Isolation of coordinately regulated genes that are expressed in discrete stages of B-cell development.
We have utilized subtractive hybridization to isolate 16 distinct cDNA sequences representing genes expressed in pre-B-cell lines but not myeloma cell or fibroblast lines. These sequences represent RNA transcripts that vary in abundance in pre-B-cell lines from 0.001% to 0.05%. Five of these sequences were not related to any known genes. One was related to but distinct from known myosin regulatory light chain genes and another encoded a protein with lectin domains. Three represented previously identified genes encoding carbonic anhydrase type II, thymosin, and CD2; these genes were not previously known to be specifically expressed in early stages of B-cell development. Other isolated genes corresponded to pre-B-cell-specific or pre-B-cell/B cell-specific genes recently described by others. The isolated cDNA sequences may be divided into two general categories--those representing genes expressed only in the pre-B-cell stage of B-cell development and those expressed in both the pre-B-cell and B-cell stages. The in vivo expression patterns of the identified genes suggest that some function specifically in lymphocytes while others may have roles in additional lineages.
Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
G D
GD
Yancopoulos
E M
EM
Oltz
G
G
Rathbun
J E
JE
Berman
R K
RK
Smith
R D
RD
Lansford
P
P
Rothman
A
A
Okada
G
G
Lee
M
M
Morrow
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005786:Gene Expression Regulation; D015723:Gene Library; D007739:L Cells / Q000276:immunology; D051379:Mice; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D015342:DNA Probes; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA