Light and electron microscopic immunocytochemical demonstration of the coexistence of somatostatin 14- and somatostatin 25-like peptides in endocrine cells of the stomach of Sparus aurata (Teleost).
An immunofluorescence double-staining method colocalized somatostatin 14 (SST 14)- and somatostatin 25 (SST 25)-like immunoreactivities in endocrine cells located in the depth of gastric folds and upper part of the stomach glands of Sparus aurata (gilthead sea bream). An immunogold method identified somatostatin-like peptides in the secretory granules of the previously described Type IV endocrine cells. Appropriate preabsorption controls demonstrated two different granule populations with somatostatin-like immunoreactivity. SST 14-like peptides seemed to be located in the most commonly found granules, which showed a fibrillar content, whereas SST 25-like peptides were identified in more scarce and denser granules.
Department of Cell Biology, Faculty of Biology, University of Murcia, Spain.
M T
MT
Elbal
A
A
García Ayala
B
B
Agulleiro
D000818:Animals; D005753:Gastric Mucosa / Q000378:metabolism*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D010473:Perciformes / Q000378:metabolism*; D011498:Protein Precursors / Q000096:biosynthesis*; D013004:Somatostatin / Q000096:biosynthesis*
D011498:Protein Precursors; D013004:Somatostatin; C026568:somatostatin 25