One-electron oxidation of Trolox C and vitamin E by peroxidases.
Direct reactions of peroxidases with Trolox C (a vitamin E analogue) and vitamin E were observed in 50% (v/v) methanol. The kinetic results revealed that the reaction of horseradish peroxidase intermediate Compound II with Trolox C and vitamin E was the rate-determining step, and the rate constants were estimated to be 1.7 x 10(3) and 5.1 x 10(2) M-1.s-1, respectively. Peroxidases catalyzed the one-electron oxidation of Trolox C and vitamin E, and the vitamin E phenoxyl radicals resulting from the peroxidase reactions were detected by continuous-flow ESR spectroscopy.
Nakamura
M
M
Biophysics Division, Research Institute of Applied Electricity, Hokkaido University.
D000975:Antioxidants / Q000378:metabolism; D002839:Chromans / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D007784:Lactoperoxidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D014810:Vitamin E / Q000378:metabolism*
D000975:Antioxidants; D002839:Chromans; D007527:Isoenzymes; D014810:Vitamin E; D006735:Horseradish Peroxidase; D007784:Lactoperoxidase; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid