A solution for removal of resin from epoxy sections.
Development of a resin-dissolving solution for use at low alkali concentrations is described. Crown ether dissolved in dimethyl-sulfoxide produces a superbasic alkoxide anion. A five minute treatment resulted in complete resin removal from kidney biopsy specimens embedded in Epon 812. Specimens were well stained by Loeffler's methylene blue. Periodic acid-methenamine silver and Giemsa stains yielded good results. Application of PAS reaction and subsequent hematoxylin counterstaining was practicable for diagnosis.
Research Laboratory, Sakura Finetechnical Co., Tokyo, Japan.
T
T
Iwadare
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E
Harada
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S
Yoshino
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Arai
D000468:Alkalies; D004121:Dimethyl Sulfoxide; D004853:Epoxy Resins; D004988:Ethers, Cyclic; D006652:Histological Techniques; D006801:Humans; D007668:Kidney / Q000033:anatomy & histology; D013194:Staining and Labeling / Q000379:methods
D000468:Alkalies; D004853:Epoxy Resins; D004988:Ethers, Cyclic; D004121:Dimethyl Sulfoxide