On multiple-nutrient-limited growth of microorganisms, with special reference to dual limitation by carbon and nitrogen substrates.
Simultaneous limitation of microbial growth by two or more nutrients is discussed for dual carbon/nitrogen-limited growth in continuous culture. The boundaries of the zone where double-limited growth occurs can be clearly defined from both cultivation data and cellular composition and they can be also predicted from growth yield data measured under single-substrate-limited conditions. It is demonstrated that for the two nutrients carbon and nitrogen the zone of double nutrient limitation is dependent on both the C:N ratio of the growth medium and the growth (dilution) rate. The concept on double-(carbon/nitrogen)-limited growth presented here can be extended to other binary and multiple combinations of nutrients.
Swiss Federal Institute for Water Resources and Water Pollution Control (EAWAG), Dübendorf.
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D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D002175:Candida / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005970:Glutamate Synthase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*
D003470:Culture Media; D002244:Carbon; D005970:Glutamate Synthase; D005969:Glutamate Dehydrogenase; D005974:Glutamate-Ammonia Ligase; D008274:Magnesium; D009584:Nitrogen