Characterization of two human lymphoid cell lines producing human T-lymphotropic virus type I (HTLV-I) isolated from patients with HTLV-I-associated myelopathy or encephalopathy.
Two cell lines, CNS-5 and CNS-6, were established by cocultivation of sedimented cells in cerebrospinal fluid (CSF) from two anti-human T-lymphotropic virus type I (HTLV-I) antibody-positive male patients with encephalopathy and HTLV-I-associated myelopathy, respectively, with peripheral blood mononuclear cells from a healthy seronegative female. These cell lines, possessing a normal female karyotype, revealed similar characteristics as follows; they expressed HTLV-I-related antigens, they produced C-type retrovirus particles, HTLV-I provirus genomes were integrated into their DNAs, and they had CD4+ activated T-cell markers. In addition, immunocytochemical and immunoelectron microscopic studies showed peculiar immunoreactivity of these cell lines with anti-alpha/beta T-cell antigen receptor (TCR) antibodies; beta Fl, defining beta chain epitope, was only positive in the perinuclear spaces and rough endoplasmic reticulum in some cells, and WT31, recognizing alpha/beta framework, was mostly negative, while CD3 was expressed in the majority of the cells. These facts indicate that HTLV-I-infected cells were present in CSF of these two patients, and suggest that neurological disorders associated with HTLV-I may not be restricted to myelopathy and may include brain abnormalities.
Iwata
J
J
Department of Pathology, Kochi Medical School, Japan.
Oka
T
T
Furihata
M
M
Sonobe
H
H
Matsubayashi
K
K
Uemura
Y
Y
Miyoshi
I
I
Ohtsuki
Y
Y
D000368:Aged; D000956:Antigens, Viral / Q000032:analysis; D015139:Blotting, Southern; D001927:Brain Diseases / Q000382:microbiology*; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology*; D002460:Cell Line / Q000382:microbiology*; D015490:HTLV-I Infections / Q000382:microbiology*; D015368:Human T-lymphotropic virus 1 / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D015493:Paraparesis, Tropical Spastic / Q000382:microbiology*
D000956:Antigens, Viral