DNA investigations on fetal material from paternity cases.
Three paternity cases have been investigated where DNA was extracted from fetuses (age: 8-10 weeks old) after interruption of pregnancy. In each case it was possible to clearly identify the putative father using 5 or 6 single locus probes (SLP's). Fetal bands (SLP) could be clearly identified from mixtures of placental and fetal DNA by comparison with the maternal and paternal bands. However, it was very difficult to resolve the fragment patterns of tissue mixtures with one multi locus probe (MLP), because of band overlap. Another advantage of using SLP's was that biostatistical calculations could be carried out and very informative Essen-Möller values for the probability of paternity were obtained.
Institut für Rechtsmedizin, Westfälische Wilhelms-Universität Münster, Federal Republic of Germany.
P
P
Wiegand
J
J
Lorente
B
B
Brinkmann
D000032:Abortion, Therapeutic; D000293:Adolescent; D001345:Autoradiography; D002650:Child Abuse, Sexual / Q000175:diagnosis; D002871:Chromosome Banding / Q000379:methods* / Q000592:standards; D016172:DNA Fingerprinting / Q000379:methods* / Q000592:standards; D005069:Evaluation Studies as Topic; D005260:Female; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D007179:Incest; D010334:Paternity; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D011261:Pregnancy Trimester, First