The value of electronmicroscopy and immunohistochemistry in the diagnosis of soft tissue sarcomas: a study of 200 cases.
Two hundred soft tissue sarcomas, accrued consecutively over a 4-year period, were examined by light and electronmicroscopy and by routine immunohistochemistry. The commonest tumour type was malignant fibrous histiocytoma. Fibrosarcoma, composed only of fibroblasts, was diagnosed in only one case; three others, composed also of myofibroblasts, could be regarded as fibrosarcomas or myofibrosarcomas. Immunohistochemistry was of most value in the diagnosis of rhabdomyosarcoma, leiomyosarcoma, malignant peripheral nerve sheath tumour of Schwann cell type and epithelioid sarcoma. Electronmicroscopy was of most use for the diagnosis of malignant peripheral nerve sheath tumour of perineurial cell type and marker-negative monophasic synovial sarcoma, and for confirming malignant fibrous histiocytoma. Fifteen of 19 marker-negative spindle cell tumours (79%) were diagnosable by electronmicroscopy. A combination of techniques resulted in a specific diagnosis in 193 cases (96.5%). The routine use of electronmicroscopy in sarcoma diagnosis can improve accuracy of diagnosis, establish the true frequency of marker-positivity for each ultrastructurally confirmed tumour type and minimise the number of unclassifiable cases.
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Department of Histopathology, Royal Marsden Hospital, London, UK.
D014408:Biomarkers, Tumor / Q000378:metabolism; D003951:Diagnostic Errors; D005069:Evaluation Studies as Topic; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D008854:Microscopy, Electron; D012509:Sarcoma / Q000175:diagnosis* / Q000378:metabolism / Q000648:ultrastructure; D012983:Soft Tissue Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000648:ultrastructure
D014408:Biomarkers, Tumor; D007633:Keratins