Regeneration of enzymatic activity after sodium dodecyl sulfate/polyacrylamide gel electrophoresis and zinc acetate staining: the example of inositol 1,4,5-trisphosphate 5-phosphatase.
Regeneration of enzyme activity after sodium dodecyl sulfate-gel electrophoresis was investigated with a purified inositol 1,4,5-trisphosphate 5-phosphatase. In order to avoid silver or Coomassie blue staining, we have used zinc acetate. This staining procedure was sensitive, rapid, and reversible provided that zinc cations are chelated and activity is extracted after diffusion out of the gel. The method allows some gel lane staining and identification of the enzyme based on catalytic activity.
Delvaux
A
A
Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Belgium.
Lemos
M
M
Moreau
C
C
Erneux
C
C
D000085:Acetates; D019342:Acetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D011755:Pyrophosphatases / Q000032:analysis / Q000378:metabolism*; D012834:Silver; D013194:Staining and Labeling; D000097630:Inosine Triphosphatase
D000085:Acetates; D019342:Acetic Acid; D011755:Pyrophosphatases; D012834:Silver; D000097630:Inosine Triphosphatase