Transcription of the heat shock 70 locus in Trypanosoma brucei.
The 23-kb heat shock 70 locus of the protozoan parasite Trypanosoma brucei encodes several tightly clustered genes. From 5' to 3', a cognate hsp70 gene (gene 1) (see preceding paper, Lee et al.) is separated from a cluster of five identical hsp70 genes (genes 2-6) by about 6 kb of DNA that encodes several RNAs of unknown function. Polycistronic transcripts could be detected in the tandem array of hsp70 genes 2-6 and the maturation of the hsp70 pre-mRNAs involves addition of mini-exons on at least two different alternate 3' splice acceptor sites. Potential heat shock transcription factor binding sites are present upstream of hsp70 gene 2 and in the intergenic regions of hsp70 genes 2-6, but could not be found upstream of the cognate hsp70 gene 1.
Department of Genetics and Development, Columbia University, New York, NY 10032.
M G
MG
Lee
L H
LH
Van der Ploeg
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D005091:Exons; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D012326:RNA Splicing; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics*
D006360:Heat-Shock Proteins; D012313:RNA