Chemiluminescence response of human polymorphonuclear leukocytes induced by purified, latex attached Klebsiella fimbriae.
Type 1 fimbriae (T1F) and type 3 fimbriae (T3F) were isolated from Klebsiella species, purified, attached to latex beads and tested for their ability to stimulate human polymorphonuclear leukocyte (PMNL) oxidative activity. The luminol dependent chemiluminescence assay was used to evaluate the response of phagocytes. Latex particles coated with type 3 fimbriae (1-T3F) induced a significantly higher chemiluminescence response than those with type 1 fimbriae (1-T1F). Opsonization of 1-T1F with pooled human serum induced chemiluminescence responses which were statistically significantly enhanced as compared to opsonized 1-T3F and both kinds of non-opsonized fimbriae.
Institute of Medical Microbiology and Hygiene, University of Cologne.
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Przondo-Mordarska
H L
HL
Ko
J
J
Beuth
A
A
Gamian
G
G
Pulverer
D010861:Fimbriae, Bacterial / Q000276:immunology*; D006801:Humans; D007709:Klebsiella / Q000276:immunology* / Q000648:ultrastructure; D007711:Klebsiella pneumoniae / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008863:Microspheres; D009504:Neutrophils / Q000276:immunology* / Q000382:microbiology; D009895:Opsonin Proteins / Q000276:immunology; D010084:Oxidation-Reduction
D009895:Opsonin Proteins