Lesion selectivity in blockage of lambda exonuclease by DNA damage.
Various kinds of DNA damage block the 3' to 5' exonuclease action of both E. coli exonuclease III and T4 DNA polymerase. This study shows that a variety of DNA damage likewise inhibits DNA digestion by lambda exonuclease, a 5' to 3' exonuclease. The processive degradation of DNA by the enzyme is blocked if the substrate DNA is treated with ultraviolet irradiation, anthramycin, distamycin, or benzo[a]-pyrene diol epoxide. Furthermore, as with the 3' to 5' exonucleases, the enzyme stops at discrete sites which are different for different DNA damaging agents. On the other hand, digestion of treated DNA by lambda exonuclease is only transiently inhibited at guanine residues alkylated with the acridine mustard ICR-170. The enzyme does not bypass benzo[a]-pyrene diol epoxide or anthramycin lesions even after extensive incubation. While both benzo[a]-pyrene diol epoxide and ICR-170 alkylate the guanine N-7 position, only benzo[a]-pyrene diol epoxide also reacts with the guanine N-2 position in the minor groove of DNA. Anthramycin and distamycin bind exclusively to sites in the minor groove of DNA. Thus lambda exonuclease may be particularly sensitive to obstructions in the minor groove of DNA; alternatively, the enzyme may be blocked by some local helix distortion caused by these adducts, but not by alkylation at guanine N-7 sites.
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CIBA-GEIGY, Environmental Health Center, Farmington, CT 06032.
D000609:Aminoacridines; D000876:Anthramycin / Q000494:pharmacology; D001483:Base Sequence; D001580:Benzopyrenes / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D004247:DNA / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004249:DNA Damage; D004214:Distamycins / Q000494:pharmacology; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology; D014466:Ultraviolet Rays; D014764:Viral Proteins
D000609:Aminoacridines; D001580:Benzopyrenes; D004214:Distamycins; D009588:Nitrogen Mustard Compounds; D014764:Viral Proteins; C091910:acridine mustard; D000876:Anthramycin; C030000:stallimycin; D004247:DNA; D005090:Exodeoxyribonucleases; C043586:exo protein, Bacteriophage lambda; D002945:Cisplatin