title: [The detection of rotavirus RNA in nasopharyngeal smears by molecular hybridization].
abstract: Rotavirus RNA was detected in nasopharyngeal washings, obtained from patients with rotavirus gastroenteritis (76.3% of cases) and gastroenteritis of unknown etiology (34.7% of cases), by the method of molecular hybridization with the use of RNA of monkey virus SA11, labeled with 32P in the process of T4-ligase reaction. In 100% of cases this detection correlated with the presence of lesions of the upper respiratory ways and was probably indicative of the rotavirus nature of the catarrhal syndrome in rotavirus gastroenteritis.
authors.forename: N A
authors.initials: NA
authors.lastname: Novikova
authors.forename: E E
authors.initials: EE
authors.lastname: Al'tova
authors.forename: N V
authors.initials: NV
authors.lastname: Noskova
authors.forename: N V
authors.initials: NV
authors.lastname: Epifanova
authors.forename: N A
authors.initials: NA
authors.lastname: Tamoĭkina
mesh_terms: D000208:Acute Disease; D002648:Child; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000382:microbiology*; D009693:Nucleic Acid Hybridization / Q000235:genetics; D015347:RNA Probes; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D012141:Respiratory Tract Infections / Q000382:microbiology; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D015347:RNA Probes; D012367:RNA, Viral
vernacular_title: Obnaruzhenie rotavirusnoĭ RNK v nosoglotochnykh smyvakh metodom molekuliarnoĭ gibridizatsii.