[Cloning and expression of the 3C-proteinase gene from the poliomyelitis virus in E. coli cells].
Expression of the 3C protease gene of poliovirus type 1 (Mahoney) in E. coli cells using various vectors was studied. The 3C gene was shown to be expressed effectively upon its cloning in HindII/HindII (bases 5240 to 6770) and in HindII/HindIII (bases 5240 to 6056) fragments of poliovirus cDNA in pTTQ8 vector containing tac-promoter and lacI-repressor gene. Products of processing at the N-terminal 3C protease Gln-Gly site and polypeptides formed upon translation from an alternative methionine, which was coded by bases 5516-5518 of poliovirus cDNA, were found among virus-specific proteins. Processing at the C-terminal 3C protease Gln-Gly site was not observed.
Sablina
E P
EP
Amerik
A Iu
AIu
Antonov
V K
VK
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology*; D015870:Gene Expression; D005822:Genetic Vectors; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D017955:Poliovirus / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D011499:Protein Processing, Post-Translational; D015183:Restriction Mapping
D010450:Endopeptidases
Konirovanie i ékspressiia gena 3C-proteinazy virusa poliomielita v kletkakh E. coli.