title: Intracellular pH and sodium-proton exchange activity of lymphocytes in stroke-prone spontaneously hypertensive rats.
abstract: 1. At the age of 20 weeks, intracellular pH (pHi) of circulating lymphocytes suspended in HCO(3-)-free NaCl media was not significantly different between stroke-prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar-Kyoto rats (WKY). 2. The initial recovery rate of pHi in lymphocytes tended to be greater in SHRSP than in WKY after the addition of 60 mmol/L or 120 mmol/L of NaCl, but there was no statistically significant difference. 3. The H+ equivalent efflux rate, which was a true reflection of Na(+)-H(+) activity, was significantly greater in SHRSP than in WKY (P less than 0.05). The difference in H+ equivalent efflux rate was not due to the difference in cellular buffering power between the two groups (P greater than 0.05). An increased Na(+)-H(+) exchange activity may play a partial role in the pathogenesis of hypertension.
authors.affiliation: Department of Pediatrics, Shimane Medical University, Izumo, Japan.
authors.forename: D Y
authors.initials: DY
authors.lastname: Li
authors.forename: A
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Nara
authors.forename: K
authors.initials: K
authors.lastname: Ikeda
authors.forename: C
authors.initials: C
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Yamori
mesh_terms: D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000502:physiology*; D002561:Cerebrovascular Disorders / Q000097:blood / Q000503:physiopathology; D005452:Fluoresceins; D006863:Hydrogen-Ion Concentration; D006973:Hypertension / Q000097:blood / Q000503:physiopathology*; D007424:Intracellular Fluid / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012964:Sodium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers
chemical_list: D002352:Carrier Proteins; D005452:Fluoresceins; D017923:Sodium-Hydrogen Exchangers; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D012964:Sodium