Production of interferon in mixed leukocyte cultures from two individuals.
No detectable interferon (IFN) was produced when purified leukocytes from individual blood donors were incubated without induction at 10(7) cells/ml at 37 degrees C. Mixing the leukocytes from two donors resulted in the production of IFN that was predominantly IFN-gamma but contained also some IFN-alpha/beta. The titers varied greatly depending on the combination of the two individuals. The amount of IFN produced did not bear a clear correlation to the HLA A, B, or DQ antigenic differences between the two individuals. A difference in one or two DR alleles was not always a sufficient stimulus for abundant IFN production, but more numerous antigenic DR differences tended to increase the IFN response.
National Public Health Institute, Helsinki, Finland.
K
K
Cantell
S
S
Koskimies
S
S
Hirvonen
D006680:HLA Antigens; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D007372:Interferons / Q000096:biosynthesis*; D007962:Leukocytes / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed
D006680:HLA Antigens; D007370:Interferon Type I; D007371:Interferon-gamma; D007372:Interferons