[Assessment of 99mTc-DMSA renography and uptake compared with creatinine clearance in rats with drug-induced nephrotoxicity--I. Gentamicin-induced nephrotoxicity].
For evaluation of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) renal uptake as an absolute renal function, 99mTc-DMSA uptake was compared with endogenous creatinine clearance (Ccr) in gentamicin-induced nephrotoxicity. Gentamicin (40 mg/kg/day) was given subcutaneously to male Wistar rats for periods of 3, 6, 9 and 12 days. On the next day, the renography was performed 2 hours following intravenous injection of 99mTc-DMSA and Ccr was measured. On the 7th day, 99mTc-DMSA uptake was significantly lower in the treated rats than that in control (32.27 +/- 0.92 vs 39.84 +/- 2.24%; p less than 0.01), but Ccr did not change (0.37 +/- 0.02 vs 0.34 +/- 0.05 ml/min/100 g; NS). On the 1st, 2nd, 4th and 8th day after single subcutaneous injection of 80 mg of gentamicin, 99mTc-DMSA uptake was measured and the histological examination was done. On the 4th day, 99mTc-DMSA uptake was significantly lower than that on the 1st day (32.32 +/- 3.00 vs 38.91 +/- 1.95%; p less than 0.01) and microscopic examinations revealed that eosinophilic granular degenerations were evident in the renal cortex. The present study suggests that 99mTc-DMSA uptake reduces earlier than Ccr in gentamicin-induced nephrotoxicity and 99mTc-DMSA uptake is a reliable indicator in the evaluation of a renal function in drug-induced nephrotoxicity.
Yamada
M
M
Department of Radiology, Ehime University School of Medicine.
D000818:Animals; D003404:Creatinine / Q000097:blood / Q000652:urine*; D005839:Gentamicins; D007668:Kidney / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000503:physiopathology; D007677:Kidney Function Tests; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics; D011866:Radioisotope Renography; D051381:Rats; D011919:Rats, Inbred Strains; D004113:Succimer / Q000493:pharmacokinetics; D019783:Technetium Tc 99m Dimercaptosuccinic Acid
D005839:Gentamicins; D015609:Organotechnetium Compounds; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D003404:Creatinine; D004113:Succimer