Enhancement of the protective efficacy of inactivated influenza A virus vaccine in aged mice by IL-2 liposomes.
A dose-dependent, vaccine-induced protection of aged and young Balb/c mice against lethal influenza A virus challenge has been demonstrated. Low dose formalin-inactivated influenza A virus vaccine was protective in young mice, but not in aged mice, while a higher dose was protective in both. Administration of low dose vaccine with IL-2 liposomes conferred protection comparable to the high dose in aged mice. Serum neutralizing antibody responses were stimulated by vaccine in a dose-dependent manner while IL-2 liposomes significantly enhanced responses in the low dose paralleled protection in young but not in aged mice. Lung interferon levels paralleled lung virus titres in young but not in aged mice. CTL responses in infected mice were generally higher in young than aged mice. These results demonstrate efficacy of IL-2 liposomes as an adjuvant for influenza virus vaccines in the aged.
Influenza Research Center, Department of Microbiology and Immunology, Baylor College of Medicine, Houston, TX 77030.
I N
IN
Mbawuike
P R
PR
Wyde
P M
PM
Anderson
D000375:Aging / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000917:Antibody Formation; D004305:Dose-Response Relationship, Drug; D009980:Influenza A virus / Q000276:immunology / Q000502:physiology; D007252:Influenza Vaccines / Q000627:therapeutic use*; D007372:Interferons / Q000096:biosynthesis; D007376:Interleukin-2 / Q000008:administration & dosage* / Q000627:therapeutic use; D008081:Liposomes; D008168:Lung / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D015164:Vaccines, Inactivated / Q000627:therapeutic use; D014779:Virus Replication
D000914:Antibodies, Viral; D007252:Influenza Vaccines; D007376:Interleukin-2; D008081:Liposomes; D015164:Vaccines, Inactivated; D007372:Interferons