Molecular cloning of the murine BP-1/6C3 antigen: a member of the zinc-dependent metallopeptidase family.
The BP-1/6C3 antigen is a phosphorylated cell surface glycoprotein that can be identified by monoclonal antibodies on mouse pre-B cells, immature B cells, and certain stromal cell lines from bone marrow. Expression of this antigen is increased in stromal-dependent pre-B cell lines and retrovirally transformed pre-B cells. Expression of the BP-1/6C3 antigen thus correlates with proliferation and transformation of immature B-lineage cells. In this study, we report the isolation and characterization of cDNAs encoding the BP-1/6C3 antigen. Northern blot analysis revealed a major 4.1-kilobase mRNA in all BP-1/6C3+ mouse pre-B lines and in a human pre-B cell line. BP-1/6C3 mRNA was either absent or truncated in BP-1/6C3- cell lines. The cDNA sequence predicts a type II integral membrane protein of 945 amino acids with an intracytoplasmic amino terminus of only 17 amino acids and a typical zinc-binding motif in its extracellular domain. BP-1/6C3 has significant homology to aminopeptidase N and is the second member of the zinc-dependent metallopeptidase gene family to be found on the surface of early B-lineage cells.
Wu
Q
Q
Division of Developmental and Clinical Immunology, University of Alabama, Birmingham 35294.
Lahti
J M
JM
Air
G M
GM
Burrows
P D
PD
Cooper
M D
MD
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D015032:Zinc
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; C062906:BP-1-6C3 antigen; D008666:Metalloendopeptidases; D015032:Zinc