Isolation of cell membrane for epidermal growth factor receptor studies.
The cell membrane isolation procedure we developed here can be scaled up from four to several hundred plates of cultured cells. Transmission electron microscopy, membrane marker enzyme analysis, binding study, EGF-dependent receptor autophosphorylation, and Western blots all demonstrate the biological activity of the purified cell membranes. The membrane purification procedure has been adapted by others in assessing EGF kinase activity and has been used for the purification of cell membranes from other types of cultured cells.
Lin
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PH
Selinfreund
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Wharton
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D000818:Animals; D015153:Blotting, Western; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D066246:ErbB Receptors / Q000302:isolation & purification / Q000378:metabolism*; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D008970:Molecular Weight; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011869:Radioligand Assay; D014461:Ultracentrifugation / Q000379:methods
D007202:Indicators and Reagents; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases