Kaliuretic response to potassium loading in amiloride-treated dogs.
To assess whether an intact mechanism of sodium transport in the distal nephron is a prerequisite for the development of a kaliuresis in response to an acute potassium load (0.4 M KCl, 1 ml/min), the effects of a simultaneous infusion of KCl and amiloride (1 mg/kg/h) were evaluated in anesthetized dogs. A major reduction in potassium excretion mainly due to a sharp decrease in urine K+ concentration to one tenth of control levels was found after amiloride. The simultaneous infusion of KCl and amiloride resulted in a rapid and major increase in kaliuresis that was accounted for mostly by the rise in urine K+ concentration. The increased kaliuresis after the acute potassium infusion was of similar magnitude when expressed as percent value of control to that previously reported in dogs not receiving amiloride; the absolute rates of K+ excretion, however, were only 2.7 and 7.3% (before and after KCl infusion, respectively) of the values in dogs not receiving amiloride. Our observations suggest that potassium infusion in the intact dog increases kaliuresis primarily as a result of a more favorable chemical gradient of this cation between blood and/or distal tubular cells and urine. Yet, when a chemical gradient is the only driving force of potassium secretion, as was the case in our amiloride-treated dogs, the absolute rate of kaliuresis is very modest. The presence of an unimpaired electrical profile and sodium transport mechanisms in the distal nephron, although not critical for the development of kaliuresis in response to a K+ load, accounts for a severalfold rise in renal potassium excretion above basal levels.
Yeyati
N L
NL
Renal Section, Hospital de Clinicas, Universidad de Buenos Aires, Argentina.
Etcheverry
J C
JC
Adrogué
H J
HJ
D000042:Absorption; D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D003900:Desoxycorticosterone / Q000494:pharmacology; D004285:Dogs; D007668:Kidney / Q000378:metabolism*; D007672:Kidney Cortex / Q000648:ultrastructure; D007685:Kidney Tubules, Collecting / Q000648:ultrastructure; D007686:Kidney Tubules, Distal / Q000166:cytology; D008566:Membranes / Q000378:metabolism; D009399:Nephrons / Q000378:metabolism; D011188:Potassium / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine*; D011292:Premedication; D012964:Sodium / Q000493:pharmacokinetics; D015222:Sodium Channels / Q000187:drug effects
D015222:Sodium Channels; D003900:Desoxycorticosterone; D000584:Amiloride; D012964:Sodium; D011188:Potassium