Evaluation of an rRNA-derived oligonucleotide probe for culture confirmation of Neisseria gonorrhoeae.
The reliability of an rRNA-derived oligonucleotide probe for Neisseria gonorrhoeae was tested with 187 N. gonorrhoeae isolates, 81 Neisseria meningitidis isolates, and several strains of other bacterial species. The probe proved to be 100% specific and 100% sensitive. N. gonorrhoeae cells could also be reliably identified in contaminated cultures with the oligonucleotide probe. The 2.6-megadalton cryptic plasmid used as a probe for N. gonorrhoeae was shown to be less sensitive, detecting 179 of 181 N. gonorrhoeae isolates.
Innogenetics N.V., Antwerp, Belgium.
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R
Rossau
M
M
Duhamel
E
E
Van Dyck
P
P
Piot
H
H
Van Heuverswyn
D015342:DNA Probes; D005069:Evaluation Studies as Topic; D006069:Gonorrhea / Q000175:diagnosis; D006801:Humans; D015336:Molecular Probe Techniques; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000302:isolation & purification; D009345:Neisseria meningitidis / Q000235:genetics / Q000302:isolation & purification; D010957:Plasmids; D012329:RNA, Bacterial / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics; D013045:Species Specificity
D015342:DNA Probes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal