New minimum length requirement for a T cell epitope for experimental allergic neuritis.
The bovine P2 protein induces experimental allergic neuritis (EAN) in Lewis rats. Using synthetic peptides representing regions of the P2 protein sequence and P2-specific neuritogenic T cell lines, we have demonstrated that the minimum peptide length requirement for the T cell epitope for EAN is residues 61-70 of the P2 protein having the sequence EISFKLGQEF. Adding a threonine at the NH2 terminus of this peptide reproduces residues 60-70 of the P2 protein sequence and significantly enhances the neuritogenic capability of this peptide.
Olee
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Immune Response Corporation, San Diego, CA 92121.
Powell
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Brostoff
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D000818:Animals; D000939:Epitopes / Q000032:analysis*; D004676:Myelin Basic Protein / Q000276:immunology*; D018996:Myelin P2 Protein; D009444:Neuritis, Autoimmune, Experimental / Q000209:etiology*; D010446:Peptide Fragments / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D004676:Myelin Basic Protein; D018996:Myelin P2 Protein; D010446:Peptide Fragments