title: Action potential waveform voltage-clamp commands reveal striking differences in calcium entry via low and high voltage-activated calcium channels.
abstract: Calcium channels transduce natural voltage transients, like action potentials, into functionally important intracellular calcium transients. We have used digitally constructed waveforms that simulate natural action potentials as voltage-clamp commands to study channel function in transduction. Whole-cell calcium currents elicited by several action potential waveforms (APWs) were studied. The currents were subdivided into T (or low voltage-activated) and high voltage-activated components. Calcium entry through T channels constituted a disproportionately large fraction of the total during normal, brief APWs. Entry through high voltage-activated channels was much more responsive to APW, increasing more significantly as APW duration increased. Thus the results indicate that differences in the gating properties of these two channel classes combine to endow them with strikingly different transducer properties.
authors.affiliation: Department of Physiology, Colorado State University, Fort Collins 80523.
authors.forename: D P
authors.initials: DP
authors.lastname: McCobb
authors.forename: K G
authors.initials: KG
authors.lastname: Beam
mesh_terms: D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism / Q000502:physiology*; D002642:Chick Embryo; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D007700:Kinetics; D009474:Neurons / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D002118:Calcium