Molecular cloning of an invertebrate glutamate receptor subunit expressed in Drosophila muscle.
Insects and other invertebrates use glutamate as a neurotransmitter in the central nervous system and at the neuromuscular junction. A complementary DNA from Drosophila melanogaster, designated DGluR-II, has been isolated that encodes a distant homolog of the cloned mammalian ionotropic glutamate receptor family and is expressed in somatic muscle tissue of Drosophila embryos. Electrophysiological recordings made in Xenopus oocytes that express DGluR-II revealed depolarizing responses to L-glutamate and L-aspartate but low sensitivity to quisqualate, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA), and kainate. The DGluR-II protein may represent a distinct glutamate receptor subtype, which shares its structural design with other members of the ionotropic glutamate receptor family.
Zentrum für Molekulare Biologie, Universität Heidelberg, Federal Republic of Germany.
C M
CM
Schuster
A
A
Ultsch
P
P
Schloss
J A
JA
Cox
B
B
Schmitt
H
H
Betz
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D008969:Molecular Sequence Data; D009132:Muscles / Q000502:physiology*; D009841:Oligonucleotides / Q000737:chemistry; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment
D005971:Glutamates; D009841:Oligonucleotides; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D018698:Glutamic Acid; D004247:DNA