Quantitation of l-epinephrine and determination of the d-/l-epinephrine enantiomer ratio in a pharmaceutical formulation by capillary electrophoresis.
A procedure for the quantitation of l-epinephrine and the determination of the d-/l-epinephrine ratio in a pharmaceutical formulation containing l-epinephrine is described. The optical isomers of epinephrine were resolved by capillary electrophoresis with a buffer containing heptakis-(2,6-di-o-methyl)-beta-cyclodextrin. Quantitation was achieved with the use of an internal standard, 1-pseudoephedrine. Results with and without internal standard correction illustrate the improved reproducibility possible with an internal standard.
Peterson
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Alcon Laboratories, Inc., Fort Worth, TX 76134-2099.
Trowbridge
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D004586:Electrophoresis / Q000379:methods; D004837:Epinephrine / Q000032:analysis*; D009883:Ophthalmic Solutions; D015203:Reproducibility of Results; D013237:Stereoisomerism
D009883:Ophthalmic Solutions; D004837:Epinephrine