Analysis and purification of DNA restriction fragments by high-performance liquid chromatography.
The purification and analysis of restriction fragments play a very important role in molecular biology but the traditional assay methods of DNA fragments, based on gel electrophoresis and caesium chloride gradient centrifugation, are time-consuming and difficult to quantify. High-performance liquid chromatography provides an alternative method which allows the direct quantitation of picogram amounts of eluents in short time. In the present work we report the separation of different restriction fragments, the purification of some fragments and the relationship between the length of double-stranded DNA fragments and peak areas.
Mauri
P L
PL
Istituto Tecnologie Biomediche Avanzate, CNR, Milan, Italy.
Pietta
P G
PG
Pace
M
M
D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length; D013056:Spectrophotometry, Ultraviolet
D004247:DNA; D004262:DNA Restriction Enzymes