A family of glutamate receptor genes: evidence for the formation of heteromultimeric receptors with distinct channel properties.
We have isolated two cDNA clones (GluR-K2 and GluR-K3) that share considerable sequence identity with the previously described glutamate receptor subunit, GluR-K1. The three glutamate receptor subunits show significant sequence conservation with the glutamine binding component of the glutamine permease of E. coli. Each of these clones encodes a channel responsive to both kainate and AMPA. The coexpression of GluR-K2 with either GluR-K3 or GluR-K1 results in the formation of channels whose current-voltage relationships differ from those of the individual subunits alone and more closely approximate the properties of kainate receptors in neurons. These observations indicate that the kainate/quisqualate receptors are encoded by a family of genes and are likely to be composed of hetero-oligomers of at least two distinct subunits.
Nakanishi
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Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Shneider
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Axel
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R
D026923:Amino Acid Transport Systems, Basic; D000818:Animals; D004594:Electrophysiology; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins; D005971:Glutamates / Q000378:metabolism; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007608:Kainic Acid / Q000378:metabolism / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D051381:Rats; D011919:Rats, Inbred Strains; D017470:Receptors, Glutamate; D018092:Kainic Acid Receptors; D017981:Receptors, Neurotransmitter / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid
D026923:Amino Acid Transport Systems, Basic; D029968:Escherichia coli Proteins; D005971:Glutamates; D007473:Ion Channels; D007608:Kainic Acid; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D017470:Receptors, Glutamate; D018092:Kainic Acid Receptors; D017981:Receptors, Neurotransmitter; C503373:glnP protein, E coli; C051432:glutamine permease