title: Molecular analysis of tap2, an anther-specific gene from Antirrhinum majus.
abstract: Deficiens is a floral homeotic gene of Antirrhinum majus, mutation of which results in transformation of petals to sepals and stamens to carpels. In a search for putative target genes, controlled by this regulatory locus, cDNA clones representing genes, that are expressed in wild type but not in the deficiens mutant flowers, were isolated by differential screening. The molecular structure and the expression pattern of one of these genes, tap2, is described. Tap2 is transiently and tissue-specifically expressed in the tapetum of the anthers. It encodes a 131 amino-acids-long protein with a hydrophobic N-terminus, displaying all characteristic features of a signal peptide. This indicates that the TAP2 protein may be secreted.
authors.affiliation: Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
authors.forename: W K
authors.initials: WK
authors.lastname: Nacken
authors.forename: P
authors.initials: P
authors.lastname: Huijser
authors.forename: H
authors.initials: H
authors.lastname: Saedler
authors.forename: H
authors.initials: H
authors.lastname: Sommer
mesh_terms: D000595:Amino Acid Sequence; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D015870:Gene Expression; D005801:Genes, Homeobox; D015698:Genomic Library; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D016372:RNA, Antisense / Q000032:analysis; D016385:TATA Box; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D021382:Protein Sorting Signals; D016372:RNA, Antisense; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D004247:DNA