title: Pharmacology of excitatory amino acid neurotransmission in nucleus laminaris of the chick.
abstract: The receptors mediating excitatory neurotransmission from the cochlear nucleus (nuc. magnocellularis, NM) to third-order auditory neurons in nucleus laminaris (NL) of the chicken were studied using in vitro brain slices, bath application of drugs, and electrophysiological recording of postsynaptic field potentials. Postsynaptic responses in NL were blocked completely, in a concentration-dependent and reversible fashion, by bath application of the broad-spectrum excitatory amino acid (EAA) antagonist kynurenic acid, the 'non-NMDA' EAA receptor antagonists 6,7-dinitroquinoxaline-2,3-dione (DNQX) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(F)quinoxaline (NBQX), and the EAA agonists domoic acid, kainic acid, alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA), and quisqualic acid. The selective NMDA receptor antagonists 3-[-)-2-carboxypiperazin-4-yl)propyl-1-phosphonate (CPP) and dibenzocycloheptenimine (MK-801) had no effect. The results demonstrate that excitatory input from the cochlear nucleus to NL is mediated by non-NMDA (G2) EAA receptors which exhibit some of the pharmacologic features typical of the AMPA receptors defined by binding studies.
authors.affiliation: Department of Anatomy, University of Utah School of Medicine, Salt Lake City 84132.
authors.forename: N
authors.initials: N
authors.lastname: Zhou
authors.forename: T N
authors.initials: TN
authors.lastname: Parks
mesh_terms: D000596:Amino Acids / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001306:Auditory Pathways / Q000502:physiology*; D001921:Brain / Q000502:physiology; D002642:Chick Embryo; D003051:Cochlea / Q000502:physiology; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors; D009847:Olivary Nucleus / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D000596:Amino Acids; D016202:N-Methylaspartate