Structure and expression of rat S-100 beta subunit gene.
The gene structure for S-100 beta subunit has been elucidated. The gene spans about 8 kbp and consists of 3 exons and 2 introns. The transcription initiation site was determined by an S1 nuclease mapping. The promoter region contains TATA-box-like and CAAT-box-like sequences. To examine the activity of the promoter sequence, a transfection of pS100 beta-lacZ fused gene to the cultured cells was carried out. C6 glioma cells showed a positive expression of beta-galactosidase. Gene-deletion experiments suggested the functional importance of the DNA fragment (22 bp) containing TATA-box-like and CAAT-box-like sequences. A factor protein that binds to the 100 bp DNA fragment containing the promoter sequence was specifically detected in the rat brain nuclear extract.
Maeda
T
T
Department of Neuropharmacology, Niigata University, Japan.
Usui
H
H
Araki
K
K
Kuwano
R
R
Takahashi
Y
Y
Suzuki
Y
Y
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology; D005091:Exons; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D006367:HeLa Cells / Q000502:physiology; D006801:Humans; D007438:Introns; D008099:Liver / Q000502:physiology; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D009418:S100 Proteins / Q000235:genetics*; D016385:TATA Box; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D012333:RNA, Messenger; D009418:S100 Proteins