Regulation of histone H5 and H1 zero gene expression under the control of vaccinia virus-specific sequences in interferon-treated chick embryo fibroblasts.
The duck histone H5 and human H1 zero were inserted into the thymidine kinase (TK) gene of vaccinia virus and the interferon sensitivity of their expression under the control of the viral TK and P7.5 promoters in chick embryo fibroblasts (CEF) was compared to the interferon sensitivity of vaccinia virus WR specific TK induction. Expression and transport of these histones to the nucleus in CEF infected with the appropriate vaccinia virus recombinants could be detected with antisera raised against chick histone H5. In CEF cultivated for 3 days, interferon treatment that completely inhibited TK synthesis had no or only a marginal inhibitory effect on the expression of the histone genes. Inhibition of the expression of the histones could be detected under conditions of increased interferon sensitivity in aged CEF. The magnitude of inhibition was, however, less pronounced than the inhibition of viral TK synthesis. These data indicate that flanking vaccinia virus DNA regions confer interferon sensitivity to the expression of these histone genes, but that they contain structural information that partially exempts their expression from the inhibitory activity of the interferon-induced regulatory system.
Grün
J
J
Institute of Virology and Immunobiology, University of Würzburg, Federal Republic of Germany.
Redmann-Müller
I
I
Blum
D
D
Degen
H J
HJ
Doenecke
D
D
Zentgraf
H W
HW
Jungwirth
C
C
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004251:DNA Transposable Elements; D003851:Deoxyribonucleases / Q000378:metabolism; D004372:Ducks; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006657:Histones / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D007372:Interferons / Q000494:pharmacology*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013937:Thymidine Kinase / Q000235:genetics / Q000378:metabolism; D014616:Vaccinia virus / Q000187:drug effects / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics
D004251:DNA Transposable Elements; D006657:Histones; D015678:Viral Structural Proteins; D007372:Interferons; D013937:Thymidine Kinase; D003851:Deoxyribonucleases