Characterization of melanotropin-release-inhibiting factor (melanostatin) from frog brain: homology with human neuropeptide Y.
A polypeptide was purified from frog brain extracts on the basis of its ability to inhibit alpha-melanotropin release from perifused frog neurointermediate lobes. Based on Edman degradation, amino acid analysis, and peptide mapping, the primary structure of this frog melanotropin-release-inhibiting factor (melanostatin) was determined to be H-Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Met- Ala-Lys-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg- Tyr-NH2 . Frog melanostatin belongs to the pancreatic polypeptide/neuropeptide Y/peptide YY family, and the structure of this peptide differs from that of human neuropeptide Y by only one amino acid substitution in position 19. A synthetic replicate of frog melanostatin is coeluted with the native peptide on HPLC and is highly potent in inhibiting alpha-melanotropin secretion in vitro (IC50 = 60 nM).
European Institute for Peptide Research, Laboratory of Molecular Endocrinology, Centre National de la Recherche Scientifique, URA 650, Mont-Saint-Aignan, France.
N
N
Chartrel
J M
JM
Conlon
J M
JM
Danger
A
A
Fournier
M C
MC
Tonon
H
H
Vaudry
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D004305:Dose-Response Relationship, Drug; D009075:MSH Release-Inhibiting Hormone / Q000737:chemistry* / Q000494:pharmacology; D008969:Molecular Sequence Data; D011898:Ranidae; D012636:Secretory Rate / Q000187:drug effects; D000521:alpha-MSH / Q000378:metabolism
D000521:alpha-MSH; D009075:MSH Release-Inhibiting Hormone