Modulation of DNA methylation and gene expression in cultured sycamore cells treated by hypomethylating base analog.
The selective suppression of photosynthetic genes in both the nuclear and plastid genomes of the nonphotosynthetic white wild-type cell line of sycamore (Acer pseudoplatanus) has been found to be inversely related to the presence of a variety of methylated bases, especially 5-methylcytosine (5-MeCyt) and N6-methyladenine (N6-MeAde), localized in regions of the plastid genome containing silent genes. We used hypomethylating base analogs to manipulate the level of cytosine and adenine methylation in the white cells of sycamore, and examined the effects of changes in methylation on gene expression. Treatment with 5-azacytidine (5-AzaCyd) and N6-benzyladenine (N6-BzlAde) decreased cytosine and adenine methylation. This was accompanied by restoration of transcriptional activity in photosynthetic genes which are usually suppressed. Both 5-MeCyt and N6-MeAde suppressed nuclear gene expression, but only 5-MeCyt suppressed plastid gene expression.
Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Japan.
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J
Ngernprasirtsiri
T
T
Akazawa
D015152:Blotting, Northern; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D006367:HeLa Cells; D008745:Methylation; D012313:RNA / Q000032:analysis; D014158:Transcription, Genetic; D014197:Trees
D012313:RNA; D004247:DNA