Monoclonal antibody studies suggest a catalytic site at the interface between domains in creatine kinase.
We have located the epitopes recognized by four different monoclonal antibodies which bind to partially unfolded creatine kinase (CK) (ATP: creatine N-phosphotransferase, EC 2.7.3.2) but not to the native enzyme. The epitopes appear to be buried within the CK structure in its native, proteinase-resistant, state. When the epitopes are made accessible to antibody by mild denaturation, CK becomes enzymically-inactive and can be cleaved by proteinase V8 into two large fragments which retain the epitopes and may represent domains. Epitopes on each V8 fragment are associated with highly conserved sequences and are brought physically close to the active site of the enzyme during the later stages of CK refolding and reactivation. The results suggest a catalytic site formed at the interface between two domains which carry the epitopes on their interacting surfaces. Separation of loosely associated domains before or during immunization may account for the origin of antibodies against buried epitopes.
Research Division, N.E. Wales Institute, Deeside, Clwyd, U.K.
G E
GE
Morris
A J
AJ
Cartwright
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002645:Chickens; D003402:Creatine Kinase / Q000276:immunology / Q000378:metabolism*; D003488:Cyanogen Bromide; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007527:Isoenzymes; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D010446:Peptide Fragments / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007527:Isoenzymes; D010446:Peptide Fragments; D003402:Creatine Kinase; D003488:Cyanogen Bromide