Hallucinogenic and neuroleptic drug interactions with potential neurotransmitter receptors in parasitic nematodes.
Receptors potentially involved in neurotransmitting have been characterised in the muscle tissue and in whole worms of the nematodes Ascaris suum and Onchocerca volvulus, respectively. Binding studies revealed a high affinity for LSD with apparent KD values of 94 nM for A. suum and 120 nM for O. volvulus, whereas those of the neuroleptics haloperidol, spiperone and mianserin were found to be in the micromolar range. A variety of neurotransmitter antagonists, known to bind with high affinities either to mammalian D1/2 or to 5-HT1/2 receptors, were tested for their ability to bind to the nematode receptor. Results from these displacement experiments using tritiated LSD, mianserin, spiperone and haloperidol show distinct specificities of the nematode receptors compared to known receptor classes of mammals. With respect to this novel specificity, the nematode receptors seem to be unique and clearly distinct from those of the hosts.
Department of Biochemistry, Bernhard Nocht Institute for Tropical Medicine, Hamburg, F.R.G.
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Albrecht
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Walter
D000818:Animals; D014150:Antipsychotic Agents / Q000378:metabolism*; D001200:Ascaris / Q000378:metabolism*; D001667:Binding, Competitive; D004048:Diethylamines / Q000378:metabolism; D006220:Haloperidol / Q000378:metabolism; D008238:Lysergic Acid Diethylamide / Q000378:metabolism*; D008803:Mianserin / Q000378:metabolism; D009854:Onchocerca / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013134:Spiperone / Q000378:metabolism
D014150:Antipsychotic Agents; D004048:Diethylamines; D017981:Receptors, Neurotransmitter; D008803:Mianserin; D013134:Spiperone; D008238:Lysergic Acid Diethylamide; D006220:Haloperidol