Time dependent uptake of (-)125I-iodocyanopindolol but not (-)125I-iodopindolol by murine splenic lymphocytes.
Time dependent uptake of (-)125I-iodocyanopindolol by intact murine splenocytes was investigated in an attempt to prevent its interference in receptor binding experiments. Four approaches were tried in an attempt to eliminate this uptake; including the use of the permeable amine chloroquine; the inclusion of catechol, chloroquine, and phentolamine into the binding assay; the addition of methylamine to the assay; and the elimination of ascorbate from the assay. None of these approaches completely eliminated the uptake of ICYP over time. Comparative studies with (-)125I-iodopindolol showed this ligand did not have time dependent uptake and may provide for less problematic intact cell receptor assays.
Johnson
C C
CC
Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0613.
Fuchs
B A
BA
D000319:Adrenergic beta-Antagonists / Q000378:metabolism*; D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D010869:Pindolol / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D013154:Spleen / Q000378:metabolism
D000319:Adrenergic beta-Antagonists; D007457:Iodine Radioisotopes; C029247:3-iodopindolol; D020120:Iodocyanopindolol; D010869:Pindolol