title: Heterogeneity of immunoreactive dynorphin B-like material in human, rat, rabbit and guinea-pig heart.
abstract: Immunoreactive dynorphin B-like material (ir-dyn B) was detected in acetic acid extracts of human atrial specimens and of rat, rabbit and guinea-pig atria and ventricles by a validated radioimmunoassay. Levels were high in rabbit atrium (66.76 +/- 7.04 pmol/g) but lower and superimposable in human and rat atria (28.18 +/- 3.20 and 30.22 +/- 2.45 pmol/g, respectively). Gel permeation chromatography revealed ir-dyn B eluting close to column exclusion and in forms with an apparently higher molecular weight than authentic dyn B in human and rat samples. In contrast, almost all the immunoreactivity from rabbit and guinea-pig acetic extracts eluted as a single peak in the region of standard dyn B. Reverse-phase high performance liquid chromatography of the pooled gel chromatography fractions of this peak showed up a molecular form with the same retention time as authentic dyn B and a second minor peak of unknown immunoreactive material eluting three fractions earlier. Digestion with carboxypeptidase B excluded the hypothesis that this latter could be dyn B-Arg14. Therefore, it might be a metabolite of endogenous dyn B recognized by the antibody used in this study.
authors.affiliation: Institute of Pharmacology, University of Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Spampinato
authors.forename: M
authors.initials: M
authors.lastname: Canossa
authors.forename: C
authors.initials: C
authors.lastname: Ventura
authors.forename: T
authors.initials: T
authors.lastname: Bachetti
authors.forename: R
authors.initials: R
authors.lastname: Venturini
authors.forename: L
authors.initials: L
authors.lastname: Bastagli
authors.forename: P
authors.initials: P
authors.lastname: Bernardi
authors.forename: S
authors.initials: S
authors.lastname: Ferri
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins / Q000032:analysis*; D006168:Guinea Pigs; D006325:Heart Atria / Q000737:chemistry; D006352:Heart Ventricles / Q000737:chemistry; D006801:Humans; D007031:Hypothalamus / Q000737:chemistry; D008297:Male; D009206:Myocardium / Q000737:chemistry*; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
chemical_list: D004399:Dynorphins