Cloning from Streptomyces cellulosae of the gene encoding beta-lactamase, a blue-dextran binding protein.
A beta-lactamase gene was cloned from Streptomyces cellulosae as a 2.3-kb DNA fragment using Streptomyces lividans 1326 and PIJ385 as a host-vector system. During the course of cloning, a part of the chromosomal DNA fragment cloned together with a part of the vector plasmid were deleted, indicating instability of this contiguous DNA region. The enzyme from the clone showed similar properties with respect to binding of blue dextran and isoelectric point to the enzyme from S. cellulosae. The cloned gene hybridized not only to DNA of S. cellulosae, the source of DNA, but also to DNAs of several Streptomyces species, irrespective of their formation of beta-lactamase. These results suggest that this gene may have homology to genes other than the one for beta-lactamase.
Department of Biochemistry, Meiji College of Pharmacy, Tokyo, Japan.
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Urabe
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Toyama
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Ogawara
D015139:Blotting, Southern; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004396:Coloring Agents / Q000378:metabolism; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D003911:Dextrans / Q000378:metabolism*; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
D004396:Coloring Agents; D015342:DNA Probes; D004269:DNA, Bacterial; D003911:Dextrans; C017080:blue dextran; D001618:beta-Lactamases