title: Localization of 616 human chromosome 3-specific cosmids using a somatic cell hybrid deletion mapping panel.
abstract: A total of 5700 human chromosome 3-specific cosmid clones was isolated from a series of cosmid libraries constructed from somatic cell hybrids whose only human component was an entire chromosome 3 or a chromosome 3 containing an interstitial deletion removing 50% of long arm sequences. Several unique sequence chromosome 3-specific hybridization probes were isolated from each of 616 of these cosmids. These probes were then used to localize the cosmids by hybridization to a somatic cell hybrid deletion mapping panel capable of resolving chromosome 3 into nine distinct subregions. All 616 of the cosmids were localized to either the long or short arm of chromosome 3 and 63% of the short arm cosmids were more precisely localized. We have identified a total of 87 cosmids that contain fragments that are evolutionarily conserved. Fragments from these cosmids should prove useful in the identification of new chromosome 3-specific genes as well as in comparative mapping studies. The localized cosmids should provide excellent saturation of human chromosome 3 and facilitate the construction of physical and genetic linkage maps to identify various disease loci including Von Hippel Lindau disease and renal and small cell lung carcinoma.
authors.affiliation: Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, Michigan 48201.
authors.forename: D I
authors.initials: DI
authors.lastname: Smith
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Ginzinger
authors.forename: P
authors.initials: P
authors.lastname: Green
authors.forename: S
authors.initials: S
authors.lastname: Smith
authors.forename: N D
authors.initials: ND
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Recchia
authors.forename: K
authors.initials: K
authors.lastname: Carolyn
authors.forename: H
authors.initials: H
authors.lastname: Drabkin
authors.forename: W
authors.initials: W
authors.lastname: Golembieski
mesh_terms: D000818:Animals; D016466:CHO Cells; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D003360:Cosmids; D006224:Cricetinae; D015342:DNA Probes; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015698:Genomic Library; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic
chemical_list: D015342:DNA Probes; D004262:DNA Restriction Enzymes