Griffonia simplicifolia agglutinin-2-binding glycoprotein as a novel carbohydrate antigen of human colonic carcinoma.
Griffonia simplicifolia agglutinin-2-binding glycoprotein (GBG) in human colonic carcinoma was examined immunochemically and histochemically, GBG was extracted from colonic carcinoma as a serum-type glycoprotein of 160 kilodaltons. GBG was not identical with carcinoembryonic antigen (CEA), since its molecular weight and localization in tissue sections were different from those of CEA. The non-reducing terminals of GBG probably carry N-acetylglucosamine, but not blood group determinants. Furthermore, GBG was released by phosphatidylinositol-specific phospholipase C from cell membrane. GBG was suggested to be anchored to the membrane via linkage to a glycosyl-phosphatidylinositol molecule. Among colonic carcinoma-associated antigens, serum-type glycoproteins having N-acetylglucosamine at non-reducing terminals have not previously been reported. GBG is a novel carbohydrate antigen of human colonic carcinoma.
Central Clinical Laboratories, Shinshu University Hospital, Matsumoto.
J
J
Nakayama
A
A
Okano
H
H
Maeda
M
M
Miyachi
H
H
Ota
T
T
Katsuyama
M
M
Kanai
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D001789:Blood Group Antigens / Q000276:immunology; D015153:Blotting, Western; D002272:Carcinoembryonic Antigen / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology* / Q000378:metabolism; D003110:Colonic Neoplasms / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009005:Monosaccharides / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D037121:Plant Lectins; D013194:Staining and Labeling
D000906:Antibodies; D000951:Antigens, Neoplasm; D001789:Blood Group Antigens; D002272:Carcinoembryonic Antigen; D002352:Carrier Proteins; D006023:Glycoproteins; C045475:Griffonia simplicifolia lectins; D037102:Lectins; D009005:Monosaccharides; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D006735:Horseradish Peroxidase; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase