1 alpha,25-dihydroxyvitamin D3 inhibits productive infection of human monocytes by HIV-1.
Pretreatment of freshly isolated human peripheral blood monocytes with the steroid hormone, 1 alpha,25-dihydroxyvitamin D3 (1,25(OH)D), markedly reduced (by 95%) productive infection of human monocytes by HIV-1. Equivalent concentrations (10nM) of 25-hydroxyvitamin D3 (25(OH)D), the biologic precursor of 1,25(OH)D, were ineffective at reducing either CD4 expression or HIV-1 production. Pretreatment was required for modulation of HIV-1 infection by 1,25(OH)D. Interestingly, 1,25(OH)D-mediated decreases in p24 antigen production were observed prior to any observed reduction in CD4 expression, suggesting that 1,25(OH)D treatment may modulate HIV-1 infection of monocytes through additional factors besides decreased HIV-1 binding. These data raise the possibility that 1,25(OH)D compounds may be important in host resistance to HIV-1.
Connor
R I
RI
Department of Microbiology, Dartmouth Medical School, Hanover, NH 03756.
Rigby
W F
WF
D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000382:microbiology; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D015683:Gene Products, gag / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D009000:Monocytes / Q000187:drug effects / Q000382:microbiology*; D014758:Viral Core Proteins / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects
D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D014758:Viral Core Proteins; D002117:Calcitriol