title: [Temperature-sensitive clones of herpes simplex virus type 1 from laboratory and clinical strains. I. Cloning and basic pathogenetic and immunogenic properties].
abstract: Two HSV-1 strains were used in the study: McIntyre laboratory strain and "eye" strain isolated from a patient. Temperature-sensitive clone of HSV-1 was isolated from McIntyre strain as a consequence of virus replication carried out at lowered temperature (28 degrees C). Temperature-resistant clones were obtained from both strains through passages at 39 degrees C and through heating for four times at 45 degrees C. Pathogenic properties of the temperature clones obtained were determined in inbred mice Balb/c and CFw/Pzh. A loss of pathogenicity for mice of temperature-sensitive clone and an increase of pathogenicity of temperature-resistant clones were noted as compared to parental strains. It was found that an introduction of temperature-sensitive clone, with lowered virulence immunizes against highly virulent temperature-resistant clone.
authors.affiliation: Zakład Wirusologii PZH w Warszawie.
authors.forename: B
authors.initials: B
authors.lastname: Litwińska
authors.forename: W
authors.initials: W
authors.lastname: Sadowski
authors.forename: M
authors.initials: M
authors.lastname: Kańtoch
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006561:Herpes Simplex / Q000209:etiology / Q000382:microbiology*; D006801:Humans; D007114:Immunization; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D018139:Simplexvirus / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D013696:Temperature; D014774:Virulence / Q000276:immunology / Q000502:physiology; D014779:Virus Replication / Q000276:immunology / Q000502:physiology*
vernacular_title: Klony temperaturowe wirusa herpes simplex typu 1 wywodzace sieze szczepów laboratoryjnego i klinicznego. I. Klonowanie oraz podstawowe właściwości patogenne i immunogenne.