Evaluation of fingerprinting methods for identification of Helicobacter pylori strains.
Variation amongst strains of Helicobacter pylori was examined by 35S-methionine-labelled protein SDS-PAGE (Radio-PAGE), immunoblot and DNA fingerprinting techniques. These methods allowed discrimination amongst isolates and showed total correlation. Pig and baboon gastric Helicobacter pylori-like organisms were found to be very similar to Helicobacter pylori by both Radio-PAGE and immunoblotting, whereas a Helicobacter mustelae isolate was markedly different. The HindIII restriction endonuclease digest patterns of Helicobacter pylori DNA illustrated the considerable genomic variation of this organism. The Radio-PAGE and immunoblot typing methods both gave precise identification of Helicobacter pylori strains, but Radio-PAGE was found to give higher resolution and represents a standardised universally applicable fingerprinting method for Helicobacter pylori.
Clayton
C L
CL
Department of Medical Microbiology, St. Bartholomew's Hospital Medical School, West Smithfield, London, UK.
Kleanthous
H
H
Dent
J C
JC
McNulty
C A
CA
Tabaqchali
S
S
D000818:Animals; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D005069:Evaluation Studies as Topic; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D015151:Immunoblotting; D010215:Papio / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D013552:Swine / Q000382:microbiology
D004269:DNA, Bacterial; D015247:Deoxyribonuclease HindIII