Subcellular localization of blood group A substance produced by pancreatic adenocarcinoma induced in hamsters by N-nitrosobis(2-oxopropyl)amine (BOP) and by its cell line (PC-1).
The subcellular localization and biochemical characteristics of blood group A antigen were studied by immunogold methods and by SDS-PAGE and Western blotting procedures in N-nitrosobis)2-oxopropyl)amine (BOP)-induced pancreatic cancer (PC) in Syrian hamsters, in the pancreatic cancer cell line (PC-1) derived from a primary induced pancreatic cancer, and in intrapancreatic and subcutaneous transplants of PC-1 cells. Normal hamster duodenal epithelial cells expressing A antigen were compared with the normal hamster pancreas (lacking A antigen), human PC tissues from patients with blood group A and human PC cell lines. Blood group A antigen was present on the membrane of hamster duodenal cells, but was absent in the normal pancreatic cells. A antigen was localized mainly on the cell membrane of the hamster cancer cells both in vivo and in vitro. Glycoproteins with blood group A specificity were observed by SDS-PAGE and Western blotting procedures in the membrane fraction of PC-1 cells, with a major component of molecular mass of approximately 120 kd. Similar migration patterns were observed in the primary induced PC and in subcutaneous and intrapancreatic transplants of PC-1 cells. Membrane preparations from cell lines derived from two primary pancreatic cancers from patients of blood group A and from human pancreatic cell lines, CD11 and CD18, showed a major A reactive component with a molecular mass similar to that found in the hamster PC cells. These findings suggest that: (i) both the hamster and human PC cells in vitro produce glycoproteins with blood group A specificity of similar molecular masses; (ii) differences exist in the structure of the glycoprotein immunoreactive with the anti-A antigen between the normal and cancerous cells; and (iii) differences exist in the molecular mass of the anti-A reactive substance between hamsters and human PC cells and between tissues in vivo and in vitro.
Egami
H
H
Eppley Institute for Research in Cancer and Allied Diseases, Omaha, NE.
Chaney
W G
WG
Takiyama
Y
Y
Pour
P M
PM
D000017:ABO Blood-Group System; D000230:Adenocarcinoma / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000943:Antigens, Differentiation / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D018845:CD11 Antigens; D018821:CD18 Antigens; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D006224:Cricetinae; D004386:Duodenum / Q000276:immunology / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D007279:Injections, Subcutaneous; D008854:Microscopy, Electron / Q000379:methods; D009368:Neoplasm Transplantation; D009602:Nitrosamines; D010179:Pancreas / Q000276:immunology / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D013347:Subcellular Fractions / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018845:CD11 Antigens; D018821:CD18 Antigens; D009602:Nitrosamines; D016028:Receptors, Leukocyte-Adhesion; D012967:Sodium Dodecyl Sulfate; C013622:nitrosobis(2-oxopropyl)amine