Residue screening for the beta-agonists clenbuterol, salbutamol and cimaterol in urine using enzyme immunoassay and high-performance liquid chromatography.
The antibody for enzyme immunoassay was raised against clenbuterol-diazo-BSA, and salbutamol-carboxymethyl ether-biocytin was used as a label. Procedural blanks from 500 negative urine samples were always less than 0.2 ppb salbutamol or less than 0.02 ppb clenbuterol equivalents, and a residue level of 1 ppb was detected with good reliability. After treatment of veal calves with anabolic dosages, residue levels in urine amounted to 10-200 ppb clenbuterol or salbutamol. beta-Agonists were separated by high-performance liquid chromatography on LiChrospher RP-Select B columns, and acidic methanol-buffer or acetonitrile-buffer mobile phases. Combinations of high-performance liquid chromatography and enzyme immunoassay were used for confirmation.
Institut für Physiologie, Süddeutschen Versuchs- und Forschungsanstalt für Milchwirtschaft, Technische Universität München, Freising-Weihenstephan, Germany.
H H
HH
Meyer
L
L
Rinke
I
I
Dürsch
D000318:Adrenergic beta-Agonists / Q000652:urine*; D000420:Albuterol / Q000652:urine*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002976:Clenbuterol / Q000652:urine*; D004983:Ethanolamines / Q000652:urine*; D005260:Female; D007124:Immunoenzyme Techniques
D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; D000420:Albuterol; D002976:Clenbuterol; C047411:cimaterol