Antiviral activity of carrageenan on hepatitis A virus replication in cell culture.
Sulphated polysaccharides such as iota-, lambda- and kappa-carrageenans showed a potent inhibitory effect on the replication of hepatitis A virus (HAV) in the human hepatoma cell line PLC/PRF/5. No cytotoxic effects were detected with concentrations of carrageenans up to 200 micrograms/ml. The selectivity indices of these substances, calculated as the ratio of the dose that reduced the number of viable cells to 50% (CD50) to the effective dose that inhibited 50% of viral antigen expression (ED50), were greater than 400 with iota-carrageenan, greater than 222 with lambda-carrageenan and greater than 10 with kappa-carrageenan. The selectivity index of ribavirin (reference substance) was only 5. The 3 types of carrageenans resulted in concentration-dependent reduction of HAV-antigen expression and HAV infectivity. lota-and lambda-carrageenan emerged, from the present study, as promising candidates for chemotherapy of acute hepatitis A.
Girond
S
S
Université J. Fourier, UFR Pharmacie, Service de Bactériologie-Virologie-Immunologie, La Tronche, France.
Crance
J M
JM
Van Cuyck-Gandre
H
H
Renaudet
J
J
Deloince
R
R
D002351:Carrageenan / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000382:microbiology; D003627:Data Interpretation, Statistical; D004305:Dose-Response Relationship, Drug; D015967:Gene Expression Regulation, Viral; D006506:Hepatitis A / Q000188:drug therapy; D006507:Hepatovirus / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D066298:In Vitro Techniques; D012254:Ribavirin / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D012254:Ribavirin; D002351:Carrageenan