The primary B cell response to the O/core region of bacterial lipopolysaccharide is restricted to the Ly-1 lineage.
Experiments were performed to test the hypothesis that Ly-1 B cells respond to antigenic challenge with LPS from gram-negative bacteria. To perform these experiments, the splenic fragment culture system for the study of B cell precursors was used. We found that a significant number of anti-O/core, but not anti-lipid A, precursors expressed the lambda L chain. A restriction of the anti-LPS response to Ly-1 B cells was tested using a Ly-1 depletion protocol. We found that the anti-O/core antibody response was restricted to the Ly-1 B cell lineage. In contrast, conventional B cells, not Ly-1 B cells, respond to an antigenic challenge with lipid A. Our results further support the idea that the Ly-1 B cell lineage serves a direct role in protecting against certain bacterial infections.
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Department of Molecular Immunology, Roswell Park Memorial Institute (a unit of New York State Department of Health), Buffalo 14263.
Ward
M M
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Apicella
M A
MA
Ward
R E
RE
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D000950:Antigens, Ly / Q000032:analysis*; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007132:Immunoglobulin Isotypes / Q000276:immunology; D008050:Lipid A / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D019081:O Antigens; D012475:Salmonella / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000950:Antigens, Ly; D007132:Immunoglobulin Isotypes; D008050:Lipid A; D008070:Lipopolysaccharides; D019081:O Antigens