LFA-3, CD44, and CD45: physiologic triggers of human monocyte TNF and IL-1 release.
The monocyte-derived cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta), are central regulators of the immune response, but the physiologic stimuli for their release remain largely undefined. Engagement of three monocyte glycoproteins, LFA-3, CD44, and CD45, by specific monoclonal antibodies immobilized on plastic induced TNF-alpha and IL-1 beta release. In addition, TNF-alpha was released when monocyte LFA-3 bound immobilized, purified CD2, which is its physiologic receptor. Thus, a receptor-ligand interaction that mediates cell-cell adhesion can transmit the necessary signals for the release of monokines.
Webb
D S
DS
Food and Drug Administration, Division of Cytokine Biology, Bethesda, MD 20892.
Shimizu
Y
Y
Van Seventer
G A
GA
Shaw
S
S
Gerrard
T L
TL
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000502:physiology*; D000954:Antigens, Surface / Q000502:physiology*; D018968:CD58 Antigens; D006649:Histocompatibility Antigens / Q000502:physiology*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D017493:Leukocyte Common Antigens; D008070:Lipopolysaccharides / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000502:physiology*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D015846:Monokines / Q000378:metabolism*; D016201:Receptors, Lymphocyte Homing; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D018968:CD58 Antigens; D006649:Histocompatibility Antigens; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D015846:Monokines; D016201:Receptors, Lymphocyte Homing; D014409:Tumor Necrosis Factor-alpha; D017493:Leukocyte Common Antigens