Interferon transiently modulates intranuclear signalling system in erythroleukemia Friend cells.
The effect of human recombinant DNA interferon alpha type A on nuclear inositol lipids, diacylglycerol (DAG) and DNA metabolism has been investigated in Friend erythroleukemia cells. A transient enhancement of phosphatidylinositol (4,5) - bisphosphate (PIP2) phosphorylation together with an increase of diacylglycerol mass were observed in nuclei isolated from cells treated with interferon alpha for 90 min. At the same time, a marked reduction of DNA polymerase alpha activity was observed, suggesting a possible involvement of nuclear inositol fraction in the response of the cell nucleus to interferon treatment.
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Istituto di Morfologia Umana Normale, Università di Chieti, Italy.
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D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D007370:Interferon Type I / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010716:Phosphatidylinositols / Q000378:metabolism; D011994:Recombinant Proteins; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
C011439:1,2-diacylglycerol; D004075:Diglycerides; D007370:Interferon Type I; D010716:Phosphatidylinositols; D011994:Recombinant Proteins; D004257:DNA Polymerase II