Interleukin-1 alpha modulates luteinizing hormone stimulated cyclic AMP and progesterone release from human granulosa cells in vitro.
This study examines the possible direct effect of interleukin-1 alpha (IL-1 alpha) upon human granulosa cells. The cells were isolated from follicles of stimulated cycles in women undergoing oocyte retrieval for in-vitro fertilization. Purified cell preparations were cultured for different time periods in the presence of IL-1 alpha and human luteinizing hormone (LH) or follicle stimulating hormone. IL-1 alpha stimulated basal as well as LH-induced progesterone accumulation. The response in terms of cyclic AMP was more complex, there was no effect of IL-1 alpha on basal cyclic AMP accumulation. However, at the highest concentration tested (50 IU/ml), IL-1 alpha enhanced cyclic AMP accumulation over that seen with LH alone. At a lower concentration, IL-1 alpha either had no effect or was slightly inhibitory to the LH-induced cyclic AMP accumulation, depending on the culture period. Our results, taken together with other findings, are compatible with the view that IL-1 alpha has a potential regulatory role in the granulosa-luteal cell transition in the human ovary.
Sjögren
A
A
Department of Obstetrics and Gynaecology, University of Göteborg, Sahlgrenska Hospital, Sweden.
Holmes
P V
PV
Hillensjö
T
T
D002478:Cells, Cultured; D003338:Corpus Luteum / Q000166:cytology / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000502:physiology; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology
D007375:Interleukin-1; D011994:Recombinant Proteins; D011374:Progesterone; D007986:Luteinizing Hormone; D000242:Cyclic AMP