Dominant low responsiveness in the IgG response of mice to the complex protein antigen type 1 fimbriae from Actinomyces viscosus T14V.
Type 1 fimbriae from Actinomyces viscosus T14V, composed of a complex protein of Mr 65,000, mediate the adherence of A. viscosus T14V to the host, whereas type 1 fimbriae-specific antibodies inhibit adherence. Genetic control of the serum IgG response to type 1 fimbriae was evaluated in a series of inbred, hybrid, recombinant inbred, and back-cross mice. Mice were given i.p. injections of 10(8) A. viscosus T14V cells in saline on days 0 and 14, and IgG anti-type 1 fimbriae in sera obtained on day 26 were measured by ELISA. Segregation analysis of the responses of (BALB/cJ x A/J)F1 x A/J backcross mice suggested polygenic control. Linkage analysis in (BALB/cJ x A/J)F1 x A/J backcross and SWXL recombinant inbred mice suggested control by genes linked with H-2 and with Ly-17 and Akp-1. In several F1 hybrid strains derived from H-2-disparate high and low responder parental strains, low responsiveness was dominant. The F1 derived from the H-2-identical high and low responder strains CBA/J and C3H/HeJ was a low responder, suggesting that dominant low responsiveness was mediated by non-H-2-linked genes. A three-gene model is proposed for regulation of the type 1 fimbriae response, including an MHC-linked gene, a gene linked with Ly-17 and Akp-1 on the telomeric portion of chromosome 1, and a background gene whose linkage is unknown.
Haber
J
J
Department of Oral Pathology, School of Dental Medicine, Tufts University, Boston, MA 02111.
Paradis
G
G
Grinnell
C M
CM
D000190:Actinomyces / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000950:Antigens, Ly / Q000235:genetics; D001426:Bacterial Proteins / Q000276:immunology*; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006183:H-2 Antigens / Q000235:genetics; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000950:Antigens, Ly; D001426:Bacterial Proteins; D006183:H-2 Antigens; D007074:Immunoglobulin G