title: IgM responses in chicken serum to live and inactivated infectious bronchitis virus vaccines.
abstract: Intramuscular (i.m.) administration of infectious bronchitis virus (IBV) oil-emulsion vaccine (OEV) to IBV-primed or unprimed chickens resulted in the production of zero or minimal concentrations of IBV-specific IgM in the serum, as measured by enzyme-linked immunosorbent assay of gel chromatography fractions. Live-attenuated infectious bronchitis (IB) vaccine given i.m. or by eyedrop stimulated the production of IBV-specific IgM in similar amounts following inoculation by both routes. These levels were comparable to those found in earlier studies following intranasal inoculation with a virulent strain of IBV and confirm that the detection of IBV-specific IgM is a valuable aid to the diagnosis of recent infection. As expected, administration of live-attenuated IB vaccines i.m. or by eyedrop protected the respiratory tract against challenge with virulent virus 24 days later; however, OEV given i.m. did not.
authors.affiliation: Instituto de Pesquisas Veterinarias Desiderio Finamor, Porto Alegre, Brazil.
authors.forename: N R
authors.initials: NR
authors.lastname: Martins
authors.forename: A P
authors.initials: AP
authors.lastname: Mockett
authors.forename: A D
authors.initials: AD
authors.lastname: Barrett
authors.forename: J K
authors.initials: JK
authors.lastname: Cook
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D002645:Chickens / Q000276:immunology*; D002850:Chromatography, Gel; D002923:Cilia / Q000502:physiology; D003333:Coronaviridae Infections / Q000517:prevention & control / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007075:Immunoglobulin M / Q000096:biosynthesis* / Q000097:blood; D001351:Infectious bronchitis virus / Q000276:immunology*; D007273:Injections, Intramuscular / Q000662:veterinary; D009500:Neutralization Tests; D009883:Ophthalmic Solutions; D011201:Poultry Diseases / Q000517:prevention & control; D013047:Specific Pathogen-Free Organisms; D014132:Trachea / Q000502:physiology; D014611:Vaccination / Q000662:veterinary; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009883:Ophthalmic Solutions; D015164:Vaccines, Inactivated; D014765:Viral Vaccines