High-performance liquid chromatographic (HPLC) analysis of oligomeric components of the spermicide nonoxynol-9.
The commercially available Nonoxynol-9 spermicide is a multicomponent mixture of oligomers. When Nonoxynol-9 was separated by normal phase gradient HPLC, 17 components were shown to exist in the commercial mixture. These oligomeric components follow a Poisson distribution around the most abundant oligomer, EO 8 (11.7%). Select oligomers were isolated by preparative HPLC (Rt = 19.6, 34.0, 45.6, 51.2, 61.6, and 79.2 min) and purified by HPLC. These were identified by FAB-MS and NMR to be the oligomers EO 3, EO 6, EO 8, EO 9, EO 11, and EO 16, respectively.
Walter
B A
BA
College of Pharmacy, University of Kentucky, Lexington 40536-0082.
Digenis
G A
GA
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D017137:Nonoxynol; D011092:Polyethylene Glycols / Q000032:analysis / Q000302:isolation & purification*; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013089:Spermatocidal Agents / Q000032:analysis / Q000302:isolation & purification*
D013089:Spermatocidal Agents; D017137:Nonoxynol; D011092:Polyethylene Glycols