Development and evaluation of an enzyme-linked immunosorbent assay for detection, typing, and subtyping of vesicular stomatitis virus.
An indirect sandwich enzyme-linked immunosorbent assay (ELISA) has been used for vesicular stomatitis virus (VSV) typing using sets of monovalent and polyvalent rabbit/guinea pig antisera for identification of VSV types New Jersey (VNJ) and Indiana (VIND). The VIND polyvalent antiserum (VIND-P) detects any strain of the 3 subtypes of the VIND type (VIND-1, VIND-2, and VIND-3) with the same strong reactivity. It is also possible to subtype the VIND strains using VIND-P rabbit antiserum as capture antibody and monovalent VIND-1, VIND-2, or VIND-3 guinea pig antisera as detector. The ELISA proposed has about 10 times more sensitivity and provides 10% more positive results than does the complement fixation 50% (CF50) test when epithelial samples are tested.
Alonso
A
A
Pan American Foot-and-Mouth Disease Center, Rio de Janeiro, Brazil.
Martins
M A
MA
Gomes
M da P
Mda P
Allende
R
R
Söndahl
M S
MS
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000382:microbiology; D003168:Complement Fixation Tests; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D011237:Predictive Value of Tests; D011817:Rabbits; D012703:Serotyping; D013280:Stomatitis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology; D014721:Vesicular stomatitis Indiana virus / Q000145:classification / Q000302:isolation & purification*; D018116:Vesiculovirus; D014777:Virus Diseases / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*
D000956:Antigens, Viral; D007106:Immune Sera