Localization of 616 human chromosome 3-specific cosmids using a somatic cell hybrid deletion mapping panel.
A total of 5700 human chromosome 3-specific cosmid clones was isolated from a series of cosmid libraries constructed from somatic cell hybrids whose only human component was an entire chromosome 3 or a chromosome 3 containing an interstitial deletion removing 50% of long arm sequences. Several unique sequence chromosome 3-specific hybridization probes were isolated from each of 616 of these cosmids. These probes were then used to localize the cosmids by hybridization to a somatic cell hybrid deletion mapping panel capable of resolving chromosome 3 into nine distinct subregions. All 616 of the cosmids were localized to either the long or short arm of chromosome 3 and 63% of the short arm cosmids were more precisely localized. We have identified a total of 87 cosmids that contain fragments that are evolutionarily conserved. Fragments from these cosmids should prove useful in the identification of new chromosome 3-specific genes as well as in comparative mapping studies. The localized cosmids should provide excellent saturation of human chromosome 3 and facilitate the construction of physical and genetic linkage maps to identify various disease loci including Von Hippel Lindau disease and renal and small cell lung carcinoma.
Smith
D I
DI
Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, Michigan 48201.
Liu
W
W
Ginzinger
D
D
Green
P
P
Smith
S
S
Wang
N D
ND
Recchia
F
F
Carolyn
K
K
Drabkin
H
H
Golembieski
W
W
D000818:Animals; D016466:CHO Cells; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D003360:Cosmids; D006224:Cricetinae; D015342:DNA Probes; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015698:Genomic Library; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic
D015342:DNA Probes; D004262:DNA Restriction Enzymes