Calcium channel in isolated outer hair cells of guinea pig cochlea.
The physiological and pharmacological properties of the Ca2+ channel in outer hair cells (OHCs) freshly isolated from guinea pig cochlea were investigated using a whole-cell patch-clamp technique. The Ca2+ current (ICa) was activated from a membrane potential of -20 mV and reached peak value around +20 mV in external solution containing 20 mM Ca2+ at a holding potential of -70 mV. The peak amplitude of ICa increased in a hyperbolic manner with increasing extracellular Ca2+ concentration. The ion selectively was Ba2+ much greater than Ca2+ greater than or equal to Sr2+. It was concluded that the Ca2+ channel in OHCs of guinea pig is of the L-type.
Nakagawa
T
T
Department of Neurophysiology, Tohoku University School of Medicine, Sendai, Japan.
Kakehata
S
S
Akaike
N
N
Komune
S
S
Takasaka
T
T
Uemura
T
T
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D015220:Calcium Channels / Q000502:physiology*; D005260:Female; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D013324:Strontium / Q000494:pharmacology
D015220:Calcium Channels; D001464:Barium; D002118:Calcium; D013324:Strontium