title: Simple methods for making EcoK and McrA restrictionless mutants.
authors.affiliation: Genetics Division, National Institute for Medical Research, Mill Hill, London, UK.
authors.forename: K
authors.initials: K
authors.lastname: Hiom
authors.forename: S G
authors.initials: SG
authors.lastname: Sedgwick
mesh_terms: D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015253:Deoxyribonucleases, Type I Site-Specific / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D009154:Mutation; D013014:SOS Response, Genetics; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays
chemical_list: D004262:DNA Restriction Enzymes; C029602:endodeoxyribonuclease EcoK; C060629:endodeoxyribonuclease McrA; D015253:Deoxyribonucleases, Type I Site-Specific