Presentation of Leishmania donovani promastigotes occurs via a brefeldin A-sensitive pathway.
For the presentation of Leishmania promastigotes to polyclonal CD4+ T cells, a processing period within activated macrophages of 3-4 h is required. Presentation can be inhibited by both chloroquine and brefeldin A (BFA), the latter implicating a requirement for newly synthesized MHC class II molecules. This inhibition is both reversible and specific, in that BFA did not inhibit mixed lymphocyte reaction stimulation by these infected macrophages. Immunogold labeling demonstrated that class II was associated with the parasite-containing phagolysosome. The level of class II was not significantly altered in BFA-treated cells in the time period studied, suggesting that antigen may exist the phagolysosome and interact with class II in another cellular compartment.
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine.
T
T
Lang
P M
PM
Kaye
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000953:Antigens, Protozoan / Q000378:metabolism*; D020126:Brefeldin A; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002738:Chloroquine / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007893:Leishmania donovani / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology
D000953:Antigens, Protozoan; D003517:Cyclopentanes; D000949:Histocompatibility Antigens Class II; D020126:Brefeldin A; D002738:Chloroquine