ACh and 5-HT induced changes in the concentration of cytosolic inositol trisphosphate (InsP3) and inositol bisphosphate (InsP2) in the ABRM of Mytilus edulis L.
1. For determination of the phosphoinositides and inositol phosphates present in anterior byssus retractor muscle (ABRM) of Mytilus edulis fiber bundles of this muscle were incubated with [3H]-inositol. Close-to-equilibrium labelling was achieved after 14-17 hr of incubation. 2. The phosphoinositides formed during incubation were identified as phosphatidylinositolphosphates by thin layer chromatography and as glycerophosphoryl esters by anion-exchange chromatography after deacylation. Besides PtdIns, PtdInsP and PtdInsP2 two labelled products are formed, which could not be identified. 3. Inositol phosphates were separated by anion-exchange chromatography. InsP, InsP2 and InsP3 are present, while InsP4 seemed to be absent. 4. Incubation of pre-labelled fibers with ACh induces the accumulation of InsP3 and InsP2 immediately. While 5-Ht accomplishes the accumulation after a lag time of 25 sec. The concentration of cytosolic InsP does not change.
Institut für Tierphysiologie, Freie Universität Berlin, F.R.G.
G
G
Hellwig
R K
RK
Achazi
D000109:Acetylcholine / Q000502:physiology*; D000818:Animals; D049872:Bivalvia / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D003600:Cytosol / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007295:Inositol Phosphates / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D010716:Phosphatidylinositols / Q000032:analysis; D012701:Serotonin / Q000502:physiology*
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; C051854:inositol 3,4-bisphosphate; D012701:Serotonin; D015544:Inositol 1,4,5-Trisphosphate; D000109:Acetylcholine