Immunolocalization of basement membrane molecules in the stroma of salivary gland pleomorphic adenoma.
In order to determine the participation of basement membrane molecules in formation of its characteristic stroma, 30 cases of salivary gland pleomorphic adenoma were examined by immunohistochemical staining for type IV collagen, laminin, heparan sulfate proteoglycan, and entactin. The stroma was histopathologically classified into four types: hyaline, fibrous, myxoid, and chondroid. Immunohistochemically, type IV collagen and laminin were more intensively localized in hyaline, fibrous and chondroid types of stroma, whereas heparan sulfate proteoglycan was more prominent in myxoid areas. The results suggest that the stroma contains these basement membrane components, which are possibly biosynthesized by epithelial tumor cells, and that histological variety of the stroma depends on proportion of local contents of each basement membrane molecule.
Department of Pathology, Nagasaki University School of Dentistry, Japan.
T
T
Saku
J
J
Cheng
H
H
Okabe
Z
Z
Koyama
D008949:Adenoma, Pleomorphic / Q000032:analysis* / Q000145:classification / Q000648:ultrastructure; D000906:Antibodies; D001485:Basement Membrane / Q000032:analysis*; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis; D003094:Collagen / Q000032:analysis; D004848:Epithelium / Q000032:analysis / Q000648:ultrastructure; D006023:Glycoproteins / Q000032:analysis; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000032:analysis; D006801:Humans; D006818:Hyalin / Q000032:analysis; D007124:Immunoenzyme Techniques; D007797:Laminin / Q000032:analysis; D008562:Membrane Glycoproteins; D012468:Salivary Gland Neoplasms / Q000032:analysis* / Q000145:classification / Q000648:ultrastructure; D013194:Staining and Labeling
D000906:Antibodies; D011508:Chondroitin Sulfate Proteoglycans; D003094:Collagen; D006023:Glycoproteins; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D007797:Laminin; D008562:Membrane Glycoproteins; C029967:nidogen