Antigenic variation of human and bovine parainfluenza virus type 3 strains.
Three human and six bovine parainfluenza virus type 3 (PIV3) strains were examined by the use of 60 monoclonal antibodies (MAbs). Fifty-three MAbs to the human C243 strain were directed against six, four, nine and seven epitopes of the haemagglutinin-neuraminidase (HN), fusion (F), nucleocapsid (N) and matrix proteins, respectively. Seven MAbs to the bovine strain were directed against three epitopes of the HN protein and three epitopes of the F protein. Each strain was characterized in ELISA and immunofluorescence tests with all MAbs and in a haemagglutination inhibition assay with the anti-HN MAbs. There were marked differences between human and bovine viruses, primarily in the HN protein where five epitopes differed. One epitope of the F and one of the N protein also differed. Bovine PIV3 was found to be a homogeneous subtype and distinct from human PIV3.
Department of Virology, Karolinska Institute, School of Medicine, Stockholm, Sweden.
E
E
Klippmark
R
R
Rydbeck
H
H
Shibuta
E
E
Norrby
D000818:Animals; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000235:genetics*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D010224:Parainfluenza Virus 3, Human / Q000276:immunology*; D018108:Respirovirus / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D014764:Viral Proteins