The ontogeny of specific retrograde transport of nerve growth factor by motoneurons of the brainstem and spinal cord.
Radiolabeled Nerve Growth Factor (NGF) was injected into either the mandibular process of the first visceral arch or the limb bud of chick embryos at Days 3.5-14 or Days 4-13 of incubation, respectively. Control embryos received injections of labeled cytochrome-C or labeled NGF plus an excess of unlabeled NGF. The tissues were then processed for autoradiography. The 125I-NGF was retrogradely transported by motoneurons of the trigeminal (V) motor nucleus on Days 3.5-8 of incubation, but not at later stages. Similar transport was seen in motoneurons of the spinal cord lateral motor column from Days 4-10 of incubation, but not at later stages. Sensory neurons of the V ganglion and of the dorsal root ganglia transported NGF at all injection ages. In no instance was the 125I-cytochrome-C transported by sensory or motor neurons. The injection of an excess of cold NGF along with labeled NGF resulted in no evidence of retrograde transport of the labeled NGF indicating that the transport was saturable. The time of transport by these brainstem and spinal cord motoneurons corresponds closely to the points during development at which they have been found to exhibit specific NGF binding. The present results, then, provide further evidence for a possible biological role for NGF during early developmental stages of these motoneuron populations.
Wayne
D B
DB
Department of Neuroscience, University of Florida College of Medicine, Gainesville 32610.
Heaton
M B
MB
D000344:Afferent Pathways / Q000033:anatomy & histology / Q000166:cytology / Q000196:embryology; D000818:Animals; D001370:Axonal Transport; D001933:Brain Stem / Q000033:anatomy & histology / Q000166:cytology / Q000196:embryology*; D002642:Chick Embryo; D005152:Facial Muscles / Q000196:embryology; D009046:Motor Neurons / Q000166:cytology / Q000502:physiology*; D009414:Nerve Growth Factors / Q000378:metabolism*; D013116:Spinal Cord / Q000033:anatomy & histology / Q000166:cytology / Q000196:embryology*; D014276:Trigeminal Nerve / Q000033:anatomy & histology / Q000166:cytology / Q000196:embryology
D009414:Nerve Growth Factors