title: Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization.
abstract: Two genes encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were localized in human and rat chromosomes. PFKFB1 (previously PFRX), which encodes the liver and muscle isozymes, was assigned to Xq22-q31 in the rat and to Xq27-q28 in the human by in situ hybridization using probes generated by the polymerase chain reaction. PFKFB2, which encodes the heart isozyme of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, was assigned to chromosome 13 in the rat and to chromosome 1 in the human by hybridization of DNA from somatic cell hybrids. By in situ hybridization, this gene was localized to the regions 13q24-25 in the rat and 1q31 in the human.
authors.affiliation: Center for Human Genetics, University of Leuven, Belgium.
authors.forename: C E
authors.initials: CE
authors.lastname: Hilliker
authors.forename: M I
authors.initials: MI
authors.lastname: Darville
authors.forename: M S
authors.initials: MS
authors.lastname: Aly
authors.forename: M
authors.initials: M
authors.lastname: Chikri
authors.forename: C
authors.initials: C
authors.lastname: Szpirer
authors.forename: P
authors.initials: P
authors.lastname: Marynen
authors.forename: G G
authors.initials: GG
authors.lastname: Rousseau
authors.forename: J J
authors.initials: JJ
authors.lastname: Cassiman
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D005091:Exons; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D010770:Phosphotransferases; D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases