Sulfonylurea enhances insulin-induced acetyl coenzyme A carboxylase activity in rat adipocytes.
The effect of sulfonylurea on the activity of acetyl-coenzyme A carboxylase, a rate limiting enzyme of lipogenesis, was investigated using isolated rat adipocytes. Insulin significantly increased the enzyme activity by 170% of the control level, while glucagon and epinephrine decreased the activity of the enzyme by 53% and 64% of the control, respectively. In the presence of tolbutamide (10(-3) M) or glibenclamide (10(-6) M), a significant potentiation of insulin action was found in adipocytes. In addition, sulfonylurea restored the activity of acetyl-CoA carboxylase reduced by glucagon or epinephrine to the control level. Sulfonylurea enhancement of the acetyl-CoA carboxylase activity may offer one possible explanation for a mechanism of antilipolytic action of the drug in adipocytes.
Third Department of Internal Medicine, Yamaguchi University School of Medicine, Ube, Japan.
M
M
Matsuda
K
K
Kaku
M
M
Aoki
H
H
Inoue
T
T
Kaneko
D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis*; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D004790:Enzyme Induction / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D005934:Glucagon / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013453:Sulfonylurea Compounds / Q000494:pharmacology*; D014044:Tolbutamide / Q000494:pharmacology
D007328:Insulin; D013453:Sulfonylurea Compounds; D005934:Glucagon; D014044:Tolbutamide; D000103:Acetyl-CoA Carboxylase; D005905:Glyburide; D004837:Epinephrine