[Physico-chemical characterization of human seminal plasma specific antigen p30].
Human seminal plasma specific antigen p30, which has been characterized by other authors under the name of prostate-specific antigen or gamma-seminoprotein, has been purified. This communication describes the physico-chemical characterization of p30 demonstrated by various SDS-polyacrylamide gel electrophoresis and isoelectrofocusing as well as amino acid analysis. The results showed that the molecular weight of p30 was 33 kd and its isoelectric point was around pH 6.9. Its amino acid composition was similar to that of prostate-specific antigen. It is concluded that p30 purified by our laboratory and prostate-specific antigen named by Wang are the identical one of isomers.
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D000596:Amino Acids / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008970:Molecular Weight; D017430:Prostate-Specific Antigen; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis*; D029589:Seminal Plasma Proteins
D000596:Amino Acids; D000951:Antigens, Neoplasm; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D017430:Prostate-Specific Antigen