Leuhistin, a new inhibitor of aminopeptidase M, produced by Bacillus laterosporus BMI156-14F1. I. Taxonomy, production, isolation, physico-chemical properties and biological activities.
Leuhistin has been isolated from the culture broth of Bacillus laterosporus BMI156-14F1 as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M (AP-M). It was purified by use of column chromatography on Sepabeads SP206, Amberlite IRC-50, MCI gel CHP-20P and Sephadex G-10 and then isolated as colorless needles. Leuhistin inhibits AP-M strongly and it also inhibits AP-A and AP-B weakly. It is competitive with the substrate, and the inhibition constant (Ki) was 2.3 x 10(-7) M.
Aoyagi
T
T
Institute of Microbial Chemistry, Tokyo, Japan.
Yoshida
S
S
Matsuda
N
N
Ikeda
T
T
Hamada
M
M
Takeuchi
T
T
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000626:Aminopeptidases / Q000037:antagonists & inhibitors*; D000818:Animals; D001407:Bacillus / Q000145:classification / Q000378:metabolism*; D018826:CD13 Antigens; D007093:Imidazoles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D009842:Oligopeptides / Q000494:pharmacology; D012995:Solubility
D000596:Amino Acids; D007093:Imidazoles; D009842:Oligopeptides; C063432:probestin; C069912:leuhistin; D000626:Aminopeptidases; D018826:CD13 Antigens