Cloning and characterization of the ribosomal RNA gene repeat from Ostertagia ostertagi.
Clones of the rRNA genes of Ostertagia ostertagi were selected from a library prepared from the genomic DNA of adult worms of strain LA-2. A 13.4-kb insert in a clone, lambda OOR78, is comprised of one complete 7.5-kb rDNA unit and portions of two adjacent units. The rDNA unit is directly repeated in a head-to-tail fashion and represents approximately 0.9% of the total genomic DNA. This repeating unit appears to be the only long tandemly repeated sequence in the genome. Restriction enzyme recognition sites in the rDNAs of four strains of O. ostertagi were fully conserved with the exception of one PstI site present in the large rRNA gene which was absent from a proportion of the genes of the LA-2 strain. The rDNA of O. ostertagi is more similar to that of Caenorhabditis elegans in unit length and arrangement than to parasitic helminths previously examined.
Department of Infectious Diseases, University of Florida, Gainesville 32611-0633.
J B
JB
Dame
C A
CA
Yowell
C H
CH
Courtney
W G
WG
Lindgren
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D005819:Genetic Markers; D006801:Humans; D007814:Larva / Q000254:growth & development; D010028:Ostertagia / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004275:DNA, Ribosomal; D005819:Genetic Markers