Isolation and in vitro translation of mRNA from inflorescences of Parietaria judaica.
The aqueous extract of inflorescences of Parietaria judaica contains an allergen homologous to the major pollen allergen Par o I (14 kD), as shown by radio-allergosorbent test (RAST) inhibition and immunoblot analysis. Poly(A)+ RNA was obtained from inflorescences and was shown to be able to code in vitro for a protein homologous to Par o I with respect to sodium dodecylsulphate polyacrylamide gel electrophoretic mobility and to antigenic specificity as defined by the binding, in affinity chromatography, to solid-phase IgG of rabbit anti-Par o I antisera, and in RAST inhibition, to IgE antibodies of human reaginic serum pool.
Istituto Internazionale di Genetica e Biofisica, CNR, Napoli, Italia.
A
A
Scotto d'Abusco
F
F
Lo Schiavo
U
U
Oreste
M R
MR
Coscia
V
V
Santonastaso
G
G
Sacerdoti
A
A
Ruffilli
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000097:blood; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology; D066298:In Vitro Techniques; D011058:Pollen / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D011061:Poly A / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D011817:Rabbits; D011852:Radioallergosorbent Test
D000939:Epitopes; D012333:RNA, Messenger; D011061:Poly A; D007073:Immunoglobulin E; D012313:RNA