Validation of a solid-phase extraction high-performance liquid chromatographic assay for doxazosin.
The determination of doxazosin by high-performance liquid chromatography with fluorescence detection is described. Propanolol was used as the internal standard. Plasma samples were treated with methanol to precipitate the proteins. Doxazosin was isolated with C18 reversed-phase extraction columns. The determination limit is 1 ng/ml of plasma, while the extraction columns can be reused frequently. The method is applied to clinical trial samples.
Department of Clinical Pharmacology and Therapeutics, Austin Hospital, Heidelberg, Victoria, Australia.
G P
GP
Jackman
F
F
Colagrande
W J
WJ
Louis
D000317:Adrenergic alpha-Antagonists / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D017292:Doxazosin; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D011224:Prazosin / Q000031:analogs & derivatives* / Q000097:blood; D011433:Propranolol
D000317:Adrenergic alpha-Antagonists; D011433:Propranolol; D017292:Doxazosin; D011224:Prazosin; D000432:Methanol