Transcription from a TATA-less promoter requires a multisubunit TFIID complex.
In eukaryotes, the TATA box-binding protein (TBP) is responsible for nucleating assembly of the transcription initiation machinery. Here, we report that a TFIID complex containing TBP is essential for transcription even at a promoter that lacks a TATA box. Immunopurification of TFIID reveals that the active species in reconstituting TATA-less transcription is a multisubunit complex consisting of TBP and many TBP-associated factors (TAFs).
Pugh
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Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Tjian
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D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D004330:Drosophila / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics; D016329:Sp1 Transcription Factor / Q000235:genetics; D016385:TATA Box / Q000235:genetics*; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*
D046911:Macromolecular Substances; D011994:Recombinant Proteins; D016329:Sp1 Transcription Factor; D035362:Transcription Factor TFIID; D014157:Transcription Factors