Determination of a molecular map position for Hyp using a new interspecific backcross produced by in vitro fertilization.
We have established a Mus spretus/Mus musculus domesticus interspecific backcross segregating for two X-linked mutant genes, Ta and Hyp, using in vitro fertilization. The haplotype of the recombinant X chromosome of each of 241 backcross progeny has been established using the X-linked anchor loci Otc, Hprt, Dmd, Pgk-1, and Amg and the additional probes DXSmh43 and Cbx-rs1. The Hyp locus (putative homologue of the human disease gene hypophosphatemic rickets, HYP) has been incorporated into the molecular genetic map of the X chromosome. We show that the most likely gene order in the distal portion of the mouse X chromosome is Pgk-1-DXSmh43-Hyp-Cbx-rs1-Amg, from proximal to distal. The distance in centimorgans (mean +/- SE) between DXSmh43 and Hyp was 2.52 +/- 1.4 and that between Hyp and Cbx-rs1 was 1.98 +/- 1.39. Thus closely linked flanking markers for the Hyp locus that will facilitate the molecular characterization of the gene itself have been defined.
Kay
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G
Section of Comparative Biology, MRC Clinical Research Centre, Harrow, Middlesex, United Kingdom.
Thakker
R V
RV
Rastan
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S
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D015139:Blotting, Southern; D002118:Calcium / Q000097:blood; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D015342:DNA Probes / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro; D008040:Genetic Linkage / Q000235:genetics*; D006801:Humans; D007015:Hypophosphatemia, Familial / Q000235:genetics*; D008297:Male; D051379:Mice; D009154:Mutation / Q000235:genetics; D010710:Phosphates / Q000097:blood; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic / Q000235:genetics; D014960:X Chromosome
D015342:DNA Probes; D010710:Phosphates; D000469:Alkaline Phosphatase; D002118:Calcium