Insulin alters cell proliferation during the early development of rodent kidney.
The effects of insulin on early differentiated 15-day fetal mouse kidneys were assessed using an organotypic culture system. High concentrations (30 to 125 mU/ml) of the hormone drastically reduced (50%) the incorporation of 3H-thymidine in replicating cells without affecting either differentiation of forming nephrons or epithelio-mesenchymal relationships. When compared to insulin-like growth factor-I or the potent phorbol ester PMA, the action of insulin seemed to specifically deregulate some components of the transductional machinery controlling cell proliferation. This is opposed to the previous demonstration of a positive influence of insulin on cell proliferation in the human fetal kidney. The results suggest that the common definition of insulin as a fetal growth promoter may depend on the developmental stage of each organ, particularly for the mammalian kidney.
Département d'anatomie et de biologie cellulaire, Université de Sherbrooke, Québec, Canada.
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Chailler
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Brière
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D005333:Fetus / Q000166:cytology / Q000187:drug effects*; D007328:Insulin / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007668:Kidney / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008871:Microvilli / Q000201:enzymology; D009924:Organ Culture Techniques; D005723:gamma-Glutamyltransferase / Q000032:analysis
D007328:Insulin; D007334:Insulin-Like Growth Factor I; D004247:DNA; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase