Effect of isoenzyme composition on Kodak Ektachem test results for creatine kinase, lactate dehydrogenase, alkaline phosphatase, and amylase.
Seventy-four patients' sera containing macroenzymes or other uncommon enzyme forms were analyzed for total creatine kinase, lactate dehydrogenase, alkaline phosphatase, or amylase activity on an Ektachem 700XR analyzer. The Ektachem test results correlated well with activities obtained by means of liquid reagents on RA-1000. For each enzyme tested, linear-regression analysis yielded data comparable with those for sera containing exclusively the more common isoenzymes. Increased concentrations of intestinal ALP, however, resulted in higher activities of total ALP being reported for the Ektachem.
Gorus
F K
FK
Department of Clinical Chemistry, Akademisch Ziekenhuis, Vrije Universiteit Brussel, Belgium.
De Pree
L
L
D000469:Alkaline Phosphatase / Q000097:blood*; D000681:Amylases / Q000097:blood*; D001322:Autoanalysis; D002624:Chemistry, Clinical / Q000592:standards; D003402:Creatine Kinase / Q000097:blood*; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D012044:Regression Analysis
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D000469:Alkaline Phosphatase; D000681:Amylases