Antipeptide antibodies against conserved regions of human interferon-alpha: evidence for conformational variations between IFN-alpha subtypes.
Antibodies to two conserved regions (residues 29-36 and 139-151) of human interferon-alpha were raised by immunizing rabbits with four short synthetic peptides coupled to carriers. The antibodies were tested for reactivity with recombinant interferon-alpha by ELISA. Despite the amino acid conservation of the two regions, there are significant variations in the reactivity of the antibodies with the IFN-alpha subtypes. The reactivity is enhanced significantly when the disulfide bonds of the interferon molecule are reduced. The results indicate that there are subtype-specific differences in the presentation of the epitopes in these conserved regions of human interferon-alpha.
Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
G L
GL
McMullen
N
N
Sattayasai
A R
AR
Hibbs
S
S
Marzuki
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D004220:Disulfides; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006801:Humans; D007370:Interferon Type I / Q000276:immunology*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011817:Rabbits; D011994:Recombinant Proteins
D000906:Antibodies; D004220:Disulfides; D000939:Epitopes; D007370:Interferon Type I; D011994:Recombinant Proteins