Developmental and differential expression of beta amyloid protein precursor mRNAs in mouse brain.
S1 nuclease analysis was used to determine the levels and patterns of three beta amyloid protein precursor (BPP) mRNAs in mouse developmental brain and in primary neuronal and glial cultures. BPP695 mRNA lacking the Kunitz proteinase inhibitor (KPI) domain was detected exclusively in neuronal cultures and increased considerably in late embryonic and early postnatal periods. On the other hand, BPP751 and 770 mRNAs with KPI domain were detected predominantly in astrocyte- and microglia-enriched cultures and increased slightly only in embryonic stages. These results suggest that the product of each BPP mRNA may play a different role in the brain.
Division of Demyelinating Disease and Aging, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
Y
Y
Ohyagi
K
K
Takahashi
M
M
Kamegai
T
T
Tabira
D000544:Alzheimer Disease / Q000235:genetics; D000682:Amyloid / Q000235:genetics*; D016564:Amyloid beta-Protein Precursor; D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry; D002478:Cells, Cultured; D015870:Gene Expression; D051379:Mice; D008807:Mice, Inbred BALB C; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D011498:Protein Precursors / Q000235:genetics*; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*
D000682:Amyloid; D016564:Amyloid beta-Protein Precursor; D011498:Protein Precursors; D012333:RNA, Messenger; D012313:RNA