Isolation of an early T-cell precursor (CFU-TL) from human bone marrow.
CD2-CD3-CD4-CD8- human bone marrow (BM) cells were previously shown to generate T-cell clones in vitro. This capacity was abolished after treatment of this population with anti-CD7 monoclonal antibody and complement. In this study, using rosetting with sheep erythrocytes, complement-dependent cytotoxicity, and specific immunoadherence method, we isolated a minor BM subset that contained more than 80% CD7+CD2-CD3-CD4-CD8- cells with small lymphoid cell morphology. They comprised most early T-cell precursors (CFU-TL) as they displayed high capacity to generate T-cell clones when cultured in limiting dilutions. CFU-TL nature of these cells was also confirmed by the sequential expression of mature T-cell specific markers on their surface after in vitro induction. This BM subset also contained 2% to 3% CFU-GM precursors. Together, these results pointed to the existence of BM CD7+CD2- precursors with high differentiation potential and showed the commitment of most of them to T-cell lineage.
Bertho
J M
JM
Laboratoire d'Immunologie Cellulaire, CNRS URA 186, hôpital Pitié-Salpètrière, Paris, France.
Mossalayi
M D
MD
Dalloul
A H
AH
Mouterde
G
G
Debre
P
P
D015703:Antigens, CD / Q000032:analysis; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001854:Bone Marrow Cells; D018801:CD2 Antigens; D002454:Cell Differentiation; D002455:Cell Division; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D011971:Receptors, Immunologic / Q000032:analysis; D013601:T-Lymphocytes / Q000166:cytology*; D013997:Time Factors
D015703:Antigens, CD; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D011971:Receptors, Immunologic