Effects of various metals on DNA synthesis and lymphokines production by human peripheral blood lymphocytes in vitro.
1. The presence of Cd2+, Ni2+, Cu2+, Mn2+ and Co2+ (10(-4)-10(-6) M) caused a dose-dependent inhibition of DNA synthesis in phytohemagglutinin (PHA) stimulated peripheral blood mononuclear cells (PBMC) from normal human donors. No inhibition of DNA synthesis was observed in the presence of Mg2+ and Al3+. 2. Spontaneous and PHA induced interleukin-1 (IL-1) was enhanced in the presence of Mn2+. 3. The release of interleukin-2 (IL-2) and soluble interleukin-2 receptors (sIL-2R) was decreased by Cd2+, Cu2+ and Mn2+. 4. Spontaneous tumor necrosis factor-alpha (TNF-alpha) production was enhanced by Co2+ and Al3+, but decreased by Cd2+. 5. gamma-Interferon (gamma-IFN) production was decreased in the presence of Cd2+ and Cu2+ and increased in the presence of Al3+.
First Department of Internal Medicine, School of Medicine, University of Athens, Laikon Hospital, Greece.
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S
Theocharis
A
A
Margeli
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P
Panayiotidis
D000535:Aluminum / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology; D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008222:Lymphokines / Q000096:biosynthesis*; D008345:Manganese / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D009532:Nickel / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines; D008670:Metals; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D002104:Cadmium; D003035:Cobalt; D008345:Manganese; D003300:Copper; D009532:Nickel; D007371:Interferon-gamma; D004247:DNA; D000535:Aluminum