Repression of the alpha-fetoprotein gene promoter by progesterone and chimeric receptors in the presence of hormones and antihormones.
Using transient transfection assays, we showed that repression of the alpha-fetoprotein promoter by intact and deletion mutants of the progesterone receptor and by chimeric progesterone/glucocorticoid-estrogen receptors in the presence of their cognate hormones was closely correlated with their ability to bind to a progesterone/glucocorticoid-responsive element. This negative regulation was also observed in the presence of antihormones, providing evidence that receptor-antihormone complexes can bind to their responsive elements in vivo.
Unité 184 de Biologie Moléculaire et de Génie Génétique de l'Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine, Institut de Chimie Biologique, Strasbourg, France.
B
B
Turcotte
M E
ME
Meyer
M T
MT
Bocquel
L
L
Bélanger
P
P
Chambon
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens; D002678:Chimera; D002872:Chromosome Deletion; D005796:Genes; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D011374:Progesterone / Q000494:pharmacology*; D011397:Promegestone / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011980:Receptors, Progesterone / Q000235:genetics / Q000502:physiology*; D015183:Restriction Mapping; D013629:Tamoxifen / Q000031:analogs & derivatives / Q000494:pharmacology; D014162:Transfection; D000509:alpha-Fetoproteins / Q000235:genetics*
D015345:Oligonucleotide Probes; D011980:Receptors, Progesterone; D000509:alpha-Fetoproteins; D013629:Tamoxifen; D015735:Mifepristone; D011374:Progesterone; D011397:Promegestone