title: Inhibition of Streptococcus mutans adherence to hydroxyapatite with combinations of alkyl phosphates and nonionic surfactants.
abstract: Seven alkyl phosphate derivatives and three nonionic surfactants were examined for their ability to inhibit the adherence of 3H-labelled cells of Streptococcus mutans to hydroxyapatite (HA) treated with buffer (BHA) or parotid saliva (SHA). None of the compounds alone hindered binding of bacteria to BHA or SHA effectively. A combination of certain of the alkyl phosphates, notably a disodium phosphate of 1-octadecanol, and nonionic surfactant at a 1:1 molar ratio gave a strong inhibition of S. mutans adherence. Treatment of SHA with this combination resulted in 98% reduction of adherence. Adsorption of the two types of surface-active agents alone and in combinations was studied using 14C-labelled agents. Electrophoretic measurements revealed that surfaces treated with only alkyl phosphate became highly negatively charged, while surfaces treated with the combination had a zeta-potential of around zero. The results suggest that treatment with surfactants might be used to interfere with bacterial adherence on the tooth surface.
authors.affiliation: Department of Cariology, Faculty of Odontology, University of Göteborg, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Olsson
authors.forename: A
authors.initials: A
authors.lastname: Carlén
authors.forename: K
authors.initials: K
authors.lastname: Holmberg
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D003773:Dental Plaque / Q000517:prevention & control; D017886:Durapatite; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D010710:Phosphates / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry; D011188:Potassium / Q000494:pharmacology*; D017680:Potassium Compounds; D012463:Saliva / Q000502:physiology; D013295:Streptococcus mutans / Q000502:physiology*; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D006882:Hydroxyapatites; D010710:Phosphates; D017680:Potassium Compounds; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017886:Durapatite; C013216:potassium phosphate; D011188:Potassium; C018279:sodium phosphate