title: Identification of 3',5'-cyclic AMP in axenic cultures of Lemna paucicostata by high-performance liquid chromatography.
abstract: Nucleotides were purified from the extract of aseptically grown Lemna paucicostata 6746 by low-pressure column chromatography and thin-layer chromatography. In this partially purified sample, a compound has been identified, using a highly sensitive HPLC-fluorometric detection method, with chromatographic properties and substrate specificity for cyclic nucleotide phosphodiesterase similar to authentic 3',5'-cyclic AMP. The level of cAMP has been estimated to be 70-80 pmol/g fresh wt.
authors.affiliation: Department of Plant Molecular Biology, University of Delhi, India.
authors.forename: L
authors.initials: L
authors.lastname: Gangwani
authors.forename: B K
authors.initials: BK
authors.lastname: Tamot
authors.forename: J P
authors.initials: JP
authors.lastname: Khurana
authors.forename: S C
authors.initials: SC
authors.lastname: Maheshwari
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000242:Cyclic AMP / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014316:Tritium
chemical_list: D014316:Tritium; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases