Inactivation of cGMP-dependent conductance of rod outer segment plasma membrane induced by cGMP.
Integral cGMP-dependent currents as well as activity of single cGMP-activated channels in plasma membrane of rod outer segment (ROS) were recorded using the patch-clamp method. The dependence of integral currents on cGMP concentration is shown to be bell-shaped. Decrease in cGMP-dependent conductance at high cGMP concentration results from a decrease in channel opening probability. Thus, cGMP in high concentrations inactivates cGMP-dependent conductance.
Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region.
A B
AB
Zhainazarov
S S
SS
Kolesnikov
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D006152:Cyclic GMP / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity / Q000187:drug effects; D004594:Electrophysiology; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D010786:Photoreceptor Cells / Q000378:metabolism*; D011898:Ranidae; D012374:Rod Cell Outer Segment / Q000187:drug effects / Q000378:metabolism*; D014982:Xenopus laevis
D007473:Ion Channels; D006152:Cyclic GMP