Mapping genes encoding drug-metabolizing enzymes in recombinant inbred mice.
Probes for cytochrome P450IVA (P450IVA), alpha- and pi-class glutathione S-transferases (GST), and phenol-metabolizing UDP-glucuronyltransferase (UDPGT-K39) detected restriction fragment length variants (RFLVs) between C57BL/6J and DBA/2J mice. These variants were used to map the P450IVA genes (Cyp4 alpha) to chromosome 4, close to Mtv-13 and Pmv-19, midway between brown (b) and Gpd-1; GST alpha genes were mapped to chromosome 9, with a cross-hybridizing sequence mapping to another chromosome; the GST pi genes were mapped to the distal end of chromosome 1 near Pmv-21; one UDPGT-K39 variant to chromosome 1, between Acrg and Emv-17, and another showed linkage to Odc-10 on an unidentified chromosome. No RFLVs were detected with probes for P450IID, P450 reductase, androsterone-metabolizing UDPGT, GST mu, or microsomal GST.
Imperial Cancer Research Fund, University Department of Biochemistry, Edinburgh, United Kingdom.
J S
JS
Miles
J E
JE
Moss
B A
BA
Taylor
B
B
Burchell
C R
CR
Wolf
D000818:Animals; D002874:Chromosome Mapping; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D015342:DNA Probes; D014453:Glucuronosyltransferase / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D005810:Multigene Family; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats
D015342:DNA Probes; D003577:Cytochrome P-450 Enzyme System; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase