Rat gastric histamine release: a sensitive gastrin bioassay.
Acid secretion and histamine release from the totally isolated vascularly perfused rat stomach are sensitive parameters for the biological effects of gastrin. By optimalizing the Ca2+ concentration of the vascular perfusate the sensitivity for stimulation of acid secretion was improved from 65 to 16 pmol/l gastrin 1-17 (G-17). Measurement of venous histamine before and after gastrin in each stomach preparation excluded inter-stomach variability and improved the sensitivity to 2 pmol/l G-17. This is the most sensitive gastrin bioassay reported, with a detection limit comparable to that of radioimmunoassay.
Department of Medicine, University of Trondheim, Norway.
A K
AK
Sandvik
H L
HL
Waldum
D000818:Animals; D001681:Biological Assay; D004305:Dose-Response Relationship, Drug; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D005755:Gastrins / Q000032:analysis* / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D009924:Organ Culture Techniques; D011237:Predictive Value of Tests; D051381:Rats
D005755:Gastrins