Transport systems for polyamines in the established renal cell line LLC-PK. Polarized expression of an Na(+)-dependent transporter.
We present evidence for the existence of an Na(+)-dependent transporter and an Na(+)-independent transporter for polyamines in LLC-PK1 cells. Both transporters could be discriminated by their sensitivity to inhibitors, particularly rho-chloromercuriphenyl sulphate and various polycationic molecules. By using cell monolayers grown on a permeable filter support, we have found that the Na(+)-dependent polyamine uptake occurred preferentially from the basolateral side. The Na(+)-independent uptake, on the other hand, occurred to the same extent from either the apical or the basolateral side.
Physiological Laboratory, Katholicke Universiteit Leuven, Belgium.
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L
Van Den Bosch
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De Smedt
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L
Missiaen
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JB
Parys
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R
Borghgraef
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D000518:Eflornithine / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D007668:Kidney; D007700:Kinetics; D011073:Polyamines / Q000378:metabolism*; D011107:Polylysine / Q000494:pharmacology; D011700:Putrescine / Q000378:metabolism*; D012430:Ruthenium Red / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology
D005839:Gentamicins; D011073:Polyamines; D012430:Ruthenium Red; D011107:Polylysine; D002731:4-Chloromercuribenzenesulfonate; D012964:Sodium; D011700:Putrescine; D000518:Eflornithine