The role of taurine in the survival and function of cerebellar cells in cultures of early postnatal cat.
The role of taurine and beta-alanine was analyzed in kitten cerebellar cultures. Since in contrast to mouse, cats (and primates including man) cannot synthesize sufficient taurine to maintain their body pools, we considered the cat an ideal species for the analysis of the role of taurine during early postnatal cerebellar development under controlled conditions. Unexpectedly, we found that the presence of taurine was toxic to neurons but that compounds, considered to be competitors for the beta-amino acid uptake system, support cell survival and cell function in vitro, the opposite of the results found in mice. This could be explained by the finding that only minute amounts of [3H]taurine were taken up by both cat neurons and glial cells under optimal culture conditions but that in the presence of the taurine analogues beta-alanine and guanidinoethane sulfonic acid (GES) significant amounts of taurine were found in all cell types. These differences between mouse cerebellar cells and cat cerebellar cells in vitro suggest that a re-evaluation of the mechanisms that control taurine function in cats and primates is warranted.
Trenkner
E
E
Department of Developmental Biochemistry, Institute for Basic Research in Developmental Disabilities, Staten Island, NY 10314.
Sturman
J A
JA
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002415:Cats; D002452:Cell Count; D002470:Cell Survival; D002531:Cerebellum / Q000166:cytology* / Q000378:metabolism; D005971:Glutamates / Q000493:pharmacokinetics; D018698:Glutamic Acid; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013654:Taurine / Q000493:pharmacokinetics / Q000502:physiology*; D015091:beta-Alanine / Q000493:pharmacokinetics
D005971:Glutamates; D015091:beta-Alanine; D013654:Taurine; D018698:Glutamic Acid