[Monoclonal antibodies to the species-specific and group antigens of Rickettsia prowazekii].
A number of hybridomas to different R. prowazekii determinants were obtained by the hybridization of spleen cells of BALB/c mice immunized with R. prowazekii corpuscular and soluble antigens. Some of the monoclonal antibodies (McAb) reacted with R. prowazekii thermolabile species-specific protein and did not react with R. typhi antigens (McAb of batches B4/4 and A-D3). McAb C5/2 and A3/2 reacted with the group thermostable antigen, common for R. prowazekii and R. typhi. McAb to the species-specific thermolabile antigen belonged to IgG2a. The McAb thus obtained permit the identification of R. prowazekii and R. typhi and the solution of the problem of the intragroup differentiation of rickettsiae belonging to the typhus group.
E I
EI
Drobyshevskaia
Iu A
IuA
Nedialkov
S V
SV
Spitsyn
V A
VA
Makarova
R P
RP
Diuĭsalieva
I V
IV
Tarasevich
V G
VG
Nesterenko
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D012283:Rickettsia prowazekii / Q000276:immunology*; D012995:Solubility; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes
Monoklonal'nye antitela k vidospetsificheskomu i gruppovomu antigenam Rickettsia prowazekii.