Structural features of the priming signal recognized by primase: mutational analysis of the phage G4 origin of complementary DNA strand synthesis.
45 mutations (insertion, deletion and base substitution) of the G4 Goric were tested for their functional activity in M13 and R199 in vivo. The critical mutants were also assayed for their ability to synthesize pRNA in vitro using SSB and primase. The results demonstrate that the secondary structure and spacing of stem-loops I and III are essential for Goric activity and that the 5'-CTG-3' sequence flanking stem-loop I is essential for initiation of pRNA synthesis.
Department of Agricultural Chemistry, Kyoto University, Japan.
H
H
Hiasa
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H
Sakai
T
T
Komano
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GN
Godson
D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004252:DNA Mutational Analysis; D019915:DNA Primase; D004261:DNA Replication; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000096:biosynthesis*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000096:biosynthesis; D012316:RNA Nucleotidyltransferases / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D004279:DNA, Viral; C034477:RNA primers; D012313:RNA; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases