title: A three-step purification of manganese superoxide dismutase from human liver on both large and small scales.
abstract: A new method for the purification of manganese superoxide dismutase from human liver is described. The procedure involves essentially three steps: DEAE-cellulose, hydroxylapatite, and butyl-Toyopearl chromatographies. The method has several advantages: (i) its simplicity and rapidity (it takes less than 3 days), (ii) its high yield (62%) with a high specific activity (5660 units/mg of purified SOD), and (iii) its suitability for both large- and small-scale purifications.
authors.affiliation: Department of Biochemistry, Osaka University Medical School, Suita, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Ookawara
authors.forename: T
authors.initials: T
authors.lastname: Nakata
authors.forename: H G
authors.initials: HG
authors.lastname: Seo
authors.forename: S
authors.initials: S
authors.lastname: Kawata
authors.forename: S
authors.initials: S
authors.lastname: Tarui
authors.forename: H F
authors.initials: HF
authors.lastname: Deutsch
authors.forename: N
authors.initials: N
authors.lastname: Taniguchi
mesh_terms: D002845:Chromatography / Q000379:methods*; D002848:Chromatography, DEAE-Cellulose; D017886:Durapatite; D006801:Humans; D006882:Hydroxyapatites; D008099:Liver / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000302:isolation & purification*
chemical_list: D006882:Hydroxyapatites; D017886:Durapatite; D013482:Superoxide Dismutase