title: Absence of mutagenic activity in Salmonella and of clastogenic activity in CHO cells of Caramel Colours I, II, III and IV.
abstract: A total of 15 caramel colours were examined for genotoxic activity using the Salmonella typhimurium plate incorporation assay (Ames test). Five bacterial strains, TA1535, TA1537, TA1538, TA98 and TA100 were used in all the plate incorporation tests. Caramel colours can be divided into four classes, classification depending on the preparative method used. In this study, representatives of all four classes of caramel colour were tested for genotoxic potential in the Ames test, some of the caramel colours being tested both with and without a pre-incubation stage. None of the 15 caramel colours tested exhibited genotoxic potential in any of the five bacterial tester strains. The last two caramel colours tested, in the series of 15 [203-23-4 (Class II) and 311 (Class III)] were also assessed for clastogenic potential. For this test, cultures of CHO cells were exposed to the two caramel colours and metaphase preparations from these cultures examined for evidence of chromosomal aberrations. No evidence of chromosome damaging activity was observed.
authors.affiliation: Department of Mutagenesis and Cell Biology, Huntingdon Research Centre.
authors.forename: J A
authors.initials: JA
authors.lastname: Allen
authors.forename: P C
authors.initials: PC
authors.lastname: Brooker
authors.forename: E
authors.initials: E
authors.lastname: Jones
authors.forename: K
authors.initials: K
authors.lastname: Adams
authors.forename: M
authors.initials: M
authors.lastname: Richold
mesh_terms: D000818:Animals; D016466:CHO Cells; D002182:Candy; D002241:Carbohydrates; D002875:Chromosomes / Q000187:drug effects*; D006224:Cricetinae; D005505:Food Coloring Agents / Q000633:toxicity*; D005796:Genes / Q000187:drug effects*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009930:Organic Chemicals; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
chemical_list: D002241:Carbohydrates; D005505:Food Coloring Agents; D009153:Mutagens; D009930:Organic Chemicals; C569050:caramel coloring