title: Implication of human papillomavirus in postirradiation dysplasia.
abstract: The presence of human papillomavirus (HPV) DNA and association of condylomata acuminata (CA) in the biopsy tissues of postirradiation dysplasia (PRD) of the cervix and/or vagina from 17 patients who previously had radiation therapy for malignancies of the uterine cervix, vagina, and endometrium were evaluated with DNA in situ hybridization. Eight of 17 patients (47.1%) had HPV DNA identified in the lesions of postirradiation dysplasia (PRD). Five of eight cases (62.5%) contained HPV DNA of more than one type. Type 16 HPV DNA (HPV-16) was the most frequently identified type. Several PRD lesions also contained HPV-6, HPV-18, HPV-31, and/or HPV-33 DNA. Eleven patients (64.7%) showed CA in the vicinity of PRD. In two cases, different types of HPV were found in the lesions of PRD and contiguous CA. The frequency of the cases containing HPV DNA, the types of HPV, and the distribution pattern of silver grains in the preparations of in situ hybridization over the nuclei of cells of PRD were very similar to those found in naturally occurring dysplasia. Based on these findings, persistent or repeat HPV infection was the most likely etiologic factor of PRD, which might be facilitated by immunosuppression due to pelvic irradiation.
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis.
authors.forename: M
authors.initials: M
authors.lastname: Fujimura
authors.forename: R S
authors.initials: RS
authors.lastname: Ostrow
authors.forename: T
authors.initials: T
authors.lastname: Okagaki
mesh_terms: D000328:Adult; D000368:Aged; D003218:Condylomata Acuminata / Q000209:etiology / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005833:Genital Neoplasms, Female / Q000532:radiotherapy; D006801:Humans; D008875:Middle Aged; D009381:Neoplasms, Radiation-Induced / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D002578:Uterine Cervical Dysplasia / Q000209:etiology / Q000382:microbiology*; D014625:Vaginal Neoplasms / Q000209:etiology / Q000382:microbiology*
chemical_list: D004279:DNA, Viral