Experiments on the use of DAMP to study retina and cultured neurons.
Immunocytochemical localization of DAMP, a reagent used to detect low pH intracellular compartments, was studied in cultured neurons from rat hippocampus and in frog retinas. We find that DAMP is more sharply localized and that the immunocytochemical reaction is stronger when horseradish peroxidase or other proteins are included in the medium used to administer DAMP to the cells. A likely explanation is that the proteins enter acidified endocytic compartments and there provide sites to which DAMP molecules can be attached during fixation.
Department of Biological Sciences, Columbia University, New York, New York 10027.
E
E
Augenbraun
D
D
Sulzer
S
S
Rayport
W
W
Setlik
E
E
Holtzman
D000818:Animals; D002478:Cells, Cultured; D004136:Dinitrobenzenes; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000648:ultrastructure*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D007202:Indicators and Reagents; D009474:Neurons / Q000648:ultrastructure*; D009499:Neutral Red; D011894:Rana pipiens; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000648:ultrastructure*; D013194:Staining and Labeling
D004136:Dinitrobenzenes; D007202:Indicators and Reagents; D009499:Neutral Red; C042432:3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine; D006735:Horseradish Peroxidase