Organization of midbrain catecholamine-containing nuclei and their projections to the striatum in the North American opossum, Didelphis virginiana.
Presumptive catecholamine (CA) neurons in the opossum midbrain were identified by tyrosine hydroxylase immunohistochemistry. In the midline, small to moderate number of CA cells were present in the rostral third of the nucleus raphe dorsalis and throughout the nucleus linearis. Ventrolaterally, such cells were observed in the deep tegmental reticular formation, in all subnuclei of the ventral tegmental area, and in the three subdivisions of the substantia nigra. The CA cells in these areas conform to the dopamine cell groups, A8, A9, and A10 as described in the rat. In several areas there appeared to be no separation between the CA neurons belonging to cytoarchitecturally different nuclei. In order to determine which CA neurons gave rise to striatal projections, the neostriatum was injected with True Blue (TB), and sections through the midbrain were processed for tyrosine hydroxylase (TH) and visualized by immunofluorescence. Neurons containing both TB and TH were observed in each of the CA cell groups mentioned above. The distribution of these cells confirmed organizational features that may be unique to the opossum's substantia nigra. In addition, different patterns of labeling resulted from caudate versus putamen injections, suggesting a rudimentary medial to lateral topography in the organization of nigrostriatal projections. Although our results suggest that the organization of midbrain CA neurons in the opossum is similar to that in placental mammals, it is clear that differences exist.
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201.
J C
JC
Hazlett
R H
RH
Ho
G F
GF
Martin
D000818:Animals; D001931:Brain Mapping; D002395:Catecholamines / Q000502:physiology*; D003342:Corpus Striatum / Q000033:anatomy & histology* / Q000201:enzymology; D008636:Mesencephalon / Q000033:anatomy & histology* / Q000201:enzymology; D009474:Neurons / Q000201:enzymology; D009893:Opossums / Q000033:anatomy & histology*; D012154:Reticular Formation / Q000033:anatomy & histology / Q000201:enzymology; D013045:Species Specificity; D013378:Substantia Nigra / Q000033:anatomy & histology / Q000201:enzymology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D002395:Catecholamines; D014446:Tyrosine 3-Monooxygenase