title: Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting.
abstract: Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.
authors.affiliation: Department of Veterinary Science, University of Nebraska, Lincoln 68583-0905.
authors.forename: C L
authors.initials: CL
authors.lastname: Kelling
authors.forename: J E
authors.initials: JE
authors.lastname: Kennedy
authors.forename: K K
authors.initials: KK
authors.lastname: Rump
authors.forename: L C
authors.initials: LC
authors.lastname: Stine
authors.forename: P S
authors.initials: PS
authors.lastname: Paul
authors.forename: J E
authors.initials: JE
authors.lastname: Partridge
mesh_terms: D000818:Animals; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005095:Exoribonucleases / Q000378:metabolism; D009710:Nucleotide Mapping; D009841:Oligonucleotides / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis* / Q000737:chemistry; D014765:Viral Vaccines / Q000032:analysis* / Q000235:genetics
chemical_list: D009841:Oligonucleotides; D012367:RNA, Viral; D014765:Viral Vaccines; D005095:Exoribonucleases; C042081:exoribonuclease T