Capillary electrophoresis of peptides. Analysis of adrenocorticotropic hormone-related fragments.
Capillary electrophoresis can be used successfully to analyse small peptides to give additional information to that obtained using high-performance liquid chromatography (HPLC). The separation of a modified adrenocorticotropic hormone (4-9) fragment (Org 2766) and several of its fragments was investigated using capillary zone electrophoresis. Prediction of migration in aqueous systems using pKa-related data and the migration behaviour using sodium dodecyl sulphate in the buffer are discussed, as is the choice of buffer systems. The electrophoretic patterns are compared with the HPLC separation.
van de Goor
T A
TA
Laboratory of Instrumental Analysis, Eindhoven University of Technology, The Netherlands.
Janssen
P S
PS
van Nispen
J W
JW
van Zeeland
M J
MJ
Everaerts
F M
FM
D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification*
D010446:Peptide Fragments; C013765:Org 2766; D000324:Adrenocorticotropic Hormone