title: Nitric oxide as an antioxidant.
abstract: Benzoate monohydroxy compounds, and in particular salicylate, were produced during interaction of ferrous complexes with hydrogen peroxide (Fenton reaction) in a N2 environment. These reactions were inhibited when Fe complexes were flushed, prior to the addition in the model system, by nitric oxide. Methionine oxidation to ethylene by Fenton reagents was also inhibited by nitric oxide. Myoglobin in several forms such as metmyoglobin, oxymyoglobin, and nitric oxide-myoglobin were interacted with an equimolar concentration of hydrogen peroxide. Spectra changes in the visible region and the changes in membrane (microsomes) lipid peroxidation by the accumulation of thiobarbituric acid-reactive substances (TBA-RS) were determined. The results showed that metmyoglobin and oxymyoglobin were activated by H2O2 to ferryl myoglobin, which initiates membrane lipid peroxidation; but not nitric oxide-myoglobin, which, during interaction with H2O2, did not form ferryl but metmyoglobin which only poorly affected lipid peroxidation. It is assumed that nitric oxide, liganded to ferrous complexes, acts to prevent the prooxidative reaction of these complexes with H2O2.
authors.affiliation: Department of Food Science, Volcani Center, Bet Dagan, Israel.
authors.forename: J
authors.initials: J
authors.lastname: Kanner
authors.forename: S
authors.initials: S
authors.lastname: Harel
authors.forename: R
authors.initials: R
authors.lastname: Granit
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000378:metabolism; D001565:Benzoates / Q000378:metabolism; D019817:Benzoic Acid; D005296:Ferrous Compounds / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D006878:Hydroxides / Q000378:metabolism; D017665:Hydroxyl Radical; D006900:Hydroxylation; D008715:Methionine / Q000378:metabolism; D008786:Metmyoglobin / Q000378:metabolism; D009211:Myoglobin / Q000378:metabolism; D009569:Nitric Oxide / Q000494:pharmacology*; D012459:Salicylates / Q000378:metabolism; D020156:Salicylic Acid; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D001565:Benzoates; D005296:Ferrous Compounds; D006878:Hydroxides; D009211:Myoglobin; D012459:Salicylates; C017595:oxymyoglobin; D008786:Metmyoglobin; D009569:Nitric Oxide; D017665:Hydroxyl Radical; C020748:ferrous sulfate; D019817:Benzoic Acid; D008715:Methionine; D006861:Hydrogen Peroxide; D020156:Salicylic Acid; D001205:Ascorbic Acid