Multifunctional nature of P fimbriae of uropathogenic Escherichia coli: mutations in fsoE and fsoF influence fimbrial binding to renal tubuli and immobilized fibronectin.
P fimbriae of the F7(1) serotype of Escherichia coli are composed of a major subunit, FsoA, and of three minor proteins named FsoG, FsoE, and FsoF. FsoG is the Gal alpha(1-4)Gal-specific lectin. We assessed mutated recombinant strains each deficient in one fimbrial component for adhesion to frozen sections of rat cortical kidney and to fibronectin immobilized on glass. Rat kidney lacks the Gal alpha(1-4)Gal-containing glycolipids. The fsoG mutant strain was as adhesive to sections of rat kidney and to fibronectin-coated glass as was the recombinant strain expressing the complete fso gene cluster. The fsoA mutant strain was highly adhesive to fibronectin and to kidney sections. In the rat kidney, the adhesion of these strains was predominantly localized to sites of basolateral membranes of tubuli. The fsoE and the fsoF mutant strains were slightly less adhesive to kidney structures and failed to adhere to fibronectin. The fsoE, fsoF double mutant strain adhered neither to fibronectin nor to kidney sections. None of the fso recombinant strains reacted with soluble fibronectin, suggesting that the interaction is dependent on the conformation of the fibronectin molecules. Recombinant strains expressing the F7(2), F8, F11, F13, and F14 serovariants of the P fimbria also showed adherence to immobilized fibronectin. The results show that in addition to binding to globoseries of glycolipids via the G protein, the P fimbriae of uropathogenic E. coli exhibit a tissue-binding property influenced by fsoE and fsoF gene products and with affinity for basolateral membranes and fibronectin.
Westerlund
B
B
Department of General Microbiology, University of Helsinki, Finland.
van Die
I
I
Kramer
C
C
Kuusela
P
P
Holthöfer
H
H
Tarkkanen
A M
AM
Virkola
R
R
Riegman
N
N
Bergmans
H
H
Hoekstra
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D004926:Escherichia coli / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D005353:Fibronectins / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D005629:Frozen Sections; D006801:Humans; D007672:Kidney Cortex / Q000382:microbiology*; D007684:Kidney Tubules / Q000382:microbiology*; D008969:Molecular Sequence Data; D009154:Mutation; D051381:Rats; D011994:Recombinant Proteins; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D005353:Fibronectins; D011994:Recombinant Proteins; C074172:fsoE protein, E coli; C074171:fsoF protein, E coli