title: Immunocytochemical demonstration of Na+,K(+)-ATPase in internodal axolemma of myelinated fibers of rat sciatic and optic nerves.
abstract: We used postembedding electron microscopic immunocytochemistry with colloidal gold to determine the ultrastructural distribution of Na+,K(+)-ATPase in the sciatic and optic nerves of the rat. Using a polyclonal antiserum raised against the denatured catalytic subunit of brain Na+,K(+)-ATPase, we found immunoreactivity along the internodal axolemma of myelinated fibers in both nerves. This antiserum did not produce labeling of nodal axolemma. These results suggest that an important site of energy-dependent sodium-potassium exchange is along the internodal axolemma of myelinated fibers in the mammalian CNS and PNS and that there may be differences between the internodal and nodal forms of the enzyme.
authors.affiliation: Neurology Research Laboratory, V.A. Medical Center, Ann Arbor, MI 48105.
authors.forename: M
authors.initials: M
authors.lastname: Mata
authors.forename: D J
authors.initials: DJ
authors.lastname: Fink
authors.forename: S A
authors.initials: SA
authors.lastname: Ernst
authors.forename: G J
authors.initials: GJ
authors.lastname: Siegel
mesh_terms: D000818:Animals; D001369:Axons / Q000201:enzymology*; D015153:Blotting, Western; D007120:Immunochemistry; D008297:Male; D008854:Microscopy, Electron; D009413:Nerve Fibers, Myelinated / Q000201:enzymology* / Q000648:ultrastructure; D009900:Optic Nerve / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000201:enzymology* / Q000648:ultrastructure; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D000254:Sodium-Potassium-Exchanging ATPase