title: Physical map of chromosomal and plasmid DNA comprising the genome of Leptospira interrogans.
abstract: The size and physical structure of the Leptospira interrogans genome was characterized using contour-clamped homogenous electric field (CHEF) gel electrophoresis. The L. interrogans genome is approximately 4750 kb in size and is composed of two molecular species of DNA: a 4400 kb chromosome; and a 350 kb plasmid, pLIN1. A physical map of the chromosome was constructed with the restriction enzymes NotI and SfiI. A physical map of pLIN1 was constructed with ApaI, NotI, Sse83871, SgrAI, and SmaI. Both the L. interrogans chromosome and pLIN1 are circular.
authors.affiliation: Leptospirosis and Mycobacteriosis Research Unit, US Department of Agriculture, Ames, IA 50010.
authors.forename: R L
authors.initials: RL
authors.lastname: Zuerner
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D007921:Leptospira interrogans / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes