Nucleotide sequence of xylE from the TOL pDK1 plasmid and structural comparison with isofunctional catechol-2,3-dioxygenase genes from TOL, pWW0 and NAH7.
Detailed restriction and nucleotide sequence analysis of the Pseudomonas putida TOL plasmid pDK1 xylE gene revealed significant homology with isofunctional xylE (81.5%) and nahH (78.0%) genes from the TOL pWW0 and NAH7 plasmids. The highest degrees of nucleotide and apparent amino acid conservation (82.2 and 86.4%, respectively) among all three genes were found to exist within a region comprising 264 nucleotides encoding the C terminus. A comparison of localized regions revealed significantly greater homology between xylEpWW0 and xylEpDK1 within the C-terminal region, whereas xylEpWW0 and nahH showed greater similarity at the N terminus. The possibility that xylEpWW0 may represent a genetic hybrid of xylEpDK1 and nahH is discussed.
Benjamin
R C
RC
Department of Biological Sciences, University of North Texas, Denton 76203.
Voss
J A
JA
Kunz
D A
DA
D000595:Amino Acid Sequence; D001483:Base Sequence; D050557:Catechol 2,3-Dioxygenase; D049308:Dioxygenases; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011549:Pseudomonas / Q000235:genetics*; D015183:Restriction Mapping
D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase