Characterization and comparison of avian and murine helper cell lines for production of replication-defective retroviruses for avian transformation.
Several approaches were taken to identify improved helper cell lines for the production of replication-defective avian retroviral vectors for avian transformation. Both QT6 and D17 cells were engineered to become helper cell lines for the production of reticuloendotheliosis virus vectors. The results showed that the majority of lines from the D17, QT6, and D17C3 cells produced titers in the 10(2) to 10(3) cfu/mL range, with one QT6 line producing 10(5) cfu/mL. This high producer line was relatively free of helper virus when restricted to low passage. An amphotropic murine cell line produced a 6- to 10-fold higher amount of virus and had a comparable higher titer on chicken cells, suggesting possible application to avian transformation.
Highkin
M K
MK
Monsanto Company, St. Louis, Missouri 63198.
Krivi
G G
GG
Hippenmeyer
P J
PJ
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D004279:DNA, Viral / Q000032:analysis; D003673:Defective Viruses / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D005347:Fibroblasts / Q000382:microbiology; D005822:Genetic Vectors; D006378:Helper Viruses / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D051379:Mice; D010957:Plasmids; D011784:Quail; D012367:RNA, Viral / Q000032:analysis; D001356:Reticuloendotheliosis virus / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D014162:Transfection; D014170:Transformation, Genetic; D014771:Virion / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D014779:Virus Replication
D004279:DNA, Viral; D012367:RNA, Viral