Characterization of an avian rotavirus strain by neutralization and molecular hybridization assays.
Pigeon rotavirus strain PO-13, which was recently shown to be neutralized by a hyperimmune serum to the prototype serotype 7 virus Ch-2, showed a one-way neutralization cross with turkey rotavirus Ty-1. When its genome was compared by RNA-RNA hybridization under stringent conditions with those of avian and mammalian rotaviruses, PO-13 displayed a low to medium level of homology only with turkey rotavirus strains Ty-1 and Ty-3 but not with chicken rotavirus strain Ch-1. Furthermore, no homology was found between the PO-13 probe and genomic RNA from 11 rotavirus strains which originated from 6 different mammalian species and which represented 6 major mammalian serotypes (1-6).
Minamoto
N
N
Department of Veterinary Public Health, Faculty of Agriculture, Gifu University, Japan.
Nakagomi
O
O
Sugiyama
M
M
Kinjo
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D010856:Columbidae / Q000382:microbiology*; D016679:Genome, Viral; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012367:RNA, Viral / Q000235:genetics*; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D012703:Serotyping; D014422:Turkeys / Q000382:microbiology
D000914:Antibodies, Viral; D015347:RNA Probes; D012367:RNA, Viral