Photoreactivation of ultraviolet radiation-induced release of arachidonic acid from marsupial cells.
Exposure of an established marsupial cell line, PtK2 (Potorous tridactylus), to ultraviolet radiation (UVR) from an FS-40 sunlamp (280-400 nm) resulted in a fluence-dependent release of radiolabeled arachidonic acid (AA) from cell membranes. Post-UVR, but not pre-UVR, exposure to photoreactivating light reversed UVR-induced pyrimidine dimers in DNA and suppressed the UVR-induced release of AA. These data indicate that DNA damage contributes to the release of AA from membrane phospholipids.
Kaleta
E W
EW
Center for Photomedicine, Lovelace Medical Foundation, Albuquerque, NM 87108.
Applegate
L A
LA
Ley
R D
RD
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002460:Cell Line; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000502:physiology*; D004307:Dose-Response Relationship, Radiation; D004720:Endonucleases / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000528:radiation effects; D007614:Macropodidae; D008563:Membrane Lipids / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011740:Pyrimidine Dimers / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
D008563:Membrane Lipids; D010743:Phospholipids; D011740:Pyrimidine Dimers; D016718:Arachidonic Acid; D004720:Endonucleases