Copper-induced non-selective permeability changes in intracellularly perfused snail neurons.
The effect of extracellularly applied Cu2+ was studied on isolated intracellularly perfused Helix pomatia neurons. It was found that the Cu(2+)-activated current (ICu) is biphasic and composed of overlapping outward and inward components. The outward component of ICu is the result of a blockade by Cu2+ of the steady-state outward Cl- current. The inward component is assumed to flow through Ca(2+)-activated non-selective cationic channels. The washing-out procedure resulted in a large inward current (Iw), which was composed of transient and steady-state components. It is most likely that the activation of metabolic pumps is responsible for the transient component and the steady-state component is the result of increased neuronal membrane permeability for Cl-. Moreover, both ICu and Iw were highly Ca(2+)- and temperature-dependent processes. It is concluded that Cu2+ application resulted in complex permeability changes in the Helix pomatia neurons.
Kiss
T
T
Balaton Limnological Research Institute, Hungarian Academy of Sciences, Tihany.
Györi
J
J
Osipenko
O N
ON
Maginyan
S B
SB
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003300:Copper / Q000494:pharmacology*; D006372:Helix, Snails / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D010477:Perfusion; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects
D010042:Ouabain; D003300:Copper; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium