Regulation of expression of growth arrest-specific genes in mouse fibroblasts.
The suppression of growth arrest-specific (gas) gene expression by serum appeared to be independent of protein synthesis, but expression in resting cells was sensitive to 2-aminopurine, an inhibitor of intracellular protein kinases. Although accumulation of gas gene mRNA was reduced by serum, nuclear transcription of the gas-2, -3, and -5 genes was observed in serum-stimulated cells, indicating that posttranscriptional events may regulate mRNA levels. Growth induction by serum, on the other hand, led to suppression of transcription of the gas-1 gene. Cell cycle regulation and the serum response of gas-1 were lost in ras-transformed cells.
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
C
C
Ciccarelli
L
L
Philipson
V
V
Sorrentino
D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D005786:Gene Expression Regulation; D011905:Genes, ras; D051379:Mice; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D013489:Suppression, Genetic; D014158:Transcription, Genetic
D012333:RNA, Messenger; D012313:RNA