In vitro production of free oxygen radicals induced by pulsed ultrasounds in whole blood exposed to diagnostical frequencies and intensities.
DNA alkalinization experiments on lymphocytes from sonicated whole blood and on in vitro cultured lymphocytes in presence of free radical scavengers (superoxide dismutase, catalase and mannitol) showed that lesions inflicted upon DNA by pulsed ultrasounds could be ascribed to production of free radicals (O2-, OH.) and H2O2, which could mediate the production of still unidentified organic radicals, likely to be responsible for DNA damage.
Chicca
M
M
Department of Evolutive Biology, University of Ferrara.
Muzzoli
M
M
Pinamonti
S
S
D001769:Blood; D002478:Cells, Cultured; D003574:Cytochrome c Group / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D016166:Free Radical Scavengers; D005609:Free Radicals; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D013010:Sonication / Q000009:adverse effects*; D013481:Superoxides / Q000378:metabolism
D003574:Cytochrome c Group; D016166:Free Radical Scavengers; D005609:Free Radicals; D013481:Superoxides; D010100:Oxygen