Nucleotide sequence of the Yersinia pestis gene encoding F1 antigen and the primary structure of the protein. Putative T and B cell epitopes.
The plasmid-located gene caf1 encoding the capsular antigen fraction 1 (F1) of Yersinia pestis was cloned and sequenced. The gene codes for a 170 amino acid peptide with a deduced Mr of 17.6 kDa. The signal peptide sequence was highly homologous to the E. coli consensus signal sequence. The F1 was assumed to have beta-sheet structure for the most part. The region located between amino acids 100 and 150 was suggested to contain putative antigenic determinants and to stimulate T cells.
Institute of Immunology, USSR Ministry of Medical Industry, Lyubuchany, Moscow Region.
E E
EE
Galyov
Smirnov OYu
A V
AV
Karlishev
K I
KI
Volkovoy
A I
AI
Denesyuk
I V
IV
Nazimov
K S
KS
Rubtsov
V M
VM
Abramov
S M
SM
Dalvadyanz
V P
VP
Zav'yalov
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D000939:Epitopes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D015394:Molecular Structure; D015183:Restriction Mapping; D015010:Yersinia pestis / Q000235:genetics*
D000942:Antigens, Bacterial; D000939:Epitopes