IgM anti-idiotypes that block anti-HLA antibodies: naturally occurring or immune antibodies?
Using dithiothreitol (DTT) technique, IgM anti-HLA anti-idiotypic antibodies were detected in a multiparous multitransfused woman. These antibodies were able to inhibit the binding of specific IgG anti-HLA antibodies on their corresponding antigen. The recognized determinants were cross-reactive determinants since they were partially found on anti-HLA antibodies from unrelated individuals. By studying the patient's sera over a period of 2 years, no IgM-IgG switch was observed but the presence of these antibodies was stable in time, despite the disappearance of the idiotypes (anti-HLA antibodies). However, when looking at the patient's earlier serum, it was shown that these IgM anti-idiotypic antibodies were absent from the first available serum. Thus, these anti-idiotypic antibodies seem to behave both like natural and immune antibodies. The incidence of such antibodies in pretransplant patients is discussed.
Centre de Transfusion Sanguine de Strasbourg, France.
A
A
Urlacher
M M
MM
Tongio
J L
JL
Pasquali
D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000276:immunology*; D001803:Blood Transfusion; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D004229:Dithiothreitol; D000939:Epitopes / Q000276:immunology; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology*; D007674:Kidney Diseases / Q000276:immunology; D006435:Renal Dialysis; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D006680:HLA Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D004229:Dithiothreitol