A monoclonal antibody which identifies an antigen in endothelial cell and epithelial basement membrane.
We isolated a monoclonal antibody which decorates the endothelial cells of normal and lymphatic vessels in formalin-fixed, paraffin-embedded tissue. In addition, the antibody recognizes a previously undescribed substance found in the basement membrane zone and subbasement membrane zone of a variety of epithelial. By ELISA assay, the antigen recognized by this monoclonal antibody is not laminin, type IV collagen or fibronectin. This antibody may be used as a diagnostic tool because it identifies an epitope in neoplasms differentiating towards endothelial cell such as angiosarcoma and Kaposi's sarcoma.
Department of Dermatology, Asahikawa Medical College, Hokkaido, Japan.
S
S
Matsuo
N S
NS
Penneys
J D
JD
Fine
S
S
Gay
M
M
Nadji
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000276:immunology; D006801:Humans; D008208:Lymphatic System / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D000941:Antigens