DNA polymorphism at the casein loci in sheep.
By using seven endonucleases and four bovine cDNA probes specific for alpha S1-, alpha S2-, beta-, and kappa-casein genes, nine restriction fragment length polymorphisms (RFLPs) have been found in the sheep orthologous DNA regions. In contrast to the low level of variation observed at the protein level, these DNA polymorphisms determine a high level of heterozygosity and, therefore, represent useful tools for genetic analyses since they can also be obtained without the need for gene expression. In fact, informative matings suggest that in sheep, as in cattle, the four loci are linked.
Istituto di Produzione Animale, Facoltà di Agraria, Università della Basilicata, Italy.
P
P
Di Gregorio
A
A
Rando
E
E
Pieragostini
P
P
Masina
D000818:Animals; D001345:Autoradiography; D002364:Caseins / Q000235:genetics*; D003433:Crosses, Genetic; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D005260:Female; D008040:Genetic Linkage / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D006579:Heterozygote; D008297:Male; D010375:Pedigree; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012756:Sheep / Q000235:genetics*
D002364:Caseins; D015342:DNA Probes; D005819:Genetic Markers; D004720:Endonucleases