Genetic control of contact photosensitivity to tetrachlorosalicylanilide. I. Preferential activation of suppressor T cells in low responder H-2k mice.
The genetic control of contact photosensitivity (CPS) to 3,3',4',5-tetrachlorosalicylanilide (TCSA) was studied in H-2- and IgH-congenic mice. The H-2 complex was found to play a major role in determining the pattern of responsiveness, whereas the effect of the Igh locus on the response was not apparent. The H-2k haplotype was closely associated with low responders, whereas mice with the H-2b,d alleles were high responders. The responsiveness of the H-2k strains was converted from low to high levels by treatment with cyclophosphamide (CY) before photosensitization. Transfer of spleen cells from photosensitized H-2k mice showed a suppressive effect on the development of the CPS reaction of syngeneic recipients pretreated with CY. These spleen cells contained antigen-specific CD4+, CD8- suppressor T cells (Ts) that functioned in the induction phase of CPS. The in vitro proliferation of immune lymph node T cells pulsed with photohapten-coupled cells plus peritoneal adherent cells was suppressed by the addition of anti-I-A monoclonal antibody in both H-2d and H-2k strains, suggesting that the I-A molecule are responsible for inducing the positive immune response. On the other hand, such proliferation was significantly enhanced by the addition of anti-I-E alpha antibody in H-2k, but not H-2d, mice. This implies that the low responsiveness of CPS in the H-2k strain is due to the preferential activation of Ts via I-Ek molecules. The present observation further supports the important role of the I-E molecule in the suppressor circuit of CPS to TCSA.
Department of Dermatology, Hamamatsu University School of Medicine, Japan.
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Tokura
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Satoh
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Takigawa
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Yamada
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000939:Epitopes; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007126:Immunoglobulin Allotypes; D008213:Lymphocyte Activation; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D010787:Photosensitivity Disorders / Q000139:chemically induced / Q000235:genetics*; D012457:Salicylamides / Q000009:adverse effects*; D012458:Salicylanilides / Q000009:adverse effects*; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D007126:Immunoglobulin Allotypes; D012457:Salicylamides; D012458:Salicylanilides; C003390:3,3',4',5-tetrachlorosalicylanilide