Toxicity of amphotericin B emulsion to cultured canine kidney cell monolayers.
The effect of amphotericin B in an emulsion formulation on the integrity of monolayers of kidney cells has been studied. Whereas a conventional solubilized amphotericin formulation (Fungizone, Squibb) caused a loss in monolayer integrity at concentrations above 1 microgram mL-1, the emulsion formulation had no measurable effect on confluence at amphotericin concentrations up to 100 micrograms mL-1. The emulsion retained a comparable antifungal activity to that of Fungizone against Saccharomyces cerevisiae in suspension culture. These results parallel the observed erythrocyte lysis data obtained previously using amphotericin B emulsions, and suggest that the emulsion formulation may have a lower toxicity and improved therapeutic potential over existing formulations.
Department of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
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Lamb
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Washington
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Davis
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Denyer
D000666:Amphotericin B / Q000494:pharmacology / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D007668:Kidney / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects
D004655:Emulsions; D000666:Amphotericin B