Oral transmission of Jamestown Canyon virus by Aedes provocans mosquitoes from northeastern New York.
Aedes provocans were allowed to feed on a bloodmeal containing 5.6 log10 TCID50/ml of Jamestown Canyon (JC) virus. After 14 days of incubation at 21 degrees C and 80% RH, 100% (36/36) were midgut infected, 50% (18/36) developed disseminated infections and 50% (9/18) of the latter specimens transmitted virus to capillary tubes. When mosquitoes were intrathoracically inoculated with 6.1 log10 TCID50/ml of JC virus, 100% (40/40) became disseminated infected and 95% (38/40) transmitted virus after 12 days of incubation. A midgut escape barrier was recognized as the major barrier to JC virus transmission in orally infected Ae. provocans.
Boromisa
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Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Grayson
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MA
D000818:Animals; D009033:Culicidae / Q000382:microbiology*; D002141:Encephalitis Virus, California / Q000302:isolation & purification; D004670:Encephalitis, California / Q000635:transmission*; D005260:Female; D007303:Insect Vectors; D009518:New York; D012463:Saliva / Q000382:microbiology