Mast cell growth factor maps near the steel locus on mouse chromosome 10 and is deleted in a number of steel alleles.
Many spontaneous, chemical-induced, and radiation-induced dominant white spotting (W) and steel (Sl) mutations have been identified in the mouse. W and Sl mutations have similar phenotypic effects including deficiencies in pigment cells, germ cells, and blood cells, Numerous studies have suggested that W acts within the affected cell while Sl instead exerts its effects in the extracellular environment. Recent findings demonstrating that W encodes the c-kit proto-oncogene, a tyrosine kinase membrane receptor, have suggested that Sl encodes a ligand for c-kit. In the accompanying article we report the identification and purification of mast cell growth factor (MGF), a c-kit ligand. Here we describe the cloning of sequences encoding MGF. Furthermore, we show that Mgf maps near Sl in the distal region of mouse chromosome 10 and is deleted in a number of Sl alleles. These findings strongly support the notion that Sl encodes the mast cell growth factor.
Mammalian Genetics Laboratory, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
N G
NG
Copeland
D J
DJ
Gilbert
B C
BC
Cho
P J
PJ
Donovan
N A
NA
Jenkins
D
D
Cosman
D
D
Anderson
S D
SD
Lyman
D E
DE
Williams
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005799:Genes, Dominant; D008040:Genetic Linkage; D016298:Hematopoietic Cell Growth Factors / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D019089:Stem Cell Factor
D016298:Hematopoietic Cell Growth Factors; D015345:Oligonucleotide Probes; D019089:Stem Cell Factor; D004247:DNA