[A study on the male DNA special restricted fragment of bovine].
Bkm, which is related to sexes, is a satellite DNA of the female snake. After the genomic DNA of bovine was digested by Pst I, it was hybridized with Bkm sequence probe (p184) and subcloned in the vector pUC19. Recombinants were identified by the colony in situ and Southern blot hybridization. When we determined genomic DNA of bovine using the male DNA fragment, it was observed that the pattern of hybridization was different because of different sexes. The results showed that we could identify the sex of bovine by Southern blot hybridization with recombinants of male DNA special restricted fragment of bovine origin.
Li
M
M
Department of Biochemistry.
Sun
Z
Z
Shi
Y
Y
D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004276:DNA, Satellite; D005260:Female; D005819:Genetic Markers; D008297:Male; D009693:Nucleic Acid Hybridization; D012732:Sex Determination Analysis; D012911:Snakes
D015342:DNA Probes; D004274:DNA, Recombinant; D004276:DNA, Satellite; D005819:Genetic Markers; D004262:DNA Restriction Enzymes