The occurrence of neuropeptides at different stages of DBM-induced heterotopic bone formation.
In developing heterotopic bone in the rat, induced by allogeneic bone matrix, we immunohistochemically detected nerves containing substance P (SP), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP) and tyrosine hydroxylase (TH). After 10 days they were dicernible amidst differentiating chondroblastoid cells in fibrous tissue around and within the implants. Over the next 3 weeks, the nerves increased in number and gradually attained a shape and distribution resembling normal osseal nerves; varicose fibres frequently occurred in periosteum-like fibrous tissue and bone marrow adjacent to newly formed bone. At 8 weeks, NPY-fibres increased, particularly in the marrow and this abundance of NPY fibres remained at 16 weeks. VIP-immunoreactive fibres were only observed in the surrounding periosteum-like fibrous tissue 4-6 weeks after implantation. These observations, in combination with recent findings of receptors to neuropeptides on bone cells, suggest a neurogenic influence on physiological processes in bone tissue.
Department of Orthopaedic Surgery, Karolinska Hospital, Stockholm, Sweden.
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Bjurholm
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Kreicbergs
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Dahlberg
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M
Schultzberg
D000818:Animals; D001846:Bone Development / Q000502:physiology*; D001857:Bone Matrix / Q000637:transplantation*; D001842:Bone and Bones / Q000294:innervation* / Q000502:physiology; D002113:Calcification, Physiologic; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000378:metabolism; D016042:Transplantation, Heterotopic; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D009478:Neuropeptide Y; D009479:Neuropeptides; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D014446:Tyrosine 3-Monooxygenase; D015740:Calcitonin Gene-Related Peptide