Human immunodeficiency virus infection of human bone marrow stromal fibroblasts.
The human immunodeficiency virus (HIV) preferentially infects CD4 positive T cells and monocytes. Other human cell types have been reported to be infectable with HIV, including cells of mesenchymal origin. In this report, we show that both primary human bone marrow stromal fibroblasts and an immortalized human stromal fibroblast line are susceptible to HIV infection. These cells are capable of passing HIV to cells of lymphoid or myeloid lineage, and may thereby act as a reservoir of virus. This in vitro system may be a useful model for assessing the pathophysiology of hematopoietic dysfunction in AIDS patients.
Division of Hematology/Oncology, New England Deaconess Hospital, Harvard Medical School, Boston, MA 02215.
D T
DT
Scadden
M
M
Zeira
A
A
Woon
Z
Z
Wang
L
L
Schieve
K
K
Ikeuchi
B
B
Lim
J E
JE
Groopman
D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells; D015704:CD4 Antigens / Q000235:genetics; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D015870:Gene Expression; D015683:Gene Products, gag / Q000032:analysis; D015488:HIV Antigens / Q000032:analysis; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D015498:HIV-2 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis; D014758:Viral Core Proteins / Q000032:analysis
D000199:Actins; D015704:CD4 Antigens; D004279:DNA, Viral; D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D014758:Viral Core Proteins; D012313:RNA; D004247:DNA