Nisin biosynthesis genes are encoded by a novel conjugative transposon.
Genes for biosynthesis of the lactococcal peptide antibiotic nisin were shown to be encoded by a novel chromosomally located transposon Tn5301. The element is 70 kb in size and lacks inverted repeats at its termini. Although a copy of the insertion sequence IS904 is located near to one end, this did not appear to be involved in the transposition process. The integrated element is flanked by the directly repeated sequence 5'-TTTTTG-3'. Analysis of ten independent transconjugants revealed that Tn5301 integration is site-specific; two chromosomal targets were identified and shown to have some sequence homology. The element shares features with the Tn916 family of conjugative transposons and with Tn554 but is also exhibits some unique properties. Tn5301 is thus considered to be the prototype of a novel class of conjugative transposon.
AFRC Institute of Food Research, Norwich Laboratory, UK.
N
N
Horn
S
S
Swindell
H
H
Dodd
M
M
Gasson
D001483:Base Sequence; D015139:Blotting, Southern; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D005798:Genes, Bacterial; D007934:Leuconostoc / Q000235:genetics*; D008969:Molecular Sequence Data; D009561:Nisin / Q000096:biosynthesis / Q000235:genetics*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004251:DNA Transposable Elements; D009561:Nisin