Isolation of an endogenously processed immunodominant viral peptide from the class I H-2Kb molecule.
Using an approach for isolating and characterizing peptide fractions that are intracellularly associated with major histocompatibility complex class I molecules, the major peptide recognized by cytotoxic T cells specific for the vesicular stomatitis virus has been isolated from the H-2Kb molecule of infected cells. This endogenously processed octapeptide is allele-specific as it does not bind to H-2Db molecules, and contains the core sequence of the epitope of the nucleocapsid protein of the vesicular stomatitis virus identified by testing with exogenous synthetic peptides.
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.
G M
GM
Van Bleek
S G
SG
Nathenson
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D002213:Capsid / Q000737:chemistry / Q000276:immunology; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D000939:Epitopes / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D006183:H-2 Antigens / Q000032:analysis*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D014758:Viral Core Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*
D000956:Antigens, Viral; D000939:Epitopes; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D015395:Histocompatibility Antigens Class I; D014758:Viral Core Proteins