Isolation and characterization of new restriction endonucleases from Vibrio parahaemolyticus: VpaK32I enzyme with the class-IIS heptanucleotide specificity, GCTCTTCN1/N4.
Six restriction endonucleases (ENases), classified into four different specificities, were found in a screen among 68 reference strains of Vibrio parahaemolyticus of human origin. Five of these ENases are isoschizomers of well-known ENases, while the remaining one, designated VpaK32I, is a novel and highly efficient class-IIS ENase with the hepatanucleotide recognition site, 5'-GCTCTTC(1/4)-3'.
Miyahara
M
M
Division of Microbiology, National Institute of Hygienic Sciences, Tokyo, Japan.
Shimada
T
T
Kotani
H
H
Mise
K
K
D001483:Base Sequence; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D014735:Vibrio Infections / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000201:enzymology* / Q000302:isolation & purification
D004269:DNA, Bacterial; C075962:endodeoxyribonuclease VpaK32I; D015252:Deoxyribonucleases, Type II Site-Specific