Application of a thermally-reversible polymer-antibody conjugate in a novel membrane-based immunoassay.
We have developed a novel method to immobilize antibodies onto a cellulose acetate membrane using a conjugate of an N-isopropylacrylamide polymer covalently bound to the antibody. When compared with the unconjugated antibody, over 30-fold increase in retention of the antibody on the membrane was observed when it was conjugated to poly (N-isopropylacrylamide). Studies of the polymer-membrane interaction suggest a combination of hydrophobic and ionic forces, especially the former, is responsible for the high retention. We applied this novel immobilization technology in the development of a membrane-based immunoassay.
Monji
N
N
Genetic Systems Corporation, Seattle, WA 98121.
Cole
C A
CA
Tam
M
M
Goldstein
L
L
Nowinski
R C
RC
D000906:Antibodies / Q000032:analysis*; D000911:Antibodies, Monoclonal; D005452:Fluoresceins; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M / Q000032:analysis; D007145:Immunoglobulin kappa-Chains; D008567:Membranes, Artificial; D011108:Polymers; D013050:Spectrometry, Fluorescence / Q000379:methods; D005719:gamma-Globulins
D000906:Antibodies; D000911:Antibodies, Monoclonal; D005452:Fluoresceins; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D008567:Membranes, Artificial; D011108:Polymers; D005719:gamma-Globulins