Isolation of recombinant fragments of the major outer-membrane protein of Chlamydia trachomatis: their potential as subunit vaccines.
Recombinant fragments of the major outer-membrane protein (MOMP) of Chlamydia trachomatis, expressed at high levels in Escherichia coli, were isolated and purified. Antisera to the recombinant proteins reacted preferentially with overlapping synthetic peptides covering the immunoaccessible variable segments of MOMP. These sera also reacted in a species-specific manner with the surface of intact infectious elementary bodies, and in a Chlamydia genus-specific manner in assays using denatured or bound chlamydial antigens. The ability of recombinant MOMP preparations to elicit antibody to the surface of chlamydial elementary bodies raises the possibility that these proteins may be useful for chlamydial vaccine development.
Department of Microbiology, University of Southampton Medical School, UK.
J W
JW
Conlan
S
S
Ferris
I N
IN
Clarke
M E
ME
Ward
D000818:Animals; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology* / Q000302:isolation & purification; D002690:Chlamydia Infections / Q000517:prevention & control; D002692:Chlamydia trachomatis / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D010449:Peptide Mapping; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic