Synthesis of lysyl oxidase in experimental hepatic fibrosis.
The synthesis of lysyl oxidase, which initiates the cross-linking of collagen and elastin, was investigated in carbon tetrachloride (CCl4) induced fibrotic liver of rat. Lysyl oxidase activity of the fibrotic liver was 4 times greater than that of normal liver. mRNAs from the livers of normal and CCl4-treated rats were prepared for in vitro protein synthesis and the products were analyzed by immunoprecipitation with a monoclonal antibody against lysyl oxidase. The mRNAs from the fibrotic liver gave more than 3 times higher level of messenger copies for lysyl oxidase than did mRNAs from normal liver. The molecular weight of the nascent lysyl oxidase was 48,000.
Wakasaki
H
H
Department of Pathology, Wakayama Medical College, Japan.
Ooshima
A
A
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000201:enzymology; D004195:Disease Models, Animal; D005260:Female; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008103:Liver Cirrhosis / Q000201:enzymology*; D014176:Protein Biosynthesis; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D012313:RNA; D000594:Amino Acid Oxidoreductases; D008249:Protein-Lysine 6-Oxidase