Convulsants induce interleukin-1 beta messenger RNA in rat brain.
The effects of systemic administration of kainic acid and pentylenetetrazol on interleukin-1 beta gene expression in the rat brain was studied. After the administration of kainic acid in a convulsive dose (10 mg/kg i.p.), Interleukin-1 beta mRNA was induced intensely in the cerebral cortex, thalamus and hypothalamus, moderately in the hippocampus and weakly in the striatum, but not in the midbrain, pons-medulla and cerebellum. Pentylenetetrazol induced Interleukin-1 beta mRNA in the cerebral cortex, hypothalamus, and hippocampus with a faster time-course than kainic acid. Diazepam suppressed both the convulsion and the induction of Interleukin-1 beta mRNA produced by kainic acid. Dexamethasone suppressed the induction of Interleukin-1 beta mRNA, but did neither the convulsion nor the induction of c-fos mRNA following the injection of kainic acid. These results provide the first evidence that intensive neuronal excitation induces Interleukin-1 beta mRNA in particular regions of the brain.
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
M
M
Minami
Y
Y
Kuraishi
T
T
Yamaguchi
S
S
Nakai
Y
Y
Hirai
M
M
Satoh
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000276:immunology*; D003292:Convulsants / Q000494:pharmacology*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D003907:Dexamethasone / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D007375:Interleukin-1 / Q000235:genetics*; D007608:Kainic Acid / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D010433:Pentylenetetrazole / Q000494:pharmacology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains
D003292:Convulsants; D007375:Interleukin-1; D012333:RNA, Messenger; D012313:RNA; D003907:Dexamethasone; D004247:DNA; D003975:Diazepam; D007608:Kainic Acid; D010433:Pentylenetetrazole