Enhanced chemiluminescence for tissue antigen and cellular viral DNA detection.
The ability to use enhanced chemiluminescence (ECL) to detect horseradish peroxidase as a label for tissue antigens and cellular viral DNA was demonstrated. A liquid nitrogen-cooled charged-coupled device (CCD) was used to detect light output, which was visualized on a monitor or was quantitated using an attached microcomputer. In a tissue antigen model, equivalent sensitivity was observed between ECL and colorimetric detection.
Hawkins
E
E
Corporate Research, Amersham International, Buckinghamshire, United Kingdom.
Cumming
R
R
D000951:Antigens, Neoplasm / Q000032:analysis*; D002460:Cell Line; D003124:Colorimetry; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D008165:Luminol; D027383:Papillomaviridae / Q000032:analysis; D013194:Staining and Labeling; D002583:Uterine Cervical Neoplasms / Q000032:analysis
D000951:Antigens, Neoplasm; D004279:DNA, Viral; D008165:Luminol