Secondary in vitro B lymphocyte (antibody) response to microbial antigens: use in appraisal of vaccine immunogenicity and cytokine immunoregulation.
In order to perform preliminary evaluations of subunit vaccine candidates before extensive trials in large food-producing animals, an in vitro B-lymphocyte response assay, based on the principles of an ELISA, was established. The assay was developed in detail for the porcine system using antigens from the Gram-negative bacterium Actinobacillus pleuropneumoniae, but is shown to be applicable to other species and antigens, including viral components. It is further shown that B-cell activity in the assay is dependent on T-helper cells as well as macrophages and/or their secretory products. Thus, in addition to providing a tool for evaluation of T and B memory cell activity, the system also lends itself to dissection of T-B cell collaboration and the regulatory functions of interleukins in secondary (in vitro) antibody responses.
Veterinary Infectious Disease Organization, Saskatoon, Canada.
H B
HB
Ohmann
L
L
McDougall
A
A
Potter
D000188:Actinobacillus / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007242:Herpesvirus 1, Bovine / Q000276:immunology; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine; D014611:Vaccination
D000907:Antibodies, Bacterial; D000956:Antigens, Viral; D001428:Bacterial Vaccines; D007376:Interleukin-2; D011994:Recombinant Proteins; D007371:Interferon-gamma