Isolation of nine human plasma proteinase inhibitors by sequential affinity chromatography.
Purification of nine plasma proteinase inhibitors and one zymogen from a single batch of human plasma, using affinity chromatography has been accomplished. Those isolated were plasminogen (lysine-Sepharose), alpha-2-antiplasmin (plasminogen-Sepharose), high and low molecular weight kininogens (CM-papain-Sepharose), alpha-2-macroglobulin (Zn++ chelate-Sepharose), alpha-1-proteinase inhibitor, alpha-1-antichymotrypsin, Cl-inhibitor, inter-alpha-trypsin inhibitor (Blue-Sepharose) and antithrombin III (heparin-Sepharose). Alpha-2-macroglobulin and alpha-1-proteinase inhibitor required gel filtration as additional purification steps. Each protein was recovered in both high yield and purity.
Dubin
A
A
Institute of Molecular Biology, Jagiellonian University, Cracow, Poland.
Potempa
J
J
Travis
J
J
D000510:Alpha-Globulins / Q000302:isolation & purification; D000990:Antithrombin III / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003174:Complement C1 Inactivator Proteins / Q000302:isolation & purification; D006801:Humans; D007704:Kininogens / Q000302:isolation & purification; D008259:Macroglobulins / Q000302:isolation & purification; D010958:Plasminogen / Q000302:isolation & purification; D011480:Protease Inhibitors / Q000097:blood / Q000302:isolation & purification*; D014361:Trypsin Inhibitors / Q000302:isolation & purification; D000514:alpha 1-Antichymotrypsin / Q000302:isolation & purification; D000515:alpha 1-Antitrypsin / Q000302:isolation & purification; D000979:alpha-2-Antiplasmin / Q000302:isolation & purification
D000510:Alpha-Globulins; D003174:Complement C1 Inactivator Proteins; D007704:Kininogens; D008259:Macroglobulins; D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; D000979:alpha-2-Antiplasmin; C011877:inter-alpha-inhibitor; D000990:Antithrombin III; D010958:Plasminogen