HPV detection by polymerase chain reaction (PCR) in verrucous lesions of the upper aerodigestive tract.
Nineteen formalin-fixed, paraffin-embedded verrucous lesions of the upper aerodigestive tract (UADT) were evaluated for the presence of human papillomavirus (HPV) 6b/11, 16, and 18 DNA sequences using the polymerase chain reaction (PCR), in-situ hybridization, and dot blot analysis. HPV DNA was confirmed in two dysplastic papillomas only; both cases contained HPV 6b/11. E6-E7 portions of HPV DNA was not reproducibly detected in any of the 11 verrucous carcinomas, 4 verrucous hyperplasias, or 2 mature papillomas. In-situ hybridization and dot blot analysis confirmed HPV 6b/11 in the two dysplastic papillomas and failed to identify HPV in the other verrucous lesions.
Johnson
T L
TL
Department of Pathology, Henry Ford Hospital, Detroit, Michigan 48202.
Plieth
D A
DA
Crissman
J D
JD
Sarkar
F H
FH
D000328:Adult; D000368:Aged; D001483:Base Sequence; D002291:Carcinoma, Papillary / Q000382:microbiology*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000235:genetics; D004064:Digestive System / Q000382:microbiology* / Q000473:pathology; D004066:Digestive System Diseases / Q000382:microbiology* / Q000473:pathology; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007822:Laryngeal Neoplasms / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000382:microbiology; D009062:Mouth Neoplasms; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
D015344:DNA Probes, HPV; D004279:DNA, Viral