Detergent extraction of the human alpha-beta interferon receptor: a soluble form capable of binding interferon.
After examining a variety of detergents we find that receptors for human alpha interferon can be solubilized, in active form, from plasma membranes of lymphoid cells using the detergent CHAPS. The complexes formed, in solution, with interferon are stable enough to be separated chromatographically.
Laboratory of Viral Oncology (UPR 274/CNRS), Villejuif, France.
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D002793:Cholic Acids / Q000494:pharmacology*; D003902:Detergents / Q000494:pharmacology*; D006801:Humans; D007372:Interferons / Q000378:metabolism*; D008214:Lymphocytes / Q000032:analysis; D008970:Molecular Weight; D011971:Receptors, Immunologic / Q000302:isolation & purification* / Q000378:metabolism; D017471:Receptors, Interferon; D013501:Surface-Active Agents / Q000494:pharmacology*
D002793:Cholic Acids; D003902:Detergents; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D013501:Surface-Active Agents; D007372:Interferons; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate