Dynamics of gene expression for a hippocampal glycoprotein elevated in Alzheimer's disease and in response to experimental lesions in rat.
A hippocampal poly(A) RNA, pADHC-9, was cloned by differential screening of a human hippocampal cDNA library. By RNA blot analysis, pADHC-9 was elevated 2-fold in Alzheimer's disease hippocampus. In situ analyses identified pADHC-9 expression in pyramidal and non-pyramidal cells of the hippocampus and entorhinal cortex. Nucleotide sequence analysis identified pADHC-9 as a potential human homolog of rat sulfated glycoprotein 2 (SGP-2). SGP-2 expression increased in rat hippocampus following experimental lesions that mimic intrinsic neuronal loss and/or deafferentation. The function of pADHC-9 in brain has not been defined, but in serum, a similar protein inhibits complement-dependent cytolysis. Increased expression of pADHC-9 in Alzheimer's disease hippocampus may be a compensatory response mounted to retard a complement-driven neurodegenerative cascade.
Andrus Gerontology Center, University of Southern California, Los Angeles 90089.
P C
PC
May
M
M
Lampert-Etchells
S A
SA
Johnson
J
J
Poirier
J N
JN
Masters
C E
CE
Finch
D000544:Alzheimer Disease / Q000378:metabolism*; D001345:Autoradiography; D003001:Cloning, Molecular; D051152:Clusterin; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D003714:Denervation; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics*; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D008297:Male; D018832:Molecular Chaperones; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics*; D014158:Transcription, Genetic
C495242:CLU protein, human; D051152:Clusterin; D015342:DNA Probes; D006023:Glycoproteins; D018832:Molecular Chaperones; D012313:RNA; D004247:DNA