Antigenic and biochemical characterization of bovine rotavirus V1005, a new member of rotavirus serotype 10.
Bovine rotavirus (BRV) V1005 is serologically distinct from rotavirus serotypes 1, 2, 3, 4, 5, 6, 8 and 9. BRV V1005 showed cross-reactions with BRV B223, the American prototype of serotype 10 rotavirus, and with BRV E4049, a British serotype 10 isolate. BRV V1005 was, however, not neutralized by four monoclonal antibodies directed against VP7 of BRV B223. Two-way cross-reactions were observed between BRV V1005 and a reassortant rotavirus containing the VP4 from BRV UK. In addition the major tryptic cleavage product of VP4, VP5*, from BRV V1005 is indistinguishable by peptide mapping and its isoelectric point from the homologous protein of BRV UK, but is clearly different from VP5* of BRV NCDV. The peptide map of VP7 from BRV V1005 differed from that obtained for VP7 of BRV UK.
Nestlé Research Centre, Nestec Ltd, Lausanne, Switzerland.
H
H
Brüssow
D
D
Snodgrass
T
T
Fitzgerald
W
W
Eichhorn
R
R
Gerhards
A
A
Bruttin
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle / Q000382:microbiology*; D002478:Cells, Cultured; D003429:Cross Reactions; D000939:Epitopes; D006168:Guinea Pigs; D007525:Isoelectric Focusing; D009500:Neutralization Tests; D010449:Peptide Mapping; D011817:Rabbits; D012401:Rotavirus / Q000145:classification / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D014763:Viral Matrix Proteins