[The use of lanthanide immunofluorescence analysis for demonstrating the antigen of viral hepatitis A].
The study dealt with the development of an express method for detection of hepatitis A virus (HAV) antigen employing lantanides, especially europium. The new time-resolved fluoroimmunoassay (TRFIA), alongside with a significant shortening of the time for reaction, was also 8 times as sensitive as the analogous enzyme immunoassay. The TRFIA may be used effectively both for control of the antigen amplification in HAV-infected cell culture and for early diagnosis of acute forms of viral hepatitis A.
Chernovetskiĭ
M A
MA
Sokolova
M V
MV
Kharitonenkov
I G
IG
D000956:Antigens, Viral / Q000097:blood*; D005069:Evaluation Studies as Topic; D015200:Fluoroimmunoassay / Q000379:methods*; D006507:Hepatovirus / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008674:Metals, Rare Earth
D000956:Antigens, Viral; D008674:Metals, Rare Earth
Primenenie lantanidnogo immunofliuorestsentnogo analiza dlia indikatsii antigena virusnogo gepatita A.