Characterization of highly virulent Escherichia coli strains by ribosomal DNA restriction fragment length polymorphism.
Patterns of ribosomal DNA polymorphism were examined to compare carboxylesterase B type B1 strains and B2 strains of Escherichia coli isolated from extra-intestinal infections. DNA from 14 type B2 strains showing the presence of alpha-haemolysin and mannose-resistant haemagglutinin and lethality to mice and 14 type B1 strains lacking these characteristics, was digested with HindIII, EcoRI, BamHI or BglII restriction enzymes and analysed by Southern blotting. The obtained ribotypes clearly differentiated the B2 strains from the B1 strains. These results indicate that genotypes of the highly virulent B2 strains are different from that of the less virulent B1 strains.
Picard
B
B
Laboratoire de Microbiologie, Faculté de Médecine Xavier Bichat, Université Paris, France.
Picard-Pasquier
N
N
Krishnamoorthy
R
R
Goullet
P
P
D015139:Blotting, Southern; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D005602:France; D006388:Hemagglutinins / Q000032:analysis; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D018805:Sepsis / Q000382:microbiology; D014552:Urinary Tract Infections / Q000382:microbiology; D014774:Virulence / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006388:Hemagglutinins; D006460:Hemolysin Proteins; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase