Demonstration of cytomegalovirus and retrovirus pseudotypes in cultured guinea pig cells.
A mixed viral infection with a cytomegalovirus and a retrovirus in cultured guinea pig embryo (GPE) cells was investigated. The expression of an endogenous guinea pig retrovirus (GPRV) in cultured guinea pig cells was induced by a medium containing 5-bromo-2'-deoxyuridine and dexamethasone. When the induced GPE cells were superinfected with a guinea pig cytomegalovirus (GPCMV), pseudotype virions were observed. Morphological characterization of both viruses and their locations within infected cells was achieved by examination of thin sections of infected cells with transmission electron microscopy. Immunolabeling with colloidal gold particles, 5 or 15 nm in size, permitted the identification of each virus type using GPCMV- or GPRV-specific polyclonal antibodies and the detection of a population of GPCMV and GPRV particles which expressed antigens of both viruses on their envelopes. Enhanced reverse transcriptase activity of GPRV and reduced infectivity titers of GPCMV was noted in dually infected cultures. These data suggest that interaction between GPCMV and GPRV had occurred in dually infected GPE cells and that expression of GPRV was enhanced.
Yale University School of Medicine, Department of Laboratory Medicine, New Haven, Connecticut 06510.
M B
MB
Lerner-Tung
J Y
JY
Crouch
G D
GD
Hsiung
D000818:Animals; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D003586:Cytomegalovirus Infections / Q000150:complications; D004622:Embryo, Mammalian; D006168:Guinea Pigs; D008854:Microscopy, Electron; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D012192:Retroviridae Infections / Q000150:complications
D012194:RNA-Directed DNA Polymerase