Detection of genital papillomavirus types by polymerase chain reaction using common primers.
We describe the detection of eight genital human papillomavirus (HPV) types, including HPV16 and HPV18, by PCR amplification of a 323 base-pair region of the genome within the L1 open reading frame (ORF). The primer sequences are: TGYAAATATCCWGATTWTWT and GTATCWACMACAGTAACAAA. The method will detect purified HPV16 DNA down to a concentration of as little as a single molecule in 100 microliters. The method is also applicable to purified DNA and crude lysates from tumour biopsies. Typing of the PCR product can be achieved with specific oligonucleotide probes.
Jenkins
A
A
A/S Telelab, Skien, Norway.
Kristiansen
B E
BE
Ask
E
E
Oskarsen
B
B
Kristiansen
E
E
Lindqvist
B
B
Trope
C
C
Kjørstad
K
K
D001483:Base Sequence; D015139:Blotting, Southern; D003218:Condylomata Acuminata / Q000382:microbiology*; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010761:Phosphorus Radioisotopes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D004279:DNA, Viral; D015345:Oligonucleotide Probes; D010761:Phosphorus Radioisotopes