[Clinical studies of the specificity of detecting viral DNA in non-A, non-B hepatitis in liver tissue and lymphocytes].
The clinical specifity of an intraparticular virus-DNA of 5001 Bp associated with non-A, non-B hepatitis (HNANB) was evaluated. Investigations were done in liver biopsies and lymphocytes in 173 patients having acute or chronic HNANB (n = 107) or liver diseases of other etiology (n = 66). The sensitivity of the test system (polymerase chain reaction, southern-transfer, DNA-hybridisation with synthetic oligonucleotides) was less than 100 virus particles per probe. In all patients with acute HNANB (n = 5) (parenteral mode of infection) the DNA was found in 100% in liver and lymphocytes, and in 22 of 27 patients with acute sporadic HNANB. HNANB associated substance (HNANB-AS) (1.3 g/ml CsCl) excreted with feces was found in 50%, and 29.6%, respectively. In chronic HNANB the DNA was found in 83.3% in the liver (n = 42) and in 56.7% in lymphocytes (n = 30). The HNANB-AS was found in 45.6% (n = 68). In liver diseases with other etiologies as HNANB-infection (e.g. HBV, HAV, cholestasis, HBsAg pos.-liver cirrhosis) (n = 33) the DNA was found neither in liver biopsies nor in lymphocytes. In liver diseases of uncertain etiology, but with NANB-infection under discussion (e.g. nonspecific reactive hepatitis, fatty liver, HBV neg liver cirrhosis) the DNA was found in the liver in 24% (n = 25) and in lymphocytes in 40% (n = 5). In patients with clinically resolved HNANB no DNA was found in liver and lymphocytes (n = 5). All stools were negative for HNANB-AS in the latter.(ABSTRACT TRUNCATED AT 250 WORDS)
Med. Klinik I, Kliniken der Stadt Saarbrücken.
H
H
Liehr
M
M
Huth
D000956:Antigens, Viral / Q000032:analysis; D001706:Biopsy; D004279:DNA, Viral / Q000032:analysis*; D003937:Diagnosis, Differential; D005243:Feces / Q000737:chemistry; D006526:Hepatitis C / Q000473:pathology*; D018936:Hepatitis C Antigens; D006521:Hepatitis, Chronic / Q000473:pathology; D006801:Humans; D008099:Liver / Q000473:pathology*; D008214:Lymphocytes / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods
D000956:Antigens, Viral; D004279:DNA, Viral; D018936:Hepatitis C Antigens
Klinische Untersuchungen zur Spezifität des Nachweises einer bei Non-A-Non-B-Hepatitis auftretenden Virus-DNA im Lebergewebe und in Lymphozyten.