Susceptibility of four species of mosquitoes to Chandipura virus and its detection by immunofluorescence.
Susceptibility of Culex tritaeniorhynchus, Cx. Bitaeniorhynchus, Cx. quinquefasciatus, and Aedes aegypti to Chandipura (CHP) virus was compared after parental inoculation of the mosquitoes. Virus detection was done by indirect immunofluorescence (IF). CHP antigen in head squashes of all the four species was seen at 24 hr post infection (p.i.). The mosquitoes supported the virus growth and transmission by bite to 2 days old suckling Swiss albino mice. Ae. aegypti which was found the most susceptible mosquito species for CHP virus can be used as a substitute for laboratory mice.
National Institute of Virology, Pune, India.
M A
MA
Ilkal
M K
MK
Goverdhan
P S
PS
Shetty
C D
CD
Tupe
M S
MS
Mavale
V
V
Dhanda
D000330:Aedes / Q000382:microbiology*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D003465:Culex / Q000382:microbiology*; D004198:Disease Susceptibility; D005455:Fluorescent Antibody Technique; D007303:Insect Vectors / Q000382:microbiology*; D051379:Mice; D012209:Rhabdoviridae / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D013045:Species Specificity; D014777:Virus Diseases / Q000382:microbiology / Q000635:transmission; D014779:Virus Replication
D000956:Antigens, Viral