Deregulation in trans or c-myc expression in immortalized human urothelial cells and in T24 bladder carcinoma cells.
The expression of a number of cellular oncogenes was investigated in human urothelial cell lines with different in vitro growth properties. Constitutively elevated levels of expression of c-myc RNA were found in Hu609, an immortalized, nontumorigenic cell line that was derived from normal urothelium, and in the bladder carcinoma cell line T24. Potential mechanisms that might underlie deregulation of c-myc expression in these cells were investigated. It was found that the c-myc gene was apparently intact and not amplified in Hu609 and T24. No increased stability of c-myc RNA was detected. A c-myc-CAT fusion construct containing 2.5 kb of normal c-myc 5' sequences showed levels of expression that paralleled the overexpression of the endogenous gene, indicating that the high constitutive levels of c-myc expression were due, at least in part, to alterations in the activities of cellular trans-acting transcriptional regulators.
Laboratory of Environmental Carcinogenesis, Danish Cancer Society, Fibiger Institute, Copenhagen.
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Stacey
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Nielsen
J
J
Skouv
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C
Hansen
H
H
Autrup
D000818:Animals; D004848:Epithelium / Q000737:chemistry; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009857:Oncogenes; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D012313:RNA / Q000378:metabolism; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
D016271:Proto-Oncogene Proteins c-myc; D012313:RNA