Immunohistological identification of interleukin-1 activated chondrocytes.
Interleukin-1 activated chondrocytes have been identified in pig articular cartilage by immunolocalisation using a polyclonal antiserum recognising cytokine induced surface epitopes. Although chondrocytes with membrane staining were noted in all zones of the cartilage, only a proportion of the cells were positive within the range of antiserum dilution used (1:100-1:2000). Maximum staining was found after four days' treatment of cultured cartilage with pig IL-1 alpha, though weak staining was just detectable after treatment for one day at high antiserum concentration. Immunoreactivity was also shown in cartilage which had been cultured with human recombinant IL-1 alpha.
Dingle
J T
JT
Tissue Physiology Department, Strangeways Research Laboratory, Cambridge.
Davies
M E
ME
Mativi
B Y
BY
Middleton
H F
HF
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D046508:Culture Techniques; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006025:Glycosaminoglycans / Q000378:metabolism; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000276:immunology*; D013552:Swine
D000939:Epitopes; D006025:Glycosaminoglycans; D007375:Interleukin-1