Molecular cloning of a human immunoglobulin heavy chain variable (VH) region with anti-myelin-associated glycoprotein activity.
A cDNA clone that encodes the heavy chain variable region (VH) of an IgM M-protein with anti-myelin-associated glycoprotein (MAG) activity secreted by chronic lymphocytic leukemia cells (B-C11) from a patient with peripheral neuropathy was cloned and sequenced. The JH region was identical to the germline JH4 sequence except for deletion of a thymidine residue at the site of D-JH recombination, and the D region showed greatest homology to DM2. Sequence analysis of the VH region revealed greatest homology to VH26, a member of the VH3 gene family, but homology was only 83.7% over 326 bases, suggesting that it was derived from as yet an unidentified member of the VH3 gene family.
Desai
R
R
Department of Neurology, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
Spatz
L
L
Matsuda
T
T
Ilyas
A A
AA
Berman
J E
JE
Alt
F W
FW
Kabat
E A
EA
Latov
N
N
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D006825:Hybridomas / Q000032:analysis; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000276:immunology*; D018992:Myelin-Associated Glycoprotein; D009693:Nucleic Acid Hybridization; D012313:RNA
D000906:Antibodies; D007143:Immunoglobulin Heavy Chains; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D012313:RNA; D004247:DNA