Type-common and type-specific monoclonal antibodies to herpes simplex virus types 1 and 2.
Seventeen monoclonal antibodies (Mabs) reacting specifically with the cells infected with herpes simplex viruses type 1 (HSV-1) and type 2 (HSV-2) were characterized by a variety of immunological tests such as radioimmunoprecipitation, immunoblotting and virus-neutralization. The majority of Mabs was directed against glycoprotein B (anti-gB), six reacted with glycoprotein C (anti-gC) and one with glycoprotein G (anti-gG). Six anti-gB Mabs reacted with both types of HSV (anti-gB-1,2), two anti-gB and all the six anti-gC Mabs have been specific for HSV-1 (anti-gB-1 and anti-gC-1). The remaining two anti-gB Mabs and the anti-gG have been specific to HSV-2 (anti-gB-2 and anti-gG). Only three out of the seventeen examined Mabs neutralized the virus.
Institute of Virology, Slovak Academy of Sciences, Bratislava.
M
M
Bystrická
M
M
Vancíková
M
M
Kasalová
J
J
Rajcáni
M
M
Kostál
M
M
Murányiová
K
K
Poláková
G
G
Russ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D002460:Cell Line; D006801:Humans; D006825:Hybridomas / Q000737:chemistry; D008168:Lung / Q000196:embryology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D012703:Serotyping; D018139:Simplexvirus / Q000145:classification* / Q000276:immunology; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014764:Viral Proteins