Seminalplasmin, the major basic protein of bull seminal plasma, is a secretory protein of the seminal vesicles.
From the experimental results of three independent methods: (1) indirect immunofluorescence employing monospecific anti-seminalplasmin-IgGs, (2) cell-free translation of poly(A)+ RNA from seminal vesicle and testicular tissue, as well as (3) Northern analysis of poly(A)+ RNA of the latter tissues with a synthetic seminalplasmin-specific antisense DNA probe, it is concluded that the biosynthesis of seminalplasmin occurs in seminal vesicles but not in testis.
Wempe
F
F
Max-Planck-Institute für biophysikalische Chemie, Abteilung Molekulare Biologie, Göttingen, F.R.G.
Wagner
S
S
vd Kammer
H
H
Krauhs
E
E
Scheit
K H
KH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002474:Cell-Free System; D015342:DNA Probes; D005455:Fluorescent Antibody Technique; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012661:Semen / Q000032:analysis*; D029607:Seminal Vesicle Secretory Proteins; D012669:Seminal Vesicles / Q000378:metabolism*; D013737:Testis / Q000378:metabolism
D015342:DNA Probes; D011506:Proteins; D012333:RNA, Messenger; D029607:Seminal Vesicle Secretory Proteins; D011061:Poly A; D012313:RNA