A simple immunochemical technique for distinguishing melanocytes and melanophages in paraffin-embedded tissue.
We describe an immunoperoxidase technique for distinguishing melanocytes and melanophages in paraffin-embedded tissue. Two primary antibodies were used: anti-S100 and HAM-56, a monoclonal antibody directed against macrophages. In a variety of pigmented lesions, HAM-56 labelled melanophages while S100 labelled cells of melanocytic lineage.
Fleming
M G
MG
Cleveland Clinic Foundation, Ohio.
Bergfeld
W F
WF
D000686:Amyloidosis / Q000473:pathology; D000906:Antibodies; D000911:Antibodies, Monoclonal; D006644:Histiocytes / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008543:Melanins; D008544:Melanocytes / Q000473:pathology*; D008545:Melanoma / Q000473:pathology*; D009506:Nevus / Q000473:pathology*; D010232:Paraffin; D010587:Phagocytosis; D009418:S100 Proteins / Q000032:analysis; D012878:Skin Neoplasms / Q000473:pathology*; D013194:Staining and Labeling
D000906:Antibodies; D000911:Antibodies, Monoclonal; D008543:Melanins; D009418:S100 Proteins; D010232:Paraffin