Epitope mapping of an HLA-B27 monoclonal antibody that also reacts with a 35-kD bacterial outer-membrane protein.
Ye-3 is an HLA-B27-specific murine monoclonal antibody recognizing the heat-modifiable protein of Enterobacteriaceae. Here we used recombinant hybrid molecules between the HLA-B7 and HLA-B27 antigens to delineate the epitope recognized by Ye-3. Results of these experiments indicated that the segment of HLA-B*2705 spanning residues 77-81 was critical to the reactive epitope. It is known to be a major serological and functional recognition site of HLA-B*2705 and our data give support for its involvement also in the serological cross-reactivity with bacterial antigens.
INSERM U 283, Hôpital Cochin, Paris, France.
A
A
Toubert
M
M
Hamachi
C
C
Raffoux
M S
MS
Park
D T
DT
Yu
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D015153:Blotting, Western; D004755:Enterobacteriaceae / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D015796:HLA-B27 Antigen / Q000276:immunology*; D015793:HLA-B7 Antigen / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014162:Transfection
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D015796:HLA-B27 Antigen; D015793:HLA-B7 Antigen; D011993:Recombinant Fusion Proteins