In vivo and in vitro sodium pump activity in subjects with thyrotoxic periodic paralysis.
OBJECTIVE
To examine whether sodium pump activity plays a part in the pathogenesis of thyrotoxic periodic paralysis.


METHODS
Measurement of platelet sodium-potassium ATPase and in vivo sodium pump activities in healthy subjects and thyrotoxic subjects with and without paralysis.


METHODS
University hospital in Hong Kong.


METHODS
21 healthy subjects, 23 untreated thyrotoxic subjects, 13 untreated men with periodic paralysis, seven treated thyrotoxic subjects, and six treated men with periodic paralysis.


METHODS
Platelet Na+, K(+)-ATPase activity and plasma rubidium concentration after oral loading.


RESULTS
Median (range) platelet Na+, K(+)-ATPase activity in thyrotoxic subjects was 253 (169-821) mumol inorganic phosphate/h/g protein--significantly higher than that in healthy subjects (134 (81-180) mumol/h/g protein; p less than 0.001). Na+, K(+)-ATPase activity in those with periodic paralysis was 374 (195-1196) mumol/h/g protein, again significantly higher than that in healthy subjects (p less than 0.001) and that in other thyrotoxic subjects (p less than 0.01) despite similar degrees of hyperthyroidism. Activities in treated thyrotoxic subjects with and without periodic paralysis were 148 (110-234) and 131 (86-173) mumol/h/g protein respectively. Mean (95% confidence interval) plasma rubidium concentration five hours after oral administration in thyrotoxic subjects (7.0 (6.6 to 7.5) mumol/l) was significantly lower than in healthy subjects (10.2 (9.5 to 10.9) mumol/l; p less than 0.001) and higher than in those with periodic paralysis (6.0 (5.7 to 6.3) mumol/l; p less than 0.01).


CONCLUSIONS
Sodium pump activity in untreated subjects with periodic paralysis is higher than in other thyrotoxic subjects, and this may be responsible for the hypokalaemia.
Chan
A
A
Department of Chemical Pathology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT.
Shinde
R
R
Chow
C C
CC
Cockram
C S
CS
Swaminathan
R
R
D000328:Adult; D001792:Blood Platelets / Q000201:enzymology; D006801:Humans; D008297:Male; D010243:Paralysis / Q000097:blood / Q000201:enzymology*; D010507:Periodicity; D012413:Rubidium / Q000097:blood; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism* / Q000502:physiology*; D013971:Thyrotoxicosis / Q000097:blood / Q000201:enzymology*; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
D014284:Triiodothyronine; D013972:Thyrotropin; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium; D013974:Thyroxine