title: Regulation of guanine nucleotide binding regulatory proteins in cultured adrenal cells by adrenocorticotropin and angiotensin-II.
abstract: In addition to their steroidogenic effect on cultured bovine adrenal fasciculata cells ACTH and angiotensin-II (A-II) have a long term effect on the ability of these cells to respond to subsequent hormonal stimulation. The present work explores the effects of a 72-h pretreatment of adrenal cells with both hormones on the first steps of the mechanism of action of ACTH and A-II and on the amounts of the alpha-subunits of guanine nucleotide binding proteins Gs and Gi. ACTH but not A-II increased acute ACTH or cholera toxin-induced cAMP production. Moreover, ACTH but not A-II enhanced the amount of alpha S protein evaluated by cholera toxin ADP ribosylation, whereas both hormones elevated immunoblotted alpha S. Both hormones increased A-II induced phosphoinositide breakdown and Ca2+ uptake without modification of the A-II potentiating effect on ACTH-induced cAMP production. Treatment of cells with pertussis toxin (PT, 0.5 micrograms/ml) for the last 24 h reduced by 27% the A-II induced phosphoinositide breakdown in A-II pretreated cells but had no significant effect in ACTH-pretreated cells. No effect of PT was observed on A-II induced Ca2+ uptake or on its potentiating action on ACTH-induced cAMP production in ACTH as well as A-II-pretreated cells. Moreover, both hormones increased Gi proteins (40-41 kDa) evaluated by PT ADP ribosylation. Immunoblot analysis revealed that ACTH preferentially enhanced alpha i3, whereas the stimulatory effect of A-II was more marked on alpha i1 and alpha i2. These results indicate that in bovine adrenal fasciculata cells, peptide hormones settle target cell responsiveness not only by regulating the membrane-bound receptors, but also by modulating the level of G proteins coupling these receptors to the intracellular signals.
authors.affiliation: INSERM U 307, Hôpital Debrousse, Lyon, France.
authors.forename: M
authors.initials: M
authors.lastname: Bégeot
authors.forename: D
authors.initials: D
authors.lastname: Langlois
authors.forename: A M
authors.initials: AM
authors.lastname: Spiegel
authors.forename: J M
authors.initials: JM
authors.lastname: Saez
mesh_terms: D000311:Adrenal Glands / Q000166:cytology / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001345:Autoradiography; D002118:Calcium / Q000493:pharmacokinetics; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D019204:GTP-Binding Proteins / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D013997:Time Factors
chemical_list: D010716:Phosphatidylinositols; D000804:Angiotensin II; D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D002118:Calcium