Amino acid side chain interaction with chelate-liganded crosslinked dextran, agarose and TSK gel. A mini review of recent work.
Protein adsorption and retention data collected from recent chromatographic studies on hydrophilic gels substituted with chelate-bonded metal ions are discussed. Attempts are made to interpret the adsorption behavior in terms of molecular events caused by the affinity for the immobilized metal ions.
University of Arizona, Division of Biotechnology, Tucson 85721.
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J
Porath
D000327:Adsorption; D000596:Amino Acids / Q000302:isolation & purification*; D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods*; D003911:Dextrans; D005782:Gels; D006493:Heparin; D007477:Ions; D008670:Metals; D011108:Polymers; D011506:Proteins / Q000302:isolation & purification; D012685:Sepharose
D000596:Amino Acids; D002614:Chelating Agents; D003911:Dextrans; D005782:Gels; D007477:Ions; D008670:Metals; D011108:Polymers; D011506:Proteins; C058416:TSKgel heparin-5PW; D006493:Heparin; D012685:Sepharose