Mapping of a retinoic acid-responsive element in the promoter region of the complement factor H gene.
Retinoic acid receptors are members of the steroid/thyroid hormone receptor superfamily. Pursuant to the discovery that dexamethasone increases complement factor H expression, we examined the effects of retinoic acid on this gene. Both H mRNA and protein levels are increased by retinoic acid in L cells. Using the luciferase reporter gene system we have identified a region of the H promoter required for the retinoic acid response. This region contains an imperfect palindrome of the TGACC motif, present in thyroid hormone and estrogen-responsive elements. We demonstrate specific binding of the retinoic acid receptor beta to this sequence of the H gene by DNA-protein gel retardation assay. Therefore, these studies extend the sphere of influence of the retinoids to complement, an intrinsic component of the humoral immune system.
Muñoz-Cánoves
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Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037.
Vik
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DP
Tack
B F
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D000818:Animals; D001483:Base Sequence; D003180:Complement C3b Inactivator Proteins / Q000235:genetics*; D017242:Complement Factor H; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes / Q000187:drug effects; D007700:Kinetics; D007739:L Cells / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology*
D003180:Complement C3b Inactivator Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014212:Tretinoin; D012313:RNA; D017242:Complement Factor H