Rat and porcine galanin are equipotent in inhibiting insulin responses to glucose in the anesthetized rat.
The structure of rat galanin, recently elucidated using recombinant DNA technology, differs from porcine galanin by three amino acid residue substitutions at positions located in the C-terminal region. A synthetic replicate of the proposed structure of rat galanin was prepared and its potency to inhibit insulin responses to glucose in anesthetized rats was compared with that of porcine galanin. Within experimental error, the dose-response curves of porcine and rat galanin to inhibit glucose-stimulated rat insulin responses were indistinguishable. The ED50 dose of porcine galanin was 0.6 micrograms/100 g body weight and for rat galanin 0.8 micrograms/100 g body weight. These results suggest that the C-terminal region of the molecule is not essential for galanin's potent inhibitory action on insulin responses to glucose administration.
Department of Physiology, University of Western Ontario, London, Canada.
E M
EM
Schnuerer
A
A
Rökaeus
M
M
Carlquist
T
T
Bergman
J
J
Dupré
T J
TJ
McDonald
D000595:Amino Acid Sequence; D000818:Animals; D004305:Dose-Response Relationship, Drug; D019004:Galanin; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship; D013552:Swine
D007328:Insulin; D010455:Peptides; D019004:Galanin; D005947:Glucose