Monoclonal antibody to brain cytoplasmic tetrodotoxin-sensitive protein detects an epitope associated with lymphocytes and involved in lymphocyte activation.
We have previously derived a monoclonal antibody, BIP-4, which is specific to a mammalian brain protein representing a type of sodium channel. Here we show that this antibody detects an epitope associated with lymphocytes and that it triggers a proliferative response of the cells. BIP-4 epitope can be detected on both human peripheral blood and murine splenic mononuclear cells. Surface immuno-globulin-negative (i.e. resting T) lymphocytes are neither bound by the antibody nor proliferate to it. Proliferative response exerted in 7-day cultures of murine splenic mononuclear cells by recombinant interleukin-2 was blocked by BIP-4 antibody. We conclude that the epitope shared by a type of brain sodium channel protein and lymphocyte surface is involved in some, yet unrecognized, step of immune cell activation.
Department of Neurochemistry, A.A. Bogomoletz Institute of Physiology, Academy of Sciences of the Ukrainian S.S.R., Kiev.
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Pinchuk
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Pinchuk
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000502:physiology; D001921:Brain / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D000939:Epitopes; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D011994:Recombinant Proteins; D013779:Tetrodotoxin / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007376:Interleukin-2; D009419:Nerve Tissue Proteins; D011947:Receptors, Antigen, B-Cell; D011994:Recombinant Proteins; D013779:Tetrodotoxin