Morphological characterization of a newly established human osteosarcoma cell line, HS-Os-1, revealing its distinct osteoblastic nature.
A newly established human osteosarcoma cell line, HS-Os-1, from an osteoblastic tumor arising in the left humerus of an 11-year-old girl was morphologically characterized in vitro and in vivo. HS-Os-1 cells in a monolayer have been maintained for more than 2 years since the initial cultivation, and were round or polygonal in shape with marked pleomorphism. Their cytoplasm was strongly positive for specific markers of osteoblasts, such as alkaline phosphatase and osteocalcin. Tumors induced in nude mice by HS-Os-1 cell inoculation at passage 12 or 23 revealed typical histological features of osteoblastic osteosarcoma, similar to those observed in the original tumor, producing prominent osteoid matrix with calcification. Ultrastructurally, HS-Os-1 cells in vitro and tumor cells in vivo showed similar well-developed, markedly dilated rough endoplasmic reticulum, polysomes and microfilaments in their cytoplasm. Additionally, many collagen fibers associated with deposition of electron-dense material were detected in the stroma featuring osteoid matrix. Thus, the HS-Os-1 cell line was shown to exhibit its osteoblastic nature in vitro and in vivo, and therefore might become an extremely useful tool for various pathomorphological investigations on human osteosarcomas.
Second Department of Pathology, Kochi Medical School, Japan.
H
H
Sonobe
H
H
Mizobuchi
Y
Y
Manabe
M
M
Furihata
J
J
Iwata
T
T
Hikita
T
T
Oka
Y
Y
Ohtsuki
T
T
Goto
D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D002455:Cell Division; D002648:Child; D005260:Female; D006801:Humans; D006811:Humerus; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D010006:Osteoblasts / Q000473:pathology; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured