Characterization of chromosomes and localization of the rDNA locus in the aye-aye (Daubentonia madagascariensis).
The karyotype of a prosimian primate, the aye-aye (Daubentonia madagascariensis), is described. Results from a variety of staining methods (Q-, R-, G-, and C-banding, distamycin A/DAPI and methyl-green/DAPI) are reported. Sites of methylation were visualized using antibodies against 5-methylcytosine. Digestion of aye-aye fixed metaphase chromosomes with the restriction endonuclease HaeIII produced G-banding. No other restriction enzymes tested produced clear G- or C-banding patterns. Ag-staining of the nucleolar organizer regions (NORs) revealed the location of rDNA sites on the short arms of the smallest pairs of acrocentric chromosomes, 13p and 14p.
Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, MI 48201.
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Tagle
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Goodman
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Miller
D044503:5-Methylcytosine; D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics*; D007621:Karyotyping; D008297:Male; D008745:Methylation; D012834:Silver; D013194:Staining and Labeling; D011443:Strepsirhini / Q000235:genetics*
D004275:DNA, Ribosomal; D012834:Silver; D044503:5-Methylcytosine; D003596:Cytosine; D004262:DNA Restriction Enzymes