In vitro and in vivo modification of Neisseria gonorrhoeae lipooligosaccharide epitope structure by sialylation.
After growth of gonococci in the presence of cytidine monophospho-N-acetyl-neuraminic acid (CMP-NANA), their 4.5-kD lipooligosaccharide (LOS) component was increased by approximately 400 daltons, whereas the LOS of strains lacking the 4.5-kD component were unaffected. Expression of mAb-defined epitopes on the 4.5-kD component was decreased on LOS of strains grown in CMP-NANA, and treatment of the LOS with neuraminidase reversed this affect. Gonococci incubated with human PMNs also had decreased expression of the 4.5-kD+ epitopes. A detergent extract of gonococci incorporated radiolabeled NANA in the LOS, suggesting the presence of a sialyltransferase in gonococci. Exogenous sialyltransferases also could use LOS as an acceptor.
Center for Immunochemistry, Veterans Administration Medical Center, San Francisco, California 94121.
R E
RE
Mandrell
A J
AJ
Lesse
J V
JV
Sugai
M
M
Shero
J M
JM
Griffiss
J A
JA
Cole
N J
NJ
Parsons
H
H
Smith
S A
SA
Morse
M A
MA
Apicella
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000276:immunology*; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology* / Q000302:isolation & purification; D008854:Microscopy, Electron; D009344:Neisseria gonorrhoeae / Q000276:immunology* / Q000648:ultrastructure; D009439:Neuraminidase; D009504:Neutrophils / Q000382:microbiology; D011863:Radioimmunoassay; D012794:Sialic Acids / Q000378:metabolism*; D013363:Submandibular Gland / Q000201:enzymology; D013552:Swine
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D012794:Sialic Acids; C023023:lipid-linked oligosaccharides; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; D009439:Neuraminidase