title: Differential distribution of a glucuronyltransferase, involved in glucuronoxylan synthesis, within the Golgi apparatus of pea (Pisum sativum var. Alaska).
abstract: The subcellular location of a glucuronyltransferase (GT) involved in glucuronoxylan synthesis in pea (Pisum sativum) has been investigated. Most of the GT activity was found in the Golgi fraction, but activity was also detected in the plasma-membrane fraction. Separation of Golgi membranes on a shallow continuous sucrose density gradient resulted in three distinct subfractions, with GT activity being confined to Golgi membranes of a density similar to that of smooth endoplasmic reticulum. The differential distribution of GT within the Golgi stack indicates that glucuronoxylan synthesis occurs in specific cisternae and that there is functional compartmentalization of the Golgi with respect to hemicellulose biosynthesis.
authors.affiliation: Department of Botany, University of Glasgow, Scotland, U.K.
authors.forename: M C
authors.initials: MC
authors.lastname: Hobbs
authors.forename: M H
authors.initials: MH
authors.lastname: Delarge
authors.forename: E A
authors.initials: EA
authors.lastname: Baydoun
authors.forename: C T
authors.initials: CT
authors.lastname: Brett
mesh_terms: D017766:Acid Anhydride Hydrolases; D002451:Cell Compartmentation; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D007887:Fabaceae / Q000201:enzymology*; D005964:Glucosyltransferases / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006056:Golgi Apparatus / Q000201:enzymology* / Q000648:ultrastructure; D009245:NADH Dehydrogenase / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010946:Plants, Medicinal; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014990:Xylans / Q000096:biosynthesis*
chemical_list: D014990:Xylans; C038910:glucuronoxylan; D013385:Succinate Dehydrogenase; D009245:NADH Dehydrogenase; D005964:Glucosyltransferases; C040232:glucan synthase; D014453:Glucuronosyltransferase; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; C021446:inosine diphosphatase