A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification.
A simple and rapid method for the extraction, reverse transcription and PCR amplification of RNA from formalin-fixed, paraffin-embedded tissue is described. The procedure can be completed within 24 hours. In a first application of this method we detect human albumin mRNA in liver tissue, demonstrating the feasibility to retrospectively analyze gene expression and RNA viruses in fixed tissue.
Massachusetts General Hospital, Harvard Medical School, Charlestown.
F
F
von Weizsäcker
S
S
Labeit
H K
HK
Koch
W
W
Oehlert
W
W
Gerok
H E
HE
Blum
D000418:Albumins / Q000235:genetics; D001707:Biopsy, Needle; D004247:DNA / Q000096:biosynthesis; D005404:Fixatives; D005557:Formaldehyde; D015870:Gene Expression; D006801:Humans; D007438:Introns; D008099:Liver / Q000737:chemistry*; D010232:Paraffin; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012328:RNA Viruses / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D000418:Albumins; D005404:Fixatives; D012333:RNA, Messenger; D005557:Formaldehyde; D010232:Paraffin; D004247:DNA; D012194:RNA-Directed DNA Polymerase