[Pentobarbital interaction with the GABA-activated ion channels of the neurons in the rat cerebellum].
GABA and barbiturate-activated currents of isolated single neurons in the rat cerebellum were studied by means of the concentration clamp, voltage clamp and intracellular perfusion methods. The dissociation constant (Kd) was 3 +/- 0.8.10(-5) M. Pentobarbital potentiated the GABA-induced conduction of isolated neurons. The dose-effect for GABA shifted along the abscissa axes. Optimal concentrations which potentiated the GABA effect were within 10(-6)-10(-4) M. The pentobarbital concentrations above 5.10(-4) M without GABA activated the Cl conductance. The short-time conductance during fast pentobarbital washing off was found to increase.
A E
AE
Valeev
O A
OA
Kryshtal'
N I
NI
Chernevskaia
D000818:Animals; D002531:Cerebellum / Q000187:drug effects* / Q000502:physiology; D002712:Chlorides / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D010424:Pentobarbital / Q000494:pharmacology*; D051381:Rats; D013268:Stimulation, Chemical; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D002712:Chlorides; D007473:Ion Channels; D005680:gamma-Aminobutyric Acid; D010424:Pentobarbital
Vzaimodeĭstvie pentobarbitala s GAMK-aktiviruemymi ionnymi kanalami neĭronov mozzhechka krys.