Model for studying virus attachment. II. Binding of biotinylated human T cell leukemia virus type I to human blood mononuclear cells potential targets for human T cell leukemia virus type I infection.
Purified human T cell leukemia virus type I (HTLV-I) was biotinylated and used to study its attachment to human PBMC. The use of biotinylated HTLV-I (biot-HTLV-I) in conjunction with mouse mAb specific for selected cell-surface molecules and flow cytometric analysis allowed us to positively identify virus-binding cells among a heterogeneous blood mononuclear cell population. Biot-HTLV-I efficiently bound not only to T cells, but also to B cells and monocytes. Preincubation of monocytes with excess of unlabeled HTLV-I significantly reduced the attachment of biot-HTLV-I. HTLV-I not only bound to, but also infected, B cells, as suggested by: i) in situ hybridization of a 35S-labeled full length HTLV-I DNA probe with EBV-transformed B cells, previously cocultured with HTLV-I-producing (G11MJ) T cells, and ii) hybridization of the same nick-translated 32P-labeled DNA probe with blotted DNA from similar HTLV-I-infected EBV-transformed B cells. HTLV-I infection did not affect the ability of B cells to secrete IgG. These findings suggest that HTLV-I cannot only infect cells of the T lineage, but can also infect B cells.
Dhawan
S
S
Laboratory of Oral Medicine, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
Streicher
H Z
HZ
Wahl
L M
LM
Miller
N
N
Louie
A T
AT
Goldfarb
I S
IS
Jackson
W L
WL
Casali
P
P
Notkins
A L
AL
D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism / Q000382:microbiology; D001667:Binding, Competitive; D001710:Biotin; D002472:Cell Transformation, Viral; D005434:Flow Cytometry; D015490:HTLV-I Infections / Q000382:microbiology*; D004854:Herpesvirus 4, Human; D015368:Human T-lymphotropic virus 1 / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000382:microbiology*; D009000:Monocytes / Q000378:metabolism / Q000382:microbiology; D011991:Receptors, Virus / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism / Q000382:microbiology
D011991:Receptors, Virus; D001710:Biotin