Effects of histamine, granulocyte colony-stimulating factor and db-cAMP on the differentiation of HL-60 cells.
Histamine (10(-7) to 10(-3) M) dose-dependently decreased the incorporation of 3H-thymidine into HL-60 cells, starting from the third day of culture. Also, histamine 1 microM significantly induced the differentiation of HL-60 into cells having the morphological characteristics of mature neutrophils, as did db-cAMP and granulocyte colony-stimulating factor (G-CSF). Phosphorylation of proteins appeared at 60 kd and 120 kd was diminished. The phosphorylation profiles induced by G-CSF and db-cAMP were similar to that of histamine. In the case of db-cAMP, however, no phosphorylated band appeared at 120 kd.
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
K
K
Tasaka
N
N
Nakaya
T
T
Nonaka
D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D016179:Granulocyte Colony-Stimulating Factor; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism; D010766:Phosphorylation; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D003115:Colony-Stimulating Factors; D009363:Neoplasm Proteins; D016179:Granulocyte Colony-Stimulating Factor; D003994:Bucladesine; D006632:Histamine; D013936:Thymidine