Suppression of human immunodeficiency virus replication by CD8+ cells from infected and uninfected chimpanzees.
Over a 4-year period, infectious human immunodeficiency virus type 1 (HIV-1) has been recovered from cultured peripheral blood mononuclear cells (PBMC) of virus-infected animals only intermittently and at relatively low titers. In examining the possible mechanism for this observation, CD4+ cells or CD8+ cells were removed by panning from the PMBC before culture. A dramatic increase in frequency of HIV-1 recovery as well as in the level of virus replication was observed in the CD4+ cell-enriched or CD8+ cell-depleted cultures of PBMC from 3/3 infected animals. Moreover, addition of purified CD8+ effector cells from all 6 HIV-infected and 5/10 uninfected animals to an equal number of HIV-1 acutely infected purified CD4+ target cells resulted in 75-100% suppression of virus production. CD8+ cells from 3 additional uninfected animals caused delayed replication kinetics and moderate to low suppression of peak virus production. These findings contrast with the previously recognized absence of this HIV-1-suppressing activity of CD8+ cells from seronegative humans. This CD8+ cell-mediated suppression of viral replication could help explain the natural resistance of chimpanzees to HIV-induced disease.
Castro
B A
BA
Cancer Research Institute, University of California, San Francisco 94143-0128.
Walker
C M
CM
Eichberg
J W
JW
Levy
J A
JA
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology; D016827:CD8 Antigens; D002470:Cell Survival; D006678:HIV / Q000254:growth & development* / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D007111:Immunity, Cellular; D002679:Pan troglodytes / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014779:Virus Replication
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens