Proliferative response of different exocrine pancreatic cell types to hormonal stimuli. II. Effects of long-term secretin administration.
The effect of graded doses of secretin on exocrine pancreatic growth and cell proliferation was studied in a long-term experiment. After 16.5 and 150 micrograms/kg/24 h of secretin, each administered as two subcutaneous injections daily for 14 days, the pancreatic wet weight decreased, whereas the protein and DNA content of the gland was uninfluenced. When administered as described above for 14 days, 16.5, 50, 150, and 450 micrograms/kg/24 h of secretin did not affect the proliferation rate of ductal, acinar, or centroacinar cells as measured by a labeling index after 7 days of continuous 3H-thymidine administration. In the control groups a higher labeling index was found for ductal cells (19.9%) than for acinar cells (11.0%). During the 7 days of 3H-thymidine administration 80-90% of ductal and acinar cells remained in the G0 phase. There was a significantly higher labeling index in interlobular than in intralobular duct cells.
Dept. of Surgery, University Hospital, Linköping, Sweden.
T
T
Gasslander
S
S
Smeds
L
L
Blomqvist
I
I
Ihse
D000681:Amylases / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D051379:Mice; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism; D012633:Secretin / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D014316:Tritium
D014316:Tritium; D012633:Secretin; D004247:DNA; D000681:Amylases