Electrophysiological and behavioral studies on the taste of umami substances in the rat.
Electrophysiological and behavioral experiments were performed to reveal taste properties of "umami" substances such as monosodium glutamate (MSG) and disodium inosine monophosphate (IMP) in rats. To eliminate the taste effects of Na ions contained in these umami substances, we dissolved them in 0.01 mM amiloride, which is known to block sodium responses. In the electrophysiological study, taste responses of the whole chorda tympani nerve were recorded. The magnitude of responses to MSG (or IMP) at concentrations below 0.1 M (or 0.01 M) was less than 10% of that to 0.1 M NaCl. On the other hand, the mixtures of MSG and IMP showed responses 2-7 times larger than the arithmetric sum of the responses to each component of the mixtures. A new sweet taste inhibitor (Gymnema sylvestre extract) strongly suppressed neural responses to mixtures of MSG and IMP as well as sucrose, but only weakly or negligibly to individual solutions of these umami substances. In the behavioral study, the brief exposure two-bottle preference test and conditioned taste aversion paradigm were used. MSG was most preferred at 0.3 M (preference ratio = 57%), IMP, at 0.01 M (61%), and both were less preferred or rejected at higher concentrations. In contrast, mixtures of MSG and IMP were more preferred at a broad concentration range (e.g., 82% for 0.1 M MSG + 0.01 M IMP). Aversive conditioning to umami substances was generalized to sucrose, and vice versa, but not to 0.1 M NaCl, 0.01 M HCl, and 0.1 mM quinine hydrochloride.(ABSTRACT TRUNCATED AT 250 WORDS)
Yamamoto
T
T
Department of Behavioral Physiology, Faculty of Human Sciences, Osaka University, Japan.
Matsuo
R
R
Fujimoto
Y
Y
Fukunaga
I
I
Miyasaka
A
A
Imoto
T
T
D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D002814:Chorda Tympani Nerve / Q000502:physiology*; D003214:Conditioning, Classical / Q000502:physiology*; D004193:Discrimination Learning / Q000502:physiology; D005073:Evoked Potentials, Somatosensory / Q000502:physiology; D005971:Glutamates / Q000502:physiology; D007291:Inosine Monophosphate; D008297:Male; D009433:Neural Inhibition / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D015222:Sodium Channels / Q000502:physiology; D012970:Sodium Glutamate; D013329:Structure-Activity Relationship; D013649:Taste / Q000502:physiology*; D013650:Taste Buds / Q000502:physiology*
D005971:Glutamates; D015222:Sodium Channels; D007291:Inosine Monophosphate; D012970:Sodium Glutamate