Molecular basis for linkage of a continuous and discontinuous neutralization epitope on the structural polypeptide VP2 of poliovirus type 1.
We obtained neutralizing monoclonal antibodies against a continuous neutralization epitope on VP2 of poliovirus type 1 strain Mahoney by using a combined in vivo-in vitro immunization procedure. The antibody-binding site was mapped to amino acid residues within the peptide segment (residues 164 through 170) of VP2 by competition with synthetic peptide and sequencing of resistant mutants. Cross-neutralization of these mutants with another neutralizing monoclonal antibody revealed a linkage of the continuous epitope and a discontinuous neutralization epitope involving both loops of the double-loop structure of VP2 at the twofold axis on the surface of the virion.
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Hamburg, Federal Republic of Germany.
K J
KJ
Wiegers
K
K
Wetz
R
R
Dernick
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D002213:Capsid / Q000235:genetics* / Q000276:immunology; D036022:Capsid Proteins; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis / Q000235:genetics*; D014644:Genetic Variation; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009500:Neutralization Tests; D017955:Poliovirus / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D000939:Epitopes; C442540:VP1 protein, Poliovirus