Expression and ontogeny of CD2 on murine B cells.
Expression of CD2 on developing and mature murine B cells was examined by using an antipeptide antiserum (L50). Most Ig-bearing splenic B cells were found to express CD2. Anti-CD5 and anti-B220 mAb divided the peritoneal B cells into two populations expressing high and low levels of these proteins; both populations were found to express uniform levels of CD2. Abelson murine leukemia virus-transformed pre-B cell lines derived from fetal liver and adult bone marrow were analyzed to delineate the ontogeny of CD2 in the B cell lineage. The results show that onset of CD2 expression correlates with the presence of cytoplasmic mu-chain. Therefore, the earliest CD2+ pre-B cell in the developing B cell population appears to be the classical pre-B cell.
Sen
J
J
Division of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA 02115.
Rosenberg
N
N
Burakoff
S J
SJ
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D018801:CD2 Antigens; D018956:CD5 Antigens; D002454:Cell Differentiation; D002472:Cell Transformation, Viral; D005434:Flow Cytometry; D015322:Gene Rearrangement, B-Lymphocyte; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D010529:Peritoneal Cavity / Q000166:cytology; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D011971:Receptors, Immunologic / Q000276:immunology*; D013154:Spleen / Q000166:cytology
D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D018956:CD5 Antigens; D011947:Receptors, Antigen, B-Cell; D011971:Receptors, Immunologic