Radiation-induced structural alterations in fish red blood cells.
The structural properties of gamma-irradiated fish red blood cells were studied using a spin labelling method. The gradient increase of lipid fluidity with the increasing gamma radiation doses was indicated by methyl 5-doxylpalmitate and methyl 12-doxylstearate spin labels spectra. In turns, the spectra of maleimide spin label (4-maleimido-2,2,6,6-tetramethylpiperidine-1-oxyl) and TEMPONE (4-oxo-2,2,6,6-tetramethylpiperidine-1-oxyl) indicated a modification of the internal proteins and the increased internal viscosity of red blood cells. The results encourage the conclusion that the increase in membrane fluidity may result from the alternations in lipid-protein interactions rather than lipid peroxidation.
Gwoździński
K
K
Department of Biophysics, University of Lódź, Poland.
D000818:Animals; D002347:Carps; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000737:chemistry / Q000528:radiation effects*; D005609:Free Radicals; D005720:Gamma Rays; D015227:Lipid Peroxidation; D013113:Spin Labels; D014783:Viscosity
D005609:Free Radicals; D013113:Spin Labels