Interferon-gamma and immunoglobulin enhance mineral dust-induced production of reactive oxygen metabolites by human macrophages.
The modulating effect of interferon-gamma (IFN-gamma) and polyclonal immunoglobulin (poly-Ig) on mineral dust-induced production of reactive oxygen metabolites (ROM) by human monocyte-derived macrophages was investigated using lucigenin-dependent chemiluminescence. Activation of the cell cultures with IFN-gamma enhanced the subsequent quartz- and asbestos-induced production of ROM, while the addition of poly-Ig only stimulated chrysotile asbestos-induced ROM production. The combination of IFN activation and the addition of poly-Ig to the reactions caused a 20-fold enhancement of the responses to chrysotile. As increased levels of IFN-gamma and immunoglobulins have been observed in patients with mineral dust-induced diseases, we suggest that "immunological activation" caused by, for example, autoimmune diseases or recurrent pulmonary infections might amplify the production of potentially tissue-injuring ROM by mononuclear phagocytes and may possibly determine the individual susceptibility to mineral dust-related disorders.
Nyberg
P
P
Institute of Occupational Health, Helsinki, Finland.
Klockars
M
M
D001194:Asbestos / Q000009:adverse effects*; D017632:Asbestos, Serpentine; D004391:Dust / Q000009:adverse effects*; D006801:Humans; D007136:Immunoglobulins / Q000502:physiology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D011791:Quartz / Q000009:adverse effects*
D017632:Asbestos, Serpentine; D004391:Dust; D007136:Immunoglobulins; D001194:Asbestos; D011791:Quartz; D007371:Interferon-gamma; D010100:Oxygen