Three non-repeated transmission blocking epitopes recognized in the 21 kD surface antigen of zygotes-ookinetes of Plasmodium berghei.
Two-site and competitive ELISA have been developed against a surface antigen of zygotes-ookinetes of Plasmodium berghei using monoclonal antibodies (MoAbs) which block transmission of the parasite to the mosquito. Three such MoAbs have been studied, each of which recognized a protein of an Mr 21 kD (Pbs21) using immunoblot techniques. The assays showed that there are at least 3 single B-cell epitopes expressed in Pbs21. One epitope recognized by MoAb 17.9 is conformation dependent and antibodies bound to it interfered with other MoAbs (12.1 and 13.1) each recognizing a different, apparently linear epitope. Glycosylation might not be relevant to the binding of any of the antibodies tested to their respective epitopes.
Tirawanchai
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Department of Pure and Applied Biology, Imperial College of Science, London, UK.
Sinden
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D000954:Antigens, Surface / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology; D001667:Binding, Competitive / Q000276:immunology; D002241:Carbohydrates / Q000276:immunology; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D015151:Immunoblotting; D051379:Mice; D010962:Plasmodium berghei / Q000276:immunology* / Q000378:metabolism; D011499:Protein Processing, Post-Translational
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D002241:Carbohydrates; D000939:Epitopes