Quantification of acute phase proteins in rat serum and in the supernatants of a cultured rat hepatoma cell line and cultured primary hepatocytes by an enzyme-linked immunosorbent assay.
An enzyme-linked immunosorbent assay (ELISA) has been developed to measure acute phase reactants (APRs) in rat serum. Rat APRs of alpha 2-macroglobulin, alpha 1-acid glycoprotein (AGP), haptoglobin (Hp), haemopexin (Hpx), cysteine protease inhibitor (CPI), albumin (Alb) and transferrin (Tf) were purified from the plasma of turpentine treated rats. Fab' fragments from each IgG fraction were labelled with horseradish peroxidase (POD) by the maleimide method and the assay was performed using a sandwich technique. This ELISA procedure was able to measure nanogram quantities of rat serum APRs.
Meiji Institute of Health Science, Kanagawa, Japan.
M
M
Ikawa
Y
Y
Shozen
D000209:Acute-Phase Proteins / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D014425:Turpentine
D000209:Acute-Phase Proteins; D000906:Antibodies; D003470:Culture Media; D007375:Interleukin-1; D015850:Interleukin-6; D014425:Turpentine