Mutations responsible for adaptation of hepatitis A virus to efficient growth in cell culture.
Chimeric genomes of hepatitis A virus strain HM-175 were constructed from cDNA clones of the wild-type virus and its cell culture-adapted variant. RNA transcribed in vitro from each construct was assayed for infectivity by transfection of cultured cells. RNA transcribed from the wild-type cDNA clone was minimally infectious and produced virus that grew inefficiently in vitro, whereas that transcribed from certain chimeric genomes consistently produced virus that grew efficiently in cultured cells. Mutations in the P2 region were found to be necessary for efficient virus growth in vitro, while mutations in the 5' noncoding region imparted a conditional enhancement of growth in vitro.
Emerson
S U
SU
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
McRill
C
C
Rosenblum
B
B
Feinstone
S
S
Purcell
R H
RH
D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004252:DNA Mutational Analysis; D006507:Hepatovirus / Q000235:genetics / Q000254:growth & development*; D066298:In Vitro Techniques; D008253:Macaca mulatta; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics; D013045:Species Specificity; D014162:Transfection; D014779:Virus Replication
D012367:RNA, Viral; D004247:DNA