Parallel modulation of brown adipose tissue GDP-binding, substrate uptake and (Na(+)-K+)-ATPase activity in the rat.
Brown adipose tissue (Na(+)-K+)-ATPase activity, in vitro glucose uptake and 2-aminoisobutyric acid uptake, as well as mitochondrial GDP-binding and succinate dehydrogenase activity were determined in order to study the relationship between these parameters in control, cold acclimated and cafeteria-fed rats. GDP-binding, (Na(+)-K+)-ATPase and glucose uptake were increased in interscapular brown adipose tissue from cold-acclimated and cafeteria-fed rats, whereas 2-aminoisobutyric acid uptake was only increased in cafeteria-fed rats. GDP-binding and (Na(+)-K+)-ATPase activity showed a high correlation coefficient suggesting a parallel modulation of both systems, which would probably share a common regulation mechanism.
Zamora
F
F
Department d'Enginyeria Química i Bioquímica, Facultat de Ciències Químiques de Tarragona, Universitat de Barcelona, Tarragona, Spain.
Alemany
M
M
Arola
L
L
D002001:Adipose Tissue, Brown / Q000201:enzymology / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D001833:Body Temperature Regulation; D001835:Body Weight; D003080:Cold Temperature; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism
D000621:Aminoisobutyric Acids; D007328:Insulin; D006153:Guanosine Diphosphate; C100049:2-aminoisobutyric acid; D013385:Succinate Dehydrogenase; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose