title: Effect of sodium chloride on transport of bacteria in a saturated aquifer material.
abstract: Determinations were made of the influence of NaCl concentration, cell density, and flow velocity on the transport of Pseudomonas sp. strain KL2 through columns of aquifer sand under saturated conditions. A pulse-type boundary condition was used. The experiments were conducted by using 0.3-m-long Plexiglas columns with an internal diameter of 0.05 m. When a 1-h pulse of a 0.01 M NaCl solution containing 10(8) cells per ml was added at a flow rate of 10(-4) m s-1, the bacterial density in the effluent never exceeded 2.2% of the density of cells added, and only 1.5% of the bacteria passed through the aquifer material. In contrast, when the bacteria were applied in distilled water, the relative cell density in the effluent approached 100%, and 60% of the bacteria were transported through the aquifer solids. Under these conditions, the breakthrough of Pseudomonas sp. strain KL2 was slower than chloride. When the flow rate was 2.0 x 10(-4) m s-1, the cell density in the effluent reached 7.3% of that added in 0.01 M NaCl solution, but only 3.9% of the bacteria were transported through the aquifer particles. On the other hand, the density in the effluent approached 100% of that added in deionized water, and 77% of the added bacteria were recovered. When the density of added cells was 10(9) cells per ml at a flow rate of 10(-4) m s-1, the densities in the effluent reached 70 and 100% of those added in salt solution and deionized water, respectively, and 44 and 57% of the bacteria were transported through the aquifer solids.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Soil, Crop, and Atmospheric Sciences, Cornell University, Ithaca, New York 14853.
authors.forename: J
authors.initials: J
authors.lastname: Gannon
authors.forename: Y H
authors.initials: YH
authors.lastname: Tan
authors.forename: P
authors.initials: P
authors.lastname: Baveye
authors.forename: M
authors.initials: M
authors.lastname: Alexander
mesh_terms: D002465:Cell Movement / Q000187:drug effects; D003470:Culture Media; D011549:Pseudomonas / Q000187:drug effects / Q000502:physiology*; D012822:Silicon Dioxide; D012965:Sodium Chloride / Q000494:pharmacology*; D012988:Soil Microbiology
chemical_list: D003470:Culture Media; D012965:Sodium Chloride; D012822:Silicon Dioxide