Effects of doxorubicin on maturation of human monocytes in adherent and non-adherent cultures.
Purified human monocytes were cultured for 2 h, 88 h, and 10 days in plastic tubes (adherent) and for 10 days in Teflon foil bags (non-adherent). Monocytes were incubated with doxorubicin by two short-term exposures (750 or 1500 ng/ml) for 1 h or by continuous exposure (75 ng/ml). Maturation was monitored by measuring the intracellular activity of three metabolic enzymes and two acid hydrolases. Expression of receptors for the Fc moiety of immunoglobulin G (FcRI, FcRII, FcRIII), CD14, and HLA-DR was assayed by indirect immunofluorescence with monoclonal antibodies. In the presence of doxorubicin, the adherent capacity, the yield, and the enzyme activities reflecting growth and intermediary metabolism were similar to the control groups. However, doxorubicin reduced the expression of FcRI (32-45%), FcRII (10-26%), CD14 (20-37%), and HLA-DR (25-34%) on the monocyte-derived macrophages. Expression of FcRIII was not detectable after 10 days of culture.
Division of Medical Oncology, University Hospital Nijmegen, The Netherlands.
R C
RC
van Schie
P H
PH
de Mulder
H
H
van Rennes
H G
HG
Verstraten
D J
DJ
Wagener
D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004317:Doxorubicin / Q000494:pharmacology*; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D018950:Lipopolysaccharide Receptors; D009000:Monocytes / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D011961:Receptors, Fc / Q000032:analysis; D013997:Time Factors
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D006684:HLA-DR Antigens; D018950:Lipopolysaccharide Receptors; D011961:Receptors, Fc; D004317:Doxorubicin