Prevention of activated neutrophil adhesion to endothelium by soluble adhesion protein GMP140.
Neutrophils and monocytes, but not lymphocytes, adhered strongly to plastic surfaces coated with GMP140, a protein of endothelial cells and platelets. This adhesion of neutrophils was mediated by GMP140 and not by the CD18 integrin complex. By contrast, GMP140 in solution inhibited the CD18-dependent adhesion of tumor necrosis factor-alpha-activated neutrophils to plastic surfaces and resting endothelium, but not of resting neutrophils to tumor necrosis factor-alpha-activated endothelium. Thus, the binding of a soluble form of an adhesion protein selectively inhibited another set of adhesive events. Soluble GMP140 may be important in maintaining the nonadhesiveness of neutrophils in the circulation and may serve to limit inflammatory reactions.
Gamble
J R
JR
Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
Skinner
M P
MP
Berndt
M C
MC
Vadas
M A
MA
D000906:Antibodies / Q000494:pharmacology; D015703:Antigens, CD / Q000502:physiology; D018821:CD18 Antigens; D002448:Cell Adhesion / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000502:physiology*; D006801:Humans; D009504:Neutrophils / Q000502:physiology*; D019007:P-Selectin; D010969:Plastics; D010980:Platelet Membrane Glycoproteins / Q000494:pharmacology*; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins
D000906:Antibodies; D015703:Antigens, CD; D018821:CD18 Antigens; D019007:P-Selectin; D010969:Plastics; D010980:Platelet Membrane Glycoproteins; D016028:Receptors, Leukocyte-Adhesion; D014409:Tumor Necrosis Factor-alpha