Deficiency of kallikrein-like enzyme activities in cerebral tissue of patients with Alzheimer's disease.
We examined the changes in the intracerebral activities, at the time of postmortem autopsy, in patients with Alzheimer's disease. When compared with the control group, the activity of kallikrein-like enzyme was significantly decreased, while prolyl endopeptidase activity increased, in the patients group. Aprotinin inhibited 50% of the activity of the former enzyme at 2 x 10(-7) M. Taken together with the results of a multivariate study, the above findings may indicate that intracerebral kallikrein deficiency plays an important role in the pathogenesis of Alzheimer's disease.
Institute of Microbial Chemistry, Tokyo, Japan.
T
T
Aoyagi
T
T
Wada
M
M
Nagai
F
F
Kojima
S
S
Harada
T
T
Takeuchi
H
H
Takahashi
K
K
Hirokawa
T
T
Tsumita
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology*; D000595:Amino Acid Sequence; D007611:Aprotinin / Q000494:pharmacology; D001921:Brain / Q000201:enzymology*; D010450:Endopeptidases / Q000378:metabolism; D005260:Female; D006801:Humans; D007610:Kallikreins / Q000037:antagonists & inhibitors / Q000172:deficiency* / Q000378:metabolism; D007976:Leupeptins / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000378:metabolism; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases
D007976:Leupeptins; D007611:Aprotinin; D010450:Endopeptidases; D010447:Peptide Hydrolases; D007610:Kallikreins; D012697:Serine Endopeptidases; C000705287:PREPL protein, human; D000085822:Prolyl Oligopeptidases; C032854:leupeptin