title: Characterization of sulfonamide resistance determinants and relatedness of Bordetella avium R plasmids.
abstract: Five plasmids, varying in size from 16 to 51.5 kb, were isolated from virulent strains of Bordetella avium and compared by restriction endonuclease digestion and DNA-DNA hybridization. These plasmids confer resistance to streptomycin and sulfonamides, and three of the five also confer resistance to tetracycline, but they are not closely related. Four of the plasmids, pRL100, p4093, pCW, and pWAM, carried determinants related to the heat-labile type I plasmid-mediated dihydropteroate synthase of the plasmid R388, while one plasmid, p4168, carried a determinant related to the heat-stable type II dihydropteroate synthase of pGS05.
authors.affiliation: Department of Microbiology, North Carolina State University, Raleigh 27695-7615.
authors.forename: D L
authors.initials: DL
authors.lastname: Cutter
authors.forename: G H
authors.initials: GH
authors.lastname: Luginbuhl
mesh_terms: D001884:Bordetella / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004094:Dihydropteroate Synthase / Q000235:genetics; D004352:Drug Resistance, Microbial; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011815:R Factors; D015183:Restriction Mapping; D013307:Streptomycin / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology*; D013753:Tetracycline Resistance
chemical_list: D013449:Sulfonamides; D004094:Dihydropteroate Synthase; D004262:DNA Restriction Enzymes; D013307:Streptomycin