Stimulation and priming of human neutrophils by granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor: qualitative and quantitative differences.
Granulocyte colony-stimulating factor(G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) increased neutrophil C3bi-receptor expression and adherence and rapidly (less than 10 min) primed neutrophils to enhanced O2- release and membrane depolarization stimulated by chemotactic peptide. Direct triggering of O2- release in suspended neutrophils was also provoked by GM-CSF but not by G-CSF. GM-CSF-induced O2- release was inhibited by cyclic AMP agonists and cytochalasin B. The biological activity was greater in non-glycosylated GM-CSF than in glycosylated GM-CSF, whereas it was identical in glycosylated and non-glycosylated G-CSFs. Direct stimulation and priming by GM-CSF were consistently greater than those by G-CSF and the combined addition of the optimal concentrations of G-CSF and GM-CSF resulted in the effects of GM-CSF alone. These findings indicate that the effects of G-CSF and GM-CSF on neutrophil functions are qualitatively and quantitatively different from each other.
Clinical Research Institute, National Medical Center, Tokyo, Japan.
A
A
Yuo
S
S
Kitagawa
A
A
Ohsaka
M
M
Saito
F
F
Takaku
D000527:Alprostadil / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D000242:Cyclic AMP / Q000502:physiology; D003571:Cytochalasin B / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*; D011951:Receptors, Complement / Q000378:metabolism; D017463:Receptors, Complement 3b; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D013481:Superoxides; D016179:Granulocyte Colony-Stimulating Factor; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000242:Cyclic AMP; D000527:Alprostadil