Isolation and molecular evolutionary analysis of a cytochrome c gene from Oryza sativa (rice)
A cytochrome c gene, OsCc-1, from rice (Oryza sativa) has been isolated and analyzed. The OsCc-1 gene encodes a cytochrome c protein that is typical of higher-plant cytochrome c proteins. OsCc-1 consists of three exons separated by two introns that are 817 and 747 bp in length, respectively. From genomic DNA hybridization analysis, OsCc-1 appears to be one of possibly two cytochrome c genes in several Asian, American, and Indian rice species and varieties surveyed. A single, unique cytochrome c gene appears to be present in one African cultivated rice species. We performed comparative molecular evolutionary analyses of OsCc-1 and other cytochrome c genes. We calculated a unit evolutionary period of 19.4 Myr for cytochrome c DNA sequences, which agrees closely with previous estimates based on protein sequence comparisons.
Kemmerer
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Field of Botany, Cornell University, Ithaca, New York 14853.
Lei
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Wu
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D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D003574:Cytochrome c Group / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D003574:Cytochrome c Group