Palytoxin induces an increase in the cation conductance of red cells.
Palytoxin (PTX), isolated from the marine soft coral Palythoa tuberculosa, increases the cation conductance of human red cell membranes. In the presence of 10(-10) M PTX and 10(-5) M DIDS, the membrane potential approximates the equilibrium potential for Na+ or K+ rather than Cl-. Even in the absence of DIDS, the Na+ and K+ conductances were greater than the Cl- conductance. The selectivity of the PTX-induced cation conductance is K+ greater than Rb+ greater than Cs+ greater than Na+ greater than Li+ much greater than choline+ greater than TEA+ much greater than Mg2+. Measurements of K+ efflux revealed two apparent sites for activation by PTX, one with a Kal of 0.05 nM and a maximum flux, nu max1, of 1.4 mol/liter of cells per h and another with a Ka2 of 98 nM and a nu max2 of 24 mol/liter of cells per h. These effects of PTX are completely blocked by external ouabain (300 microM) and prevented by internal vanadate (100 microM). When the PTX channels are open, the Na,K pumps do not catalyze ATP hydrolysis. Upon thorough washout of cells exposed to about five molecules of PTX/pump, the Na,K pump of these cells operates normally. Blockage of the positively charged NH2 terminus of PTX with a p-bromobenzoyl group reduces the potency of the compound to induce Na and K fluxes by at least a factor of 100, and to compete with the binding of [3H]ouabain by at least a factor of 10. These data are consistent with the conclusion that PTX binds reversibly to the Na,K pumps in the red cell membrane and opens a (10-pS) channel equally permeable to Na and K at or near each pump site.
Laboratory for Membrane Transport, Harvard Medical School, Boston, Massachusetts 02115.
M T
MT
Tosteson
J A
JA
Halperin
Y
Y
Kishi
D C
DC
Tosteson
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000178:Acrylamides; D000255:Adenosine Triphosphate / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002586:Cesium / Q000493:pharmacokinetics; D002712:Chlorides / Q000493:pharmacokinetics; D003064:Cnidarian Venoms / Q000494:pharmacology*; D004553:Electric Conductivity / Q000187:drug effects / Q000502:physiology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis / Q000187:drug effects; D008094:Lithium / Q000493:pharmacokinetics; D008274:Magnesium / Q000493:pharmacokinetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D011188:Potassium / Q000493:pharmacokinetics*; D015221:Potassium Channels / Q000187:drug effects; D012413:Rubidium / Q000493:pharmacokinetics; D012964:Sodium / Q000493:pharmacokinetics*; D015222:Sodium Channels / Q000187:drug effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects; D013997:Time Factors; D014638:Vanadates / Q000494:pharmacology; D000095702:Polyether Toxins
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D000178:Acrylamides; D000255:Adenosine Triphosphate; D002586:Cesium; D002712:Chlorides; D003064:Cnidarian Venoms; D008094:Lithium; D008274:Magnesium; D011188:Potassium; D015221:Potassium Channels; D012413:Rubidium; D012964:Sodium; D015222:Sodium Channels; D000254:Sodium-Potassium-Exchanging ATPase; D014638:Vanadates; C010272:palytoxin; D000095702:Polyether Toxins