Two promoters control the aroH gene of Escherichia coli.
The aroH gene from Escherichia coli encodes 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase (Trp), one of three isoenzymes which catalyse the first committed step in the biosynthesis of aromatic amino acids and vitamins. S1 mapping and primer extension analysis of in vivo transcripts revealed the presence of two nonoverlapping promoters for aroH. The more distal of these has been described previously and is negatively regulated by the trp repressor. The second promoter is active under conditions of growth in rich medium, and may be involved in ensuring sufficient levels of precursors for the biosynthesis of aromatic vitamins under these growth conditions.
Research School of Biological Sciences, Australian National University, Canberra, A.C.T.
G S
GS
Hudson
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P
Rellos
B E
BE
Davidson
D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D007527:Isoenzymes; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012097:Repressor Proteins / Q000502:physiology; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D029968:Escherichia coli Proteins
D010723:3-Deoxy-7-Phosphoheptulonate Synthase; D001426:Bacterial Proteins; D007527:Isoenzymes; D012097:Repressor Proteins; D015719:Single-Strand Specific DNA and RNA Endonucleases; C027190:TRPR protein, E coli; D029968:Escherichia coli Proteins