Spontaneous basophilic foci of hepatocellular alteration in Fischer 344 female rats do not exclude iron.
Iron exclusion has been used to identify foci of hepatocellular alteration (FHA) in rats exposed to known carcinogens. This study investigates whether basophilic FHA occurring spontaneously in aged female Fischer 344 rats also exclude iron. Female rats 514-544 days of age were given supplemental iron by injection for 2 weeks prior to being killed. Serial sections of the liver stained with hematoxylin and eosin or Gomori's stain were examined. Seventy-five basophilic FHA were identified; none excluded iron. It may be possible to differentiate spontaneous basophilic FHA from carcinogen-induced, iron-excluding FHA by the use of this staining method.
Stitzel
K A
KA
Procter & Gamble Company, Cincinnati, OH 45239-8707.
Horn
P A
PA
Ezra
M F
MF
D000818:Animals; D004801:Eosine Yellowish-(YS); D005260:Female; D006416:Hematoxylin; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008113:Liver Neoplasms / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D013194:Staining and Labeling
D007501:Iron; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin