Identification of an ectopic developmental antigen that appears in malignant ascitic fluid.
A tumor-associated antigen that shares antigenicity with a pregnancy-associated protein has been detected in ascitic fluid of patients with advanced ovarian cancer. The protein, prepared from malignant ascitic fluid by the combination of poly(ethylene glycol) 4000 fractionation, DEAE-Sepharose column chromatography and preparative polyacrylamide gel electrophoresis (PAGE), was subsequently separated on sodium dodecyl sulfate-PAGE, as a single band corresponding to an approximate molecular mass of 91 kDa. Immunological analysis showed that this protein was a circulating species of an ectopic developmental antigen that markedly increases in biological fluids in tumor-bearing statuses.
Nakai
M
M
Department of Obstetrics and Gynecology, Kitasato University School of Medicine, Kanagawa, Japan.
Ishikawa
M
M
Kawauchi
H
H
D000951:Antigens, Neoplasm / Q000032:analysis* / Q000302:isolation & purification; D001202:Ascitic Fluid / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000032:analysis; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D009373:Neoplasms, Germ Cell and Embryonal / Q000276:immunology*; D010051:Ovarian Neoplasms / Q000276:immunology*; D011257:Pregnancy Proteins / Q000032:analysis* / Q000302:isolation & purification; D014594:Uterine Neoplasms / Q000276:immunology*
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D011257:Pregnancy Proteins