[Identification of a protein with properties of tenascin in the matrix of embryonal cartilage tissue and in a culture of human embryonal fibroblasts].
A protein with properties of tenascine has been discovered in extracellular matrix of human embryonic cartilage tissue. Its Mr, as determined by electrophoresis in PAAG with SDS, is 205 and 195 kDa. Immunoblotting with monoclonal antibodies raised against tenascine, revealed 2 precipitation bands, which permitted to identify the discovered protein as tenascine. This is the first report of detection of tenascine in rib cartilage matrix of human embryos. A protein identified as tenascine, has been also detected in a culture of human embryonic fibroblasts.
Safronova
E E
EE
D000911:Antibodies, Monoclonal; D002356:Cartilage / Q000737:chemistry*; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000302:isolation & purification*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D005109:Extracellular Matrix; D016326:Extracellular Matrix Proteins / Q000302:isolation & purification*; D005347:Fibroblasts / Q000737:chemistry*; D006801:Humans; D015151:Immunoblotting; D019063:Tenascin
D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D019063:Tenascin
Obnaruzhenie belka so svoĭstvami tenastsina v matrikse émbrional'noĭ khriashchevoĭ tkani i v kul'ture émbrional'nykh fibroblastov cheloveka.