A molecular genetic linkage map of mouse chromosome 19, including the lpr, Ly-44, and Tdt genes.
The mouse lpr gene, which is an autosomal recessive gene causing autoimmune disease with features of human systemic lupus erythematosus and eventually death from severe immune-complex glomerulonephritis, has been mapped on chromosome 19. To determine its exact chromosomal location, a three-point backcross was carried out by mating (MRL/MpJ-lpr/lpr x MOL-MIT)F1 x MRL/MpJ-lpr/lpr using the genes Ly-44 (lymphocyte differentiation antigen-44) and Tdt (terminal deoxynucleotidyl transferase) as markers. The following order of genes is proposed, with the distances between genes given in parentheses: centromere-Ly-44 (19.3 cM)-lpr (6.1 cM)-Tdt-telomere. The Ly-44a and Tdta alleles are found in all laboratory strains and in the wild Western European subspecies, domesticus and brevirostris. In contrast, the Ly-44b and Tdtb alleles are found in some Asian subspecies, Chinese mice of wild origin, yamashinai and molossinus. Furthermore the third Tdt allele, Tdtc, is detected in castaneus.
Department of Biochemistry, Aichi Prefecture Colony, Japan.
T
T
Watanabe
Y
Y
Sakai
S
S
Miyawaki
A
A
Shimizu
O
O
Koiwai
K
K
Ohno
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics*; D000950:Antigens, Ly / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes; D003433:Crosses, Genetic; D004253:DNA Nucleotidylexotransferase / Q000235:genetics*; D015342:DNA Probes; D005808:Genes, Recessive; D008040:Genetic Linkage; D008232:Lymphoproliferative Disorders / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D000944:Antigens, Differentiation, B-Lymphocyte; D000950:Antigens, Ly; D015342:DNA Probes; C057962:LY 44 antigen; D004253:DNA Nucleotidylexotransferase