Improved silver staining of nucleolar organiser regions in paraffin wax sections using an inverted incubation technique.
A new simple modification to the silver staining of nucleolar organiser regions (AgNORs) was devised which, by performing the incubation with the slide inverted, results in minimal undesirable background staining, a persistent problem. Inverted incubation is facilitated by the use of a commercially available plastic coverplate. This technique has several additional advantages over other published staining protocols. In particular, the method is straightforward, fast, and maintains a high degree of contrast between the background and the AgNORs.
Department of Pathology, Ninewells Hospital and Medical School, Dundee.
G
G
Coghill
A
A
Grant
J M
JM
Orrell
J
J
Jankowski
A T
AT
Evans
D006801:Humans; D008545:Melanoma / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*
D012834:Silver