Comparison of channels formed by poly C9, C5b-8 and the membrane attack complex of complement.
The channels formed by poly C9, C5b-8 and C5b-9 were examined using the liposome swelling assay. By plotting the relative rate of swelling of C5b-8-containing liposomes vs the molecular weight of the sugar solute and by applying the Renkin equation, the size of the C5b-8 channel was estimated to be 1.5 mm radius. As increasing amounts of C9 were added during the formation of C5b-9, in C8:C9 ratios of 1:1, 1:2, 1:6 and 1:12, the size of the function channel increased. Poly C9 had a pore that was somewhat larger than C5b-9 at a C8:C9 ratio of 1:12. Using molecular sieving experiments with four different iodinated protein size markers, the channel diameter of poly C9 was estimated at between 90 and 100 A. Monoclonal antibodies to different complement proteins were added to the liposomes to see which might inhibit the channels. C5b-8 containing liposomes could be inhibited by antibodies to C8. Liposomes containing C5b-9 could be inhibited slightly by antibodies to C9 and most strongly by antibodies to the neoantigen of poly C9.
Zalman
L S
LS
Research Institute of Scripps Clinic, Department of Immunology, La Jolla, CA 92037.
Müller-Eberhard
H J
HJ
D000911:Antibodies, Monoclonal; D003186:Complement C9 / Q000276:immunology / Q000648:ultrastructure*; D015938:Complement Membrane Attack Complex / Q000276:immunology / Q000648:ultrastructure*; D003165:Complement System Proteins / Q000276:immunology / Q000648:ultrastructure*; D006801:Humans; D066298:In Vitro Techniques; D007473:Ion Channels / Q000648:ultrastructure*; D008081:Liposomes; D046911:Macromolecular Substances; D010539:Permeability; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D003186:Complement C9; D015938:Complement Membrane Attack Complex; D007473:Ion Channels; D008081:Liposomes; D046911:Macromolecular Substances; C042295:complement C5b-8 complex; D003165:Complement System Proteins