Serum beta-hexosaminidase and alpha-mannosidase activities as markers of alcohol abuse.
In 25-alcohol-dependent patients in whom detoxication treatment has been introduced serum total beta-hexosaminidase, thermostable beta-hexosaminidase, alpha-mannosidase and gamma glutamylotransferase (GGT) and serum high density lipoprotein (HDL) cholesterol were determined during alcohol withdrawal. The assays were also performed in a group of dependent individuals after a 1 month or longer period of abstinence. Marked increase in beta-hexosaminidase activity was observed in intoxicated patients. The activity decreased rapidly after the cessation of drinking, resembling the decrease in HDL cholesterol level in its dynamics. The alpha-mannosidase activity rise was less pronounced and its normalization was slow, similar to the GGT activity normalization rate. The rise of beta-hexosaminidase activity was mostly due to the thermostable component of the enzyme. Total beta-hexosaminidase or thermostable beta-hexosaminidase activity determinations appear to be simple and reliable markers of alcohol abuse.
Department of Genetics, Institute of Psychiatry and Neurology, Warsaw, Poland.
H
H
Wehr
B
B
Czartoryska
D
D
Górska
H
H
Matsumoto
D000328:Adult; D000437:Alcoholism / Q000175:diagnosis* / Q000201:enzymology / Q000534:rehabilitation; D008076:Cholesterol, HDL / Q000097:blood; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008361:Mannosidases / Q000097:blood*; D008875:Middle Aged; D012016:Reference Values; D043323:alpha-Mannosidase; D001619:beta-N-Acetylhexosaminidases / Q000097:blood*; D005723:gamma-Glutamyltransferase / Q000097:blood
D008076:Cholesterol, HDL; D005723:gamma-Glutamyltransferase; D008361:Mannosidases; D043323:alpha-Mannosidase; D001619:beta-N-Acetylhexosaminidases