title: Characterization of a gelatinase from human rheumatoid synovial fluid cells.
abstract: A metalloproteinase with a specificity for gelatin was isolated from serum-free medium of cultures of rheumatoid synovial fluid. The enzyme showed all the properties of a leukocyte gelatinase. In addition to gelatin this proteinase cleaved the synthetic substrate dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg (Dnp-peptide) rapidly, while casein was a much poorer substrate. This proteinase showed no enzymatic activity against collagen type I, was secreted in a latent form and could be activated by trypsin or organomercurial compounds, such as mersalylic acid or 4-aminophenyl-mercury acetate. The latent enzyme had an apparent molecular mass of 130,000-150,000 estimated by gel filtration or 97,000 by electrophoresis on polyacrylamide gel containing sodium dodecyl sulphate. When analysed by immunoblotting the enzyme was recognized by antibodies raised against human polymorphonuclear leukocyte gelatinase. Although we found synovial fibroblasts to be largely present in the cell cultures we could not detect any fibroblast gelatinase activity.
authors.affiliation: Institut für Biochemie, Freie Universität Berlin.
authors.forename: H
authors.initials: H
authors.lastname: Kolkenbrock
authors.forename: H M
authors.initials: HM
authors.lastname: Ali
authors.forename: A
authors.initials: A
authors.lastname: Hecker-Kia
authors.forename: G
authors.initials: G
authors.lastname: Buchlow
authors.forename: H
authors.initials: H
authors.lastname: Sörensen
authors.forename: R W
authors.initials: RW
authors.lastname: Hauer
authors.forename: N
authors.initials: N
authors.lastname: Ulbrich
mesh_terms: D001172:Arthritis, Rheumatoid / Q000201:enzymology*; D002478:Cells, Cultured; D004140:Dinitrophenols / Q000032:analysis; D004789:Enzyme Activation; D018093:Gelatinases; D006801:Humans; D009504:Neutrophils / Q000201:enzymology; D009842:Oligopeptides / Q000032:analysis; D010434:Pepsin A / Q000032:analysis* / Q000302:isolation & purification; D013379:Substrate Specificity; D013582:Synovial Fluid / Q000166:cytology / Q000201:enzymology*
chemical_list: D004140:Dinitrophenols; D009842:Oligopeptides; C024118:DNP-peptide; D010434:Pepsin A; D018093:Gelatinases