title: Detection of infectious laryngotracheitis virus in chickens using a non-radioactive DNA probe.
abstract: A DNA hybridization assay using a non-radioactive probe has been developed for the detection of infectious laryngotracheitis virus (ILTV) DNA. A 1.4-kilobase pair BamHI fragment of ILTV genomic DNA was cloned and then labeled by one of two methods; nick translation using 32P-dATP or non-radioactive labeling using a commercially available DNA labeling and detection kit. The non-radioactive DNA labeling method proved to be as sensitive as the radioactive method. Using the non-radioactive probe, ILTV DNA was readily detected in tracheal samples from acutely infected chickens and also from convalescent chickens at a time when viral antigen could no longer be detected by the enzyme-linked immunosorbent assay or the virus could no longer be reisolated. This technique provides a safe and effective means of identifying field outbreaks of ILTV and also may detect latent ILTV infections relatively quickly and inexpensively.
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation, Division of Animal Health, Parkville, Victoria, Australia.
authors.forename: L
authors.initials: L
authors.lastname: Keam
authors.forename: J J
authors.initials: JJ
authors.lastname: York
authors.forename: M
authors.initials: M
authors.lastname: Sheppard
authors.forename: K J
authors.initials: KJ
authors.lastname: Fahey
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002645:Chickens; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D001352:Herpesvirus 1, Gallid / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology*; D011237:Predictive Value of Tests; D013047:Specific Pathogen-Free Organisms; D014132:Trachea / Q000382:microbiology
chemical_list: D000956:Antigens, Viral; D015342:DNA Probes; D004279:DNA, Viral