Monocyte-T-cell interactions in pokeweed mitogen-activated cultures.
Monocyte-T-cell interactions were studied in pokeweed mitogen (PWM)-activated cell cultures. We addressed the question of monocyte changes in PWM-stimulated cultures of T cells and monocytes and found, by flow cytometric analysis, that PWM activation led to a loss of cells with monocyte or macrophage phenotype (CD14, HLA-DR, HLA-DQ) within 48 hr of culture in the presence of T cells (CD4+ T cells), but not in cultures of pure monocytes. Chemiluminescence measurements revealed that phagocytic stimulation of monocytic superoxide release was impaired in PWM-stimulated cultures of monocytes plus T cells, but not in PWM-stimulated cultures of pure monocytes. Furthermore, PWM induced the secretion of interferon-gamma (IFN-gamma) in primary cultures of T cells supplemented with 20% of monocytes, whereas in subsequent secondary cultures of these cells PWM induced IFN-gamma only when monocytes were added. We conclude from these flow cytometric and functional analyses that monocytes are efficiently eliminated from PWM-activated T-cell/monocyte cultures by CD4+ T lymphocytes.
Department of Clinical Immunology, Institute of Pediatrics, Cracow, Poland.
J
J
Pryjma
M
M
Zembala
A
A
Pituch-Noworolska
M
M
Ernst
J
J
Van der Bosch
H D
HD
Flad
D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D016827:CD8 Antigens; D002450:Cell Communication / Q000276:immunology; D002478:Cells, Cultured; D006681:HLA-D Antigens / Q000032:analysis; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D018950:Lipopolysaccharide Receptors; D008163:Luminescent Measurements; D009000:Monocytes / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D015214:Antigens, Differentiation, Myelomonocytic; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D006681:HLA-D Antigens; D018950:Lipopolysaccharide Receptors; D011043:Pokeweed Mitogens; D007371:Interferon-gamma