Distribution in tissue sections of the human groEL stress-protein homologue.
A monoclonal antibody (ML30) raised against the 65 kDa heat-shock protein of mycobacteria showed widespread staining of sections from standard paraffin-embedded human tissues. The staining had a granular pattern and was particularly marked in cells with abundant mitochondria. Increased staining was observed in the synovial lining, histocytes and in the endothelium of reactive and rheumatoid synovium; it was also increased in the reactive lung alveolar lining. It is suggested that the antibody identifies an epitope in mitochondria of a protein homologous with the groEL heat-shock protein of bacteria.
Department of Histopathology, St. Mary's Hospital Medical School, London.
D J
DJ
Evans
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Norton
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Ivanyi
D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism* / Q000493:pharmacokinetics; D018834:Chaperonin 60; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000276:immunology / Q000378:metabolism* / Q000493:pharmacokinetics; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008168:Lung / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008928:Mitochondria / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D009166:Mycobacterium leprae / Q000276:immunology / Q000378:metabolism; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D018834:Chaperonin 60; D000939:Epitopes; D006360:Heat-Shock Proteins