Related RNA polymerase-binding regions in human RAP30/74 and Escherichia coli sigma 70.
RAP30/74 is a heteromeric general transcription initiation factor that binds to mammalian RNA polymerase II. The RAP30 subunit contains a region that is similar in amino acid sequence to the RNA polymerase-binding domain of the Escherichia coli transcription initiation factor sigma 70 (sigma 70). Mammalian RNA polymerase II specifically protected a serine residue in the sigma 70-related region of RAP30 from phosphorylation in vitro. In addition, human RAP30/74 bound to Escherichia coli RNA polymerase and was displaced by sigma 70. These results suggest that RAP30 and sigma 70 have functionally related RNA polymerase-binding regions.
McCracken
S
S
Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.
Greenblatt
J
J
D000595:Amino Acid Sequence; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D003488:Cyanogen Bromide; D000242:Cyclic AMP / Q000494:pharmacology; D004926:Escherichia coli / Q000032:analysis* / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism*; D012808:Sigma Factor / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D019473:Transcription Factors, TFII; D014357:Trypsin
D010446:Peptide Fragments; D012808:Sigma Factor; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D000242:Cyclic AMP; D011494:Protein Kinases; D012319:RNA Polymerase II; D014357:Trypsin; C056604:transcription factor TFIIF; D003488:Cyanogen Bromide