Stathmin phosphorylation patterns discriminate between distinct transduction pathways of human T lymphocyte activation through CD2 triggering.
CD2 triggering of human T lymphocyte activation has been associated with the activation of different interacting protein kinases, including protein kinase C (PKC). However the precise roles of its phosphorylated substrates are still unknown. We show here that PKC-dependent and -independent pathways are responsible for the CD2-induced phosphorylation of stathmin, a ubiquitous soluble phosphoprotein, most likely acting as a general intracellular relay integrating various second messenger pathways. The phosphorylated variants of stathmin provide a fingerprint reflecting the second messenger pathway(s) stimulated. The respective roles of both PKC and stathmin in the regulation of T lymphocyte proliferation are discussed.
CNRS URA 625, C.E.R.V.I., C.H. Pitié-Salpètrière, Paris, France.
S
S
le Gouvello
H
H
Chneiweiss
N
N
Tarantino
P
P
Debre
A
A
Sobel
D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D018801:CD2 Antigens; D006801:Humans; D008213:Lymphocyte Activation; D008868:Microtubule Proteins; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D011971:Receptors, Immunologic / Q000502:physiology*; D015398:Signal Transduction; D050777:Stathmin; D013601:T-Lymphocytes / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D008868:Microtubule Proteins; D010750:Phosphoproteins; D011971:Receptors, Immunologic; C493995:STMN1 protein, human; D050777:Stathmin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate