The complex of flavivirus envelope polypeptide with meningococcal proteosomes elicits formation of virus-neutralizing antibodies.
Polypeptide E of tick-borne encephalitis virus was isolated in sucrose density gradient and mixed with equal weight portion of meningococcal proteosomes in the presence of N-dodecyl-N,N-dimethylglycine. Mutual complexing of viral and bacterial molecules occurred after removal of detergent by dialysis. Complexed particles appeared in the electron microscope as 40-50 microns thick short-rod structures covered on their surface with both, delicate poppy-like grains, or envelope subunit-like clustered molecules. Even when applied without adjuvant, the complex of tick-borne encephalitis virus polypeptide E with meningococcal proteosomes elicited in mice a marked antiviral as well as antibacterial humoral response.
Institute of Virology, Slovak Academy of Sciences, Bratislava.
I
I
Slávik
P
P
Kuzemenská
O
O
Kozuch
J
J
Matoska
J
J
Pokorný
M
M
Sekeyová
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D051379:Mice; D009345:Neisseria meningitidis / Q000276:immunology*; D009500:Neutralization Tests; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D001425:Bacterial Outer Membrane Proteins; D014759:Viral Envelope Proteins