title: Detection of human polyomavirus DNA in Papanicolaou stained smears of urinary sediment by in situ hybridization.
abstract: Papanicolaou stained smears of urinary sediment containing inclusion bearing urothelial cells suggestive of human polyomavirus infection were destained and reprocessed for in situ hybridization using a biotinylated probe for human polyomavirus DNA. Seven slides were processed in this way. A hybridization signal for viral DNA was noted in each case, even in smears that had previously been stored for 11 years. This simple and rapid non-radioactive detection system is a valuable supplement to routine urinary cytology for the definitive diagnosis of this virus infection.
authors.affiliation: Department of Pathology, National Cardiovascular Center, Osaka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Iwa
authors.forename: T K
authors.initials: TK
authors.lastname: Kobayashi
authors.forename: C
authors.initials: C
authors.lastname: Yutani
mesh_terms: D004279:DNA, Viral / Q000652:urine*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000652:urine
chemical_list: D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic