Stimulation of the absorption of macromolecules in Giardia lamblia infected mice intestine.
The absorption of 125I-labelled bovine serum albumin, gamma-globulin and alpha-lactalbumin was considerably enhanced in G. lamblia infected Swiss mice intestine compared to uninfected controls. The binding of 125I-proteins to brush border membrane was however, significantly (P less than 0.01) low in infected animals. Kinetic studies with gamma-globulin binding to brush borders revealed a decrease in the number of binding sites in infected animals (1.52) compared to controls (2.86 micrograms/mg protein) with no change in the affinity constant (47.60 micrograms/ml) under these conditions. These findings suggest that G. lamblia infection in mice leads to enhanced macromolecular absorption which seems unrelated to the binding of proteins to epithelial cell surface.
Mahmood
S
S
Department of Experimental Medicine, Postgraduate Institute of Medical Education & Research, Chandigarh.
Ganguly
N K
NK
Mahajan
R C
RC
Walia
B N
BN
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005873:Giardiasis / Q000378:metabolism*; D007408:Intestinal Absorption; D007768:Lactalbumin / Q000378:metabolism*; D008297:Male; D051379:Mice; D008871:Microvilli / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D005719:gamma-Globulins / Q000378:metabolism*
D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D007768:Lactalbumin