[In vitro study of the cytotoxicity of inflammation mediators secreted around microencapsulated cells].
One of the implantation problems in immunoprotected living cells is the appearance of local inflammatory phenomena around microcapsules. Some of the mediators released in such pathophysiological conditions were tested. A toxic action of compounds such as elastase, collagenase was evidenced. Interleukins 1 and 2 revealed no cytotoxicity within the test limits on the experimental cellular model chosen. These results underline the importance of inflammatory mediators released by adjacent cells of the implant.
Vitiello
S
S
INSERM U306, Université de Bordeaux II.
Cadic
C
C
Dupuy
B
B
D000818:Animals; D002214:Capsules / Q000009:adverse effects*; D002460:Cell Line / Q000637:transplantation; D003602:Cytotoxicity, Immunologic; D066298:In Vitro Techniques; D007249:Inflammation / Q000201:enzymology / Q000276:immunology*; D003012:Microbial Collagenase / Q000378:metabolism / Q000633:toxicity; D010196:Pancreatic Elastase / Q000378:metabolism / Q000633:toxicity; D051381:Rats
D002214:Capsules; D010196:Pancreatic Elastase; D003012:Microbial Collagenase
Etude in vitro de la cytotoxicité de médiateurs de l'inflammation sécrétés autour de cellules microencapsulées.