title: Polyclonal and monoclonal antibodies against human tissue inhibitor of metalloproteinases (TIMP) and the design of an enzyme-linked immunosorbent assay to measure TIMP.
abstract: Both polyclonal and monoclonal antibodies have been raised against purified human fibroblast tissue inhibitor of metalloproteinases (TIMP) and characterised. Combinations of antibodies were tested for their suitability in a double antibody sandwich ELISA to measure TIMP. Two combinations were applicable to the immunoassay: (i) a monoclonal capture antibody with a polyclonal detecting antibody; (ii) two monoclonal antibodies. The assay range was (i) 2-50 ng/ml and (ii) 5-50 ng/ml of human TIMP. The levels of TIMP in several human body fluids were measured using assay (ii), and values in ng/ml with standard deviations (n-1) obtained as follows: serum, 299 +/- 62; plasma, 109 +/- 35; amniotic fluid, 724 +/- 62; cerebrospinal fluid, 144 +/- 156; saliva, 209 +/- 103.
authors.affiliation: Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge, UK.
authors.forename: I M
authors.initials: IM
authors.lastname: Clark
authors.forename: L K
authors.initials: LK
authors.lastname: Powell
authors.forename: J K
authors.initials: JK
authors.lastname: Wright
authors.forename: T E
authors.initials: TE
authors.lastname: Cawston
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000917:Antibody Formation; D015153:Blotting, Western; D001826:Body Fluids / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D003012:Microbial Collagenase / Q000032:analysis / Q000037:antagonists & inhibitors*; D011817:Rabbits; D019714:Tissue Inhibitor of Metalloproteinases
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D019714:Tissue Inhibitor of Metalloproteinases; D003012:Microbial Collagenase