Microtubule destabilization by cdc2/H1 histone kinase: phosphorylation of a "pro-rich region" in the microtubule-binding domain of MAP-4.
Microtubule-associated protein-4 (MAP-4), a major MAP in proliferating cells, consists of a microtubule-binding domain and a projection domain protruding from the microtubule wall. The former contains a Pro-rich region and an assembly-promoting (AP) sequence region which is common to the neuron-specific MAPs, MAP-2 and tau1. In this paper, we describe the phosphorylation of the Pro-rich region of MAP-4 and the suppression of its assembly-promoting activity by cdc2/H1 histone kinase. This inactivation of MAP-4 may cause disassembly of the interphase microtubular network at the end of the G2 phase of the cell cycle.
Aizawa
H
H
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Kamijo
M
M
Ohba
Y
Y
Mori
A
A
Okuhara
K
K
Kawasaki
H
H
Murofushi
H
H
Suzuki
K
K
Yasuda
H
H
D000595:Amino Acid Sequence; D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002460:Cell Line; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D010455:Peptides / Q000138:chemical synthesis; D010766:Phosphorylation; D006656:Protamine Kinase / Q000378:metabolism*; D014981:Xenopus
D010455:Peptides; D006656:Protamine Kinase; D016203:CDC2 Protein Kinase