Distribution of two forms of somatostatin and peptides belonging to the pancreatic polypeptide family in tissues of larval lampreys, Petromyzon marinus L.: an immunohistochemical study.
Immunohistochemistry on tissues of larval lampreys, Petromyzon marinus L., was used to determine the distribution of invariant somatostatin-14 (SST-14) and lamprey somatostatin-34 (SST-34) in the brain while antisera against porcine peptide tyrosine tyrosine (PYY), human neuropeptide Y (NPY), anglerfish peptide YG (aPY), salmon glucagon-like peptide (GLP), SST-14, and SST-34 were used in studies of the pancreas and anterior intestine. In the brain, SST-14 is the major form of somatostatin. SST-14- and SST-34-immunoreactive nerve fibers are distributed throughout the telencephalon, diencephalon, and mesencephalon. In the latter region SST-14 immunoreactivity is concentrated in nerve tracts in the nucleus interpeduncularis. Nerve cells within the olfactory bulbs are immunoreactive only to anti-SST-34. Cells immunostained with anti-SST-14 were localized within the ependymal and subependymal layers of the pars ventralis hypothalami and the subependymal layers of the pars dorsalis thalami. SST-14-immunoreactive perikarya are also distributed within the tegmentum mesencephali. Nerve fibers and cells immunoreactive to anti-SST-34 are detected in the pars ventralis hypothalami but these cells do not colocalize SST-14. Pancreatic islets, distributed within the epithelium and in the submucosal connective tissue at the esophageal-intestinal junction, are only immunoreactive to anti-insulin. The antisera revealed three distinct cell types in the intestinal epithelium: type 1 colocalizes aPY, NPY, and PYY; type 2 colocalizes SST-14 and SST-34; and type 3 demonstrates immunoreactivity only to anti-SST-34. Immunoreactivity to anti-GLP is absent.
Cheung
R
R
Department of Zoology, University of Toronto, Ontario, Canada.
Ferreira
L C
LC
Youson
J H
JH
D000818:Animals; D001923:Brain Chemistry; D004027:Diencephalon / Q000737:chemistry; D007124:Immunoenzyme Techniques; D007422:Intestines / Q000737:chemistry; D007798:Lampreys / Q000378:metabolism*; D007814:Larva / Q000032:analysis; D008636:Mesencephalon / Q000737:chemistry; D009478:Neuropeptide Y / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis; D010179:Pancreas / Q000737:chemistry; D010191:Pancreatic Polypeptide / Q000032:analysis*; D019894:Peptide YY; D010455:Peptides / Q000032:analysis*; D013004:Somatostatin / Q000032:analysis*; D013687:Telencephalon / Q000737:chemistry; D014018:Tissue Distribution
D009478:Neuropeptide Y; D009479:Neuropeptides; D010455:Peptides; D019894:Peptide YY; C070440:somatostatin 34; D013004:Somatostatin; D010191:Pancreatic Polypeptide; C061122:anglerfish peptide YG