Binding of nonspecific cross-reacting antigen, a granulocyte membrane glycoprotein, to Escherichia coli expressing type 1 fimbriae.
Nonspecific cross-reacting antigen (NCA) is a well-characterized membrane glycoprotein on granulocytes, macrophages, and lung epithelium. Structural studies at the protein and genomic levels have revealed that NCA is a member of the immunoglobulin supergene family, and hybridization studies showed that the transcript level of NCA is induced by treatment with gamma interferon. These studies, as well as the expression of NCA on granulocytes, suggest a role for NCA in immune response. For a first step in studying this possible role, we have examined the binding of two glycoforms of NCA designated NCA-50 (Mr, 50,000) and TEX-75 (Mr, 75,000). Here we report the results from binding assays which demonstrate carbohydrate-mediated binding of Escherichia coli expressing type 1 fimbriae and of isolated type 1 fimbriae to NCA-50. TEX-75 did not bind to the purified fimbriae but bound slightly to the bacterial strain. Inhibition studies showed that the binding to NCA-50 involved interaction of mannose moieties on NCA-50 and lectins on the fimbriae. The binding of NCA-50 to bacterial fimbriae was confirmed by electron microscopy studies, using immunolabeling techniques. In addition, we show that the surface expression of NCA-50 (and presumably of other NCA species) on isolated polymorphonuclear leukocytes is increased following activation with the bacterial peptide formylmethionyl-leucyl-phenylalanine, consistent with a role for NCA in immune response.
Sauter
S L
SL
Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California 91010.
Rutherfurd
S M
SM
Wagener
C
C
Shively
J E
JE
Hefta
S A
SA
D000951:Antigens, Neoplasm; D001422:Bacterial Adhesion; D002241:Carbohydrates / Q000032:analysis; D015815:Cell Adhesion Molecules; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D008854:Microscopy, Electron; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D011485:Protein Binding
D000951:Antigens, Neoplasm; D002241:Carbohydrates; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D009240:N-Formylmethionine Leucyl-Phenylalanine