The isolation and characterisation of plant sequences homologous to human hypervariable minisatellites.
We have isolated DNA probes, homologous to the human hypervariable minisatellite sequence 33.15, from the genome of rice (Oryza sativa). These probes are capable of producing a multilocus rice DNA fingerprint. The rice sequence has a tandem repeating structure based on a 12 bp GC-rich repeat which shows homology to its human counterpart. This probe detects up to 30 loci which are at a number of unlinked chromosomal sites. The GC-rich sequence is invariably associated with an open reading frame (ORF) of unknown function. The ORF is probably a member of a small multigene family.
Daly
A
A
I.C.I Seeds, Jealott's Hill Research Station, Bracknell, Berkshire, Great Britain.
Kellam
P
P
Berry
S T
ST
Chojecki
A J
AJ
Barnes
S R
SR
D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012275:Oryza / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004276:DNA, Satellite; D004262:DNA Restriction Enzymes