title: Purified IP3 receptor from smooth muscle forms an IP3 gated and heparin sensitive Ca2+ channel in planar bilayers.
abstract: The IP3 receptor of aortic smooth muscle, purified to near homogeneity, was incorporated into vesicle derived planar bilayers. The receptor forms channels which are gated by Ins(1,4,5)P3 (0.5 microM) and are permeable to Ca2+ (Ca2+ greater than K+ much greater than Cl-). Channel activation is specific for Ins(1,4,5)P3. Essentially no activation of channel currents was found for Ins(1,3,4)P3 or Ins(1,3,4,5)P4 at 10 microM. Heparin (25 micrograms/ml) blocked induced currents completely at all levels of activity while ATP (50 microM) increased mean current levels 2 to 4 fold. Ins(1,4,5)P3 activated mean currents increased non-linearly with voltage above about -40 mV applied voltage. Mean current levels could be reversibly adjusted by voltage to the single channel level (0 to -50 mV) or to macroscopic levels (-50 to -100 mV) over periods exceeding 1 h. Single channel events are characterized by fast transitions between predominantly non-resolved sublevels. Estimates of maximal single event currents yield a slope conductance of 32 +/- 4 pS (0 to -60 mV, 50 mM CaCl2). Thus, the purified IP3 receptor forms a channel with functional properties characteristic of IP3 triggered Ca2+ release.
authors.affiliation: Institute for Biophysics, Johannes Kepler University of Linz, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Mayrleitner
authors.forename: C C
authors.initials: CC
authors.lastname: Chadwick
authors.forename: A P
authors.initials: AP
authors.lastname: Timerman
authors.forename: S
authors.initials: S
authors.lastname: Fleischer
authors.forename: H
authors.initials: H
authors.lastname: Schindler
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D004594:Electrophysiology; D006493:Heparin / Q000494:pharmacology*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008051:Lipid Bilayers / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear
chemical_list: D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008051:Lipid Bilayers; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D006493:Heparin