An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels.
A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels are applied to gradient gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.
Instituto Oswaldo Cruz, Departamento de Bioquímica e Biologia Molecular, Rio de Janeiro, Brasil.
A M
AM
Gonçalves
N S
NS
Nehme
C M
CM
Morel
D000818:Animals; D004270:DNA, Circular / Q000032:analysis*; D018105:DNA, Kinetoplast; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D013194:Staining and Labeling; D014351:Trypanosomatina / Q000145:classification*
D004270:DNA, Circular; D018105:DNA, Kinetoplast