title: Energy requirements for protein translocation across the Escherichia coli inner membrane.
abstract: Both ATP and an electrochemical potential play roles in translocating proteins across the inner membrane of Escherichia coli. Recent discoveries have dissected the overall transmembrane movement into separate subreactions with different energy requirements, identified a translocation ATPase, and reconstituted both energy-requiring steps of the reaction from purified components. A more refined understanding of the energetics of this fundamental process is beginning to provide answers about the basic issues of how proteins move across the hydrophobic membrane barrier.
authors.affiliation: Department of Microbiology, Oregon State University, Corvallis 97331-3804.
authors.forename: B L
authors.initials: BL
authors.lastname: Geller
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry*; D001693:Biological Transport, Active / Q000502:physiology; D002462:Cell Membrane / Q000201:enzymology*; D004734:Energy Metabolism / Q000502:physiology*; D004926:Escherichia coli / Q000201:enzymology*; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000737:chemistry; D008954:Models, Biological; D011506:Proteins / Q000378:metabolism*; D011522:Protons
chemical_list: D008565:Membrane Proteins; D011506:Proteins; D011522:Protons; D000251:Adenosine Triphosphatases