Synthesis of lysyl oxidase in experimental hepatic fibrosis.
The synthesis of lysyl oxidase, which initiates the cross-linking of collagen and elastin, was investigated in carbon tetrachloride (CCl4) induced fibrotic liver of rat. Lysyl oxidase activity of the fibrotic liver was 4 times greater than that of normal liver. mRNAs from the livers of normal and CCl4-treated rats were prepared for in vitro protein synthesis and the products were analyzed by immunoprecipitation with a monoclonal antibody against lysyl oxidase. The mRNAs from the fibrotic liver gave more than 3 times higher level of messenger copies for lysyl oxidase than did mRNAs from normal liver. The molecular weight of the nascent lysyl oxidase was 48,000.
Department of Pathology, Wakayama Medical College, Japan.
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Wakasaki
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Ooshima
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000201:enzymology; D004195:Disease Models, Animal; D005260:Female; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008103:Liver Cirrhosis / Q000201:enzymology*; D014176:Protein Biosynthesis; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D012313:RNA; D000594:Amino Acid Oxidoreductases; D008249:Protein-Lysine 6-Oxidase