Monoclonal antibody to brain cytoplasmic tetrodotoxin-sensitive protein detects an epitope associated with lymphocytes and involved in lymphocyte activation.
We have previously derived a monoclonal antibody, BIP-4, which is specific to a mammalian brain protein representing a type of sodium channel. Here we show that this antibody detects an epitope associated with lymphocytes and that it triggers a proliferative response of the cells. BIP-4 epitope can be detected on both human peripheral blood and murine splenic mononuclear cells. Surface immuno-globulin-negative (i.e. resting T) lymphocytes are neither bound by the antibody nor proliferate to it. Proliferative response exerted in 7-day cultures of murine splenic mononuclear cells by recombinant interleukin-2 was blocked by BIP-4 antibody. We conclude that the epitope shared by a type of brain sodium channel protein and lymphocyte surface is involved in some, yet unrecognized, step of immune cell activation.
Pinchuk
L N
LN
Department of Neurochemistry, A.A. Bogomoletz Institute of Physiology, Academy of Sciences of the Ukrainian S.S.R., Kiev.
Pinchuk
G V
GV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000502:physiology; D001921:Brain / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D000939:Epitopes; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D011994:Recombinant Proteins; D013779:Tetrodotoxin / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007376:Interleukin-2; D009419:Nerve Tissue Proteins; D011947:Receptors, Antigen, B-Cell; D011994:Recombinant Proteins; D013779:Tetrodotoxin