[Polyclonal activated circulating B-lymphocytes in patients with rheumatoid arthritis].
Peripheral B lymphocytes were studied to investigate the role of polyclonal B-cell activation in the pathogenesis of rheumatoid arthritis (RA). Proliferative responses to anti-mu antibodies coupled to sepharose used as a B-cell-specific mitogen were reduced as compared to healthy controls. When cell surface antigens were studied by flow cytometry employing monoclonal antibodies, B cells from patients with RA showed enhanced expression of Interleukin-2 (IL-2) receptor. In-vivo-activated, in-vitro spontaneously proliferating Epstein-Barr virus transformed B cells positive for immunoglobulin M, G, and A were obtained from patients with RA. In summary, our findings suggest the presence of polyclonal activated B cells in the peripheral blood of patients with RA, and a close relationship of polyclonal activated B cells and the activity of the disease process.
Med. Klinik III u. Poliklinik, Universität Giessen.
H
H
Becker
C
C
Weber
M
M
Renelt
K
K
Federlin
D000328:Adult; D000368:Aged; D000943:Antigens, Differentiation / Q000032:analysis; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D018956:CD5 Antigens; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D015375:Receptors, Interleukin-2 / Q000032:analysis
D000943:Antigens, Differentiation; D018956:CD5 Antigens; D007075:Immunoglobulin M; D015375:Receptors, Interleukin-2
Polyklonal aktivierte zirkulierende B-Lymphozyten bei Patienten mit rheumatoider Arthritis.