Ciprofloxacin resistance in coagulase-positive and -negative staphylococci: role of mutations at serine 84 in the DNA gyrase A protein of Staphylococcus aureus and Staphylococcus epidermidis.
gyrA mutations in quinolone-resistant pathogenic isolates of Staphylococcus spp. have been detected by the direct HinfI digestion of polymerase chain reaction products. Homology among gyrA genes allowed rapid examination of both coagulase-positive and -negative isolates. DNA sequence analysis revealed that ciprofloxacin resistance in Staphylococcus epidermidis is associated with a novel Ser-84----Phe mutation in the DNA gyrase A protein, analogous to Ser-84----Leu changes observed in Staphylococcus aureus.
Sreedharan
S
S
Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, United Kingdom.
Peterson
L R
LR
Fisher
L M
LM
D000890:Anti-Infective Agents / Q000494:pharmacology; D001483:Base Sequence; D002939:Ciprofloxacin / Q000494:pharmacology*; D003030:Coagulase / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004352:Drug Resistance, Microbial; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012694:Serine / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics
D000890:Anti-Infective Agents; D003030:Coagulase; D012694:Serine; D002939:Ciprofloxacin; D004250:DNA Topoisomerases, Type II