Linkage mapping of highly informative DNA polymorphisms within the human interferon-alpha receptor gene on chromosome 21.
Two polymorphic loci within the interferon-alpha receptor (IFNAR) gene on human chromosome 21 have been identified and mapped by linkage analysis in 40 CEPH families. These markers are (1) a multiallelic RFLP with an observed heterozygosity of 0.72 and (2) a variable (AT3)n short sequence repeat at the poly(A) tail of an Alu sequence (AluVpA) with an observed heterozygosity of 0.83. This locus is close to D21S58 (theta = 0.02, zeta = 36.76) and D21S17 (theta = 0.02, Zeta = 21.76) with chromosomal band 21q22.1. Multipoint linkage analysis suggests the most likely locus order to be 21cen-D21S58-IFNAR-D21S17-21qter. Given its high heterozygosity, the IFNAR gene can be used as an index marker on human chromosome 21.
McInnis
M G
MG
Center for Medical Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Lutfalla
G
G
Slaugenhaupt
S
S
Petersen
M B
MB
Uze
G
G
Chakravarti
A
A
Antonarakis
S E
SE
D001483:Base Sequence; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D005260:Female; D008040:Genetic Linkage / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism*; D007438:Introns / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D011971:Receptors, Immunologic / Q000235:genetics*; D017471:Receptors, Interferon; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D005819:Genetic Markers; D016898:Interferon-alpha; D011971:Receptors, Immunologic; D017471:Receptors, Interferon