Abrogation of Fv-1 restriction by genome-deficient virions produced by a retrovirus packaging cell line.
The Fv-1b-mediated restriction of N-tropic retrovirus vector infection of BALB/3T3 cells was partially abrogated by prior infection with N-tropic murine leukemia virus. Likewise, abrogation of the Fv-1b restriction of N-tropic murine leukemia virus replication was accomplished by prior infection with genome-deficient virions produced by an N-tropic murine leukemia virus packaging cell line. The latter observation suggests that the Fv-1 target in genome-deficient virions abrogates Fv-1 restriction in the absence of any viral genome-directed processes.
Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
L R
LR
Boone
C L
CL
Innes
C K
CK
Heitman
D000818:Animals; D002460:Cell Line; D005796:Genes; D005822:Genetic Vectors; D009052:Leukemia Virus, Murine / Q000254:growth & development*; D051379:Mice / Q000235:genetics* / Q000382:microbiology; D008907:Mink; D009024:Morphogenesis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013045:Species Specificity; D014779:Virus Replication
D012194:RNA-Directed DNA Polymerase