A micromethod for the isolation of total RNA from adipose tissue.
We have developed a simple and rapid procedure for the isolation of total RNA from small amounts of adipose tissue. Using this method, it is possible to obtain quantitative recovery of RNA from less than 300 mg of adipose tissue, with an average yield of 70 micrograms of RNA per gram of adipose tissue. Northern blot analysis of rat epididymal adipose tissue RNA samples was performed using a beta-actin probe and demonstrated that intact total RNA had been isolated. The procedure has been adapted for use in 1.5-ml microcentrifuge Eppendorf tubes, providing a convenient and inexpensive method for the reproducible recovery of intact RNA from sparse samples of adipose tissue.
Division of Endocrinology, Stanford University School of Medicine, California 94305.
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Tavangar
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Hoffman
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Kraemer
D000273:Adipose Tissue / Q000032:analysis*; D000818:Animals; D015152:Blotting, Northern; D002498:Centrifugation; D006146:Guanidines; D008297:Male; D012313:RNA / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D013861:Thiocyanates
D006146:Guanidines; D013861:Thiocyanates; C054436:guanidine thiocyanate; D012313:RNA