title: Formation of lentivirus particles by mammalian cells infected with recombinant fowlpox virus.
abstract: Recombinant fowlpox viruses (FPV) containing the env or gag-pol genes of simian immunodeficiency virus from macaques (SIVmac) were constructed. The env, gag, and pol-encoded polypeptides were efficiently expressed and processed in avian cells productively infected with FPV as well as in mammalian cells, in which FPV infection is abortive. In addition, the recombinant FPV expressing the gag-pol genes directed the formation of defective, lentivirus-like particles which were released into the culture medium of infected cells. Coinfection of cells with the env and gag-pol recombinant viruses resulted in the generation of particles containing SIVmac envelope glycoprotein. The applications of this system to vaccine development are discussed.
authors.affiliation: Therion Biologics Corporation, Cambridge, MA 02142.
authors.forename: S
authors.initials: S
authors.lastname: Jenkins
authors.forename: L
authors.initials: L
authors.lastname: Gritz
authors.forename: C H
authors.initials: CH
authors.lastname: Fedor
authors.forename: E M
authors.initials: EM
authors.lastname: O'Neill
authors.forename: L K
authors.initials: LK
authors.lastname: Cohen
authors.forename: D L
authors.initials: DL
authors.lastname: Panicali
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D004274:DNA, Recombinant; D005587:Fowlpox virus / Q000235:genetics*; D015752:Genes, env; D015750:Genes, gag; D015751:Genes, pol; D005822:Genetic Vectors; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012191:Retroviridae Proteins / Q000096:biosynthesis*; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure
chemical_list: D004274:DNA, Recombinant; D012191:Retroviridae Proteins