Fitness alteration of foot-and-mouth disease virus mutants: measurement of adaptability of viral quasispecies.
We document the rapid alteration of fitness of two foot-and-mouth disease virus (FMDV) mutants resistant to a neutralizing monoclonal antibody. Both mutants showed a selective disadvantage in BHK-21 cells when passaged in competition with their parental FMDV. Upon repeated replication of the mutants alone, they acquired a selective advantage over the parental FMDV and fixed additional genomic substitutions without reversion of the monoclonal antibody-resistant phenotype. Thus, variants that were previously kept at low frequency in the mutant spectrum of a viral quasispecies rapidly became the master sequence of a new genomic distribution and dominated the viral population.
Martínez
M A
MA
Departamento de Sanidad Animal, Instituto Nacional de Investigaciones Agrarias, Madrid, Spain.
Carrillo
C
C
González-Candelas
F
F
Moya
A
A
Domingo
E
E
Sobrino
F
F
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005537:Aphthovirus / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D005075:Biological Evolution; D002460:Cell Line; D006224:Cricetinae; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009154:Mutation; D009500:Neutralization Tests; D013045:Species Specificity; D014779:Virus Replication
D000911:Antibodies, Monoclonal