[The amplification of nucleotide sequences by PCR and the new technics for molecular diagnosis].
The polymerase chain reaction (PCR) is a method for selective amplification of DNA or RNA segments of up to 2 kilobase pairs (kb) or more in length. Repeated cycles of enzymatic primer extension in opposite directions are performed using synthetic oligonucleotides flanking the sequences of interest and the heat-stable DNA polymerase from the archaebacterium Thermus aquaticus (Taq polymerase). The reaction is simple, fast and extremely sensitive, and the DNA or RNA content of a single cell is sufficient to detect a specific sequence. This method has proved useful in simplifying complex analytical protocols in basic and applied molecular biology.
Service de Biochimie, INSERM U91, Hôpital Henri-Mondor, Créteil, France.
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M
Goossens
D004247:DNA / Q000032:analysis; D004252:DNA Mutational Analysis; D005189:False Positive Reactions; D030342:Genetic Diseases, Inborn / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000032:analysis
D012313:RNA; D004247:DNA
L'amplification des séquences de nucléotides par PCR et les nouvelles techniques de diagnostic moléculaire.