Molecular cloning and sequencing of a cDNA encoding a human alpha 1A adrenergic receptor.
DNA from a rat hippocampus cDNA library and sets of highly degenerate oligonucleotide primers directed toward conserved regions of previously cloned G-protein receptors were used in the polymerase chain reaction to selectively amplify and clone new members of this gene family. A human hippocampus cDNA library was screened with a 610 base pair fragment generated by PCR and a cDNA clone, H318/3, was isolated. The deduced amino acid sequence of this clone encoded a protein of 501 amino acids that showed strong sequence homology to previously cloned G-protein receptors. Nucleotide sequence analysis revealed clone H318/3 was 78% homologous to a rat alpha 1A adrenergic receptor with homology being 95% when comparisons were made in the region that lies between the first to the seventh transmembrane domains. Based on this high degree of sequence homology, we conclude that clone H318/3 represents a cDNA for a human alpha 1A adrenergic receptor.
Bruno
J F
JF
Department of Medicine, State University of New York, Stony Brook 11794.
Whittaker
J
J
Song
J F
JF
Berelowitz
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D006624:Hippocampus / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D009838:Oligodeoxyribonucleotides; D011942:Receptors, Adrenergic, alpha; D004247:DNA