Escherichia coli glyA mRNA decay: the role of 3' secondary structure and the effects of the pnp and rnb mutations.
The Escherichia coli glyA structural gene is followed by two REP sequences and a rho-independent transcription terminator. These sequences are essential for maintaining glyA mRNA stability and gene expression by blocking the 3' to 5' exonucleolytic activities of polynucleotide phosphorylase and ribonuclease II. The results support the model of cooperative endonucleolytic and 3' to 5' exonucleolytic activities in mRNA decay.
Department of Microbiology, University of Iowa, Iowa City 52242.
M D
MD
Plamann
G V
GV
Stauffer
D001483:Base Sequence; D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D012696:Glycine Hydroxymethyltransferase / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D011117:Polyribonucleotide Nucleotidyltransferase / Q000235:genetics; D011691:Puromycin / Q000494:pharmacology; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012260:Ribonucleases / Q000235:genetics; D014166:Transferases / Q000235:genetics*
D012329:RNA, Bacterial; D012333:RNA, Messenger; D011691:Puromycin; D002701:Chloramphenicol; D014166:Transferases; D012696:Glycine Hydroxymethyltransferase; D011117:Polyribonucleotide Nucleotidyltransferase; D012260:Ribonucleases