Human autoantibodies to lamin B receptor are also anti-idiotypic to certain anti-lamin B antibodies.
Autoantibodies reactive with nuclear envelope proteins are mainly detected in human sera from patients with liver diseases. Some of these antibodies are directed to lamin B, lamins A and C, or to the lamin B receptor (LBR). We show here that the latter one are anti-idiotypic to certain anti-lamin B antibodies. Using an enzyme-linked immunosorbent assay specific for lamins we found that serum M containing anti-LBR antibodies inhibited the binding to lamins of anti-lamin B autoantibodies from three of five sera tested. Similar results were obtained using patient's M purified IgG. The binding of monoclonal IgM, lambda anti-lamin B antibodies produced by a lymphoblastoid cell line derived from the patient's blood lymphocytes was also inhibited. Absorption of serum M with nuclei abolished the inhibitory activity. No inhibition was recorded with normal sera or sera containing other antinuclear specificities. Anti-LBR antibodies did not alter the binding to lamins of sera containing anti-lamins A and C antibodies. Altogether these findings demonstrate that anti-LBR antibodies are also combining site related anti-idiotypic antibodies (Ab2) to certain anti-lamin B antibodies, provide further evidence for discrete specificities among anti-lamin B antibodies and suggest that the occurrence of autoantibodies to nuclear envelope antigens may be under idiotypic regulation.
Lassoued
K
K
Laboratory of Immunochemistry and Immunopathology, Institut National de la Santé et de la Recherche Médicale U.108, Hôpital Saint-Louis, Paris, France.
Danon
F
F
Brouet
J C
JC
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis / Q000276:immunology*; D006801:Humans; D034921:Lamin Type B; D034882:Lamins; D008105:Liver Cirrhosis, Biliary / Q000276:immunology; D009685:Nuclear Envelope / Q000276:immunology; D009687:Nuclear Proteins / Q000276:immunology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000276:immunology*; D018160:Receptors, Cytoplasmic and Nuclear; D000097682:Lamin B Receptor
D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D000097682:Lamin B Receptor