Experimental mast cell activation improves connective tissue repair in the perforated rat mesentery.
The participation of mast cells in connective tissue repair was studied using the perforated-rat-mesentery model. Perforation of mesenteric membranes was performed during laparotomy of anesthetized Sprague-Dawley rats. Laparotomy significantly reduced the histamine content of the mesenteric membranes on day 1 postoperatively and perforation as such reduced the histamine content even more on days 1-10. Mast cell activation induced by a single intraperitoneal injection of Compound 48/80 two days prior to operation, significantly improved healing on days 5-7 postoperatively. Daily injections of Compound 48/80 for 5 days prior to operation showed significantly better healing compared to such injections for five days postoperatively. Administration of lupitidine, a long acting histamine H2-receptor antagonist, two times daily starting on the day of 48/80 injection to day 4 after operation did not apparently affect healing. The results indicate that mast cells may be activated during normal wound healing and that a preoperative, pharmacological activation improves healing. Furthermore, histamine does not seem to be of major importance for the beneficial effect of such mast cell activation on connective tissue repair.
Department of Pathology II, University Hospital, Linköping, Sweden.
L
L
Franzén
R
R
Ghassemifar
P
P
Malcherek
D000818:Animals; D003238:Connective Tissue / Q000502:physiology*; D006632:Histamine / Q000378:metabolism; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D008643:Mesentery / Q000293:injuries / Q000378:metabolism / Q000502:physiology*; D011744:Pyrimidinones / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014945:Wound Healing / Q000187:drug effects*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D006635:Histamine H2 Antagonists; D011744:Pyrimidinones; D003189:p-Methoxy-N-methylphenethylamine; C033275:SK&F 93479; D006632:Histamine