Retinal [125I]iodomelatonin binding sites in the tree shrew (Tupaiidae).
The characteristics of melatonin-binding sites labelled by [125I]iodomelatonin in membrane preparations from the tree shrew retina were determined. Specific binding of [125I]iodomelatonin to the membrane preparations of tree shrew retina was rapid, stable, saturable, and reversible. Among the indoles tested only 6-chloromelatonin, melatonin and N-acetylserotonin had significant affinities to the [125I]iodomelatonin binding site. Scatchard analysis of the membrane preparations revealed a dissociation constant (Kd) of 51.0 +/- 16 pM and a total number of binding sites (Bmax) of 1.97 +/- 0.6 fmol/mg protein.
Lu
Y
Y
Department of Physiology, University of Hong Kong.
Yuan
H
H
Pang
S F
SF
D000818:Animals; D001667:Binding, Competitive; D007211:Indoles / Q000378:metabolism; D007700:Kinetics; D008550:Melatonin / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D044094:Receptors, Melatonin; D017981:Receptors, Neurotransmitter / Q000032:analysis* / Q000378:metabolism; D012160:Retina / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014418:Tupaiidae / Q000033:anatomy & histology* / Q000378:metabolism
D007211:Indoles; D008565:Membrane Proteins; D044094:Receptors, Melatonin; D017981:Receptors, Neurotransmitter; D008550:Melatonin