Polymerase chain reaction and allele-specific oligonucleotides in paternity testing of the deceased.
The assignment of paternity when the alleged father has died is now possible by use of a variety of allele-specific oligonucleotides after amplification of genomic DNA by the polymerase chain reaction (PCR). Issues relating to the inheritance of estates may be decided on fact rather than allegation. PCR-based genotyping of DQ alpha haplotypes from paraffin-embedded tissue of the deceased was used to prove non-paternity in the case reported here. Because the child was female, it was also possible to confirm the exclusion by using a second polymorphic site located in the factor VIII gene on the X chromosome.
Department of Pathology, University of Vermont, Burlington.
P L
PL
Howard
C C
CC
Collins
N H
NH
Heintz
D000483:Alleles; D015252:Deoxyribonucleases, Type II Site-Specific; D005169:Factor VIII / Q000235:genetics*; D005260:Female; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D015345:Oligonucleotide Probes; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D014960:X Chromosome
D006683:HLA-DQ Antigens; D015345:Oligonucleotide Probes; D005169:Factor VIII; C041666:endodeoxyribonuclease BclI; D015252:Deoxyribonucleases, Type II Site-Specific