Distribution of human granulocyte colony-stimulating factor receptors on hematopoietic and nonhematopoietic tumor cell lines.
Introduction of two tyrosine residues into human granulocyte colony-stimulating factor (G-CSF) allowed us to obtain radioiodinated material efficiently. By using this material, specific receptors for human G-CSF were analyzed. It was found that human G-CSF receptors were expressed on several myelocytic and monocytic leukemia cell lines. Furthermore, they were also expressed on two choriocarcinoma cell lines. However, the human G-CSF receptors were not detected on other nonhematopoietic tumor cell lines examined, indicating that expression of G-CSF receptors is strictly limited as compared with receptors for granulocyte/macrophage colony-stimulating factor (GM-CSF), which are widely distributed on nonhematopoietic tumors.
Kuwaki
T
T
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo.
Hosoi
T
T
Hanazono
Y
Y
Tsumura
H
H
Ishikawa
F
F
Miyazono
K
K
Miyagawa
K
K
Takaku
F
F
D002460:Cell Line; D002822:Choriocarcinoma / Q000378:metabolism*; D003115:Colony-Stimulating Factors / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D016188:Receptors, Granulocyte Colony-Stimulating Factor
D003115:Colony-Stimulating Factors; D011956:Receptors, Cell Surface; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D016179:Granulocyte Colony-Stimulating Factor