Cloning and characterization of the gene encoding rabbit cardiac calsequestrin.
A cDNA encoding rabbit cardiac calsequestrin was isolated and characterized. The deduced nascent cardiac calsequestrin contains 409 amino acids of which 26% are acidic residues, and had 93% and 67% aa identity with canine cardiac calsequestrin and rabbit fast-twitch skeletal muscle calsequestrin, respectively. RNA blot analyses indicate that this mRNA is expressed in atrium, ventricle and to a lesser amount in slow-twitch skeletal muscle. This mRNA transcript is not expressed in adult fast-twitch skeletal muscle, smooth muscle, or nonmuscle tissues. Analysis of in vitro skeletal muscle myogenesis using a mouse myoblast cell line C2C12, demonstrates that both cardiac and skeletal calsequestrin isoforms are coproduced during muscle differentiation.
Arai
M
M
Department of Physiology and Biophysics, University of Vermont College of Medicine, Burlington 05403.
Alpert
N R
NR
Periasamy
M
M
D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002155:Calsequestrin / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D006321:Heart / Q000254:growth & development; D008969:Molecular Sequence Data; D024510:Muscle Development; D009124:Muscle Proteins / Q000235:genetics; D009132:Muscles / Q000737:chemistry* / Q000166:cytology; D009206:Myocardium / Q000737:chemistry* / Q000166:cytology; D018008:Myogenin; D011817:Rabbits
D000199:Actins; D002155:Calsequestrin; D009124:Muscle Proteins; D018008:Myogenin; D004247:DNA