Detection of a fast isoform of C-protein with an antiserum directed against the N-terminal portion of dystrophin.
An antiserum raised against the N-terminal actin-binding portion of dystrophin cross-reacted with a 130-kDa protein in fast skeletal muscle. The results of purification and two-dimensional gel electrophoresis and its immunological properties demonstrated that this protein is identical to a 130-kDa basic isoform of fast-C-protein. These results suggest that the actin-binding domain of dystrophin shares one or more antigenic determinants with those of C-protein.
Division of Neuromuscular Research, National Institute of Neuroscience, Tokyo, Japan.
M
M
Yamaguchi
S
S
Ishiura
H
H
Takano-Ohmuro
T
T
Tsukahara
K
K
Arahata
T
T
Obinata
T
T
Tamiya
T
T
Tsuchiya
H
H
Sugita
D000906:Antibodies; D002352:Carrier Proteins; D003429:Cross Reactions; D016189:Dystrophin; D000939:Epitopes / Q000032:analysis; D006801:Humans; D009124:Muscle Proteins / Q000032:analysis* / Q000276:immunology; D009132:Muscles / Q000032:analysis*; D000099163:Myosin Binding Protein C
D000906:Antibodies; D002352:Carrier Proteins; D016189:Dystrophin; D000939:Epitopes; D009124:Muscle Proteins; D000099163:Myosin Binding Protein C