title: [Comparison of immunofluorescence reagents for the detection of the early antigen of cytomegaloviruses in the Shell-vial].
abstract: Detection of cytomegalovirus has been carried out in 178 clinical samples in order to compare the effectiveness of two distinct commercial immunofluorescence reagents: anti-CMV early nuclear protein (Du Pont) and CMV culture identification (Syva Micro-Trak). In 61 of the samples we observed positive reaction to either of the two procedures (Shell-vial and, or, cellular conventional culture). Sensitivity was 0.92 for Du Pont reagent, 0.79 for Syva reagent, and 0.93 for the conventional cellular culture. In the overall samples we observed a higher incidence of fluorescent focus using Du Pont reagent that using that of Syva.
authors.affiliation: Servicio de Microbiología, Ciudad Sanitaria Virgen de las Nieves, Granada.
authors.forename: J
authors.initials: J
authors.lastname: Mendoza Montero
authors.forename: J M
authors.initials: JM
authors.lastname: Navarro Marí
authors.forename: A
authors.initials: A
authors.lastname: Rojas González
authors.forename: J C
authors.initials: JC
authors.lastname: Plata Rosales
authors.forename: M
authors.initials: M
authors.lastname: de la Rosa Fraile
mesh_terms: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006801:Humans; D017874:Immediate-Early Proteins; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D014776:Virus Cultivation
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D011933:Reagent Kits, Diagnostic; C044582:immediate-early proteins, cytomegalovirus
vernacular_title: Comparación de reactivos de inmunofluorescencia para la detección de antígeno precoz de citomegalovirus en Shell-vial.