Adherence to HEp-2 cells and replication in macrophages of Salmonella derby of human origin.
Adherence to a HEp-2 cell monolayer was tested for in four strains of Salmonella derby which were isolated from patients with diarrhea. One strain, SB1, was highly adherent and another strain, SB4, was nonadherent. The other two strains exhibited moderate adherence. Further in vitro study of invasion of HEp-2 cells by S. derby and its replication in murine peritoneal macrophages was carried out using SB1 and SB4. Thin section electron micrographs revealed that SB1 invaded HEp-2 cells but SB4 did not. The number of viable bacteria within macrophages was determined at intervals after inoculation of bacteria. The result indicates that SB1 can replicate in the macrophages but SB4 cannot. Flagella and fimbriae were compared by electron microscopy between SB1 and SB4, and their lipopolysaccharides and outer membrane proteins were also compared with each other by SDS-polyacrylamide gel electrophoresis. The presence of a 41 kDa protein in the outer membranes of SB1 was only the difference detected, suggesting that this protein could be a factor required for adherence of this serovar to epithelial cells.
Budiarti
S
S
Department of Microbiology, Kagawa Medical School.
Hirai
Y
Y
Minami
J
J
Katayama
S
S
Shimizu
T
T
Okabe
A
A
D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D002460:Cell Line; D015169:Colony Count, Microbial; D003967:Diarrhea / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D010861:Fimbriae, Bacterial / Q000737:chemistry / Q000648:ultrastructure; D005407:Flagella / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial / Q000032:analysis; D012475:Salmonella / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins; D011135:Polysaccharides, Bacterial