Induction of chromosomal aberrations by DNase I.
Chinese hamster ovary (CHO) cells were treated with bovine pancreatic DNase I using the method of electroporation. The enzyme induced chromosomal aberrations in a S-phase independent manner. The frequencies of polycentric chromosomes induced in the G1 phase of the cell cycle are positively correlated with the dose of DNase I. The distributions of DNase I-induced polycentric chromosomes were overdispersed.
Division of Human Cytogenetics and Quantitative Microscopy, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
G A
GA
Folle
C
C
Johannes
G
G
Obe
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D003850:Deoxyribonuclease I / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016193:G1 Phase / Q000502:physiology; D010179:Pancreas / Q000201:enzymology; D016196:S Phase / Q000502:physiology
D003850:Deoxyribonuclease I