Lack of involvement of the G-CSF gene by chromosomal translocation t(15;17) in acute promyelocytic leukemia.
Using a human G-CSF cDNA as a probe, we analyzed the t(15;17) breakpoint by Southern blot analysis with conventional and/or pulsed-field gel electrophoresis in 12 patients with acute promyelocytic leukemia. The results did not show the rearrangement, deletion, or restriction fragment length polymorphism within the gene and in the surrounding sequences.
Third Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
S
S
Tanaka
H
H
Nishigaki
S
S
Misawa
M
M
Taniwaki
H
H
Nakagawa
H
H
Yashige
S
S
Horiike
K
K
Kashima
J
J
Inazawa
Y
Y
Sonoda
D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15 / Q000502:physiology; D002886:Chromosomes, Human, Pair 17 / Q000502:physiology; D003115:Colony-Stimulating Factors / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009857:Oncogenes / Q000502:physiology; D014178:Translocation, Genetic / Q000235:genetics*
D003115:Colony-Stimulating Factors; D016179:Granulocyte Colony-Stimulating Factor; D004262:DNA Restriction Enzymes