[Different DNA forms in cell nuclei of the lamina epithelialis mucosa of small intestines of children with growth disturbances due to enteric disease].
From children with growth retardation due to enteric disease, duodenal jejunal biopsy material was investigated. Using a modified Feulgen-reaction--acridine orange staining after acid hydrolysis according to Fukuda et al. (1986)--different DNA forms could be demonstrated in nuclei of cells of the lamina epithelialis mucosae by fluorescence microscopy. These clear cut forms of DNA by different fluorescence colours were only found in altered mucosa. The findings were discussed with regard to the possibility of mitotic disorders and cancerogenesis.
Institut für Anatomie des Bereiches Medizin (Charité), Humboldt-Universität zu Berlin.
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Pfister
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Glede
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Schön
D002675:Child, Preschool; D004247:DNA / Q000032:analysis*; D004386:Duodenum / Q000473:pathology; D005767:Gastrointestinal Diseases / Q000150:complications*; D006130:Growth Disorders / Q000209:etiology / Q000473:pathology*; D006801:Humans; D007223:Infant; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling
D004247:DNA
Unterschiedliche DNS-Zustandsformen in Zellkernen der Lamina epithelialis mucosae des Dünndarmes bei Kindern mit enteral bedingten Gedeihstörungen.