DNA-RFLP methods and interpretation scheme for HLA-DR and DQ typing.
DNA-restriction fragment length polymorphism (RFLP) typing is a well established and standard technique for identification of HLA-DR and DQ allotypes. We present a detailed review of methods for RFLP analysis and a scheme for interpretation of results. This scheme permits the routine identification of HLA-DR and DQ allotypes using the restriction endonucleases TaqI and either HindIII or MspI.
Molecular Genetics Laboratory, United Kingdom Transplant Service, Bristol.
J L
JL
Bidwell
J D
JD
Bignon
D004247:DNA / Q000235:genetics; D015342:DNA Probes; D006683:HLA-DQ Antigens / Q000145:classification / Q000235:genetics*; D006684:HLA-DR Antigens / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D004247:DNA