Production and characterisation of monoclonal antibodies directed against different epitopes of human thyroid stimulating hormone.
We have produced monoclonal antibodies (mAbs) to human thyroid stimulating hormone (hTSH) and selected five that specifically recognize hTSH and do not cross-react with the other human glycoprotein hormones such as luteinizing hormone (LH), chorionic gonadotropin (CG), follicle stimulating hormone (FSH). All of the antibodies were of the IgG1 subclass with affinities ranging from 5.3 X 10(8) to 1.9 X 10(10) mol-1.l; they could be assigned to two subgroups on the basis of their epitope specificity.
INSERM U. 283, Hôpital Cochin, Paris, France.
J M
JM
Braun
J
J
Charreire
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D001667:Binding, Competitive; D000939:Epitopes; D006801:Humans; D011989:Receptors, Thyrotropin / Q000276:immunology; D013972:Thyrotropin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011989:Receptors, Thyrotropin; D013972:Thyrotropin