[Prenatal diagnosis of hemophilia A by DNA analysis].
Hemophilia A caused by defect in the gene of factor VIII is an inherited bleeding disorder most commonly seen in man. Prenatal diagnosis and carrier detection of hemophilia have been done through RFLP analysis in recent years. By use of Bc1I/FVIII-i18, XbaI/FVIII-i22, Bc1I/St14, TaqI/St14 and Bg1II/DX 13 RFLP analysis, prenatal diagnosis of 16 cases at risk of hemophilia A were performed. The results of gene diagnosis showed that two out of 16 pregnant women were normal and therefore prenatal diagnosis was not needed. Among the 14 fetuses at risk four normals and 4 carriers were found in the 8 female fetuses, while in the 6 male fetuses 3 were identified as normal and 3 affected.
Wu
G
G
Institute of Basic Medical Sciences, Beijing.
D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis*; D006467:Hemophilia A / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008297:Male; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*