[A study on expression of various oncogenes and tumor-associated genes in LEC rats spontaneously developing hepatitis and hepatoma].
The expression of nine proto-oncogenes (c-myc, N-myc, c-fos, c-jun, p53, H-ras, N-ras, c-raf, hst) and other three genes (AFP, PCNA, GST-P) were investigated during spontaneous development to hepatocellular carcinomas (HCCs) in LEC rats. Expressions of c-myc, H-ras, N-ras, c-raf, p53, and PCNA genes were detected but did not significantly change during the development to HCCs in LEC rats. Expressions of N-myc, hst, and AFP genes were not detectable since 5 weeks after birth. Expression of c-fos gene was detected in one out of four HCCs. Significantly increased expression of c-jun gene was observed in the liver tissues of LEC rats aged 8 months. The high expression was decreased in HCCs. On the other hand, the expression of GST-P gene increased in parallel with the clinical course of the development to HCCs in LEC rats. The increased expression of GST-P gene was observed in the liver tissues of LEC rats aged 8 months, and HCCs showed very high expression of GST-P gene. These observations suggest that both c-jun and GST-P genes may play a role in the spontaneous development to HCCs in LEC rats.
First Department of Surgery, Hokkaido University, School of Medicine, Sapporo, Japan.
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Suzuki
D000818:Animals; D015152:Blotting, Northern; D015870:Gene Expression; D011905:Genes, ras; D005982:Glutathione Transferase / Q000235:genetics*; D006520:Hepatitis, Animal / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics*; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D016759:Oncogene Protein p65(gag-jun); D018809:Proliferating Cell Nuclear Antigen; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D015682:Retroviridae Proteins, Oncogenic; D000509:alpha-Fetoproteins / Q000235:genetics*
D009687:Nuclear Proteins; D016759:Oncogene Protein p65(gag-jun); D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D015682:Retroviridae Proteins, Oncogenic; D000509:alpha-Fetoproteins; D005982:Glutathione Transferase