A hybrid form of alkaline phosphatase produced in FL-amnion cells.
FL-amnion cells have mainly two alkaline phosphatase (AP) isozymes, of which the faster migrating one (FL-APF) on 5% polyacrylamide gel electrophoresis has proved to be identical to the Kasahara isozyme, a tumor-associated AP of intestinal type. But the other slower migrating one (FL-APs) remains to be characterized. Immunological and enzymic examination of purified FL-APs revealed that it is a hybrid form of AP consisting of one subunit with a molecular weight of 66,200 coming from the same subunit as that of FL-APF and another one with a molecular weight of 58,000 from placental AP subunit with modified glycosylation.
Hada
T
T
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
Imanishi
H
H
Muratani
K
K
Hirano
K
K
Higashino
K
K
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D000650:Amnion / Q000201:enzymology*; D006528:Carcinoma, Hepatocellular / Q000201:enzymology; D002460:Cell Line; D006801:Humans; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000302:isolation & purification*; D008113:Liver Neoplasms / Q000201:enzymology; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology
D007527:Isoenzymes; D000469:Alkaline Phosphatase