Simultaneous assessment of blood flow in UVB-inflamed human skin by laser Doppler flowmetry and the 133-xenon washout technique.
The purpose of the study was to compare skin blood flow by laser Doppler flowmetry (LDF) and the 133-Xenon washout technique in UVB-inflamed human skin. Six healthy subjects participated in the study. Forearm skin blood flow was measured prior to irradiation and then 8, 24, 48 and 72 h after the exposure to twice the minimal erythema dose of UVB. Baseline blood flow as measured by the 133-Xenon washout method was 5.2 (range 3.0 to 10.4) ml/100g/min and LDF flux was 3.1 (range 2.7 to 5.7) arbitrary units. Following irradiation, maximum blood flow increase as evaluated by the 133-Xenon washout was 10.8 (95% confidence interval 3.7 to 11.3) times baseline blood flow, corresponding to an absolute blood flow of 47.5 (range 33.8 to 56.3) ml/100g/min. Maximum increase after induction of inflammation as assessed by LDF was 34.6 (95% confidence interval 24.6 to 56.5) times baseline flux. The two methods showed comparable time courses in all subjects. A significant correlation between the two methods was found, Spearman's rho = 0.54, p = 0.006. The relative LDF blood flow increase was 4.2-fold (95% confidence interval 2.7 to 5.0) greater than the increase measured by the 133-Xenon washout method. These results are at variance with previous comparative studies of the two methods. Some explanations are discussed.
Petersen
L J
LJ
Department of Clinical Physiology/Nuclear Medicine, Bispebjerg Hospital, University of Copenhagen, Denmark.
Kristensen
J K
JK
D000328:Adult; D005260:Female; D006801:Humans; D007834:Lasers; D008297:Male; D008875:Middle Aged; D010787:Photosensitivity Disorders / Q000000981:diagnostic imaging / Q000503:physiopathology*; D011877:Radionuclide Imaging; D012039:Regional Blood Flow; D012867:Skin / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000503:physiopathology; D014980:Xenon Radioisotopes
D014980:Xenon Radioisotopes