Dynamic changes in 13C NMR spectra of intact hearts under conditions of varied metabolite enrichment.
Dynamic changes in 13C NMR signal from enriched glutamate pools within hearts have been examined under varied conditions of metabolite pool size and fractional enrichment. Relative signal intensities of 13C-enriched glutamate isotope isomers were similar within spectra from both intact hearts and corresponding in vitro samples. The parameters used to assess metabolic activity with 13C NMR proved independent of fractional enrichment and pool size. The data show the importance of acknowledging unlabeled, 13C NMR invisible metabolites.
Lewandowski
E D
ED
Section of Cardiology, Baylor College of Medicine, Houston, Texas 77030.
Hulbert
C
C
D000818:Animals; D002247:Carbon Isotopes; D005971:Glutamates; D018698:Glutamic Acid; D006321:Heart / Q000033:anatomy & histology*; D009682:Magnetic Resonance Spectroscopy; D009206:Myocardium / Q000378:metabolism; D010477:Perfusion; D011817:Rabbits
D002247:Carbon Isotopes; D005971:Glutamates; D018698:Glutamic Acid