Synthetic peptide segments from the Escherichia coli porin OmpF constitute leukocyte activators.
After characterization of the porin OmpF and selection of molecular structures responsible for leukocyte activation by using computer-assisted epitope analysis, the analogs OmpF (153-174) (containing amino acids 153 to 174), OmpF (157-174), and OmpF (275-285) were synthesized and tested. Like the native protein, the segments were mitogenic for BALB/c splenocytes and induced B lymphocyte differentiation into antibody-producing plasma cells and tumor cytotoxicity of macrophages against the fibroblast cell line L929. We thus demonstrated that defined peptide segments are responsible for the leukocyte-activating properties of a major bacterial surface protein.
Institut für Immunobiologie der Universität, Freiburg, Federal Republic of Germany.
H M
HM
Vordermeier
P
P
Hoffmann
F O
FO
Gombert
G
G
Jung
W G
WG
Bessler
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000138:chemical synthesis / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D000939:Epitopes; D004926:Escherichia coli / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D018272:Porins; D013154:Spleen / Q000276:immunology; D014407:Tumor Cells, Cultured
D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D010455:Peptides; D018272:Porins