Enzyme- and immuno-histochemical study of the thymic stroma in the rainbow trout, Salmo gairdneri, Richardson.
Owing to the lack of data about thymic non-lymphoid cells in fish we decided to perform a histochemical characterization of these cells in order to ascertain their relationships to other thymic components. In the present study we analyze the enzyme-histochemical patterns for acid phosphatase, alkaline phosphatase, non-specific sigma-naphthyl acetate esterase and 5' nucleotidase activities, as well as the presence of keratin demonstrated by immunoperoxidase staining, in the non-lymphoid cell populations of the thymus of the rainbow trout, Salmo gairdneri. According to their location in the organ, morphology and histochemical reactivities, we were able to define seven different subpopulations of keratin-positive epithelial cells: 1) Epithelial cells limiting with the capsular and septal connective tissues; 2) Subcapsular epithelial cells; 3) Stellate epithelial cells of the inner thymic zone; 4) Large, ovoid epithelial cells of the inner thymic zone; 5) Acidophilic epithelial cells of the outer thymic zone; 6) Cystic cells; and 7) Goblet cells. The significance of the heterogeneity of the epithelial cell (EC) population, its specific distribution in the organ, which apparently conforms distinct cell microenvironments, as well as the possible phylogenetical relationships between these microenvironments and the classical cortex and medulla of the mammalian thymus, are discussed.
Departamento de Biología Celular y Anatomía, Facultad de Biología, Universidad de León, Spain.
A
A
Castillo
B E
BE
Razquin
P
P
Lopez-Fierro
F
F
Alvarez
A
A
Zapata
A J
AJ
Villena
D015720:5'-Nucleotidase / Q000032:analysis; D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D004847:Epithelial Cells; D005260:Female; D006651:Histocytochemistry; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008297:Male; D009283:Naphthol AS D Esterase / Q000032:analysis; D012487:Salmonidae / Q000033:anatomy & histology*; D013950:Thymus Gland / Q000032:analysis / Q000033:anatomy & histology*; D014337:Trout / Q000033:anatomy & histology*
D007633:Keratins; D009283:Naphthol AS D Esterase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D015720:5'-Nucleotidase