RNA sequence and the nature of the CuA-binding site in cytochrome c oxidase.
For cytochrome c oxidase subunit II (COXII), DNA and protein sequences suggest that Met-207 (bovine numbering) is conserved in all species except plants. Sequencing of plant mitochondrial COXII mRNAs now indicates that Met-207 is also conserved among plants as a result of a C-to-U type of RNA editing. Considering the strict evolutionary conservation of Met-207 and the homology of COXII to type I (blue) copper proteins and nitrous oxide reductase, we propose a model in which Met-207 is associated with the CuA-binding site (along with Cys-196, Cys-200 and His-204) and plays a role in determining its reduction potential and stability.
Covello
P S
PS
Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada.
Gray
M W
MW
D001483:Base Sequence; D003300:Copper / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism*; D008715:Methionine; D008969:Molecular Sequence Data; D010944:Plants; D012313:RNA / Q000235:genetics
D012313:RNA; D003300:Copper; D008715:Methionine; D003576:Electron Transport Complex IV