A single nucleotide substitution in the D domain of estrogen receptor cDNA causes amino acid alteration from Glu-279 to Lys-279 in a murine transformed Leydig cell line (B-1 F).
A polymerase chain reaction method was carried out to address the possible presence of estrogen receptor (ER) mutations in murine transformed cell lines. The segment of ER cDNA coding the C and D domains was amplified and cloned into pUC 19. Mammary carcinoma cell line (SC-3) did not show any mutation in this segment. However, the sequence analysis of ER in the Leydig cell line (B-1 F) revealed a single base change at acidic Glu-279 (GAA) to basic Lys-279 (AAA) compared with murine uterus ER cDNA. The biological significance of this mutation is discussed.
Third Department of Internal Medicine, Osaka University Hospital, Japan.
T
T
Hirose
M
M
Koga
K
K
Matsumoto
B
B
Sato
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002645:Chickens; D005971:Glutamates / Q000235:genetics*; D018698:Glutamic Acid; D006801:Humans; D007985:Leydig Cells; D008239:Lysine / Q000235:genetics*; D008297:Male; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000737:chemistry; D051381:Rats; D011960:Receptors, Estrogen / Q000737:chemistry / Q000235:genetics*; D014407:Tumor Cells, Cultured
D005971:Glutamates; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D018698:Glutamic Acid; D008239:Lysine