Human VNTR sequences in porcine HTF-islands.
Mapping of complex genomes has been influenced substantially by the isolation of locus-specific, but repetitive DNA elements known as VNTRs. Since a high GC-content is characteristic of most of these elements one might expect them to be clustered at least partially in CpG-islands. To address this question we have constructed a porcine liver DNA NotI-linkage library in pUC18 using isolated HTF-islands. HpaII tiny fragments ranging from less than 100 bp to 1 kb in length were randomly selected and analysed by sequencing. As expected from the source of DNA the clones were rich in CpG-, C- and G-content. Several clones exhibiting a repetitive (GGC)n-motif and sequences originally present in human VNTR-markers were shown to detect DNA fingerprints in different species (Homo sapiens, Sus scrofa domestica, Gallus domesticus, Glycine max, Saccharomyces cerevisiae). A subset of clones was used for hybridization experiments with porcine DNA and were shown to detect single-copy linkage fragments.
Brenig
B
B
Department of Molecular Animal Breeding, Ludwig-Maximilins-University of Munich, Germany.
Brem
G
G
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016172:DNA Fingerprinting; D004276:DNA, Satellite / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific; D015226:Dinucleoside Phosphates; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D008040:Genetic Linkage; D006801:Humans; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine
D004276:DNA, Satellite; D015252:Deoxyribonucleases, Type II Site-Specific; D015226:Dinucleoside Phosphates; C015772:CpG dinucleotide; C038565:GCGGCCGC-specific type II deoxyribonucleases