Vitamin D receptors in heart: effects on atrial natriuretic factor.
We report that receptors for vitamin D exist in distinct regions of the heart in female and male mice, predominantly in the right atrium where most of the cardial atrial natriuretic peptide (ANF) is produced. Tritiated 1,25-dihydroxyvitamin D3 (1,25-D3, vitamin D, soltriol) and ANF are colocalized in nuclei and cytoplasm respectively in identical cardiomyocytes. Changes of ANF tissue and blood levels under dietary deficiency and treatment with 1,25-D3 suggest direct genomic actions of vitamin D on myoendocrine cells of the atrium for the regulation of ANF manufacture and secretion. These results were obtained by combining thaw-mount autoradiography with immunocytochemistry using tritiated 1,25-D3 and an antibody against rat ANF. This antibody was also used in a radioimmunoassay to determine atrial natriuretic factor in plasma, atria and ventricles of normal or vitamin D-deficient mice.
Bidmon
H J
HJ
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599-7090.
Gutkowska
J
J
Murakami
R
R
Stumpf
W E
WE
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000378:metabolism*; D002117:Calcitriol / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry; D005260:Female; D006325:Heart Atria / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000737:chemistry / Q000502:physiology*
D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D009320:Atrial Natriuretic Factor; D002117:Calcitriol