Differential keratin gene expression during the differentiation of the cement gland of Xenopus laevis.
The expression of both epidermal and nonepidermal keratins has been detected in the cement gland of Xenopus laevis by antibody staining. Northern blot and in situ hybridizations with gene-specific probes indicated the expression of the nonepidermal keratin, XK endo B, and the embryonic epidermal keratin, XK70, in the cement gland. Furthermore, since explanted animal pole cells can be induced to differentiate into cement gland cells in vitro by incubation in NH4Cl, we have demonstrated the in vitro induction of XK endo B, maintenance of XK70, and repression of another embryonic epidermal keratin, XK81. This is the first report of keratin gene expression in the cement gland.
Laboratory of Molecular Genetics, National Institute of Child Health and Development, National Institutes of Health, Bethesda, Maryland 20892.
S E
SE
LaFlamme
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IB
Dawid
D000818:Animals; D002454:Cell Differentiation; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000196:embryology*; D005786:Gene Expression Regulation; D007633:Keratins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012627:Sebaceous Glands / Q000166:cytology / Q000196:embryology*; D014982:Xenopus laevis
D012333:RNA, Messenger; D007633:Keratins