Low-cost image processing system for analyzing molecular RNA fingerprints.
A complete image digitizing and processing system is described for capturing, enhancing and analyzing molecular fingerprints. The low-cost, high-resolution system features a Motorola 68000 processor, multi-tasking, a separate video coprocessor, and color or gray scale processing. Thousands of manipulations are possible using functions which include histographic equalization, edge detection, filtering, overlays, false coloring, zoom, pan and print. All operations are initiated and controlled with a mouse. Techniques for enhancing, scaling and comparing molecular fingerprints are described. The techniques all involve using a graphical interface to select and manipulate the various processes. The system has been used successfully for about 1.5 years, and it has been ideal for our application which requires human judgment at many steps between processing and which probably would not lend itself to a completely automated analysis. Similar techniques could probably be used with this system on many other applications.
Mitchell
B W
BW
U.S. Department of Agriculture, Southern Poultry Research Laboratory, Athens, CA 30604.
Palmieri
S
S
D003365:Costs and Cost Analysis; D007091:Image Processing, Computer-Assisted; D009710:Nucleotide Mapping / Q000379:methods*; D012313:RNA / Q000032:analysis*
D012313:RNA