Chemiluminescence response and arachidonic acid metabolism of macrophages induced by gamma-hexachlorocyclohexane (lindane).
The influence of gamma-hexachlorocyclohexane (gamma-HCCH) on arachidonic acid (AA) metabolism and oxygen metabolite production was investigated on mouse peritoneal macrophages gamma-HCCH stimulated 6KPGF1 alpha, PGE2, LTC4, LTB4 and HETEs production and increased the luminol-dependent chemiluminescence (CL). Lindane acted synergistically with phorbol ester on prostaglandins-leukotrienes (PGs-LTs) and CL production. Similar stimulation of CL and PGs-LTs production was found after challenge by the calcium ionophore A23187. The implication of calcium mobilization in lindane effects was proposed.
INSERM U87, Université Paul Sabatier, Toulouse, France.
M F
MF
Forgue
E
E
Pinelli
M
M
Beraud
M C
MC
Souqual
B
B
Pipy
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D004357:Drug Synergism; D001556:Hexachlorocyclohexane / Q000494:pharmacology*; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D011453:Prostaglandins / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D001095:Arachidonic Acids; D011453:Prostaglandins; D016718:Arachidonic Acid; D001556:Hexachlorocyclohexane; D013755:Tetradecanoylphorbol Acetate