Early activation of heat shock genes in H2O2-treated Drosophila cells.
Drosophila cells of a diploid clone derived from line Kc were treated with 1 mM H2O2 for 1 to 20 minutes. Dot blot and Northern blot analysis of RNAs extracted from control and treated cells showed that the transcriptional activation of the 6 heat-shock genes tested was early, and maximal within 5 minutes of H2O2 treatment. Analysis of the kinetics of induction of the heat-shock proteins (hsps) after an exposure to H2O2 of 2 or 5 minutes, followed by removal, suggests that this brief treatment was sufficient to trigger the synthesis of all the hsps, which was maximal 1.5 to 3h after this short H2O2 treatment.
Courgeon
A M
AM
Unité Associée au CNRS 1135, Université Pierre et Marie Curie, Paris, France.
Becker
J
J
Maingourd
M
M
Maisonhaute
C
C
Best-Belpomme
M
M
D000818:Animals; D002460:Cell Line; D015342:DNA Probes; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis; D014158:Transcription, Genetic / Q000187:drug effects
D015342:DNA Probes; D006360:Heat-Shock Proteins; D012313:RNA; D006861:Hydrogen Peroxide