Northern blot hybridization analysis of polyoma virus-specific RNA synthesized under the block of virus replication by 5-bromo-2'-deoxyuridine.
Polyoma (Py) virus-specific RNA, synthesized at reduced level in infected cells in the presence of antiviral compound 5-bromo-2'-deoxyuridine (BrdUrd) was characterized in more detail by Northern blot hybridization analysis. The results obtained with total, cytoplasmic and poly(A)RNA, isolated from mouse embryo cell cultures 42 hrs p.i. indicate that BrdUrd (6.34 micrograms/ml) lowers the level of typical classes of major virus DNA transcripts to a similar extent and that no new, atypical transcription products are formed.
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czecho-Slovakia.
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J
Tarábek
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J
Zemla
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I
Bacík
D015152:Blotting, Northern; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D011061:Poly A / Q000096:biosynthesis*; D011120:Polyomavirus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects*
D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D001973:Bromodeoxyuridine