DNA restriction fingerprint analysis of the soil bacterium Azospirillum.
Total DNAs of 18 strains of Azospirillum from different sources and geographical areas were compared by restriction endonuclease pattern analysis. Fragments obtained with HindIII or BglII were separated by PAGE and stained with silver nitrate. Each strain possessed a unique and reproducible fingerprint with each enzyme, thereby facilitating strain recognition. UPGMA analysis recovered clusters of band patterns that were compared to the distribution of species within the genus Azospirillum.
Istituto di Microbiologia Agraria e Tecnica, University of Florence, Italy.
L
L
Giovannetti
S
S
Ventura
M
M
Bazzicalupo
R
R
Fani
R
R
Materassi
D001419:Bacteria / Q000235:genetics*; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D009710:Nucleotide Mapping; D015183:Restriction Mapping; D012834:Silver; D013194:Staining and Labeling
D004269:DNA, Bacterial; D012834:Silver