[The effect of cyclic nucleotides on the specific binding of estradiol by skin fibroblasts in the normal state and in systemic scleroderma].
Specific binding of estradiol E2 was studied in homogenates of cultivated skin fibroblasts from healthy persons and patients with systemic sclerodermia as well as effect of cAMP and cGMP on the hormone binding was evaluated. Binding of estradiol E2 was decreased 4-fold in systemic sclerodermia. cAMP and cGMP were of importance in activation of the estradiol specific binding. Under conditions of normal state cAMP 10(-6) elevated 2-fold the estradiol specific binding, while the stimulation was not observed in systemic sclerodermia. However, cGMP 10(-6) M did not affect distinctly the estradiol E2 binding by fibroblasts of healthy volunteers but stimulated the hormone binding in systemic sclerodermia as a result of which the binding values were increased practically up to the basal level of normal state.
Kashnikova
L N
LN
Iaroshchuk
G V
GV
Panasiuk
A F
AF
Grozdova
M D
MD
D000293:Adolescent; D000328:Adult; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D012016:Reference Values; D012595:Scleroderma, Systemic / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D004958:Estradiol; D000242:Cyclic AMP; D006152:Cyclic GMP
Vliianie tsiklicheskikh nukleotidov na spetsificheskoe sviazyvanie éstradiola fibroblastami kozhi v norme i pri sistemnoĭ sklerodermii.