Chloroamphetamine did not prevent the effects of chronic antidepressants on 5-hydroxytryptamine inhibition of forskolin-stimulated adenylate cyclase in rat hippocampus.
Administration of p-chloroamphetamine (PCA, 10 mg/kg i.p. on two occasions) to rats resulted in a severe depletion of [3H]paroxetine binding sites, a measure of presynaptic serotonergic terminals, in both cortex and hippocampus, but did not affect [3H]8-hydroxy-2-(di-n-propylamino)tetralin [( 3H]8-OH-DPAT) binding or 5-hydroxytryptamine (5-HT)-induced inhibition of forskolin-stimulated adenylate cyclase in hippocampal membranes. Administration of either imipramine (15 mg/kg i.p. for 2 weeks) or lithium (0.2% for 2 weeks) to PCA-treated rats did not affect [3H]8-OH-DPAT binding but reduced the degree of inhibition of forskolin-stimulated adenylate cyclase by 5-HT in hippocampal membranes. It is concluded that the effects of imipramine and Li+ on 5-HT1A receptor-mediated responses in the hippocampus are exerted postsynaptically, possibly at a level distal to the receptor.
Newman
M E
ME
Ya'acov Herzog Center for Brain and Psychiatry Research, Ezrath Nashim Hospital, Jerusalem, Israel.
Ben-Zeev
A
A
Lerer
B
B
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D001665:Binding Sites; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D007099:Imipramine / Q000494:pharmacology; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D008297:Male; D017374:Paroxetine; D010880:Piperidines / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*; D013569:Synapses / Q000187:drug effects / Q000378:metabolism; D013764:Tetrahydronaphthalenes / Q000378:metabolism; D010133:p-Chloroamphetamine / Q000494:pharmacology*
D000067956:Adenylyl Cyclase Inhibitors; D000928:Antidepressive Agents; D002712:Chlorides; D010880:Piperidines; D013764:Tetrahydronaphthalenes; D005576:Colforsin; D012701:Serotonin; D017374:Paroxetine; D010133:p-Chloroamphetamine; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D008094:Lithium; D000262:Adenylyl Cyclases; D018021:Lithium Chloride; D007099:Imipramine