Structural, functional, and antigenic differences between bovine heart endothelial CD36 and human platelet CD36.
Endothelial cell CD36 (glycoprotein IV) has been purified from bovine heart tissue by detergent partitioning and immunoaffinity chromatography. Bovine CD36 differs from human CD36 in its apparent mass (85 versus 88 kDa), primary structure, and immunological cross-reactivity. Of the 18 N-terminal residues identified, 17 conformed to the human CD36 sequence. Mouse monoclonal antibodies E-1 and 8A6 defined bovine- and human-specific epitopes, respectively. Because human CD36 has been identified as a receptor for erythrocytes infected with the malaria parasite Plasmodium falciparum, we examined the ability of bovine CD36 to bind infected erythrocytes. Bovine CD36, unlike human CD36, did not bind infected erythrocytes, suggesting that human CD36-specific structural features are responsible for recognition of the infected erythrocyte ligand.
Greenwalt
D E
DE
Department of Chemistry, San Jose State University, California 95192-0101.
Watt
K W
KW
Hasler
T
T
Howard
R J
RJ
Patel
S
S
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000302:isolation & purification*; D000943:Antigens, Differentiation / Q000235:genetics / Q000302:isolation & purification*; D001792:Blood Platelets / Q000276:immunology*; D018955:CD36 Antigens; D002417:Cattle; D002462:Cell Membrane / Q000276:immunology; D004730:Endothelium, Vascular / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D008861:Microsomes / Q000276:immunology*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology; D010980:Platelet Membrane Glycoproteins / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid
D015703:Antigens, CD; D000943:Antigens, Differentiation; D018955:CD36 Antigens; D010980:Platelet Membrane Glycoproteins