Kinetic and cross-linking studies indicate different receptors for endothelins and sarafotoxins in the ileum and cerebellum.
Kinetics of ligand/receptor interactions using ET-1, ET-3 and SRTX-b were studied and cross-linking experiments carried out in guinea pig ileum and rat cerebellar preparations. Dissociation studies indicate that the two regions are characterized by different receptor subtypes and different modes of ligand binding. Autoradiographic patterns obtained following cross-linking of ET-1 and ET-3 to the different tissues support these conclusions.
Galron
R
R
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
Bdolah
A
A
Kochva
E
E
Wollberg
Z
Z
Kloog
Y
Y
Sokolovsky
M
M
D000818:Animals; D001345:Autoradiography; D002531:Cerebellum / Q000378:metabolism*; D003432:Cross-Linking Reagents; D016232:Endothelins / Q000378:metabolism*; D007082:Ileum / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000378:metabolism*; D017466:Receptors, Endothelin; D018000:Receptors, Peptide
D003432:Cross-Linking Reagents; D016232:Endothelins; D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic; D017466:Receptors, Endothelin; D018000:Receptors, Peptide; C058085:sarafotoxin receptor