Characterization of adhesion zones in E. coli cells.
After plasmolysis of Escherichia coli cells, the adhesion zones were characterized using the cytochemical PTA and SP procedures which stain peptidoglycan and lipopolysaccharides (LPS) respectively. A PTA-stained layer was detected at the adhesion sites. This layer was visualized irrespective of the electron microscopy procedure used. Also, using SP staining an outer membrane in which LPS molecules were asymmetrically distributed, was observed.
Unité associée du CNRS, Biochimie Moléculaire et cellulaire Université Paris-sud, Orsay, France.
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M
Leduc
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C
Frehel
D001422:Bacterial Adhesion / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D004926:Escherichia coli / Q000378:metabolism / Q000648:ultrastructure*; D008070:Lipopolysaccharides / Q000378:metabolism; D008854:Microscopy, Electron; D010457:Peptidoglycan / Q000378:metabolism; D013194:Staining and Labeling
D008070:Lipopolysaccharides; D010457:Peptidoglycan