Conserved epitopes of simian herpesvirus SA 8 and bovine herpesvirus type 2.
Some major structural components of simian herpesvirus SA 8 were analyzed and the relationship of SA 8 with HSV-1 and especially with BHV-2 was further characterized using a panel of SA 8- and BHV-2-specific monoclonal antibodies directed against gB, gD, gE, and ICP 8. It could be shown that SA 8 and BHV-2 expressed gB-1 equivalents, which differ in electrophoretic mobility, but share common epitopes with HSV-1. The antigenic determinants were detectable in the cytoplasm, on the surface of infected cells and on the virus envelope. Monoclonal antibodies reactive with epitopes of gB-SA 8 and gB-BHV-2 neutralized the homologous virus and cross-neutralized only HSV-1 and HSV-2, suggesting differences in accessibility of the corresponding epitope on SA 8 and BHV-2, respectively. A second protein with conserved epitopes on SA 8, BHV-2, and HSV-1 was identified as ICP 8. This nucleus associated protein was additionally detected on the envelope of SA 8 and HSV-1. The results imply that ICP 8 might have a function not only in virus replication, but also in virus assembly. We could furthermore define type-specific epitopes on two SA 8 envelope proteins which are analogous to gD-1 and gE-1, respectively. The gD-specific epitope induced a type-specific neutralizing antibody, making it interesting for differentiation of closely related herpesviruses.
Institut für Virologie, Freien Universität Berlin.
K
K
Borchers
M
M
Ozel
G
G
Pauli
H R
HR
Gelderblom
H
H
Ludwig
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000276:immunology*; D015151:Immunoblotting; D009500:Neutralization Tests; D011233:Precipitin Tests; D013045:Species Specificity; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D014764:Viral Proteins