title: Anesthetic dependence of the inhibitory effect of neurotensin on pentagastrin-stimulated acid secretion in rats. A possible role for somatostatin.
abstract: The existence of possible local mediators of the inhibitory effect of neurotensin on gastric acid secretion has not been determined. We perfused rats intragastrically with warmed saline and stimulated acid secretion with intravenous pentagastrin, 32 micrograms/kg/hr, and found that anesthesia with pentobarbital resulted in marked inhibition of acid secretion by intravenous neurotensin; however, anesthesia with urethane prevented this inhibitory effect of neurotensin from occurring. In addition, we found a significant increase in somatostatin-like immunoreactivity in portal venous blood during neurotensin infusion in pentobarbital-anesthetized rats. Neither neurotensin nor pentagastrin infusion modified gastric luminal somatostatin-like immunoreactivity after either pentobarbital or urethane, and rats anesthetized with urethane did not show an increase of somatostatin-like immunoreactivity in portal venous blood during neurotensin infusion. These results suggested that somatostatin-like immunoreactivity, released into the portal circulation, was necessary for exogenous neurotensin to inhibit pentagastrin-stimulated gastric acid secretion under these conditions in anesthetized rats.
authors.affiliation: Department of Medicine, Tulane University Medical School, VA Medical Center, New Orleans, LA 70146.
authors.forename: R A
authors.initials: RA
authors.lastname: Hammer
authors.forename: C
authors.initials: C
authors.lastname: Fernandez
authors.forename: A
authors.initials: A
authors.lastname: Ertan
authors.forename: A
authors.initials: A
authors.lastname: Arimura
mesh_terms: D000768:Anesthesia, General; D000818:Animals; D005260:Female; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects; D007700:Kinetics; D009496:Neurotensin / Q000494:pharmacology*; D010418:Pentagastrin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010424:Pentobarbital / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000502:physiology*; D014520:Urethane / Q000494:pharmacology*
chemical_list: D009496:Neurotensin; D014520:Urethane; D013004:Somatostatin; D010418:Pentagastrin; D010424:Pentobarbital