Seminal plasma protein p30: simplified purification and evidence for identity with prostate specific antigen.
A rapid high yield purification scheme is described for the major 30,000 molecular weight glycoprotein (p30) in human seminal plasma. Immunological and protein sequence evidence demonstrates the identity of this protein with prostate specific antigen (PSA) and gamma-seminoprotein (gamma SM).
Sensabaugh
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Forensic Science Group, School of Public Health, University of California, Berkeley 94720.
Blake
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D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D015153:Blotting, Western; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D017430:Prostate-Specific Antigen; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000302:isolation & purification*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D017430:Prostate-Specific Antigen