Nucleoid sedimentation analysis of DNA superstructure, gamma-radiation-induced damage and repair in human and chacma baboon (Papia ursinus) peripheral lymphocytes.
The use of the chacma baboon (Papio ursinus) in radiobiological investigations justifies special attention because it answers many of the criteria of parallelism to the human. The present study was undertaken to establish whether in vitro gamma-radiation effects in chacma baboon and human lymphocytes are comparable. The sensitive and rapid nucleoid sedimentation technique was employed to evaluate in vitro DNA superstructure, damage and repair in readily obtainable radiosensitive peripheral lymphocytes. Dose-response curves after 60Co gamma-irradiation were obtained, and by applying single-hit kinetics of the target theory, an estimation of molecular masses of the supercoiled domains was made. The baboon and human lymphocytes produced analogous results, while an ethidium bromide intercalation study also revealed a similarity in average DNA superhelical density. Lymphocyte DNA repair after 0.5-4.0 Gy gamma-irradiation and repair times from 0.5 to 5.0 h were evaluated. The repair data obtained from baboon and human cells after 2.0 Gy irradiation compared favourably in extent of DNA repair as well as the profiles of the kinetic curves. These findings indicate that the chacma baboon would be a useful and relevant model for further in vivo radiobiological studies on lymphocytes. The effects of sedimentation conditions and advantages of using vertical-tube rotors in the nucleoid sedimentation technique are also discussed.
Atomic Energy Corporation of South Africa Limited, Pretoria.
W K
WK
Louw
E J
EJ
van Rensburg
H
H
Izatt
R I
RI
Engelbrecht
D000818:Animals; D002469:Cell Separation / Q000379:methods; D003037:Cobalt Radioisotopes; D004247:DNA / Q000032:analysis / Q000528:radiation effects* / Q000648:ultrastructure; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D010215:Papio; D014461:Ultracentrifugation
D003037:Cobalt Radioisotopes; D004247:DNA