Organization of the cytoskeleton and the focal contacts of bovine aortic endothelial cells cultured on type I and III collagen.
We investigated the organization of the cytoskeleton and the focal contacts of bovine aortic endothelial cells cultured on type I and III collagen. The influence of these collagens on cell morphology and the distribution pattern of actin, vimentin, talin, and vinculin was analyzed by light microscopy, conventional electron microscopy, immunofluorescence, and immunogold labeling after lysis-squirting. Whereas the morphology of the endothelial cells is not markedly influenced, the structure of the cytoskeleton and the focal contacts of the cells are altered by the different collagen types. Stress fibers are more distinct in cells grown on type I collagen; cells on type III collagen show a more diffuse distribution of actin molecules. Intermediate filaments seem not to be affected by the collagens. The areas of focal contacts are larger in cells on type I collagen. Additionally, the labeling pattern of talin and vinculin is denser in focal contacts of cells grown on type I collagen. These results suggest an important role of the type of collagen in mediation of the organization of the microfilament system and the adhesion structures of bovine aortic endothelial cells in culture.
Institute for Arteriosclerosis Research, Unit for Cell Biology and Ultrastructural Research, University of Muenster, Federal Republic of Germany.
R
R
Semich
H
H
Robenek
D000199:Actins / Q000378:metabolism; D000818:Animals; D001011:Aorta; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism* / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008840:Microfilament Proteins / Q000378:metabolism*; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D013194:Staining and Labeling; D016608:Talin; D014746:Vimentin / Q000378:metabolism; D016596:Vinculin
D000199:Actins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D016608:Talin; D014746:Vimentin; D016596:Vinculin; D003094:Collagen