Posttranscriptional regulation of beta interferon expression in erythroid Friend cells treated with gamma interferon.
Treatment of Friend erythroleukemia cells (FLC) with gamma interferon (IFN-gamma) in the presence of anti-IFN-beta antibodies reduces the effectiveness of the antiviral state and the induction of 2'-5'-oligoadenylate synthetase activity, indicating that the antiviral activity of IFN-gamma in FLC is in part mediated by the production of IFN-beta. Accordingly, IFN-gamma induces a less pronounced antiviral state in FLC resistant to IFN-alpha/beta than in wild-type cells. Moreover, while results of run-on assays indicate that both IFN-alpha and -beta genes are constitutively transcribed in these cells, FLC treatment with IFN-gamma induces only IFN-beta mRNA accumulation. These results indicate that posttranscriptional mechanisms are involved in the regulation of IFN-beta and -alpha expression by IFN-gamma. The low amounts of the induced IFN-beta synergize with IFN-gamma in mounting the potent antiviral effect.
Marziali
G
G
Laboratorio di Virologia, Istituto Superiore di Sanità, Rome, Italy.
Fiorucci
G
G
Coccia
E M
EM
Percario
Z
Z
Raber
J
J
Battistini
A
A
Rossi
G B
GB
Affabris
E
E
Romeo
G
G
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004686:Encephalomyocarditis virus / Q000276:immunology; D004790:Enzyme Induction; D005622:Friend murine leukemia virus; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology*; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology*; D009693:Nucleic Acid Hybridization; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology
D007370:Interferon Type I; D012333:RNA, Messenger; D012367:RNA, Viral; D007371:Interferon-gamma; D015088:2',5'-Oligoadenylate Synthetase