Basophil histamine release by platelet-activating factor in aspirin-sensitive subjects with asthma.
Histamine release induced by platelet-activating factor (PAF) from leukocytes of aspirin-sensitive subjects with asthma was higher than that from normal control subjects, despite the similarity of anti-IgE-induced histamine release. Moreover, basophils of some aspirin-sensitive subjects with asthma released histamine by PAF stimulation in the absence of cytochalasin B that affects histamine release and is required in PAF-induced histamine release from leukocytes of atopic subjects with asthma and normal control subjects. In addition to temperature dependency and inhibition by ethylenediaminetetraacetic acid reported previously, PAF-induced histamine release was enhanced by cytochalasin B and indomethacin and inhibited by dexamethasone. These features are common with IgE-mediated histamine release and suggest the existence of the common pathway to PAF-induced histamine release and IgE-mediated histamine release. The results in the present study indicate the pathophysiologic significance of PAF-induced histamine release and that activation of basophils by PAF may be relevant to the pathogenesis in some aspirin-sensitive subjects with asthma.
Osaka Prefectural Habikino Hospital, Japan.
Y
Y
Okuda
H
H
Hattori
T
T
Takashima
A
A
Miyatake
A
A
Yamatodani
I
I
Tsuyuguchi
S
S
Kishimoto
D000328:Adult; D000368:Aged; D001241:Aspirin / Q000009:adverse effects*; D001249:Asthma / Q000139:chemically induced / Q000276:immunology*; D001491:Basophils / Q000187:drug effects* / Q000276:immunology; D003571:Cytochalasin B / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D006636:Histamine Release / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D010972:Platelet Activating Factor / Q000494:pharmacology*
D010972:Platelet Activating Factor; D007073:Immunoglobulin E; D003571:Cytochalasin B; D003907:Dexamethasone; D001241:Aspirin; D007213:Indomethacin