[Histamine release from human leukocytes by human recombinant interleukin-3].
Human recombinant interleukin-3 (hrIL-3) released histamine from human leukocytes in vitro. The histamine release by hrIL-3 significantly correlated with those by anti-IgE, thrombin, and f-met. peptide, but not by A23187. Histamine release by hrIL-3 was a Ca2(+)-dependent reaction, as was that by anti-IgE, although the time course of histamine release by hrIL-3 was slower than that by anti-IgE. Pre-treatment of leukocytes with hrIL-3 decreased the histamine release by hrIL-3 itself, but enhanced the histamine releases by anti-IgE, f-met. peptide, thrombin and A23187. These results suggested that the mechanism of hrIL-3-induced histamine release was different from those of anti-IgE, f-met. peptide, thrombin, and A23187. There was no significant difference between hrIL-3-induced histamine release of leukocytes from asthmatics and healthy controls.
Department of Pediatrics, Fujita Health University, School of Medicine.
S
S
Kanamori
A
A
Urisu
K
K
Iimi
Y
Y
Kondo
F
F
Horiba
S
S
Masuda
M
M
Tsuruta
T
T
Yazaki
D001249:Asthma / Q000097:blood; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects*; D011994:Recombinant Proteins
D007377:Interleukin-3; D011994:Recombinant Proteins