Translational discrimination of mRNAs coding for human insulin-like growth factor II.
Sucrose gradient and Northern analyses were used to study the translational status of endogenous multiple mRNAs encoding the prepropeptide for human insulin-like growth factor II. The results showed that a minor 4.8-kilobase mRNA was exclusively engaged in the synthesis of the prepropeptide on membrane-bound polysomes, whereas a major 6.0-kilobase mRNA was present in a cytoplasmic particle which was stable in EDTA. We conclude that the translational discrimination between the mRNAs is dictated by their different 5'-untranslated regions.
Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen NV, Denmark.
F C
FC
Nielsen
S
S
Gammeltoft
J
J
Christiansen
D002460:Cell Line; D002874:Chromosome Mapping; D005091:Exons; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D012208:Rhabdomyosarcoma; D012270:Ribosomes / Q000378:metabolism; D013002:Somatomedins / Q000235:genetics*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D013002:Somatomedins; D011061:Poly A; D012313:RNA; D007335:Insulin-Like Growth Factor II