Reconstitution of a chloride-translocating ATPase from Aplysia californica gut.
Basolateral membranes of Aplysia foregut epithelia contain both a Cl(-)-stimulated ATPase activity and an ATP-dependent Cl- transport. The protein responsible for both of these biochemical activities (Cl- pump) can be solubilized and reconstituted into liposomes with the aid of the detergent digitonin. Proteoliposomal Cl- pump activity was inhibited by vanadate.
Department of Physiology, College of Medicine, University of Florida, Gainesville 32610.
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Gerencser
D000818:Animals; D001048:Aplysia; D001693:Biological Transport, Active; D015415:Biomarkers; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D018118:Chloride Channels; D002712:Chlorides / Q000493:pharmacokinetics*; D007422:Intestines / Q000187:drug effects / Q000201:enzymology*; D007473:Ion Channels / Q000187:drug effects / Q000201:enzymology*; D008565:Membrane Proteins / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology
D015415:Biomarkers; D018118:Chloride Channels; D002712:Chlorides; D007473:Ion Channels; D008565:Membrane Proteins; D014638:Vanadates