The effects of pyrogallol and hydroquinone on the response to NANC nerve stimulation in the rat anococcygeus and the bovine retractor penis muscles.
1. The effects of pyrogallol and hydroquinone on the bovine retractor penis (BRP) and rat anococcygeus muscles to non-adrenergic, non-cholinergic (NANC) nerve stimulation have been examined. Both drugs at a concentration of 10(-4) M significantly reduced the response in the rat anococcygeus muscle but had no effect in the BRP muscle. 2. The inhibition of the NANC response in the rat anococcygeus muscle by pyrogallol was completely reversed by superoxide dismutase suggesting it was due to the generation of superoxide anions. 3. Pyrogallol inhibited the response to nitric oxide (NO) in the rat anococcygeus muscle but not that to 3-isobutyl-1-methyl-xanthine (IBMX) which confirmed a selective action. 4. These results suggest that the NANC neurotransmitter in the rat anococcygeus muscle is susceptible to superoxide anions and may be NO or a substance that can liberate NO.
Department of Pharmacology, University of Glasgow, Scotland.
J S
JS
Gillespie
H
H
Sheng
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001341:Autonomic Nervous System / Q000502:physiology*; D002417:Cattle; D004558:Electric Stimulation; D006873:Hydroquinones / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009569:Nitric Oxide / Q000494:pharmacology; D010413:Penis / Q000187:drug effects; D011748:Pyrogallol / Q000494:pharmacology*; D051381:Rats; D013481:Superoxides / Q000378:metabolism*; D019323:omega-N-Methylarginine
D006873:Hydroquinones; D011748:Pyrogallol; D013481:Superoxides; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D001120:Arginine; D015056:1-Methyl-3-isobutylxanthine; C031927:hydroquinone