Three high-lysine mutations control the level of ATP-binding HSP70-like proteins in the maize endosperm.
The synthesis and deposition of seed storage proteins in maize are affected by several dominant and recessive mutants. The effect of three independent mutations, floury-2 (fl2), Defective endosperm-B30 (De-B30), and Mucronate (Mc), that reduce zein level in the endosperm were investigated. These mutations also control the level of b-70, a polypeptide bound to protein bodies, which is separable into the two isoforms b-70I and b-70II by two-dimensional gel electrophoresis. Both isoforms are overexpressed 10-fold in fl2; however, only b-70I is present in De-B30 and Mc, which contain an amount of total b-70 isoforms fivefold higher than in the wild type. Both b-70I and b-70II resemble heat shock protein (HSP70) in that they bind ATP, cross-react with anti-HSP antibodies, and have N-terminal sequence homology to HSP70. All maize protein body-located b-70 characteristics are typical of those of chaperone-like HSPs. A third protein, b-70III, similar in size to but slightly more acidic than b-70I and b-70II, also binds ATP and reacts with the same antibody, providing evidence for the presence in endosperm extracts of a cytosolic chaperone-like protein. The level of b-70III was not altered by the mutations studied. The results suggested that the repression effect of the three mutations on zein accumulation may be mediated by the alteration of a zein transport or zein assembly process involving b-70I and b-70II.
Istituto di Agronomia, Botanica e Genetica Vegetale, Università Cattolica S. Cuore, Piacenza, Italy.
A
A
Marocco
A
A
Santucci
S
S
Cerioli
M
M
Motto
N
N
Di Fonzo
R
R
Thompson
F
F
Salamini
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D018833:Chaperonins; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D005799:Genes, Dominant; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008239:Lysine / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000276:immunology; D012639:Seeds / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000196:embryology / Q000378:metabolism*; D015028:Zein / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D011506:Proteins; C077221:BIP protein, Arabidopsis; D000255:Adenosine Triphosphate; D015028:Zein; D018833:Chaperonins; D008239:Lysine