title: Enhancer effect of bovine papillomavirus E2 protein in replication of polyomavirus DNA.
abstract: In polyomavirus, both transcription from the early promoter and viral DNA replication initiated at the origin of DNA replication is controlled by binding of proteins to the enhancer region. We have developed a simple model system to study the role of an enhancer binding factor in the initiation of polyomavirus DNA replication. A reporter plasmid was constructed which has the enhancer region replaced by two binding sites for a transcription factor, the E2 protein encoded by bovine papillomavirus type 1. Co-transfection of COP 5 cells which express polyomavirus large T-antigen, with the reporter plasmid and an E2 expression plasmid, resulted in E2-dependent stimulation of replication of the reporter plasmid. The activity of several E2 mutants was also analysed. Mutant proteins with decreased activity in transcriptional trans-activation, resulting from impaired DNA binding or other defects, were found to have quantitatively similar reductions of activity in viral DNA replication. The results suggest that the E2 protein activates transcription and polyomavirus DNA replication by similar mechanism(s).
authors.affiliation: Department of Medical Virology, Uppsala University, Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Nilsson
authors.forename: M
authors.initials: M
authors.lastname: Forsberg
authors.forename: Z Y
authors.initials: ZY
authors.lastname: You
authors.forename: G
authors.initials: G
authors.lastname: Westin
authors.forename: G
authors.initials: G
authors.lastname: Magnusson
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002461:Cell Line, Transformed; D004261:DNA Replication / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C051550:E2 protein, Bovine papillomavirus; D015534:Trans-Activators; D014764:Viral Proteins