Receptor-mediated activation of arachidonic acid release in mouse peritoneal macrophages is linked to extracellular calcium influx.
The role of external calcium in platelet-activating factor- and zymosan-stimulated arachidonic acid release from mouse macrophages was investigated. Deprivation of external Ca2+ led to strong inhibition of receptor-mediated arachidonic acid release, which was completely restored when Ca2+ was added to the incubation medium. When arachidonic acid release was examined in Ca(2+)-depleted cells, the response took place only in presence of external Ca2+. Verapamil, a voltage-dependent Ca2+ channel blocker, nearly abolished arachidonic acid release in response to both platelet-activating factor and zymosan. These results suggest that extracellular Ca2+ influx is functionally linked to arachidonic acid release and hence to phospholipase A2 activation in mouse peritoneal macrophages.
Fernández
B
B
Centro de Investigación del Hospital Doce de Octubre, Madrid, Spain.
Balsinde
J
J
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D004533:Egtazic Acid / Q000494:pharmacology; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000502:physiology; D014700:Verapamil / Q000494:pharmacology*; D015054:Zymosan / Q000494:pharmacology
D010972:Platelet Activating Factor; D011956:Receptors, Cell Surface; D016718:Arachidonic Acid; D004533:Egtazic Acid; D015054:Zymosan; D014700:Verapamil; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium