Expression of swine transmissible gastroenteritis virus envelope antigens on the surface of infected cells: epitopes externally exposed.
The peplomer protein (S) and the transmembrane protein (M) of transmissible gastroenteritis virus (TGEV) of swine were identified by iodination and serologically on the surface of infected cells. Of a total of 4 monoclonal antibodies (mAb) directed against four antigenic sites of S protein (Correa et al., 1988), 3 specific for sites A, B and D attached to the plasma membrane of infected cells, as disclosed by indirect immunofluorescence and by complement-mediated cytolysis. Four of the mAbs assayed were specific for the viral protein M and two of them gave plasma membrane immunofluorescence and mediated cytolysis in the presence of complement. The viral nucleoprotein N could not be demonstrated on the surface of infected cells either by iodination or employing 3 mAbs against this protein. Finally, a time course infection experiment demonstrated that S and M proteins were expressed on the surface of infected cells at 4 h after infection, before infective virus was released from infected cells.
Instituto Nacional de Investigaciones Agrarias, Departamento de Sanidad Animal, Madrid, Spain.
M D
MD
Laviada
S P
SP
Videgain
L
L
Moreno
F
F
Alonso
L
L
Enjuanes
J M
JM
Escribano
D000818:Animals; D000911:Antibodies, Monoclonal; D000920:Antibody-Dependent Cell Cytotoxicity; D000954:Antigens, Surface / Q000096:biosynthesis / Q000276:immunology*; D000956:Antigens, Viral / Q000096:biosynthesis / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D005761:Gastroenteritis, Transmissible, of Swine / Q000276:immunology*; D008297:Male; D013552:Swine; D013737:Testis / Q000276:immunology / Q000648:ultrastructure; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000956:Antigens, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins