Long-term human B cell lines dependent on interleukin-4 and antibody to CD40.
CD40 is a 45- to 50-kilodalton transmembrane glycoprotein expressed on B lymphocytes, epithelial cells, and some carcinoma cell lines. Human resting B lymphocytes entered a state of sustained proliferation when incubated with both the mouse fibroblastic Ltk- cell line that had been transfected with the human Fc receptor (Fc gamma RII/CDw32) and monoclonal antibodies to CD40. In combination with interleukin-4, factor-dependent long-term normal human B cell lines were generated that were consistently negative for Epstein-Barr viral infection. Thus, cross-linking of CD40 is likely to represent an important phenomenon in the clonal expansion of B cells.
Laboratory for Immunological Research, Dardilly, France.
J
J
Banchereau
P
P
de Paoli
A
A
Vallé
E
E
Garcia
F
F
Rousset
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015703:Antigens, CD / Q000276:immunology / Q000502:physiology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology / Q000502:physiology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D019013:CD40 Antigens; D002455:Cell Division; D005347:Fibroblasts / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000254:growth & development; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D011961:Receptors, Fc / Q000235:genetics / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D019013:CD40 Antigens; D011961:Receptors, Fc; D011994:Recombinant Proteins; D015847:Interleukin-4