Lack of correlation in the interferon-induced small Mw 2-5A synthetase mRNA levels and sensitivity of two L cell sublines to the antiviral and priming effects of interferon.
MuIFN-alpha/beta at a concentration of 0.5 IU/ml exerted both antiviral and priming effects in the B, but not in the M subline of L929 cells. This concentration of IFN did not affect the small size 2-5A synthetase mRNA levels in either sublines, as determined by a Northern blot technique. Pretreatment with 5 or 50 IU/ml IFN resulted in more pronounced antiviral and priming effects in the L929B than in the L929M cells. However, the enhancements of the 2-5A synthetase mRNA levels due to the pretreating IFN dose were similar in both sublines.
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Institute of Microbiology, A. Szent-Györgyi Medical University, Szeged, Hungary.
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D000818:Animals; D002642:Chick Embryo; D005347:Fibroblasts; D007372:Interferons / Q000494:pharmacology*; D007739:L Cells; D008025:Ligases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development
D012333:RNA, Messenger; D007372:Interferons; D008025:Ligases