Reduction of Li(+)-Na+ countertransport by physiological levels of insulin in vitro.
We studied the effect of insulin at concentrations of 0 to 50 mU/l and glucose at 0 to 20 mmol/l on Li(+)-Na+ countertransport in erythrocytes from normotensive human subjects. While insulin produced a dose-dependent decrease in the rate of Li(+)-Na+ countertransport, from 0.272 mmol/l erythrocytes per h at zero insulin to 0.081 at a concentration of 50 mU/l (P less than 0.0001), alterations in ambient glucose concentration had no significant effect on the rate of countertransport. Physiological levels of insulin appear to reduce Li(+)-Na+ countertransport in vitro by a mechanism which is not related to glucose concentration or transport.
Foyle
W J
WJ
Department of Diabetes, King's College Hospital, Denmark Hill, London, UK.
Drury
P L
PL
D000328:Adult; D017920:Antiporters; D001692:Biological Transport / Q000187:drug effects; D001786:Blood Glucose / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D012016:Reference Values
D017920:Antiporters; D001786:Blood Glucose; D002352:Carrier Proteins; D007328:Insulin; C045885:sodium-lithium countertransporter