Morphology of neurites from N18TG2 cell induced by protein kinase inhibitor H-7 and by cAMP.
Protein kinase inhibitor H-7 and dibutyryl (dB)-cAMP were found to induce neuritic processes in mouse neuroblastoma N18TG2 cells (36). In the present study, morphological differences between the neurites induced by H-7 and those by dB-cAMP were examined using electron microscopy (TEM and SEM) and tubulin immunohistochemistry. It was observed that: 1) The neurites induced by H-7 were relatively thin and frequently had varicosities. On the other hand, the neurites induced by dB-cAMP were thick but they had few varicosities. 2) Centrioles were frequently observed in the cells treated with dB-cAMP but were not encountered in the H-7-treated cells. 3) TEM and tubulin immunohistochemistry revealed that the main shafts of the neurites induced either by H-7 or dB-cAMP were filled with microtubules, but that the varicosities induced by H-7 contained a smaller amount of microtubules. 4) The stability to colchicine was greater in the neurites induced by H-7 than in those by dB-cAMP. From these features of the neurites, it was inferred that neurite outgrowth induced by dB-cAMP is deeply related to the formation of microtubules and that the neurites induced by H-7 were involved in other processes probably including an adhesive property of cell surfaces.
Ono
K
K
Third Department of Anatomy, Okayama University Medical School, Japan.
Katayama
N
N
Yamagata
Y
Y
Tokunaga
A
A
Tsuda
M
M
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000648:ultrastructure*; D003994:Bucladesine / Q000494:pharmacology*; D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D007546:Isoquinolines / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure; D009447:Neuroblastoma; D010879:Piperazines / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors
D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D003994:Bucladesine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000242:Cyclic AMP; D003078:Colchicine