[Cytokeratin expression in squamous-cell metaplasia of the cervix uteri: an immunohistochemical study using monoclonal antibodies].
Immunohistochemical study of the exo- and endocervix in squamous metaplasia is performed using EE21-06d monoclonal antibodies revealing simultaneously five cytokeratinous polypeptides characteristic of squamous epithelium, and f-12-19h revealing simultaneously wide spectrum of cytokeratinous polypeptides characteristic of the epithelium various types. PAP method and the reaction of immunofluorescence were used. Monoclonal antibodies 12-19d label cells of all layers of normal exocervix, endocervix and metaplastic squamous epithelium, both immature and differentiated. Monoclonal antibodies EE21-06d react in the exocervix with suprabasal and higher located cells and in the endocervix with reserve cells only. Positive reaction with EE21-06d in the metaplastic foci increases in proportion with the squamous cell differentiation, thus this antibody being the marker of reserve cells and the degree of the squamous metaplasia maturity.
Raĭkhlin
N T
NT
Dobrynin
V A
VA
Petrov
S V
SV
Serre
G
G
D000328:Adult; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001706:Biopsy; D002584:Cervix Uteri / Q000378:metabolism* / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007633:Keratins / Q000276:immunology / Q000378:metabolism*; D008679:Metaplasia / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D007633:Keratins
Ekspressiia tsitokeratinov pri ploskokletochnoĭ metaplazii sheĭki matki: immunogistokhimicheskoe issledovanie s pomoshch'iu monoklonal'nykh antitel.