title: Enhanced lipid peroxidation in lung lavage of rats after inhalation of asbestos.
abstract: Exposure of phagocytic cells to asbestos in vitro results in an augmented production of reactive oxygen metabolites and increased peroxidation of lipids. The aim of this investigation was to assess the extent of lipid peroxidation both in cells and fluid obtained from bronchoalveolar lavage (BAL), and in lungs of rats exposed to crocidolite asbestos or titanium dioxide (TiO2), a nonfibrous particulate control. In comparison to sham and TiO2-exposed rats, the BAL fluid and cells of crocidolite-exposed animals contained significantly elevated levels of malondialdehyde (MDA), a breakdown product of lipid peroxidation detected using high-pressure liquid chromatography (HPLC). In contrast, no significant differences in MDA were detected in lavaged lung tissue from these animals. Inhalation of crocidolite caused an early inflammatory response characterized by elevated numbers of polymorphonuclear leukocytes and lymphocytes, as well as enhanced total protein in BAL. Pulmonary fibrosis and increased lung hydroxyproline also were observed after 20 days of exposure. Exposure to TiO2 did not cause inflammation, pulmonary fibrosis, or elevated amounts of hydroxyproline in the lung. Our results show that exposure to the fibrogenic and inflammatory mineral, crocidolite, results in an enhanced lipid peroxidation in BAL cells and fluid not observed after inhalation of the particulate TiO2. These novel observations suggest that MDA in BAL may be useful as a biomarker of exposure to inhaled asbestos or other oxidants.
authors.affiliation: Department of Pathology, University of Vermont College of Medicine, Burlington 05405-0068.
authors.forename: J M
authors.initials: JM
authors.lastname: Petruska
authors.forename: K O
authors.initials: KO
authors.lastname: Leslie
authors.forename: B T
authors.initials: BT
authors.lastname: Mossman
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001194:Asbestos / Q000008:administration & dosage / Q000494:pharmacology*; D017638:Asbestos, Crocidolite; D001992:Bronchoalveolar Lavage Fluid / Q000378:metabolism*; D003094:Collagen / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects*; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D014025:Titanium / Q000494:pharmacology
chemical_list: D017638:Asbestos, Crocidolite; D001194:Asbestos; C009495:titanium dioxide; D008315:Malondialdehyde; D003094:Collagen; D014025:Titanium; D006909:Hydroxyproline