Linkage mapping of highly informative DNA polymorphisms within the human interferon-alpha receptor gene on chromosome 21.
Two polymorphic loci within the interferon-alpha receptor (IFNAR) gene on human chromosome 21 have been identified and mapped by linkage analysis in 40 CEPH families. These markers are (1) a multiallelic RFLP with an observed heterozygosity of 0.72 and (2) a variable (AT3)n short sequence repeat at the poly(A) tail of an Alu sequence (AluVpA) with an observed heterozygosity of 0.83. This locus is close to D21S58 (theta = 0.02, zeta = 36.76) and D21S17 (theta = 0.02, Zeta = 21.76) with chromosomal band 21q22.1. Multipoint linkage analysis suggests the most likely locus order to be 21cen-D21S58-IFNAR-D21S17-21qter. Given its high heterozygosity, the IFNAR gene can be used as an index marker on human chromosome 21.
Center for Medical Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
M G
MG
McInnis
G
G
Lutfalla
S
S
Slaugenhaupt
M B
MB
Petersen
G
G
Uze
A
A
Chakravarti
S E
SE
Antonarakis
D001483:Base Sequence; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D005260:Female; D008040:Genetic Linkage / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism*; D007438:Introns / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D011971:Receptors, Immunologic / Q000235:genetics*; D017471:Receptors, Interferon; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D005819:Genetic Markers; D016898:Interferon-alpha; D011971:Receptors, Immunologic; D017471:Receptors, Interferon