In vitro translation and expression of renal intrinsic factor-cobalamin receptor.
The primary translation product of intrinsic factor (IF)-cobalamin receptor (IFCR) mRNA from rat kidney is a single polypeptide chain of Mr = 215,000. When expressed in Xenopus laevis oocytes the IFCR binding activity is expressed with mRNA of a size between 5 to 7 kb. These results suggest that IFCR mRNA transcripts are present in the renal tissue and encode a single chain, large molecular weight precursor. Furthermore, Xenopus oocytes can be used as a screening system in the expression cloning of the renal IFCR.
Seetharam
S
S
Department of Medicine, Medical College of Wisconsin, Milwaukee 53226.
Dahms
N
N
Li
N
N
Ramanujam
K S
KS
Seetharam
B
B
D000818:Animals; D002474:Cell-Free System / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007437:Intrinsic Factor / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008970:Molecular Weight; D009865:Oocytes / Q000378:metabolism; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011956:Receptors, Cell Surface / Q000235:genetics*; D012156:Reticulocytes / Q000378:metabolism; D014982:Xenopus laevis
D011956:Receptors, Cell Surface; C029486:intrinsic factor-cobalamin receptor; D007437:Intrinsic Factor