[3H]nitrendipine binding in temporal cortex in Alzheimer's and Huntington's diseases.
Specific [3H]nitrendipine binding which was shown to be calcium- and calmodulin-dependent was found to be significantly reduced in the temporal cortex in Alzheimer's disease compared to age-matched controls. Scatchard analysis revealed that this reduction was due to a loss in the number of cortical [3H]nitrendipine binding sites rather than a change in the affinity of the binding site in the Alzheimer patients. The reduction in cortical [3H]nitrendipine-specific binding was most marked in those Alzheimer's disease cases where the duration of the dementing illness was longer than two years. In contrast, no reduction in cortical [3H]nitrendipine binding was found in Huntington's disease. There was no significant correlation found between age (38-89 years) and [3H]nitrendipine binding in control cases, or between mean overall plaque counts and [3H]nitrendipine binding in the Alzheimer's disease cases. There was a significant correlation found between age (46-88 years) and [3H]nitrendipine binding in the Alzheimer's disease cases where the duration of the dementing illness was greater than two years.
Piggott
M A
MA
MRC Neurochemical Pathology Unit, Newcastle General Hospital, Newcastle upon Tyne U.K.
Candy
J M
JM
Perry
R H
RH
D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D001667:Binding, Competitive; D002118:Calcium / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels; D006801:Humans; D006816:Huntington Disease / Q000378:metabolism* / Q000473:pathology; D007093:Imidazoles / Q000494:pharmacology; D007700:Kinetics; D008875:Middle Aged; D009568:Nitrendipine / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000378:metabolism*; D012016:Reference Values; D013702:Temporal Lobe / Q000378:metabolism* / Q000473:pathology; D014316:Tritium
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D007093:Imidazoles; D011978:Receptors, Nicotinic; D014316:Tritium; C031938:calmidazolium; D009568:Nitrendipine; D002118:Calcium