Immunohistochemical analysis of p53 and HER-2/neu proteins in human tumors.
We examined samples of tumors of human breast, ovary, and colon of various degrees of malignancy for the expression of p53 protein, using a panel of anti-p53 antibodies and peroxidase immunohistochemistry. Of 66 tumor cases (24 cases of ovarian carcinoma, 23 cases of colon adenocarcinoma, and 19 cases of breast carcinoma), 36 (53%) showed high levels of expression of p53 using a human-specific antibody, and 16 (24%) showed high expression of a mutant form of p53. In the mutant p53-positive breast tumor samples, six (86%) were positive for HER-2/neu reactivity, compared with colon (0/4) and ovarian tumors (1/5). The pattern of p53 intracellular localization and tissue distribution, and the relationship between the expression of mutant p53 and cell differentiation, were also examined; poorly differentiated cells showed either overexpression of p53 or higher levels of mutant p53 in comparison with more normal cells.
Chang
K
K
Ultrastructural Cytochemistry Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Ding
I
I
Kern
F G
FG
Willingham
M C
MC
D001943:Breast Neoplasms / Q000737:chemistry; D003110:Colonic Neoplasms / Q000737:chemistry; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D009154:Mutation; D009369:Neoplasms / Q000737:chemistry*; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D010051:Ovarian Neoplasms / Q000737:chemistry; D018719:Erb-b2 Receptor Tyrosine Kinases; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D009856:Oncogene Proteins, Viral; D018719:Erb-b2 Receptor Tyrosine Kinases; D016159:Tumor Suppressor Protein p53