Individual NK cell clones lyse both tumor cell targets and herpes simplex virus-infected fibroblasts in the absence of interferon.
The target specificity of natural killer (NK) cells for either tumor cells or virus-infected cells has been investigated. Lymphocyte clones with the surface phenotype of NK cells (CD3-, CD16+) were obtained by limiting dilution of peripheral blood mononuclear cells stimulated with PHA, Herpes simplex virus type 1 (HSV-1), or Varicella-Zoster antigens. Clones were maintained in media with recombinant interleukin 2 (IL-2). Both NK-sensitive (K562 cells) and NK-resistant (Raji cells) targets were lysed by three cloned lines of NK cells. The ability to lyse NK-resistant target cells was largely lost when the cloned lymphocytes were cultured overnight in the absence of IL-2. Effector cells from all three clones were also capable of specifically lysing HSV-1 infected human fibroblasts in comparison with uninfected fibroblasts. We also showed that lysis of HSV-1 infected targets by NK cloned cells was independent of interferons in the culture system.
Canessa
A
A
Department of Pediatrics, University of Alabama.
Chatterjee
S
S
Whitley
R J
RJ
Prasthofer
E F
EF
Grossi
C E
CE
Tilden
A B
AB
D000328:Adult; D000954:Antigens, Surface / Q000276:immunology; D002461:Cell Line, Transformed / Q000276:immunology; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005347:Fibroblasts; D006561:Herpes Simplex / Q000097:blood; D006562:Herpes Zoster / Q000097:blood; D006801:Humans; D007372:Interferons / Q000378:metabolism* / Q000502:physiology*; D007376:Interleukin-2 / Q000502:physiology; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D010641:Phenotype; D010835:Phytohemagglutinins / Q000494:pharmacology; D011994:Recombinant Proteins; D018139:Simplexvirus / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011994:Recombinant Proteins; D007372:Interferons