In vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA.
The skin cells of newborn mice were stably transformed in vivo with the aid of electroporation. The plasmid DNA was introduced subcutaneously followed by high-voltage pulses applied to the skin pleat. NEO-resistant colonies were found in primary cell cultures obtained from the treated skin. The experiments show that in vivo electroporation can be used for the introduction of plasmid DNA into skin cells of mouse.
Institute of Molecular Biology, U.S.S.R. Academy of Sciences, Moscow.
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AV
Titomirov
S
S
Sukharev
E
E
Kistanova
D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D004247:DNA / Q000235:genetics*; D004560:Electricity; D005347:Fibroblasts / Q000166:cytology; D051379:Mice; D008808:Mice, Inbred CBA; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012313:RNA / Q000032:analysis; D012867:Skin / Q000166:cytology*; D014162:Transfection
D012313:RNA; D004247:DNA