Zymographic screening of plant peroxidase isoenzymes oxidizing 4-hydroxystilbenes.
A zymographic assay is described for the detection of peroxidase isoenzymes oxidizing 4-hydroxystilbene following isoelectric focusing. The assay is based on coupling intermediate products of the oxidation of 4-hydroxystilbene with 4-aminoantipyrine, with resultant formation of dye complexes. Control experiments in the absence of 4-hydroxystilbene and hydrogen peroxide demonstrate the peroxidative nature of the 4-hydroxystilbene-dependent dye reaction.
Calderón
A A
AA
Department of Plant Biology, University of Murcia, Spain.
Pedreño
M A
MA
Ros-Barceló
A
A
Muñoz
R
R
D000675:Ampyrone / Q000378:metabolism; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000032:analysis* / Q000378:metabolism; D010944:Plants / Q000201:enzymology*; D013194:Staining and Labeling; D013267:Stilbenes / Q000378:metabolism*
D007527:Isoenzymes; D013267:Stilbenes; D000675:Ampyrone; C064297:4-hydroxystilbene; D010544:Peroxidases