Platelet 3H-imipramine binding sites in obsessive-compulsive behavior.
Several studies indicate a serotonergic dysfunction in patients with obsessive-compulsive disorder (OCD). We examined serotonergic function in OCD by determining platelet 3H-impiramine binding sites in patients with OCD during a drug-free baseline period as well as normal control volunteers. The maximum number of binding sites (Bmax) and apparent dissociation constant (Kd) was determined using 3H-imipramine (IMI) as the binding ligand. We observed that the mean 3H-IMI binding Bmax (fmol/mg protein) determined in 24 patients with OCD was not significantly different from that in 23 normal control subjects. There were no significant differences in the Kd between patients with OCD and normal control subjects. Our results are thus similar to those reported by Insel et al (1985) and Black et al (1990), who observed no significant differences in platelet 3H-IMI binding between OCD patients and controls; but different from those reported by Weizmann et al (1986), who observed decreased 3H-IMI Bmax in OCD patients. The discrepancy in the results is not clear, but may be related to several factors. Our results thus indicate that any abnormality in serotonergic function present in patients with OCD is not related to imipramine binding sites in the platelets. However, the possibility that there may be an abnormal platelet serotonin uptake or other serotonergic function in OCD cannot be ruled out.
Kim
S W
SW
Department of Psychiatry, Hennepin County Medical Center, Minneapolis, MN 55414.
Dysken
M W
MW
Pandey
G N
GN
Davis
J M
JM
D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D002352:Carrier Proteins; D002997:Clomipramine / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007099:Imipramine / Q000493:pharmacokinetics*; D008297:Male; D008875:Middle Aged; D009771:Obsessive-Compulsive Disorder / Q000097:blood* / Q000188:drug therapy / Q000523:psychology; D011569:Psychiatric Status Rating Scales; D011869:Radioligand Assay; D011955:Receptors, Drug; D017981:Receptors, Neurotransmitter / Q000502:physiology*
D002352:Carrier Proteins; D011955:Receptors, Drug; D017981:Receptors, Neurotransmitter; C027775:imipramine receptor; D002997:Clomipramine; D007099:Imipramine