Amplification by the polymerase chain reaction of a specific target sequence in the gene coding for Escherichia coli verotoxin (VTe variant).
Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) protocol to target a specific sequence in the gene coding for the A subunit of Escherichia coli verotoxin (VTe-variant, VTev). This PCR protocol permits the VTe-variant target sequence to be distinguished from closely related sequences in the same coding regions for type 1, type 2, and type 2 variant E. coli verotoxins. This procedure will be a valuable adjunct to other DNA amplification techniques currently being used for molecular epidemiological studies of verotoxigenic E. coli.
National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.
W M
WM
Johnson
S D
SD
Tyler
G
G
Wang
H
H
Lior
D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D003854:Deoxyribonucleotides; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D003854:Deoxyribonucleotides; D022622:Shiga Toxin 1