Expression of rat prolactin-like protein B in deciduoma of pseudopregnant rat and in decidua during early pregnancy.
The rat PRL-like protein-B (rPLP-B) complementary DNA (cDNA), originally cloned from a late term placental library, hybridizes to transcripts in the deciduoma tissue artificially produced in pseudopregnant rats. The expression of rPLP-B in deciduoma is first observed 48 h (pseudopregnancy day 7) after the deciduogenic stimulus and increases to a maximum by 96-120 h (pseudopregnancy day 9-10). In situ hybridization studies show that rPLP-B hybridizes specifically to the antimesometrial cells of deciduoma tissue in day 9 pseudopregnant rats and to the homologous cells of the decidua that surround the embryo-trophoblastic structure at day 9 of pregnancy. A temporal study on the expression of rPLP-B during pregnancy shows that rPLP-B is concomitantly expressed by the decidual cells of maternal origin and the cytotrophoblast of fetal origin around day 13 of pregnancy.
Croze
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F
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Kennedy
T G
TG
Schroedter
I C
IC
Friesen
H G
HG
D000818:Animals; D015342:DNA Probes; D003656:Decidua / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005333:Fetus / Q000378:metabolism; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010052:Ovariectomy; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood / Q000235:genetics*; D011270:Pregnancy, Animal / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D011555:Pseudopregnancy / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic / Q000187:drug effects
D015342:DNA Probes; D011257:Pregnancy Proteins; D012333:RNA, Messenger; C066551:prolactin-like protein B; D011374:Progesterone; D004958:Estradiol; D012313:RNA