Direct measurement of antiviral activities by a novel immunoassay with peroxidase-labeled complement C1q.
A novel immunoassay method for evaluating antiviral activities has been developed. The method involves the specific measurement of viral antigens produced in cells infected with herpes simplex virus (HSV) as a model by using the anti-HSV probe serum to detect HSV antigens and the peroxidase-labeled complement C1q to detect the immune complex formed. A new compound, heteropolyoxotungstate K7[PTi2W10O40]. 6H2O, was proven to have an anti-herpetic activity at the concentration of 5.0 micrograms/ml determined by the novel immunoassay.
Taguchi
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Department of Microbiology, Kitasato University, School of Hygienic Sciences, Kanagawa-ken, Japan.
Kawasaki
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Lee
S M
SM
Yamamoto
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A
D000212:Acyclovir / Q000494:pharmacology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D000998:Antiviral Agents / Q000494:pharmacology*; D015922:Complement C1q / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D011108:Polymers / Q000494:pharmacology; D018139:Simplexvirus / Q000187:drug effects* / Q000276:immunology; D014414:Tungsten / Q000494:pharmacology; D017973:Tungsten Compounds; D014709:Vero Cells
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D000998:Antiviral Agents; D011108:Polymers; D017973:Tungsten Compounds; D015922:Complement C1q; C064098:polyoxotungstate PM 19; D014414:Tungsten; D000212:Acyclovir