Regional distribution of DNA and RNA in rat brain: a sensitive determination using high-performance liquid chromatography with electrochemical detection.
DNA and RNA contents in 20 brain regions or nuclei of the rat were determined by a highly sensitive method using high-performance liquid chromatography with electrochemical detection. The high DNA and RNA contents were found in the hypothalamic nuclei, especially the median eminence-arcuate nucleus. These results may be available for the preparation of nucleic acids as the regional control.
Nakahara
T
T
Department of Chemistry, Faculty of Science, Kyushu University, Fukuoka, Japan.
Hirano
M
M
Matsumoto
T
T
Kuroki
T
T
Tatebayashi
Y
Y
Tsutsumi
T
T
Nishiyama
K
K
Ooboshi
H
H
Nakamura
K
K
Yao
H
H
D000679:Amygdala / Q000737:chemistry; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000032:analysis*; D006624:Hippocampus / Q000737:chemistry; D007031:Hypothalamus / Q000737:chemistry; D008125:Locus Coeruleus / Q000737:chemistry; D008297:Male; D009830:Olfactory Bulb / Q000737:chemistry; D012313:RNA / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012688:Septum Pellucidum / Q000737:chemistry; D014018:Tissue Distribution
D012313:RNA; D004247:DNA