A rapid, sensitive histochemical stain for myelin in frozen brain sections.
A staining technique is described whereby frozen brain sections are incubated in a vehicle containing gold chloride. After 2-4 hr in this solution, both large myelinated bundles and fine individually myelinated fibers are darkly stained. Advantages of this technique over conventional myelin stains include speed, sensitivity, metachromatic staining, and compatibility with formalin-fixed and frozen cut sections. Possible histochemical mechanisms are discussed.
Department of Otolaryngology, University of Virginia Medical Center, Charlottesville 22908.
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D000818:Animals; D001921:Brain / Q000378:metabolism*; D005615:Freezing; D006046:Gold; D017612:Gold Compounds; D006651:Histocytochemistry / Q000379:methods*; D009186:Myelin Sheath / Q000378:metabolism*; D014677:Pharmaceutical Vehicles / Q000494:pharmacology; D012680:Sensitivity and Specificity; D012996:Solutions; D013194:Staining and Labeling; D013997:Time Factors
D017612:Gold Compounds; D014677:Pharmaceutical Vehicles; D012996:Solutions; C038016:gold chloride; D006046:Gold