Development of muscle nerve in the teleost fish, medaka.
The entire process of normal development of a muscle nerve to a muscle (middle interradial muscle) in the tail region of the medaka (Oryzias latipes) is briefly reported. The nerve was stained immunohistochemically by using anti-neurofilament protein antibodies or stained by HRP and DiI labeling methods. The muscle was stained immunohistochemically by using anti-troponin T and anti-desmin antibodies. The smallness and transparency of the medaka embryos provide us with an opportunity to examine nerve-muscle development in whole-mount specimens. Our observations suggest that prior to the appearance of the middle interradial muscle a neural pathway has established, extending from the starting point to the 'door step' of the muscle.
Ishikawa
Y
Y
Department of Anatomy, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
D000818:Animals; D002232:Carbocyanines; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry / Q000379:methods; D009046:Motor Neurons / Q000502:physiology; D024510:Muscle Development; D009132:Muscles / Q000294:innervation*; D009990:Oryzias / Q000254:growth & development*; D013127:Spinal Nerves / Q000254:growth & development; D013194:Staining and Labeling
D002232:Carbocyanines; D005456:Fluorescent Dyes; C024286:3,3'-dioctadecylindocarbocyanine; D006735:Horseradish Peroxidase