[Monoclonal antibodies IGR to nuclear antigens of neutrophilic granulocytes of human blood].
Four monoclonal antibodies (Mabs) of series IGR-1 and IGR-2 to nuclear antigens of neutrophilic granulocytes of human peripheral blood were obtained. Mabs IGR-1 2B8 and IGR-1 6B5 are bound to their specific antigens in the nuclei of all the investigated human cell lines. These Mabs were also specific for metaphase chromosomes of cell lines HL-60 and U-937. Investigations on the ultrastructural level showed that Mabs IGR-1 6B5 reacted with the HL-60 nuclear heterochromatin region. Mabs IGR-1 3D3 and IGR-2 2F1 manifested high specificity only for the nuclei of mature neutrophils and of plasma cells.
Ingerpuu
S O
SO
Piĭrsoo
A O
AO
Siunter
A E
AE
Kivi
S Iu
SIu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000943:Antigens, Differentiation / Q000276:immunology*; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D000939:Epitopes; D005453:Fluorescence; D006570:Heterochromatin / Q000032:analysis; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000276:immunology; D008677:Metaphase; D051379:Mice; D009101:Multiple Myeloma / Q000235:genetics / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D010950:Plasma Cells / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000939:Epitopes; D006570:Heterochromatin
Monoklonal'nye antitela IGR k iadernym antigenam neĭtrofil'nykh granulotsitov krovi cheloveka.