title: Superoxide dismutases in Eimeria tenella.
abstract: Unsporulated oocysts of Eimeria tenella have high superoxide dismutase (SOD: superoxide:superoxide oxidoreductase, EC 1.15.1.1.) activity and contain several electrophoretically distinct forms of the enzyme, including two forms of Cu/Zn-containing SOD, two forms of Fe-SOD and two forms of Mn-SOD. SOD activity remains high during 12 h of sporulation but diminishes slowly during prolonged sporulation. Oocysts sporulated for 48 h have low levels of superoxide dismutase and contain only one form of the enzyme (Mn-SOD), which was also found in sporozoites. In vitro, sporozoites are oxidant-sensitive and die within minutes of superoxide radical (O2-) generation but SOD/catalase and mannitol protect sporozoites against oxidative damage. These data suggest that E. tenella sporulated oocysts and sporozoites lack soluble cytoplasmic SOD and that this deficiency may contribute to the oxidant sensitivity of the parasite.
authors.affiliation: CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.
authors.forename: W P
authors.initials: WP
authors.lastname: Michalski
authors.forename: S J
authors.initials: SJ
authors.lastname: Prowse
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism / Q000494:pharmacology; D004539:Eimeria / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D005260:Female; D007527:Isoenzymes / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D013170:Spores / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism* / Q000494:pharmacology; D013481:Superoxides / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D013481:Superoxides; D008353:Mannitol; D002374:Catalase; D013482:Superoxide Dismutase