title: Efficiency of various cloned DNA probes for detection of bovine viral diarrhea viruses.
abstract: We have evaluated 24 cytopathic (CP) and 37 noncytopathic (NCP) strains of bovine viral diarrhea virus (BVDV) with a dot blot assay using four different genome segments of the NADL strain as hybridization probes (p80, p54, gp53, and gp62). The p80 and p54 probes hybridized to 23/24 (96%) and 22/24 (92%), respectively, of CP strains examined. In contrast, these same two probes only detected 16/37 (43%) and 5/37 (13%), respectively, of the NCP strains examined. The gp53 probe detected 18/24 (75%) and the gp62 probe detected 19/24 (79%) of the CP strains. In contrast, these latter two probes only detected 9/37 (24%) and 7/37 (20%), respectively, of NCP strains. This low detection rate of NCP strains suggests a need for developing a probe based on NCP sequences for identification of NCP strains.
authors.affiliation: USDA, ARS, U.S. Mean Animal Research Center, Clay Center, NE 68933.
authors.forename: J
authors.initials: J
authors.lastname: Kwang
authors.forename: E T
authors.initials: ET
authors.lastname: Littledike
authors.forename: S
authors.initials: S
authors.lastname: Bolin
authors.forename: M S
authors.initials: MS
authors.lastname: Collett
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D004279:DNA, Viral