The effect of diterpenoidic diacylglycerols on tentacle regeneration in Hydra vulgaris.
1. The effect of two ichthyotoxic diterpenoid diacylglycerols, verrucosins A and B, previously isolated from the mantle of a marine nudibranch mollusc, were studied on Hydra vulgaris tentacle regeneration. 2. A potent effect was found for both verrucosins in the low nanomolar range; the rank of potency observed was analogous to that reported for diglycerid activation of protein kinase C and to that found for verrucosin activation of this enzyme. 3. In the high nanomolar range, the two verrucosins were found to be toxic to Hydra vulgaris. 4. Verrucosin B-induced toxicity and tentacle regeneration were found to be dependent on [Ca2+] in the assay medium.
Istituto di Cibernetica del Consiglio Nazionale delle Ricerche, Arco Felice, Italy.
L
L
De Petrocellis
V
V
Di Marzo
B
B
Arcà
M
M
Gavagnin
R
R
Minei
G
G
Cimino
D000818:Animals; D004075:Diglycerides / Q000494:pharmacology*; D004224:Diterpenes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006829:Hydra / Q000187:drug effects*; D012038:Regeneration / Q000187:drug effects*
D004075:Diglycerides; D004224:Diterpenes; C074567:verrucosin B; C074568:verrucosin A