Testicular steroidogenesis in the testicular feminized (Tfm) mouse: loss of 17 alpha-hydroxylase activity.
Testicular feminized (Tfm) mice are totally insensitive to androgen and may be used to study the role of the androgen receptor in normal development and function. We have examined testicular and Leydig cell steroidogenesis in Tfm mice. Serum bioactive LH was high in Tfm mice but serum testosterone was low and this was associated with a severe reduction in testicular testosterone production in vitro. Examination of [3H]pregnenolone metabolism by testes of Tfm mice indicated that progesterone, rather than testosterone, was the major steroid produced. Leydig cells were isolated from normal and Tfm mice and from normal mice in which testicular descent was surgically prevented before puberty. As in whole testes, androgen production in response to human chorionic gonadotrophin was severely reduced in Leydig cells from testes of Tfm mice compared with normal or cryptorchid groups. In contrast, progesterone production by Leydig cells from testes of Tfm mice was markedly increased in comparison with other groups. Total steroid production (progesterone plus androstenedione plus testosterone), however, was only 24% of normal in Leydig cells from Tfm mice. The pattern of steroid production by Leydig cells from cryptorchid testes was similar to control, although total steroid production was reduced to about 50% (this was significantly higher than the Tfm group, P less than 0.05). The high progesterone/androgen ratio in testes from Tfm mice suggested that 17 alpha-hydroxylase was depleted in these animals. To confirm this, activity of the four major steroidogenic enzymes associated with the smooth endoplasmic reticulum was measured.(ABSTRACT TRUNCATED AT 250 WORDS)
Murphy
L
L
Department of Veterinary Basic Sciences, Royal Veterinary College, London.
O'Shaughnessy
P J
PJ
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D013734:Androgen-Insensitivity Syndrome / Q000097:blood / Q000201:enzymology*; D000728:Androgens / Q000096:biosynthesis*; D000735:Androstenedione / Q000096:biosynthesis; D000818:Animals; D002478:Cells, Cultured; D003456:Cryptorchidism / Q000201:enzymology; D007985:Leydig Cells / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D011374:Progesterone / Q000096:biosynthesis; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism*; D013737:Testis / Q000201:enzymology*; D013739:Testosterone / Q000096:biosynthesis / Q000097:blood
D000728:Androgens; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D007986:Luteinizing Hormone; D015067:17-Hydroxysteroid Dehydrogenases; D013254:Steroid 17-alpha-Hydroxylase