[3H]hemicholinium-3 binding in rats with status epilepticus induced by lithium chloride and pilocarpine.
The specific binding of [3H]hemicholinium-3 ([3H]HCh-3) and high-affinity [3H]choline uptake were measured in rats with status epilepticus induced by lithium and pilocarpine. The specific binding of [3H]HCh-3 in cortex and hippocampus from rats with status epilepticus increased to 2- to 3-fold of control while the striatal [3H]HCh-3 binding increased minimally. Scatchard analyses revealed that the observed changes resulted from an increase in Bmax of [3H]HCh-3 binding. High-affinity [3H]choline uptake remained unchanged. These results further implicate phospholipase A2 in the regulation of [3H]HCh-3 binding sites.
Yamada
K
K
Department of Psychiatry, Neuroscience, Pharmacology and Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Saltarelli
M D
MD
Coyle
J T
JT
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D002712:Chlorides / Q000627:therapeutic use*; D002794:Choline / Q000378:metabolism; D006426:Hemicholinium 3 / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008094:Lithium / Q000627:therapeutic use*; D018021:Lithium Chloride; D008297:Male; D010862:Pilocarpine / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000139:chemically induced / Q000517:prevention & control; D013226:Status Epilepticus / Q000188:drug therapy / Q000378:metabolism*
D002712:Chlorides; D010862:Pilocarpine; D006426:Hemicholinium 3; D008094:Lithium; D018021:Lithium Chloride; D002794:Choline; D000109:Acetylcholine