Branched DNA amplification multimers for the sensitive, direct detection of human hepatitis viruses.
Branched oligonucleotides (bDNA) have been synthesized containing a unique primary segment and a set of identical secondary fragments covalently attached to the primary sequence through branch points. The primary sequence is designed to hybridize (directly or indirectly) to a target nucleic acid, such as hepatitis B virus (HBV) or hepatitis C virus (HCV) genomic DNA or RNA, respectively. The secondary fragments are used to direct the binding of multiple copies of a small oligonucleotide labelled with alkaline phosphatase. Assays for the presence of HBV and HCV based on the application of these branched amplification multimers have been devised. It is possible to detect as few as 1,000 hepatitis viral genomes directly.
Urdea
M S
MS
Chiron Corporation, Emeryville, CA 94608.
Horn
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T
Fultz
T J
TJ
Anderson
M
M
Running
J A
JA
Hamren
S
S
Ahle
D
D
Chang
C A
CA
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D009838:Oligodeoxyribonucleotides
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides