title: Ubiquinone protects against loss of tocopherol in rat liver microsomes and mitochondrial membranes.
abstract: Liver microsomes and submitochondrial particles (SMP) were isolated from rats fed on a vitamin E or coenzyme Q10/vitamin E enriched diet in order to clarify the antioxidant interactions between coenzyme Q10 and vitamin E. Electron spin resonance spectrometry shows that the decay of vitamin E radicals (tocopheroxy radicals) generated by the arachidonic acid/lipoxygenase oxidation system proceeded at a higher rate in vitamin E enriched microsomes and SMP than in those enriched with coenzyme Q10/vitamin E. Vitamin E levels determined by high performance liquid chromatography revealed that when subjected to enzymatic oxidation, membranes enriched with vitamin E alone were depleted of vitamin E earlier than those enriched with both coenzyme Q10 and vitamin E. These results show that coenzyme Q10 conserves vitamin E, which would help prolong membrane resistance against oxidative damage.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley 94720.
authors.forename: M
authors.initials: M
authors.lastname: Hiramatsu
authors.forename: R D
authors.initials: RD
authors.lastname: Velasco
authors.forename: D S
authors.initials: DS
authors.lastname: Wilson
authors.forename: L
authors.initials: L
authors.lastname: Packer
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013367:Submitochondrial Particles / Q000187:drug effects / Q000378:metabolism; D014451:Ubiquinone / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D014810:Vitamin E / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D014451:Ubiquinone; D014810:Vitamin E