Comparative effects of vecuronium and pancuronium on release of lactate dehydrogenase in rat isolated diaphragm, liver, kidney and heart.
The comparative effects of two muscle relaxants, namely pancuronium and vecuronium, on the release of intracellular lactate dehydrogenase (LDH), released from four different types of tissues, namely, the heart, liver, kidney and diaphragm, were studied in the rat in vitro. The LDH and its leakage into extracellular space, was used as an indicator for cellular membrane damage, and the aim was to see if the two muscle relaxants enhanced the release of LDH, and hence, by implication, caused adverse effects, i.e., damage to cell membrane. The total amount of LDH, the neat (LDH), and the increase in LDH release caused by different concentrations of the two muscle relaxants were measured using spectrophotometric determination at 340 nm. The results showed that both muscle relaxants, in low concentrations (0.32 or 0.34 microM, a concentration which is close to a clinical dose of 0.1 mg/ml), did not significantly enhance the LDH release from any of the 4 different types of tissues studied. However, in concentrations of 10-130 times the clinical dose, both drugs significantly enhanced the LDH release in all the 4 tissues, with pancuronium being more effective than vecuronium. In addition, the diaphragm and kidney were most affected, whereas the heart and liver being the least affected. The clinical relevance and/or implications of these comparative effects are discussed, and the results are compared to those previously reported by other workers. Pancuronium and vecuronium are among the non-depolarising neuromuscular blocking drugs currently-used in clinical anaesthesia.(ABSTRACT TRUNCATED AT 250 WORDS)
Respiratory and Anaesthetics Unit, Hospital for Sick Children, London, England.
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Wali
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Makinde
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D003964:Diaphragm / Q000187:drug effects / Q000201:enzymology; D006321:Heart / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D010197:Pancuronium / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014673:Vecuronium Bromide / Q000494:pharmacology*
D014673:Vecuronium Bromide; D007770:L-Lactate Dehydrogenase; D010197:Pancuronium