The analysis of natural killer cell activity by flow cytometry.
A flow cytometric assay was used to detect the lytic and binding capacities of both fresh peripheral blood lymphocytes and purified Leu-19+ natural killer cells against head and neck cancer cell lines. Results demonstrated that natural killer cell-mediated cytotoxicity and effector-target conjugate formation evaluated by flow cytometry was significantly correlated with the standard chromium 51 release assay and the single-cell microscopic assay, respectively. The sorted Leu-19+ natural killer cells demonstrated higher lytic capacity with a corresponding higher binding rate compared with the unsorted peripheral blood lymphocytes and sorted Leu-19- cells. Flow cytometric analysis of natural killer cell activity (a rapid, simple, and quantifiable procedure) is an alternative to the standard chromium 51 release assay.
Department of Head and Neck Surgery, National Institute of Oncology, Budapest, Hungary.
T
T
Racz
P
P
Sacks
N T
NT
Van
D L
DL
Taylor
G
G
Young
S
S
Bugis
H E
HE
Savage
S P
SP
Schantz
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D019002:CD56 Antigen; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D002460:Cell Line; D002860:Chromium Radioisotopes; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000945:Antigens, Differentiation, T-Lymphocyte; D019002:CD56 Antigen; D002860:Chromium Radioisotopes