Simultaneous high-performance liquid chromatographic determination of norepinephrine, serotonin, acetylcholine and their metabolites in the cerebrospinal fluid of anaesthetized normotensive rats.
A high-performance liquid chromatographic method with electrochemical detection was developed for the simultaneous determination of the levels of norepinephrine (NE), serotonin (5-HT), acetylcholine (ACh) and their metabolites in the cerebrospinal fluid (CSF) of anaesthetized rats. The response curve for each compound was linear for the concentration way of interest. The within- and between-day coefficients of variation (C.V.) for NE, 5-HT and their metabolites were less than 7.85% and 15.67%, respectively, and those for ACh and choline were less than 3.08% and 6.27%, respectively. This simultaneous determination should be useful for elucidating the noradrenergic, serotonergic and cholinergic nerve activity in the central nervous system.
First Department of Pharmacology, Hokkaido University School of Medicine, Sapporo, Japan.
M
M
Matsumoto
H
H
Togashi
M
M
Yoshioka
M
M
Hirokami
K
K
Morii
H
H
Saito
D015102:3,4-Dihydroxyphenylacetic Acid / Q000134:cerebrospinal fluid; D000109:Acetylcholine / Q000134:cerebrospinal fluid* / Q000378:metabolism; D000768:Anesthesia, General; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid; D007274:Injections, Intraperitoneal; D008297:Male; D009638:Norepinephrine / Q000134:cerebrospinal fluid* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000134:cerebrospinal fluid* / Q000378:metabolism
D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D000109:Acetylcholine; D009638:Norepinephrine; D006719:Homovanillic Acid