The gene organisation of the human beta 2 integrin subunit (CD18).
We have studied the gene of the human beta 2 integrin subunit (CD18) and found it to be organised into 16 exons spanning a region of about 40 kb. All exon/intron boundaries conform to the GT/AG splicing consensus. The exons coding for the cysteine-rich region, which has been postulated to consist of 3 or 4 repeating elements, are not organised correspondingly. Transcription of the gene initiates from multiple sites which may be due to the absence of an upstream TATA box. The polyadenylation site is also heterogeneous. Five different sites were identified over a stretch of 10 bases.
Department of Biochemistry, University of Oxford, UK.
J B
JB
Weitzman
C E
CE
Wells
A H
AH
Wright
P A
PA
Clark
S K
SK
Law
D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D018821:CD18 Antigens; D002891:Chromosomes, Human, Pair 21; D005091:Exons; D015698:Genomic Library; D006801:Humans; D016023:Integrins / Q000235:genetics*; D007438:Introns; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014158:Transcription, Genetic
D015703:Antigens, CD; D018821:CD18 Antigens; D016023:Integrins; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides