[Changes in cell cycle of human hepatoma xenograft in nude mice after irradiation].
Cell kinetics in human hepatoma xenograft in nude mice after gamma irradiation was studied using flow cytometry (FCM) method and the changes of AFP in the xenograft were measured by radioimmunological assay. After 10 Gy irradiation, a marked tumor growth delay for 10 days was observed. Cell cycle analysis revealed an acute but temporary block of cell cycling at G2. About 58% cells were in the G2 phase lasting for 90 hours post-irradiation. A concomitant decrease in serum AFP determined by RIA was also observed. The results indicate that the human hepatoma was quite radio-sensitive.
Cancer Hospital, Shanghai Medical University.
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Cao
D000818:Animals; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D008113:Liver Neoplasms / Q000097:blood / Q000473:pathology* / Q000532:radiotherapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D000509:alpha-Fetoproteins / Q000032:analysis
D004273:DNA, Neoplasm; D000509:alpha-Fetoproteins