[Immunohistochemical study of Y hapten and carcinoembryonic antigen in rectal carcinoma].
The monoclonal antibodies C14 and C365 which define the Y hapten and specific carcinoembryonic antigen (CEA) were shown by ABC-immunohistochemical technique to stain positively the tissue sections in 48 of 56 cases (85.7%) of rectal carcinoma for Y hapten, in 51 cases (91%) for CEA, and 100% for both together. Cancer cells which expressed Y hapten were mainly distributed in the foreland and deep invading cancer tissue which showed high ability of malignant growth. Two patterns of localization on the cancer cells for Y hapten were found: local distribution in cytoplasmic Golgi region, similar to blood group antigens, and diffused distribution on the membrane and in the cytoplasm as CEA. Positive expression of Y hapten on the carcinoma associated with types of differentiation and Duke's pathology stages: highly expressed on the poorly differentiated cancer and in Duke's A and C stages (P less than 0.05). Phenotypes of Y hapten and CEA on metastatic cancer cells in lymph nodes and primary cancers were similar. Our findings indicate that the antibodies may be useful in immunodiagnosis and immunotherapy.
Cancer Institute, China Medical University, Shenyang, Liao ning.
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Yang
D002288:Adenocarcinoma, Mucinous / Q000276:immunology / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002272:Carcinoembryonic Antigen / Q000378:metabolism*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016256:Lewis X Antigen / Q000378:metabolism*; D012004:Rectal Neoplasms / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D016256:Lewis X Antigen