A high-affinity human monoclonal IgM antibody reacting with multiple strains of Mycoplasma hominis.
Human monoclonal antibodies were produced against Mycoplasma hominis by in vitro immunization of peripheral blood lymphocytes from a healthy seropositive donor using low amounts of antigen (5 ng/ml). The immune B lymphocytes were subsequently immortalized by Epstein-Barr virus transformation followed by somatic cell fusion. Using this procedure, several specific anti-M. hominis monoclonal antibodies of mu isotype were obtained exhibiting apparent affinity constants as high as 3 x 10(9)M-1. The productivity of a number of different hybridomas was determined to be in the range 7-22 micrograms x (24 hr x 10(6) cells)-1. The specificity of one antibody, 129.1, was further characterized by enzyme-linked immunosorbent assay against several different species of Mycoplasma and by Western blot analysis. The antibody specifically bound to a protein of Mr 100,000 present in 10 of 12 different strains of M. hominis.
Department of Immunotechnology, Lund University, Sweden.
S A
SA
Möller
S
S
Birkelund
C A
CA
Borrebaeck
D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000915:Antibody Affinity / Q000276:immunology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D009174:Mycoplasma / Q000276:immunology*; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D007074:Immunoglobulin G