Release of heparin-like substance and histamine from basophilic leucocytes separated by counterflow centrifugation elutriation.
Separation of basophilic granulocytes from peripheral blood was carried out by counterflow centrifugation elutriation using a JE-6B roter. The release of histamine and heparin-like substance from basophilic leucocytes was estimated in healthy and asthmatic subjects. 1. The number of basophils was maximal (0.74 x 10(5] at a flow rate of 4.5 ml/min, and the purity reached a peak (11.8 +/- 2.0%) at a flow rate of 9 ml/min. 2. The content of histamine paralleled the number of basophils in each fraction. 3. The release mechanism of histamine induced by anti-IgE was similar to that of the heparin-like substance. 4. The release of histamine and the heparin-like substance was significantly higher in atopic asthmatics than in non-atopic cases.
Division of Medicine, Okayama University Medical School, Tottori, Japan.
Y
Y
Tanizaki
M
M
Sudo
H
H
Kitani
K
K
Kawauchi
T
T
Mifune
K
K
Takahashi
I
I
Kimura
D000293:Adolescent; D000328:Adult; D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D001249:Asthma / Q000276:immunology / Q000503:physiopathology; D001491:Basophils / Q000187:drug effects / Q000378:metabolism*; D002648:Child; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006493:Heparin / Q000378:metabolism*; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000494:pharmacology; D007958:Leukocyte Count; D008875:Middle Aged
D000888:Antibodies, Anti-Idiotypic; D007073:Immunoglobulin E; D006493:Heparin