[Secretory IgA: quality control of the titration method].
In order to verify if the method for the titration of salivary secretory IgA (s-IgA) by the technical of radial immunodiffusion is trustworthy, have been carried out the precision tests in the series and among the series in two saliva samples with very different s-IgA concentrations, and precision and accuracy tests in the series and among the series using a solution with a known titre of IgA. The results of the first battery tests have shown a precision ranging from 93.04 to 95.43%. The results of the tests of the second battery have shown a precision ranging about 96% and an accuracy about 97%. The tested method is, therefore, sufficiently trustworthy.
Rosaschino
F
F
Istituto di Pediatria, Università di Milano.
Torre
R
R
Grisler
R
R
D005069:Evaluation Studies as Topic; D006801:Humans; D005779:Immunodiffusion / Q000379:methods / Q000592:standards*; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D011786:Quality Control; D012463:Saliva / Q000737:chemistry / Q000276:immunology*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012680:Sensitivity and Specificity
D007071:Immunoglobulin A, Secretory; D012471:Salivary Proteins and Peptides
IgA secretorie: controllo di qualità della metodica di titolazione.