Production of granulopoiesis-stimulating and -inhibiting activities by T cells associated with malignant cells in lymphomas.
The possible role of T lymphocytes in the formation of granulomatous reactions seen in certain malignant lymphoid tumours was investigated by measuring the granulopoietic colony-stimulating activity (CSA) and granulopoietic-inhibiting activity (IA) produced by stimulated T-lymphocytes isolated from peripheral blood, spleen and lymph nodes of patients and normal subjects. Lymph-node T-cells from patients with benign lymphoid hyperplasia, B-cell non-Hodgkin's lymphoma (B-NHL), and non-granulomatous Hodgkin's disease (HD) showed no CSA, but the cells produced IA of 40 +/- 23%, 40 +/- 24% and 50.5 +/- 22.5% respectively. The corresponding cells from patients with HD accompanied by granulomatous reactions produced CSA of 6.85 +/- 6.5 u/microliters and IA of 23.5 +/- 21%. The presence of a granulomatous reaction in malignant lymphoma was correlated with the stimulation of granulopoiesis in vitro by T lymphocytes associated with malignant cells. A correlation was demonstrated between neutrophilic and eosinophilic colonies obtained in vitro under the influence of CSA-producing T cells isolated from malignant lymphomas and the neutrophils and eosinophils present in the granuloma. These results showed that tumour-infiltrating T cells play a role in the presence of granulomatous reactions seen in lymphomas. Peripheral-blood T cells from healthy subjects, and from patients with B-NHL, or with HD unaccompanied by granulocytic reactions produced CSAs of, respectively, 5 +/- 0.5 u/microliter, 4.8 +/- 2.2 u/microliters and 5.3 +/- 0.4 u/microliters, and IAs of 45 +/- 18%. 50 +/- 5.5% and 50.5 +/- 7% respectively. The corresponding values for HD patients with granulocytic reactions were CSA. 17 +/- 15.5 u/microliters, and IA, 9.5 +/- 9%. No correlation was demonstrated between neutrophilic colonies obtained in vitro under the influence of HD blood T cells and neutrophils present in blood. Only one correlation was found: between the percentage of eosinophilic colonies and the number of blood eosinophils. HD blood T cells did not seem to explain completely granulocytic reactions seen in blood.
Piccinni
M P
MP
Service d'Hématologie, Hôpital A. Michallon, Grenoble, France.
Jacob
M C
MC
Bonnefoix
T
T
Sotto
M F
MF
Pegourie
B
B
Couderc
P
P
Sotto
J J
JJ
D001402:B-Lymphocytes / Q000276:immunology; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D016179:Granulocyte Colony-Stimulating Factor; D006689:Hodgkin Disease / Q000276:immunology; D006801:Humans; D006965:Hyperplasia / Q000276:immunology; D008198:Lymph Nodes / Q000473:pathology; D008223:Lymphoma / Q000097:blood / Q000276:immunology*; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology; D014176:Protein Biosynthesis; D011506:Proteins; D013601:T-Lymphocytes / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D011506:Proteins; D016179:Granulocyte Colony-Stimulating Factor; C016313:colony inhibiting factor