Regeneration of teat canal keratin in lactating dairy cows.
This study was designed to determine the regeneration rate of teat canal keratin in two groups of 10 lactating Holstein cows. The weight of keratin obtained upon successive collections of keratin was determined. Intervals between successive collections were varied between 8 and 72 h. Following initial collection, the quantity of keratin removed was regenerated within 1 to 2.5 d. The rate of keratin regeneration per teat was approximately 1.5 mg of wet weight or .6 mg of dry weight per 24 h. The concentration (microgram lipid/mg keratin, wet weight) of lipid in keratin samples collected during regeneration was almost twice that present initially or after regeneration was complete. With the exception of free cholesterol, composition (%) of lipid components of keratin did not differ between samples collected initially, those collected early in the process of regeneration, and those collected after the mass of keratin removed had been replaced. Although data suggest that keratin matures during the process of regeneration, maturation appears complete by the time mass of keratin is fully regenerated. Additionally, the quantity of keratin recovered at initial sampling was inversely correlated with milk production. We hypothesize that as milk production increases, more keratin is lost during milking.
Milk Secretion and Mastitis Laboratory, USDA, ARS, Livestock and Poultry Science Institute, Beltsville, MD 20705.
A V
AV
Capuco
D L
DL
Wood
S A
SA
Bright
R H
RH
Miller
J
J
Bitman
D000818:Animals; D002417:Cattle / Q000502:physiology*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D007633:Keratins / Q000096:biosynthesis* / Q000737:chemistry / Q000502:physiology; D007774:Lactation / Q000502:physiology*; D008055:Lipids / Q000032:analysis; D008321:Mammary Glands, Animal / Q000378:metabolism / Q000502:physiology*; D008892:Milk / Q000378:metabolism; D012038:Regeneration
D005227:Fatty Acids; D008055:Lipids; D007633:Keratins