Transforming growth factor-beta 1 stimulates fibronectin production in bovine adrenocortical cells in culture.
Transforming growth factor-beta (TGF-beta 1) suppresses cortisol production when added to cultured bovine adrenocortical (BAC) cells while concomitantly increasing fibronectin synthesis and assembly into extracellular fibrils. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of gelatin-Sepharose-treated media from BAC cells demonstrated a 2-fold stimulation of fibronectin production by TGF-beta 1 in both the presence and absence of serum. Indirect immunofluorescence studies revealed that TGF-beta 1 caused a striking increase in the fibronectin content of BAC extracellular matrix. TGF-beta 1 caused a 4-fold increase in deoxycholate-insoluble fibronectin after 12 h and a 7-fold increase after 24 h over that of control BAC cultures. Northern hybridization analyses indicated that TGF-beta 1 stimulated levels of fibronectin poly(A)+ RNA 2.3-fold. We found that cultured BAC cells express TGF-beta 1 mRNA, suggesting a possible autocrine role for TGF-beta 1 in the adrenal.
Environmental Toxicology Center, University of Wisconsin, Madison 53706.
C A
CA
Williams
B L
BL
Allen-Hoffmann
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001792:Blood Platelets / Q000502:physiology; D015152:Blotting, Northern; D002417:Cattle; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D008970:Molecular Weight; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D015291:Transforming Growth Factors / Q000097:blood / Q000302:isolation & purification / Q000494:pharmacology*
D005353:Fibronectins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D015291:Transforming Growth Factors