Characterization of albumin-acacia complex coacervation.
Complex coacervation between oppositely charged albumin and acacia mixtures has been studied, and the applicability of the various theoretical treatments of complex coacervation (the Voorn-Overbeek, Veis-Aranyi, Nakajima-Sato, and Tainaka theories) to this system has been assessed. Under optimum conditions where maximum coacervate yield occurred, the Voorn-Overbeek theory appeared to apply. However, away from the optimum coacervation conditions, coacervate sol formation was observed, which is in accordance with the Veis-Aranyi and Tainaka theories. Microelectrophoretic measurements were used to determine optimum pH and ionic strength conditions for maximum coacervation, based on the method of Burgess & Carless (1984). The effects of pH and ionic strength on coacervate yield are reported. Around the optimum conditions for maximum coacervation a viscous coacervate phase and a relatively clear equilibrium phase are formed.
Burgess
D J
DJ
Department of Pharmaceutics, College of Pharmacy, University of Illinois, Chicago 60612.
Kwok
K K
KK
Megremis
P T
PT
D000045:Acacia; D002626:Chemistry, Pharmaceutical; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D012710:Serum Albumin, Bovine
D012710:Serum Albumin, Bovine