Homology of mecA gene in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans to that of Staphylococcus aureus.
A penicillin-binding protein of molecular weight 76,000 inducible by beta-lactams was detected in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans. DNA from these strains hybridized to the mecA gene from Staphylococcus aureus; however, the chromosomal HindIII fragments containing the mecA genes were 3.4 kilobases in S. haemolyticus and 4.3 kilobases in S. simulans.
Ubukata
K
K
Department of Clinical Pathology, Teikyo University School of Medicine, Tokyo, Japan.
Nonoguchi
R
R
Song
M D
MD
Matsuhashi
M
M
Konno
M
M
D001426:Bacterial Proteins; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000032:analysis / Q000302:isolation & purification; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006602:Hexosyltransferases; D008712:Methicillin / Q000494:pharmacology*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000032:analysis / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010403:Penicillin Resistance; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D015183:Restriction Mapping; D013194:Staining and Labeling; D013210:Staphylococcus / Q000187:drug effects* / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D015247:Deoxyribonuclease HindIII; D002267:Muramoylpentapeptide Carboxypeptidase; D008712:Methicillin