Differential inhibition by Bacillus thuringiensis delta endotoxin of leucine and aspartic acid uptake into BBMV from midgut of Manduca sexta.
Pre-incubation of brush border membrane vesicles (BBMV) isolated from the midgut of Manduca sexta with activated Bacillus thuringiensis delta endotoxin for a short period resulted in differential inhibition of K(+)-dependent transport of leucine relative to the effect on K(+)-dependent transport of aspartic acid. The difference in I1/2 (5 fold greater for aspartic acid than for leucine) is interpreted as the result of enhanced binding of the B. thuringiensis delta endotoxin to the leucine transport system.
Reuveni
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Department of Entomology, Purdue University, West Lafayette, IN 47907-1158.
Dunn
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PE
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001692:Biological Transport / Q000187:drug effects; D004731:Endotoxins / Q000494:pharmacology*; D006460:Hemolysin Proteins; D007408:Intestinal Absorption / Q000187:drug effects; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism*; D009036:Moths / Q000378:metabolism*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D001224:Aspartic Acid; D007930:Leucine