Antiinflammatory effects of polypeptide growth factors. Platelet-derived growth factor, epidermal growth factor, and fibroblast growth factor inhibit the cytokine-induced expression of the alternative complement pathway activator factor B in human fibroblasts.
The synthesis of complement components in human fibroblasts is modulated by mediators of inflammation such as cytokines. In particular, interleukin-1 (IL-1) and tumor necrosis factor (TNF) induce time- and dose-dependent increases in the synthesis of complement proteins factor B (FB), C3, and factor H (FH). Polypeptide growth factors are also soluble mediators released during inflammation and able to modulate many fibroblast functions. We have studied the effects of polypeptide growth factors platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and fibroblast growth factor (FGF) on the synthesis of complement proteins in cultured human fibroblasts. PDGF, EGF, and FGF alone did not affect the level of synthesis of any of the complement proteins analyzed, but simultaneous incubation of PDGF, EGF, or FGF with IL-1 and TNF resulted in a dose-dependent inhibition of the cytokine-enhanced expression of FB. Inhibition of FB synthesis was observed between 4 and 8 h of exposure to PDGF and persisted for 4 h after the removal of the growth factor. Analysis of steady-state levels of specific FB mRNA suggested that PDGF-induced inhibition of FB synthesis is mediated at a pretranslational level and that it requires new protein synthesis. The effect of the growth factors was limited to FB, with marginal or no inhibition on the cytokine-enhanced synthesis of C3 and FH, excluding the possibility that the inhibitory effects of PDGF, EGF, and FGF on FB synthesis were due to a negative modulation of the growth factors on cytokine cell membrane receptors. Specific inhibition of cytokine-induced increases in FB synthesis by the growth factors may represent down regulation of the acute inflammatory process, further permitting progression to processes of tissue repair and remodeling. Study of the interactions between cytokines and growth factors in the regulation of synthesis of complement proteins may also provide a system for investigating mechanisms of signal transduction of both polypeptide growth factors and cytokines.
Division of Pulmonary Medicine, St. Louis Children's Hospital, Missouri.
A
A
Circolo
G F
GF
Pierce
Y
Y
Katz
R C
RC
Strunk
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001685:Biological Factors / Q000494:pharmacology*; D015152:Blotting, Northern; D002460:Cell Line; D003167:Complement Activation / Q000187:drug effects*; D011415:Complement Factor B / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D003170:Complement Pathway, Alternative / Q000187:drug effects*; D016207:Cytokines; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000235:genetics*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005347:Fibroblasts; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001685:Biological Factors; D016207:Cytokines; D004792:Enzyme Precursors; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D012313:RNA; D011415:Complement Factor B