Effects of aflatoxin on lymphoid cells of weanling rat.
Aflatoxin (AF), the hepatocarcinogenic food contaminant produced by the Aspergillus flavus group of fungi, is known to interact with various vital processes, including the immune function. Effects of long-term treatment of three dose levels of aflatoxin B1 (AFB1) on lymphoid cells of weanling rats were studied. AFB1 treatment caused a reduction in body weight gain, significantly (P less than 0.01) at the 700 microgram level. There was also a significant decrease in the weight of spleen and thymus in AFB1-treated animals in comparison to control. Similarly, AFB1 depleted cell populations of thymus and bone marrow and WBC and RBC counts. There was a marked reduction in the population and phagocytic capacity of macrophages due to AFB1 administration at dose levels of 350 and 700 micrograms kg-1 body weight. Macromolecular synthesis of DNA, RNA and protein in macrophages was affected, as there was significant inhibition in the incorporation of [3H]-thymidine, [3H]-uridine and [3H]-leucine. The hampered functioning of macrophages may be due to the cytotoxic action of AFB1.
Raisuddin
Preventive Toxicology Division, Industrial Toxicology Research Centre, Lucknow, India.
Singh
K P
KP
Zaidi
S I
SI
Saxena
A K
AK
Ray
P K
PK
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D001772:Blood Cell Count; D001835:Body Weight / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D008214:Lymphocytes / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D000348:Aflatoxins; D012313:RNA; D004247:DNA; D016604:Aflatoxin B1