Loss of encephalitogenicity of a myelin basic protein-specific T cell line is associated with a phenotypic change but not with alteration in production of interleukin-2, gamma-interferon or tumour necrosis factor.
Continued stimulation of a CD4+ myelin basic protein-specific T cell line led to loss of in vivo encephalitogenic activity but no alteration in ability of the line to produce interleukin-2, gamma-interferon or tumour necrosis factor. Loss of encephalitogenicity was, however, associated with an increased presence of CD4+ CD8+ cells which likely represents preferential outgrowth of this population. The possible in vivo relevance of these findings is discussed.
MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, U.K.
M J
MJ
Day
D W
DW
Mason
D000818:Animals; D002460:Cell Line; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology*; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D008297:Male; D004676:Myelin Basic Protein / Q000276:immunology*; D010641:Phenotype; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D007376:Interleukin-2; D004676:Myelin Basic Protein; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma