Multiple ligand applications in high-performance immunoaffinity chromatography.
A mixture of antibodies specific for albumin and transferrin, immobilized onto a single support, was used for the simultaneous extraction of albumin and transferrin from immunoglobulin G by high-performance immunoaffinity chromatography. The affinity column was coupled to a strong cation exchanger in order to monitor the success of the extraction and to demonstrate the compatibility of the two chromatographic modes. Coupling of non-affinity chromatography with multiple ligand affinity chromatography is discussed as an alternative to positive affinity chromatography for protein purification.
Wheatley
J B
JB
Rainin Instrument Company, Berkeley, CA 94710.
D002846:Chromatography, Affinity / Q000379:methods*; D002852:Chromatography, Ion Exchange; D006801:Humans; D007106:Immune Sera / Q000737:chemistry; D007074:Immunoglobulin G / Q000302:isolation & purification*; D008024:Ligands; D012709:Serum Albumin / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D014168:Transferrin / Q000302:isolation & purification*
D007106:Immune Sera; D007074:Immunoglobulin G; D008024:Ligands; D012709:Serum Albumin; D014168:Transferrin