1H-NMR and HPLC studies of the changes involved in volume regulation in the muscle fibres of the crab, Hemigrapsus edwardsi.
1. The process of cell volume readjustment, during adaptation to salinity changes, in muscle fibres of the euryhaline New Zealand shore crab, Hemigrapsus edwardsi, involve large changes in the amounts of free amino acid. 2. These are taurine, proline, alanine, arginine, glutamic acid, glycine and serine. 3. These changes may be quantified by High Performance Liquid Chromatography, and qualitatively demonstrated by proton nuclear magnetic resonance spectroscopy.
Department of Physiology, University of Otago Medical School, Dunedin, New Zealand.
J J
JJ
Bedford
R A
RA
Smith
M
M
Thomas
J P
JP
Leader
D000222:Adaptation, Physiological; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D003386:Brachyura / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D006458:Hemolymph / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009132:Muscles / Q000737:chemistry*; D011522:Protons; D012623:Seawater; D012965:Sodium Chloride / Q000032:analysis
D000596:Amino Acids; D011522:Protons; D012965:Sodium Chloride