Analysis by mRNA levels of the expression of six G protein alpha-subunit genes in mammalian cells and tissues.
The distribution and levels of expression of Gs alpha, Gi1 alpha, Gi2 alpha, Gi3 alpha, Go alpha, and Gx alpha mRNAs were compared by Northern blot analysis using several rat tissues and selected human and rat cell lines. Gi1 alpha, Go alpha, and Gx alpha, were detected in a limited number of tissue and cells whereas Gi2 alpha, Gi3 alpha, and Gs alpha, were expressed in all the tissues and cells tested albeit in varying amounts. The expression of these six genes appears to be differentially regulated during postnatal development of the rat brain. High expression levels particularly of Go alpha, in young rat brain may be related to the formation of neurites during differentiation of nerve cells.
Garibay
J L
JL
Institute of Medical Science, University of Tokyo, Japan.
Kozasa
T
T
Itoh
H
H
Tsukamoto
T
T
Matsuoka
M
M
Kaziro
Y
Y
D000367:Age Factors; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000502:physiology; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D009414:Nerve Growth Factors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014018:Tissue Distribution
D009414:Nerve Growth Factors; D012333:RNA, Messenger; D003994:Bucladesine; D019204:GTP-Binding Proteins