Silver staining of collagen fibrils in cartilage.
Direct visualization of individual collagen fibrils by light microscopy in human cartilage was achieved by applying a periodic acid-silver methenamine stain on plastic sections. Collagen fibrils, 100 nm in diameter or thicker, were delineated individually by light microscopy and were easily traced for a length beyond 100 microns. Thinner fibrils not readily visible optically were identified if arranged in compact bundles as occurring in the superficial zone of articular cartilage.
Department of Pathology, University of Calgary, Alberta, Canada.
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Hwang
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Saito
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D002356:Cartilage / Q000648:ultrastructure*; D002648:Child; D002675:Child, Preschool; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D006821:Hyaluronoglucosaminidase; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D009993:Osmium Tetroxide; D012834:Silver; D013194:Staining and Labeling
D012834:Silver; D003094:Collagen; D006821:Hyaluronoglucosaminidase; D009993:Osmium Tetroxide