Association of the v-crk oncogene product with phosphotyrosine-containing proteins and protein kinase activity.
An antiserum specific for P47gag-crk, the oncogene product of avian sarcoma virus CT10, was used to investigate possible crk-binding proteins. In in vitro kinase assays, four proteins were phosphorylated in anti-crk immunoprecipitates. Tyrosine, serine, and threonine residues were phosphorylated. A number of tyrosine-phosphorylated proteins were identified in anti-crk immunoprecipitates of 32P-labeled cells, including the three major phosphotyrosine-containing proteins of CT10-infected cells. These three proteins also bound to bacterially synthesized crk protein. These results suggest that the crk transforming protein can stably associate with both endogenous kinases and cellular kinase substrates.
Rockefeller University, New York, NY 10021.
B J
BJ
Mayer
H
H
Hanafusa
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D002478:Cells, Cultured; D002642:Chick Embryo; D004926:Escherichia coli / Q000235:genetics; D005347:Fibroblasts / Q000201:enzymology; D050736:Oncogene Protein v-crk; D009857:Oncogenes; D010449:Peptide Mapping; D010748:Phosphopeptides / Q000302:isolation & purification; D010750:Phosphoproteins / Q000302:isolation & purification; D019000:Phosphotyrosine; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012191:Retroviridae Proteins / Q000235:genetics / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
D050736:Oncogene Protein v-crk; D010748:Phosphopeptides; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D012191:Retroviridae Proteins; C060731:p47 (gag-crk) protein, Avian sarcoma virus; D019000:Phosphotyrosine; D014443:Tyrosine