[The application of immunofluorescence and neutralization McAb tests to typing diagnosis of 286 polio strains].
The serum neutralization test is a classical method used for typing poliovirus. Since 1987, some type-specific non-neutralization McAb's have been obtained in our laboratory, and were used in indirect immunofluorescence straining tests to detect serotypes I, II and III. A total of 286 poliovirus strains were typed by the indirect immunofluorescence test (IF) and neutralization test (NT). The results demonstrated that both tests were in agreement, suggesting that McAb IF could replace NT as an alternative typing diagnostic method: moreover, IF was easier to perform than NT. It is economical and rapid (the results can be read within 1-2 days as compared to 7 days for NT). The reagent is very stable and can be stored for two years at 4 degrees C or for two months at 37 degrees C.
Tang
E
E
Institute of Medical Biology, Kunming.
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D005455:Fluorescent Antibody Technique; D009500:Neutralization Tests; D017955:Poliovirus / Q000145:classification*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral