The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis.
PCNA is a nuclear protein that is synthesized in late G1 and S phases of the cell cycle and is, therefore, correlated with the cell proliferative state. A new monoclonal antibody (PC10) to genetically engineered PCNA has been shown to label proliferating cells in formalin-fixed paraffin-embedded normal human tissues. Previous studies in lymphomas, using various markers of cell proliferation, have shown a strong correlation between indices of cell proliferation and histological grade. These studies have shown that within each histological subtype there is often a wide range of proliferative indices and that these may be of some prognostic significance. Thirty-one gastrointestinal lymphomas were studied. Our results show that there is a good correlation between PC10 index and histological grade of tumour (0.01 P greater than P greater than 0.001) and also a significant relationship between PC10 index and S+G2+M phase fraction as measured by flow cytometric analysis (r2 = 0.62; P less than 0.01). Twenty-three cases were available for survival analysis. In these cases a high PC10 score correlated with poor survival (P = 0.04). Based on this series, it appears that there is a significant relationship between PC10 index and histological grade, and between PC10 index and S+G2+M phase as measured by flow cytometric analysis. In addition, our results suggest that a high PC10 index is an adverse prognostic factor in primary gastrointestinal lymphoma.
Woods
A L
AL
Department of Histopathology, UMDS, Guy's Hospital, London, UK.
Hall
P A
PA
Shepherd
N A
NA
Hanby
A M
AM
Waseem
N H
NH
Lane
D P
DP
Levison
D A
DA
D002453:Cell Cycle; D002455:Cell Division; D005434:Flow Cytometry; D016195:G2 Phase; D005770:Gastrointestinal Neoplasms / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008223:Lymphoma / Q000276:immunology* / Q000473:pathology; D008677:Metaphase; D009687:Nuclear Proteins / Q000276:immunology*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen; D016196:S Phase
D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen