[Studies on human porin. V. The expression of "porin31HL" in the plasmalemma is not by cell transformation].
In recent papers we proved "Porin 31HL" to be located on the surface of human, EBV-transformed B lymphocytes. Here we present proof of "Porin 31HL" in the plasmalemma of normal human blood lymphocytes. For this purpose B and T lymphocytes were isolated from human heparinized blood and examined by indirect immunofluorescence techniques using different monoclonal antibodies against purified "Porin 31HL" and some B and T cell markers, respectively. For comparison a number of established cell lines of different origin were employed. Hence it followed that normal B and T cells as well as transformed and leukemic cells express "Porin 31HL" in their membrane. No significant quantitative differences could be seen. Consequently, the location of "Porin 31HL" in the plasmalemma is not a product of transformation.
König
U
U
Max-Planck-Institut für experimentelle Medizin, Abteilung Immunchemie, Göttingen.
Götz
H
H
Walter
G
G
Babel
D
D
Hohmeier
H E
HE
Thinnes
F P
FP
Hilschmann
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000378:metabolism / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000097:blood*; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology; D002472:Cell Transformation, Viral / Q000502:physiology*; D006224:Cricetinae; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human; D006801:Humans; D008928:Mitochondria / Q000502:physiology; D018272:Porins; D013601:T-Lymphocytes / Q000378:metabolism / Q000502:physiology*
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D018272:Porins
Zur Kenntnis der Porine des Menschen. V. Die Plasmalemmständigkeit von "Porin 31HL" ist keine Folge einer Zell-Transformation.