Expression of rat prolactin-like protein B in deciduoma of pseudopregnant rat and in decidua during early pregnancy.
The rat PRL-like protein-B (rPLP-B) complementary DNA (cDNA), originally cloned from a late term placental library, hybridizes to transcripts in the deciduoma tissue artificially produced in pseudopregnant rats. The expression of rPLP-B in deciduoma is first observed 48 h (pseudopregnancy day 7) after the deciduogenic stimulus and increases to a maximum by 96-120 h (pseudopregnancy day 9-10). In situ hybridization studies show that rPLP-B hybridizes specifically to the antimesometrial cells of deciduoma tissue in day 9 pseudopregnant rats and to the homologous cells of the decidua that surround the embryo-trophoblastic structure at day 9 of pregnancy. A temporal study on the expression of rPLP-B during pregnancy shows that rPLP-B is concomitantly expressed by the decidual cells of maternal origin and the cytotrophoblast of fetal origin around day 13 of pregnancy.
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
F
F
Croze
T G
TG
Kennedy
I C
IC
Schroedter
H G
HG
Friesen
D000818:Animals; D015342:DNA Probes; D003656:Decidua / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005333:Fetus / Q000378:metabolism; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010052:Ovariectomy; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood / Q000235:genetics*; D011270:Pregnancy, Animal / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D011555:Pseudopregnancy / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic / Q000187:drug effects
D015342:DNA Probes; D011257:Pregnancy Proteins; D012333:RNA, Messenger; C066551:prolactin-like protein B; D011374:Progesterone; D004958:Estradiol; D012313:RNA