Isolation in Italy of feline immunodeficiency virus (FIV).
The Authors report the isolation in Italy of Feline Immunodeficiency Virus (FIV) from a cat inoculated with whole blood from a naturally FIV infected cat. The virus was isolated in feline circulating leucocytes cultured in RPMI medium and stimulated with concanavalin-A and recombinant human interleukin-2. The infected cultures showed a characteristic cytopathic effect (ballooning degeneration, giant cell formation, cell death) and a specific fuorescence using FIV-positive cat serum and monoclonal antibodies against FIV. Furthermore, the culture supernatants contained magnesium-dependent reverse transcriptase activity.
Buonavoglia
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C
Istituto di Patologia delle Malattie Infettive e Parassitarie degli animali domestici, Facoltà di Medicina Veterinaria, Bari.
Tempesta
M
M
Pennisi
M G
MG
Di Trani
L
L
Marsilio
F
F
Titti
F
F
Catarsini
O
O
Compagnucci
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D002415:Cats; D002470:Cell Survival; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D016181:Feline Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D016089:Immunodeficiency Virus, Feline / Q000276:immunology / Q000302:isolation & purification*; D007376:Interleukin-2 / Q000494:pharmacology; D007558:Italy; D007962:Leukocytes / Q000382:microbiology*; D011994:Recombinant Proteins / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D011994:Recombinant Proteins; D003208:Concanavalin A