The structure and expression of the guinea pig Fc receptor for IgG1 and IgG2 (Fc gamma 1/gamma 2R).
A cDNA clone encoding the receptor for guinea pig immunoglobulin G was isolated from a guinea pig peritoneal macrophage cDNA library. The cloned cDNA encoded 271 amino acids containing an N-terminal signal sequence. The deduced amino acid sequence is most homologous to murine Fc gamma RII beta 2. The receptor protein could be expressed in COS-7 and L cells transfected with the cDNA, suggesting that the expression of this receptor does not require the co-expression of a second chain such as gamma chain of Fc epsilon RI or CD3 zeta chain. The transformant L cells showed the binding to both the guinea pig IgG1 and IgG2 antibodies complexed with antigen, indicating that the cDNA we cloned was the one for guinea pig Fc gamma 1/gamma 2R.
Department of Biochemistry, Kumamoto University Medical School, Japan.
M
M
Tominaga
A
A
Sakata
T
T
Ohmura
T
T
Yamashita
J
J
Koyama
K
K
Onoue
D000595:Amino Acid Sequence; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000302:isolation & purification; D005260:Female; D015870:Gene Expression; D006168:Guinea Pigs; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis; D011961:Receptors, Fc / Q000235:genetics*; D017452:Receptors, IgG; D013329:Structure-Activity Relationship; D014162:Transfection
D000943:Antigens, Differentiation; D011961:Receptors, Fc; D017452:Receptors, IgG; D012313:RNA; D004247:DNA