Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis.
Normal epithelia and carcinomas of the human uterine cervix were studied by monoclonal antibodies chain specific for cytokeratins 4, 8, 10, 13, 14, 18, and 19. Most cells in 13 examined squamous carcinomas revealed a cytokeratin phenotype detected in ectocervical basal cells and endocervical subcolumnar reserve cells: 8+, 14+, 18+, 19+, 4-, 10-, 13-. We propose that these two cell types are closely related or identical and that squamous carcinoma of the cervix originates in this cell type. In more differentiated tumor cells cytokeratins 4, 10, and 13, which are present in suprabasal layers of the normal ectocervical epithelium, were coexpressed with basal cell cytokeratins. Thus, contrary to previous beliefs, all cytokeratins detected in carcinomas were also present in normal epithelium of uterine cervix. The cytokeratin profile of cervical adenocarcinomas corresponded to that of columnar endocervical cells (8+, 18+, 19+), although two of the three adenocarcinomas also expressed cytokeratin 4, which in the normal endocervix was detected in scattered single columnar cells only. The new monoclonal antibody DE-K14, specific for cytokeratin 14, proved a specific marker of subcolumnar reserve cells in the endocervix. It was also the only one that reacted with all cervical squamous carcinomas but with none of the cervical adenocarcinomas and, as such, has a potential value for pathological differential diagnosis of cervical tumors.
Division of Tumor Biology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Huis, Amsterdam.
D
D
Ivanyi
E
E
Groeneveld
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G
Van Doornewaard
W J
WJ
Mooi
P C
PC
Hageman
D000230:Adenocarcinoma / Q000032:analysis / Q000473:pathology; D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell / Q000032:analysis / Q000473:pathology; D002584:Cervix Uteri / Q000032:analysis / Q000166:cytology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007633:Keratins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D012016:Reference Values; D002583:Uterine Cervical Neoplasms / Q000032:analysis / Q000473:pathology*
D000911:Antibodies, Monoclonal; D007633:Keratins