Use of synthetic oligonucleotide DNA probes for the identification of Bacteroides gingivalis.
Six different oligonucleotide probes complementary to the hypervariable regions of 16S rRNA of Bacteroides gingivalis were tested for specificity and sensitivity against 77 field strains of B. gingivalis and 105 strains of 12 other Bacteroides species. The data demonstrated that these probes were very specific (range, 0.85 to 1.00) and sensitive (1.00). Some limited cross-reactions with other Bacteroides species were observed. Four of these probes should be useful for rapid detection and identification of B. gingivalis.
Department of Oral Biology, University of Washington, Seattle 98195.
B J
BJ
Moncla
P
P
Braham
K
K
Dix
S
S
Watanabe
D
D
Schwartz
D001439:Bacteroides / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015342:DNA Probes; D006801:Humans; D009693:Nucleic Acid Hybridization; D010510:Periodontal Diseases / Q000382:microbiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D015342:DNA Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S