c-erb-A mRNA content and triiodothyronine nuclear receptor binding capacity in rat liver according to vitamin A status.
Vitamin A is required for normal growth and development and synergistically acts with thyroid hormones in these processes. Effects of retinol dietary intake (0, 5, 50 U retinol/g food) on the triiodothyronine (T3) nuclear receptor were examined in rat liver. Properties of this receptor have been investigated by an in vitro binding method. The amount of c-erb-A mRNA has been deduced by reverse transcription and amplification methods (PCR). Results show that both retinol deficiency and excess leads to a reduced amount of c-erb-A mRNA and to a decreased T3 binding capacity, suggesting that retinol, or retinoic acid which is its natural metabolite, contributes to the regulation of the T3 nuclear receptor properties.
Pailler-Rodde
I
I
Laboratoire de Nutrition, ISTAB, Université de Bordeaux I, Talence, France.
Garcin
H
H
Higueret
P
P
Begueret
J
J
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011988:Receptors, Thyroid Hormone / Q000378:metabolism*; D014158:Transcription, Genetic; D014801:Vitamin A / Q000378:metabolism*
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D014801:Vitamin A