Immortalization of brain capillary endothelial cells with maintenance of structural characteristics of the blood-brain barrier endothelium.
Early passage bovine brain capillary endothelial cells were immortalized by transfection with the plasmid pSV3 neo. Cells from one clone, SV-BEC, expressed nuclear SV 40 large T antigen, displayed a contact-inhibited and anchorage-dependent proliferation, and a high sensitivity to the addition of exogenous basic fibroblast growth factor. SV-BEC cells are morphologically unaltered and express typical markers of endothelial cells: Factor VIII-related antigen, angiotensin-converting enzyme and Griffonia simplicifolia agglutinin binding site. Endothelium like immunoreactivity was detected in the conditioned medium from these cells. Moreover, SV-BECs present numerous intercellular tight junctions characteristic of the blood-brain barrier and possess functional beta 1- and beta 2-adrenergic receptors, as observed on isolated bovine brain capillaries.
Laboratoire d'Immuno-Pharmacologie Moléculaire, C.N.R.S., Université Paris VII, Institut Cochin de Génétique Moléculaire, (IGGM), Paris, France.
O
O
Durieu-Trautmann
N
N
Foignant-Chaverot
J
J
Perdomo
P
P
Gounon
A D
AD
Strosberg
P O
PO
Couraud
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D015415:Biomarkers; D001812:Blood-Brain Barrier; D002196:Capillaries; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D046508:Culture Techniques / Q000379:methods; D000242:Cyclic AMP / Q000378:metabolism; D016232:Endothelins / Q000032:analysis; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D007365:Intercellular Junctions / Q000502:physiology / Q000648:ultrastructure; D007545:Isoproterenol / Q000494:pharmacology; D008854:Microscopy, Electron; D010957:Plasmids; D011412:Propanolamines / Q000378:metabolism; D011863:Radioimmunoassay; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D000319:Adrenergic beta-Antagonists; D000952:Antigens, Polyomavirus Transforming; D015415:Biomarkers; D016232:Endothelins; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; D016222:Fibroblast Growth Factor 2; D000242:Cyclic AMP; D007545:Isoproterenol; C033394:CGP 12177