[Influence of the culture medium (Isosensitest or Mueller-Hinton) on the results of antibiotics susceptibility testing and its interpretation].
The individual results of the antibiotic susceptibility tests of 110 Enterobacteriaceae to 31 antibiotics (beta-lactamines, aminoglycosides, quinolones) have been compared on Mueller-Hinton and Isosensitest. No difference between the diameters of inhibition has been shown by the paired t test or signed rank test. The identification of the phenotypes of resistance (resistance patterns) were compared by principal component analysis and by help of an expert system. The patterns are identical in 99 cases and different in 11 cases. Six pattern were erroneous on Mueller-Hinton and 5 on Isosensitest. Globally both media gave a similar comparable answer in these conditions.
Flandrois
J P
JP
Laboratoire de Biométrie et Dynamique des Populations, Faculté de Médecine Lyon-Sud, URA CNRS 243, Université Lyon I.
Peyret
M
M
Zindel
J
J
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000187:drug effects*; D066298:In Vitro Techniques; D007769:Lactams; D008826:Microbial Sensitivity Tests; D015363:Quinolones / Q000494:pharmacology*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D003470:Culture Media; D007769:Lactams; D015363:Quinolones
Influence du milieu de culture (isosensitest ou Mueller-Hinton) sur le résultat de l'antibiogramme et son interprétation.