Changes in the enzyme activity of the myocardium and participation of the alpha-adrenergic and M-cholinergic systems in experimental isoprenaline myocardial hypertrophy.
With a view to clarifying the mechanisms of haemodynamic stress, as well as the role of some neurotransmitter systems in the pathogenesis of myocardial hypertrophy (MH), a complex of experiments was carried out with an MH model induced in rats by isoprenaline treatment and by applying the M-cholinolytic agent atropine and the alpha-adrenergic blocker phentolamine in a part of the experimental groups. The changes in the activity of some enzymes of myocardial metabolism, namely: glucose-6-phosphate dehydrogenase, pyruvate kinase, alpha-hydroxy-butyrate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase and creatine phosphokinase, were traced in 33 male Wistar rats. Considerable activation of glucose-6-phosphate dehydrogenase was found in the different experimental groups, suggesting the adaptive "anti-stress" character of this change. The results of the pathobiochemical studies presuppose the substantial participation of the alpha-adrenergic and of the M-cholinergic systems in the stage of compensated isoprenaline-induced myocardial hypertrophy.
Kolarova
R
R
Research Medico-Biological Institute, Medical Academy, Sofia, Bulgaria.
Petkov
O
O
Popova
N
N
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D006332:Cardiomegaly / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D007202:Indicators and Reagents; D007545:Isoproterenol; D008297:Male; D009206:Myocardium / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000378:metabolism*
D007202:Indicators and Reagents; D011942:Receptors, Adrenergic, alpha; D011950:Receptors, Cholinergic; D001285:Atropine; D007545:Isoproterenol