Ac-induced disruption of the double Ds structure in tomato.
The maize doubleDs element is stably maintained in the tomato genome. Upon the subsequent introduction of Ac into a plant containing doubleDs, disruption of the doubleDs structure and DNA rearrangements at the site of the doubleDs element were observed. No indications were obtained for excision of the complete doubleDs structure. The consequences of transactivation of doubleDs in these experiments are different from those described for transactivation of single Ds elements in tomato. The mechanisms by which such rearrangements could have occurred in tomato are discussed in relation to complex insertions containing doubleDs in maize.
Rommens
C M
CM
Free University, Department of Genetics, Amsterdam, The Netherlands.
van der Biezen
E A
EA
Ouwerkerk
P B
PB
Nijkamp
H J
HJ
Hille
J
J
D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D005638:Fruit / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015533:Transcriptional Activation; D003313:Zea mays / Q000235:genetics
D004251:DNA Transposable Elements; D004247:DNA