In vitro selection of RNA molecules that bind specific ligands.
Subpopulations of RNA molecules that bind specifically to a variety of organic dyes have been isolated from a population of random sequence RNA molecules. Roughly one in 10(10) random sequence RNA molecules folds in such a way as to create a specific binding site for small ligands.
Ellington
A D
AD
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Szostak
J W
JW
D001483:Base Sequence; D001665:Binding Sites; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004396:Coloring Agents / Q000378:metabolism*; D004247:DNA / Q000138:chemical synthesis; D008024:Ligands; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014227:Triazines / Q000378:metabolism
D004396:Coloring Agents; D008024:Ligands; D014227:Triazines; C036598:procion blue MX-R; C012842:Cibacron Blue F 3GA; D012313:RNA; D004247:DNA