A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.
Reactivity patterns of seven mouse monoclonal antibodies to human keratin 7 were compared using immunoblotting and immunohistochemistry on cultured cells and normal human and animal tissues. Differences in keratin specificities as determined by two-dimensional immunoblots and interspecies cross-reactivity data on 8 mammalian species suggest that at least six nonidentical epitopes of the keratin 7 molecule are recognized by this panel of reagents. Immunohistochemical examination of a panel of various human neoplasms with monoclonal antibodies monospecific for keratins 7, 18 and 19 revealed potential value of keratin subtyping in differential diagnosis of tumors in general and in subclassification of carcinomas in particular.
Department of Immunology and Molecular Biology, Research Institute of Clinical and Experimental Oncology, Brno., Czechoslovakia.
B
B
Vojtĕsek
Z
Z
Stasková
R
R
Nenutil
J
J
Bártková
J
J
Kovarík
A
A
Rejthar
J
J
Bártek
D000818:Animals; D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000175:diagnosis; D003110:Colonic Neoplasms / Q000175:diagnosis; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D007381:Intermediate Filament Proteins / Q000032:analysis; D007633:Keratins / Q000032:analysis / Q000276:immunology*; D009369:Neoplasms / Q000032:analysis / Q000175:diagnosis*; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis
D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D007633:Keratins