Methylsulphone metabolites of m-dichlorobenzene as ligands for alpha 2u-globulin in rat kidney and urine.
1. Kidneys from rats given oral doses of either 3,5- or 2,4- dichlorophenyl[14C]methylsulphide contained the corresponding sulphones that were associated with a protein which has been isolated and characterized by SDS-polyacrylamide gel electrophoresis and immunoblot analysis to be alpha 2u-globulin. 2. The same methylsulphone-alpha 2u-globulin complex was isolated from the urine from rats doses with 3,5-dichlorophenyl[14C]methylsulphide (0.3-1.0% of dose, 8.5% of 14C in urine. 3. The stoichiometry of binding of the isolated methylsulphone-alpha 2u-globulin complex was shown to be 0.54-0.78 nmol/nmol protein. This binding was not covalent.
Biosciences Research Laboratory, ARS, US Department of Agriculture, Fargo, ND.
G L
GL
Larsen
J E
JE
Bakke
J K
JK
Huwe
D000510:Alpha-Globulins / Q000378:metabolism* / Q000652:urine; D000818:Animals; D002722:Chlorobenzenes / Q000378:metabolism*; D002845:Chromatography; D004121:Dimethyl Sulfoxide; D004586:Electrophoresis; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013450:Sulfones / Q000378:metabolism* / Q000652:urine; D015262:Xenobiotics
D000510:Alpha-Globulins; D002722:Chlorobenzenes; D013450:Sulfones; D015262:Xenobiotics; C013803:alpha 2u globulin; C042146:1,3-dichlorobenzene; C025910:dimethyl sulfone; D004121:Dimethyl Sulfoxide