Assignment of Clostridium bryantii to Syntrophospora bryantii gen. nov., comb. nov. on the basis of a 16S rRNA sequence analysis of its crotonate-grown pure culture.
The Clostridium bryantii-Methanospirillum hungatei syntrophic coculture, grown on caproate, was adapted to grow on crotonate. Then, C. bryantii was isolated in pure culture from crotonate bottle plates. A 16S rRNA sequence analysis of the pure subculture revealed that, as a member of the gram-positive phylum, it was not closely related to any of the Clostridium species with which it was compared or to any of the other clusters in the gram-positive phylum with which it was compared. However, it was closely related to another syntrophic fatty acid-degrading bacterium, Syntrophomonas wolfei. On the basis of its phylogeny, physiology, and cell wall ultrastructure, we propose assignment of C. bryantii to Syntrophospora bryantii gen. nov., nov. comb.
Zhao
H X
HX
Department of Animal Sciences, University of Illinois, Urbana 61801.
Yang
D C
DC
Woese
C R
CR
Bryant
M P
MP
D001431:Bacteriological Techniques; D001438:Bacteroidaceae / Q000145:classification*; D002473:Cell Wall / Q000648:ultrastructure; D003013:Clostridium / Q000145:classification*; D003437:Crotonates / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D003437:Crotonates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S