Differentiation of lactococci by rRNA gene restriction analysis.
Strains of the subspecies of Lactococcus lactis could be differentiated by rRNA gene restriction fragment length polymorphisms (RFLP). 16S rRNA-specific oligonucleotide as well as polynucleotide DNA probes were used for the detection of restriction fragments. In addition, a site-specific probe was designed for the intergenic spacer region of 23S and 5S rRNA genes. For all lactococcal strains the putative presence of six rRNA operons was confirmed. A non-radioactive hybridization assay was used based on hybrid detection by chemiluminescence. Specific patterns were found for any of the strains investigated. Subspecies-specific restriction fragments could be identified in addition to the strain-specific patterns.
Lehrstuhl für Mikrobiologie, Technische Universität München, F.R.G.
G
G
Köhler
W
W
Ludwig
K H
KH
Schleifer
D001483:Base Sequence; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D013294:Lactococcus lactis / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping
D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D015246:Deoxyribonuclease EcoRI