Evaluation of intracellular lipids by standardized staining with a Sudan black B fraction.
The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography. The cytophotometric assessment of intracellular neutral lipids, stained with SBB-I, is described and applied to the study of changes in granulocytic neutral lipids in leukemia.
Biophysics Unit, Tata Memorial Centre, Parel, Bombay, India.
H N
HN
Subramaniam
K A
KA
Chaubal
D000650:Amnion / Q000737:chemistry / Q000166:cytology; D001391:Azo Compounds; D002478:Cells, Cultured; D003592:Cytophotometry; D006098:Granulocytes / Q000737:chemistry / Q000166:cytology; D006801:Humans; D008055:Lipids / Q000032:analysis*; D009281:Naphthalenes; D013194:Staining and Labeling
D001391:Azo Compounds; D008055:Lipids; D009281:Naphthalenes; C016118:Sudan Black B