Lassa virus glycoproteins: antigenic and immunogenic properties of synthetic peptides to GP1.
Synthetic peptides corresponding to predicted Lassa virus GP1 glycoprotein B-epitopes were used to study the antigenicity and immunogenicity of the protein. ELISA results showed that guinea pig polyclonal anti-Lassa virus serum bound effectively to peptides corresponding to amino acid residues 119-133 and 164-176 of the GP1 protein. Essentially it did not react to a peptide corresponding to GP1 amino acid residues 234-256. Sera obtained against peptides representing amino acid residues 119-133 and 164-176 reacted with inactivated purified Lassa virus.
Department of Special Pathogens, Byelorussian Research Institute of Epidemiology and Microbiology, Minsk, U.S.S.R.
A G
AG
Krasko
A B
AB
Moshnikova
A T
AT
Kozhich
L D
LD
Tchikin
V T
VT
Ivanov
A S
AS
Vladyko
I S
IS
Lukashevich
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007836:Lassa virus / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D007106:Immune Sera; D010455:Peptides; D014759:Viral Envelope Proteins; C066508:glycoprotein gp1, lassa virus