Transcriptional regulation of rat liver protein disulphide-isomerase gene by insulin and in diabetes.
The mRNA encoding for rat protein disulphide-isomerase (PDI) increases 3-fold in the liver of diabetic rats and is accompanied by similar changes at the protein level. Long treatment (for 3 days) of diabetic rats with insulin reverses this effect of diabetes both at the mRNA and protein levels. The higher expression of rat PDI mRNA in diabetes is due to an increase in the transcriptional rate of the gene, and insulin treatment of diabetic animals produces within 30 min a decrease in the level of transcription of PDI gene, as judged by nuclear run-on transcription experiments performed in vivo. These results clearly show a role for insulin in the regulation of transcription of the gene encoding this multifunctional protein in rat liver.
Departamento de Bioquímica, Facultad de Medicina de la UAM, Madrid, Spain.
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects; D015723:Gene Library; D005796:Genes / Q000187:drug effects; D006801:Humans; D007328:Insulin / Q000494:pharmacology* / Q000627:therapeutic use; D007535:Isomerases / Q000235:genetics* / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019704:Protein Disulfide-Isomerases; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000187:drug effects
D007328:Insulin; D012333:RNA, Messenger; D012313:RNA; D007535:Isomerases; D019704:Protein Disulfide-Isomerases