Distinct biochemical features of interferon gamma receptors on human T lymphocytes.
Recombinant human gamma interferon (IFN gamma) was used to study IFN gamma receptors (IFN gamma-R) on human CD4+ and CD8+ peripheral blood T lymphocytes. When cell-bound 125I-IFN gamma was cross-linked by disuccinimidyl suberate, the receptor-ligand complex migrated under nonreducing conditions as major bands of 155 kD and 90 kD and a minor band of 65-70 kD. Under reducing conditions, only the 90 and 65 kD complexes were found; the 155 kD band was not seen. In contrast, complexes from WISH and Raji human lines exhibited a single band of a 125 kD under both reducing and nonreducing conditions. The molecular pattern of the receptor ligand complex on WISH cells was not altered when these cells were mixed with T lymphocytes during the extraction procedure. The results suggest that the IFN gamma receptor on T lymphocytes differs from those previously described.
Howard Hughes Medical Institute Laboratory, Baylor College of Medicine, Houston, Texas 77030.
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ElMasry
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Cook
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Rich
D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D002460:Cell Line; D003432:Cross-Linking Reagents; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000378:metabolism; D008970:Molecular Weight; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000378:metabolism; D017471:Receptors, Interferon; D016176:T-Lymphocyte Subsets / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology*
D003432:Cross-Linking Reagents; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D007371:Interferon-gamma