Isolation and characterization of human peripheral blood CD4+ T cell clones expressing gamma delta T cell receptors.
Rare T cell clones bearing both CD4 and gamma delta T cell receptors (TcR gamma delta) were obtained from human peripheral blood by cell sorting using anti-CD4 and anti-TCR delta 1 antibodies. All the clones established were reactive with anti-TcR gamma delta 1 antibody, whereas only about 20% of the clones showed reactivity with anti-delta TCS1 antibody. Unlike most CD4+ T cells bearing TcR alpha beta, all the clones tested showed lectin-dependent and anti-CD3 antibody-redirected cytolytic activity. About 60% of the clones exhibited natural killer cell-like activity. Immunoprecipitation analysis of TcR gamma delta showed that each clone expressed either a disulfide-linked or non-disulfide-linked heterodimer consisting of 37-44-kDa TcR gamma and TcR delta chains.
Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, Japan.
S
S
Kyoizumi
M
M
Akiyama
Y
Y
Hirai
Y
Y
Kusunoki
D000911:Antibodies, Monoclonal / Q000276:immunology; D015139:Blotting, Southern; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002999:Clone Cells; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016692:Receptors, Antigen, T-Cell, gamma-delta; D004247:DNA