Stable [57Co]-bleomycin complex with a very high specific radioactivity for use at very low concentrations.
A method for the preparation of 57Co-labelled bleomycin (BLM) possessing very high specific radioactivity and suitable for use at the nanomolar concentration range is described, and validated using a biological assay. Chelation of BLM with Co(II) results in a very stable complex. However, association does not occur below the micromolar concentration range. A nanomolar [57Co]BLM solution with maximal specific radioactivity can be easily prepared, without handling unreasonable amounts of radioactivity, provided that: equimolar solutions of BLM and [57Co]Cl2 are first mixed at the micromolar concentration range and that the mixture is then diluted a thousand times to reach the nanomolar concentration range.
U 140 INSERM, URA 147 CNRS, Institut Gustave Roussy, Villejuif, France.
B
B
Poddevin
J
J
Belehradek
L M
LM
Mir
D000818:Animals; D001761:Bleomycin / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology / Q000031:analogs & derivatives; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002850:Chromatography, Gel; D002999:Clone Cells; D003035:Cobalt; D003037:Cobalt Radioisotopes; D004558:Electric Stimulation; D007700:Kinetics
D003037:Cobalt Radioisotopes; C030187:cobalt bleomycin; D001761:Bleomycin; D003035:Cobalt; C018021:cobaltous chloride