An immunochemical analysis of precipitating and non-precipitating idiotype-anti-idiotype reactions.
Two monoclonal IgG3 syngeneic anti-idiotypes are described which form soluble and insoluble complexes with anti-alpha(1----6)dextran hybridoma and myeloma proteins. Specific precipitation was seen when purified anti-alpha(1----6)dextrans were added to ascitic fluid containing IgG3 kappa anti-idiotype. Analysis of the supernatants of the idiotype-anti-idiotype precipitates demonstrated the presence of soluble complexes whose mobilities in polyacrylamide gels could, in some cases, be distinguished from that of free anti-idiotype. An IgG1 kappa anti-idiotype is described which did not form precipitates with anti-alpha(1----6)dextrans unless 3% PEG 6000 was added.
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
P
P
Borden
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EA
Kabat
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D003911:Dextrans / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D066298:In Vitro Techniques; D051379:Mice; D011092:Polyethylene Glycols; D011233:Precipitin Tests; D012995:Solubility
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D003911:Dextrans; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D011092:Polyethylene Glycols