Anti-idiotypic antibody-induced protection against Clostridium perfringens type D.
A monoclonal antibody (BALB/c mouse) with specificity for a neutralizing epitope on the epsilon-toxin produced by Clostridium perfringens type D was used to raise anti-idiotypic antibodies (anti-Id) in different strains of mice and rabbits. These were purified and used in cross-immunization studies to induce anti-(anti-idiotype). All strains of mice and rabbits immunized with BALB/c-derived anti-Id showed a high-titer antibody response directed towards the active site of the toxin. This protected the animals against toxin challenge and against an oral dose of the vegetative organisms. Animals immunized with other anti-Id preparations showed no specific antibody response and were not protected. Guinea pig peritoneal macrophages have a cell surface receptor for the toxin, and incubation of these cells with BALB/c anti-Id allowed them to survive toxin challenge, indicating that occupation of the receptors by the anti-Id prevented binding by the toxin. In conclusion, we have shown that an internal-image anti-Id preparation will induce protective immunity in syngeneic and xenogeneic animals and furthermore that immunity to a single epitope on the exotoxin is sufficient to protect against the toxin and clinical sequelae evoked by the disease-causing organism itself.
Defence Microbiology Division, Chemical Defence Establishment, Porton Down, Salisbury, Wiltshire, United Kingdom.
D A
DA
Percival
A D
AD
Shuttleworth
E D
ED
Williamson
D C
DC
Kelly
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage* / Q000096:biosynthesis / Q000276:immunology; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000276:immunology; D003015:Clostridium Infections / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D003016:Clostridium perfringens / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D007114:Immunization; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; C039811:Clostridium perfringens epsilon-toxin; D000939:Epitopes