The N- and C-terminal boundaries of myelin basic protein determinants required for encephalitogenic and proliferative responses of Lewis rat T cells.
Highly purified synthetic peptides representing portions of the 68-86 sequence of guinea pig (GP) myelin basic protein (GPMBP) were used to define the N- and C-termini of encephalitogenic determinants that cause experimental autoimmune encephalomyelitis (EAE) in Lewis rats. Each peptide was tested for: (a) induction of EAE, (b)in vitro potentiation of EAE transfer activity by GPMBP-sensitized lymph node cells (LNC), (c) in vitro proliferation of GPMBP-sensitized LNC, and (d) in vitro proliferation of a GPMBP-reactive line of EAE-inducing T cells. In these bioassays, the general rank order of potency was: GPMBP greater than or equal to GP68-86 greater than or equal to GP72-86 greater than [G84]GP68-86 greater than or equal to GP68-84 much greater than GP75-85 greater than or equal to GP75-84 = virtually no activity. These results demonstrate that the encephalitogenic region is bounded by the 72-74 and 84-86 sequences. Further evidence presented herein indicates that the 75-84 sequence contains the primary antigenic features required for specific T cell recognition of the encephalitogenic region.
Department of Microbiology-Immunology, Medical School, Northwestern University, Chicago, IL 60611.
M D
MD
Mannie
P Y
PY
Paterson
D C
DC
U'Prichard
G
G
Flouret
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D004660:Encephalitis / Q000276:immunology* / Q000473:pathology; D000939:Epitopes; D007116:Immunization, Passive; D007120:Immunochemistry; D008297:Male; D004676:Myelin Basic Protein / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000473:pathology / Q000502:physiology*
D000939:Epitopes; D004676:Myelin Basic Protein; D010455:Peptides