Primary structure of mouse tyrosine hydroxylase deduced from its cDNA.
The cDNAs for tyrosine hydroxylase were cloned from a mouse brain cDNA library by plaque hybridization. Since the longest cDNA clone lacked approximately 150 bp sequence of its N-terminal region, additional 5' region was obtained using polymerase chain reaction. Nucleotide sequence determination of cDNAs revealed that mouse tyrosine hydroxylase m-RNA encodes 498 amino acids with a calculated molecular mass of 55,990. The amino acid sequence of mouse tyrosine hydroxylase is highly homologous to rat (97%) and human (92%) enzymes.
Ichikawa
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S
Department of Biochemistry, Nagoya University School of Medicine, Japan.
Sasaoka
T
T
Nagatsu
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D012335:RNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics*
D015345:Oligonucleotide Probes; D012335:RNA, Ribosomal; D004247:DNA; D014446:Tyrosine 3-Monooxygenase