title: Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesicles.
abstract: We recently developed a procedure for immunoisolating insulin-responsive membrane vesicles that contain the muscle/fat glucose transporter isoform, GLUT 4, from rat adipocytes. Utilizing this methodology, we are analyzing the components of these vesicles to gain an understanding of how they are regulated by insulin. In this report we identify a phosphatidylinositol (PtdIns) 4-kinase as a constituent of glucose transporter vesicles (GTVs). This kinase has the biochemical and immunological properties of a type II PtdIns 4-kinase as classified by Endeman et al. (Endemann, G., Dunn, S. N., and Cantley, L. C. (1987) Biochemistry 26, 6845-6852). A monoclonal antibody, 4C5G, which specifically inhibits the type II PtdIns 4-kinase, suppresses 80% of the GTV-PtdIns 4-kinase activity. In addition, the GTVs-PtdIns 4-kinase is maximally activated by the nonionic detergent Triton X-100, at a concentration of 0.2% and is inhibited by adenosine with a Ki of approximately 20-30 microM. We find that the GTVs do not contain any PtdIns4P 5-kinase or diacylglycerol kinase activities, whereas these activities were detected in the plasma membrane. An analysis of the subcellular distribution of PtdIns 4-kinase activity in the rat adipocyte shows that there are similar levels of activity in GTVs, plasma membranes, and the high and low density microsomal fractions, whereas the mitochondria- and nuclei-containing fractions have less than 5% of the activity seen in other fractions. Low density microsomes were subfractionated by sucrose density gradient centrifugation and PtdIns 4-kinase activity was found to correlate closely with the distribution of membrane protein, indicating that the activity is equally distributed throughout this heterogenous population of membranes. PtdIns 4-kinase activity measured in GTVs, plasma membranes, and low density microsomes, was not affected by prior treatment of the intact adipocytes with 35 nM insulin. We postulate that while the GTV-PtdIns 4-kinase is not regulated by insulin, it may play a role in defining the fusogenic properties necessary to mediate membrane movement between the GTVs, plasma membranes, and microsomes.
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118.
authors.forename: R L
authors.initials: RL
authors.lastname: Del Vecchio
authors.forename: P F
authors.initials: PF
authors.lastname: Pilch
mesh_terms: D019870:1-Phosphatidylinositol 4-Kinase; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D002501:Centrifugation, Zonal; D019852:Diacylglycerol Kinase; D007328:Insulin / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000378:metabolism / Q000648:ultrastructure; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins / Q000302:isolation & purification* / Q000378:metabolism; D010770:Phosphotransferases / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007328:Insulin; D009004:Monosaccharide Transport Proteins; D010770:Phosphotransferases; D019852:Diacylglycerol Kinase; D019870:1-Phosphatidylinositol 4-Kinase