Splicing of the Drosophila P element ORF2-ORF3 intron is inhibited in a human cell extract.
P element transposition in Drosophila melanogaster is regulated by germline-specific splicing of the P element ORF2-ORF3 intron. This regulation has been shown to depend on a cis-acting sequence located in the exon 12-31 bases from the 5' splice site. Mutations within this sequence disrupt the regulation and result in splicing of the ORF2-ORF3 intron in all tissues, indicating that the sequence is required to inhibit splicing of this intron in the soma. We now show that a trans-acting factor in a human (HeLa) cell extract can inhibit splicing of the intron, suggesting that this regulatory mechanism is conserved from flies to humans.
Tseng
J C
JC
Molecular Biology Institute, University of California, Los Angeles 90024.
Zollman
S
S
Chain
A C
AC
Laski
F A
FA
D000818:Animals; D001483:Base Sequence; D002457:Cell Extracts / Q000494:pharmacology*; D004251:DNA Transposable Elements / Q000235:genetics*; D004330:Drosophila; D005091:Exons / Q000187:drug effects / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007438:Introns / Q000187:drug effects*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010455:Peptides / Q000528:radiation effects; D012326:RNA Splicing / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics; D014466:Ultraviolet Rays
D002457:Cell Extracts; D004251:DNA Transposable Elements; D010455:Peptides; D012333:RNA, Messenger