CD44 is the principal cell surface receptor for hyaluronate.
CD44 is a broadly distributed cell surface protein thought to mediate cell attachment to extracelular matrix components or specific cell surface ligands. We have created soluble CD44-immunoglobulin fusion proteins and characterized their reactivity with tissue sections and lymph node high endothelial cells in primary culture. The CD44 target on high endothelial cells is sensitive to enzymes that degrade hyaluronate, and binding of soluble CD44 is blocked by low concentrations of hyaluronate or high concentrations of chondroitin 4- and 6-sulfates. A mouse anti-hamster hyaluonate receptor antibody reacts with COS cells expressing hamster CD44 cDNA. In sections of all tissues examined, including lymph nodes and Peyer's patches, predigestion with hyaluronidase eliminated CD44 binding.
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
A
A
Aruffo
I
I
Stamenkovic
M
M
Melnick
C B
CB
Underhill
B
B
Seed
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000235:genetics*; D001483:Base Sequence; D001667:Binding, Competitive; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D004727:Endothelium / Q000378:metabolism; D006025:Glycosaminoglycans / Q000494:pharmacology; D018960:Hyaluronan Receptors; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules; D006025:Glycosaminoglycans; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; D015345:Oligonucleotide Probes; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D011993:Recombinant Fusion Proteins