title: An assessment of the role of proton leaks in the mechanistic stoichiometry of oxidative phosphorylation.
abstract: Rat liver mitochondria were incubated in the presence of varying concentrations of ATP, followed by ADP to initiate phosphorylation. Analysis of phosphorylation to oxygen ratios (P/O) was carried out with varied initial phosphorylation potentials (or ATP/ADP ratios). Rates of phosphorylation and respiration and magnitude of membrane potential (delta psi) were measured. The results are discussed in the framework of P/total O and P/"extra" O ratios in determination of the mechanistic P/O ratio. It is concluded that the former underestimates, and the latter overestimates the mechanistic P/O ratio.
authors.affiliation: Indiana University School of Medicine, Department of Biochemistry and Molecular Biology, Indianapolis 46202-5122.
authors.forename: E J
authors.initials: EJ
authors.lastname: Davis
authors.forename: W I
authors.initials: WI
authors.lastname: Davis-van Thienen
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010766:Phosphorylation; D011522:Protons; D051381:Rats
chemical_list: D011522:Protons; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate