Genetic variation of putative core gene in hepatitis C virus.
Genetic variation of hepatitis C virus was assessed. We prepared RNA fractions from 21 patients' sera which were positive for hepatitis C virus RNA, synthesized their cDNAs, and amplified fragments, 406 base pairs, encoding a putative core protein, by polymerase chain reaction. One of them, N 15, was cloned and sequenced. N 15 showed 92.4% homology at the nucleotide level and 97.0% homology at the amino acid level compared with HC-J 1 which is the first isolated clone in Japan and similar to that isolated in USA. By restriction fragment length polymorphisms analysis, 14 out of 21 patients (66.7%) showed the same pattern as N 15. No patients showed the pattern of HC-J 1. We could not find a correlation between the genetic variation and clinical features of hepatitis C virus infection. These results indicate that the region, which encodes the core protein and is believed to be relatively conserved in hepatitis C virus genome, has several variations at the nucleotide level, and the major part of hepatitis C virus in Okayama district is different from HC-J 1 and the USA clone.
First Department of Internal Medicine, Okayama University Medical School, Japan.
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K
Fujio
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Shimomura
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Tsuji
D000328:Adult; D000368:Aged; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D005814:Genes, Viral / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012367:RNA, Viral / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D014758:Viral Core Proteins / Q000235:genetics
D012367:RNA, Viral; D014758:Viral Core Proteins