High-performance liquid chromatographic determination of D-amino acid oxidase activity.
A new procedure for the assay of D-amino acid oxidase activity has been developed. alpha-Ketoisovaleric acid, derived from D-valine, was estimated by high-performance liquid chromatography after reaction with o-phenylenediamine to give the corresponding quinoxalinol derivative. alpha-Ketovaleric acid was used as an internal standard to ensure the reproducibility of the method. As an example of application, kidney cortex homogenates were analyzed for their D-amino acid oxidase activity. The advantages of the presented procedure for the determination of the enzymatic activity in biological samples compared with previously reported procedures are discussed.
Institute of Veterinary Physiology and Biochemistry, Milan, Italy.
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Biondi
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Guidotti
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Negri
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D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003605:D-Amino-Acid Oxidase / Q000737:chemistry*; D045782:Hemiterpenes; D007651:Keto Acids / Q000032:analysis / Q000737:chemistry; D007668:Kidney / Q000201:enzymology; D010421:Pentanoic Acids / Q000737:chemistry; D010655:Phenylenediamines; D013552:Swine; D014631:Valerates / Q000737:chemistry
D045782:Hemiterpenes; D007651:Keto Acids; D010421:Pentanoic Acids; D010655:Phenylenediamines; D014631:Valerates; C001505:alpha-ketoisovalerate; D003605:D-Amino-Acid Oxidase