Fourier transform-Raman spectroscopy for the qualitative and quantitative characterization of sulfasalazine-containing polymeric microspheres.
FT-Raman spectroscopy (FTRS) has been used to characterize microspheres produced from the pharmaceutical polymer Eudragit RS containing a range of concentrations of the drug sulfasalazine. While pure sulfasalazine produced an intense and complex Raman spectrum, the spectrum of drug-free Eudragit RS microspheres was considerably weaker in intensity and contained only a few prominent Raman scattering peaks. In spectra of the drug-polymer microspheres, peaks arising from the individual components could be identified. This enabled a quantitative analysis to be undertaken by calculating the ratio between the area of a sulfasalazine peak and the area of a Eudragit RS peak for each microsphere spectrum. A correlation was shown between the peak area ratio and the microsphere sulfasalazine content. FTRS was then applied to a series of microsphere samples which had been dissoluted into pH 7 buffer for 1, 3, 6, 9, 12, or 24 hr. For each spectrum, the drug-polymer peak area ratio was determined and this in turn enabled calculation of the residual drug content of the microsphere sample. FTRS-calculated data showed good agreement with microsphere drug content values determined spectrophotometrically.
Watts
P J
PJ
Department of Pharmaceutical Sciences, Nottingham University, UK.
Tudor
A
A
Church
S J
SJ
Hendra
P J
PJ
Turner
P
P
Melia
C D
CD
Davies
M C
MC
D000180:Acrylic Resins; D003692:Delayed-Action Preparations; D005583:Fourier Analysis; D008863:Microspheres; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D012460:Sulfasalazine / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry*
D000180:Acrylic Resins; D003692:Delayed-Action Preparations; C050528:Eudragit RS; D012460:Sulfasalazine