High-performance liquid chromatographic assay of L-alpha-glycerophosphorylcholine using a two-step enzymic conversion.
A high-performance liquid chromatographic method using an enzymic reactor for determination of L-alpha-glycerophosphorylcholine in pharmaceutical forms is described. The procedure includes incubation of L-alpha-glycerophosphorylcholine with glycerophosphorylcholine phosphodiesterase (EC 3.1.4.2), giving choline and glycerophosphate, and subsequent chromatography of choline with a post-column enzymic reactor and electrochemical detection. The results obtained show a close linearity of the whole assay from 2 to 150 nmol/ml L-alpha-glycerophosphorylcholine, the sensitivity being 2 pmol per 20 microliters of injected sample. The precision of the method in the analysis of L-alpha-glycerophosphorylcholine in pharmaceutical forms, ampoules and capsules, was 1.34 and 1.21%, respectively.
Abbiati
G
G
Biopharmaceutical Department, LPB Research Institute, Cinisello Balsamo, Italy.
Fossati
T
T
Arrigoni
M
M
Rolle
P
P
Dognini
G L
GL
Castiglioni
C
C
D002851:Chromatography, High Pressure Liquid; D005994:Glycerophosphates / Q000378:metabolism; D005997:Glycerylphosphorylcholine / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*
D005994:Glycerophosphates; D005997:Glycerylphosphorylcholine; D006861:Hydrogen Peroxide; D010727:Phosphoric Diester Hydrolases; C020677:glycerophosphocholine phosphodiesterase