Transformed tomato plants express a satellite RNA of cucumber mosaic virus and produce lethal necrosis upon infection with viral RNA.
Tomato plants transformed with a single copy of a tomato necrosis causing satellite RNA of cucumber mosaic virus (CMV) express the satellite sequence, but the plants show no disease symptoms and have a normal appearance. Upon challenge infection of the F1 progeny with a CMV strain free of any detectable encapsidated satellite the plants accumulated single and double-stranded forms of satellite RNA and developed lethal necrosis.
Microbiology and Plant Pathology Laboratory, P.S.I., A.R.S., U.S. Department of Agriculture, Beltsville, Maryland 20705.
P B
PB
McGarvey
J M
JM
Kaper
M J
MJ
Avila-Rincon
L
L
Peña
J R
JR
Diaz-Ruiz
D015152:Blotting, Northern; D002472:Cell Transformation, Viral; D002678:Chimera; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D009029:Mosaic Viruses / Q000235:genetics*; D009336:Necrosis; D010935:Plant Diseases; D010942:Plant Viruses / Q000235:genetics*; D010944:Plants / Q000382:microbiology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis*; D019244:RNA, Satellite; D012367:RNA, Viral / Q000096:biosynthesis*
D004279:DNA, Viral; D019244:RNA, Satellite; D012367:RNA, Viral; D012313:RNA