Oncogenicity of the ret transforming gene in MMTV/ret transgenic mice.
We have successfully produced transgenic mice that carry the ret oncogene driven by a mouse mammary tumor virus promoter/enhancer. Mammary and salivary gland adenocarcinomas were developed in a stochastic fashion in these mice. Moreover, premalignant tumors with hyperplastic and dysplastic lesions of Harderian glands and male reproductive tracts frequently occurred at young ages. High expression of the transgene was closely associated with the development of these tumors although the levels of the transgene expression were variable among individuals. In addition, large amounts of phosphotyrosine-containing proteins were detected in cell lysates from mammary and salivary adenocarcinomas by immunoblotting with the anti-phosphotyrosine antibody.
Department of Immunology, Nagoya University School of Medicine, Japan.
T
T
Iwamoto
M
M
Takahashi
M
M
Ito
M
M
Hamaguchi
K
K
Isobe
N
N
Misawa
J
J
Asai
T
T
Yoshida
I
I
Nakashima
D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D029721:Drosophila Proteins; D004742:Enhancer Elements, Genetic; D005260:Female; D015967:Gene Expression Regulation, Viral; D008297:Male; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009374:Neoplasms, Experimental / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D009857:Oncogenes; D010375:Pedigree; D010641:Phenotype; D019000:Phosphotyrosine; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051096:Proto-Oncogene Proteins c-ret; D011519:Proto-Oncogenes; D015347:RNA Probes; D020794:Receptor Protein-Tyrosine Kinases; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
D029721:Drosophila Proteins; D011518:Proto-Oncogene Proteins; D015347:RNA Probes; D019000:Phosphotyrosine; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases; C416649:Ret protein, Drosophila; C494999:Ret protein, mouse