title: Neutrophil chemotactic factor produced by Japanese encephalitis virus stimulated macrophages.
abstract: The mechanism of neutrophil leucocytosis in cases of Japanese encephalitis is not known. We here report that during Japanese encephalitis virus (JEV) infection in mice the splenic macrophages secrete a chemotactic factor that attracts the neutrophils. The peak activity of macrophage derived factor (MDF) was observed on day 7 following infection. The MDF acted in a dose-dependent manner. This chemoattractant was purified by low pressure liquid chromatography and gave a single band of 10 kD on silver stained polyacrylamide gel. The MDF was found to be heat resistant and sensitive to prolonged incubation with proteases.
authors.affiliation: Postgraduate Department of Microbiology, K. G. Medical College, Lucknow, India.
authors.forename: N
authors.initials: N
authors.lastname: Khanna
authors.forename: M
authors.initials: M
authors.lastname: Agnihotri
authors.forename: A
authors.initials: A
authors.lastname: Mathur
authors.forename: U C
authors.initials: UC
authors.lastname: Chaturvedi
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology*; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000737:chemistry; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D013154:Spleen / Q000166:cytology
chemical_list: D016209:Interleukin-8