Homology of mecA gene in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans to that of Staphylococcus aureus.
A penicillin-binding protein of molecular weight 76,000 inducible by beta-lactams was detected in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans. DNA from these strains hybridized to the mecA gene from Staphylococcus aureus; however, the chromosomal HindIII fragments containing the mecA genes were 3.4 kilobases in S. haemolyticus and 4.3 kilobases in S. simulans.
Department of Clinical Pathology, Teikyo University School of Medicine, Tokyo, Japan.
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K
Ubukata
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Nonoguchi
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MD
Song
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Matsuhashi
M
M
Konno
D001426:Bacterial Proteins; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000032:analysis / Q000302:isolation & purification; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006602:Hexosyltransferases; D008712:Methicillin / Q000494:pharmacology*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000032:analysis / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010403:Penicillin Resistance; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D015183:Restriction Mapping; D013194:Staining and Labeling; D013210:Staphylococcus / Q000187:drug effects* / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D015247:Deoxyribonuclease HindIII; D002267:Muramoylpentapeptide Carboxypeptidase; D008712:Methicillin