Comparative mapping of ZFY in the hominoid apes.
Within our project of comparative mapping of candidate genes for sex-determination/testis differentiation, we used a cloned probe from the human ZFY locus for comparative hybridization studies in hominoids. As in the human, the ZFY probe detects X- and Y-specific restriction fragments in the chimpanzee, the gorilla, the orangutan, and the gibbon. Furthermore, the X-specific hybridization site in the great apes resides in Xp21.3, the same locus defining ZFX in the human. The Y-specific locus of ZFY maps closely to the early replicating pseudoautosomal segment in the telomeric or subtelomeric position of the Y chromosomes of the great apes, again as found in the human. Thus, despite cytogenetically visible structural alterations within the euchromatic parts of the Y chromosomes of the human species and the great apes, a segment of the Y chromosome defined by the pseudoautosomal region and ZFY seems to be more strongly conserved than the rest of the Y chromosome.
Institut für Humangenetik und Anthropologie der Universität, Freiburg, Federal Republic of Germany.
G
G
Müller
W
W
Schempp
D000818:Animals; D005075:Biological Evolution; D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006922:Hylobates / Q000235:genetics*; D051741:Kruppel-Like Transcription Factors; D008297:Male; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012732:Sex Determination Analysis; D014157:Transcription Factors; D014960:X Chromosome; D014998:Y Chromosome
D015342:DNA Probes; D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors; C054430:ZFY protein, human