The outer membrane permeability-increasing action of deacylpolymyxins.
The outer membrane permeability-increasing action of deacylpolymyxins was compared to the well-known potent action of polymyxin B nonapeptide (PMBN). Deacylpolymyxin B (DAPB), prepared by treating polymyxin B with polymyxin acylase, was found to be a slightly more effective permeabilizer than PMBN. As low a DAPB concentration as 1 microgram/ml sensitized Escherichia coli to the probe antibiotics (rifampin, fusidic acid, erythromycin, clindamycin, novobiocin) by factors 30-100 and Salmonella typhimurium by factors 10-100. A higher concentration (3 micrograms/ml) of DAPB elicited further sensitization. Also deacylcolistin (DAC) was found to be an effective permeabilizer.
Viljanen
P
P
Department of Fisheries, Faculty of Agriculture, Miyazaki University, Japan.
Matsunaga
H
H
Kimura
Y
Y
Vaara
M
M
D000595:Amino Acid Sequence; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects; D008969:Molecular Sequence Data; D011112:Polymyxin B / Q000031:analogs & derivatives / Q000494:pharmacology; D011113:Polymyxins / Q000138:chemical synthesis / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects
D011113:Polymyxins; C069640:deacylpolymyxin B; C038934:polymyxin B nonapeptide; D011112:Polymyxin B