title: Phosphatidyltransferase activity in Bacillus megaterium.
abstract: Phosphatidyl transfer between phosphatidylethanolamine, phosphatidylglycerol or phosphatidylserine as donors and primary hydroxyl acceptors including ethanolamine, glycerol, serine and Triton X-100 has been shown to be catalysed by membrane particles derived from Bacillus megaterium strains ATCC 13632 and ATCC 14581. The rate of cardiolipin synthesis from phosphatidylglycerol in the presence of ethanolamine was an order of magnitude greater than that of phosphatidylethanolamine formation. Cardiolipin synthesis from phosphatidylethanolamine in the presence of glycerol was also observed, and was 1.5-fold greater than the formation of phosphatidylglycerol. Similar heat lability, effects of pH and of Triton X-100 for phosphatidyl transfer and cardiolipin synthesis indicate that both reactions were catalysed by cardiolipin synthase.
authors.affiliation: Department of Microbiology, University of Pennsylvania, School of Medicine, Philadelphia 19104-6076.
authors.forename: H
authors.initials: H
authors.lastname: Morii
authors.forename: H
authors.initials: H
authors.lastname: Goldfine
mesh_terms: D001410:Bacillus megaterium / Q000201:enzymology*; D002308:Cardiolipins / Q000096:biosynthesis*; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008565:Membrane Proteins; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010715:Phosphatidylglycerols / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013696:Temperature; D017855:Transferases (Other Substituted Phosphate Groups)
chemical_list: D002308:Cardiolipins; D004983:Ethanolamines; D008565:Membrane Proteins; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010718:Phosphatidylserines; D012694:Serine; D019856:Ethanolamine; D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C100091:cardiolipin synthetase; D005990:Glycerol