Molecular organization of the human Raf-1 promoter region.
A genomic DNA fragment containing the Raf-1 promoter region was isolated by using a cDNA extension clone. Nucleotide sequencing of genomic DNA clones, primer extension, and S1 nuclease assays have been used to identify the 5' ends of Raf-1 RNAs. Consistent with its ubiquitous expression, the Raf-1 promoter region had features of a housekeeping gene in that it was GC-rich (HTF-like), lacked TATA and CAAT boxes, and contained heterogeneous RNA start sites and four potential binding sites for the transcription factor SP1. In addition, an octamer motif (ATTTCAT), a potential binding site for the octamer family of transcription factors, was located at -734 base pairs. The Raf-1 promoter region drove reporter gene expression 30-fold over the promoterless reporter in Cos 7 cells.
Program Resources, Inc., Biological Carcinogenesis Development Program, Frederick, Maryland 21701.
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Beck
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Brennscheidt
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Sithanandam
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Cleveland
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Rapp
D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf; D011519:Proto-Oncogenes; D012313:RNA / Q000235:genetics; D012326:RNA Splicing; D012333:RNA, Messenger; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000201:enzymology
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D011505:Protein-Tyrosine Kinases; D019908:Proto-Oncogene Proteins c-raf