A serum factor for macrophage activation after in vitro dodecylglycerol treatment of mouse lymphocytes.
Alkylglycerols, inflammation products of cancerous tissues, are potent macrophage activating agents. A brief in vitro treatment (30 min) of mouse peritoneal cells with a low concentration (50 ng/mL) of dodecylglycerol (DDG) in 10% foetal calf serum supplemented RPMI-1640 medium (FCS medium) activates macrophages for Fc-receptor mediated ingestion activity. A serum factor(s) was shown to be required for the activation of macrophages. When non-adherent cells were treated with rac-sn-1(3)-dodecylglycerol (DDG) in a serum free-0.1% egg albumin supplemented RPMI medium (EA medium) for 30 min and cultured in FCS medium for 2 h, the resultant conditioned medium contained a signal factor able to activate macrophages (macrophage activating factor). A conditioned medium prepared with electrophoresed serum alpha 2-globulin fraction in EA medium markedly enhanced activation of macrophages. Incubation of DDG-treated non-adherent cell ghosts in EA medium containing alpha 2-globulin also produced the macrophage activating signal factor. Therefore, it is concluded that a serum factor in alpha 2-globulin fraction is processed by pre-existing functions or enzymes of DDG-treated non-adherent cell membrane to yield a macrophage activating signal factor.
Department of Microbiology and Immunology, Hahnemann University School of Medicine, Philadelphia, PA 19102.
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S
Homma
I
I
Millman
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N
Yamamoto
D000510:Alpha-Globulins / Q000032:analysis / Q000276:immunology*; D000818:Animals; D002448:Cell Adhesion; D002462:Cell Membrane / Q000032:analysis; D005260:Female; D005989:Glycerides / Q000494:pharmacology*; D066298:In Vitro Techniques; D007848:Laurates / Q000494:pharmacology*; D007850:Lauric Acids / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D050178:Monoglycerides; D010529:Peritoneal Cavity / Q000166:cytology; D015398:Signal Transduction
D000510:Alpha-Globulins; D005989:Glycerides; D007848:Laurates; D007850:Lauric Acids; D050178:Monoglycerides; C020777:monolaurin