Identification and properties of an alpha-amylase from a strain of Eubacterium sp. isolated from the rat intestinal tract.
1. A bacterial amylase was isolated from the intestinal content of monoxenic rats inoculated with Eubacterium sp. B86. 2. Affinity chromatography on cross-linked starch allowed its separation from rat endogenous amylases. 3. The bacterial enzyme was characterized by its pI, molecular weight and action pattern. It behaves as a typical endo-amylase (alpha-amylase).
Instituto de Qimica y Tecnologia, Facultad de Agronomia, Universitad Central de Venezuela, Maracay.
E P
EP
Delahaye
M J
MJ
Foglietti
C
C
Andrieux
I
I
Chardon-Loriaux
O
O
Szylit
P
P
Raibaud
D000818:Animals; D002850:Chromatography, Gel; D005051:Eubacterium / Q000201:enzymology* / Q000302:isolation & purification; D005856:Germ-Free Life; D006868:Hydrolysis; D007422:Intestines / Q000382:microbiology*; D007525:Isoelectric Focusing; D008297:Male; D008970:Molecular Weight; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D011916:Rats, Inbred F344; D012463:Saliva / Q000201:enzymology*; D000516:alpha-Amylases / Q000032:analysis* / Q000737:chemistry
D000516:alpha-Amylases