In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
The protein kinase MAP kinase, also called MAP2 kinase, is a serine/threonine kinase whose activation and phosphorylation are induced by a variety of mitogens, and which is thought to have a critical role in a network of protein kinases in mitogenic signal transduction. A burst in kinase activation and protein phosphorylation may also be important in triggering the dramatic reorganization of the cell during the transition from interphase to mitosis. The interphase-metaphase transition of microtubule arrays is under the control of p34cdc2 kinase, a central control element in the G2-M transition of the cell cycle. Here we show that a Xenopus kinase, closely related to the mitogen-activated mammalian MAP kinase, is phosphorylated and activated during M phase of meiotic and mitotic cell cycles, and that the interphase-metaphase transition of microtubule arrays can be induced by the addition of purified Xenopus M phase-activated MAP kinase or mammalian mitogen-activated MAP kinase to interphase extracts in vitro.
Gotoh
Y
Y
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Nishida
E
E
Matsuda
S
S
Shiina
N
N
Kosako
H
H
Shiokawa
K
K
Akiyama
T
T
Ohta
K
K
Sakai
H
H
D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D004789:Enzyme Activation; D007399:Interphase / Q000502:physiology; D008540:Meiosis / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis / Q000502:physiology*; D008970:Molecular Weight; D004676:Myelin Basic Protein / Q000378:metabolism; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D010766:Phosphorylation; D011374:Progesterone / Q000494:pharmacology; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D014981:Xenopus / Q000378:metabolism*
D008869:Microtubule-Associated Proteins; D004676:Myelin Basic Protein; D011374:Progesterone; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases