Drosophila retrotransposon promoter includes an essential sequence at the initiation site and requires a downstream sequence for full activity.
We describe a 98-base-pair region (-38 to +60) in the long terminal repeat of the Drosophila gypsy retrotransposon that is sufficient for accurate normal-level transcription. We find that, unlike most RNA polymerase II (pol II) promoters, the gypsy promoter includes downstream sequences that are required for full activity. Also unlike most pol II promoters, the gypsy promoter, which lacks a TATA motif, was found to have an essential sequence at the transcription initiation site, mutation of which abolishes transcription. These three uncommon features of the gypsy promoter may be characteristic of a subset of pol II promoters, exemplified by certain retrotransposons and developmental genes of Drosophila and by Tdt, the mouse terminal deoxynucleotidyl-transferase (TdT) gene.
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
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Jarrell
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Meselson
D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012319:RNA Polymerase II / Q000235:genetics; D012333:RNA, Messenger; D014158:Transcription, Genetic; D014162:Transfection
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D012319:RNA Polymerase II