A quantitative assay for tyrosine sulfation and tyrosine phosphorylation in peptides.
A method was developed to measure sulfation and phosphorylation of tyrosine in proteins after alkaline hydrolysis, ion-exchange chromatography, reaction with [3H]dinitrofluorobenzene and subsequent thin-layer chromatography. The method allows the detection of 10-20 pmol of modified tyrosine and was applied to determine the content of tyrosine-phosphate and -sulfate in fibrinogens, thyroglobulin, alpha-casein, cytochrome c and glyceraldehyde dehydrogenase.
Robert Koch-Institut des Bundesgesundheitsamtes, Berlin.
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H
Blode
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T
Heinrich
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H
Diringer
D055598:Chemical Phenomena; D002621:Chemistry; D010455:Peptides / Q000032:analysis*; D019000:Phosphotyrosine; D012015:Reference Standards; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D010455:Peptides; D019000:Phosphotyrosine; C005662:tyrosine O-sulfate; D014443:Tyrosine