Role of lymphocyte activation by substance P in rheumatoid arthritis.
Peripheral mononuclear cells taken from rheumatoid arthritis (RA) patients, which had previously been treated in vitro with the neuropeptide Substance P (SP) followed by stimulation with the mitogen PHA, showed a significantly higher percent positivity of lymphocytes with the double-markers CD4+ CD2+, CD8+ CD25+, CD4+, HLA-DR+ and CD8+ HLA-DR+ than those of the cells untreated by SP. No effect of SP was detected in normal controls, except that the cells with the double-marker CD4+ CD25+ were slightly increased. Furthermore, in RA patients the percentage of CD4+ CD45R- cells was increased, while that of CD4+ CD45R+ cells was decreased, by pre-treatment with SP and stimulation with PHA. Monocytes of patients with rheumatoid arthritis after the treatment with SP released a significantly higher amount of oxygen-intermediate than those of normal controls with or without SP. From these results, it could be considered that SP plays an important role in RA inflammation.
Department of Immunology, Kurume University School of Medicine, Fukuoka, Japan.
M M
MM
Yokoyama
K
K
Fujimoto
D015703:Antigens, CD; D000943:Antigens, Differentiation; D001172:Arthritis, Rheumatoid / Q000209:etiology / Q000276:immunology* / Q000378:metabolism; D006684:HLA-DR Antigens; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D013373:Substance P / Q000494:pharmacology* / Q000502:physiology
D015703:Antigens, CD; D000943:Antigens, Differentiation; D006684:HLA-DR Antigens; D010835:Phytohemagglutinins; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D013373:Substance P; D010100:Oxygen