Bovine lens capsule basement membrane collagen exerts a negative priming on polymorphonuclear neutrophils.
After a 30 min contact between purified bovine lens capsule basement membrane type IV collagen and polymorphonuclear neutrophils, stimulation of these cells by N-formyl-methionyl-leucyl-phenylalanine, PMA or type I collagen releases a decreased amount of superoxide ions (negative priming). The inhibitory activity is located in the NCl domain. On the other hand, after pepsin digestion, the helical part of type IV collagen determines a positive priming of neutrophils.
Monboisse
J C
JC
Laboratoire de Biochimie, URA CNRS 610, Faculté de Médecine de l'Université de Reims, France.
Bellon
G
G
Perreau
C
C
Garnotel
R
R
Borel
J P
JP
D000818:Animals; D001485:Basement Membrane; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D003094:Collagen / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D007908:Lens, Crystalline; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D013481:Superoxides / Q000097:blood; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003094:Collagen; D013755:Tetradecanoylphorbol Acetate