Comparative studies on tat mutants of three primate lentiviruses.
A frame-shift tat gene mutant of human immunodeficiency virus type 1 (HIV-1), which showed no detectable trans-activation potential, was constructed in vitro. Upon transfection, this clone directed the synthesis of virus mRNAs, gag proteins and virion-associated reverse transcriptase (RT) at a low level as was observed with the tat mutants of HIV-2 and simian immunodeficiency virus isolated from African green monkey (SIVAGM). Using these mutant viruses, trans-activation efficiency of viral gene expression by tat was compared among HIV-1, HIV-2, and SIVAGM. SIVAGM seemed to be less dependent on tat for RT production than HIV-1 and HIV-2.
Department of Viral Oncology, Kyoto University, Japan.
R
R
Shibata
H
H
Sakai
K
K
Ogawa
A
A
Ishimoto
A
A
Adachi
D001483:Base Sequence; D004279:DNA, Viral; D016368:Frameshift Mutation; D015967:Gene Expression Regulation, Viral; D015756:Genes, tat; D015497:HIV-1 / Q000235:genetics*; D015498:HIV-2 / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D011533:Proviruses / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis / Q000235:genetics; D015302:Simian Immunodeficiency Virus / Q000235:genetics; D015534:Trans-Activators; D014162:Transfection; D014407:Tumor Cells, Cultured
D004279:DNA, Viral; D015534:Trans-Activators; D012194:RNA-Directed DNA Polymerase