Subpopulations of lymphocytes in connective tissue from adolescents with periodontal disease.
Mononuclear cell populations were studied in gingival biopsy specimens from adolescents (n = 10) with at least one periodontal pocket with an increased probing depth (greater than 5 mm). The marginal bone loss was measured on radiographs, subgingival plaque samples were collected from the lesions, and the microbial flora was identified. Specimens from gingivitis lesions (n = 5) were used as controls. The mononuclear cell populations in the specimens were detected by using monoclonal antibodies defining functional T-lymphocyte subpopulations, B lymphocytes, and monocytes. All gingival specimens from patients with increased probing depth showed large lymphocyte infiltrates, most of which were CD 3-antigen-positive cells (T lymphocytes). Few (2%) infiltrating T cells expressed receptors for interleukin-2. B cells were detected in most specimens from the periodontitis group and varied from less than 1% to 21%. This study indicates that lesions in adolescents with early signs of periodontitis are characterized predominantly by T-cell lesions with relatively few cell aggregates of B cells present.
Department of Pedodontics, School of Dentistry and Clinical Immunology, Huddinge Hospital, Karolinska Institute, Stockholm, Sweden.
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Dahllöf
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Axiö
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Sundqvist
D000188:Actinobacillus / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D001439:Bacteroides / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D003238:Connective Tissue / Q000473:pathology; D000939:Epitopes / Q000032:analysis*; D005260:Female; D005881:Gingiva / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D010510:Periodontal Diseases / Q000382:microbiology / Q000473:pathology*; D010512:Periodontal Index; D013601:T-Lymphocytes / Q000473:pathology; D006377:T-Lymphocytes, Helper-Inducer / Q000473:pathology
D000911:Antibodies, Monoclonal; D000939:Epitopes