The effect of dextran sulphate on CFU-S and nucleic acids in blood and haemopoietic tissues in irradiated mice.
The effect of synthetic polyanion dextran sulphate on the development and recovery of radiation-induced haemopoietic damage in mice was investigated. Dextran sulphate (mol. wt. 500,000 D) in the dose of 40 mg.kg-1 of body weight was injected i.p. 3 days before single total body irradiation with a dose of 7.8 Gy gamma-rays. The animals were examined from hour 6 to day 26 after irradiation, i.e. from hour 78 to day 29 after DS-treatment. In irradiated mice DS-pretreatment showed some positive effect on the CFU-S number in bone marrow (less in spleen and blood), bone marrow cellularity, attenuated the radiation-induced changes of erythrocytes (number, MCV) and of RNA concentration in blood. The changes of other parameters (spleen cellularity, liver CFU-S, leukocyte count and DNA concentration in blood) were the same as in unprotected animals. In conclusion, we can say that DS-pretreatment had a beneficial effect on the recovery of radiation-induced damage of erythropoiesis but not on granulopoiesis or lymphopoiesis.
Pado
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D
Department of General Biology, Faculty of Science, P. J. Safárik University, Kosice, CSSR.
Misúrová
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Fedoroćko
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P
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D003114:Colony-Forming Units Assay; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D007700:Kinetics; D007962:Leukocytes / Q000187:drug effects / Q000528:radiation effects; D008099:Liver / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008813:Mice, Inbred ICR; D013154:Spleen / Q000187:drug effects / Q000528:radiation effects; D014916:Whole-Body Irradiation
D003037:Cobalt Radioisotopes; D003911:Dextrans; D016264:Dextran Sulfate