[Effective synthesis of oligo(poly)deoxyribonucleotides using an H-phosphonate method in plastic microcolumn].
A facile technique of manual oligonucleotide synthesis via H-phosphonate approach is developed. Syntheses carried out in pipette tips with siliconised glasswool filters take 3-3.5 min per cycle with 97-98% yields per condensation. The method was used to synthesize 12-55-mers: T7 and PL promoter regions, gene of the signal peptide of the E. coli OmpA protein, oligonucleotides coding for amino acid sequences 94-105 of preS1- and 133-143 of preS2-regions of hepatitis B virus, hybridisation probes, sequencing primers, oligonucleotides for site-directed mutagenesis, etc.
M G
MG
Isaguliants
V V
VV
Samoshin
I V
IV
Makeeva
V D
VD
Smirnova
D001483:Base Sequence; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics; D015488:HIV Antigens / Q000235:genetics; D015497:HIV-1 / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology; D008722:Methods; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000235:genetics; D009943:Organophosphorus Compounds; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis* / Q000235:genetics
D000939:Epitopes; D015488:HIV Antigens; D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; D011089:Polydeoxyribonucleotides
Effektivnyĭ sintez oligo(poli)dezoksiribonukleotidov H-fosfonatnym metodom v plastikovoĭ mikrokolonke.