The effect of neurotoxin on rabies virus binding to mouse neuroblastoma cells.
Mouse neuroblastoma cells were exposed to alpha bungarotoxin, a neurotoxin known to inhibit rabies virus binding to the nicotinic acetylcholine receptor located at the neuromuscular junction in muscle tissue. The total amount of 3H-CVS virus that bound to neurotoxin treated cells was separated into specific and non-specific binding using a cold competition assay. Comparison of untreated and neurotoxin treated cells demonstrated that the majority of cell-associated virus in untreated cells was of a specific nature whereas the majority of the cell-associated virus in neurotoxin treated cells was due to non-specific binding.
Department of Veterinary Diagnosis, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
D J
DJ
Briggs
R M
RM
Phillips
D000818:Animals; D001667:Binding, Competitive; D002038:Bungarotoxins / Q000494:pharmacology*; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D011820:Rabies virus / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000187:drug effects* / Q000378:metabolism; D011991:Receptors, Virus / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D002038:Bungarotoxins; D011978:Receptors, Nicotinic; D011991:Receptors, Virus