The ionic response of the liver fluke, Fasciola hepatica to ouabain, monensin and the deacetylated (amine) metabolite of diamphenethide.
1. The ionic response of the liver fluke, Fasciola hepatica to perturbation of Na,K-pump activity has been determined by atomic absorption spectrophotometry. 2. The Na+/K(+)-ATPase inhibitor ouabain (1 x 10(-4) M) induced a marked reduction in K+ levels; Na+ and Ca2+ levels also fell. 3. The sodium ionophore monensin (1 x 10(-4) M) also caused a decrease in K+ levels, to below that of Na+. 4. Brief pretreatment with ouabain (1 x 10(-4) M, 15 min) followed by monensin treatment did not affect the decline in K+ levels, but did prevent the short-lived Na+ decline observed with monensin alone. 5. The deacetylated (amine) metabolite of the fasciolicide diamphenethide caused a short-lived drop in Na+ levels, but otherwise produced little change in ion levels within the fluke.
School of Biology and Biochemistry, Queen's University of Belfast, U.K.
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Caseby
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Fairweather
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Harriott
D000215:Acylation; D000588:Amines / Q000378:metabolism; D000818:Animals; D003957:Diamfenetide / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005210:Fasciola hepatica / Q000378:metabolism*; D008985:Monensin / Q000494:pharmacology*; D010042:Ouabain / Q000494:pharmacology*
D000588:Amines; D010042:Ouabain; D008985:Monensin; D003957:Diamfenetide; D004121:Dimethyl Sulfoxide