Simultaneous high-performance liquid chromatographic determination of norepinephrine, serotonin, acetylcholine and their metabolites in the cerebrospinal fluid of anaesthetized normotensive rats.
A high-performance liquid chromatographic method with electrochemical detection was developed for the simultaneous determination of the levels of norepinephrine (NE), serotonin (5-HT), acetylcholine (ACh) and their metabolites in the cerebrospinal fluid (CSF) of anaesthetized rats. The response curve for each compound was linear for the concentration way of interest. The within- and between-day coefficients of variation (C.V.) for NE, 5-HT and their metabolites were less than 7.85% and 15.67%, respectively, and those for ACh and choline were less than 3.08% and 6.27%, respectively. This simultaneous determination should be useful for elucidating the noradrenergic, serotonergic and cholinergic nerve activity in the central nervous system.
Matsumoto
M
M
First Department of Pharmacology, Hokkaido University School of Medicine, Sapporo, Japan.
Togashi
H
H
Yoshioka
M
M
Hirokami
M
M
Morii
K
K
Saito
H
H
D015102:3,4-Dihydroxyphenylacetic Acid / Q000134:cerebrospinal fluid; D000109:Acetylcholine / Q000134:cerebrospinal fluid* / Q000378:metabolism; D000768:Anesthesia, General; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid; D007274:Injections, Intraperitoneal; D008297:Male; D009638:Norepinephrine / Q000134:cerebrospinal fluid* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000134:cerebrospinal fluid* / Q000378:metabolism
D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D000109:Acetylcholine; D009638:Norepinephrine; D006719:Homovanillic Acid