The recombinant human interferon-gamma receptor is fully functional in a human x murine hybrid containing human chromosome 21.
It has previously been shown that stimulation of class I MHC antigen expression by human interferon gamma (Hu-IFN gamma) depends on the presence of both human chromosomes 6 and 21. Even although the presence of human chromosome 6 results in normal expression of the IFN gamma receptor on the cell surface, chromosome 21 is required for this biological response. Here, we transfected the cDNA encoding the Hu-IFN gamma receptor into a mouse cell line, L(tk-), and into a mouse x human hybrid, GM08, containing the human chromosome 21 as the only human genetic material. Two clones, L-gamma R.8 and GM08-gamma R.9, were tested for different biological responses to Hu-IFN gamma. Only the hybrid containing the human chromosome 21 (GM08-gamma R.9) exhibited a biological response when assayed for the stimulation of murine class I MHC antigen expression by Hu-IFN gamma. Moreover, transcriptional induction of 2'-5'-oligoadenylate synthetase and 1-8 genes, as well as antiviral protection, were observed only in the GM08-gamma R.9 transfectants. These results show that human chromosome 21 encodes a factor which mediates different biological activities of Hu-IFN gamma.
Bono
M R
MR
Institut National de la Santé et de la Recherche Médicale U.276, Paris, France.
Alcaïde-Loridan
C
C
Letouzé
B
B
Jouin
H
H
Gobin
S J
SJ
Fellous
M
M
D015152:Blotting, Northern; D002891:Chromosomes, Human, Pair 21; D005786:Gene Expression Regulation; D005805:Genes, MHC Class I / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D007371:Interferon-gamma / Q000494:pharmacology*; D011971:Receptors, Immunologic / Q000235:genetics / Q000502:physiology*; D017471:Receptors, Interferon; D011994:Recombinant Proteins / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D014162:Transfection / Q000235:genetics; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology
D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D007371:Interferon-gamma