Vaccination against polyoma virus (PyV) tumors using vaccinia-PyV recombinants: a major tumor-specific transplantation antigen (TSTA) epitope resides within the C-terminal segment of middle-T protein.
We previously reported that inoculation of rats with live vaccinia virus (VV) recombinants VVpyMT, VVpyLT expressing either the middle-T (MT) or large-T (LT) proteins of polyomavirus (PyV) can elicit immunity to challenge with syngeneic PyV-tumor cells. We now report the results of cross-vaccination studies. VVpyMT was ineffective against cells expressing LT protein but prevented development of MT-expressing cells. Conversely, the VVpyLT was ineffective against MT-expressing cells. In the two experiments performed, tumor growth enhancement rather than retardation was observed in VVpyLT-vaccinated animals receiving PyV-LT (FRLTI) challenge tumor cells. To determine the location of the major TSTA within MT, a further VV recombinant (VVpyMT/Cfr) was constructed that expresses only the unique C-terminal segment of MT. VVpyMT-Cfr and VVpyMT were equally effective in eliciting tumor immunity, indicating the presence of a major TSTA epitope within the unique C-terminal region of MT.
LGME-CNRS & U184-INSERM, Strasbourg, France.
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Kieny
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Gautier
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Tomasetto
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Kuhn
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Hareuveni
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Lathe
D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D003429:Cross Reactions / Q000276:immunology; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D007114:Immunization; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000235:genetics / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D011994:Recombinant Proteins / Q000276:immunology; D014158:Transcription, Genetic / Q000235:genetics; D014412:Tumor Virus Infections / Q000276:immunology / Q000517:prevention & control*; D014616:Vaccinia virus / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000952:Antigens, Polyomavirus Transforming; D000939:Epitopes; D011994:Recombinant Proteins; D014765:Viral Vaccines