Microwave-assisted staining procedures in routine histopathology.
The use and practicability of microwave-assisted staining procedures in routine histopathology over more than three years has been evaluated. A domestic microwave oven was used to speed up the following staining procedures: Haematoxylin-Eosin (for frozen sections), Romanowsky-Giemsa, Periodic acid-Schiff (PAS), Ziehl-Neelson, Papanicolaou, Feulgen and Grocott--stain on buffered formalin fixed sections or cytologic smears. These staining procedures can be made highly reproducible providing; (1) Staining vessels are placed in the same position inside the oven; (2) Accurate timing in seconds is observed. Microwave-assisted staining procedures are equal to or even superior to those of the standard methods. Staining times can be reduced to 2%-10% of the conventional staining procedures. The basic staining protocols are presented.
Institute of Pathology, Lutherian Hospital, Oberhausen, FRG.
K
K
Kayser
J
J
Bubenzer
D001399:Azure Stains; D004396:Coloring Agents; D004247:DNA / Q000737:chemistry; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D008872:Microwaves; D010503:Periodic Acid-Schiff Reaction; D012394:Rosaniline Dyes; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*
D001399:Azure Stains; D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D012834:Silver; C006898:carbol-fuchsin solution; D004247:DNA; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin