Vimentin and desmin expression in degenerating and regenerating dystrophic murine muscles.
The distribution of the intermediate filament proteins (IFP) desmin and vimentin was studied in gastrocnemius, plantaris and soleus muscles of the dystrophic mouse strain ReJ 129 during postnatal development. Special attention was paid to the overall morphological changes in the distribution of these cytoskeletal constituents in degenerating and regenerating muscle fibres. In contrast to their normal counterparts, the dystrophic mice (ReJ 129 dy/dy) appeared to develop four types of distinct muscle fibres with immunohistochemically detectable aberrant IFP patterns. The distribution of desmin IFP differed in the dystrophic muscle fibres as compared to the normal fibres in that juxtanuclear aggregates of IFP were frequently seen. In contrast to the recent literature we conclude that these cells are regenerated myofibres exhibiting defective nuclear migration.
Brocks
L
L
Department of Cell Biology and Histology, Faculty of Medical Sciences, University of Nijmegen, The Netherlands.
Jap
P H
PH
Ramaekers
F C
FC
Stadhouders
A M
AM
D000367:Age Factors; D000818:Animals; D003893:Desmin / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D009132:Muscles / Q000378:metabolism*; D009137:Muscular Dystrophy, Animal / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism*
D003893:Desmin; D014746:Vimentin