Enhancement of HIV-1 marker detection in cell cultures treated with mild heat-shock.
Rapid exposure to supra-optimal temperature (heat-shock) and a variety of other treatments are able to induce changes in cellular translational and transcriptional activity referred to as "the heat-shock response". The effect of heat-shock was investigated in H9 lymphoblastic cells and in peripheral blood lymphocytes infected with human immunodeficiency virus type 1 (HIV-1). The results showed that a mild heat-shock performed either immediately before infection or 7 days after infection consistently increased the recovery of p24 core antigen and reverse transcriptase activity in the supernatants of experimentally infected cell cultures. On the contrary, heat-shock had no effect on the HIV-1 marker recovery from persistently infected H9/HTLV-III cell cultures.
Microbiology Institute, University of Bologna, St. Orsola Hospital, Italy.
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Furlini
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Musiani
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Zerbini
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La Placa
D002452:Cell Count; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D015683:Gene Products, gag / Q000096:biosynthesis; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D014758:Viral Core Proteins / Q000096:biosynthesis; D014779:Virus Replication
D015683:Gene Products, gag; D016655:HIV Core Protein p24; D014758:Viral Core Proteins; D012194:RNA-Directed DNA Polymerase