[Construction and expression of hybrid genes based on a deletion mutant of the herpes virus thymidine kinase gene in Escherichia coli].
The plasmids with the deletion mutant of thymidine kinase gene (tk') of Herpes simplex virus were constructed. The promoter, transcription initiation site and first 35 codons of tk gene were removed and replaced with a series of DNA restriction sites. The DNA fragments, containing the gene regulatory sequences specific for bacteria, were cloned into these sites and shown to express enzymatically active proteins. The obtained ene fusions were able to complement in E. coli strain deficient in thymidine kinase function. Such plasmid vectors carrying tk' are useful for construction and selection of hybrid gene fusions.
Zemskova
M Iu
MIu
Dzhavakhishvili
Ts D
TsD
Bukhtiiarova
M G
MG
Berezina
Iu V
IuV
Fodor
I I
II
D001345:Autoradiography; D001483:Base Sequence; D002872:Chromosome Deletion; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D015967:Gene Expression Regulation, Viral; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000235:genetics*; D014170:Transformation, Genetic
D013937:Thymidine Kinase
Konstruirovanie i ékspressiia gibridnykh genov na osnove deletsionnogomutanta gena timidinkinazy virusa gerpesa v kletkakh Escherichia coli.