An analysis of the prolactin surge.
Daily sera from normally menstruating women analysed by radioimmunoassay (RIA) with prolactin kits from NIH showed midcycle prolactin elevations coinciding with LH surge. No such elevations were observed with Serono prolactin kit. Sera assayed both at NIH and Population Council laboratories confirmed the higher prolactin values measured by NIH kit. Evaluation studies showed no antisera contamination in both kits, but confirmed LH-contamination of the NIH hPRL VLS4 tracer. This may explain the reported wide prolactin variations.
Adejuwon
C A
CA
Center for Biomedical Research, Rockefeller University, New York, NY 10021.
D000293:Adolescent; D000328:Adult; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood*; D008598:Menstruation / Q000097:blood*; D011388:Prolactin / Q000097:blood*; D011863:Radioimmunoassay / Q000592:standards*; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012016:Reference Values; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic; D011388:Prolactin; D007986:Luteinizing Hormone