Loss of immunorecessive cytotoxic T lymphocyte determinant V on SV40 T antigen following cocultivation with site-specific cytotoxic T lymphocyte clone Y-5.
Five distinct antigenic sites recognized by cytotoxic T lymphocytes in H-2b mice have been identified on simian virus 40 (SV40) T antigen. Cocultivation of SV40-transformed cells with cytotoxic T lymphocyte (CTL) clones specific for epitopes I-IV results in the selection of variants which show a loss of either epitopes I, II, and III, II and III, or IV. We now report the isolation of a variant SV40-transformed cell line which has lost the expression of all five CTL epitopes on SV40 T antigen. These variant cells were no longer susceptible to lysis by CTL clones specific for all five epitopes. These variant cells would be used to relate alterations in SV40 T antigen structure with functional changes in the transformed cells.
Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey 17033.
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Tanaka
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Tevethia
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D002461:Cell Line, Transformed; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D013539:Simian virus 40 / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000957:Antigens, Viral, Tumor; D000939:Epitopes; D015395:Histocompatibility Antigens Class I