Demonstration and nature of endothelin-3-like immunoreactivity in somatostatin and choline acetyltransferase-immunoreactive nerve cells of the neostriatum of the rat.
Using a rabbit endothelin-1 (ET-1) antiserum together with a goat-anticholineacetylase antiserum or a mouse anti-somatostatin antiserum it was possible by means of double immunolabelling procedures to demonstrate ET-like immunoreactivity in striatal nerve cell bodies of the rat, which were shown to contain either cholineacetylase or somatostatin immunoreactivity. Absorption studies with ET-3, ET-1 or big ET-1 indicated that the ET-like immunoreactivity was ET-3 like. In agreement the radioimmunoassay showed that ET-3-like immunoreactivity was present in higher concentrations than ET-1-like immunoreactivity in the neostriatum and other brain areas. Characterization by reversed phase HPLC revealed, however, that a major portion of the neostriatal ET-3-like immunoreactivity was not identical to ET-3. The nature of neuronal ET in the rat may thus be more complicated than hitherto assumed.
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
K
K
Fuxe
B
B
Tinner
W
W
Staines
A
A
Hemsén
L
L
Hersh
J M
JM
Lundberg
D000818:Animals; D001923:Brain Chemistry; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D003342:Corpus Striatum / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology; D016232:Endothelins / Q000032:analysis*; D007106:Immune Sera; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000166:cytology* / Q000201:enzymology; D009928:Organ Specificity; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000032:analysis*
D016232:Endothelins; D007106:Immune Sera; D013004:Somatostatin; D002795:Choline O-Acetyltransferase