Inhibition of cell growth and tumorigenesis of human glioblastoma cells by a neutralizing antibody against human basic fibroblast growth factor.
We report here that a neutralizing mouse monoclonal antibody against basic FGF inhibited both anchorage-dependent and anchorage-independent growth of U-87MG and T98G human glioblastoma cells and HeLa cells, all of which express both the basic FGF and the FGF receptor genes. In addition, the subcutaneous administration of this antibody significantly suppressed the tumor development of these tumor cells in nude mice. Therefore, basic FGF plays an important role in neoplastic growth of these cells. The neutralization of basic FGF will be effective in controlling the growth of tumors, such as glioblastoma and other cancer cells which bear basic FGF and FGF receptors.
Takahashi
J A
JA
Department of Molecular Virology, Kyoto University, Japan.
Fukumoto
M
M
Kozai
Y
Y
Ito
N
N
Oda
Y
Y
Kikuchi
H
H
Hatanaka
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015152:Blotting, Northern; D002455:Cell Division; D016222:Fibroblast Growth Factor 2 / Q000276:immunology* / Q000502:physiology; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000473:pathology*; D006367:HeLa Cells; D006801:Humans; D007279:Injections, Subcutaneous; D007700:Kinetics; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011956:Receptors, Cell Surface / Q000032:analysis; D017468:Receptors, Fibroblast Growth Factor; D014410:Tumor Stem Cell Assay
D000911:Antibodies, Monoclonal; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2