Rearrangement and expression of T-cell receptor genes in marmoset lymphoid cell lines with natural killer activity.
To study the role of the T-3/T-cell receptor complex in mediating natural killer activity, we have examined T-cell receptor gene configuration and expression in five transformed marmoset cell lines with natural killer activity. All lines showed rearrangement of the beta chain of the T-cell receptor and expressed full size beta chain transcripts as well as full size delta chain transcripts. Only three lines contained full size T-cell receptor alpha chain transcripts. Of the three lines which had potential for encoding functional T-3/T-cell receptor complex, those which possessed natural killer activity also expressed T-gamma transcripts. In addition, one clone with high natural killer activity which did express high levels of T-gamma mRNA was unable to produce functional T-3/T-cell receptor complex due to truncated T-cell receptor alpha chain message. The results in this paper provide strong evidence that natural killer activity is dependent upon expression of the T-cell receptor gamma chain.
Stevenson
M
M
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha.
Johnson
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DR
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002143:Callitrichinae; D002460:Cell Line; D015329:Gene Rearrangement, T-Lymphocyte / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D012313:RNA / Q000096:biosynthesis; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta
D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D012313:RNA