title: Differential distribution of (Na,K)-ATPase alpha isoform mRNAs in the peripheral nervous system.
abstract: mRNA transcripts for 3 isoforms of the alpha subunit of (Na,K)-ATPase have been previously identified in the nervous system (designated alpha 1, alpha 2, and alpha 3). In order to study the localization and expression of the different alpha isoforms in the peripheral nervous system, we prepared probes from the unique 3' untranslated region of alpha 1 cDNA, and from the translated region of alpha 3 cDNA. These probes were used in dot blot and in situ hybridization assays of rat spinal cord, dorsal root ganglia (DRG), and sciatic nerve. Within the ventral horn of lumbar spinal cord, alpha 1 mRNA was found in a discrete set of laterally placed motor neurons, while alpha 3 was found in all the identified neurons of the spinal cord, including those motor neurons containing alpha 1. In the lumbar DRG, alpha 3 was uniformly distributed in DRG neurons, while alpha 1 was abundant in some neurons but little or none was found in other neurons. Satellite cells contained neither isoform. Schwann cells in sciatic nerve were labeled with the alpha 1 probe in a perinuclear distribution, but contained no detectable alpha 3. Dot blot analysis showed alpha 1 and alpha 3 in spinal cord and DRG, but only alpha 1 in peripheral nerve.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: GRECC Neurology Research Laboratory, VA Medical Center, Ann Arbor, MI.
authors.forename: M
authors.initials: M
authors.lastname: Mata
authors.forename: G J
authors.initials: GJ
authors.lastname: Siegel
authors.forename: V
authors.initials: V
authors.lastname: Hieber
authors.forename: M W
authors.initials: MW
authors.lastname: Beaty
authors.forename: D J
authors.initials: DJ
authors.lastname: Fink
mesh_terms: D000818:Animals; D005727:Ganglia, Spinal / Q000378:metabolism; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis; D009046:Motor Neurons / Q000201:enzymology; D009475:Neurons, Afferent / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D010525:Peripheral Nerves / Q000201:enzymology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D012583:Schwann Cells / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D013462:Sulfur Radioisotopes
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D000254:Sodium-Potassium-Exchanging ATPase