title: [Oxidative inactivation of angiotensin-converting enzyme].
abstract: Hydrogen peroxide inactivates the purified human angiotensin-converting enzyme (ACE) in vitro; the inactivating effect of H2O2 is eliminated by an addition of catalase. The lung and kidney ACE are equally sensitive to the effect of hydrogen peroxide. After addition of oxidants (H2O2 alone or H2O2 + ascorbate or H2O2 + Fe2+ mixtures) to the membranes or homogenates of the lung, the inactivation of membrane-bound ACE is far less pronounced despite the large-scale accumulation of lipid peroxidation products. The marked inactivation of ACE in the membrane fraction (up to 55% of original activity) was observed during ACE incubation with a glucose:glucose oxidase:Fe2+ mixture. Presumably the oxidative potential of H2O2 in tissues in consumed, predominantly, for the oxidation of other components of the membrane (e.g., lipids) rather than for ACE inactivation.
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Sakharov
authors.forename: E A
authors.initials: EA
authors.lastname: Dukhanina
authors.forename: E A
authors.initials: EA
authors.lastname: Puchnina
authors.forename: S M
authors.initials: SM
authors.lastname: Danilov
authors.forename: V R
authors.initials: VR
authors.lastname: Muzykantov
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D001205:Ascorbic Acid / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D005290:Ferric Compounds / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008168:Lung / Q000201:enzymology; D010084:Oxidation-Reduction; D007703:Peptidyl-Dipeptidase A / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D013050:Spectrometry, Fluorescence
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000911:Antibodies, Monoclonal; D005290:Ferric Compounds; C024823:ferric sulfate; D006861:Hydrogen Peroxide; D002374:Catalase; D007703:Peptidyl-Dipeptidase A; D001205:Ascorbic Acid
vernacular_title: Oksidativnaia inaktivatsiia angiotenzinprevrashchaiushchego fermenta.