Requirement of MHC class I antigen expression on human tumors for in vitro recognition by autologous blood lymphocytes.
Expression of class I antigens on ex-vivo tumor cells was found to be required for their recognition by autologous blood lymphocytes in vitro. Lymphocyte stimulation (auto-tumor stimulation ATS) in mixed lymphocyte tumor cell cultures (MLTC) and cytolysis in short term assays (autologous lymphocyte-mediated cytotoxicity ALC) were positive with cells of a proportion of sarcoma and carcinoma patients. In addition to the class I antigens, these tumors also carried the adhesion molecule ICAM-1. Monoclonal antibodies (MAb) to the MHC class I, but not to class II antigens inhibited lymphocyte proliferation in the MLTC and lysis of tumor cells in the ALC tests. In a proportion of originally negative or low expressor tumors, in vitro treatment with Interferon gamma and tumor necrosis factor alpha induced the expression of class I antigens and/or ICAM-1. In the MLTC the cytokine treated tumor cells stimulated the blood lymphocytes and generated cytotoxic effectors which reacted also with the untreated tumor cells.
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
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D015815:Cell Adhesion Molecules / Q000502:physiology; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D000949:Histocompatibility Antigens Class II / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D009369:Neoplasms / Q000188:drug therapy / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015815:Cell Adhesion Molecules; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma