High-performance liquid chromatographic determination of D-amino acid oxidase activity.
A new procedure for the assay of D-amino acid oxidase activity has been developed. alpha-Ketoisovaleric acid, derived from D-valine, was estimated by high-performance liquid chromatography after reaction with o-phenylenediamine to give the corresponding quinoxalinol derivative. alpha-Ketovaleric acid was used as an internal standard to ensure the reproducibility of the method. As an example of application, kidney cortex homogenates were analyzed for their D-amino acid oxidase activity. The advantages of the presented procedure for the determination of the enzymatic activity in biological samples compared with previously reported procedures are discussed.
Biondi
P A
PA
Institute of Veterinary Physiology and Biochemistry, Milan, Italy.
Guidotti
L
L
Negri
A
A
Secchi
C
C
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003605:D-Amino-Acid Oxidase / Q000737:chemistry*; D045782:Hemiterpenes; D007651:Keto Acids / Q000032:analysis / Q000737:chemistry; D007668:Kidney / Q000201:enzymology; D010421:Pentanoic Acids / Q000737:chemistry; D010655:Phenylenediamines; D013552:Swine; D014631:Valerates / Q000737:chemistry
D045782:Hemiterpenes; D007651:Keto Acids; D010421:Pentanoic Acids; D010655:Phenylenediamines; D014631:Valerates; C001505:alpha-ketoisovalerate; D003605:D-Amino-Acid Oxidase