[Two cases of ulcerative colitis with colon cancer: immunoperoxidase staining using monoclonal antibodies against gastrointestinal tumor and mucin staining].
Surgical specimens from 2 patients with chronic ulcerative colitis accompanied with colon cancer were evaluated by immunoperoxidative staining using monoclonal antibodies A7 (against human colon cancer), S202 (against human gastric cancer), and anti-carcinoembryonic antigen (CEA). The three monoclonal antibodies were reactive with cancerous tissue, anti-CEA antibody and monoclonal antibody S202 reacted with dysplasia tissues, whereas monoclonal antibody A7 did not. Using high-iron diamine technique for mucosubstances (sialomucin and sulfomucin), cancer and dysplasia showed no secretory elements. Surrounding mucosa showed both sialomucin and sulfomucin secretion.
Okuzumi
J
J
First Department of Surgery, Kyoto Prefectural University of Medicine.
Yokota
T
T
Sawai
K
K
Kondoh
S
S
Kuwata
K
K
Tanaka
M
M
Yamane
T
T
Yamaguchi
T
T
Fujita
Y
Y
Takahashi
T
T
D000230:Adenocarcinoma / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D000911:Antibodies, Monoclonal; D003093:Colitis, Ulcerative / Q000150:complications / Q000473:pathology* / Q000503:physiopathology; D003110:Colonic Neoplasms / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D009077:Mucins / Q000378:metabolism; D052116:Sialomucins; D013194:Staining and Labeling; D013274:Stomach Neoplasms / Q000473:pathology*
D000911:Antibodies, Monoclonal; D009077:Mucins; D052116:Sialomucins; C012564:sulfomucin