[Identification of HLA class II polymorphism with molecular biology techniques].
All the biologically relevant HLA class II allelic variants can not be identified with conventional serological tissue typing techniques. During the past few years considerable advances have been made in HLA class II typing with molecular techniques now widely used in routine clinical tissue typing. The next few years are likely to see the development of tissue typing techniques based on the polymerase chain reaction (PCR), for use in acute transplantation. A new method for the rapid identification of genetic polymorphisms is described--allele-specific PCR amplification.
Samtliga arbetar vid institutionen för klinisk immunologi, Karolinska institutet, Huddinge sjukhus.
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Olerup
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Zetterquist
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Möller
D006681:HLA-D Antigens / Q000235:genetics* / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008967:Molecular Biology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
D006681:HLA-D Antigens
Identifiering av HLA klass II-polymorfism med molekylärbiologiska metoder.