The genomic diversity and stability of field strains of suid herpesvirus 1 (Aujeszky's disease virus).
The genomic diversity among isolates of suid herpesvirus 1 (SHV-1) collected in the same herd and among clones from the same isolate was studied by restriction fragment pattern (RFP) analysis using BamHI. Tentatively defining a field strain as a transmissible entity, it was concluded that strains of SHV-1 commonly comprise distinguishable genomic variants. Contrary to the hypothesis of genomic lability, it is suggested that the pool of variants is sufficiently stable to specifically characterize a strain. The impact of the results on epizootiological methods based on identification of field isolates is discussed in connection with Aujeszky's disease in Denmark.
State Veterinary Institute for Virus Research, Kalvehave, Denmark.
L S
LS
Christensen
K J
KJ
Sørensen
D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003718:Denmark / Q000453:epidemiology; D015245:Deoxyribonuclease BamHI; D004196:Disease Outbreaks / Q000662:veterinary*; D014644:Genetic Variation; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D011557:Pseudorabies / Q000453:epidemiology / Q000382:microbiology*; D015183:Restriction Mapping; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*
D015245:Deoxyribonuclease BamHI