Regulation by induction of branched-chain 2-oxo acid dehydrogenase complex in clofibrate-fed rat liver.
The activity of branched-chain 2-oxo acid dehydrogenase complex increased 3.0-fold in liver of rats fed on 0.1%(w/w) clofibrate. Immunotitration experiments with antibodies against the constituent enzymes of the complex revealed that this increase resulted mainly from the increased amounts of only two(a decarboxylase and a lipoate acyltransferase) of three components of the complex and that the other component(dihydrolipoamide dehydrogenase) remained unchanged in its content, irrespective of clofibrate administration. The increases of both enzyme components were associated with increases in their mRNA levels which were estimated by in vitro translation with poly(A)+ RNA.
Department of Biochemistry, Fukushima Medical College, Japan.
K
K
Ono
H
H
Shioya
M
M
Hakozaki
K
K
Honda
T
T
Mori
H
H
Kochi
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000818:Animals; D002994:Clofibrate / Q000494:pharmacology*; D007658:Ketone Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000235:genetics; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D009097:Multienzyme Complexes; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D002994:Clofibrate