Induction of cell-associated interleukin 1 through stimulation of the adhesion-promoting proteins LFA-1 (CD11a/CD18) and CR3 (CD11b/CD18) of human monocytes.
Serum-free culture of human monocytes in the presence of monoclonal antibodies to the LFA-1 alpha chain (CD11a), CR3 alpha chain (CD11b) or beta chain (CD18) bound to Sepharose induced the dose-dependent production of cell-associated interleukin (IL) 1 activity and of IL 1 alpha and IL 1 beta antigens, but no release of extracellular IL 1 activity or antigen in the culture medium. Triggering of IL 1 production was also observed with insolubilized anti-CD11/CD18 F(ab')2 antibodies. Two cross-linked antibodies recognizing distinct epitopes on the CD11b molecule induced cell-associated IL 1. Soluble antibodies did not induce IL 1 production. The kinetics of induction of IL 1 by stimulation of adhesion-promoting proteins differed from those of IL 1 induction by adhesion to plastic. The lack of induction of IL 1 release by stimulation of the CD11/CD18 molecules resembled the intracellular accumulation of IL 1 induced by lipid A. Induction of IL 1 by adhesive processes may be a mechanism by which T cells trigger IL 1 production by monocytes during antigen presentation.
INSERM U 28, Hôpital Broussais, Paris, France.
C
C
Couturier
N
N
Haeffner-Cavaillon
L
L
Weiss
E
E
Fischer
M D
MD
Kazatchkine
D000943:Antigens, Differentiation / Q000502:physiology*; D018821:CD18 Antigens; D002448:Cell Adhesion / Q000502:physiology; D000939:Epitopes; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D007700:Kinetics; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D009000:Monocytes / Q000276:immunology*; D010969:Plastics; D016028:Receptors, Leukocyte-Adhesion / Q000502:physiology*
D000943:Antigens, Differentiation; D018821:CD18 Antigens; D000939:Epitopes; D007375:Interleukin-1; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D010969:Plastics; D016028:Receptors, Leukocyte-Adhesion