Monoclonal antibodies block murine IL-4 receptor function.
IL-4 is a cytokine which can induce B-lymphocyte proliferation, increase cell-surface Ia expression, and induce some activated B cells to differentiate and begin to secrete IgE. IL-4 binds specifically to a cell-surface receptor (IL-4R) on cells from a variety of lineages including T and B cells. In general both primary cells and in vitro cell lines express less than 5000 receptors per cell. Utilizing a subclone of the cytotoxic T cell line CTLL-2 expressing a high level of IL-4R, mAb against the murine IL-4R were prepared. Two mAb have been identified which have different properties. These antibodies, designated M1 and M2, recognize sequences specific to the murine IL-4R. Immunoprecipitation studies with M1 and M2 on CTLL-2 cells have identified the receptor as a Mr = 145,000 cell-surface protein. Similar results have been obtained with the recently isolated full length murine IL-4R cDNA expressed in COS-7 cells. In addition the antibodies are capable of inhibiting IL-4 binding. One antibody, M1, is also a potent inhibitor of IL-4-induced proliferation. These antibodies will be useful in dissecting a wide array of activities attributed to IL-4.
Immunex Corporation, Seattle, WA 98101.
M P
MP
Beckmann
K A
KA
Schooley
B
B
Gallis
T
T
Vanden Bos
D
D
Friend
A R
AR
Alpert
R
R
Raunio
K S
KS
Prickett
P E
PE
Baker
L S
LS
Park
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D002478:Cells, Cultured; D000939:Epitopes; D066298:In Vitro Techniques; D015847:Interleukin-4 / Q000502:physiology*; D008213:Lymphocyte Activation; D051379:Mice; D011233:Precipitin Tests; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen; D015847:Interleukin-4