Purification of monoclonal antibodies by complex-displacement chromatography on CM-cellulose.
A single high-capacity chromatographic procedure was used to purify a monoclonal antibody from ascites fluid. A new displacement procedure involving a displacer protein complex is described. The scale-up from 1 ml to 450 ml of ascites sample was easily accomplished with this complex-displacement system. A monoclonal antibody recovery of 79% was measured for the largest-scale purification.
Bio-Fractionations, Logan, UT 84321.
A R
AR
Torres
E A
EA
Peterson
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001202:Ascitic Fluid / Q000032:analysis; D002482:Cellulose; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003911:Dextrans; D051379:Mice
D000911:Antibodies, Monoclonal; D003911:Dextrans; D002482:Cellulose; C014392:carboxymethyl dextran