Expression cloning of an adenylate cyclase-coupled calcitonin receptor.
A calcitonin receptor complementary DNA (cDNA) was cloned by expression of a cDNA library from a porcine kidney epithelial cell line in COS cells. The 482-amino acid receptor has high affinity for salmon calcitonin (dissociation constant Kd approximately 6 nM) and is functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP). The receptor shows no sequence similarity to other reported G protein-coupled receptors but is homologous to the parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) receptor, indicating that the receptors for these hormones, which regulate calcium homeostasis, represent a new family of G protein-coupled receptors.
Lin
H Y
HY
Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Harris
T L
TL
Flannery
M S
MS
Aruffo
A
A
Kaji
E H
EH
Gorn
A
A
Kolakowski
L F
LF
Lodish
H F
HF
Goldring
S R
SR
D000262:Adenylyl Cyclases / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002116:Calcitonin / Q000378:metabolism*; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000502:physiology; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D007668:Kidney / Q000502:physiology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface / Q000235:genetics*; D013552:Swine
D012333:RNA, Messenger; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface; D002116:Calcitonin; D004247:DNA; D000242:Cyclic AMP; D000262:Adenylyl Cyclases