Hydroxylation of collagen by lungs of rats administered bleomycin.
Collagen synthesized by tissue minces from lungs of rats administered 1 unit of bleomycin by intratracheal instillation 1 or 2 wk earlier contained relatively more hydroxylysine than did collagen made by lungs from saline-instilled control animals. Most, if not all, of the relative increase in lysine hydroxylation could be localized to the alpha 1 (I) chain of type I collagen. Lung homogenates from bleomycin-treated rats showed increased activity of lysyl hydroxylase (EC 1.14.11.4), the enzyme catalyzing the conversion of collagen-bound lysine to hydroxylysine. Thus, the increased hydroxylation of lysine and of lysine-derived cross-links previously observed in collagen of diseased human lungs and in animal models of lung fibrosis is reflected in an in vitro system.
Department of Internal Medicine, School of Medicine, University of California, Davis.
J A
JA
Last
J E
JE
Gerriets
L C
LC
Armstrong
T R
TR
Gelzleichter
K M
KM
Reiser
D000818:Animals; D001761:Bleomycin; D003094:Collagen / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006900:Hydroxylation; D006901:Hydroxylysine / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D008914:Minoxidil / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000378:metabolism*; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats
D001761:Bleomycin; D006901:Hydroxylysine; D008914:Minoxidil; D003094:Collagen; D006899:Mixed Function Oxygenases; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase