Monoclonal antibodies to glutathione S-transferase pi-immunohistochemical analysis of human tissues and cancers.
Mouse monoclonal antibodies (MAb) have been generated against the anionic isozyme of human glutathione S-transferase (GST pi). MAb AGST I can inhibit 50-70% of GST pi enzymatic activity and reacts with a 3-dimensional epitope which includes a putative glutathione binding site on GST pi. A sandwich enzyme-immunoassay established using MAb AGST I and a polyclonal antibody displayed a sensitivity of 0.5 ng/ml. Immunohistochemical analysis of human tissues demonstrated marked increases in GST pi levels in cancers of the brain, cervix, endometrium, colon, rectum and testis and in fibro- and chondrosarcomas.
Biological Carcinogenesis Development Program, Program Resources, Inc./DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21701.
R R
RR
Kantor
S L
SL
Giardina
A
A
Bartolazzi
A J
AJ
Townsend
C E
CE
Myers
K H
KH
Cowan
D L
DL
Longo
P G
PG
Natali
D000911:Antibodies, Monoclonal / Q000276:immunology; D001665:Binding Sites; D001932:Brain Neoplasms / Q000201:enzymology; D002813:Chondrosarcoma / Q000201:enzymology; D003110:Colonic Neoplasms / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D005354:Fibrosarcoma / Q000201:enzymology; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000032:analysis* / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D009369:Neoplasms / Q000201:enzymology*; D012004:Rectal Neoplasms / Q000201:enzymology; D013736:Testicular Neoplasms / Q000201:enzymology; D002583:Uterine Cervical Neoplasms / Q000201:enzymology; D014594:Uterine Neoplasms / Q000201:enzymology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007527:Isoenzymes; D005982:Glutathione Transferase; D005978:Glutathione