Evidence for a critical role of GABAergic transmission within the thalamus in the genesis and control of absence seizures in the rat.
The involvement of GABAergic transmission within the thalamus in the generation and control of spike and wave discharges (SWD) in generalized non-convulsive or absence epilepsy was studied in rats with spontaneous SWD and in non-epileptic rats. In epileptic rats, bilateral injections of gamma-vinyl GABA (GVG, 10 micrograms/side) or muscimol (10 ng/side) into the medial part of the ventral lateral thalamus, i.e. the specific relay nuclei, significantly increased spontaneous cortical SWD whereas similar injections into the most lateral part of the thalamus, i.e. the area of the reticular nuclei, significantly suppressed these seizures. Injections of GVG (20 micrograms) or muscimol (20 ng) into the midline thalamus had no direct effect on the spontaneous SWD. In non-epileptic rats, injections of GVG (25 micrograms/side) or muscimol (100 ng/side) into the thalamic relay nuclei produced short SWD on the cortical EEG. These results suggest that GABAergic neurons in the reticular nuclei and their projections to the specific relay nuclei of the thalamus are involved in the elicitation and control of generalized non-convulsive seizures.
Liu
Z
Z
Département de Neurophysiologie et Biologie des Comportements, Centre de Neurochimie du C.N.R.S., Strasbourg, France.
Vergnes
M
M
Depaulis
A
A
Marescaux
C
C
D000614:Aminocaproates / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D004195:Disease Models, Animal; D004569:Electroencephalography; D004832:Epilepsy, Absence / Q000503:physiopathology*; D005071:Evoked Potentials / Q000187:drug effects; D008297:Male; D008564:Membrane Potentials; D008845:Microinjections; D009118:Muscimol / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013238:Stereotaxic Techniques; D009435:Synaptic Transmission / Q000187:drug effects; D013788:Thalamus / Q000187:drug effects / Q000502:physiology / Q000503:physiopathology*; D020888:Vigabatrin; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D000614:Aminocaproates; D000927:Anticonvulsants; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D020888:Vigabatrin