Nerve growth factor stimulates phosphorylation of phospholipase C-gamma in PC12 cells.
PC12 cells contain at least three immunologically distinct phospholipase C (PLC) isozymes, PLC-beta, PLC-gamma, and PLC-delta. Treatment of PC12 cells with nerve growth factor (NGF) leads to an increase in the phosphorylation of PLC-gamma, but not of PLC-beta or PLC-delta. This increase can be seen in as little as 1 minute. The increased phosphorylation occurs on both serine and tyrosine residues, with the major increase being in the former. This result suggests the possibility that the NGF-dependent increase in phosphoinositide hydrolysis in PC12 cells is due to selective phosphorylation of PLC-gamma by serine and tyrosine protein kinases associated with the NGF receptor.
Signal Transduction Section, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
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UH
Kim
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D
Fink
H S
HS
Kim
D J
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Park
M L
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Contreras
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G
Guroff
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Rhee
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism; D009414:Nerve Growth Factors / Q000502:physiology*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D019000:Phosphotyrosine; D051381:Rats; D010738:Type C Phospholipases / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D007527:Isoenzymes; D009414:Nerve Growth Factors; D010750:Phosphoproteins; D010768:Phosphoserine; D019000:Phosphotyrosine; D014443:Tyrosine; D004815:Epidermal Growth Factor; D010738:Type C Phospholipases