Glucanase-producing organisms in human dental plaques.
Selective media were used to isolate a wide range of bacteria from sixty human dental plaques. Glucanase activities of the isolates were determined on dextran- and starch-containing media. All sixty samples of dental plaque yielded some colonies showing amylolytic and dextranolytic activities. The glucanase-producing organisms comprised 20% of the isolates. Of these 38% were Gram-positive rods, 27% Gram-positive cocci, 28% Gram-negative rods and 7% were Gram-negative cocci. The cultural groups most commonly represented among the glucanase-producing isolates were Actinomycetaceae, streptococci, haemophili and Gram-negative anaerobes. Species prominent among these isolates included Streptococcus sanguis, Streptococcus mitior, Actinomyces naeslundii, Actinomyces viscosus, Bacterionema matruchotii, Bifidobacterium sp. and Bacteroides sp. No isolates capable of degrading starch or dextran were identified as Streptococcus milleri, Rothia dentocariosa or Fusobacterium sp. This study has shown that a wide range of bacterial species commonly isolated from human dental plaques exhibit both amylolytic and dextranolytic activities. In order to understand glucan metabolism in human dental plaques further investigation of these catabolic activities is necessary.
Department of Preventive and Community Dentistry, University of Melbourne, Victoria, Australia.
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Johnson
D001419:Bacteria / Q000201:enzymology* / Q000302:isolation & purification; D003470:Culture Media; D003773:Dental Plaque / Q000382:microbiology*; D003911:Dextrans / Q000378:metabolism; D005260:Female; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D013213:Starch / Q000378:metabolism
D003470:Culture Media; D003911:Dextrans; D013213:Starch; D006026:Glycoside Hydrolases; C021780:exo-1,3-alpha-glucanase