Effects of 1,25-dihydroxy-vitamin D3 on phosphate accumulation by myoblasts.
The effects of 1,25-dihydroxy-Vitamin D3 on phosphate uptake by cultured chick embryonic muscle cells were investigated. Preincubation of primary myoblast cultures during 4-24 hours with physiological levels of 1,25(OH)2D3 resulted in a significant stimulation of velocity and total capacity of phosphate accumulation by the cells. Maximal responses were obtained at 8 hours of treatment with the sterol. In agreement with previous studies, 25-hydroxy-Vitamin D3 also stimulated myoblast phosphate uptake. 24,25-dihydroxy-Vitamin D3 and vitamin D3 were ineffective. Evidence was obtained indicating that 1,25(OH)2D3 affects the Na(+)-linked component of muscle cell phosphate uptake through a mechanism dependent on "de movo" protein and RNA synthesis.
Bellido
T
T
Departamento de Biologia, Universidad Nacional del Sur, Bahia Blanca, Argentina.
Boland
R
R
Teresita
B
B
Ricardo
B
B
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002112:Calcifediol / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D002762:Cholecalciferol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D007700:Kinetics; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D012964:Sodium / Q000494:pharmacology
D010710:Phosphates; D010761:Phosphorus Radioisotopes; D002762:Cholecalciferol; D003609:Dactinomycin; D003513:Cycloheximide; D012964:Sodium; D002117:Calcitriol; D002112:Calcifediol