Damage and repair induced by bleomycin in the domain of human amplified MYC oncogenes.
Growing monolayer COLO320 human cells having a 30- to 40-fold amplification of a MYC domain were pulse treated for 15 min with increasing doses of bleomycin (BLM). Cellular DNA was extracted at the end of the BLM treatment or after an interval of 30 min, during which the cells were allowed to repair the DNA damage at 37 degrees C in culture medium without BLM. Damage and repair in total cellular DNA was assessed by alkaline unwinding and by neutral and alkaline gel electrophoresis. The response to BLM in the domain of MYC oncogene was evaluated by Southern blotting of EcoRI-digested DNAs separated by neutral or alkaline gel electrophoresis. We found that MYC domains from COLO320HSR showed a higher frequency of double-strand DNA breaks than MYC domains from COLO320DM cells. At the level of total cellular DNA, both cell lines showed the same frequency of double-strand nicks. No repair of double-strand breaks was observed. Total DNA from COLO320HSR cells was more sensitive to single-strand breakage than DNA from COLO320DM. At the gene level, the frequency of single-strand scissions was higher in COLO320DM than in COLO320HSR MYC domains. Both cell lines had good capability to close single-strand scissions. However, at high BLM doses the damage was more efficiently repaired by COLO320DM cells. We propose that chromatin organization in total cellular DNA and in the amplified MYC domain probably plays an important role in the DNA response to BLM.
Bianchi
N O
NO
IMBICE, La Plata, Argentina.
Bianchi
M S
MS
López-Larraza
D
D
Alitalo
K
K
de la Chapelle
A
A
D000230:Adenocarcinoma; D001761:Bleomycin / Q000494:pharmacology*; D002460:Cell Line; D003110:Colonic Neoplasms; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005784:Gene Amplification; D006801:Humans; D007700:Kinetics; D009857:Oncogenes / Q000187:drug effects*; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D001761:Bleomycin; D011505:Protein-Tyrosine Kinases