Human colon carcinoma cells with increased invasive capacity obtained by selection for sialyl-dimeric LeX antigen.
Sialyl-dimeric LeX antigen (NeuAc alpha 2-3Gal beta 1-4[Fuc-alpha 1-3] GlcNAc beta 1-3Gal beta 1-4[Fuc alpha 1-3]GlcNAc beta 1-R; SLX) is an oncodevelopmental carbohydrate antigen expressed both on glycolipids and on mucin-like glycoproteins in human colorectal carcinomas. The levels are higher in primary tumors at advanced stages than in tumors at early stages, and metastatic lesions contain a greater quantity of this antigen than corresponding primary tumors. To study whether this antigen influences the metastatic behavior of tumor cells, we selected SLX variant cells from the human colon carcinoma cell line KM12C using the specific monoclonal antibody FH6. We obtained two stable cell lines: a high expresser (KM12-HX) and a low expresser (KM12-LX) of this antigen. The growth rate of these cells are similar. A mucin-like glycoprotein reactive with monoclonal antibody FH6 was detected after electrophoretic separation of KM12-HX cell lysates but not of KM12-LX lysates. The degree of invasion was compared in assays in vitro using matrigel-coated filter membranes. The number of KM12-HX cells that invaded the membranes was significantly higher than KM12-LX cells.
Department of Tumor Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Y
Y
Matsushita
S
S
Nakamori
E A
EA
Seftor
M J
MJ
Hendrix
T
T
Irimura
D000911:Antibodies, Monoclonal / Q000276:immunology; D002455:Cell Division; D002469:Cell Separation / Q000379:methods; D015179:Colorectal Neoplasms / Q000276:immunology / Q000473:pathology*; D014644:Genetic Variation / Q000276:immunology; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology; D006801:Humans; D016256:Lewis X Antigen / Q000032:analysis / Q000276:immunology*; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid; D009361:Neoplasm Invasiveness / Q000276:immunology* / Q000473:pathology; D009362:Neoplasm Metastasis / Q000276:immunology / Q000473:pathology; D012794:Sialic Acids; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D016256:Lewis X Antigen; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid