Neurokinin A receptors are coupled to calcium mobilization in transfected fibroblasts.
Neurokinin A (NKA) mediated a concentration dependent increase in the intracellular free Ca2+ concentration, [Ca2+]i, in B82 fibroblasts transfected with the neurokinin 2 (NK2) receptor. The EC50 value of this response was 24 nM. A selective NK2 antagonist, MEN 10207, at a concentration of 1 microM completely inhibited the [Ca2+]i rise to 0.1 microM NKA. These results suggest that activation of NK2 receptors expressed in the transfected fibroblasts are functionally coupled to intracellular calcium mobilization.
Henderson
A K
AK
Department of Pharmacology, University of Arizona, College of Medicine, Tucson 85724.
Yamamura
H I
HI
Maggi
C A
CA
Roeske
W R
WR
Smith
T L
TL
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D015288:Neurokinin A / Q000031:analogs & derivatives / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014162:Transfection
D010446:Peptide Fragments; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter; C063615:neurokinin A (4-10), Tyr(5)-Trp(6,8,9)-Arg(10)-; D015288:Neurokinin A; D002118:Calcium