title: Development of IL-2-independent feline lymphoid cell lines chronically infected with feline immunodeficiency virus: importance for diagnostic reagents and vaccines.
abstract: Two interleukin 2 (IL-2)-independent feline immunodeficiency virus (FIV) producer cell lines (FL-4 and FL-6) were produced by selecting cells from an IL-2-dependent culture of mixed peripheral blood lymphocytes infected with FIV. The new cell lines have been stable for over 1 year and spontaneously produce FIV with an average reverse transcriptase titer of 300,000 cpm/ml and an average sucrose gradient purified viral protein concentration of 1 mg/l. FIV produced from these cultures is highly infectious in vitro and in vivo. The FL-6 cell line was phenotyped as expressing the feline CD8 and Pan-T antigens, while the FL-4 cell line expressed the CD4, CD8, and Pan-T antigens. Both cell lines, however, express high levels of viral core and envelope proteins. Paraformaldehyde-inactivated whole virus and similarly inactivated whole-cell virus preparations induced a strong antibody response to core and envelope antigens in immunized cats. The establishment of FIV-producing feline IL-2-independent peripheral blood lymphocyte lines should be valuable for the development of FIV-diagnostic reagents and vaccines and also as a model for human acquired immunodeficiency syndrome vaccine development.
authors.affiliation: Department of Medicine, MS-1A, School of Veterinary Medicine, University of California, Davis 95616.
authors.forename: J K
authors.initials: JK
authors.lastname: Yamamoto
authors.forename: C D
authors.initials: CD
authors.lastname: Ackley
authors.forename: H
authors.initials: H
authors.lastname: Zochlinski
authors.forename: H
authors.initials: H
authors.lastname: Louie
authors.forename: E
authors.initials: E
authors.lastname: Pembroke
authors.forename: M
authors.initials: M
authors.lastname: Torten
authors.forename: H
authors.initials: H
authors.lastname: Hansen
authors.forename: R
authors.initials: R
authors.lastname: Munn
authors.forename: T
authors.initials: T
authors.lastname: Okuda
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D016089:Immunodeficiency Virus, Feline / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D007376:Interleukin-2 / Q000494:pharmacology; D007700:Kinetics; D008214:Lymphocytes / Q000382:microbiology*; D014776:Virus Cultivation
chemical_list: D007376:Interleukin-2