[Arachidonic acid and its acyclic derivatives regulate the short-term plasticity of the cholinoreceptors of the neurons in the edible snail].
On identified Helix neurones RPa3 and LPa3 using the method of double-electrode clamp technique on the membrane the influence was shown of eicosanoids on the dynamics of inward current extinction caused by the repeated ionophoretic applications of acetylcholine to soma. Extracellular influence of arachidonic acid (50-100 microM) increased the extinction. Phospholipase A2 inhibitor quinacrine hydrochloride (100-600 microM) decreasing the content of arachidonic acid in the cell acted differently. Inhibitor of lipoxygenase oxidation of arachidonic acid (nordihydraquiaretic acid) (3-10 microM) weakened the extinction. Blockader of cyclooxygenase oxidation of arachidonic acid--indomethacin (10-50 microM) did not influence the extinction. All the studied composition decreased the amplitude of input current caused by acetylcholine. The obtained results allowed to suppose that arachidonic acid and its acyclic metabolites formed as a result of lipoxygenase oxidation regulated short-term plasticity of snail neurones cholinoreceptors. Cyclic eicosanoids formed at cyclooxygenase oxidation of arachidonic acid had no regulating influence on cholinoreceptors plasticity.
Pivovarov
A S
AS
Drozdova
E I
EI
Kotliar
B I
BI
D000109:Acetylcholine / Q000008:administration & dosage; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D006372:Helix, Snails; D066298:In Vitro Techniques; D007478:Iontophoresis; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008839:Microelectrodes; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
D001095:Arachidonic Acids; D011950:Receptors, Cholinergic; D010741:Phospholipases A; D054467:Phospholipases A2; D000109:Acetylcholine
Arakhidonovaia kislota i ee atsiklicheskie proizvodnye reguliruiu t kratkovremennuiu plastichnost' kholinoretseptorov neĭronov vinogradnoĭ ulitki.