Anti-idiotypic antibody identifies the structural similarity between the phosphatidylcholine-specific monoclonal antibody and phosphatidylcholine-specific lipid transfer protein.
The polyclonal anti-idiotypic antibody (Anti-Id) has been raised against a monoclonal antibody (mAb) that specifically binds to phosphatidylcholine (PC). The anti-Id bound strongly to PC-specific mAbs, but not to the other mAbs that bind to phosphatidylserine, indicating that the anti-Id recognizes the cross-reactive idiotopes expressed on the PC-specific mAbs. The anti-Id also showed an extensive cross-reaction with the PC-specific lipid transfer protein isolated from bovine liver and inhibited the lipid transfer activity of the protein. These results strongly suggest that the anti-Id recognizes a common structure shared between PC-specific mAbs and the PC-specific lipid transfer protein.
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
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Nam
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Umeda
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Igarashi
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Inoue
D000727:Androgen-Binding Protein; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007700:Kinetics; D010713:Phosphatidylcholines / Q000276:immunology / Q000378:metabolism*; D049294:Phospholipid Transfer Proteins; D011817:Rabbits / Q000276:immunology
D000727:Androgen-Binding Protein; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D010713:Phosphatidylcholines; D049294:Phospholipid Transfer Proteins