The effects of insulin and the pituitary peptide beta-cell tropin on the incorporation of D-3-3H-glucose into lipid in brown adipocytes from lactating and non-lactating rats.
Lactating and non-lactating rat brown adipocytes were used to study the dose-dependent stimulation of lipogenesis by Beta-cell tropin (BCT) and insulin. In non-lactating animals BCT increased lipogenesis approximately 2-fold compared to a 3-fold stimulation with insulin; however BCT was effective at a substantially lower molar concentration than insulin. In lactating animals resistance was observed to both BCT and insulin action.
Davenport
M
M
Clore Laboratory for the Biological Sciences, University of Buckingham, Bucks, U.K.
Morton
J L
JL
Beloff-Chain
A
A
Dunmore
S J
SJ
Cawthorne
M A
MA
D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D007774:Lactation / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014316:Tritium
D007328:Insulin; D008055:Lipids; D010446:Peptide Fragments; D014316:Tritium; C026834:beta-cell tropin; D000324:Adrenocorticotropic Hormone; D005947:Glucose