Polymorphism of apolipoprotein A-II (apoA-II) among inbred strains of mice. Relationship between the molecular type of apoA-II and mouse senile amyloidosis.
Three types of apolipoprotein A-II (apoA-II) proteins (A, B and C) were predicted from the nucleotide sequence of apoA-II cDNA. Substitution of amino acid residues was noted at four positions (type A: Pro-5, Asp-20, Met-26, Ala-38; B: Pro-5, Glu-20, Val-26, Val-38; C: Gln-5, Glu-20, Val-26, Ala-38). Each type was identifiable by digestion of amplified apoA-II DNA by PCR, using restriction-fragment-length polymorphism of the apoA-II gene for restriction enzymes Cfr13I and MspI. The molecular type of apoA-II was determined among 23 strains of mice including nine of the senescence accelerated mouse series developed in our laboratory. Examination of types of apoA-II and amyloid deposition in the F2 and F3 hybrid mice showed that apoA-II amyloid deposition was present only in the mice homozygous for type C apoA-II and which were 12-17 months of age. The molecular type of apoA-II may be a factor involved in the development of senile amyloidosis in mice.
Higuchi
K
K
Department of Senescence Biology, Kyoto University, Japan.
Kitagawa
K
K
Naiki
H
H
Hanada
K
K
Hosokawa
M
M
Takeda
T
T
D000375:Aging; D000595:Amino Acid Sequence; D000686:Amyloidosis / Q000235:genetics*; D000818:Animals; D016633:Apolipoprotein A-II / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D006824:Hybridization, Genetic; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length
D016633:Apolipoprotein A-II; D004247:DNA