[Methods of isolating hematopoiesis islets from the bone marrow].
The effectiveness of the enzymatic and mechanical methods for isolation of hemopoietic islets (HI) from rat bone marrow was comparatively studied. It has been shown that the use of collagenase brings higher yields of HI than the mechanical treatment of bone marrow, mainly at the expense of associations containing no central phagocytizing macrophages. When the mechanical method of HI isolation was used, erythroid islets associated with mononuclear phagocytes prevailed, while with the enzymatic method mainly erythro-granulocytic islets associated with macrophages or reticular cells were isolated. It has been suggested that granulocytic and erythro-granulocytic associations are less resistant to the mechanical treatment.
Gol'dberg
E D
ED
Dygaĭ
A M
AM
Zakharov
Iu M
IuM
Zakharova
O Iu
OIu
Rassokhin
A G
AG
Shakhov
V P
VP
D000818:Animals; D001854:Bone Marrow Cells; D003470:Culture Media; D015672:Erythroid Precursor Cells / Q000166:cytology*; D006098:Granulocytes / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D066298:In Vitro Techniques; D008297:Male; D003012:Microbial Collagenase / Q000494:pharmacology*; D009499:Neutral Red / Q000494:pharmacology*; D010619:Phenazines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling / Q000379:methods*
D003470:Culture Media; D010619:Phenazines; D009499:Neutral Red; D003012:Microbial Collagenase
Metody vydeleniia gemopoéticheskikh ostrovkov kostnogo mozga.