Characterization of glucoamylase from Aspergillus terreus 4.
A strain of Aspergillus terreus 4 was found to show extracellular amylolytic activity and the amylase was identified as glucoamylase enzyme. The optimum temperature for the enzyme activity was 60% and it was stable at this temperature for 1 h. The enzyme was optimally active at pH 5.0 and stable between pH 3.0-8.0. Km values of glucoamylase for soluble starch, amylose and amylopectin were 5.9 mg/ml, 4.8 mg/ml and 2.6 mg/ml respectively.
Department of Microbiology, Bose Institute, Calcutta, India.
A
A
Ghose
B S
BS
Chatterjee
A
A
Das
D001230:Aspergillus / Q000201:enzymology*; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007455:Iodine; D007700:Kinetics; D013194:Staining and Labeling; D013213:Starch; D013379:Substrate Specificity; D013696:Temperature
D013213:Starch; D007455:Iodine; D005087:Glucan 1,4-alpha-Glucosidase