Cytomegalovirus infection of human kidney cells in vitro.
To study which structures of a kidney allograft are the main targets for cytomegalovirus (CMV), human glomerular epithelial and mesangial cells, as well as tubular epithelial and endothelial cells were isolated by steel meshes of different pore sizes and enzymatic treatments. The various cultured cell types were characterized by morphology and specific antibodies. Human CMV was inoculated onto cell monolayers using two different culture methods: conventional tissue culture and rapid shell vial culture. To analyze whether CMV had a direct effect on the immunologic properties of kidney parenchymal cells, MHC class I and class II antigen expression was estimated before and after the infection. CMV infected all kidney cells identically. All cells expressed class I strongly after the infection, but they were class I positive prior to infection. Class II antigens were not expressed on the cell surface either before or after the infection. In conclusion, human kidney cells of glomerular, tubular and vascular origin were all infected by CMV without any difference. CMV had no significant direct effects on the antigenic properties of the cells.
Ustinov
J A
JA
Transplantation Laboratory, Fourth Department of Surgery, University of Helsinki, Finland.
Loginov
R J
RJ
Mattila
P M
PM
Nieminen
V K
VK
Suni
J I
JI
Häyry
P J
PJ
Lautenschlager
I T
IT
D000328:Adult; D003586:Cytomegalovirus Infections / Q000209:etiology*; D004730:Endothelium, Vascular / Q000382:microbiology / Q000473:pathology; D004848:Epithelium / Q000382:microbiology / Q000473:pathology; D005920:Glomerular Mesangium / Q000382:microbiology / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology / Q000382:microbiology*; D007674:Kidney Diseases / Q000209:etiology / Q000382:microbiology / Q000473:pathology; D007678:Kidney Glomerulus / Q000382:microbiology / Q000473:pathology; D016030:Kidney Transplantation / Q000009:adverse effects*; D007684:Kidney Tubules / Q000382:microbiology / Q000473:pathology