Effects of antiallergic drugs on superoxide anion generation from activated human alveolar macrophages measured by chemiluminescence method.
Human alveolar macrophages were recovered by bronchoalveolar lavage and superoxide anion (O2-) generation from the macrophages were measured by 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo[1,2-a]pyrazin++ +-3-one (MCLA)-dependent luminescence. Addition of 0.5 mumol/l MCLA and a stimulatory agent, such as phorbol myristate acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP), to a suspension of the cells caused a marked luminescence which was inhibited by 0.5 mumol/l superoxide dismutase (SOD). The maximal luminescence intensity was dependent upon the number of the cells. Azelastine (A-5610, CAS 58581-89-8) significantly inhibited the O2- generation from the activated macrophages in a dose-dependent manner. However, the other antiallergic drugs, such as ketotifen, disodium cromoglycate and others showed little effects on the O2- generation from the activated human alveolar macrophages.
First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
M
M
Kurosawa
T
T
Ishizuka
S
S
Kobayashi
M
M
Nakano
D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004205:Cromolyn Sodium / Q000494:pharmacology; D006633:Histamine Antagonists / Q000494:pharmacology*; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D006801:Humans; D007093:Imidazoles; D066298:In Vitro Techniques; D007665:Ketotifen / Q000494:pharmacology; D008163:Luminescent Measurements; D008264:Macrophages / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D010793:Phthalazines / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism; D011719:Pyrazines; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D006633:Histamine Antagonists; D006635:Histamine H2 Antagonists; D007093:Imidazoles; D010793:Phthalazines; D011719:Pyrazines; D013481:Superoxides; C063412:2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-alpha)pyrazin-3-one; D013482:Superoxide Dismutase; D004205:Cromolyn Sodium; D007665:Ketotifen; C020976:azelastine