Mutagenicity of 2-amino-N6-hydroxyadenine (AHA) at three loci in L5178Y/tk+/- mouse lymphoma cells: molecular and preliminary cytogenetic characterizations of AHA-induced tk-/- mutants.
2-Amino-N6-hydroxyadenine (AHA) is a remarkably efficient and specific inducer of point mutations in Neurospora, with few or no larger scale events being detected (de Serres et al., 1985). In the present studies, AHA is shown to be a potent point mutagen at the tk +/-, hprt+ and Na+/K+ ATPase loci in L5178Y/tk (+/-)-3.7.2C mouse lymphoma cells. Both large and small colony tk-/- mutants were analyzed at the molecular level and a preliminary assessment was made of small colony mutant karyotypes (230 bands/haploid metaphase cell; large colony mutants typically have normal karyotypes and were not analyzed). AHA induced greatly delayed (7-9 cell doublings) cytotoxicity, suggestive of a mutational mechanism (e.g., base-pair substitution) requiring DNA replication prior to its phenotypic expression. Approximately one-third of the tk -/- mutants formed small colonies, a phenotype which is typically associated with alterations to chromosome 11b, the site of the functional tkb allele in the parental cells. However, banded karyotypes have provided convincing evidence for alterations chromosome 11b in only 2 of the 7 small colony mutants analyzed. Southern blot analysis showed that 78% (21/27) of these small colony mutants have retained the Nco-1 6.3-kb band, which is diagnostic of the tkb allele. This makes AHA unique among the mutagens examined so far in inducing small colony mutants without inducing large losses of tkb DNA. Although a dose-dependent increase in the proportion of small colony mutants was noted, no significant dose-dependent differences were seen at the molecular level in the relatively few mutants analyzed. The majority of AHA-induced tk -/- mutants formed large colonies. Southern blot analysis showed that 86% (25/29) of these had retained the Nco-1 6.3-kb band which is diagnostic of the tkb allele. It is concluded that AHA induces primarily micromutations (less than 100 base pairs), probably through a base-pair substitution mechanism, at the tk, hprt and Na+/K+ ATPase loci in this system, with some larger scale damage (kilobases of DNA at the molecular level; chromosome 11b damage at the cytogenetic level) also occurring.
Clive
D
D
Wellcome Research Laboratories, Research Triangle Park, NC 27709.
Glover
P
P
Krehl
R
R
Poorman-Allen
P
P
D000225:Adenine / Q000031:analogs & derivatives* / Q000633:toxicity; D000818:Animals; D015139:Blotting, Southern; D002470:Cell Survival / Q000187:drug effects; D002871:Chromosome Banding; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D066298:In Vitro Techniques; D008223:Lymphoma; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014407:Tumor Cells, Cultured
D009153:Mutagens; C030632:2-amino-N(6)-hydroxyadenine; D007041:Hypoxanthine Phosphoribosyltransferase; D013937:Thymidine Kinase; D000254:Sodium-Potassium-Exchanging ATPase; D000225:Adenine