In vitro murine lymphocyte blastogenic responses to Cryptosporidium parvum.
Spleen and mesenteric lymph node lymphocytes from both Cryptosporidium parvum-exposed and unexposed mice were cultured with antigen (Ag) prepared from C. parvum oocysts. Spleen lymphocytes from oral-, intraperitoneal-, or oral + intraperitoneal-exposed mice did not respond significantly (P greater than 0.05) to Ag stimulation. Spleen lymphocytes from multioral-exposed mice, however, demonstrated significant (P less than or equal to 0.01) Ag-specific blastogenesis. Mesenteric lymph node lymphocytes did not respond to in vitro Ag stimulation regardless of the route of in vivo priming. These results demonstrate an in vitro cell-mediated immune response against C. parvum by lymphocytes in murine spleen.
USDA, National Animal Disease Center, Ames, Iowa 50010.
W M
WM
Whitmire
J A
JA
Harp
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D002478:Cells, Cultured; D003044:Coccidia / Q000276:immunology*; D003457:Cryptosporidiosis / Q000276:immunology; D003458:Cryptosporidium / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008297:Male; D008643:Mesentery; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology
D000953:Antigens, Protozoan; D000939:Epitopes