[Simple in vitro test systems for determining the toxicity of environmental chemicals: microcultures of human lymphocytes and monoxenic ciliate cultures].
Two test methods were developed to assess (I) the cytotoxicity, (II) the environmental toxicity of chemicals. Test parameter for (I) is the growth inhibition of human T-lymphocytes stimulated by PHA (test period 6-7 days), for (II) the inhibition of cell multiplication of the ciliate Tetrahymena pyriformis grown monoxenically in a chemically defined medium which contained glucose and citrate as only carbon source (test period 13-14 days). The EC50 values (mol/l) of Pb, Cd, Hg, dichlorvos, atrazine and lindane were for (I): greater than or equal to 10(-2,5), 10(-5,4), 10(-5,6), 10(-5,0), 10(-4,75) and 10(-3,95) respectively, for (II): 10(-6,72), less than or equal to 10(-6,66), 10(-7,84), 10(-3,61), 10(-3,93) and 10(-4,75) respectively. The advantages of the described bioassays are simple handling and high sensitivity.
Technischen Universität München, Abteilung für Umwelthygiene und Impfwesen.
H C
HC
Huber
W
W
Huber
U
U
Ritter
D000818:Animals; D001280:Atrazine / Q000633:toxicity; D001681:Biological Assay; D002104:Cadmium / Q000633:toxicity; D002478:Cells, Cultured; D003470:Culture Media; D004006:Dichlorvos / Q000633:toxicity; D001556:Hexachlorocyclohexane / Q000633:toxicity; D006801:Humans; D007854:Lead / Q000633:toxicity; D008213:Lymphocyte Activation / Q000187:drug effects*; D008628:Mercury / Q000633:toxicity; D008670:Metals / Q000633:toxicity*; D010575:Pesticides / Q000633:toxicity*; D013601:T-Lymphocytes / Q000187:drug effects*; D013769:Tetrahymena pyriformis / Q000187:drug effects* / Q000254:growth & development
D003470:Culture Media; D008670:Metals; D010575:Pesticides; D002104:Cadmium; D007854:Lead; D001556:Hexachlorocyclohexane; D004006:Dichlorvos; D008628:Mercury; D001280:Atrazine
Einfache in vitro-Prüfsysteme zur Toxizitätsbestimmung von Umweltchemikalien: Mikrokulturen menschlicher Lymphozyten und monoxenische Ciliatenkulturen.