Histamine secretion induced by neuromedin-N.
Neuromedin-N dose-dependently stimulated the release of histamine from rat serosal mast cells and was 10 to 100 times less potent than neurotensin. The threshold concentration was 10(-6) M, and 10(-3) M neuromedin-N released 31% of the total cell histamine content. The histamine release induced by neuromedin-N was temperature-dependent with an optimum around 30-37 degrees C. Skin vascular permeability increased dose-dependently in response to intradermal injections of neuromedin-N and this peptide was 10 to 100 times less potent than neurotensin. Mepyramine inhibited the effect on vascular permeability suggesting that the effect of neuromedin-N was mediated via the release of histamine.
Sydbom
A
A
Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Ware
J
J
Schultz
I
I
Mogard
M H
MH
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D009496:Neurotensin / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D011738:Pyrilamine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012711:Serum Albumin, Radio-Iodinated
D010446:Peptide Fragments; D012711:Serum Albumin, Radio-Iodinated; C042090:neuromedin N; D009496:Neurotensin; D011738:Pyrilamine