Deoxyribonuclease zymography adapted to the precast PhastGel electrophoresis media.
A set-up for casting fluorescent indicator agarose gels on ultrathin polyacrylamide microelectrophoresis gel media (Pharmacia PhastGel media) is described. The zymogram system allows a rapid and sensitive detection of deoxyribonuclease in various gel media, following isoelectric focusing, native and sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Straetkvern
K O
KO
Department of Biochemistry, University of Bergen, Norway.
Raae
A J
AJ
D003851:Deoxyribonucleases / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D007525:Isoelectric Focusing; D012706:Serratia marcescens / Q000201:enzymology; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D007364:Intercalating Agents; D003851:Deoxyribonucleases; D004996:Ethidium