Characterization of type IV collagen NC1 monomers and Goodpasture antigen in human renal basement membranes.
Monomeric subunits of the globular domain of type IV collagen from human renal basement membrane were isolated and characterized. The monomers, M24, M26, M28+, and M28 , which have been identified previously in human glomerular basement membrane, were characterized by amino acid analysis, amino-terminal sequencing, and electrophoretic mobility. The results indicate that M24 and M26 are derived from alpha 1(IV) and alpha 2(IV) collagen chains, respectively. Amino-terminal sequencing revealed that M28+, and M28 correspond to the globular domain of novel collagen chains. M28 has been characterized as the principal target antigen in Goodpasture's syndrome and antiglomerular basement membrane (GBM) nephritis, and both M28 species are absent from the GBM in Alport familial nephritis. The cationic charge of M28 appears to be a consequence of a relatively high concentration of basic amino acids when compared with other monomers. Previous studies of bovine GBM have demonstrated chains with amino-terminal sequence homology to M28+ and M28 .
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455.
R J
RJ
Butkowski
G Q
GQ
Shen
J
J
Wieslander
A F
AF
Michael
A J
AJ
Fish
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification; D001324:Autoantigens / Q000302:isolation & purification*; D001485:Basement Membrane / Q000032:analysis* / Q000276:immunology; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000302:isolation & purification*; D024141:Collagen Type IV; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000302:isolation & purification*; D006801:Humans; D007678:Kidney Glomerulus / Q000032:analysis* / Q000276:immunology; D008969:Molecular Sequence Data
D000596:Amino Acids; D001324:Autoantigens; D024141:Collagen Type IV; D000939:Epitopes; C057112:type IV collagen alpha3 chain; D003094:Collagen