Direct measurement of the concentration- and time-dependent open probability of the nicotinic acetylcholine receptor channel.
Using the outside-out patch clamp recording technique together with a rapid solution exchange system, we measured ionic currents through nicotinic acetylcholine (ACh) receptor channels from BC3H-1 cells in response to rapid applications of 0.3-1,000 microM ACh. We used nonstationary fluctuation analysis of ensembles of responses to deduce the number of channels in the patch, the maximum open channel probability as a function of ACh concentration and the time course of a fast desensitization process. We found that: (a) Excised patches from BC3H-1 cells typically contain between 50 and 150 functional ACh receptor ion channels. (b) The open channel probability is proportional to [ACh]1.95 at low concentrations of ACh, is half-maximal at 20 microM ACh and saturates above 100 microM ACh. (c) ACh is a very efficacious agonist; 100 microM ACh opens at least 90% of the available channels. This estimate of efficacy is model-independent. (d) The rate of decay of the agonist-induced current is concentration-dependent. In the presence of 100 microM ACh the current decays with a time constant of 50-100 ms. It decays more slowly in the presence of lower concentrations of agonist but is relatively insensitive to voltage.
Department of Anesthesiology, State University of New York, Stony Brook 11794-8480.
J P
JP
Dilger
R S
RS
Brett
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D004553:Electric Conductivity; D007473:Ion Channels / Q000502:physiology*; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D008954:Models, Biological; D011336:Probability; D011978:Receptors, Nicotinic / Q000502:physiology*; D013997:Time Factors
D007473:Ion Channels; D011978:Receptors, Nicotinic