Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines.
The authors successfully established 5 hepatocellular carcinoma cell lines, JHH-1, 2, 4, 5 and 6, derived from hepatitis B surface antigen seronegative patients and one line. JHH-7, from a patient considered to be a hepatitis B virus carrier. In the culture media of any of the JHH cell lines, including JHH-7, hepatitis B surface antigen was not detected by radioimmunoassay. However, in JHH-7, integration of hepatitis B virus DNA was confirmed at two sites on the chromosomes of this line by Southern blot hybridization. In contrast, in other JHH cell lines derived from hepatitis B surface antigen seronegative patients, integration of hepatitis B virus DNA into the chromosomes of cells was not detected. These cell lines will be useful in the investigation of hepatocellular carcinoma development, especially for research into non-A/non-B hepatitis viruses.
Fujise
K
K
First Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.
Nagamori
S
S
Hasumura
S
S
Homma
S
S
Sujino
H
H
Matsuura
T
T
Shimizu
K
K
Niiya
M
M
Kameda
H
H
Fujita
K
K
D000418:Albumins / Q000032:analysis; D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000382:microbiology*; D002460:Cell Line; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D000509:alpha-Fetoproteins / Q000032:analysis
D000418:Albumins; D015342:DNA Probes; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D000509:alpha-Fetoproteins