Factors affecting the digestion of restriction endonucleases in situ.
The influence of incubation buffers and glycerol on the enzyme activity of naked DNA of lambda phage and mouse, and of mouse chromosomal DNA was investigated. The results obtained varied in part from previously known data, but confirmed the importance of these factors in determining the patterns of in situ restriction enzyme digestion so far attributed exclusively to endonuclease activity.
Ferrucci
L
L
Dipartimento di Biologia, Universita' di Tor Vergata, Roma, Italy.
Rossino
R
R
Mezzanotte
R
R
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002021:Buffers; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005990:Glycerol / Q000378:metabolism*; D051379:Mice
D002021:Buffers; D004279:DNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes; D005990:Glycerol