[The histochemical detection of insulin in a culture of pancreatic endocrine tissue by using the pseudoisocyanine and immunofluorescence methods].
Histochemical pseudoisocyanin and immunofluorescent methods were employed to investigate the level of insulin in cultivated pancreatic islet tissue (PIT) using two techniques: 1) staining of isolated PIT, cultivated on mica sheets; 2) staining of cultivated PIT sections. Stained sections ensure better results of microscopic and photometric investigations. On the whole, the most optimum method for histochemical analysis of the state of cultivated PIT is the combination of the above two techniques.
Meĭramov
G G
GG
Kohnert
K D
KD
Turchin
I S
IS
Tusupbekova
G T
GT
Akhmetov
A A
AA
Meĭramova
Zh G
ZhG
D000818:Animals; D000831:Animals, Newborn; D046508:Culture Techniques / Q000379:methods; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry / Q000379:methods; D007328:Insulin / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism*; D011804:Quinolines; D013194:Staining and Labeling / Q000379:methods; D013552:Swine
D007328:Insulin; D011804:Quinolines; C100254:pseudoisocyanine
Gistokhimicheskoe vyiavlenie insulina v kul'ture éndokrinnoĭ tkani podzheludochnoĭ zhelezy s pomoshch'iu psevdoizotsianinovogo i immunofliuorestsentnogo metodov.