Neutralizing antibodies induced by recombinant vaccinia virus expressing varicella-zoster virus gpIV.
Monoclonal antibodies generated against varicella-zoster virus (VZV) glycoprotein I (gpI) also recognize VZV gpIV (A. Vafai, Z. Wroblewska, R. Mahalingam, G. Cabirac, M. Wellish, M. Cisco, and D. Gilden, J. Virol. 62:2544-2551, 1988). To determine whether the virus-neutralizing activity of these antibodies belongs to gpI, gpIV, or both, the open reading frame encoding gpIV was inserted into the vaccinia virus genome. Immunoprecipitation of recombinant vaccinia virus-infected cells with anti-gpIV monoclonal antibody yielded synthesis and processing of gpIV similar to those expressed in VZV-infected cells. Antibodies raised against VVgpIV in a rabbit recognized both native gpI and gpIV and neutralized VZV infectivity. In addition, antibodies raised against recombinant vaccinia virus carrying VZV gpI neutralized VZV infection. These results indicate a structural relationship between VZV gpI and gpIV and show that gpI and gpIV each induce virus-neutralizing antibody.
Vafai
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Department of Biomedical Sciences, University of Illinois College of Medicine, Rockford 61107.
Yang
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005814:Genes, Viral; D005821:Genetic Techniques; D014645:Herpesvirus 3, Human / Q000235:genetics*; D009500:Neutralization Tests; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D014759:Viral Envelope Proteins; C070916:glycoprotein IV, varicella-zoster virus