Substance P-immunoreactive nerve fibers in tracheobronchial lymph nodes of the guinea pig: origin, ultrastructure and coexistence with other peptides.
Double-labeling immunofluorescence of guinea pig tracheobronchial lymph nodes revealed complete coincidence of SP and CGRP immunoreactivities in perivascular nerves and axons of the medullary lymphatic tissue. Additional dynorphin A or cholecystokinin immunoreactivity was seen only in some of the medullary fibers. Ultrastructurally, all SP-immunoreactive axons were unmyelinated and displayed vesicle-containing varicosities. Retrograde neuronal tracing combined with immunohistochemistry revealed a sensory origin from dorsal root ganglia of SP/CGRP-immunoreactive fibers ramifying within paratracheal lymph nodes, and an additional neuronal population being devoid of SP/CGRP immunoreactivity. The findings provide evidence for several types of sensory nerve fibers innervating lymph nodes.
Institut für Anatomie und Zellbiologie, Universität Heidelberg.
R
R
Kurkowski
W
W
Kummer
C
C
Heym
D000818:Animals; D001980:Bronchi; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002766:Cholecystokinin / Q000032:analysis; D004399:Dynorphins / Q000032:analysis; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000032:analysis / Q000294:innervation*; D008297:Male; D009412:Nerve Fibers / Q000032:analysis* / Q000648:ultrastructure; D009479:Neuropeptides / Q000032:analysis*; D013373:Substance P / Q000032:analysis*; D014132:Trachea
D009479:Neuropeptides; D013373:Substance P; D004399:Dynorphins; D002766:Cholecystokinin; D015740:Calcitonin Gene-Related Peptide