[The adaptation of an isolate of the hepatitis A virus to reproduction in cell cultures].
A HAV strain derived from a patient in Moscow multiplied in a continuous cell line PLC/PRF/5 at 32 degrees C (variant MI-1) and at 37 degrees C (variant MI-1.1). These two variants of the strain MI retained the ability for reproduction both at 32 degrees C and 37 degrees C after passages in cell culture. The strain MI also replicates in MK cells. Negative results on HAV cultivation were obtained in HEF-240 and FRhK-4 cell cultures. The MI-1 and MI-1.1 variants are typical of hepatitis A viruses by their physicochemical properties, the results of ELISA, protein electrophoresis and dot hybridization tests.
Farashian
V R
VR
Bychenko
A B
AB
Kuzaeva
I A
IA
Artemov
E K
EK
Kadoshnikov
Iu P
IuP
D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000382:microbiology; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D006506:Hepatitis A / Q000382:microbiology; D006507:Hepatovirus / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D008253:Macaca mulatta; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication / Q000502:physiology*
D004279:DNA, Viral; D014764:Viral Proteins
Adaptatsiia izoliata virusa gepatitia A k reproduktsii v kletochnykh kul'turakh.