High-performance liquid chromatographic method for the evaluation of possible interferences in basophil-histamine release measurements.
Basophil activation studies commonly rely on the measurement of histamine following extraction and condensation to o-phthalaldehyde (OPT) as a quantitative measurement of degranulation. Specificity has long been recognized as a problem with this method. We have described a new high-performance liquid chromatographic method that allows for both a qualitative and a quantitative check of the purity of the OPT-histamine adduct. This method was sensitive (limit of detection = 2.55 pmol) and linear over a wide range (5 to 1000 ng/ml).
Siegel
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PD
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health (NIOSH), Morgantown, West Virginia 26505.
Lewis
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Olenchock
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SA
D015549:Basophil Degranulation Test; D001491:Basophils / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006636:Histamine Release; D006801:Humans; D009764:o-Phthalaldehyde / Q000032:analysis*
D009764:o-Phthalaldehyde