Identification of endothelial and mesothelial cells in human omental tissue and in omentum-derived cultured cells by specific cell markers.
Human omental tissue has been used as a source for the isolation and cultivation of microvascular endothelial cells, but also for mesothelial cells. Since both cell types have several morphologic and functional features in common, concerns were raised whether endothelial cells can be separated from mesothelial cells by the methods described for the isolation of microvascular endothelial cells. In the present study, endothelial cells were identified in the capillaries of native human omentum by several endothelial-cell specific markers. von Willebrand factor was demonstrated by polyclonal and monoclonal antibodies, a lectin-specific ligand by Ulex europaeus I, and an endothelial-cell specific surface epitope by the monoclonal antibody, PAL-E. These markers were not found positive with mesothelial cells of native omentum. Mesothelial cells were identified by monoclonal antibodies against the intermediate filaments, cytokeratin and vimentin. After having demonstrated the specificity of the methods for the distinction between endothelial and mesothelial cells within native omentum, these methods were applied to omentum-derived cells previously claimed to be microvascular endothelial cells. These cultured cells proved to be negative for von Willebrand factor, Ulex europaeus I ligand and PAL-E epitope. In contrast to this, the cultivated cells stained positive to cytokeratin and vimentin. Furthermore, it was shown by immunoprecipitation studies that omentum-derived cells did not synthesize and secrete vWF, indicating the nonendothelial nature of these cells. Finally, electron microscopy demonstrated microvilli on the surface of cultivated omentum-derived cells indicative for the mesothelial origin of these cells. The data presented demonstrate that the cells obtained using the previously published methods for the isolation and cultivation of "microvascular endothelial cells" from omental tissue are of mesothelial and not of endothelial origin. Thus, a great number of data obtained with this type of omentum-derived cells thought to be microvascular endothelial cells need re-evaluation.
Pötzsch
B
B
Clinical Research Unit for Blood Coagulation and Thrombosis, Max-Planck-Gesellschaft, Giessen, West Germany.
Grulich-Henn
J
J
Rössing
R
R
Wille
D
D
Müller-Berghaus
G
G
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology; D004847:Epithelial Cells; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008648:Mesoderm / Q000166:cytology*; D009852:Omentum; D014746:Vimentin / Q000032:analysis; D014841:von Willebrand Factor / Q000032:analysis
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D014746:Vimentin; D014841:von Willebrand Factor; D007633:Keratins