Immunohistochemical characterization of rat central and peripheral nerve tumors induced by ethylnitrosourea.
Ethylnitrosourea-induced central and peripheral nerve tumors in Sprague-Dawley rats were tested for GFAP (Glial Fibrillary Acidic Protein), S-100 protein, NSE (Neuron Specific Enolase) and Anti-Leu 7 (HNK-1) immunoreactivity utilizing the ABC method (avidin-biotin-complex) for GFAP, S-100 protein and NSE, and the PAP method (peroxidase-antiperoxidase) for Anti-Leu 7. Peripheral nerve neurinomas were consistently positive for S-100 protein and consistently negative for GFAP and Anti-Leu 7. Neurinomas would occasionally exhibit positive staining for NSE (2 of 55 tumors). The staining intensity for S-100 protein varied from strongly positive in differentiated neurinomas to weakly positive in anaplastic tumors. Neoplastic and reactive astrocytes exhibited positive staining for both S-100 protein and GFAP. Variation in the GFAP staining intensity of glial tumors correlated with the degree of differentiation as anaplastic tumors did not stain with the same intensity as their more differentiated counterparts. Oligodendrogliomas exhibited occasional immunoreactivity to S-100 protein (3 of 36 tumors). NSE reactivity in oligodendrogliomas was rarely observed (1 tumor in 36) and immunoreactivity against GFAP or Anti-Leu 7 was consistently absent. Anti-Leu 7 and NSE proved to be of little value in the classification of ENU-induced neural tumors.
Department of Pathology, Michigan State University, East Lansing 48824.
N R
NR
Raju
M J
MJ
Yaeger
D L
DL
Okazaki
K
K
Lovell
A
A
Koestner
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001254:Astrocytoma / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D018959:CD57 Antigens; D002490:Central Nervous System / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004806:Ependymoma / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D005038:Ethylnitrosourea / Q000633:toxicity*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005910:Glioma / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D008579:Meningioma / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D009423:Nervous System Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D009442:Neurilemmoma / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D009837:Oligodendroglioma / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D010524:Peripheral Nervous System Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000378:metabolism
D000943:Antigens, Differentiation; D018959:CD57 Antigens; D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins; D010751:Phosphopyruvate Hydratase; D005038:Ethylnitrosourea