Sequence and tissue-specific expression of a putative peroxidase gene from wheat (Triticum aestivum L.).
We have used a cDNA clone encoding a pathogen-induced putative wheat peroxidase to screen a genomic library of wheat (Triticum aestivum L. cv. Cheyenne) and isolated one positive clone, lambda POX1. Sequence analysis revealed that this clone contains a gene encoding a putative peroxidase with a calculated pI of 8.1 which exhibits 58% and 83% sequence identity to the amino acid sequence of the turnip (Brassica rapa) peroxidase and a pathogen-induced putative wheat peroxidase, respectively. The two introns in the wheat gene are at the same positions as introns in the peroxidase genes of tomato and horseradish. Results of S1-mapping experiments suggest that this gene is neither pathogen- nor wound-induced in leaves but is constitutively expressed in roots.
Hertig
C
C
Institute for Plant Biology, University of Zürich, Switzerland.
Rebmann
G
G
Bull
J
J
Mauch
F
F
Dudler
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D015870:Gene Expression; D007438:Introns; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000235:genetics*; D010544:Peroxidases / Q000235:genetics*; D014158:Transcription, Genetic; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
D010544:Peroxidases; C070865:wheat peroxidase; D009195:Peroxidase