The majority of cells infected with the defective murine AIDS virus belong to the B-cell lineage.
Murine AIDS (MAIDS) is caused by a defective retrovirus which encodes a gag fusion protein (Pr60gag). We previously reported that this virus induced an oligoclonal proliferation of infected cells and suggested that this cell expansion was an important event in the pathogenesis of MAIDS. To identify these target cells, we constructed novel defective viruses whose genomes could be detected with specific probes. Helper-free stocks of these viruses induced MAIDS. Using in situ hybridization and immunocytochemistry and Southern analysis, we found that most infected cells belong to the B-cell lineage. Transformation of these B cells appears to be the primary event responsible for the development of immunodeficiency. This animal model may be relevant to our understanding of AIDS, of the immunodeficiencies associated with B-cell lymphoproliferative disorders, and of the role of B-cell proliferation and transformation in the effects of superantigens, since Pr60gag appears to be a superantigen.
Huang
M
M
Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Quebec, Canada.
Simard
C
C
Kay
D G
DG
Jolicoeur
P
P
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D003673:Defective Viruses / Q000235:genetics* / Q000276:immunology; D015321:Gene Rearrangement; D009052:Leukemia Virus, Murine / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity
D015342:DNA Probes; D004279:DNA, Viral; D004247:DNA