CD5 (Ly-1)-negative, conventional splenic B cells make a substantial contribution to the bromelain plaque-forming cell response in CBA and BW mice.
CD5 (Ly-1) B cells are a minor subpopulation in mouse spleen and are thought to be responsible for the production of natural autoantibodies to bromelain-treated autologous erythrocytes (Br-RBC). Here it is shown that substantial numbers of conventional, CD5-negative, splenic B cells also secrete these antibodies in CBA and (NZB x NZW)F1 mice, whereas in NZB and BALB/c mice they are all produced by the CD5 B-cell population. However, stimulation with bacterial lipopolysaccharide in vivo preferentially activates the CD5 B-cell group to anti-Br-RBC antibody secretion.
Division of Clinical Immunology, Kennedy Institute of Rheumatology, London, U.K.
E M
EM
Andrew
W
W
Annis
M
M
Kahan
R N
RN
Maini
D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D000943:Antigens, Differentiation / Q000032:analysis*; D001323:Autoantibodies / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology*; D001963:Bromelains; D018956:CD5 Antigens; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D007075:Immunoglobulin M / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology
D015703:Antigens, CD; D000943:Antigens, Differentiation; D001323:Autoantibodies; D018956:CD5 Antigens; D007075:Immunoglobulin M; D001963:Bromelains