[Optimization of the conditions for the immunochemical nephelometric analysis of IgG, IgA and IgM].
Immunonephelometric analysis (INA) is widely used in practical chemistry. the authors have examined the possibility of using Soviet reagents in an ICS Analyzer Beckman (USA) nephelometer. The effects of polyethylene glycol concentration, the reaction buffer pH and ionic strength on the results of INA were under study. Optimal conditions for INA with the test systems anti-IgA-IgA, anti-IgG-IgG, and anti-IgM-IgM with Soviet reagents have been developed. Lyophilized immunoreagents from the Mancini radial immunodiffusion kit, prepared at the N. F. Gamaleia Research Institute for Epidemiology and Microbiology, can be employed for analysis with this nephelometer.
N V
NV
Sorokina
N I
NI
Zhuravleva
D005069:Evaluation Studies as Topic; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007120:Immunochemistry; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D007202:Indicators and Reagents; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007202:Indicators and Reagents
Optimizatsiia usloviĭ immunokhimicheskogo nefelometricheskogo analiza IgG, IgA i IgM.