[Vasoactive intestinal peptide induces 2',5'-oligoadenylate synthetase and antiviral state in cells of HT-29 colonic cancer].
Vasoactive Intestinal Peptide (VIP) is able at the concentration 10 to 100 nM to induce in HT-29 cells 2'5' oligoadenylate (2'5' A) synthetase activity. The kinetics of this induction show that the maximal effect is attained after 24 hrs. VIP induces 2'5' A synthetase parallel to inhibition of vesicular stomatitis virus growth. The levels of these two induced activities after VIP treatment are comparable to those induced by the poly (I).poly (C), an inducer of IFN beta/alpha in mammalian cells. Moreover the anti-IFN beta/alpha antibodies abolish the VIP-induced 2'5' A synthetase whereas anti-IFN gamma antibodies are ineffective. The fact that VIP establishes an antiviral state in HT-29 cells potentiates new pharmaceutical applications for this neuropeptide.
I.N.S.E.R.M., Hôpital Saint-Antoine, Paris.
M K
MK
Chelbi-Alix
C
C
Boissard
G
G
Rosselin
M N
MN
Thang
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis*; D000818:Animals; D002460:Cell Line; D003110:Colonic Neoplasms / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D007372:Interferons / Q000378:metabolism; D051379:Mice; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology* / Q000502:physiology; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology*; D014779:Virus Replication / Q000187:drug effects
D014660:Vasoactive Intestinal Peptide; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase
Le peptide intestinal vasoactif induit la 2'5' oligoadénylate synthétase et l'état antiviral dans les cellules du cancer du colon HT-29.