Adsorption of anti-dsDNA antibodies by immobilized polyanionic compounds.
Antibodies to dsDNA are characteristically present in serum from patients with systemic lupus erythematosus (SLE), and have been shown to have the capacity to react with various molecules bearing repeating negative charges. After a number of polymeric or monomeric molecules with differently charged groups and hydrophobic molecules had been coupled covalently as ligands on cellulose gel, the adsorption capacities of the ligands for anti-dsDNA antibodies were evaluated. It was found that gels coupled with polyanionic dextran sulphate (DXS) and polyacrylic acid (PA) and monoanionic sulphanilic acid (SA) absorbed anti-dsDNA antibodies effectively. DXS gel also adsorbed antibodies to ssDNA and heparan sulphate, antigens with repeating negatively charged moieties, while no ligand was able to adsorb anti-nRNP antibodies. The finding that DXS gels adsorbed anti-dsDNA antibody in proportion to their charge density, and that the interaction between anti-dsDNA and DXS gel is broken readily by an increase in ionic strength, indicated that the binding is ionic in nature. Moreover, virtually all F(ab')2 anti-dsDNA became adsorbed onto the DXS gels, suggesting that the binding occurred via specific antigen-binding sites on the antibody molecule. Binding of these polyanion-binding autoantibodies with anionic sites in the glomerular basement membrane may therefore cause the tissue damage observed in SLE.
Division of Immunology, National Medical Centre, Tokyo, Japan.
S
S
Aotsuka
T
T
Funahashi
N
N
Tani
M
M
Okawa-Takatsuji
M
M
Kinoshita
R
R
Yokohari
D000327:Adsorption; D000974:Antibodies, Antinuclear / Q000378:metabolism*; D016264:Dextran Sulfate; D003911:Dextrans / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D009994:Osmolar Concentration
D000974:Antibodies, Antinuclear; D003911:Dextrans; D007140:Immunoglobulin Fab Fragments; D016264:Dextran Sulfate