Polymerase chain reaction amplification of rabies virus nucleic acids from total mouse brain RNA.
In an attempt to improve the sensitivity of the rabies genome hybridization test, PCR amplification was used following reverse transcription of rabies RNA extracted from infected brain. Presence of amplified DNA is demonstrated with either cDNA synthesized from the antigenomic primer or from antimessenger primer.
Unité Rage Recherche, Institut Pasteur, Paris, France.
A
A
Ermine
D
D
Larzul
P E
PE
Ceccaldi
J L
JL
Guesdon
H
H
Tsiang
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000382:microbiology; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D005784:Gene Amplification; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D011818:Rabies / Q000175:diagnosis* / Q000382:microbiology; D011820:Rabies virus / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D012313:RNA; D004247:DNA