The non-osteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteoblastic cells by recombinant human bone morphogenetic protein-2.
The possibility that the non-osteogenic mouse pluripotent cell line, C3H10T1/2 (10T1/2), could be induced to differentiate into osteogenic cells by various hormones and cytokines was examined in vitro. Of a number of agents tested, recombinant human bone morphogenetic protein-2 (rhBMP-2) and retinoic acid induced alkaline phosphatase (ALP) activity in 10T1/2 cells. rhBMP-2 also induced mRNA expression of ALP in the cells. Dexamethasone, 1 alpha, 25-dihydroxyvitamin D3, transforming growth factor-beta 1 and insulin-like growth factor-I did not stimulate ALP activity. Treatment with rhBMP-2 greatly induced cAMP production in response to parathyroid hormone in 10T1/2 cells. No ALP activity was induced in NIH3T3 fibroblasts treated with rhBMP-2 or retinoic acid. These results indicate that 10T1/2 cells have a potential to differentiate into osteogenic cells under the control of BMP-2.
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
T
T
Katagiri
A
A
Yamaguchi
T
T
Ikeda
S
S
Yoshiki
J M
JM
Wozney
V
V
Rosen
E A
EA
Wang
H
H
Tanaka
S
S
Omura
T
T
Suda
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D019485:Bone Morphogenetic Proteins; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D010281:Parathyroid Hormone / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology*; D012313:RNA / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000494:pharmacology; D019379:Teriparatide
D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D011506:Proteins; D011994:Recombinant Proteins; D019379:Teriparatide; D012313:RNA; D000242:Cyclic AMP; D000469:Alkaline Phosphatase; D015056:1-Methyl-3-isobutylxanthine