Overview and principles of in-situ hybridization.
In-situ hybridization is a technique that allows precise automated localization of DNA or RNA sequences in tissue preparations. The principles and kinetics of in-situ hybridization are presented. The technical considerations in various protocols used in in-situ hybridization are also reviewed.
Nakamura
R M
RM
Department of Pathology, Scripps Clinic and Research Foundation, La Jolla, CA 92037.
D001482:Base Composition; D004247:DNA / Q000235:genetics*; D005404:Fixatives; D007553:Isotope Labeling; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes; D009994:Osmolar Concentration; D010539:Permeability; D012313:RNA / Q000235:genetics*
D005404:Fixatives; D015341:Nucleic Acid Probes; D012313:RNA; D004247:DNA