DNA binding activity of the BarH1 homeodomain of Drosophila.
Using a series of deletion mutants of BarH1, a Drosophila homeobox gene required for eye morphogenesis, the DNA-binding region of the BarH1 protein was determined. Not only homeodomain but also its upstream sequence were found to be necessary for binding, whereas about a half of the conserved downstream sequence (Bar domain) was dispensable.
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
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Saigo
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D005136:Eye Proteins / Q000378:metabolism*; D005801:Genes, Homeobox; D018398:Homeodomain Proteins; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000378:metabolism*
C072318:B-H1 protein, Drosophila; D029721:Drosophila Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D014157:Transcription Factors; D004247:DNA