Isolation and partial sequencing of a pancreatic polypeptide-like neuropeptide from the liver fluke, Fasciola hepatica.
1. A pancreatic polypeptide (PP)-immunoreactive neuropeptide has been isolated and partially sequenced from the liver fluke, Fasciola hepatica. 2. Gel permeation chromatography of an acid ethanol extract of cattle flukes showed that the peptide is similar in size to mammalian (bovine) PP. 3. The Fasciola peptide was purified to homogeneity by means of reverse-phase HPLC, employing different column chemistries. 4. The purified peptide was sequenced using automated gas-phase Edman degradation and the first 24 amino acid residues determined.
Comparative Neuroendocrinology Research Group, School of Biology, Queen's University of Belfast, Northern Ireland.
R M
RM
Magee
C
C
Shaw
I
I
Fairweather
L
L
Thim
C F
CF
Johnston
D W
DW
Halton
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005209:Fasciola / Q000378:metabolism*; D008969:Molecular Sequence Data; D010191:Pancreatic Polypeptide / Q000737:chemistry / Q000302:isolation & purification*; D011863:Radioimmunoassay
D010191:Pancreatic Polypeptide