Decay of mammalian hexokinase: characterization of the specific proteolytic activity.
Hexokinase-degrading activity (HKDA) is associated with hexokinase throughout purification. It is not ubiquitin-dependent. With glucose present, HKDA requires MgATP. It is triggered by removal (or ATP-dependent consumption) of glucose, and masked when glucose is restored. PCMB, TLCK and TPCK suppress hexokinase and activate HKDA. Glucose removal and active-site blockage appear to induce HKDA.
Murakami
K
K
Division of Pediatric Hematology and Oncology, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Piomelli
S
S
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000201:enzymology*; D005947:Glucose / Q000378:metabolism; D006593:Hexokinase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D011480:Protease Inhibitors / Q000494:pharmacology*; D012156:Reticulocytes / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism
D011480:Protease Inhibitors; D014452:Ubiquitins; D000255:Adenosine Triphosphate; D006593:Hexokinase; D005947:Glucose