Expression of heat shock protein 70 and c-myc protein in human breast cancer: an immunohistochemical study.
The major heat shock protein, HSP70, protects cells from a variety of stressful stimuli, while c-myc protein allegedly stimulates the expression of HSP70 by transacting on the HSP70 promotor. The present study was aimed at correlating the expression of HSP70 with that of c-myc protein in benign and malignant breast lesions. Indirect immunoperoxidase and immunoblotting techniques using monoclonal antibodies were employed. Fresh frozen sections were prepared from five fibroadenomas and 59 breast carcinomas. Immunohistochemically, both substances were localized in the nuclei and/or cytoplasm of normal and neoplastic epithelial cells. The stromal cells were positive for c-myc protein but negative for HSP70. The expressions of HSP70 and c-myc protein were comparable to each other in the malignant cells from 37 (63%) carcinomas. In 17 (29%) carcinomas, c-myc protein expression predominated over that of HSP70, whereas in five (8%) carcinomas, HSP70 was predominant. Carcinomas with high-grade nuclear atypia often showed negative HSP70 staining, whereas tumors with nuclear HSP70 localization tended to accompany lower-grade nuclear atypia. A similar relation was also observed between c-myc protein expression and nuclear atypia of the tumor. All five fibroadenomas and most of the non-neoplastic epithelial cells adjacent to cancer showed strong reactivities with both substances. Immunoblot analysis for HSP70 revealed a clear, single band in the extract of tumors with strong HSP70 staining, but no, or only faint, bands were seen in the extract of immunohistochemically HSP70-negative carcinomas. The discrepancy between the expressions of each substance in a certain percentage of breast carcinomas may suggest the presence of HSP70 production mechanisms other than the c-myc protein-triggered promotor pathway.
Tauchi
K
K
Department of Pathology, Tokai University School of Medicine, Kanagawa.
Tsutsumi
Y
Y
Hori
S
S
Yoshimura
S
S
Osamura
R Y
RY
Watanabe
K
K
D000232:Adenofibroma / Q000737:chemistry / Q000648:ultrastructure; D001943:Breast Neoplasms / Q000737:chemistry* / Q000648:ultrastructure; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000737:chemistry / Q000648:ultrastructure; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D005260:Female; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis*
D006360:Heat-Shock Proteins; D016271:Proto-Oncogene Proteins c-myc