title: [The typing of tick-borne encephalitis virus strains by the soluble antigen].
abstract: Sucrose-acetone-treated antigens (SAA) and soluble antigens (SA) prepared from them by treatment with 8 M urea for eight TBE virus strains isolated in different parts of the virus distribution area were studied in cross CFT with immune ascitic fluids (IAF) for these strains as well as for OHF and Powassan viruses. With SAA, the difference in titres with homologous and heterologous IAFs, as a rule, did not exceed twofold, whereas with SA was 32-fold. It was also noted that in the homologous system the titre of SA decreased not more than 4-fold as compared with that of the initial SAA while in the heterologous system it declined from 4- to 16-fold or more. The level of antigenic relationship of strains was expressed by the formula HAg = SA titre/SA titre X 100%. It was shown that at HAg greater than or equal to 25% the strains used for antigen and antibody generation were closely related and at HAg less than or equal to 6.25% belonged to different subtypes. The analysis of the data suggested the existence of a fourth, "Urals-Siberian" antigenic variant of TBE virus. Using the developed criteria, the 20 strains under study (with one exception) were alloted into one of the four antigenic variants or qualified as intermediate.
authors.forename: V I
authors.initials: VI
authors.lastname: Zlobin
authors.forename: D A
authors.initials: DA
authors.lastname: Drokin
authors.forename: P G
authors.initials: PG
authors.lastname: Mansurov
authors.forename: O B
authors.initials: OB
authors.lastname: Kalmin
authors.forename: V V
authors.initials: VV
authors.lastname: Iakimenko
mesh_terms: D000818:Animals; D000940:Antigenic Variation / Q000276:immunology; D000956:Antigens, Viral / Q000145:classification* / Q000276:immunology; D001103:Arboviruses / Q000145:classification / Q000276:immunology; D001202:Ascitic Fluid / Q000276:immunology; D004669:Encephalitis Viruses, Tick-Borne / Q000145:classification* / Q000276:immunology; D006481:Hemorrhagic Fever, Omsk / Q000382:microbiology; D007114:Immunization; D051379:Mice; D012703:Serotyping / Q000379:methods; D012995:Solubility; D013045:Species Specificity
chemical_list: D000956:Antigens, Viral
vernacular_title: Tipirovanie shtammov virusa kleshchevogo éntsefalita po rastvorimomu antigenu.