Transcription from the rha operon psr promoter.
S1 nuclease mapping experiments performed with RNA extracted from cell lines that were unable to metabolize L-rhamnose demonstrated that L-rhamnose and not a metabolite was the inducer of the L-rhamnose operons of Escherichia coli. In vitro transcription studies showed that purified RhaR activates transcription from the psr promoter in the presence of L-rhamnose. In the absence of L-rhamnose, RhaR binds to the psr promoter but does not activate transcription until L-rhamnose is added.
Department of Biochemistry, Brandeis University, Waltham, MA 02254.
J F
JF
Tobin
R F
RF
Schleif
D002460:Cell Line; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009876:Operon; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D012210:Rhamnose / Q000378:metabolism*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D015719:Single-Strand Specific DNA and RNA Endonucleases; D012210:Rhamnose