Interaction of substance P, compound 48/80 and mastoparan with the alpha-subunit C-terminus of G protein.
Pretreatment of purified calf brain G proteins with activated pertussis toxin or antibodies raised against the C-terminus of their alpha subunits prevented the increase in GTPase activity induced by substance P, compound 48/80 and mastoparan. These results suggest that these mast cell secretagogues activate G proteins directly via an interaction with the C-terminus of alpha subunits of G proteins by mimicking the agonist-liganded receptors.
Laboratoire de Neuroimmunopharmacologie, Université Louis Pasteur, Strasbourg I, France.
M
M
Mousli
C
C
Bronner
J
J
Bockaert
B
B
Rouot
Y
Y
Landry
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002417:Cattle; D004789:Enzyme Activation / Q000187:drug effects; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D010455:Peptides; D037342:Pertussis Toxin; D011485:Protein Binding; D013373:Substance P / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D014862:Wasp Venoms / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D000906:Antibodies; D036341:Intercellular Signaling Peptides and Proteins; D010446:Peptide Fragments; D010455:Peptides; D010566:Virulence Factors, Bordetella; D014862:Wasp Venoms; D013373:Substance P; D003189:p-Methoxy-N-methylphenethylamine; C037500:mastoparan; D037342:Pertussis Toxin; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins