The relationship between the hypokalaemic response to adrenaline, beta-adrenoceptors, and Na(+)-K+ pumps in skeletal and cardiac muscle membranes in the rabbit.
The hypokalaemic response to adrenaline and the involvement of beta-adrenoceptors and Na(+)-K+ pumps were investigated in control rabbits and animals chronically pretreated with adrenaline. The hypokalaemic response to acute intravenous infusion of adrenaline was significantly reduced when rabbits were chronically pretreated with adrenaline for 10 days. Chronic pretreatment of rabbits with adrenaline significantly reduced the densities for [125I]cyanopindolol and [3H]ouabain binding sites in skeletal muscle and heart. Furthermore, there was a strong positive correlation (r = 0.97, p less than 0.001) between the Bmax for ICYP and [3H]ouabain, in the rabbit heart. Ouabain-sensitive 86Rb uptake and the activity of 3-O-methylfluorescein phosphate phosphatase were used to assess the function of the Na(+)-K+ pump in skeletal and cardiac muscle. There was no significant difference in these functional indices of the Na(+)-K+ pump between the control and adrenaline-pretreated animals, in skeletal or cardiac muscle. Thus, downregulation of the [3H]ouabain binding sites did not appear to be accompanied by reduced function of the Na(+)-K+ pump. Additional investigations are required to confirm further the dissociation between the function of the pump and the ouabain binding sites.
University Department of Materia Medica, Stobhill General Hospital, Glasgow, Scotland.
M S
MS
Elfellah
J L
JL
Reid
D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology*; D005452:Fluoresceins / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D007008:Hypokalemia / Q000097:blood / Q000139:chemically induced*; D066298:In Vitro Techniques; D020120:Iodocyanopindolol; D008297:Male; D008566:Membranes / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010042:Ouabain; D010869:Pindolol / Q000031:analogs & derivatives; D011188:Potassium / Q000097:blood / Q000378:metabolism*; D011817:Rabbits; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012414:Rubidium Radioisotopes; D012964:Sodium / Q000378:metabolism*
C032079:3-O-methylfluorescein phosphate; D005452:Fluoresceins; D011943:Receptors, Adrenergic, beta; D012414:Rubidium Radioisotopes; D010042:Ouabain; D020120:Iodocyanopindolol; D012964:Sodium; D010869:Pindolol; D011188:Potassium; D004837:Epinephrine