Generation and characterization of infectious chimeric clones between human immunodeficiency virus type 1 and simian immunodeficiency virus from an African green monkey.
A series of chimeric clones of human immunodeficiency virus type 1 and simian immunodeficiency virus isolated from an African green monkey was constructed in vitro. In transient transfection experiments, all clones produced virion-associated reverse transcriptase, gag proteins, and env proteins. Eight out of 10 chimeric viruses clearly grew in the human CD4+ cell line C8166. Susceptibility of other CD4+ cell lines, MT-4, A3.01, and Molt4 clone 8, to infection with these viruses was also demonstrated.
Shibata
R
R
Department of Viral Oncology, Kyoto University, Japan.
Sakai
H
H
Kiyomasu
T
T
Ishimoto
A
A
Hayami
M
M
Adachi
A
A
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis; D002460:Cell Line; D002678:Chimera; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D003110:Colonic Neoplasms; D004279:DNA, Viral / Q000235:genetics; D015683:Gene Products, gag / Q000235:genetics; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000302:isolation & purification; D015497:HIV-1 / Q000235:genetics* / Q000254:growth & development; D006801:Humans; D007700:Kinetics; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000254:growth & development; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics
D015704:CD4 Antigens; D004279:DNA, Viral; D015683:Gene Products, gag; D015699:HIV Envelope Protein gp120; D014759:Viral Envelope Proteins; D012194:RNA-Directed DNA Polymerase