Immunohistochemical recognition of ethylnitrosourea induced rat brain microtumors by anti-Leu 7 monoclonal antibody.
This immunocytochemical study was undertaken to clarify the histogenesis of ethylnitrosourea-induced rat brain tumors. The tumors induced in offspring of Sprague-Dawley rats injected with ethylnitrosourea on day 18 of gestation were used in these experiments. Controls consisted of pregnant Sprague-Dawley rats similarly injected with saline alone. Both microtumors (less than 1 mm) and macrotumors were examined immunocytochemically. The cells present in both macro- and microtumors were reactive with anti-Leu 7, an antibody which recognizes oligodendrocytes. Intermixed with, but distinct from the tumor cells were glial fibrillary acidic protein positive cells morphologically identical to astrocytes found in other areas distant to tumors in the treated animals, and in controls. These data suggest that both early and late tumors are oligodendrogliomas, not astrocytomas or mixed gliomas, and that the cell of origin of the tumor is the oligodendrocyte rather than an uncommitted stem cell as previously suggested.
Department of Pathology, Case Western Reserve University, Cleveland, OH.
P G
PG
Galloway
M J
MJ
Likavec
G
G
Perry
D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis*; D001932:Brain Neoplasms / Q000032:analysis* / Q000139:chemically induced / Q000473:pathology; D018959:CD57 Antigens; D005038:Ethylnitrosourea; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D009837:Oligodendroglioma / Q000032:analysis* / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018959:CD57 Antigens; D005904:Glial Fibrillary Acidic Protein; D005038:Ethylnitrosourea