The stability, toxicity and effectiveness of unmodified and phosphorothioate antisense oligodeoxynucleotides in Xenopus oocytes and embryos.
The properties of antisense phosphorothioate and unmodified oligodeoxynucleotides have been studied in Xenopus oocytes and embryos. We find that phosphorothioates, like unmodified oligodeoxynucleotides, can degrade Vg1 mRNA in oocytes via an endogenous RNase H-like activity. In oocytes, phosphorothioate oligodeoxynucleotides are more stable than unmodified oligodeoxynucleotides and are more effective in degrading Vg1 mRNA. In embryos, neither unmodified nor phosphorothioate deoxyoligonucleotides were effective in degrading Vg1 message at sub-toxic doses.
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge.
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Woolf
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Jennings
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Melton
D000818:Animals; D001483:Base Sequence; D002470:Cell Survival / Q000187:drug effects; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004722:Endoribonucleases / Q000378:metabolism; D005260:Female; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000378:metabolism* / Q000633:toxicity; D016376:Oligonucleotides, Antisense; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D016914:Ribonuclease H; D013873:Thionucleotides / Q000138:chemical synthesis / Q000378:metabolism* / Q000633:toxicity; D014981:Xenopus
D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D013873:Thionucleotides; D012313:RNA; D004722:Endoribonucleases; D016914:Ribonuclease H