Laboratory studies of a Brazilian strain of Aedes albopictus as a potential vector of Mayaro and Oropouche viruses.
The vector efficiency of colonized Aedes albopictus from Brazil was assessed for Mayaro (MAY) and Oropouche (ORO) viruses. Female mosquitoes, 3-4 days old, were fed on a MAY-infected hamster with a viremia level of 5.3 log10 Vero cell plaque-forming units (PFU) of virus/ml or an ORO-infected hamster circulating 7.3 log10 PFU/ml. Mayaro infection rates among fed mosquitoes were 16.9 and 11% at 6, 13 and 20 days postfeeding, respectively, and 1/2 and 2/2 infected mosquitoes transmitted virus on days 13 and 20, respectively. Only 13, 5 and 3% of mosquitoes were infected with ORO virus at 6, 13 and 20 days, respectively, and no transmission occurred. Mosquitoes were also fed on 3 dilutions of MAY virus-blood suspensions in membrane feeders. The infection rate among mosquitoes fed the highest concentration (7.7 log10 PFU/ml) was 11/13 (85%), and 5/11 (46%) infected mosquitoes transmitted virus.
Smith
G C
GC
Division of Vector-Borne Infectious Diseases, Centers for Disease Control, Fort Collins, CO 80522.
Francy
D B
DB
D000330:Aedes / Q000382:microbiology; D000524:Alphavirus / Q000302:isolation & purification; D000818:Animals; D002043:Bunyaviridae / Q000302:isolation & purification; D002044:Bunyaviridae Infections / Q000635:transmission*; D006224:Cricetinae; D005260:Female; D007303:Insect Vectors / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology; D051379:Mice; D012469:Salivary Glands / Q000382:microbiology; D014036:Togaviridae Infections / Q000635:transmission*