title: Measurement of aromatisation by a urine technique suitable for the evaluation of aromatase inhibitors in vivo.
abstract: By modification of a recently developed method for separation of radio-labelled urinary oestrogens we were able to separate oestrogen metabolites and measure their isotope ratios in urine following injections of [3H]delta 4-androstenedione and [14C]oestrone. This method provides a useful tool for studying in vivo aromatisation of delta 4-androstenedione into oestrone in breast cancer patients before and during treatment with aromatase inhibitors.
authors.affiliation: Department of Academic Biochemistry, Royal Marsden Hospital, London, England.
authors.forename: S
authors.initials: S
authors.lastname: Jacobs
authors.forename: P E
authors.initials: PE
authors.lastname: Lønning
authors.forename: B
authors.initials: B
authors.lastname: Haynes
authors.forename: L
authors.initials: L
authors.lastname: Griggs
authors.forename: M
authors.initials: M
authors.lastname: Dowsett
mesh_terms: D000616:Aminoglutethimide / Q000627:therapeutic use; D000735:Androstenedione / Q000378:metabolism; D001141:Aromatase / Q000378:metabolism*; D047072:Aromatase Inhibitors; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D004967:Estrogens / Q000652:urine*; D004970:Estrone / Q000096:biosynthesis; D005260:Female; D006801:Humans
chemical_list: D047072:Aromatase Inhibitors; D004967:Estrogens; D000616:Aminoglutethimide; D004970:Estrone; D000735:Androstenedione; D001141:Aromatase