Brain tumors predominantly express the neurofibromatosis type 1 gene transcripts containing the 63 base insert in the region coding for GTPase activating protein-related domain.
Neurofibromatosis type 1 (NF1) is an autosomal dominant neurocutaneous disorder. A part of the gene for NF1 was cloned and its deduced protein has a domain functionally related to mammalian ras GTP-ase-activating protein (GAP). Human tissues examined express two types of NF1 mRNAs: an originally identified species of NF1 mRNA (type I) and another one containing the 63 base insert in the region coding for GAP-related domain (type II). However relative levels of both mRNAs seem to change under certain conditions. Human brain expresses type I mRNA predominantly, while type II is preferentially expressed in most primary brain tumors (13/16 tumors analyzed). We suggest that higher levels of type II mRNA may be related to the genesis of brain tumors.
Suzuki
Y
Y
Department of Applied Physiology and Molecular Biology, Tohoku University School of Medicine, Miyagi, Japan.
Suzuki
H
H
Kayama
T
T
Yoshimoto
T
T
Shibahara
S
S
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D001483:Base Sequence; D001932:Brain Neoplasms / Q000145:classification / Q000235:genetics*; D002648:Child; D004251:DNA Transposable Elements; D005260:Female; D020690:GTPase-Activating Proteins; D015972:Gene Expression Regulation, Neoplastic; D016514:Genes, Neurofibromatosis 1; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D014158:Transcription, Genetic; D020703:ras GTPase-Activating Proteins
D004251:DNA Transposable Elements; D020690:GTPase-Activating Proteins; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D020703:ras GTPase-Activating Proteins; D006419:Heme Oxygenase (Decyclizing)