[Characterization of a T-cell subpopulation with a new monoclonal antibody BL-TSub/2].
The mab BL-TSub/2 binds to a majority (80%) of thymocytes. The antigen isolated from these cells has an apparent molecular weight of about 170 kDa. The mab BL-TSub/2 recognized antigen is expressed on approximately 50% of mature T cells in peripheral blood. The mab BL-TSub/2 does not bind to monocytes, granulocytes, platelets and CD16+NK cells. The antigen is possibly present on a part of peripheral B cells in very low density. Double labelling experiments showed that about 30% of CD4+ and 60% of CD8+ cells react with the mab BL-TSub/2. This BL-TSub/2+ subset is not identical with T-cell subpopulations till now defined. Within the CD4+ subset the majority of BL-TSub/2+ cells is included in the CD45RO+ subset while only a small proportion is CD45RA+. However, the CD8+ BL-TSub/2+ cells are mostly CD45RA+ with only few cells belonging to the CD45RO+ subset.
Lehrstuhl für Immunologie, Universität Leipzig, Bundesrepublik Deutschland.
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D
Hamann
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H
Kupper
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W
Eichler
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Aust
H
H
Fiebig
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D017493:Leukocyte Common Antigens; D008970:Molecular Weight; D014066:Palatine Tonsil / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D006649:Histocompatibility Antigens; D017493:Leukocyte Common Antigens
Charakterisierung einer T-Zellsubpopulation durch einen neuen monoklonalen Antikörper BL-TSub/2.