Reduced somatostatin-like immunoreactivity in the brain of LEC rats with hepatic encephalopathy.
Somatostatin-14-like immunoreactivity (S14LI) and somatostatin-28(1-12)-like immunoreactivity (S28(1-12)LI) in the brain of LEC (Long Evans Cinnamon) rats with hepatic encephalopathy were measured. Significant reduction of both S14LI and S28(1-12)LI was observed in the hypothalamus, medulla oblongata, striatum and spinal cord. Both of the immunoreactivities in the hypothalamus of these rats were approx. 50% of those in LEC rats without hepatic encephalopathy. The amounts of reduction of S14LI significantly correlated with those of reduction of S28(1-12)LI. No significant difference in gel chromatographic profiles of S14LI and S28(1-12)LI was observed between LEC rats with and without hepatic encephalopathy. These results suggest that the reduction of somatostatin-like immunoreactivity in LEC rats with hepatic encephalopathy may be caused by a decrease in production of prosomatostatin rather than altered degradation.
First Department of Biochemistry, School of Medicine, Fukuoka University, Japan.
K
K
Tateishi
Y
Y
Miura
O
O
Moriai
K
K
Suzuki
N
N
Takeichi
H
H
Kobayashi
Y
Y
Matsuoka
S
S
Sato
D000818:Animals; D001774:Blood Chemical Analysis; D002490:Central Nervous System / Q000737:chemistry* / Q000276:immunology; D003342:Corpus Striatum / Q000737:chemistry / Q000276:immunology; D004195:Disease Models, Animal; D006501:Hepatic Encephalopathy / Q000209:etiology*; D007031:Hypothalamus / Q000737:chemistry / Q000276:immunology; D008526:Medulla Oblongata / Q000737:chemistry / Q000276:immunology; D010446:Peptide Fragments / Q000032:analysis* / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000032:analysis* / Q000276:immunology; D053561:Somatostatin-28; D013116:Spinal Cord / Q000737:chemistry / Q000276:immunology
D010446:Peptide Fragments; D013004:Somatostatin; D053561:Somatostatin-28; C037197:somatostatin 28-(1-12)