Effects of transforming growth factor beta 1 and basic fibroblast growth factor on lipoprotein lipase activity in primary cultures of chicken (Gallus domesticus) adipocyte precursors.
1. The effect of TGF-beta and bFGF on lipoprotein lipase activity in chicken adipocyte precursors was investigated. 2. Lipoprotein lipase activity was reduced by up to 80% by incubation with TGF-beta whereas bFGF had no effect. 3. Contrary to that found with the 3T3-L1 preadipocyte cell line it was not necessary for TGF-beta to be present prior to the start of differentiation in order to be effective. 4. Incubation of adipocyte precursors with actinomycin D abolished the effect of TGF-beta suggesting that synthesis of a protein effector is required. 5. These results indicate differences in responsiveness to TGF-beta and bFGF between primary chicken adipocyte precursors and some preadipocyte cell lines.
AFRC Institute for Animal Physiology and Genetics Research, Department of Cellular and Molecular Biology, Roslin, Midlothian, U.K.
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SC
Butterwith
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Gilroy
D000273:Adipose Tissue / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002645:Chickens; D003609:Dactinomycin / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000502:physiology*
D016212:Transforming Growth Factor beta; D003609:Dactinomycin; D005346:Fibroblast Growth Factors; D008071:Lipoprotein Lipase