Polyamines and polyamino acids regulation of cytosolic tyrosine protein (Tyr-P) kinase from human erythrocytes.
In vitro regulation of cytosolic tyrosine protein (Tyr-P) kinase from human erythrocytes by polyamines, polyamino acids, negative charged compounds or by insulin using angiotensin II or poly (Glu-Tyr)4:1 as substrates was studied. All the three polyamines, putrescine (Put), spermidine (Spd) and spermine (Spm) stimulated the Tyr-P kinase activity in a dose dependent manner. Spm stimulated Tyr-P kinase activity higher than Put and Spd whether the substrate was angiotension II or poly (Glu-Tyr)4:1. Polyamino acids (polyornithine, polyarginine, polyglutamic acid and polyaspartic acid) did not affect significantly the Tyr-P kinase phosphorylation except polylysine which significantly stimulated the Tyr-P kinase activity. Negative charged compounds (chondroitin sulfate A, B and C) and heparin inhibited the Tyr-P kinase phosphorylation while insulin did not influence the enzyme activity in the presence of either substrates.
Unité Fonctionnelle de Biologie Cellulaire CNRS SDI, Centre Hospitalier Universitaire, Rennes, France.
N A
NA
Khan
I
I
Masson
V
V
Quemener
G
G
Clari
V
V
Moret
J P
JP
Moulinoux
D000804:Angiotensin II / Q000378:metabolism; D002807:Chondroitin / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D010766:Phosphorylation; D011073:Polyamines / Q000494:pharmacology*; D011107:Polylysine / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000097:blood*; D011700:Putrescine / Q000494:pharmacology; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D003911:Dextrans; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011073:Polyamines; D000804:Angiotensin II; D011107:Polylysine; D013096:Spermine; C027606:L-glutamic acid-L-tyrosine copolymer; D006493:Heparin; D002807:Chondroitin; D016264:Dextran Sulfate; D011505:Protein-Tyrosine Kinases; D013095:Spermidine; D011700:Putrescine