Diagnosis of human cytomegalovirus-induced retinitis in human immunodeficiency virus type 1-infected subjects by using the polymerase chain reaction.
In 13 of 16 AIDS patients with retinitis, a herpesviruslike infection was diagnosed by clinical investigation. In 12 of the 13 patients, human cytomegalovirus (HCMV) DNA was detected in 5 microliters of aqueous humor by using the polymerase chain reaction (PCR). In the aqueous humor of 12 control patients HCMV DNA could not be detected by PCR. PCR may be used to monitor specific antiviral long-term therapy in HCMV retinitis.
Fenner
T E
TE
Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Garweg
J
J
Hufert
F T
FT
Boehnke
M
M
Schmitz
H
H
D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000175:diagnosis; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015658:HIV Infections / Q000150:complications*; D015497:HIV-1; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009894:Opportunistic Infections / Q000150:complications* / Q000175:diagnosis; D016133:Polymerase Chain Reaction; D012173:Retinitis / Q000150:complications* / Q000175:diagnosis
D004279:DNA, Viral; D015345:Oligonucleotide Probes