Bone marrow processing with a gravity sedimentation technique: experience of 13 cases.
The experience of 13 bone marrow processings is reported: 7 patients were affected by Acute Leukemias, 4 by Non Hodgkin's Lymphomas, 1 by Chronic Myelogenous Leukemia in complete remission after induction chemotherapy, 1 by Ewing's Sarcoma. Gravity sedimentation technique with 6% hydroxyethyl starch was used in all cases. A mean value of 0.754 x 10(8)/kg body weight mononuclear cells was harvested after sedimentation, with a mean recovery of 74.554%. Mean CFU-GM value was 0.543 x 10(4)/kg body weight. Only one evaluable patient, affected by ANLL, underwent Autologous Bone Marrow Transplantation and a full engraftment was noted on day +14. Results are discussed and further studies are proposed to clarify the relationship between the in vitro CFU-GM growth and the bone marrow engraftment.
Adorno
G
G
Cattedra di Ematologia, II Università degli Studi di Roma, Italy.
Tribalto
M
M
Cantonetti
M
M
di Toritto
T C
TC
Cudillo
L
L
Dentamaro
T
T
Iampieri
M P
MP
Lucanto
E
E
Masi
M
M
Riguzzi
M C
MC
D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000379:methods*; D005260:Female; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D013048:Specimen Handling / Q000379:methods*
D006895:Hydroxyethyl Starch Derivatives