Acidic fibroblast growth factor (HBGF-1) stimulates DNA synthesis in primary rat hepatocyte cultures.
Acidic fibroblast growth factor (aFGF) stimulated DNA synthesis in primary rat hepatocyte cultures in a dose-dependent manner with maximal effect at 10-50 ng ml-1. This activity was dependent on the presence of heparin at a concentration of 10-50 micrograms.ml-1. Insulin interacted synergistically with aFGF, as it did with epidermal growth factor (EGF). The response to aFGF was only 50% that found with EGF. The disparity was not due to different kinetics of DNA synthesis, since the peak response for both growth factors occurred at 36-72 hr after plating of the hepatocytes. The potential relevance of this novel hepatocyte mitogen to normal and pathological liver growth is discussed.
Houck
K A
KA
Department of Pathology, Duke University Medical Center, Durham, North Carolina.
Zarnegar
R
R
Muga
S J
SJ
Michalopoulos
G K
GK
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016220:Fibroblast Growth Factor 1; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D011994:Recombinant Proteins; D013997:Time Factors
D006133:Growth Substances; D007328:Insulin; D011994:Recombinant Proteins; D016220:Fibroblast Growth Factor 1; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D006493:Heparin; D004247:DNA