Inhibition of endotoxin-induced monocytic procoagulant activity by n-alcohols and anesthetics.
1. The effect of n-alcohols (methanol and ethanol) and anesthetics (lidocaine, thiopental, methohexital and thiamylal) on procoagulant activity (PCA) in human peripheral-blood monocytes and non-adherent cultured leukemia promonocytic U937 and THP-1 cells was examined herein. 2. Exposure of whole blood to ethanol showed no effect on PCA in human monocytes. However, ethanol dose-dependently inhibited LPS-induced PCA in isolated human monocytes. 3. In THP-1 cells, ethanol had no significant effect on PCA in either non-challenged or LPS-induced status. However, the induction of PCA by LPS was substantially inhibited when cells were pretreated with 1% ethanol (v/v) for 72 hr. 4. In U937 cells, n-alcohols and anesthetics resulted in dose-dependent depressions in PCA. Importantly, the percent reduction in LPS-induced PCA was much more pronounced than that in non-challenged PCA. 5. These data clearly suggest that n-alcohols and anesthetics readily inhibit the LPS-stimulatory action on monocytic PCA.
Research Division, Miami Heart Institute, Miami Beach, FL 31140.
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D000777:Anesthetics / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion; D002478:Cells, Cultured; D004731:Endotoxins / Q000037:antagonists & inhibitors*; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D007942:Leukemia, Experimental / Q000473:pathology; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008297:Male; D000432:Methanol / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D000777:Anesthetics; D004731:Endotoxins; D008070:Lipopolysaccharides; D000431:Ethanol; D000432:Methanol