1.3E2, a variant of the B lymphoma 70Z/3, defective in activation of NF-kappa B and OTF-2.
The mouse B-cell cell lymphoma 70Z/3 is a convenient model system in which to study the regulation of immunoglobulin synthesis. Three transcriptional activators of kappa (kappa) light chain synthesis have been identified for these cells: bacterial lipopolysaccharide (LPS), interferon-gamma (IFN), and interleukin-1 (IL-1). The response of the kappa gene in 70Z/3 cells to LPS is mediated by increases in two transcription factors: NF-kappa B and OTF-2. In contrast, IFN has no effect on either of these factors in 70Z/3 cells. We have isolated by immunoselection an LPS- IFN+ variant of 70Z/3 called 1.3E2. We show here that LPS treatment of these cells causes no increase in nuclear localization of either NF-kappa B or OTF-2. Although they have normal levels of cytoplasmic NF-kappa B, it cannot be activated by LPS or by phorbol 12-myristate 13-acetate (PMA) treatment of the cells. These experiments expand the genetic dissection of the molecular pathways of activation of kappa transcription in 70Z/3 cells.
Department of Genetics (SK-50), University of Washington, Seattle 98195.
J W
JW
Rooney
D W
DW
Emery
C H
CH
Sibley
D000818:Animals; D001402:B-Lymphocytes; D004268:DNA-Binding Proteins; D005803:Genes, Immunoglobulin; D007372:Interferons / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008223:Lymphoma / Q000235:genetics* / Q000276:immunology; D051379:Mice; D016328:NF-kappa B; D050813:Octamer Transcription Factor-2; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000032:analysis / Q000502:physiology*
D004268:DNA-Binding Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; D050813:Octamer Transcription Factor-2; C489206:Pou2f2 protein, mouse; D014157:Transcription Factors; D007372:Interferons; D013755:Tetradecanoylphorbol Acetate