Genes for Plasmodium falciparum surface antigens cloned by expression in COS cells.
Two genes encoding membrane antigens of Plasmodium falciparum were isolated by transient expression in mammalian cells and selection with human immune sera from African adults exposed to P. falciparum malaria. COS-7 cells were transfected with a plasmid expression library constructed from P. falciparum genomic DNA, and cells expressing reactive malaria antigens on their surface were enriched by adherence to antibody-coated dishes. One of the genes isolated is distinctive in that it does not contain repeat sequences typical of many malarial genes cloned by immunoscreening of bacterial expression libraries. The second gene apparently encodes a polymorphic version of the P. falciparum merozoite surface antigen Ag513, since the two sequences are identical in the 5' and 3' coding regions but diverge completely in the center. The COS-7 expression system provides an alternate means for cloning genes encoding malarial membrane antigens by using those antibodies in complex immune sera that bind membrane-associated, nondenatured molecules.
Elliott
J F
JF
DNAX Research Institute, Palo Alto, CA 94304-1104.
Albrecht
G R
GR
Gilladoga
A
A
Handunnetti
S M
SM
Neequaye
J
J
Lallinger
G
G
Minjas
J N
JN
Howard
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D005796:Genes; D015698:Genomic Library; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D014162:Transfection
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015345:Oligonucleotide Probes; D012313:RNA; D004247:DNA