Serotonin stimulates protein phosphorylation in the cestode Hymenolepis diminuta.
1. Serotonin stimulated the incorporation of 32P from [gamma-32P] ATP into crude membrane preparations (P2) of Hymenolepis diminuta in a dose-dependent manner (EC50 of approximately 0.79 microM). 2. This response was seen with several serotonin agonists, and was inhibited by several serotonin antagonists, which were identical to the previously described activation and inhibition of serotonin-sensitive adenylate cyclase. 3. Cyclic AMP produced a dose-dependent stimulation of 32P incorporation into the P2 fraction, with an EC50 of approximately 2.51 microM. 4. The targets for the serotonin stimulated incorporation of 32P were found to be in trypsin-labile proteins with Mr's of 134,000, 110,000, 82,000, 80,000 and 31,000.
Ribeiro
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Department of Biology, York University, Ontario, Canada.
Webb
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RA
D000818:Animals; D001345:Autoradiography; D000242:Cyclic AMP / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D006926:Hymenolepis / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000502:physiology*
D008565:Membrane Proteins; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D012701:Serotonin; D000242:Cyclic AMP