Phorbol ester induces morphological changes and acidic fibroblast growth factor RNA expression in the RD/TE 671 cell line.
The TE 671 medulloblastoma cell line was induced by phorbol-12-myristate-13-acetate (PMA) to resemble neuronal cells by both morphological and immunohistochemical criteria. Five days following PMA treatment at a concentration of 10 ng/ml, the cells acquired long cellular processes and cell growth was markedly inhibited. Immunohistochemically, PMA-treated cells stained intensely for neuron-specific enolase and were positive for neurofilaments. Northern blot analysis revealed that PMA induced the transcript for acidic fibroblast growth factor. Our data suggest that the TE 671 cell may be useful not only in the study of acidic fibroblast growth factor regulation but also as a model for embryonic tumor cell differentiation.
Hooper
W C
WC
Division of Immunologic, Oncologic and Hematologic Diseases, Centers for Disease Control, US Department of Health and Human Services, Atlanta, Ga.
Zaki
S R
SR
Pruckler
J
J
Garcia
J
J
Evatt
B L
BL
D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D016220:Fibroblast Growth Factor 1 / Q000096:biosynthesis*; D006801:Humans; D008527:Medulloblastoma / Q000378:metabolism / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012208:Rhabdomyosarcoma / Q000378:metabolism / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D016220:Fibroblast Growth Factor 1; D012313:RNA; D010751:Phosphopyruvate Hydratase; D013755:Tetradecanoylphorbol Acetate