Affinity enhancement of bispecific antibody against two different epitopes in the same antigen.
For the enhancement of antibody binding affinity, a bispecific antibody against two different epitopes in human chorionic gonadotropin hormone, one is in alpha-subunit and the other is in beta-subunit, was prepared by chemical recombination using 5,5'-dithiobis(2-nitrobenzoic acid). The epitopes recognized by antibodies were investigated by competitive radioimmunoassay, two-site sandwich radioimmunoassay and additivity assay and a proper epitope pair was chosen for preparation of the bispecific antibody. This bispecific antibody has dual specificity and as much as 17.2-fold higher affinity than that of monoclonal antibody with higher affinity by dual antigen binding radioimmunoassay and Scatchard plot analysis.
Cheong
H S
HS
Immunology Laboratory, Mogam Biotechnology Research Institute, Kyonggi-Do, Korea.
Chang
J S
JS
Park
J M
JM
Byun
S M
SM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000941:Antigens / Q000276:immunology*; D001667:Binding, Competitive; D006063:Chorionic Gonadotropin / Q000276:immunology*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D004228:Dithionitrobenzoic Acid; D000939:Epitopes; D005260:Female; D015292:Glycoprotein Hormones, alpha Subunit / Q000276:immunology*; D007118:Immunoassay; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000941:Antigens; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D000939:Epitopes; D015292:Glycoprotein Hormones, alpha Subunit; D010446:Peptide Fragments; D004228:Dithionitrobenzoic Acid