Tetracycline and 13-cis-retinoic acid inhibit production and activity of granulocyte activating factor (GAF) from Propionibacterium acnes.
The aim of this study was to evaluate different treatment schedules on release or activity of a granulocyte activating factor (GAF) from Propionibacterium acnes. Incubation of P. acnes in physiological saline (30 min, 37 degrees C) resulted in release of a soluble factor that elicited considerable chemiluminescence response and chemotactic stimulus on human granulocytes. Pretreatment of the microorganisms with subinhibitory concentrations of tetracycline or incubation of granulocytes with 13-cis-retinoic acid significantly reduced the activating potency of GAF on these phagocytic cells. Since GAF was considered to be one of the stimuli for inflammation in acne vulgaris, administration of tetracycline and 13-cis-retinoic acid appears to be an adequate therapy.
Institute of Medical Microbiology and Hygiene, University of Cologne.
G
G
Pulverer
H L
HL
Ko
J
J
Beuth
W
W
Roszkowski
D002634:Chemotaxis, Leukocyte; D016179:Granulocyte Colony-Stimulating Factor / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D008163:Luminescent Measurements; D010587:Phagocytosis; D011425:Propionibacterium acnes / Q000187:drug effects* / Q000276:immunology; D013752:Tetracycline / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*
D016179:Granulocyte Colony-Stimulating Factor; D014212:Tretinoin; D013752:Tetracycline