Expression of bovine leukaemia virus antigens fused to MS2 polymerase in E. coli.
The main core protein and segments of envelope proteins of bovine leukaemia virus fused to MS2 polymerase were expressed in E. coli. The synthesis rate varied between 3 and 25% of the total cellular proteins. BLV-MS2 polymerase fusion proteins were detected immunologically using rabbit anti-BLV sera, monoclonal antibodies and a serum of a BLV-infected cow.
Dept. Medicine (Charité), Humboldt University, Berlin, Germany.
R
R
Ulrich
S
S
Bähring
H
H
Siakkou
S
S
Rosenthal
D000956:Antigens, Viral / Q000096:biosynthesis* / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D015686:Gene Products, env / Q000096:biosynthesis / Q000235:genetics; D015683:Gene Products, gag / Q000096:biosynthesis / Q000235:genetics; D015752:Genes, env; D015750:Genes, gag; D005822:Genetic Vectors; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*
D000956:Antigens, Viral; D015686:Gene Products, env; D015683:Gene Products, gag; D011993:Recombinant Fusion Proteins; C073587:MS2 polymerase; D012321:DNA-Directed RNA Polymerases