Correction of the cystic fibrosis defect in vitro by retrovirus-mediated gene transfer.
We have used retrovirus-mediated gene transfer to demonstrate complementation of the cystic fibrosis (CF) defect in vitro. Amphotropic retroviruses were used to transduce a functional cystic fibrosis transmembrane conductance regulator (CFTR) cDNA into CFPAC-1, a pancreatic adenocarcinoma cell line derived from a patient with CF that stably expresses the chloride transport abnormalities characteristic of CF. CFPAC-1 cells were exposed to control virus (PLJ) and CFTR-expressing virus (PLJ-CFTR); viral-transduced clones were isolated and subjected to molecular and physiologic analysis. RNA analysis detected a viral-derived CFTR transcript in all of the PLJ-CFTR clones that contained unrearranged proviral sequences. Agents that increase intracellular cAMP stimulated 125I efflux in PLJ-CFTR clones but not PLJ clones. Whole-cell patch-clamp performed on three responding clones showed that the anion efflux responses were due to cAMP stimulation of Cl conductance. Our findings indicate that expression of the normal CFTR gene confers cAMP-dependent Cl channel regulation on CF epithelial cells.
Department of Internal Medicine, Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109-0650.
M L
ML
Drumm
H A
HA
Pope
W H
WH
Cliff
J M
JM
Rommens
S A
SA
Marvin
L C
LC
Tsui
F S
FS
Collins
R A
RA
Frizzell
J M
JM
Wilson
D000230:Adenocarcinoma / Q000235:genetics / Q000503:physiopathology; D002460:Cell Line; D018118:Chloride Channels; D002999:Clone Cells; D000242:Cyclic AMP / Q000378:metabolism; D003550:Cystic Fibrosis / Q000235:genetics*; D015316:Genetic Therapy; D006801:Humans; D007473:Ion Channels / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000503:physiopathology; D012190:Retroviridae / Q000235:genetics*; D014161:Transduction, Genetic; D014162:Transfection
D018118:Chloride Channels; D007473:Ion Channels; D008565:Membrane Proteins; D000242:Cyclic AMP