Occurrence of pap-, sfa-, and afa-related sequences among F165-positive Escherichia coli from diseased animals.
A total of 160 Escherichia coli positive for F165 fimbrial antigen and isolated from diarrheic and septicemic animals, were examined for the presence of the pap, afa, and sfa/foc operons or related nucleotide sequences using colony hybridization. Most isolates shared DNA sequences with the pap operon sequences alone or in association with afa or sfa. Thus, our results indicate that F165-positive E. coli from diseased animals share DNA sequences with operons coding for adhesins important in human extra-intestinal disease and that multiple adhesin systems are often found in single isolates. However, 20% of the F165-positive isolates did not show any homology with the probes representing the three adhesin systems, suggesting that one of the operons responsible for F165 production could be different from the pap, sfa/foc, and afa operons.
Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Quebec, Canada.
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Harel
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Daigle
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Maiti
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Désautels
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Labigne
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Fairbrother
D018830:Adhesins, Escherichia coli; D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001422:Bacterial Adhesion / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D015342:DNA Probes / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000276:immunology; D005455:Fluorescent Antibody Technique; D009693:Nucleic Acid Hybridization; D009876:Operon; D018805:Sepsis / Q000662:veterinary; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D015342:DNA Probes