The thyroid stimulating hormone receptor in human disease.
The initial step in the action of thyrotropin (TSH) is its binding to the TSH receptor. TSH receptor antibodies are detected in up to 90% of patients with Graves' disease. Serial measurements of TSH receptor antibodies in patients with Graves' hyperthyroidism are helpful in predicting relapse. The TSH receptor was purified using affinity chromatography on wheat germ lectin agarose and TSH-agarose. Using an immunoblotting technique to characterize the TSH receptor, it was found to be an oligomeric glycoprotein consisting of three noncovalently bound subunits of Mr approximately 70,000, approximately 50,000 and approximately 35,000 which on reduction yield a single subunit of Mr approximately 25,000.
Burnet Clinical Research Unit, Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Parkville, Victoria, Australia.
L C
LC
Harrison
P J
PJ
Leedman
D000906:Antibodies / Q000302:isolation & purification*; D001666:Binding Sites, Antibody; D002846:Chromatography, Affinity; D000939:Epitopes / Q000032:analysis / Q000502:physiology; D006111:Graves Disease / Q000276:immunology; D006801:Humans; D006980:Hyperthyroidism / Q000276:immunology; D007037:Hypothyroidism / Q000276:immunology; D011989:Receptors, Thyrotropin / Q000276:immunology / Q000302:isolation & purification*; D013959:Thyroid Diseases / Q000276:immunology*
D000906:Antibodies; D000939:Epitopes; D011989:Receptors, Thyrotropin