[Restoration of the potential-dependent L-phenylalanine-inhibited calcium current by L-tyrosine in cultured hippocampal neurons].
Calcium currents were recorded in cultured (5-7 days) hippocampal neurons isolated from one-day rats. The animals were intraperitoneally injected L-phenylalanine which induced in their brain biochemical changes typical of phenylketonuria. It has been found that in neurons from injected animals the amplitude of the high-threshold calcium current was substantially inhibited and amounted to 40 +/- 30% at Vt = +20 mV (amplitude of calcium currents at Vt = -10 mV taken as 100%). Addition of L-tyrosine to the cultivation medium (50 mumol/l) restored the high-voltage calcium current, its relative amplitude reaching 280 +/- 57%.
Martyniuk
A E
AE
Savina
S V
SV
Skibo
G G
GG
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D015220:Calcium Channels / Q000187:drug effects*; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D010649:Phenylalanine / Q000037:antagonists & inhibitors*; D051381:Rats; D014443:Tyrosine / Q000494:pharmacology*
D015220:Calcium Channels; D014443:Tyrosine; D010649:Phenylalanine
Vosstanovlenie L-tirozinom potentsialozavisimogo kal'tsievogo toka, podavlennogo L-fenilalaninom, v kul'tiviruemykh neĭronakh gippokampa.