Serotonin, dopamine, noradrenaline and their metabolites: levels in the brain of the house cricket (Acheta domesticus L.) during a 24-hour period and after administration of quipazine--a 5-HT2 receptor agonist.
1. The levels of 5-HT, DA, NA and DA metabolites (NADA, DOPAC) measured by HPLC (with electrochemical detection) in the brain of the house cricket did not change over a 24-hr period. The level of 5-HIAA, a 5-HT metabolite, was below the limit of detection. 2. The 5-HT and DOPAC levels decreased and NADA increased after quipazine injection but DA and NA levels did not change after it. 3. [3H]Ketanserin was used to identify serotonin receptors bound to sites in the house cricket brain with a KD of 5 nM and a concentration of Bmax 180 fmol/mg protein.
Zoological Museum, Institute of Zoology, Jagiellonian University, Kraków, Poland.
E
E
Pyza
K
K
Gołembiowska
L
L
Antkiewicz-Michaluk
D000818:Animals; D001921:Brain / Q000378:metabolism; D002940:Circadian Rhythm / Q000502:physiology; D004298:Dopamine / Q000378:metabolism*; D006135:Gryllidae / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism*; D011814:Quipazine / Q000494:pharmacology*; D011869:Radioligand Assay; D011985:Receptors, Serotonin / Q000187:drug effects*; D012701:Serotonin / Q000378:metabolism*
D011985:Receptors, Serotonin; D012701:Serotonin; D011814:Quipazine; D004298:Dopamine; D009638:Norepinephrine