title: Activation of the catalytic core of a group I intron by a remote 3' splice junction.
abstract: Over 1000 nucleotides may separate the ribozyme core of some group I introns from their 3' splice junctions. Using the sunY intron of bacteriophage T4 as a model system, we have investigated the mechanisms by which proximal splicing events are suppressed in vitro, as well as in vivo. Exon ligation as well as cleavage at the 5' splice site are shown to require long-range pairing between one of the peripheral components of the ribozyme core and some of the nucleotides preceding the authentic 3' splice junction. Consistent with our three-dimensional modeling of the entire sunY ribozyme, we propose that this novel interaction is necessary to drive 5' exon-core transcripts into an active conformation. A requirement for additional stabilizing interactions, either RNA-based or mediated by proteins, appears to be a general feature of group I self-splicing. A role for these interactions in mediating putative alternative splicing events is discussed.
authors.affiliation: Centre de Génétique Moléculaire du Centre National de la Recherche Scientifique (CNRS), Laboratoire Associè à l'Université Pierre et Marie Curie, Gif-sur-Yvette, France.
authors.forename: F
authors.initials: F
authors.lastname: Michel
authors.forename: L
authors.initials: L
authors.lastname: Jaeger
authors.forename: E
authors.initials: E
authors.lastname: Westhof
authors.forename: R
authors.initials: R
authors.lastname: Kuras
authors.forename: F
authors.initials: F
authors.lastname: Tihy
authors.forename: M Q
authors.initials: MQ
authors.lastname: Xu
authors.forename: D A
authors.initials: DA
authors.lastname: Shub
mesh_terms: D001483:Base Sequence; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D007438:Introns / Q000235:genetics / Q000502:physiology*; D008274:Magnesium / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics; D012326:RNA Splicing / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013604:T-Phages / Q000235:genetics
chemical_list: D016337:RNA, Catalytic; D012333:RNA, Messenger; D008274:Magnesium