A combined AgNOR-Feulgen staining technique.
We describe a new staining technique (H-Ag-S) which allows observation and counting of active nucleolus organizer regions (NORs) and evaluation of the amount of DNA in the same cell nucleus. The procedure consists of combining a modified AgNOR staining method with the Feulgen reaction. A sequential procedure is proposed, based on the determination of optimal staining conditions. The technique, which was designed to allow studies of correlations between the transcriptional activity of rDNA genes and the cell ploidy, was primarily developed for rat liver smears. It should be applicable to most biological preparations, but the optimal conditions might be variable.
Foucrier
J
J
Laboratoire de Biologie du Développement et de la Différenciation, U.F.R., Santé Médecine et Biologie Humaine, Bobigny, France.
Rigaut
J P
JP
Pechinot
D
D
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D004396:Coloring Agents / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000235:genetics; D006651:Histocytochemistry / Q000379:methods*; D008099:Liver / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000378:metabolism / Q000648:ultrastructure*; D011003:Ploidies; D051381:Rats; D011919:Rats, Inbred Strains; D012394:Rosaniline Dyes; D012834:Silver / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D012834:Silver; D004247:DNA