Thy-1-specific helper T cells can provide intramolecular help, but can provide intermolecular help only if the phosphatidylinositol anchor of Thy-1 is replaced by a membrane insert.
We have investigated the ability of Thy-1 to serve as a helper antigen in delivering intermolecular help. The parental Thy-1 molecule, which is attached to the cell surface via a phosphatidylinositol anchor, failed to deliver intermolecular help for an antibody response to H-2 alloantigens. This failure appears to be due to the phosphatidylinositol anchor since a Thy-1 hybrid construct, which is expressed as a transmembrane cell surface molecule, can serve as helper antigen and deliver intermolecular help.
Department of Biology, University College London, Great Britain.
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Stauss
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Mitchison
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Yao
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D007116:Immunization, Passive; D007739:L Cells; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D010716:Phosphatidylinositols / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D018800:Thy-1 Antigens; D014162:Transfection
D000954:Antigens, Surface; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins; D010716:Phosphatidylinositols; D011993:Recombinant Fusion Proteins; D018800:Thy-1 Antigens