title: Raman spectroscopic characterization of tryptophan side chains in lysozyme bound to inhibitors: role of the hydrophobic box in the enzymatic function.
abstract: The state of H-bonding and the hydrophobic interaction of six tryptophan side chains in lysozyme bound to substrate-analogous inhibitors were investigated by combining H----D exchange labeling and Raman difference spectroscopy. The frequency of the W17 band due to Trp-63 shifts downward upon inhibitor binding, indicating a specific and strong H-bond formation between the N1 site of the side chain and the inhibitor molecule. On the other hand, the H-bonding state of Trp-62 in the complex is as weak as that in inhibitor-free lysozyme, suggesting no contribution of this residue to the inhibitor binding. Intensity increases of W17 and W18 bands observed upon inhibitor binding are, respectively, ascribed to an increase at Trp-28 and a decrease at Trp-111 in hydrophobic interactions with the environment. The environmental changes are explained consistently by a movement of the Met-105 side chain sandwiched by two indole rings of Trp-28 and 111 in the direction from Trp-111 to Trp-28. The sandwich structure in a core domain, hydrophobic box, and its rearrangement are considered to play an important role in the enzymatic function of lysozyme.
authors.affiliation: Pharmaceutical Institute, Tohoku University, Sendai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miura
authors.forename: H
authors.initials: H
authors.lastname: Takeuchi
authors.forename: I
authors.initials: I
authors.lastname: Harada
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D003903:Deuterium; D017666:Deuterium Oxide; D005260:Female; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009113:Muramidase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D013059:Spectrum Analysis, Raman / Q000379:methods; D014364:Tryptophan; D014867:Water
chemical_list: D014867:Water; D014364:Tryptophan; D003903:Deuterium; D009113:Muramidase; D017666:Deuterium Oxide; D000117:Acetylglucosamine