HLA class I homologous transcripts in the human embryonal carcinoma cell line Tera-2.
We have used the human teratocarcinoma-derived embryonal carcinoma cell line Tera-2 cl. 13 to explore the putative expression of novel HLA class I(-like) genes. Serological analyses revealed that Tera-2 cells do not express polymorphic HLA class I (-A, -B, -C) specificities, but do express HLA class I-like antigens. These phenotypic properties parallel those of certain mouse embryonal carcinoma cells. To study the expression of HLA class I(-like) genes in the Tera-2 cells two different approaches were used. Screening of a Tera-2 cDNA library with a full-length HLA class I cDNA probe under conditions that would allow for the identification of relatively distinct HLA class I-like sequences yielded 27 positive clones, all of which were of the regular HLA-A, -B, -C type. Reverse northern hybridizations of the restriction enzyme-digested Tlab region comprising cosmids with Tera-2 cDNA as the probe resulted in the identification of several putative human genes whose equivalents map within the mouse Tla region. However, none of these genes appeared to be structurally related to HLA class I. A putative H3.3 histone gene was identified in the proximal Tla region of the C57BL/10 mouse. It is concluded that no structural homologues of mouse Qa/Tla genes are expressed in the human developmental cell line Tera-2.
Rinke de Wit
T F
TF
Department of Immunohaematology and Bloodbank, Leiden University Hospital, The Netherlands.
Struyk
L
L
Vloemans
S
S
Glazebrook
J
J
Boyle
J M
JM
Stern
P L
PL
van den Elsen
P J
PJ
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003360:Cosmids; D015870:Gene Expression; D015723:Gene Library; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006657:Histones / Q000235:genetics; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis; D013724:Teratoma / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D015395:Histocompatibility Antigens Class I; D006657:Histones; D015345:Oligonucleotide Probes; D012313:RNA