Suitability of polyethylene glycol for precipitation of monoclonal antibodies--a radiometric study.
125I labelled monoclonal antibody (MoAb) to thyroid stimulating hormone (TSH) was used as a tracer for assessing the suitability of polyethylene glycol (PEG) for precipitation of MoAb and MoAb-antigen (AG) complexes. It was found that PEG can be advantageously used under certain conditions favouring insolubility and could thus be a non-specific separation reagent for radioimmunoassays based on MoAbs.
Rathinaswamy
A
A
Radiopharmaceuticals Operations, Board of Radiation and Isotope Technology, New Bombay, India.
Chatur
J
J
Nagvekar
U H
UH
Ramamoorthy
N
N
Desai
C N
CN
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex; D011232:Chemical Precipitation; D007118:Immunoassay / Q000379:methods; D007457:Iodine Radioisotopes; D011092:Polyethylene Glycols
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007457:Iodine Radioisotopes; D011092:Polyethylene Glycols