Colocalization of substance P and GABA in retinal ganglion cells: a computer-assisted visualization.
Ganglion cells in the albino rat retina were retrogradely labeled with the fluorescent dye, diamindino-yellow, from the superior colliculus. Preembedding and postembedding immunocytochemical techniques were employed in conjunction with computer-assisted image processing to visualize SP- and GABA-immunoreactivity. Examination of flatmount and sectioned retinas revealed that approximately 3% of the ganglion cells projecting to the contralateral superior colliculus exhibit SP-immunoreactivity. Moreover, these cells were found to comprise a subpopulation of the GABA-immunoreactive cells projecting to the rat tectum.
Department of Anatomy and Cell Biology, Wayne State University, Detroit, MI 48201.
D M
DM
Caruso
M T
MT
Owczarzak
R G
RG
Pourcho
D000818:Animals; D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D051381:Rats; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D013373:Substance P; D005680:gamma-Aminobutyric Acid