Increased superoxide production by polymorphonuclear leukocytes in systemic lupus erythematosus.
Normal and lupus PMN show an enhancement in superoxide production in vitro when stimulated with lupus serum. When N-formyl-methionyl-leucyl-phenylalanine (FMLP) was used, lupus PMN showed an O2- production of 2.1 nmol/min/10(7) cells, which is 5.2 times the response of normal PMN stimulated by FMLP. Our results show the existence of serum factors in SLE patients that can stimulate O2- production by PMN. Lupus neutrophils showed an increased response to membrane stimuli such as FMLP, capable of triggering the cell respiratory burst. Lupus neutrophils appeared more responsive to membrane stimuli. The serum and cellular factors seemed to indicate an increase rate of superoxide production by PMN in lupus patients, which could be relevant factors in the development of vasculitis and tissue damage.
Casellas
A M
AM
Alfredo Lanari Institute of Medical Research, Faculty of Medicine, University of Buenos Aires, Argentina.
Prat
A
A
Llera
A
A
Manni
J
J
Boveris
A
A
Sarano
J F
JF
D002630:Chemotactic Factors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D016897:Respiratory Burst / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism*
D002630:Chemotactic Factors; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine