The Epstein-Barr virus BNLF-1 membrane protein LMP1 induces homotypic adhesion mediated by CD11a/CD18 in a murine B-cell line, mimicking the action of phorbol ester.
Expression of the Epstein-Barr virus BNLF1 gene (LMP1) or treatment with 4B-phorbol-12,13-dibutyrate in the murine pro-B-cell line A/J-95 leads to a five- to sevenfold enhancement of homotypic adhesion without significant changes in adhesion molecule expression. Antibody to CD11a inhibits aggregation, indicating that CD11a/CD18-mediated adhesion is a common target for both agents.
Department of Immunology, Karolinska Institutet, Stockholm, Sweden.
R
R
Salcedo
S M
SM
Fuerstenberg
M
M
Patarroyo
G
G
Winberg
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000502:physiology*; D001402:B-Lymphocytes; D018845:CD11 Antigens; D018821:CD18 Antigens; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002460:Cell Line; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics*; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D009857:Oncogenes; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018845:CD11 Antigens; D018821:CD18 Antigens; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D009856:Oncogene Proteins, Viral; D014763:Viral Matrix Proteins; D015240:Phorbol 12,13-Dibutyrate; D011505:Protein-Tyrosine Kinases