Repeated DNA in Pneumocystis carinii.
A 16-kb DNA fragment designated Rp3-1 and cloned from the genome of rat-derived Pneumocystis carinii was found to contain sequences that were repeated approximately 70 times per genome. The repeated sequences in Rp3-1 spanned at least 10.4 kb. Sequences in Rp3-1 were present on each of the 16 P. carinii chromosomes resolved by field inversion gel electrophoresis. Most of the P. carinii genomic sequences homologous to those in the Rp3-1 clone appeared to be as long as those in the Rp3-1 clone but were highly polymorphic with respect to restriction enzyme cleavage sites. The Rp3-1 DNA fragment appears to be a member of a family of large, degenerate, dispersed repeats.
Department of Molecular Genetics, Biochemistry, and Microbiology, College of Medicine, University of Cincinnati, Ohio 45267-0524.
S L
SL
Stringer
S T
ST
Hong
D
D
Giuntoli
J R
JR
Stringer
D000818:Animals; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D008040:Genetic Linkage; D011010:Pneumocystis / Q000235:genetics*; D011020:Pneumonia, Pneumocystis / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D011919:Rats, Inbred Strains; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004271:DNA, Fungal