title: The effect of virus infection on the adherence of leukocytes or platelets to endothelial cells.
abstract: It has been reported that atherosclerotic lesions contain genomic material belonging to members of the herpes family. This suggests that latent viral infection may be one of the atherogenic triggers. In this study we show that early infection of endothelial cell monolayers with Herpes Simplex virus type 1 (HSV-1) or Cytomegalovirus (CMV) results in an increased monocyte (MC) and polymorphonuclear leukocyte (PMN) adherence, but not in an increased platelet adhesion. Further, is demonstrated that MC and PMN respond differently to virus infected endothelial cell monolayers: PMN adhesion to CMV infected cells is approximately 430% of the control adherence, while the MC adherence is increased to 160%. Also, a difference in virus acting is observed: the adherence of MC or PMN to HSV-1 infected endothelial cells is caused by a secreted adherence promoting factor, while the adherence of MC or PMN to CMV infected endothelial cells seems to be a cell-bound phenomenon. In addition, it was demonstrated that the augmentation of MC or PMN adherence to virus infected endothelial cells is sensitive to tunicamycin, suggesting that both virus infections induce the expression of glycoproteins on the endothelial cell membrane, which is responsible for the MC and PMN adhesion. Thus, HSV-1 and CMV infection of endothelium results in an increased adherence of leukocytes which is suggested, irrespective of the precise nature of the mechanism of virus induced atherosclerosis, to be the earliest event associated with endothelium cell damage.
authors.affiliation: Department of Medical Microbiology, University of Limburg, Maastricht, The Netherlands.
authors.forename: A H
authors.initials: AH
authors.lastname: Span
authors.forename: M C
authors.initials: MC
authors.lastname: van Dam-Mieras
authors.forename: W
authors.initials: W
authors.lastname: Mullers
authors.forename: J
authors.initials: J
authors.lastname: Endert
authors.forename: A D
authors.initials: AD
authors.lastname: Muller
authors.forename: C A
authors.initials: CA
authors.lastname: Bruggeman
mesh_terms: D001161:Arteriosclerosis / Q000209:etiology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003586:Cytomegalovirus Infections / Q000473:pathology; D004730:Endothelium, Vascular / Q000473:pathology; D006561:Herpes Simplex / Q000473:pathology; D006801:Humans; D007962:Leukocytes / Q000473:pathology*; D009000:Monocytes / Q000473:pathology; D009504:Neutrophils / Q000473:pathology; D010973:Platelet Adhesiveness / Q000187:drug effects; D014415:Tunicamycin / Q000494:pharmacology; D014777:Virus Diseases / Q000473:pathology*
chemical_list: D014415:Tunicamycin