[The synthetic peptide 62-75 VP3 of the hepatitis A virus induces virus-binding antibodies].
Peptide (P6) representing amino acids 62-75 of HAV capsid protein VP3 was synthesized. Pure P6, octamer form of P6, and P6 conjugated to KLH were injected into rabbits. The sera against these preparations reacted with denaturized VP3 in immunoblotting tests; however, only anti-P6-KLH serum was capable of binding the native HAV.
Kusov
Iu Iu
IuIu
Kazachkov
Iu A
IuA
Dzagurov
G K
GK
Nastashenko
T A
TA
Kozhich
A T
AT
Gabrielian
A E
AE
Chikin
L D
LD
Ivanov
V T
VT
Balaian
M S
MS
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D001666:Binding Sites, Antibody / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D006507:Hepatovirus / Q000276:immunology*; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization / Q000379:methods; D015151:Immunoblotting; D016056:Immunodominant Epitopes / Q000276:immunology; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D014764:Viral Proteins / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D007106:Immune Sera; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D010455:Peptides; D014764:Viral Proteins; C070597:hepatitis A virus, VP3 protein (62-75)
Sinteticheskiĭ peptid 62-75 VP3 virusa gepatitia A indutsiruet virussviazyvaiushchie antitela.