Enhancement of interleukin 2 production in human and Gibbon T cells after in vitro treatment with lithium.
The effect of trace elements lithium, selenium, and zinc on interleukin 2 (IL-2) production by peripheral blood mononuclear cells (PBMC), MLA144, and Jurkat cell lines has been studied. Lithium markedly enhanced IL-2 production by MLA144 and PBMC, but not by Jurkat. Selenium could only enhance IL-2 production by MLA144, whereas in none of these three systems was IL-2 production altered by zinc. The enhancing effect of lithium on IL-2 production showed some differences from that of tetradecanoylphorbol acetate (TPA) in the following aspects: (i) TPA could reverse the inhibitory effect of anti-CD2 monoclonal antibody on IL-2 production, whereas lithium could not; and (ii) lithium was unable to synergistically induce IL-2 production with anti-CD3 monoclonal antibody as TPA did. The effect of lithium on IL-2 production was in the early phase of lymphocyte activation. The addition of cholera toxin or theophylline to phytohemagglutinin-stimulated PBMC culture suppressed IL-2 production. However, IL-2 production could be restored by lithium. There was a corresponding increase in cAMP in cholera toxin or theophylline-treated PBMC, which could be reversed by lithium. Therefore, lithium restores IL-2 production via a decrease in cAMP.
Wu
Y Y
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Department of Immunology, Chinese Academy of Medical Sciences, Beijing.
Yang
X H
XH
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000494:pharmacology / Q000502:physiology; D018801:CD2 Antigens; D017252:CD3 Complex; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006801:Humans; D006922:Hylobates; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis*; D008094:Lithium / Q000494:pharmacology*; D011948:Receptors, Antigen, T-Cell / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000502:physiology; D012643:Selenium / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D017252:CD3 Complex; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic; D002772:Cholera Toxin; D008094:Lithium; D013806:Theophylline; D000242:Cyclic AMP; D012643:Selenium; D015032:Zinc; D013755:Tetradecanoylphorbol Acetate