A soluble form of the human T cell differentiation antigen CD27 is released after triggering of the TCR/CD3 complex.
The human T cell Ag CD27 belongs to a recently defined family of cell surface receptors, including the nerve growth factor receptor, two distinct tumor necrosis factor receptors, and the B cell specific molecule CD40. On resting T cells, CD27 is a transmembrane homodimer with subunits of 50 to 55 kDa (p55). T cell activation via the TCR/CD3 complex causes a strong enhancement of p55 expression. Concomitantly, an alternative form of the CD27 molecule with a molecular mass of 28 to 32 kDa (p32) appears at the cell surface. With the use of ELISA, we here show that a soluble form of CD27 (sCD27) can be detected in the supernatant of T cells activated with anti-CD3 or combinations of anti-CD2 mAb. Moreover, sCD27 is found in both serum and urine from healthy donors. sCD27, purified from either culture supernatant or urine, has a molecular mass of 28 to 32 kDa and is, according to peptide mapping, structurally homologous to the p55 membrane form of CD27. Quantification of sCD27 levels may be used as a marker for T lymphocyte activation in vivo.
Hintzen
R Q
RQ
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
de Jong
R
R
Hack
C E
CE
Chamuleau
M
M
de Vries
E F
EF
ten Berge
I J
IJ
Borst
J
J
van Lier
R A
RA
D015703:Antigens, CD / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D017252:CD3 Complex; D002462:Cell Membrane / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism; D010449:Peptide Mapping; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D015398:Signal Transduction; D012995:Solubility; D013601:T-Lymphocytes / Q000502:physiology*; D013997:Time Factors; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7