[Pharmacological studies on leaf of Arctostaphylos uva-ursi (L.) Spreng. I. Combined effect of 50% methanolic extract from Arctostaphylos uva-ursi (L.) Spreng. (bearberry leaf) and prednisolone on immuno-inflammation].
The effect of 50% methanolic extract (U-ext) from Bearberry leaf on immuno-inflammation was studied by contact dermatitis caused by picryl chloride (PC-CD) in mice. The combined effect of U-ext and prednisolone was also investigated by using similar experimental model. When given orally twice immediately before and 16 h after the application of PC-CD, U-ext did not show an inhibitory effect on the swelling induced by PC-CD while it exhibited a significant therapeutic effect at a dose of 100 mg/kg or more once 24 h after the application. When U-ext (p.o.) and prednisolone (s.c.) in a given portion were simultaneously administered, the inhibitory effect was more potent than that of prednisolone alone in both administration immediately before and 16 h after or once 24 h after the application. Arbutin isolated from U-ext also increased the inhibitory effect of prednisolone similarly to that of U-ext. These results suggest that Bearberry leaf possesses a therapeutic effect against immuno-inflammation induced by PC-CD and also increases the inhibitory effect of prednisolone, and its active principle may be arbutin.
Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.
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Kubo
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Ito
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Nakata
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Matsuda
D000818:Animals; D001104:Arbutin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000188:drug therapy; D002199:Capillary Permeability / Q000187:drug effects; D003877:Dermatitis, Contact / Q000188:drug therapy*; D004359:Drug Therapy, Combination; D004487:Edema / Q000188:drug therapy; D006027:Glycosides / Q000627:therapeutic use*; D006636:Histamine Release / Q000187:drug effects; D051379:Mice; D010853:Picryl Chloride; D011239:Prednisolone / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats
D006027:Glycosides; D011239:Prednisolone; D001104:Arbutin; D010853:Picryl Chloride