Macrophages from C3H/HeJ mice require an additional step to produce monokines: synergistic effects of silica and poly(I:C) in the release of interleukin 1.
Resident macrophages from C3H/HeJ mice, in contrast with those of other strains of mice such as BDF1 mice, did not release a 35 kD m.w. factor having macrophage replacing activity (FRM) or interleukin 1 (IL-1) when, respectively, cultured alone or in the presence of silica. C3H/HeJ macrophages were nevertheless capable of producing an intracellular IL-1-like activity. In addition, after a two-step activation process, macrophages from BDF1 mice spontaneously released IL-1, whereas silica was required to induce the release of IL-1 from similarly treated C3H/HeJ macrophages. Such in vivo primed and in vitro stimulated macrophages failed to release FRM. In contrast, poly(I:C) was able to induce the release of FRM by C3H/HeJ macrophages but not that of IL-1; moreover, the addition of silica to poly(I:C)-stimulated cells led to an IL-1 release similar to that obtained with normal mice treated with silica alone. Since poly(I:C) is able to elicit the production of interferons (IFN), the involvement of IFNs was investigated in poly(I:C) activity. Neither IFN-alpha/beta nor IFN-gamma, when used alone or in the presence of silica, could induce the release of IL-1 by C3H/HeJ macrophages. In addition, antibodies to IFN-alpha/beta and IFN-gamma were unable to affect the poly(I:C) and silica induced release of IL-1. Thus, the signal provided by poly(I:C) does not appear to be mediated by IFN(s).
Souvannavong
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V
C.N.R.S. URA 1116, Institut de Biochimie, Orsay, France.
Adam
A
A
D000818:Animals; D002448:Cell Adhesion; D002850:Chromatography, Gel; D005260:Female; D007372:Interferons / Q000494:pharmacology; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D015846:Monokines / Q000378:metabolism*; D011070:Poly I-C / Q000494:pharmacology*; D012822:Silicon Dioxide / Q000494:pharmacology*
D007375:Interleukin-1; D008070:Lipopolysaccharides; D015846:Monokines; D012822:Silicon Dioxide; D007372:Interferons; D011070:Poly I-C