Different types of calcitonin gene-related peptide-immunoreactive neurons in the guinea-pig stellate ganglion as revealed by triple-labelling immunofluorescence.
A triple-labelling immunofluorescence technique was used to study the patterns of coexistence of calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), and tyrosine hydroxylase (TH) in the guinea-pig stellate ganglion. CGRP-immunoreactive postganglionic neurons, which all were of the non-catecholaminergic type, could be divided into NPY-positive and NPY-negative populations. Sweat glands, which are a known target of CGRP-immunoreactive sympathetic neurons, exhibited exclusively fibers of the CGRP+/NPY- phenotype. Lack of coexistence of CGRP- and NPY-immunoreactivity was also observed in nerves within skeletal muscle, brown adipose tissue and hairy skin. The findings document a previously unknown diversity of CGRP-immunoreactive postganglionic sympathetic neurons, and indicate the existence of an addition, presently unidentified target of non-catecholaminergic, CGRP-immunoreactive sympathetic neurons.
Institute for Anatomy and Cell Biology I. University of Heidelberg, F.G.R.
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Kummer
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Heym
D002001:Adipose Tissue, Brown / Q000294:innervation / Q000378:metabolism; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009132:Muscles / Q000294:innervation / Q000378:metabolism; D009474:Neurons / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D009478:Neuropeptide Y / Q000276:immunology / Q000378:metabolism; D012867:Skin / Q000294:innervation / Q000378:metabolism; D012869:Skin Absorption; D013233:Stellate Ganglion / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D013545:Sweat Glands / Q000276:immunology / Q000294:innervation; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology / Q000378:metabolism
D009478:Neuropeptide Y; D014446:Tyrosine 3-Monooxygenase; D015740:Calcitonin Gene-Related Peptide