Restriction endonuclease analysis as a taxonomic tool in the study of pig isolates belonging to the Australis serogroup of Leptospira interrogans.
Restriction endonuclease analysis was performed on DNAs from the type strains of the Australis serogroup of Leptospira interrogans by using 20 restriction enzymes, and the electrophoretic patterns obtained were compared with patterns obtained from 162 Australis serogroup isolates from pigs. It proved to be a quick and reliable method for typing such strains. All of the pig isolates were identified as either serovar bratislava or muenchen. It also showed differences at the subserovar level which may be important in (i) understanding the epidemiology of the Australis serogroup, (ii) the development of suitable vaccines, and (iii) pathogenesis and pathogenicity studies. Two genotypes (B2b and M2) accounted for 92% of isolates from aborted or stillborn piglets, while a third genotype (B2a) was the only one recovered from the brains of piglets with meningitis.
Ellis
W A
WA
Veterinary Research Laboratories, Belfast, Northern Ireland, United Kingdom.
Montgomery
J M
JM
Thiermann
A B
AB
D000818:Animals; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D007921:Leptospira interrogans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012703:Serotyping; D013045:Species Specificity; D013552:Swine / Q000382:microbiology
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes