Application of a novel 1:1 gold(I)-chromophoric thiolate complex as a spectrophotometric probe for the thiol-exchange reactions of anti-arthritic gold drugs in biological fluids.
An oligomeric 1:1 gold(I) complex of the chromophoric thiol 5-mercapto (2-nitrobenzoate) has been synthesized and applied as a spectrophotometric probe for the thiol-exchange reactions of structurally-analogous 1:1 gold(I)-thiolate drugs. For low-molecular-mass thiols, results were consistent with the initial formation of a monomeric mixed-ligand bis-thiolato gold(I) complex followed by further ligand substitution by excess thiol to produce 5-mercapto (2-nitrobenzoate) and a monomeric bis-thiolato gold(I) complex. For human serum albumin, however, the spectrophotometric changes were only consistent with the binding of gold(I) to its single cysteine residue (Cys-34) with the retention of the 5-mercapto (2-nitrobenzoate) ligand on gold(I).
Inflammation Group, London Hospial Medical College.
M
M
Grootveld
A W
AW
Claxson
A
A
Furst
D R
DR
Blake
D018501:Antirheumatic Agents / Q000097:blood / Q000378:metabolism* / Q000652:urine; D006046:Gold; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008024:Ligands; D009579:Nitrobenzoates; D012709:Serum Albumin / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds
D018501:Antirheumatic Agents; D007202:Indicators and Reagents; D008024:Ligands; D009579:Nitrobenzoates; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; C011136:thionitrobenzoic acid; D006046:Gold