B-cell differentiation following autologous, conventional, or T-cell depleted bone marrow transplantation: a recapitulation of normal B-cell ontogeny.
The circulating lymphocytes of 88 consecutive patients following autologous, conventional, or T-cell depleted bone marrow transplantation were serially analyzed for B-cell surface antigen expression and function. In the majority of patients, except for those who developed chronic graft-versus-host disease, the number of circulating CD20+ B cell normalized by the fourth posttransplant month. The earliest detectable B cells normally expressed HLA-DR, CD19, surface immunoglobulin (slg), CD21, Leu-8, and lacked expression of CD10 (CALLA). In addition, the circulating B cells expressed CD1c, CD38, CD5, and CD23 for the first year following transplant, antigens that are normally expressed on a small percentage of circulating B cells in normal adults, but highly expressed on cord blood B cells. Similar to cord blood B cells, patient B cells isolated during the first year following transplant, proliferated normally to Staphylococcus aureus Cowan strain I (SAC), and produced IgM, but minimal or no IgG when stimulated with pokeweed mitogen and SAC, unlike normal adult B cells that produce both. The similar phenotype and function of posttransplant and cord blood B cells, and their similar rate of decline in patients and normal children adds further evidence to support the hypothesis that B-cell differentiation posttransplant is recapitulating normal B-cell ontogeny.
Bone Marrow Transplantation Service, Memorial Sloan Kettering Cancer Center, New York, NY 10021.
T N
TN
Small
C A
CA
Keever
S
S
Weiner-Fedus
G
G
Heller
R J
RJ
O'Reilly
N
N
Flomenberg
D000328:Adult; D000375:Aging; D015703:Antigens, CD / Q000032:analysis; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D002648:Child; D002675:Child, Preschool; D006086:Graft vs Host Disease / Q000097:blood; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D016130:Immunophenotyping; D007231:Infant, Newborn; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D013211:Staphylococcus aureus / Q000276:immunology; D013601:T-Lymphocytes / Q000502:physiology*
D015703:Antigens, CD; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte; D006684:HLA-DR Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M