title: The chloroplast beta-subunit allows assembly of the Escherichia coli F0 portion of the energy transducing adenosine triphosphatase.
abstract: The effect of the expression of the chloroplast F1-ATPase beta-subunit in two Escherichia coli beta-subunit mutant strains was investigated. The amount of chloroplast beta-subunit formed in E. coli was increased by introducing a 'Shine-Dalgarno' sequence upstream from the translation start site. The chloroplast beta-subunit was membrane bound but was unable to functionally replace the mutant beta-subunit in a strain carrying the uncD409 allele [corrected]. However, in an E. coli mutant strain unable to form the beta- and epsilon-subunits the presence of the chloroplast beta-subunit enabled the assembly of a functional proton pore [corrected]
authors.affiliation: Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra City.
authors.forename: A L
authors.initials: AL
authors.lastname: Munn
authors.forename: P R
authors.initials: PR
authors.lastname: Whitfeld
authors.forename: W
authors.initials: W
authors.lastname: Bottomley
authors.forename: G S
authors.initials: GS
authors.lastname: Hudson
authors.forename: D A
authors.initials: DA
authors.lastname: Jans
authors.forename: F
authors.initials: F
authors.lastname: Gibson
authors.forename: G B
authors.initials: GB
authors.lastname: Cox
mesh_terms: D001483:Base Sequence; D002736:Chloroplasts / Q000201:enzymology*; D003001:Cloning, Molecular; D004247:DNA; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D010944:Plants / Q000201:enzymology; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism
chemical_list: D004247:DNA; D006180:Proton-Translocating ATPases