Substance P-like immunoreactivity at the frog neuromuscular junction.
It has been recently shown that frog motoneurons and their nerve terminals contain calcitonin gene-related peptide-like immunoreactivity in large dense-core vesicles (Matteoli et al., Proc. natn. Acad. Sci. U.S.A. 85, 7366-7370, 1988). We report here by an immunofluorescence approach that the same neurons and nerve terminals also contain substance P-like immunoreactivity. The demonstration of substance P-like immunoreactivity in the frog motor nerve endings supports previous data suggesting a physiological role for this peptide in the modulation of cholinergic transmission.
CNR Istituto Fisiologia Centri Nervosi, Milano, Italy.
M
M
Matteoli
C
C
Haimann
P
P
De Camilli
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D009046:Motor Neurons / Q000378:metabolism; D009411:Nerve Endings / Q000378:metabolism; D009469:Neuromuscular Junction / Q000378:metabolism*; D011894:Rana pipiens; D011978:Receptors, Nicotinic / Q000187:drug effects; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism; D013373:Substance P / Q000276:immunology / Q000378:metabolism*
D011978:Receptors, Nicotinic; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide; D000109:Acetylcholine