Expression of rat pancreatic lipase gene is modulated by a lipid-rich diet at a transcriptional level.
The amount of cytoplasmic mRNAs specific for pancreatic lipase and amylase as well as transcription of the corresponding genes were investigated in rats fed a diet containing 25% sunflower oil. Concentration of lipase mRNA was actually increased by the lipid-rich carbohydrate-low diet and reached a maximum level after 2 days, but further remained constant for at least 10 days. In contrast, about a two-fold decrease in the concentration of pancreatic amylase mRNA was only observed after rats were fed the high-lipid diet for 10 days. Transcription rate measurement on isolated nuclei from pancreatic tissue indicated that the concentration of nascent lipase and amylase transcripts was consistent with a transcriptional regulation of expression of these two genes.
Centre de Biochimie et de Biologie Moléculaire du Centre National de la Recherche Scientifique, Marseille, France.
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Wicker
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Puigserver
D000681:Amylases / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004041:Dietary Fats / Q000494:pharmacology*; D015870:Gene Expression; D005796:Genes; D008049:Lipase / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014158:Transcription, Genetic / Q000187:drug effects*
D004041:Dietary Fats; D004247:DNA; D008049:Lipase; D000681:Amylases