Effect of chronic ethanol consumption on postnatal development of renal (Na + K)-ATPase in the rat.
Renal (Na + K)-ATPase was studied to ascertain whether it follows the pattern of adaptation of membrane-bound enzymes that are inhibited by acute ethanol exposure and develop greater activity after chronic ethanol treatment. A colony of rats was given 20 per cent (v/v) ethanol as sole drinking solution throughout gestation, lactation and following weaning. (Na + K)-ATPase and ouabain-insensitive Ca(2+)-ATPase activities were determined; regional distribution of these enzymes was assessed in renal cortex and outer medulla. Control rats drank tap water. (Na + K)-ATPase in whole homogenate of kidney increased with age in controls and ethanol-fed rats, but the latter showed higher values at every age studied. Between 15 and 60 days of age, the control group showed 2-fold increases in cortex and 5-fold in outer medulla, whereas ethanol-fed rats reached a 3-fold increase in the enzyme activity in both renal regions. Ca(2+)-ATPase showed the same time course in developing kidney of both groups. Chronic ethanol treatment of adult rats resulted in an increase of (Na + K)-ATPase activity in cortex and outer medulla, but no change in other ATPases. Since an earlier maturational development of renal (Na + K)-ATPase was displayed by ethanol-fed rats, underlying mechanisms that may account for these results are discussed.
Rodrigo
R
R
Department of Experimental Medicine, Faculty of Medicine, University of Chile, Santiago.
Vergara
L
L
Oberhauser
E
E
D000251:Adenosine Triphosphatases / Q000187:drug effects*; D000367:Age Factors; D000428:Alcohol Drinking / Q000009:adverse effects*; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D007668:Kidney / Q000201:enzymology*; D007672:Kidney Cortex / Q000201:enzymology; D007679:Kidney Medulla / Q000201:enzymology; D008431:Maternal-Fetal Exchange; D008565:Membrane Proteins / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects
D008565:Membrane Proteins; D000431:Ethanol; D000251:Adenosine Triphosphatases; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase