Comparison of two methods for rapid detection of cytomegalovirus viraemia in cell culture.
Two methods for rapid CMV viraemia detection in cell culture with immunoperoxidase staining performed 40 h post-inoculation were compared, on 230 blood samples: (1) a 24-well plate centrifugation assay and (2) a 25-cm2-culture flask technique, without centrifugation. The flask technique was significantly more sensitive than the 24-well plate technique. Moreover, the flask assay was more cost-effective and offered other advantages such as easier sterile handling and less damage of the cell monolayer.
Pépin
J M
JM
Department of Virology, Hôpital Claude Bernard, Paris.
Simon
M
M
Simon
F
F
Dazza
M C
MC
Brun-Vézinet
F
F
D002478:Cells, Cultured; D002498:Centrifugation; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000150:complications / Q000382:microbiology; D005347:Fibroblasts / Q000382:microbiology; D015658:HIV Infections / Q000150:complications / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity