Identification and cDNA sequence of delta-preprotachykinin, a fourth splicing variant of the rat substance P precursor.
The neuropeptides substance P and neurokinin A are synthesised from a family of precursor polypeptides encoded by the preprotachykinin A (PPT) gene. In addition to a mRNA (beta-PPT) containing all 7 exons of the gene, alternatively spliced mRNAs lacking either exon 4 (gamma-PPT) or exon 6 (alpha-PPT) have been identified. We have determined the sequences of cDNA clones encoding four variants of PPT mRNA from rat dorsal root ganglion (DRG), including a novel mRNA species (delta-PPT) in which both exons 4 and 6 are absent. The sequence of delta-PPT predicts the existence of a novel tachykinin precursor polypeptide.
MRC Brain Metabolism Unit, Royal Edinburgh Hospital, Morningside Park, UK.
A J
AJ
Harmar
V
V
Hyde
K
K
Chapman
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005727:Ganglia, Spinal; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000145:classification / Q000235:genetics*; D012326:RNA Splicing; D051381:Rats; D013373:Substance P / Q000235:genetics*; D015320:Tachykinins / Q000145:classification / Q000235:genetics*
D011498:Protein Precursors; D015320:Tachykinins; C044206:preprotachykinin; D013373:Substance P; D004247:DNA