[The effect of the binding of the antigenic determinant of tetrodotoxin-sensitive protein on the phytohemagglutinin-induced proliferative response of the mononuclear cells in mouse spleen].
Mammalian brain-derived "tetrodotoxin-sensitive protein" has been shown to share an epitope with as yet unidentified structure of the human and rodent lymphocyte surface. Previously obtained observations that a monoclonal antibody to this epitope induces a proliferative response of murine splenic mononuclear cells are confirmed. However, this antibody fails to modify the phytohemagglutinin-induced response. Moreover, lectin with submitogenic concentration inhibited the antibody-induced response provided that monocytes were present in the culture. The antibody-induced proliferation appeared to be less monocyte-dependent than the lectin-induced one. Taken together, these findings argue against hypothesis that a lymphocyte structure with epitope of the "tetrodotoxin-sensitive protein" is associated with either T cell receptor for antigen or interleukin-2 receptor.
Pinchuk
G V
GV
Pinchuk
L N
LN
D000818:Animals; D002352:Carrier Proteins / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000939:Epitopes / Q000378:metabolism* / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D015222:Sodium Channels; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013779:Tetrodotoxin / Q000276:immunology*
D002352:Carrier Proteins; D000939:Epitopes; D009419:Nerve Tissue Proteins; D010835:Phytohemagglutinins; D015222:Sodium Channels; C023790:tetrodotoxin-binding protein; D013779:Tetrodotoxin
Izuchenie vliianie sviazyvaniia antigennoĭ determinanty tetrodotoksinchuvstvitel'nogo belka na proliferativnyĭ otvet mononuklearnykh kletok selezenki myshi, indutsiruemyĭ fitogemaggliutininom.