Characterization of a neuronal interleukin-1 receptor and the corresponding mRNA in the mouse anterior pituitary cell line AtT-20.
Interleukin-1 (IL-1) mediates numerous responses on the mouse anterior pituitary cell line AtT-20. We have studied the ligand binding properties of the IL-1 receptors (IL-1R) of the AtT-20 cells and found that they possess 1.5 x 10(3) receptors/cell with a Kd of 0.15 nM for [125I]IL-1 alpha. Using oligonucleotide primers, which define a 236 bp region of the cDNA coding for the cytosolic part of the mouse T-cell IL-1R, and cDNA prepared from AtT-20 cells, we obtained by polymerase chain reaction (PCR) a DNA fragment which was shown to possess an identical sequence to that of the corresponding region of the mouse T-cell IL-1R. Thus the AtT-20 pituitary cell line IL-1 receptor appears to be similar to those found on mouse T-cells and fibroblasts.
Bristulf
J
J
Department of Biochemistry, Arrheniuslaboratory, Stockholm University, Sweden.
Simoncsits
A
A
Bartfai
T
T
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002460:Cell Line; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000032:analysis / Q000378:metabolism*; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins / Q000032:analysis; D013601:T-Lymphocytes / Q000378:metabolism
D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D000324:Adrenocorticotropic Hormone; D004247:DNA