Species-specific Mn2+/Mg2+ antiport from Mg2(+)-loaded erythrocytes.
Mg2(+)-loaded rat erythrocytes performed Mn2+/Mg2+ antiport, which was nonspecifically stimulated by anions and cations. Mn2+/Mg2+ antiport was shown to operate via the Na+/Mg2+ antiporter because extracellular Na+ and Mn2+ inhibited the intracellular uptake of each other's ions competitively. Furthermore, Mn2+/Mg2+ antiport and Na+/Mg2+ antiport were identically inhibited by various amiloride derivatives. Na+/Mg2+ antiport of chicken and human erythrocytes cannot perform Mn2+/Mg2+ antiport although chicken erythrocytes took up more Mn2+ than rat erythrocytes.
Institute of Molecular Biology and Biochemistry, Free University of Berlin, FRG.
T
T
Günther
J
J
Vormann
E J
EJ
Cragoe
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D000838:Anions; D001692:Biological Transport / Q000187:drug effects; D002412:Cations; D002645:Chickens; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007473:Ion Channels; D008274:Magnesium / Q000097:blood*; D008345:Manganese / Q000097:blood* / Q000494:pharmacology; D011189:Potassium Chloride; D051381:Rats; D012964:Sodium / Q000097:blood / Q000494:pharmacology; D012965:Sodium Chloride; D013045:Species Specificity; D013395:Sucrose
D000838:Anions; D002412:Cations; D007473:Ion Channels; D008345:Manganese; D012965:Sodium Chloride; D013395:Sucrose; D011189:Potassium Chloride; D000584:Amiloride; D012964:Sodium; D008274:Magnesium