[Detection of the measles virus genome in the peripheral blood lymphocytes of patients with chronic and acute glomerulonephritis].
The presence of the measles (rubeola) virus genome was searched for in the lymphocytes from the peripheral blood of patients suffering from the acute (16 persons) or chronic (164 persons) glomerulonephritis. Dot hybridization technique with the plasmid borne probes to the measles viral genes NP, P and H have been used for the search. The measles viral genome has been detected in 58% of lymphocytes from the patients with the chronic glomerulonephritis and in 50% of lymphocytes from the patients suffering from the acute form of the disease. The genome was not found in the material from the control group including donors and traumatology ward patients. 25 samples of lymphocytes from the patients with the chronic glomerulonephritis contained the RNA that was not hybridizable with the viral genes probes by dot hybridization technique, thus containing no genes homologous to parotitis viral genes. The average titer of anti-measles antibodies in the serum from patients with chronic glomerulonephritis the lymphocytes of which contained the measles viral genome was 1:304, while it was 1:154 for patients with the negative probes. The average anti-measles antibodies titers are the same (1:166 and 1:142) for analogical groups of patients with acute form of disease.
Bogomolova
N N
NN
Chaplygina
N M
NM
Ataullakhanov
A I
AI
Burgasova
M P
MP
Nevraeva
E G
EG
Filimonova
R G
RG
D000208:Acute Disease; D000914:Antibodies, Viral / Q000097:blood; D002908:Chronic Disease; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D005921:Glomerulonephritis / Q000097:blood / Q000382:microbiology*; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D008459:Measles virus / Q000235:genetics* / Q000276:immunology; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics
D000914:Antibodies, Viral; D004279:DNA, Viral; D012313:RNA; D004247:DNA
Vyiavlenie genoma virusa kori v limfotsitakh perifericheskoĭ krovi bol'nykh khronicheskim i ostrym glomerulonefritom.