Expression of catfish amino acid taste receptors in Xenopus oocytes.
We demonstrate that poly (A+)RNA isolated from catfish barbels directs the expression of functional amino acid taste receptors in the Xenopus oocyte. The activity of these receptors is monitored in ovo by the two electrode voltage clamp technique. Specific conductance changes recorded in response to amino acid stimulation are analogous to those recorded electrophysiologically from intact catfish barbels. These responses exhibit specificity, reproducibility, rapid onset and termination, and desensitization to repetitive stimulation. A functional assay system that encompasses the full complement of transduction events from the ligand-receptor interaction to subsequent conductance changes is necessary to identify molecular components responsible for these events. Our results demonstrate that the Xenopus oocyte can be used to characterize and identify clones coding for amino acid taste receptors analogous to its use in studying receptor molecules for other neuroactive compounds.
Getchell
T V
TV
Department of Neuroscience, Roche Research Center, Nutley, N.J. 07110.
Grillo
M
M
Tate
S S
SS
Urade
R
R
Teeter
J
J
Margolis
F L
FL
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002397:Catfishes / Q000235:genetics / Q000502:physiology*; D004553:Electric Conductivity; D004594:Electrophysiology; D005071:Evoked Potentials; D005260:Female; D015870:Gene Expression; D008845:Microinjections; D009831:Olfactory Mucosa / Q000032:analysis; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D051381:Rats; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000235:genetics*; D012867:Skin / Q000032:analysis; D013649:Taste; D014982:Xenopus laevis
D000596:Amino Acids; D012333:RNA, Messenger; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D011061:Poly A; D012313:RNA