Immunodominant epitopes of the adhesin of Mycoplasma pneumoniae.
Overlapping octapeptides from the amino acid sequence of the adherence protein of Mycoplasma pneumoniae were synthesized and used as the antigen in an enzyme-linked immunosorbent assay with serum samples from M. pneumoniae-infected patients. Of a sequence of 338 amino acids positioned between leucine 801 and leucine 1139, only two regions with immunodominant continuous epitopes were detected. The immunoglobulin G and immunoglobulin M antibodies of child and adult patients reacted especially with the NH2-(810)-W-I-G-N-G-Y-R-Y peptide but also reacted with the NH2-(1124)-F-T-D-F-V-K-P-R peptide.
Institute for Medical Microbiology and Hygiene, University of Freiburg, Federal Republic of Germany.
E
E
Jacobs
A
A
Pilatschek
B
B
Gerstenecker
K
K
Oberle
W
W
Bredt
D018829:Adhesins, Bacterial; D000293:Adolescent; D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000276:immunology; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D011019:Pneumonia, Mycoplasma / Q000276:immunology
D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D007136:Immunoglobulins; D010455:Peptides; C035471:adhesin, Mycoplasma pneumoniae