Double immunohistochemical detection of transmitter phenotype of proliferating cells using bromodeoxyuridine.
We describe a new method that can determine transmitter phenotype of proliferating nerve cells at a given age. The procedure is based on indirect sequential double antigen immunofluorescence histochemistry for transmitter-synthesizing enzymes (glutamic acid decarboxylase and tyrosine hydroxylase) and the thymidine analogue, bromodeoxyuridine. The method permits simple, rapid, and effective anatomical detection, and promises to reduce certain limitations inherent in a combination with tritiated thymidine autoradiography. Employing this technique, we observed that many striatal cells expressing gamma-aminobutyric acid (GABA) and nigral cells expressing dopamine undergo the final mitosis at embryonic days 13-14 in the rat.
Department of Anatomy and Neurobiology, College of Medicine, University of Tennessee, Memphis 38163.
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T
Kono
M
M
Takada
J Y
JY
Wu
S T
ST
Kitai
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D001973:Bromodeoxyuridine; D002455:Cell Division; D005260:Female; D005455:Fluorescent Antibody Technique; D005865:Gestational Age; D005968:Glutamate Decarboxylase / Q000032:analysis; D007150:Immunohistochemistry; D018377:Neurotransmitter Agents / Q000096:biosynthesis*; D010641:Phenotype; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D013378:Substantia Nigra / Q000166:cytology / Q000196:embryology / Q000201:enzymology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D018377:Neurotransmitter Agents; D014446:Tyrosine 3-Monooxygenase; D005968:Glutamate Decarboxylase; D001973:Bromodeoxyuridine