Membrane depolarization and the expression of glutamate receptors in cerebellar granule cells.
The functional expression of glutamate receptors in cerebellar granule cells in culture was examined using the whole-cell patch clamp technique. All the cells tested responded with an inward current to superfusion with glutamate, kainate, or alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate. Neurones cultured in a medium containing an elevated K+ concentration (25 mM) responded to N-methyl-D-aspartate (NMDA) provided they were simultaneously exposed to glycine (Gly). In contrast, NMDA/Gly-evoked currents were virtually undetectable in cells grown in medium containing 'low' K+ (10 mM), suggesting that only the NMDA-preferring class of glutamate receptors are subject to up-regulation by K(+)-induced chronic membrane depolarization.
Van der Valk
J B
JB
Research Institute of Toxicology, University of Utrecht, The Netherlands.
Resink
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Balázs
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D000596:Amino Acids / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D004594:Electrophysiology; D008564:Membrane Potentials / Q000187:drug effects; D009467:Neuromuscular Depolarizing Agents / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
D000596:Amino Acids; D009467:Neuromuscular Depolarizing Agents; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D011188:Potassium