Neutralization of bovine herpesvirus type 4 by pairs of monoclonal antibodies raised against two glycoproteins and identification of antigenic determinants involved in neutralization.
In infected cattle, bovine herpesvirus type 4 (BHV-4) induces an immune response with low neutralizing antibody levels or in the absence of such antibodies. For the study of this phenomenon, monoclonal antibodies (MAbs) raised against two BHV-4 glycoproteins identified previously (150K/120K/51K and 120K/16.5K) were used in neutralization tests. None of the MAbs except for MAb 16 could neutralize alone; pairs of MAbs against the 150K/120K/51K and 120K/16.5K glycoproteins were able to neutralize BHV-4 infectivity. MAbs involved in neutralization were used in competitive binding assays to identify epitopes relevant for BHV-4 neutralization. These MAbs showed a low avidity and a weak neutralizing activity, and they partially decreased BHV-4 attachment to cells. These results suggest that the BHV-4 glycoprotein domains involved in viral infectivity are poorly exposed to the immune system.
Department of Virology-Immunology, Faculty of Veterinary Medicine, University of Liège, Belgium.
J
J
Dubuisson
J
J
Guillaume
D
D
Boulanger
E
E
Thiry
M
M
Bublot
P P
PP
Pastoret
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001667:Binding, Competitive; D002417:Cattle; D002460:Cell Line; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D006564:Herpesviridae / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D009500:Neutralization Tests; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D006023:Glycoproteins; D007074:Immunoglobulin G; D014764:Viral Proteins