nerve (IMR-32) cells. The levels of (-) strand RNA were higher in IMR-32, U-937, K-562 or HL-60 cells than those in HeLa or HEp-2 cells. By contrast, (+) strand RNA content was greater in HeLa or HEp-2 cells. Although significant levels of (+) strand RNA were detected in U-937, K-562 and HL-60 cells, no viral protein synthesis was detected by polyacrylamide gel electrophoretic analysis of metabolically labelled proteins. The molar ratio of poliovirus (-) and (+) RNAs was 2-3 fold higher in IMR-32, U-937 and K-562 cells than in HeLa or HL-60 cells and 5-6 fold higher than in HEp-2 cells. Differentiation of HL-60 cells with a variety of inducers produced differential effects on poliovirus (-) and (+) RNA content and modified the molar ratio of (-)/(+) strand RNAs. These findings indicate that host cell components play a critical role in the regulation of the amount of poliovirus (-) and (+) strand RNAs synthesized during infection.
López-Guerrero
J A
JA
Centro de Biología Molecular, Universidad Autonóma, CSIC, Madrid, Spain.
Martínez-Abarca
F
F
Fresno
M
M
Carrasco
L
L
Alonso
M A
MA
D002460:Cell Line; D006801:Humans; D007700:Kinetics; D017955:Poliovirus / Q000502:physiology*; D016372:RNA, Antisense / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D014779:Virus Replication / Q000502:physiology*
D016372:RNA, Antisense; D012367:RNA, Viral