Nucleotide sequencing and characterization of Pseudomonas putida catR: a positive regulator of the catBC operon is a member of the LysR family.
Pseudomonas putida utilizes the catBC operon for growth on benzoate as a sole carbon source. This operon is positively regulated by the CatR protein, which is encoded from a gene divergently oriented from the catBC operon. The catR gene encodes a 32.2-kilodalton polypeptide that binds to the catBC promoter region in the presence or absence of the inducer cis-cis-muconate, as shown by gel retardation studies. However, the inducer is required for transcriptional activation of the catBC operon. The catR promoter has been localized to a 385-base-pair fragment by using the broad-host-range promoter-probe vector pKT240. This fragment also contains the catBC promoter whose -35 site is separated by only 36 nucleotides from the predicted CatR translational start. Dot blot analysis suggests that CatR binding to this dual promoter-control region, in addition to inducing the catBC operon, may also regulate its own expression. Data from a computer homology search using the predicted amino acid sequence of CatR, deduced from the DNA sequence, showed CatR to be a member of a large class of procaryotic regulatory proteins designated the LysR family. Striking homology was seen between CatR and a putative regulatory protein, TfdS.
Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago 60612.
R K
RK
Rothmel
T L
TL
Aldrich
J E
JE
Houghton
W M
WM
Coco
L N
LN
Ornston
A M
AM
Chakrabarty
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D001565:Benzoates / Q000378:metabolism*; D019748:Carbon-Carbon Double Bond Isomerases; D003001:Cloning, Molecular / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D019753:Intramolecular Lyases; D007535:Isomerases / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D014176:Protein Biosynthesis; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D001565:Benzoates; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D007535:Isomerases; D019748:Carbon-Carbon Double Bond Isomerases; C100087:muconolactone delta-isomerase; D019753:Intramolecular Lyases; C021811:muconate cycloisomerase