Effects of ruthenium red intraperitoneally injected to adult rats, on the uptake and release of neurotransmitters from brain synaptosomes.
Effect of intraperitoneal (i.p.) injection of ruthenium red (RuR) to adults rats on catecholamine (CA) uptake and dopamine (DA) release was evaluated in brain synaptosomal fractions. No effect on CA uptake by synaptosomal fractions was seen when RuR was added in vitro. There was no effect of RuR injection i.p. to rats, on CA uptake by their brain synaptosomal fractions. A blocking effect on depolarizing induced released of DA from brain synaptosomal fraction obtained from rats previously injected i.p. with RuR was seen. A similar effect was observed on the basal DA efflux from synaptosomes loaded with (14C)DA obtained from rats i.p. injected with RuR. It is concluded that RuR, a non-liposoluble substance, when injected i.p. reaches the brain parenchyma through areas devoid of blood-brain barrier and interferes with Ca(++)-dependent release of neurotransmitters, inducing cerebral hyperexcitability and convulsions.
Unidad de Investigación Biomédica de Occidente, IMSS, Universidad de Guadalajara.
A
A
Feria-Velasco
A
A
Morales-Villagrán
G
G
Tapia-Arizmendi
J
J
Arauz-Contreras
A
A
Espinosa de los Monteros
D000818:Animals; D001812:Blood-Brain Barrier; D001923:Brain Chemistry / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D003292:Convulsants / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D007274:Injections, Intraperitoneal; D018377:Neurotransmitter Agents / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012430:Ruthenium Red / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism
D002121:Calcium Channel Blockers; D003292:Convulsants; D018377:Neurotransmitter Agents; D012430:Ruthenium Red; D002118:Calcium