Breast biopsy nuclear pellets are a convenient source of DNA for routine determination of Her-2/neu gene amplification.
Cell nuclei from breast biopsies are a byproduct of routine hormone receptor determination and are usually discarded. We report here that DNA purified from this source is suitable for use in conventional Southern blot or Polymerase Chain Reaction (PCR) techniques to screen for Her-2/neu amplification, enabling efficient use of limited biopsy material.
Department of Oncology, Soroka Medical Center, Beer-Sheva, Israel.
O
O
Malka
Y
Y
Pollack
D
D
Benharroch
Y
Y
Cohen
R
R
Levy
I
I
Yanai-Inbar
J
J
Gopas
D001706:Biopsy; D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002467:Cell Nucleus / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005784:Gene Amplification / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D008970:Molecular Weight; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D016133:Polymerase Chain Reaction; D018719:Erb-b2 Receptor Tyrosine Kinases
D004273:DNA, Neoplasm; D009856:Oncogene Proteins, Viral; D018719:Erb-b2 Receptor Tyrosine Kinases