Methylation of DNA in megaloblastic anaemia.
Methylation of cytosine residues in DNA samples, collected before and serially after cobalamin treatment from patients with cobalamin deficiency, was studied using restriction endonucleases Hpa II and Msp I and an epsilon globin gene probe. There was no evidence of hypomethylation in any of the samples. It was concluded that although hypomethylation of metabolites such as choline occurs, that of DNA is preserved in megaloblastic anaemia.
Haematology Section, MRC Clinical Research Centre, Northwick Park Hospital, Harrow, Middlesex.
J
J
Perry
I
I
Chanarin
R
R
Deacon
M
M
Lumb
D000748:Anemia, Macrocytic / Q000201:enzymology*; D000749:Anemia, Megaloblastic / Q000188:drug therapy / Q000201:enzymology*; D015139:Blotting, Southern; D004247:DNA / Q000378:metabolism*; D015342:DNA Probes / Q000032:analysis; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific / Q000032:analysis; D006801:Humans; D008745:Methylation; D014805:Vitamin B 12 / Q000627:therapeutic use
D015342:DNA Probes; D004247:DNA; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D014805:Vitamin B 12