title: Reactivity of the Bacillus subtilis succinate dehydrogenase complex with quinones.
abstract: The succinate dehydrogenase isolated from Bacillus subtilis was found to catalyze the oxidation of succinate with hydrophilic quinones. Either naphthoquinones or benzoquinones served as acceptors. The enzyme activity increased with the redox potential of the quinone. The highest turnover number was commensurate with that of the bacterial succinate respiration in vivo. The succinate dehydrogenase was similarly active in fumarate reduction with quinols. The highest activity was obtained with the most electronegative quinol. The fumarate reductase isolated from Wolinella succinogenes catalyzed succinate oxidation with quinones and fumarate reduction with the corresponding quinols at activities similar to those of the B. subtilis enzyme. Succinate oxidation by the lipophilic quinones, ubiquinone or vitamin K-1, was monitored as cytochrome c reduction using proteoliposomes containing succinate dehydrogenase together with the cytochrome bc1 complex. The activity with ubiquinone or vitamin K-1 was commensurate with the succinate respiratory activity of bacteria or of the bacterial membrane fraction. The results suggest that menaquinone is involved in the succinate respiration of B. subtilis, although its redox potential is unfavorable.
authors.affiliation: Institut für Mikrobiologie, J.W. Goethe-Universität, Frankfurt am Main, F.R.G.
authors.forename: E
authors.initials: E
authors.lastname: Lemma
authors.forename: C
authors.initials: C
authors.lastname: Hägerhäll
authors.forename: V
authors.initials: V
authors.lastname: Geisler
authors.forename: U
authors.initials: U
authors.lastname: Brandt
authors.forename: G
authors.initials: G
authors.lastname: von Jagow
authors.forename: A
authors.initials: A
authors.lastname: Kröger
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D003574:Cytochrome c Group / Q000378:metabolism; D050356:Lipid Metabolism; D010084:Oxidation-Reduction; D011809:Quinones / Q000378:metabolism*; D012995:Solubility; D013385:Succinate Dehydrogenase / Q000378:metabolism*
chemical_list: D003574:Cytochrome c Group; D011809:Quinones; D013385:Succinate Dehydrogenase