title: Heterogeneity of three electrophoretically distinct Go alpha-subunits in mammalian brain.
abstract: So far three splice variants of the alpha o-gene coding for two alpha o proteins have been identified by molecular cloning, and the corresponding proteins purified. In the present study subtype-specific peptide antibodies revealed the existence of an electrophoretically distinct third form of alpha o in mammalian brain membranes which migrates more slowly on SDS-PAGE and shows a more acidic pI value than the other alpha o-subunits. Each of the three alpha o-subunits is detected as two isoforms when enriched from brain membranes. Rodent alpha o-subunits differ from non-rodent species in their electrophoretic mobilities. The results suggest that (i) there may exist a novel alpha o-subunit which reacts with an alpha o1-subunit-specific antibody, (ii) each alpha o-subunit may exist in more than one co- or posttranslationally modified isoform in brain membranes, and (iii) differences between alpha o-subunits from different species exist which are detectable by gel electrophoretic methods.
authors.affiliation: Institut für Pharmakologie, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Spicher
authors.forename: B
authors.initials: B
authors.lastname: Nuernberg
authors.forename: B
authors.initials: B
authors.lastname: Jäger
authors.forename: W
authors.initials: W
authors.lastname: Rosenthal
authors.forename: G
authors.initials: G
authors.lastname: Schultz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001923:Brain Chemistry; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D015151:Immunoblotting; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D013552:Swine
chemical_list: D019204:GTP-Binding Proteins