Identification of a ligand for the c-kit proto-oncogene.
We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proliferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit protooncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.
Department of Experimental Hematology, Immunex Corporation, Seattle, Washington 98101.
D E
DE
Williams
J
J
Eisenman
A
A
Baird
C
C
Rauch
K
K
Van Ness
C J
CJ
March
L S
LS
Park
U
U
Martin
D Y
DY
Mochizuki
H S
HS
Boswell
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D004261:DNA Replication; D016298:Hematopoietic Cell Growth Factors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008024:Ligands; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019009:Proto-Oncogene Proteins c-kit; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D019089:Stem Cell Factor; D014158:Transcription, Genetic
D016298:Hematopoietic Cell Growth Factors; D008024:Ligands; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D019089:Stem Cell Factor; D011505:Protein-Tyrosine Kinases; D019009:Proto-Oncogene Proteins c-kit