Assessment by fluorescence-activated cell sorting of whether sperm-associated immunoglobulin (Ig)G and IgA occur on the same sperm population.
Two-color fluorescence-activated cell sorting of antisperm antibody-positive sperm was used to detect simultaneously the presence of immunoglobulin (Ig)A and IgG antisperm antibodies associated in vivo on a man's sperm. Sperm positive for sperm-associated Ig were analyzed using phycoerythrin-conjugated antihuman IgA and fluorescein isothiocyanate-conjugated antihuman IgG; up to 87% of the same spermatozoa were stained with both labels. Sperm positive for only one of the antisperm antibody isotypes stained up to 90% of a man's sperm with only one fluorochrome. Immunocytochemistry studies revealed similar patterns of sperm binding for sperm-associated IgG and IgA. These results suggest that the sperm antigenic determinants reacting with antisperm IgA and IgG are present on the same sperm population at similar locations on the sperm surface.
Haas
G G
GG
University of Oklahoma Health Sciences Center, Oklahoma City.
D'Cruz
O J
OJ
DeBault
L E
LE
D002469:Cell Separation; D000939:Epitopes / Q000032:analysis; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007248:Infertility, Male / Q000276:immunology; D007457:Iodine Radioisotopes; D008297:Male; D010799:Phycoerythrin; D013094:Spermatozoa / Q000276:immunology*; D013861:Thiocyanates
D000939:Epitopes; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D013861:Thiocyanates; D010799:Phycoerythrin; D016650:Fluorescein-5-isothiocyanate