Effect of retinoic acid on the infiltration of murine melanoma cells into the type I collagen gel.
Two lines of murine melanoma cells (B16 and Cloudman S91) were cultured on type I collagen gel and the effects of all-trans-retinoic acid on the growth and infiltration into the gel were assayed. In both lines, proliferation and the degree of infiltration were suppressed by the addition of all-trans-retinoic acid. The infiltration-inhibiting effect was expressed very rapidly and was dose-dependent at concentrations ranging from 10(-7) to 10(-5) M of all-trans-retinoic acid. These results suggest the anti-invasive effects of all-trans-retinoic acid on melanoma cells.
Department of Dermatology, Osaka City University Medical School, Japan.
T
T
Kono
M
M
Furukawa
T
T
Tanii
S
S
Taniguchi
N
N
Mizuno
M
M
Ishii
T
T
Hamada
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005782:Gels; D008545:Melanoma / Q000473:pathology*; D051379:Mice; D009361:Neoplasm Invasiveness / Q000473:pathology; D012878:Skin Neoplasms / Q000473:pathology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D005782:Gels; D014212:Tretinoin; D003094:Collagen