Molecular characterization and peptide specificity of two vasoactive intestinal peptide (VIP) binding sites in the chicken pineal.
Here we report that chicken pineal membranes express high affinity receptors for Vasoactive Intestinal Peptide (VIP), as revealed by competitive displacement analysis of [3-iodotyrosyl-125I]-VIP by native VIP. These binding sites were further characterized by covalent cross-linking of radioiodinated VIP to chicken pineal cell membranes, using dithiobis(succinimidylpropionate) as a cross-linking reagent. Sodium dodecyl sulfate electrophoresis after solubilization of the cross-linked membranes, reveals the existence of two polypeptides, P1 and P2, with a similar labelling intensity and apparent molecular weights of Mr = 57,000 and Mr = 70,000 respectively. These two components behave like high affinity binding sites for VIP.
Meunier
A C
AC
Laboratoire de Biologie Moléculaire (URA 1172, CNRS) Université de Poitiers, France.
Voisin
P
P
Van Camp
G
G
Cenatiempo
Y
Y
Muller
J M
JM
D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens; D003432:Cross-Linking Reagents; D000242:Cyclic AMP / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D010870:Pineal Gland / Q000737:chemistry*; D011869:Radioligand Assay; D011964:Receptors, Gastrointestinal Hormone / Q000737:chemistry*; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D003432:Cross-Linking Reagents; D007457:Iodine Radioisotopes; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP; D001191:Arylamine N-Acetyltransferase