Paramyxovirus membrane protein enhances antibody production to new antigenic determinants in the actin molecule: a model for virus-induced autoimmunity.
Paramyxovirus membrane (M) protein specifically binds to cellular actin but not to bovine serum albumin or myoglobin, as determined by affinity chromatography and enzyme-linked immunosorbent assay. The binding site for M protein on actin is different from the binding sites for antiactin antibodies. The interaction of M protein with actin resulted in production of antibodies to several new antigenic sites on the actin molecule. Five rabbits immunized with actin alone produced antibodies against the N-terminal sequence (residues 1 to 39). Another five rabbits immunized with a mixture of M protein and actin produced antibodies against a C-terminal fragment and a central region as well as the N-terminal fragment. By immunoblotting with proteolytic fragments of actin, the new antigenic sites were located between amino acid residues 40 to 113, 114 to 226, and 227 to 375. Antisera taken from some patients with recent measles virus infections demonstrated antiactin antibodies to sites other than the N-terminal fragment of actin (amino acids 1 to 39). The interaction of paramyxovirus M protein with actin and the subsequent production of antibodies to new antigenic sites may serve as a model for one of the mechanisms of virus-induced autoimmunity.
Department of Biochemistry, University of Alberta, Edmonton, Canada.
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Anomasiri
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Tovell
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Tyrrell
D000199:Actins / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000276:immunology* / Q000378:metabolism; D009522:Newcastle disease virus / Q000276:immunology*; D010449:Peptide Mapping; D011485:Protein Binding; D011817:Rabbits; D014764:Viral Proteins / Q000276:immunology* / Q000378:metabolism
D000199:Actins; D000939:Epitopes; D046911:Macromolecular Substances; D008565:Membrane Proteins; D014764:Viral Proteins