title: Dialkylphosphorus metabolites in the urine and activities of esterases in the serum as biochemical indices for human absorption of organophosphorus pesticides.
abstract: Ninety-seven agricultural workers were monitored for absorption of the organophosphorus pesticides methidathion, vamidothion, and azinphos-methyl, which were sprayed in an orchard during two seasons. Low levels of only one dialkylphosphorus metabolite (dimethyl phosphorothioate) were found in only eight workers in pre-exposure urine samples. More than one dialkylphosphorus metabolite was detected in almost all exposed individuals in after-exposure urine samples. The highest concentrations were measured after exposure to azinphos-methyl; the median concentrations of dimethyl phosphorodithioate and dimethyl phosphorothioate were 0.92 and 0.78 nmol/mg creatinine with a concentration range up to 14.3 and 53.7, respectively. Three diethylphosphorus metabolites were also detected in some samples, but at lower concentrations. Cholinesterase activities were decreased (31-48%) in the serum of 12 workers; four of those workers had no dialkylphosphorus metabolites in the urine. Paraoxonase and arylesterase activities in the serum were unaffected by the absorption of pesticides, and there was no correlation between the activities of these esterases and the metabolite concentrations in the urine. This study confirmed that dialkylphosphorus metabolites in the urine are a more sensitive index of absorption than cholinesterase inhibition in the serum but lack of correlation between cholinesterase inhibition and metabolite concentration indicates that both parameters should be monitored.
authors.affiliation: Institute for Medical Research and Occupational Health, University of Zagreb, Yugoslavia.
authors.forename: V
authors.initials: V
authors.lastname: Drevenkar
authors.forename: Z
authors.initials: Z
authors.lastname: Radić
authors.forename: Z
authors.initials: Z
authors.lastname: Vasilić
authors.forename: E
authors.initials: E
authors.lastname: Reiner
mesh_terms: D043303:Aryldialkylphosphatase; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000097:blood*; D002800:Cholinesterase Inhibitors / Q000633:toxicity; D002802:Cholinesterases / Q000097:blood*; D002849:Chromatography, Gas; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D016273:Occupational Exposure; D009943:Organophosphorus Compounds / Q000652:urine*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors / Q000097:blood*
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D009943:Organophosphorus Compounds; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D002802:Cholinesterases; D010744:Phosphoric Monoester Hydrolases; D043303:Aryldialkylphosphatase