Gene rearrangements detected by nonradioactive digoxigenin-labeled DNA probes.
The configurations of immunoglobulin, T-cell receptor beta chain and bcl-2 genes were analyzed in lympho-proliferative disorders using nonradioactive digoxigenin-labeled probes. The studies demonstrated the reliability of digoxigenin-labeled probes for the detection of single-copy genes after Southern blotting of genomic DNA: 1 microgram and sometimes even 0.2 microgram of restriction endonuclease-digested DNA could be detected either by JH, C beta or bcl-2 probes. The intensity of the signal was consistently satisfactory, and there was no background problem. Control experiments showed neither false-positive nor false-negative results caused by the use of the nonradioactive detection system.
Ptaszynski
K
K
Department of Genetics and Pathology, Medical Academy, Szczecin, Poland.
Podolski
J
J
Huebner
K
K
Lubinski
J
J
D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004076:Digoxigenin; D015321:Gene Rearrangement / Q000235:genetics*; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000235:genetics; D005803:Genes, Immunoglobulin / Q000235:genetics; D006801:Humans; D007144:Immunoglobulin J-Chains / Q000235:genetics; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000235:genetics / Q000276:immunology; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics
D015342:DNA Probes; D007144:Immunoglobulin J-Chains; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA; D004076:Digoxigenin