Human squamous cell carcinoma from skin: establishment and characterization of a new cell line (HSC-5).
A new cell line, designated as HSC-5 and derived from human skin squamous cell carcinoma (SCC), has been established in vitro and maintained proliferative in continuous tissue culture for over two years. The cells grow in a monolayer in vitro and have anaplastic epithelioid features. The doubling time was about 35 hr at the 30th passage. Chromosome analysis showed hypotetraploidy with a modal number of 76. A trial of transplantation of the cultured cells into nude mice was not successful. Analysis of cytokeratins from HSC-5 by two-dimensional gel electrophoresis revealed polypeptides No. 5, 8, 13, 18 and 19. The cell line is available to other investigators.
Department of Dermatology, Yamagata University School of Medicine, Japan.
Y
Y
Hozumi
S
S
Kondo
T
T
Shimoura
K
K
Aso
D002294:Carcinoma, Squamous Cell / Q000032:analysis / Q000235:genetics / Q000473:pathology*; D002460:Cell Line; D006801:Humans; D007633:Keratins / Q000032:analysis; D011123:Polyploidy; D012878:Skin Neoplasms / Q000032:analysis / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000473:pathology
D007633:Keratins