[Correlation of p105 expression and malignancy in gastric cancers].
The expression of proliferation-associated nuclear antigen p105 of gastric carcinomas was studied by multiparameter flow cytometry and immunohistochemical technique. Content of p105-antigen in cancer cells increased with cell cycle progression, and increased more rapidly in cells in late-S phase than in cells in G0 and early S-phases. Cells in M-phase exhibited a dramatic increase in the amount of the antigen, and the amount of IF intensity of mitotic cells were approximately 5-10-fold greater than that of cells in G1- and S-phases. Immunohistochemical technique demonstrated that patients with lymph node metastasis are more likely to have high p105 positive rates than node-negative patients. The mean p105 positive rates of aneuploid tumors were significantly higher than those of diploid tumors. These results indicate that the measurement of p105 positive rates may be a powerful prognostic indicator of gastric carcinoma.
Second Department of Surgery, Kanazawa University, Japan.
Y
Y
Yonemura
S
S
Oyama
H
H
Kimura
T
T
Kamata
A
A
Yamaguchi
K
K
Miwa
I
I
Miyazaki
D000782:Aneuploidy; D000951:Antigens, Neoplasm / Q000378:metabolism*; D002453:Cell Cycle; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D009687:Nuclear Proteins / Q000378:metabolism*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology
D000951:Antigens, Neoplasm; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen