Potency assessment of foot-and-mouth disease vaccines in cattle by means of antibody assays.
A tendency has emerged for some years to replace the challenge infection of cattle for the assessment of foot-and-mouth disease (FMD) vaccine potency. This can be actually evaluated by means of antibody assays on cattle sera, at about 3/4 weeks after the vaccination. Serological results can be worked out as single titres (to be compared with a pre-determined threshold level) or as mean antibody titres induced by different vaccine dilutions. However, the assessment of FMDV-specific antibody titres would not fully depict the extent and the efficacy of the immune response of cattle; moreover, the antibody response would not be proportional if potent vaccines are used (greater than or equal to 10-12 PD50). Thus, a particular approach is suggested for the serological procedures, which enable credible estimates of potent FMD vaccines to be formulated.
Amadori
M
M
Istituto Zooprofilattico Sperimentale della Lombardia e dell 'Emilia, Brescia, Italy.
Archetti
I L
IL
Tollis
M
M
Buonavoglia
C
C
Panina
G F
GF
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D005537:Aphthovirus / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000517:prevention & control; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000008:administration & dosage / Q000032:analysis / Q000276:immunology*
D000914:Antibodies, Viral; D014765:Viral Vaccines