Stimulation of the absorption of macromolecules in Giardia lamblia infected mice intestine.
The absorption of 125I-labelled bovine serum albumin, gamma-globulin and alpha-lactalbumin was considerably enhanced in G. lamblia infected Swiss mice intestine compared to uninfected controls. The binding of 125I-proteins to brush border membrane was however, significantly (P less than 0.01) low in infected animals. Kinetic studies with gamma-globulin binding to brush borders revealed a decrease in the number of binding sites in infected animals (1.52) compared to controls (2.86 micrograms/mg protein) with no change in the affinity constant (47.60 micrograms/ml) under these conditions. These findings suggest that G. lamblia infection in mice leads to enhanced macromolecular absorption which seems unrelated to the binding of proteins to epithelial cell surface.
Department of Experimental Medicine, Postgraduate Institute of Medical Education & Research, Chandigarh.
S
S
Mahmood
N K
NK
Ganguly
R C
RC
Mahajan
B N
BN
Walia
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005873:Giardiasis / Q000378:metabolism*; D007408:Intestinal Absorption; D007768:Lactalbumin / Q000378:metabolism*; D008297:Male; D051379:Mice; D008871:Microvilli / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D005719:gamma-Globulins / Q000378:metabolism*
D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D007768:Lactalbumin