[The development and use of an immunoenzyme test system for detecting and the species identification of the monkey pox virus].
An enzyme immunoassay (EIA) system for the species-specific diagnosis of monkeypox, based on the use of monoclonal antibodies (McAb) to monkeypox virus, has been developed. Immunoglobulins, isolated from McAb-containing cultural and immune ascitic fluids, have been conjugated with horse-radish peroxidase and used as detector antibodies. For immunosorption, rabbit polyclonal antibodies to the vaccine virus have been used. The specificity and sensitivity of the EIA system thus obtained have been tested on animals and humans having monkeypox and confirmed by traditional diagnostic methods (the isolation of the virus on chick embryo chorioallantoic membranes and in cell culture).
Marennikova
S S
SS
Zhukova
O A
OA
Nagieva
F G
FG
Shelukhina
E M
EM
Matsevich
G R
GR
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D002642:Chick Embryo; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D008993:Monkeypox virus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D013045:Species Specificity; D014776:Virus Cultivation / Q000379:methods
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M
Razrabotka i primenenie test-sistemy IFA dlia vyiavleniia i vidovoĭ identifikatsii virusa ospy obez'ian.