Effects of preformed immune complexes on liver enzymes & their serum clearance in mice.
Two different performed HSA-anti-HSA immune aggregates, insoluble complex at equivalence (IC-E) and soluble complex with 5 times antigen excess (IC-S)-were administered iv in experimental mice to study their interaction with liver cells. Both complexes produced no appreciable change in the levels of liver enzymes like acid phosphatase, cathepsin D and gamma-glutamyl transferase. However, marked reduction in the level of liver pseduocholinesterase (as much as 93%) was recorded in the treated animals under identical conditions of administration of both the complexes. Hepatic uptake studies revealed that within 5 min, maximal sequestration of IC occurred within the liver (10 to 18%) and the blood (70 to 82%) when computed in terms of total injected radioactive IC. After 4 h, radioactivity dropped to 3 per cent in liver and 50-40 per cent in blood. The liver seemed to be incapable of scavenging all the serum complexes at a time. Significant consumption of serum complement occurred, when freshly prepared complexes were administered to the animals, but the reduced complement level showed a tendency to reach normalcy after 2 h. The soluble and equivalence zone IC failed to exhibit identifiable discrimination facets with respect to handling by liver. The complexes IC-E and IC-S also behaved in a similar manner.
Department of Biochemistry, University College of Medical Sciences, Delhi.
R K
RK
Kapoor
A K
AK
Tripathi
A K
AK
Chakrabarty
P
P
Sen
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism / Q000494:pharmacology*; D002091:Butyrylcholinesterase / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D006801:Humans; D007275:Injections, Intravenous; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D000936:Antigen-Antibody Complex; D005723:gamma-Glutamyltransferase; D002091:Butyrylcholinesterase; D000135:Acid Phosphatase; D002402:Cathepsin D