Effects of some gastrointestinal peptides on isolated human and rabbit gastric glands.
The isolated gastric gland preparation, with aminopyrine accumulation as an index of the parietal cell response, has been used to study the effects of somatostatin (S-14), gastrin-releasing peptide (GRP), cholecystokinin (CCK-8), vasoactive intestinal peptide (VIP), and peptide YY (PYY) on the in vitro acid secretion in human and rabbit oxyntic mucosa. Somatostatin was able to inhibit the parietal cell response to histamine in both human and rabbit isolated gastric glands (maximal inhibition, 22% and 34%, respectively) but failed to inhibit the parietal cell response to db-cAMP. However, other peptides capable of inhibiting gastric acid secretion in vivo, such as CCK, VIP, and PYY, were unable to induce any inhibition of the parietal cell response to db-cAMP or histamine in the isolated gastric gland preparation irrespective of the species studied. GRP was not able to induce a parietal cell response, a finding that is in accord with the assumption that the stimulatory effect of GRP on gastric acid secretion in vivo is by releasing gastrin from antral G-cells.
Leth
R
R
Dept. of Surgery, Sahlgren's Hospital, Gothenburg University, Sweden.
Lundell
L
L
Olbe
L
L
D000818:Animals; D002766:Cholecystokinin / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism; D019886:Gastrin-Releasing Peptide; D005768:Gastrointestinal Hormones / Q000494:pharmacology; D006801:Humans; D010295:Parietal Cells, Gastric / Q000187:drug effects* / Q000378:metabolism; D019894:Peptide YY; D010455:Peptides / Q000494:pharmacology; D011817:Rabbits; D013004:Somatostatin / Q000378:metabolism / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D005768:Gastrointestinal Hormones; D010455:Peptides; D019894:Peptide YY; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D019886:Gastrin-Releasing Peptide; D002766:Cholecystokinin