A potato mitochondrial isoleucine tRNA is coded for by a mitochondrial gene possessing a methionine anticodon.
The nucleotide sequence of a potato mitochondrial tRNA(Ile) has been determined. This tRNA has a lysidine-like minor nucleotide in the first position of the anticodon (position 34). The corresponding mitochondrial gene has been cloned and sequenced. This gene has a CAT anticodon which corresponds to methionine. The C residue in the anticodon must therefore be post-transcriptionally modified. The mature tRNA has isoleucine-accepting activity but no methionine-accepting activity. This is the first report showing that in plant mitochondria a post-transcriptional modification could change the amino-acid specificity of a tRNA, as compared to that of the corresponding gene.
Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
F
F
Weber
A
A
Dietrich
J H
JH
Weil
L
L
Maréchal-Drouard
D000926:Anticodon; D001483:Base Sequence; D015139:Blotting, Southern; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D000077278:RNA, Mitochondrial; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012355:RNA, Transfer, Ile / Q000235:genetics* / Q000378:metabolism; D012358:RNA, Transfer, Met / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D011198:Solanum tuberosum / Q000235:genetics*
D000926:Anticodon; D004272:DNA, Mitochondrial; D000077278:RNA, Mitochondrial; D012345:RNA, Transfer, Amino Acid-Specific; D012355:RNA, Transfer, Ile; D012358:RNA, Transfer, Met; D012313:RNA