Detection of human papillomavirus in cervical swabs from Indian women by cytological and immunocytochemical technique.
Cervical swabs were collected from 88 women in the age group of 17-55 years in Calcutta, India to study the prevalence of human papillomavirus (HPV) infection. The viral infection was demonstrated on the basis of koilocytotic changes in Papanicolaou staining (PAP test) and the presence of viral capsid antigen as detected by immunocytochemistry (IMC test). The PAP and IMC tests revealed 25% and 28.4% of women, respectively, to be positive for HPV infection. Higher frequency (61.1% to 87.5%) of infection was observed in moderate to severe dysplastic lesions. HPV infection was observed to be more frequent in the younger age group (less than 40 years) with 1-2 parity. Although koilocytes were absent in all (n = 51) of the apparently normal subjects, 4 (7.8%) of them were positive for HPV antigen. Except for these, sensitivity of PAP test and IMC test for the detection of HPV was found to be almost similar.
Basu
S
S
Department of Tumor Virology, Chittaranjan National Cancer Institute, Calcutta, India.
Mitra
P K
PK
Roy
A
A
Chatterjee
R
R
D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D002213:Capsid / Q000032:analysis; D002584:Cervix Uteri / Q000382:microbiology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007194:India / Q000453:epidemiology; D008875:Middle Aged; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000276:immunology / Q000302:isolation & purification*; D014412:Tumor Virus Infections / Q000453:epidemiology; D014626:Vaginal Smears
D000956:Antigens, Viral