A simple method for direct cloning cDNA sequence that flanks a region of known sequence from total RNA by applying the inverse polymerase chain reaction.
Division of Hematology/Oncology, Children Hospital of Los Angeles, CA 90054.
S H
SH
Huang
Y Y
YY
Hu
C H
CH
Wu
J
J
Holcenberg
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics*; D003842:Deoxycytidine Kinase / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000235:genetics
D015345:Oligonucleotide Probes; D012313:RNA; D004247:DNA; D003842:Deoxycytidine Kinase