Activation of feline immunodeficiency virus long terminal repeat by feline herpesvirus type 1.
By transfection of a recombinant plasmid containing the feline immunodeficiency virus (FIV) long terminal repeat (LTR) linked to the chloramphenicol acetyltransferase (CAT) gene followed by infection of feline herpesvirus type 1 (FHV-1) into Crandell feline kidney cells and Felis catus whole fetus 4 cells, enhancement of CAT activity was demonstrated. Furthermore, individual feline T-lymphocytes were productively co-infected with both FIV and FHV-1 in vitro as determined by two-color immunofluorescence and electron microscopy analyses. These results revealed the transactivation of the FIV LTR by FHV-1 and the dual infection of T-lymphocytes with both viruses. The possibility that FHV-1 might be a cofactor which plays a role in the pathogenesis of FIV infection is discussed.
Kawaguchi
Y
Y
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Miyazawa
T
T
Horimoto
T
T
Itagaki
S
S
Fukasawa
M
M
Takahashi
E
E
Mikami
T
T
D000818:Animals; D015704:CD4 Antigens / Q000502:physiology; D002415:Cats; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D006564:Herpesviridae / Q000235:genetics* / Q000648:ultrastructure; D016089:Immunodeficiency Virus, Feline / Q000235:genetics* / Q000648:ultrastructure; D007700:Kinetics; D008854:Microscopy, Electron; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection
D015704:CD4 Antigens; D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase