Human granulosa cells contain insulin-like growth factor-binding protein (IGF BP-1) mRNA.
Previous studies have demonstrated expression of insulin-like growth factor-binding protein (IGF BP-1) in secretory and decidualized endometrium, in adult and fetal liver, and in HepG2 liver cancer cells. We have studied the expression of IGF BP-1 in various types of ovarian neoplasias, normal ovary, and granulosa cells from hyperstimulated human ovarian follicles by RNA blot hybridization. A single 1.6 kb mRNA species, similar to that present in human decidua, was identified in poly(A)RNA-containing preparations of granulosa cells and of a borderline malignant ovarian cystadenoma. This finding verifies the postulated production of IGF BP-1 by the human ovary.
Department I of Obstetrics and Gynecology, University Central Hospital, Helsinki, Finland.
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R
Koistinen
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Suikkari
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A
Tiitinen
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Kontula
M
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Seppälä
D000328:Adult; D000368:Aged; D002352:Carrier Proteins / Q000235:genetics*; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D018969:Insulin-Like Growth Factor Binding Proteins; D008875:Middle Aged; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010051:Ovarian Neoplasms / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*
D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger