[Comparison of immunofluorescence reagents for the detection of the early antigen of cytomegaloviruses in the Shell-vial].
Detection of cytomegalovirus has been carried out in 178 clinical samples in order to compare the effectiveness of two distinct commercial immunofluorescence reagents: anti-CMV early nuclear protein (Du Pont) and CMV culture identification (Syva Micro-Trak). In 61 of the samples we observed positive reaction to either of the two procedures (Shell-vial and, or, cellular conventional culture). Sensitivity was 0.92 for Du Pont reagent, 0.79 for Syva reagent, and 0.93 for the conventional cellular culture. In the overall samples we observed a higher incidence of fluorescent focus using Du Pont reagent that using that of Syva.
Mendoza Montero
J
J
Servicio de Microbiología, Ciudad Sanitaria Virgen de las Nieves, Granada.
Navarro Marí
J M
JM
Rojas González
A
A
Plata Rosales
J C
JC
de la Rosa Fraile
M
M
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006801:Humans; D017874:Immediate-Early Proteins; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D014776:Virus Cultivation
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D011933:Reagent Kits, Diagnostic; C044582:immediate-early proteins, cytomegalovirus
Comparación de reactivos de inmunofluorescencia para la detección de antígeno precoz de citomegalovirus en Shell-vial.