title: High performance liquid chromatographic analysis of tetroxoprim and sulphadiazine in serum and urine.
abstract: Quantitative determination of tetroxoprim and sulphadiazine in serum and urine was performed using reversed phase high performance liquid chromatography. Protein precipitation using 10% perchloric acid was utilized for purification of serum samples while urine samples were diluted prior to analysis. The mobile phase consisted of triethylammonium acetate buffer (85%), acetonitrile (12%) and methanol (3%), with a final pH of 4.2. The eluent was monitored at 280 nm. Benzoic acid was used as an internal standard. Standardization, validation and application of the method is described.
authors.affiliation: Faculty of Pharmacy, Jordan University of Science and Technology, Irbid.
authors.forename: H N
authors.initials: HN
authors.lastname: Alkaysi
authors.forename: M S
authors.initials: MS
authors.lastname: Salem
authors.forename: A A
authors.initials: AA
authors.lastname: Badwan
mesh_terms: D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D010472:Perchlorates; D011743:Pyrimidines / Q000032:analysis* / Q000097:blood / Q000652:urine; D011786:Quality Control; D013411:Sulfadiazine / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D010472:Perchlorates; D011743:Pyrimidines; D013411:Sulfadiazine; C023507:tetroxoprim