The cloned C-terminal region of a Trypanosoma cruzi P ribosomal protein harbors two antigenic determinants.
A DNA strategy was designed to characterize the antigenic site(s) within a lambda gt11 cloned 35-amino-acid antigenic peptide, identified with antibodies from patients with chronic Chagas' heart disease (cChHD) and systemic lupus erythematosus (SLE) as the C-terminal portion of a Trypanosoma cruzi P ribosomal protein. The 198-bp cDNA insert was digested with AluI, resulting in two DNA segments that were recloned in lambda gt11. To identify specific antigenic determinants, the recombinant phage and the purified recombinant antigens were probed with sera from clinically characterized subjects. Chronic ChHD and SLE sera defined a linear epitope common to both diseases within the 15 C-terminal residues of the parasite P ribosomal protein. It is also shown that the cloned 35-amino-acid peptide contained an antigenic site unique to cChHD.
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Buenos Aires, Argentina.
M J
MJ
Levin
R
R
Rossi
G
G
Levitus
E
E
Mesri
S
S
Bonnefoy
N
N
Kerner
M
M
Hontebeyrie-Joskowicz
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002598:Chagas Cardiomyopathy / Q000276:immunology; D003001:Cloning, Molecular; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010750:Phosphoproteins / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000276:immunology
D000939:Epitopes; D010750:Phosphoproteins; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D004247:DNA; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific