title: Fast PCR based therapeutic follow-up of T-cell malignancies.
abstract: Available methods for the detection of minimal residual disease in T-cell malignancies are limited by their poor sensitivity and/or by their complexity. With the aim of avoiding these drawbacks, we used the Fast PCR technique in order to amplify V delta 1-(D delta 1)-(D delta 2)-J delta 1 and V gamma I family-J gamma junctional sequences from nucleated cells of boiled bone marrow. We were thus able to detect malignant T-cells down to a dilution of 1 in 665 nucleated marrow cells, in less than 4 hours after sampling. This new quantitative method is promising for monitoring therapy and detecting early disease relapse in T-lymphoproliferative disease, since it is 2 to 35 fold more sensitive than Southern blotting.
authors.affiliation: Virology Department, Brugmann University Hospital, Brussels, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Vandenvelde
authors.forename: R
authors.initials: R
authors.lastname: Scheen
authors.forename: M
authors.initials: M
authors.lastname: Duys
authors.forename: F
authors.initials: F
authors.lastname: Corazza
authors.forename: D
authors.initials: D
authors.lastname: Van Beers
mesh_terms: D001483:Base Sequence; D001856:Bone Marrow Examination / Q000379:methods*; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000235:genetics; D005500:Follow-Up Studies; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D007938:Leukemia / Q000235:genetics; D015458:Leukemia, T-Cell / Q000235:genetics* / Q000473:pathology; D008223:Lymphoma; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000235:genetics; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D004273:DNA, Neoplasm