Association of p62c-yes with polyomavirus middle T-antigen mutants correlates with transforming ability.
A number of mutants of polyomavirus middle T antigen (MTag) were constructed into replication-competent avian retroviruses. To assess the ability of these MTag variants to transform and to associate with the avian p60c-src and p62c-yes proto-oncogene products, we used these viruses to infect chicken embryo fibroblasts. We found that the ability of individual mutant MTags to associate with p62c-yes correlated well with the ability of these mutants to transform, as has been previously shown for the association of MTag with p60c-src. All transformation-competent mutant MTags retained the ability to complex with p62c-yes. Two transformation-defective mutants, RX67 and RX68, which could weakly associate with p60c-src, were unable to associate with p62c-yes.dl1015, a transformation-defective mutant which could associate with p60c-src and with a phosphatidylinositol kinase activity, was also able to associate with p62c-yes. Therefore, some as yet unmeasured biochemical property is defective in this mutant.
Kornbluth
S
S
Laboratory of Molecular Oncology, Rockefeller University, New York, New York 10021-6399.
Cheng
S H
SH
Markland
W
W
Fukui
Y
Y
Hanafusa
H
H
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002472:Cell Transformation, Viral / Q000276:immunology*; D002645:Chickens; D006391:Hemangioma / Q000382:microbiology; D009154:Mutation; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics*; D011485:Protein Binding; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051575:Proto-Oncogene Proteins c-yes; D016392:Proto-Oncogene Proteins pp60(c-src); D014162:Transfection; D019061:src-Family Kinases
D000952:Antigens, Polyomavirus Transforming; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D051575:Proto-Oncogene Proteins c-yes; D016392:Proto-Oncogene Proteins pp60(c-src); D019061:src-Family Kinases