Requirement of MHC class I antigen expression on human tumors for in vitro recognition by autologous blood lymphocytes.
Expression of class I antigens on ex-vivo tumor cells was found to be required for their recognition by autologous blood lymphocytes in vitro. Lymphocyte stimulation (auto-tumor stimulation ATS) in mixed lymphocyte tumor cell cultures (MLTC) and cytolysis in short term assays (autologous lymphocyte-mediated cytotoxicity ALC) were positive with cells of a proportion of sarcoma and carcinoma patients. In addition to the class I antigens, these tumors also carried the adhesion molecule ICAM-1. Monoclonal antibodies (MAb) to the MHC class I, but not to class II antigens inhibited lymphocyte proliferation in the MLTC and lysis of tumor cells in the ALC tests. In a proportion of originally negative or low expressor tumors, in vitro treatment with Interferon gamma and tumor necrosis factor alpha induced the expression of class I antigens and/or ICAM-1. In the MLTC the cytokine treated tumor cells stimulated the blood lymphocytes and generated cytotoxic effectors which reacted also with the untreated tumor cells.
Vánky
F
F
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Klein
E
E
D015815:Cell Adhesion Molecules / Q000502:physiology; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D000949:Histocompatibility Antigens Class II / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D009369:Neoplasms / Q000188:drug therapy / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015815:Cell Adhesion Molecules; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma