The analysis of natural killer cell activity by flow cytometry.
A flow cytometric assay was used to detect the lytic and binding capacities of both fresh peripheral blood lymphocytes and purified Leu-19+ natural killer cells against head and neck cancer cell lines. Results demonstrated that natural killer cell-mediated cytotoxicity and effector-target conjugate formation evaluated by flow cytometry was significantly correlated with the standard chromium 51 release assay and the single-cell microscopic assay, respectively. The sorted Leu-19+ natural killer cells demonstrated higher lytic capacity with a corresponding higher binding rate compared with the unsorted peripheral blood lymphocytes and sorted Leu-19- cells. Flow cytometric analysis of natural killer cell activity (a rapid, simple, and quantifiable procedure) is an alternative to the standard chromium 51 release assay.
Racz
T
T
Department of Head and Neck Surgery, National Institute of Oncology, Budapest, Hungary.
Sacks
P
P
Van
N T
NT
Taylor
D L
DL
Young
G
G
Bugis
S
S
Savage
H E
HE
Schantz
S P
SP
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D019002:CD56 Antigen; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D002460:Cell Line; D002860:Chromium Radioisotopes; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000945:Antigens, Differentiation, T-Lymphocyte; D019002:CD56 Antigen; D002860:Chromium Radioisotopes