[A simple method for separating fresh and cryopreserved human sperm using dextran-visotrast density gradient centrifugation].
A procedure for the separation of fresh and cryopreserved human spermatozoa is presented, compared with the Ficoll method according to Harrison [7] and clinically tested. The principle includes a filtration through glass wool and a gradient centrifugation. The gradient medium consists of Ca2(+)-free buffer, Dextran "70" and Visotrast, a radio-opaque medium. The sperm concentration, the sperm-migration-index and the sperm morphology checked by FACS 440 corresponds to the results of the Ficoll-method described by Harrison [7]. Furthermore, our method is usable for semen samples after adding of a cryoprotective medium with egg yolk and glycerol. An improvement of the chance of conception after artificial insemination by husband (AIH) by our method could not be found up to now.
Klinik für Hautkrankheiten, Karl-Marx-Universität Leipzig.
H J
HJ
Glander
J
J
Schaller
M
M
Ladusch
D002499:Centrifugation, Density Gradient / Q000379:methods*; D003287:Contrast Media; D003911:Dextrans; D003973:Diatrizoate; D003974:Diatrizoate Meglumine; D004338:Drug Combinations; D005260:Female; D005615:Freezing; D006801:Humans; D007315:Insemination, Artificial / Q000379:methods*; D007317:Insemination, Artificial, Homologous / Q000379:methods*; D008297:Male; D009845:Oligospermia / Q000628:therapy*; D011247:Pregnancy; D012662:Semen Preservation / Q000379:methods*; D013076:Sperm Count; D013081:Sperm Motility
D003287:Contrast Media; D003911:Dextrans; D004338:Drug Combinations; D003973:Diatrizoate; D003974:Diatrizoate Meglumine; C027278:urovision
Eine einfache Methode zur Separierung von frischen und kryokonservierten Humanspermien mittels einer Dextran-Visotrast-Dichtegradientenzentrifugation.