Isolation and characterization of a fatty acid binding protein in adipose tissue of Gallus domesticus.
1. Fatty acid binding protein (A-FABP) was isolated from chicken adipose cytosol. 2. Relative mol. wt of chicken A-FABP was determined to be 14,400 from SDS-polyacrylamide electrophoresis; the pI was 5.1; and amino acid composition data indicated structural homology with mammalian heart and adipose FABPs. 3. Polyclonal antisera prepared against A-FABP exhibited monospecificity for chicken A-FABP and no cross-reactivity with chicken liver proteins was observed. 4. Determination of relative ligand binding characteristics indicated A-FABP exhibited greatest binding activity in response to linoleate, followed by oleate, palmityl CoA and palmitate; no binding affinity for cholesterol was detected.
Department of Poultry Science, Texas A & M University System, College Station 77843-2472.
G H
GH
Sams
B M
BM
Hargis
P S
PS
Hargis
D000273:Adipose Tissue / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D002645:Chickens / Q000378:metabolism*; D002850:Chromatography, Gel; D003600:Cytosol / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D007106:Immune Sera / Q000276:immunology; D007526:Isoelectric Point; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008099:Liver / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010171:Palmitoyl Coenzyme A / Q000378:metabolism
D000596:Amino Acids; D002352:Carrier Proteins; D000939:Epitopes; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D007106:Immune Sera; D008041:Linoleic Acids; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D009829:Oleic Acids; D010169:Palmitic Acids; D010171:Palmitoyl Coenzyme A; D019301:Oleic Acid; D019308:Palmitic Acid; D019787:Linoleic Acid