[Marker histochemical demonstration of phosphoenolpyruvate at the light-optical and electron-microscopic levels].
Results of staining section of rat liver using colloidal gold-labeled pyruvate kinase are described. On light microscopic level, centrolobular localized hepatocytes displayed the most intense specific staining. With electron microscopic observations, phosphoenolpyruvate residues were seen over granular zones in the nuclei, over the cisternae of the granular ER, mitochondria, and hepatocyte microvilli.
A L
AL
Glazyrin
S I
SI
Kolesnikov
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D003102:Colloids; D006046:Gold; D017979:Gold Colloid; D006651:Histocytochemistry; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D008853:Microscopy; D008854:Microscopy, Electron; D010728:Phosphoenolpyruvate / Q000032:analysis / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling / Q000379:methods; D013379:Substrate Specificity
D015415:Biomarkers; D003102:Colloids; D017979:Gold Colloid; C058457:Aurodye; D010728:Phosphoenolpyruvate; D006046:Gold; D011770:Pyruvate Kinase
Markerno-gistokhimicheskoe vyiavlenie fosfoenolpiruvata na svetoopticheskom i élektronno-mikroskopicheskom urovniakh.