[Progress in the development of a detection test for parenteral non-A, non-B hepatitis--results of enzyme immunoassay for anti-hepatitis C virus].
After more than one decennium of international research work the doubtless identification of the causative agents of the non A-non B-hepatitis (NANBH) has not yet been successful. 1988, however, a viral genome of the parenteral NANBH could be isolated, on which basis an EIA was built up. By means of this anti-HCV-ELISA altogether 413 sera were tested. In 262 sera of 154 women of a NANBH-group with homogeneous source of infection (contaminated anti-D-immunoglobulin) in 74% positive reactions were the result. This and the extensive reproducibility of the test results in identical patients speak for the fact that the recombinant antigen underlying the test really belongs to the parenteral NANBH-group. In the group of the sporadic, however, only in one case a positive reaction was achieved, which supports the thesis of at least two parenteral causative agents of NANBH. The deep-freezing storage of patients' sera lasting up to 8 years did not lead to the failure of the test. The reasons for non-reactive tests were discussed.
Klinik für Infektions- und Tropenkrankheiten, Bezirkskrankenhauses St. Georg Leipzig.
M
M
Wiese
K
K
Stolle
M
M
Ehrenberg
D000328:Adult; D000956:Antigens, Viral / Q000276:immunology*; D001782:Blood Donors; D001793:Blood Preservation; D001803:Blood Transfusion; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006508:Hepatitis Antibodies / Q000032:analysis*; D006526:Hepatitis C / Q000175:diagnosis* / Q000276:immunology / Q000635:transmission; D018936:Hepatitis C Antigens; D006525:Hepatitis, Viral, Human / Q000175:diagnosis*; D006801:Humans; D012307:Risk Factors
D000956:Antigens, Viral; D006508:Hepatitis Antibodies; D018936:Hepatitis C Antigens
Fortschritte bei der Entwicklung eines Nachweistestes der parenteralen Non A-Non B-Hepatitis--Ergebnisse mit dem Enzymimmunassay Anti-HCV (Hepatitis-C-Virus).