[Proliferation of Reed-Sternberg cells in 5 Hodgkin's cell lines].
Using the monoclonal antibody Ki-67 that detects a cell proliferation-associated human nuclear antigen, incorporations of bromodeoxyuridine (Brud) and 3H-Thymidine, we studied the proliferative capacity of Reed-Sternberg (RS) cells in Hodgkin's cell lines L428, KM-H2, Holden, L540 and L591. The results revealed that in all cell lines, except L591, more than 55% of RS cells were found positive for Ki-67, and over 50% of them had Brud incorporation 5 days after cultivation. The positive score for 3H-Thymidine incorporation was low, and it is assumed that the time taken for 3H-Thymidine in cultivation was short. It shows that at least half of RS cells in these cell lines have proliferation-associated nuclear antigen or can synthesize DNA, and so they are proliferative.
Tang
Y
Y
Institute of Pathology, Klinikum Steglitz, Freien Universitaet Berlin, Federal Republic of Germany.
Schwarting
R
R
Stein
H
H
D000911:Antibodies, Monoclonal / Q000032:analysis; D034961:Antigens, Nuclear; D002455:Cell Division; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006689:Hodgkin Disease / Q000473:pathology*; D006801:Humans; D009687:Nuclear Proteins / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000502:physiology
D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D004273:DNA, Neoplasm; D009687:Nuclear Proteins