The rat renin gene: assignment to chromosome 13 and linkage to the regulation of blood pressure.
It has recently been suggested that in the rat, sequence variation in the renin gene or closely linked genes may have the capacity to affect blood pressure and contribute to the pathogenesis of hypertension. To map the chromosomal location of the rat renin gene and to investigate its relationship to the inheritance of increased blood pressure, we studied a panel of rat x mouse somatic cell hybrids and a large set of recombinant inbred (RI) strains derived from spontaneously hypertensive rats (SHR) and normotensive Brown-Norway (BN) rats. We have found that in the rat, the renin gene is located on chromosome 13 and that it belongs to a conserved synteny group located on chromosome 1 in man and mouse. We have also found the median blood pressure of the RI strains that inherited the renin allele of the SHR to be greater than that of the RI strains that inherited the renin allele of the normotensive BN rat. These findings, together with the results of previous studies, suggest that in the rat, sequence variation in the renin gene, or in genes linked to the renin locus on chromosome 13, may have the capacity to affect blood pressure.
Pravenec
M
M
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
Simonet
L
L
Kren
V
V
Kunes
J
J
Levan
G
G
Szpirer
J
J
Szpirer
C
C
Kurtz
T
T
D000818:Animals; D001483:Base Sequence; D001794:Blood Pressure / Q000235:genetics*; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005649:Fumarate Hydratase / Q000235:genetics; D008040:Genetic Linkage / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D012083:Renin / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D015345:Oligonucleotide Probes; D012083:Renin; D005649:Fumarate Hydratase