[Effects of cyclosporine A on insulin content and insulin release in neonatal rat islet cell monolayer cultures].
We investigated the effects of cyclosporine A (CsA) on insulin content and insulin release in neonatal rat islet cell monolayer cultures. Insulin content in 5.5-day-old monolayers was not affected after 12-hour incubation in the medium containing 16.7 mmol/L glucose plus 0.1 or 100 micrograms/ml CsA and 5.6 mmol/L glucose plus 10 micrograms/ml CsA. Insulin release in 7.5-day-old monolayers was inhibited after 12-hour incubation in the medium containing 5.6 or 16.7 mmol/L glucose plus 0.01-100 micrograms/ml CsA in a dose-responsive manner. At 16.7 mmol/L glucose, CsA less than or equal to 0.1 microgram/ml did not affect insulin release, while CsA greater than or equal to 1.0 microgram/ml significantly inhibited insulin release and the inhibitory effect did not return to normal after a 16-hour incubation in CsA-free medium. At 5.6 mmol/L glucose, CsA 1.0 microgram/ml did not affect insulin release. CsA 100 micrograms/ml inhibited insulin release less than it did at 16.7 mmol/L glucose. These experimental data demonstrated that high doses of CsA have inhibitory effects on insulin release in islet monolayer cultures.
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D000818:Animals; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D016572:Cyclosporine