Use of inorganic pyrophosphatase to improve the yield of in vitro transcription reactions catalyzed by T7 RNA polymerase.
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
P R
PR
Cunningham
J
J
Ofengand
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D008274:Magnesium / Q000494:pharmacology; D011755:Pyrophosphatases / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000096:biosynthesis; D012336:RNA, Ribosomal, 16S / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects*; D014764:Viral Proteins
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D011755:Pyrophosphatases; D008274:Magnesium