Detection of HHV-6 by the polymerase chain reaction.
The polymerase chain reaction (PCR) was used to detect human herpes virus 6 (HHV-6) sequences in tissue culture. A pair of primers was synthesized and used to amplify a conserved region of the genome. Amplified products were detected either by visualization of UV illuminated ethidium bromide stained gel or, by hybridization with a specific radiolabeled oligonucleotide. As little as 5 fg of HHV-6 could be detected in infected cells, making this assay suitable for diagnostic purposes.
Collandre
H
H
Institut Pasteur Unité d'Oncologie Virale, Paris, France.
Aubin
J T
JT
Agut
H
H
Béchet
J M
JM
Montagnier
L
L
D001483:Base Sequence; D046508:Culture Techniques; D004279:DNA, Viral / Q000032:analysis; D004996:Ethidium; D006566:Herpesviridae Infections / Q000175:diagnosis; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
D004279:DNA, Viral; D004996:Ethidium