High-performance liquid chromatographic analysis of idarubicin and fluorescent metabolites in biological fluids.
A specific, sensitive, and reliable high-performance liquid chromatographic (HPLC) method for the determination of idarubicin (IDA) and its known fluorescent metabolites idarubicinol (IDAol) and 4-demethoxy-daunomycinone (AG1) in biological fluids (human plasma and urine) was developed and tested. Plasma samples were solid-phase-extracted (C18 bonded silica cartridges). Complete separation of unchanged drugs and metabolites was achieved on a Cyanopropyl chromatographic column (25 cm x 4.6 mm inside diameter; particle size, 5 microns) using fluorescence detection (excitation wavelength, 470 nm; emission wavelength, 580 nm). Sensitivity was better than 0.2 ng/ml for all analytes; rates of recovery of unchanged drug and metabolites were better than 84.5% (IDA), 80.3% (IDAol), and 83.9% (AG1). The interassay coefficient of variation was 6.5% for IDA, 5.8% for IDAol, and 9.8% for AG1. Mean intra-assay precision was 4.6% for IDA, 5.9% for IDAol, and 5.0% for AG1 at sample concentrations of above 1 ng/ml and 12.1% for IDA, 10.8% for IDAol, and 14.1% for AG1 at sample concentrations of below 1 ng/ml.
Camaggi
C M
CM
Department of Organic-Chemistry, University of Bologna, Italy.
Carisi
P
P
Strocchi
E
E
Pannuti
F
F
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003630:Daunorubicin / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood / Q000652:urine; D005453:Fluorescence; D006801:Humans; D015255:Idarubicin / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine; D012015:Reference Standards
C075993:4-demethoxydaunomycinone; C054440:idarubicinol; D015255:Idarubicin; D003630:Daunorubicin