HeLa-LAV, an epithelial cell line stably infected with HIV-1.
An HeLa-LAV cell line was established by infecting and subcloning previously described CD4-expressing HeLa cells with HIV-1. Cells of this line stably synthesize all major HIV proteins, release infectious particles of HIV-1, but do not die even after long term culture. More than 90% of the cells express the envelope protein gp120 on the surface. The cells can be easily and efficiently labeled with 51chromium, and exhibit a low spontaneous release. Because they are susceptible to killing by allogeneic cytotoxic T cells (CTL) when targeted to gp120, they ought to be a useful source of target cells in any kind of HIV-specific killing assays. The cells may also help studies on HIV replication in non-lymphatic/non-monocytic cells. The HeLa-LAV cell line will be freely available from the AIDS Research and Reference Reagent Program.
Berg
J
J
Department of Microbiology and Immunology, University of California, San Francisco 94143-0414.
Doe
B
B
Steimer
K S
KS
Wabl
M
M
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D015683:Gene Products, gag / Q000032:analysis; D015699:HIV Envelope Protein gp120 / Q000032:analysis; D015497:HIV-1; D006367:HeLa Cells / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D066298:In Vitro Techniques; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D054301:gag Gene Products, Human Immunodeficiency Virus
D015683:Gene Products, gag; D015699:HIV Envelope Protein gp120; C059827:HIV gag protein p25; D054301:gag Gene Products, Human Immunodeficiency Virus