Cloning and sequence analysis of a cDNA encoding a precursor for rat C-type natriuretic peptide (CNP).
Recent identification of a C-type natriuretic peptide (CNP) in porcine brain strongly suggested that a third member of the natriuretic peptide family still remains to be identified in other species of mammals. A cDNA encoding a precursor for rat CNP was cloned from a rat brain cDNA library and sequenced. The precursor was a 126-residue peptide, carrying a 23-residue signal sequence at the N-terminus and the known porcine CNP-53 sequence at the C-terminus. By RNA blot analysis, rat CNP mRNA was found to be expressed exclusively in the brain, implying that CNP may function in the central nervous system as a neuropeptide.
Department of Biochemistry, Miyazaki Medical College, Japan.
M
M
Kojima
N
N
Minamino
K
K
Kangawa
H
H
Matsuo
D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D011498:Protein Precursors; D012313:RNA; D009320:Atrial Natriuretic Factor; D004247:DNA