Characterization of cross-reactive polysaccharide antigen with serotype a and d strains from Streptococcus sobrinus 6715.
The polysaccharide antigen (designated SI) from Streptococcus sobrinus 6715 (serotype g) which cross-reacts with serotype a and d strains was purified by a specific anti-cross-reactive g-a antibody-Sepharose 4B affinity column. By a double immunodiffusion analysis, the SI antigen was found to lack the serotype-specific g site, but contained the cross-reactive sites g-a, g-d and g-(a-d) on a single molecule. Polysaccharide SI was composed of galactose, glucose and rhamnose in a molar ratio of 4.79:1.52:1. The results of the test on the inhibition of the precipitin reaction and methylation analysis suggested that the cross-reactive site g-a of the SI antigen appeared to have two regions, one containing galactose residues and the other, beta-linked glucose residues.
Department of Microbiology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
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Takada
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Shiota
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Ikeda
D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D002236:Carbohydrate Conformation; D002846:Chromatography, Affinity; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D005779:Immunodiffusion; D008745:Methylation; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D011233:Precipitin Tests; D012703:Serotyping; D013295:Streptococcus mutans / Q000145:classification / Q000276:immunology*
D000942:Antigens, Bacterial; D000939:Epitopes; D011135:Polysaccharides, Bacterial