Antigenic conservation of primary structural regions of S-adenosylmethionine synthetase.
Although the physical and kinetic properties of S-adenosylmethionine (AdoMet) synthetases from different sources are quite different, it appears that these enzymes have structurally or antigenically conserved regions as demonstrated by studies with AdoMet synthetase specific antibodies. Polyclonal anti-human lymphocyte AdoMet synthetase crossreacted with enzyme from rat liver (beta isozyme), Escherichia coli and yeast. In addition, polyclonal anti-E. coli enzyme and antibodies to synthetic peptides copying several regions of the yeast enzyme reacted with the human gamma and rat beta isozymes. Antibodies to yeast SAM1 encoded protein residues 6-21, 87-113 and 87-124 inhibited the activity of human lymphocyte AdoMet synthetase, while antibodies to residues 272-287 had no effect on the enzyme activity. Our results suggest that these conserved regions may be important in enzyme activity.
Kotb
M
M
Veterans Administration Medical Center, Memphis, TN 38104.
Geller
A M
AM
Markham
G D
GD
Kredich
N M
NM
De La Rosa
J
J
Beachey
E H
EH
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology; D007527:Isoenzymes / Q000235:genetics / Q000276:immunology; D008099:Liver / Q000201:enzymology; D008716:Methionine Adenosyltransferase / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis; D011817:Rabbits / Q000276:immunology; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012689:Sequence Homology, Nucleic Acid; D014166:Transferases / Q000235:genetics*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007527:Isoenzymes; D010455:Peptides; D014166:Transferases; D008716:Methionine Adenosyltransferase