An ultrastructural study of the guinea pig tectorial membrane 'type A' protofibril.
Fine structural features of the 'type A' protofibrils in the guinea pig tectorial membrane were examined using negative staining and deep-etching techniques. Negative-stained samples of fragmented tectorial membrane were composed of several fine filamentous subunits showing the clear banding pattern of the type A protofibrils. Deep-etched replicas of the EGTA (ethylene glycol bis-N,N,N',N'-tetraacetic acid)-treated samples showed fine surface structure consisting of several linear arrays of filamentous elements on the extracellular fibrils, which is interpreted to be type A protofibrils.
Department of Otolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
T
T
Arima
D J
DJ
Lim
H
H
Kawaguchi
Y
Y
Shibata
T
T
Uemura
D000818:Animals; D003051:Cochlea / Q000648:ultrastructure*; D003094:Collagen / Q000648:ultrastructure; D005613:Freeze Etching; D006168:Guinea Pigs; D008854:Microscopy, Electron; D013194:Staining and Labeling; D013680:Tectorial Membrane / Q000648:ultrastructure*
D003094:Collagen