Effects of azelastine on neutrophil chemotaxis, phagocytosis and oxygen radical generation.
The effects of azelastine, an orally active anti-allergic drug, on several inflammatory parameters of human neutrophils, including human neutrophil chemotaxis, phagocytosis and generation of reactive oxygen species (ROS), was examined. ROS generated in a cell-free, xanthine-xanthine oxidase system was also assessed. The species investigated were superoxide radical anion (O2-), hydrogen peroxide (H2O2) and hydroxyl radical (OH.). Azelastine significantly inhibited human neutrophil phagocytosis and the generation of O2-, H2O2, OH. by human neutrophils. However, the drug did not markedly affect human neutrophil chemotaxis or the ROS levels generated in the xanthine-xanthine oxidase system. The present study indicates that azelastine may exert an anti-inflammatory action by inhibiting human neutrophil phagocytosis as well as oxygen radical generation at the sites of inflammation.
Department of Dermatology, Kansai Medical University, Osaka, Japan.
H
H
Akamatsu
Y
Y
Miyachi
Y
Y
Asada
Y
Y
Niwa
D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D006878:Hydroxides / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D010793:Phthalazines / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism*
D006634:Histamine H1 Antagonists; D006878:Hydroxides; D010793:Phthalazines; D013481:Superoxides; D006861:Hydrogen Peroxide; C020976:azelastine