Effects of ethanol on cAMP production in murine embryonic palate mesenchymal cells.
Ethanol affected the ability of murine embryonic palate mesenchymal (MEPM) cells to produce cAMP in response to hormone treatment. Acute exposure to ethanol resulted in an increase in hormone-stimulated cAMP levels, while chronic ethanol treatment led to decreased sensitivity to hormone. Forskolin-stimulated cAMP levels were decreased by both acute and chronic ethanol treatment, while the cells' response to cholera toxin was unchanged by ethanol treatment. The lack of sensitivity of the cholera toxin response to ethanol suggests that, in contrast to what has been observed in other systems, ethanol does not affect the production or activity of G alpha s in MEPM cells. These results suggest a possible explanation for the molecular basis for the craniofacial abnormalities observed in the fetal alcohol syndrome.
Weston
W M
WM
Department of Anatomy, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107.
Greene
R M
RM
D000818:Animals; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D015232:Dinoprostone / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D010159:Palate / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
D000431:Ethanol; D002772:Cholera Toxin; D000242:Cyclic AMP; D015232:Dinoprostone; D007545:Isoproterenol