Kinetochore formation in experimentally undercondensed chromosomes.
Treatment of human and mouse cell cultures with the cytidine analogue 5-azadeoxycytidine and the AT-specific DNA ligand Hoechst 33258 dramatically inhibited condensation of the pericentromeric heterochromatin in several chromosomes. When stained with antikinetochore autoimmune sera, these experimentally undercondensed chromosomes showed kinetochores with preserved antigenicity. The undercondensed and normally condensed chromosomes share the major antigenic determinants of the kinetochore.
Institut für Humangenetik der Universität, Würzburg, Federal Republic of Germany.
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D000818:Animals; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001562:Benzimidazoles / Q000494:pharmacology*; D006690:Bisbenzimidazole / Q000494:pharmacology*; D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure*; D000077209:Decitabine; D000939:Epitopes; D006570:Heterochromatin / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice
D001562:Benzimidazoles; D000939:Epitopes; D006570:Heterochromatin; D000077209:Decitabine; D006690:Bisbenzimidazole; D001374:Azacitidine