Semiautomated autologous bone marrow processing for transplantation.
This paper describes the development of a semiautomated procedure for autologous bone marrow processing, prior to ex vivo manipulation and/or cryopreservation. This procedure was employed with a pediatric bowl (125 ml Latham bowl) and the automated DuPont Stericell processor. We have obtained a mononuclear cell recovery of 85% and a hemopoietic progenitor cell recovery of 81% (CFU-GM; BFU-E), with a red cell removal of 84%. We believe that a reliable and standardized bone marrow processing procedure is the basic necessity for a bone marrow transplantation program.
Pierelli
L
L
Istituto di Semeiotica Medica, Università, Cattolica del Sacro Cuore, Rome, Italy.
Menichella
G
G
Paoloni
A
A
Serafini
R
R
Vittori
M
M
Foddai
M L
ML
Meoni
F
F
Rossi
P L
PL
Leone
G
G
Mango
G
G
D001331:Automation / Q000295:instrumentation; D001853:Bone Marrow / Q000473:pathology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D002452:Cell Count; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D004912:Erythrocytes; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D014182:Transplantation, Autologous