Clinical patterns of ocular anaphylaxis in guinea pigs passively sensitized with IgG1 antibody.
A model of topically induced ocular anaphylaxis in guinea pigs was developed. A guinea pig anti-dinitrophenyl (DNP) bovine gamma-globulin antiserum rich in IgG1 antibodies was injected subconjunctivally; various serum dilutions and latent periods were tested. The anaphylactic response was elicited with di-DNP-lysine applied topically. The response was characterized by rating 11 ocular signs. Although the 4-hour latent period is considered optimal for passive cutaneous anaphylaxis, a 14-hour latent period yielded the strongest ocular reaction and appeared to the most appropriate time for clinical assessment. Both an early-phase reaction (peaking in the 21st hour) and a late-phase reaction (peaking at 6-10 h) were observed. The two signs of the early-phase reaction that recurred in the late-phase reaction were periorbital swelling and lid redness. The anaphylactic reaction displayed different patterns in different animals: protracted, biphasic and multiphasic.
Immunology Unit, Eye Research Institute, Boston, Mass.
A
A
Leonardi
R M
RM
Briggs
K J
KJ
Bloch
M R
MR
Allansmith
D000707:Anaphylaxis / Q000209:etiology / Q000276:immunology*; D000818:Animals; D003228:Conjunctiva / Q000378:metabolism; D003229:Conjunctival Diseases / Q000276:immunology*; D004195:Disease Models, Animal; D005141:Eyelid Diseases / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007116:Immunization, Passive; D007074:Immunoglobulin G; D009916:Orbital Diseases / Q000276:immunology*; D005719:gamma-Globulins
D007074:Immunoglobulin G; C030730:dinitrophenyl gamma globulin; D005719:gamma-Globulins