The Ca2+ antagonists binding cytosolic protein has properties of the Ca2+ channel.
In our previous work (Krizanová et al. 1989) we have described a protein from rabbit skeletal muscle cytosolic fraction, which is able to bind dihydropyridines and phenothiazines. In the present work conclusive evidence is provided for the ability of the phospholipid-reconstituted cytosolic protein to transport calcium. The calcium transport was stimulated by BAY K 8644 and inhibited in the presence of PN 200-110. Our observations were confirmed also by electrophysiological measurements on planar lipid bilayers. The possibility that the cytosolic fraction was contaminated with membranes could be definitely ruled out. Nevertheless, the nature of the protein under study is still in the frame of guess.
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
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Krizanová
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Hurnák
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Hudecová
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D003600:Cytosol / Q000502:physiology; D008051:Lipid Bilayers; D008564:Membrane Potentials / Q000187:drug effects; D008970:Molecular Weight; D009132:Muscles / Q000502:physiology*; D011510:Proteolipids / Q000378:metabolism; D011817:Rabbits; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*
D015220:Calcium Channels; D008051:Lipid Bilayers; D011510:Proteolipids; D011978:Receptors, Nicotinic; C020485:proteoliposomes; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D002118:Calcium