Plasma volumes, blood volumes, and plasma protein concentrations after moderate haemodilution with fluosol-DA or normal saline in the rat.
Plasma volumes, blood volumes, and plasma total protein, albumin, and bilirubin concentrations have been determined in rats for 72 h following 20 or 40 mL kg-1 haemodilution with Fluosol-DA or 0.9% NaCl. Haemodilution with 20 mL kg-1 of either haemodiluent had no influence on the measured values. Plasma and blood volumes did not change after Fluosol-DA haemodilution at 40 mL kg-1, but albumin and bilirubin concentrations were decreased for 72 h. Only bilirubin concentrations were decreased for 72 h following haemodilution with 40 mL kg-1 of 0.9% NaCl. It was concluded that changes in a drug's plasma protein binding, and not the plasma or blood volume, are responsible for the reported alterations in a drug's apparent volume of distribution after haemodilution.
Shrewsbury
R P
RP
Division of Pharmaceutics, School of Pharmacy, University of North Carolina, Chapel Hill 27599-7360.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D001663:Bilirubin / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D001810:Blood Volume / Q000187:drug effects*; D004338:Drug Combinations; D005466:Fluorocarbons / Q000494:pharmacology*; D006438:Hemodilution; D006895:Hydroxyethyl Starch Derivatives; D007208:Indocyanine Green; D008297:Male; D010953:Plasma Volume / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains
D000418:Albumins; D001798:Blood Proteins; D004338:Drug Combinations; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination; D007208:Indocyanine Green; D001663:Bilirubin