Expression of the mouse macrophage cystine transporter in Xenopus laevis oocytes.
System xc- mediates transport of cystine and glutamate across the mammalian plasma membrane in a Na(+)-independent manner. This transport activity can be induced in mouse peritoneal macrophages during culture by diethylmaleate, a sulfhydryl-reactive agent. We injected mRNA from such macrophages into Xenopus oocytes and demonstrated the expression of System xc-, i.e., a Na(+)-independent, glutamate-inhibitable cysteine transport system. The expressed cystine transport activity depended on the assay temperature, in that cystine uptake measured at 37 degrees C was severalfold higher than that measured at 20 degrees C. Injection of size-fractionated mRNA indicated that the System xc- transporter of the mouse macrophage is encoded by mRNA of 1.5 to 2.9 kb.
Institute of Basic Medical Sciences, University of Tsukuba, Japan.
T
T
Ishii
K
K
Nakayama
H
H
Sato
K
K
Miura
M
M
Yamada
K
K
Yamada
Y
Y
Sugita
S
S
Bannai
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003553:Cystine / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D005971:Glutamates / Q000378:metabolism / Q000494:pharmacology; D018698:Glutamic Acid; D006863:Hydrogen-Ion Concentration; D008264:Macrophages / Q000737:chemistry*; D008298:Maleates / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D014162:Transfection; D014982:Xenopus laevis
D002352:Carrier Proteins; D005971:Glutamates; D008298:Maleates; D012333:RNA, Messenger; D018698:Glutamic Acid; D003553:Cystine; C014476:diethyl maleate