[A comparison of pyrophosphatase and peroxidase as markers in the immunoenzyme analysis of the tick-borne encephalitis virus and antibodies to it].
The authors compare the sensitivities of pyrophosphatase and peroxidase conjugates with immunoglobulins in assays of tick-borne encephalitis (TBE) virus antigens and anti-TBE antibodies in human sera. Pyrophosphatase conjugates are similar to peroxidase ones in their enzymic and immunochemical characteristics and are not inferior to them in sensitivity in titration of antibodies and superior in assays with antigens. A number of advantages of pyrophosphatase conjugates, i.e. a simple preparation procedure, stable substrate and enzymic reaction product, color range convenient for visual recording, etc., make these conjugates preferable vs. the peroxidase ones and recommend them for wide use at diagnostic laboratories.
V N
VN
Shchipakin
O A
OA
Evtushenko
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D010544:Peroxidases; D011755:Pyrophosphatases
D000914:Antibodies, Viral; D000956:Antigens, Viral; D010544:Peroxidases; D011755:Pyrophosphatases
Sravnenie pirofosfatazy i peroksidazy kak markerov v immunofermentnom analize virusa kleshchevogo éntsefalita i antitel k nemu.