Outer membrane protein profiles of Edwardsiella ictaluri from fish.
Outer membrane proteins (OMP) prepared with sodium N-lauroyl sarcocinate (SLS) from 33 Edwardsiella ictaluri isolates from fish were examined by electrophoresis. Twenty-eight isolates from channel catfish (Ictalurus punctatus) had similar OMP profiles. Ten bands (71 kilodaltons [kD] to 19.5 kD) were identified in all isolates from channel catfish. One major 35-kD protein comprised most of the protein content of the outer membrane of isolates from channel catfish. Differences existed among isolates in the amount of protein within minor OMP bands. Edwardsiella ictaluri ATCC 33202 contained larger quantities of the 38.5- and 37-kD proteins than did the other isolates. Outer membrane protein profiles of E ictaluri derived from Bengal danio (Danio devario) and walking catfish (Clarias batrachus) were identical to OMP profiles of isolates from channel catfish. In contrast, OMP profiles from single isolates from green knife fish (Eigemannia virescens) and white catfish (Ictalurus catus) were different. Variations in incubation time, SLS extraction time, SLS extraction number, and in vivo and in vitro passage had no effect on the OMP profile of E ictaluri ATCC 33202. An increase in duration of sample solubilization did affect the OMP profile of E ictaluri ATCC 33202 by decreasing the amount of protein in 52-, 46-, and 43.5-kD bands. Accompanying the decrease were increased staining intensity in the 31.5- and 28.5-kD bands and the appearance of 4 new bands (34, 33, 25.5, and 22.5 kD). Edwardsiella ictaluri, a gram-negative bacterium in the family Enterobacteriaceae, is the cause of enteric septicemia of catfish.(ABSTRACT TRUNCATED AT 250 WORDS)
Newton
J C
JC
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-3501.
Blevins
W T
WT
Wilt
G R
GR
Wolfe
L G
LG
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000662:veterinary; D002397:Catfishes / Q000382:microbiology*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000032:analysis* / Q000254:growth & development; D005399:Fishes / Q000382:microbiology; D007059:Ictaluridae / Q000382:microbiology*; D008970:Molecular Weight; D012521:Sarcosine / Q000031:analogs & derivatives; D013997:Time Factors
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; C025231:sarkosyl; D012521:Sarcosine