[A method for determining urea by using immobilized urease].
An available method was developed for manual measurements of urea in biological fluids with the use of urease immobilized in sepharose gel. The suggested technique is more reagent-saving (the same reagent may be used for up to 500 times), 3-6-fold more rapid, and twice more sensitive than the routine phenolhypochlorite method with soluble urease. The method is characterized by a high analytical reliability, its results are in high correlation with the soluble urease procedure and with the diacetylmonoxime method (r = 07988 and 0.995, respectively). It is particularly fit for rapid analysis of the urea and convenient for small laboratories.
I P
IP
Ivanov
S I
SI
Danev
Ch D
ChD
Palashev
D G
DG
Dimitrov
D004800:Enzymes, Immobilized; D006801:Humans; D006868:Hydrolysis; D007202:Indicators and Reagents; D007674:Kidney Diseases / Q000175:diagnosis; D019800:Phenol; D010636:Phenols; D015203:Reproducibility of Results; D012685:Sepharose; D014508:Urea / Q000097:blood*; D014510:Urease
D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D010636:Phenols; D019800:Phenol; D014508:Urea; D012685:Sepharose; D014510:Urease
Metod opredeleniia mocheviny pri pomoshchi immobilizovannoĭ ureazy.