Flow cytometric analysis of virus-infected cells and its potential use for screening antiviral agents.
Virus-infected cells were analyzed using multiparameter flow cytometry. Two virus-cell systems were investigated: HSV-1-infected VF cells and influenza C virus JHB/1/66-infected MDCK cells. Analysis included the measurement of the appearance of virus specific antigens. On individual cells, with polyclonal antibodies, antigens were first detected at 12 h p.i., and the numbers of labeled cells were followed up to 96 h p.i. The efficacy of four antiviral agents was tested with this system. The results were in good agreement with those of plaque reduction tests and indicated that this new method may be extremely useful for the correlation of viral and cellular events with antiviral activity. Finally, it was demonstrated that infected cells in both systems have a considerably greater volume than non-infected cells.
Abteilung für Virologie, Technischen Universität München, F.R.G.
O A
OA
Steele-Mortimer
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H
Meier-Ewert
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Löser
M J
MJ
Hasmann
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis; D000998:Antiviral Agents; D002470:Cell Survival; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D009982:Gammainfluenzavirus / Q000187:drug effects / Q000276:immunology; D018139:Simplexvirus / Q000187:drug effects / Q000276:immunology; D013194:Staining and Labeling; D013861:Thiocyanates; D013997:Time Factors; D010948:Viral Plaque Assay
D000956:Antigens, Viral; D000998:Antiviral Agents; D005452:Fluoresceins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate