Analyses of surface membrane carbohydrates in parasitic flagellates of the order kinetoplastida using lectins.
Crithidia fasciculata, Leishmania donovani, Leishmania major, Leishmania mexicana amazonensis, Leishmania tropica, Leishmania tarentolae, Trypanosoma sp. from Formosan bats (Tb), Trypanosoma lewisi, Trypanosoma musculi, and different strains of Trypanosoma cruzi (Tc) were cultivated at 27 degrees C in a liquid culture medium. Flagellates harvested from log phase culture were analyzed for their lectin agglutinating characteristics with concanavalin A (Con A), Peanut agglutinin, Ricinus communis agglutinin 120, soybean agglutinin (SBA), Ulex europeus agglutinin (UEA) and wheat germ agglutinin (WGA). Results indicated that all these flagellates might have D-galactose and methyl- alpha-D-manopyranoside on their surface. The presence of L-Fucose, which complexes specifically with UEA, could not be demonstrated on the surface of these flagellates. Results from quantitative comparison of surface molecules of Tb and the Tulahuen strain of Tc suggested that Tb may have more WGA-binding molecules while Tc may have more ConA-binding molecules. Pretreatment of the flagellates with 0.05% trypsin at 37 degrees C for 30 minutes caused some reduction of agglutination titers. Cell agglutination with lectins was completely inhibited or reversed in the presence of the specific lectin-binding monosaccharides.
Chao
D
D
Department of Biology, National Sun Yat-Sen University, Kaohsiung, Taiwan, Republic of China.
Chen
Y A
YA
Liu
J K
JK
Huang
T C
TC
D000371:Agglutination; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis*; D005690:Galactose / Q000032:analysis; D006801:Humans; D037102:Lectins; D007891:Leishmania / Q000032:analysis*; D008766:Methylmannosides / Q000032:analysis; D013194:Staining and Labeling; D014345:Trypanosoma / Q000032:analysis*
D002241:Carbohydrates; D037102:Lectins; D008766:Methylmannosides; C008466:methylmannoside; D005690:Galactose