[Monoclonal antibodies to Rho(D)-antigen of the Rhesus system. Obtaining the EBV-transformed B-lymphoblastoid human cell lines secreting IgG-anti-D antibodies].
Human B-lymphoblastoid cell lines producing specific anti-D antibodies against Rh0(D) antigen have been established by EBV-transformation. The strong influence of the donor's immune status (serum titer of anti-D antibodies, intravenous booster injection prior to bleeding) is shown. The cell lines cloned by limiting dilution continuously produce anti-D antibodies in culture for 7 months or longer which is sufficient to support large-scale production of anti-D antibodies based on the cultivation of cryopreserved cells. Anti-D IgGl monoclonal antibodies obtained react with Rh-positive and Du cells but not with Rh-negative cells in serological tests. These human monoclonal antibodies could be useful in the prevention of Rh hemolytic disease of newborns.
Deriugina
E I
EI
Olovnikova
N I
NI
Lemeneva
L N
LN
Belkina
E V
EV
Udalov
G A
GA
Chertkov
I L
IL
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001709:Biotechnology / Q000379:methods*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000276:immunology*; D002478:Cells, Cultured; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D066298:In Vitro Techniques; D007518:Isoantibodies / Q000096:biosynthesis* / Q000276:immunology; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System
Monoklonal'nye antitela k Rho(D)-antigenu sistemy Rezus. Poluchenie EBV-transformirovannykh B-limfoblastoidnykh kletochnykh linii cheloveka, sekretiruiushchikh IgG-anti-D antitela.