A simple biological assay for relaxin measurement.
1. Relaxin (R) is considered a gestation hormone with an insulin-like molecular structure. Its physiological importance is significant in the reproduction process. 2. Different methods of biologically assaying R have been published but electrophysiology techniques on uterus and ileum of rat have never been used. 3. A protein fraction was obtained from ovarian tissue of the rat and used to measure electrophysiological activity in vivo and in vitro. 4. Protein recovered with R activity was similar to that in previous reports. 5. Reduction of 100% in contraction strength and 50% in its frequency was observed in ileum and in uterus respectively; it was only of 60%, but its frequency increased 43%. 6. Methodological considerations and some physiological aspects are discussed.
del Angel Meza
A R
AR
Unidad de Investigación Biomédica de Occidente, I.M.S.S., Facultad de Ciencias, Universidad de Guadalajara, Jalisco, México.
Beas-Zárate
C
C
Alfaro
F L
FL
Morales-Villagran
A
A
D000818:Animals; D001681:Biological Assay; D002850:Chromatography, Gel; D004594:Electrophysiology; D005260:Female; D007082:Ileum / Q000187:drug effects / Q000502:physiology; D007328:Insulin / Q000302:isolation & purification / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D010053:Ovary / Q000737:chemistry; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D012065:Relaxin / Q000032:analysis* / Q000494:pharmacology; D014599:Uterus / Q000187:drug effects / Q000502:physiology
D007328:Insulin; D012065:Relaxin