Changes in serum and hepatic levels of immunoreactive prolyl hydroxylase in two models of hepatic fibrosis in rats.
Changes in serum and hepatic levels of immunoreactive prolyl hydroxylase (IRPH) as well as cellular localization of the enzyme were studied in 2 models of hepatic fibrosis, which was induced in male rats either by subcutaneous administration of CCl4 (Group A) or by intraperitoneal injection of porcine serum (Group B). Hepatic fibrosis appeared at the 8th week in Group A and at the 12th week in Group B, and liver cirrhosis developed at the 16th week in both models. Although tissue contents of hydroxyproline (HP) and IRPH increased in both models, only HP levels correlated with the degree of fibrosis. Serum IRPH levels and serum asparate aminotransferase (AST) activities increased, showing a significant positive correlation, in group A, whereas both remained in a control range in Group B. Moreover, in another model which received a single intraperitoneal injection of CCl4, serum IRPH showed a marked increase and then a rapid decrease in parallel with the change in serum AST. Immunohistochemical analysis also showed a difference between the two fibrosis models: in group A, IRPH was positive mainly in parenchymal cells in the peripheral zone of the pseudolobulus, while in group B the staining was diffuse. These results indicate that the elevation of serum IRPH is, at least in part, due to the parenchymal cell damage, and that IRPH levels should be carefully evaluated when being used as a parameter to estimate the activity of fibrogenesis in the liver.
Okuno
M
M
Department of Laboratory Medicine, Gifu University School of Medicine, Japan.
Muto
Y
Y
Kato
M
M
Moriwaki
H
H
Noma
A
A
Tagaya
O
O
Tanabe
Y
Y
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001769:Blood; D002251:Carbon Tetrachloride / Q000009:adverse effects; D006909:Hydroxyproline / Q000032:analysis; D007124:Immunoenzyme Techniques; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000097:blood / Q000201:enzymology* / Q000473:pathology; D008107:Liver Diseases / Q000097:blood / Q000201:enzymology; D008297:Male; D011393:Procollagen-Proline Dioxygenase / Q000032:analysis* / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000032:analysis; D013552:Swine; D013997:Time Factors
D012709:Serum Albumin; D002251:Carbon Tetrachloride; D011393:Procollagen-Proline Dioxygenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D006909:Hydroxyproline