Detection of IgG, IgA, IgM and IgE antibodies in invasive amoebiasis in endemic areas.
Entamoeba histolytica-specific serum IgG, IgA, IgM and IgE antibodies were assayed in cases of amoebiasis in an endemic area. Patient groups consisted of amoebic liver abscess (n = 18), preabscess hepatic amoebiasis (n = 22) and amoebic colitis (n = 30). Control subjects comprised 26 asymptomatic cyst passers, 13 giardiasis cases, 20 typhoid patients and 24 non-amoebic individuals. Serum IgG was assayed by ELISA, using a monoclonal anti IgG beta-galactosidase (IgG beta-gal) conjugate, a polyclonal avidin biotin horse radish peroxidase (AB-HRP), and a polyclonal anti IgG horse radish peroxidase (IgG HRP) conjugate. IgA and IgM were assayed by the beta-gal ELISA and IgE by AB-HRP. Diagnostically significant IgG and IgA while lower IgM and IgE antibody levels were seen in extraintestinal cases. About 40% of suspected pre-abscess hepatic amoebiasis cases were confirmed by antibody estimation. All isotype levels in most dysentery cases were in the range of the controls.
Department of Microbiology, St. John's Medical College, Bangalore, India.
N
N
Shetty
S
S
Nagpal
P V
PV
Rao
H
H
Schröder
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D004404:Dysentery, Amebic / Q000097:blood / Q000453:epidemiology; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000097:blood / Q000453:epidemiology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005243:Feces / Q000469:parasitology; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis* / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis* / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology; D008101:Liver Abscess, Amebic / Q000097:blood / Q000453:epidemiology; D016036:Seroepidemiologic Studies
D000913:Antibodies, Protozoan; D000939:Epitopes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007073:Immunoglobulin E