Primary culture of neurons derived from the adult mammalian brain.
A method is described for the isolation and culture of neurons in the adult mammalian brain. The cell could be maintained in primary culture for more than several weeks. Whereas the neurons freshly dissociated from the adult brain did not respond to any of the neurotransmitter substances applied, the neurons regained the ability to respond to a variety of neurotransmitters when cultured. The cultured neurons of the adult mammalian brain may be an excellent model for physiological as well as pharmacological investigations of the central nervous system.
Department of Pharmacology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
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Ogata
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Tatebayashi
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D046508:Culture Techniques / Q000379:methods; D004298:Dopamine / Q000494:pharmacology; D005071:Evoked Potentials / Q000187:drug effects; D005260:Female; D006168:Guinea Pigs; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009928:Organ Specificity; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D018377:Neurotransmitter Agents; D005680:gamma-Aminobutyric Acid; D004298:Dopamine