Isolation of DNA from blood.
A rapid, economical method of DNA isolation from blood was developed that yields DNA suitable for Southern analysis and polymerase chain reactions without organic solvent extractions. Bovine DNA was prepared from peripheral leukocytes and nuclei using pronase E digestion and ethanol precipitation. This isolation method readily adapts to multiple samples. The DNA is characterized by high yield, solubility, lack of protein contamination, and ease of restriction endonuclease digestion.
Kendall
T L
TL
University of Georgia, Department of Animal and Dairy Science, Athens, Georgia 30602.
Byerley
D J
DJ
Dean
R
R
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000737:chemistry; D011232:Chemical Precipitation; D004247:DNA / Q000097:blood / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D000431:Ethanol; D008297:Male; D011402:Pronase; D013094:Spermatozoa / Q000737:chemistry
D000431:Ethanol; D004247:DNA; D004262:DNA Restriction Enzymes; D011402:Pronase