A80915, a new antibiotic complex produced by Streptomyces aculeolatus. Discovery, taxonomy, fermentation, isolation, characterization, and antibacterial evaluation.
New semi-naphthaquinone antibiotics A80915A, B, C, and D were isolated from the fermented broth of Streptomyces aculeolatus A80915 (NRRL 18422). Factors A and C, present in both the broth filtrate and mycelial methanol extract, and factors B and D, found predominantly in the broth filtrate, were recovered by extraction with ethyl acetate. Purification of the individual factors was accomplished by preparative reverse phase high performance liquid chromatograph on C18 bonded silica supports. Factors A through D show antimicrobial activity against Gram-positive aerobic and anaerobic organisms in vitro. Mechanism of action studies demonstrated nearly complete inhibition of macromolecular biosynthesis (protein, RNA, DNA, and cell wall) by A80915 factors A through D. A less highly cyclized semi-naphthaquinone, A80915 factor G, was isolated from the broth of the strain fermented in an alternate medium.
Fukuda
D S
DS
Natural Products Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
Mynderse
J S
JS
Baker
P J
PJ
Berry
D M
DM
Boeck
L D
LD
Yao
R C
RC
Mertz
F P
FP
Nakatsukasa
W M
WM
Mabe
J
J
Ott
J
J
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000187:drug effects; D005285:Fermentation; D051379:Mice; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D009285:Naphthoquinones / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000187:drug effects; D012988:Soil Microbiology; D013203:Staphylococcal Infections / Q000188:drug therapy; D013290:Streptococcal Infections / Q000188:drug therapy; D013302:Streptomyces / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure
C065210:A80915; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009285:Naphthoquinones; D012329:RNA, Bacterial