[Cloning of Brucella genes in Escherichia coli K12 cells and analysis of products of the cloned genes].
The libraries of Brucella melitensis 565 and Brucella abortus 99 in Escherichia coli cells have been constructed. Some clones of Escherichia coli producing the specific brucella antigens have been found in immunological tests with brucella antiserum. Two strains producing antigens have been characterized, one being from Brucella melitensis 565 and another from Brucella abortus 99 clone libraries . Both strains synthesize two antigens that were studied by immunoelectrophoresis, immunoblotting after treatment of antigen preparations with different physical and chemical agents substrate specific enzymes. Both strains are found to synthesize the specific brucella antigens of protein nature. One of them has the mol mass about 15 kD, another--31-32 kD. The 31-32 kD antigen can be, evidently, referred to as the main protein of an outer membrane of brucella.
Gorelov
V N
VN
Kulakov
Iu K
IuK
Seliutina
D F
DF
Tokareva
L E
LE
Chibisova
V A
VA
Dranovskaia
E A
EA
Skavronskaia
A G
AG
D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry*; D015153:Blotting, Western; D002002:Brucella / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D004586:Electrophoresis; D000939:Epitopes / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008970:Molecular Weight
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000939:Epitopes
Klonirovanie genov Brucella v kletkakh Escherichia coli K12 i analiz produktov klonirovannykh genov.