title: Effects of an essential fatty acid-supplemented diet on leukotriene B4-induced rat neutrophil functions.
abstract: This study evaluated the effect of dietary supplementation with the essential fatty acid linoleic acid to 10% of the energy content of a diet on the stimulus-response coupling of rat peritoneal neutrophils. When stimulated with leukotriene B4 neutrophils from essential fatty acid supplemented rats responded with a significantly more pronounced oxidative metabolism (assessed as luminol augmented chemiluminescence) relative to control cells from rats on a normal 3% of total energy essential fatty acid diet. Chemiluminescence response to the formylpeptide N-formyl-norleucyl-leucyl-phenylalanine-norleucyl-thyrosyl-leucine was similarly enhanced. In contrast, responses elicited by the lectin concanavalin A did not differ between the two dietary groups. In response to leukotriene B4 a dose-related inhibition of neutrophil aggregation was observed, whereas chemotaxis did not differ between the two groups. Thus, linoleate supplementation is associated with a stimulus-specific modulation of neutrophil oxidative and aggregatory responses suggesting an effect on early, conceivably receptor-linked, steps of the stimulus-response coupling.
authors.affiliation: Department of Medicine 3, Karolinska Institute at Södersjukhuset, Stockholm, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Gyllenhammar
authors.forename: J
authors.initials: J
authors.lastname: Palmblad
authors.forename: B
authors.initials: B
authors.lastname: Ringertz
mesh_terms: D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D007975:Leukotriene B4 / Q000494:pharmacology*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000008:administration & dosage*; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D009842:Oligopeptides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D008041:Linoleic Acids; D009842:Oligopeptides; D003208:Concanavalin A; D007975:Leukotriene B4; C021642:F-chemotactic peptide; D019787:Linoleic Acid