Phosphoinositides and synaptic function in NG108-15 neuroblastoma x glioma hybrid cells.
1. The second-messengers system of bradykinin (BK) receptors was examined in NG108-15 neuroblastoma x glioma hybrid cells. 2. An application of BK induced an immediate outward (K+) current and acetylcholine (ACh) release, which are generated through inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ ions. 3. Application of phorbol dibutyrate (a protein kinase C activator) produced a voltage-dependent inward current and inhibited another K+ (M)-current. 4. A similar current response has been produced by ACh in NG108-15 cells transfected with rodent muscarinic ACh receptor I and III subtype genes. 5. These results suggest a dual and time-dependent role for these two intracellular messengers in the control of neuronal signalling by BK and ACh.
Department of Biophysics, Kanazawa University School of Medicine, Japan.
H
H
Higashida
S
S
Yokoyama
N
N
Hoshi
Y
Y
Myojo
T
T
Kawamura
Y
Y
Ito
M
M
Hashii
J
J
Sagara
K
K
Furuya
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology; D005910:Glioma / Q000503:physiopathology*; D006822:Hybrid Cells / Q000502:physiology*; D009447:Neuroblastoma / Q000503:physiopathology*; D010716:Phosphatidylinositols / Q000502:physiology*; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology; D015290:Second Messenger Systems; D013569:Synapses / Q000502:physiology*; D014407:Tumor Cells, Cultured
D010716:Phosphatidylinositols; D015221:Potassium Channels; D000109:Acetylcholine; D001920:Bradykinin