title: The maleless protein associates with the X chromosome to regulate dosage compensation in Drosophila.
abstract: The maleless (mle) gene is one of four known regulatory loci required for increased transcription (dosage compensation) of X-linked genes in D. melanogaster males. A predicted mle protein (MLE) contains seven short segments that define a superfamily of known and putative RNA and DNA helicases. MLE, while present in the nuclei of both male and female cells, differs in its association with polytene X chromosomes in the two sexes. MLE is associated with hundreds of discrete sites along the length of the X chromosome in males and not in females. The predominant localization of MLE to the X chromosome in males makes it a strong candidate to be a direct regulator of dosage compensation.
authors.affiliation: Department of Biological Sciences, Stanford University, California 94305.
authors.forename: M I
authors.initials: MI
authors.lastname: Kuroda
authors.forename: M J
authors.initials: MJ
authors.lastname: Kernan
authors.forename: R
authors.initials: R
authors.lastname: Kreber
authors.forename: B
authors.initials: B
authors.lastname: Ganetzky
authors.forename: B S
authors.initials: BS
authors.lastname: Baker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002451:Cell Compartmentation; D002868:Chromosomal Proteins, Non-Histone; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004265:DNA Helicases / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D004268:DNA-Binding Proteins; D004303:Dosage Compensation, Genetic; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D007301:Insect Hormones / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014158:Transcription, Genetic; D014960:X Chromosome
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D007301:Insect Hormones; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C070837:mle protein, Drosophila; D004265:DNA Helicases