In vivo and in vitro immunosuppression by boar seminal vesicle fluid fraction.
Boar seminal vesicle fluid inhibits blast transformation of porcine lymphocytes. Boar seminal vesicle fluid was precipitated in 8% ethanol and the dissolved precipitate separated into two peaks upon chromatography on a Sephacryl S-200 column. The inhibitory activity was found predominantly in the second peak. This peak was rechromatographed on the Sephacryl S-200 column, and the fractions with inhibitory activity were pooled and passed down a Sephadex G-75 column. This chromatography run separated the accompanying protease inhibitor from the immunosuppressive fraction. The molecular mass, estimated by chromatography on the Sephadex G-75 column, was 25,000-27,000 for the immunosuppressive factor and 7,500 for the protease inhibitor. Activity for the immunosuppressive fraction was also demonstrated in vivo.
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
L
L
Veselský
D
D
Cechová
M
M
Hosková
J
J
Stédra
V
V
Holáñ
R
R
Stanék
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D007108:Immune Tolerance / Q000276:immunology*; D008297:Male; D012661:Semen / Q000276:immunology*; D013552:Swine