Recovery of RNA from flow-sorted fixed cells.
We describe a method for the preparation of RNA from ethanol-fixed cells, allowing analysis of the RNA from cells "frozen" in a given physiological state. This technique may have important applications in experiments which require prolonged cell manipulations before RNA preparation, such as investigations of cell-cycle-regulated gene expression, which require the preparation of cells for cell-cycle flow analysis, and even for long-term cell sorting. It eliminates all the inconveniences associated with the use of fresh cells, and allows cell-cycle biologists to couple flow cytometry methodology with the advancing techniques of molecular biology.
Khochbin
S
S
Laboratoire de Biologie Moléculaire du Cycle Cellulaire, Unité INSERM 309, Grenoble, France.
Grunwald
D
D
Pabion
M
M
Lawrence
J J
JJ
D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D000431:Ethanol; D005404:Fixatives; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation; D012313:RNA / Q000302:isolation & purification*; D014407:Tumor Cells, Cultured
D005404:Fixatives; D000431:Ethanol; D012313:RNA