Transposition of Tn5096 from a temperature-sensitive transducible plasmid in Streptomyces spp.
Transposon Tn5096 was inserted into a derivative of the temperature-sensitive plasmid pMT660 containing the bacteriophage FP43 pac site. The resulting plasmid, pRHB126, was transduced by FP43 into several Streptomyces species. Tn5096 transposed from pRHB126 into different sites in the genomes of Streptomyces ambofaciens, Streptomyces cinnamonensis, Streptomyces coelicolor A3(2), Streptomyces fradiae, Streptomyces griseofuscus, and Streptomyces thermotolerans.
McHenney
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Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
Baltz
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D001435:Bacteriophages / Q000235:genetics; D015139:Blotting, Southern; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005814:Genes, Viral; D010957:Plasmids; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics*; D013696:Temperature; D014161:Transduction, Genetic
D004251:DNA Transposable Elements; D004269:DNA, Bacterial