Stratum corneum antibodies detected by hemagglutination are not directed against keratin intermediate filaments.
Autoantibodies to stratum corneum (SC) occur in virtually all normal adult human sera. These antibodies may be directed against various antigens of the SC. They have been detected by indirect immunofluorescence, passive hemagglutination (HA), immune adherence, and most recently by enzyme immunoassay and immunoblot methods. The purpose of our study was to examine whether antibodies to SC antigens as detected by passive HA are similar to the keratin intermediate filament (KIF) reactive antibodies. SC antigen preparation was prepared from psoriatic scales by the trypsin-phenol-water (TPW) extraction method. KIFs were prepared by 8 M urea extraction of normal callus or psoriatic scales. The anti-SC antibody titers of normal human sera were determined by passive HA before and after absorption with TPW-SC antigen preparation and upon absorption with KIFs. Similarly, titers of anti-KIF antibodies were determined on absorbed and unabsorbed sera by immunoblot assay. The results of this study indicate that the absorption of the sera with KIFs did not affect the titer of antibodies to TPW-extractable SC antigens whereas the titer of KIF antibodies dropped. KIF-reactive antibodies, on the other hand, were not affected by absorption with TPW-SC antigen, whereas the latter absorbed out the corresponding reactive antibodies. These results indicate that antibodies directed against SC antigen are different from the KIF-reactive antibodies.
Ernest Witebsky Center for Immunology, Department of Microbiology, Buffalo, NY.
S
S
Qutaishat
V
V
Kumar
E H
EH
Beutner
S
S
Jabłońska
D000596:Amino Acids / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis*; D002457:Cell Extracts / Q000276:immunology; D003599:Cytoskeleton / Q000276:immunology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology* / Q000648:ultrastructure; D006384:Hemagglutination / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007382:Intermediate Filaments / Q000032:analysis / Q000276:immunology*; D007633:Keratins / Q000032:analysis / Q000276:immunology*
D000596:Amino Acids; D001323:Autoantibodies; D002457:Cell Extracts; D007633:Keratins