Selenium antagonizes the induction of human heme oxygenase by arsenite and cadmium ions.
Effects of selenium compounds on the induction of heme oxygenase in human cells exposed to sodium arsenite or cadmium chloride have been investigated by an immunoblotting technique. Exposure of HeLa cells to arsenite or cadmium ions caused a marked increase in the synthesis of heme oxygenase, and the presence of sodium selenite suppressed the induction. DL-Selenocystine was an effective suppressor, and sodium selenate was less effective. DL-Selenomethionine had no effect. Northern blot analysis showed that selenite abolished the induction of heme oxygenase mRNA in the cells exposed to arsenite or cadmium ions. These results indicated that selenium antagonizes the induction of heme oxygenase by heavy metals ions.
Taketani
S
S
Department of Hygiene, Kansai Medical University, Osaka, Japan.
Kohno
H
H
Tokunaga
R
R
Ishii
T
T
Bannai
S
S
D001151:Arsenic / Q000037:antagonists & inhibitors*; D018053:Arsenites; D015152:Blotting, Northern; D002104:Cadmium / Q000037:antagonists & inhibitors*; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D006367:HeLa Cells; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D006801:Humans; D015151:Immunoblotting; D012643:Selenium / Q000494:pharmacology*; D017279:Selenocysteine; D018038:Sodium Selenite
D018053:Arsenites; D002104:Cadmium; D017279:Selenocysteine; D006419:Heme Oxygenase (Decyclizing); D012643:Selenium; D018038:Sodium Selenite; D003545:Cysteine; C015001:arsenite; D001151:Arsenic