A simple method for improved visualization of the lamellated structure of cutinized and suberized plant cell walls by electron microscopy.
By treating ultrathin sections with H2O2 prior to normal uranyl acetate and lead citrate staining, a strong increase in contrast of cutinized and suberized plant cell walls can be achieved.
Heumann
H G
HG
Botanical Institute I, University of Karlsruhe, Federal Republic of Germany.
D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D002951:Citrates; D019343:Citric Acid; D006861:Hydrogen Peroxide; D008055:Lipids; D008563:Membrane Lipids / Q000378:metabolism; D008854:Microscopy, Electron; D009942:Organometallic Compounds; D010944:Plants / Q000378:metabolism / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods*
D002951:Citrates; D008055:Lipids; D008563:Membrane Lipids; D009942:Organometallic Compounds; C005460:uranyl acetate; D019343:Citric Acid; C000521:cutin; C065875:suberin; D006861:Hydrogen Peroxide