A solid phase reverse transcriptase micro-assay for the detection of human immunodeficiency virus and other retroviruses in cell culture supernatants.
A simple and rapid solid-phase reverse transcriptase assay was developed based on the use of poly(rA):oligo(dT)12-18 as template primer immobilized on DEAE cellulose paper squares to detect human immunodeficiency virus (HIV) and/or other retroviruses in cell culture supernatants. It was found that PEG (per se) -up to 4% concentrations (w/v)--did not inhibit reverse transcriptase activity. Optimal conditions of the assay were determined. This solid-phase technique is much faster and more convenient than the methods described previously.
National Institute of Hygiene, Microbiological Research Group, Budapest.
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Somogyi
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Gyuris
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Földes
D002478:Cells, Cultured; D011232:Chemical Precipitation; D006678:HIV / Q000201:enzymology / Q000302:isolation & purification*; D011061:Poly A; D011071:Poly T; D011067:Poly dA-dT; D011092:Polyethylene Glycols; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012190:Retroviridae / Q000201:enzymology / Q000302:isolation & purification*
D011061:Poly A; D011071:Poly T; D011067:Poly dA-dT; C008950:poly A-T; D011092:Polyethylene Glycols; D012194:RNA-Directed DNA Polymerase