A comparative study of the effects of phebrol on the respiratory chains of rat liver, Biomphalaria glabrata and Oncomelania nosophora.
1. Phebrol (sodium 2,5-dichloro-4-bromophenol), a synthetic molluscicide against Oncomelania nosophora, showed a dual effect on rat liver submitochondria, acting as an uncoupler at low concentrations (approximately 10 microM) and an inhibitor of succinate-cytochrome c reductase at high concentrations. 2. Phebrol also inhibited the enzymes responsible for succinate-fumarate conversion, i.e. the succinate-cytochrome c reductase, fumarate reductase and NADH-cytochrome c reductase of the mitochondrial fraction from Biomphalaria glabrata. 3. Kinetic inhibition studies showed succinate-cytochrome c reductase of B. glabrata and O. nosophora to be more sensitive than that of rat liver toward phebrol. 4. Phebrol accumulated in whole tissues of B. glabrata and O. nosophora and had significant effects on the production of succinate, fumarate and malate by these snails. 5. On the basis of these results, the possible sites of inhibition by phebrol of snail respiratory chains are proposed.
Department of Parasitology, Juntendo University School of Medicine, Tokyo, Japan.
R
R
Furushima
S
S
Takamiya
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IV
Okochi
K
K
Kita
T
T
Aoki
H
H
Oya
D000818:Animals; D001700:Biomphalaria / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002733:Chlorophenols / Q000494:pharmacology*; D005650:Fumarates / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008293:Malates / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D008975:Molluscacides / Q000494:pharmacology*; D009245:NADH Dehydrogenase / Q000037:antagonists & inhibitors; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D012908:Snails / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D013384:Succinate Cytochrome c Oxidoreductase / Q000037:antagonists & inhibitors; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D014475:Uncoupling Agents / Q000494:pharmacology
D002733:Chlorophenols; D005650:Fumarates; D008293:Malates; D008975:Molluscacides; D013386:Succinates; D014475:Uncoupling Agents; C023970:2,5-dichloro-4-bromophenol; C030298:malic acid; C032005:fumaric acid; D019802:Succinic Acid; D013384:Succinate Cytochrome c Oxidoreductase; D013385:Succinate Dehydrogenase; D009245:NADH Dehydrogenase