title: Production, isolation and characterization of human profilin from Saccharomyces cerevisiae.
abstract: The yeast Saccharomyces cerevisiae was used to express human profilin cDNA. The recombinant protein, isolated by affinity chromatography on poly(L-proline)-Sepharose followed by ion exchange chromatography, associates with non-muscle actin and phosphatidylinositol-(4,5)-bisphosphate as authentic profilin.
authors.affiliation: Department of Developmental Biology, Uppsala University, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Aspenström
authors.forename: I
authors.initials: I
authors.lastname: Lassing
authors.forename: R
authors.initials: R
authors.lastname: Karlsson
mesh_terms: D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D003285:Contractile Proteins; D004247:DNA / Q000235:genetics; D008840:Microfilament Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000378:metabolism; D051304:Profilins; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D000199:Actins; D003285:Contractile Proteins; D008840:Microfilament Proteins; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D051304:Profilins; D011993:Recombinant Fusion Proteins; D004247:DNA