Molecular cloning and mammalian expression of human beta 2-glycoprotein I cDNA.
Human beta 2-glycoprotein (beta 2gpI) cDNA was isolated from a liver cDNA library and sequenced. The cDNA encoded a 19-residue hydrophobic signal peptide followed by the mature beta 2gpI of 326 amino acid residues. In liver and in the hepatoma cell line HepG2 there are two mRNA species of about 1.4 and 4.3 kb, respectively, hybridizing specifically with the beta 2gpI cDNA. Upon isoelectric focusing, recombinant beta 2gpI obtained from expression of beta 2gpI cDNA in baby hamster kidney cells showed the same pattern of bands as beta 2gpI isolated from plasma, and at least 5 polypeptides were visible.
Kristensen
T
T
Department of Molecular Biology and Plant Physiology, University of Aarhus, Denmark.
Schousboe
I
I
Boel
E
E
Mulvihill
E M
EM
Hansen
R R
RR
Møller
K B
KB
Møller
N P
NP
Sottrup-Jensen
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001053:Apolipoproteins / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular; D003001:Cloning, Molecular; D005260:Female; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008099:Liver / Q000502:physiology; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010920:Placenta / Q000502:physiology; D011247:Pregnancy; D011994:Recombinant Proteins / Q000302:isolation & purification; D014162:Transfection; D014407:Tumor Cells, Cultured; D053482:beta 2-Glycoprotein I
D001053:Apolipoproteins; D006023:Glycoproteins; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D053482:beta 2-Glycoprotein I