Mutational analysis of conserved nucleotides in a self-splicing group I intron.
We have constructed all single base substitutions in almost all of the highly conserved residues of the Tetrahymena self-splicing intron. Mutation of highly conserved residues almost invariably leads to loss of enzymatic activity. In many cases, activity could be regained by making additional mutations that restored predicted base-pairings; these second site suppressors in general confirm the secondary structure derived from phylogenetic data. At several positions, our suppression data can be most readily explained by assuming non-Watson-Crick base-pairings. In addition to the requirements imposed by the secondary structure, the sequence of the intron is constrained by "negative interactions", the exclusion of particular nucleotide sequences that would form undesirable secondary structures. A comparison of genetic and phylogenetic data suggests sites that may be involved in tertiary structural interactions.
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
S
S
Couture
A D
AD
Ellington
A S
AS
Gerber
J M
JM
Cherry
J A
JA
Doudna
R
R
Green
M
M
Hanna
U
U
Pace
J
J
Rajagopal
J W
JW
Szostak
D000818:Animals; D001483:Base Sequence; D004252:DNA Mutational Analysis; D007438:Introns; D008969:Molecular Sequence Data; D009152:Mutagenicity Tests; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012313:RNA / Q000737:chemistry; D012326:RNA Splicing; D016337:RNA, Catalytic; D013768:Tetrahymena / Q000235:genetics*
D016337:RNA, Catalytic; D012313:RNA