Gyrase inhibitors increase the content of knotted DNA species of plasmid pBR322 in Escherichia coli.
Treatment of Escherichia coli cells harboring pBR322 with the DNA gyrase inhibitors oxolinic acid and coumermycin A1 led to an increase in the content of knotted pBR322 molecules. This phenomenon was attributed to inhibition of gyrase-catalyzed unknotting of the plasmid DNA knotted by transcription.
Ishii
S
S
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
Murakami
T
T
Shishido
K
K
D049933:Aminocoumarins; D003374:Coumarins / Q000494:pharmacology; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial; D009690:Nucleic Acid Conformation; D010093:Oxolinic Acid / Q000494:pharmacology; D010957:Plasmids; D059005:Topoisomerase II Inhibitors; D014158:Transcription, Genetic
D049933:Aminocoumarins; D003374:Coumarins; D004269:DNA, Bacterial; D059005:Topoisomerase II Inhibitors; D010093:Oxolinic Acid; C004628:coumermycin