The effect of large doses of vitamin C and magnesium on stress responses in common carp, Cyprinus carpio.
1. Plasma magnesium, cortisol, lactate and ascorbic acid were examined in common carp subjected to various dietary treatments and following handling stress. 2. Under conditions of satisfied dietary magnesium and ascorbate requirements, plasma cortisol concentration after stress increased less pronouncedly than in fish fed large doses of ascorbate and/or magnesium. 3. Plasma lactate increased significantly in all groups after stress, although the increase seemed to be more severe (detrimental) in fish on large doses of ascorbate, either as ascorbic acid (AA) or ascorbic monophosphate Mg salt (AP). 4. Large doses of dietary ascorbate, both AA and AP, resulted in a significant increase of total ascorbate concentration in kidney and hepatopancreas of carp in comparison to pre-experimental level. 5. Kidney total ascorbate concentration decreased by 10-23% in all groups but one in which fish fed diet supplemented with AA displayed a significant increase (30%) of tissue ascorbate. The opposite trend was found in hepatopancreas of AA group with 21.5% ascorbate depletion. 6. The present results suggest that plasma cortisol and kidney (steroidogenesis site) and hepatopancreas ascorbate concentration responses to stress may not be related. Our results also do not support the hypothesis of the primary role of the high concentration of ascorbate in the kidney inhibiting steroidogenesis.
Department of Entomology, Ohio State University, Columbus 43210.
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Dabrowska
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Dabrowski
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Meyer-Burgdorff
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Hanke
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Gunther
D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002347:Carps; D006234:Handling, Psychological; D006854:Hydrocortisone / Q000097:blood; D007773:Lactates / Q000097:blood; D008274:Magnesium / Q000008:administration & dosage / Q000494:pharmacology*; D013312:Stress, Physiological / Q000378:metabolism*
D007773:Lactates; D008274:Magnesium; D001205:Ascorbic Acid; D006854:Hydrocortisone