Detection of human hemoglobin A (HbA) and human hemoglobin F (HbF) in biological stains by microtiter latex agglutination-inhibition test.
This paper describes the detection of human HbA and human HbF antigens in bloodstains by an antibody-absorption test. In the test, antisera to human HbA or HbF were absorbed with biological stains, then the antibodies with decreased capacities for agglutination were titrated by a microtiter technique using latex particles coated with the corresponding antigens. This test was proved to be specific, sensitive and reproducible. The indicator latex particles coated with human HbA or HbF could be stored at -70 degrees C for over 10 years.
Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan.
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Itoh
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Matsuzawa
D000042:Absorption; D000818:Animals; D001793:Blood Preservation; D001801:Blood Stains; D005312:Fetal Blood / Q000737:chemistry*; D005319:Fetal Hemoglobin / Q000032:analysis*; D005404:Fixatives; D006441:Hemoglobin A / Q000032:analysis*; D006801:Humans; D007841:Latex Fixation Tests; D000432:Methanol; D011237:Predictive Value of Tests
D005404:Fixatives; D006441:Hemoglobin A; D005319:Fetal Hemoglobin; D000432:Methanol