Use of probes to detect virulence factor DNA sequences in Escherichia coli strains isolated from foods.
Escherichia coli strains were isolated from 96 food samples (32 milks, 4 dairy products, 36 raw meats, 7 meat products, 7 sandwiches and 10 ready-to-eat meals). A total of 306 colonies was submitted to hybridization assays with DNA probes for the following virulence factors: heat-labile toxins (LT-I and LT-II), heat-stable toxins (ST-h and ST-p). Shiga-like toxins (SLT-I and SLT-II), adherence factor of enteropathogenic E. coli (EAF) and invasive factor (INV). Six colonies isolated from 4 food samples hybridized with the probes for LT-II (3 colonies isolated from a milk sample), SLT-I and SLT-II (1 colony isolated from raw bovine meat) or EAF (2 colonies isolated from two raw chicken meat samples).
Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.
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Franco
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Gomes
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Jakabi
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Marques
D018830:Adhesins, Escherichia coli; D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000235:genetics; D001427:Bacterial Toxins / Q000032:analysis / Q000235:genetics; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D003611:Dairy Products; D004768:Enterotoxins / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D005516:Food Microbiology; D008460:Meat; D008461:Meat Products; D008892:Milk; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D014774:Virulence
D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D015342:DNA Probes; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; C034151:heat stable toxin (E coli); C027538:heat-labile enterotoxin, E coli