Chloride and potassium channels in U937 human monocytes.
Ionic channels in a human monocyte cell line (U937) were studied with the inside-out patch-clamp technique. A Ca2(+)-activated K+ channel and three Cl- -selective channels were observed. The Ca2(+)-activated K+ channel had an inward-rectifying current-voltage relationship with slope conductance of 28 pS, and was not dependent on membrane potential. Among the three Cl- channels, an outward-rectifying 28-pS channel was most frequently observed. The permeability ratio (Cl-/Na+) was 4-5 and CH3SO4- was also permeant. The channel became less active with increasing polarizations in either direction, and was inactive beyond +/- 120 mV. The channel, observed as bursts, occasionally had rapid events within the bursts, suggesting the presence of another mode of kinetics. Diisothiocyanatostilbene-disulfonic acid (DIDS) blocked the channel reversibly in a dose-dependent manner. The second 328-pS Cl- channel had a linear current-voltage relationship and permeability ratio (Cl-/Na+) of 5-6. This channel became less active with increasing polarizations and inactive beyond +/- 50 mV. DIDS blocked the channel irreversibly. The channel had multiple subconductance states. The third 15-pS Cl- channel was least frequently observed and least voltage sensitive among the Cl- channels. Intracellular Ca2+ or pH affected none of the three Cl- channels. All three Cl- channels had a latent period before being observed, suggesting inhibitory factor(s) present in situ. Activation of the cells with interferon-gamma, interferon-alpha A or 12-O-tetradecanoylphorbol-13-acetate (TPA) caused no change in the properties of any of the channels.
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
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D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D002460:Cell Line; D018118:Chloride Channels; D002712:Chlorides / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D007700:Kinetics; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D010539:Permeability; D015221:Potassium Channels / Q000378:metabolism*
D018118:Chloride Channels; D002712:Chlorides; D007473:Ion Channels; D008565:Membrane Proteins; D015221:Potassium Channels; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid