title: Involvements of fibronectin and lysophosphatidylcholine for selective binding of C-reactive protein.
abstract: C-reactive protein (CRP) has been reported to deposit only to inflammatory sites, but not to normal sites. In present paper, we investigated involvements of fibronectin and lysophosphatidylcholine (lyso-PC) as responsible for this selectivity. In ELISA assay, CRP was found to bind to immobilized fibronectin with dose dependency, only in the presence of Ca2+ ions. Addition of 5 mM EDTA allowed CRP to abolish this binding. However, it could not be inhibited neither by phosphorylcholine nor by heparin. On the other hand, CRP could aggregate liposome consisted of lyso-PC and phosphatidylcholine (PC), but not that consisted of PC alone. Aggregation was found to be maximum when liposome with lyso-PC/PC molar ratio of 0.3 was used. Similar result was also observed in binding study with peroxidase-labelled CRP. In addition, phospholipase A2 treatment of liposome consisted of PC alone induced 3-fold higher binding than that found with untreated one. Ca2+ ions were required for binding to liposome.
authors.affiliation: School of Health Sciences, Okayama University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Nakata
authors.forename: H
authors.initials: H
authors.lastname: Endo
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D010767:Phosphorylcholine / Q000378:metabolism; D011817:Rabbits
chemical_list: D005353:Fibronectins; D008081:Liposomes; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010767:Phosphorylcholine; D006493:Heparin; D002097:C-Reactive Protein; D009195:Peroxidase; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium