Heparin-binding neurotrophic factor (HBNF) and MK, members of a new family of homologous, developmentally regulated proteins.
A partial rat cDNA clone coding for a novel neurotrophic factor HBNF was isolated. Nucleotide sequence determination, in combination with the known N-terminal sequence of rat HBNF, allowed deduction of the amino acid sequence of the first 102 residues of mature rat HBNF. HBNF shares high structural homology (55%) with the MK protein (Tomomura et al., J. Biol. Chem. 265, 10765, 1990). Complete alignment of 9 cysteine residues suggests further that the two proteins have similar 3-dimensional structures. HBNF was reported to stimulate neurite outgrowth in neurons and to be expressed in a developmentally regulated manner in the rat brain. MK mRNA was found in retinoid acid-induced teratocarcinoma cells and during early development of the mouse embryo, but no biological activity for MK is yet known. These data suggest that HBNF and MK are members of a novel family of structurally and probably functionally related proteins.
Kovesdi
I
I
Medical Research Division, American Cyanamid Company, Pearl River, NY 10965.
Fairhurst
J L
JL
Kretschmer
P J
PJ
Böhlen
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics*; D016207:Cytokines / Q000235:genetics*; D051379:Mice; D000078792:Midkine; D008969:Molecular Sequence Data; D005810:Multigene Family; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000235:genetics*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D012689:Sequence Homology, Nucleic Acid
D002352:Carrier Proteins; D016207:Cytokines; C066254:MDK protein, human; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D011506:Proteins; C066845:pleiotrophin; D000078792:Midkine; D012313:RNA