Viral pathogenesis of a nephrotropic infectious bronchitis virus isolated from commercial pullets.
Infectious bronchitis was diagnosed in 3-to-4-week-old pullets from an outbreak in a commercial flock in California. The disease was characterized by head swelling, watery discharge from the eyes and nostrils, and urates in kidneys. Mortality ranged from 1.8% to 12.5% per week. The isolation of a coronavirus from a suspension of pooled kidneys from clinically ill chickens at the fifth passage in 10-day-old chicken embryos, gross and histologic renal lesions, and seroconversion by enzyme-linked immunosorbent assay in inoculated birds suggested that the virus isolated was a nephrotrophic strain of infectious bronchitis virus (IBV). The virus isolate was found to be a previously unrecognized serotype, based on virus neutralization tests performed in embryonated chicken eggs. Nephropathogenicity of the IBV isolate was confirmed by inoculation of the viral isolate into specific-pathogen-free chicks and demonstration of renal lesions. The isolation of nephrotropic strains of IBV has not been reported previously from poultry in California.
Kinde
H
H
California Veterinary Diagnostic Laboratory System, San Bernardino 92412.
Daft
B M
BM
Castro
A E
AE
Bickford
A A
AA
Gelb
J
J
Reynolds
B
B
D000818:Animals; D002140:California / Q000453:epidemiology; D002642:Chick Embryo; D002645:Chickens; D003333:Coronaviridae Infections / Q000453:epidemiology / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D004196:Disease Outbreaks; D005260:Female; D001351:Infectious bronchitis virus / Q000145:classification / Q000302:isolation & purification*; D007668:Kidney / Q000382:microbiology*; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology* / Q000473:pathology; D012703:Serotyping; D013047:Specific Pathogen-Free Organisms