[5-Variable genome sequence of the LIVP vaccinia virus. A possible role for short direct repeats in the formation of deletions].
HindIII-O/N DNA fragments of vaccinia virus (VV) of the LIVP strain were mapped using thirteen restriction endonucleases. Nucleotide sequences of the HindIII-O fragment (1530 bp) as well as of a site of the HindIII-N genome fragment 353 bp in size were determined. Comparison of restriction maps and nucleotide sequences of VV strains (WR and LIVP) demonstrated that DNA of VV LIVP contained % deletions and 2 insertions. "Reliable" short direct repeats were localized and their possible role in formation of DNA deletions was shown. It was suggested that VV endonuclease and DNA-ligase participate in replication and repair processes. Mechanism of formation of variable sequences of viral genomes is discussed.
Prikhod'ko
G G
GG
Babkin
I V
IV
D000595:Amino Acid Sequence; D001483:Base Sequence; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014616:Vaccinia virus / Q000235:genetics*
D004262:DNA Restriction Enzymes
5-Variabel'naia posledovatel'nost' genoma virusa ospovaktsiny shtamma LIVP. Vozmozhnaia rol' korotkikh priamykh povtorov v formirovanii deletsiĭ.