Production, cross reactivity, and epitope analysis of monoclonal antibodies against rat kidney gamma glutamyltransferase.
Eighteen IgG1 monoclonal antibodies (Mabs) have been produced against gamma-glutamyl transferase (GGT) from rat kidney. They were specific to the light subunit of the enzyme with affinity constants ranging from 0.3 to 7.5 10(8) M-1, while they did not react with GGT from other sources i.e. human and pig kidney, rat and guinea pig liver, suggesting species and organ specificity. Two of the Mabs (No 11 and 21) lost their immunoreactivities towards rat kidney GGT in the presence of N-acetyl-neuraminic acid, while immunoreactivities of the other Mabs were unchanged. Furthermore, Mabs No 11 and 21 did not react with desialylated rat kidney GGT. These findings suggest that N-acetyl-neuraminic acid is involved in the epitopes recognized by these two Mabs.
Centre du Médicament, URA CNRS 597, Faculté des Sciences Pharmaceutiques et Biologiques, Nancy, France.
J
J
Bayad
N
N
Sabolovic
D
D
Bagrel
A
A
Visvikis
M
M
Wellman
G
G
Siest
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000915:Antibody Affinity; D015153:Blotting, Western; D002460:Cell Line; D003429:Cross Reactions; D003956:Dialysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D007668:Kidney / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009005:Monosaccharides / Q000494:pharmacology; D009439:Neuraminidase; D051381:Rats; D013045:Species Specificity; D013552:Swine; D005723:gamma-Glutamyltransferase / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D009005:Monosaccharides; D005723:gamma-Glutamyltransferase; D009439:Neuraminidase