Calmodulin-like protein and the phospholipids of Mycobacterium smegmatis.
When Mycobacterium smegmatis TMC1546 was grown at different concentrations of glucose supplemented to a synthetic medium already containing 2% v/v glycerol, the following changes were observed. Amount of calmodulin-like protein (CAMLP), total and individual phospholipids (PLs) namely phosphatidylethanolamine, cardiolipin, phosphatidylglycerol and phosphatidylinositol mannosides and total lipids and growth increased up to 5% w/v but decreased at higher concentrations of glucose (7.5% w/v and above). Cyclic AMP content of the whole cells decreased continuously with increase in glucose concentration in the medium. Incorporation of 32Pi into total phospholipids was inhibited by two calmodulin antagonists trifluoperazine and phenothiazine (50% at 40 microM) and the calcium-specific chelator ethylene glycol bis (beta-aminoethyl ether) N,N,N',N'-tetraacetate (EGTA) 35% at 2 mM. Total lipids, CAMLP and growth of this organism are also modulated in a similar way in response to the glucose concentration in the growth medium. Taking these observations together it is suggested that CAMLP has some effect on the metabolism of PLs.
Burra
S S
SS
Department of Biochemistry, University College of Medical Sciences, Shahdara, Delhi, India.
Reddy
P H
PH
Falah
S M
SM
Venkitasubramanian
T A
TA
Murthy
P S
PS
D002118:Calcium / Q000502:physiology; D002147:Calmodulin / Q000032:analysis* / Q000502:physiology; D000242:Cyclic AMP / Q000032:analysis; D005947:Glucose / Q000494:pharmacology; D009161:Mycobacterium / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis* / Q000378:metabolism
D002147:Calmodulin; D010743:Phospholipids; D000242:Cyclic AMP; D005947:Glucose; D002118:Calcium