Effect of dietary alpha-linolenic acid on endotoxin-induced production of tumor necrosis factor by peritoneal macrophages in horses.
A study was conducted to determine whether dietary supplements with alpha-linolenic acid altered the ability of equine peritoneal macrophages to produce tumor necrosis factor (TNF) in response to endotoxin. Peritoneal macrophages were harvested from 6 healthy adult horses before and after the horses were fed a nutritionally balanced ration that contained 8% linseed oil as a source of alpha-linolenic acid. The macrophages were cultured in media containing no additives (control), endotoxin (0.5 to 50 ng/ml), or the calcium ionophore, A23187. Macrophage supernatants were collected after 6 and 24 hours' incubation and stored at -70 C. Tumor necrosis factor activity was estimated by a modified in vitro cytotoxicity bioassay, using the murine fibrosarcoma cell line, WEHI 164 clone 13. The TNF activity after 6 and 24 hours' incubation was greater in culture media of macrophages exposed to endotoxin than in media from control macrophages. For macrophages cultured in media that contained endotoxin, neither the concentration of endotoxin nor incubation time had any effect on TNF activity. Endotoxin-induced macrophage production of TNF, as determined by measurement of TNF activity, was significantly less after horses were fed the alpha-linolenic acid-rich ration for 8 weeks.
Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
D D
DD
Morris
M M
MM
Henry
J N
JN
Moore
J K
JK
Fischer
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D004731:Endotoxins / Q000097:blood / Q000633:toxicity*; D004926:Escherichia coli; D005260:Female; D006734:Horse Diseases / Q000209:etiology / Q000517:prevention & control; D006736:Horses / Q000378:metabolism*; D008042:Linolenic Acids / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D017962:alpha-Linolenic Acid
D004042:Dietary Fats, Unsaturated; D004731:Endotoxins; D008042:Linolenic Acids; D014409:Tumor Necrosis Factor-alpha; D017962:alpha-Linolenic Acid; D000001:Calcimycin; C044101:endotoxin, Escherichia coli