Effects of beta 2-adrenergic stimulants on the progesterone and oestradiol-17 beta serum levels in pseudopregnant rats.
In CFY rats pseudopregnancy (PSP) was induced by mechanical stimulation of cervix uteri. On the days 1 or 7 of PSP indvelling catheter was built in one of the jugular veins and blood was collected daily until 7th or 14th days of PSP respectively, meanwhile the animals were daily injected with clenbuterol (0.1 mg/kg bw), fenoterol (0.5 mg/kg bw), ritodrin (1.0 mg/kg bw), propranolol (2.0 mg/kg bw) or 1.0 ml/kg bw 0.9% NaCl subcutaneously. From the blood samples progesterone (P) and oestradiol-17 beta (E2) were determined by RIA. beta 2-adrenergic agonists did not influence the serum levels of P either in the first or in the second half of PSP and the propranolol failed to alter also the serum levels of P during the PSP. Clenbuterol and ritodrin, however, decreased the serum levels of E2 in the first half of PSP, while in the second half of PSP fenoterol and ritodrin elevated, the propranolol diminished it. It was supposed that in PSP rats beta 2-adrenergic mechanism has a more pronounced effects on the theca-interstitial cells than that of the luteal cells.
Second Department of Obstetrics and Gynecology, Semmelweis University Medical School, Budapest, Hungary.
B
B
Zsolnai
B
B
Varga
E
E
Horváth
E
E
Stark
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects; D004958:Estradiol / Q000097:blood*; D005260:Female; D011374:Progesterone / Q000097:blood*; D011555:Pseudopregnancy; D011863:Radioimmunoassay; D051381:Rats
D000318:Adrenergic beta-Agonists; D011374:Progesterone; D004958:Estradiol