title: Salicylate as an in vivo free radical trap: studies on ischemic insult to the rat intestine.
abstract: Ischemia of rat intestine was induced in vivo by occlusion of the superior mesenteric artery (SMA) for 15 min. Sodium salicylate, 100 mg/kg, given IP, 30 min prior to the ischemic event served as a specific trap for hydroxyl radicals. Portions of the bowel were sequentially isolated and removed--2 min prior to ischemia, 2 min prior to declamping of the SMA, and 10 min following reperfusion. The bowel segments were homogenized in 3% TCA. The homogenate was centrifuged and filtrated through a 0.22 mu filter. The hydroxylation products of salicylate, dihydroxybenzoic acid (DHBA) derivatives, were isolated, identified, and quantified by HPLC coupled with electrochemical detection (ECD). The level of 2,5-DHBA (M +/- SE, ng/g tissue) in the preischemic bowel (N = 21) was 241.8 +/- 10.0. In the ischemic specimen the level of 2,5-DHBA increased significantly to 313.3 +/- 15.5 (p = 0.0129), and remained unchanged in the reperfusion period (322.8 +/- 15.5). The histological examination correlated well with these levels: mild villi damage in the ischemic period with no further exacerbation during the reperfusion period. This study in an in vivo animal model of intestinal ischemia-reperfusion provides direct evidence for the involvement of free radicals during the ischemic insult.
authors.affiliation: Department of Pediatric Surgery, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Udassin
authors.forename: I
authors.initials: I
authors.lastname: Ariel
authors.forename: Y
authors.initials: Y
authors.lastname: Haskel
authors.forename: N
authors.initials: N
authors.lastname: Kitrossky
authors.forename: M
authors.initials: M
authors.lastname: Chevion
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005609:Free Radicals; D005841:Gentisates; D006878:Hydroxides / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000378:metabolism; D017665:Hydroxyl Radical; D007422:Intestines / Q000098:blood supply*; D008297:Male; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D012459:Salicylates / Q000378:metabolism*; D020156:Salicylic Acid
chemical_list: D005609:Free Radicals; D005841:Gentisates; D006878:Hydroxides; D062385:Hydroxybenzoates; D012459:Salicylates; D017665:Hydroxyl Radical; C009135:2,3-dihydroxybenzoic acid; D020156:Salicylic Acid; C010925:2,5-dihydroxybenzoic acid