In vitro assembly of poliovirus 14 S subunits: disoxaril stabilization as a model for the antigenicity conferring activity of infected cell extracts.
14 S subunits of poliovirus at 37 degrees undergo a rapid thermodenaturation, whereby all neutralizing epitopes are lost. This denaturation is prevented by 1 microgram/ml disoxaril, up to 42 degrees. In the absence of disoxaril, self-assembly of 14 S subunits at 37 degrees yields H-antigenic empty capsids. However, in the presence of disoxaril, and provided the molar ratio of disoxaril per 14 S subunit exceeds 7, the empty capsids generated by self-assembly are N-antigenic, and indistinguishable from native procapsids extracted from infected cells.
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
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Rombaut
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Boeyé
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D000998:Antiviral Agents / Q000494:pharmacology*; D002213:Capsid / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000032:analysis; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007555:Isoxazoles / Q000494:pharmacology*; D007700:Kinetics; D017955:Poliovirus / Q000187:drug effects / Q000502:physiology*; D013696:Temperature
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000998:Antiviral Agents; D000939:Epitopes; D007555:Isoxazoles; C045978:disoxaril