Productive infection of T-helper lymphocytes with feline immunodeficiency virus is accompanied by reduced expression of CD4.
An antigen-specific feline T-lymphocyte cell line (Q201) was generated and infected in vitro with the feline immunodeficiency virus (FIV). Syncytium formation and the release of the viral core protein p24 into culture fluid were accompanied by a reduction in expression of the CD4 surface antigen. The reduction in CD4 expression was transient, the resulting persistently infected population of cells expressing levels of CD4 comparable to those observed prior to infection. Persistently infected cells gradually lost expression of major histocompatibility antigen (MHC) class II while maintaining pre-infection levels of expression of CD4, MHC class I, CD18 or CD29.
Department of Veterinary Pathology, University of Glasgow Veterinary School, Scotland, UK.
B J
BJ
Willett
M J
MJ
Hosie
T H
TH
Dunsford
J C
JC
Neil
O
O
Jarrett
D000818:Animals; D015153:Blotting, Western; D015704:CD4 Antigens / Q000032:analysis*; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology*; D002415:Cats; D002455:Cell Division / Q000502:physiology; D015870:Gene Expression; D015726:Giant Cells / Q000382:microbiology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D016089:Immunodeficiency Virus, Feline / Q000502:physiology*; D007700:Kinetics; D014407:Tumor Cells, Cultured; D014758:Viral Core Proteins / Q000032:analysis; D014779:Virus Replication / Q000502:physiology
D015704:CD4 Antigens; D000949:Histocompatibility Antigens Class II; D014758:Viral Core Proteins