[The separation of some racemic beta-blockers and alpha-sympathomimetics with HPLC following derivatization].
Five beta-adrenergic blocking agents and two alpha-sympathicomimetics were separated after derivatization with (R)-(-)-1-(Naphth-1-yl)-ethylisocyanate (NEIC). For two of these analytes the separation was successful also after reaction with (R)-(+)-1-phenylethylisocyanate (PEIC) by using HPLC with RP-18 phases and a water-methanolic eluent by UV-detection on 254 nm. After reaction with (1S)-(+)-campher-10-sulfonylchloride (CSC) an acceptable separation of norphenylephedrine also was possible. Analytes which can be derivated with NEIC and PEIC give no reaction with CSC (and vice versa).
Fachbereich Pharmazie, Ernst-Moritz-Arndt-Universität Greifswald.
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Jira
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Vogt
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Beyrich
D000316:Adrenergic alpha-Agonists / Q000737:chemistry / Q000302:isolation & purification*; D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003485:Cyanates; D007202:Indicators and Reagents; D017953:Isocyanates; D009281:Naphthalenes; D013237:Stereoisomerism
D000316:Adrenergic alpha-Agonists; D000319:Adrenergic beta-Antagonists; D003485:Cyanates; D007202:Indicators and Reagents; D017953:Isocyanates; D009281:Naphthalenes; C020790:phenylethyl isocyanate; C054308:1-(1-naphthyl)ethyl isocyanate
Zur Trennung einiger racemischer beta-blocker und alpha-Sympathikomimetika durch HPLC nach Derivatisierung.