Inhibition of human immunodeficiency virus 1 (HIV-1) and herpes simplex virus 1 (HSV-1) infectivity with a broad range of lectins.
Five lectins with specificity for N- and O-linked oligosaccharides were examined for inhibition of HIV-1 and HSV-1 infectivity in vitro. HIV-1 isolate HTLVIIIB was preincubated with lectin and subsequently inoculated onto MT-4 cells. Lectins specific for N-linked oligosaccharides blocked HIV infection in nanomolar-micromolar concentrations, but no anti-HIV effect was found with the lectin HPA, mainly reacting with O-linked oligosaccharides. HSV-1 infectivity was measured in a plaque reduction assay using Vero cells, and while both N- and O-linked oligosaccharide -specific lectins inhibited HSV-1 infection, the most potent inhibition was found with the lectin HPA. These results indicate that lectins may have a broad antiviral effect on enveloped viruses only limited by types of oligosaccharides present on individual viruses.
Hansen
J E
JE
Department of Infectious Diseases, Hvidovre Hospital, Denmark.
Nielsen
C
C
Vestergaard
B F
BF
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D015488:HIV Antigens / Q000032:analysis / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D037102:Lectins / Q000276:immunology*; D009500:Neutralization Tests; D009844:Oligosaccharides / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology*; D014709:Vero Cells
D015488:HIV Antigens; D037102:Lectins; D009844:Oligosaccharides