[Hybridization analysis of nucleic acids using stained latex].
Method of the latex hybridization analysis has been developed: after hybridization of NA-target with biotinylated probe visualization of hybrids was carried out using latex particles, containing fluorescent dye pyronin G and coated with streptavidin. Due to encapsulation of the fluorescent dye in polymer particles sensitivity of the analysis was increased by several orders of magnitude in comparison with methods, using fluorescently labelled probes. Possessing a number of advantages, the method yields to none of any other methods of NA hybridization analysis in sensitivity.
Vener
T I
TI
Turchinskiĭ
M F
MF
Knorre
V D
VD
Generalova
A N
AN
Lukin
Iu V
IuV
Shcherbo
S N
SN
Turkin
S I
SI
Zubov
V P
VP
D007840:Latex; D008722:Methods; D009693:Nucleic Acid Hybridization; D013194:Staining and Labeling
D007840:Latex
Gibridizatsionnyĭ analiz nukleinovykh kislot s ispol'zovaniem okrashennykh lateksov.