Ischemia reperfusion injury and histamine release in isolated and perfused guinea-pig heart: pharmacological interventions.
Experiments were carried out to provide further information on the biochemical and morphological changes occurring in the guinea-pig heart after multiple ligature and reopening of the left anterior descending (LAD) coronary artery. In isolated perfused guinea-pig heart the reopening of LAD coronary artery leads to a release of histamine related to a loss of metachromasia by cardiac mast cells. The process is associated with malonyldialdehyde (MDA) production, cellular overload of calcium and ventricular arrhythmias which can be modulated by pharmacological interventions.
Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.
E
E
Masini
S
S
Bianchi
F
F
Gambassi
B
B
Palmerani
A
A
Pistelli
L
L
Carlomagno
P F
PF
Mannaioni
D000818:Animals; D003331:Coronary Vessels / Q000502:physiology; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects / Q000503:physiopathology*; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*; D009206:Myocardium / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*
D007770:L-Lactate Dehydrogenase