Calcium ion activation of the anion-conducting channel in the rat liver mitochondrial inner membrane.
Stimulation of the rat liver mitochondrial inner-membrane anion-conducting channel by aeration is dependent on the concentration of Ca2+ ions in the assay medium. Ca2+ activates anion conduction in both aerated and non-energised mitochondria but acts over a wider concentration range and produces a greater increase in anion-conductivity in aerated mitochondria. EGTA reverses Ca2+ stimulation but takes several seconds to act, indicating slow release of Ca2+ from the activation site possibly on the matrix side of the inner mitochondrial membrane. It is suggested that this channel may respond to hormone-induced changes in cytosolic Ca2+ concentration.
Department of Biochemistry, Faculty of Medicine, National University of Singapore.
M J
MJ
Selwyn
C L
CL
Ng
H L
HL
Choo
D000332:Aerobiosis; D000643:Ammonium Chloride; D000693:Anaerobiosis; D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000502:physiology; D007473:Ion Channels / Q000502:physiology*; D008930:Mitochondria, Liver / Q000502:physiology*; D008933:Mitochondrial Swelling / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D002712:Chlorides; D007473:Ion Channels; D000643:Ammonium Chloride; D004533:Egtazic Acid; D004492:Edetic Acid; D002118:Calcium