Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin.
Nineteen monoclonal antibodies which bind to native dystrophin in the plasma membrane of frozen muscle sections were obtained using a recombinant fusion protein as immunogen. On Western blots of normal mouse muscle extracts, the antibodies bind specifically to a 400,000 Mr protein which is absent from dystrophic mouse (mdx) muscle. At least four distinct epitopes have been identified by cleavage mapping methods. Although the fusion protein contained 25% of the human dystrophin sequence (Cys816-Asp1747; Mr 108,000), most of the monoclonal antibodies (15 out of 19) recognize a single fragment of Mr 27,500.
Research Division, N.E. Wales Institute, Deeside, Clwyd, U.K.
Nguyen thi Man
A J
AJ
Cartwright
G E
GE
Morris
D R
DR
Love
J F
JF
Bloomfield
K E
KE
Davies
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D016189:Dystrophin; D000939:Epitopes; D006801:Humans; D051379:Mice; D009124:Muscle Proteins / Q000276:immunology; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology
D000911:Antibodies, Monoclonal; D016189:Dystrophin; D000939:Epitopes; D009124:Muscle Proteins; D011993:Recombinant Fusion Proteins