Keratinocyte-expression of interleukin-6 but not of tumour necrosis factor-alpha is increased in the allergic and the irritant patch test reaction.
The cytokine expression on epidermal cells in the allergic patch test reaction (APR) and irritant patch test reaction (IPR) was studied using antibodies against rIL-6, rTNF alpha, rIL-1 alpha and rIL-1 beta in a histochemical biotin-avidin technique. Nickel sulphate was used for APR in 5 nickel allergic patients and sodium lauryl sulphate for IPR in 5 healthy individuals. The individuals served as their own control. Enhanced keratinocyte expression of IL-6 was observed in APR and IPR, whereas staining for TNF alpha remained unaltered compared with non-tested and petrolatum-tested skin. Staining for IL-1 alpha and IL-1 beta proved negative in all specimens. Double-staining experiments demonstrated that epidermal and dermal OKT-6 (CD1) positive Langerhans cells (LC) remained negative for all cytokines. These results demonstrate that enhancement of keratinocyte-bound IL-6 does not induce TNF alpha, IL-1 alpha/beta or IL-6 expression by LC during APR or IPR, and that enhanced keratinocyte expression of IL-6 fails to distinguish between these two reactions.
Department of Dermatology, University of California, San Francisco.
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Oxholm
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Oxholm
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Avnstorp
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Bendtzen
D000328:Adult; D003877:Dermatitis, Contact / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism*; D007509:Irritants; D015603:Keratinocytes / Q000378:metabolism*; D007801:Langerhans Cells / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009532:Nickel / Q000009:adverse effects; D010328:Patch Tests; D012867:Skin / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007375:Interleukin-1; D015850:Interleukin-6; D007509:Irritants; D014409:Tumor Necrosis Factor-alpha; D009532:Nickel