Purine salvage and metabolism in Babesia bovis.
Studies of the incorporation of radio-labelled purine precursors into the erythrocytic forms of Babesia bovis under tissue-culture conditions have confirmed the presence in the parasite of enzymatic activities responsible for the salvage of preformed purines. The results also revealed that the parasite was capable of a variety of nucleotide interconversions, such that exogenous hypoxanthine and adenosine were incorporated into both adenine and guanine nucleotides followed by the incorporation of these nucleotides into the adenine and guanine moieties of RNA and DNA. No evidence was found for salvage of preformed pyrimidines. Evidence was also obtained for the insertion of a parasite-specific nucleoside/nucleobase transporter into the membrane of the bovine (host) red cell. Thus, whereas normal (non-parasitised) bovine red cells are essentially incapable of transporting nucleosides across their membranes, the invasion of these cells by B. bovis introduces a transporter that can be inhibited by classic nucleoside transport inhibitors.
Matias
C
C
School of Biochemistry, University of New South Wales, Kensington, Australia.
Nott
S E
SE
Bagnara
A S
AS
O'Sullivan
W J
WJ
Gero
A M
AM
D000241:Adenosine / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001403:Babesia / Q000378:metabolism*; D001692:Biological Transport; D002417:Cattle; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004912:Erythrocytes / Q000469:parasitology; D006160:Guanosine Triphosphate / Q000378:metabolism; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D007291:Inosine Monophosphate / Q000378:metabolism; D009705:Nucleosides / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D007042:Hypoxanthines; D009705:Nucleosides; D011687:Purines; D007291:Inosine Monophosphate; D019271:Hypoxanthine; D012313:RNA; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D004247:DNA; D000241:Adenosine; D013936:Thymidine