Increase in beta-1,4-galactosyltransferase activity during PC12 cell differentiation induced by forskolin and 2-chloroadenosine.
Galactosyltransferase (GALTase) activity was measured in differentiating PC12 cells induced by either forskolin or 2-chloroadenosine. The specific activity of GALTase in whole cells and isolated Golgi membranes increased as early as 3 h after initiating treatment with 2-chloroadenosine, and maximal activity was reached at approximately 12 h. In two mutant PC12 cell lines deficient in protein kinase A, both forskolin and 2-chloroadenosine failed to increase GALTase activity. The adenosine A2 receptor antagonist, xanthine amine congener, prevented 2-chloroadenosine stimulation of GALTase, demonstrating that this adenosine derivative was mediating its effect via the A2 receptor. These data suggest that GALTase activity during PC12 cell differentiation is regulated by cyclic AMP (cAMP)- and protein kinase A-dependent processes. In support of the role of cAMP in regulating GALTase activity were studies with murine PC carcinoma cells demonstrating that the greatest stimulation of GALTase activity occurred with cells treated with both retinoic acid and dibutyryl cAMP.
Roth
J A
JA
Department of Pharmacology, State University of New York, Buffalo 14214.
Marcucci
K
K
Lin
W H
WH
Napoli
J L
JL
Wagner
J A
JA
Rabin
R
R
D015762:2-Chloroadenosine / Q000494:pharmacology*; D000310:Adrenal Gland Neoplasms; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology; D007700:Kinetics; D010673:Pheochromocytoma; D051381:Rats; D014212:Tretinoin / Q000494:pharmacology; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase / Q000378:metabolism*
D015762:2-Chloroadenosine; D005576:Colforsin; D014212:Tretinoin; D000242:Cyclic AMP; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase