Determination of subgroup, serotype and electropherotype of human rotaviruses obtained from diarrheic patients in the Philippines.
A total of 40 rotavirus specimens obtained from infants with acute gastroenteritis in Metro Manila, Philippines, were analyzed for their subgroup and serotype specificities and RNA electropherotype. A half (50%) of the specimens contained subgroup I human rotaviruses (HRVs), while 45% of them contained subgroup II HRVs. All subgroup II HRVs were determined to be serotype 1, except one specimen belonging to serotype 3, by an enzyme-linked immunosorbent assay with serotype specific monoclonal antibodies. However, serotype specificity of all subgroup I specimens failed to be determined. In polyacrylamide gel electrophoresis, RNAs extracted from HRVs of either subgroup showed long electropherotype. At least two electropherotypes were distinguished in subgroup I viruses, while five different patterns of viral RNA were found in subgroup II viruses.
Research Institute for Tropical Medicine, Muntinlupa, Metro Manila, Philippines.
I C
IC
Lintag
N
N
Kobayashi
T
T
Urasawa
K
K
Taniguchi
M C
MC
Saniel
S
S
Urasawa
D002675:Child, Preschool; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010679:Philippines / Q000453:epidemiology; D012313:RNA / Q000032:analysis / Q000145:classification; D012401:Rotavirus / Q000145:classification* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000382:microbiology*; D016036:Seroepidemiologic Studies; D012703:Serotyping
D012313:RNA