Extracellular matrix derived from Trypanosoma cruzi infected endothelial cells directs phenotypic expression.
Infection of confluent human umbilical vein endothelial cells by the parasite Trypanosoma cruzi results in the appearance of an altered heparan sulfate proteoglycan (HSPG) isolated from the extracellular matrix of infected endothelial cells (ECMi). HSPG from ECMi differed from HSPG obtained from the extracellular matrix of uninfected endothelial cells (ECMu) by virtue of an 8-10-fold increase in sulfation and a different elution pattern using DEAE Sepharose chromatography. Analysis of the HSPG that binds to acidic fibroblast growth factor (aFGF) revealed that infection increased the proportion of HSPG that binds to aFGF by 35%. Heparitinase and alkaline borohydride treatment of aFGF-binding HSPG and chromatographic resolution on Sepharose CL4B column revealed an infection-associated 10-fold increase in sulfation of the GAG side chain with no significant change in the migration of the core protein. In addition, the aFGF binding HSPG isolated from ECMi demonstrated a markedly attenuated synergistic mitogenic activity with aFGF in a cell proliferation assay. All of the infection associated changes in HSPG could be demonstrated in HSPG obtained from uninfected endothelial cell cultures grown on ECMi. Hence, the ECMi is associated with signals capable of modulating the ECM associated metabolism of uninfected endothelial cells. This facility of ECMi was also shown to extend to patterns of Gs protein synthesis as revealed by Western blot analysis. The observation that the ECM produced by infected endothelial cells can direct the synthetic patterns of uninfected endothelial cells in a manner uniquely observed in infected endothelial cells suggests a plausible pathway by which infection of only a few cells can ultimately result in the coordinate responses of neighboring uninfected cells.
Morris
S A
SA
College of Physicians and Surgeons, New York, New York 10032.
Wittner
M
M
Weiss
L
L
Hatcher
V B
VB
Tanowitz
H B
HB
Bilezikian
J P
JP
Gordon
P B
PB
D014355:Chagas Disease / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000469:parasitology; D005109:Extracellular Matrix / Q000378:metabolism*; D016220:Fibroblast Growth Factor 1 / Q000502:physiology; D019204:GTP-Binding Proteins / Q000096:biosynthesis; D006497:Heparan Sulfate / Q000378:metabolism / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008934:Mitogens; D011509:Proteoglycans / Q000378:metabolism / Q000502:physiology
D016220:Fibroblast Growth Factor 1; D019204:GTP-Binding Proteins; D006497:Heparan Sulfate; D008934:Mitogens; D011509:Proteoglycans