Intercellular adhesion molecule-1 (ICAM-1, CD 54) is associated with actin-filaments.
Intercellular adhesion molecule 1 (ICAM-1, CD 54) is a membrane associated glycoprotein involved in cell-cell interactions of the immune system. Detergent extraction of cultured human fibroblasts--stimulated with Interferon-gamma (IFN-gamma) for ICAM-1-expression--under conditions that stabilize actin in the filamentous (actin-F) form (NaF-buffer, phalloidine) resulted in greater retention of ICAM-1 in the detergent insoluble phase, containing the cytoskeletal matrix, compared to actin-F-destabilizing conditions (KCl-buffer). We further examined the in vitro ICAM-1 association with actin using immunoaffinity purified ICAM-1, prepared from either normal human tonsils or a spleen derived from a patient with Non-Hodgkin's lymphoma. ICAM-1 from both sources demonstrated binding to actin coated polystyrene surfaces. Our findings suggest that ICAM-1 interacts with actin-F and may use similar mechanisms described with other adhesion molecules for membrane-cytosol communication.
Institute for Hygiene, University of Innsbruck, Austria.
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Dierich
D000199:Actins / Q000378:metabolism; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000302:isolation & purification / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D015932:Complement C3c / Q000378:metabolism; D003599:Cytoskeleton / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1; D011189:Potassium Chloride / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology; D001616:beta-Galactosidase / Q000378:metabolism
D000199:Actins; D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1; D011189:Potassium Chloride; D015932:Complement C3c; D012969:Sodium Fluoride; D001616:beta-Galactosidase