Aromatic L-amino-acid decarboxylase (DOPA decarboxylase) gene expression in dopaminergic and serotoninergic cells of the rat brainstem.
In situ hybridization was performed in the rat brain to detect aromatic L-amino acid decarboxylase (AADC) mRNA using 35S-labeled oligonucleotide probes derived from rat kidney AADC cDNA. Results demonstrated AADC mRNA in areas containing dopaminergic and serotoninergic cell bodies. Combined immunohistochemistry for tyrosine- or tryptophan hydroxylase and in situ hybridization for AADC mRNA demonstrated the dopaminergic or serotoninergic nature of cells containing AADC mRNA. Tyrosine hydroxylase-positive mesencephalic neurons containing a very low or no AADC mRNA signal were also observed.
URA CNRS 1200 Laboratoire d'Histologie-Embryologie, Université de Bordeaux II, France.
F
F
Tison
E
E
Normand
M
M
Jaber
I
I
Aubert
B
B
Bloch
D006916:5-Hydroxytryptophan / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000235:genetics* / Q000378:metabolism; D001933:Brain Stem / Q000378:metabolism*; D015870:Gene Expression; D007980:Levodopa / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014365:Tryptophan Hydroxylase / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D012333:RNA, Messenger; D007980:Levodopa; D006916:5-Hydroxytryptophan; D014446:Tyrosine 3-Monooxygenase; D014365:Tryptophan Hydroxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases