[The enhancement of the penetration of the human immunodeficiency virus into cells with the aid of a helper virus].
Penetration of human immunodeficiency virus (HIV) into the cells of lymphoblastoid T-cell line H9 was studied using 35S-methionine-labeled virus by demonstration of virus-specific proteins in the cytoplasm of the infected cells. Purification of the virus by ultracentrifugation through 30% glycerol was shown to lead to virus aggregation and its partial destruction manifested by the loss of gp120 protein, therefore unlabeled concentrated virus was used mainly with subsequent determination of virus-specific proteins by immune blotting. The addition of Sendai virus inactivated with UV rays to HIV increased the amount of HIV associated with cells as well as the amount of virus-specific proteins in the cytoplasm of the infected cells.
Sharova
N K
NK
Bukrinskaia
A G
AG
D002478:Cells, Cultured / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D015497:HIV-1 / Q000302:isolation & purification / Q000472:pathogenicity*; D006378:Helper Viruses / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D015151:Immunoblotting; D008715:Methionine; D010222:Parainfluenza Virus 1, Human / Q000302:isolation & purification / Q000472:pathogenicity / Q000528:radiation effects; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D013462:Sulfur Radioisotopes; D013601:T-Lymphocytes / Q000382:microbiology; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000032:analysis; D014775:Virus Activation / Q000528:radiation effects
D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D008715:Methionine; D012194:RNA-Directed DNA Polymerase
Usilenie proniknoveniia virusa immunodefitsita cheloveka v kletki s pomoshch'iu virusa-pomoshchnika.