Karyometric marker features in fine needle aspirates of microinvasive follicular carcinoma of the thyroid.
Karyometric measurements were performed on fine needle aspirates of clearly identifiable tumor areas and adjacent normal-appearing areas in the surgical specimens from ten patients with microinvasive follicular carcinoma of the thyroid. Similar measurements were performed on aspirates from nine patients free of thyroid disease (controls). A total of 95 karyometric features were evaluated for each nucleus. As compared with the control nuclei, the normal-appearing nuclei showed a 6% increase in total nuclear optical density (OD) while the tumor nuclei showed a 14% increase. Analysis of variance indicated a significant difference between the normal-appearing nuclei and the control nuclei, with most of the difference due to the differences of tissue origin. Discriminant analysis selected nine features that produced a statistically highly significant separation of tumor nuclei from control nuclei. A similar analysis selected five features that produced a statistically highly significant discrimination of normal-appearing nuclei from control nuclei; the validity of those karyometric features as markers of malignancy in normal-appearing nuclei from tissues adjacent to microinvasive follicular carcinomas of the thyroid was demonstrated by analysis in further training and test sets of nuclei.
Bibbo
M
M
Department of Obstetrics and Gynecology, University of Chicago, IL 60637.
Bartels
P H
PH
Salguero
M
M
Dytch
H E
HE
Lerma-Puertas
E
E
Galera-Davidson
H
H
D000230:Adenocarcinoma / Q000032:analysis / Q000648:ultrastructure*; D001707:Biopsy, Needle; D002467:Cell Nucleus / Q000032:analysis*; D004396:Coloring Agents; D006801:Humans; D007620:Karyometry; D011003:Ploidies; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D013964:Thyroid Neoplasms / Q000032:analysis / Q000648:ultrastructure*
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes