Characterization of monoclonal antibodies to young-adult worms of Angiostrongylus cantonensis.
Three IgG1 and one IgG2a monoclonal antibodies were obtained by fusion of NS-1 cells with spleen cells of BALB/C mice immunized with young-adult worm antigen of A. cantonensis. These 4 monoclonal antibodies were specific to A. cantonensis without cross-reactivity to any antigens of Toxocara canis, Ascaris suum, Clonorchis sinensis, Dirofilaria immitis, Paragonimus westermani, Gnathostoma spinigerum, Strongyloides stercoralis and Anisakis spp. detected by ELISA. The affinity constants of monoclonal antibodies specific to young-adult worms antigen of A. cantonensis were in the range of 10(7) M-1 to 10(9) M-1. The immunoblots showed that the 4 monoclonal antibodies recognized epitope on molecular weight of 204 kiloDaltons. They will be utilized to purify their corresponding antigen by an immunoaffinity column for the specific immunodiagnosis.
Chang
J H
JH
Department of Parasitology, National Cheng Kung University Medical College, Tainan, Taiwan, ROC.
Yen
C M
CM
Chen
E R
ER
D000801:Angiostrongylus / Q000276:immunology*; D000818:Animals; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D009349:Nematode Infections / Q000175:diagnosis
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000939:Epitopes