Signal transduction through class I MHC by a monoclonal antibody that detects multiple murine and human class I molecules.
We have generated a hamster anti-mouse class I reactive mAb that is capable of activating T cells in the presence of the cofactor PMA, as assayed by both IFN-gamma production and cellular proliferation. This mAb detects an epitope present on the majority of murine class I molecules, with the known exceptions of H-2Kk and H-2Kq, and is therefore not beta 2-microglobulin-specific. It also recognizes multiple human class I molecules. The epitope recognized by this antibody maps to the class I alpha 1 domain. The activation properties of this mAb are not mediated exclusively through the glycosylphosphatidylinositol-linked Qa-2 molecule, as the antibody activates spleen cells from Qa-2 negative strains. Although class I molecules are not usually considered as activation Ag, these data demonstrate their potential for involvement in signal transduction.
Committee on Immunology, University of Chicago, Ben May Institute, IL 60637.
B A
BA
Houlden
S M
SM
Widacki
J A
JA
Bluestone
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000939:Epitopes / Q000276:immunology; D006680:HLA Antigens / Q000032:analysis / Q000502:physiology*; D006413:Hematopoietic System / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000502:physiology*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D015398:Signal Transduction / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I