Sensitive and specific quantification of human genomic deoxyribonucleic acid (DNA) in forensic science specimens: casework examples.
We describe the forensic science application of a method for quantification of human genomic deoxyribonucleic acid (DNA). The two cases cited in this report involve DNA samples extracted from skin tissue and bloodstained clothing recovered from different crime scenes. High-molecular-weight DNA was recovered from both specimens, and the concentrations of these DNAs were estimated to be approximately 0.5 microgram/microL by ethidium bromide/agarose gel electrophoresis. Using the human-specific DNA probe p17H8 (locus D17Z1) to quantify the amount of human genomic DNA in these samples, it is shown that less than 1% of the DNA isolated from the skin tissue is of human origin and that the DNA isolated from the bloodstained clothing is effectively devoid of human DNA sequences. These case examples illustrate the need to quantify not only the total amount of DNA recovered from forensic casework material, but also the proportion of the DNA that is of human origin.
McMaster University, Hamilton, Ontario, Canada.
J S
JS
Waye
D
D
Michaud
J H
JH
Bowen
R M
RM
Fourney
D000328:Adult; D001237:Asphyxia; D001345:Autoradiography; D001801:Blood Stains; D015139:Blotting, Southern; D002056:Burns; D002648:Child; D004247:DNA / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D006708:Homicide; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D012867:Skin / Q000737:chemistry*
D004247:DNA