Suppression of growth-promoting activity in extract from human uterine cancer by cyclic AMP-mediated mechanism.
Uterine cervical and corpus cancers have been reported to synthesize and secrete the putative peptide mitogen, which elicits a potent proliferative response in the fibroblasts [Res. Commun. Chem. Pathol. Pharmacol. 66, 477-480 (1989)]. The extract from human cervical cancers stimulated [H]thymidine incorporation into human endometrial fibroblasts in a dose-dependent manner. Concomitant exposure of the fibroblasts to dibutyryl cyclic AMP (cAMP) of forskolin, the mitogenic activity of the extract was suppressed by 60%. A higher concentration of the extract did not overcome the inhibitory effects of them, implying that they acted at a stage after the mitogen in extract and specific receptor interaction. These results demonstrated that the growth-promoting activity in cervical cancer extract could be under negative control through cAMP-dependent mechanism.
Matsunami
K
K
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
Imai
A
A
Ohno
T
T
Tamaya
T
T
D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D016889:Endometrial Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D013936:Thymidine; D014020:Tissue Extracts / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000473:pathology; D014594:Uterine Neoplasms / Q000188:drug therapy* / Q000473:pathology
D014020:Tissue Extracts; D005576:Colforsin; D003994:Bucladesine; D000242:Cyclic AMP; D013936:Thymidine