title: A dominant mutation in the gene for the Nag repressor of Escherichia coli that renders the nag regulon uninducible.
abstract: The gene nagC encodes the repressor for the nag regulon. A point mutation within the gene, which confers a super-repressor phenotype and makes the repressor insensitive to the inducer, N-acetylglucosamine 6-phosphate, has been characterized. The mutation is semi-dominant since heterozygous diploids have reduced growth rates on glucosamine and N-acetylglucosamine compared to the wild-type strain.
authors.affiliation: Institut de Biologie Physico-chimique (URA1139), Paris, France.
authors.forename: J A
authors.initials: JA
authors.lastname: Plumbridge
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000378:metabolism; D000581:Amidohydrolases / Q000096:biosynthesis* / Q000235:genetics; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004171:Diploidy; D004790:Enzyme Induction / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005799:Genes, Dominant / Q000235:genetics; D005944:Glucosamine / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C067862:nagC protein, E coli; C010004:N-acetylglucosamine 6-phosphate; D001616:beta-Galactosidase; D000581:Amidohydrolases; C018717:N-acetylglucosamine-6-phosphate deacetylase; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase; D005944:Glucosamine; D000117:Acetylglucosamine