[The genotyping of reassortants of the influenza virus type B].
A rapid method requiring a small amount of materials is described for genotyping of influenza type B virus reassortants. It allows one to carry out within a short time a simultaneous analysis of the genome of 12 virus strains by PAG electrophoresis of virion RNAs. The conditions for the detection of the greatest strain-specific differences in the mobility of individual RNA segments of influenza B virus were found: 3.3% PAG plus 4.5 M urea, although the most reliable results were obtained by comparisons in gels with various acrylamide-urea ratios.
G M
GM
Vodeĭko
A I
AI
Klimov
D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral / Q000235:genetics; D005821:Genetic Techniques; D005838:Genotype; D006801:Humans; D009981:Influenza B virus / Q000145:classification* / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D011995:Recombination, Genetic; D012834:Silver; D013194:Staining and Labeling / Q000379:methods; D014771:Virion / Q000235:genetics / Q000302:isolation & purification
D012367:RNA, Viral; D012834:Silver
Genotipirovanie reassortantov virusa grippa tipa B.