Marked genetic polymorphism of the swine steroid 21-hydroxylase gene, and its location between the SLA class I and class II regions.
Restriction fragment length polymorphism (RFLP) analysis of the swine 21-hydroxylase (CYP21) region was conducted on 31 unrelated SLA class I typed pigs, mainly Large Whites, including 15 haplotypes. Ten haplotypes were from SLA genotypic homozygotes and five were from SLA class I phenotypic homozygotes. DNA digestion with Hin dIII, TaqI and PstI, and hybridization to a 4.5-kb swine CYP21 genomic probe yielded respectively two, four and three RFLP patterns. Six patterns were identified with combined RFLP. In addition, analysis of the CYP21 region in families comprising several SLA recombinants demonstrated that the CYP21 gene lies in the DNA segment between the SLA class I and class II regions. These overall results reinforce our previous conclusion about the existence in the pig of a single 21-hydroxylase gene. The characterization of at least six CYP21 allelic patterns provides a new tool for studying the associations between the SLA region and zootechnical traits.
DSV-DPTE-LRA, Gif-sur-Yvette, France.
C
C
Geffrotin
C
C
Renard
P
P
Chardon
M
M
Vaiman
D000483:Alleles; D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005260:Female; D006239:Haplotypes; D008285:Major Histocompatibility Complex / Q000235:genetics; D008297:Male; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D013255:Steroid 21-Hydroxylase / Q000235:genetics*; D013552:Swine / Q000235:genetics* / Q000276:immunology
D015342:DNA Probes; D013255:Steroid 21-Hydroxylase; D004262:DNA Restriction Enzymes