Characterization of a ubiquitin-fusion gene from the tobacco hawkmoth, Manduca sexta.
A gene encoding a ubiquitin-fusion protein was isolated from a cDNA library made from the intersegmental muscles (ISM) of the moth, Manduca sexta. The predicted amino acid sequence of this fusion protein is highly conserved when compared to the sequence of homologous proteins from diverse species. The Manduca clone encoding this ubiquitin fusion gene hybridized with a single, abundantly expressed transcript in all tissues examined. In the ISM, the transcript was present at high levels, independent of the developmental stage or hormonal treatment of these muscles. Data from other species indicate that ubiquitin-fusion genes participate in ribosome biogenesis.
Department of Zoology, Morrill Science Center, University of Massachusetts, Amherst 01003.
S T
ST
Bishoff
L M
LM
Schwartz
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D014452:Ubiquitins / Q000096:biosynthesis / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D014452:Ubiquitins; D012313:RNA; D004247:DNA