Growth-factor independence of a new differentiated hepatitis B virus DNA-negative human hepatoma cell line.
The establishment of a new, differentiated, hepatitis B virus DNA-negative, human hepatoma cell line (named PLC/AN/2) is described. Neoplastic liver tissue was obtained during hepatectomy in an HBsAg-negative man. The established cell line is negative for alpha-fetoprotein and carcinoembryonic antigen; it has retained in vitro some of the differentiated functions of normal hepatocytes. Additionally, it presents a distinctive rearrangement (translocation) at the long arm of chromosome 4. The high degree of independence from serum growth factor requirements appears to be a major in vitro characteristic of PLC/AN/2 cells, making them a suitable model system for the more precise definition of the human hepatocellular carcinoma phenotype, including mechanisms of growth control.
Institute of Microbiology, University of Ancona, Italy.
P
P
Bagnarelli
G
G
Devescovi
A
A
Manzin
I
I
Bearzi
P
P
Bonazzi
G
G
Carloni
M
M
Clementi
D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D003470:Culture Media; D004279:DNA, Viral / Q000378:metabolism*; D006133:Growth Substances / Q000378:metabolism / Q000494:pharmacology; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D007633:Keratins / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D003470:Culture Media; D004279:DNA, Viral; D006133:Growth Substances; D007633:Keratins