Nerve stimulation augments angiotensin II overflow from canine gracilis muscle in vivo.
The overflow of endogenous angiotensin-(1-8)octapeptide (angiotensin II) from blood-perfused canine gracilis muscle vasculature in situ was studied. A positive veno-arterial concentration difference for angiotensin II over the gracilis muscle was found, indicating a net generation of angiotensin II under basal conditions. Angiotensin II levels in the venous effluent were elevated during 2 Hz (4-min) nerve stimulation, suggesting enhanced local angiotensin II generation both in the presence and absence of alpha-adrenoceptor blockade. Thus, our results in this in vivo model demonstrate a local overflow of angiotensin II from the skeletal muscle vasculature which can be enhanced by nerve stimulation. Whether this overflow of angiotensin II is due to conversion of circulating angiotensin I to angiotensin II or local de novo synthesis of angiotensin II remains to be established.
Schwieler
J H
JH
Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Nussberger
J
J
Kahan
T
T
Hjemdahl
P
P
D000804:Angiotensin II / Q000378:metabolism*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D004285:Dogs; D004558:Electric Stimulation; D005260:Female; D009132:Muscles / Q000378:metabolism*; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects; D012084:Renin-Angiotensin System / Q000502:physiology; D013564:Sympathetic Nervous System / Q000502:physiology*
D000806:Angiotensin-Converting Enzyme Inhibitors; D001552:Benzazepines; D011942:Receptors, Adrenergic, alpha; D000804:Angiotensin II; C052133:benazeprilat