Influence of reserpine administration on neuropeptide Y immunoreactivity in the locus coeruleus and caudate-putamen nucleus of the rat brain.
The effects of treatment with reserpine (10 mg/kg, i.p.) a monoamine depleting agent, on neuropeptide Y immunoreactivity were studied immunohistochemically in neurons of two rat brain structures: locus coeruleus and caudate-putamen nucleus. It was found that reserpine after 24 h increased neuropeptide Y immunoreactivity level but no significant changes were observed 4 and 72 h or 5 days after the injection. The results indicate that despite the known co-existence of neuropeptide Y and noradrenaline in some neurons of the locus coeruleus no concomitant decrease in neuropeptide Y immunoreactivity level was found after reserpine when noradrenaline was depleted from nerve cell bodies and terminals. The increase in neuropeptide Y immunoreactivity observed 24 h after reserpine injection may suggest that the neuropeptide Y-containing neuronal systems of the locus coeruleus and caudate-putamen nucleus are controlled by monoaminergic afferents.
Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland.
M
M
Smiałowska
B
B
Legutko
D000818:Animals; D002421:Caudate Nucleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology; D007150:Immunohistochemistry; D008125:Locus Coeruleus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009478:Neuropeptide Y / Q000276:immunology / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D011699:Putamen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012110:Reserpine / Q000494:pharmacology*; D013194:Staining and Labeling
D009478:Neuropeptide Y; D012110:Reserpine; D003078:Colchicine; D009638:Norepinephrine