Isolation of monoclonal antibodies monospecific for bovine alpha-lactalbumin.
Monoclonal antibodies were generated against purified bovine alpha-lactalbumin. Three antibodies reactive only to alpha-lactalbumin were selected. Two of the antibodies appeared to be species monospecific and bound with only bovine alpha-lactalbumin. The third monoclonal recognized both bovine and human alpha-lactalbumin with a 2:1 preference for bovine alpha-lactalbumin. Two of the monoclonals (48-60, 48-68) bound to different epitopes with high affinity (Ka = 8 x 10(11), 5.3 x 10(12) M-1). The third monoclonal (48-22), which was of isotype IgA, had an epitope similar to that of 48-60 but with an apparent affinity of approximately 4 x 10(12) M-1. These antibodies should prove extremely useful adjuncts in structural studies of bovine alpha-lactalbumin.
Specialized Center for Cancer Research and Education, University of Illinois School of Medicine, Chicago 60612.
K M
KM
Kuzmanoff
J W
JW
Andresen
C W
CW
Beattie
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005260:Female; D006825:Hybridomas; D007768:Lactalbumin / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007768:Lactalbumin