Secondary structure of the RNA component of a nuclear/mitochondrial ribonucleoprotein.
RNase mitochondrial RNA processing (MRP) is a site-specific endoribonuclease located in both the nucleus and mitochondria of vertebrate cells. The enzyme is a ribonucleoprotein whose RNA component has been shown to be encoded by a nuclear gene. Because RNase MRP is particular in its substrate requirement, RNA-RNA interaction has been proposed as important for the cleavage reaction. A secondary structure of this RNA from mouse cells has been derived by chemical modification of in vivo MRP RNA in ribonucleoprotein form, as isolated free RNA, and as RNA synthesized in vitro. Full-length MRP RNA appears to adopt a conformation containing a significant number of single-stranded residues and may form a pseudoknot. The data are consistent with both the RNA within the ribonucleoprotein and the free RNA possessing comparable secondary structures and suggest a possible site of interaction between enzyme and substrate. The human MRP RNA can be folded into a conformation very similar to that predicted for the mouse MRP RNA. A more limited analysis of human MRP RNA is consistent with the structure proposed for the mouse species.
Topper
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Department of Developmental Biology, Stanford University School of Medicine, California 94305-5427.
Clayton
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DA
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004722:Endoribonucleases / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D007624:KB Cells; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D000077278:RNA, Mitochondrial; D012261:Ribonucleoproteins / Q000378:metabolism*; D013045:Species Specificity
D015345:Oligonucleotide Probes; D000077278:RNA, Mitochondrial; D012261:Ribonucleoproteins; D012313:RNA; D004722:Endoribonucleases; C058330:mitochondrial RNA-processing endoribonuclease