Epidemiological typing of uropathogenic Pseudomonas aeruginosa strains from hospitalized patients.
One hundred and twenty-one clinical isolates of Pseudomonas aeruginosa from patients with hospital-acquired urinary tract infections (UTIs) were studied to determine their major epidemiological markers, including API 20NE profile, O-serotype, pyocin type, phage type, lysogenic state and antibiotic susceptibility. Serotypes O4, O12, O11, O6 and O5 were found with a high frequency, accounting respectively for 23.9%, 23.1%, 12.3%, 8.2% and 5.7% of isolates. Pyocin type 10 was most common (32.2%) followed by types 1 (10.7%), 33 (7.5%) and 105 (4.1%); subtype h was predominant being characteristic of 34.7% of isolates. Most of the strains (69.4%) were either not phage typable or sensitive to phages 68 and 119x. Resistance to gentamicin, tobramycin, amikacin, imipenem and ciprofloxacin was more frequent among strains belonging to serotype O12. The O-serotypes were combined with API 20NE profiles, pyocin and phage types, lysogenic states and antibiotic resistance in order to identify epidemiologically related clones. Within predominant serotypes--O4 and O12--most strains displayed similar but not identical type characteristics, whereas other serotypes were less homogeneous. Our results support the concept that a combination of current typing techniques allows the identification of epidemiologically related P. aeruginosa isolates.
Institute of Microbiology, Faculty of Medicine, University of Rome La Sapienza, Italy.
P
P
Visca
F
F
Chiarini
C
C
Vetriani
A
A
Mansi
L
L
Serino
N
N
Orsi
D015373:Bacterial Typing Techniques; D001434:Bacteriophage Typing; D003428:Cross Infection / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000302:isolation & purification; D011709:Pyocins / Q000145:classification / Q000302:isolation & purification; D012703:Serotyping; D014552:Urinary Tract Infections / Q000382:microbiology
D011709:Pyocins