title: Localization of rhinovirus replication in vitro with in situ hybridization.
abstract: To facilitate understanding of human rhinovirus (HRV) pathogenesis, methods were developed for detection of HRV infection in vitro using in situ hybridization (ISH). HRV-14 RNA probes and oligonucleotide probes representing conserved sequences in the 5'-non-translated region were labeled with 35S and used to detect infected HeLa or WI-38 strain human embryonic lung cells in cytological preparations. ISH was shown to be specific for detection of HRV on a single-cell basis. Subsequently, in human nasal polyps infected in vitro, both oligonucleotide- and ribo-probes produced a strong signal in association with ciliated epithelial cells. In human adenoids infected in vitro, a signal was observed in non-ciliated epithelial cells. This study shows that HRV replicates in ciliated cells in the epithelium of human nasal polyps infected in vitro, and the presence of viral RNA in non-ciliated cells of the human adenoid infected in vitro suggests that other cell types may also support rhinovirus replication.
authors.affiliation: Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville 22908.
authors.forename: E
authors.initials: E
authors.lastname: de Arruda
authors.forename: T E
authors.initials: TE
authors.lastname: Mifflin
authors.forename: J M
authors.initials: JM
authors.lastname: Gwaltney
authors.forename: B
authors.initials: B
authors.lastname: Winther
authors.forename: F G
authors.initials: FG
authors.lastname: Hayden
mesh_terms: D000234:Adenoids / Q000382:microbiology; D000328:Adult; D001483:Base Sequence; D002478:Cells, Cultured; D006367:HeLa Cells; D006651:Histocytochemistry; D006801:Humans; D008969:Molecular Sequence Data; D009298:Nasal Polyps / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010850:Picornaviridae Infections / Q000382:microbiology; D015347:RNA Probes; D012367:RNA, Viral / Q000032:analysis; D012229:Rhinovirus / Q000235:genetics / Q000254:growth & development*
chemical_list: D015345:Oligonucleotide Probes; D015347:RNA Probes; D012367:RNA, Viral