Analysis of sulthiame in serum by narrow-bore high-performance liquid chromatography. Comparison of direct sample injection with pre-column switching and extrelut extraction.
Two high-performance liquid chromatographic methods for the analysis of sulthiame in serum are described. In the first method direct injection of serum samples onto a 4 x 4 mm I.D. (C18, 25 microns) precolumn in a column-switching device was used. After a purge step, the adsorbed analytes were eluted onto a 250 x 3 mm I.D. (C18, 5 microns) narrow-bore column for chromatographic separation. In the second method the sample pretreatment was an Extrelut extraction with dichloromethane-propanol-2 (95:5). After evaporation of the solvents, the residue was dissolved in methanol. The chromatographic separation was carried out on the same analytical column as used in the column-switching method. Both sample pretreatment methods were compared with respect to their suitability of routine analysis of sera from patients also receiving other antiepileptic drugs.
Juergens
U H
UH
Epilepsy Research Foundation, Department of Biochemistry, Bielefeld, Germany.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013843:Thiazines / Q000097:blood*
D013843:Thiazines