Effect of low doses of a trichothecene mycotoxin (diacetoxyscirpenol) on rat gastric glycoproteins: a histochemical study.
A histochemical study was carried out to evaluate the changes occurring in the glycoproteins of the stomach of the rat following short-term treatment with the trichothecene mycotoxin, diacetoxyscirpenol (DAS). Staining with alcian blue methods for detecting complex carbohydrates and with lectin conjugates (Con A, LTA, PNA, SBA and WGA) showed an increased alcianophilia at pH 2.6 and pH 1.0 for various parts of the fundic glands. With respect to lectin staining, DAS intoxication was characterized by enhanced labelling with LTA and SBA in the surface epithelium and in the foveolae, while WGA binding appeared in the lower mucous neck cells. These data suggest that the contents of the mucus-producing cells of the fundic glands of the rat stomach could be affected by low doses of diacetoxyscirpenol even following only 2 days of treatment.
Laboratoire de Pharmacologie-Toxicologie, INRA, Toulouse, France.
J
J
Moré
P
P
Galtier
C
C
Eeckhoutte
D000818:Animals; D001665:Binding Sites; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006651:Histocytochemistry; D008297:Male; D009183:Mycotoxins / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012717:Sesquiterpenes / Q000633:toxicity*; D013194:Staining and Labeling; D014255:Trichothecenes / Q000633:toxicity*
D006023:Glycoproteins; D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes; C001809:diacetoxyscirpenol