[Detection and characterization of antigen component of circulating immune complex in sera from patients with lung cancer by monoclonal and polyclonal antibodies].
A total of 117 serum samples from 58 patients with lung cancer, 29 patients with nonmalignant thoracic diseases and 30 healthy donors were tested and analyzed for antigen component in CIC with 5 McAbs and PcAb by Sandwich-ELISA. The results indicated that McAbs were reactive with lung cancer associated CIC (positive rates ranging from 20-32%), while PcAbs were reactive with CIC both from lung cancer patients and from those with non-malignant thoracic diseases (positive rates 45.7% and 41.4% respectively). A combined set using a panel of McAbs to detect CIC will give a higher positive rate (49.2%) than using a single McAb. We anticipate that the McAb-ELISA established in this study to detect antigen-specific CIC will be helpful in the clinical diagnosis and prognosis of lung cancer.
Xue
W
W
Institute of Basic Medical Sciences, Beijing.
Hou
J
J
D000230:Adenocarcinoma / Q000276:immunology; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000097:blood*; D018288:Carcinoma, Small Cell / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex