Isolation of equine peripheral blood mononuclear cells using Percoll.
The concentration of Percoll required for isolating equine peripheral blood mononuclear cells has been reinvestigated. A poor cell yield was obtained at the 60 per cent concentration already reported. It is recommended that workers specifically interested in high yields of mononuclear cells, for investigation of lymphocyte and monocyte functions, use a concentration of 65 per cent Percoll. However, workers wishing to isolate pure populations of equine neutrophils might consider a concentration of 70 per cent in the upper layer of Percoll used to retain the mononuclear cells.
May
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Royal Veterinary College, North Mymms, Hatfield, Hertfordshire.
Hooke
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Lees
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D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D006736:Horses / Q000097:blood*; D007963:Leukocytes, Mononuclear; D011205:Povidone; D012822:Silicon Dioxide
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone