[Determination of the primary structure of Eco-R1-fragment No. 5 of the rat tyrosine aminotransferase gene in the "Applied Biosystems" 370 automatic sequencer].
EcoRI-fragment No. 5 of the rat tyrosine aminotransferase gene containing exons K, L, intron 11, and a part of the 3'-nontranslatable region was digested with several restriction endonucleases (BspRI, Sau3A, BamHI, Ecl136II, AluI), the subfragments obtained were cloned into M13mp19 and sequenced using the Sanger technique with dye-labelled primers on the automated sequencer "Applied Biosystems", model 370A. The sequences were combined by means of a PC-GENE package and original programs to yield the primary structure (1064 b. p.) of the above fragment No. 5, adjacent to the previously sequenced EcoRI-fragment No. 4 (3677 b.p.).
S M
SM
Zelenin
V S
VS
Popova
I V
IV
Morozov
V I
VI
Tishkov
A M
AM
Egorov
N P
NP
Mertvetsov
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism*; D008969:Molecular Sequence Data; D051381:Rats; D014444:Tyrosine Transaminase / Q000235:genetics*
D014444:Tyrosine Transaminase; D015246:Deoxyribonuclease EcoRI
Opredelenie pervichnoĭ struktury Eco RI-fragmenta No. 5 gena tirozinaminotransferazy krysy na avtomaticheskom sekvenatore "Applied Biosystems" 370A.