Characterization of HNK-1 bearing glycoproteins in human peripheral nerve myelin.
The HNK-1 carbohydrate epitope, which is shared by several members of the immunoglobulin gene super-family, is also the target epitope for IgM anti-MAG autoantibodies in patients with demyelinating neuropathy. By Western blot analysis, there are 7 HNK-1 immunoreactive glycoproteins in human peripheral nerve myelin, two of which have previously been identified as the myelin associated glycoprotein (MAG) and the P0 glycoprotein. In this study, the remaining HNK-1 bearing glycoprotein bands were characterized by immunoblot and NH2-terminal sequence analysis, and were all identified as degradation products or aggregates of the Po glycoprotein. MAG and P0 are therefore the only HNK-1 bearing glycoproteins in human peripheral nerve myelin.
Department of Neurology, Columbia University-College of Physicians and Surgeons, New York, NY 10032.
L H
LH
van den Berg
S A
SA
Sadiq
F P
FP
Thomas
N
N
Latov
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis*; D018959:CD57 Antigens; D002420:Cauda Equina / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008970:Molecular Weight; D009185:Myelin Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018959:CD57 Antigens; D006023:Glycoproteins; D009185:Myelin Proteins