Use of terpene-based products, with and without anhydrous alcohol, as clearing agents in trichrome staining technique for intestinal protozoa.
BDH xylene substitute, a terpene-based product, and its mixture with anhydrous alcohol were found to be excellent replacements for xylene and carbol-xylene, respectively, in the trichrome staining technique applied to sodium acetate-acetic acid-Formalin-fixed fecal smears for detection of intestinal protozoa.
Provincial Laboratory of Public Health for Southern Alberta, Calgary, Canada.
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Gubash
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Milburn
D000818:Animals; D000431:Ethanol; D056890:Eukaryota / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D007422:Intestines / Q000469:parasitology*; D013194:Staining and Labeling; D013729:Terpenes
D013729:Terpenes; D000431:Ethanol