Four epitopes of Pseudomonas aeruginosa elastase defined by monoclonal antibodies.
Monoclonal antibodies against the elastase of Pseudomonas aeruginosa were produced from spleen cells of BALB/c mice primed with purified elastase of P. aeruginosa and P3-X63-Ag8-U1 myeloma cells. The six clones established generated antibodies which reacted with a 33,000-Da peptide and recognized four different elastase epitopes by a competitive binding enzyme-linked immunosorbent assay. The monoclonal antibodies designated as ELA-17 and ELA-42 that recognize two different epitopes reacted by dot-enzyme immunoassay and by Western immunoblotting with all clinical and International Antigen Typing Scheme strains of P. aeruginosa positive for elastase.
Department of Microbiology and Immunology, Faculty of Medicine, University of Montreal, Quebec, Canada.
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J
Lagacé
M
M
Fréchette
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000939:Epitopes / Q000032:analysis*; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D010196:Pancreatic Elastase / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010196:Pancreatic Elastase