Organ distribution of the Ro (SS-A) antigen in the guinea pig.
Using an anti-Ro (SS-A)-specific "sandwich" enzyme-linked immunosorbent assay (ELISA) and Western immunoblotting, we determined the Ro (SS-A) content (both quantitative and qualitative) in saline-perfused organs of the guinea pig. All tissue extracts contained substantial concentrations of Ro (SS-A) antigen and could be grouped into 3 categories based on quantitative reactivity in the sandwich ELISA. The 60-kd and 52-kd molecular forms of Ro (SS-A) present in tissue extracts were similar to those described in Wi-L2 extracts, and the 54-kd molecular form of Ro (SS-A) in guinea pig erythrocytes was similar to that found in human erythrocytes. The tissue distribution of the isoforms of Ro (SS-A) was shown by Western immunoblotting to vary in different tissues, and the reactivity to the 60-kd Ro (SS-A) was correlated with the activity seen in the ELISA. Both the 60-kd and 52-kd Ro (SS-A) bands in guinea pig liver extracts were very weak on Western immunoblots, in contrast to the high concentration of Ro (SS-A) antigen in the ELISA. Other data suggest the possible existence of a unique form of Ro (SS-A) in the liver. Guinea pig tissues have 4 Y RNA that are equivalent to the 4 human RNA--hY1, hY3, hY4, and hY5--present in HeLa cells, while guinea pig red blood cells have only one Y RNA, which is equivalent in size to human hY4.(ABSTRACT TRUNCATED AT 250 WORDS)
Itoh
Y
Y
Arthritis/Immunology Research Program, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Kriet
J D
JD
Reichlin
M
M
D000818:Animals; D001324:Autoantigens / Q000032:analysis* / Q000235:genetics; D015153:Blotting, Western; D001923:Brain Chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D011233:Precipitin Tests; D012313:RNA / Q000032:analysis; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D013154:Spleen / Q000737:chemistry; D013270:Stomach / Q000737:chemistry; D014018:Tissue Distribution; D000099207:SS-A Antigen
D001324:Autoantigens; D012313:RNA; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human