Unsaturated phosphatidylcholines inhibit superoxide production in human neutrophils.
Unsaturated long chain phosphatidylcholines such as phosphatidylcholine dioleoyl and phosphatidylcholine dilinoleoyl in micromolar concentrations inhibited the superoxide production in neutrophils stimulated by the activators of protein kinase C, phorbol 12-myristate 13-acetate and 1,2-dioctanoyl-sn- glycerol. In contrast, the superoxide production induced by surface receptor agonist, formyl-methionyl-leucyl-phenylalanine, was unaffected by the phospholipids. These data suggest that surfactant phosphatidylcholines may have a modulatory role on neutrophil oxidative burst in the lung during inflammation where there is a preponderance of unsaturated phosphatidylcholines.
Yoshida
K
K
John B. Pierce Laboratory, Yale University School of Medicine, New Haven, Connecticut 06519.
Mohsenin
V
V
D000704:Analysis of Variance; D002458:Cell Fractionation; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004075:Diglycerides; D010713:Phosphatidylcholines; C044295:1,2-dioctanoylglycerol; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D011494:Protein Kinases; D013755:Tetradecanoylphorbol Acetate