title: Expression of lipoprotein lipase mRNA in rat heart is localized mainly to mesenchymal cells as studied by in situ hybridization.
abstract: The expression of lipoprotein lipase mRNA (LPL mRNA) was studied in rat hearts by use of a sulfur-35-labeled antisense mRNA probe. Rats were studied under three conditions: fed, fasted, and injected with cholera toxin (an irreversible agonist of adenylate cyclase) and then fasted. The highest LPL activity was found in the hearts of cholera toxin-injected, fasted rats. After injection of cholera toxin, LPL mRNA levels were 3.5-fold higher than those from fed rats. Using in situ hybridization, we studied the site of expression of LPL mRNA under the same three experimental conditions. In sections of hearts from cholera toxin-injected, fasted rats, concentrations of autoradiographic grains, representing the site of LPL mRNA, were seen over interstitial elements, which comprise capillary and perivascular cells. A more diffuse and sparse reaction was seen over cardiac myocytes and was not always distinguishable from background. A similar but much less definitive localization was seen in sections of hearts from fasted rats. The present results indicate that in the rat heart, the main site of LPL synthesis and processing, especially after stimulation with an irreversible agonist of adenylate cyclase, is localized to interstitial elements rather than to adult cardiac myocytes.
authors.affiliation: Department of Experimental Medicine and Cancer Research, Hebrew University Hadassah Medical, Jerusalem, Israel.
authors.forename: O
authors.initials: O
authors.lastname: Stein
authors.forename: Y
authors.initials: Y
authors.lastname: Stein
authors.forename: S P
authors.initials: SP
authors.lastname: Schwartz
authors.forename: A
authors.initials: A
authors.lastname: Reshef
authors.forename: T
authors.initials: T
authors.lastname: Chajek-Shaul
authors.forename: M
authors.initials: M
authors.lastname: Ben-Naim
authors.forename: G
authors.initials: G
authors.lastname: Friedman
authors.forename: E
authors.initials: E
authors.lastname: Leitersdorf
mesh_terms: D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D001345:Autoradiography; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000008:administration & dosage; D004587:Electrophoresis, Agar Gel; D005215:Fasting; D006531:HEPES / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D010529:Peritoneal Cavity / Q000166:cytology; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D015347:RNA Probes; D012333:RNA, Messenger; D003994:Bucladesine; D002772:Cholera Toxin; D008071:Lipoprotein Lipase; D006531:HEPES