Rapid induction of polyadenylate binding protein and stimulation of translational initiation in pituitary tumor cells exposed to phorbol ester.
1. GH3 pituitary cells treated for 1-2 hr with phorbol myristate acetate exhibited accumulation of large polysomes and increased incorporation of amino acids into all discrete protein populations. 2. Preferential incorporation into a basic 74 kDa polypeptide preceded significant augmentation of protein synthesis. Cellular content of this polypeptide correlated directly with the increase in protein synthesis. 3. Stimulations of incorporation, of polysome accumulation, and of preferential synthesis of the 74 kDa protein were eliminated by inhibitors of transcription. 4. The rapidly induced protein was identical with the ubiquitous polyadenylate-binding protein on the bases of size, isoelectric point, distribution with polysomes, and association with poly(A) + mRNA.
Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854.
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D002352:Carrier Proteins / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D003839:Deoxyadenosines / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D008715:Methionine / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects*; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000187:drug effects; D010911:Pituitary Neoplasms / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D039102:Poly(A)-Binding Proteins; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002352:Carrier Proteins; D003839:Deoxyadenosines; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D003609:Dactinomycin; D011061:Poly A; D008715:Methionine; C058120:cordycepin; D013755:Tetradecanoylphorbol Acetate