Repair of intrinsic DNA lesions.
The repair of apurinic/apyrimidinic (AP) sites is described. The major pathway involves hydrolysis of the stable phosphodiester bond on the 5' side of the lesion by an AP endonuclease. The 5' terminal deoxyribose-phosphate residue is excised by a separate phosphodiesterase which does not appear to be an exonuclease. Repair replication of the single missing nucleotide residue by a DNA polymerase and ligation complete the excision-repair process. The possibility that minor DNA lesions may accumulate with time in long-lived cells is considered. Such lesions should be chemically stable and should not be recognized by DNA-repair enzymes.
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts., Great Britain.
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D004249:DNA Damage; D045647:DNA Glycosylases; D004260:DNA Repair / Q000502:physiology*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004706:Endodeoxyribonucleases / Q000502:physiology; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins; D006801:Humans; D009699:N-Glycosyl Hydrolases / Q000502:physiology; D010727:Phosphoric Diester Hydrolases / Q000502:physiology
D029968:Escherichia coli Proteins; D004706:Endodeoxyribonucleases; D043223:Deoxyribonuclease IV (Phage T4-Induced); C468972:endonuclease IV, E coli; C026044:deoxyribonuclease V; C058246:DNA deoxyribophosphodiesterase; D010727:Phosphoric Diester Hydrolases; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase