An investigation on the quinoprotein nature of some fungal and plant oxidoreductases.
The presence of pyrroloquinoline quinone (PQQ) as the organic cofactor of Dactylium dendroides galactose oxidase and lentil (Lens culinaris) seedling amine oxidase, purported PQQ-containing oxidoreductases (Van der Meer, R. A., Jongejan, J. A., and Duine, J. A. (1989) J. Biol. Chem. 264, 7792-7794; Citro, G., Verdina, A., Galati, R., Floris, G., Sabatini, S., and Finazzi-Argo', A. (1989) FEBS Lett. 247, 201-204), was reinvestigated using the nitro blue tetrazolium redoxcycling method (Paz, M. A., Gallop, P. M., Torrelio, B. M., and Flückiger, R. (1988) Biochem. Biophys. Res. Commun. 154, 1330-1337; Paz, M. A., Flückiger, R., Boak, A., Kagan, H. M., and Gallop, P. M. (1991) J. Biol. Chem. 266, 689-692) and the enzyme-linked immunosorbent assay with polyclonal antibodies against PQQ. The possible quinoprotein nature of the laccases from Polyporus versicolor and Rhus vernicifera was also investigated because of the similarities in spectroscopic and kinetic features of these enzymes and the laccase from Phlebia radiata, reported to be a PQQ protein (Karhunen, E., Niku-Paavola, M.-L., Viikari, L., Haltia, T., Van der Meer, R. A., and Duine, J. A. (1990) FEBS Lett. 267, 6-8). The presence of a quinonoid cofactor in lentil seedling amine oxidase is confirmed, whereas galactose oxidase and both laccases do not display any quinoprotein nature.
Maccarrone
M
M
Bijvoet Center for Biomolecular Research, Department of Bio-organic Chemistry, Utrecht University, The Netherlands.
Veldink
G A
GA
Vliegenthart
J F
JF
D006631:Amine Oxidase (Copper-Containing); D003067:Coenzymes / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D005692:Galactose Oxidase / Q000737:chemistry*; D042845:Laccase; D010088:Oxidoreductases / Q000737:chemistry*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000737:chemistry*; D045542:PQQ Cofactor; D010940:Plant Proteins / Q000737:chemistry*; D015363:Quinolones / Q000737:chemistry*; D013057:Spectrum Analysis
D003067:Coenzymes; D005656:Fungal Proteins; D010940:Plant Proteins; D015363:Quinolones; D045542:PQQ Cofactor; D010088:Oxidoreductases; D005692:Galactose Oxidase; D042845:Laccase; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors