Removal of the 2.2.2 cryptand (Kryptofix 2.2.2) from 18FDG by cation exchange.
The 2.2.2 cryptand [4,7,13,16,21,24-hexaoxa-1,10-diazabicyclo-(8.8.8)-hexacosane] was trapped efficiently on Dowex AG50W-X8 (100-200 mesh) cation exchange resin. The concentration of the 2.2.2 cryptand in water with 0.1-2% methanol, or in 1 N HCl, was decreased by a factor of > 4000 using 1 mL (1.7 mequiv.) of AG50W-X8 and a flow rate of approx. 2 mL/min. K+, Cs+, Ag+ and Ba2+ forms were about equal in their ability to remove the 2.2.2 cryptand. A disposable cartridge containing 1 mL of hydrogen form resin was inserted into our automated 18FDG system so that the hydrolysate (in 2 cm3 of 1 N HCl) would pass through the cartridge before final purification. No cryptand was detected in the final product as determined by TLC with idoplantinate visualization. The detection limit was 2.5 micrograms/mL. Less than 3% of the total starting radioactivity was retained by the cation column. Quality assurance tests including apyrogenicity, sterility, radiochemical purity, carbohydrate composition and pH were not compromised by the incorporation of the cryptand removal cartridge.
Alexoff
D L
DL
Department of Chemistry, Brookhaven National Laboratory, Upton, NY 11973.
Fowler
J S
JS
Gatley
S J
SJ
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002411:Cation Exchange Resins; D002614:Chelating Agents; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000302:isolation & purification; D019788:Fluorodeoxyglucose F18
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002411:Cation Exchange Resins; D002614:Chelating Agents; D019788:Fluorodeoxyglucose F18; C006071:cryptating agent 222; D003847:Deoxyglucose