[Molecular epidemiological markers of Legionella pneumophila in the Valencian Community (II). Restriction enzyme profiles of chromosomal DNA].
Restriction enzyme profiles of chromosomal DNA obtained from 96 Legionella pneumophila strains isolated from environmental and clinical isolates and digested with Hind III have been analyzed. A method to obtain chromosomal DNA to be use in restriction studies and its electrophoretic analysis have been developed. Studying the fragments showing higher differences when comparing the profiles obtained, 5 subtypes are defined and correlated with the isolation areas. There are similar restriction profiles independent of the plasmid contents.
Departamento de Microbiología, Facultad de Medicina, Hospital Clínico Universitario, Valencia.
C
C
Muñoz
V
V
Catalán
R
R
Borrás
J
J
García-de-Lomas
D015415:Biomarkers; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D007875:Legionella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007877:Legionnaires' Disease / Q000453:epidemiology / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D013030:Spain / Q000453:epidemiology; D014871:Water Microbiology
D015415:Biomarkers; D004269:DNA, Bacterial
Marcadores epidemiológicos moleculares de Legionella pneumophila en la Comunidad Valenciana (II). Perfiles de restricción enzimática del ADN cromosómico.