Induction of severe experimental autoimmune neuritis with a synthetic peptide corresponding to the 53-78 amino acid sequence of the myelin P2 protein.
We generated a synthetic peptide (SP-26), corresponding to the amino acid residues 53-78 of bovine P2 protein, which induced severe clinical and pathological characteristics of experimental autoimmune neuritis (EAN) in Lewis rats. Lymph node cell populations from SP-26-immunized rats elicited a proliferative response to the peptide and to the P2 protein. After 16 cycles of antigen stimulation with the peptide, the SP-26 T cell line shows a decreased response to P2, but not to SP-26. Fluorescence-activated cell sorter (FACS) analysis of a SP-26 T cell line indicated the majority of cells to be of CD4+ CD8-. This report demonstrates that the synthetic peptide SP-26 can induce severe EAN in Lewis rats in a dose-dependent manner. Furthermore, specific T cell lines reactive to SP-26 can be generated from the lymph nodes of SP-26-immunized rats.
Department of Neurology, Hospital of The University of Pennsylvania, Philadelphia 19104.
A
A
Rostami
S K
SK
Gregorian
M J
MJ
Brown
D E
DE
Pleasure
D000818:Animals; D002460:Cell Line; D005260:Female; D008198:Lymph Nodes; D004676:Myelin Basic Protein / Q000009:adverse effects* / Q000276:immunology; D018996:Myelin P2 Protein; D009444:Neuritis, Autoimmune, Experimental / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D010446:Peptide Fragments; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology
D004676:Myelin Basic Protein; D018996:Myelin P2 Protein; D010446:Peptide Fragments