Differential binding of serum glycoproteins to lectins during hepatic regeneration in hepatocellular carcinoma and fulminant hepatic failure.
1. The concentrations of four serum glycoproteins, thyroxine-binding globulin, alpha 2-macroglobulin, alpha 1-antitrypsin and transferrin, as well as their reactivities with concanavalin A and lentil-lectin, were measured in patients with hepatocellular carcinoma or fulminant hepatic failure and in normal subjects. 2. Serum concentrations of thyroxine-binding globulin and alpha 1-antitrypsin were significantly greater in patients with hepatocellular carcinoma than in normal subjects, and the percentage lentil-lectin reactivity of these two proteins was markedly increased. 3. With the exception of transferrin, which did not bind to lentil-lectin, an enhancement of lentil-lectin reactivity was observed for the glycoproteins in serum from patients with fulminant hepatic failure. No difference in concanavalin A binding was found between the groups for any of the glycoproteins. 4. Altered fucosylation, as indicated by increased lentil-lectin binding, occurs in several glycoproteins arising in malignant and non-malignant conditions associated with abnormal hepatic regeneration.
Liver Unit, King's College Hospital, London.
M Q
MQ
Du
W L
WL
Hutchinson
P J
PJ
Johnson
R
R
Williams
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000097:blood; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000378:metabolism; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008107:Liver Diseases / Q000097:blood*; D008113:Liver Neoplasms / Q000097:blood; D008115:Liver Regeneration; D008875:Middle Aged; D037121:Plant Lectins; D013975:Thyroxine-Binding Proteins / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism
D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; D013975:Thyroxine-Binding Proteins; D014168:Transferrin; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; C033619:lentil lectin; D003208:Concanavalin A