Human immunodeficiency virus inhibition by lysed leukocyte ultrafiltrate.
The in vitro effect of partially purified lysed leukocyte ultrafiltrate (LLU) on LAV 1 strain of the human immunodeficiency virus (HIV) was investigated. Decrease of virus production as a function of LLU concentration was observed while the phenotype of cultured lymphocytes has remained unchanged. We observed the same depletion of helper-inducer T-cells in samples treated with LLU as well as in infected controls. LLU did not influence significantly the growth of cultured lymphocytes. Following the effect of LLU on RNA-dependent DNA polymerase, inhibition of the LAV 1 reverse transcriptase by LLU was observed. This inhibition was shown to be LLU dose-dependent, considering its content of proteins and orcinol-reactive material. Kinetic studies revealed that inhibition is reversible and competitive with the substrate (3HTTP) but not with template-primer (poly rA-oligo dT).
Institute of Virology, Slovak Academy of Sciences, Bratislava.
H
H
Schmidtmayerová
J C
JC
Chermann
F
F
Rey
F
F
Barré-Sinoussi
V
V
Mayer
D001667:Binding, Competitive; D002478:Cells, Cultured; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D013379:Substrate Specificity; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014462:Ultrafiltration
D012194:RNA-Directed DNA Polymerase