The role of CD44, CD45, CD45RO, CD46 and CD55 as potential anti-adhesion molecules involved in the binding of human tonsillar T cells to phorbol 12-myristate 13-acetate-differentiated U-937 cells.
In this study, we have shown that human tonsillar T cells adhere to phorbol 12-myristate 13-acetate(PMA)-differentiated U-937 cells. To examine the molecular mechanisms involved, the effect of a panel of monoclonal antibodies upon this adhesion was assessed in a quantitative binding assay. Antibodies against LFA-1 and ICAM-1 inhibited binding, directly implicated these molecules in T cell-PMA-induced U-937 adhesion. Furthermore, the adhesion was magnesium but not calcium dependent. Of the remaining antibodies that were tested, none of those against CD2, LFA-3, Mac-1, p150,95, CD43, CD45RA or CD56 affected binding. However, antibodies against CD44, CD45, CD45RO, CD46 and CD55 enhanced binding suggesting an anti-adhesive role for these molecules during U-937-T cell interaction.
Bland-Sutton Institute of Pathology, University College and Middlesex School of Medicine, London, GB.
P D
PD
King
A H
AH
Batchelor
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P
Lawlor
D R
DR
Katz
D000938:Antigen-Presenting Cells / Q000166:cytology*; D015703:Antigens, CD / Q000502:physiology*; D000943:Antigens, Differentiation / Q000502:physiology; D018958:CD55 Antigens; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D006649:Histocompatibility Antigens / Q000502:physiology; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D017493:Leukocyte Common Antigens; D008264:Macrophages / Q000166:cytology; D008274:Magnesium / Q000494:pharmacology; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000502:physiology; D008854:Microscopy, Electron; D014066:Palatine Tonsil / Q000166:cytology; D016201:Receptors, Lymphocyte Homing; D013601:T-Lymphocytes / Q000166:cytology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015703:Antigens, CD; D000943:Antigens, Differentiation; C497857:CD46 protein, human; D018958:CD55 Antigens; D015815:Cell Adhesion Molecules; D006649:Histocompatibility Antigens; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D016201:Receptors, Lymphocyte Homing; D017493:Leukocyte Common Antigens; D008274:Magnesium; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium