Reduction by interferon-alpha of levels of c-myc protein and DNA synthesis in a human hepatoma cell line mediated by inhibition of putrescine synthesis.
Interferon-alpha inhibited increases in ornithine decarboxylase, intracellular putrescine, and DNA synthesis as human hepatoma cells were stimulated to grow. Interferon-alpha inhibited the increase in the c-myc protein level, but not its mRNA level. Added putrescine abrogated the effects on c-myc protein and DNA synthesis. Interferon-alpha seemed to inhibit the increase in the c-myc protein level post-transcriptionally by reducing the putrescine level, inhibiting DNA synthesis.
Takeda
T
T
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
Nishiguchi
S
S
Kuroki
T
T
Kobayashi
K
K
Hasuma
T
T
Matsui-Yuasa
I
I
Otani
S
S
D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007700:Kinetics; D008113:Liver Neoplasms; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D011700:Putrescine / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D007370:Interferon Type I; D011073:Polyamines; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D009955:Ornithine Decarboxylase; D011700:Putrescine