title: Electrophysiological interactions between NMDA and phencyclidine/sigma receptor agonists and antagonists in Purkinje neurons in the cerebellum of the rat.
abstract: The purpose of this experiment was to study the electrophysiological interactions between phencyclidine and NMDA receptor agonists. Drugs were directly applied to cerebellar Purkinje neurons of urethane-anesthetized rats, through a multibarreled pipette by pressure ejection. Neuronal activity was recorded extracellularly. In almost all the cells encountered, NMDA-induced responses were stereospecifically blocked by phencyclidine/sigma agonists, such as: (+)PCMP[1-(1-phenyl cyclohexyl)-3-methyl piperidine] or dexoxadrol. However, quisqualate-mediated excitation was also antagonized by (+)PCMP, although quisqualate had a lower sensitivity to (+)PCMP than NMDA did. It was previously found that metaphit irreversibly blocked phencyclidine-induced responses in the cerebellum. It was found that metaphit attenuated phencyclidine-induced antagonism of NMDA responses. In conclusion, these findings suggested that phencyclidine/sigma agonists antagonized effects of NMDA and quisqualate, whereas the presence of an intact phencyclidine/sigma receptor was not essential for the NMDA-mediated response in the cerebellum.
authors.affiliation: Department of Pharmacology, National Defense Medical Center, Taipei, Taiwan, Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: H K
authors.initials: HK
authors.lastname: Lee
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000378:metabolism; D008297:Male; D016202:N-Methylaspartate / Q000008:administration & dosage / Q000494:pharmacology*; D009991:Oscillometry; D010622:Phencyclidine / Q000008:administration & dosage / Q000494:pharmacology*; D011689:Purkinje Cells / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D017981:Receptors, Neurotransmitter / Q000187:drug effects; D011957:Receptors, Opioid / Q000187:drug effects*; D017478:Receptors, Phencyclidine; D017480:Receptors, sigma
chemical_list: D017981:Receptors, Neurotransmitter; D011957:Receptors, Opioid; D017478:Receptors, Phencyclidine; D017480:Receptors, sigma; D016202:N-Methylaspartate; D010622:Phencyclidine