title: Pseudomonas aeruginosa cholinesterase and phosphorylcholine phosphatase: two enzymes contributing to corneal infection.
abstract: Choline, acetylcholine and betaine used as the sole carbon, nitrogen or carbon and nitrogen source increase cholinesterase activity in addition to phosphorylcholine phosphatase and phospholipase C activities in Pseudomonas aeruginosa. The cholinesterase activity catalyses the hydrolysis of acetylthiocholine (Km approx. 0.13 mM) and propionylthiocholine (Km approx. 0.26 mM), but not butyrylthiocholine, which is a pure competitive inhibitor (Ki 0.05 mM). Increasing choline concentrations in the assay mixture decreased the affinity of cholinesterase for acetylthiocholine, but in all cases prevented inhibition raised by high substrate concentrations. Considering the properties of these enzymes, and the fact that in the corneal epithelium there exists a high acetylcholine concentration and that Pseudomonas aeruginosa produces corneal infection, it is proposed that these enzymes acting coordinately might contribute to the breakdown of the corneal epithelial membrane.
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
authors.forename: C E
authors.initials: CE
authors.lastname: Domenech
authors.forename: M N
authors.initials: MN
authors.lastname: Garrido
authors.forename: T A
authors.initials: TA
authors.lastname: Lisa
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000122:Acetylthiocholine / Q000378:metabolism; D001622:Betaine / Q000378:metabolism; D002092:Butyrylthiocholine / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D002802:Cholinesterases / Q000378:metabolism*; D003316:Corneal Diseases / Q000382:microbiology; D015818:Eye Infections, Bacterial / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000378:metabolism / Q000472:pathogenicity; D013860:Thiocholine / Q000031:analogs & derivatives / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: C009063:propionylthiocholine; D001622:Betaine; D000122:Acetylthiocholine; D002092:Butyrylthiocholine; D013860:Thiocholine; D002802:Cholinesterases; C054085:phosphocholine phosphatase; D010744:Phosphoric Monoester Hydrolases; D010738:Type C Phospholipases; D002794:Choline; D000109:Acetylcholine