Identification of B epitopes in human papillomavirus type 16 E7 open reading frame protein.
Human papillomavirus (HPV) type 16 is implicated in the aetiology of anogenital dysplasia which may progress to malignancy. HPV-16 DNA is actively transcribed in cervical carcinomas, the most abundant transcripts being from the E6 and E7 early open reading frames. The E7 protein has been shown to have transforming activity in vitro. In this report we define four immunodominant B epitopes within the protein corresponding to the E7 gene, using a panel of murine monoclonal antibodies. Three epitopes are linear and lie within the N-terminal region of the molecule, and are unique to the HPV-16 E7 protein. One epitope is non-linear and presumed to be conformational. At least three of the four epitopes of the E7 protein are detectable by immunoprecipitation from an HPV-16-infected cervical carcinoma cell line. The demonstrated immunogenicity of the E7 protein allows us to deduce that this molecule may be a potential candidate for incorporation in a vaccine against cervical cancer.
Department of Medicine, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
R W
RW
Tindle
J A
JA
Smith
H M
HM
Geysen
L A
LA
Selvey
I H
IH
Frazer
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology*; D001667:Binding, Competitive; D002460:Cell Line; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology; D009856:Oncogene Proteins, Viral / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology*; D050725:Papillomavirus E7 Proteins; D011487:Protein Conformation
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D009842:Oligopeptides; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16