Effects of major histocompatibility genes and antigen delivery on induction of protective mucosal immunity to E. acervulina following immunization with a recombinant merozoite antigen.
Intramuscular immunization with the recombinant p250 surface antigen of Eimeria acervulina merozoite (rEAMZp250) or oral inoculation with live recombinant Escherichia coli expressing the rEAMZp250 protein resulted in antigen-specific T-cell and humoral responses and conferred a significant reduction in mucosal parasitism compared to immunization with the negative control antigen preparation. Among the major histocompatibility complex (MHC) (B)-congenic chickens receiving intramuscular immunization, strain .6-2 (B2-B2) showed significant (P less than 0.05) protection to live E. acervulina challenge compared to the other strains examined. In contrast, strains .C-12(B12B12) and .P-13 (B13B13) showed significant protection among the groups given live recombinant E. coli. In general, strains showing enhanced T-cell responses to the rEAMZp250 protein were better protected compared to those showing minimal or no T-cell responses. Thus the results suggest that the B haplotypes of the host and the mode of antigen presentation influence the outcome of protection following an immunization of chickens with recombinant coccidial antigen.
Protozoan Diseases Laboratory, U.S. Department of Agriculture, BARC-East, Beltsville, Maryland 20705.
H S
HS
Lillehoj
M C
MC
Jenkins
L D
LD
Bacon
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D000953:Antigens, Protozoan / Q000008:administration & dosage*; D003048:Coccidiosis / Q000276:immunology* / Q000517:prevention & control; D004539:Eimeria / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005796:Genes / Q000276:immunology; D007700:Kinetics; D008285:Major Histocompatibility Complex / Q000235:genetics*; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000939:Epitopes; D011993:Recombinant Fusion Proteins