Evaluation of 3H-CGP 12177 and 3H-DHA binding to beta 2-adrenoceptors of rat reticulocytes by means of affinity spectra.
3H-CGP 12177 and 3H-DHA binding to rat reticulocyte beta 2-adrenoceptors by using an excess of 1 x 10(-5) mol l-1 propranolol was evaluated by means of affinity spectra. To describe the 3H-CGP 12177 interaction with rat reticulocytes a binding isotherm valid for one single class of specific binding sites was found to be satisfactory. On the other hand, a more complex binding isotherm had to be used for the description of the 3H-DHA interaction with rat reticulocytes. This observation further supports the preferred application of 3H-CGP 12177 over 3H-DHA for radio-receptor assay of beta-adrenoceptor blockers.
Kállay
Z
Z
Institute of Preventive and Clinical Medicine, Bratislava, Czecho-Slovakia.
Smisterová
J
J
Soltés
L
L
Marko
V
V
D000818:Animals; D001665:Binding Sites; D004082:Dihydroalprenolol / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D011412:Propanolamines / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012156:Reticulocytes / Q000378:metabolism*
D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; C033394:CGP 12177