title: Dopamine D1 receptor stimulation of cyclic AMP accumulation in COS-1 cells.
abstract: Dopamine is shown to stimulate cAMP accumulation in COS-1 cells via endogenously expressed dopamine D1 receptors. A dissociation of dopamine and beta-adrenoceptor responses is demonstrated by the use of selective antagonists and different desensitization patterns following exposure of the cells to dopamine or the beta-adrenoceptor agonist, isoproterenol. The dopamine response in COS-1 cells exhibits a pharmacological profile similar to that found in dopamine D1 tissues such as rat striatum and fish retina. The presence of DARPP-32 (dopamine- and cAMP-regulated phosphoprotein, Mr 32,000) immunoreactivity in COS-1 cells is shown by Western blotting and is consistent with the endogenous expression of a dopamine D1 receptor in these cells. It is concluded that a dopamine D1 receptor is expressed in COS-1 cells and the implications of this are discussed.
authors.affiliation: Abbott Laboratories, Neuroscience Research Division, Pharmaceutical Discovery, Abbott Park, IL 60064.
authors.forename: M E
authors.initials: ME
authors.lastname: Steffey
authors.forename: G L
authors.initials: GL
authors.lastname: Snyder
authors.forename: R W
authors.initials: RW
authors.lastname: Barrett
authors.forename: J S
authors.initials: JS
authors.lastname: Fink
authors.forename: M
authors.initials: M
authors.lastname: Ackerman
authors.forename: P
authors.initials: P
authors.lastname: Adams
authors.forename: R
authors.initials: R
authors.lastname: Bhatt
authors.forename: E
authors.initials: E
authors.lastname: Gomez
authors.forename: R G
authors.initials: RG
authors.lastname: MacKenzie
mesh_terms: D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / Q000494:pharmacology; D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D004298:Dopamine / Q000494:pharmacology; D006054:Goldfish; D007545:Isoproterenol / Q000494:pharmacology; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D011954:Receptors, Dopamine / Q000187:drug effects / Q000502:physiology*; D012160:Retina / Q000378:metabolism; D013268:Stimulation, Chemical
chemical_list: D001552:Benzazepines; D011943:Receptors, Adrenergic, beta; D011954:Receptors, Dopamine; D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine; D000242:Cyclic AMP; D007545:Isoproterenol; D004298:Dopamine