Enzyme-linked immunosorbent assay for the detection and the semi-quantitative determination of taxane diterpenoids related to taxol in Taxus sp. and tissue cultures.
An ELISA-assay for the detection and the semi-quantitative determination of taxane diterpenoids structurally related to taxol found in Taxus sp. has been developed. The antiserum was raised in rabbits using a 2'-succinyltaxol-bovine serum albumin conjugate as immunogen. The working range of the assay was from 1 to 100 ng of taxol. In order to improve the production of taxol, preliminary experiments have been performed on crude extracts of several Taxus sp.; the results indicate that the present immunoassay is an useful tool for the rapid screening of species, varieties or individual plants out of a large population as well as for tissue cultures analysis.
Jaziri
M
M
Université Libre de Bruxelles, Belgium.
Diallo
B M
BM
Vanhaelen
M H
MH
Vanhaelen-Fastre
R J
RJ
Zhiri
A
A
Bécu
A G
AG
Homes
J
J
D000470:Alkaloids / Q000032:analysis*; D046508:Culture Techniques; D004224:Diterpenes / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D017239:Paclitaxel; D010946:Plants, Medicinal / Q000032:analysis*
D000470:Alkaloids; D004224:Diterpenes; D017239:Paclitaxel