Immunohistochemical detection of the epidermal growth factor receptor in paraffin-embedded human tissues.
Expression of the epidermal growth factor (EGF) receptor was evaluated by immunohistochemical staining of formalin-fixed, paraffin-embedded tumour tissues employing two antibodies raised to short synthetic peptides from the cytoplasmic domain of the molecule. Both antibodies gave concordant staining of a series of bladder cancers known to express or lack EGF receptors. There was no cross-reaction with the related c-erbB-2 protein, which was also over-expressed in some cases. Cancers with EGF receptor expression also expressed high levels of TGF-alpha, a receptor agonist.
ICRF Oncology Group, Hammersmith Hospital, London, U.K.
W J
WJ
Gullick
C M
CM
Hughes
K
K
Mellon
D E
DE
Neal
N R
NR
Lemoine
D000911:Antibodies, Monoclonal; D066246:ErbB Receptors / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D010232:Paraffin; D011518:Proto-Oncogene Proteins / Q000032:analysis; D011519:Proto-Oncogenes; D018719:Erb-b2 Receptor Tyrosine Kinases; D016211:Transforming Growth Factor alpha / Q000032:analysis / Q000235:genetics / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000737:chemistry* / Q000235:genetics
D000911:Antibodies, Monoclonal; D066246:ErbB Receptors; D010232:Paraffin; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D016211:Transforming Growth Factor alpha