[Detection of bovine papillomavirus DNA in equine sarcoids using the polymerase chain reaction (PCR)].
Unfixed and formalin-fixed frozen sections and paraffin-sections of histopathologically confirmed sarcoids of 20 horses were studied in the PCR. The used set of primers was located in the E5 open reading frame fitting both to bovine papillomavirus 1 (BPV-1) and BPV-2. Independent of the quality of the used tissues BPV-DNA was detected in all 20 sarcoids. By cleaving with restriction endonuclease Bst XI it was shown that the DNA-sequences amplified by PCR were identical with that of BPV-1. The results support the general view that BPV play an important role in equine sarcoids.
Teifke
J P
JP
Institut für Veterinärpathologie, Justus-Liebig-Universität Giessen.
Weiss
E
E
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000382:microbiology / Q000662:veterinary*; D014412:Tumor Virus Infections / Q000382:microbiology / Q000662:veterinary*
D004279:DNA, Viral
Nachweis boviner Papillomvirus-DNA in Sarkoiden des Pferdes mittels der Polymerase-Kettenreaktion (PCR).