The orphan receptor cDNA RDC4 encodes a 5-HT1D serotonin receptor.
The cDNA of RDC4, a putative receptor of the G protein-coupled receptor family, has been cloned by PCR methodology. The primary structure of this receptor showed homology with the serotonin 5-HT1A receptor. In this work, RDC4 mRNA has been injected in Y1 adrenal cells and Xenopus oocytes and RDC4 cDNA has been transfected transiently in cos-7 cells. In all these systems serotonin elicited a rise in cyclic AMP levels. Binding studies on membranes of the transfected cos-7 cells using [3H]-LSD showed a pattern of drug affinities consistent with the known properties of a 5-HT1D receptor. RDC4 therefore codes for a 5-HT1D receptor which in the studied systems is positively coupled to adenylate cyclase.
Maenhaut
C
C
Institut de Recherche Interdisciplinaire, Faculté de Médecine, Université Libre de Bruxelles, Belgium.
Van Sande
J
J
Massart
C
C
Dinsart
C
C
Libert
F
F
Monferini
E
E
Giraldo
E
E
Ladinsky
H
H
Vassart
G
G
Dumont
J E
JE
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000302:Adrenal Cortex; D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D004247:DNA / Q000235:genetics; D019204:GTP-Binding Proteins / Q000378:metabolism*; D007700:Kinetics; D008237:Lysergic Acid / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D011985:Receptors, Serotonin / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013764:Tetrahydronaphthalenes / Q000494:pharmacology; D014162:Transfection
D012333:RNA, Messenger; D011985:Receptors, Serotonin; D011994:Recombinant Proteins; D013764:Tetrahydronaphthalenes; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D004247:DNA; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D008237:Lysergic Acid