Purification of gramicidin A.
A simple chromatographic purification of the naturally occurring ion channel-forming pentadecapeptide gramicidin A (gA) is presented. This procedure allows gA to be isolated in gram quantities from the commercially available mixture of isomers after chromatography on silica gel. The gramicidin A obtained in this manner is greater than 95% pure as determined by 1HNMR, HPLC, and amino acid analysis.
Department of Chemistry, Yale University, New Haven, Connecticut 06511.
C J
CJ
Stankovic
J M
JM
Delfino
S L
SL
Schreiber
D002625:Chemistry, Organic / Q000379:methods; D002845:Chromatography / Q000379:methods; D006096:Gramicidin / Q000302:isolation & purification*; D058428:Silica Gel; D012822:Silicon Dioxide
D006096:Gramicidin; D058428:Silica Gel; D012822:Silicon Dioxide