Galanin-like immunoreactivity is influenced by estrogen in peripubertal and adult rats.
Galanin gene expression in the anterior pituitary is potently stimulated by estrogen in adult rats. To evaluate the influence of estrogen on galanin during the peripubertal period 30- to 32-day-old female rats were treated with pregnant mare serum gonadotropin (PMSG, 10 IU s.c., 10.00 h). Galanin-like immunoreactivity (galanin-LI) in hypothalamic and pituitary tissues was evaluated 1, 2 or 3 days after PMSG treatment between 17.00 and 19.00 h. The PMSG treatment stimulated 17 beta-estradiol secretion, which induced a midafternoon LH surge 2 days after the PMSG treatment. Concentrations of galanin-LI at the time of this LH surge were elevated 82% in the anterior pituitary and 58% in the hypothalamus (without the median eminence) when compared to saline-treated female rats. On the 3rd day after the PMSG injection, galanin-LI was increased 236% in the anterior pituitary, 88% in the neurointermediate lobe and 39% in the median eminence compared to saline-treated female rats. These changes in galanin-LI were not observed in similarly aged male rats or ovariectomized rats treated with PMSG. In adult male rats, daily injections with 17 beta-estradiol valerate (10 micrograms/daily s.c.) for 1 week increased galanin-LI in the median eminence and neurointermediate lobe to an extent similar to that seen in juvenile female rats following PMSG treatment. In contrast, the high serum levels of 17 beta-estradiol achieved after 17 beta-estradiol valerate treatment increased galanin-LI in the anterior pituitary 65-fold. These studies indicate that galanin-LI is influenced by estrogen in peripubertal and adult rats.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Psychiatry, Mount Sinai School of Medicine, New York, N.Y.
S M
SM
Gabriel
J I
JI
Koenig
L M
LM
Kaplan
D000818:Animals; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D019004:Galanin; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D007031:Hypothalamus / Q000032:analysis* / Q000187:drug effects; D008297:Male; D008473:Median Eminence / Q000032:analysis* / Q000187:drug effects; D009479:Neuropeptides / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D010902:Pituitary Gland / Q000032:analysis* / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012741:Sexual Maturation / Q000187:drug effects* / Q000502:physiology
D006064:Gonadotropins, Equine; D009479:Neuropeptides; D010455:Peptides; D004958:Estradiol; D019004:Galanin