A new undecasaccharide subunit of xyloglucans with two alpha-L-fucosyl residues.
A new oligosaccharide subunit of xyloglucan was isolated from the beta-(1----4)-endoglucanase digestion products of the xyloglucan in what is referred to as "sycamore extracellular polysaccharides" and found to be an undecasaccharide having two terminal alpha-L-fucopyranosyl residues. The undecasaccharide was structurally characterized by 1H-n.m.r. spectroscopy, fast-atom bombardment mass spectrometry (f.a.b.-m.s.), and glycosyl-residue and glycosyl-linkage composition analyses. The structure of the undecasaccharide was confirmed by digesting it with a hydrolase that releases alpha-D-Xylp-(1----6)-D-Glc from the non-reducing end of xyloglucan oligosaccharides.
Hisamatsu
M
M
Complex Carbohydrate Research Center, University of Georgia, Athens 30602.
Impallomeni
G
G
York
W S
WS
Albersheim
P
P
Darvill
A G
AG
D000215:Acylation; D002240:Carbohydrate Sequence; D002480:Cellulase / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004800:Enzymes, Immobilized; D005643:Fucose / Q000737:chemistry*; D005936:Glucans; D006026:Glycoside Hydrolases; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D011522:Protons; D016339:Spectrometry, Mass, Fast Atom Bombardment; D009626:Terminology as Topic; D014990:Xylans
D004800:Enzymes, Immobilized; D005936:Glucans; D009844:Oligosaccharides; D011134:Polysaccharides; D011522:Protons; D014990:Xylans; D005643:Fucose; C029353:xyloglucan; D006026:Glycoside Hydrolases; C046206:oligoxyloglucan hydrolase; D002480:Cellulase