Immunohistochemical analysis of p53 and HER-2/neu proteins in human tumors.
We examined samples of tumors of human breast, ovary, and colon of various degrees of malignancy for the expression of p53 protein, using a panel of anti-p53 antibodies and peroxidase immunohistochemistry. Of 66 tumor cases (24 cases of ovarian carcinoma, 23 cases of colon adenocarcinoma, and 19 cases of breast carcinoma), 36 (53%) showed high levels of expression of p53 using a human-specific antibody, and 16 (24%) showed high expression of a mutant form of p53. In the mutant p53-positive breast tumor samples, six (86%) were positive for HER-2/neu reactivity, compared with colon (0/4) and ovarian tumors (1/5). The pattern of p53 intracellular localization and tissue distribution, and the relationship between the expression of mutant p53 and cell differentiation, were also examined; poorly differentiated cells showed either overexpression of p53 or higher levels of mutant p53 in comparison with more normal cells.
Ultrastructural Cytochemistry Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
K
K
Chang
I
I
Ding
F G
FG
Kern
M C
MC
Willingham
D001943:Breast Neoplasms / Q000737:chemistry; D003110:Colonic Neoplasms / Q000737:chemistry; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D009154:Mutation; D009369:Neoplasms / Q000737:chemistry*; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D010051:Ovarian Neoplasms / Q000737:chemistry; D018719:Erb-b2 Receptor Tyrosine Kinases; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D009856:Oncogene Proteins, Viral; D018719:Erb-b2 Receptor Tyrosine Kinases; D016159:Tumor Suppressor Protein p53