Hyperpolarization of the cell membrane of mouse hepatocytes by metabolizable and nonmetabolizable monosaccharides.
The effect of metabolizable (D-glucose, D-fructose) and nonmetabolizable (2-deoxy-D-glucose, L-glucose) monosaccharides on the membrane potential (Vm) of mouse hepatocytes was investigated employing a superfused liver slice technique. D-Cellobiose was used as an osmotic control. All monosaccharides tested hyperpolarized the liver cell membrane. The short-term effects of D-glucose, D-fructose and 2-deoxy-D-glucose were similar, whereas the effect of L-glucose was less pronounced. The K+ channel blocker quinine reversed the effects of glucose and 2-deoxy-D-glucose on the Vm, suggesting that opening of K+ channels is involved in the hyperpolarizing effect of monosaccharides. The bearing of these findings with regard to hepatic control of food intake is discussed. The findings argue against a role of hepatocytes as glucoreceptors sensing glucose metabolism.
Meyer
A H
AH
Institut für Veterinär-Physiologie, Universität Zürich, Switzerland.
Scharrer
E
E
D000818:Animals; D002462:Cell Membrane / Q000502:physiology*; D046508:Culture Techniques; D005260:Female; D008099:Liver / Q000166:cytology*; D008297:Male; D008564:Membrane Potentials / Q000502:physiology*; D051379:Mice; D009005:Monosaccharides / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000502:physiology
D009005:Monosaccharides; D011956:Receptors, Cell Surface; C027786:glucose receptor