title: Differences in the induction of urate deposition of specific-pathogen-free chicks inoculated with avian nephritis virus passaged by five different methods.
abstract: The G-4260 strain of avian nephritis virus (ANV) was passaged using five different methods as follows: method 1, passage three times in chorioallantoic membrane (CAM) of 11-day-old embryonated eggs (CAM3); method 2, passage twice in CAM and further passage once in yolk sac (YS) of 6-day-old embryonated eggs (CAM2-YS1); method 3, passage 11 times in CAM (CAM11); method 4, passage 10 times in CAM and further passage twice in YS (CAM10-YS2); method 5, passage as in Method 4 and then passage three times in chicken kidney cell culture (CAM10-YS2-CK3). CAM11 and CAM10-YS2 were each inoculated orally into 25 one-day-old specific-pathogen-free (SPF) chicks. Seven chicks in the CAM11-inoculated group and six chicks in the CAM10-YS2-inoculated group died or were killed because they were moribund; all had either nephrosis or urate deposition. CAM3, CAM2-YS1, CAM10-YS2, and CAM10-YS2-CK3 were each inoculated intraperitoneally into 15 one-day-old SPF chicks. No chicks inoculated with CAM3 or CAM2-YS1 died, but wo chicks inoculated with CAM10-YS2 and three inoculated with CAM10-YS2-CK3 died with urate deposition. At 14 postinoculation, plasma urate values of the CAM10-YS2- and CAM10-YS2-CK3-inoculated chicks were significantly higher than those of CAM3- and CAM2-YS1-inoculated chicks and control chicks (P less than 0.01). However, interstitial nephritis was observed in most of the ANV-inoculated birds.
authors.affiliation: Poultry Disease Laboratory, National Institute of Animal Health, Gifu, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Shirai
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Nozaki
authors.forename: H
authors.initials: H
authors.lastname: Kawamura
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D007668:Kidney / Q000737:chemistry / Q000382:microbiology; D009395:Nephritis, Interstitial / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D009401:Nephrosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D010849:Picornaviridae / Q000472:pathogenicity*; D010850:Picornaviridae Infections / Q000097:blood / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D011201:Poultry Diseases / Q000097:blood / Q000382:microbiology* / Q000473:pathology; D012692:Serial Passage; D013047:Specific Pathogen-Free Organisms; D014527:Uric Acid / Q000032:analysis* / Q000097:blood
chemical_list: D014527:Uric Acid