Immunohistochemical studies of retinoblastoma.
Various immunohistochemical markers were detected in 27 cases of human retinoblastoma by immunohistochemical avidin-biotin-peroxidase complex and peroxidase-antiperoxidase techniques using mono- and polyclonal antibodies. The glial markers, glial fibrillary acidic protein and Leu-7, were detected in the natural retinal component and perivascular glial cells within the tumor mass. In 4 among 27 cases Leu-7 was positive in the glial cells which were interspersed randomly among tumor cells and not associated with blood vessels. The neural marker, neuron-specific enolase, stained strongly positive in undifferentiated tumor cells and weakly positive in rosettes and fleurettes in most of the cases (21/27). Opsin, the photoreceptor marker, which is present only in the outer segment of photoreceptor cells, was detected in tumor cells of fleurettes in one case and also in rosettes in 6 cases. These results support the view that retinoblastoma has a predominantly neuronal nature with significant photoreceptor-like differentiation and rarely glial differentiation.
Department of Ophthalmology, Ichihara Hospital, Teikyo University School of Medicine, Chiba, Japan.
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XL
Sun
T
T
Yokoyama
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K
Minoda
A
A
Sakuma
D000328:Adult; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism; D019393:Arrestin; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018959:CD57 Antigens; D002648:Child; D002675:Child, Preschool; D005134:Eye Neoplasms / Q000378:metabolism*; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007223:Infant; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008297:Male; D016900:Neurofilament Proteins; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D012175:Retinoblastoma / Q000378:metabolism*; D017299:Rod Opsins
D000911:Antibodies, Monoclonal; D000941:Antigens; D000943:Antigens, Differentiation; D019393:Arrestin; D014408:Biomarkers, Tumor; D018959:CD57 Antigens; D005136:Eye Proteins; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D017299:Rod Opsins; D010751:Phosphopyruvate Hydratase