Gamma irradiation alters bluetongue virus protein antigen.
Bluetongue virus (BTV) antigen, prepared for a monoclonal antibody (MAb)-based competitive enzyme-linked immunosorbent assay (C-ELISA), was exposed to 1, 2, 3, 4, 5 and 6 Mrad of gamma irradiation. The major group-specific BTV protein (VP7) reactive with the Mab was altered at higher doses of radiation, as revealed by immunoblotting studies. As well, a reduction in immunoreactivity was noted when irradiated antigen was used in the ELISA.
Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario.
A
A
Afshar
P
P
Moreau
G C
GC
Dulac
F C
FC
Thomas
D000818:Animals; D000956:Antigens, Viral / Q000528:radiation effects*; D015153:Blotting, Western; D001820:Bluetongue virus / Q000276:immunology* / Q000528:radiation effects; D002460:Cell Line; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D004797:Enzyme-Linked Immunosorbent Assay; D005720:Gamma Rays
D000956:Antigens, Viral