[The role of interferons in neuroblastoma. 1: Antiproliferative effects].
Antiproliferative effects of interferon alpha, beta and gamma were investigated on several human neuroblastoma cell lines using the soft agar colony forming assay and the MTT-test. Investigations were carried out in order to prove whether there is any relationship between antiproliferative effects, inhibition of N-myc expression and the 2-5A system. Growth of neuroblastoma cells was inhibited by all three kinds of interferons in a concentration-dependent manner, however, rather high concentrations were necessary in some cell lines. Expression of N-myc oncogen was not inhibited by interferon-beta and no relationship between antiproliferative effects and the 2-5A system was observed. A vector containing a small N-myc fragment in antisense direction was constructed and transferred into the interferon insensitive human neuroblastoma cell line LS. After transformation, LS cells became sensitive to interferon beta: Proliferation as well as N-myc expression were inhibited and these processes are most probably associated with activation of the 2-5A system.
Univ. Kinderklinik Tübingen, Abt. Hämatologie und Onkologie.
G
G
Bruchelt
R
R
Handgretinger
K
K
Schilbach-Stückle
F H
FH
Schilling
P
P
Pollwein
M
M
Schwab
J
J
Treuner
D
D
Niethammer
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005784:Gene Amplification / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D009447:Neuroblastoma / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects*; D014410:Tumor Stem Cell Assay
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase
Die Rolle der Interferone beim Neuroblastom. Teil 1: Antiproliferative Effekte.