Expression of raf family proto-oncogenes in normal mouse tissues.
We have determined RNA expression patterns of raf family proto-oncogenes in a variety of normal NFS/n mouse tissues, and several murine cell lines. Tissues were collected from male and female adults, and 16 day old fetuses. Raf-1 transcripts of 3.1 kb were found to be expressed in all tissues examined with highest levels in striated muscle, cerebellum and fetal brain. In contrast, A-raf showed wider variation in its range of expression, with the 2.6 kb transcripts being most abundant in epididymis and ovary. B-raf was found to have a very restricted expression pattern, with high levels in fetal brain and adult cerebrum. In addition to the 10 and 13 kb transcripts common to all B-raf expressing tissues, alternate sized B-raf RNAs were detected in testes, placenta and fetal membranes. We found no effect of gender on raf expression in non-sex related tissues, nor does tissue germline of origin correlate with levels of expression for any of the three genes.
Storm
S M
SM
Viral Pathology Section, National Cancer Institute, Frederick, Maryland 21701-1013.
Cleveland
J L
JL
Rapp
U R
UR
D000818:Animals; D015152:Blotting, Northern; D005260:Female; D005333:Fetus; D015870:Gene Expression; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf; D011519:Proto-Oncogenes; D012313:RNA / Q000235:genetics / Q000302:isolation & purification
D011518:Proto-Oncogene Proteins; D012313:RNA; D011505:Protein-Tyrosine Kinases; D019908:Proto-Oncogene Proteins c-raf