Effects of blood flow on lung ACE kinetics: evidence for microvascular recruitment.
The parameter Amax/Km (product of reactant enzyme mass in perfused microvessels and the constant kcat/Km), calculated from in vivo assays of pulmonary endothelial ectoenzymes (e.g., angiotensin-converting enzyme, ACE), can provide estimates of the perfused pulmonary microvascular surface area (PMSA) in the absence of enzyme dysfunction. We examined the relationship between PMSA and pulmonary blood flow (Qb) in anesthetized rabbits placed on total heart bypass, using [3H]benzoyl-Phe-Ala-Pro (BPAP) as the ACE substrate. When Qb was increased from 250 to 1,100 ml/min, at zone 3 conditions, pulmonary arterial pressure increased, pulmonary vascular resistance (PVR) decreased, and Amax/Km increased linearly, reflecting increasing PMSA. When only the left lung was perfused, increasing Qb from 250 to 636 +/- 17 ml/min (the last value representing fully recruited and/or distended vascular bed), PVR decreased, while Amax/Km increased. When Qb was further increased to 791 +/- 44 ml/min, both PVR and Amax/Km remained unchanged, confirming the lack of additional changes in PMSA. We conclude that Amax/Km provides a sensitive indication of PMSA, because it 1) increases with increasing Qb and decreasing PVR, 2) reaches a maximum at Qb values that correspond to the minimal values in PVR, and 3) like PVR, did not change with further increases in Qb. Compared with predicted changes in PMSA produced by either microvascular recruitment alone or distension alone, our data indicate that recruitment is a larger contributor to the observed increase in PMSA.
Toivonen
H J
HJ
Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta 30912-2300.
Catravas
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure; D001810:Blood Volume; D007700:Kinetics; D008168:Lung / Q000201:enzymology*; D008833:Microcirculation / Q000033:anatomy & histology / Q000502:physiology; D008954:Models, Biological; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D011652:Pulmonary Circulation / Q000502:physiology*; D011817:Rabbits; D013379:Substrate Specificity; D014655:Vascular Resistance
D009842:Oligopeptides; C029689:benzoylphenylalanyl-alanyl-proline; D007703:Peptidyl-Dipeptidase A