Inactivation of chemotactic activity of C5a by the serratial 56-kilodalton protease.
The effects of the 56-kilodalton protease (56K protease) from Serratia marcescens on complement-derived chemotactic activity were examined. Fresh human serum was incubated with zymosan to produce C5a. This activated serum was then incubated with various concentrations of 56K protease, and the chemotactic activity of mouse peritoneal exudate polymorphonuclear leukocytes (PMN) and macrophages was evaluated. A significant dose-dependent decrease of chemotactic activity was observed after protease treatment. Furthermore, treatment of human recombinant C5a with 56K protease at a dose of 1.0 microgram/ml resulted in a complete loss of chemotactic activity. When the living bacteria of the virulent strain, which produced about 10 times more protease than did the less virulent strain, were injected intraperitoneally into mice, the magnitude of infiltration of polymorphonuclear leukocytes into the peritoneal cavity was much lower than that caused by the less virulent strain. Because complement-dependent chemotactic activity is an initial response to bacterial infection, these results suggest indirect pathogenic functions of serratial proteases that suppress chemotactic activity.
Department of Microbiology, Kumamoto University Medical School, Japan.
T
T
Oda
Y
Y
Kojima
T
T
Akaike
S
S
Ijiri
A
A
Molla
H
H
Maeda
D001770:Blood Bactericidal Activity; D002630:Chemotactic Factors / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte; D015936:Complement C5a / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000502:physiology; D009504:Neutrophils / Q000502:physiology; D010447:Peptide Hydrolases / Q000378:metabolism*; D012706:Serratia marcescens / Q000201:enzymology*; D000511:alpha-Macroglobulins / Q000378:metabolism
D002630:Chemotactic Factors; D000511:alpha-Macroglobulins; D015936:Complement C5a; D010447:Peptide Hydrolases