[Typing of papillomaviruses in cervical dysplasias. Its value in treatment].
The typing fo Human Papillomavirus (HPV) was carried out in cervical lesions in order to decide on the best therapy to carry out in mild and medium dysplasias of the cervix. 131 patients who had an iodo-negative zone or a smear suggesting an HPV infection had microbiopsies carried out under colposcopic control. Every lesion had two biopsies carried out side by side to study the histopathology and the virology. Dysplastic lesions were found in 93 biopsies. A search for the DNA of HPV 6a, 16 and 18 was carried out using a Southern blot technique with 32P following the method of Random multipriming. Hybridization was carried out in strict and non-strict conditions. In 43 cases out of 93 dysplasias a virus was found: 2 HPV 6a, 14 HPV 16, 1 HPV 18 and 26 X HPV's (not typed). The number of HPV's that could be detected increased according to the severity of the dysplasia which was also found in frequency of type 16. Where the biopsies showed no dysplasia type 16 was found in four out of seven viruses. In this study electrophoretic profiles for type 16 were found in four cases suggesting that they were integrated into the chromosome of the cell. The low percentage of HPV found in mild or moderate dysplasias (4 type 16 out of 61 biopsies) shows that at present this test can not be used as an aid in choosing treatment.
Billaudel
S
S
Laboratoire de Virologie, CHRU Nantes, Hôtel-Dieu.
Aillet
G
G
Tardivel
P
P
Besse
B
B
Sagot
P
P
Le Guyader
F
F
Mensier
A
A
Lopes
P
P
D000328:Adult; D001706:Biopsy; D015139:Blotting, Southern / Q000379:methods / Q000592:standards*; D003127:Colposcopy / Q000592:standards; D005069:Evaluation Studies as Topic; D005260:Female; D005602:France / Q000453:epidemiology; D006801:Humans; D015994:Incidence; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification*; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000382:microbiology* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000382:microbiology* / Q000473:pathology
Typage des papillomavirus dans les dysplasies du col. Intérêt pour leur traitement.