title: gIII antibody responses in pigs following vaccination and challenge to Aujeszky's disease.
abstract: Serological responses to a genetically engineered Aujeszky's disease "marker" vaccine (dl gIII + dl tk) were monitored using a blocking-ELISA (B-ELISA), a serum neutralisation test (SNT) and an indirect ELISA (I-ELISA). The B-ELISA is capable of differentiating pigs vaccinated with the above vaccine from natural infection. The SNT and the I-ELISA indicated that the pigs responded to vaccination and challenge. All three tests showed that the controls and the in-contact pigs always reacted negative for antibodies. The B-ELISA was able to detect pigs challenged with a field isolate 24 days post-challenge. These pigs remained positive until 110 days post-challenge when last tested. These findings indicate that the B-ELISA could be used successfully with this vaccine in a control eradication programme. This trial also shows that the vaccine virus did not spread to the in-contact pigs and also the vaccinated and challenged pigs did not transmit the disease to other susceptible pigs when they were introduced 14 days after challenge.
authors.affiliation: MAF Quality Management, Central Animal Health Laboratory, Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
authors.forename: M X
authors.initials: MX
authors.lastname: Motha
authors.forename: J C
authors.initials: JC
authors.lastname: Ralston
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D009500:Neutralization Tests; D011557:Pseudorabies / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic; D014765:Viral Vaccines