Beta-tubulin epitope expression in normal and malignant epithelial cells.
The expression of a unique beta-tubulin isoform (class III) was monitored in squamous cell carcinoma (SCC) and normal epithelial cells using a monoclonal tubulin antibody called TuJ1. Whole tissue homogenates of SCC, normal tissue, SCC grown in nude mice, and SCC cultured cells were examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot. TuJ1 antibody localization was performed using peroxidase immunostaining on paraffin sections of SCC, normal tissue, nude mouse SCC, and immunofluorescent microscopy of SCC cultured cells. The malignant tissues examined stained positive with TuJ1 and a general beta-tubulin antibody, whereas the normal tissues stained positively only for the general beta-tubulin antibody. TuJ1 epitope expression may be a useful marker for SCCs and may assist in understanding differences between normal and malignant squamous cells.
Scott
C A
CA
Department of Otolaryngology-Head and Neck Surgery, University of Virginia Health Sciences Center, Charlottesville 22908.
Walker
C C
CC
Neal
D A
DA
Harper
C E
CE
Bloodgood
R A
RA
Somers
K D
KD
Mills
S E
SE
Rebhun
L I
LI
Levine
P A
PA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D014408:Biomarkers, Tumor / Q000276:immunology*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D051379:Mice; D008819:Mice, Nude; D014404:Tubulin / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D000939:Epitopes; D014404:Tubulin