Enhancement of human basophil histamine release by interleukin 5.
Substantial evidence indicates a close relationship between eosinophils and basophils. We examined whether interleukin 5 (IL-5), known to be eosinophilopoietic and capable of selectively regulating eosinophil functions, has an affect on basophil functions. IL-5 enhanced basophil histamine release evoked by anti-IgE, formyl-methionyl-leucyl-phenylalanine, or ionophore A23187 at picomolar concentrations. Direct action of IL-5 on human basophils was confirmed using highly purified basophil populations. These observations reveal the novel fact that IL-5 is able to modulate basophil functions as well as eosinophil functions, and suggest that normal human basophils possess functional receptors for IL-5.
Department of Medicine and Physical Therapy, University of Tokyo School of Medicine, Japan.
K
K
Hirai
M
M
Yamaguchi
Y
Y
Misaki
T
T
Takaishi
K
K
Ohta
Y
Y
Morita
K
K
Ito
T
T
Miyamoto
D001491:Basophils / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology
D015848:Interleukin-5; D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006632:Histamine