Activation of peritoneal macrophages with human cholesteatoma debris and alpha-keratin.
The effect of human cholesteatoma debris on mouse peritoneal macrophages was studied in vivo. The number of macrophages and lymphocytes increased 5 days after injection of the debris into the peritoneal cavity. A similar increase in peritoneal cells was observed when an urea-extracted fraction of the cholesteatoma debris or alpha-keratin, a major component of the debris, was injected. Both cholesteatoma debris- and alpha-keratin-elicited macrophages exhibited a greater response of luminol-dependent chemiluminescence upon exposure to zymosan, suggesting that the elicited macrophages were activated. In contrast, other constituents of the debris, such as cholesterol or fatty acid--with the exception of lipopolysaccharide (LPS)--failed to elicit or activate peritoneal macrophages at the similar doses detected in the debris. The chemiluminescent response of macrophages obtained by injecting LPS was, however, much lower than that of alpha-keratin-induced macrophages. These results indicate that cholesteatoma debris is capable of eliciting and activating macrophages, and that alpha-keratin is responsible for the activation.
Department of Otolaryngology, National Medical Center, Tokyo, Japan.
Y
Y
Iino
M
M
Toriyama
S
S
Ohmi
S
S
Kanegasaki
D000818:Animals; D001862:Bone Resorption / Q000209:etiology; D002781:Cholesteatoma / Q000276:immunology*; D004427:Ear Diseases / Q000276:immunology*; D006801:Humans; D007633:Keratins / Q000276:immunology*; D008163:Luminescent Measurements; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D008297:Male; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology
D007633:Keratins