Detection and characterization of a sensory microganglion associated with the spinal accessory nerve: a scanning laser confocal microscopic study of the neurons and their processes.
The spinal accessory nerve has been generally thought to be a cranial nerve with purely motor function, innervating the trapezius and sternocleidomastoid muscles. The present study identified clusters of sensory neurons consistently associated with this cranial nerve in adult rats. Either a single microganglion or several dispersed microganglia were found that adhered to the spinal root of the nerve, to small vessels, or were free within the subarachnoid space. The neurons of the ganglion had axons that joined the spinal root of the nerve proximal to its exit from the skull. Additional branches appeared to have an intracranial distribution within the arachnoid of the brainstem and along its vessels. Several findings suggest that the function of the ganglion is sensory and not autonomic. First, the architectural features of neurons within the ganglion (including their size, pseudounipolar morphology, and the lack of synaptic contacts) are similar to those of neurons in other sensory ganglia. Second, substance P and calcitonin gene-related peptide coexist within neurons of the microganglion, whereas markers for the major transmitters found in autonomic ganglia in rats are absent. Third, the expression of peptides in neurons of the ganglion was sensitive to neonatal capsaicin treatment. Finally, neurons within the ganglion were filled with a retrogradely transported dye after injection of the dye into the cervical spinal cord. Although the function of the ganglion is not known, its features are consistent with a role in nociception from the muscles of the spinal accessory complex, and it may be involved in headaches that have an occipital distribution.
Wetmore
C
C
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 55406.
Elde
R
R
D000055:Accessory Nerve / Q000502:physiology / Q000648:ultrastructure; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D005724:Ganglia / Q000166:cytology* / Q000648:ultrastructure; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D007546:Isoquinolines; D007834:Lasers; D008297:Male; D009475:Neurons, Afferent / Q000502:physiology / Q000648:ultrastructure*; D018377:Neurotransmitter Agents / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D007546:Isoquinolines; D018377:Neurotransmitter Agents; D013266:Stilbamidines; C017475:lucifer yellow; D006735:Horseradish Peroxidase; D002211:Capsaicin