The effects of anthranilic acid on gene expression.
1. A Drosophila pseudoobscura amylase gene cloned in Escherichia coli is expressed at high levels. The expression of this gene is repressed when glucose (0.5% final concentration) is added to a starch minimal medium culture of E. coli cells containing the amylase plasmid. 2. Addition of anthranilic acid (5 and 7 mM final exogenous concentration) to catabolite repressed cells mimics the action of adenosine 3'5' cyclic monophosphate (cAMP) by depressing the expression of the amylase. 3. The results suggest that anthranilic acid acts either indirectly, possibly through the glucose transport system, or directly, by way of an intercalative model of initiation, to alter the levels of transcription.
Department of Biology, Bucknell University, Lewisburg, PA 17837.
S M
SM
Stevens
C
C
Bosio
J
J
Moehring
E L
EL
Kline
M I
MI
Chernin
D000681:Amylases / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D000242:Cyclic AMP / Q000494:pharmacology; D004330:Drosophila / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005947:Glucose / Q000502:physiology*; D010957:Plasmids; D012329:RNA, Bacterial / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D012329:RNA, Bacterial; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D000242:Cyclic AMP; D000681:Amylases; D005947:Glucose