Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum.
The postnatal development of calcium-mobilizing systems was studied by both microfluorometric imaging analysis of Ca2+ on living rat cerebellar slices and immunohistochemical labeling of phosphatidylinositol 4,5-bisphosphate (PIP2) and inositol 1,4,5-trisphosphate binding protein (IP3BP) in fixed rat cerebellum. Stimulation with quisqualate (QA) or N-methyl-D-aspartate (NMDA) enhanced the Ca2+ level only diffusely on postnatal day (PND) 3, but more discretely on PNDs 7 and 15. On PND 21, QA-induced responses were localized in the molecular layer especially, but not in the granular layer. By contrast, NMDA mobilized Ca2+ prominently in the granular layer, but only weakly in the molecular layer. Localized expression of PIP2 in the molecular layer paralleled QA-induced Ca2+ mobilization, but IP3BP was expressed more diffusely. The present study offers the first direct evidence that PIP2, but not IP3BP, is essential for QA-induced Ca2+ mobilization in the cerebellar cortex.
Ito
E
E
Advanced Research Center for Human Sciences, Waseda University, Tokorozawa, Japan.
Miyazawa
A
A
Takagi
H
H
Yoshioka
T
T
Horikoshi
T
T
Yanagisawa
K
K
Nakamura
T
T
Kudo
Y
Y
Umeda
M
M
Inoue
K
K
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels; D002525:Cerebellar Cortex / Q000254:growth & development / Q000378:metabolism; D002531:Cerebellum / Q000254:growth & development* / Q000378:metabolism; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D053496:Inositol 1,4,5-Trisphosphate Receptors; D016202:N-Methylaspartate / Q000494:pharmacology; D010770:Phosphotransferases / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D011485:Protein Binding; D016318:Quisqualic Acid / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000378:metabolism
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D016202:N-Methylaspartate; D015544:Inositol 1,4,5-Trisphosphate; D016318:Quisqualic Acid; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C068961:phosphatidylinositol 4,5-biphosphate kinase; D002118:Calcium