Effect of platelet-activating factor on cortisol and corticosterone secretion by perfused dog adrenal.
Administration of platelet-activating factor (PAF) to perfused adrenal increased cortisol and corticosterone secretion. With hexadecyl PAF (C16PAF; 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine), the increase was significant at 1 nM and maximal at 10 nM. The responses to 10 nM octadecyl PAF (C18PAF; 1-O-octadecyl-2-acetyl-sn-glycero-3-phosphocholine) were one fourth of those to 10 nM C16PAF. The addition of C16PAF to dispersed adrenal cells significantly increased cortisol and corticosterone production at 0.1 nM and 10 nM, respectively. C16PAF was about 1000 times more potent than histamine on a molar basis in respect to cortisol response in both perfused adrenal and dispersed adrenal cells. The results suggest that PAF induces cortisol release from dog adrenal.
Aikawa
T
T
Department of Physiology, Nagasaki University School of Medicine, Japan.
Hirose
T
T
Matsumoto
I
I
Morikawa
T
T
Shimada
T
T
Mine
Y
Y
Tsujimoto
Y
Y
Tsuji
Y
Y
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D003345:Corticosterone / Q000378:metabolism*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D006854:Hydrocortisone / Q000378:metabolism*; D007016:Hypophysectomy; D007700:Kinetics; D008297:Male; D009392:Nephrectomy; D010477:Perfusion; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000494:pharmacology; D012016:Reference Values
D000319:Adrenergic beta-Antagonists; D010972:Platelet Activating Factor; C037145:1-hexadecyl-2-acetyl-glycero-3-phosphocholine; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone; D006854:Hydrocortisone