Localization of nerve growth factor receptor mRNA in contused rat spinal cord by in situ hybridization.
Northern blot analysis using a probe for the low-affinity nerve growth factor receptor (NGFR) revealed that a mild contusive injury induces the expression of NGFR mRNA in rat spinal cord with a maximal expression at 7 days post-injury. We have now localized this induction using in situ hybridization and found the highest concentration of NGFR mRNA at the lesion epicenter. The location and pattern of autoradiographic grains were compared with that of various cell types at the injury site as determined by immunocytochemical studies. The results suggest that cells associated with blood vessels at the epicenter are induced to express NGFR mRNA at 7 days post-injury.
Reynolds
M E
ME
Department of Anatomy and Cell Biology, Georgetown University, Washington, DC 20007.
Brunello
N
N
Mocchetti
I
I
Wrathall
J R
JR
D000818:Animals; D015152:Blotting, Northern; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D007150:Immunohistochemistry; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D017475:Receptors, Nerve Growth Factor; D013119:Spinal Cord Injuries / Q000378:metabolism*; D013462:Sulfur Radioisotopes
D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D013462:Sulfur Radioisotopes