title: Myocardial calcium compartmentation and contractile control.
abstract: Under the condition of rapid perfusion, the time course of contractile response of single ventricular cells to extracellular calcium (Ca) depletion and repletion identifies "fast" and "slow" cellular Ca pools. 45Ca exchange was studied in these cells under the same conditions of on-line rapid perfusion. Four kinetically-defined compartments were distinguished: (1) A "rapid" compartment containing 2.6 mmoles Ca/kg dry wt of lanthanum (La) displaceable Ca, t1/2 less than 1 sec.; (2) An "intermediate" compartment(s) containing 2.1 mmoles, t1/2 = 3 and 19 sec. Caffeine displaced significant amounts of Ca from this compartment whereas La displaced none; (3) A "slow" compartment containing 1.6 mmoles, t1/2 = 3.6 min. Addition of inorganic phosphate to the perfusate adds significant amounts of Ca to this compartment; (4) An "inexchangeable" compartment, containing 1.2 mmoles. The "rapid" compartment's flux is greater than or equal to 300 mumoles Ca/kg wet wt/sec. Its exchange rate indicates that it is the kinetic counterpart of the functionally-defined "fast" pool. Its subcellular locus is undefined. The "intermediate" compartment is best correlated with the "slow" pool and represents Ca in the sarcoplasmic reticulum. The "slow" compartment contains a significant fraction from the mitochondria. The results indicate that greater than or equal to 40% of cellular Ca can turn over within the period of one contraction cycle. These results are consistent with the following sequence: (1) Upon sarcolemmal depolarization, Ca moves through the Ca channel to arrive at the SR and at the myofilaments. (2) Ca induced Ca release occurs via the "feet" at the SR-inner SL region.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine, University of California, Los Angeles School of Medicine 90024-1760.
authors.forename: G A
authors.initials: GA
authors.lastname: Langer
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000187:drug effects* / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism; D002478:Cells, Cultured; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D012508:Sarcolemma / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D019831:Sodium-Calcium Exchanger
chemical_list: D002352:Carrier Proteins; D019831:Sodium-Calcium Exchanger; D002110:Caffeine; D000252:Calcium-Transporting ATPases; D002118:Calcium