Specific adhesion of carcinoembryonic antigen-bearing colorectal cancer cells to immobilized carcinoembryonic antigen.
Recent characterization of the genomic structure of carcinoembryonic antigen (CEA) is consistent with that of a cellular adhesion molecule. To examine this function in colorectal cancer, the adherence of cell lines to microtiter wells coated with CEA and well-described adhesive molecules was determined. The CEA-positive cell line LoVo and the CEA-devoid cell line H-Meso-1 did not differ in adherence to the extracellular matrix proteins laminin, collagen and fibronectin, whereas LoVo cells adhered to CEA (10 micrograms/well) in a specific manner (43% bound cells vs. 1.5% bound cells with BSA or alpha-acidglycoprotein controls, P less than 0.01) while H-MESO-1 showed no adhesion to CEA (less than 0.6% bound cells). This adhesion of LoVo cells to CEA was not affected by co-incubation of cells with EDTA, sodium azide, or at 23 degrees C. However, the CEA to CEA adhesive interaction was inhibited by a monoclonal antibody directed against an epitope in the N-terminal domain of the CEA molecule, and decreased by enzymatic removal of CEA from the LoVo cell membrane. The extent of adhesion to immobilized CEA by four CEA-producing cell lines (LoVo, HT29, LS174T and LS174-S), correlated with membrane CEA expression as determined by FACS analysis. The results of these experiments add support to the concept that CEA may function as a specific homotypic cellular adhesion molecule for colorectal cancer cells.
Levin
L V
LV
Division of Hematology/Oncology, University of Massachusetts Medical School, Worcester 01655.
Griffin
T W
TW
D000704:Analysis of Variance; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology*; D002460:Cell Line; D002846:Chromatography, Affinity; D015179:Colorectal Neoplasms / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007797:Laminin / Q000494:pharmacology; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007797:Laminin; D010727:Phosphoric Diester Hydrolases; D043265:Phosphatidylinositol Diacylglycerol-Lyase