In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs.
Mutating the CAACCCCAA sequence in the RNA component of telomerase causes the synthesis in vivo of new telomere sequences corresponding to the mutated RNA sequence, demonstrating that the telomerase contains the template for telomere synthesis. These mutations also lead to nuclear and cell division defects, and senescence, establishing an essential role for telomerase in vivo.
Yu
G L
GL
Department of Molecular and Cell Biology, University of California, Berkeley.
Bradley
J D
JD
Attardi
L D
LD
Blackburn
E H
EH
D000818:Animals; D001483:Base Sequence; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D015870:Gene Expression; D005796:Genes; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D012313:RNA / Q000235:genetics*; D013698:Templates, Genetic; D013768:Tetrahymena / Q000201:enzymology / Q000235:genetics*
D012313:RNA; D004253:DNA Nucleotidylexotransferase