Four epitopes of Pseudomonas aeruginosa elastase defined by monoclonal antibodies.
Monoclonal antibodies against the elastase of Pseudomonas aeruginosa were produced from spleen cells of BALB/c mice primed with purified elastase of P. aeruginosa and P3-X63-Ag8-U1 myeloma cells. The six clones established generated antibodies which reacted with a 33,000-Da peptide and recognized four different elastase epitopes by a competitive binding enzyme-linked immunosorbent assay. The monoclonal antibodies designated as ELA-17 and ELA-42 that recognize two different epitopes reacted by dot-enzyme immunoassay and by Western immunoblotting with all clinical and International Antigen Typing Scheme strains of P. aeruginosa positive for elastase.
Lagacé
J
J
Department of Microbiology and Immunology, Faculty of Medicine, University of Montreal, Quebec, Canada.
Fréchette
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000939:Epitopes / Q000032:analysis*; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D010196:Pancreatic Elastase / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010196:Pancreatic Elastase