Assay of complement activation in mouse serum by ELISA.
We describe a simple, sensitive and reproducible ELISA assay for complement activation in mouse serum, based on measurement of the opsonization of purified, heat-aggregated mouse gamma-globulin bound to the solid phase. This assay measures mainly classical pathway activation, and we used it to show significant differences in complement activation in sera from mice of various autoimmune, immunodeficient and immunologically normal strains. This assay has the advantage over existing methods of requiring minimal volumes of serum, and of avoiding problems of species incompatibility.
Institute of Neurological Sciences, Southern General Hospital, Glasgow, U.K.
L J
LJ
Morrison
P O
PO
Behan
W M
WM
Behan
D000818:Animals; D003167:Complement Activation / Q000187:drug effects / Q000276:immunology; D003176:Complement C3 / Q000276:immunology; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D008722:Methods; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D013045:Species Specificity; D005719:gamma-Globulins / Q000276:immunology
D003176:Complement C3; D005719:gamma-Globulins; D004533:Egtazic Acid; D004492:Edetic Acid