title: In vitro cytopathogenicity and in vivo virulence of two strains of canine parainfluenza virus.
abstract: In vivo and in vitro properties of two strains of canine parainfluenza virus (CPIV) were investigated. One strain, designated CPIV(+), induced syncytial giant cell formation and cytolysis in vitro, whereas the second strain, CPIV(-), caused only a mild strand-forming cytopathic effect with few, small syncytial giant cells. Vero cells infected with CPIV(+) or CPIV(-) were 100% positive for CPIV antigen as determined by immunofluorescent staining; however, 100% of CPIV(+) and less than 10% of CPIV(-) infected cells were hemadsorption positive. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis revealed no differences in electrophoretic mobility of viral polypeptides between both strains; however, in CPIV(-), reduced or absent synthesis of the putative HN and F1 proteins was observed. Isopycnic separation of CPIV(+) progeny virions showed a high proportion of viral particles with a buoyant density of 1.18 g/cm3. In contrast, CPIV(-) progeny virions had a heterogeneous density profile ranging from 1.08 to 1.18 g/cm3. Intracerebral infection of six ferrets with CPIV(+) resulted in moderate lymphocytic and histiocytic choroiditis, meningitis, and ependymitis, whereas CPIV(-) infection caused only mild to moderate inflammation. Immunohistologically, CPIV antigen was prominent in ependymal lining cells of the ventricles in CPIV(+)-infected ferrets and was reduced or lacking in CPIV(-)-infected ferrets (n = 6). Sham-injected ferrets (n = 6) did not have histologic lesions and no viral antigen was identified. The present findings suggest that certain changes in the activities of CPIV glycoproteins may lead to alterations of CPIV virulence in vivo.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Ohio State University, Columbus.
authors.forename: W
authors.initials: W
authors.lastname: Baumgärtner
authors.forename: S
authors.initials: S
authors.lastname: Krakowka
authors.forename: B
authors.initials: B
authors.lastname: Durchfeld
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain / Q000382:microbiology / Q000473:pathology; D002499:Centrifugation, Density Gradient; D003588:Cytopathogenic Effect, Viral; D004283:Dog Diseases / Q000382:microbiology* / Q000473:pathology; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004805:Ependyma / Q000382:microbiology / Q000473:pathology; D005289:Ferrets; D005455:Fluorescent Antibody Technique; D015726:Giant Cells; D007150:Immunohistochemistry; D018184:Paramyxoviridae Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D018108:Respirovirus / Q000276:immunology / Q000472:pathogenicity*; D014709:Vero Cells; D014774:Virulence
chemical_list: D000956:Antigens, Viral