Quinolone resistance-determining region in the DNA gyrase gyrB gene of Escherichia coli.
Thirteen spontaneous quinolone-resistant gyrB mutants of Escherichia coli KL16, including two that were examined previously, were divided into two types according to their quinolone resistance patterns. Type 1 mutants were resistant to all the quinolones tested, while type 2 mutants were resistant to acidic quinolones and were hypersusceptible to amphoteric quinolones. Nucleotide sequence analysis disclosed that all nine type 1 mutants had a point mutation from aspartic acid to asparagine at amino acid 426 and that all four type 2 mutants had a point mutation from lysine to glutamic acid at amino acid 447.
Yoshida
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H
Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.
Bogaki
M
M
Nakamura
M
M
Yamanaka
L M
LM
Nakamura
S
S
D000595:Amino Acid Sequence; D001665:Binding Sites; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004250:DNA Topoisomerases, Type II / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D015363:Quinolones / Q000494:pharmacology*
D004269:DNA, Bacterial; D015363:Quinolones; D004250:DNA Topoisomerases, Type II