Micronucleus test on quinacrine dihydrochloride in mice: a comparison of dosage regimens.
Micronucleus induction by quinacrine dihydrochloride (Q) was tested in CD-1 male mice by single, double, and triple oral treatment(s) with 50 or 250 mg/kg. The mice were killed 24 h after the last treatment. Femoral marrow cells were analyzed on slides stained with Giemsa and acridine orange. Both staining methods gave similar results. The frequencies of micronucleated polychromatic erythrocytes (MPE) were marginally increased, in comparison with a concurrent negative control group, only in the triple treatment group of 250 mg/kg. There were, however, no big differences in MPE frequencies among the treatment regimens.
Hitotsumachi
S
S
Drug Safety Research Laboratories, Takeda Chemical Industries, Osaka, Japan.
Kimura
Y
Y
Nakajima
Y
Y
Hayashi
M
M
Ishidate
M
M
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests / Q000379:methods; D011796:Quinacrine / Q000008:administration & dosage / Q000494:pharmacology*; D012016:Reference Values; D013194:Staining and Labeling
D011796:Quinacrine