Interferon induced inhibition of queuine uptake in cultured human fibroblasts.
Interferon inhibits uptake of the radiolabeled queuine analog, rQT3, into cultured human fibroblasts. Simultaneous exposure to 10 nM phorbol-12,13-didecanoate (PDD) potentiates interferon-induced inhibition of rQT3 into cultured fibroblasts. All three major classes of human interferon tested affected uptake similarly, with fibroblast derived beta-interferon being more effective in dose response than gamma or alpha interferons. This suggests that endogenous production of interferon by cultured cells, such as that observed during a low grade viral infection, inhibits queuine uptake and may subsequently lead to a decreased level of queuine modified transfer RNA. Queuine-hypomodified transfer RNA has been implicated in growth control, differentiation and neoplastic transformation.
Elliott
M S
MS
Old Dominion University, Department of Chemistry and Biochemistry, Norfolk, Virginia 23529.
Crane
D L
DL
D001692:Biological Transport / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology*; D010703:Phorbol Esters / Q000494:pharmacology; D011070:Poly I-C / Q000494:pharmacology; D011994:Recombinant Proteins
D007370:Interferon Type I; D010703:Phorbol Esters; D011994:Recombinant Proteins; C008876:phorbol-12,13-didecanoate; D006147:Guanine; C019549:queuine; D007371:Interferon-gamma; D007372:Interferons; D011070:Poly I-C