Human neutrophil responses to pathogenic Escherichia coli are receptor-specific and selectively augmented by recombinant human tumor necrosis factor-alpha.
The effect of recombinant human tumor necrosis factor-alpha (rhTNF alpha) on neutrophil (PMNL) response to uropathogenic Escherichia coli was assessed. A strain expressing mannose-sensitive adhesins (type 1 fimbriae) stimulated significant primary granule, secondary granule, and leukotriene B4 (LTB4) release. The same strain grown to suppress fimbrial expression and three non-type 1-fimbriated strains stimulated only background low-level PMNL activation. The binding of the type 1-fimbriated strain to PMNL was inhibitable by D-mannose and concanavalin A, while that of non-type 1-fimbriated strains was not but was inhibited by antibodies to the PMNL complement receptors 1 and 3. TNF alpha (10(-9) M) synergistically augmented the non-type 1-fimbriated E. coli-stimulated LTB4 release and additively increased secondary granule release without affecting primary granule release. In contrast, none of the responses to the type 1-fimbriated strain were augmented by TNF alpha. In the absence of mannose-sensitive adhesins, the activation of PMNL by E. coli may involve both complement receptors 1 and 3 and be augmented by TNF alpha.
Steadman
R
R
Institute of Nephrology, Kidney Research Unit Foundation, University of Wales College of Medicine, Cardiff Royal Infirmary, United Kingdom.
Matthews
N
N
Lichodziejewska
M
M
Williams
J D
JD
D001422:Bacterial Adhesion; D003208:Concanavalin A / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D004926:Escherichia coli / Q000276:immunology* / Q000472:pathogenicity / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology; D010861:Fimbriae, Bacterial / Q000276:immunology; D006801:Humans; D007975:Leukotriene B4 / Q000276:immunology; D008358:Mannose / Q000494:pharmacology; D008854:Microscopy, Electron; D009504:Neutrophils / Q000276:immunology* / Q000382:microbiology; D011951:Receptors, Complement / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*; D014552:Urinary Tract Infections / Q000382:microbiology
D011951:Receptors, Complement; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D007975:Leukotriene B4; D008358:Mannose