Induction of cytosine-rich poly(A)+ RNAs in Chlamydomonas reinhardtii by cell wall removal.
RNA accumulation in Chlamydomonas reinhardtii during the course of wall regeneration in protoplasts prepared by treatment of vegetative cells with autolysin was studied. In vitro translation of poly(A)+ RNA isolated from wall-regenerating cells indicated that cell wall removal induced the accumulation of several large RNA transcripts. Some of these induced RNAs were enriched following oligo(dG)-cellulose chromatography (referred to as cytosine-rich). The induced and cytosine-rich poly(A)+ RNAs potentially code for hydroxyproline-rich cell wall glycoproteins (HRGPs). Immunoprecipitation of in vitro translated products with an antiserum against a deglycosylated wall fraction (2BII) confirmed that these RNAs contained species involved in cell wall biosynthesis.
Department of Biological Sciences, University of California, Santa Barbara 93106.
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D015152:Blotting, Northern; D002473:Cell Wall / Q000502:physiology*; D002696:Chlamydomonas / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D003513:Cycloheximide / Q000494:pharmacology; D003596:Cytosine; D007700:Kinetics; D011061:Poly A / Q000096:biosynthesis*; D011068:Poly G; D014176:Protein Biosynthesis; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger; D014415:Tunicamycin / Q000494:pharmacology
D012333:RNA, Messenger; D014415:Tunicamycin; D011061:Poly A; D011068:Poly G; C026628:poly(dG); D012313:RNA; D003596:Cytosine; D003513:Cycloheximide