Glutathione-induced enhancement of neutrophil locomotion.
Both reduced glutathione (GSH) and and its oxidized form, glutathione disulfide (GSSG), enhance neutrophil locomotion. The enhancement is mainly due to a chemokinetic effect, and partly due to a chemotactic effect. A number of other SH-group containing compounds were not effective in enhancing neutrophil migration. While random locomotion is not inhibited by the slowly-penetrating sulfhydryl agent 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), the enhancement of migration due to GSH is completely inhibited. Pretreatment of neutrophils with pertussis toxin completely inhibited the GSH-induced stimulation of locomotion, suggesting that stimulation of migration by glutathione was mediated by a pertussis toxin sensitive G-protein. Chemotaxis towards GSH is inhibited by the same agents that inhibit fMet-Leu-Phe induced chemotaxis, except that colchicine was a more effective inhibitor of GSH-induced chemotaxis than of fMet-Leu-Phe directed chemotaxis. GSH enhances the intracellular concentration of cGMP, which might indicate that the effect on neutrophil locomotion is mediated by an effect on cGMP.
Elferink
J G
JG
Department of Medical Biochemistry, University of Leiden, The Netherlands.
de Koster
B M
BM
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D006152:Cyclic GMP / Q000378:metabolism; D005978:Glutathione / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D010529:Peritoneal Cavity / Q000166:cytology; D011817:Rabbits
D009240:N-Formylmethionine Leucyl-Phenylalanine; D005978:Glutathione; D006152:Cyclic GMP