title: Parathyroid hormone enhances gentamicin uptake by opossum kidney cells but not by LLC-PK1 cells.
abstract: Parathyroidectomy is proposed to be protective against gentamicin nephrotoxicity in rats. In the present study, we evaluated the effects of bovine PTH(1-34) on gentamicin accumulation in cultured kidney epithelial cell lines, opossum kidney (OK) cells which possess PTH receptors and LLC-PK1 cells which are devoid of PTH receptors. Ten days after seeding, the culture medium was exchanged for medium containing 1 mM gentamicin and bovine PTH. The cell gentamicin concentration was measured by a substrate-labeled fluorescence immunoassay (TDA gentamicin kit). Gentamicin uptake was accelerated by bovine PTH in OK cells but not in LLC-PK1 cells. The enhancing effect of bovine PTH seems to be mediated by a cAMP-dependent process. The results suggest that PTH accelerates gentamicin accumulation in renal tissues and potentiates gentamicin nephrotoxicity.
authors.affiliation: First Department of Medicine, Osaka University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakahama
authors.forename: M
authors.initials: M
authors.lastname: Kakihara
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuhara
authors.forename: N
authors.initials: N
authors.lastname: Ueda
authors.forename: Y
authors.initials: Y
authors.lastname: Orita
authors.forename: T
authors.initials: T
authors.lastname: Kamada
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D005839:Gentamicins / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009893:Opossums; D010281:Parathyroid Hormone / Q000502:physiology*; D011506:Proteins / Q000378:metabolism; D013552:Swine
chemical_list: D005839:Gentamicins; D010281:Parathyroid Hormone; D011506:Proteins; D003994:Bucladesine; D000242:Cyclic AMP