Immunocytochemical and ultrastructural characterization of endocrine cells in chicken proventriculus.
The endocrine cells of the chicken proventriculus were investigated immunocytochemically, using the peroxidase-antiperoxidase technique on paraffin and semithin sections for light microscopy, and immunogold staining in osmium-fixed material for electron microscopy. The fixation procedure also allowed a detailed ultrastructural investigation. Twenty-three antisera were tested and 7 immunoreactive cell-types were identified: D-cells containing somatostatin-like peptide; EG-cells immunoreactive to anti-glucagon, anti-GLP1 and anti-neurotensin; NT-cells labelled only with anti-neurotensin; BN-cells containing bombesin-like material; ENK-cells showing met-enkephalin immunoreactivity; EC-cells reactive to anti-serotonin; and APP-cells positive to anti-avian pancreatic polypeptide. In addition, enterochromaffin-like (ECL) cells, were also detected by electron microscopy. The presence of ENK-cells and the ultrastructure of these and NT-cells are described for the first time in chicken proventriculus, and glucagon. GLP1 and neurotensin are shown to be colocalized in the EG-cells.
Department of Histology and Pathology, University of Navarra, Pamplona, Spain.
A
A
Martínez
J
J
López
M A
MA
Barrenechea
P
P
Sesma
D000818:Animals; D001839:Bombesin / Q000378:metabolism; D002645:Chickens; D004744:Enkephalin, Methionine / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D006046:Gold; D007150:Immunohistochemistry / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods; D009496:Neurotensin / Q000378:metabolism; D009992:Osmium; D010191:Pancreatic Polypeptide / Q000378:metabolism; D011531:Proventriculus / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D012701:Serotonin / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism
D009992:Osmium; D012701:Serotonin; D009496:Neurotensin; D013004:Somatostatin; D004744:Enkephalin, Methionine; D010191:Pancreatic Polypeptide; D006046:Gold; D005934:Glucagon; D001839:Bombesin