Expression of the murine fibroblast growth factor 5 gene in the adult central nervous system.
The murine homolog of the fibroblast growth factor-5 (FGF-5) gene has been cloned, and the sequence of the gene's three exons has been determined. The murine gene and the previously isolated human FGF-5 gene are substantially homologous within the coding sequences and in upstream sequences, which contain an additional open reading frame. We have used a portion of the murine gene as probe to detect FGF-5 RNA in adult mouse tissues by both Northern blot and in situ hybridization methods. FGF-5 RNA is present at low levels in widely distributed areas of the central nervous system. Several loci of FGF-5 expression could be localized by in situ hybridization and include portions of the cerebral cortex, hippocampus, and thalamus. Neuronal expression accounts for at least some of the FGF-5 RNA synthesized in the central nervous system.
Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032.
O
O
Haub
B
B
Drucker
M
M
Goldfarb
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005346:Fibroblast Growth Factors / Q000235:genetics*; D015870:Gene Expression; D005796:Genes; D015698:Genomic Library; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D005346:Fibroblast Growth Factors; D012313:RNA; D004247:DNA