[Effect of intracellular administration of L-tyrosine and L-phenylalanine on voltage dependent calcium current in PC 12 pheochromocytoma cells].
Changes in the high-threshold calcium current (Ica) induced by intracellular administration of L-tyrosine and L-phenylalanine have been studied on internally perfused PC 12 pheochromocytoma cells using whole-cell voltage-clamp technique. L-tyrosine (20 microM/l) not only prevented a decay of Ica occurring during intracellular perfusion but induced also its transient recovery. In contrast, L-phenylalanine (20 microM/l) accelerated a decline of Ica. Replacement of ATP in the perfusing solution by an equivalent amount of ADP (2 mmol/l) did not alter the effect of amino acids. alpha-methyl-D, L-tyrosine (a specific blocker of tyrosine hydroxylase) caused the effect similar to that of L-tyrosine.
A E
AE
Martyniuk
N Kh
NKh
Pogorelaia
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000310:Adrenal Gland Neoplasms / Q000503:physiopathology*; D000818:Animals; D015220:Calcium Channels / Q000187:drug effects*; D008781:Methyltyrosines / Q000494:pharmacology; D010649:Phenylalanine / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000503:physiopathology*; D051381:Rats; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000494:pharmacology*; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors; D019805:alpha-Methyltyrosine
D015220:Calcium Channels; D008781:Methyltyrosines; D014443:Tyrosine; D010649:Phenylalanine; D000244:Adenosine Diphosphate; D019805:alpha-Methyltyrosine; D014446:Tyrosine 3-Monooxygenase
Vliianie vnutrikletochnogo vvedeniia L-tirozina i L-fenilalanina na potentsialozavisimyĭ kal'tsievyĭ tok v kletkakh feokhromotsitomy PC 12.