Simultaneous determination of DOPAC, MHPG, 5-HIAA and HVA in CSF by direct injection on a liquid chromatograph with coulometric detection.
We describe a direct analysis for simultaneous determination of 3,4-dihydroxyphenylacetic acid (DOPAC), 3-methoxy-4-hydroxyphenylglycol (MHPG), 5-hydroxyindoleacetic acid (5-HIAA), and 4-hydroxy-3-methoxyphenylacetic acid (HVA). After ultrafiltration the samples are applied directly to a high-performance liquid chromatograph with coulometric detection. The appropriate choice of the potentials of the three-coulometric-electrode system eliminates many possible interfering substances. One chromatographic run requires less than 15 min. By this analytical system the lowest amount of DOPAC, MHPG, 5-HIAA and HVA detectable was 0.16, 0.18, 0.90, and 1.48 ng/ml respectively. Coefficient of variation was less than 5% for "within-run" precision and less than 10% for "between-run" precision.
Clinical Chemistry and Neurochemistry Laboratory, IRCCS C. Mondino, University of Pavia, Italy.
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D015102:3,4-Dihydroxyphenylacetic Acid / Q000134:cerebrospinal fluid*; D002853:Chromatography, Liquid / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid*; D008734:Methoxyhydroxyphenylglycol / Q000134:cerebrospinal fluid*; D012016:Reference Values; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation
D015102:3,4-Dihydroxyphenylacetic Acid; D008734:Methoxyhydroxyphenylglycol; D006897:Hydroxyindoleacetic Acid; D006719:Homovanillic Acid