Inducible gene expression of activin A/erythroid differentiation factor in HL-60 cells.
Treatment of HL-60 cells with 12-O-tetradecanoyl-phorbol 13-acetate (TPA) for 48 h induced expression of mRNA of beta A chain of activin A/erythroid differentiation factor. Under the same condition, interferon-gamma caused a slight increase in beta A chain mRNA, whereas 1 alpha, 25-dihydroxyvitamin D3, dimethylsulfoxide and all-trans-retinoic acid failed to induce this mRNA in HL-60 cells. Furthermore, 4 h-treatment with TPA or lipopolysaccharide (LPS) induced a marked increase in beta A chain mRNA levels in interferon-gamma-pretreated HL-60 cells. In the cells pretreated with 1 alpha, 25-dihydroxyvitamin D3, TPA and LPS induced as little increase in beta A chain mRNA as in the control cells. Neither alpha nor beta B chain mRNA was detected in any sample. These results indicate that interferon-gamma has a priming effect on the activation of activin A/erythroid differentiation factor gene by TPA or LPS in HL-60 cells.
Fourth Department of Internal Medicine, University of Tokyo School of Medicine, Japan.
S
S
Takahashi
T
T
Yamashita
Y
Y
Eto
H
H
Shibai
K
K
Miyamoto
E
E
Ogata
D028341:Activins; D002454:Cell Differentiation; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007265:Inhibins / Q000235:genetics*; D015473:Leukemia, Promyelocytic, Acute; D008070:Lipopolysaccharides / Q000494:pharmacology; D046911:Macromolecular Substances; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology
D008070:Lipopolysaccharides; D046911:Macromolecular Substances; D012333:RNA, Messenger; D028341:Activins; D011061:Poly A; D014212:Tretinoin; D007265:Inhibins; D012313:RNA; D013755:Tetradecanoylphorbol Acetate