An A alpha Ser-434 to N-glycosylated Asn substitution in a dysfibrinogen, fibrinogen Caracas II, characterized by impaired fibrin gel formation.
We have identified a unique N-glycosylated Asn substitution for a Ser at position 434 of the A alpha chain of an abnormal fibrinogen designated fibrinogen Caracas II. This dysfibrinogen was characterized by impaired fibrin monomer aggregation. Since there were 4 Thr residues immediately following the mutation, a new Asn-X-Thr/Ser-type consensus sequence, Asn-Thr-Thr arose for N-glycosylation of the Asn. The extra oligosaccharide was found to consist mainly of a disialylated biantennary structure comprising 81.9%, while a neutral and a monosialylated biantennary oligosaccharide represented only 3.6% and 14.5%, respectively. The mutation resides in the carboxyl-terminal region of the A alpha chain, which could fold back to form an extra small globular region located near the central region of the molecule (Erickson, H.P., and Fowler, W.E. (1983) Ann. N. Y. Acad. Sci. 408, 146-163; Weisel, H.P., Stauffacher, C.V., Bullitt, E., and Cohen, C. (1985) Science 230, 3124-3133). Therefore, the participation of this region, referred to as an additional central domain or an alpha domain, in fibrin gel formation is strongly implicated.
Division of Hemostasis and Thrombosis Research, Jichi Medical School, Tochigi, Japan.
H
H
Maekawa
K
K
Yamazumi
S
S
Muramatsu
M
M
Kaneko
H
H
Hirata
N
N
Takahashi
N B
NB
de Bosch
Z
Z
Carvajal
A
A
Ojeda
C L
CL
Arocha-Piñango
D000596:Amino Acids / Q000032:analysis; D001216:Asparagine / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D005337:Fibrin / Q000235:genetics* / Q000502:physiology; D005340:Fibrinogen / Q000235:genetics*; D015241:Fibrinogens, Abnormal; D006031:Glycosylation; D006801:Humans; D009154:Mutation; D009844:Oligosaccharides / Q000032:analysis; D010084:Oxidation-Reduction; D012694:Serine / Q000235:genetics*; D013917:Thrombin / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D011503:Transglutaminases / Q000378:metabolism
D000596:Amino Acids; D003432:Cross-Linking Reagents; D015241:Fibrinogens, Abnormal; D009844:Oligosaccharides; C069378:fibrinogen Caracas II; D012694:Serine; D001216:Asparagine; D005337:Fibrin; D005340:Fibrinogen; D011503:Transglutaminases; D013917:Thrombin; D010959:Tissue Plasminogen Activator