The effect of somatostatin 201-995 on the early course of porcine pancreaticoduodenal allotransplantation.
This study was undertaken to determine the effects of somatostatin 201-995 (SMS) on the maintenance dose of intravenous cyclosporine and on graft blood flow, exocrine secretion, and rejection after porcine pancreaticoduodenal allotransplantation (PDA). For seven days, 12 pigs (6 control, 6 SMS-treated) were studied to determine the effects of SMS on serum CsA concentrations. Twenty-six pigs (14 control, 12 SMS) with streptozocin-induced diabetes underwent PDA. Blood flow was measured through graft celiac and superior mesenteric arteries 15 and 60 min after reperfusion. SMS (75 micrograms s.c.) was given after the 15-min blood-flow measurement in the SMS group. Sixteen pigs (8 control, 8 SMS) were followed postoperatively with daily measurements of serum glucose and amylase concentrations, and urine amylase and trypsin activities. All pigs were immunosuppressed with azathioprine, prednisone, and i.v. CsA. SMS pigs also received SMS (75 micrograms s.c.) every 8 hr. SMS had no effect on maintenance dose of CsA or on serum amylase, urine amylase, or urine trypsin activities. Mean days to rejection were also not affected. Intraoperative graft blood flow was significantly decreased by SMS, but incidence of graft thrombosis was unchanged. These results suggest that in the porcine PDA model, SMS does not appear to inhibit exocrine secretion and potentially may adversely affect the early course of PDA by decreasing graft blood flow.
Nicholson
C P
CP
Department of Surgery, Mayo Clinic, Rochester, Minnesota 55905.
Barr
D
D
Oeltjen
M R
MR
Munn
S R
SR
DiMagno
E P
EP
Carpenter
H A
HA
Sarr
M G
MG
Perkins
J D
JD
D000818:Animals; D003524:Cyclosporins / Q000378:metabolism; D004386:Duodenum / Q000637:transplantation*; D006649:Histocompatibility Antigens / Q000032:analysis; D010179:Pancreas / Q000098:blood supply / Q000187:drug effects; D016035:Pancreas Transplantation; D012039:Regional Blood Flow / Q000187:drug effects; D013004:Somatostatin / Q000494:pharmacology* / Q000633:toxicity; D013552:Swine; D014184:Transplantation, Homologous
D003524:Cyclosporins; D006649:Histocompatibility Antigens; D013004:Somatostatin