title: Analysis of transforming gene regions of human papillomavirus type 16 in normal cervical smears.
abstract: Exfoliated cells from the uterine cervix of 102 Hungarian women with no cytological abnormality were screened using the polymerase chain reaction (PCR) for human papillomavirus (HPV) type 16 infection. Twenty-nine patients with histologically confirmed cervical intraepithelial neoplasia (CIN) served as reference cases. PCR was performed with 2 different HPV 16 specific oligonucleotide primer pairs flanking a 300 and a 200 base-pair fragment from the early 6 (E6) and early 7 (E7) genes, position 215-514 and 605-805. The specimens exhibited the same proportions of type 16 sequences specific for the tested regions. 8.8% (9/102) of normal samples showed amplification for HPV type 16 E6 and E7 regions, while 48.3% (14/29) of CIN biopsies were positive for the same gene sequences.
authors.affiliation: Institute of Microbiology, University Medical School, Debrecen, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Czeglédy
authors.forename: I
authors.initials: I
authors.lastname: Batár
authors.forename: M
authors.initials: M
authors.lastname: Evander
authors.forename: L
authors.initials: L
authors.lastname: Gergely
authors.forename: G
authors.initials: G
authors.lastname: Wadell
mesh_terms: D002584:Cervix Uteri / Q000473:pathology; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics; D009857:Oncogenes / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; D014412:Tumor Virus Infections / Q000235:genetics* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology; D014626:Vaginal Smears
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16