Presence of 5-methylcytosine in CC(A/T)GG sequences (Dcm methylation) in DNAs from different bacteria.
The presence of CC(A/T)GG sequences with methylated internal cytosine (Dcm methylation) was determined in DNA from different genera of eubacteria. This methylation was studied by using restriction enzymes EcoRII and BstNI, which cleave unmethylated or methylated CC(A/T)GG sequences. Dcm methylation was only detected in genera of the family Enterobacteriaceae closely related to Escherichia: Shigella, Citrobacter, Salmonella, and Klebsiella.
Gomez-Eichelmann
M C
MC
Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City.
Levy-Mustri
A
A
Ramirez-Santos
J
J
D044503:5-Methylcytosine; D001419:Bacteria / Q000737:chemistry*; D001483:Base Sequence; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification
D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D044503:5-Methylcytosine; D003596:Cytosine; D004262:DNA Restriction Enzymes