Relationship of 2',3'-cyclic nucleotide 3'-phosphohydrolase activity of large enveloped RNA viruses to host cell activity.
Purified virions of the large RNA viruses show 2',3'-cyclic nucleotide 3'-phosphohydrolase (3'-CNPase) activity. The 3'-CNPase activity is virion-associated and stimulated by their treatment with nonionic detergents. Cytopathic viruses such as influenza A2 (Singapore/57), NDV, and VSV showed the specific activity of a virion-associated 3'-CNPase equal to or lower than the specific activity of host cell enzyme. Retroviruses are an example of extreme relationship of 3'-CNPase to virion. With the AMV-BAI-A associated enzyme activity increased after Triton X-100 treatment ten times more than that associated with other viruses examined. The specific activity of virus-associated 3'-CNPase was 16-28 times higher than that in chick myeloblasts. BLV showed a very low enzyme activity. The correlation between the activity of cellular 3'-CNPase and virus yield showed that 3'-CNPase could belong to cellular factors influencing virus replication.
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
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Rosenbergová
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Pristasová
D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D009522:Newcastle disease virus / Q000201:enzymology; D009975:Orthomyxoviridae / Q000201:enzymology; D010727:Phosphoric Diester Hydrolases; D012328:RNA Viruses / Q000201:enzymology* / Q000502:physiology; D012190:Retroviridae / Q000201:enzymology; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology; D014779:Virus Replication / Q000502:physiology
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase