Genetic stability of the D1Z2 region: implications for DNA genotyping and paternity testing.
The aim of the present study was to examine a single locus variable number tandem repeat for the purpose of DNA genotyping ("fingerprinting"). DNAs of 175 individuals from five ethnic groups (Black, Chinese, Japanese, Caucasian, and Melanesian) were analyzed. Restriction fragment length polymorphic analysis of random individuals revealed individual specific DNA patterns in all but one group. Among 20 Melanesian inhabitants of the Vanuatu islands in the southwest Pacific, three individuals were found to share a common pattern. This island population represents a "genetic isolate" and illustrates the importance of carrying out population studies on individual ethnic groups of interest. The complexity and the genetic stability of the D1Z2 region as revealed by the probe hMF1 make it an excellent candidate for DNA genotyping in paternity testing as 101 Caucasian individuals each had unique patterns for PstI and SinI digests.
Department of Medical Biochemistry, University of Calgary, Alta., Canada.
K M
KM
Tynan
M I
MI
Parslow
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DI
Hoar
D001483:Base Sequence; D015139:Blotting, Southern; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010334:Paternity; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D044465:White People / Q000235:genetics