Amplification by the polymerase chain reaction of a specific target sequence in the gene coding for Escherichia coli verotoxin (VTe variant).
Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) protocol to target a specific sequence in the gene coding for the A subunit of Escherichia coli verotoxin (VTe-variant, VTev). This PCR protocol permits the VTe-variant target sequence to be distinguished from closely related sequences in the same coding regions for type 1, type 2, and type 2 variant E. coli verotoxins. This procedure will be a valuable adjunct to other DNA amplification techniques currently being used for molecular epidemiological studies of verotoxigenic E. coli.
Johnson
W M
WM
National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.
Tyler
S D
SD
Wang
G
G
Lior
H
H
D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D003854:Deoxyribonucleotides; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D003854:Deoxyribonucleotides; D022622:Shiga Toxin 1