Genomic heterogeneities in bovine herpesvirus type 1 viral isolates: a major variant selected from a field isolate.
We have analyzed viral DNA patterns, obtained following digestion of six different infectious bovine rhinotracheitis isolates, using a panel of restriction enzymes. Several differences in profiles were observed, particularly for a Canadian viral strain which presumably contained a genome that was larger than the other five infectious bovine rhinotracheitis genomes. Also, an insertion/deletion of a HindIII restriction site was found in the DNA of a Danish strain; this additional HindIII site was localized precisely within the original HindIII E fragment of the infectious bovine rhinotracheitis virus. Interestingly, results could be used to distinguish each viral strain examined from the others by simple digestion with the appropriate enzyme.
Centre de Recherche en Virologie, Université du Québec, Ville de Laval, Canada.
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C
Simard
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LaBoissière
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Séguin
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M
Trudel
D004279:DNA, Viral / Q000378:metabolism; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D005814:Genes, Viral; D014644:Genetic Variation; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII