Interferon induction by viruses. XX. Acid-labile interferon accounts for the antiviral effect induced by poly(rI).poly(rC) in primary chick embryo cells.
Primary chick embryo cells which were "aged" in vitro and exposed to poly(rI).poly(rC) developed a disproportionately high degree of resistance to virus challenge in relation to the amount of conventional acid-stable interferon (IFN) produced. Cells were often highly resistant after exposure to a few nanograms of poly(rI).poly(rC) which gave rise to little or no acid-stable IFN. Study of the mechanism(s) responsible showed that this antiviral state was not due to the double-stranded ribonuclease induced in parallel, nor to residual poly(rI).poly(rC) (which this ribonuclease destroyed), but to the production of a hitherto undescribed acid-labile chicken IFN.
Yoshida
I
I
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-3044.
Marcus
P I
PI
D000143:Acids; D000818:Animals; D001681:Biological Assay; D002478:Cells, Cultured; D002642:Chick Embryo; D004722:Endoribonucleases / Q000032:analysis / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects; D007372:Interferons / Q000096:biosynthesis*; D011070:Poly I-C / Q000494:pharmacology*; D012330:RNA, Double-Stranded / Q000494:pharmacology; D043244:Ribonuclease III; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*
D000143:Acids; D012330:RNA, Double-Stranded; D007372:Interferons; D004722:Endoribonucleases; D043244:Ribonuclease III; D011070:Poly I-C