In vitro cytophotometry evaluation of gamma-radiation effects on human sperm cell by acridine orange staining.
Effects of gamma-radiations on human sperm nuclei in vitro were studied by cytophotometrical quantification on fixed smears stained by acridine orange some of which had undergone heat-treatment. Following irradiations at different doses (0.0, 2.5, 5.0, 7.5 gy) using a cobalt source, changes in distribution of acridine orange fluorescences emitted by nuclei (total fluorescence greater than 515 nm versus green fluorescence = 525 nm) appeared to be dose-dependent. Heat-treatment of irradiated heads was confirmed to be a complementary procedure necessary for a good evaluation of the initial degree in chromatin compactness. Chromatin alterations related to changes in emitted fluorescence could be due to the initial fragility of the DNA-nucleoprotein complex. These preliminary results suggest that the normality of sperm chromatin could be used as a biologic dosimeter.
Faculté de Médecine Broussais Hôtel-Dieu, UER Biomédicale, Paris, France.
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Neveux
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Dadoune
D000165:Acridine Orange; D002843:Chromatin / Q000528:radiation effects; D003035:Cobalt; D003592:Cytophotometry; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D013077:Sperm Head / Q000528:radiation effects; D013094:Spermatozoa / Q000528:radiation effects* / Q000648:ultrastructure; D013194:Staining and Labeling
D002843:Chromatin; D003035:Cobalt; D000165:Acridine Orange