The effects of an anti-CD18 antibody (R15.7) in antigen-induced airway hyperresponsiveness (AH) and cell influx in guinea pigs.
Aerosol ovalbumin challenge (OA) of sensitized guinea pigs induced airway hyperreactivity (AH) to i.v. acetylcholine (Ach) and serotonin (5-HT) 24 hr post OA. Bronchoalveolar lavage fluid 24 hrs after OA showed increased leukocytes compared to unsensitized unchallenged animals. Treatment with monoclonal antibody R15.7 (3 mg/kg i.v.,) 1 hr prior and 4 hours after OA prevented the induction of AH to Ach but not to 5-HT and reduced influx of leukocytes. We conclude: 1) antigen inhalation induces an increase in AH with an increase in proinflammatory cell influx and 2) treatment with anti-CD18 antibody inhibits cell influx and airway hyperreactivity.
Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT 06877.
T C
TC
Noonan
R H
RH
Gundel
S N
SN
Desai
C
C
Stearns
R W
RW
Barton
R
R
Rothlein
L G
LG
Letts
P J
PJ
Piper
D000818:Animals; D000906:Antibodies / Q000502:physiology*; D015703:Antigens, CD / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D018821:CD18 Antigens; D002464:Cell Migration Inhibition; D006168:Guinea Pigs; D007249:Inflammation / Q000276:immunology; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000276:immunology*
D000906:Antibodies; D015703:Antigens, CD; D018821:CD18 Antigens; D016028:Receptors, Leukocyte-Adhesion; D010047:Ovalbumin