Immune release of histamine and other lipid mediators from guinea-pig isolated kidney: antagonism by BN-52021.
The ability of a specific PAF-receptor antagonist, BN-52021, to control the release of mediators of anaphylaxis from actively sensitized (ovalbumin) guinea-pig has been investigated "in vitro". BN-52021 perfused through the kidney at different molar concentrations (1 x 10(-4)-1 x 10(-5)-1 x 10(-6] prior to antigen challenge neither modified the basal values of perfusion pressure nor stimulated mediator release from the organ. On the contrary, the compound antagonized in a concentration dependent way both vasoconstriction of the renal vessels and the increase in the perfusate of histamine, TXB2 and SRS-A due to antigenic shock. The antagonistic activity of BN-52021 was very consistent at 1 x 10(-4) M at which concentration the immunological release of histamine and TXB2 was reduced by 75%. The beneficial effect of BN-52021 in experimental anaphylaxis of the kidney may have some therapeutic implications principally in those pathological conditions where an abnormal increase of renal histamine and other mediators may compromise the haemodynamic function of this organ.
Department of Pharmacology Chemotherapy and Medical Toxicology, University of Milan, Italy.
F
F
Berti
G
G
Rossoni
P
P
Braquet
D000818:Animals; D001681:Biological Assay; D004224:Diterpenes; D046934:Ginkgolides; D006168:Guinea Pigs; D006636:Histamine Release / Q000187:drug effects* / Q000276:immunology; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects* / Q000276:immunology; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D011863:Radioimmunoassay; D013189:SRS-A / Q000378:metabolism*; D013929:Thromboxane B2 / Q000378:metabolism*
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; D013189:SRS-A; D013929:Thromboxane B2; D010047:Ovalbumin; C045856:ginkgolide B