Angiogenesis is associated with collagenous protein synthesis and degradation in the chick chorioallantoic membrane.
Extracts of the chick embryo chorioallantoic membrane (CAM) obtained from 7-20 day old embryos, contained enzyme activity that could degrade type IV collagen. Peak enzyme activity was observed on days 8-10 of embryogenesis, which coincides with the stage of maximum angiogenesis. This activity decreased to lowest values at days 13-15 and increased thereafter up to day 20. Maximum rate of collagen biosynthesis in CAM was observed between days 7 and 10, with a drastic decrease at day 12, when vascular density has reached a plateau. The type IV collagen-degrading activity of CAM was of the metalloprotease type, since it was inhibited by 1,10-phenanthroline and EDTA but was also partially inhibited by serine and thiol protease inhibitors.
Department of Pharmacology, University of Patras Medical School, Greece.
E
E
Missirlis
G
G
Karakiulakis
M E
ME
Maragoudakis
D000482:Allantois / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation; D002642:Chick Embryo; D002823:Chorion / Q000378:metabolism; D003094:Collagen / Q000096:biosynthesis / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9; D003012:Microbial Collagenase / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D013379:Substrate Specificity
D003094:Collagen; D003012:Microbial Collagenase; D020780:Matrix Metalloproteinase 9