1,25-Dihydroxyvitamin D3 increases epidermal growth factor receptor gene expression in BT-20 breast carcinoma cells.
In the breast cancer cell line BT-20 which displays a high number of Epidermal Growth Factor Receptors (EGF-R), we have previously observed that 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) at low concentration (0.1 nM) significantly reduces the proliferation rate while it upregulates the EGF binding capacity. The aim of the present investigation was to analyze EGF-R mRNA expression in 1,25-(OH)2D3 treated BT-20 cells. It is found that in cells treated for several days, the EGF-R mRNA levels are increased in relation to the dose from 0.01 to 1 nM. To investigate the time course of the response, cells received the drug only once and were harvested at different times. The data suggest that the stimulation of EGF-R mRNA expression is dose- and time-dependent. Therefore, the increased EGF binding capacity previously demonstrated can be related to the increase of EGF-R transcript levels.
Desprez
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PY
Laboratoire de Biologie Médicale, Centre de Lutte contre le Cancer Léon Bérard, Lyon, France.
Poujol
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D
Falette
N
N
Lefebvre
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MF
Saez
S
S
D015152:Blotting, Northern; D015139:Blotting, Southern; D001943:Breast Neoplasms; D002117:Calcitriol / Q000494:pharmacology*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D066246:ErbB Receptors / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D011061:Poly A; D066246:ErbB Receptors; D002117:Calcitriol