Cloning and expression of human endothelin-1 receptor cDNA.
We isolated a human endothelin-1 (ET-1) receptor cDNA from a human placenta cDNA library. The cDNA encodes a 427-amino acid protein with seven putative transmembrane domains. The rank order of the binding to the receptor expressed in COS-7 cells was: ET-1 greater than or equal to ET-2 much greater than ET-3. The receptor expressed in Xenopus oocytes showed a potent electrophysiological response to 1 x 10(-7) M ET-1 under voltage clamp at -60 mV, while a much weaker response was produced by 1 x 10(-7) M ET-3. Northern blot analysis with RNA from human tissues revealed a single band with a size of 4.3 kb in a wide variety of human tissues, especially highly in the blood vessel.
Hosoda
K
K
Department of Medicine, Kyoto University School of Medicine, Japan.
Nakao
K
K
Hiroshi-Arai
Suga
S
S
Ogawa
Y
Y
Mukoyama
M
M
Shirakami
G
G
Saito
Y
Y
Nakanishi
S
S
Imura
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004594:Electrophysiology; D016232:Endothelins / Q000378:metabolism / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D010920:Placenta / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D017466:Receptors, Endothelin; D014162:Transfection; D014981:Xenopus
D016232:Endothelins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D004247:DNA