Structural and immunological properties of the phenolic glycolipids from Mycobacterium gastri and Mycobacterium kansasii.
Mycobacterial species-specific antigens belong to the three following classes: phenolic glycolipids (Phe Gl), acyltrehalose-containing lipooligosaccharides and polar glycopeptidolipids. These antigens have been chemically defined and alkali-labile epitopes were found to characterize the lipooligosaccharide antigen type. In the present study the major Mycobacterium kansasii phenolic glycolipid epitope namely Phe Gl K-I was delineated as the distal monoacetylated disaccharidic residue: 2,6-dideoxy-4-O-methyl-alpha-D-arabino-hexopyranosyl-(1----3)-2-O-methyl -4-O- acetyl-alpha-L-fucopyranose. This acetoxy group is required for K-I epitope recognition demonstrating that alkali-labile epitopes also occur in the phenolic glycolipid antigen class. Using immunoelectron microscopy, the Phe Gl K-I epitope was localized around the electron-transparent layer on the M. kansasii cell-wall surface. Furthermore, two new phenolic glycolipids namely Phe Gl K-III and Phe Gl K-IV were discovered in minute amounts. They were purified and characterized by their retention time in direct-phase column HPLC. These molecules are also M. kansasii antigens, whose epitopes differ from that of Phe Gl K-I. The complete family of phenolic glycolipids Phe Gl K-I, K-II, K-III and K-IV was found in both rough and smooth variants of both M. kansasii and Mycobacterium gastri species.
Gilleron
M
M
Centre de Recherche de Biochimie et de Génétique Cellulaires du Centre National de la Recherche Scientifique, Toulouse, France.
Venisse
A
A
Fournie
J J
JJ
Riviere
M
M
Dupont
M A
MA
Gas
N
N
Puzo
G
G
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D006017:Glycolipids / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D008854:Microscopy, Electron; D009161:Mycobacterium / Q000276:immunology* / Q000648:ultrastructure; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification; D013045:Species Specificity
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D000939:Epitopes; D006017:Glycolipids; D010636:Phenols