title: Comparison of binding characteristics of endothelin receptors on subpopulations of astrocytes.
abstract: Astrocytes in primary culture originating from different brain areas of the mouse embryo (striatum, cerebral cortex and mesencephalon) were compared for their [125I]-Endothelin-1 (ET-1) binding characteristics, in terms of affinity, binding capacity and specificity. Our results indicate that astrocytes from mesencephalon express about twice as many receptors as astrocytes from striatum or cortex (149,000 +/- 9700 vs 63,700 +/- 5600 and 81,900 +/- 5300, respectively), with similar affinities. Specificity patterns for the various peptides of the endothelins/sarafotoxins family (ET-1, -2, -3; SRTXa, b, c) are comparable in the three subpopulations of astrocytes: ET-1, -2 and SRTXb exhibit higher affinities than SRTXa and SRTXc. In addition, ET-3 and SRTXc seem to discriminate between different subsets of [125I]-ET-1 binding sites in the three subpopulations.
authors.affiliation: Laboratoire d'Immuno Pharmacologie Moléculaire, C.N.R.S., Paris, France.
authors.forename: P O
authors.initials: PO
authors.lastname: Couraud
authors.forename: O
authors.initials: O
authors.lastname: Durieu-Trautmann
authors.forename: E
authors.initials: E
authors.lastname: Mahé
authors.forename: P
authors.initials: P
authors.lastname: Marin
authors.forename: D
authors.initials: D
authors.lastname: Le Nguyen
authors.forename: A D
authors.initials: AD
authors.lastname: Strosberg
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001667:Binding, Competitive; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000196:embryology / Q000378:metabolism; D003342:Corpus Striatum / Q000196:embryology / Q000378:metabolism; D016232:Endothelins / Q000378:metabolism*; D007700:Kinetics; D008636:Mesencephalon / Q000196:embryology / Q000378:metabolism; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017466:Receptors, Endothelin
chemical_list: D016232:Endothelins; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin