Modulation of vitronectin receptor binding by membrane lipid composition.
The vitronectin (Vn) receptor belongs to the integrin family of proteins and although its biochemical structure is fully characterized little is known about its binding affinity and specificity. We report here that Vn receptor binding to different matrix proteins is influenced by the surrounding lipid composition of the membrane. Human placenta affinity purified Vn receptor was inserted into liposomes of different composition: (i) phosphatidylcholine (PC); (ii) PC+phosphatidylethanolamine (PE); (iii) PC+PE+phosphatidylserine (PS) + phosphatidylinositol (PI) + cholesterol (chol). The amount of purified material that could be incorporated into the three lipid vesicle preparations was proportional to the efficiency of the vesicle formation that increased from PC (38%) to PC+PE and PC+PE+PS+PI+chol (about 50%) vesicles. Electron microscopy analysis showed that the homogeneity and size of the three liposome preparations were comparable (20-nm diameter) but their binding capacity to a series of substrates differed widely. Vn receptor inserted in PC liposomes bound only Vn, but when it was inserted in PC+PE and PC+PE+PS+PI+chol liposomes it also attached to von Willebrand factor (vWF) and fibronectin (Fn). Vn receptor had higher binding capacity for substrates when it was inserted in PC+PE+PS+PI+chol than PC+PE liposomes. Antibodies to Vn receptor blocked Vn receptor liposome binding to Vn, vWF, and Fn. The intrinsic emission fluorescence spectrum of the Vn receptor reconstituted in PC+PE+PS+PI+chol liposomes was blue-shifted in relation to PC liposomes, suggesting a conformational change of the receptor in the membranes. These data provide direct evidence that the Vn receptor is "promiscuous" and can associate with Vn, vWF and Fn. The nature of the membrane lipid composition surrounding the receptor could thus influence its binding affinity, possibly by changing its conformation or exposure or both.
Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
G
G
Conforti
A
A
Zanetti
I
I
Pasquali-Ronchetti
D
D
Quaglino
P
P
Neyroz
E
E
Dejana
D000595:Amino Acid Sequence; D000906:Antibodies; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D008081:Liposomes; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis; D010743:Phospholipids / Q000494:pharmacology*; D010920:Placenta / Q000276:immunology; D011247:Pregnancy; D011485:Protein Binding; D011487:Protein Conformation; D011971:Receptors, Immunologic / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D019030:Receptors, Vitronectin; D013050:Spectrometry, Fluorescence
D000906:Antibodies; D008081:Liposomes; D009842:Oligopeptides; D010743:Phospholipids; D011971:Receptors, Immunologic; D019030:Receptors, Vitronectin