Release of 2-thiobarbituric acid reactive products from glutamate, deoxyuridine or DNA during autoxidation of dopamine in the presence of copper ions.
Cytotoxic effects of catecholamine are thought to be caused by the formation of 0-semiquinone and superoxide radicals. Dopamine in the presence of copper ions releases aldehydic products from glutamate, deoxyuridine or DNA capable of reacting with 2-thiobarbituric acid (TBA). The formation of TBA reactive products (TBAR) was concentration dependent of both dopamine and copper ion. Complete inhibition of formation of TBAR from glutamate, deoxyuridine or DNA was observed in the presence of thiourea and catalase. Mannitol, albumin and superoxide dismutase offered substantial protection. The present data indicate that dopamine in the presence of copper ions can lead to the formation of reactive hydroxyl radicals which can release aldehydic products from glutamate, deoxyuridine or DNA capable of reacting with TBA.
Industrial Toxicology Research Centre, Lucknow, India.
G S
GS
Rao
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000494:pharmacology; D003300:Copper / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D003857:Deoxyuridine / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D010084:Oxidation-Reduction; D013858:Thiobarbiturates / Q000378:metabolism*; D013890:Thiourea / Q000494:pharmacology
D000447:Aldehydes; D005971:Glutamates; D013858:Thiobarbiturates; D018698:Glutamic Acid; D003300:Copper; D004247:DNA; D002374:Catalase; D013890:Thiourea; C029684:thiobarbituric acid; D004298:Dopamine; D003857:Deoxyuridine