Correlation between endotoxin-neutralizing capacity of human plasma as tested by the limulus-amebocyte-lysate-test and plasma protein levels.
In the present study the endotoxin-neutralizing capacity of human plasma obtained from healthy volunteers was determined by use of the limulus-amebocyte-lysate test. The extent of the endotoxin-neutralizing capacity which showed a very broad variation was correlated to the plasma levels of some proteins which are believed to contribute to endotoxin transport and detoxification. The plasma levels of alpha 2-macroglobulin and transferrin, the major transport proteins, as well as the levels of IgG, IgA and IgM and apoprotein A were not significantly correlated to the neutralizing capacity. Only the apoprotein B, the major apoprotein of the low density lipoprotein fraction shows a significant correlation. Together with previously published results it should be concluded that low density lipoproteins are involved in the endogenous endotoxin-neutralizing reaction occurring in human plasma, at least when high doses of endotoxin are administered. Nevertheless the endotoxin-neutralizing reaction seems to be a very complex multistep process.
Berger
D
D
Department of General Surgery, University of Ulm, Germany.
Schleich
S
S
Seidelmann
M
M
Beger
H G
HG
D001054:Apolipoproteins A / Q000032:analysis; D001055:Apolipoproteins B / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D004731:Endotoxins / Q000037:antagonists & inhibitors* / Q000097:blood; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D008033:Limulus Test; D008077:Lipoproteins, LDL / Q000032:analysis; D014168:Transferrin / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis
D001054:Apolipoproteins A; D001055:Apolipoproteins B; D001798:Blood Proteins; D004731:Endotoxins; D007136:Immunoglobulins; D008077:Lipoproteins, LDL; D014168:Transferrin; D000511:alpha-Macroglobulins