Isolation of type I and type II GABAA-benzodiazepine receptors by immunoaffinity chromatography.
Anti-peptide alpha 1 (1-9) and anti-peptide alpha 3 (459-467) antibodies coupled to Affigel-10 were used for the isolation of GABAA receptors containing the alpha 1- or alpha 3-subunit, respectively. Both types of GABAA receptors exhibited a high affinity for [3H]flunitrazepam, and binding of [3H]flunitrazepam was stimulated in the presence of GABA. GABAA receptors eluted from the anti-peptide alpha 1 (1-9) immunoaffinity column exhibited a high affinity and those from the anti-peptide alpha 3 (459-467) columns a low affinity for the type I benzodiazepine receptor-selective ligand Cl 218872, indicating the enrichment of type I and type II GABAA-benzodiazepine receptors, respectively.
Zezula
J
J
Department of Biochemical Psychiatry, Psychiatrische Universitätsklinik, Vienna, Austria.
Sieghart
W
W
D000818:Animals; D001921:Brain / Q000276:immunology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D010446:Peptide Fragments / Q000276:immunology; D051381:Rats; D011963:Receptors, GABA-A / Q000276:immunology / Q000302:isolation & purification*
D010446:Peptide Fragments; D011963:Receptors, GABA-A