The gap junction-like form of a vacuolar proton channel component appears not to be an artifact of isolation: an immunocytochemical localization study.
Gap junctional structures containing a 16-kDa intrinsic membrane protein have been isolated from the hepatopancreas of the crustacean Nephrops norvegicus. These structures are double membranes 14-15 nm thick and composed of hexagonal arrays of particles which have a central pore that is penetrated by a cationic negative stain. Membrane preparations have also been isolated from the hepatopancreas and these contain similar gap junctional regions of uniform width. Affinity purified antibodies to the 16-kDa protein bind principally to these gap junctional regions. Antiserum raised against the isolated gap junctional structures binds strongly to the lateral surfaces of the columnar epithelial cells and in particular to gap junction-like regions.
Gatty Marine Station, Department of Biology and Preclinical Medicine, University of St. Andrews, Fife, Scotland.
B
B
Leitch
M E
ME
Finbow
D000818:Animals; D003445:Crustacea / Q000033:anatomy & histology / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D007150:Immunohistochemistry / Q000379:methods; D007365:Intercellular Junctions / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D007473:Ion Channels / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods; D014617:Vacuoles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
D007106:Immune Sera; D007473:Ion Channels; D008565:Membrane Proteins