Cimaterol and clenbuterol residue analysis by HPLC-HPTLC in liver.
A method has been developed for the analysis of cimaterol and clenbuterol residues in liver, with detection limits of 0.25 micrograms/kg and 0.5 micrograms/kg, respectively. The recovery varied from 55% to 60%. After extraction, a clean-up procedure with Baker-spe C-18 columns was performed. The two chemical compounds of interest were eluted with methanol. Cimaterol and clenbuterol were quantitatively determined by high-performance liquid chromatography (HPLC) using an RP-Select B (5 microns) column and a post-column reaction procedure. The positive results were confirmed by high-performance thin-layer chromatography (HPTLC) as this technique reaches the same level of sensitivity as the HPLC method.
Ministère de la Santé Publique et de l'Environnement, Brussels, Belgium.
J M
JM
Degroodt
B
B
Wyhowski de Bukanski
J
J
De Groof
H
H
Beernaert
D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D002976:Clenbuterol / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D004983:Ethanolamines / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D011237:Predictive Value of Tests
D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; D002976:Clenbuterol; C047411:cimaterol