[A comparative evaluation of 2 methods for cloning human lung tumors].
Clonogenic capacity of tumor cells from 59 human lung cancer specimens was investigated in Petri dishes (24 specimens) and in capillaries (40 specimens), and parallel cloning was done in 5 cases. The results were as follows: (a) bacterial contamination in capillaries was noted less frequently than in Petri dishes (12.5 vs. 25%); (b) cells did not form colonies in capillaries in 42.5% and in Petri dishes in 25%; (c) colony-forming effectiveness in capillaries was 3.46-fold higher than that in Petri dishes; (d) the amount of tumor cells for analysis in capillaries is 10-fold less than that for analysis in Petri dishes. However, the absolute number of colonies, indispensable for assessment of tumor sensitivity to antitumor action (not less than 15 colonies per capillary or Petri dish) was obtained in 67% in Petri dishes and in 33% only in capillaries.
Sviridova
I K
IK
Moroz
I A
IA
Sergeeva
N S
NS
D000230:Adenocarcinoma / Q000473:pathology*; D018288:Carcinoma, Small Cell / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002999:Clone Cells / Q000166:cytology; D003114:Colony-Forming Units Assay; D003470:Culture Media; D003584:Cytological Techniques / Q000295:instrumentation; D005069:Evaluation Studies as Topic; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000166:cytology
D003470:Culture Media
Sravnitel'naia otsenka dvukh sposobov klonirovaniia opukholeĭ legkogo cheloveka.