[The immunofluorescent detection of phosphorylated receptors of the epidermal growth factor during their internalization in A-431 cells].
Phosphorylated receptors of the epidermal growth factor (EGF) were localized in the human epidermoid carcinoma cells using immunofluorescent staining with antibody to phosphotyrosine. The application of EGF at 4 degrees C was seen to induce a characteristic fluorescence of the cell margins, whereas no cell staining occurs in the absence of EGF. After a 1 hour incubation of cells at 37 degrees C, within which the internalized EGF receptor complexes are accumulated in the juxtanuclear compartment near the para-Golgi region, the staining with antiphosphotyrosine antibody reveals the receptors in this region. It is concluded that the internalized EGF-receptor complexes may remain in the phosphorylated state.
Vinogradova
N A
NA
Nesterov
A M
AM
Reshetnikova
G F
GF
Sorkin
A D
AD
Nikol'skiĭ
N N
NN
D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000032:analysis* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010766:Phosphorylation; D019000:Phosphotyrosine; D013194:Staining and Labeling / Q000379:methods; D013696:Temperature; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000276:immunology
D019000:Phosphotyrosine; D014443:Tyrosine; D004815:Epidermal Growth Factor; D066246:ErbB Receptors
Immunofluorestsentnoe vyiavlenie fosforilirovannykh retseptorov épidermal'nogo faktora rosta v protsesse ikh internalizatsii v kletkakh A-431.