Angiogenic activity in damaged skeletal muscle.
After a muscle is damaged, blood vessels spontaneously grow into the injured region as the muscle fibers regenerate. The stimulus for this vascular ingrowth is currently unknown. We hypothesized that the damaged muscle releases a factor(s) capable of stimulating this revascularization. To test this theory, extracts were prepared from rabbit hind limb muscles and incorporated into Hydron, a slow-release polymer. Pellets of the extract containing Hydron were implanted between the layers of the rabbit corneal stroma as an assay for angiogenic activity. The normally avascular corneas were examined 7 days after surgery for the presence of new blood vessels. Skeletal muscle-derived extract from rabbits elicited positive angiogenic responses in a dose-dependent manner. Four hundred to 500 micrograms of the skeletal muscle-derived extract were required to produce maximum vessel ingrowth. The control, Dulbecco's phosphate-buffered saline in Hydron, failed to stimulate neovascularization.
Department of Surgery, University of Minnesota, Minneapolis 55455.
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Phillips
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Knighton
D043925:Angiogenesis Inducing Agents / Q000502:physiology*; D000818:Animals; D001681:Biological Assay; D003315:Cornea / Q000098:blood supply; D005260:Female; D006133:Growth Substances / Q000502:physiology*; D020100:Hydrogels; D009132:Muscles / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation; D009389:Neovascularization, Pathologic; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives; D019736:Prostheses and Implants; D011817:Rabbits
D043925:Angiogenesis Inducing Agents; D006133:Growth Substances; D020100:Hydrogels; C000257:polyhydroxyethyl methacrylate hydrogels; D011102:Polyhydroxyethyl Methacrylate