Cloning of a cDNA encoding an Artemia franciscana Na/K ATPase alpha-subunit.
Clones of cDNA that code for an isoform of the Artemia franciscana Na/K ATPase alpha subunit (NaKA alpha) have been isolated. The sequence of the longest of these clones (pArATNa136) is 3595 nucleotides; it codes for a 1004-amino acid protein whose sequence is identical to that of two previously sequenced Artemia NaKA alpha peptides. The encoded protein is over 73% identical to Drosophila melanogaster and vertebrate NaKA alpha s, and 73.8% identical to another Artemia NaKA alpha isoform previously described (named alpha 2850 in this article). The two Artemia cDNA clones code for mRNAs of different size; the clone pArATNa136 codes for a 4.5-kb mRNA while the alpha 2850 clone codes for a 3.6-kb mRNA. The degree of homology and the different size of the mRNAs encoded by both cDNAs suggest that they code for two different isoforms of the protein.
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Instituto de Investigaciones Biomédicas del C.S.I.C., Madrid, Spain.
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D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D015183:Restriction Mapping; D016415:Sequence Alignment; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000378:metabolism
D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D000254:Sodium-Potassium-Exchanging ATPase