Experimental treatment of thioacetamide-induced liver cirrhosis by metenolone acetate. A morphological and biochemical study.
The influence of metenolone acetate (1 mg/kg b.m. orally) on intact and chronically thioacetamide-injured rat liver (experimental liver cirrhosis) was investigated over 14 d. Histological examination revealed nodular transformation of liver structure according to cirrhosis like lesions with hepatocellular and cholangiocellular proliferations. These structural alterations were more serious in the group treated with metenolone compared with the group without metenolone. Metanolone administration to animals with thioacetamide-induced experimental liver cirrhosis led to an increase in liver injury. This treatment seems to promote hepatic preneoplastic lesions induced by thioacetamide reflected by histology and induction of gamma-glutamyltranspeptidase and 7-ethoxycoumarin O-deethylase in injured livers. Metenolone did not interfere directly with the processes of connective tissue synthesis and degradation after thioacetamide pretreatment. Only little changes of the investigated biochemical parameters were seen after metenolone administration to animals with intact liver function: increases in serum cholinesterase and tissue N-acetyl-beta-D-glucosaminidase activity; decreases in N-acetyl-beta-D-glucosaminidase in serum, liver hydroxyproline content and hepatic gamma-glutamyltranspeptidase activity. The observed changes reflect hepatic adaption processes under the influence of metenolone. The results of this study indicate that the risk of anabolic steroids in adjuvant therapy of liver cirrhosis cannot be calculated at present.
Institute of Pharmacology and Toxicology, Friedrich Schiller University Jena F.R.G.
M
M
Kretzschmar
G
G
Machnik
A
A
Müller
F K
FK
Splinter
T
T
Zimmermann
W
W
Klinger
D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism; D000818:Animals; D001711:Biotransformation; D003238:Connective Tissue / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000503:physiopathology; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000188:drug therapy*; D008710:Methenolone / Q000031:analogs & derivatives* / Q000627:therapeutic use; D051381:Rats; D013853:Thioacetamide; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D013853:Thioacetamide; D008710:Methenolone; D015660:7-Alkoxycoumarin O-Dealkylase; D005723:gamma-Glutamyltransferase; D005978:Glutathione; C033244:methenolone acetate