Cytogenetic characterization of 20 lymphoblastoid lines derived from human individuals differing in bleomycin sensitivity.
Forty lymphoblastoid (lymphoid) lines were established from 42 volunteer blood donors, including healthy individuals and patients with head and neck carcinomas. Each peripheral blood sample was split into two portions, one for the establishment of a lymphoid line and the other for short-term culture, which was used to estimate bleomycin sensitivity by cytogenetic procedures. Twenty lymphoid lines were selected at random to compare bleomycin sensitivity with data obtained from short-term lymphocyte cultures. In each set, bleomycin sensitivity of lymphoid cells was similar to that of the lymphocytes. The lymphoid lines, which can be propagated for an unlimited supply of relatively homogeneous cellular material, will be useful for a variety of future investigations.
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
T C
TC
Hsu
E J
EJ
Shillitoe
L M
LM
Cherry
Q
Q
Lin
S P
SP
Schantz
C
C
Furlong
D001761:Bleomycin / Q000633:toxicity*; D001769:Blood; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002842:Chromatids / Q000187:drug effects / Q000648:ultrastructure; D003582:Cytogenetics; D004249:DNA Damage; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects
D001761:Bleomycin