title: Detection of HHV-6 by the polymerase chain reaction.
abstract: The polymerase chain reaction (PCR) was used to detect human herpes virus 6 (HHV-6) sequences in tissue culture. A pair of primers was synthesized and used to amplify a conserved region of the genome. Amplified products were detected either by visualization of UV illuminated ethidium bromide stained gel or, by hybridization with a specific radiolabeled oligonucleotide. As little as 5 fg of HHV-6 could be detected in infected cells, making this assay suitable for diagnostic purposes.
authors.affiliation: Institut Pasteur Unité d'Oncologie Virale, Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Collandre
authors.forename: J T
authors.initials: JT
authors.lastname: Aubin
authors.forename: H
authors.initials: H
authors.lastname: Agut
authors.forename: J M
authors.initials: JM
authors.lastname: Béchet
authors.forename: L
authors.initials: L
authors.lastname: Montagnier
mesh_terms: D001483:Base Sequence; D046508:Culture Techniques; D004279:DNA, Viral / Q000032:analysis; D004996:Ethidium; D006566:Herpesviridae Infections / Q000175:diagnosis; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
chemical_list: D004279:DNA, Viral; D004996:Ethidium