An immunoglobulin light chain from a lupus-prone mouse induces autoantibodies in normal mice.
Autoantibodies against the 70-kD U1 RNP nucleoprotein autoantigen and DNA were elicited in normal BALB/c mice with a purified Ig light chain. This light chain, derived from a lupus-prone MRL-lpr/lpr mouse, has two distinctive properties: it contains an idiotypic marker recognized by a monoclonal MRL-lpr/lpr anti-snRNP autoantibody, and the amino acid sequence of its third hypervariable region (CDR3) is homologous to a sequence in an antigenic region of the 70-kD U1 RNP polypeptide. The results demonstrate that an Ig idiotype that mimics an autoantigen can induce autoimmunization.
Puccetti
A
A
Department of Medicine, Tufts University School of Medicine, Boston, MA 02111.
Koizumi
T
T
Migliorini
P
P
André-Schwartz
J
J
Barrett
K J
KJ
Schwartz
R S
RS
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000096:biosynthesis* / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D001483:Base Sequence; D001667:Binding, Competitive; D004247:DNA / Q000276:immunology; D004277:DNA, Single-Stranded / Q000276:immunology; D000939:Epitopes; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000008:administration & dosage / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011817:Rabbits; D012261:Ribonucleoproteins / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid; D055517:snRNP Core Proteins
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D001324:Autoantigens; D004277:DNA, Single-Stranded; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D012261:Ribonucleoproteins; D055517:snRNP Core Proteins; D004247:DNA