Estimation of avidin activity by two methods.
The biological activity of avidin was estimated by two different methods. The spectrophotometric method used the avidin titration with biotin in the presence of 4 hydroxiazobenzen-2'carboxilic acid as indicator. In the radioisotopic determination the titration with tritiated biotin was accomplished. Both methods led to the same results, but the spectrophotometric one is less avidin expensive and more rapid, being more convenient.
Borza
B
B
Faculty of Chemistry, University of Bucharest, Romania.
Marcheş
F
F
Repanovici
R
R
Burducea
O
O
Popa
L M
LM
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001360:Avidin / Q000032:analysis* / Q000302:isolation & purification; D001391:Azo Compounds / Q000138:chemical synthesis; D001710:Biotin; D005069:Evaluation Studies as Topic; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D007202:Indicators and Reagents; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
D000914:Antibodies, Viral; D001391:Azo Compounds; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D001360:Avidin; C001413:HABA; D001710:Biotin