[A multifactorial statistical analysis of a study of macromolecule interactions. Use of immunocomplexes and human alpha-2 macroglobulins].
Image processing of molecule electron microscopic views allows the observation of structural details which are randomly destroyed in a fraction of the images. The computation of average images reinforces significant details and weakens random variations provided that the image set is homogeneous. Before the computation of the average images the selection of homogeneous image population was carried out by the multiple correspondence analysis method. Two biological systems were used to demonstrate how much this method can help to understand protein-protein interactions: the topological localization of epitopes on a subunit of hemocyanin, and the intramolecular localization of proteases within human alpha 2-macroglobulin.
Faculté de pharmacie, Tours.
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Lamy
D000936:Antigen-Antibody Complex; D002918:Chymotrypsin / Q000737:chemistry; D006433:Hemocyanins / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D008956:Models, Chemical; D015999:Multivariate Analysis; D011485:Protein Binding; D000511:alpha-Macroglobulins / Q000737:chemistry
D000936:Antigen-Antibody Complex; D000511:alpha-Macroglobulins; D006433:Hemocyanins; D002918:Chymotrypsin
L'analyse statistique multifactorielle pour l'étude des interactions entre macromolécules. Application aux immunocomplexes et á l'alpha 2-macroglobuline humaine.