Alpha-melanocyte-stimulating hormone reduces interleukin-1 beta effects on rat stomach preparations possibly through interference with a type I receptor.
Contractions elicited by CaCl2 on isolated rat stomach strip preparations have been reported to be potentiated by interleukin-1 beta (IL-1 beta). We have investigated whether this effect can be reduced by the putative IL-1 beta antagonist, alpha-melanocyte-stimulating hormone (alpha MSH). Additionally, the effects of alpha MSH on the specific binding of IL-1 beta to B- and T-cells have been investigated to further clarify its inhibitory activities. Both alpha MSH and its carboxyl terminal tripeptide concentration dependently reduced the potentiation of CaCl2-induced contractions caused by IL-1 beta but not those caused by leukotriene D4, the parent molecule being approximately 250 times more active. Additionally, both peptides potently and selectively reduced 125I-IL-1 beta binding to the T-cell sub-clone EL4-6.1 but not to the B-cell sub-clone 1H7. The results indicate that IL-1 beta effects on rat stomach may be mediated through a type-I (80 kDa) IL-1 beta receptor.
Mugridge
K G
KG
Sclavo Research Centre, Siena, Italy.
Perretti
M
M
Ghiara
P
P
Parente
L
L
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002999:Clone Cells / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D009074:Melanocyte-Stimulating Hormones / Q000031:analogs & derivatives / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000187:drug effects* / Q000502:physiology; D017472:Receptors, Interleukin-1; D013189:SRS-A / Q000494:pharmacology; D013270:Stomach / Q000187:drug effects* / Q000502:physiology; D000521:alpha-MSH / Q000494:pharmacology*
D007375:Interleukin-1; D010446:Peptide Fragments; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D013189:SRS-A; D000521:alpha-MSH; C043156:MSH (11-13); D009074:Melanocyte-Stimulating Hormones; D002122:Calcium Chloride