Immunoprecipitation of herpes simplex virus polypeptides with human sera is related to their ELISA titre.
Out of 485 human sera tested by neutralization and enzyme immunoassay 13 were negative in both tests, while a positive correlation was found in 457 samples (94.3%). The rest of sera (3.2%) showed discordant results. Selected sera were examined, in addition, by Western blot and immunoprecipitation assays with the aim to analyse the precipitation profile of discordant sera and correlate the ELISA titre with the precipitation profiles. High titre sera precipitated the main glycoproteins (gC, gB, gE, gD), the capsid polypeptides (VP 5, VP 19, VP 21, VP 22) and several other structural and nonstructural proteins. The ability of sera to precipitate viral structural proteins was related to their ELISA titre rather than to their neutralizing activity.
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czecho-Slovakia.
M
M
Murányiová
J
J
Rajcáni
M
M
Krivjanská
J
J
Matis
J
J
Pogády
D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007122:Immunoelectrophoresis; D008875:Middle Aged; D009500:Neutralization Tests; D011233:Precipitin Tests; D018139:Simplexvirus / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins