Inactivation of HIV-1 by trypsin and its use in demonstrating specific virus infection of cells.
HIV-1 was found sensitive to inactivation by low concentrations of trypsin. The use of trypsin was valuable for assessing non-specific binding of HIV virions to CD4+ cells. This effect was also helpful for eliminating input virus in experiments studying HIV infection of cells in vitro.
Cancer Research Institute, University of California, School of Medicine, San Francisco 94143-0128.
S B
SB
Tang
J A
JA
Levy
D000998:Antiviral Agents / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000382:microbiology; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology; D015658:HIV Infections / Q000382:microbiology; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D014357:Trypsin / Q000494:pharmacology*; D014773:Virology / Q000379:methods
D000998:Antiviral Agents; D014357:Trypsin