Isolation of coordinately regulated genes that are expressed in discrete stages of B-cell development.
We have utilized subtractive hybridization to isolate 16 distinct cDNA sequences representing genes expressed in pre-B-cell lines but not myeloma cell or fibroblast lines. These sequences represent RNA transcripts that vary in abundance in pre-B-cell lines from 0.001% to 0.05%. Five of these sequences were not related to any known genes. One was related to but distinct from known myosin regulatory light chain genes and another encoded a protein with lectin domains. Three represented previously identified genes encoding carbonic anhydrase type II, thymosin, and CD2; these genes were not previously known to be specifically expressed in early stages of B-cell development. Other isolated genes corresponded to pre-B-cell-specific or pre-B-cell/B cell-specific genes recently described by others. The isolated cDNA sequences may be divided into two general categories--those representing genes expressed only in the pre-B-cell stage of B-cell development and those expressed in both the pre-B-cell and B-cell stages. The in vivo expression patterns of the identified genes suggest that some function specifically in lymphocytes while others may have roles in additional lineages.
Yancopoulos
G D
GD
Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
Oltz
E M
EM
Rathbun
G
G
Berman
J E
JE
Smith
R K
RK
Lansford
R D
RD
Rothman
P
P
Okada
A
A
Lee
G
G
Morrow
M
M
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005786:Gene Expression Regulation; D015723:Gene Library; D007739:L Cells / Q000276:immunology; D051379:Mice; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D015342:DNA Probes; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA