Detection of HPV-16 DNA in cervical carcinoma by paraffin section in situ hybridization.
Thirty-eight cervical carcinomas and one cervical condyloma were examined for the presence of human papillomavirus type 16 (HPV-16) DNA using 3H-dcTP-labelled HPV-16 DNA probe in paraffin section in situ hybridization and dot blot hybridization (Tm-17 degrees C). The results showed that HPV-16 DNA positive rate in our series was about 74.4% (29/39) as determined by paraffin section in situ hybridization and about 71.8% (28/29) by dot blot hybridization. There was no significant difference between the two methods. We thus confirmed that paraffin section in situ hybridization is an informative, reliable, and sensitive method for the diagnosis of cervical HPV infection.
Zhang
W
W
Department of Cancer Virus, Chinese Academy of Medical Sciences, Beijing.
Shang
M
M
Jin
S Q
SQ
Liang
X
X
Liang
K
K
Sun
Y Z
YZ
Wu
A R
AR
Sun
J H
JH
Nan
G S
GS
Wang
X X
XX
D003218:Condylomata Acuminata / Q000382:microbiology; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D014412:Tumor Virus Infections; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D015342:DNA Probes; D004279:DNA, Viral