A synthetic peptide substrate for selective assay of protein kinase C.
Among various phosphate acceptor proteins and peptides so far tested, a synthetic peptide having the sequence surrounding Ser(8) of myelin basic protein, Gln-Lys-Arg-Pro-Ser(8)-Gln-Arg-Ser-Lys-Tyr-Leu, (MBP4-14), is the most specific and convenient substrate which can be used for selective assay of protein kinase C. This peptide is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca2+/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts. The Km value is considerably low (7 microM) with a Vmax value of twice as much as that for H1 histone.
Department of Biochemistry, Kobe University School of Medicine, Japan.
I
I
Yasuda
A
A
Kishimoto
S
S
Tanaka
M
M
Tominaga
A
A
Sakurai
Y
Y
Nishizuka
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D047388:Casein Kinases; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008168:Lung / Q000201:enzymology; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D004676:Myelin Basic Protein / Q000138:chemical synthesis*; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013379:Substrate Specificity
D007527:Isoenzymes; D004676:Myelin Basic Protein; D009842:Oligopeptides; D011494:Protein Kinases; D047388:Casein Kinases; D011493:Protein Kinase C