Continuous complete collection of uncontaminated urine in conscious rodents.
We describe a novel technique for complete and continuous bladder urine collection in conscious unrestrained rats. After urethral ligation, a silastic catheter is placed through a flexible steel tether, tunneled subcutaneously from the posterior neck area to a suprapubic incision, and inserted and fixed into the exposed urinary bladder. The catheter drains by gravity into a dependent collecting vessel protected from contamination by feces or food.
Mandavilli
U
U
Department of Surgery, Massachusetts General Hospital, Boston.
Schmidt
J
J
Rattner
D W
DW
Watson
W T
WT
Warshaw
A L
AL
D000818:Animals; D005243:Feces; D005502:Food; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012149:Restraint, Physical; D013048:Specimen Handling / Q000379:methods / Q000592:standards; D001743:Urinary Bladder; D014546:Urinary Catheterization / Q000295:instrumentation / Q000379:methods* / Q000662:veterinary; D014556:Urine