A simple, rapid immunoassay for the detection of simian immunodeficiency virus antibodies.
Detection of simian immunodeficiency virus (SIV) antibodies has proved useful in a wide variety of research studies. Conventional immunoassays, however, are difficult to perform outside the well-equipped laboratory or under field conditions. We have developed an inexpensive, simple, rapid immunoassay for the detection of SIV antibodies that utilizes inactivated SIV antigen and Fast-Chek (F-C) (E.Y. Laboratories, San Mateo, Ca)., which is a membrane/filter paper device that uses protein A gold to detect antibody and/or antigen. This low-cost 10-min assay requires minimal technical skill and no refrigeration, electrical power, or sophisticated laboratory equipment. In a study of 155 banked sera, from a number of monkey species in a variety of geographic locations, F-C and Western immunoblot result concordance was 96%. Relative sensitivity and specificity were 98% and 95%, respectively.
Yee
J L
JL
Department of Medical Pathology, University of California, Davis.
Jennings
M B
MB
Carlson
J R
JR
Lerche
N W
NW
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D001426:Bacterial Proteins; D015153:Blotting, Western; D016665:Cercocebus atys; D006046:Gold; D017979:Gold Colloid; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007124:Immunoenzyme Techniques; D008253:Macaca mulatta; D009942:Organometallic Compounds; D011933:Reagent Kits, Diagnostic; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013205:Staphylococcal Protein A
D000914:Antibodies, Viral; D000956:Antigens, Viral; D001426:Bacterial Proteins; D017979:Gold Colloid; D009942:Organometallic Compounds; D011933:Reagent Kits, Diagnostic; D013205:Staphylococcal Protein A; C070001:protein AG-gold complex; D006046:Gold