Selective reduction of [125I]apamin binding sites in Alzheimer hippocampus: a quantitative autoradiographic study.
[125I]Apamin binding sites were examined using quantitative autoradiography in the hippocampus of 9 patients with Alzheimer's disease and 8 age-matched controls. Within the hippocampal formation from control subjects, [125I]apamin binding sites were highly concentrated in the subiculum and CA1. In Alzheimer's disease there was a marked and discrete loss of [125I]apamin binding sites in the subiculum (control = 1.10 +/- 0.10 pmol/g; Alzheimer = 0.71 +/- 0.09 pmol/g) and CA1 (control = 1.41 +/- 0.09 pmol/g; Alzheimer = 0.85 +/- 0.11 pmol/g; values are mean +/- S.E.M.). This reduction of [125I]apamin binding sites in the subiculum correlated with cell density but not neuritic plaque density. These results indicate that an anatomically discrete loss of Ca(2+)-dependent K+ channels within the hippocampal formation occurs in Alzheimer's disease.
Ikeda
M
M
Wellcome Surgical Institute, University of Glasgow, U.K.
Dewar
D
D
McCulloch
J
J
D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism*; D001030:Apamin / Q000378:metabolism*; D001345:Autoradiography; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D015221:Potassium Channels; D011712:Pyramidal Tracts / Q000378:metabolism; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D012016:Reference Values
D007457:Iodine Radioisotopes; D015221:Potassium Channels; D017981:Receptors, Neurotransmitter; C033799:apamin receptor; D001030:Apamin