Mouse ovarian factors with angiogenic activities: partial characterization.
Extracts of nonluteal mouse ovaries (Jcl: ICR strain) were assayed for neovascularization by implanting Elvax films, impregnated with test samples, on the lateral wall of the sheath of m. rectus abdominis in young adult mice of the same strain. Neovascularization occurred in a dose-dependent manner. Angiogenic activity was increased in extracts of ovaries from mice treated with follicle-stimulating hormone (FSH). The angiogenic activity was retained when the extract was heated to 100 degrees C for 15 min or adsorbed with activated charcoal. The ovarian extract was purified by affinity chromatography on a Con A-Sepharose 4B column. The adsorbed fraction possessed higher angiogenic activity. The ovarian extract was fractionated by ammonium sulfate precipitation. High angiogenic activity was found in the fraction collected between 20 to 50% saturation.
Department of Animal Science, Faculty of Agriculture, Kyoto University, Japan.
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Sato
D000818:Animals; D001681:Biological Assay; D001685:Biological Factors / Q000302:isolation & purification / Q000502:physiology*; D002846:Chromatography, Affinity; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology; D006358:Hot Temperature; D051379:Mice; D008813:Mice, Inbred ICR; D009389:Neovascularization, Pathologic / Q000503:physiopathology*; D010053:Ovary / Q000502:physiology*
D001685:Biological Factors; D005640:Follicle Stimulating Hormone