Regulation of self-major histocompatibility complex reactive human T-cell clones.
The proliferative response of human T-lymphocyte clones, (TLC) specific for self-major histocompatibility complex (MHC) products either alone or associated with PPD epitopes are inhibited in vitro by dexamethasone (DEX) and by a non-specific inhibitory factor(s) (nsINH) produced by PPD-activated T-cells. The inhibiting effect has been investigated by preincubating autoreactive and PPD-specific TLC with nsINH or DEX. Results obtained indicate that T-lymphocytes are the target of these two immunoregulatory molecules. Moreover, the addition of exogenous recombinant interleukin 2 (rIL-2) substantially reverses the inhibition observed in both nsINH- or DEX-treated cultures.
Department of Cellular and Developmental Biology, I University of Rome, Italy.
M S
MS
Gilardini Montani
F
F
Del Gallo
M
M
Gobbi
G
G
Lombardi
E
E
Piccolella
O
O
Pugliese
V
V
Colizzi
D000942:Antigens, Bacterial / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D015536:Down-Regulation / Q000276:immunology; D000939:Epitopes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007111:Immunity, Cellular / Q000187:drug effects; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology; D008285:Major Histocompatibility Complex / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013696:Temperature
D000942:Antigens, Bacterial; D000939:Epitopes; D003907:Dexamethasone