Effect of 5-aza-2'-deoxycytidine and retinoic acid on differentiation and c-myc expression in HL-60 myeloid leukemic cells.
The antineoplastic effects of 5-aza-2'-deoxycytidine (5-AZA-CdR) and retinoic acid on human HL-60 myeloid leukemic cells were investigated. 5-AZA-CdR or retinoic acid reduced the clonogenicity and induced the differentiation of HL-60 leukemic cells. The effects produced by 5-AZA-CdR and retinoic acid in combination on clonogenicity and differentiation of HL-60 leukemic cells were additive. These agents in combination also produced an additive decrease in the mRNA expression of the c-myc. These data indicate that 5-AZA-CdR and retinoic acid in combination produce an additive antineoplastic effect against HL-60 human myeloid leukemic cells.
Momparler
R L
RL
Departement de Pharmacologie, Université de Montréal, Québec, Canada.
Doré
B T
BT
Momparler
L F
LF
D000970:Antineoplastic Agents; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D000077209:Decitabine; D004357:Drug Synergism; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D016271:Proto-Oncogene Proteins c-myc; D014212:Tretinoin; D000077209:Decitabine; D001374:Azacitidine