Monoclonal antibodies to the bovine immunoglobulin G2a allotypes A1 and A2.
Production of murine monoclonal antibodies (Mabs) to bovine IgG2a-A1 and A2 allotypes resulted in three Mabs being selected as anti-IgG2a-A1 and A2 reagents. Two Mabs recognize the A1 allotype and 1 recognizes the A2 allotype. Initial epitope mapping with the Mabs indicates that one of the A1 epitopes resides in the hinge region and the other epitope resides more toward the C-terminus of the immunoglobulin. The A2 epitope recognized by the A2 Mab does not appear to reside in the hinge region of the immunoglobulin but apparently resides more C-terminal.
Department of Veterinary Pathology, College of Veterinary Medicine, Texas A & M University, College Station.
D M
DM
Estes
J W
JW
Templeton
L J
LJ
Adams
D000483:Alleles; D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D006825:Hybridomas; D007126:Immunoglobulin Allotypes / Q000235:genetics*; D007074:Immunoglobulin G / Q000235:genetics*; D066298:In Vitro Techniques; D051379:Mice
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G