Some monoclonal antibody reagents (C219 and JSB-1) to P-glycoprotein contain antibodies to blood group A carbohydrate determinants: a problem of quality control for immunohistochemical analysis.
Monoclonal antibodies (MAb) C219 and JSB-1 have been used extensively in the analysis of P-glycoprotein expression in normal and malignant tissues. This study demonstrates that some commercial lots of these MAb, even those supplied as purified immunoglobulins, contain contaminating anti-A blood group antibodies. In both sources of reagent, the antibody was specific for a particular A structure, known as repetitive or Type 3 A. These observations may account for earlier studies showing polymorphic variation in P-glycoprotein expression in epithelial tissues and an apparent correlation with the A blood type of the donor. Such reactivity can be eliminated by absorption of anti-P-glycoprotein reagents with A erythrocytes. These data re-emphasize the importance of evaluating MAb samples for unsuspected contaminating antibodies.
Finstad
C L
CL
Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Yin
B W
BW
Gordon
C M
CM
Federici
M G
MG
Welt
S
S
Lloyd
K O
KO
D000017:ABO Blood-Group System / Q000276:immunology*; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000276:immunology*; D002460:Cell Line; D002855:Chromatography, Thin Layer; D004912:Erythrocytes / Q000378:metabolism; D005187:Fallopian Tubes / Q000378:metabolism; D005260:Female; D006017:Glycolipids / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007150:Immunohistochemistry; D007518:Isoantibodies / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D011786:Quality Control; D013552:Swine
D000017:ABO Blood-Group System; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D006017:Glycolipids; D007518:Isoantibodies; D007519:Isoantigens; D008562:Membrane Glycoproteins