NGF induces activation of phospholipase C (membrane bound) in PC12 cells.
The aim of this work is to examine if the membrane-bound Phospholipase C system is correlated with the differentiative action of Nerve Growth Factor in PC12 cells. We found that the activity of membrane-bound Phospholipase C system increased with the presence of Nerve Growth Factor at two different phases. The early phase occurs during the first minutes after the formation of Nerve Growth Factor-receptor complex and it is completed within one hour. The later phase starts two hours after Nerve Growth Factor introduction and lasts for at least a total of 48 hours. The inositol-triphosphate levels measured in intact cells by radioimmunoassay show the same pattern of activation. We think that this dual response to Nerve Growth Factor could be due to either a double separated activation of the same enzyme or the presence of two different forms of membrane-bound Phospholipase C.
Belia
S
S
Istituto di Biologia Cellulare, Perugia, Italy.
Fulle
S
S
Antonioli
S
S
Salvatore
A M
AM
Fano
G
G
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D007295:Inositol Phosphates / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells; D010716:Phosphatidylinositols / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D010738:Type C Phospholipases / Q000378:metabolism*
D007295:Inositol Phosphates; D009414:Nerve Growth Factors; D010716:Phosphatidylinositols; D010738:Type C Phospholipases