[Immunoenzyme analysis of insulin antibodies in human blood serum].
Indirect solid-phase enzyme immunoassay (EIA) was used for the titration of antibodies to insulin in human blood sera. The modification suggested by the authors permits assessing the immunoglobulins G and M insulin-binding ability. The technique was optimized for all the stages of analysis and a method for estimating the insulin-binding ability of IgG in the sera diluted 1:80 and of IgM in the sera diluted 1:320 was developed. Fifty blood sera of patients with insulin-dependent diabetes mellitus were analyzed. EIA results were in good correlation with the findings of radioimmunoassay.
Sorokina
N V
NV
Gavrilova
E M
EM
Egorov
A M
AM
Gitel'
E P
EP
Bol'shakova
T D
TD
D003922:Diabetes Mellitus, Type 1 / Q000097:blood; D006801:Humans; D007124:Immunoenzyme Techniques; D007330:Insulin Antibodies / Q000032:analysis*; D011863:Radioimmunoassay
D007330:Insulin Antibodies
Immunofermentnyĭ analiz antitel i insulinu v syvorotke krovi cheloveka.