A novel peptide isolated from the leaves of Gymnema sylvestre--I. Characterization and its suppressive effect on the neural responses to sweet taste stimuli in the rat.
1. A new substance that suppressed selectively the neural responses of the rat to sweet taste stimuli was isolated from the leaves of Gymnema sylvestre. 2. The substance was proved to be a peptide consisting of 35 amino acids and having a molecular weight of about 4,000. 3. The inhibitory effect on the sweet responses appeared after treating the tongue surface with the peptide at a concentration of more than 1 x 10(-6) M.
Department of Physiology, Tottori University School of Medicine, Yonago, Japan.
T
T
Imoto
A
A
Miyasaka
R
R
Ishima
K
K
Akasaka
D000818:Animals; D002814:Chorda Tympani Nerve / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007564:Japan; D009682:Magnetic Resonance Spectroscopy; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000737:chemistry*; D051381:Rats; D011919:Rats, Inbred Strains; D013649:Taste / Q000187:drug effects*
D010940:Plant Proteins