Characterization of BPV-like DNA in equine sarcoids.
The DNA from equine sarcoid samples from New York State and Switzerland was isolated and probed with bovine papillomavirus type 1 (BPV-1) to determine if BPV genomes were present. Twelve of 13 sarcoids from New York State and 17/20 sarcoids from Switzerland contained DNA that hybridized to the BPV-1 probe. Restriction enzyme analysis of the positive samples demonstrated restriction fragment profiles characteristic of BPV-1 in 22 sarcoids and restriction fragment profiles characteristic of bovine papillomavirus type 2 (BPV-2) in 7 sarcoids. In addition, three tissues histologically diagnosed as pyogranulomatous dermatitis, fibropapilloma, and fibrosarcoma contained BPV-like DNA. Tissues with BPV-1-like and BPV-2-like DNA contained an average of 285.7 (21 to 808) and 125.8 (2 to 762) BPV-like genomes per cell, respectively. Minor differences in the restriction fragment profiles of the BPV-like DNA and evidence for partial BPV-like genomes were found in some sarcoids. BPV-like DNA was not detected in lymphocyte DNA from sarcoid-affected horses. These results confirm previous observations and support the hypothesis that bovine papillomavirus, or a very similar virus, is linked to the cause of equine sarcoid.
Angelos
J A
JA
James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca.
Marti
E
E
Lazary
S
S
Carmichael
L E
LE
D000818:Animals; D015139:Blotting, Southern / Q000662:veterinary; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000302:isolation & purification*; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D012507:Sarcoidosis / Q000382:microbiology / Q000662:veterinary*; D012871:Skin Diseases / Q000382:microbiology / Q000662:veterinary*
D015342:DNA Probes; D004279:DNA, Viral; D004262:DNA Restriction Enzymes