Cyclic adenosine monophosphate-phosphodiesterase activity in cultured keratinocytes from patients with atopic eczema.
A number of previous studies have documented an increase in cyclic adenosine monophosphate-phosphodiesterase activity in patients with atopic dermatitis. We have studied the activity of this enzyme in keratinocytes cultured from patients with atopic dermatitis compared to keratinocytes from non-atopic controls. Results show that these pure preparations of keratinocytes express only one form of cyclic adenosine monophosphate phosphodiesterase with a Km of 1.9 microM, and a Vmax of 17 pmol/min/mg of substrate converted. We conclude that cyclic adenosine monophosphate-phosphodiesterase activity in cultured keratinocytes from patients with atopic eczema does not differ from controls.
Wright
S
S
Department of Experimental Dermatology, London Hospital, U.K.
Navsaria
H
H
Leigh
I M
IM
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D002478:Cells, Cultured; D003876:Dermatitis, Atopic / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology* / Q000473:pathology
D015105:3',5'-Cyclic-AMP Phosphodiesterases