Antibodies to the beta-amyloid peptide cross-react with conformational epitopes in human fibrinogen subunits from peripheral blood.
Antibodies to the Alzheimer disease (AD) beta-amyloid peptide (beta AP) were used to identify beta AP precursor fragments in blood. The antibodies detected 3 major polypeptides with apparent molecular weights (MW) of 47-64,000 in Western blots of plasma derived clot proteins, but these proteins corresponded to human A-alpha, B-beta and gamma-fibrinogen since they reacted with 2 different anti-fibrinogen antisera, and the anti-beta AP and anti-fibrinogen antibodies recognized purified fibrinogen and fibrin. These data are significant for efforts to develop immunochemical assays to diagnose and monitor the progression of AD.
Stern
R A
RA
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19104.
Trojanowski
J Q
JQ
Lee
V M
VM
D000310:Adrenal Gland Neoplasms / Q000032:analysis; D000544:Alzheimer Disease / Q000378:metabolism; D000682:Amyloid / Q000032:analysis / Q000276:immunology*; D016229:Amyloid beta-Peptides; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D001923:Brain Chemistry; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D005340:Fibrinogen / Q000276:immunology*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000276:immunology*; D010673:Pheochromocytoma / Q000032:analysis; D011487:Protein Conformation; D051381:Rats
D000682:Amyloid; D016229:Amyloid beta-Peptides; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes; D009419:Nerve Tissue Proteins; D005340:Fibrinogen