Effects of antineoplastic phospholipids on parameters of cell differentiation in U937 cells.
The proliferation of the human promonocytic leukemia cell line U937 is inhibited by several ether lipids, ether lipid analogues and by phorbol esters. An early effect of this retardation of cell growth is the induction of a basic chromosomal protein, histone H1(0). Northern blot analysis of H1(0) mRNA levels reveals an increase of the mRNA concentration within a few hours after addition of hexadecylphosphocholine and 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine. This early effect on the synthesis of a subtype of H1 proteins precedes the expression of several parameters of the monocytic differentiation of U937 cells.
Hochhuth
C
C
Institut für Biochemie, Abteilung Molekularbiologie, Göttingen, Federal Republic of Germany.
Berkovic
D
D
Eibl
H
H
Unger
C
C
Doenecke
D
D
D000954:Antigens, Surface / Q000032:analysis; D000970:Antineoplastic Agents / Q000276:immunology / Q000494:pharmacology*; D015152:Blotting, Northern; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D015342:DNA Probes; D005233:Fatty Alcohols / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006657:Histones / Q000096:biosynthesis; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy*; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology; D010742:Phospholipid Ethers / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology*; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000954:Antigens, Surface; D000970:Antineoplastic Agents; D015342:DNA Probes; D005233:Fatty Alcohols; D006657:Histones; D008244:Lysophosphatidylcholines; D010703:Phorbol Esters; D010742:Phospholipid Ethers; D010743:Phospholipids; D012333:RNA, Messenger; D010767:Phosphorylcholine; C066233:hexadecylphospho(N,N,N-trimethylamino)hexanol; C006065:We 201; C026659:edelfosine; C039128:miltefosine; D012313:RNA; D013755:Tetradecanoylphorbol Acetate