Transformed SV3T3 cells have a reduced lysosomal compartment and lower levels of enzyme activity than 3T3 cells.
The secreted and intracellular activities of a number of lysosomal hydrolases were higher in 3T3 cells than in SV40-transformed cells. The number of lysosomes and their total volume were also much larger in 3T3 cells and the surface area of their lysosomal membranes was almost twice that of SV3T3 cells. These differences alone were not sufficiently large, however, to account for the disparity seen in activity of some enzymes. Gel electrophoresis showed that a number of protein components present in lysosomal membranes purified from 3T3 cells were absent from SV3T3 membrane preparations. The absence of these components may be correlated with the reduced enzyme activity of SV3T3 cells particularly with respect to beta-glucosidase and acid phosphatase, both of which are normally found associated with lysosomal membranes.
Kennedy Institute of Rheumatology, Hammersmith, London, United Kingdom.
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D000135:Acid Phosphatase / Q000378:metabolism; D000165:Acridine Orange; D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003592:Cytophotometry; D006867:Hydrolases / Q000378:metabolism*; D007425:Intracellular Membranes / Q000201:enzymology / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology* / Q000648:ultrastructure; D011205:Povidone / Q000378:metabolism; D013539:Simian virus 40 / Q000502:physiology; D013194:Staining and Labeling; D001617:beta-Glucosidase / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D006867:Hydrolases; D000135:Acid Phosphatase; D001617:beta-Glucosidase; D001619:beta-N-Acetylhexosaminidases; D000165:Acridine Orange; D011205:Povidone