title: Restriction endonuclease patterns of herpes simplex virus DNA: subtyping of HSV-1 and HSV-2 strains from genital and nongenital lesions.
abstract: Herpes simplex type 1 (HSV-1) and type 2 (HSV-2) isolates from genital and nongenital infections were submitted to restriction endonuclease analysis for possible genomic changes in relation with the adaptation of the virus to a new site on the body. HSV-1 and HSV-2 strains were successfully divided into two subgroups using the Hin c II restriction enzyme. No correlation was found, however, between the proposed genomic subtypes H1A, H1B, H2A and H2B, and the genital or nongenital origin of the HSV strains.
authors.affiliation: Centre de Recherche en Virologie, Institut Armand-Frappier, Laval-des-Rapides, Québec, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Hamelin
authors.forename: A
authors.initials: A
authors.lastname: Pramatarova
authors.forename: J
authors.initials: J
authors.lastname: Portnoy
authors.forename: M
authors.initials: M
authors.lastname: Gerson
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D006558:Herpes Genitalis / Q000382:microbiology*; D006561:Herpes Simplex / Q000382:microbiology*; D006801:Humans; D008297:Male; D018139:Simplexvirus / Q000145:classification / Q000235:genetics*; D014709:Vero Cells
chemical_list: D004279:DNA, Viral; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C024400:GTYRAC-specific type II deoxyribonucleases