Morphological and quantitative aspects of the development of cytoplasmic granules in concanavalin A-stimulated normal human lymphocytes.
We have studied normal human lymphocytes stimulated by concanavalin A to determine the rate and extent of development of cytoplasmic granules. Morphological studies on stimulated lymphocytes were undertaken at the light and electron microscopic level. The percentage of T lymphocytes bearing different CD antigens (CD3, CD4, and CD8) that also contained cytoplasmic granules was also assessed. Granules were identified in a high percentage of lymphocytes bearing each of the three CD markers studied.
McGirl
A
A
Department of Pathology, University College Dublin, Ireland.
Shea
G
G
Melinn
M
M
McLaughlin
H
H
D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003208:Concanavalin A / Q000494:pharmacology*; D003594:Cytoplasmic Granules / Q000502:physiology / Q000648:ultrastructure*; D006801:Humans; D008213:Lymphocyte Activation / Q000502:physiology*; D008854:Microscopy, Electron / Q000379:methods; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure
D003208:Concanavalin A