Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin.
Porcine secretin, [Tyr10] secretin, and [Tyr13] secretin were synthesized by solid phase methodology and purified by stepwise gradient elution from a short reversed-phase column with ethanol and acetic acid as organic modifiers. [Tyr10] secretin and [Tyr13] secretin were iodinated by the chloramine-T method and nonmono-, and di-iodinated products separated and isolated by reversed-phase HPLC. Batch incubation analysis is isolated mouse pancreatic islets revealed that secretin and the [Tyr10] analogue were indistinguishable in their effect on the glucose-induced insulin release and cAMP accumulation. [Tyr13] secretin in contrast was significantly less potent in its effect on the glucose-induced insulin release.
Kofod
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Hagedorn Research Laboratory, Gentofte, Denmark.
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000032:analysis; D007328:Insulin / Q000032:analysis; D051379:Mice; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D012633:Secretin / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology; D013552:Swine
D007328:Insulin; D012633:Secretin; C070149:secretin, -Tyr(10)-; D000242:Cyclic AMP