Immunogenicity of a purified fragment of 17D yellow fever envelope protein.
Information on the immunogenic properties of purified flavivirus proteins may be useful in the development of recombinant or synthetic peptide vaccines. Using a monoclonal antibody, an attempt was made to purify the envelope (E) protein of 17D yellow fever virus (17D YF) by affinity chromatography. The purified material could not be identified as intact E protein but it did bear antigenic determinants of E as determined by selective reactivity with anti-E monoclonal antibodies. Rabbits immunized with this material produced antibodies that neutralized 17D YF and dengue-2 viruses in comparable titers, indicating that cross-reactive antigenic determinants were preserved. Immunization of mice resulted in protection against intracerebral challenge with 17D YF.
Brandriss
M W
MW
Rochester General Hospital, New York.
Schlesinger
J J
JJ
Walsh
E E
EE
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000096:biosynthesis*; D001345:Autoradiography; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D007114:Immunization; D051379:Mice; D011233:Precipitin Tests; D011817:Rabbits; D015531:Radioimmunoprecipitation Assay; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000302:isolation & purification; D014765:Viral Vaccines / Q000276:immunology*; D015004:Yellow Fever / Q000517:prevention & control*; D015005:Yellow fever virus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins; D014765:Viral Vaccines