Comparative metabolic profiling of chloramphenicol by isolated hepatocytes from rat and trout (Oncorhynchus mykiss).
1. Isolated liver cells from rat and rainbow trout were used to study the biotransformation of labelled chloramphenicol (CP). 2. The 3H-CP metabolic rates were 1.7 nmol/hr/10(6) rat hepatocytes and 0.2 nmol/hr/10(6) trout hepatocytes. 3. In rat, as in trout liver cells, the major metabolite was CP-glucuronide. In addition to this conjugate, CP-base, CP-alcohol and CP-oxamic acid were detected in significant amounts. 4. These results are compared with in vivo data on CP metabolism previously obtained in our laboratory in fish and mammals.
Laboratoire des Xénobiotiques, INRA, Toulouse, France.
J P
JP
Cravedi
M
M
Baradat
D000818:Animals; D002701:Chloramphenicol / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D015394:Molecular Structure; D051381:Rats; D011919:Rats, Inbred Strains / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*
D002701:Chloramphenicol