Humoral and cellular immunity following severe head injury: review and current investigations.
Infection is a common and serious complication of severe head injury. Immunocompetence in 25 severely head injured patients was investigated by measuring: (1) delayed-type hypersensitivity (DTH) skin test responses to common antigens; (2) phytohaemagglutinin (PHA) stimulated peripheral blood lymphocyte (PBL): blastogenesis, phenotype expression, and lymphokine production; (3) lymphokine-activated killer (LAK) cytotoxicity, antibody dependent cellular cytotoxicity (ADCC) and natural killer (NK) cytotoxicity; and (4) immunoglobulin and complement levels. The incidence of anergy to DTH skin testing was 100%. There was a decrease in PHA stimulated: PBL blastogenesis (p = 0.002), T-cell expression (p = 0.018), helper T-cell expression (p less than 0.001), interleukin-2 receptor expression (p less than 0.001), interleukin-2 production (p = 0.035) and gamma-interferon production (p less than 0.001). LAK cytotoxicity was depressed following incubation with IL-2 (p less than 0.001). There was no significant decrease in immunoglobulin levels and all acute phase reactants tested increased. The results of this study indicate that the cellular arm of immune response, including lymphocyte activation and cytokine production, is suppressed following severe head injury. The lack of enhancement in LAK cytotoxicity following incubation of PBLs with interleukin-2 suggests that factors other than decreased interleukin-2 production, such as the inherent lymphocyte dysfunction, other soluble mediators or suppressor cells, may be responsible for the reduction in cellular immunity observed following severe head injury.
Miller
C H
CH
Department of Neurosurgery, UC Davis Medical Center, Sacramento 95816.
Quattrocchi
K B
KB
Frank
E H
EH
Issel
B W
BW
Wagner
F C
FC
D000293:Adolescent; D000328:Adult; D000917:Antibody Formation / Q000502:physiology*; D001424:Bacterial Infections / Q000209:etiology; D002453:Cell Cycle / Q000502:physiology; D003165:Complement System Proteins / Q000032:analysis; D006259:Craniocerebral Trauma / Q000150:complications / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular / Q000502:physiology*; D007136:Immunoglobulins / Q000097:blood; D016130:Immunophenotyping; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008934:Mitogens; D011446:Prospective Studies; D012882:Skin Tests / Q000379:methods
D016207:Cytokines; D007136:Immunoglobulins; D008934:Mitogens; D003165:Complement System Proteins