The apocytochrome b gene of Chlamydomonas smithii contains a mobile intron related to both Saccharomyces and Neurospora introns.
The mitochondrial DNA of the two interfertile algal species Chlamydomonas smithii and Chlamydomonas reinhardtii are co-linear with the exception of ca. 1 kb insertion (the alpha insert) present in C. smithii DNA only. In vegetative diploids resulting from interspecific crosses, mitochondrial genomes are transmitted biparentally except for the alpha insert which is transmitted to all C. reinhardtii molecules in a manner reminiscent of the intron-mediated conversion event that occurs at the omega locus in yeast mitochondria, under the action of the I-SceI endonuclease. Here we report that the alpha insert corresponds to a typical group I intron of 1075 bp, inserted within the gene for apocytochrome b and containing a 237 codon open reading frame (ORF). We also report the complete sequence of the apocytochrome b gene of C. smithii. Comparison with the sequence of the same gene in C. reinhardtii reveals the precise intron insertion site. These data, together with the previous genetic data provide the first example of intron mobility in mitochondria of the plant kingdom. The product of the intronic ORF shows 36% amino acid identity with the I-SceI endonuclease whereas the intron ribozyme shows a 60% identity at the nucleotide level with the Neurospora crassa cob.1 intron. The possibility of a recent horizontal transfer of introns between fungi and algae is discussed.
Département de Biologie Moléculaire, Institut Pasteur, Paris, France.
L
L
Colleaux
M R
MR
Michel-Wolwertz
R F
RF
Matagne
B
B
Dujon
D000595:Amino Acid Sequence; D001059:Apoproteins / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D002696:Chlamydomonas / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D003573:Cytochrome b Group / Q000235:genetics*; D045303:Cytochromes b; D004171:Diploidy; D005796:Genes; D007438:Introns; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D009491:Neurospora / Q000235:genetics; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D012313:RNA / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism; D012440:Saccharomyces / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D001059:Apoproteins; D003573:Cytochrome b Group; D012313:RNA; D045303:Cytochromes b