Purification and characterization of peroxisomal apo and holo alanine:glyoxylate aminotransferase from bird liver.
Alanine:glyoxylate aminotransferase has been reported to be present as the apo enzyme in the peroxisomes and as the holo enzyme in the mitochondria in chick (white leghorn) embryonic liver. However, surprisingly, birds were found to be classified into two groups on the basis of intraperoxisomal forms of liver alanine:glyoxylate aminotransferase. In the peroxisomes, the enzyme was present as the holo form in group 1 (pigeon, sparrow, Java sparrow, Australian budgerigar, canary, goose, and duck), and as the apo form in group 2 (white leghorn, bantam, pheasant, and Japanese mannikin). In the mitochondria, the enzyme was present as the holo form in both groups. The peroxisomal holo enzyme was purified from pigeon liver, and the peroxisomal apo enzyme from chicken (white leghorn) liver. The pigeon holo enzyme was composed of two identical subunits with a molecular weight of about 45,000, whereas the chicken apo enzyme was a single peptide with the same molecular weight as the subunit of the pigeon enzyme. The peroxisomal holo enzyme of pigeon liver was not immunologically cross-reactive with the peroxisomal apo enzyme of chicken liver, the mitochondrial holo enzymes from pigeon and chicken liver, and mammalian alanine:glyoxylate aminotransferases 1 and 2. The mitochondrial holo enzymes from both pigeon and chicken liver had molecular weights of about 200,000 with four identical subunits and were cross-reactive with mammalian alanine:glyoxylate aminotransferase 2 but not with mammalian alanine:glyoxylate aminotransferase 1.
Sakuraba
H
H
Department of Biochemistry, Kyushu Dental College, Kitakyushu, Japan.
Fujiwara
S
S
Noguchi
T
T
D000410:Alanine Transaminase / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D001051:Apoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D001717:Birds / Q000378:metabolism*; D015153:Blotting, Western; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002645:Chickens / Q000378:metabolism; D002845:Chromatography; D002850:Chromatography, Gel; D010856:Columbidae / Q000378:metabolism; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D006882:Hydroxyapatites; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008830:Microbodies / Q000201:enzymology*; D013045:Species Specificity; D013347:Subcellular Fractions / Q000201:enzymology; D000637:Transaminases
D001051:Apoenzymes; D006882:Hydroxyapatites; D017886:Durapatite; D000637:Transaminases; D000410:Alanine Transaminase; C019635:Alanine-glyoxylate transaminase