Response to fibronectin of mouse primordial germ cells before, during and after migration.
The adhesive extracellular matrix glycoprotein fibronectin is thought to play a central role in cell migration during embryogenesis. In order to define this role, we have examined the response to fibronectin in cell culture of mouse primordial germ cells (PGCs) before, during and after their migration from the hindgut into their target tissue, the genital ridges. Using an explant culture system, we show that PGCs will emigrate from tissue fragments containing hindgut, and that fibronectin stimulates this migration. Adhesion assays show that the start of PGC migration is associated with a fall in adhesion to fibronectin. Double-labelling studies using in situ hybridization and histochemistry demonstrate that migrating PGCs do not contain detectable fibronectin mRNA, suggesting that they do not synthesize and secrete the fibronectin within their migratory substratum. Taken together, these findings are consistent with an important role for fibronectin in stimulating PGC migration. In addition, however, they suggest that the interaction between PGCs and fibronectin may be important in timing the start of migration, with the fall in adhesion allowing the PGCs to commence their migration towards the genital ridges.
Ffrench-Constant
C
C
Department of Zoology, Cambridge University, UK.
Hollingsworth
A
A
Heasman
J
J
Wylie
C C
CC
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D005109:Extracellular Matrix; D005353:Fibronectins / Q000494:pharmacology* / Q000502:physiology; D005854:Germ Cells / Q000187:drug effects* / Q000648:ultrastructure; D006651:Histocytochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009024:Morphogenesis / Q000187:drug effects*; D013268:Stimulation, Chemical
D005353:Fibronectins