PCR-SSO typing for HLA-DRB alleles.
Polymerase chain reaction (PCR) amplification of the second exon of DRB genes from genomic DNA is described. Sequence-specific oligonucleotide (SSO) probes have been designed to allow for the typing of DRB alleles. The methods are described in detail and the results of a trial on both homozygous typing cells and restriction fragment length polymorphism (RFLP)-typed local caucasoid controls are given. The ease of use of this form of DRB typing is emphasized and potential complications are discussed.
Molecular Immunogenetics, Guy's Hospital, London, U.K.
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RW
Vaughan
D000483:Alleles; D001483:Base Sequence; D015342:DNA Probes; D005091:Exons; D006684:HLA-DR Antigens / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D006684:HLA-DR Antigens; D009838:Oligodeoxyribonucleotides