Evidence for the expression of a glucuronic acid-containing epitope in the central nervous system of two insects (Calliphora vicina, Diptera; Tenebrio molitor, Coleoptera).
The monoclonal antibody CAF-I recognises a glucuronic acid-containing epitope present on various acidic glycosphingolipids of Calliphora vicina. Immunohistochemistry was performed on CAF-I-labelled whole-mount preparations of the central nervous system, visualised by peroxidase-conjugated second antibody. A differential, temporal and spatial expression of this epitope in metamorphosing nervous tissue was outlined, that apparently characterises homologous neuronal populations in two phylogenetically distinct holometabolous insects, i.e. Calliphora vicina and Tenebrio molitor. Implications for a functional interpretation of insect glyco(sphingo)lipids in tissue development are discussed.
Institut für Angewandte Zoologie, Universität Bonn, F.R.G.
O
O
Breidbach
R D
RD
Dennis
M
M
Keller
H
H
Wiegandt
D000818:Animals; D000911:Antibodies, Monoclonal; D002490:Central Nervous System / Q000254:growth & development / Q000378:metabolism*; D001517:Coleoptera / Q000254:growth & development / Q000378:metabolism*; D004175:Diptera / Q000254:growth & development / Q000378:metabolism*; D000939:Epitopes; D005965:Glucuronates / Q000276:immunology / Q000378:metabolism*; D020723:Glucuronic Acid; D006028:Glycosphingolipids / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D007150:Immunohistochemistry; D007814:Larva
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005965:Glucuronates; D006028:Glycosphingolipids; D020723:Glucuronic Acid