title: Constant expression and activity of protein phosphatase 2A in synchronized cells.
abstract: The levels of the A, B, and C subunits of protein phosphatase 2A in extracts from synchronized embryonic bovine tracheal cells were determined by immunoblotting with subunit-specific antibodies. A constant amount of each subunit was found in resting cells as well as in growing cells from all stages of the cell cycle. The phosphatase activity of protein phosphatase 2A was also constant. A quantitative comparison showed that the A and C subunits were present in similar amounts, whereas the B subunit was present at a significantly lower level. Together, the A, B, and C subunits represented approximately 0.2% of the total cellular protein.
authors.affiliation: Department of Pathology, University of California, San Diego, La Jolla 92093-0612.
authors.forename: R
authors.initials: R
authors.lastname: Ruediger
authors.forename: J E
authors.initials: JE
authors.lastname: Van Wart Hood
authors.forename: M
authors.initials: M
authors.lastname: Mumby
authors.forename: G
authors.initials: G
authors.lastname: Walter
mesh_terms: D000818:Animals; D002417:Cattle; D002453:Cell Cycle; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D007700:Kinetics; D046911:Macromolecular Substances; D009130:Muscle, Smooth / Q000166:cytology / Q000201:enzymology; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D054648:Protein Phosphatase 2; D014132:Trachea / Q000166:cytology / Q000201:enzymology
chemical_list: D046911:Macromolecular Substances; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2