[The IGSS (immunogold silver stain) method in the study of the immunophenotype of free cells. Electron microscopy study of human venous blood].
In the present study a pre-embedding immunoelectron microscopical method ideally suited to detect suspended cell surface-associated antigens, is described. In this method 5nm colloidal gold particles have been enlarged by means of silver enhancement yielding a large marker that is easily detectable at the TEM level. The present method is particularly suited when investigation are performed with a low percentage of labeled cells as well as low antigen expression.
Istituto di Istologia ed Embriologia Generale dell' Università di Parma.
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Ferrari
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Scandroglio
D000943:Antigens, Differentiation / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping / Q000379:methods*; D007962:Leukocytes / Q000276:immunology / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D012834:Silver; D013194:Staining and Labeling
D000943:Antigens, Differentiation; D012834:Silver
Il metodo IGSS (immunogold-silver staining) nello studio dell'immunofenotipo di cellule libere. Ricerche al microscopio elettronico sul sangue venoso di uomo.