Bradykinin antibodies: new developments.
Because all antibodies to bradykinin described to date more or less cross-react with larger and smaller bradykinin derivatives, the aim of the present study was the induction of a specific antibody against bradykinin. A bradykinin derivative, containing a Cys residue at position 6 instead of Ser, was linked to BSA by using a new heterobifunctional cross-linking reagent, the N-maleimido-6-aminocaproyl ester of 1-hydroxy-2-nitro-4-benzenesulfonic acid (mal-sac-HNSA). The Cys6-bradykinin derivative conjugated via the SH group to BSA was used to elicit bradykinin-specific antibodies in rabbits. After the fifth booster injection, the cross-reactivity of this antiserum with kallidin is 4 X 10(-3) when compared with bradykinin. The cross-reactivity of the de-Arg1-bradykinin derivative was 2 X 10(-4), indicating that the antibody requires the free N-terminus. The cross-reactivity of bradykinin and de-Arg9-bradykinin was nearly identical. However, de-Arg9-de-Phe8-bradykinin has a binding of 6 X 10(-3). From these data, it can be concluded that due to the ring-like structure of bradykinin, the coupling of bradykinin in the center of the molecule will elicit an antiserum that needs both the free N-terminal as well as the C-terminal phenylalanine and arginine residues for antibody recognition.
Department of Pharmacology, University of Heidelberg, F.R.G.
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U
Hilgenfeldt
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R
Linke
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W
König
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D001920:Bradykinin / Q000031:analogs & derivatives / Q000276:immunology*; D007457:Iodine Radioisotopes; D008969:Molecular Sequence Data; D011817:Rabbits; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000276:immunology
D000906:Antibodies; D007457:Iodine Radioisotopes; C026201:bradykinin, Tyr-; D012710:Serum Albumin, Bovine; C018103:bradykinin, Met-Lys-; D001920:Bradykinin