Infection of monocytes/macrophages by HIV in vitro.
Because of the very important role of the mononuclear phagocyte system in the immunopathogenesis of HIV infection, a culture system for in vitro studies of infection of monocytes/macrophages with HIV was developed. A method is described for the infection of human monocytes/macrophages cultured on hydrophobic membranes (Teflon) with different strains of HIV. The HIV isolates can be characterized according to their replication potential on monocytes/macrophages cultures. The biological properties of some HIV1 and HIV2 isolates are compared in lymphocyte and monocyte/macrophage cultures.
Georg-Speyer-Haus, Chemotherapeutisches Forschungsinstitut, Frankfurt/M, FRG.
H
H
von Briesen
R
R
Andreesen
R
R
Esser
W
W
Brugger
C
C
Meichsner
K
K
Becker
H
H
Rübsamen-Waigmann
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000473:pathology*; D015497:HIV-1 / Q000254:growth & development*; D015498:HIV-2 / Q000254:growth & development; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D008567:Membranes, Artificial; D009000:Monocytes / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D011138:Polytetrafluoroethylene; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014776:Virus Cultivation
D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene; D012194:RNA-Directed DNA Polymerase