Lectin binding ability of B-chronic lymphocytic leukaemia cells.
The binding of five fluorescein-labelled lectins: peanut agglutinin (PNA), lentil agglutinin (LEN), soybean agglutinin (SBA), wheat germ agglutinin (WGA) and asparagus pea agglutinin (ASP) to human B-cell chronic lymphocytic leukaemia (B-CLL) and B lymphocytes of normal donors was studied. The specificity of the fluorescence was demonstrated by inhibition with appropriate saccharides. The proportion of B cells was estimated using anti-B cell monoclonal antibody. Both leukaemic and normal B cells showed the binding ability of all except of one (ASP) studied lectins. We have found the differences in surface carbohydrate patterns between B-CLL and normal B lymphocytes. B-CLL cells showed the considerably lower ability to bind SBA and slightly higher expression of PNA and LEN receptors in comparison to normal B cells. The analysis of WGA binding allowed for recognizing two groups of CLL patients: one with high and the second one with low WGA receptor expression. The double marker studies revealed that B cells could simultaneously react with anti-B cell monoclonal antibody and fluorochrome labelled lectins.
Slesak
B
B
Department of Tumor Immunology, Medical University, Wroclaw/Poland.
Harłozińska-Szmyrka
A
A
Frydecka
I
I
D001402:B-Lymphocytes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000378:metabolism*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism*; D019887:Peanut Agglutinin; D010835:Phytohemagglutinins / Q000378:metabolism; D037121:Plant Lectins; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D037102:Lectins; D019887:Peanut Agglutinin; D010835:Phytohemagglutinins; D037121:Plant Lectins; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C033619:lentil lectin; C036292:pea lectin; C028824:soybean lectin