An intron in the genes for U3 small nucleolar RNAs of the yeast Saccharomyces cerevisiae.
The origin of the intervening sequences (introns), which are removed during RNA maturation, is currently unknown. They are found in most genes encoding messenger RNAs, but are lacking in almost all small nuclear (sn)RNAs. One exceptional snRNA (U6) is part of the spliceosomal machinery that is involved in messenger RNA maturation. It has been suggested that its intron arose as a result of incorrect splicing of a messenger RNA precursor. This study revealed the presence of an intron, with the characteristic features of nuclear introns from precursors to messenger RNA, in the two genes coding for Saccharomyces cerevisiae U3 snRNA. The branch point was GACTAAC instead of the TACTAAC sequence found in all yeast introns examined so far. As U3 is a nucleolar snRNA required for maturation of ribosomal RNA, its intron could not have been acquired from aberrant messenger RNA processing in a spliceosome.
Laboratoire d'Enzymologie et de Génie Génétique, Université de Nancy, France.
E
E
Myslinski
V
V
Ségault
C
C
Branlant
D001483:Base Sequence; D007438:Introns; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012326:RNA Splicing; D012331:RNA, Fungal / Q000235:genetics*; D012342:RNA, Small Nuclear / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic
D012331:RNA, Fungal; D012342:RNA, Small Nuclear; D012194:RNA-Directed DNA Polymerase