Characterization and identification of a porcine small intestine mucus receptor for the K88ab fimbrial adhesin.
The ability of Escherichia coli K-12(K88ab) to adhere to immobilized porcine small intestine mucus was examined. E. coli K-12(K88ab) but not the isogenic E. coli K-12 strain was found to adhere readily to immobilized crude mucus but not to bovine serum albumin. The adhesion of E. coli K-12(K88ab) was inhibited in a specific fashion by anti-K88 antiserum. Adhesion was also inhibited by pretreatment of receptor-containing crude mucus preparations with sodium metaperiodate or proteolytic enzymes. Removal of glycolipids from crude mucus by chloroform-methanol extraction did not affect the ability of E. coli K-12(K88ab) to bind to mucus preparations. Adsorption of crude mucus preparations with K88ab fimbriae but not type 1 fimbriae resulted in the removal of K88-specific receptors. Analysis of the pelleted fimbriae-receptor complex by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, together with gel filtration chromatography of crude mucus preparations, suggest that the K88-specific receptor present in porcine small intestine mucus is a 40- to 42-kDa glycoprotein.
Metcalfe
J W
JW
Department of Microbiology, University of Rhode Island, Kingston 02881.
Krogfelt
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KA
Krivan
H C
HC
Cohen
P S
PS
Laux
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DC
D018830:Adhesins, Escherichia coli; D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis*; D002850:Chromatography, Gel; D010450:Endopeptidases / Q000494:pharmacology; D004926:Escherichia coli; D006017:Glycolipids / Q000378:metabolism; D007413:Intestinal Mucosa / Q000737:chemistry / Q000382:microbiology; D007421:Intestine, Small / Q000737:chemistry* / Q000382:microbiology; D010504:Periodic Acid / Q000494:pharmacology; D011817:Rabbits; D011971:Receptors, Immunologic / Q000032:analysis*; D013552:Swine
D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D006017:Glycolipids; D011971:Receptors, Immunologic; C047105:bacterial adhesin receptor; D010504:Periodic Acid; C009288:metaperiodate; D010450:Endopeptidases