Primary immune responses of selected small mammal species to heterologous erythrocytes.
1. We surveyed the primary humoral immune responsiveness of six small mammal species (Peromyscus leucopus, Microtus pinetorum, Perognathus hispidus, Neotoma floridana, Onychomys leucogaster, Mus musculus) collected from wild populations in central Oklahoma using sheep red blood cells (SRBC) as the immunogen and a splenic plaque-forming cell (PFC) assay. 2. Individuals within each wild species examined produced antibodies to a single intraperitoneal injection of SRBC, however, considerable interspecific and intraspecific variation in responsiveness was indicated. 3. Overall, primary immune responsiveness varied from 0 to 5013 PFC/10(6) cells. Spleen weights, total splenic nucleated cell yields, PFC/spleen, PFC/mg spleen, and PFC/10(6) cells were significantly different among species. 4. Mean cell yield in M. pinetorum was greater than in P. leucopus and CD-1 laboratory mice (included as positive controls). Number of PFC/10(6) cells was greater in CD-1 laboratory mice than P. hispidus and P. leucopus. The coefficient of variation for PFC/10(6) cells in CD-1 laboratory mice was 38% compared to 109, 129, and 56% for M. pinetorum, P. hispidus, and P. leucopus, respectively. 5. Interspecific and intraspecific differences among wild species may be a reflection of disparate life histories and other environmental selection pressures.
Department of Zoology, Oklahoma State University, Stillwater 74078.
R L
RL
Lochmiller
M R
MR
Vestey
S T
ST
McMurry
D000818:Animals; D000917:Antibody Formation; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D008322:Mammals / Q000276:immunology*; D012756:Sheep / Q000276:immunology; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014183:Transplantation, Heterologous