Genotoxicity of vanadium compounds in yeast and cultured mammalian cells.
The ability of vanadium compounds to induce genetic activity was investigated in D7 and D61M strains of Saccharomyces cerevisiae and in Chinese hamster V79 cell line. In our previous work, ammonium metavanadate (pentavalent form, V5) induced mitotic gene conversion and point reverse mutation in the D7 strain of yeast. The genotoxicity was reduced by the presence of S9 fraction, which probably reduced pentavalent vanadium to the tetravalent form. In the present study, vanadyl sulfate (tetravalent form, V4) induced no convertants and revertants in yeast cells harvested from stationary growth phase. With yeast cells from logarithmic growth phase, which contain high levels of cytochrome P-450, a significant increase in genetic effects was observed. Further experiments, performed by treating cells harvested from logarithmic growth phase in the presence of cytochrome P-450 inhibitors, indicated that the monooxygenase system influenced the genotoxicity of metavanadate while the genetic activity of vanadyl remained unaffected. Aneuploidy effect in the D61M strain of Saccharomyces cerevisiae was induced by either V5 or V4, confirming that vanadium compounds are potentially antitubulin agents in eukaryotic cells. Although these compounds are very toxic in V79 cells, no mutagenic effect was observed in the presence or in the absence of S9 fraction.
Galli
A
A
Istituto di Mutagenesi e Differenziamento CNR, Pisa, Italy.
Vellosi
R
R
Fiorio
R
R
Della Croce
C
C
Del Carratore
R
R
Morichetti
E
E
Giromini
L
L
Rosellini
D
D
Bronzetti
G
G
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D065607:Cytochrome P-450 Enzyme Inhibitors; D005785:Gene Conversion / Q000187:drug effects; D008099:Liver; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D014638:Vanadates / Q000633:toxicity*; D014639:Vanadium / Q000633:toxicity*; D017968:Vanadium Compounds
D065607:Cytochrome P-450 Enzyme Inhibitors; D009153:Mutagens; D017968:Vanadium Compounds; D014639:Vanadium; D014638:Vanadates; C034028:vanadyl sulfate