Direct serotyping of porcine rotaviruses using VP7-specific monoclonal antibodies by an enzyme immunoassay.
Employing a serotyping EIA test using MAbs both cell culture adapted and faecal porcine rotaviruses were classified into serotypes G3, G3/5, G4, and G5. The MAbs have confirmed and extended the serotyping results obtained using polyclonal antisera. These MAbs are therefore potential reagents for serotyping of porcine rotaviruses. Using subgroup specific MAbs serotypes G3, G3/5, and G5 were found to contain subgroup I antigens while G4 rotaviruses contained either subgroup II or subgroup I antigens.
Nagesha
H S
HS
School of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
Holmes
I H
IH
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000918:Antibody Specificity; D000956:Antigens, Viral; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D005759:Gastroenteritis / Q000382:microbiology / Q000662:veterinary; D007124:Immunoenzyme Techniques; D012401:Rotavirus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000382:microbiology / Q000662:veterinary; D012703:Serotyping / Q000379:methods*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus