Preliminary crystallographic analysis of the Fab fragment of an antibody against HIV gp120.
Single crystals of the Fab fragment of a murine monoclonal antibody BAT123 (IgG1, kappa) raised against a dominant neutralizing determinant of gp120 of HIV that are suitable for X-ray structural analysis have been obtained. The thick prismatic plate crystals belong to space group P2(1)2(1)2 with unit cell dimensions of a = 177.42 A, b = 37.36 A and c = 73.30 A.
Vyas
M N
MN
Department of Biochemistry, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030.
Vyas
N K
NK
Liou
R S
RS
Chang
T W
TW
Quiocho
F A
FA
D000818:Animals; D000911:Antibodies, Monoclonal; D000939:Epitopes / Q000276:immunology; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments; D051379:Mice; D008807:Mice, Inbred BALB C; D014961:X-Ray Diffraction
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D007140:Immunoglobulin Fab Fragments