Corticotropin releasing hormone and arginine vasopressin stimulation of ACTH and substance P in human mononuclear leukocytes.
Bacterial lipopolysaccharide (LPS) and corticotropin releasing hormone (CRH) plus arginine vasopressin (AVP) induce immunoassayable (1-13)ACTH (alpha MSH) from mononuclear leukocytes. We studied the ability of LPS and CRH + AVP to in vitro stimulate native ACTH (not alpha MSH) and substance P (SP) production and thymidine incorporation in human mononuclear leukocytes. Neither CRH + AVP nor LPS stimulated detectable amounts of intracellular or extracellular ACTH (less than 15 pg/8 x 10(6) cells or total medium) or SP (less than 50 pg/8 x 10(6) cells or total medium) at 1, 2, 3 or 4 days of incubation. LPS, but not CRF + AVP, increased the amount of 3H-thymidine incorporation over controls. This data questions the importance of an immunoadrenal axis and the synthesis of SP by mononuclear leukocytes.
Sobel
D O
DO
Georgetown University Medical School, Washington, DC 20007.
D000328:Adult; D001127:Arginine Vasopressin / Q000494:pharmacology*; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D010446:Peptide Fragments / Q000096:biosynthesis*; D013373:Substance P / Q000096:biosynthesis*; D013745:Tetanus Toxoid / Q000494:pharmacology; D000521:alpha-MSH / Q000031:analogs & derivatives* / Q000096:biosynthesis
D008070:Lipopolysaccharides; D010446:Peptide Fragments; D013745:Tetanus Toxoid; D001127:Arginine Vasopressin; D013373:Substance P; D000521:alpha-MSH; D004247:DNA; D003346:Corticotropin-Releasing Hormone