Expression cloning and characterization of the TGF-beta type III receptor.
The rat TGF-beta type III receptor cDNA has been cloned by overexpression in COS cells. The encoded receptor is an 853 amino acid protein with a large N-terminal extracellular domain containing at least one site for glycosaminoglycan addition, a single hydrophobic transmembrane domain, and a 41 amino acid cytoplasmic tail with no obvious signaling motif. Introduction of the cDNA into COS cells and L6 myoblasts induces expression of a heterogenously glycosylated 280-330 kd protein characteristic of the type III receptor that binds TGF-beta 1 specifically. In L6 myoblasts lacking the endogenous type III receptor, expression of the recombinant receptor leads to an increase in the amount of ligand bound and cross-linked to surface type II TGF-beta receptors. This indicates that the type III receptor may regulate the ligand-binding ability or surface expression of the type II receptor.
Wang
X F
XF
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
Lin
H Y
HY
Ng-Eaton
E
E
Downward
J
J
Lodish
H F
HF
Weinberg
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011509:Proteoglycans / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000145:classification / Q000235:genetics* / Q000276:immunology; D018125:Receptors, Transforming Growth Factor beta; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; C071736:betaglycan; D004247:DNA