A study of immunoglobulin-containing and other cells in ovine aural squamous cell carcinoma.
The peroxidase-antiperoxidase method was used to quantify immunoglobulin (Ig)-containing cells at the host-tumour interface in ovine aural squamous cell carcinoma lesions at various stages of development. IgM-containing cells were never observed; only scattered, but strongly staining, IgA-containing cells were present. IgG-containing cells predominated; their prevalence increased from 0.17 +/- 0.34 cells per high power field (hpf; mean +/- standard deviation) in precursor lesions to a maximum of 13.6 +/- 2.6 cells per hpf in overt tumours which had not metastasized. Their prevalence in overt tumours which had metastasized was 4.6 +/- 3.9 cells per hpf. Quantitation of pyroninophils in sections stained by the methyl green-pyronin method showed a greater prevalence of plasma cells than IgG-containing cells, but a similar pattern of change was apparent. Mast cells were most prevalent in early stages, but no significant change in eosinophil numbers was observed.
Graduate School of Tropical Veterinary Science, James Cook University of North Queensland, Townsville, Australia.
W O
WO
Nuttall
P W
PW
Ladds
P W
PW
Daniels
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D004428:Ear Neoplasms / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D005260:Female; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000378:metabolism*; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000378:metabolism* / Q000473:pathology; D013194:Staining and Labeling
D007136:Immunoglobulins