[The study of Bacillus sphaericus Ts-1 protoplast-plasmid transformation of electroporation].
This report gave the best conditions of Bacillus sphaericus Ts-1 protoplast-plasmid pHV33 electroporation. The highest transformation frequency and transformation efficiency induced by three pulse of 21 KV/cm and 10 microseconds duration applied at an interval of one sec., was 2.44 x 10(2) transformants/micrograms DNA and 3.16 x 10(-6) respectively. The saturated concentration of DNA absorbed by the protoplast was 5 micrograms DNA/10(9) cells/ml. By means of this method, pJB417, a recombinant mosquito larvicide clone, was introduced into B. subtilis 168M and B. sphaericus Ts-1. The transformants of B. subtilis 168M with biocide activity were obtained, but the toxicity of B. sphaericus Ts-1 was not increased.
Department of Biology, Nankai University, Tianjin.
Y
Y
Wang
B
B
Jiang
R
R
Jiao
D000818:Animals; D001407:Bacillus / Q000235:genetics*; D009033:Culicidae; D004558:Electric Stimulation; D007814:Larva; D010572:Pest Control, Biological; D010957:Plasmids; D014169:Transformation, Bacterial