Detection of a T cell receptor delta chain with an anti-TCR alpha chain serum.
Two types of T cell antigen-specific receptors have been described. Most peripheral blood T lymphocytes express, at their surface, an antigen receptor consisting of alpha and beta subunits, while a small subset of thymocytes and a minority of mature T lymphocytes express a heterodimeric receptor termed gamma delta. Whereas the gene segments localization corresponding to the TCR gamma and beta chains are separate, genes encoding the joining and the constant regions of TCR delta chain are located between the TCR V alpha region and the J alpha-C alpha gene cluster. To determine whether V alpha gene segments are used by delta chains, immunoprecipitations from human TCR gamma delta expressing cell clones were performed with an anti-alpha serum. The results show that a rabbit antiserum raised against the purified REX TCR alpha subunit immunoprecipitates a TCR delta chain from the cell surface of only one human T cell clone termed SO1. However, since no SO1 RNA hybridization is observed with REX TCR V alpha probe and SO1 cloned cells do react with an anti-V delta 2 monoclonal antibody, we conclude that TCR delta and alpha chains expressed a limited structural homology and that REX TCR V alpha gene do not seem to be frequently used in a functional delta chain.
Unité INSERM U93, Hôpital Saint-Louis, Paris, France.
G
G
Leca
J C
JC
Bories
F
F
Davi
A
A
Bensussan
D000888:Antibodies, Anti-Idiotypic; D015152:Blotting, Northern; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000378:metabolism*; D007137:Immunoglobulin alpha-Chains / Q000276:immunology / Q000378:metabolism*; D007138:Immunoglobulin delta-Chains / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D000888:Antibodies, Anti-Idiotypic; D007143:Immunoglobulin Heavy Chains; D007137:Immunoglobulin alpha-Chains; D007138:Immunoglobulin delta-Chains; D011948:Receptors, Antigen, T-Cell; D012313:RNA