Functional comparison of transactivation by human retrovirus rev and rex genes.
The effect of rev-responsive element deletion on human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) gene expression was examined. The phenotypes of HIV-1 and HIV-2 provirus DNAs lacking the rev-responsive element, as determined by transfection experiments, were indistinguishable from those of virus DNAs carrying rev gene mutations. By using rev-response elements derived from these two viruses, we developed two monitoring systems to evaluate the functionality of HIV-1 rev, HIV-2 rev, and human T-lymphotropic virus type I rex. In both systems, HIV-1 rev and human T-lymphotropic virus type I rex transactivated HIV-2 very efficiently. On the contrary, HIV-2 rev and human T-lymphotropic virus type I rex were poor activators of HIV-1. No functional replacement of rex by HIV-2 rev was observed.
Sakai
H
H
Laboratory of Gene Analysis, Kyoto University, Japan.
Siomi
H
H
Shida
H
H
Shibata
R
R
Kiyomasu
T
T
Adachi
A
A
D015152:Blotting, Northern; D002460:Cell Line; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003110:Colonic Neoplasms; D004279:DNA, Viral / Q000235:genetics; D016355:Genes, pX; D015757:Genes, rev; D015497:HIV-1 / Q000235:genetics*; D015498:HIV-2 / Q000235:genetics*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D011533:Proviruses / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D015533:Transcriptional Activation
D004279:DNA, Viral; D012194:RNA-Directed DNA Polymerase