Fluorescent and photoaffinity labeling probes for retinoic acid receptors.
A fluorescent probe for retinoid receptors (RARs) was designed and prepared. The probe consists of a retinoid moiety and a dansyl moiety, i.e., 2-[3-(5-dimethylaminonaphthalene-1-sulfonyl)- aminopropyl-1-oxy]-4-[(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2- naphthalenyl)carboxamido]benzoic acid: DAM-3. DAM-3 specifically bound RARs. Additionally, a photoreactive RAR fluorescent probe was designed and prepared, i.e., 2-[3-(5-azidonaphthalene- 1-sulfonyl)aminopropyl-1-oxy]-4-[(5,6,7,8-tetrahydro-5,5,8,8- tetramethyl-2-naphthalenyl)carboxamido]benzoic acid (ADAM-3). ADAM-3 irreversibly and specifically bound RARs using ultraviolet irradiation.
Shimazawa
R
R
Institute of Applied Microbiology, University of Tokyo, Japan.
Sanda
R
R
Mizoguchi
H
H
Hashimoto
Y
Y
Iwasaki
S
S
Tanaka
H
H
Kagechika
H
H
Shudo
K
K
D000345:Affinity Labels / Q000138:chemical synthesis*; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D003619:Dansyl Compounds; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D018168:Receptors, Retinoic Acid; D013329:Structure-Activity Relationship; D014212:Tretinoin / Q000378:metabolism*
D000345:Affinity Labels; D002352:Carrier Proteins; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D018168:Receptors, Retinoic Acid; D014212:Tretinoin