Prevalence of HIV infection in seronegative high-risk individuals examined by virus isolation and PCR.
HIV seronegative individuals with high-risk behavior were tested for HIV infection by sensitive virus isolation techniques using T4 lymphocytes and monocyte/macrophages, and by detection of proviral DNA using PCR with three different sets of nested primers. No evidence of HIV infection was found among the 31 seronegative high-risk subjects, either by virus isolation of by PCR (97.5% confidence limits, 0-11). Our results indicate that ongoing HIV infection in seronegative persons at high risk of infection is a rare event.
Department of Virology, Statens Seruminstitut, Copenhagen, Denmark.
C
C
Nielsen
L S
LS
Teglbjaerg
C
C
Pedersen
J D
JD
Lundgren
C M
CM
Nielsen
B F
BF
Vestergaard
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry; D005260:Female; D006679:HIV Seropositivity / Q000175:diagnosis*; D015528:HIV Seroprevalence; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012307:Risk Factors