The elimination of mouse hepatitis virus by temporary transplantation of human tumors from infected athymic nude mice into athymic nude rats (rnuN/rnuN).
A nude mouse colony held in an isolation unit was found to harbor MHV despite the fact that all hygienic precautions were taken. The virus spread rapidly causing a high mortality rate predominantly in experimental animals. Moreover, we observed a high percentage of tumor regression in our tumor transplanted mice. Attempts to eliminate the MHV by repeated tumor transplantation into virus-free nude mice were unsuccessful. Since MHV has a limited host range, we transplanted, in parallel, four different lines of embryonic renal tumors (three triphasic nephroblastomas and one malignant rhabdoid tumor of the kidney) from athymic mice into athymic rats and fragments of the same tumors into "fresh" nude mice. All manipulations were performed in isolators. Detection of MHV was done twice by serological examination of six-week-old sentinels. The results showed transmission of MHV infection in the control mice under gnotobiotic conditions as previously found in the normal animal room. On the other hand, there was no evidence of infection, neither in the transplanted nude rats nor after retransplantation of tumors into nude mice. We hypothesize that the virus is harbored in the stromal cells of the murine host but not of the rat host nor in the human tumor cells. Histological comparison showed no alteration of specific tumor morphology in the different hosts.
Rülicke
T
T
Biological Central Laboratory, University Hospital of Zürich, Switzerland.
Hassam
S
S
Autenried
P
P
Briner
J
J
D000818:Animals; D005260:Female; D006524:Hepatitis, Viral, Animal / Q000628:therapy*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D006517:Murine hepatitis virus / Q000472:pathogenicity*; D009368:Neoplasm Transplantation / Q000276:immunology*; D051381:Rats; D011923:Rats, Nude; D013047:Specific Pathogen-Free Organisms