Activation of neurons in the rostral ventrolateral medulla increases bronchomotor tone in dogs.
Previous work from this laboratory has demonstrated that the chemical activation of cell bodies in the caudal ventrolateral medulla of chloralose-anesthetized dogs decreased bronchomotor tone by withdrawing cholinergic input to airway smooth muscle. In the present study we determined the bronchomotor responses to microinjection of DL-homocysteic acid (100 mM; 25-50 nl) into the rostral ventrolateral (RVL) medulla of chloralose-anesthetized dogs. Total lung resistance was used as a functional index of bronchomotor tone. Microinjection of DL-homocysteic acid into the 20 sites located in the lateral aspect of the RVL medulla increased both total lung resistance [from 6.5 +/- 0.4 to 9.1 +/- 0.8 (SE) cmH2O.l-1.s; P less than 0.05] and mean arterial pressure (from 125 +/- 5 to 148 +/- 8 mmHg; P less than 0.05). Microinjection of this amino acid into nine sites located in the medial aspect of the RVL medulla increased mean arterial pressure (from 130 +/- 6 to 153 +/- 6 mmHg; P less than 0.05) but had no effect on total lung resistance. We confirmed in three sites that the increase in total lung resistance evoked by microinjection of DL-homocysteic acid was accompanied by an increase in tracheal smooth muscle tension. The increase in total lung resistance evoked by DL-homocysteic acid was not affected by beta-adrenergic blockade but was abolished by muscarinic blockade.
Division of Cardiovascular Medicine, University of California, Davis 95616.
J R
JR
Haselton
P A
PA
Padrid
M P
MP
Kaufman
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000403:Airway Resistance / Q000187:drug effects; D000818:Animals; D001784:Blood Gas Analysis; D001980:Bronchi / Q000166:cytology / Q000294:innervation*; D004285:Dogs; D006710:Homocysteine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D008012:Lidocaine / Q000494:pharmacology; D008170:Lung Compliance / Q000187:drug effects; D008526:Medulla Oblongata / Q000166:cytology / Q000502:physiology*; D008845:Microinjections; D009119:Muscle Contraction / Q000502:physiology; D009129:Muscle Tonus / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D010275:Parasympathetic Nervous System / Q000502:physiology; D010276:Parasympatholytics / Q000494:pharmacology; D013238:Stereotaxic Techniques
D000319:Adrenergic beta-Antagonists; D010276:Parasympatholytics; D006710:Homocysteine; C007956:homocysteic acid; D008012:Lidocaine