Ion-exchange high-performance liquid chromatography of nucleotides and polypeptides on new types of ion-exchange sorbents, based on polystyrene-coated silicas.
A novel type of ion exchanger was prepared by multipoint covalent binding of polystyrene chains onto the surface of porous silica followed by polymer-analogous modification of the bonded layer. Both anion and cation exchangers were synthesized and examined in the separation of nucleotides and proteins. Rapid and efficient separation of basic polypeptides on strong anion exchangers and that of acidic polypeptides on strong cation exchangers could be achieved. For the separation of complete mixtures of polypeptides the application of zwitter-ionic ion exchangers can be recommended.
Kurganov
A A
AA
Nesmeyanov Institute of Organo-Element Compounds, Academy of Sciences of USSR, Moscow.
Davankov
V A
VA
Unger
K K
KK
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D006801:Humans; D007475:Ion Exchange Resins / Q000737:chemistry*; D009711:Nucleotides / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*; D011137:Polystyrenes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
D007475:Ion Exchange Resins; D009711:Nucleotides; D010455:Peptides; D011137:Polystyrenes; D012822:Silicon Dioxide