L-argininosuccinate modulates L-glutamate response in acutely isolated cerebellar neurons of immature rat.
Fura-2 microfluorometry revealed that as little as 10(-13) M L-argininosuccinate, an intermediate of the L-arginine synthesizing pathway, is sufficient to reduce the increase in intracellular free calcium produced by L-glutamate in the acutely isolated cerebellar neurons of immature rats. In these neurons L-argininosuccinate also reduced the response to quisqualate, but not the response to kainate or N-methyl-D-aspartate. The results suggest that L-argininosuccinate may function as a transmitter or modulator in the brain.
Department of Anatomy, Faculty of Medicine, Kagoshima University, Japan.
H
H
Nakamura
T
T
Yada
T
T
Saheki
T
T
Noda
S
S
Nakagawa
D000818:Animals; D001125:Argininosuccinic Acid / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016257:Fura-2; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D016318:Quisqualic Acid / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence
D005971:Glutamates; D001125:Argininosuccinic Acid; D018698:Glutamic Acid; D016202:N-Methylaspartate; D016318:Quisqualic Acid; D002118:Calcium; D016257:Fura-2