Analysis of the structure and variability of nucleolar organizer regions of Salmo trutta by C-, Ag-, and restriction endonuclease banding.
Nucleolar organizer regions (NORs) of brown trout were investigated using C-, Ag-, and restriction endonuclease banding. The presence of constitutive heterochromatin was confirmed by C-banding. Giemsa-staining, C-banding, and Ag-banding revealed great variability in the size of the short arm of the NOR-bearing chromosome. This size variation was due in some cases to NOR duplication. Restriction endonuclease digestion induced a specific banding pattern for AluI, DdeI, HaeIII, MboI, and HinfI, indicating some features about the sequence composition of the NOR-associated heterochromatin.
Departamento de Biología Fundamental, Universidad de Santiago, Lugo, Spain.
L
L
Sánchez
P
P
Martínez
A
A
Viñas
C
C
Bouza
D000818:Animals; D002871:Chromosome Banding; D004262:DNA Restriction Enzymes / Q000378:metabolism; D006570:Heterochromatin / Q000737:chemistry* / Q000648:ultrastructure; D007621:Karyotyping; D009697:Nucleolus Organizer Region / Q000737:chemistry* / Q000648:ultrastructure; D012834:Silver; D013194:Staining and Labeling; D014337:Trout / Q000235:genetics*
D006570:Heterochromatin; D012834:Silver; D004262:DNA Restriction Enzymes