[The use of the indirect fluorescent antibody technic for determining viral antigens in the urine of patients with hemorrhagic fever with renal syndrome].
The authors suggest a new technique for the detection of viral antigen in the urine of HFRS patients, based on the method for the differential cytologic rapid diagnosis of acute respiratory viral diseases. HFRS virus antigen was detectable in the urine sediment epithelium in 7 of the 8 patients from the seventh to the thirty-second day of the follow-up. Fluorescent bright-emerald dots could be seen in the urine sediment cells, situated on the cell surface, in the cytoplasm round the nucleus and in the periphery. In one case the antigen was not detected. No HFRS virus-injured cells were detected in the urine sediment epitheliocytes of normal subjects or patients with other renal diseases. The efficacy of the method was 87.5 +/- 12.5%. This method permits monitoring the virus persistence and its isolation from the patient during the acute period of the disease and early convalescence period. The technique is reproducible and takes 8-12 hrs; it can be used at research and practical laboratories.
Vereta
L A
LA
Elisova
T D
TD
D000956:Antigens, Viral / Q000652:urine*; D005455:Fluorescent Antibody Technique; D006476:Orthohantavirus / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology*; D006801:Humans
D000956:Antigens, Viral
Primenenie nepriamogo metoda fliuorestsiruiushchikh antitel dlia opredeleniia antigena virusa v moche bol'nykh gemorragicheskoĭ likhoradkoĭ s pochechnym sindromom.