Genetic analysis of amylase-producing cell lines: ectopic activation of the amylase gene by translocation.
Two amylase-producing cell lines have been established, KMK-2 from a patient with gastric cancer, and KHM-1B from a patient with IgA lambda-type multiple myeloma. Both patients exhibited extremely high levels of serum amylase. The production of S-type amylase m-RNA by KMK-2 and KHM-1B was demonstrated by Northern blot analysis. Chromosome analysis showed many qualitative and quantitative abnormalities in both cell lines. In KHM-1B, a translocation was found between 1p13 or 21, near the amylase genes locus, and 9q34, the abl oncogene locus. These findings suggest the amylase gene in KHM-1B to be activated by translocation. A rearranged amylase gene was demonstrated by Southern blot analysis with only one enzyme, Accl.
Second Department of Internal Medicine, Kumamoto University Medical School.
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Hata
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Matsuzaki
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Sanada
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Takatsuki
D000681:Amylases / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007621:Karyotyping; D009101:Multiple Myeloma / Q000201:enzymology* / Q000235:genetics*; D012334:RNA, Neoplasm / Q000032:analysis; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics*; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured / Q000201:enzymology
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D000681:Amylases