Vanadyl causes hydroxyl radical mediated degradation of deoxyribose.
Vanadyl caused a time- and dose-dependent degradation of deoxyribose to carbonyl products detectable with thiobarbituric acid. This process was inhibited by catalase, ethanol or HEPES; whereas superoxide dismutase was without effect. Vanadate did not substitute for vanadyl even in the presence of a source of O2- plus H2O2; but it did so in the presence of reductants such as thiols or NADH. It appears that hydrogen peroxide, generated by the autoxidation of vanadyl, is reduced by vanadyl to the hydroxyl radical; which, in turn, was responsible for the degradation of deoxyribose. A similar process might contribute to the toxic and pharmacological effects of vanadium salts.
Liochev
S
S
Institute of Physiology, Bulgarian Academy of Sciences, Sofia.
Ivancheva
E
E
D002374:Catalase / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D003855:Deoxyribose / Q000737:chemistry*; D004229:Dithiothreitol / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D006531:HEPES / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D006878:Hydroxides; D017665:Hydroxyl Radical; D007501:Iron / Q000494:pharmacology; D009243:NAD / Q000494:pharmacology; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D013482:Superoxide Dismutase / Q000494:pharmacology; D014638:Vanadates / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D006878:Hydroxides; D009243:NAD; D017665:Hydroxyl Radical; D000431:Ethanol; D014638:Vanadates; D003855:Deoxyribose; D006861:Hydrogen Peroxide; D007501:Iron; D002374:Catalase; D013482:Superoxide Dismutase; D003545:Cysteine; D006531:HEPES; D010100:Oxygen; D004229:Dithiothreitol