Molecular definition of interstitial deletions of chromosome 13 in leukemic cells.
Three patients with leukemia and one with a myeloproliferative disorder carried an interstitial deletion of chromosome 13, del(13)(q12q14), in leukemic cells. Proximal and distal breakpoints of the deleted segment were characterized by using DNA restriction fragment length polymorphisms of chromosome 13 supplemented by quantitative densitometry of hybridization signals to determine the copy number of individual loci. Both proximal and distal breakpoints varied between patients, and it is unlikely that a significant hybrid gene was formed by rejoining at the breakpoint junctions. The retinoblastoma gene was encompassed by the deleted segment in all four patients.
Cytogenetic and Molecular Oncology Unit, Christchurch School of Medicine, New Zealand.
C M
CM
Morris
J M
JM
Cochrane
S M
SM
Benjes
P E
PE
Crossen
P H
PH
Fitzgerald
D000368:Aged; D002648:Child; D002872:Chromosome Deletion; D002882:Chromosomes, Human, Pair 13 / Q000648:ultrastructure*; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D016161:Genes, Retinoblastoma; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D055728:Primary Myelofibrosis / Q000235:genetics*
D015342:DNA Probes; D004273:DNA, Neoplasm