Changes in the expression of class I major histocompatibility complex antigen RNA induced by interferon in rat hepatoma cells.
The rat hepatoma cell line 17X was studied to determine if it expressed major histocompatibility complex (MHC) class I antigen RNA and to see if interferon treatment would affect its expression. Under normal cell culture conditions, MHC class I RNA in 17X hepatoma cells is virtually undetectable. Administration of rat interferon at a concentration of 1000 units/ml for 48 h to 17X cells in culture resulted in the appearance of detectable levels of RNA for MHC class I antigens. The interferon-induced increase in class I RNA was accompanied by de novo synthesis of immunoprecipitable, metabolically radiolabeled class I antigens in the 17X hepatoma cells.
Scott
R J
RJ
Department of Pathology and Laboratory Medicine, University of Texas Medical School, Houston 77225.
Hammons
K V
KV
Hunt
J M
JM
D000818:Animals; D001483:Base Sequence; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D007372:Interferons / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D011233:Precipitin Tests; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000187:drug effects*; D051381:Rats; D014407:Tumor Cells, Cultured
D015395:Histocompatibility Antigens Class I; D015345:Oligonucleotide Probes; D012334:RNA, Neoplasm; D007372:Interferons