title: [Determination of methadone in plasma using high pressure liquid chromatography methods].
abstract: High-performance liquid chromatography on the column of LiChrosorb RP-18 was applied to the determination of methadone in plasma, after previous precipitation of proteins with methanol and extraction from alkalized plasma with diethyl ether. Methadone, ion-paired with pentane-1-sulphonic acid added in a mobile phase acetonitrile-methanol-ammonium acetate solution (20:58:22), was monitored at 254 nm. Piritramide was used as an internal standard. The determination range was 0.25-1.25 micrograms/cm3 of plasma and recovery was 88-93%.
authors.affiliation: Katedra Chemii Leków, Akademia Medyczna, Lublin.
authors.forename: G
authors.initials: G
authors.lastname: Misztal
authors.forename: L
authors.initials: L
authors.lastname: Przyborowski
authors.forename: A
authors.initials: A
authors.lastname: Wojtczak
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008691:Methadone / Q000097:blood*
chemical_list: D008691:Methadone
vernacular_title: Oznaczanie metadonu w osoczu metoda wysokosprawnej chromatografii cieczowej.