HLA-DRw11, DQw7 has four cellular subtypes revealed by homozygous typing cells and undetected by restriction fragment length polymorphism.
HLA-DRw11 is in strong linkage disequilibrium with DQw7. We have investigated the heterogeneity of DRw11, DQw7 by cellular typing and by HLA cDNA probes. The results obtained by local and other homozygous typing cells in four informative Caucasoid families and 20 genotyped individuals demonstrate the existence of at least four different cellular subtypes. The frequency of DRw11, DQw7 is 25.7% with the following distribution of subtypes: Dw5 (17.3%), JAC (4.2%), JVM (2.8%), TIS (0.7%), and blank (0.7%). JVM (10W 9039) has been previously described; TIS (10W 9042) and JAC are postulated new specificities from our laboratory. None of these subtypes typed for DRw11, DQw1 cells. The cellular heterogeneity contrasts with the absence of polymorphism observed by serology and by restriction fragment length polymorphism after hybridization with DRB, DQA, and DQB probes. Variation in amino acids of the DRB1 chain has been reported for at least three variants: Dw5, JVM, and TIS (more recently).
Histocompatibility Laboratory, Blood Transfusion Centre, Lyon, France.
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AC
Freidel
L
L
Gebuhrer
H
H
Betuel
A
A
Farre
M P
MP
Labonne
J
J
Lambert
D000595:Amino Acid Sequence; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage; D006683:HLA-DQ Antigens / Q000145:classification* / Q000235:genetics; D006684:HLA-DR Antigens / Q000145:classification* / Q000235:genetics; D059810:HLA-DR Serological Subtypes; D006650:Histocompatibility Testing; D006801:Humans; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
D006683:HLA-DQ Antigens; C069546:HLA-DQ7 antigen; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C080766:HLA-DR11 antigen; D004247:DNA