[Loss of Ha-ras alleles in DNA from gastric carcinoma tissue of Chinese individuals].
In this study, a human c-Ha-ras-1 6 kilobase recombinant clone (EcoRI fragment) was used as a probe, DNA from 13 gastric carcinoma patients heterozygous for the Ha-ras locus contained two Ha-ras-related BamHI restriction fragments. Southern blotting results showed similar patterns of allelic restriction fragments between gastric carcinoma and normal gastric tissue DNA in 12 cases. But one heterozygous patient (6.3kb/7.8kb) lost a 6.3kb allelic restriction fragment in the gastric carcinoma tissue. We also examined the RFLPs of the Ha-ras oncogene in one pedigree of a heterozygous gastric carcinoma patient. The results showed that one daughter had lost one Ha-ras allele in WBC DNA. This Ha-ras allele loss may have resulted from a mitotic nondisjunction or a mitotic recombination event.
Nanjing Railway Medical College.
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Shan
D000483:Alleles; D015139:Blotting, Southern; D002872:Chromosome Deletion; D011905:Genes, ras; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D013274:Stomach Neoplasms / Q000235:genetics*