Autoradiographic visualisation of [3H]DTG binding to sigma receptors, [3H]TCP binding sites, and L-[3H]glutamate binding to NMDA receptors in human cerebellum.
The autoradiographic distributions of [3H]1,3-di-ortho-tolyguanidine ([3H]DTG), [3H]1-[1-(2-thienyl) cyclohexyl] piperidine ([3H]TCP) and L-[3H]glutamate were studied in the human cerebellum. [3H]DTG is a selective label for the sigma receptor, while L-[3H]glutamate binding was carried out under conditions selective for the N-methyl-D-aspartate (NMDA) receptor. [3H]TCP binding sites and sigma receptors showed marked enrichment in the Purkinje cell layer, while L-[3H]glutamate-labelled NMDA receptors showed virtually no binding in the Purkinje cell layer. The results confirm the existence of [3H]TCP binding sites which are not linked to NMDA receptors in the human cerebellum, having a distribution which is more similar to that of the haloperidol-sensitive sigma receptor.
MRC Unit, Radcliffe Infirmary, Oxford, U.K.
K L
KL
Jansen
M
M
Dragunow
R L
RL
Faull
R A
RA
Leslie
D001345:Autoradiography / Q000379:methods; D005260:Female; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006146:Guanidines / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010622:Phencyclidine / Q000031:analogs & derivatives* / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D011957:Receptors, Opioid / Q000378:metabolism*; D017478:Receptors, Phencyclidine; D017480:Receptors, sigma; D014316:Tritium
D005971:Glutamates; D006146:Guanidines; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D011957:Receptors, Opioid; D017478:Receptors, Phencyclidine; D017480:Receptors, sigma; D014316:Tritium; D018698:Glutamic Acid; C012058:tenocyclidine; D010622:Phencyclidine; C050232:1,3-ditolylguanidine