Mapping of a retinoic acid-responsive element in the promoter region of the complement factor H gene.
Retinoic acid receptors are members of the steroid/thyroid hormone receptor superfamily. Pursuant to the discovery that dexamethasone increases complement factor H expression, we examined the effects of retinoic acid on this gene. Both H mRNA and protein levels are increased by retinoic acid in L cells. Using the luciferase reporter gene system we have identified a region of the H promoter required for the retinoic acid response. This region contains an imperfect palindrome of the TGACC motif, present in thyroid hormone and estrogen-responsive elements. We demonstrate specific binding of the retinoic acid receptor beta to this sequence of the H gene by DNA-protein gel retardation assay. Therefore, these studies extend the sphere of influence of the retinoids to complement, an intrinsic component of the humoral immune system.
Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037.
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Muñoz-Cánoves
D P
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Vik
B F
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Tack
D000818:Animals; D001483:Base Sequence; D003180:Complement C3b Inactivator Proteins / Q000235:genetics*; D017242:Complement Factor H; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes / Q000187:drug effects; D007700:Kinetics; D007739:L Cells / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology*
D003180:Complement C3b Inactivator Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014212:Tretinoin; D012313:RNA; D017242:Complement Factor H