Production of human interferon-beta by Sendai virus and poly(rI).poly(rC): inhibition by neomycin.
Neomycin inhibits the production of interferon-beta (IFN-beta) in human fibroblast cells in response to Sendai virus or to poly(rI).poly(rC) in a concentration-dependent manner, and to the greatest extent effective when added prior to or up to 2 h after induction. This inhibitory effect is negated when the protein kinase C activator, SC-9, is present during IFN-beta production in response to poly(rI).poly(rC), but not in response to Sendai virus. These results suggest that in human cells both virus and poly(rI).poly(rC) utilize an early neomycin-sensitive signal transduction step for the production of IFN-beta; because neomycin binds specific phosphatidylinositol phosphates, both of these inducers very likely require hydrolysis of these phosphates.
Lin
H Y
HY
Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York, Buffalo.
Davis
P J
PJ
Thacore
H R
HR
D002460:Cell Line; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000382:microbiology; D006801:Humans; D016899:Interferon-beta / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D009355:Neomycin / Q000494:pharmacology*; D010222:Parainfluenza Virus 1, Human / Q000187:drug effects* / Q000254:growth & development; D011070:Poly I-C / Q000494:pharmacology*; D015398:Signal Transduction; D013449:Sulfonamides / Q000494:pharmacology
D013449:Sulfonamides; C049999:N-(6-phenylhexyl)-5-chloro-1-naphthalenesulfonamide; D016899:Interferon-beta; D009355:Neomycin; D011070:Poly I-C