Thymocyte maturation following interaction with thymus-derived macrophages.
Murine C57BL/6 thymocytes were cultivated together with syngeneic thymus-derived macrophages (TDM phi) for up to 96 hr to determine whether TDM phi participate in thymocyte maturation. The expression level of H-2b and Thy-1.2 antigens served as thymocyte differentiation surface markers as analyzed by flow cytometry. Indirect immunofluorescent staining profiles of the thymocytes demonstrate a dramatic increase in H-2b expression and a profound decrease in Thy-1.2 expression during cultivation with TDM phi. A similar phenomenon was observed when enriched populations of immature thymocytes were cocultivated with TDM phi. These changes were not observed when thymocytes were cultivated alone or with trypsin-treated TDM phi; neither were they observed when cortisone-resistant thymocytes manifesting mature characteristics were cultivated together with TDM phi. These findings suggest that interaction of thymocytes with TDM phi, involving binding and engulfment, results in the appearance of mature thymocyte subsets.
Lautenberg Center for General & Tumor Immunology, Jerusalem, Israel.
M
M
Zeira
R
R
Gallily
I
I
Stein
H
H
Giloh
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002450:Cell Communication; D002478:Cells, Cultured; D006183:H-2 Antigens / Q000032:analysis; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D006183:H-2 Antigens; D018800:Thy-1 Antigens