title: Transmitter regulation of voltage-dependent K+ channels expressed in Xenopus oocytes.
abstract: Voltage-dependent K+ channels (RBK1, RBK2 and RGK5) were co-expressed in Xenopus oocytes with 5-hydroxytryptamine (5-HT2) receptors. K+ currents measured 2-4 days later were inhibited by 5-HT (100 nM-10 microM, 20-30 s application) by up to 90%. The effect of 5-HT was mimicked by intracellular injection of Ins(1,4,5)P3. Increasing the Ca2+ concentration at the inner surface of excised membrane patches did not decrease the K+ current.
authors.affiliation: Vollum Institute, Oregon Health Sciences University, Portland 97201.
authors.forename: M P
authors.initials: MP
authors.lastname: Kavanaugh
authors.forename: M J
authors.initials: MJ
authors.lastname: Christie
authors.forename: P B
authors.initials: PB
authors.lastname: Osborne
authors.forename: A E
authors.initials: AE
authors.lastname: Busch
authors.forename: K Z
authors.initials: KZ
authors.lastname: Shen
authors.forename: Y N
authors.initials: YN
authors.lastname: Wu
authors.forename: P H
authors.initials: PH
authors.lastname: Seeburg
authors.forename: J P
authors.initials: JP
authors.lastname: Adelman
authors.forename: R A
authors.initials: RA
authors.lastname: North
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology; D003001:Cloning, Molecular; D004553:Electric Conductivity; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology; D008564:Membrane Potentials; D015288:Neurokinin A / Q000494:pharmacology; D009865:Oocytes; D011188:Potassium / Q000502:physiology; D015221:Potassium Channels / Q000502:physiology*; D051381:Rats; D012701:Serotonin / Q000494:pharmacology; D014982:Xenopus laevis
chemical_list: D015221:Potassium Channels; D012701:Serotonin; D015544:Inositol 1,4,5-Trisphosphate; D015288:Neurokinin A; D011188:Potassium; D002118:Calcium