[Cytokeratin expression in squamous-cell metaplasia of the cervix uteri: an immunohistochemical study using monoclonal antibodies].
Immunohistochemical study of the exo- and endocervix in squamous metaplasia is performed using EE21-06d monoclonal antibodies revealing simultaneously five cytokeratinous polypeptides characteristic of squamous epithelium, and f-12-19h revealing simultaneously wide spectrum of cytokeratinous polypeptides characteristic of the epithelium various types. PAP method and the reaction of immunofluorescence were used. Monoclonal antibodies 12-19d label cells of all layers of normal exocervix, endocervix and metaplastic squamous epithelium, both immature and differentiated. Monoclonal antibodies EE21-06d react in the exocervix with suprabasal and higher located cells and in the endocervix with reserve cells only. Positive reaction with EE21-06d in the metaplastic foci increases in proportion with the squamous cell differentiation, thus this antibody being the marker of reserve cells and the degree of the squamous metaplasia maturity.
N T
NT
Raĭkhlin
V A
VA
Dobrynin
S V
SV
Petrov
G
G
Serre
D000328:Adult; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001706:Biopsy; D002584:Cervix Uteri / Q000378:metabolism* / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007633:Keratins / Q000276:immunology / Q000378:metabolism*; D008679:Metaplasia / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D007633:Keratins
Ekspressiia tsitokeratinov pri ploskokletochnoĭ metaplazii sheĭki matki: immunogistokhimicheskoe issledovanie s pomoshch'iu monoklonal'nykh antitel.