Transforming growth factor beta 1 has differential effects on B cell proliferation and activation antigen expression.
There is accumulating evidence that TGF beta 1 is an important immunoregulatory molecule. Here we report evidence that TGF beta 1 has potent effects on murine B cells. It is profoundly inhibitory to the proliferation of quiescent B cells activated in model systems using both thymus-independent and thymus-dependent stimuli and arrests cells predominantly at the G1 cell-cycle stage. It also blocks the proliferation of B cell blasts, with a similar accumulation at stage G1. In parallel with this antiproliferative effect, TGF beta 1 inhibits induction of the expression of a series of "activation Ag" including transferrin receptor, RL388, and Ly-6, in mitogen-stimulated B cells. It also inhibits the induction of Ly-6 expression by IL-4, a nonmitogenic stimulus. In contrast to these negative influences, TGF beta 1 induces modestly increased expression of MHC class II Ag in quiescent B cells, and more marked increases in both B cell blasts and mitogen-stimulated cells. We speculate that in the appropriate context TGF beta 1 may be a cytokine that promotes productive B cell-Th cell interaction.
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000950:Antigens, Ly / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D003911:Dextrans / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D015847:Interleukin-4 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D011990:Receptors, Transferrin / Q000378:metabolism; D015291:Transforming Growth Factors / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D003911:Dextrans; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D011990:Receptors, Transferrin; D015847:Interleukin-4; D015291:Transforming Growth Factors