Chemotactic peptides but not macrophage colony stimulating factor effect the synthesis of inositol lipids in macrophages.
Normal bone marrow derived macrophages display a wide variety of biological responses to a number of distinct agonists, for example, Macrophage Colony Stimulating Factor (M-CSF) and chemotactic peptides (such as FMLP). FMLP stimulates reactive oxygen intermediate production in these cells, whilst M-CSF stimulates DNA synthesis. We have compared the effects of these two agents on the production of novel inositol lipids in macrophages. Evidence is presented that FMLP, but not M-CSF elevate the levels of a lipid putatively identified as phosphatidylinositol-3,4-bisphosphate. The implications of this observation on proposed role of novel inositol lipids in macrophage proliferation are discussed.
Vallance
S J
SJ
Department of Biochemistry and Applied Molecular Biology, UMIST, Manchester, UK.
MacPhee
C H
CH
Downes
C P
CP
Whetton
A D
AD
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D018129:Phosphatidylinositol Phosphates / Q000096:biosynthesis; D010716:Phosphatidylinositols / Q000096:biosynthesis*
D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; C060980:phosphatidylinositol 3,4-diphosphate; D009240:N-Formylmethionine Leucyl-Phenylalanine; D016173:Macrophage Colony-Stimulating Factor