Differential effects of sodium lauryl sulphate and non-anoic acid on the expression of CD1a and ICAM-1 in human epidermis.
Normal human skin was exposed to two different detergents, sodium lauryl sulphate in distilled water and non-anoic acid in isopropanol at different concentrations. The detergents were applied under occlusion in epicutaneous tests for 24 h and biopsies were taken at 24 or 48 h. Frozen sections were labelled with monoclonal antibodies against CD1a, CD3 and ICAM-1. The evaluation of the labelled sections showed that there were differential effects on the expression of ICAM-1 and CD1a+ cells in epidermis. After non-anoic acid application ICAM-reactivity could not be detected and there was a decrease of staining for CD1a after exposure to 80% non-anoic acid. Sodium lauryl sulphate treatment, however, induced ICAM-1 expression on keratinocytes and had minor effects on the number of CD1a+ cells. ICAM-1 expression was also detected in normal epidermis in 3 of 9 unexposed control biopsies and after occlusion with the vehicles distilled water and isopropanol. An increased amount of CD3+ cells was found in the skin exposed to both detergents. The results show that there are dose and time dependent variations in the epidermal response to irritants which might influence the immunological events taken place in the epidermis.
Department of Dermatology, University Hospital, Uppsala, Sweden.
M
M
Lindberg
G
G
Färm
A
A
Scheynius
D000328:Adult; D015703:Antigens, CD / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000187:drug effects* / Q000276:immunology; D003877:Dermatitis, Contact / Q000209:etiology* / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004817:Epidermis / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D005227:Fatty Acids / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007509:Irritants / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D015603:Keratinocytes / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D013997:Time Factors
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D005227:Fatty Acids; D007509:Irritants; D012967:Sodium Dodecyl Sulfate; C008776:pelargonic acid