A simple spectroscopic method for studying erythrocyte ghost resealing.
A novel spectroscopic method is described for following the kinetics of resealing of hemolysed erythrocyte ghosts. The procedure is based on the broadening of the EPR spectrum of nitroxyl radicals by paramagnetic ions. The method is used to study the effect of Ca2+, Mg2+ and dimethonium ion on the kinetics of resealing.
Kahana
E
E
Department of Hematology, Rambam Medical Center, Haifa, Israel.
Streichman
S
S
Silver
B L
BL
D008725:Bis-Trimethylammonium Compounds / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D004910:Erythrocyte Membrane / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology
D008725:Bis-Trimethylammonium Compounds; C047062:dimethonium; D008274:Magnesium; D002118:Calcium