Secretory state of gastric mucosa does not protect against acute gastric mucosal injury induced by hemorrhagic shock in rats.
The protective effect of the secretory state of gastric mucosa against acute gastric mucosal injury due to hemorrhagic shock was investigated in rats with tetragastrin infusion. A total of 26 male Spraque-Dawley rats were divided into 3 groups (n = 6, 8, and 12, respectively). Tetragastrin (8 micrograms/Kg/h) was added into the normal saline infusion (5 ml/h) in group 3, but not in the other 2 groups. The mucosal surface was instilled with 0.1 N HCl (pH = 1) in group 2, with normal saline (pH = 7) in group 1, and with nothing in group 3. Hemorrhagic shock at 40 mmHg of arterial pressure was induced by withdrawing blood from the femoral artery, and was maintained at this level for 20 minutes; later, the shed blood was re-infused. During hemorrhagic shock, scattered areas of white circumscribed white lesions appeared on the mucosal folds of the glandular region in all of group 2 and in 8 (with gastric pH less than 3) of group 3, yet did not appear in group 1 or in the remaining 4 (with gastric pH greater than 3) of group 3. After reinfusion, varying degrees of mucosal hemorrhage from the whitened areas occurred in all of group 2 and in 8 (with pH less than 3) of group 3, but in only one of group 1. The severity of acute gastric mucosal injury in group 2 and group 3 (n = 8, with gastric pH less than 3) was significantly greater than group 1. However, it was not significantly different between groups 2 and 3.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Surgery, Chang Gung Memorial Hospital, Chang Gung Medical College, Tao-Yueng Hsien, Taipei, Taiwan, R.O.C.
C S
CS
Wang
M F
MF
Chen
S Y
SY
Hsu
D000818:Animals; D005745:Gastric Acidity Determination; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012771:Shock, Hemorrhagic / Q000150:complications*; D013276:Stomach Ulcer / Q000517:prevention & control*; D013758:Tetragastrin / Q000494:pharmacology
D013758:Tetragastrin