Definition of murine T helper cell determinants in the major capsid protein of human papillomavirus type 16.
Three murine major histocompatibility complex (MHC) class II-restricted T cell determinants were identified in the major capsid protein L1 of human papillomavirus (HPV) type 16. Peptides derived from HPV-16 L1, which contain putative T cell epitopes located by a predictive algorithm, were synthesized and tested for lymphoproliferative activity by direct immunization, followed by in vitro assay of responses to peptides or recombinant HPV-16 L1. The MHC restriction of the stimulatory peptides was determined using blocking monoclonal antibodies against class II molecules. The responses, which were specific for the priming peptides alone, cross-reacted with recombinant L1 but not with analogous peptides derived from other HPV types.
Department of Biology, University College London, U.K.
D H
DH
Davies
C M
CM
Hill
J B
JB
Rothbard
B M
BM
Chain
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001667:Binding, Competitive; D002213:Capsid / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes; D006801:Humans; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014765:Viral Vaccines
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011994:Recombinant Proteins; D014765:Viral Vaccines