In vivo effects of recombinant granulocyte-colony stimulating factor in athymic nude mice.
The possible role of T lymphocytes in in vivo regulation of haemopoiesis by recombinant human granulocyte-colony stimulating factor (rhG-CSF) was evaluated. Athymic nude (nu/nu) mice and their normal (+/+) littermates were injected subcutaneously twice daily with 100 micrograms/kg per day of rhG-CSF for 5 days. Such parameters as number of neutrophils in blood, spleen weight and cellularity, bone marrow cellularity, and number of stem and progenitor cells (colony forming units in spleen [CFU-S], mix colony forming cells [Mix-CFC], granulocyte-macrophage colony forming cells [GM-CFC] in bone marrow and spleen were evaluated. Some effects of the rhG-CSF treatment were similar in nu/nu and +/+ mice. Others, however, were to some extent different in the two groups of animals. It is concluded that T lymphocytes may be partially responsible for some of the effects of rhG-CSF in vivo activity.
Division of Radiation Hazards, National Institute of Radiological Sciences, Chiba, Japan.
Z
Z
Pojda
Y
Y
Aoki
A
A
Tsuboi
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007958:Leukocyte Count / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009504:Neutrophils / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D011994:Recombinant Proteins; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects*
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor