Diagnosis of human schistosomiasis by detection of circulating cathodic antigen with a monoclonal antibody.
Monoclonal antibody (MAb) 5H11 reacted with repeating epitopes on Schistosoma mansoni circulating cathodic antigen (CCA) and detected CCA in sera of Egyptian S. mansoni-infected patients. MAb 5H11 was both capture and biotinylated detection antibody in a sandwich ELISA of trichloroacetic acid-pretreated serum samples. Sera of patients with 7-500 eggs/g of stool were positive by MAb 5H11-CCA sandwich ELISA. Stool egg counts and CCA serum levels correlated (r = .52), and CCA levels decreased by 4 weeks after praziquantel treatment in patients with pretreatment egg counts of greater than or equal to 50/g of stool (P less than .05). Sera of Schistosoma haematobium-infected patients, uninfected individuals, and most patients with other helminths were negative in this assay. The MAb 5H11-CCA sandwich ELISA appears sensitive and specific for immunodetection of active schistosomiasis mansoni and useful for monitoring its chemotherapy.
Barsoum
I S
IS
Department of Microbiology, Vanderbilt University School of Medicine, Nashville, Tennessee.
Kamal
K A
KA
Bassily
S
S
Deelder
A M
AM
Colley
D G
DG
D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000097:blood* / Q000276:immunology; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D006023:Glycoproteins / Q000097:blood*; D015801:Helminth Proteins / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010270:Parasite Egg Count; D011223:Praziquantel / Q000627:therapeutic use; D012550:Schistosoma mansoni / Q000276:immunology* / Q000302:isolation & purification; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000188:drug therapy
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; C091532:CCA protein, Schistosoma mansoni; D006023:Glycoproteins; D015801:Helminth Proteins; D011223:Praziquantel