Identification by biotinylated DNA probes of Capnocytophaga species isolated from supragingival calculus.
The distribution, genetic relatedness, and peptidase activity in hydrolyzing N-benzoyl-L-arginine-p-nitroanilide by Capnocytophaga species isolated from calculus- and non-calculus-formers were determined in this study. The proportion and prevalence of Capnocytophaga species were higher in plaque samples from the calculus group than in those from the non-calculus group, and these bacteria showed high peptidase activity. C. gingivalis were found more frequently in the calculus group than in the non-calculus group, and possessed much higher peptidase activity than C. ochracea and C. sputigena. Black-pigmented colonies were also recovered more frequently from the calculus group than from the non-calculus group. Since these organisms had little or no peptidase activity, they were not studied further.
Morita
M
M
Department of Preventive Dentistry, Okayama University Dental School, Japan.
Yamamoto
T
T
Watanabe
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D001120:Arginine / Q000031:analogs & derivatives; D001586:Benzoylarginine Nitroanilide; D001710:Biotin; D002206:Capnocytophaga / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D015169:Colony Count, Microbial; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D003728:Dental Calculus / Q000201:enzymology / Q000209:etiology / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology; D010450:Endopeptidases / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004269:DNA, Bacterial; D001710:Biotin; D001586:Benzoylarginine Nitroanilide; D001120:Arginine; D010450:Endopeptidases