Repression of the alpha-fetoprotein gene promoter by progesterone and chimeric receptors in the presence of hormones and antihormones.
Using transient transfection assays, we showed that repression of the alpha-fetoprotein promoter by intact and deletion mutants of the progesterone receptor and by chimeric progesterone/glucocorticoid-estrogen receptors in the presence of their cognate hormones was closely correlated with their ability to bind to a progesterone/glucocorticoid-responsive element. This negative regulation was also observed in the presence of antihormones, providing evidence that receptor-antihormone complexes can bind to their responsive elements in vivo.
Turcotte
B
B
Unité 184 de Biologie Moléculaire et de Génie Génétique de l'Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine, Institut de Chimie Biologique, Strasbourg, France.
Meyer
M E
ME
Bocquel
M T
MT
Bélanger
L
L
Chambon
P
P
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens; D002678:Chimera; D002872:Chromosome Deletion; D005796:Genes; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D011374:Progesterone / Q000494:pharmacology*; D011397:Promegestone / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011980:Receptors, Progesterone / Q000235:genetics / Q000502:physiology*; D015183:Restriction Mapping; D013629:Tamoxifen / Q000031:analogs & derivatives / Q000494:pharmacology; D014162:Transfection; D000509:alpha-Fetoproteins / Q000235:genetics*
D015345:Oligonucleotide Probes; D011980:Receptors, Progesterone; D000509:alpha-Fetoproteins; D013629:Tamoxifen; D015735:Mifepristone; D011374:Progesterone; D011397:Promegestone