Human cytomegalovirus structural components: intracellular and intraviral localization of p38.
Rabbit antisera raised against the product of ORF UL 80 of human cytomegalovirus (CMV) genome (HindIII L fragment of AD169 strain) as well as IgM from acutely infected patients recognize an antigen of Mr 38 kDa. In the viral particle this antigen is bound via S-S bridge to a lower Mr compound to form a final complex of 62 kDa that is also recognized by rabbit antisera as well as patients' IgM. P38 is present both in the nucleus and in the cytoplasm of CMV-infected cells starting from 24 h p.i. and increasingly thereafter. Immunoelectron microscopy revealed that this antigen is mainly associated with the internal portion of viral capsids both in the nucleus and in the cytoplasm.
Landini
M P
MP
Institute of Microbiology, Faculty of Medicine, University of Bologna, Italy.
Severi
B
B
Cenacchi
G
G
Lazzarotto
T
T
Lindenmeier
W
W
Necker
A
A
D000956:Antigens, Viral / Q000737:chemistry / Q000378:metabolism; D002213:Capsid / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D002467:Cell Nucleus / Q000382:microbiology; D003587:Cytomegalovirus / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D003586:Cytomegalovirus Infections / Q000382:microbiology; D003593:Cytoplasm / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D015678:Viral Structural Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
D000956:Antigens, Viral; D015678:Viral Structural Proteins