Cytokine-induced release of endothelin-1 from porcine renal epithelial cell line.
To elucidate the mechanism by which endothelin-1 (ET-1) is released from renal epithelial cells, we have investigated the effects of several compounds on release of ET-1-like immunoreactivity (LI) from LLCPK1 cell line. Thrombin, transforming growth factor-beta, cytokines (tumor necrotizing factor-alpha, interleukin-1 beta), and phorbol ester stimulated ET-1-LI release in a time- and dose-dependent manner. The cytokine-induced ET-1-LI release was not affected by indomethacin. Northern blot analysis using cDNA for porcine preproET-1 as a probe revealed a single major band corresponding to the size of ET-1 mRNA in LLCPK1. These data indicate that the preproET-1 gene is also expressed in renal epithelial cells and the release of ET-1 from renal cells is regulated by the similar mechanism to that from endothelial cells.
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
K
K
Ohta
Y
Y
Hirata
T
T
Imai
K
K
Kanno
T
T
Emori
M
M
Shichiri
F
F
Marumo
D000818:Animals; D001685:Biological Factors / Q000494:pharmacology*; D015152:Blotting, Northern; D002460:Cell Line; D016207:Cytokines; D016232:Endothelins; D004730:Endothelium, Vascular; D004848:Epithelium; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D007668:Kidney; D007700:Kinetics; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology; D015291:Transforming Growth Factors / Q000494:pharmacology
D001685:Biological Factors; D016207:Cytokines; D016232:Endothelins; D007375:Interleukin-1; D010455:Peptides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D011061:Poly A; D012313:RNA; D015291:Transforming Growth Factors; D013917:Thrombin; D013755:Tetradecanoylphorbol Acetate; D007213:Indomethacin