Expression of multidrug-resistance (MDR1) gene in normal epithelia and in invasive carcinomas of the uterine cervix.
Total RNA from 92 invasive cervical cancers was analyzed for the presence of multidrug-resistance (MDR1) gene (also known as PGY1) transcripts. A 4.5-kilobase MDR1 transcript band was detected in 40 (43%) of 92 invasive cervical carcinomas and in 15 (68%) of 22 normal cervices. MDR1 levels were low [mean, 2.5 arbitrary units (U)] except in one liver metastasis (50 U) treated with a drug regimen including vincristine. Of eight carcinomas treated by radiotherapy and/or chemotherapy, seven (88%) exhibited MDR1 transcripts as compared with 24 (35%) of 69 untreated carcinomas (Fisher's exact test; P = .01). In conclusion, our data suggest that the MDR1 gene plays a role in drug resistance of certain cervical cancers, but also that other mechanisms may be involved.
Laboratoire de Pharmacologie Clinique et Moléculaire, Institute Gustave Roussy, Villejuif, France.
G F
GF
Riou
D
D
Zhou
J C
JC
Ahomadegbe
M
M
Gabillot
P
P
Duvillard
C
C
Lhomme
D000230:Adenocarcinoma / Q000235:genetics; D015152:Blotting, Northern; D002277:Carcinoma / Q000235:genetics*; D002278:Carcinoma in Situ / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002584:Cervix Uteri / Q000187:drug effects; D004351:Drug Resistance / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005796:Genes; D006801:Humans; D009367:Neoplasm Staging; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D012313:RNA