Characterization of the DNA polymerase gene of human herpesvirus 6.
The construction of a recombinant bacteriophage lambda library containing overlapping clones covering 155 kbp of the 161-kbp genome of the Ugandan U1102 isolate of human herpesvirus 6 (HHV-6) is described. The use of degenerate-primer polymerase chain reaction allowed the isolation of a DNA probe for the DNA polymerase gene of HHV-6, which was subsequently used to isolate and position the pol gene on the physical map of the viral genome. A 4.4-kbp EcoRI DNA restriction fragment containing the pol gene was isolated and sequenced. The open reading frames flanking the pol gene code for the HHV-6 glycoprotein B gene and the human cytomegalovirus UL53 homolog. This arrangement is different from that seen in the alpha and gamma herpesvirus families, lending further support to the notion that HHV-6 is a member of the beta herpesvirus group.
Teo
I A
IA
Department of Virology, Royal Postgraduate Medical School, London, England.
Griffin
B E
BE
Jones
M D
MD
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003062:Codon; D004279:DNA, Viral / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D005814:Genes, Viral; D015698:Genomic Library; D015654:Herpesvirus 6, Human / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D015678:Viral Structural Proteins / Q000235:genetics*
D003062:Codon; D004279:DNA, Viral; D015678:Viral Structural Proteins; D004259:DNA-Directed DNA Polymerase