The potential to express or suppress human interleukin-2 and interferon-gamma genes is not restricted to distinct cell subsets.
Cell surface markers CD4, CD8, Leu8 and Leu15 (CD11) were used to separate human lymphoid cell subsets with monoclonal antibody-coated immunomagnetic beads. We show that each of these subsets is able to suppress the induction of IL-2 and IFN-gamma genes effectively. This is manifested by a pronounced superinduction of IL-2 and IFN-gamma mRNA, as well as IFN-gamma protein, in cell populations depleted of one of these subsets. Co-culture of cell subsets with total cell populations or depleted ones, on the other hand, leads to severe inhibition of expression of these genes. In these experiments, cells in suppressor subsets exhibit little, if any, expression of IL-2 and IFN-gamma genes. By contrast, depending on donor and lymphoid tissue examined (tonsils or peripheral blood mononuclear cells), CD4, CD8, Leu8, and Leu15 cell subsets are also able to express IL-2 or IFN-gamma genes to high levels. Moreover, in Leu8+ cells that do not express the IFN-gamma gene, extensive expression of both mRNA and protein can be elicited by inhibiting the activation of suppressor cells with gamma-irradiation before induction. These results support the concept that the potential to express or suppress human IL-2 and IFN-gamma genes is not restricted to distinct cell subsets. Suppression or expression can be elicited in cells carrying a given surface marker, depending on the state of the immune system in a lymphoid tissue.
Ketzinel
M
M
Department of Molecular Virology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Arad
G
G
Tal
C
C
Schlesinger
M
M
Nussinovich
R
R
Reshef
A
A
Sayar
D
D
Gerez
L
L
Kaempfer
R
R
D015703:Antigens, CD / Q000032:analysis; D000943:Antigens, Differentiation / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D018845:CD11 Antigens; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D016827:CD8 Antigens; D015815:Cell Adhesion Molecules / Q000032:analysis; D002469:Cell Separation / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D007376:Interleukin-2 / Q000235:genetics*; D019041:L-Selectin; D016131:Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis
D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D018845:CD11 Antigens; D016827:CD8 Antigens; D015815:Cell Adhesion Molecules; D007376:Interleukin-2; D012333:RNA, Messenger; D019041:L-Selectin; D007371:Interferon-gamma