Specific cholinergic destruction in the basal magnocellular nucleus and impaired passive avoidance behavior of rodents.
A nerve growth factor (NGF)-diphtheria toxin conjugate (NGDT) was found to selectively abolish or depress the activity of NGF receptor-bearing cholinergic neurons of the basal magnocellular nucleus (BMN). Bilateral cortical injections of NGDT impaired the retention of passive avoidance behavior in mice. A memory deficit was also revealed when cortical injections of NGDT were administered after the acquisition of a passive avoidance response. Thus, retrograde destruction of BMN cholinergic neurons by the cortical injection of NGDT interfered with both learning and memory processes. The animal model outlined here should be useful in analyzing the pathogenesis of Alzheimer's disease and the functions of the cholinergic system in the BMN.
Imaizumi
K
K
Department of Neuroanatomy, Osaka University Medical School, Japan.
Kudo
Y
Y
Shiosaka
S
S
Lee
Y
Y
Ikeda
M
M
Ohta
H
H
Matano
S
S
Shoji
M
M
Honjoh
T
T
Tohyama
M
M
D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology*; D001522:Behavior, Animal / Q000502:physiology*; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004167:Diphtheria Toxin / Q000494:pharmacology; D006651:Histocytochemistry; D007150:Immunohistochemistry; D007267:Injections; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000502:physiology*; D051379:Mice; D009414:Nerve Growth Factors / Q000378:metabolism / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism; D010275:Parasympathetic Nervous System / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor
D004167:Diphtheria Toxin; D009414:Nerve Growth Factors; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D002795:Choline O-Acetyltransferase