Pseudomonas aeruginosa exoenzyme S is an adhesion.
Exoenzyme S from Pseudomonas aeruginosa has been studied as an adhesion for glycosphingolipids and buccal cells. Binding of exoenzyme S to gangliotriosylceramide (GalNAc beta 1-4Gal beta 1-4Glc beta 1-1Cer), gangliotetraosylceramide (Gal beta 1-3 GalNAcT beta 1-4 Gal beta 1-4Glc beta 1-1Cer), and lactosylceramide (Gal beta 1-4Glc beta 1-1Cer) separated on thin-layer chromatograms was observed. Binding curves for exoenzyme S with dilutions of gangliotetraosylceramide immobilized on plastic plates were similar to previously reported results for the intact bacteria. Binding of exoenzyme S to sialylated counterparts of these glycosphingolipids was not seen, indicating that the addition of a sialic acid residue interferes with binding. Exoenzyme S and monoclonal antibody to exoenzyme S inhibit the binding of P. aeruginosa to buccal cells. The presence of exoenzyme S on the surface of P. aeruginosa was detected by immunogold labeling of bacteria with antibodies to exoenzyme S. Results of these studies led us to conclude that exoenzyme S is an important adhesion of P. aeruginosa.
Department of Microbiology, Ohio State University, Columbus 43210.
N R
NR
Baker
V
V
Minor
C
C
Deal
M S
MS
Shahrabadi
D A
DA
Simpson
D E
DE
Woods
D036002:ADP Ribose Transferases; D018829:Adhesins, Bacterial; D000818:Animals; D015703:Antigens, CD; D001422:Bacterial Adhesion / Q000276:immunology; D001426:Bacterial Proteins / Q000378:metabolism*; D001427:Bacterial Toxins; D002240:Carbohydrate Sequence; D010861:Fimbriae, Bacterial / Q000378:metabolism; D005732:Gangliosides; D006028:Glycosphingolipids / Q000378:metabolism; D007150:Immunohistochemistry; D007790:Lactosylceramides; D037102:Lectins; D008969:Molecular Sequence Data; D011065:Poly(ADP-ribose) Polymerases / Q000276:immunology / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000276:immunology / Q000648:ultrastructure; D011817:Rabbits
D018829:Adhesins, Bacterial; D015703:Antigens, CD; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C011258:G(A1) ganglioside; D005732:Gangliosides; D006028:Glycosphingolipids; D007790:Lactosylceramides; D037102:Lectins; C038692:adhesin, Pseudomonas; C038244:ganglio-N-triaosylceramide; C009744:CDw17 antigen; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases; C444820:exoenzyme S