Point mutation of the neu gene in rat neural tumor RT4-AC cells: suppression of tumorigenicity by s-Myc.
Forced expression of the s-myc gene suppressed the tumorigenicity of rat RT4-AC tumor cells in nude mice, as reported previously. Polymerase chain reaction (PCR) analysis indicated that RT4-AC cells established from a tumor of the peripheral nervous system contain an activated neu gene with a T----A transversion in the transmembrane domain. Synthesis of a protein of 60 kd kd in RT4-AC cells was specifically inhibited by expression of the s-myc gene. These results strongly suggest that s-Myc suppresses the transforming activity of rat neural cells transformed by expression of the activated neu gene, and plays an important role in regulating expression of a cellular gene contributing to cell transformation, such as the gene that encodes the p60 protein.
Lee
S Y
SY
Biophysics Division, National Cancer Center Research Institute, Tokyo.
Sugiyama
A
A
Sueoka
N
N
Kuchino
Y
Y
D000818:Animals; D015152:Blotting, Northern; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc / Q000502:physiology*; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D009154:Mutation; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010524:Peripheral Nervous System Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D009363:Neoplasm Proteins; D012313:RNA; D004247:DNA