Expression in Escherichia coli of a dominant immunogen of Trypanosoma cruzi recognized by human chagasic sera.
A genomic clone expressing a Trypanosoma cruzi antigen in Escherichia coli was identified using human chagasic sera. Chagasic antibodies affinity purified on extracts of this clone recognized a high-molecular-weight protein expressed in all developmental stages of the parasite life cycle, as well as in various T. cruzi strains. The antigen is associated with the cytoskeleton of the parasite and localizes along the attachment region between the flagellum and the cell body. Antibodies to the recombinant antigen were detected in the sera of 115 chagasic patients from different endemic regions, but not in sera of patients with leishmaniasis, T. rangeli infection, or other parasitic diseases. Our data suggest that the presence of antibodies to this antigen may be specifically associated with Chagas' disease.
Disciplina de Parasitologia, Escola Paulista de Medicina, São Paulo, Brazil.
P C
PC
Cotrim
G S
GS
Paranhos
R A
RA
Mortara
J
J
Wanderley
A
A
Rassi
M E
ME
Camargo
J F
JF
da Silveira
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D014355:Chagas Disease / Q000276:immunology*; D003001:Cloning, Molecular; D003599:Cytoskeleton / Q000276:immunology; D004247:DNA / Q000235:genetics; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D014349:Trypanosoma cruzi / Q000235:genetics / Q000276:immunology* / Q000648:ultrastructure
D000953:Antigens, Protozoan; D000939:Epitopes; D007106:Immune Sera; D004247:DNA