Cellular and molecular characteristics of transformed T cells from an antigen-specific T-cell line.
An antigen-specific T-cell line which transforms into T-lymphoma cells in vitro but apparently not in vivo is described. Membrane markers, tumorigenicity and T-cell receptor (TcR) V alpha and V beta-gene usage of the in vitro transformed T-cell line were analysed to investigate whether the transformation event was poly-, oligo-, or monoclonal. The results indicate that the T lymphoma has no chromosome abnormalities, contains no tumour-inducing virus, can induce clone-specific immunity, and is oligoclonal with respect to TcR V alpha and V beta expression. The nature of the transformation event and clinical application of vaccination against T lymphomas is discussed. In addition, the expressed TcR V alpha and V beta repertoire of Con A T blasts was apparently not affected by the Igh-l or the MHC haplotype, as investigated in Igh-l and MHC congeneic C57Bl mice.
Institute for Experimental Immunology, University of Copenhagen, Denmark.
A M
AM
Wegener
B
B
Holm
C
C
Geisler
B
B
Rubin
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D015152:Blotting, Northern; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D000939:Epitopes / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D010641:Phenotype; D012334:RNA, Neoplasm / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015342:DNA Probes; D004273:DNA, Neoplasm; D000939:Epitopes; D007135:Immunoglobulin Variable Region; D012334:RNA, Neoplasm; D011948:Receptors, Antigen, T-Cell