title: Modulation of GABA-gated chloride currents by intracellular Ca2+ in cultured porcine melanotrophs.
abstract: 1. The modulatory role of intracellular Ca2+ concentration ([Ca2+]i) on gamma-aminobutyric acid type A (GABAA) receptor-gated Cl- currents was investigated in dialysed and intact cells of cultured porcine pituitary intermediate lobe (IL) cells using the patch-clamp technique. In order to isolate Ca2+ and Cl- currents all other membrane currents were blocked pharmacologically. Isoguvacine, a specific GABAA receptor agonist, was used to activate selectively GABAA receptor-mediated whole-cell and single-channel Cl- currents. 2. In the whole-cell recording (WCR) configuration inward Ca2+ currents triggered before and/or during the application of isoguvacine (100 microM), did not inhibit the GABAA receptor-mediated response. This lack of effect of calcium currents was obtained in all situations tested, i.e. when the intracellular Ca2+ concentration was only weakly buffered (0.5 mM-EGTA in the pipette solution), not buffered at all (no EGTA added to the pipette solution) or when the resting [Ca2+]i was buffered at 10(-7) M (pCa 7) with internal EGTA. 3. At pCa 7, simultaneous application of isoguvacine (100 microM) and caffeine (10 mM) resulted in a 47 +/- 15% reduction of the whole-cell GABAA response. In the same conditions, a ten times lower concentration of caffeine (1 mM), induced a transient increase of the GABAA response which turned into a steady-state inhibition during the subsequent applications. 4. At pCa 7, when isoguvacine (100 microM) was applied together with 3Me-His2-TRH (50 nM), a potent analogue of the calcium-recruiting thyrotrophin-releasing hormone, the GABAA receptor-gated Cl- current was increased by 40 +/- 8%. In the absence of the Ca2+ chelator EGTA in the pipette solution, either potentiating or inhibitory effects of 3Me-His2-TRH on the GABAA response were observed. 5. If a high concentration (18 mM) of the calcium chelator EGTA was included in the pipette solution, caffeine and 3Me-His2-TRH had markedly lower effects on the GABAA response than those observed at pCa 7, suggesting that the effect of both substances was mediated by an increase in [Ca2+]i. 6. In the absence of extracellular Ca2+, the effects of caffeine and 3Me-His2-TRH were not significantly different from those obtained in the presence of Ca2+ (5 mM), suggesting that Ca2+ influx was not the major route for increasing [Ca2+]i. 7. In the cell-attached (CA) configuration, the presence of isoguvacine (3-5 microM) in the pipette solution triggered the opening of channels displaying multiple current levels.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Institut de Physiologie (URA 309 CNRS), Université Louis Pasteur, Strasbourg, France.
authors.forename: D
authors.initials: D
authors.lastname: Mouginot
authors.forename: P
authors.initials: P
authors.lastname: Feltz
authors.forename: R
authors.initials: R
authors.lastname: Schlichter
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism*; D004594:Electrophysiology; D005260:Female; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D010902:Pituitary Gland / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D013552:Swine; D013973:Thyrotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D015220:Calcium Channels; D002712:Chlorides; C014109:thyrotropin-releasing hormone, 3-Me-; D002110:Caffeine; D005680:gamma-Aminobutyric Acid; D013973:Thyrotropin-Releasing Hormone; D002118:Calcium; D011761:Pyrrolidonecarboxylic Acid