Specific expression of human endothelin-2 (ET-2) gene in a renal adenocarcinoma cell line. Molecular cloning of cDNA encoding the precursor of ET-2 and its characterization.
Analysis of culture medium of human renal adenocarcinoma cells ACHN by RP-HPLC suggested that the cells specifically secreted human endothelin-2 (ET-2). cDNAs encoding human ET-2 precursor were cloned from a cDNA library constructed with mRNA derived from the ACHN cells, and the nucleotide and deduced amino acid sequences were determined. The ET-2 cDNA was revealed to contain 1.3 kb and encode prepro-ET-2 protein consisting of 178 amino acid residues. The ET-like sequences found in the prepro-ET-1 and -ET-3 was conserved in this prepro-ET-2. The Northern blot analysis of mRNA suggested that the transcript of ET-2 gene was 1.4 kb. This is the first direct evidence that human ET-2 gene was expressed specifically in tumor cells.
Tsukuba Research Laboratories, Takeda Chemical Industries, Ibaraki, Japan.
S
S
Ohkubo
K
K
Ogi
M
M
Hosoya
H
H
Matsumoto
N
N
Suzuki
C
C
Kimura
H
H
Ondo
M
M
Fujino
D000230:Adenocarcinoma; D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D019332:Endothelin-1; D016232:Endothelins / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D006801:Humans; D007680:Kidney Neoplasms; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D011498:Protein Precursors / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping
D004273:DNA, Neoplasm; D019332:Endothelin-1; D016232:Endothelins; D015345:Oligonucleotide Probes; D011498:Protein Precursors; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA