Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction.
We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K+ channels to identify 8 different specific amplification products using poly(A)+ RNA isolated from islets of Langerhans from obese hyperglycemic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINm5F. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage-gated K+ channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin-producing cells.
Department of Pathology, University Hospital, Uppsala, Sweden.
C
C
Betsholtz
A
A
Baumann
S
S
Kenna
F M
FM
Ashcroft
S J
SJ
Ashcroft
P O
PO
Berggren
A
A
Grupe
O
O
Pongs
P
P
Rorsman
J
J
Sandblom
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006943:Hyperglycemia / Q000235:genetics / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007340:Insulinoma; D007515:Islets of Langerhans / Q000378:metabolism / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008820:Mice, Obese; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010190:Pancreatic Neoplasms; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000502:physiology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger
D007328:Insulin; D008565:Membrane Proteins; D015345:Oligonucleotide Probes; D015221:Potassium Channels; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA