HLA-DR, DQ and DP antigen expression in synovial fluid T lymphocytes in rheumatoid arthritis: cell cycle analysis of HLA-DP positive T cells.
Cell surface expression of HLA-DR, DQ and DP antigens on synovial fluid (SF) T cells was analyzed in 43 patients with rheumatoid arthritis. Marked increase in the positive expression of all 3 Ia antigens was observed in the SF T cells compared to the counterpart, peripheral blood T cells. Both the DNA and RNA content of HLA-DP+ T cells were analyzed using acridine orange stain. RNA content in the HLA-DP+ SF T cells increased in comparison to that in the HLA-DP- SF T cells or normal peripheral blood T cells. Our findings suggest that majority of the SF T cells are activated and express HLA-DP antigen, while not proliferating.
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
T
T
Hirose
M
M
Goto
K
K
Okumura
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000503:physiopathology; D002453:Cell Cycle; D004247:DNA / Q000032:analysis; D005260:Female; D006680:HLA Antigens / Q000032:analysis*; D006682:HLA-DP Antigens / Q000032:analysis; D006683:HLA-DQ Antigens / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D012313:RNA / Q000032:analysis; D013582:Synovial Fluid / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000032:analysis / Q000166:cytology / Q000276:immunology*
D006680:HLA Antigens; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D012313:RNA; D004247:DNA