An electrophysiological study on the effect of neuropeptide Y on sympathetic neurotransmission in guinea pig vas deferens.
The effect of neuropeptide Y (NPY) on sympathetic neurotransmission to the guinea pig vas deferens was studied by recording pre- and postsynaptic electrical events with a suction electrode. When applied locally to the recording site by internal perfusion of the suction electrode, NPY inhibited both the nerve action potential (NAP) and the excitatory junction current (EJC). When applied only to the outside bathing solution, NPY had no inhibitory effect on the NAP or the EJC. The site of action of NPY is therefore at the presynaptic nerve terminal. The mode of action of NPY is different from those of B-HT 920, an alpha-2 adrenoceptor agonist, and omega-conotoxin, a neurotoxin that acts specifically on presynaptic calcium channels. Both of these agents inhibited the EJC without any significant effect on the NAP.
University of Ottawa Heart Institute, Canada.
D W
DW
Cheung
R
R
Dukkipati
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D001381:Azepines / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D003470:Culture Media; D004594:Electrophysiology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009478:Neuropeptide Y / Q000494:pharmacology* / Q000502:physiology; D010456:Peptides, Cyclic / Q000494:pharmacology; D013564:Sympathetic Nervous System / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects*; D014649:Vas Deferens / Q000187:drug effects / Q000294:innervation; D020960:omega-Conotoxins
D000316:Adrenergic alpha-Agonists; D001381:Azepines; D002121:Calcium Channel Blockers; D003470:Culture Media; D009478:Neuropeptide Y; D010456:Peptides, Cyclic; D020960:omega-Conotoxins; C053423:Conus magus toxin; C024763:talipexole