[Immunohistochemical study on the keratin expression in generating rat hair tissues using anti-hair keratin monoclonal antibodies].
To investigate keratin expressions of generating rat hair tissues, monoclonal antibodies, HKN-2, 4, 5 and 6, were immunohistochemically applied on the skin specimens from 19-day-old rat fetus, newborn, 3-day-old and 7-day-old rats. In anagen hair tissues of 7-day-old rats, HKN-2 reacted with developing cells of the medulla, cortex, hair cuticle and inner and outer root sheaths. In the epidermis, HKN-2 reacted with spinous through horny cells. HKN-4 displayed a broad reactivity with all of the skin epithelial cells including hair tissue. These reactions were first seen in hair peg stage. HKN-2 and 4 first showed strong reactions with the innermost cell layer of the outer root sheath in anagen hair tissues of newborn rats. Reactivities by HKN-5 and 6 were localized in hair tissues in rats; however, HKN-5 showed no reaction with the innermost cell layer of the outer root sheath. HKN-5 and 6 showed no reaction with hair tissue before anagen. It is concluded that hair germ is different from epidermal basal cells in keratin expressions, that hair-specific keratins seem to be produced after the morphological differentiation of each layer, and that the innermost cell layer may differ from other outer root sheath since hair generation.
Department of Dermatology, Niigata University School of Medicine.
M
M
Takahashi
D000818:Animals; D000911:Antibodies, Monoclonal; D006197:Hair / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D007633:Keratins / Q000276:immunology / Q000378:metabolism*; D051381:Rats
D000911:Antibodies, Monoclonal; D007633:Keratins