Sequence analysis of the splice junction in the transcript of herpes simplex virus type 1 gene UL15.
Gene UL15 of herpes simplex virus type 1 has been proposed to consist of two coding exons separated by an intron of 3587 base pairs. We have generated a DNA fragment copied from the transcript across the proposed splice junction by successive use of reverse transcription and the polymerase chain reaction, and have sequenced this fragment to determine the precise structure of the splice junction.
Dolan
A
A
Institute of Virology, University of Glasgow, U.K.
Arbuckle
M
M
McGeoch
D J
DJ
D001483:Base Sequence; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D005091:Exons; D005814:Genes, Viral; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D004279:DNA, Viral