Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs.
A lambda gt11 cDNA library constructed from human tracheo-bronchial mucosa was screened with a polyclonal antiserum raised to chemically deglycosylated pronase glycopeptides from human bronchial mucins. Out of 20 positives clones, one partial cDNA clone was isolated and allowed to map a novel human tracheo-bronchial mucin gene. It contains 48 nucleotide tandem repeats quite perfectly identical which encodes a protein containing about 50% of hydroxy amino-acids. This clone hybridized to polydisperse messages produced by human tracheo-bronchial and human colonic mucosae. The gene (proposed name MUC 4) from which cDNA is derived maps to chromosome 3.
Porchet
N
N
Unité 16 INSERM, Lille, France.
Nguyen
V C
VC
Dufosse
J
J
Audie
J P
JP
Guyonnet-Duperat
V
V
Gross
M S
MS
Denis
C
C
Degand
P
P
Bernheim
A
A
Aubert
J P
JP
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001980:Bronchi / Q000502:physiology; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000235:genetics; D014132:Trachea / Q000502:physiology
D009077:Mucins; D012333:RNA, Messenger; D004247:DNA