Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation.
A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.
Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010.
J
J
Singer-Sam
M
M
Grant
J M
JM
LeBon
K
K
Okuyama
V
V
Chapman
M
M
Monk
A D
AD
Riggs
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D015226:Dinucleoside Phosphates; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D008297:Male; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014960:X Chromosome
D004247:DNA; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D015226:Dinucleoside Phosphates; D015345:Oligonucleotide Probes; D010735:Phosphoglycerate Kinase; C015772:CpG dinucleotide