DNA restriction polymorphism in wild isolates of Spodoptera frugiperda nuclear polyhedrosis virus.
Restriction endonuclease analysis was used to examine variation in DNA of 22 wild isolates of Spodoptera frugiperda nuclear polyhedrosis virus (SfNPV). Eleven of the 15 isolated from Louisiana were distinguishable based on restriction fragment profiles from the enzymes BamHI, HindIII, and EcoRI. There was significant genetic variation in SfNPV isolates within single agricultural fields. Nucleotide sequence divergence values, based on restriction fragment profiles, indicated that genetic variation among isolates foreign to Louisiana (Ohio, Ecuador, Mexico, Georgia, Colombia, and Venezuela) was greater than that among the Louisiana isolates. However, certain foreign isolates were similar to or identical with Louisiana isolates. Genetic variation of the viral DNA was not influenced by the insect's host plan species.
Shapiro
D I
DI
Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge 70803.
Fuxa
J R
JR
Braymer
H D
HD
Pashley
D P
DP
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis*; D014644:Genetic Variation; D009036:Moths; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D004279:DNA, Viral