Morphology of presumptive rapidly adapting receptors in the rat bronchus.
The present investigation was undertaken in rats to determine whether sensory nerves exist in apposition to the bronchial microvessels which may function as rapidly adapting receptors (RAR). The primary and secondary bronchi on both sides were removed and processed for light and electron microscopy. Nerves were frequently found in relation to venules external to the muscle coat of bronchi. They comprised myelinated axons which ended individually as non-myelinated convoluted terminals enclosed within a loose capsule of attenuated cells. Serial sections showed that these terminals were not related to ganglion cells. Cervical vagal section and injection of HRP-WGA into the nodose ganglion provided corroborative evidence of the sensory nature of these terminals. Vagal section caused degenerative changes in the encapsulated nerve terminals in the bronchial walls and horseradish peroxidase labelling was demonstrable in such terminals. Moreover, immunocytochemical studies demonstrated the presence of calcitonin gene regulated peptide and substance P in these structures. It is suggested that they comprise the RAR. Encapsulated nerve terminals were not found in the epithelial layer, in the submucous coat or in the muscularis of bronchi.
Department of Anatomy, University of Cambridge.
C T
CT
Kappagoda
J N
JN
Skepper
L
L
McNaughton
E E
EE
Siew
V
V
Navaratnam
D000818:Animals; D001370:Axonal Transport; D001980:Bronchi / Q000098:blood supply / Q000294:innervation*; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000648:ultrastructure; D009620:Nodose Ganglion / Q000648:ultrastructure; D011667:Pulmonary Veins / Q000033:anatomy & histology; D051381:Rats; D011919:Rats, Inbred Strains; D011984:Sensory Receptor Cells / Q000032:analysis / Q000648:ultrastructure*; D013373:Substance P / Q000032:analysis; D014628:Vagotomy; D014699:Venules / Q000033:anatomy & histology / Q000294:innervation
D013373:Substance P; D015740:Calcitonin Gene-Related Peptide