Pentamer arrangements in fragments of human rotavirus inner capsids observed by low dose electron microscopy.
Fragments of disrupted capsids were frequently seen in purified preparations of human rotavirus inner capsids by negative staining method in electron microscopy. The ultrastructures of these fragments were analyzed in comparison with the T = 13L model. Well-resolved pentamers were rarely seen in the fragments on observation at the standard electron doses, but were frequently seen at low electron doses, which meant a better preservation of the steric structure of capsid fragments in the latter case. Thus advantages of low dose electron microscopy have been shown in observation of capsid fragments.
Hosaka
Y
Y
Department of Microbiology, Osaka University of Pharmaceutical Sciences, Japan.
Akahori
H
H
Hernandez
F
F
Akaike
K
K
Ikegami
N
N
D002213:Capsid / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods; D012401:Rotavirus / Q000648:ultrastructure*