Combined effects of ethanol and manganese on cultured neurons and glia.
Manganese is essential for normal development and activity of the nervous tissue. Mn2+ ions are involved in protein synthesis and may prevent free radical damage. Since it is now established that alcohol degradation may produce free radicals, we studied the effect of Mn2+ on ethanol induced alterations using cultured nerve cells as an experimental model of the central nervous system. Neurons and glial cells were cultured from rat brain cortex; a tumoral rat glial cell line (C6) was also examined. We measured enzymatic markers of nerve cell maturation (enolase, glutamine synthetase) and superoxide dismutase, a scavenger of free radicals; all these enzymes being activated by Mn2+ ions. Only for the glial cell types an alcohol antagonizing effect was found when Mn2+ was combined with ethanol. Neurons were not sensitive to that Mn2+ effect.
Centre de Neurochimie du CNRS, Strasbourg, France.
M
M
Ledig
G
G
Tholey
L
L
Megias-Megias
P
P
Kopp
F
F
Wedler
D000818:Animals; D002478:Cells, Cultured; D000431:Ethanol / Q000494:pharmacology*; D005609:Free Radicals; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
D005609:Free Radicals; D009419:Nerve Tissue Proteins; D000431:Ethanol; D008345:Manganese; D013482:Superoxide Dismutase; D010751:Phosphopyruvate Hydratase; D005974:Glutamate-Ammonia Ligase