Expression, function and regulation of the intercellular adhesion molecule-1 (ICAM-1) on human intestinal epithelial cell lines.
We have characterized the presence of the intercellular adhesion molecule-1 (ICAM-1) (CD54) on human intestinal adenocarcinoma cell lines as a nonreducible polypeptide of Mr 93 kDa, identified as a rhinovirus receptor. Expression of ICAM-1 was positively correlated with enterocytic maturation, in that the percentage of ICAM-1+ cells was highest in the most differentiated cell line Caco-2. ICAM-1 could be up-regulated only on the less differentiated cell lines HT29 and T84 by phorbol 12-myristate 13-acetate and by the cytokines interferon-gamma (IFN-gamma) and interleukin (IL) 1 beta. Enterocyte ICAM-1 was involved in adhesion to activated T cells through binding to the leukocyte function associated antigen-1 (LFA-1). These data provide evidence that colon adenocarcinoma cell lines express functional ICAM-1 sensitive to cytokine regulation. These findings support the hypothesis that lympho-epithelial interactions involving the ICAM-1/LFA-1 pathway may be implicated in immunosurveillance of colon adenocarcinomas, inflammatory bowel disease and celiac disease, where increased levels of proinflammatory cytokines are locally produced within the gut mucosa.
INSERM U 80, CNRS URA 1177 UCBL, Hôpital E. Herriot, Lyon, France.
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Kaiserlian
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Rigal
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Abello
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JP
Revillard
D015703:Antigens, CD / Q000378:metabolism; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1; D007413:Intestinal Mucosa / Q000378:metabolism*; D008854:Microscopy, Electron; D009479:Neuropeptides / Q000494:pharmacology; D011233:Precipitin Tests; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D016207:Cytokines; D009479:Neuropeptides; D018799:Intercellular Adhesion Molecule-1; D013755:Tetradecanoylphorbol Acetate