title: [Isolation of rat hepatocytes by a nonenzymatic method: detoxifying and respiratory activity].
abstract: A procedure for isolation of rat hepatocytes involving perfusion by EDTA, mechanical disaggregation of the liver and separation of intact cells from damaged ones by low-velocity centrifugation in isotonic sucrose media is described. A layer of a darker colour formed after separation contains hepatocytes with native plasma membrane characterized by respiratory activity and rate of biotransformation being close to the values obtained with cells isolated with the application of collagenase.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Petrenko
authors.forename: A N
authors.initials: AN
authors.lastname: Sukach
authors.forename: A D
authors.initials: AD
authors.lastname: Rosliakov
mesh_terms: D000818:Animals; D001711:Biotransformation; D002469:Cell Separation; D004492:Edetic Acid; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D003012:Microbial Collagenase / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004492:Edetic Acid; D003012:Microbial Collagenase; D010100:Oxygen
vernacular_title: Vydelenie gepatotsitov krys nefermentativnym metodom: detoksikatsionnaia i dykhatel'naia aktivnosti.