Evidence for the generation of hydroxyl radicals from a chromium(V) intermediate isolated from the reaction of chromate with glutathione.
The formation of hydroxyl radicals from a chromium(V) complex isolated from the reaction of glutathione with chromate has been demonstrated in spin trapping experiments using dimethylsulfoxide and 3,5-dibromo-4-nitrosobenzene sulfonate. Mechanisms for the formation of radicals in such systems are discussed. These results help to explain the ability of solutions containing chromate and glutathione to cause strand breaks in DNA.
Jones
P
P
Department of Chemistry, Queen Mary and Westfield College, University of London, United Kingdom.
Kortenkamp
A
A
O'Brien
P
P
Wang
G
G
Yang
G
G
D002840:Chromates; D002857:Chromium; D004121:Dimethyl Sulfoxide; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D005609:Free Radicals; D005978:Glutathione; D006878:Hydroxides; D017665:Hydroxyl Radical; D007700:Kinetics
D002840:Chromates; D016166:Free Radical Scavengers; D005609:Free Radicals; D006878:Hydroxides; D002857:Chromium; D017665:Hydroxyl Radical; D005978:Glutathione; D004121:Dimethyl Sulfoxide