[Various approaches to designing the functionally active determinants as illustrated by the influenza virus A(H3N2) hemagglutinin].
Hexapeptide Lys-Gly-Pro-Asp-Ser-Gly analogous to the immunodominant fragment 141-146 of the epitope A of the influenza virus A(H3N2) hemagglutinin is synthesized. Conjugated with thyroglobulin and hemocyanine, the hexapeptide induced formation of highly specific antibodies with heterolytic properties in CBA mice. Antihexapeptide antibodies interact not only with the homologous antigen but also with hemagglutinin and influenza virus. Choice of the hexapeptide sequence is discussed.
Shibnev
V A
VA
Sharetskiĭ
A N
AN
Valiev
R V
RV
Khalikov
Sh Kh
ShKh
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry*; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000276:immunology*; D053122:Influenza A Virus, H3N2 Subtype; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D013954:Thyroglobulin / Q000276:immunology
D000914:Antibodies, Viral; D000939:Epitopes; D006389:Hemagglutinins, Viral; D013954:Thyroglobulin
Nekotorye podkhody v konstruirovanii funktsional'no-aktivnykh determinant na primere gemaggliutinina virusa grippa A(H3N2).