Repetitive nucleotide sequencing of a dispensable DNA segment in a clonal population of African swine fever virus.
Repetitive nucleotide sequencing of a dispensable genomic segment of a clonal population of African swine fever (ASF) virus has been carried out to estimate the mutant frequency to neutral alleles. Since no mutations have been detected in a total of 54026 nucleotides screened, the maximum mutant frequency is 5.5 x 10(-5) substitutions/nucleotide (95% confidence level). The result renders very unlikely the occurrence of hypermutational events during ASF virus DNA replication, at least within the selected DNA fragment.
Yáñez
R J
RJ
Centro de Biología Molecular (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Cantoblanco, Madrid, Spain.
Moya
A
A
Viñuela
E
E
Domingo
E
E
D000358:African Swine Fever Virus / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D016366:Open Reading Frames; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004279:DNA, Viral