Bioneutralization capacity of the antibodies generated in women by the beta subunit of human chorionic gonadotropin (beta hCG) and beta hCG associated with the alpha subunit of ovine luteinizing hormone linked to carriers.
Data is presented on the bioneutralization capacity per unit immunoactivity of 30 serum samples of women immunized with the beta-subunit of human chorionic gonadotropin (beta-hCG) or beta hCG associated noncovalently with the alpha-subunit of ovine luteinizing hormone (alpha-oLH), to form a heterospecies dimer (HSD), which were linked to carriers. The bioassays utilized were inhibition of radioiodinated hCG binding to rat testicular receptors in vitro and the inhibition of hCG induced testosterone production in mice. In both assays, antisera of women immunized with the HSD had a bioactivity to immunoactivity ratio that was about 25% higher, on an average, than antisera of women immunized with beta-hCG, suggesting a better bioneutralization capacity of sera raised by the HSD in women.
Immuno-Endocrinology Group, National Institute of Immunology, Shaheed Jeet Singh Marg, New Delhi, India.
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Pal
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Singh
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Rao
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Talwar
D000328:Adult; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D006063:Chorionic Gonadotropin / Q000008:administration & dosage / Q000276:immunology*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D003269:Contraception, Immunologic; D005260:Female; D015292:Glycoprotein Hormones, alpha Subunit / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007114:Immunization; D000922:Immunotoxins / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000008:administration & dosage / Q000276:immunology*; D011967:Receptors, Gonadotropin / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
D000906:Antibodies; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D015292:Glycoprotein Hormones, alpha Subunit; D000922:Immunotoxins; D010446:Peptide Fragments; D011967:Receptors, Gonadotropin; D013739:Testosterone