title: Identification of rabbit uterine cervical procollagenase activator as rabbit matrix metalloproteinase 3 (stromelysin).
abstract: 1. We previously reported an endogenous activator of procollagenase from the culture medium of rabbit uterine cervical fibroblasts (Ishibashi et al. (1987) Biochem. J. 241, 527-534). 2. Similar activator was also purified and characterized from rabbit synovial fibroblasts (Vater et al. (1983) J. biol. Chem. 258, 9374-9382), but its mode of activation of procollagenase was reported to be different from that of purified activator from uterine cervical fibroblasts. 3. Here we report the comparative studies of the two activators of procollagenase and demonstrate that they are identified as matrix metalloproteinase 3 (stromelysin) by their immunological and functional criteria. The specific role of the activator in procollagenase activation is also described.
authors.affiliation: Department of Biochemistry, Tokyo College of Pharmacy, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Nagase
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000201:enzymology*; D002846:Chromatography, Affinity; D017364:Collagenases; D004792:Enzyme Precursors / Q000378:metabolism*; D005260:Female; D005779:Immunodiffusion; D007700:Kinetics; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D003012:Microbial Collagenase / Q000378:metabolism*; D011247:Pregnancy; D011817:Rabbits
chemical_list: D004792:Enzyme Precursors; D017364:Collagenases; D008666:Metalloendopeptidases; C022440:procollagenase; D019278:Matrix Metalloproteinase 3; D003012:Microbial Collagenase