Quantitation of Mr 46000 and Mr 300000 mannose 6-phosphate receptors in human cells and tissues.
A highly specific enzyme-linked immunosorbent assay for quantitation of the Mr 46000 Da and Mr 300000 Da mannose 6-phosphate receptors was developed. The assay allows to detect ng amounts of human mannose 6-phosphate receptors. Analysis of human cells and tissues revealed significant differences in their contents of the two mannose 6-phosphate receptors, normalized for total cell protein. The ratio of the two mannose 6-phosphate receptors also differed among cells and tissues, suggesting that their steady state concentrations are regulated independently.
Wenk
J
J
Georg-August-University, Göttingen, Germany.
Hille
A
A
von Figura
K
K
D002460:Cell Line; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005347:Fibroblasts / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008360:Mannosephosphates; D008875:Middle Aged; D010920:Placenta / Q000737:chemistry; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface / Q000032:analysis*; D012680:Sensitivity and Specificity; D013154:Spleen / Q000737:chemistry; D013961:Thyroid Gland / Q000737:chemistry
D008360:Mannosephosphates; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface