Determination of tertatolol enantiomers in biological fluids by high-performance liquid chromatography.
A stereospecific high-performance liquid chromatographic method for the quantification of (-)- and (+)-tertatolol in plasma and urine is described. The method involves solid-phase extraction followed by derivatization with S(+)-naphthylethylisocyanate to form the urea derivative, which is more sensitive to fluorescence detection. The separation of the diastereomeric derivatives was performed by reversed-phase high-performance liquid chromatography. Fluorimetric detection (lambda excitation = 220 nm, lambda emission = 320 nm) allows the quantification of tertatolol enantiomers down to 6 ng/ml. The assay was used to study the pharmacokinetic profile of tertatolol enantiomers following oral administration of racemic tertatolol; preliminary results suggest enantioselective absorption and/or disposition of tertatolol.
Faculté de Pharmacie, Laboratoire de Pharmacie Chimique, Illkirch-Graffenstaden, France.
T
T
Lave
C
C
Efthymiopoulos
J C
JC
Koffel
L
L
Jung
D000319:Adrenergic beta-Antagonists / Q000097:blood / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D011412:Propanolamines / Q000097:blood / Q000378:metabolism* / Q000652:urine; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D013876:Thiophenes
D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; D013876:Thiophenes; C005632:tertatolol