title: Interleukin 4 inhibits murine osteoclast formation in vitro.
abstract: Interleukin 4 (IL-4) is a product of activated T cells and mast cells with effects on immunologic and hematopoietic processes. We now report that IL-4 inhibits the formation of osteoclasts from murine bone marrow cells cocultured with stromal cells. Numerous (3,000-4,000 cells/2 cm2) tartrate-resistant acid-phosphatase-positive multinucleated cells with the capacity to generate cAMP in response to salmon calcitonin (ED50 = 10(-10) M) developed within 10-12 days of culture. IL-4 (ID50 = 10 U/ml) inhibited osteoclast generation in doses similar to those that induce proliferation of IL-4-responsive T cells. Additionally, the rat antimurine IL-4 monoclonal antibody 11B11 antagonizes the IL-4-inhibitory effect on osteoclast formation. These results suggest that IL-4 impedes agonist-induced in vitro bone resorption by inhibiting osteoclastogenesis.
authors.affiliation: Department of Pathology, Jewish Hospital of St. Louis, Washington University, Missouri 63110.
authors.forename: A
authors.initials: A
authors.lastname: Shioi
authors.forename: S L
authors.initials: SL
authors.lastname: Teitelbaum
authors.forename: F P
authors.initials: FP
authors.lastname: Ross
authors.forename: H G
authors.initials: HG
authors.lastname: Welgus
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: J
authors.initials: J
authors.lastname: Ohara
authors.forename: D L
authors.initials: DL
authors.lastname: Lacey
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D016723:Bone Remodeling / Q000502:physiology; D002116:Calcitonin / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000502:physiology; D051379:Mice; D010010:Osteoclasts / Q000187:drug effects*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D015847:Interleukin-4; D002116:Calcitonin; D000242:Cyclic AMP