[Antinuclear, anticentromere and anti-ScL-70 antibodies in rheumatic diseases].
Methods are described that are used for the titration of antinuclear, anticentromere, and anti-Scl-70 antibodies in systemic scleroderma, systemic lupus erythematosus, and rheumatoid arthritis: indirect immunofluorescence with various antigenic substrates (sections of fresh-frozen rat liver and Hep-2 cell culture), counter-current immunoelectrophoresis, isolation of Scl-70 antigen. Use of Hep-2 cells as a substrate for indirect immunofluorescence was found clinically and diagnostically more effective since it permitted the detection of anticentromere antibodies and anti-Scl-70. Nucleolar, mottled, homogeneous, marginal immunofluorescence types were observed when rat liver sections and Hep-2 cells were used for substrates. Anticentromere antibodies and anti-Scl-70 were isolated significantly more frequently in systemic lupus erythematosus or rheumatoid arthritis.
Ivanova
S M
SM
Melkumova
K L
KL
Il'in
K V
KV
Riazantseva
T A
TA
Piven'
V A
VA
Speranskiĭ
A I
AI
D000974:Antibodies, Antinuclear / Q000032:analysis*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis*; D002503:Centromere / Q000276:immunology*; D004264:DNA Topoisomerases, Type I; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009687:Nuclear Proteins / Q000276:immunology*; D012595:Scleroderma, Systemic / Q000276:immunology*
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D009687:Nuclear Proteins; C048100:Scl 70 antigen, human; D004264:DNA Topoisomerases, Type I
Antinuklearnye, antitsentromernye, anti-ScL-70 antitela pri revmaticheskikh zabolevaniiakh.