title: Interaction of lambda Gam protein with the RecD subunit of RecBCD enzyme increases radioresistance of the wild-type Escherichia coli.
abstract: By making use of the gam(+)-plasmid, the so-called gam-dependent radioresistance was studied. This resistance is the result of the interaction between Gam protein (encoded by the gam gene of lambda) and RecBCD enzyme of Escherichia coli. gam-dependent radioresistance is observed in recB+ recC+ recD+ but not in recB+ recC+ recD- cells. It is suggested that Gam protein interacts specifically with the RecD subunit of RecBCD enzyme; the RecBC complex probably retains its activity in the presence of this viral protein.
authors.affiliation: Ruder Bosković Institute, Zagreb, Yugoslavia.
authors.forename: N
authors.initials: N
authors.lastname: Marsić
authors.forename: E
authors.initials: E
authors.lastname: Salaj-Smic
authors.forename: I
authors.initials: I
authors.lastname: Stojiljković
authors.forename: Z
authors.initials: Z
authors.lastname: Trgovcević
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics* / Q000528:radiation effects; D004265:DNA Helicases / Q000235:genetics* / Q000528:radiation effects; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects*; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics* / Q000528:radiation effects; D005720:Gamma Rays; D010957:Plasmids; D014764:Viral Proteins / Q000235:genetics* / Q000528:radiation effects
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D014764:Viral Proteins; C065410:gam protein, Coliphage; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli; D004265:DNA Helicases