Carbohydrates inhibit the potentiating effect of bacteria, endotoxin and virus on basophil histamine release.
Histamine release caused by calcium ionophore A23187 and anti-IgE was examined in leukocyte suspensions from 8 healthy individuals. Staphylococcus aureus, lipopolysaccharide (LPS) from Salmonella typhimurium and influenza A virus were found to enhance the histamine release but did not release histamine per se. The potentiation of mediator release depends on a non-transient signal since the potentiating effect was also obtained by preincubation of the cells with LPS followed by wash-out and stimulation of the cells with anti-IgE. The potentiation was abolished or reduced by galactose, N-acetyl-glucosamine, alpha-methyl-D-glucoside, alpha-methyl-D-mannoside, N-acetylneuraminic acid and lactose, but not by glucose. These findings indicate that the enhancement of mediator release by bacteria, endotoxin, and virus depends on a sugar-mediated reaction.
Department of Pharmacology, University of Copenhagen, Denmark.
S
S
Norn
P
P
Clementsen
K S
KS
Kristensen
C
C
Hannoun
J O
JO
Jarløv
D001491:Basophils / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology*; D004731:Endotoxins / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D066298:In Vitro Techniques; D009980:Influenza A virus / Q000502:physiology; D012486:Salmonella typhimurium / Q000502:physiology; D013211:Staphylococcus aureus / Q000502:physiology
D002241:Carbohydrates; D004731:Endotoxins; D007074:Immunoglobulin G; D000001:Calcimycin