title: Sodium pump activity and contraction of renal artery from spontaneously hypertensive rats.
abstract: We investigated the hypertensive changes in renal arteries isolated from 21-week-old spontaneously hypertensive rats (SHR), and from age-matched normotensive Wistar-Kyoto rats (WKY). The maximum contraction of renal arteries from SHR in response to norepinephrine (NE), serotonin (5-HT) and KCl was greater than that of arteries from WKY. The threshold and EC50 concentrations of NE, 5-HT and KCl were not significantly different between SHR and WKY. Contraction induced by removal of K+ was inhibited by 10(-8) M prazosin. Less than 10(-7) M NE in K(+)-free solution did not cause contraction. Addition of 5.9 mM KCl to K(+)-free solution in the presence of 10(-5) M NE induced relaxation, which was followed by contraction to about the same level as that before KCl addition. The duration of the K(+)-induced relaxation in SHR (22.4 +/- 0.9 min) was slightly, but significantly shorter than that in WKY (26.6 +/- 0.8 min) arteries. In K(+)-free solution with reduced Na+, the duration of the relaxation induced by KCl was shorter than that in the normal solution, for both SHR (13.8 +/- 0.3 min) and WKY (14.1 +/- 0.5 min). Such differences could be caused by increased influx and decreased efflux of Ca2+, which depend on the Na+ concentration and are related to the Na(+)-Ca2+ exchange. The results suggest that enhanced renal vascular reactivity in hypertension may depend on structural changes and increased Na+ pump activity.
authors.affiliation: 2nd Department of Physiology, Showa University, School of Medicine, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakai
authors.forename: M
authors.initials: M
authors.lastname: Inazu
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D006973:Hypertension / Q000378:metabolism; D066298:In Vitro Techniques; D007668:Kidney / Q000033:anatomy & histology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects* / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology; D009929:Organ Size / Q000502:physiology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012077:Renal Artery / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000502:physiology*
chemical_list: D012701:Serotonin; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium; D009638:Norepinephrine