Pseudorabies virus glycoprotein gI: in vitro and in vivo analysis of immunorelevant epitopes.
Overlapping fragments of the gene encoding glycoprotein gI of pseudorabies virus (PRV; herpesvirus suis 1) were expressed in bacteria. Using the fusion proteins and a panel of monoclonal antibodies (MAbs) against gI as well as swine sera we found that the N-terminal part of gI (residues 33 to approximately 100) contains a highly antigenic and immunogenic domain. Transfer of antibodies binding to this region as well as vaccination with fusion proteins containing the N terminus of gI are able to confer protection to mice against a lethal challenge of virus. The results show that gI, which is non-essential for virus replication in tissue culture, can induce neutralizing and protective antibodies. The potential suitability of fusion proteins encompassing N-terminal parts of gI as diagnostic tools is demonstrated.
Federal Research Centre for Virus Diseases of Animals, Tübingen, F.R.G.
W
W
Fuchs
H J
HJ
Rziha
N
N
Lukàcs
I
I
Braunschweiger
N
N
Visser
D
D
Lütticken
C S
CS
Schreurs
H J
HJ
Thiel
T C
TC
Mettenleiter
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D051379:Mice; D009500:Neutralization Tests; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013552:Swine; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins