Changes in urinary proximal tubule parameters in neonatal rats exposed to cadmium chloride during pregnancy.
Pregnant Sprague-Dawley rats were injected intraperitoneally with physiological saline solution (vehicle) or cadmium chloride (CdCl2) at 2.0 or 2.5 mg kg-1 on days 8, 10, 12 and 14 of gestation. On postnatal day (PND) 3, 12 or 49, the offspring were examined for 8- or 24-h urinary excretion of beta 2-microglobulin (beta 2-m), metallothionein (MT) and urinary activity of three proximal tubular enzymes: gammaglutamyl transferase (GGT), alkaline phosphatase (ALP) and N-acetyl-beta-glucosaminidase (NAG). Treatment with CdCl2 did not affect growth or survival of offspring. Significant decreases in the urinary excretion of GGT, ALP and NAG were observed on PND 3, at both doses. Exposure to 4 x 2.5 mg kg-1 resulted in functional deficit of the proximal tubule on PND 3, as evidenced by the significant increase in beta 2-m. Except for a slight but significant increase of beta 2-m in 49-day-old males, all the other urinary parameters returned to control values on PND 12. There was no effect on MT. Results from this study show that prenatal exposure to CdCl2 can induce significant changes in the kidney biochemistry of rats in the early postnatal period.
Institut National de Recherche et de Sécurité, Vandoeuvre, France.
A M
AM
Saillenfait
J P
JP
Payan
M T
MT
Brondeau
D
D
Zissu
J
J
de Ceaurriz
D000469:Alkaline Phosphatase / Q000652:urine; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D019256:Cadmium Chloride; D005260:Female; D005333:Fetus / Q000187:drug effects*; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D005723:gamma-Glutamyltransferase / Q000652:urine
D002104:Cadmium; D008668:Metallothionein; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D019256:Cadmium Chloride