Interaction with functional membrane proteins--a common mechanism of toxicity for lipophilic environmental chemicals?
1. The effects of several phenols, anilines and aliphatic alcohols on yeast plasma membrane H(+)-ATPase and purine transport system as well as on Na+, K(+)-ATPase and adenosine uptake by Chinese hamster ovary cells (CHO) were investigated. 2. In all cases an inhibition was observed, which could be correlated with the octanol/water partition coefficients of the substances tested, thus making quantitative structure-activity predictions possible. 3. The observed effects correlated well with the influence of the chemicals on cell growth. 4. The results suggest a common mechanism of toxicity by the action of hydrophobic xenobiotics on biomembranes.
Ahlers
J
J
Federal Environmental Agency, Berlin, F.R.G.
Cascorbi
I
I
Forêt
M
M
Gies
A
A
Köhler
M
M
Pauli
W
W
Rösick
E
E
D000251:Adenosine Triphosphatases / Q000187:drug effects; D000438:Alcohols / Q000633:toxicity; D000814:Aniline Compounds / Q000633:toxicity; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D004785:Environmental Pollutants / Q000633:toxicity*; D008051:Lipid Bilayers / Q000378:metabolism*; D008565:Membrane Proteins / Q000187:drug effects*; D010636:Phenols / Q000633:toxicity; D012441:Saccharomyces cerevisiae / Q000187:drug effects
D000438:Alcohols; D000814:Aniline Compounds; D004785:Environmental Pollutants; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010636:Phenols; D000251:Adenosine Triphosphatases