Z-DNA affinity chromatography.
In this chapter we have detailed a method that can be generalized to link virtually any DNA substrate to a chromatography matrix at its ends via an avidin-biotin linkage. We have used this technique to construct a left-handed Z-DNA column for the purpose of identification and purification of Z-DNA-binding proteins. This technique for the linkage of DNA to a column matrix by avidin-biotin technology can be modified, however, to produce linked multimeric sequences specific for regulatory or other DNA-binding proteins.
Fishel
R
R
Anziano
P
P
Rich
A
A
D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001710:Biotin; D002846:Chromatography, Affinity / Q000379:methods; D002942:Circular Dichroism; D004247:DNA; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D007202:Indicators and Reagents; D008837:Micrococcus / Q000201:enzymology; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D011089:Polydeoxyribonucleotides; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet; D013604:T-Phages / Q000201:enzymology
D007202:Indicators and Reagents; D011089:Polydeoxyribonucleotides; C019530:poly(dC-dG); D001710:Biotin; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase