[Application of high speed countercurrent chromatography for the isolation of natural products--preparative isolation of taxane-diterpenoids and diterpene alkaloids].
High speed countercurrent chromatography (HSCCC) based on the fundamental principle of liquid-liquid partition is a newly developed technique for isolation. It utilizes a particular combination of coil orientation and planetary motion to produce a unique hydrodynamic phenomenon in the unilateral phase distribution of two immiscible solvents in a coiled column. In recent years HSCCC has gained increasing popularity in the isolation of bioactive natural products. This paper reports its application in the preparative separation of taxol (1), cephalomannine (2) and baccatin-III (3) from the alcoholic extract of Taxus yunnanensis. These 3 ingredients are first obtained from this plant.
Rao
C
C
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Liu
X
X
Zhang
P L
PL
Chen
W M
WM
Fang
Q C
QC
D000470:Alkaloids / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D017239:Paclitaxel; D043823:Taxoids
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D043823:Taxoids; C073950:baccatin III; C056348:cephalomannine; D017239:Paclitaxel