Heterogeneity of Drosophila nicotinic acetylcholine receptors: SAD, a novel developmentally regulated alpha-subunit.
Two genes, ard and als, are known to encode subunits of the nicotinic acetylcholine receptor (nAChR) in Drosophila. Here we describe the isolation of cDNA clones encoding a novel member (SAD, or alpha 2) of this receptor protein family. The deduced amino acid sequence displays high homology to the ALS protein and shares structural features with ligand binding nAChR alpha-subunits. Sad transcripts accumulate during major periods of neuronal differentiation and, in embryos, are localized in the central nervous system. Expression of SAD cRNA in Xenopus oocytes generates cation channels that are gated by nicotine. These data indicate heterogeneity of nAChRs in Drosophila.
ZMBH, Universität Heidelberg, FRG.
E
E
Sawruk
P
P
Schloss
H
H
Betz
B
B
Schmitt
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D015698:Genomic Library; D046911:Macromolecular Substances; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009865:Oocytes / Q000502:physiology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011978:Receptors, Nicotinic / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014981:Xenopus
D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D011978:Receptors, Nicotinic; D012313:RNA; D004247:DNA