Influence of the gamma subunit and expression system on acetylcholine receptor gating.
We have developed a partial kinetic theory for the gating of murine nicotinic acetylcholine receptors (AChRs) expressed in Xenopus oocytes and have used this theory to characterize the role of the gamma subunit in single-channel behavior. Permeation and gating were found to be largely unaffected in AChRs produced in oocytes when the gamma subunit transcript was omitted from microinjections of AChR subunit RNAs. In contrast, marked changes in gating kinetics resulted when even very conservative single amino acid substitutions were introduced into the gamma subunit, indicating that the gamma subunit can have a large effect on AChR gating. We also found that channel openings were much prolonged when murine AChRs were expressed in BC3H-1 cells.
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Section of Molecular Neurobiology, Yale School of Medicine, New Haven, Connecticut 06510.
Pinkham
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Stevens
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D000818:Animals; D004553:Electric Conductivity; D004594:Electrophysiology; D005260:Female; D015870:Gene Expression; D015640:Ion Channel Gating / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology*; D007700:Kinetics; D046911:Macromolecular Substances; D008564:Membrane Potentials; D051379:Mice; D009154:Mutation; D009865:Oocytes / Q000378:metabolism; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D013329:Structure-Activity Relationship; D014162:Transfection; D014982:Xenopus laevis
D007473:Ion Channels; D046911:Macromolecular Substances; D012333:RNA, Messenger; D011978:Receptors, Nicotinic