Structure of the complete human c-fgr proto-oncogene and identification of multiple transcriptional start sites.
Here we report the isolation of cosmid clones containing the whole of the human c-fgr proto-oncogene. We have used these to determine the organization and nucleotide sequence of the exons upstream of exon 4, which have not previously been analysed. We show that, like exons 4 to 12, exon 3 closely resembles its counterpart in the avian c-src and murine lck genes. In contrast, the organization of the upstream exons of c-fgr differs markedly. In particular, the 5' untranslated regions of the c-fgr gene is interrupted by an extra intron. We have also mapped multiple transcriptional start sites within the 5' flanking region of the c-fgr gene and present an analysis of the nucleotide sequences within this region which may be responsible for regulating c-fgr expression.
Department of Biochemistry, University College of Middlesex School of Medicine, London, UK.
M
M
Patel
S J
SJ
Leevers
P M
PM
Brickell
D001483:Base Sequence; D005091:Exons; D006801:Humans; D007438:Introns; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016392:Proto-Oncogene Proteins pp60(c-src); D011519:Proto-Oncogenes; D014158:Transcription, Genetic; D019061:src-Family Kinases
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D016392:Proto-Oncogene Proteins pp60(c-src); C053102:proto-oncogene proteins c-fgr; D019061:src-Family Kinases