ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex.
Recruitment of neutrophils to sites of inflammation is mediated in part by endothelial leukocyte adhesion molecule-1 (ELAM-1), which is expressed on activated endothelial cells of the blood vessel walls. ELAM-1 is a member of the LEC-CAM or selectin family of adhesion molecules that contain a lectin motif thought to recognize carbohydrate ligands. In this report, cell adhesion by ELAM-1 is shown to be mediated by a carbohydrate ligand, sialyl-Lewis X (SLex; NeuAc alpha 2,3Gal beta 1,4(Fuc alpha 1,3)-GlcNAc-), a terminal structure found on cell-surface glycoprotein and glycolipid carbohydrate groups of neutrophils.
Cytel Corp., La Jolla, CA 92037.
M L
ML
Phillips
E
E
Nudelman
F C
FC
Gaeta
M
M
Perez
A K
AK
Singhal
S
S
Hakomori
J C
JC
Paulson
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000502:physiology*; D002460:Cell Line; D006224:Cricetinae; D019040:E-Selectin; D006031:Glycosylation; D006801:Humans; D016256:Lewis X Antigen / Q000737:chemistry / Q000502:physiology*; D008024:Ligands; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016256:Lewis X Antigen; D008024:Ligands; D009439:Neuraminidase