Expression of T cell antigen receptor heterodimers in a lipid-linked form.
The interaction of the T cell receptor for antigen (TCR) with its antigen-major histocompatibility complex ligand is difficult to study because both are cell surface multimers. The TCR consists of two chains (alpha and beta) that are complexed to the five or more nonpolymorphic CD3 polypeptides. A soluble form of the TCR was engineered by replacing the carboxyl termini of alpha and beta with signal sequences from lipid-linked proteins, making them susceptible to enzymatic cleavage. In this manner, TCR heterodimers can be expressed independently of the CD3 polypeptides and in significant quantities (0.5 milligram per week). This technique seems generalizable to biochemical and structural studies of many other cell surface molecules as well.
Lin
A Y
AY
Howard Hughes Medical Institute, Stanford University School of Medicine, CA 94305-5402.
Devaux
B
B
Green
A
A
Sagerström
C
C
Elliott
J F
JF
Davis
M M
MM
D000469:Alkaline Phosphatase / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics; D017252:CD3 Complex; D018958:CD55 Antigens; D002460:Cell Line; D003169:Complement Inactivator Proteins / Q000235:genetics; D005260:Female; D006801:Humans; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D021382:Protein Sorting Signals / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D014162:Transfection
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D018958:CD55 Antigens; D003169:Complement Inactivator Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D021382:Protein Sorting Signals; D011948:Receptors, Antigen, T-Cell; D000469:Alkaline Phosphatase