An unusual case using DNA polymorphisms to determine parentage of human remains.
The 2-year-old daughter of two farm laborers was reported missing while the farm owner was harvesting corn. Unidentifiable tissues and body parts were subsequently found admixed with silage. Samples of blood collected from the parents of the missing child as well as portions of the tissue recovered from the silage were subjected to analysis of DNA polymorphisms with probes usually used to identify paternity. In addition, allele-specific oligonucleotides were used to detect DNA polymorphism at the DQ alpha locus following DNA amplification using the polymerase chain reaction. In this case, the DNA results established that the tissue recovered from the silage was of human origin and confirmed the probable parentage of the two farm laborers.
Department of Pathology, University of Vermont College of Medicine, Burlington 05405.
P
P
Mulhare
E
E
McQuillen
C
C
Collins
N
N
Heintz
P
P
Howard
D000059:Accidents; D000328:Adult; D000483:Alleles; D002675:Child, Preschool; D004247:DNA / Q000032:analysis*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D006683:HLA-DQ Antigens / Q000235:genetics; D006801:Humans; D008297:Male; D009035:Mothers; D015345:Oligonucleotide Probes; D010290:Parents; D010334:Paternity; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012820:Silage
D006683:HLA-DQ Antigens; D015345:Oligonucleotide Probes; D004247:DNA