Histamine-evoked amylase secretion is associated with small changes in calcium mobilization in isolated guinea-pig pancreas.
An investigation was made of the effect of histamine on amylase secretion and calcium mobilization in isolated guinea-pig pancreatic segments. The effect of acetylcholine (ACh) was also examined for comparison. Histamine evoked a dose-dependent increase in amylase output from superfused segments. It had small transient effects on both 45Ca influx and efflux, and elevated cytosolic free Ca2+ concentration in acini. The equivalent dose of ACh evoked a greater amylase output and greater changes in Ca2+ mobilization. The results suggest that histamine may have a physiological role in exocrine secretion of the guinea-pig pancreas but is less potent than ACh.
School of Applied Biology, Lancashire Polytechnic, Preston, Spain.
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G
Salido
R
R
Lennard
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J
Singh
J A
JA
Pariente
D000109:Acetylcholine / Q000494:pharmacology / Q000502:physiology; D000681:Amylases / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology / Q000502:physiology*; D066298:In Vitro Techniques; D010179:Pancreas / Q000378:metabolism*
D006632:Histamine; D000681:Amylases; D000109:Acetylcholine; D002118:Calcium