Thy 1+ donor cells that promote allograft tolerance in sublethally irradiated MHC-disparate hosts.
We have previously demonstrated that depletion of Thy 1+ cells impairs the capacity of C57BL/6J, H-2b bone marrow cells, BMC, and BALB/cxC57BL/6J F1 BMC and spleen cells (SC) to establish mixed lymphoid chimerism and tolerance for donor-specific skin grafts in sublethally irradiated (240 cGy x3) BALB/c, H-2d hosts. In the present studies incubation with anti Ly2.2 + C markedly reduced the capacity of BALB/cxC57BL/6J, F1 SC to induce tolerance and chimerism (P less than .001). Incubation with anti-L3T4 + C had an inhibitory effect of borderline significance (P less than .04). Incubation with L-leucyl-L-leucine methyl ester (which removes NK cells, Tc, and precursor Tc) had no effect on the capacity of either C57BL/6J BMC or BALB/cxC57BL/6J F1 BMC or SC to establish chimerism and induce skin graft tolerance. These results suggest that tolerance-promoting Thy 1+ cells are not cytotoxic T cells. Both Ly2+ noncytotoxic CD8+ and L3T4+ noncytotoxic CD4+ cells may be involved. Alternatively the requisite Thy 1+ cells may be immature T cells that express both Ly2 and L3T4.
University of Kansas Medical Center, Kansas City 66103.
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D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D016026:Bone Marrow Transplantation; D004151:Dipeptides / Q000494:pharmacology; D006183:H-2 Antigens / Q000276:immunology*; D006648:Histocompatibility; D007108:Immune Tolerance; D051379:Mice; D008815:Mice, Inbred Strains; D016038:Skin Transplantation; D013601:T-Lymphocytes / Q000502:physiology*; D018800:Thy-1 Antigens; D014184:Transplantation, Homologous; D014916:Whole-Body Irradiation
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D004151:Dipeptides; D006183:H-2 Antigens; D018800:Thy-1 Antigens; C045139:leucyl-leucine-methyl ester