Synthetic peptides to identify antigenic determinants on Epstein-Barr virus gp350/220.
We synthesized three peptides, MA1 - Thr19-Val28(+Tyr) -, MA2 - Ser807-Ala816-, and MA3-Ser718-Glu729(+Tyr) from the sequence of Epstein-Barr virus gp350/220 and immunized rabbits with these peptides. Rabbit antisera to the peptides had antipeptide radioimmunoassay titers of 1:400 for anti-MA1, 1:200 for anti-MA2, and 1:1600 for anti-MA3. The anti-MA1 serum recognized gp350/220 in Western blotting to SDS-electrophoresed proteins from 12-O-tetradecanoylphorbol-13-acetate- and n-butyrate-treated B95-8 cells, but anti-MA2 and MA3 sera did not. None of the sera reacted with gp350/220 by membrane or cytoplasmic immunofluorescence or by immunoprecipitation of Triton X-100 solubilized proteins.
Bertoni
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Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655.
Kostyal
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Reisert
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Humphreys
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Sairenji
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D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology*; D009842:Oligopeptides / Q000276:immunology*; D011233:Precipitin Tests; D011863:Radioimmunoassay; D014763:Viral Matrix Proteins
D000956:Antigens, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D000939:Epitopes; D009842:Oligopeptides; D014763:Viral Matrix Proteins