Substrate-dependent angiotensin II formation in the peripheral circulation.
An alternative angiotensin II-forming system distinct from the vascular renin-angiotensin system was demonstrated using a rat hindlimb perfusion system and synthetic substrates. This pathway was resistant to captopril and aprotinin, but was highly sensitive to chymostatin. Moreover, angiotensin II formation was substrate-dependent, i.e. angiotensin II formation from tridecapeptide human renin substrate in the presence of captopril was more than twice than that from an equimolar amount of angiotensin I. Both pathways may play a role in regulating the peripheral circulation.
Department of Internal Medicine, Fukuoka University School of Medicine, Japan.
M
M
Ideishi
M
M
Sasaguri
M
M
Ikeda
K
K
Arakawa
D000803:Angiotensin I / Q000378:metabolism; D000804:Angiotensin II / Q000096:biosynthesis / Q000097:blood*; D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D002216:Captopril / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000378:metabolism; D013379:Substrate Specificity
D009842:Oligopeptides; D000804:Angiotensin II; D000803:Angiotensin I; C002101:chymostatin; D007611:Aprotinin; D002216:Captopril; D002918:Chymotrypsin; D012083:Renin