Olfactory neuroblastoma. Additional immunohistochemical characterization.
A panel of 12 antibodies was used to further characterize the immunohistochemical staining profile of olfactory neuroblastoma. The following results were obtained for the 11 neoplasms that were immunostained: neuron-specific enolase 11/11(+), S-100 protein 8/11(+), microtubule-associated protein-2 8/11(+), class III beta-tubulin isotype 9/11(+), neurofilament 200 kD 8/11(+), synaptophysin 7/11(+), glial fibrillary acidic protein 1/11(+), chromogranin A 1/11(+), vimentin 1/11(+), keratin (CAM 5.2) 4/11(+), keratin (AEI/AE3) 0/11(+), and epithelial membrane antigen 0/11(+). Expression of two intermediate filaments was found in 4 of the 11 tumors. The authors' data showing that 72% of olfactory neuroblastomas were S-100 protein positive and only one was immunoreactive for glial fibrillary acidic protein agree with other published immunohistochemical studies. With only a single exception, each of the 11 neoplasms was labeled with one or more antibodies that detect neuronal cytoskeletal proteins (class III beta-tubulin isotype, microtubule-associated protein-2, neurofilament 200 kD). These immunohistochemical results are complementary to the reported electron microscopic findings of intermediate filaments and microtubules in olfactory neuroblastomas.
Department of Pathology, University of Virginia, Health Sciences Center, Charlottesville 22908.
H F
HF
Frierson
G W
GW
Ross
S E
SE
Mills
A
A
Frankfurter
D000328:Adult; D000368:Aged; D053379:Chromogranin A; D002864:Chromogranins / Q000378:metabolism; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008875:Middle Aged; D018396:Mucin-1; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000378:metabolism* / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D016708:Synaptophysin; D014404:Tubulin / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D053379:Chromogranin A; D002864:Chromogranins; D005904:Glial Fibrillary Acidic Protein; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D018396:Mucin-1; D009418:S100 Proteins; D016708:Synaptophysin; D014404:Tubulin; D014746:Vimentin; D007633:Keratins; D010751:Phosphopyruvate Hydratase