title: Stretch increases inositol trisphosphate and inositol tetrakisphosphate in cultured pulmonary vascular smooth muscle cells.
abstract: There are no reports of the effect of stretch on inositol phosphates in smooth muscle. Phosphoinositide and inositol phosphate metabolism was studied in cultured rat vascular smooth muscle cells subjected to stretching. The masses of inositol trisphosphate and tetrakisphosphate increased (+34 +/- 7% and +58 +/- 12%, respectively; p less than 0.001) after 25 s of a single 20% stretch and had returned to control levels by 45 s; phosphatidylinositol, phosphatidylinositol phosphate and bisphosphate did not change. Repetitive stretch did not alter the masses of any of the compounds. A single stretch also increased 45Ca2+ efflux (+52 +/- 5%, p less than 0.01). These data suggest that stretch of cultured vascular smooth muscle can elicit a rapid, short-lived increase in inositol phosphates, which may subsequently affect Ca2+.
authors.affiliation: Department of Cardiology, Children's Hospital (Boston), MA 02115.
authors.forename: T J
authors.initials: TJ
authors.lastname: Kulik
authors.forename: R A
authors.initials: RA
authors.lastname: Bialecki
authors.forename: W S
authors.initials: WS
authors.lastname: Colucci
authors.forename: A
authors.initials: A
authors.lastname: Rothman
authors.forename: E T
authors.initials: ET
authors.lastname: Glennon
authors.forename: R H
authors.initials: RH
authors.lastname: Underwood
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007295:Inositol Phosphates / Q000378:metabolism*; D007700:Kinetics; D009126:Muscle Relaxation; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000502:physiology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D011651:Pulmonary Artery / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium