Immunocytochemical evidence of vertebrate bioactive peptide-like molecules in the immuno cell types of the freshwater snail Planorbarius corneus (L.) (Gastropoda, Pulmonata).
An immunocytochemical investigation was carried out on round and spreading hemocytes of Planorbarius corneus by using 20 antisera to vertebrate bioactive peptides. The immunotests showed the presence of alpha 1-antichymotrypsin-bombesin-, calcitonin-, CCK-8 (INC)-, CCK-39-, gastrin-, glucagon-, Met-enkephalin-, neurotensin-, oxytocin-, somatostatin-, substance P-, VIP-, and vasopressin-immunoreactive molecules in the spreading hemocytes. The round hemocytes were only positive to anti-bombesin, anticalcitonin, anti-CCK-8 (INC), anti-CCK-39, anti-neurotensin, anti-oxytocin, anti-substance P and anti-vasopressin antibodies. No immunostaining was observed with anti-CCK-8 (Peninsula), anti-insulin, anti-prolactin, anti-thyroglobulin and anti-thyroxin (T4) antibodies. As probably in vertebrates, these bioactive peptides may modulate immuno cell function.
Ottaviani
E
E
Department of Animal Biology, University of Modena, Italy.
Cossarizza
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D001773:Blood Cells / Q000032:analysis*; D001839:Bombesin / Q000032:analysis; D002116:Calcitonin / Q000032:analysis; D002766:Cholecystokinin / Q000031:analogs & derivatives / Q000032:analysis; D006434:Hemocytes / Q000032:analysis*; D007124:Immunoenzyme Techniques; D009496:Neurotensin / Q000032:analysis; D010121:Oxytocin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012844:Sincalide / Q000032:analysis; D012908:Snails / Q000032:analysis*; D013373:Substance P / Q000032:analysis; D014667:Vasopressins / Q000032:analysis
D000911:Antibodies, Monoclonal; D011506:Proteins; D014667:Vasopressins; D013373:Substance P; D009496:Neurotensin; D010121:Oxytocin; C032740:cholecystokinin 39; D002116:Calcitonin; D002766:Cholecystokinin; D012844:Sincalide; D001839:Bombesin