Development of a rotavirus plaque assay using Sephadex G-75.
A rapid, reproducible and easily performed plaque assay is described for use with a variety of rotavirus strains. Plaque formation was induced in MA-104 cells by the use of Sephadex G-75, instead of the traditional agar, and crystalline trypsin in the overlay. Formation of large, discrete, easily read plaques was noted in both human and non-human rotavirus strains.
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Praxis Biologics Inc., Rochester, NY 14623.
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D000818:Animals; D002460:Cell Line; D003911:Dextrans; D005782:Gels; D008253:Macaca mulatta; D012401:Rotavirus / Q000254:growth & development*; D014357:Trypsin; D010948:Viral Plaque Assay / Q000379:methods*
D003911:Dextrans; D005782:Gels; C025614:sephadex; D014357:Trypsin