The promoter of the proliferating cell nuclear antigen (PCNA) gene is active in serum-derived cells.
mRNA levels for the Proliferating Cell Nuclear Antigen (PCNA) gene are growth regulated. PCNA promoters of different lengths were used to drive a linked reporter, the cDNA for human thymidine kinase (TK). After transfection into TK ts13 cells, stable cell lines were obtained. Regardless of promoter length, in all cell lines the levels of TK mRNA were roughly similar in serum-deprived and serum stimulated cells, confirming, by an independent method, that the growth regulation of PCNA mRNA levels doe not depend on the 5' flanking sequence of the PCNA gene.
Department of Pathology, Temple University Medical School, Philadelphia, PA 19140.
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Ottavio
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Chang
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Rizzo
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Petralia
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Travali
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Baserga
D000818:Animals; D001324:Autoantigens / Q000235:genetics; D002460:Cell Line; D003470:Culture Media; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D010957:Plasmids; D018809:Proliferating Cell Nuclear Antigen; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014162:Transfection
D001324:Autoantigens; D003470:Culture Media; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D012313:RNA