Immunochemical relationship of D-amino acid oxidases in various tissues and animals.
1. By means of an enzyme immunoassay, the contents of D-amino acid oxidase (DAO) were determined in kidney, liver, cerebellum and lung of hog, but the oxidase was not detectable in heart or cerebrum. 2. The oxidases in kidney, liver and cerebellum of hog were indistinguishable as regards immunoreactivity toward anti-hog kidney DAO antibody, specific activity and molecular weight. 3. The oxidases in rat and dog kidneys immunochemically cross-reacted with anti-hog DAO antibody. 4. The overall structure of the hog oxidase was more similar to that of the dog enzyme than that of the rat, while the structure around the catalytic site of the hog oxidase was more similar to that of the rat oxidase than that of the dog enzyme. 5. On immunoblot analysis, two forms of the oxidase were detected in extracts of hog, rat and dog kidneys.
Department of Biochemistry, Osaka University Medical School, Japan.
M
M
Katagiri
H
H
Tojo
K
K
Horiike
T
T
Yamano
D000818:Animals; D000906:Antibodies; D001921:Brain / Q000201:enzymology; D003429:Cross Reactions; D003605:D-Amino-Acid Oxidase / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D004285:Dogs; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D051381:Rats; D011921:Rats, Inbred WKY; D013045:Species Specificity; D013552:Swine
D000906:Antibodies; D003605:D-Amino-Acid Oxidase