The acute and chronic effects of ethanol on cardiac muscle protein synthesis in the rat in vivo.
An investigation was made into the acute and chronic effects of ethanol on rates of protein synthesis in the hearts of young rats (80-100 g body weight). Acute ethanol administration (75 mmol/kg body weight, IP) significantly reduced the fractional rate of protein synthesis by 20% after 2.5 hr, compared with saline-treated controls. Chronic ethanol feeding (36% of total calories) for 6 weeks significantly reduced cardiac wet weight by 11%, when compared to rats fed isovolumetric amounts of the same diet in which ethanol was substituted by isocaloric glucose. Neither the concentration nor the content of mixed cardiac proteins relative to body weight were overtly altered by chronic ethanol feeding, although, the total content of mixed cardiac proteins were significantly decreased. RNA concentrations and RNA relative to body weight increased slightly, but total cardiac DNA decreased. Indices for the capacity or potential of the heart to synthesis protein (indicated by the RNA/protein and RNA/DNA ratios) and the "DNA-unit" (protein/DNA ratio) were increased in response to chronic ethanol treatment. The fractional and absolute rates of mixed protein synthesis in the heart were (relatively) unaltered by chronic ethanol treatment, as was RNA efficiency and synthesis relative to DNA. It was concluded that the heart displays contrasting responses to acute and chronic ethanol exposure.
Department of Clinical Biochemistry, Kings College School of Medicine and Dentistry, London, U.K.
V R
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Preedy
T J
TJ
Peters
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D000431:Ethanol / Q000633:toxicity*; D006321:Heart / Q000187:drug effects*; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis*; D009206:Myocardium / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D009124:Muscle Proteins; D000431:Ethanol; D012313:RNA; D004247:DNA