A three-step purification of manganese superoxide dismutase from human liver on both large and small scales.
A new method for the purification of manganese superoxide dismutase from human liver is described. The procedure involves essentially three steps: DEAE-cellulose, hydroxylapatite, and butyl-Toyopearl chromatographies. The method has several advantages: (i) its simplicity and rapidity (it takes less than 3 days), (ii) its high yield (62%) with a high specific activity (5660 units/mg of purified SOD), and (iii) its suitability for both large- and small-scale purifications.
Matsuda
Y
Y
Department of Biochemistry, Osaka University Medical School, Suita, Japan.
Suzuki
K
K
Ookawara
T
T
Nakata
T
T
Seo
H G
HG
Kawata
S
S
Tarui
S
S
Deutsch
H F
HF
Taniguchi
N
N
D002845:Chromatography / Q000379:methods*; D002848:Chromatography, DEAE-Cellulose; D017886:Durapatite; D006801:Humans; D006882:Hydroxyapatites; D008099:Liver / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000302:isolation & purification*
D006882:Hydroxyapatites; D017886:Durapatite; D013482:Superoxide Dismutase