Bluetongue virus: surface exposure of VP7.
The exposed proteins of bluetongue virus serotype 17 were determined using surface labeling and reactivity with monoclonal antibodies. Iodination of amino groups predominantly labeled VP2; however, iodination of tyrosine residues labeled both VP2 and VP5, with VP7 labeled to a significantly lesser degree. To investigate the exposure of VP7 on the intact virion further, monoclonal antibodies that reacted with this protein were used. At least two antibodies, reacting with different epitopes on VP7, bound to intact virions, as determined by adsorption of infectious particles, electron microscopic observation of antibody-bound virus, and co-sedimentation of antibody and virus. Surface iodination of viral cores was used to show that VP7 and VP3 are major exposed proteins on these particles. We conclude that a major core protein, VP7, has at least two epitopes exposed on the virus surface.
United States Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944.
S A
SA
Lewis
M J
MJ
Grubman
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D001820:Bluetongue virus / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D000939:Epitopes / Q000032:analysis; D012087:Reoviridae / Q000032:analysis*; D014758:Viral Core Proteins / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D014758:Viral Core Proteins