title: The interaction between HPV infection and estrogen metabolism in cervical carcinogenesis.
abstract: Cancer of the genital tract is the final outcome of some infections with human papillomavirus (HPVs), and the most estrogen-sensitive cells are at greatest risk for the HPV-related cancers. Therefore we investigated relationships between HPVs and estrogen metabolism in cells of the genital tract. Increased conversion of estradiol to 16 alpha-hydroxyestrone, known to be a risk factor for cancer in some other estrogen-sensitive cells, was investigated in keratinocytes from the genital tract. Primary cells, particularly those explants from the transformation zone of the cervix, are able to 16 alpha-hydroxylate estradiol. Both cervical and foreskin cells immortalized with HPV-16 are greatly enhanced in the 16 alpha-hydroxylation of estradiol as compared with normal cells. We suggest a model whereby the combined action of 16 alpha-hydroxylation of estrogen and HPV work together to promote cell proliferation.
authors.affiliation: Department of Otolaryngology, Long Island Jewish Medical Center, New Hyde Park, New York 11042.
authors.forename: K J
authors.initials: KJ
authors.lastname: Auborn
authors.forename: C
authors.initials: C
authors.lastname: Woodworth
authors.forename: J A
authors.initials: JA
authors.lastname: DiPaolo
authors.forename: H L
authors.initials: HL
authors.lastname: Bradlow
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases; D001711:Biotransformation; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000378:metabolism; D065727:Cytochrome P-450 CYP2C8; D065729:Cytochrome P-450 CYP2C9; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D008297:Male; D027383:Papillomaviridae / Q000235:genetics; D039181:Steroid 16-alpha-Hydroxylase; D014412:Tumor Virus Infections / Q000150:complications*; D002583:Uterine Cervical Neoplasms / Q000209:etiology*
chemical_list: D004967:Estrogens; C450260:CYP2C9 protein, human; D065729:Cytochrome P-450 CYP2C9; D001189:Aryl Hydrocarbon Hydroxylases; C450259:CYP2C8 protein, human; D065727:Cytochrome P-450 CYP2C8; D039181:Steroid 16-alpha-Hydroxylase