Urovirulence determinants in Escherichia coli isolates causing first-episode and recurrent cystitis in women.
To assess the prevalence of urovirulence determinants among Escherichia coli isolates from women with acute uncomplicated cystitis, 121 isolates from 87 women with first-episode or recurrent cystitis and 156 fecal isolates from 52 women without recent urinary tract infection were tested using DNA probes for P fimbriae, hemolysin, aerobactin, and diffuse adhesin and for expression of hemolysin and P and F adhesins. P fimbrial genotype (P = .002), hemolysin phenotype (P = .007), and the diffuse adhesin determinant (P = .03), but not aerobactin, were found more frequently in E. coli from women with acute cystitis, and expression of the F adhesin (41%) was more common than the P adhesin (24%; P = .001). E. coli isolates that caused cystitis in women using diaphragms had fewer virulence determinants than those from nonusers (P = .04), suggesting that diaphragm use may allow infection with less virulent E. coli.
Stapleton
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Department of Medicine, University of Washington, Seattle.
Moseley
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Stamm
W E
WE
D000208:Acute Disease; D018830:Adhesins, Escherichia coli; D000328:Adult; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D003556:Cystitis / Q000382:microbiology*; D015342:DNA Probes; D004926:Escherichia coli / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D010861:Fimbriae, Bacterial; D005838:Genotype; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D006877:Hydroxamic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012008:Recurrence; D014774:Virulence
D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D015342:DNA Probes; D006460:Hemolysin Proteins; D006877:Hydroxamic Acids; C031819:aerobactin