Biochemical and morphological studies on perivenous and periportal liver parenchymal cells from female rats isolated by digitonin-collagenase method.
Periportal (pp) or perivenous (pv) liver parenchymal cells from female adult Uje: WIST rats were isolated after retro- or antegrade digitonin infusion followed by collagenase perfusion in the opposite direction. The morphological results revealed a distinct acinar-related destruction of the pv- or pp-zone by digitonin. The remaining cells of the respective other zone showed a good structural maintenance. After subsequent conventional collagenase perfusion the yield, viability and structural integrity of the isolated hepatocytes were high. The zonal cell separation was indicated by significant differences in the pp marker glucose-6-phosphatase and the pv marker glutamine synthetase found in the isolated pp or pv cell populations. Under our experimental conditions including the use of female rats, the alanine aminotransferase and glutamate dehydrogenase as well as ethylmorphine N-demethylase and ethoxycoumarin O-deethylase activities were evenly distributed in both preparations. Under stimulating conditions the capacity for urea synthesis was similar in both pv and pp cells.
Köhler
T
T
Institute of Clinical and Laboratory Diagnostics, Karl Marx University Leipzig, F.R.G.
Müller
D
D
Franke
H
H
Machnik
G
G
Müller
D
D
Zimmermann
T
T
D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D002469:Cell Separation / Q000379:methods; D004072:Digitonin; D005260:Female; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D006503:Hepatic Veins / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D003012:Microbial Collagenase; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D011168:Portal System / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D014508:Urea / Q000378:metabolism
D014508:Urea; D015660:7-Alkoxycoumarin O-Dealkylase; D005969:Glutamate Dehydrogenase; D010089:Oxidoreductases, N-Demethylating; D000410:Alanine Transaminase; D005952:Glucose-6-Phosphatase; D003012:Microbial Collagenase; D005974:Glutamate-Ammonia Ligase; D004072:Digitonin