Structure of human myeloma IgG3 Kuc.
An electron microscopy study of human myeloma IgG3 Kuc has shown that the hinge region in an intact molecule is in a compact state. The subunits are not fixed rigidly and are very mobile. These data are supported by results of ultracentrifugation and microcalorimetry. Non-extremal denaturating effects (pH 4.0, 20 degrees C or pH 7.8, 65 degrees C) lead to 'unfolding' of the hinge region which has a rod-like shape in electron micrographs.
Ryazantsev
S
S
Institute of Protein Research, Academy of Sciences, Pushchino, USSR.
Tishchenko
V
V
Vasiliev
V
V
Zav'yalov
V
V
Abramov
V
V
D005612:Freeze Drying; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000648:ultrastructure*; D007135:Immunoglobulin Variable Region / Q000502:physiology; D008854:Microscopy, Electron; D009101:Multiple Myeloma / Q000276:immunology*; D011487:Protein Conformation; D011489:Protein Denaturation; D013194:Staining and Labeling; D013696:Temperature; D014461:Ultracentrifugation
D007074:Immunoglobulin G; D007135:Immunoglobulin Variable Region