Notes on the application of microwaves in histopathology.
In this article two applications of microwaves in histopathology, microwave-stimulated staining of tissue sections and microwave-stimulated fixation of cryostat sections, are reviewed. For a good understanding of the influence of microwaves on physico-chemical processes like staining and fixation the relevant physics are included. Major advantages of microwave techniques are speed and/or improved quality. The cryostat-microwave technique appears to be well-suited for the demonstration of intermediate filament proteins: the sensitivity of monoclonal antibodies directed against keratins and vimentin can be substantially increased using the ethanol based fixative Kryofix.
Pathology Laboratory, Diakonessenhuis, Groningen, The Netherlands.
A J
AJ
Suurmeijer
M E
ME
Boon
L P
LP
Kok
D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008872:Microwaves; D013194:Staining and Labeling