title: Isolation in Italy of feline immunodeficiency virus (FIV).
abstract: The Authors report the isolation in Italy of Feline Immunodeficiency Virus (FIV) from a cat inoculated with whole blood from a naturally FIV infected cat. The virus was isolated in feline circulating leucocytes cultured in RPMI medium and stimulated with concanavalin-A and recombinant human interleukin-2. The infected cultures showed a characteristic cytopathic effect (ballooning degeneration, giant cell formation, cell death) and a specific fuorescence using FIV-positive cat serum and monoclonal antibodies against FIV. Furthermore, the culture supernatants contained magnesium-dependent reverse transcriptase activity.
authors.affiliation: Istituto di Patologia delle Malattie Infettive e Parassitarie degli animali domestici, Facoltà di Medicina Veterinaria, Bari.
authors.forename: C
authors.initials: C
authors.lastname: Buonavoglia
authors.forename: M
authors.initials: M
authors.lastname: Tempesta
authors.forename: M G
authors.initials: MG
authors.lastname: Pennisi
authors.forename: L
authors.initials: L
authors.lastname: Di Trani
authors.forename: F
authors.initials: F
authors.lastname: Marsilio
authors.forename: F
authors.initials: F
authors.lastname: Titti
authors.forename: O
authors.initials: O
authors.lastname: Catarsini
authors.forename: M
authors.initials: M
authors.lastname: Compagnucci
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002415:Cats; D002470:Cell Survival; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D016181:Feline Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D016089:Immunodeficiency Virus, Feline / Q000276:immunology / Q000302:isolation & purification*; D007376:Interleukin-2 / Q000494:pharmacology; D007558:Italy; D007962:Leukocytes / Q000382:microbiology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D011994:Recombinant Proteins; D003208:Concanavalin A