Enhanced proliferation of murine T cell lines following interaction of poly(Glu60,Phe40) (GPhe) and antigen-presenting accessory cells. I. GPhe-stimulated enhancement of antigen-dependent proliferative responses.
Following interaction of the random polymer (Glu60,Phe40)n (GPhe) with antigen-presenting accessory cells (APC), unusual costimulatory activities were noted in several murine T cell systems. When GPhe, in contrast with other random copolymers (GT,GL), was added during "inhibition" and T cell "repertoire" studies as a (negative) control to GLA-reactive nonclonal T cell lines of haplotypes H-2d (DCL-2) or H-2bm12, augmentation of T cell proliferation ([3H]thymidine incorporation ([3HT]) to homologous antigen was observed. Augmentation by GPhe was also observed in the response of a GLPhe-reactive (H-2s X H-2d)F1 T cell line and the allogeneic response of the clonal T cell line D10.G4.1. This augmentation was critically dependent on the concentration of adherent accessory cells. Although the mechanism of action of GPhe remains, as yet, undefined, the GPhe-mediated enhancement of DCL-2 (a TH2, H-2d anti-GLA, T cell line) proliferation was not dependent upon the production of either IL-1 or IL-6 by accessory cells. In addition, enhanced DCL-2 proliferation was not accompanied by a significant increase in detectable IL-4 release.
Department of Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
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Liu
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Grun
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Maurer
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001685:Biological Factors; D002460:Cell Line; D003429:Cross Reactions; D016207:Cytokines; D007375:Interleukin-1 / Q000502:physiology; D015847:Interleukin-4 / Q000096:biosynthesis / Q000502:physiology; D015850:Interleukin-6 / Q000502:physiology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010455:Peptides / Q000276:immunology*; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000276:immunology; D011099:Polyglutamic Acid / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D001685:Biological Factors; D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D010455:Peptides; D015847:Interleukin-4; D011099:Polyglutamic Acid; C023843:copolymer Glu(60)Phe(40); D010649:Phenylalanine