Binding of transforming protein, P47gag-crk, to a broad range of phosphotyrosine-containing proteins.
Although the oncogene product of CT10 virus, P47gag-crk, does not itself phosphorylate proteins at tyrosine residues, it elevates phosphotyrosine in transformed cells. The P47gag-crk oncoprotein contains SH2 and SH3 domains, which are conserved in several proteins involved in signal transduction, including nonreceptor tyrosine kinases. P47gag-crk bound in vitro to phosphotyrosine-containing proteins from crk-transformed cells and from cells transformed by oncogenic tyrosine kinases. The association between P47gag-crk and p60v-src, a phosphotyrosine-containing protein, was abolished by dephosphorylation of p60v-src. This suggests that the SH2 and SH3 regions function to regulate protein interactions in a phosphotyrosine-dependent manner.
Matsuda
M
M
Rockefeller University, New York, NY 10021.
Mayer
B J
BJ
Fukui
Y
Y
Hanafusa
H
H
D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D050736:Oncogene Protein v-crk; D010766:Phosphorylation; D019000:Phosphotyrosine; D011233:Precipitin Tests; D011485:Protein Binding; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D012191:Retroviridae Proteins / Q000378:metabolism*; D015398:Signal Transduction; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D050736:Oncogene Protein v-crk; D011518:Proto-Oncogene Proteins; D012191:Retroviridae Proteins; C060731:p47 (gag-crk) protein, Avian sarcoma virus; D019000:Phosphotyrosine; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases