Reduction of N-myc expression by antisense RNA is amplified by interferon: possible involvement of the 2-5A system.
Expression of myc-box genes can be reduced by Interferon (c-myc in Daudi cells) or Retinoic acid (N-myc in neuroblastoma cells). Interferon did not reduce N-myc expression in neuroblastoma cells. However, after transfection of the human neuroblastoma cell line LS with a vector, providing the Cadmium inducible expression of an antisense N-myc transcript, drastic reduction of N-myc RNA was achieved in these cells by incubation with Cadmium and Interferon. Treatment with Cadmium alone resulted in a comparably small reduction of N-myc transcripts in these cells. Interferon alone did not appreciably affect N-myc expression. Reduction of N-myc was accompanied with reduced cell proliferation and morphological differentiation. It is assumed that most of the inhibitory effects observed are mediated by the Interferon inducible 2-5A system.
Department of Hematology and Oncology, Children's Hospital, University of Tuebingen, FRG.
K
K
Schilbach
P
P
Pollwein
M
M
Schwab
R
R
Handgretinger
J
J
Treuner
D
D
Niethammer
G
G
Bruchelt
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D004273:DNA, Neoplasm / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D009447:Neuroblastoma / Q000235:genetics*; D009857:Oncogenes; D010957:Plasmids; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense; D012334:RNA, Neoplasm / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D007370:Interferon Type I; D016372:RNA, Antisense; D012334:RNA, Neoplasm; D012313:RNA; D015088:2',5'-Oligoadenylate Synthetase