title: A metabolite of halothane covalently binds to an endoplasmic reticulum protein that is highly homologous to phosphatidylinositol-specific phospholipase C-alpha but has no activity.
abstract: When the inhalation anesthetic halothane was administered to rats, a 58 kDa protein in the liver became covalently labeled by the trifluoroacetyl chloride metabolite of halothane. The amino acid sequences of the N-terminal and of several internal peptide fragments of the protein were 99% homologous to that of the deduced amino acid sequence of a cDNA reported to correspond to phosphatidylinositol-specific phospholipase C-alpha. The purified trifluoroacetylated 58 kDa protein or native 58 kDa protein, however, did not have phosphatidylinositol-specific phospholipase C activity. We conclude that the reported cDNA of phosphatidylinositol-specific phospholipase C-alpha may encode for a microsomal protein of unknown function.
authors.affiliation: Laboratory of Chemical Pharmacology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: J L
authors.initials: JL
authors.lastname: Martin
authors.forename: N R
authors.initials: NR
authors.lastname: Pumford
authors.forename: A C
authors.initials: AC
authors.lastname: LaRosa
authors.forename: B M
authors.initials: BM
authors.lastname: Martin
authors.forename: H M
authors.initials: HM
authors.lastname: Gonzaga
authors.forename: M A
authors.initials: MA
authors.lastname: Beaven
authors.forename: L R
authors.initials: LR
authors.lastname: Pohl
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001711:Biotransformation; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006221:Halothane / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
chemical_list: D008565:Membrane Proteins; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D006221:Halothane