Genotoxic activity of 1,3-butadiene and nitrogen dioxide and their photochemical reaction products in Drosophila and in the mouse bone marrow micronucleus assay.
The genotoxic activity of a photochemical reaction mixture of 1,3-butadiene and nitrogen dioxide was investigated in vivo in the mouse bone marrow micronucleus assay and the somatic mutation and recombination test in Drosophila (the wing spot test). Butadiene alone was not mutagenic in Drosophila, but induced micronuclei in mice at 10 ppm after 23 h of exposure. Nitrogen dioxide was not genotoxic in either test system. The photochemical reaction products were toxic but probably not mutagenic in Drosophila and not genotoxic in mouse bone marrow. The in vivo results do not confirm earlier in vitro results that demonstrated a strong direct-acting mutagenic activity of the photochemical products in Salmonella.
Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
K
K
Victorin
L
L
Busk
H
H
Cederberg
J
J
Magnusson
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002070:Butadienes / Q000633:toxicity*; D004330:Drosophila / Q000187:drug effects / Q000235:genetics; D005260:Female; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens; D009585:Nitrogen Dioxide / Q000633:toxicity*; D010777:Photochemistry; D010858:Pigmentation / Q000187:drug effects / Q000235:genetics; D011995:Recombination, Genetic / Q000187:drug effects
D002070:Butadienes; D009153:Mutagens; C031763:1,3-butadiene; D009585:Nitrogen Dioxide