Detection of nuclear cytomegalovirus antigen in cell culture as compared to classical virus isolation.
Rapid detection of human cytomegalovirus early nuclear protein in human diploid cells by immunofluorescence (IF) using commercial (Du Pont) monoclonal antibody was compared with the classical virus isolation in these cells. From 30 specimens tested early nuclear protein was detected in 16 cases but the virus was isolated out of 4 samples only.
Regional Station of Hygiene, Virology Department, Faculty Hospital, Králové, Czecho-Slovakia.
J
J
Horácek
P
P
Brucek
B
B
Otová
D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology* / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D017874:Immediate-Early Proteins; D008168:Lung / Q000382:microbiology; D014763:Viral Matrix Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D014763:Viral Matrix Proteins; C044582:immediate-early proteins, cytomegalovirus