Hydralazine stimulates production of oxygen free radicals in Eagle's medium and cultured fibroblasts.
The effect of hydralazine on the oxygen free radical production was studied in whole cultured murine liver fibroblasts and mitochondrial and microsomal fractions of the cells by ESR spin trapping with DMPO and measurement of Tiron semiquinone formation. Hydralazine itself was found to generate free radicals in phosphate buffer and especially in Eagle's Minimal Essential Medium. Most of the adduct of the spin trap DMPO was due to its reaction with hydralazine-induced hydroxyl radical. Moreover, this compound stimulated free radical formation in fibroblasts. These data suggest that hydralazine alters the cellular free radical metabolism which may have implications for the biological activity of this drug.
Weglarz
L
L
Department of Biochemistry and Chemistry, Silesian Medical Academy, Katowice, Poland.
Bartosz
G
G
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005609:Free Radicals; D006830:Hydralazine / Q000494:pharmacology*; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*
D003470:Culture Media; D005609:Free Radicals; D006830:Hydralazine; D010100:Oxygen