Chromatographic recovery of polypeptides from copper-stained sodium dodecyl sulfate polyacrylamide gels.
A procedure of preparative electrophoresis is described in which proteins separated on sodium dodecyl sulfate gels, stained with copper and eluted by simple diffusion, are highly concentrated on a fluorocarbon packing and freed of small molecular weight substances, including sodium dodecyl sulfate and buffer components and gel-related substances. This method can be used for microscale preparations or it can be scaled up to recover milligram amounts of protein. The purified polypeptides, however denatured, are suitable for amino acid sequencing.
Laboratorium voor Morfologie en Systematiek der Dieren, Rijksuniversiteit Gent, Belgium.
J R
JR
Vanfleteren
K
K
Peeters
D002851:Chromatography, High Pressure Liquid; D003300:Copper; D004591:Electrophoresis, Polyacrylamide Gel; D010455:Peptides / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling
D010455:Peptides; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D003300:Copper