title: Photooxidation of high-potential (c559, c556) and low-potential (c552) hemes in the cytochrome subunit of Rhodopseudomonas viridis reaction center. Characterization by FTIR spectroscopy.
abstract: The photooxidation of c559, c556 and c552 hemes in Rhodopseudomonas viridis cytochrome has been characterized by light-induced FTIR difference spectroscopy. Apart from the common features at 1659 cm-1 and 1561/1551 cm-1 which could arise from one (or possibly two) peptide bond(s), no evidence for major structural rearrangement of the polypeptide backbone was observed. A significant difference with respect to redox-induced FTIR spectra of cytochrome c is the absence of the Tyr marker at 1514/1518 cm-1 in Rps. viridis cytochrome, indicating that the localized shift of a Tyr side chain observed between ferro- and ferri-cytochrome c does not occur in Rps. viridis cytochrome.
authors.affiliation: DBCM/SBE, CEN Saclay, Gif-sur-Yvette, France.
authors.forename: E
authors.initials: E
authors.lastname: Nabedryk
authors.forename: C
authors.initials: C
authors.lastname: Berthomieu
authors.forename: A
authors.initials: A
authors.lastname: Verméglio
authors.forename: J
authors.initials: J
authors.lastname: Breton
mesh_terms: D003574:Cytochrome c Group / Q000737:chemistry*; D005583:Fourier Analysis; D006418:Heme / Q000737:chemistry*; D010084:Oxidation-Reduction; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D012241:Rhodopseudomonas / Q000201:enzymology / Q000378:metabolism*; D013055:Spectrophotometry, Infrared
chemical_list: D003574:Cytochrome c Group; D045322:Photosynthetic Reaction Center Complex Proteins; C083951:cytochrome c553; C020267:cytochrome c556; D006418:Heme; C022302:cytochrome C-552