Cooperative binding of EF-1A to the E1A enhancer region mediates synergistic effects on E1A transcription during adenovirus infection.
A cellular nuclear factor, EF-1A, binds to a sequence motif which is repeated in the adenovirus type 5 E1A transcriptional control region. Previous genetic analyses demonstrated that two of these binding sites are predominant functional elements of the E1A enhancer region in vivo. In this report, we demonstrate that the cooperative binding of EF-1A to neighboring sites in the E1A enhancer region results in a synergistic activation of E1A transcription in infected cells.
Bruder
J T
JT
Department of Microbiology, State University of New York, Stony Brook 11794-8621.
Hearing
P
P
D000257:Adenoviridae Infections / Q000382:microbiology*; D017456:Adenovirus Early Proteins; D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000494:pharmacology; D004357:Drug Synergism; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism* / Q000494:pharmacology; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D011485:Protein Binding; D012367:RNA, Viral / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism* / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D017456:Adenovirus Early Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; D012367:RNA, Viral; D014157:Transcription Factors