Determination of a molecular map position for Hyp using a new interspecific backcross produced by in vitro fertilization.
We have established a Mus spretus/Mus musculus domesticus interspecific backcross segregating for two X-linked mutant genes, Ta and Hyp, using in vitro fertilization. The haplotype of the recombinant X chromosome of each of 241 backcross progeny has been established using the X-linked anchor loci Otc, Hprt, Dmd, Pgk-1, and Amg and the additional probes DXSmh43 and Cbx-rs1. The Hyp locus (putative homologue of the human disease gene hypophosphatemic rickets, HYP) has been incorporated into the molecular genetic map of the X chromosome. We show that the most likely gene order in the distal portion of the mouse X chromosome is Pgk-1-DXSmh43-Hyp-Cbx-rs1-Amg, from proximal to distal. The distance in centimorgans (mean +/- SE) between DXSmh43 and Hyp was 2.52 +/- 1.4 and that between Hyp and Cbx-rs1 was 1.98 +/- 1.39. Thus closely linked flanking markers for the Hyp locus that will facilitate the molecular characterization of the gene itself have been defined.
Section of Comparative Biology, MRC Clinical Research Centre, Harrow, Middlesex, United Kingdom.
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D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D015139:Blotting, Southern; D002118:Calcium / Q000097:blood; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D015342:DNA Probes / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro; D008040:Genetic Linkage / Q000235:genetics*; D006801:Humans; D007015:Hypophosphatemia, Familial / Q000235:genetics*; D008297:Male; D051379:Mice; D009154:Mutation / Q000235:genetics; D010710:Phosphates / Q000097:blood; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic / Q000235:genetics; D014960:X Chromosome
D015342:DNA Probes; D010710:Phosphates; D000469:Alkaline Phosphatase; D002118:Calcium