[Comparative characteristics of antigenic immunosorbents for isolating antibodies to LDH5].
The authors compare the methods for immobilization of porcine lactate dehydrogenase-5 (LDH5) on various carriers to be used for the preparation of an effective immunosorbent for the isolation of monospecific antibodies. Covalent binding of the enzyme with the carrier via a spacer grouping with the use of glutaraldehyde is advisable for LDH M4-isoform that is characterized by a quaternary structure.
Grebenshchikova
O G
OG
Rashkovetskiĭ
L G
LG
Prozorovskiĭ
V N
VN
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D013552:Swine
D000906:Antibodies; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase
Sravnitel'naia kharakteristika antigennykh immunosorbentov dlia vydeleniia antitel k LDG5.