Monoclonal IgE-mediated cardiac hypersensitivity reactions in the guinea-pig.
Isolated heart preparations were prepared from guinea-pigs passively sensitized with mouse anti-DNP monoclonal IgE to study hemodynamic parameters and histamine release upon antigen challenge (DNP-BSA). In the best experimental conditions a triphasic response (increase, decrease, increase) in heart rate and in coronary flow occurred during 10 minutes after challenge. Coronary flow dramatically decreased (50%) at 2.5 min flanked by two periods of increase in flow and tachycardia (+ 55% of the basal heart rate). Contractile force decreased and remained at 80% of its initial value. Histamine is released within 2 minutes after antigen injection. After this period, the observed hemodynamic modifications seem to be due to other anaphylactic mediators such as prostaglandins, leukotrienes and most probably paf-acether.
Denizot
Y
Y
INSERM U.200, Clamart, France.
Boudet
J
J
Burtin
C
C
Marro
I
I
Benveniste
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003326:Coronary Circulation; D004140:Dinitrophenols / Q000276:immunology; D006168:Guinea Pigs; D006321:Heart / Q000503:physiopathology*; D006339:Heart Rate; D006439:Hemodynamics; D006636:Histamine Release; D006967:Hypersensitivity / Q000276:immunology / Q000503:physiopathology*; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000276:immunology*; D007700:Kinetics; D008297:Male; D009200:Myocardial Contraction; D009206:Myocardium / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
D000911:Antibodies, Monoclonal; D004140:Dinitrophenols; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E