Beta-adrenergic treatment of C6 glioma cells produces opposite changes in c-fos and c-jun mRNA levels.
The AP1 transcriptional complex is a heterodimer composed of proteins encoded by the fos and jun proto-oncogene families. Changes in the concentration and composition of AP1 occur after cells are perturbed in a variety of different ways (Curran, in Reddy et al., eds. "The Oncogene Handbook," Amsterdam: Elsevier, pp 307-325, 1988; Sonnenberg et al., Neuron 3:359-365, 1989). Transient changes in AP1 content presumably result in altered expression of AP1-regulated target genes, that help to mediate the cell's long-term response to changes in its environment. One factor that may be important in determining which target genes are regulated by AP1 in a given context is the identity of the jun family member present in the complex (Chiu et al., Cell 59:979-986, 1989; Schutte et al., Cell 59:987-997, 1989). Fos induction has been demonstrated after binding of beta-adrenergic ligands to their cell surface receptors (Barka et al., Mol Cell Biol 6:2984-2989, 1986; Gubits et al., Mol Brain Res 6: 39-45, 1989; Arenander et al., J Neurosci Res 24: 107-114, 1989; Mocchetti et al., Proc Natl Acad Sci USA 86:3891-3895, 1989). However, the response of the jun gene family to this treatment has not been reported. We have therefore examined the effect of beta-adrenergic receptor activation on the expression of c-fos, c-jun, and junB mRNA levels in C6 glioma cells. Our results indicate that c-fos and junB mRNA levels are increased by 52- and 2.7-fold, respectively, after 45 min of isoproterenol (IPR) treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
Gubits
R M
RM
Department of Neurology and Pathology, College of Physicians and Surgeons of Columbia University, New York, New York 10032.
Yu
H
H
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D015152:Blotting, Northern; D004305:Dose-Response Relationship, Drug; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D007545:Isoproterenol / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D016761:Oncogene Proteins v-fos / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000318:Adrenergic beta-Agonists; D016761:Oncogene Proteins v-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D007545:Isoproterenol