[Hybridase cleavage of RNA. I. Oligonucleotide probes for regiospecific RNA cleavage].
New oligonucleotide probes for regiospecific cleavage of RNA molecules by hybridase (RNase H) are suggested. RNase H from E. coli is shown to site-specifically split eight phosphodiester bonds in RNA in the heteroduplex, formed by 5S rRNA and d(ACCACCGCGCT). The partial substitution of deoxycytidines in position 5, 6, 8, 10 of the probe by 2'-O-methylcytidines leads to unique (regiospecific) RNA cleavage between U25 and C26.
V G
VG
Metelev
N F
NF
Krynetskaia
A A
AA
Purmal'
Z A
ZA
Shabarova
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004722:Endoribonucleases / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism; D015345:Oligonucleotide Probes; D012313:RNA / Q000378:metabolism*; D016914:Ribonuclease H
D009692:Nucleic Acid Heteroduplexes; D015345:Oligonucleotide Probes; D012313:RNA; D004722:Endoribonucleases; D016914:Ribonuclease H
Gibridaznoe rasshcheplenie RNK. I. Oligonukleotidnye zondy dlia regiospetsificheskogo rasshchepleniia RNK.