Specific antisense RNA inhibition of growth hormone production in differentiated rat pituitary tumour cells.
An expression vector that carried an inverted 800 base pair insert of the rat growth hormone (rGH) cDNA downstream of the SV40 promotor was used to transfect two different growth hormone (GH) producing rat pituitary cell strains, GH12C1 and GH3. This resulted in a specific transient inhibition of growth hormone production up to 75 percent in the course of 72 hours. GH synthesis reduction occurred parallel to a decrease of GH cytoplasmic mRNA levels. Levels of beta-actin and guanine nucleotide-binding regulatory protein (G protein) mRNAs were unaltered, but PRL mRNA levels were increased. Transfection with a control vector did not affect GH production.
Institute of Medical Biochemistry, University of Oslo, Norway.
R H
RH
Paulssen
E J
EJ
Paulssen
P
P
Aleström
J O
JO
Gordeladze
K M
KM
Gautvik
D000818:Animals; D015152:Blotting, Northern; D015870:Gene Expression; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics*; D066298:In Vitro Techniques; D010902:Pituitary Gland / Q000502:physiology*; D011388:Prolactin / Q000235:genetics; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D016372:RNA, Antisense; D012333:RNA, Messenger; D012313:RNA; D011388:Prolactin; D013006:Growth Hormone