title: Which 3-ribofuranosyl-substituted purine 5'-phosphates undergo template-directed oligomerization?
abstract: We have studied the oligomerization reactions of the 2-methylimidazolide derivatives of 3-isoisoguanosine 5'-phosphate (2) and 3-isoxanthosine 5'-phosphate (5) in the presence of a variety of homopolynucleotide templates. In no case did we observe a substantial template-facilitated production of long oligomers. Polyuridylic acid directed the synthesis of low molecular-weight products from both monomers. Polycytidylic acid, polyadenylic acid, polyinosinic acid, and polyguanylic acid were ineffective as templates in the systems that we investigated.
authors.affiliation: Salk Institute for Biological Studies, San Diego, CA 92186-5800.
authors.forename: A R
authors.initials: AR
authors.lastname: Hill
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: V D
authors.initials: VD
authors.lastname: Patil
authors.forename: N J
authors.initials: NJ
authors.lastname: Leonard
authors.forename: L E
authors.initials: LE
authors.lastname: Orgel
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives* / Q000235:genetics; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives / Q000138:chemical synthesis; D012263:Ribonucleosides / Q000138:chemical synthesis; D013698:Templates, Genetic
chemical_list: D012263:Ribonucleosides; C070260:3-isoisoguanosine 5'-phospho-2-methylimidazole; C070259:3-ribofuranosylisoguanosine 5'-diphosphate; C070261:3-isoxanthosine 5'-phospho-2-methylimidazole; D006153:Guanosine Diphosphate; D006157:Guanosine Monophosphate