Expression of the myeloid differentiation antigen CD33 depends on the presence of human chromosome 19 in human-mouse hybrids.
Interlineage human-mouse hybrids were constructed by fusion of human acute undifferentiated leukaemia cells with the mouse thymoma cell line BW5147. Some of the hybrids expressed the human differentiation antigens CD4, CD7, CD33, and CD71 (transferrin receptor). Chromosome analysis revealed that the expression of the myeloid antigen CD33 is dependent on the presence of human chromosome 19, which is in agreement with the location of CD33-coding sequences on chromosome 19, as recently reported by Peiper et al. (1987). Furthermore, these hybrids allowed us to confirm the assignment of the CD4 antigen, the CD7 antigen, and the CD71 antigen to human chromosomes 12, 17 and 3, respectively.
Department of Immunology, University Hospital Dijkzigt/Erasmus University, Rotterdam, The Netherlands.
H J
HJ
Adriaansen
A H
AH
Guerts van Kessel
J H
JH
Wijdenes-de Bresser
E
E
van Drunen-Schoenmaker
J J
JJ
van Dongen
D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics*; D019011:Antigens, CD7; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis / Q000235:genetics*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000235:genetics; D015704:CD4 Antigens / Q000096:biosynthesis / Q000235:genetics; D002871:Chromosome Banding; D002888:Chromosomes, Human, Pair 19; D006801:Humans; D006822:Hybrid Cells; D007938:Leukemia / Q000235:genetics*; D008297:Male; D051379:Mice; D011990:Receptors, Transferrin / Q000096:biosynthesis / Q000235:genetics; D063268:Sialic Acid Binding Ig-like Lectin 3; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D019011:Antigens, CD7; D015214:Antigens, Differentiation, Myelomonocytic; D000945:Antigens, Differentiation, T-Lymphocyte; C571163:CD33 protein, human; D015704:CD4 Antigens; C571165:Cd33 protein, mouse; D011990:Receptors, Transferrin; D063268:Sialic Acid Binding Ig-like Lectin 3