Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome.
We have investigated the distribution of transposed Acs in the tomato genome. Our approach has been to clone the regions flanking the T-DNAs and transposed Acs from two transgenic lines of tomato and place these sequences on the tomato restriction fragment length polymorphism (RFLP) map. The distribution of transposed Acs around the T-DNA and at locations unlinked to the T-DNA indicates that Ac transposes to linked and unlinked sites in tomato as it does in maize. The structure and terminal sequence of these cloned elements shows that Ac remains intact after transposition. We discuss these results and their bearing on gene tagging strategies using Ac and Ds.
Plant Gene Expression Center, Albany, California 94710.
B I
BI
Osborne
C A
CA
Corr
J P
JP
Prince
R
R
Hehl
S D
SD
Tanksley
S
S
McCormick
B
B
Baker
D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D009838:Oligodeoxyribonucleotides