Quantification of free alpha 2-macroglobulin and alpha 2-macroglobulin-protease complexes by a novel ELISA system based on streptococcal alpha 2-macroglobulin receptors.
An ELISA test system has been developed for the quantification of the two distinct forms of the proteinase inhibitor alpha 2-macroglobulin (alpha 2M): (i) free alpha 2M (functionally active), which is the electrophoretically slow form (alpha 2 MS), and (ii) the alpha 2 M-proteinase complex (functionally inactive), which is the electrophoretically fast form of alpha 2 M (alpha 2 MF). Discrimination between the two types of alpha 2 M was achieved using extracts of the two independent streptococcal strains, M1 and Sc1, which express receptors for alpha 2 MS and alpha 2 MF, respectively, in combination with a monoclonal antibody specific for alpha 2 M. The assay system described is easy and reliable and permits quantitation of alpha 2 MS and alpha 2 MF in complex biological samples such as plasma and cutaneous suction blister fluid.
Justus
C W
CW
MPG Klinische Forschungsgruppe für Blutgerinnung und Thrombose, Giessen, F.R.G.
Müller
H P
HP
Simon
M M
MM
Kramer
M D
MD
D001768:Blister / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008744:Methylamines / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000032:analysis*; D011971:Receptors, Immunologic / Q000378:metabolism*; D012867:Skin / Q000032:analysis; D013291:Streptococcus / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000032:analysis*
D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008744:Methylamines; D011971:Receptors, Immunologic; D000511:alpha-Macroglobulins; C027451:methylamine; D010447:Peptide Hydrolases