Involvement of glycoprotein C (gC) in adsorption of herpes simplex virus type 1 (HSV-1) to the cell.
Results demonstrating involvement of glycoprotein C (gC) of herpes simplex type 1 virus (HSV-1) in attachment of the virus to the cell are presented. Monoclonal antibodies against gC-1 inhibited adsorption of gC(+)-strains. The gC(-)-mutant, MP, attached to cells but at a reduced rate. Attachment of the MP-mutant was unaffected by presence of anti-gC-1 antibody. Purified truncated gC-1 adsorbed to cells at a rate essentially the same as that of gC(+)-virus. Glycoprotein C-1 pretreated with heparin did not adsorb to cells. The results are compatible with a suggested role for gC in HSV attachment.
Svennerholm
B
B
Department of Clinical Virology, University of Göteborg, Göteborg, Sweden.
Jeansson
S
S
Vahlne
A
A
Lycke
E
E
D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D006493:Heparin / Q000494:pharmacology; D009154:Mutation; D011991:Receptors, Virus / Q000378:metabolism*; D018139:Simplexvirus / Q000235:genetics / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D011991:Receptors, Virus; D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; D006493:Heparin