Gene disruption in mammals.
In the past year, the first phenotypes have been reported for mutations targeted to developmentally relevant genes by homologous recombination in embryonic stem cells. The results indicate that the genetic circuitry of mammalian development is complex and will require more sophisticated analysis than simple gene disruption. Improvements in the technology of targeted mutagenesis may assist in such analysis.
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
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Rossant
D000818:Animals; D005314:Embryonic and Fetal Development / Q000235:genetics*; D005801:Genes, Homeobox; D005822:Genetic Vectors; D006133:Growth Substances / Q000235:genetics / Q000502:physiology; D008322:Mammals / Q000196:embryology / Q000235:genetics*; D009024:Morphogenesis / Q000235:genetics; D016296:Mutagenesis; D016254:Mutagenesis, Insertional; D010641:Phenotype; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
D006133:Growth Substances; D014157:Transcription Factors