Characterization of chromosomes and localization of the rDNA locus in the aye-aye (Daubentonia madagascariensis).
The karyotype of a prosimian primate, the aye-aye (Daubentonia madagascariensis), is described. Results from a variety of staining methods (Q-, R-, G-, and C-banding, distamycin A/DAPI and methyl-green/DAPI) are reported. Sites of methylation were visualized using antibodies against 5-methylcytosine. Digestion of aye-aye fixed metaphase chromosomes with the restriction endonuclease HaeIII produced G-banding. No other restriction enzymes tested produced clear G- or C-banding patterns. Ag-staining of the nucleolar organizer regions (NORs) revealed the location of rDNA sites on the short arms of the smallest pairs of acrocentric chromosomes, 13p and 14p.
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Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, MI 48201.
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Miller
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D044503:5-Methylcytosine; D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics*; D007621:Karyotyping; D008297:Male; D008745:Methylation; D012834:Silver; D013194:Staining and Labeling; D011443:Strepsirhini / Q000235:genetics*
D004275:DNA, Ribosomal; D012834:Silver; D044503:5-Methylcytosine; D003596:Cytosine; D004262:DNA Restriction Enzymes