Estimation of adenosine triphosphate utilization of rat mast cells during and after anaphylactic histamine secretion.
Determination of the cellular content of adenosine triphosphate (ATP) and the rate of ATP-synthesis were used to estimate the cellular utilization of ATP in relation to anaphylactic histamine secretion. There was an increased rate of oxidative ATP-synthesis and a decreased cellular ATP content during the time period of histamine secretion and immediately after its completion. During secretion the additional ATP-utilization above the basal level of ATP-synthesis was 0.51 pmol/10(3) cells. 2.5 min after cell activation, the rate of additional ATP-utilization was 0.30 pmol/10(3) cells/min, and the persistent ATP-decrease observed after 30 and 120 min may be due to a decreased rate of oxidative ATP-synthesis.
Johansen
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Department of Pharmacology, Medical School, Odense University, Denmark.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000707:Anaphylaxis / Q000378:metabolism*; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D003847:Deoxyglucose / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D005089:Exocytosis; D006636:Histamine Release; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D009840:Oligomycins; D000968:Antimycin A; D000255:Adenosine Triphosphate; D003847:Deoxyglucose