[Study of characteristics of monoclonal antibody against substance P].
Cross-reaction of a rat monoclonal antibody (BTP-1) against seventeen substance P analogues was studied. The antibody was of IgG type and related to the carboxyl terminal of substance P, especially methionyl in the terminal, but did not depend on the strength of antagonistic effects of these analogues. It did not show cross-reaction with the following nine peptides: glucagon, endorphin, angiotensin I, II, leucine-enkephalin, methionine-enkephalin, bradykinin, oxytocin and dernorthin, indicating its high specificity to substance P. By means of immuno-enzyme histochemical method, it was shown that stained nerve fibers were located in the gelaliternous substance of Rolando, interpeduncular nucleus, substantia nigra and nerve cell bodies in the vestibular nucleus, lateral tegmental nucleus of mesencephalon and ventral region of third ventricle.
Shanghai Center of Biotechnology, Academia Sinica.
D H
DH
Chen
C X
CX
Tong
J M
JM
Zhang
J
J
Zhang
X R
XR
Ge
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D011903:Raphe Nuclei / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000032:analysis; D013373:Substance P / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology*; D013378:Substantia Nigra / Q000032:analysis
D000911:Antibodies, Monoclonal; D013373:Substance P