DNA sequence recognition by bleomycin.
The ability of bleomycin to cleave DNA fragments containing (TA)n and (GA)n/(CT)n inserts has been investigated. Bleomycin cuts efficiently after AT and GA though not at TA, AG and CT or TC. In the presence of ligands which modify DNA structure new cleavage products appear. It seems that the ability of bleomycin to discriminate between various sequences arises from their structural characteristics rather than merely from base-specific hydrogen bond contacts.
Department of Physiology & Pharmacology, University of Southampton, UK.
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Fox
D001482:Base Composition / Q000187:drug effects; D001483:Base Sequence / Q000187:drug effects; D001761:Bleomycin / Q000494:pharmacology*; D004247:DNA / Q000032:analysis / Q000187:drug effects*; D003609:Dactinomycin / Q000494:pharmacology; D004347:Drug Interactions; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008024:Ligands; D010957:Plasmids / Q000187:drug effects; D013329:Structure-Activity Relationship
D008024:Ligands; D001761:Bleomycin; D003609:Dactinomycin; D004247:DNA