Effect of heparin on the binding affinity of acidic FGF for the cloned human FGF receptors, flg and bek.
Heparin potentiates the mitogenic activity of acidic fibroblast growth factor (aFGF) by 20-100 fold but mechanisms detailing this potentiation have not yet been fully elucidated. We report that heparin increases the binding affinity of aFGF for the two cloned and overexpressed human FGF receptors, flg and bek, by 2-3 fold. This increase in binding affinity, together with previous data demonstrating a 3-5 fold increase in the stability of aFGF, are likely to account for a significant portion of heparin's potentiation of aFGF activity observed in biological assay systems.
Rhône-Poulenc Rorer Central Research, King of Prussia, PA 19406.
J M
JM
Kaplow
F
F
Bellot
G
G
Crumley
C A
CA
Dionne
M
M
Jaye
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism*; D000091344:Filaggrin Proteins; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D051379:Mice; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins / Q000378:metabolism
C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins; D016220:Fibroblast Growth Factor 1; D006493:Heparin