Broadly neutralizing antibodies elicited by the hypervariable neutralizing determinant of HIV-1.
The principal neutralizing determinant (PND) of human immunodeficiency virus (HIV)-1 resides within the V3 loop of the envelope protein. Antibodies elicited by peptides of this region were able to neutralize diverse isolates. Serum from one of three animals immunized with the human T cell lymphoma virus (HTLV)-IIIMN PND peptide, RP142, neutralized MN and the sequence-divergent HTLV-IIIB isolate. Serum from one of three animals immunized with a 13-amino acid IIIB PND peptide (RP337) also neutralized both of these isolates. Characterization of these sera revealed that the cross-neutralizing antibodies bound the amino acid sequence GlyProGlyArgAlaPhe (GPGRAF) that is present in both isolates. This sequence is frequently found in the PNDs analyzed in randomly selected HIV-1 isolates. Sera from two rabbits immunized with a peptide containing only the GPGRAF residues neutralized divergent isolates, including IIIB and MN.
Department of Surgery, Duke University Medical School, Durham, NC 27710.
K
K
Javaherian
A J
AJ
Langlois
G J
GJ
LaRosa
A T
AT
Profy
D P
DP
Bolognesi
W C
WC
Herlihy
S D
SD
Putney
T J
TJ
Matthews
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D000595:Amino Acid Sequence; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D006168:Guinea Pigs; D015483:HIV Antibodies / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011817:Rabbits; D014759:Viral Envelope Proteins / Q000276:immunology
D000939:Epitopes; D015483:HIV Antibodies; D015488:HIV Antigens; D007106:Immune Sera; D014759:Viral Envelope Proteins