title: EPR study of the hydrophobic interaction of spectrin with fatty acids.
abstract: The hydrophobic interaction between spin-labelled stearic acid and spectrin was studied by electron paramagnetic resonance (EPR) and fluorescence quenching. The results are quantitatively interpreted in terms of two types of binding site on spectrin. A comparison between the results of the EPR and fluorescence experiments show the drawback of the fluorescence method in binding studies.
authors.affiliation: Department of Hematology, Rambam Medical Center, Haifa, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Streichman
authors.forename: E
authors.initials: E
authors.lastname: Kahana
authors.forename: B L
authors.initials: BL
authors.lastname: Silver
mesh_terms: D001665:Binding Sites; D004578:Electron Spin Resonance Spectroscopy; D005453:Fluorescence; D013049:Spectrin / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism*; D014867:Water / Q000378:metabolism
chemical_list: D013229:Stearic Acids; D014867:Water; D013049:Spectrin; C031183:stearic acid