Properties of GMP-140, an inducible granule membrane protein of platelets and endothelium.
GMP-140 is an integral membrane glycoprotein with an apparent Mr of 140,000 located in secretory granules of human platelets and endothelial cells. When these cells are stimulated, the protein is rapidly redistributed to the plasma membrane; therefore, monoclonal antibodies to GMP-140 are useful markers of activated platelets and endothelium. GMP-140 is cysteine-rich and heavily glycosylated. The cDNA-derived amino acid sequence indicates that it contains a number of modular domains that are likely to fold independently. Beginning at the N-terminus, these comprise a "lectin" domain, an "EGF" domain, nine tandem consensus repeats similar to those in complement-binding proteins, a transmembrane domain, and a cytoplasmic tail. Some cDNAs also predict variant forms of GMP-140, including a putative soluble form lacking the transmembrane domain that appears to arise from alternative splicing of mRNA. The domain organization of GMP-140 is strikingly similar to two other vascular cell surface structures: ELAM-1, a cytokine-inducible endothelial cell receptor that binds neutrophils, and a lymphocyte-homing receptor that mediates the adherence of lymphocytes to high endothelial venules of peripheral lymph nodes. These "selectins" constitute a new gene family of receptors with related structure and potentially related function.
St. Francis Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City 73104.
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D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000032:analysis*; D006801:Humans; D005810:Multigene Family; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D013347:Subcellular Fractions / Q000032:analysis
D000911:Antibodies, Monoclonal; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins