title: Clinical utility of fractionating erythrocytes into "Percoll" density gradients.
abstract: Two rapid methods for fractionating the RBC into five or nine layers of increasing density are reported. These procedures have been used to monitor the decline of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activity during the process of red cell aging in normal subjects and in beta-thal carriers, to study transfused patients with G6PD and pyruvate kinase (PK) deficiency and to test the effects of inositol hexaphosphate (IHP) encapsulation on RBC subpopulations.
authors.affiliation: Dipt. Sc. Tecnol. Biomediche, Univ. Milano.
authors.forename: A
authors.initials: A
authors.lastname: Mosca
authors.forename: R
authors.initials: R
authors.lastname: Paleari
authors.forename: A
authors.initials: A
authors.lastname: Modenese
authors.forename: S
authors.initials: S
authors.lastname: Rossini
authors.forename: R
authors.initials: R
authors.lastname: Parma
authors.forename: C
authors.initials: C
authors.lastname: Rocco
authors.forename: V
authors.initials: V
authors.lastname: Russo
authors.forename: G
authors.initials: G
authors.lastname: Caramenti
authors.forename: M L
authors.initials: ML
authors.lastname: Paderi
authors.forename: R
authors.initials: R
authors.lastname: Galanello
mesh_terms: D000328:Adult; D000743:Anemia, Hemolytic / Q000097:blood; D002458:Cell Fractionation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D002648:Child; D004796:Clinical Enzyme Tests; D004339:Drug Compounding; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000097:blood*; D006579:Heterozygote; D006593:Hexokinase / Q000097:blood; D006801:Humans; D008297:Male; D010734:Phosphogluconate Dehydrogenase / Q000097:blood*; D010833:Phytic Acid / Q000008:administration & dosage; D011205:Povidone; D011770:Pyruvate Kinase / Q000097:blood / Q000172:deficiency*; D012156:Reticulocytes / Q000201:enzymology; D012822:Silicon Dioxide; D013789:Thalassemia / Q000097:blood*
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D010833:Phytic Acid; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D011770:Pyruvate Kinase; D005634:Fructose-Bisphosphate Aldolase; D011205:Povidone