Effect of UV-irradiation on rotavirus.
The effect of UV-irradiation on SAll rotavirus infectivity was followed. The time course of infectivity inactivation in general showed an one-hit pattern. Two basic effects of UV-irradiation on virus particles were investigated: the phenomenon of RNA-protein linkages and the formation of uracil dimers. To determine the number of uridine dimers, 3H-uridine labelled purified rotavirus was exposed to UV-irradiation, subsequently the RNA was extracted and analysed by ascending paper chromatography. Formation of photodimers was found to be an important mechanism of rotavirus inactivation at conventional UV-irradiation; the RNA-protein linkages were registered at high irradiation doses only.
D. I. Ivanovsky Institute of Virology of the U.S.S.R. AMS, Moscow.
Y A
YA
Smirnov
S P
SP
Kapitulets
N N
NN
Amitina
V A
VA
Ginevskaya
N V
NV
Kaverin
D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004307:Dose-Response Relationship, Radiation; D011740:Pyrimidine Dimers; D012330:RNA, Double-Stranded / Q000528:radiation effects*; D012367:RNA, Viral / Q000528:radiation effects*; D012401:Rotavirus / Q000502:physiology / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014498:Uracil / Q000528:radiation effects; D014764:Viral Proteins / Q000528:radiation effects*
D011740:Pyrimidine Dimers; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D014498:Uracil