Immunohistochemistry.
The use of the ABC technique, in which an avidin-biotin interaction is used for immunohistochemical studies, is a simple and straightforward procedure. All required reagents are commercially available. The main advantages of this technique are its sensitivity, specificity, and flexibility. It is crucial for the success of this staining procedure that the antibodies, the type of tissues, and the fixatives that are used be selected properly. The method can easily be adapted for electron microscopy, flow cytometry, in situ hybridization, Western blotting, and ELISA.
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D000818:Animals; D000906:Antibodies; D001360:Avidin; D001710:Biotin; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry / Q000379:methods*; D007202:Indicators and Reagents; D037102:Lectins; D013194:Staining and Labeling
D000906:Antibodies; D007202:Indicators and Reagents; D037102:Lectins; D001360:Avidin; D001710:Biotin