Human immunodeficiency virus type 1 tat gene enhances human cytomegalovirus gene expression and viral replication.
Clonal lines of human rhabdomyosarcoma (RD) cells, constitutively expressing human immunodeficiency virus type 1 (HIV-1) tat gene (RD tat cell lines) showed enhanced expression of human cytomegalovirus (HCMV) immediate-early (IE) and late (L) proteins upon HCMV infection, as compared with control RD cells. One of the RD tat cell lines produced infectious HCMV. The RD-tat cell lines, following transfection with recombinant plasmids containing the full length of the HCMV-IE enhancer/promoter linked to the bacterial chloramphenicol acetyltransferase (CAT) gene, exhibited an increased CAT expression by the tat product. A chronically HIV-1-infected human T-lymphoid cell line, SupT1, superinfected with HCMV, expressed HCMV-IE proteins while the parental SupT1 cells infected with HCMV were negative. Parental SupT1 cells coinfected with HIV-1 and HCMV also expressed HCMV-IE proteins, indicating that HIV-1-encoded proteins exert a positive regulatory effect on HCMV expression.
Ho
W Z
WZ
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Ayyavoo
V
V
Srinivasan
A
A
Stinski
M F
MF
Plotkin
S A
SA
Gönczöl
E
E
D000956:Antigens, Viral / Q000096:biosynthesis / Q000276:immunology; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D004279:DNA, Viral; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D015756:Genes, tat / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000235:genetics
D000956:Antigens, Viral; D004279:DNA, Viral; D015500:Chloramphenicol O-Acetyltransferase