[Fluorescent analogs of nucleoside-5'-phosphates for the study of nucleic acids by nonradioactive methods].
The synthesis of 2'-deoxyuridine 5'-triphosphate analogues with fluorescent residues of fluorescein and rhodamine nature at C5 of the uracil base was performed. Reverse transcriptase of avian myeloblastosis virus, DNA polymerase beta of rat liver, terminal deoxynucleotidyl transferase of calf thymus and E. coli DNA polymerase I, Klenow fragment, were shown to be capable to incorporate a nucleotide residue with fluorescent label into 3'-terminus of oligonucleotide. These fluorescent labeled oligonucleotides were used as primers for synthesis of (-)-chain of M13mp10 phage. Fluorescently labeling template-primer complexes were used for DNA sequencing.
L A
LA
Aleksandrova
M A
MA
Lukin
T A
TA
Rozovskaia
A M
AM
Atrazhev
M K
MK
Kukhanova
A A
AA
Kraevskiĭ
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004256:DNA Polymerase I / Q000378:metabolism; D003856:Deoxyuracil Nucleotides / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D051381:Rats
D003856:Deoxyuracil Nucleotides; D005456:Fluorescent Dyes; C027078:deoxyuridine triphosphate; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I
Fluorestsentnye analogi nukleozid-5'-trifosfatov dlia izucheniia nukleinovykh kislot neradioaktivnymi metodami.