Growth hormone-releasing hormone and somatostatin influence neuronal expression in developing chick brain. III. GABAergic neurons.
We have shown that the endogenous neuropeptides, growth hormone-releasing hormone (GHRH) and somatostatin (SRIF) influence expression of both cholinergic and catecholaminergic neuronal phenotypes in developing chick brain as assessed by the activities of choline acetyltransferase and tyrosine hydroxylase, respectively (Dev. Brain Res., 49 (1989) 275-280; Brain Research, 512 (1990) 297-303). In this study we examined the effects of GHRH and SRIF on GABAergic neuronal expression in ovo using activity of glutamate decarboxylase (GAD) as a neuronal marker. Chick embryos were administered GHRH or SRIF in ovo via the air sac on embryonic days 1, 3, 5 and 7, sacrificed at day 8 and the activity of GAD assayed in whole brain homogenates. GAD activity was significantly reduced in peptide-treated embryos as compared to controls. Similar results were obtained when GHRH was administered in a single dose at days 1 or 3 or when SRIF was administered in a single dose at day 3; GAD activity was significantly reduced as compared with control embryos. In contrast, embryos treated with either GHRH or SRIF on day 5 of development showed no difference in GAD activity as compared to controls. These data support our previous findings that endogenous neuropeptides such as GHRH and SRIF possess important properties with respect to neuronal phenotypic expression. They further define the critical period of sensitivity to these neuropeptides as 1-3 days of embryonic development.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Pharmacology, University of Colorado School of Medicine, Denver 80262.
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Kentroti
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Vernadakis
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000196:embryology* / Q000502:physiology; D002642:Chick Embryo; D005314:Embryonic and Fetal Development; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D013004:Somatostatin / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D013004:Somatostatin; D005680:gamma-Aminobutyric Acid; D013007:Growth Hormone-Releasing Hormone; D005968:Glutamate Decarboxylase