Determination of selected organochlorine pesticides and polychlorinated biphenyls in human serum.
A method is presented that can be used to determine the residue level of certain chlorinated pesticides and polychlorinated biphenyls (as Aroclor 1260) in serum. The method involves the following: (1) extraction of denatured serum with organic solvents; (2) elution of the organic extract through micro-Florisil columns to obtain two fractions; (3) acid treatment of the less polar Florisil fraction and its subsequent elution through deactivated silica gel to obtain two fractions; and (4) analysis of all three fractions using gas-liquid chromatography with electron capture detection. The method produced in vitro recoveries for 10 pesticides spiked in the range of 1-10.7 ppb of 50.4% to 121.6%, and in the range of 4.98-21 ppb, recoveries ranged from 47.7 to 112.6. In vivo "recoveries" of Aroclor 1260 averaged 104.8% and 92.3% for concentration levels of approximately 10 and approximately 30 ppb, respectively. The method could not be compared with the more commonly used hexane extraction technique because of the deleterious effect these extracts had on the gas chromatographic system.
Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, Georgia 30333.
V W
VW
Burse
S L
SL
Head
M P
MP
Korver
P C
PC
McClure
J F
JF
Donahue
L L
LL
Needham
D002623:Chemistry Techniques, Analytical / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods; D003634:DDT / Q000097:blood; D003633:Dichlorodiphenyl Dichloroethylene / Q000097:blood; D004781:Environmental Exposure; D006581:Hexachlorobenzene / Q000097:blood; D001556:Hexachlorocyclohexane / Q000097:blood; D006586:Hexanes; D006801:Humans; D007306:Insecticides / Q000097:blood*; D017633:Magnesium Silicates; D011078:Polychlorinated Biphenyls / Q000097:blood*; D058428:Silica Gel; D012824:Silicic Acid; D012822:Silicon Dioxide
D006586:Hexanes; D007306:Insecticides; D017633:Magnesium Silicates; C005013:Florisil; D012824:Silicic Acid; D003633:Dichlorodiphenyl Dichloroethylene; D006581:Hexachlorobenzene; D001556:Hexachlorocyclohexane; D058428:Silica Gel; D012822:Silicon Dioxide; D003634:DDT; D011078:Polychlorinated Biphenyls; C023888:beta-hexachlorocyclohexane