Formation of subviral particles by in vitro translation of subgenomic poliovirus RNAs.
Rabbit reticulocyte lysates were programmed with either RNA extracted from purified poliovirus or a mixture of mRNAs encoding the capsid precursor, P1, and proteinase 3CD. In both cases, 14S subunits were formed at 30 degrees C and empty capsids at 37 degrees C. Both the 14S subunits and empty capsids had the expected polypeptide composition and neutralization epitopes. It is concluded that the proteinase 3CD gene is the only viral genetic information needed for the correct processing of P1 and the formation of 14S subunits, and their assembly into antigenically correct empty capsids.
Jore
J P
JP
Medical Biological Laboratory TNO, Rijswijk, The Netherlands.
Veldhuisen
G
G
Pouwels
P H
PH
Boeyé
A
A
Vrijsen
R
R
Rombaut
B
B
D000086042:3C Viral Proteases; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002213:Capsid / Q000235:genetics / Q000276:immunology; D002474:Cell-Free System; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000276:immunology; D017955:Poliovirus / Q000235:genetics* / Q000276:immunology; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics / Q000276:immunology; D012367:RNA, Viral / Q000378:metabolism; D011817:Rabbits; D014764:Viral Proteins
D000956:Antigens, Viral; D011498:Protein Precursors; D012367:RNA, Viral; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases