Identification, sequencing, and expression of Mycobacterium leprae superoxide dismutase, a major antigen.
The gene encoding a major 28-kilodalton antigen of Mycobacterium leprae has now been sequenced and identified as the enzyme superoxide dismutase (SOD) on the basis of the high degree of homology with known SOD sequences. The deduced amino acid sequence shows 67% homology with a human manganese-utilizing SOD and 55% homology with the Escherichia coli manganese-utilizing enzyme. The gene is not expressed from its own promoter in E. coli but is expressed from its own promoter in Mycobacterium smegmatis. The amino acid sequences of epitopes recognized by monoclonal antibodies against the 28-kilodalton antigen have been determined.
Laboratory for Leprosy and Mycobacterial Research, National Institute for Medical Research, Ridgeway, London, United Kingdom.
H S
HS
Thangaraj
F I
FI
Lamb
E O
EO
Davis
P J
PJ
Jenner
L H
LH
Jeyakumar
M J
MJ
Colston
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D000939:Epitopes; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae / Q000235:genetics* / Q000276:immunology; D015183:Restriction Mapping; D013482:Superoxide Dismutase / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D013482:Superoxide Dismutase