Iron is the intracellular metal involved in the production of DNA damage by oxygen radicals.
The metal chelators 1,10-phenanthroline and 2,9-dimethyl-1,10-phenanthroline (neocuproine) showed distinct abilities to prevent hydroxyl radical formation from hydrogen peroxide and Cu+ or F2(2+) (Fenton reaction) as determined by electron spin resonance. o-Phenanthroline prevented both Fe- and Cu-mediated Fenton reactions whereas neocuproine only prevented the Cu-mediated Fenton reaction. Because only 1,10-phenanthroline but not neocuproine prevented DNA strand-break formation in hydrogen peroxide-treated mammalian fibroblasts it appears that the Fe-mediated, as compared to the Cu-mediated, intranuclear Fenton reaction is responsible for DNA damage.
Mello-Filho
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AC
Department of Biochemistry, University of São Paulo, Brazil.
Meneghini
R
R
D016475:3T3 Cells; D000818:Animals; D002461:Cell Line, Transformed; D004249:DNA Damage; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D007501:Iron / Q000378:metabolism*; D051379:Mice; D010100:Oxygen / Q000378:metabolism*; D010618:Phenanthrolines / Q000494:pharmacology
D005609:Free Radicals; D010618:Phenanthrolines; C002701:neocuproine; D007501:Iron; D010100:Oxygen; C025205:1,10-phenanthroline