No retroviremia or pathology in long-term follow-up of monkeys exposed to a murine amphotropic retrovirus.
Four monkeys were exposed to a retroviral vector and replication-competent murine amphotropic retrovirus in a bone marrow transplantation/gene transfer protocol (Kantoff et al., 1987). We have studied these animals 2 and 3 years post-transplantation and did not detect replicating virus in serum, peripheral blood mononuclear cells, or bone marrow cells. Amphotropic envelope sequences could not be detected in blood or bone marrow cells by Southern blotting or the polymerase chain reaction. Antibodies directed against the p30 and gp70 viral antigens were detected by Western blot and immunoprecipitation. The animals remain alive and well. Our findings suggest that primates can clear murine amphotropic retroviruses even when exposure occurs during a time of severe immunosuppression.
Cornetta
K
K
Molecular Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
Morgan
R A
RA
Gillio
A
A
Sturm
S
S
Baltrucki
L
L
O'Reilly
R
R
Anderson
W F
WF
D000243:Adenosine Deaminase / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D001769:Blood / Q000382:microbiology; D015139:Blotting, Southern; D015153:Blotting, Western; D001853:Bone Marrow / Q000382:microbiology; D016026:Bone Marrow Transplantation; D004279:DNA, Viral / Q000032:analysis; D015752:Genes, env; D005818:Genetic Engineering; D005822:Genetic Vectors; D016867:Immunocompromised Host; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008252:Macaca fascicularis / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics; D012189:Retrospective Studies; D012190:Retroviridae / Q000235:genetics; D014766:Viremia; D014779:Virus Replication
D000914:Antibodies, Viral; D004279:DNA, Viral; D011994:Recombinant Proteins; D000243:Adenosine Deaminase