[Effects of verapamil on prostacyclin, thromboxane and pancreatic blood flow of rats with experimental acute pancreatitis].
We studied the effects of the calcium channel blocker, verapamil on experimental acute pancreatitis (AP) in rats. The pancreatic blood flow (PBF) and pancreatic tissue perfusion (PTP) were measured after induction of AP. At the same time, the plasma levels of TXB2 and 6-keto-PGF1 alpha were determined by radioimmunoassay. The survival rate and the mean survival time after induction of AP were determined and the pancreatic histology was examined by light and electron microscopy. The results demonstrated a significant early fall in PBF and PTP, a marked increase in TXB2 levels and a slight increase in 6-keto-PGF1 alpha after induction of AP. Treatment with 0.1 mg/100 g verapamil significantly increased PBF and PTP, and decreased TXB2, resulting in a normalization of TXB2/6-keto-PGF1 alpha ratio. The treated animals also exhibited significant increase in the survival rate and the survival time. They also showed decrease in the severity of pancreatic hemorrhage and necrosis, and in the damage to the cellular ultrastructures. These data suggest that calcium blockade increases PBF and PTP, limits the prostanoid imbalance in the early phase of AP, and may influence the development of experimental acute pancreatitis in rats.
Shen
J
J
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood*; D000208:Acute Disease; D000818:Animals; D005260:Female; D008297:Male; D010179:Pancreas / Q000098:blood supply*; D010195:Pancreatitis / Q000188:drug therapy / Q000503:physiopathology*; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow / Q000187:drug effects; D013929:Thromboxane B2 / Q000097:blood*; D014700:Verapamil / Q000494:pharmacology* / Q000627:therapeutic use
D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D014700:Verapamil