Immunobiological studies with mycobacterial ribonucleic acid-protein complex: Part II--Mechanism of protective immunity against experimental tuberculosis.
Passive transfer of protective antituberculous immunity against LD50 dose of M. tuberculosis H37Rv was found to be mainly mediated by immune T-cells harvested from spleens of donor mice immunized with Myc. RNA-P-FIA complexes as monitored by indices of percent survival, root specific lung weight, lung density and by bacterial enumeration from different organs. Treatment of immune T-cells with anti-Thy 1.2. monoclonal antibodies plus complement prior to passive transfer, completely abrogated its protective effect thereby confirming their protective nature. Passive transfer of immune sera as well as immune T + B cells did not induce any enhancement in protective immunity.
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
P
P
Pancholi
V K
VK
Vinayak
G K
GK
Khuller
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D005260:Female; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012329:RNA, Bacterial / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014376:Tuberculosis / Q000517:prevention & control*
D001426:Bacterial Proteins; D012329:RNA, Bacterial