Immortalization of primary rat embryo cells by human papillomavirus type 11 DNA is enhanced upon cotransfer of ras.
Transfer of human papillomavirus type 11 (HPV11) DNA and a neo(r) marker into primary rat embryo cells (REC) led to colony formation in G418-selective medium. About 20% of HPV11 clones were eventually established in culture but displayed low growth rates. Cotransfection of HPV11 DNA and an activated ras oncogene led to formation of both drug-resistant flat colonies and phenotypically transformed clones which grew efficiently when expanded in culture. A number of transformants reverted to a flat, "normal" morphology shortly after isolation. Nontransformed clones expressed only HPV11 genes, while those maintaining a transformed phenotype transcribed both ras and HPV11 genes efficiently and were highly tumorigenic. Expression of HPV11 thus seems, necessary for induction of colony formation, but efficient long-term growth seems to require at least the transient presence of ras.
Institute for Tumor Biology and Cancer Research, University of Vienna, Austria.
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Meneguzzi
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015152:Blotting, Northern; D015139:Blotting, Southern; D002455:Cell Division; D002472:Cell Transformation, Viral; D002999:Clone Cells; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004351:Drug Resistance / Q000235:genetics; D004622:Embryo, Mammalian; D005260:Female; D011905:Genes, ras; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D009374:Neoplasms, Experimental / Q000473:pathology; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection
D000900:Anti-Bacterial Agents; D004279:DNA, Viral; D005839:Gentamicins; D012313:RNA; C010680:antibiotic G 418