Expression of the murine interleukin-5 receptor on Xenopus laevis oocytes.
In this study we describe the use of Xenopus laevis oocytes for the detection of mRNA coding for a murine interleukin-5 (mI15) receptor. When injected with sucrose gradient fractionated polyA+ RNA derived from the murine 115-dependent pre B cell line B13, these oocytes could specifically bind 35S-methionine labeled mI15. A size of approximately 4000 nucleotides (25S) was estimated for the mRNA corresponding to the mIL5-binding activity. This binding was not blocked by a monoclonal antibody R52 specific for the MI15-receptor, suggesting that the oocytes express a different form of this receptor.
Devos
R
R
Roche Research Gent, Belgium.
Tavernier
J
J
Plaetinck
G
G
Van der Heyden
J
J
Rolink
A
A
Fiers
W
W
D000818:Animals; D002460:Cell Line; D015848:Interleukin-5 / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5; D011994:Recombinant Proteins / Q000378:metabolism; D014158:Transcription, Genetic; D014982:Xenopus laevis
D015848:Interleukin-5; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5; D011994:Recombinant Proteins; D011061:Poly A; D012313:RNA