Expression in cultured human neuroblastoma cells of epitopes associated with affected neurons in Alzheimer's disease.
Of three human neuroblastoma lines tested, IMR32K (and IMR32 parental line) was the only cell line that, after its exposure to a differentiation medium, consistently developed materials recognized immunocytochemically by a panel of antibodies against paired helical filaments (PHF). Ultrastructurally, these cells accumulated, at their perikarya and neuritic extensions, spatially discrete arrays of fibrils, which occasionally occurred in twisted pairs. When these fibrillar structures appeared as paired helices, they exhibited dimensions and configurations reminiscent of PHF found in affected Alzheimer neurons, although less compact. Immunoelectron microscope examinations of the fibrillar structures in these neuroblastoma cells with one of these anti-PHF immunoprobes revealed that only subsets of fibrillar structures that appeared thickened or aggregated to form bundles were selectively immunolabeled. Cultures of these immortal neuroblastoma lines may provide a convenient model for studying aspects of PHF formation that are hard to examine in Alzheimer brain obtained at autopsy.
Altschul Laboratory for Dementia Research, Burke Rehabilitation Center, Cornell University Medical College, White Plains, New York 10605.
L W
LW
Ko
K F
KF
Sheu
O
O
Young
H
H
Thaler
J P
JP
Blass
D000544:Alzheimer Disease / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002454:Cell Differentiation; D000939:Epitopes; D006801:Humans; D007124:Immunoenzyme Techniques; D007382:Intermediate Filaments / Q000276:immunology; D008854:Microscopy, Electron; D009447:Neuroblastoma / Q000276:immunology* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000939:Epitopes