Introduction and transposition of the maize transposable element Ac in rice (Oryza sativa L.).
To develop a transposon tagging system in an important cereal plant, rice (Oryza sativa L.), the maize transposable element Ac (Activator) was introduced into rice protoplasts by electroporation. We employed a phenotypic assay for excision of Ac from the selectable hph gene encoding resistance to hygromycin B. Southern blot analysis of hygromycin B-resistant calli showed that the Ac element can transpose from the introduced hph gene into the rice chromosomes. Sequence analysis of several Ac excision sites in the hph genes revealed sequence alterations characteristic of the excision sites of this plant transposable element. The Ac element appears to be active during development of transgenic rice plants from calli. Moreover, hybridization patterns of different leaves from the same plant indicated that some Ac elements are stable whereas others are able to transpose further during development of leaves. The results indicate that the introduced Ac element can transpose efficiently in transgenic rice plants.
Izawa
T
T
Plantech Research Institute, Yokohama, Japan.
Miyazaki
C
C
Yamamoto
M
M
Terada
R
R
Iida
S
S
Shimamoto
K
K
D001483:Base Sequence; D015139:Blotting, Southern; D004251:DNA Transposable Elements; D004560:Electricity; D006921:Hygromycin B / Q000494:pharmacology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping; D003313:Zea mays / Q000235:genetics*
D004251:DNA Transposable Elements; D006921:Hygromycin B