The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells.
Wilms' tumor, a pediatric nephroblastoma, has been associated with genetic alterations of the 11p13 and 11p15 regions. The introduction of a der(11) chromosome into the G401 Wilms' tumor cell line has been shown previously to revert the tumorigenic phenotype. A subtractive cDNA/RNA hybridization performed between the tumorigenic parent (G401) and a nontumorigenic microcell hybrid of G401 (110.1/G401.1) containing the der(11) chromosome resulted in the identification of a single novel cDNA clone, designated QM. The cDNA is 745 nucleotides in length and encodes a predicted hydrophilic 25 kd basic protein, primarily consisting of alpha helices. The QM transcript is expressed in a wide variety of embryonic and adult tissues and demonstrates a down regulation of expression in adult kidney and heart. QM is also a member of a multigene family members of which map to chromosomes 6 and 14. The QM mRNA level is modulated between the tumorigenic and nontumorigenic cell lines and therefore may be involved in the maintenance of the nontumorigenic phenotype.
Dowdy
S F
SF
Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine 92717.
Lai
K M
KM
Weissman
B E
BE
Matsui
Y
Y
Hogan
B L
BL
Stanbridge
E J
EJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015139:Blotting, Southern; D002460:Cell Line; D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D016147:Genes, Tumor Suppressor; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D009396:Wilms Tumor / Q000235:genetics*
D004273:DNA, Neoplasm; D012333:RNA, Messenger