title: Brown adipose tissue mitochondria are cytochrome c-subsaturated.
abstract: The oxidative activity of mitochondria freshly isolated from brown adipose tissue of rats was stimulated two-fold on the addition of small concentrations of exogenous cytochrome c to the reaction medium. Loss of membrane-bound cytochrome c did not occur during isolation of mitochondria. Estimation of the high-affinity binding sites on the organelle membrane indicated that less than a third of these sites remained saturated with cytochrome c. The pigment is thus shown to be a functionally limiting electron transport component in brown adipose tissue.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore.
authors.forename: A S
authors.initials: AS
authors.lastname: Gaikwad
authors.forename: T
authors.initials: T
authors.lastname: Ramasarma
authors.forename: C K
authors.initials: CK
authors.lastname: Ramakrishna Kurup
mesh_terms: D002001:Adipose Tissue, Brown / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002458:Cell Fractionation; D002952:Citric Acid Cycle; D003574:Cytochrome c Group / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D005994:Glycerophosphates / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000737:chemistry; D010101:Oxygen Consumption / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003574:Cytochrome c Group; D005994:Glycerophosphates; C029620:alpha-glycerophosphoric acid