A soluble form of the interleukin-1 receptor produced by a human B cell line.
A soluble protein that binds specifically to interleukin-1 (IL-1)beta was released from a B cell line (Raji). The covalently cross-linked binding protein/[125I]IL-1 beta migrated at 60 kDa by SDS-PAGE. The IL-1 receptor (IL-1R) on Raji cells had the same ligand specificity. Stimulation of Raji with dexamethasone increased surface expression of the IL-1R and the rate of release of soluble binding protein. A serine protease inhibitor prevented release of the binding protein and increased IL-1R expression on the cells. These results suggest that the soluble IL-1 beta binding protein is a proteolytically cleaved form of the novel B cell IL-1R.
University of Edinburgh, Department of Medicine, Northern General Hospital, UK.
J A
JA
Symons
G W
GW
Duff
D007611:Aprotinin / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D007700:Kinetics; D008985:Monensin / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000378:metabolism; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins / Q000378:metabolism
D007375:Interleukin-1; D011480:Protease Inhibitors; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D003609:Dactinomycin; D003907:Dexamethasone; D002738:Chloroquine; D008985:Monensin; D007611:Aprotinin; D003513:Cycloheximide