Killer cells induced by stimulation with allogeneic tumor cells and subsequent culture with recombinant interleukin-2.
Peripheral blood lymphocytes were cultured for 5 days with allogeneic tumor cells (allogeneic mixed lymphocyte/tumor cell culture), and subsequently cultured with recombinant interleukin-2 for 12 days. These cultured cells were found to be cytotoxic to autologous tumor cells. Results of two-color analysis using monoclonal antibodies to cell markers showed that more than 80% of their cultured cells were CD3+ cells, and CD4+ cells showed a higher distribution than CD8+ cells. However, CD8+ cells had a much higher killing activity with autologous tumor than did CD4+ cells, when estimated by an elimination study using monoclonal antibodies to T cell phenotypes and complement. The "cold-target" inhibition test showed that the cytotoxicity of these cells for autologous tumor cells was inhibited by unlabeled autologous tumor cells but not by unlabeled stimulator cells. Furthermore, about 40% of the cytotoxicity was suppressed by blocking of HLA class I antigen with a monoclonal antibody on autologous tumor cells. Thus, cytotoxic activity of lymphocytes to autologous tumor restricted by target cell HLA class I antigen is possibly induced by allogeneic tumor-stimulation.
Fukiage
T
T
Department of Otolaryngology, Kumamoto University, School of Medicine, Japan.
Murakami
H
H
Eura
M
M
Ikawa
T
T
Ishikawa
T
T
D000231:Adenocarcinoma, Papillary / Q000276:immunology; D000328:Adult; D000368:Aged; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis; D003528:Carcinoma, Adenoid Cystic / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D005260:Female; D015788:HLA-A1 Antigen; D006801:Humans; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000494:pharmacology*; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D015788:HLA-A1 Antigen; D007376:Interleukin-2