Relationship of one form of human histamine-releasing factor to connective tissue activating peptide-III.
We have previously reported purification of three forms of histamine-releasing factors (HRFs) from mixtures of streptokinase-streptodornase stimulated human mononuclear cells and platelets with apparent molecular masses of 10-12, 15-17, and 40-41 kD (1989. J. Clin. Invest. 83:1204-1210). We have also prepared mouse MAbs against the 10-12-kD HRF (1989. J. Allergy Clin. Immunol. 83:281). Affinity-purified 10-12-kD HRF appears as a broad band upon polyacrylamide gel electrophoresis in the presence of SDS. We determined the NH2-terminal amino acid sequence of the top and bottom halves of this broad band. Sequence analysis revealed striking homology between this HRF and connective tissue activating peptide-III (CTAP-III), a platelet-derived 8-10-kD protein known to cause mitogenesis and extracellular matrix formation in fibroblast cultures. 19 of 21 NH2-terminal residues in the top half of the HRF band were identical to the NH2-terminal sequence of CTAP-III. 20 of 21 NH2-terminal residues in the bottom half were identical to the NH2-terminal sequence of neutrophil-activating peptide-2, which is derived from CTAP-III by proteolytic cleavage between residues 15 and 16. Purified CTAP-III also released histamine from basophils. Rabbit antiserum raised against either native or recombinant CTAP-III recognized affinity-purified HRF in immunodot blot assays, and MAb against HRF recognized CTAP-III in both dot blot and microtiter plate based immunoassays. These data demonstrate the first structural, functional, and immunologic relationship between one form of human HRF and a previously described cell product.
Department of Medicine, State University of New York, Stony Brook 11794-8161.
M L
ML
Baeza
S R
SR
Reddigari
D
D
Kornfeld
N
N
Ramani
E M
EM
Smith
P A
PA
Hossler
T
T
Fischer
C W
CW
Castor
P G
PG
Gorevic
A P
AP
Kaplan
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001491:Basophils / Q000187:drug effects / Q000502:physiology; D014408:Biomarkers, Tumor; D001792:Blood Platelets / Q000032:analysis; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000032:analysis*; D008222:Lymphokines / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009000:Monocytes / Q000032:analysis*; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D000090324:Tumor Protein, Translationally-Controlled 1
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D008222:Lymphokines; D010455:Peptides; D000090324:Tumor Protein, Translationally-Controlled 1; C009862:connective tissue-activating peptide