Antigenic epitope in Pseudomonas aeruginosa lipopolysaccharide immunologically cross-reactive with Escherichia coli O26 lipopolysaccharide.
The human monoclonal antibody MH-4H7 recognizes the lipopolysaccharide outer core region of some Pseudomonas aeruginosa strains and in of some Pseudomonas aeruginosa strains and in particular strongly binds to strains of Lányi serotype 04. In this paper, we report that this monoclonal antibody also reacts with Escherichia coli O26 LPS. However, our results suggest that the previous reported immunological cross reaction between P. aeruginosa 04 and E. coli O26 strains (which was observed by using antisera against heat-stable antigens) is not due to the similarity of the O-polysaccharides.
Laboratory of Biotechnology, Takarazuka Research Center, Sumitomo Chemical Co. Ltd., Hyogo, Japan.
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Yokota
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Kaya
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Noguchi
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology*; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology*; D019081:O Antigens; D011550:Pseudomonas aeruginosa / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D002241:Carbohydrates; D000939:Epitopes; D008070:Lipopolysaccharides; D019081:O Antigens