Polyadenylated RNA during DAPI-arrested regeneration of Tetrahymena cilia.
Regeneration of cilia in Tetrahymena cells treated with 1 micrograms/ml of 4',6-diamidino-2-phenylindole (DAPI) takes place about 30 min later then in untreated controls. Analysis of RNA isolated at 50-th min of reciliation, that is when the control cells-but not DAPI-treated ones-have just restored their motility, revealed that; - 1. Two peaks of polyadenylated RNA were present in DAPI-treated cells as well as in the control ones; - 2. The mobility of the polyadenylated RNA was the same in the two compared cell samples; - 3. In wheat germ cell-free translational system these poly (A+)-RNA probably directed the synthesis of alpha- and beta-tubulin; - 4. The amount of polyadenylated RNA in compared cell samples was different, in DAPI-treated Tetrahymena its amount was estimated at 1.43% vs. 1.89% in control ones.
Department of Cell Biology, M. Nencki Institute of Experimental Biology, Warsaw, Poland.
W
W
Krawczyńska
B
B
Kludkiewicz
D000818:Animals; D002923:Cilia / Q000187:drug effects* / Q000502:physiology; D004586:Electrophoresis; D007211:Indoles / Q000494:pharmacology*; D009691:Nucleic Acid Denaturation; D011061:Poly A / Q000032:analysis*; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis*; D012038:Regeneration / Q000187:drug effects; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000235:genetics*; D014404:Tubulin / Q000096:biosynthesis
D007211:Indoles; D014404:Tubulin; D011061:Poly A; C007293:DAPI; D012313:RNA