CD4+ cells treated with DNA methylation inhibitors induce autologous B cell differentiation.
The DNA methylation inhibitor 5-azacytidine induces autoreactivity in cloned CD4+ T cells, but the functional consequences of this response are unknown. We now report that CD4+ T cells treated with 5-azacytidine respond to autologous antigen-presenting cells and induce autologous B cell differentiation without exogenous antigen or mitogen. This mechanism could play a role in some autoimmune diseases characterized by T cell DNA hypomethylation and polyclonal B cell activation.
Richardson
B C
BC
University of Michigan, Ann Arbor 48109-0531.
Liebling
M R
MR
Hudson
J L
JL
D000120:Acecainide / Q000494:pharmacology; D000917:Antibody Formation / Q000502:physiology; D000938:Antigen-Presenting Cells / Q000276:immunology; D015703:Antigens, CD / Q000096:biosynthesis; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001374:Azacitidine / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D004247:DNA / Q000187:drug effects; D006649:Histocompatibility Antigens / Q000096:biosynthesis; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D019012:Integrin beta1; D017493:Leukocyte Common Antigens; D008745:Methylation; D011342:Procainamide / Q000494:pharmacology
D015703:Antigens, CD; D000943:Antigens, Differentiation; D006649:Histocompatibility Antigens; D019012:Integrin beta1; D004247:DNA; D000120:Acecainide; D017493:Leukocyte Common Antigens; D011342:Procainamide; D001374:Azacitidine; D006918:Hydroxyurea