Microinjections of norepinephrine into the intermediolateral cell column of the spinal cord exert excitatory as well as inhibitory effects on the cardiac function.
Cardiac responses to microinjections of norepinephrine (NE) into the intermediolateral column of the spinal cord (IML) at T2 level were studied in pentobarbital-anesthetized, immobilized and artificially ventilated, male Wistar rats. For describing the effects of NE conveniently, the doses of NE were divided into two ranges. The small dose-range consisted of 20 nl volumes of 50, 75 and 100 micromolar (microM) solutions (i.e. 1, 1.5 and 2 pmole in 20 nl, respectively). The larger dose-range consisted of 20 nl volumes of 2.5, 25, 40 and 50 millimolar (mM) solutions (i.e. 0.05, 0.5, 0.8 and 1 nmole in 20 nl, respectively). Injections of small doses of NE (1-2 pmole) into the IML increased heart rate (HR); intravenous injections of these doses did not alter either blood pressure (BP) or HR. Larger doses of NE (0.05-1 nmole) elicited a decrease in HR; intravenous injections of these doses increased HR and BP. Maximum increase in HR was produced by injections of 1.5 pmole of NE into the IML; this effect was blocked by prior injections of prazosin (an alpha 1 adrenergic receptor antagonist; 50 pmole) but not idazoxan (an alpha 2 adrenergic receptor blocker; 10 pmole) into the IML. Maximum decrease in HR was elicited by injections of 0.8 nmole of NE into the IML; this effect was blocked by idazoxan (10 pmole) but not prazosin (50 pmole). Microinjections of idazoxan (10 pmole) alone increased HR while prazosin (50 pmole) alone was ineffective. Intravenous injections of chlorisondamine (a ganglion blocker) blocked the increase in HR elicited by injections of 1.5 pmole of NE into the IML.(ABSTRACT TRUNCATED AT 250 WORDS)
Section of Neurosurgery, University of Medicine and Dentistry of New Jersey Medical School, Newark 07103.
K
K
Sundaram
J
J
Murugaian
H
H
Sapru
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D001919:Bradycardia / Q000139:chemically induced; D004146:Dioxanes / Q000494:pharmacology; D006321:Heart / Q000294:innervation / Q000502:physiology*; D006339:Heart Rate / Q000502:physiology; D019329:Idazoxan; D008297:Male; D008845:Microinjections; D009638:Norepinephrine / Q000008:administration & dosage / Q000494:pharmacology*; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology*; D013610:Tachycardia / Q000139:chemically induced
D000317:Adrenergic alpha-Antagonists; D004146:Dioxanes; D009638:Norepinephrine; D011224:Prazosin; D019329:Idazoxan