[Regulation of beta-galactosidase synthesis in Escherichia coli by exogenous cyclic 3',5'-adenosine monophosphate].
The effect of cyclic 3',5'-adenosine monophosphate (cAMP) on the rate of beta-galactosidase biosynthesis was studied in the cells of Escherichia coli M-17 growing in MPB and mineral media with glucose and maltose, i.e. under the conditions of various catabolite repression, as well as upon lac-operon induction by isopropyl-beta-D-galactopyranoside (IPGP). The stimulating action of exogenous cAMP was found only in a medium with salts and glucose. The induction by IPGP was highest during the growth in a medium with glucose and maltose. When the medium contained IPGP, cAMP accelerated the enzyme synthesis in all media, but only at the early growth phases, while cAMP eliminated the effect of IPGP at the stationary phase of growth. The regulation of beta-galactosidase biosynthesis by cAMP demonstrated for the first time that this effect depended on the physiological state of E. coli: the expression of catabolite-sensitive E. coli genes was subject to both positive and negative regulation in one and the same inducible system. The effect exerted by cAMP depended on the nature of a carbon source in the growth medium.
Kaliuzhnaia
V M
VM
Korobov
V P
VP
D000242:Cyclic AMP / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D007544:Isopropyl Thiogalactoside / Q000031:analogs & derivatives / Q000494:pharmacology; D007763:Lac Operon / Q000187:drug effects / Q000502:physiology; D008320:Maltose / Q000494:pharmacology; D001616:beta-Galactosidase / Q000096:biosynthesis*
D007544:Isopropyl Thiogalactoside; C070641:isopropyl-beta-galactopyranoside; D008320:Maltose; D000242:Cyclic AMP; D001616:beta-Galactosidase; D005947:Glucose
Reguliatsiia sinteza beta-galaktozidazy Escherichia coli ékzogennym tsiklicheskim 3',5'-adenozinmonofosfatom.