Trypanosoma cruzi: cloning and expression of an antigen recognized by acute and chronic human chagasic sera.
Here we describe the characterization of a Trypanosoma cruzi DNA sequence (clone A13) that codes for a polypeptide recognized by IgM and IgG antibodies from sera of acute and congenital chagasic patients. Antibodies to A13 antigen are also detected in the sera of chronic patients with different clinical forms of Chagas' disease, but not in sera of patients with leishmaniasis or other parasitic diseases. The antigenic determinants encoded by clone A13 are found in amastigotes and trypomastigotes of several T. cruzi strains, but not in the noninfective epimastigotes. The DNA sequence of the recombinant clone reveals one open reading frame encoding 251 amino acids without tandemly repeated sequences. Our data suggest that the A13 antigen may be useful for the development of serodiagnostic procedures.
Instituto de Medicina Tropical da USP, SP, Brasil.
G S
GS
Paranhos
P C
PC
Cotrim
R A
RA
Mortara
A
A
Rassi
R
R
Corral
H L
HL
Freilij
S
S
Grinstein
J
J
Wanderley
M E
ME
Camargo
J F
JF
da Silveira
D000208:Acute Disease; D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D014355:Chagas Disease / Q000175:diagnosis / Q000276:immunology*; D002908:Chronic Disease; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014349:Trypanosoma cruzi / Q000235:genetics / Q000276:immunology*
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011994:Recombinant Proteins