A double-label study demonstrating that enkephalin and somatostatin are localized in separate populations of amacrine cells in the larval tiger salamander retina.
Previous studies have localized enkephalin and somatostatin to amacrine cell populations in the larval tiger salamander retina. Double-label immunocytochemistry was utilized to examine if enkephalin- and somatostatin-like immunoreactivities are colocalized to tiger salamander amacrine cells. Of the more than 2000 labelled cells observed in double-labelled preparations, none were found to express both enkephalin and somatostatin immunoreactivity. Therefore, these studies demonstrate that in the larval tiger salamander retina, enkephalin and somatostatin are localized to separate populations of amacrine cells.
Alice R. McPherson Laboratory of Retina Research, Baylor College of Medicine, Woodlands, TX 77381.
C B
CB
Watt
V J
VJ
Florack
D000557:Ambystoma; D000818:Animals; D004745:Enkephalins / Q000032:analysis / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D013004:Somatostatin / Q000032:analysis / Q000378:metabolism*
D004745:Enkephalins; D013004:Somatostatin