Inhibition of potassium-induced release of histamine from mast cells by tetraethylammonium and tetramethylammonium.
In accordance with our previous results, a marked release of histamine (HA) from rat peritoneal mast cells was initiated by 150 mM KCl in the absence of extracellular Ca2+. This release could be reduced by 20-60 mM tetraethylammonium (TEA) or tetramethylammonium (TMA), the non-selective K(+)-channel blockers, Ouabain, the general inhibitor of (Na+ + K+) ATP-ase, failed to produce any changes in this release. The action of TEA discriminated between the initiation of HA release evoked by different agents, producing a blockade of the K(+)-induced but not the 48/80-stimulated HA release. In total, these data suggest the presence of TEA/TMA-sensitive K(+)-channels in the mast cell membrane and their involvement in one of the possible pathways for the initiation of HA release.
Department of Anatomy II, Semmelweis Medical School, Budapest, Hungary.
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Németh
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Magyar
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Huszti
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D013757:Tetraethylammonium Compounds / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D015221:Potassium Channels; D000644:Quaternary Ammonium Compounds; D013757:Tetraethylammonium Compounds; D003189:p-Methoxy-N-methylphenethylamine; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; C027917:tetramethylammonium; D011188:Potassium