Endothelial expression of intercellular adhesion molecule (ICAM-1) is unaffected by marrow aplasia-inducing chemotherapy.
Sequential skin biopsies were obtained from five patients receiving chemotherapy for acute myelocytic leukemia. The first specimen was taken on the fourth day of initial chemotherapy and subsequent specimens were obtained at weekly intervals thereafter, until discharge. In all cases, clinically normal skin was selected as the biopsy site. A panel of monoclonal antibodies was applied to frozen tissue sections, including anti-intercellular adhesion molecule-1 (ICAM-1), anti-lymphocyte function-associated antigen-1 (LFA-1), and anti-HLA-DR. Bound antibody was detected using the avidin-biotin complex method. Endothelial cell expression of ICAM-1 and lymphocyte expression of LFA-1 were unaffected over the course of the ensuing, profound marrow aplasia. This finding suggests that this mechanism of lymphocyte trafficking in the skin remains operational despite the administration of antileukemic agents.
Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland.
T D
TD
Horn
B J
BJ
Kerker
J E
JE
Karp
P J
PJ
Burke
A F
AF
Hood
D000677:Amsacrine / Q000494:pharmacology; D015703:Antigens, CD / Q000032:analysis; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000032:analysis*; D003561:Cytarabine / Q000494:pharmacology; D003630:Daunorubicin / Q000494:pharmacology; D004727:Endothelium; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007951:Leukemia, Myeloid / Q000188:drug therapy; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D012867:Skin / Q000378:metabolism*
D015703:Antigens, CD; D000970:Antineoplastic Agents; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D000677:Amsacrine; D003561:Cytarabine; D018799:Intercellular Adhesion Molecule-1; D003630:Daunorubicin