Detection of T1-oligonucleotides of the foot-and-mouth disease virus RNA [correction of DNA] by silver staining.
Viral (v) RNA was isolated from purified foot-and-mouth disease virus (FMDV) by phenol-chloroform-isoamylalcohol treatment, digested by RNase T1 and separated by one-dimensional polyacrylamide gel electrophoresis (PAGE). The oligonucleotides were detected by silver staining. About 45 micrograms of vRNA corresponding to about 100 ml of infectious BHK-21 cell culture fluid yielded a pattern of nearly 20 bands sufficient to differentiate between the FMD viruses.
VEB Friedrich-Loeffler-Institut, Isle of Riems, G.D.R.
H
H
Liebermann
C
C
Fedtke
W
W
Kwasniowski
D000818:Animals; D005537:Aphthovirus / Q000235:genetics*; D002478:Cells, Cultured; D006224:Cricetinae; D005536:Foot-and-Mouth Disease / Q000235:genetics; D009841:Oligonucleotides / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D006163:Ribonuclease T1; D012834:Silver; D013194:Staining and Labeling
D009841:Oligonucleotides; D012367:RNA, Viral; D012834:Silver; D006163:Ribonuclease T1