title: Inhibition of the degranulation and myeloperoxidase activity of human polymorphonuclear neutrophils by Brucella melitensis.
abstract: The degranulation and myeloperoxidase-H2O2-halide activities of human polymorphonuclear leukocytes from healthy donors were tested after co-incubation with either Brucella melitensis 16M, Staphylococcus aureus or Staphylococcus aureus in presence of lipopolysaccharide, protein fraction, native hapten and soluble fractions released at 65 degrees C from smooth strain of Brucella melitensis 16M. The degranulation and myeloperoxidase activities of polymorphonuclear leukocytes were significantly higher when co-incubated with Staphylococcus aureus than with Brucella melitensis. The presence of lipopolysaccharide, protein fraction, and native hapten did not cause significant modification of either degranulation or myeloperoxidase activities of polymorphonuclear leukocytes against Staphylococcus aureus. Soluble fraction released at 65 degrees C produced a significant reduction in the myeloperoxidase activity but did not alter the degranulation of polymorphonuclear leukocytes triggered by Staphylococcus aureus.
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Valladolid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Orduña
authors.forename: C
authors.initials: C
authors.lastname: Orduña
authors.forename: J M
authors.initials: JM
authors.lastname: Eiros
authors.forename: M A
authors.initials: MA
authors.lastname: Bratos
authors.forename: P
authors.initials: P
authors.lastname: Gutiérrez
authors.forename: P
authors.initials: P
authors.lastname: Alonso
authors.forename: A
authors.initials: A
authors.lastname: Rodríguez Torres
mesh_terms: D002002:Brucella / Q000276:immunology / Q000378:metabolism* / Q000472:pathogenicity; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000378:metabolism*; D005966:Glucuronidase / Q000032:analysis; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D009895:Opsonin Proteins; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology; D013211:Staphylococcus aureus / Q000276:immunology / Q000378:metabolism
chemical_list: D009895:Opsonin Proteins; D009195:Peroxidase; D005966:Glucuronidase