title: DBcAMP effect on the expression of GFAP and of its encoding mRNA in astroglial primary cultures.
abstract: Short term and chronic dBcAMP effects on the expression of glial fibrillary acidic protein (GFAP) in astroglial primary cultures are investigated. Short (48 h) and long (more than 7 days) treatments with the cAMP derivative induce both cell shape changes and an increase in GFAP immunolabelling. Such effects are only associated with an increase in GFAP and in GFAP-mRNA levels in the long term treatment. These results suggest that the short term effect of dBcAMP induces post-translational modifications of the protein whereas the long term effect is associated with an increase in GFAP mRNA transcription and/or stability.
authors.affiliation: INSERM U 282, Hôpital Henri Mondor, Creteil, France.
authors.forename: G
authors.initials: G
authors.lastname: Le Prince
authors.forename: C
authors.initials: C
authors.lastname: Fages
authors.forename: B
authors.initials: B
authors.lastname: Rolland
authors.forename: J
authors.initials: J
authors.lastname: Nunez
authors.forename: M
authors.initials: M
authors.lastname: Tardy
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D003994:Bucladesine / Q000494:pharmacology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis*; D051379:Mice; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D003994:Bucladesine