Polypeptides synthesized in rabbit cells infected with murine herpesvirus (MHV): a comparison of proteins specified by various MHV strains.
Polypeptides synthesized in rabbit embryo fibroblasts (REF) infected with six isolates of murine herpesvirus (MHV) coming from different localities in CSFR were compared by electrophoresis (SDS-PAGE). Altogether 28 virus-coded polypeptides with apparent molecular weights ranging from 240 kD to 16 kD were identified. While isolates MHV-60, MHV-72, and MHV-76 had identical polypeptide profiles, the isolates MHV-68 and MHV-78 lacked a polypeptide with apparent molecular weight of 46 kD, the rest of polypeptides being identical as well. The polypeptide profile of MHV Sumava A.f. was most different from that of other MHV isolates. This result is in agreement with some different biological properties of this isolate. At a high multiplicity of infection (40 TCID50 per cell) first polypeptides with apparent molecular weights 125 kD and 84 kD were detected by 5-9 hr post-infection (p.i.). All other proteins appeared 14-18 p.i.
Department of Microbiology and Virology, Faculty of Natural Sciences of Comenius University, Bratislava, Czecho-Slovakia.
M
M
Reichel
J
J
Matis
J
J
Lesso
M
M
Stanceková
D000818:Animals; D002478:Cells, Cultured; D006564:Herpesviridae / Q000145:classification / Q000378:metabolism* / Q000502:physiology; D007700:Kinetics; D010452:Peptide Biosynthesis; D011817:Rabbits; D013045:Species Specificity; D014764:Viral Proteins / Q000096:biosynthesis*
D014764:Viral Proteins