Rapid increases in the membrane expression of neutral endopeptidase (CD10), aminopeptidase N (CD13), tyrosine phosphatase (CD45), and Fc gamma-RIII (CD16) upon stimulation of human peripheral leukocytes with human C5a.
Rapid increases in the membrane expression of C3 receptors on granulocytes and monocytes in response to the anaphylatoxin C5a have previously been described. In this study we demonstrate increases in the membrane expression of neutral endopeptidase (NEP, CD10, CALLA), aminopeptidase N (APN, CD13), tyrosine phosphatase (CD45/CD45Ro) and the Fc R Fc gamma-RIII (CD16) on granulocytes within minutes of treatment with human C5a. Monocytes responded to C5a with increases in CD13 and CD45/CD45Ro. These membrane modulations could be prevented by preincubating the C5a preparations with anti-C5a mAb C17/5 but not by pretreating the cells with cycloheximide. Increases of CD10, CD13, and CD11b but not CD11a (LFA-1) were also observed in leukocytes from patients undergoing hemodialysis with cuprophan membranes. The increase of CD16 on granulocytes was dependent on the presence of plasma during in vitro activation with C5a indicating that plasma contains inhibitors which prevent the previously described loss of Fc gamma-RIII upon stimulation of the cells.
Department of Immunology, University of Göttingen, FRG.
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T
Werfel
G
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Sonntag
M H
MH
Weber
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O
Götze
D000626:Aminopeptidases / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000378:metabolism*; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000378:metabolism*; D018826:CD13 Antigens; D002462:Cell Membrane; D002482:Cellulose / Q000031:analogs & derivatives; D015936:Complement C5a / Q000494:pharmacology*; D006649:Histocompatibility Antigens / Q000378:metabolism*; D006801:Humans; D017493:Leukocyte Common Antigens; D007962:Leukocytes / Q000378:metabolism*; D016177:Macrophage-1 Antigen / Q000378:metabolism; D015260:Neprilysin / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D011951:Receptors, Complement / Q000378:metabolism; D017463:Receptors, Complement 3b; D011961:Receptors, Fc / Q000378:metabolism*; D017452:Receptors, IgG; D006435:Renal Dialysis; D015854:Up-Regulation
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015214:Antigens, Differentiation, Myelomonocytic; D000951:Antigens, Neoplasm; D006649:Histocompatibility Antigens; D016177:Macrophage-1 Antigen; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011961:Receptors, Fc; D017452:Receptors, IgG; D015936:Complement C5a; D002482:Cellulose; C009865:cuprammonium cellulose; D017493:Leukocyte Common Antigens; D017027:Protein Tyrosine Phosphatases; D000626:Aminopeptidases; D018826:CD13 Antigens; D015260:Neprilysin