[Development of highly sensitive enzyme immunoassay to measure urinary beta-core fragment in patients with gynecological cancer].
In addition to human chorionic gonadotropin (hCG) and its free subunits, low molecular weight hCG beta-related fragments have been previously demonstrated in pregnancy urine and in the urine of patients with trophoblastic and nontrophoblastic tumors. The urinary beta-core fragment in particular is focused on as a new tumor marker in gynecological malignancies. We developed an EIA for the beta-core fragment using the monoclonal antibody (MoAb229) which specifically recognized the core portion of hCG beta. By measuring with MoAb229-EIA, it was clearly revealed that urine obtained from normal pregnant women as well as from patients with choriocarcinoma and nontrophoblastic ovarian cancer contains a large amount of the beta-core fragment when separated on Sephadex G-100. We conclude that our MoAb229-EIA is a useful tool to use in detecting the beta-core fragment, a new tumor marker, in the urine of gynecological cancer patients.
Department of Obstetrics and Gynecology, Hyogo Medical Center for Adults.
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Nishimura
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Kobayashi
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Katsuno
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T
Kobayashi
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000652:urine*; D002822:Choriocarcinoma / Q000175:diagnosis; D006063:Chorionic Gonadotropin / Q000276:immunology / Q000652:urine*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D002850:Chromatography, Gel; D005260:Female; D005833:Genital Neoplasms, Female / Q000175:diagnosis* / Q000652:urine; D006801:Humans; D007124:Immunoenzyme Techniques; D010051:Ovarian Neoplasms / Q000175:diagnosis; D010446:Peptide Fragments / Q000276:immunology / Q000652:urine*; D011237:Predictive Value of Tests; D011247:Pregnancy; D015203:Reproducibility of Results; D014594:Uterine Neoplasms / Q000175:diagnosis
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D010446:Peptide Fragments