Inflammatory cell influx into ozone-exposed guinea pig lung interstitial and airways spaces.
As a model of non-immunological lung inflammation, guinea pigs were exposed to 2.0 ppm ozone for 4 hours. Polymorphonuclear leukocytes (PMNs) rapidly accumulated in lung interstitium but declined from 147 +/- 34 million cells to control values (33 +/- 6 million cells) within the first 24 hours. Bronchoalveolar lavage (BAL) recovered PMNs were maximal by 3-6 hours (4 +/- 1 million cells) and remained elevated for 3 days. Macrophage numbers were doubled in lavageable spaces but those in interstitium increased by only a third at 2 days post-exposure. By 7 days post-exposure BAL macrophages had declined to control values of 12 +/- 2 million cells while those in interstitium remained elevated through 14 days. These data demonstrate that BAL does not necessarily reflect cellular changes in lung interstitium.
Schultheis
A H
AH
Environmental Health Sciences Center, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205.
Bassett
D J
DJ
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D005110:Extracellular Space / Q000378:metabolism; D006168:Guinea Pigs; D007249:Inflammation / Q000473:pathology; D008168:Lung / Q000201:enzymology; D008171:Lung Diseases / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D003012:Microbial Collagenase / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology; D010126:Ozone
D010126:Ozone; D003012:Microbial Collagenase