A novel peptide from amyloid P component supports cell attachment.
Amyloid P component is a glycoprotein found in association with connective tissues throughout the body and is also a component of human serum. We have identified a dodecapeptide from amyloid P component which is capable of supporting the attachment of a wide variety of cells to the surface of polystyrene plastic dishes. 83% of the activity is confined to a hexapeptide, FTLCFR. Saturation of cell attachment occurs at a peptide concentration of 100 micrograms/ml used to coat the plastic. These results indicate that the active peptide may represent a functional property of amyloid P component which heretofore has no function.
Peptide and Immunochemistry Unit, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
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S
Dhawan
R L
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Fields
F A
FA
Robey
D000595:Amino Acid Sequence; D000906:Antibodies; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D000939:Epitopes; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010455:Peptides / Q000138:chemical synthesis* / Q000494:pharmacology; D000683:Serum Amyloid P-Component / Q000737:chemistry / Q000235:genetics / Q000502:physiology*
D000906:Antibodies; D000939:Epitopes; D009842:Oligopeptides; D010455:Peptides; D000683:Serum Amyloid P-Component; D004247:DNA