Theoretical conformational analysis of a family of alpha-helical immunocytokines.
According to the results of the theoretical conformation analysis and available experimental data, the known immunocytokines can be divided into two groups: alpha-helical (IFNs-alpha, beta, gamma, omega; IL-2, 3, 4, 5, 6, 7; G-, M-, GM-CSFs; cMGF, PDGF) and beta-pleated proteins (ILs-1 alpha, beta; TNFS-alpha, beta). IFNs-alpha, beta, gamma, omega, IL-6, G-CSF, cMGF were shown to form a family of alpha-helical globular proteins characterized by a statistically significant homology in amino acid sequences and by common features of the secondary structure formation. Comparison of the sequences of 72 IFNs-alpha, beta, omega reveals three clusters of conservative amino acid positions. Their participation in the formation of active sites of IFN-alpha, beta, omega is supposed.
Institute of Immunology, Moscow Region, Chekhov District, U.S.S.R.
V P
VP
Zav'yalov
A I
AI
Denesyuk
G A
GA
Zav'yalova
D000595:Amino Acid Sequence; D003115:Colony-Stimulating Factors / Q000737:chemistry*; D016207:Cytokines / Q000737:chemistry*; D006801:Humans; D007372:Interferons / Q000737:chemistry*; D007378:Interleukins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation
D003115:Colony-Stimulating Factors; D016207:Cytokines; D007378:Interleukins; D007372:Interferons