Binding of enteric bacteria to hog gastric mucin.
The binding features of enteric bacteria were studied using a model mucin of hog gastric origin. The time requirement of binding is short, it is temperature-independent, but dose-dependent. The binding effectiveness of Escherichia coli, Shigella sonnei and Shigella flexneri, as well as Salmonella minnesota had a narrow range: 1.5-9 germs pro pg of mucin. The bacterial ligand of the binding is certainly not a polysaccharide as proved by the uniform binding of the R-mutant series of S. sonnei and S. minnesota. On the basis of inhibition tests by an outer membrane protein fraction, the ligand may be a common outer membrane protein of the enteric bacteria. The outer membrane proteins encoded by the Shigella-EIEC invasivity plasmids do not take part in this binding. The inhibition by killed bacteria or by their culture supernatants of mucin binding of heterologous species may suggest a non-species specific common ligand, too. Similarly to the mucin utilization, the binding ability also seems to be a general phenomenon among the enteric bacteria.
Institute of Microbiology, University Medical School, Pécs, Hungary.
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D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D004755:Enterobacteriaceae / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology; D005752:Gastric Mucins / Q000502:physiology*; D008070:Lipopolysaccharides / Q000502:physiology; D012475:Salmonella / Q000502:physiology; D012763:Shigella flexneri / Q000502:physiology; D012764:Shigella sonnei / Q000502:physiology; D013552:Swine
D001425:Bacterial Outer Membrane Proteins; D005752:Gastric Mucins; D008070:Lipopolysaccharides