Cloning of a promoter used by sigma H RNA polymerase in Bacillus subtilis.
The secondary RNA polymerase sigma factor sigma H is essential for endospore development in Bacillus subtilis. However, only a few promoters that are used by RNA polymerase containing sigma H (E sigma H) have been identified. We used in vitro transcription of random cloned fragments of B. subtilis chromosomal DNA to identify a promoter that is used by E sigma H. This promoter is active before the onset of sporulation.
Carter
H L
HL
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322.
Tatti
K M
KM
Moran
C P
CP
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics*; D014158:Transcription, Genetic
D012808:Sigma Factor; D012313:RNA