Structural analysis of the Hox-3.1 transcription unit and the Hox-3.2--Hox-3.1 intergenic region.
The mouse Hox gene family is a set of mammalian homeobox genes that may represent developmental control genes. Complete information about the primary structure of these genes is a prerequisite for a systematic analysis of the mechanisms that determine their complex tempero-spatial expression patterns. In this report we describe the complete sequence of the Hox-3.1 locus and provide evidence for several closely spaced transcriptional start sites. Sequence analysis of the 5' region of the Hox-3.1 gene extending to its nearest upstream neighbor, Hox-3.2, allowed us to identify sequences known to be capable of interactions with transcription factors. Several of these sequence motifs are similar to cis-regulatory elements found in the regulatory regions of other known developmentally regulated genes.
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425.
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Awgulewitsch
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Bieberich
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Bogarad
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Shashikant
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Ruddle
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D015723:Gene Library; D005801:Genes, Homeobox; D005809:Genes, Regulator; D018398:Homeodomain Proteins; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D015183:Restriction Mapping; D013116:Spinal Cord; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C489816:Hoxc8 protein, mouse; C065404:Hoxc9 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors; D011061:Poly A; D012313:RNA; D004247:DNA