Glutamate-induced inward current in a clonal neuroblastoma cell line.
The presence of glutamate-induced electrophysiological responses was examined in eight clonal neuroblastoma cell lines with the whole-cell voltage clamp technique. Only N2A cells responded to glutamate superfusion with a concentration-dependent, reversible inward current. Superfusion with the analogues kainic acid, quisqualic acid and N-methyl-D-aspartic acid also evoked inward currents but they had a smaller amplitude. The results indicate that N2A neuroblastoma cells could serve as an in vitro model to study the functional properties of glutamate receptors and associated ion channels.
Van der Valk
J B
JB
Research Institute of Toxicology, University of Utrecht, The Netherlands.
Vijverberg
H P
HP
D000818:Animals; D004594:Electrophysiology; D005971:Glutamates / Q000494:pharmacology*; D006801:Humans; D007473:Ion Channels / Q000187:drug effects*; D007608:Kainic Acid / Q000494:pharmacology; D051379:Mice; D016202:N-Methylaspartate / Q000494:pharmacology; D009447:Neuroblastoma / Q000378:metabolism*; D016318:Quisqualic Acid / Q000494:pharmacology; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
D005971:Glutamates; D007473:Ion Channels; D016202:N-Methylaspartate; D016318:Quisqualic Acid; D007608:Kainic Acid