pH-control of the miscibility properties of a binary lipid alloy and its influence on the ion transport by gramicidin.
We studied the coupling of a membrane function (the transport of ions by the pore forming polypeptide gramicidin) to chemically driven phase changes in black membranes of binary lipid mixtures. In particular, we investigated the influence of the aqueous pH value on the fluid-fluid demixing effect of Ca2+ to phosphatidylcholine/phosphatidylglycerol bilayers. It is found that one can switch, under certain conditions, between a homogeneously mixed and a phase separated membrane by changing the pH. We interpret this as being caused by the change in the degree of dissociation of one of the lipid components.
Mittler-Neher
S
S
Max-Planck-Institut für Polymerforschung, Mainz, F.R.G.
Knoll
W
W
D004553:Electric Conductivity; D006096:Gramicidin; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D012995:Solubility
D007473:Ion Channels; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D006096:Gramicidin