In vitro isolation of a neutralization escape mutant of equine infectious anemia virus (EIAV).
A neutralization escape mutant (A/1 E) of equine infectious anemia virus was isolated after 13 passages in cell culture in the presence of serum containing antibodies to type- and group-specific determinants of EIAV envelope glycoproteins. Loss of neutralization by the selecting serum correlated with loss of two epitopes in the major envelope glycoprotein gp90 of A/1 E which were present in a parallel variant isolated from a persistently infected pony.
Department of Veterinary Microbiology and Parasitology, Louisiana State University, School of Veterinary Medicine, Baton Rouge.
P M
PM
Rwambo
C J
CJ
Issel
K A
KA
Hussain
R C
RC
Montelaro
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D015151:Immunoblotting; D004860:Infectious Anemia Virus, Equine / Q000235:genetics / Q000302:isolation & purification*; D009154:Mutation; D009500:Neutralization Tests; D012692:Serial Passage; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D014759:Viral Envelope Proteins