Genomic heterogeneities in bovine herpesvirus type 1 viral isolates: a major variant selected from a field isolate.
We have analyzed viral DNA patterns, obtained following digestion of six different infectious bovine rhinotracheitis isolates, using a panel of restriction enzymes. Several differences in profiles were observed, particularly for a Canadian viral strain which presumably contained a genome that was larger than the other five infectious bovine rhinotracheitis genomes. Also, an insertion/deletion of a HindIII restriction site was found in the DNA of a Danish strain; this additional HindIII site was localized precisely within the original HindIII E fragment of the infectious bovine rhinotracheitis virus. Interestingly, results could be used to distinguish each viral strain examined from the others by simple digestion with the appropriate enzyme.
Simard
C
C
Centre de Recherche en Virologie, Université du Québec, Ville de Laval, Canada.
LaBoissière
S
S
Séguin
C
C
Trudel
M
M
D004279:DNA, Viral / Q000378:metabolism; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D005814:Genes, Viral; D014644:Genetic Variation; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII