Red cell autoantibodies characterized by competitive inhibition of iodine 125 Rh alloantibody binding and by immunoprecipitation of membrane proteins.
The relationship between determinants recognized by warm-type immunoglobulin G red cell autoantibodies and the Rh antigens was characterized by autoantibody competitive inhibition of iodine 125 Rh alloantibody binding and autoantibody immunoprecipitation of iodine 125 red blood cell membrane proteins. The majority of blood donor autoantibody recognized epitopes that are closely related to Rh antigens as determined by competitive inhibition studies. Eighteen of 20 (90%) autoantibodies inhibited anti-Rh(c) binding, 15 inhibited anti-Rh(E), 5 inhibited anti-Rh(D), and only 2 failed to inhibit any of the three Rh alloantibodies tested. Autoantibodies that inhibited anti-Rh(D) also inhibited anti-Rh(c) and anti-Rh(E) and all those that inhibited anti-Rh(E) also inhibited anti-Rh(c). Autoantibodies that inhibited all three Rh alloantibodies immunoprecipitated 30 kd membrane polypeptides, as did two of the three autoantibodies that inhibited only anti-Rh(c) and anti-Rh(E). One autoantibody in this group and two autoantibodies that inhibited only anti-Rh(c), as well as an autoantibody that did not inhibit any of the Rh alloantibodies, immunoprecipitated only a single membrane polypeptide identified as band 3. The majority of normal donor red blood cell autoantibodies inhibited the binding of Rh alloantibodies, which indicates that they either bound to the Rh polypeptides or to epitopes on band 3 that were closely associated with the Rh complex.
Department of Pathology, University of California, San Diego, La Jolla 92093.
S W
SW
Pierce
E J
EJ
Victoria
S P
SP
Masouredis
D001457:Anion Exchange Protein 1, Erythrocyte / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology*; D001667:Binding, Competitive; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007457:Iodine Radioisotopes; D007518:Isoantibodies / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D011233:Precipitin Tests; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D001457:Anion Exchange Protein 1, Erythrocyte; D001323:Autoantibodies; D000939:Epitopes; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D007518:Isoantibodies; D008565:Membrane Proteins; C033242:RH-antibodies; D012204:Rh-Hr Blood-Group System