Differences in the repertoire expressed by peritoneal and splenic Ly-1 (CD5)+ B cells.
Purified populations of B cells expressing the Ly-1 and/or Mac-1 surface Ag were isolated from normal unmanipulated mice by cell sorting. The number of lymphocytes in each population secreting antibodies reactive with DNA, bromelain-treated mouse RBC, phosphorylcholine and TNP-keyhole limpet hemocyanin was quantitated by ELISA spot assay. The proportion of B cells secreting Ig in vivo and the repertoire of antibodies they produced varied as a function of B cell phenotype and location. Among peritoneal lymphocytes, those that were Ly-1+ or Ly-1- Mac-1+ secreted Ig 10 times more frequently that Mac-1- Ly-1- B cells from the same location. In addition, the former populations expressed repertoires that were significantly skewed toward the production of antibodies reactive with bromelain-treated mouse RBC (p less than 0.001). In contrast, splenic B cells expressing the Ly-1 surface Ag did not differ significantly from splenic Ly-1- B cells in their expressed repertoire or frequency of Ig production. B cells isolated from the spleen and peritoneum tended to differ in antibody specificity from bone marrow and lymph node-derived lymphocytes. For example, B cells from the spleen secreted anti-DNA antibodies two to four times more frequently than B cells from other organs. These results demonstrate that phenotype and microenvironment influence the repertoire of antibodies expressed by B cells in vivo.
Klinman
D M
DM
Division of Virology, CBER, FDA, Bethesda, MD 20892.
Holmes
K L
KL
D000818:Animals; D000916:Antibody Diversity; D000917:Antibody Formation; D000943:Antigens, Differentiation / Q000032:analysis / Q000276:immunology; D000950:Antigens, Ly / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D018956:CD5 Antigens; D005434:Flow Cytometry; D016177:Macrophage-1 Antigen; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology; D016028:Receptors, Leukocyte-Adhesion / Q000032:analysis; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000943:Antigens, Differentiation; D000950:Antigens, Ly; D018956:CD5 Antigens; D016177:Macrophage-1 Antigen; D016028:Receptors, Leukocyte-Adhesion