Time dependent uptake of (-)125I-iodocyanopindolol but not (-)125I-iodopindolol by murine splenic lymphocytes.
Time dependent uptake of (-)125I-iodocyanopindolol by intact murine splenocytes was investigated in an attempt to prevent its interference in receptor binding experiments. Four approaches were tried in an attempt to eliminate this uptake; including the use of the permeable amine chloroquine; the inclusion of catechol, chloroquine, and phentolamine into the binding assay; the addition of methylamine to the assay; and the elimination of ascorbate from the assay. None of these approaches completely eliminated the uptake of ICYP over time. Comparative studies with (-)125I-iodopindolol showed this ligand did not have time dependent uptake and may provide for less problematic intact cell receptor assays.
Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0613.
C C
CC
Johnson
B A
BA
Fuchs
D000319:Adrenergic beta-Antagonists / Q000378:metabolism*; D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D010869:Pindolol / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D013154:Spleen / Q000378:metabolism
D000319:Adrenergic beta-Antagonists; D007457:Iodine Radioisotopes; C029247:3-iodopindolol; D020120:Iodocyanopindolol; D010869:Pindolol