Simplified northern blot hybridization using 5% sodium dodecyl sulfate.
We describe a simplified Northern blot hybridization procedure. This procedure eliminates the need for many reagents commonly used in RNA hybridization and replaces them with two buffers containing 5% sodium dodecyl sulfate for pre-hybridization, hybridization and all post-hybridization washes.
Virca
G D
GD
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Northemann
W
W
Shiels
B R
BR
Widera
G
G
Broome
S
S
D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D015342:DNA Probes; D051379:Mice; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis; D012967:Sodium Dodecyl Sulfate
D015342:DNA Probes; D012967:Sodium Dodecyl Sulfate; D012313:RNA