Identification of a somatostatin-14-generating propeptide converting enzyme as a member of the kex2/furin/PC family.
A propeptide converting enzyme capable of producing somatostatin-14 has been identified and partially characterized from anglerfish pancreatic islets. Results from N-terminal protein sequence analysis indicate that this enzyme is a member of the kex2/furin/PC family. This observation provides strong corroborative evidence that the kex2/furin/PC protease family is involved in propeptide conversion. Comparison of the obtained protein sequence with the cDNA sequence of mammalian PC2 also suggests that the active enzyme is derived from a precursor by cleavage at a site containing four consecutive basic amino acids.
Dept. of Molecular Neuroendocrinology, Max Planck Institute for Experimental Medicine, Göttingen, Germany.
R B
RB
Mackin
B D
BD
Noe
J
J
Spiess
D000595:Amino Acid Sequence; D000818:Animals; D005399:Fishes; D045683:Furin; D006863:Hydrogen-Ion Concentration; D007515:Islets of Langerhans / Q000201:enzymology*; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D043484:Proprotein Convertases; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D013004:Somatostatin / Q000096:biosynthesis*; D013381:Subtilisins / Q000378:metabolism
D029701:Saccharomyces cerevisiae Proteins; C071112:somatostatin-14 propeptide converting enzyme; D013004:Somatostatin; D043484:Proprotein Convertases; D012697:Serine Endopeptidases; D013381:Subtilisins; C058751:KEX2 protein, S cerevisiae; D045683:Furin