Repeated measurement of spontaneous and clastogen-induced sister-chromatid exchange.
Sister-chromatid exchanges (SCE), both spontaneous and chemically-induced [bleomycin (BLM), mitomycin-C (MMC), streptonigrin (SN), and 4-nitroquinoline-1-oxide (4NQO)], were studied in the lymphocytes of 24 normal individuals on 2 or 3 different occasions, separated by periods of up to 2 years. For all BLM-induced SCEs, the variation in SCE frequency among the samples from a single individual was significantly greater than the variation between replicate cultures on a given day. These results raise questions concerning the validity of conclusions based on a single observation of chemically-induced SCEs.
Schwartz
S
S
Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.
Astemborski
J A
JA
Budacz
A P
AP
Boughman
J A
JA
Wasserman
S S
SS
Cohen
M M
MM
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D001761:Bleomycin / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology*; D012016:Reference Values; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013308:Streptonigrin / Q000494:pharmacology
D008937:Mitomycins; D009153:Mutagens; D001761:Bleomycin; D013308:Streptonigrin; D016685:Mitomycin; D015112:4-Nitroquinoline-1-oxide