Rapid vascular clearance of two strains of Junin virus in Calomys musculinus: selective macrophage clearance.
Clearance of Junin (JUN) virus strains with different virulence for Calomys musculinus (Cm) was followed using the Candid #1 virulent and CbaFHA 5069 attenuated strains. In addition, virulent virus albino mice (AM) were included as control host and Venezuelan equine encephalitis (VEE-VI) virus as control virus. The virus inoculum (Vo) and the blood samples (Vt) obtained at different times post-inoculation (p.i.) were titrated on Vero cells and the cleared plaque forming-units (PFU) were calculated as the log Vt/Vo. In Cm both JUN virus strains were cleared rapidly (within 5 min the Candid #1 strain and within 10 min the CbaFHA 5069 strain); meanwhile, VEE-VI virus could be recovered from blood until 30 min p. i. Furthermore, JUN and VEE-VI viruses showed the same behaviour in Am as in Cm. We conclude that the JUN virus strains of different virulence for Cm did not show differences in their clearance from the blood of these animals. Moreover, the rapid clearance observed was independent of the animal host and viral dose.
Instituto de Virologia, Universidad Nacional de Cordoba, Ciudad Universitaria, Argentina.
M S
MS
Contigiani
S I
SI
Medeot
G E
GE
Diaz
M S
MS
Sabattini
D000818:Animals; D006477:Arenaviruses, New World / Q000276:immunology* / Q000472:pathogenicity; D003411:Arvicolinae / Q000382:microbiology*; D006478:Hemorrhagic Fever, American / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D014709:Vero Cells / Q000382:microbiology; D010948:Viral Plaque Assay; D014765:Viral Vaccines; D014766:Viremia / Q000097:blood* / Q000382:microbiology; D014774:Virulence
D014765:Viral Vaccines