Activation of the cellular transcription factor AP-1 in herpes simplex virus infected cells is dependent on the viral immediate-early protein ICPO.
Lytic infection with herpes simplex virus (HSV) results in the repression of most host cell protein synthesis but produces an increased activity of the cellular AP-1 transcription factor. This increase is paralleled by an increase in the transcription rate of the proto-oncogene encoding the AP-1 component, c-Jun resulting in an increase in c-Jun protein in infected cells. The increased AP-1 activity in infected cells is dependent upon the HSV immediate-early protein ICPO. Thus a mutant lacking the gene encoding this protein fails to increase AP-1 activity whilst an ICPO expression plasmid can specifically increase the activity of an AP-1 dependent promoter in co-transfection experiments. The implications of these effects in the interaction of HSV with cultured cells are discussed.
Jang
K L
KL
Department of Biochemistry, University College and Middlesex School of Medicine, London, UK.
Pulverer
B
B
Woodgett
J R
JR
Latchman
D S
DS
D001665:Binding Sites; D015153:Blotting, Western; D002460:Cell Line; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D017874:Immediate-Early Proteins; D009154:Mutation; D010957:Plasmids; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D018139:Simplexvirus / Q000502:physiology*; D014162:Transfection; D044767:Ubiquitin-Protein Ligases; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
D017874:Immediate-Early Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun; D014764:Viral Proteins; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1