Freeze-fracture and immunomorphological analysis of spiral ganglion cells.
Freeze-fracture analysis of adult spiral ganglion cells of CBA/CBA mice revealed two types of membrane specializations. Most cells (type I) had a smooth surface and were surrounded by Schwann cells. Type II spiral ganglion cells showed numerous membrane specializations with well-delineated indentations similar to those previously found on hair cells adjacent to afferent and efferent nerve endings. Immunomorphological analysis (using well-defined monoclonal antibodies directed against different subclasses of intermediate filament proteins) revealed a unique co-expression of neurofilaments, vimentin and cytokeratins in spiral ganglion cells of 8- to 22-week human fetuses.
Department of Oto-Rhino-Laryngology and Head and Neck Surgery, University of Umeå, Sweden.
M
M
Anniko
L E
LE
Thornell
I
I
Virtanen
F C
FC
Ramaekers
D000818:Animals; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000648:ultrastructure; D003051:Cochlea / Q000196:embryology / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure; D005614:Freeze Fracturing; D006801:Humans; D007633:Keratins / Q000378:metabolism*; D051379:Mice; D008808:Mice, Inbred CBA; D012583:Schwann Cells / Q000648:ultrastructure; D013136:Spiral Ganglion / Q000196:embryology / Q000648:ultrastructure*; D014746:Vimentin / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D014746:Vimentin; D007633:Keratins