Restriction fragment length polymorphisms associated with the gene for the major intrinsic protein of eye-lens fibre cell membranes in mice with hereditary cataracts.
Cloned cDNAs coding for eye-lens fibre cell-membrane proteins, MIP and MP70, were used to detect restriction fragment length polymorphisms (RFLPs) in genomic DNA from inbred mice with autosomally inherited cataracts. Whereas distinct RFLPs associated with the MIP gene were identified in the Cba Cat and Nct mutants, no such genetic variation was associated with the MP70 gene. RFLPs associated with the mouse MIP gene may provide informative DNA markers in gene linkage studies of murine hereditary cataracts.
Department of Haematology, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, U.K.
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A
Shiels
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CS
Griffin
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Muggleton-Harris
D000818:Animals; D020346:Aquaporins; D015139:Blotting, Southern; D002386:Cataract / Q000235:genetics*; D017630:Connexins; D005136:Eye Proteins / Q000235:genetics*; D007908:Lens, Crystalline / Q000378:metabolism*; D008562:Membrane Glycoproteins; D051379:Mice; D008815:Mice, Inbred Strains; D012150:Polymorphism, Restriction Fragment Length; D000099264:Connexin 50
D020346:Aquaporins; D017630:Connexins; D005136:Eye Proteins; D008562:Membrane Glycoproteins; D000099264:Connexin 50; C030328:aquaporin 0