Induction of CAT gene expression on a plasmid vector (L factor) by retinoic acid in mouse embryonal carcinoma (F9) cells.
We reported previously that composite DNA constructed from a mammalian plasmid (L factor) and foreign gene can be reestablished as a plasmid in mouse embryonal carcinoma (F9) cells after transfection and the plasmid-bearing F9 cells undergo normal in vitro differentiation in response to retinoic acid, an inducer for F9 cell differentiation. We constructed F9 cells bearing plasmidal L factor DNA in which a reporter (chloramphenicol acetyltransferase; CAT) gene was placed under the control of a differentiation-responsive viral (Moloney murine leukemia virus or simian virus 40) enhancer-promoter. When such plasmid-bearing cells were treated with retinoic acid, the CAT gene was inducibly expressed. These results indicate that mammalian gene expression can be studied with the plasmidal expression vector which is structurally dissociated from complex chromosomes.
Nishimori
K
K
Institute of Applied Microbiology, University of Tokyo, Japan.
Kohda
T
T
Segawa
K
K
Oishi
M
M
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D054278:Embryonal Carcinoma Stem Cells; D015870:Gene Expression / Q000187:drug effects; D005822:Genetic Vectors / Q000187:drug effects*; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D014411:Neoplastic Stem Cells; D010957:Plasmids / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000952:Antigens, Polyomavirus Transforming; D014212:Tretinoin; D015500:Chloramphenicol O-Acetyltransferase