Ordering three DNA polymorphisms on human chromosome 3 by sperm typing.
Three loci on the short arm of human chromosome 3 were ordered by sperm typing to expand the limited genetic map of this region. Almost 300 individual sperm from a donor triply heterozygous at D3S2, D3S11, and D3S12 were amplified by PCR using primers flanking the polymorphic site at each locus. Primary PCR product was reamplified using allele-specific primers of different lengths, allowing the allelic state at each locus to be determined by gel electrophoresis. Maximum likelihood analysis of the sperm-typing data showed that the most likely order was D3S2-D3S11-D3S12 with an odds ratio of almost 5000:1 when compared to the next most likely order. This finding should be useful in interpreting loss of heterozygosity on 3p in a variety of cancers. Our results also demonstrate the practicality of ordering DNA polymorphisms using sperm typing.
Department of Biomathematics, University of California, Los Angeles 90032.
T M
TM
Goradia
V P
VP
Stanton
X F
XF
Cui
H
H
Aburatani
H H
HH
Li
K
K
Lange
D E
DE
Housman
N
N
Arnheim
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D002893:Chromosomes, Human, Pair 3; D004247:DNA / Q000235:genetics*; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013094:Spermatozoa / Q000737:chemistry*
D005819:Genetic Markers; D004247:DNA