Human trophoblastic cells metabolize 25-hydroxyvitamin D3 in vitro.
Human trophoblastic cells (T3M-3) metabolized 25-hydroxyvitamin D3 into two more polar metabolites during in vitro incubations. A two-step high pressure liquid chromatography system revealed two unique elution positions of those trophoblastic cell-derived metabolites that exactly co-migrated with the elution positions of 5(Z)-19-nor-10-oxo-25-hydroxyvitamin D3 and 5(E)-19-nor-10-oxo-25-hydroxyvitamin D3, respectively. These unique metabolites did not bind specifically to a protein receptor for 1,25-dihydroxyvitamin D3.
Okabe
T
T
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Ishizaka
S
S
Fujisawa
M
M
D002112:Calcifediol / Q000031:analogs & derivatives / Q000378:metabolism*; D002822:Choriocarcinoma / Q000378:metabolism; D005260:Female; D006801:Humans; D011247:Pregnancy; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014594:Uterine Neoplasms / Q000378:metabolism
D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D002112:Calcifediol