Identification and characterization of monoclonal antibodies reactive with bovine, caprine and ovine T-lymphocyte determinants by flow microfluorimetry.
Comparative flow microfluorimetric (FMF) analysis was used to identify and characterize 27 monoclonal antibodies (MoAbs) reactive with bovine T-lymphocytes. Determinants present on all circulating T-lymphocytes were recognized by 11 MoAbs, 8 of which blocked E rosette formation. Determinants present on only the BoCD4+ T-lymphocyte subset were detected by 9 MoAbs, while determinants restricted to the BoCD8+ T-lymphocyte subset were recognized by 7 MoAbs. Competitive labeling experiments demonstrated that determinants recognized by subset-specific MoAbs were present on BoCD4 or BoCD8 molecules. Comparative studies revealed that some determinants, both pan-T specific and subset-specific, were conserved on homologous (orthologous) molecules expressed on leukocytes from other species of ruminants. Polymorphism was evident with several determinants.
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington St. University, Pullman 99164-7040.
R A
RA
Larsen
M L
ML
Monaghan
Y H
YH
Park
M J
MJ
Hamilton
J A
JA
Ellis
W C
WC
Davis
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D005434:Flow Cytometry; D005470:Fluorometry; D006041:Goats; D007132:Immunoglobulin Isotypes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011110:Polymorphism, Genetic; D012397:Rosette Formation; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000939:Epitopes; D007132:Immunoglobulin Isotypes