Negative-regulatory role of calmodulin in the expression of interferon-beta gene.
Addition of the calmodulin-antagonist, trifluoperazine (TFP), to human cell cultures producing biologically active IFN-beta in response to Sendai virus, results in a significant increase in IFN-beta production. This increase in IFN-beta production is observed 1 h after addition of TFP. The increase in IFN-beta production is correlated with increase in IFN-beta mRNA synthesis. Results suggest that calmodulin or calmodulin-dependent cellular process is involved in negative regulation of IFN-beta gene.
Lin
H Y
HY
Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York, Buffalo.
Thacore
H R
HR
D000818:Animals; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000276:immunology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics*; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014268:Trifluoperazine / Q000494:pharmacology
D002147:Calmodulin; D012333:RNA, Messenger; D014268:Trifluoperazine; D016899:Interferon-beta