Immunochemical demonstration of alpha 3 isozyme of Na,K-ATPase in human brain.
The catalytic alpha (alpha) subunit of the human Na,K-ATPase is encoded by multiple genes, as revealed from molecular cloning studies, yet the presence of the alpha 3 isoform has still not been resolved. We investigated the human alpha 3 isoform by Western blotting employing polyclonal anti-rat brain alpha 3 fusion protein, and purified human and rat axolemma Na,K-ATPase preparations. The antibody to rat brain alpha 3 fusion protein cross-reacted with both human and rat antigens, and the stained band superimposed on the alpha 2 band. This result suggests that alpha 3 isoform also exists in human brain.
Peng
J H
JH
University of Missouri-Kansas City School of Medicine, Truman Medical Center 64108.
Parker
J C
JC
Tsai
F Y
FY
D000818:Animals; D000918:Antibody Specificity; D001369:Axons / Q000201:enzymology; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007527:Isoenzymes; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification
D007527:Isoenzymes; D009243:NAD; D000254:Sodium-Potassium-Exchanging ATPase