Clinical utility of fractionating erythrocytes into "Percoll" density gradients.
Two rapid methods for fractionating the RBC into five or nine layers of increasing density are reported. These procedures have been used to monitor the decline of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activity during the process of red cell aging in normal subjects and in beta-thal carriers, to study transfused patients with G6PD and pyruvate kinase (PK) deficiency and to test the effects of inositol hexaphosphate (IHP) encapsulation on RBC subpopulations.
Mosca
A
A
Dipt. Sc. Tecnol. Biomediche, Univ. Milano.
Paleari
R
R
Modenese
A
A
Rossini
S
S
Parma
R
R
Rocco
C
C
Russo
V
V
Caramenti
G
G
Paderi
M L
ML
Galanello
R
R
D000328:Adult; D000743:Anemia, Hemolytic / Q000097:blood; D002458:Cell Fractionation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D002648:Child; D004796:Clinical Enzyme Tests; D004339:Drug Compounding; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000097:blood*; D006579:Heterozygote; D006593:Hexokinase / Q000097:blood; D006801:Humans; D008297:Male; D010734:Phosphogluconate Dehydrogenase / Q000097:blood*; D010833:Phytic Acid / Q000008:administration & dosage; D011205:Povidone; D011770:Pyruvate Kinase / Q000097:blood / Q000172:deficiency*; D012156:Reticulocytes / Q000201:enzymology; D012822:Silicon Dioxide; D013789:Thalassemia / Q000097:blood*
C016039:Percoll; D012822:Silicon Dioxide; D010833:Phytic Acid; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D011770:Pyruvate Kinase; D005634:Fructose-Bisphosphate Aldolase; D011205:Povidone