title: Glutamine synthetase in oligodendrocytes and astrocytes: new biochemical and immunocytochemical evidence.
abstract: The results of recent immunocytochemical experiments suggest that glutamine synthetase (GS) in the rat CNS may not be confined to astrocytes. In the present study, GS activity was assayed in oligodendrocytes isolated from bovine brain and in oligodendrocytes, astrocytes, and neurons isolated from rat forebrain, and the results were compared with new immunochemical data. Among the cells isolated from rat brain, astrocytes had the highest specific activities of GS, followed by oligodendrocytes. Oligodendrocytes isolated from white matter of bovine brain had GS specific activities almost fivefold higher than those in white matter homogenates. Immunocytochemical staining also showed the presence of GS in both oligodendrocytes and astrocytes in bovine forebrain, in three white-matter regions of rat brain, and in Vibratome sections as well as paraffin sections.
authors.affiliation: Department of Neurology, Albert Einstein College of Medicine, Bronx, New York 10461.
authors.forename: F A
authors.initials: FA
authors.lastname: Tansey
authors.forename: M
authors.initials: M
authors.lastname: Farooq
authors.forename: W
authors.initials: W
authors.lastname: Cammer
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D001921:Brain / Q000166:cytology; D002417:Cattle; D004027:Diencephalon / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D007150:Immunohistochemistry; D009474:Neurons / Q000201:enzymology*; D009836:Oligodendroglia / Q000201:enzymology*; D051381:Rats; D013687:Telencephalon / Q000201:enzymology
chemical_list: D005904:Glial Fibrillary Acidic Protein; D005974:Glutamate-Ammonia Ligase