Intracellular localization of pp60c-src in human platelets.
We have studied the intracellular distribution of the proto-oncogene product pp60c-src in human platelets by immunofluorescence microscopy and by immunogold electron microscopy on frozen thin sections. Virtually all of the immunoreactive pp60c-src was found to be associated with the plasma membrane and with membranes of the surface-connected canalicular system. This localization suggests that pp60c-src may relay signals from an as yet unidentified cell surface protein to intracellular targets.
Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720.
J E
JE
Ferrell
J A
JA
Noble
G S
GS
Martin
Y V
YV
Jacques
D F
DF
Bainton
D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000378:metabolism; D015539:Platelet Activation; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016392:Proto-Oncogene Proteins pp60(c-src); D013917:Thrombin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D016392:Proto-Oncogene Proteins pp60(c-src); D013917:Thrombin