Monoclonal antibody (SBU-1 and SBU-3) identification of cells dissociated from the sheep placentomal trophoblast.
We studied the localization of alpha-keratin in the sheep placenta using an alpha-keratin-specific monoclonal antibody (MAb) SBU-1, and examined the feasibility of using this MAb as a marker for determining the purity of isolated uninucleate cells from the placentomal trophoblast. At about 30-50 days of gestation the placentomal and interplacentomal uninucleate cells and some binucleate cells were stained by SBU-1, whereas only the apical region of the syncytial cytoplasm was stained with this MAb. Other cells stained included the uterine and endometrial glandular epithelial cells and fibroblast-like cells in the endometrium and chorionic villi. At about 100-130 days of gestation only the trophoblast uninucleate cells were stained by SBU-1. Approximately 60% of cells isolated from placentomes at 100-130 days of gestation were stained by SBU-1, and they had similar morphological features to the trophoblast uninucleate cells. The number of binucleate cells present was confirmed by their affinity for MAb SBU-3. These results show that MAb SBU-1 is an excellent marker for trophoblast uninucleate cells from placenta of sheep at the later stages of pregnancy.
Lee
C S
CS
Department of Veterinary Preclinical Sciences, University of Melbourne, Parkville, Victoria, Australia.
Ralph
M M
MM
Gogolin-Ewens
K J
KJ
Brandon
M R
MR
D000818:Animals; D000911:Antibodies, Monoclonal; D005347:Fibroblasts / Q000378:metabolism; D005865:Gestational Age; D015726:Giant Cells / Q000378:metabolism; D007633:Keratins / Q000378:metabolism; D010920:Placenta / Q000166:cytology*; D012756:Sheep; D014327:Trophoblasts / Q000166:cytology*
D000911:Antibodies, Monoclonal; D007633:Keratins