The route of lysine breakdown in Candida tropicalis.
Candida tropicalis was found to contain high levels of the following enzymes after growth in defined medium on L-lysine as sole nitrogen source: L-lysine N6-acetyltransferase, N6-acetyl-lysine aminotransferase, and aminotransferase activity for 5-aminovalerate and 4-aminobutyrate. Extracts were also capable of converting 5-acetamidovalerate (and 4-acetamidobutyrate) to acetate. N6-Acetyllysine however, only gave rise to acetate in the presence of 2-oxoglutarate, NAD+ and thiamine pyrophosphate. These activities were undetectable or present in much lower concentrations in cells that had been grown on ammonium sulphate as sole nitrogen source. It is concluded that L-lysine is degraded in this organism via N6-acetyllysine, 5-acetamidovalerate and 5-aminovalerate, both nitrogen atoms being removed by transamination.
Large
P J
PJ
Department of Applied Biology, University of Hull, U.K.
Robertson
A
A
D000085:Acetates / Q000032:analysis; D019342:Acetic Acid; D000123:Acetyltransferases / Q000378:metabolism; D002175:Candida / Q000201:enzymology / Q000378:metabolism*; D005971:Glutamates / Q000032:analysis; D018698:Glutamic Acid; D007700:Kinetics; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism*; D012248:Rhodotorula / Q000201:enzymology / Q000378:metabolism; D013696:Temperature; D000637:Transaminases / Q000378:metabolism
D000085:Acetates; D005971:Glutamates; D018698:Glutamic Acid; C016949:N-epsilon-acetyllysine; D000123:Acetyltransferases; D000637:Transaminases; D008239:Lysine; D019342:Acetic Acid