Identification of Thy-1 homologue in rabbit brain by immunoblot, immunohistochemistry and DNA/DNA hybridization.
A membrane glycoprotein with an apparent molecular weight of approximately 26,000 reacted on immunoblot with a monoclonal antibody (HB-3S-17) directed toward human Thy-1. At cellular level, HB-3S-17 reacted with both rabbit and human cerebral cortexes in a similar manner as demonstrated by immunohistochemical staining. Screening of a rabbit brain expression cDNA library with HB-3S-17 resulted in the isolation of a clone designated RBT-2A-1. The rabbit cDNA insert of RBT-2A-1 hybridized in Southern blot with an oligonucleotide probe derived from the mouse Thy-1.2 gene. These data strongly indicate the existence of a glycoprotein in rabbit brain which is the counterpart of human and mouse Thy-1.
Wang
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AC
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston 29425.
Chao
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CC
Arnaud
P
P
Wang
I Y
IY
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D001921:Brain / Q000276:immunology*; D004247:DNA / Q000032:analysis; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D009693:Nucleic Acid Hybridization; D011817:Rabbits; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D018800:Thy-1 Antigens; D004247:DNA