Lowered responsiveness of bronchoalveolar lavage T lymphocytes in hypersensitivity pneumonitis.
We previously reported that Trichosporon cutaneum was the major causative antigen of summer-type hypersensitivity pneumonitis (HP) in Japan. In summer-type HP patients, we noticed that the proliferative responses of bronchoalveolar lavage (BAL) lymphocytes to phytohemagglutinin (PHA) and concanavalin A were significantly lower than those of the peripheral blood lymphocytes of the same patients. It was shown in this study that the low response of BAL lymphocytes was due to an intrinsic lowering of the responsiveness of the T cells. Results of the mixed culture experiments, in which the responses to mitogens of BAL and peripheral blood T cells mixed with either alveolar macrophages or blood monocytes were compared, indicated that the decreased proliferative response was due neither to the suppressive effect nor to defects in accessory function of the alveolar macrophages. BAL T cells did not act as suppressor cells when they were added to the culture of peripheral T cells. The decreased proliferative response was not due to the dominance of CD8+ T cells frequently seen in BAL cells of HP patients, because both CD4+ and CD8+ T cells separated from BAL cells of HP patients showed lower responsiveness than those of peripheral blood T cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Yamasaki
H
H
Department of Biochemistry, Kumamoto-University Medical School, Japan.
Kinoshita
T
T
Ohmura
T
T
Ando
M
M
Soda
K
K
Sakata
T
T
Araki
S
S
Onoue
K
K
D000328:Adult; D000542:Alveolitis, Extrinsic Allergic / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D015703:Antigens, CD / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology / Q000382:microbiology; D018821:CD18 Antigens; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D008213:Lymphocyte Activation; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000382:microbiology; D014250:Trichosporon / Q000276:immunology / Q000502:physiology*
D015703:Antigens, CD; D018821:CD18 Antigens; D007376:Interleukin-2; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D016028:Receptors, Leukocyte-Adhesion; D003208:Concanavalin A