N-linked oligosaccharides during human renal organogenesis.
The structure of fucosylated derivatives of N-acetyl lactosamine expressed during human renal development has been studied by the combined use of monoclonal antibodies and glycosyl hydrolases. LeX is expressed on the ureteric bud ampulla carried N-linked on a glycoprotein. It is also present in the proximal tubule as a ganglioside. H-Type II substance is expressed on the endothelium of the developing renal vasculature. It is also carried N-linked to a glycoprotein. These regulated changes in the cell surface carbohydrate expression are important in understanding cell interactions and receptor systems during renal development.
University Department of Pathology, Southampton General Hospital.
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Fleming
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D000939:Epitopes / Q000032:analysis; D005333:Fetus / Q000276:immunology; D006241:Haptens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007519:Isoantigens / Q000032:analysis; D007668:Kidney / Q000196:embryology* / Q000276:immunology; D009844:Oligosaccharides / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D006241:Haptens; D007519:Isoantigens; D009844:Oligosaccharides