Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase.
The nucleotide sequence of the ERG12 gene, encoding mevalonate kinase, from Saccharomyces cerevisiae is presented. The longest open reading frame may code for a protein containing 443 amino acids with a deduced relative molecular mass of 48,500. The analysis of the nucleotide sequence reveals a complete identity with the yeast gene RAR1, isolated elsewhere by complementation of a rar1 mutation involved in the stability of plasmids with weak ARS. In addition, we show that mevalonate kinase is not a rate-limiting enzyme; however its sensitivity to FFP could be a key regulatory mechanism in the sterol pathway of yeast.
Oulmouden
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Laboratoire de Biochimie et Génétique des Microorganismes, Université de Poitiers, France.
Karst
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F
D000595:Amino Acid Sequence; D001483:Base Sequence; D004875:Ergosterol / Q000096:biosynthesis; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D011106:Polyisoprenyl Phosphates / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012717:Sesquiterpenes
D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; C015234:geranyl pyrophosphate; C004808:farnesyl pyrophosphate; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021796:mevalonate kinase; D004875:Ergosterol