Simplified polyacrylamide gel electrophoresis and silver staining for analysis and preparation of influenza virus RNA segments.
The influenza virus has a genome consisting of eight RNA segments. A simplified technique to study the RNA segmental pattern by silver staining after gel electrophoresis has been developed. In addition, individual RNA segments could be isolated by a combination of polyacrylamide gel electrophoresis and isotachophoresis.
Abusugra
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Swedish University of Agricultural Sciences, Faculty of Veterinary Medicine, Uppsala.
Linné
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Berg
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D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D014644:Genetic Variation; D009975:Orthomyxoviridae / Q000235:genetics*; D012367:RNA, Viral / Q000302:isolation & purification*; D012834:Silver; D013194:Staining and Labeling
D012367:RNA, Viral; D012834:Silver