Alpha-2-macroglobulin is not synthesized by human urothelial cell lines in vitro.
We have analyzed 14 human urothelial cell lines in two different transformation grades for the production of a tumor-associated-alpha-2-macroglobulin. It has been shown in radioimmunoprecipitation and immunoblotting tests that human urothelial cells in vitro do not synthesize the wide-spectrum proteinase inhibitor alpha-2-macroglobulin. Four of the tested cell lines were additionally tested for the expression of specific mRNA which resulted in negative findings. The significance of the absence of alpha-2-macroglobulin in the human urothelial cells is discussed.
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
M
M
Grófová
J
J
Bízik
A
A
Lizonová
J
J
Kieler
D000818:Animals; D002471:Cell Transformation, Neoplastic; D006801:Humans; D008545:Melanoma / Q000378:metabolism; D009361:Neoplasm Invasiveness; D011233:Precipitin Tests; D011817:Rabbits; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000096:biosynthesis* / Q000276:immunology
D000511:alpha-Macroglobulins