Localization of plant lipids for light microscopy using p-phenylenediamine in tissues of Arachis hypogaea L.
p-Phenylenediamine (pPD) can be used en bloc to preserve and differentiate cell lipids in aldehyde-fixed peanut plant tissues treated with osmium tetroxide during dehydration in 70% ethanol. Semithin plastic sections for light microscopy need no further staining and can be mounted in Histoclad after drying on a slide. Brown staining above background differentiates lipid-containing structures. Nonspecific staining can be distinguished in control preparations extracted en bloc with lipid solvents.
Department of Biology, Memorial University of Newfoundland, St. John's, Canada.
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D008055:Lipids / Q000032:analysis*; D010655:Phenylenediamines; D010944:Plants / Q000032:analysis*; D013194:Staining and Labeling
D008055:Lipids; D010655:Phenylenediamines; C029728:4-phenylenediamine