Detection of Epstein-Barr virus in lymphoepithelioma-like carcinoma of the lung.
A case of lymphoepithelioma-like carcinoma of the lung is reported in a Chinese patient with serologic evidence of previous Epstein-Barr (EBV) infection. EBV genomes were detected in the tumor using polymerase chain reaction (PCR) and in situ hybridization (ISH) from formalin-fixed, paraffin-embedded tissue blocks. The use of both techniques afforded the exquisite sensitivity of PCR to detect a few copies of EBV DNA with the ability of ISH to precisely localize EBV to the carcinoma, but not to the lymphoid stroma. These findings support a potential oncogenic relationship between EBV and lymphoepithelioma-like carcinoma of the lung.
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Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia.
Unger
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Koss
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Yen
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D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000382:microbiology* / Q000473:pathology; D004279:DNA, Viral / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008175:Lung Neoplasms / Q000382:microbiology* / Q000473:pathology; D008297:Male; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D004279:DNA, Viral