Oxidant-induced restriction polymorphism maps to kinase region of c-abl oncogene.
Reactive oxygen species generated by activated human phagocytes can cause a variety of genetic injuries and produce malignant transformation in target cells. We previously reported that DNA extracted from phagocyte-transformed 10T1/2 mouse fibroblasts contained Msp I-dependent restriction fragment length polymorphisms in the c-abl oncogene. The data suggested that the oxidant-induced RFLP resulted from an alteration in the methylation pattern in c-abl. We have now mapped one of these RFLP to a specific 'CCGG' tetramer found within the tyrosine kinase region of the gene. The polymorphic 'CCGG' site has been localized to the intron between exon 2 and 3a. Restriction analysis indicates that a repetitive sequence exists within this intron and that the RFLP is associated with this repeat.
Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611.
C J
CJ
Schmeichel
S A
SA
Weitzman
D000818:Animals; D016313:Genes, abl / Q000235:genetics*; D010100:Oxygen / Q000502:physiology*; D012150:Polymorphism, Restriction Fragment Length; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D015183:Restriction Mapping
D011505:Protein-Tyrosine Kinases; D010100:Oxygen