Protein profile typing--a new method of typing Morganella morganii strains.
A new, simple and stable method for typing Morganella morganii strains is described. The 150 strains examined, principally from faeces, contained haemolytic and non-haemolytic representatives of diverse O serogroup, bacteriocin type and biotype. Among the biotypes were some trehalose-fermenting, tetracycline-resistant strains and some non-motile, tetracycline-sensitive, glycerol fermenters. After analysis of cell lysates by sodium dodecyl sulphate-discontinuous polyacrylamide gel electrophoresis, strains could be differentiated into 21 types on the basis of outer membrane proteins (OMP) of 35-40 Kda. The OMP profile was not altered by culture on various common media and was unrelated to either O antigen or morganocin p-type. The finest strain recognition in M. morganii can be achieved by application of all three distinct typing methods.
Department of Medical Microbiology, Dundee University Medical School, Ninewells Hospital.
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D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques; D001430:Bacteriocins; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000032:analysis / Q000145:classification* / Q000276:immunology / Q000378:metabolism; D006460:Hemolysin Proteins / Q000096:biosynthesis; D006461:Hemolysis; D006801:Humans; D019081:O Antigens; D012703:Serotyping
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001430:Bacteriocins; D006460:Hemolysin Proteins; D019081:O Antigens; C014687:morganocin 174