title: Comparison of Southern blot hybridization and polymerase chain reaction methods for the detection of human papillomavirus DNA.
abstract: A methodologic study was performed to compare the polymerase chain reaction (PCR) and Southern blot hybridization, two commonly used testing strategies for the detection of human papillomavirus (HPV) infection. Three laboratories tested masked aliquots of exfoliated cervical cell specimens obtained from 120 women by cervicovaginal lavage. The study population included 32 women with condylomatous atypia or cervical intraepithelial neoplasia and 88 control women with no known history of cervical neoplasia. Two laboratories used PCR with different sets of consensus primers for HPV detection. The third laboratory used low-stringency Southern blot hybridization to identify all HPV types, followed by high-stringency Southern and/or dot blot hybridization to confirm specific HPV types. One of the PCR primer sets detected HPV types with a differential efficiency that was not predicted by analysis of DNA sequences or direct testing of HPV-containing plasmids. In contrast, the second PCR primer set was shown to be a much broader consensus system, detecting the same HPV types as Southern blotting, though requiring much less clinical specimen. Over 80% of women with cervical intraepithelial neoplasia or condylomatous atypia were found to be HPV infected both by Southern blotting and by the second PCR primer set. Among the control women, 11% were HPV positive by Southern blotting, while 31% were positive with the second set of primers. Most of the HPV infections found only by PCR were not due to HPV type 6, 11, 16, 18, 31, 33, or 45. These known HPV types were uncommon among normal women in the study population, even as determined by the PCR method.
authors.affiliation: Environmental Epidemiology Branch, National Cancer Institute, Bethesda, Maryland 20892.
authors.forename: M H
authors.initials: MH
authors.lastname: Schiffman
authors.forename: H M
authors.initials: HM
authors.lastname: Bauer
authors.forename: A T
authors.initials: AT
authors.lastname: Lorincz
authors.forename: M M
authors.initials: MM
authors.lastname: Manos
authors.forename: J C
authors.initials: JC
authors.lastname: Byrne
authors.forename: A G
authors.initials: AG
authors.lastname: Glass
authors.forename: D M
authors.initials: DM
authors.lastname: Cadell
authors.forename: P M
authors.initials: PM
authors.lastname: Howley
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods*; D002584:Cervix Uteri / Q000382:microbiology; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014412:Tumor Virus Infections / Q000175:diagnosis; D002583:Uterine Cervical Neoplasms / Q000382:microbiology
chemical_list: D004279:DNA, Viral