Group IIIA-metal hydroxides indirectly neutralize the voltage sensor of the voltage-dependent mitochondrial channel, VDAC, by interacting with a dynamic binding site.
The voltage-dependent, anion-selective mitochondrial channel, VDAC, undergoes two different conformational changes from the open to a closed state under positive and negative applied electric fields. Micromolar quantities of aluminum hydroxide and other metal trihydroxides have recently been shown to be able to inhibit this voltage-dependent closure (Dill et al. (1987) J. Membr. Biol. 99, 187-196; Zhang and Colombini (1989) Biochim. Biophys. Acta 991, 68-78). It was suggested that the inhibition results from the neutralization of the positively charged voltage sensors by the metal species. In the present study, the dynamics of the metal-binding site accompanying channel closure was investigated by adding In(OH)3 to only one side of the membrane and examining its effect on the channel's gating processes. Indium added to open channels inhibited channel closure only when the metal-containing side was on the lower potential side of the applied field. If indium was added only to the higher-potential side, the channels closed and tended to remain closed after the field was abolished. The addition of metal hydroxide after closing the channels with a negative potential on the metal side did not result in channel re-opening as would be expected for sensor neutralization. Inhibition occurred immediately, however, if the channels were first allowed to open briefly. The closed-state selectivity seemed to be very similar in the absence or presence of the metal, indicating that the metal-binding sites are not located within the pore of the channel in the closed conformation. The results are consistent with a voltage-dependent translocation across the membrane of each of two metal-binding sites on VDAC. This translocation is tightly coupled with channel opening and closing.
Zhang
D W
DW
Department of Zoology, University of Maryland, College Park 20742.
Colombini
M
M
D000838:Anions; D004553:Electric Conductivity; D005656:Fungal Proteins; D005708:Gallium / Q000494:pharmacology; D006878:Hydroxides / Q000494:pharmacology*; D066298:In Vitro Techniques; D007204:Indium / Q000494:pharmacology; D007425:Intracellular Membranes / Q000502:physiology; D007473:Ion Channels / Q000187:drug effects*; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000502:physiology; D008928:Mitochondria / Q000502:physiology*; D009492:Neurospora crassa; D011487:Protein Conformation / Q000187:drug effects
D000838:Anions; D005656:Fungal Proteins; D006878:Hydroxides; D007473:Ion Channels; D008565:Membrane Proteins; D007204:Indium; C020758:indium trichloride; C034163:gallium chloride; D005708:Gallium