Phenotypic characterization of antigenic hybrids of poliovirus.
Three poliovirus hybrids, modified in neutralization antigenic sites (NAgs) I or II, were characterized for several phenotypic traits. The modifications to the capsid interfered with some stage of the life-cycle of the virus, since all three hybrids were growth-impaired in comparison to poliovirus type 1 (Mahoney) [PV1 (M)], the wild-type parent virus. All hybrids exhibited a reduced growth rate and a small-plaque phenotype, but they were not temperature sensitive. Furthermore, only one hybrid was slightly less stable to heating than the parent virus; the other two were as stable as the parent. Therefore, decreased thermal stability of the capsid is not an important cause of the poor growth characteristics of these hybrids.
Murdin
A D
AD
Department of Microbiology, State University of New York, Stony Brook 11794.
Kameda
A
A
Murray
M G
MG
Wimmer
E
E
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D002213:Capsid / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D002678:Chimera; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010641:Phenotype; D017955:Poliovirus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D013696:Temperature; D010948:Viral Plaque Assay; D014779:Virus Replication
D000956:Antigens, Viral