Association of a chicken repetitive element with the endogenous virus-21 slow-feathering locus.
The DNA sequences of an endogenous virus ev21-cell junction fragment (JFIL-1), and the pristine ev21 unoccupied region were analyzed. Comparisons of 3' proviral sequences of JFIL-1 with comparable regions of ev2, the prototype Rous-associated provirus (RAV-0), revealed minor single-base substitutions in the transmembrane domain of proviral envelope glycoprotein and the long terminal repeat of ev21. In the cell component of the JFIL-1, insertion of a 9-bp direct repeat and deletion of a guanine deoxynucleotide abolished HaeIII and BalI recognition sites that were present in the pristine region. Upstream from the insertion site, a large region was 71 and 75% homologous with dispersed chicken repetitive (CR1) elements associated with vitellogenin and very low density apolipoprotein II genes, respectively. Southern blot hybridizations and comparisons of CR1 motifs such as polypurine tracts, a tandem imperfect octamer, and 6-bp duplications indicated that this sex-linked, CR1 element (CR1ev21) may be the longest member of this family reported thus far.
U.S. Department of Agriculture, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
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I
Levin
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EJ
Smith
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000382:microbiology; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D005241:Feathers / Q000254:growth & development*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012150:Polymorphism, Restriction Fragment Length; D011533:Proviruses / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral; D004247:DNA