Tyrosine hydroxylase-like immunoreactive neurons in the olfactory bulb of the snake, Elaphe quadrivirgata, with special reference to the colocalization of tyrosine hydroxylase- and GABA-like immunoreactivities.
The distribution and structural features of tyrosine hydroxylase-like immunoreactive (TH-LI) neurons were studied in the olfactory bulb of a snake, Elaphe quadrivirgata, by using pre- and post-embedding immunocytochemistry at the light microscopic level. In contrast to rodent olfactory bulbs previously reported, many TH-LI neurons were seen not only in the main olfactory bulb (MOB) but also in the accessory olfactory bulb (AOB). With regard to the TH-like immunoreactivity, there appeared no appreciable differences between MOB and AOB. As in mammalian MOB, the majority of TH-LI neurons were clustered in the periglomerular region and appeared to send their dendritic branches into glomeruli, which as a whole make an intense TH-LI band in the glomerular layer (GML). In the external plexiform/mitral cell layer (EPL/ML) of MOB and AOB as well as in the outer sublamina of the internal plexiform layer (OSL) of AOB, an appreciable number of TH-LI neurons were scattered, extending dendritic processes which appeared to make a loose meshwork. TH-LI neurons in EPL/ML (including OSL) appeared to consist of at least two morphologically different types. The first had a small perikaryon and one or two smooth dendrites which usually extended to GML and were frequently confirmed to enter into glomeruli. The second had a larger perikaryon and 2-3 dendrites which branched into several varicose processes extending in EPL/ML/OSL but appeared not to enter into glomeruli. The TH-like immunoreactivity was rarely seen in the internal plexiform layer and internal granule cell layer. The colocalization of GABA-like and TH-like immunoreactivities was further studied. Almost all TH-LI neurons in both EPL/ML/OSL and GML contained GABA-like immunoreactivity irrespectively of the type of TH-LI cells.
Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
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Kosaka
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Kosaka
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Nagatsu
D000818:Animals; D000911:Antibodies, Monoclonal; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology*; D009830:Olfactory Bulb / Q000166:cytology* / Q000201:enzymology; D012911:Snakes; D013687:Telencephalon / Q000166:cytology / Q000201:enzymology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D000911:Antibodies, Monoclonal; D005680:gamma-Aminobutyric Acid; D014446:Tyrosine 3-Monooxygenase