Growth factor stimulation of proliferating cell nuclear antigen (PCNA) in human breast epithelium in organ culture.
Normal human breast explants were maintained in serum-free culture for 7 days in the presence of either insulin hydrocortisone and cholera toxin (I/H/CT), epidermal growth factor (EGF) or transforming growth factor-alpha (TGF-alpha). Explants were labelled with [3H] thymidine, fixed in methacarn and processed for autoradiography. Parallel sections were immunolabelled with anti-PCNA antibody and analysed with a CAS 200 image analyser. Thymidine labelling index (TLI) and PCNA expression produced similar results with both indices increased in response to I/H/CT, EGF and TGF-alpha. In sections double labelled for PCNA and autoradiography the majority of labelled cells were positive for both markers.
Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, UK.
P
P
Monaghan
N P
NP
Perusinghe
R I
RI
Nicholson
R A
RA
McClelland
M J
MJ
O'Hare
D P
DP
Lane
H
H
Jayatilake
B A
BA
Gusterson
D001324:Autoantigens / Q000378:metabolism; D001940:Breast / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002772:Cholera Toxin / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D005260:Female; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D009687:Nuclear Proteins / Q000276:immunology* / Q000378:metabolism; D009924:Organ Culture Techniques; D018809:Proliferating Cell Nuclear Antigen; D013936:Thymidine / Q000378:metabolism; D016211:Transforming Growth Factor alpha / Q000494:pharmacology
D001324:Autoantigens; D006133:Growth Substances; D007328:Insulin; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor; D002772:Cholera Toxin; D013936:Thymidine; D006854:Hydrocortisone