Isolation and regional localization of 35 unique anonymous DNA markers for human chromosome 22.
Thirty-five new, unique, DNA probes have been isolated and each has been assigned to one of five regions on chromosome 22. The distribution of probes along the chromosome is what would be expected based on the estimated size of each region with the exception of the short arm (22p). RFLP analysis was performed using 13 different restriction enzymes and over 50% of the probes were found to have useful polymorphisms. Probes mapping to 22q11 were further characterized by pulsed-field gel analysis and it has been possible to link several on large restriction fragments. These 35 new probes will be useful reagents for producing genetic and physical maps of chromosome 22.
Budarf
M L
ML
Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia 19104.
McDermid
H E
HE
Sellinger
B
B
Emanuel
B S
BS
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D015723:Gene Library; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA; D004262:DNA Restriction Enzymes