Conservation in structure of TOC1 transposons from Chlamydomonas reinhardtii.
TOC1 transposons from Chlamydomonas reinhardtii have an unusual arrangement of long terminal repeats. Polymorphic regions between TOC1 transposons were identified by restriction mapping on Southern blots. The variation in size of an internal MluI fragment defines two subclasses of TOC1 elements. Full-length cloned members of each subclass of TOC1 element were compared by electron microscope heteroduplex analysis. The cloned elements were co-linear over their entire length with no large sequence discontinuities. Base substitutions and small insertion/deletion events of less than 50 bp are responsible for forming the two subclasses of TOC1 elements.
Department of Molecular and Plant Biology, University of Geneva, Switzerland.
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Day
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Rochaix
D000818:Animals; D005075:Biological Evolution; D015139:Blotting, Southern; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA; D004251:DNA Transposable Elements; D005810:Multigene Family; D009692:Nucleic Acid Heteroduplexes; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D009692:Nucleic Acid Heteroduplexes; D004247:DNA