Amplification and differentiation of the DNA of an abortigenic (type 1) and a respiratory (type 4) strain of equine herpesvirus by the polymerase chain reaction.
Unpurified DNA derived from cultures of equine fetal kidney cells infected with either equine herpesvirus type 1 or equine herpesvirus type 4 was amplified by the polymerase chain reaction using one pair of oligonucleotide primers. Restriction endonuclease digestion of the amplified segments with PvuII, followed by electrophoresis, revealed restriction fragment length polymorphisms which enabled the two virus types to be differentiated.
O'Keefe
J S
JS
Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand.
Murray
A
A
Wilks
C R
CR
Moriarty
K M
KM
D000034:Abortion, Veterinary / Q000382:microbiology; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry; D005260:Female; D005784:Gene Amplification; D006564:Herpesviridae / Q000145:classification / Q000235:genetics*; D004861:Herpesvirus 1, Equid / Q000145:classification / Q000235:genetics*; D006734:Horse Diseases / Q000382:microbiology; D006736:Horses; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral