Amino acid side chain interaction with chelate-liganded crosslinked dextran, agarose and TSK gel. A mini review of recent work.
Protein adsorption and retention data collected from recent chromatographic studies on hydrophilic gels substituted with chelate-bonded metal ions are discussed. Attempts are made to interpret the adsorption behavior in terms of molecular events caused by the affinity for the immobilized metal ions.
Porath
J
J
University of Arizona, Division of Biotechnology, Tucson 85721.
D000327:Adsorption; D000596:Amino Acids / Q000302:isolation & purification*; D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods*; D003911:Dextrans; D005782:Gels; D006493:Heparin; D007477:Ions; D008670:Metals; D011108:Polymers; D011506:Proteins / Q000302:isolation & purification; D012685:Sepharose
D000596:Amino Acids; D002614:Chelating Agents; D003911:Dextrans; D005782:Gels; D007477:Ions; D008670:Metals; D011108:Polymers; D011506:Proteins; C058416:TSKgel heparin-5PW; D006493:Heparin; D012685:Sepharose