Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line.
1. The whole-cell configuration of the patch-clamp recording technique was used to characterize the electrophysiological properties of CRI-G1 insulin-secreting cells. 2. Current-clamp recordings demonstrated the excitable nature of these cells. 3. Voltage-clamp recordings revealed the presence of an inward Na+ current, an inward Ca2+ current and a delayed outward K+ conductance. 4. The electrophysiological properties of CRI-G1 closely resemble those of pancreatic beta-cells, thereby rendering this cell-line as a useful alternative to freshly isolated cells for the study of pancreatic beta-cell electrophysiology and pharmacology.
Department of Pharmacology, University of Cambridge, U.K.
R Z
RZ
Kozlowski
N C
NC
Sturgess
C N
CN
Hales
M L
ML
Ashford
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002460:Cell Line; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D008954:Models, Biological; D051381:Rats
D007328:Insulin