Physical and genetic mapping of a novel chromosome 19 ERCC1 marker showing close linkage with myotonic dystrophy.
Recent genetic linkage analyses have mapped the myotonic dystrophy locus to the region of 19q13.2-13.3 lying distal to the gene for creatine kinase subunit M (CKM). The human excision repair gene ERCC1 has also been mapped to this region of chromosome 19. A novel polymorphic DNA marker, pEO.8, has been isolated from a chromosome 19 ERCC1-containing cosmid that maps to a 300-kb NotI fragment encompassing both CKM and ERCC1. Genetic linkage analysis reveals close linkage between pEO.8 and myotonic dystrophy (DM) (zmax = 19.3, theta max = 0.01). Analysis of two key recombinant events suggests a mapping of DM distal to pEO.8 and CKM.
Division of Genetic, Children's Hospital of Eastern Ontario, Ottawa, Canada.
G
G
Shutler
A E
AE
MacKenzie
H
H
Brunner
B
B
Wieringa
P
P
de Jong
F P
FP
Lohman
S
S
Leblond
J
J
Bailly
R G
RG
Korneluk
D015139:Blotting, Southern; D002170:Canada; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D003402:Creatine Kinase / Q000235:genetics; D015342:DNA Probes; D004260:DNA Repair / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D009223:Myotonic Dystrophy / Q000235:genetics*; D009426:Netherlands; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic
D015342:DNA Probes; D005819:Genetic Markers; D003402:Creatine Kinase