Phenotypic analysis of B-cells extracted from human periodontal disease tissue.
B-cells extracted from periodontal disease tissue were analyzed for the presence of activation markers using a range of monoclonal antibodies. In adult periodontitis (AP), 6% of B-cells expressed the IL-2 receptor (CD25) compared with 1-2% in peripheral blood and healthy or marginal gingivitis (H/MG) gingival B-cells. There was also an increase in the mean percentage of IgD-positive B-cells and a decrease in CD21 and CD22 expression. In both AP and H/MG lesions, 20-22% of the B-cells expressed CD23 compared with less than 5% in peripheral blood. As B-cells are activated by day 3 in culture and start differentiating into immunoglobulin-secreting cells by day 6, B-cell phenotypes were assayed at these times in this study. Following stimulation with the periodontopathic bacterium Porphyromonas gingivalis, the expression of CD23, CD21 and CD22 on B-cells extracted from AP lesions remained relatively constant over the 6-d culture period. However, with Fusobacterium nucleatum stimulation, there was a significant decrease in CD23, CD21 and CD22 expression after 3 d in culture, which corresponds to the activation time for B-cells. These results show that B-cells extracted from periodontal disease tissue display a range of activation markers and on stimulation, demonstrate differing responses to individual periodontopathic bacteria.
Gemmell
E
E
Department of Dentistry, University of Queensland, Australia.
Seymour
G J
GJ
D000328:Adult; D015703:Antigens, CD / Q000032:analysis*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D015815:Cell Adhesion Molecules; D005455:Fluorescent Antibody Technique; D016967:Fusobacterium nucleatum / Q000276:immunology*; D006801:Humans; D037102:Lectins; D008213:Lymphocyte Activation / Q000276:immunology; D010518:Periodontitis / Q000276:immunology* / Q000382:microbiology; D010641:Phenotype; D016966:Porphyromonas gingivalis / Q000276:immunology*; D011951:Receptors, Complement / Q000032:analysis; D017464:Receptors, Complement 3d; D011961:Receptors, Fc / Q000032:analysis; D017455:Receptors, IgE; D015375:Receptors, Interleukin-2 / Q000032:analysis; D051918:Sialic Acid Binding Ig-like Lectin 2
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C497867:CD22 protein, human; D015815:Cell Adhesion Molecules; D037102:Lectins; D011951:Receptors, Complement; D017464:Receptors, Complement 3d; D011961:Receptors, Fc; D017455:Receptors, IgE; D015375:Receptors, Interleukin-2; D051918:Sialic Acid Binding Ig-like Lectin 2