Primary culture of astrocytic glial cells from rainbow trout, Salmo gairdneri L., brain.
A method is described for the preparation and maintenance of rainbow trout brain astrocytes in primary culture. A dissociated cell suspension was prepared from brains from young fish by mechanical sieving through nylon guazes, and the cells cultured in polylysine-treated plastic tissue culture flasks at 22 degrees C. The resultant cultures were characterised by specific immunofluorescent staining using glial fibrillary acidic protein (GFAP) for astrocytes and Thy 1 for fibroblastic cells. The cultures were greater than 95% astrocytes with fibroblasts the only other cell type present. These highly enriched astrocyte cultures provide a unique system for various neurochemical, neurophysiological and biochemical studies of fish neural cells. Polyunsaturated fatty acid metabolism will be an area of particular interest.
Tocher
D R
DR
NERC Unit of Aquatic Biochemistry, School of Natural Sciences, University of Stirling, Scotland, U.K.
Wilson
R
R
D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D002455:Cell Division; D003584:Cytological Techniques; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D013194:Staining and Labeling; D013997:Time Factors; D014337:Trout / Q000033:anatomy & histology*
D005904:Glial Fibrillary Acidic Protein