Comparative study of phase II biotransformation in rabbit ocular tissues.
To assess the biotransformational capability of ocular tissues in the rabbit, representative phase II enzymes were assayed in five tissues from the eye, and in the liver, kidney, and intestine. Within the eye, the iris/ciliary body exhibited the highest glutathione S-transferase activity, whereas the cornea possessed the highest specific activities for N-acetyl-, sulfo-, and UDP-glucuronosyl-transferases. Cornea, iris/ciliary body, choroid, and retina exhibited significant activities of p-aminobenzoic acid N-acetyltransferase, 2-naphthol sulfotransferase, and 1-chloro-2,4-dinitrobenzene glutathione S-transferase. Despite its size and protein content, lens displayed little or no biotransformational activity. Only the iris/ciliary body conjugated sulfobromophthalein with glutathione. UDP-glucuronsyltransferase activity varied depending on tested substrates and tissues. When compared to liver, kidney, or intestine, N-acetyltransferase activity in the iris/ciliary body nearly matched the rate measured in kidney, glutathione S-transferase activity in cornea and iris/ciliary body was nearly 70 and 89%, respectively, of the rate in intestine, and corneal sulfotransferase activity was greater than that in kidney. These data suggest that biotransformation pathways are present in the eye, and particularly in ocular tissues having adequate blood supply or interfacing with the external environment.
Medical Sciences Program, Indiana University School of Medicine, Bloomington 47405.
J B
JB
Watkins
D P
DP
Wirthwein
R A
RA
Sanders
D000123:Acetyltransferases / Q000378:metabolism; D000818:Animals; D001711:Biotransformation; D003600:Cytosol / Q000378:metabolism; D005123:Eye / Q000201:enzymology / Q000378:metabolism*; D005136:Eye Proteins / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000378:metabolism; D011817:Rabbits; D015262:Xenobiotics / Q000378:metabolism*
D005136:Eye Proteins; D015262:Xenobiotics; D000123:Acetyltransferases; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase