Regulation of stimulus-induced calcium transport pathways in human T (Jurkat) lymphoblasts.
In human T (Jurkat) lymphoblasts we have studied the calcium signals induced by monoclonal antibodies reacting with the T-cell antigen receptor complex (TCR and CD3). Jurkat cells were preloaded with the fluorescent calcium indicator Indo-1 and the stimulus-induced rise in cytoplasmic free calcium concn was followed in the absence or in the presence of external calcium. The technique allowed the separate investigation of the intracellular calcium release and the external calcium influx processes. The changes in the membrane potential of Jurkat cells were followed simultaneously by using fluorescent indicators. We found that the activation of protein kinase C by phorbol ester (PMA) or by the permeable diacyl glycerol, DiC8, rapidly eliminated the calcium signal, independently of the presence or absence of external calcium, while these treatments did not appreciably change the membrane potential. In contrast, cell membrane depolarization achieved by various treatments selectively blocked the stimulus-induced calcium influx, while did not affect stimulus-induced calcium release from internal stores. The magnitude of the stimulus-induced calcium influx was found to be largely independent of the external calcium concns between about 2-2500 microM. It is demonstrated that the inhibitory effect of membrane depolarization on calcium influx is not simply due to the reduction of the inward calcium gradient under these conditions. These observations indicate a significant down-regulation of the stimulus-induced calcium signal by protein kinase C activation and a selective inhibition of the receptor-operated calcium channels by membrane depolarization.
National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
B
B
Sarkadi
A
A
Tordai
M
M
Müller
G
G
Gárdos
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000911:Antibodies, Monoclonal; D052160:Benzothiazoles; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002232:Carbocyanines / Q000494:pharmacology; D002460:Cell Line; D004075:Diglycerides / Q000494:pharmacology; D006096:Gramicidin / Q000494:pharmacology; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D016853:Muromonab-CD3; D011493:Protein Kinase C / Q000187:drug effects / Q000502:physiology; D013601:T-Lymphocytes / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D052160:Benzothiazoles; D002232:Carbocyanines; D004075:Diglycerides; D016853:Muromonab-CD3; C044295:1,2-dioctanoylglycerol; D006096:Gramicidin; C012944:3,3'-dipropyl-2,2'-thiadicarbocyanine; D002731:4-Chloromercuribenzenesulfonate; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium