Effects of protein kinase C inhibitors on viral entry and infectivity.
The protein kinase C inhibitor H-7 (2-20 microM) inhibited dose-dependently the infectivity of the vesicular stomatitis virus on cultured human fibroblasts. Electron microscopy showed that H-7 inhibited the viral entry. H-7 also inhibited the infectivity of four other enveloped viruses, herpes simplex I, turkey herpes, vaccinia and Sindbis. Similar results were obtained using staurosporine (2.5 nM), tamoxifen (40 microM), phloretin (140 microM), or W-7 (40 microM). However, the infectivity of non-enveloped viruses (e.g. poliomyelitis I) was not inhibited by H-7. These results show that protein kinase C is critically involved in the infectivity of enveloped viruses, most probably at the level of viral entry (receptor-mediated endocytosis).
Constantinescu
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Division of Cell Biology, Faculty of Medicine, Bucharest, Romania.
Cernescu
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Popescu
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LM
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004705:Endocytosis; D005347:Fibroblasts / Q000382:microbiology* / Q000648:ultrastructure; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology*; D008854:Microscopy, Electron; D010879:Piperazines / Q000494:pharmacology*; D017955:Poliovirus / Q000187:drug effects / Q000502:physiology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D018139:Simplexvirus / Q000187:drug effects / Q000502:physiology; D012845:Sindbis Virus / Q000187:drug effects / Q000502:physiology; D014616:Vaccinia virus / Q000187:drug effects / Q000502:physiology; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000502:physiology; D018406:Virus Physiological Phenomena; D014780:Viruses / Q000187:drug effects
D007546:Isoquinolines; D010879:Piperazines; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C