Relevance of anti-nef antibody detection as an early serologic marker of human immunodeficiency virus infection.
The relevance, previously suggested by some authors, of anti-nef antibody detection as an early marker of human immunodeficiency virus (HIV) infection was examined with a sensitive liquid phase radioimmunoassay by investigating: (1) the kinetics of appearance of anti-nef-antibodies in a set of 77 longitudinal sera collected from 12 HIV-infected donors at the time of seroconversion; and (2) nef serology in a population of 32 HIV seropositive and three seronegative hemophiliacs and their seronegative or seropositive sexual partners. The results obtained showed that anti-nef antibodies could not be detected in the sera tested independently of the appearance of antibodies specific to HIV structural proteins. Thus, the detection of anti-nef antibodies appears to be of little diagnostic value.
Laboratoire de Biochimie CNRS UA 1179, Faculté de Médecine, Marseille, France.
E
E
Bahraoui
A
A
Benjouad
J M
JM
Sabatier
J P
JP
Allain
Y
Y
Laurian
L
L
Montagnier
J C
JC
Gluckman
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000097:blood; D000939:Epitopes / Q000276:immunology; D005260:Female; D015693:Gene Products, nef / Q000276:immunology*; D015754:Genes, nef; D015483:HIV Antibodies / Q000032:analysis*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012699:Serology; D015229:Sexually Transmitted Diseases, Viral / Q000276:immunology; D054334:Viral Regulatory and Accessory Proteins / Q000276:immunology*; D054311:nef Gene Products, Human Immunodeficiency Virus
D015415:Biomarkers; D000939:Epitopes; D015693:Gene Products, nef; D015483:HIV Antibodies; D054334:Viral Regulatory and Accessory Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus