Determination of poly(ADP-ribose) chain length distribution on polyacrylamide gels by silver staining.
Silver staining and polyacrylamide gel electrophoresis were used to visualize chain length distribution of poly(ADP-ribose) enzymatically synthesized from NAD by rat liver nuclei. The method described has the advantage that synthesis does not require radioactive-labeled NAD, and microgram quantities (greater than 5 micrograms) of poly(ADP-ribose) can be resolved and visualized as discrete bands according to chain lengths which range from 8 to 60 residues. This method can be applied to estimate size distribution of poly(ADP-ribose) chains in cells or tissues.
Hacham
H
H
Department of Biology, Technion-Israel Institute of Technology, Haifa.
Ben-Ishai
R
R
D002845:Chromatography / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008099:Liver / Q000378:metabolism; D015394:Molecular Structure; D009702:Nucleoside Diphosphate Sugars / Q000302:isolation & purification*; D011064:Poly Adenosine Diphosphate Ribose / Q000302:isolation & purification*; D012834:Silver; D013194:Staining and Labeling
D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose; D012834:Silver