In vitro infection of monocytes with HIVBa-L. Effect on cell surface expression of CD4, CD14, HLA-DR, and HLA-DQ.
Monocytes from healthy blood donors were inoculated in vitro with a monocyte-tropic strain of human immunodeficiency virus type 1 HIVBa-L. HIV replication was first detected at Day 5 postinoculation, with peak virus activity at Day 17. We assessed the kinetics of the expression of four monocyte surface antigens (CD14, CD4, HLA-DR, and HLA-DQ) on HIV-infected and uninfected monocyte/macrophages, (M phi) by flow cytometry. We consistently found a decreased expression of CD4 and CD14 on HIV-infected M phi compared to their expression on M phi of uninfected controls. In contrast, HLA-DR and HLA-DQ expression was unchanged on HIV-infected M phi.
Department of Pathology, Emory University School of Medicine, Atlanta, Georgia 30322.
L M
LM
Melendez-Guerrero
J K
JK
Nicholson
J S
JS
McDougal
D015214:Antigens, Differentiation, Myelomonocytic; D000954:Antigens, Surface; D015704:CD4 Antigens; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D006801:Humans; D066298:In Vitro Techniques; D018950:Lipopolysaccharide Receptors; D009000:Monocytes / Q000276:immunology / Q000382:microbiology*; D014779:Virus Replication
D015214:Antigens, Differentiation, Myelomonocytic; D000954:Antigens, Surface; D015704:CD4 Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D018950:Lipopolysaccharide Receptors