Multifactorial control of insulin receptor gene expression in human cell lines.
Taking advantage of a computer program to align DNA sequences we scanned the 5'-flanking region of the insulin receptor gene to localize putative regulatory elements. Using this approach as a start point, we investigated the effect of several agents on insulin receptor gene expression in human cells. We found that (i) forskolin reduces insulin receptor mRNA levels; (ii) the effects of either forskolin or dexamethasone on insulin receptor mRNA levels are near completely abolished in glucose-starved cells; (iii) 2-mercaptoethanol and heat shock, besides previously described stress conditions (glycosylatyon inhibitors), reduce insulin receptor mRNA levels.
Briata
P
P
Immunobiology Laboratory, National Cancer Institute (I.S.T.), Genova, Italy.
Gherzi
R
R
D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology; D004247:DNA / Q000032:analysis*; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D008623:Mercaptoethanol / Q000494:pharmacology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012313:RNA / Q000032:analysis; D011972:Receptor, Insulin / Q000187:drug effects / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D005576:Colforsin; D008623:Mercaptoethanol; D012313:RNA; D003907:Dexamethasone; D004247:DNA; D000242:Cyclic AMP; D011972:Receptor, Insulin