title: MutM, a protein that prevents G.C----T.A transversions, is formamidopyrimidine-DNA glycosylase.
abstract: We have cloned chromosomal DNA bordering an insert that inactivates mutM. Sequencing of this clone has revealed that the insertion element is located between the promoter and structural gene for formamidopyrimidine-DNA glycosylase (Fapy-DNA glycosylase). An overproducing clone of Fapy-DNA glycosylase complements the original mutM strain that had been isolated after EMS mutagenesis. Thus, we conclude that MutM is actually Fapy-DNA glycosylase. mutM has previously been characterized as a mutator strain that leads specifically to G.C----T.A transversions. This in vivo characterization correlates well with the mutagenic potential of one of the lesions Fapy-DNA glycosylase removes, 8-oxo-7,8-dihydro-2'-deoxyguanine (8-OxodG).
authors.affiliation: Molecular Biology Institute, University of California, Los Angeles 90024.
authors.forename: M L
authors.initials: ML
authors.lastname: Michaels
authors.forename: L
authors.initials: L
authors.lastname: Pham
authors.forename: C
authors.initials: C
authors.lastname: Cruz
authors.forename: J H
authors.initials: JH
authors.lastname: Miller
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004260:DNA Repair / Q000235:genetics* / Q000502:physiology; D004251:DNA Transposable Elements / Q000235:genetics*; D043382:DNA-Formamidopyrimidine Glycosylase; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000633:toxicity; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics; D009699:N-Glycosyl Hydrolases / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase; C468610:DNA-formamidopyrimidine glycosylase, E coli; D003849:Deoxyguanosine