Nanoscale separations combined with tandem mass spectrometry.
High-efficiency separations of peptide mixtures, tryptic digest and other biological compounds have been achieved using nanoscale packed capillaries and capillary zone electrophoresis (CZE). The coaxial continuous-flow fast atom bombardment design is an excellent interface for coupling these separation techniques with mass spectrometry (MS). In addition, this interface is very useful for the acquisition of MS-MS data from compounds separated by nanoscale packed capillary liquid chromatography and CZE. Structurally informative daughter-ion spectra can be obtained at the low picomole to femtomole level.
Deterding
L J
LJ
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Moseley
M A
MA
Tomer
K B
KB
Jorgenson
J W
JW
D000595:Amino Acid Sequence; D003366:Cosyntropin / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D004586:Electrophoresis; D013007:Growth Hormone-Releasing Hormone / Q000378:metabolism; D013058:Mass Spectrometry; D008832:Microchemistry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*; D014357:Trypsin
D003574:Cytochrome c Group; D010446:Peptide Fragments; D003366:Cosyntropin; D013007:Growth Hormone-Releasing Hormone; D014357:Trypsin