Immunocytochemical characterization of immune cells in lesions of American cutaneous leishmaniasis using novel T cell markers.
Some recently defined lymphocyte immunophenotypes were determined in lesions of patients with American cutaneous leishmaniasis (ACL). New monoclonal antibodies have allowed the demonstration of cell surface antigens of T lymphocytes, such as CD45RA and CD45RO, which recognize different maturational stages of the same T CD4+ cell subgroup: 'virgin' (CD4+CD45RA+) and 'memory' (CD4+CD45RO+) T cells respectively. The CD4/CD8 cell ratios were higher in mucocutaneous leishmaniasis (MCL) than in localized cutaneous leishmaniasis (LCL) lesions. Diffuse cutaneous leishmaniasis (DCL) has the highest values of 'virgin' T cells; LCL and MCL patients have lower values, similar to each other. 'Memory' T cells were higher in MCL than in LCL or DCL. The ratio of 'memory'/'virgin' T cells was 7.9 for LCL, 9.6 for MCL and 2.5 for DCL. The highest value for IL-2 receptor positive cells (CD25) was observed in LCL, whereas single CD45RO-immunoreactive cells showed a peak value in DCL patients. HLA-DR+ cells were present in all three clinical forms of ACL. MCL patients showed a lack of epithelial Langerhans cell (CD1a+) in the nasal mucosa.
Martínez-Arends
A
A
Instituto de Biomedicina, Universidad Central de Venezuela, Caracas.
Tapia
F J
FJ
Cáceres-Dittmar
G
G
Mosca
W
W
Valecillos
L
L
Convit
J
J
D015703:Antigens, CD / Q000032:analysis*; D015415:Biomarkers; D001706:Biopsy; D016516:CD4-CD8 Ratio; D002452:Cell Count; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000473:pathology; D012867:Skin / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; D015415:Biomarkers