The effect of heparin on fibronectin and thrombospondin synthesis and mRNA levels in cultured human endothelial cells.
Studies to eludicate the effect of heparin on the synthesis of extracellular matrix components by cultured human umbilical vein endothelial cells (EC) were conducted. Using pulse-labeling and ELISA techniques, we found that EC grown in the presence of heparin (90 micrograms/ml) and endothelial cell growth factor (ECGF) synthesized 50% less fibronectin (FN) than did ECGF-treated control cultures. No change in the synthesis of thrombospondin (TSP) was induced by heparin. The effect of heparin on EC FN synthesis was independent of whether the cells were cultivated on plastic or gelatin substrates. However, ECGF modulates the effect of heparin on EC synthesis of FN. RNA slot-blot analysis demonstrated that heparin treatment specifically decreased the steady-state mRNA levels for both FN and TSP in the cells. Steady-state levels of mRNA for two intracellular proteins, actin and tubulin, were unchanged. These data suggest that heparin decreases EC expression of FN at least in part by decreasing the amount of FN mRNA available for translation. The failure of heparin to inhibit TSP expression, although it reduces TSP mRNA levels, points to the possibility that the rate of EC synthesis of TSP is translationally or post-translationally regulated.
Connective Tissue Research Institute, University of Pennsylvania, Philadelphia 19104.
B
B
Lyons-Giordano
J M
JM
Brinker
N A
NA
Kefalides
D002478:Cells, Cultured; D016228:Endothelial Growth Factors; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D006133:Growth Substances / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D019699:Thrombospondins
D016228:Endothelial Growth Factors; D005353:Fibronectins; D006133:Growth Substances; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D019699:Thrombospondins; D006493:Heparin