Partition of protein and DNA during cytokinesis in human breast cancer cell lines.
Three human breast cancer cell lines (HTB-126, MDA-231, and HTB-122) with DNA index (DI) values between 1.26 and 1.72 were analysed together with a diploid mouse embryonal carcinoma cell line (PCC3) by a TV-video time-lapse technique (pedigree analysis). Cytochemical parameters (DNA and proteins) were studied in individual cells in a rapid scanning microspectrophotometer. Post-mitotic sister cell pairs were analysed after Feulgen-naphthol-yellow staining. The DI values of the cell lines were selected to reflect various well-known clinical ploidy entities differing in malignancy potentials. A mitotic disturbance of the partition of DNA and protein to daughter cells was found in particular in MDA-231 closest to the triploid DNA modal value (DI = 1.37). Duration of mitosis was considerably longer in the near triploid line compared to the other lines. The MDA-231 line was also least sensitive to suboptimal growth conditions. This report calls attention to a possible causality between mitotic error and intraclonal genotype and cell mass heterogeneity.
Department of Tumour Pathology, Karolinska Institute and Hospital, Stockholm, Sweden.
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Sennerstam
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Auer
D000782:Aneuploidy; D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002455:Cell Division; D002470:Cell Survival; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D005838:Genotype; D006801:Humans; D051379:Mice; D008938:Mitosis; D009363:Neoplasm Proteins / Q000032:analysis*; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured / Q000166:cytology
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins