Cytokine production by allergen (Der pI)-specific CD4+ T cell clones derived from a patient with severe atopic disease.
Twenty-four T cell clones (TCC) specific for purified Dermatophagoides pteronyssinus group I allergen (Der p I) were established from the peripheral blood of a patient with severe atopic disease and assessed for cytokine production in response to stimulation with both phorbol 12-myristate 13-acetate and anti-CD3 monoclonal antibody. All Der p I-specific TCC produced high amounts of interleukin (IL)-4 in association with IL-3, IL-5 and granulocyte monocyte-colony stimulating factor (GM-CSF), whereas they produced variable amounts of IL-2 and virtually no interferon-gamma. These data support the hypothesis that atopy is associated with preferential activation of type 2 T helper cells and suggest a deregulation in the function of the IL-3, IL-4, IL-5 and GM-CSF gene cluster in subjects with severe atopic disorders.
Immunologia Clinica e Allergologia, Università degli Studi di Firenze, Florence, Italy.
P
P
Parronchi
R
R
Manetti
C
C
Simonelli
F S
FS
Rugiu
M P
MP
Piccinni
E
E
Maggi
S
S
Romagnani
D000328:Adult; D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000276:immunology* / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002455:Cell Division; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008925:Mites / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism*
D000485:Allergens; D039741:Antigens, Dermatophagoides; D016207:Cytokines