Enhancement of IgG production elicited in mice by treatment with anti-CD8 antibody.
Injection of an anti-CD8 monoclonal antibody into mice was followed by an increase in the production of immunoglobulins of the IgG1, IgG2a and IgG2b, but neither the IgG3 nor the IgM isotypes. A maximal effect was observed 6 days after administration of 100 micrograms antibody. However, no modification of spleen cell proliferation was elicited by the anti-CD8 treatment. Virally induced IgG2a and IgG2b secretion remained unchanged in mice infected with lactate dehydrogenase-elevating virus that received a concomitant injection of anti-CD8 antibody, whereas an enhancement of IgG1 was observed. Simultaneous treatment with an anti-CD4 antibody abrogated the immunoglobulin secretion triggered by anti-CD8. These results suggest that immunoglobulin production in unmanipulated mice is controlled by CD8+ lymphocytes.
Unit of Experimental Medicine, Université Catholique de Louvain, Brussels, Belgium.
J P
JP
Coutelier
D000818:Animals; D000917:Antibody Formation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D066298:In Vitro Techniques; D007518:Isoantibodies / Q000276:immunology; D008212:Lymphocyte Depletion; D051379:Mice; D008808:Mice, Inbred CBA; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D016827:CD8 Antigens; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007518:Isoantibodies