Time-dependent cadmium-neurotoxicity in rat brain synaptosomal plasma membranes.
1. The modulation of lipid dynamics and lipid protein interactions were studied in rat brain synaptosomal plasma membranes (SPM) up to 24 hr after exposure to cadmium (Cd). 2. The activity of acetylcholinesterase and adenylate cyclase showed a considerable decrease after 6 hr of Cd exposure, followed by a progressive increase up to 24 hr. 3. SPM chemiluminescence showed a maximum decrease at 12 hr, demonstrating a considerable increase in lipid peroxidation. 4. SPM of Cd-exposed animals showed a statistical significant increase in fluorescence anisotropy parameter [(r0/r) - 1]-1 at 18 and 24 hr compared to SPM of the control, indicating a decrease of membrane fluidity.
Department of Experimental Physiology, School of Medicine, University of Athens, Greece.
C D
CD
Fasitsas
S E
SE
Theocharis
D
D
Zoulas
S
S
Chrissimou
G
G
Deliconstantinos
D000110:Acetylcholinesterase / Q000187:drug effects; D000262:Adenylyl Cyclases / Q000187:drug effects; D000818:Animals; D001921:Brain / Q000187:drug effects*; D002104:Cadmium / Q000633:toxicity*; D002462:Cell Membrane / Q000187:drug effects; D005454:Fluorescence Polarization; D005609:Free Radicals; D008163:Luminescent Measurements; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000187:drug effects*; D013696:Temperature; D013997:Time Factors
D005609:Free Radicals; D008563:Membrane Lipids; D008565:Membrane Proteins; D002104:Cadmium; D000110:Acetylcholinesterase; D000262:Adenylyl Cyclases