Phosphotyrosine-containing proteins in Dictyostelium discoideum.
Phosphotyrosine-containing proteins in Dictyostelium discoideum were detected by immunoblot analysis and immunoprecipitation using a monoclonal anti-phosphotyrosine antibody. The iodinated antibody recognized on bots a cluster of 205-220 kDa polypeptides and bands of 107 and 60 kDa. The 107 and 60 kDa polypeptides and, in addition, a 82 kDa one became phosphorylated on tyrosine when the immunoprecipitate was incubated with [gamma-32P]ATP. In preparations from differentiating cells the intensity of the label was increased in the 60 kDa band and decreased in the 107 and 205-220 kDa bands.
Max-Planck-Institut für Biochemie, Martinsried bei München, FRG.
A
A
Schweiger
O
O
Mihalache
A
A
Muhr
I
I
Adrian
D000911:Antibodies, Monoclonal; D004023:Dictyostelium / Q000032:analysis*; D005656:Fungal Proteins / Q000032:analysis*; D008970:Molecular Weight; D010750:Phosphoproteins / Q000032:analysis*; D019000:Phosphotyrosine; D011233:Precipitin Tests; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D005656:Fungal Proteins; D010750:Phosphoproteins; D019000:Phosphotyrosine; D014443:Tyrosine