Monoclonal antibodies against the human lymphocyte differentiation antigen CD 76 bind to gangliosides.
Two monoclonal antibodies, HD 66 and CRIS-4, by which the new CD 76 B-cell-associated cluster was defined, bound to several gangliosides (sialic acid containing glycolipids) of different polarity. One of the gangliosides recognized by HD 66 could be identified as NeuAc alpha 2-6Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc-beta 1-1'Cer. This antigen was enzymatically synthesized. Sialidase treatment of the ganglioside antigens abolished binding of HD 66 and CRIS-4.
GBF-Institute for Biotechnological Research, Braunschweig, FRG.
B
B
Kniep
P F
PF
Mühlradt
B
B
Dörken
G
G
Moldenhauer
R
R
Vilella
R
R
Schwartz-Albiez
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015703:Antigens, CD / Q000276:immunology*; D000943:Antigens, Differentiation / Q000276:immunology*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D000939:Epitopes / Q000276:immunology; D005732:Gangliosides / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; C063411:CD76 antigen; D000939:Epitopes; D005732:Gangliosides; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid