Exclusion of linkage between familial Mediterranean fever and the human serum amyloid A (SAA) gene cluster.
We studied the relationship between the autosomal recessive trait familial Mediterranean fever (FMF) and the serum amyloid A (SAA) genes by comparing alleles of a highly polymorphic dinucleotide repeat and a conventional restriction fragment length polymorphism (RFLP) in the SAA gene cluster in Israeli FMF kindreds. By haplotype analysis, our data indicate a minimum crossover frequency of 22% between the SAA gene marker and FMF. By conventional linkage analysis this eliminates a minimum of 10.4 cM including and surrounding the SAA gene cluster as the site of the FMF mutation although SAA proteins are prominent physiologic markers of the acute attacks.
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.
G H
GH
Sack
C C
CC
Talbot
B G
BG
McCarthy
E L
EL
Harris
D
D
Kastner
L
L
Gruberg
M
M
Pras
D003434:Crossing Over, Genetic; D010505:Familial Mediterranean Fever / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D008126:Lod Score; D008297:Male; D005810:Multigene Family; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D000685:Serum Amyloid A Protein / Q000235:genetics*
D000685:Serum Amyloid A Protein