A cystic fibrosis pancreatic adenocarcinoma cell line.
We established a pancreatic adenocarcinoma cell line (CFPAC-1) from a patient with cystic fibrosis (CF) and assessed some of its properties. The cells show epithelial morphology and express cytokeratin and oncofetal antigens characteristic of pancreatic duct cells. Basal and stimulated levels of cAMP and cAMP-dependent protein kinase and the biophysical properties of single Cl- channels in CFPAC-1 are similar to those of airway and sweat gland primary cultures and Cl(-)-secreting epithelial cell lines. Anion transport and single Cl- channel activity was stimulated by Ca2+ ionophores but not by forskolin, cAMP analogs, or phosphodiesterase inhibitors. The cells express the CF gene and manifest the most common CF mutation, deletion of three nucleotides resulting in a phenylalanine-508 deletion. These properties have been stable through greater than 80 passages (24 months), suggesting that CFPAC-1 can serve as a continuous cell line that displays the CF defect.
Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama, Birmingham 35294.
R A
RA
Schoumacher
J
J
Ram
M C
MC
Iannuzzi
N A
NA
Bradbury
R W
RW
Wallace
C T
CT
Hon
D R
DR
Kelly
S M
SM
Schmid
F B
FB
Gelder
T A
TA
Rado
D000230:Adenocarcinoma / Q000150:complications / Q000473:pathology*; D000951:Antigens, Neoplasm / Q000032:analysis; D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D018118:Chloride Channels; D002712:Chlorides / Q000502:physiology; D046508:Culture Techniques / Q000379:methods; D000242:Cyclic AMP / Q000032:analysis; D003550:Cystic Fibrosis / Q000150:complications / Q000473:pathology*; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D005091:Exons; D005784:Gene Amplification; D007473:Ion Channels / Q000502:physiology; D008565:Membrane Proteins / Q000502:physiology; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010190:Pancreatic Neoplasms / Q000150:complications / Q000473:pathology*; D011494:Protein Kinases / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure
D000951:Antigens, Neoplasm; D018118:Chloride Channels; D002712:Chlorides; D004273:DNA, Neoplasm; D007473:Ion Channels; D008565:Membrane Proteins; D015345:Oligonucleotide Probes; D012334:RNA, Neoplasm; D000242:Cyclic AMP; D011494:Protein Kinases