Light-dependent spin trapping of hydroxyl radical from human erythrocytes.
The generation of reactive oxygen species from human erythrocytes has previously been demonstrated. Furthermore, erythrocytic protoporphyrin IX has been shown to generate superoxide and singlet oxygen when exposed to light. These findings suggest that a component of erythrocytic reactive oxygen species production may be light-dependent. By inhibiting erythrocyte superoxide dismutase, catalase, and glutathione peroxidase with N,N-diethyldithiocarbamate or sodium cyanide, we demonstrate the light-dependent generation of hydroxyl radical in human erythrocytes using spin trapping/Electron Spin Resonance spectroscopy. This finding may be significant in tissues where blood is exposed to light, such as in the eye.
Bynoe
L A
LA
Wilmer Institute, Johns Hopkins Hospital, Baltimore, Md. 21205.
Pou
S
S
Gottsch
J D
JD
Rosen
G M
GM
D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D003497:Cyclic N-Oxides / Q000494:pharmacology; D004050:Ditiocarb / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005609:Free Radicals; D006801:Humans; D006878:Hydroxides / Q000097:blood*; D017665:Hydroxyl Radical; D066298:In Vitro Techniques; D007700:Kinetics; D008027:Light; D004369:Pentetic Acid / Q000494:pharmacology; D012966:Sodium Cyanide / Q000494:pharmacology
D003497:Cyclic N-Oxides; D005609:Free Radicals; D006878:Hydroxides; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D017665:Hydroxyl Radical; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D004369:Pentetic Acid; D004050:Ditiocarb; D012966:Sodium Cyanide