Efflux-mediated multidrug resistance in Bacillus subtilis: similarities and dissimilarities with the mammalian system.
Bacillus subtilis cells selected for their resistance to rhodamine 6G demonstrated a multidrug-resistance (MDR) phenotype resembling that of mammalian MDR cells. Like MDR in mammalian cells, MDR in bacteria was mediated by the efflux of the drugs from the cells. The bacterial multidrug efflux system transported similar drugs and was sensitive to similar inhibitors as the mammalian multidrug transporter, P-glycoprotein. The gene coding for the bacterial multidrug transporter, like the P-glycoprotein gene in mammalian MDR cells, was amplified in the resistant bacteria. On the other hand, the bacterial multidrug transporter showed no sequence similarity to P-glycoprotein but exhibited an obvious homology to tetracycline efflux pumps and carbohydrate-ion symporters. These results show that the transport of structurally unrelated molecules can be mediated by members of different families of membrane transporters.
Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
A A
AA
Neyfakh
V E
VE
Bidnenko
L B
LB
Chen
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004351:Drug Resistance / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D004996:Ethidium / Q000378:metabolism; D008322:Mammals; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011487:Protein Conformation; D011815:R Factors; D015183:Restriction Mapping; D012235:Rhodamines / Q000494:pharmacology*; D012689:Sequence Homology, Nucleic Acid; D013753:Tetracycline Resistance / Q000235:genetics
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D008562:Membrane Glycoproteins; D012235:Rhodamines; C026188:rhodamine 6G; D004996:Ethidium