Background-protein effects on fluorophotometric data.
Fluorescent tracers are commonly used in fluorophotometric studies of ocular fluids and tissues that contain background protein. Background-protein concentrations were found to decrease or increase significantly the measure of fluorescence emitted from solutions containing sodium fluorescein, fluorescein-labeled dextran, or fluorescein-labeled horseradish peroxidase. The effect of background protein on fluorescence was expressed as a function of the specific fluorescent tracer, tracer concentration, and background-protein concentration; it can be corrected in the analysis of fluorophotometric data. Fluorophotometric studies--particularly those in which the background-protein level is expected to be abnormally high, such as postoperative and pathologic studies--may need to include either a data correction based on measured effects of background protein on tracer fluorescence or, in the case of clinical investigations, recognize at least the potential for a range of possible interpretations.
Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge.
M F
MF
Barsotti
S P
SP
Bartels
R D
RD
Kamm
T F
TF
Freddo
D003911:Dextrans; D019793:Fluorescein; D005452:Fluoresceins; D005453:Fluorescence; D005456:Fluorescent Dyes; D015590:Fluorophotometry; D006735:Horseradish Peroxidase; D011506:Proteins / Q000378:metabolism*; D012969:Sodium Fluoride
D003911:Dextrans; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011506:Proteins; C052526:fluorescein-dextran; D012969:Sodium Fluoride; D006735:Horseradish Peroxidase; D019793:Fluorescein