Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose.
Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogenous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.
Bustos
J
J
Laboratorio de Virología, Edificio A Segundo Piso, Facultad de Medicina, Universidad Nacional Autónoma de México, México City.
Zamora
P
P
Mejía
E
E
Varela
Y
Y
Gómez
B
B
D002500:Centrifugation, Isopycnic / Q000379:methods*; D005069:Evaluation Studies as Topic; D011205:Povidone; D012412:Rubella virus / Q000302:isolation & purification*; D012822:Silicon Dioxide; D013395:Sucrose
D013395:Sucrose; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone