Studies on the non-activated and activated forms of the estrogen receptor.
The activation of the steroid receptor is a necessary process for the biological role of the receptor. Many factors are involved in this mechanism; in addition to time, temperature, and salt concentration, RNA and RNAase can also affect the transformation of the non-activated to the activated form of the receptor. Using as a model the estrogen receptor of fetal uterus of guinea-pig, the studies of the interaction with three different monoclonal antibodies (D547, H222 and H226) reveal structural transformation during the process of the receptor activation. These conformational transformations suggest that a change in the exposure of the functional domains of the estrogen receptor occurs during activation.
C.N.R.S. Steroid Hormone Research Unit Foundation for Hormone Research, Paris, France.
J R
JR
Pasqualini
N
N
Giambiagi
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D000939:Epitopes / Q000032:analysis; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D011487:Protein Conformation; D012313:RNA / Q000494:pharmacology; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D012260:Ribonucleases / Q000494:pharmacology; D014599:Uterus / Q000032:analysis / Q000196:embryology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011960:Receptors, Estrogen; D004958:Estradiol; D012313:RNA; D004247:DNA; D012260:Ribonucleases