Effects of calcium-phosphate-based materials on proliferation and alkaline phosphatase activity of newborn rat periosteal cells in vitro.
The effects of dental materials, intended for bone substitution, on cell growth and alkaline phosphatase activity of newborn rat periosteal cells have been studied in vitro. Confluent periosteal cells were exposed to three apatite-based materials (400 micrograms/mL) with different physico-chemical properties. The materials were a beta-tricalcium phosphate with a microporous granular structure obtained by sinterization (Synthograft, Johnson & Johnson, East Windsor, NY), a 40-60-mesh microporous durapatite ceramic (Periograf, Sterling Drug, Inc., Rensselaer, NY), and a 1-2-mm-diameter hydroxyapatite ceramic (Osprovit, Feldmuhle Aktiengeselschaft, Plochingen, Germany) with macropores larger than 100 microns. Cell proliferation and alkaline phosphatase activity were assessed by incorporation of 3H-thymidine into trichloroacetic-acid-precipitable material and by a fluorimetric method, respectively. Cell viability and compatibility with the materials were determined by morphology in phase-contrast microscopy. Periosteal cells showed increased proliferation following exposure to Synthograft, but were unaffected by Osprovit, whereas Periograf caused significantly reduced cell growth. Alkaline phosphatase activity was unaffected by Osprovit, but was decreased by both Synthograft and Periograf. The results indicated a differential response of periosteal cells to bone-substituting materials with heterogeneous physico-chemical characteristics.
Teti
A
A
Institute of Human Anatomy, University of Bari, Italy.
Tarquilio
A
A
Grano
M
M
Colucci
S
S
Laforgia
A
A
Mangini
F
F
Zambonin Zallone
A
A
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry* / Q000494:pharmacology; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D000242:Cyclic AMP / Q000032:analysis; D017886:Durapatite; D006882:Hydroxyapatites / Q000737:chemistry* / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D015675:Osteocalcin / Q000032:analysis; D010521:Periosteum / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D051381:Rats
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D006882:Hydroxyapatites; C485817:beta-tricalcium phosphate; D015675:Osteocalcin; D003094:Collagen; D017886:Durapatite; D000242:Cyclic AMP; D000469:Alkaline Phosphatase; C018392:tricalcium phosphate