Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents.
The inhibition of voltage-dependent Ca2+ channels in secretory cells by plasma membrane receptors is mediated by pertussis toxin-sensitive G proteins. Multiple forms of G proteins have been described, differing principally in their alpha subunits, but it has not been possible to establish which G-protein subtype mediates inhibition by a specific receptor. By intranuclear injection of antisense oligonucleotides into rat pituitary GH3 cells, the essential role of the Go-type G proteins in Ca(2+)-channel inhibition is established: the subtypes Go1 and Go2 mediate inhibition through the muscarinic and somatostatin receptors, respectively.
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany.
C
C
Kleuss
J
J
Hescheler
C
C
Ewel
W
W
Rosenthal
G
G
Schultz
B
B
Wittig
D000818:Animals; D001483:Base Sequence; D015220:Calcium Channels / Q000502:physiology*; D002217:Carbachol / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D016373:DNA, Antisense / Q000235:genetics; D004553:Electric Conductivity; D004594:Electrophysiology; D019204:GTP-Binding Proteins / Q000235:genetics / Q000502:physiology*; D008845:Microinjections; D008969:Molecular Sequence Data; D037342:Pertussis Toxin; D010902:Pituitary Gland / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011976:Receptors, Muscarinic / Q000502:physiology*; D017981:Receptors, Neurotransmitter / Q000502:physiology*; D017481:Receptors, Somatostatin; D015398:Signal Transduction; D013004:Somatostatin / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D015220:Calcium Channels; D016373:DNA, Antisense; D012333:RNA, Messenger; D011976:Receptors, Muscarinic; D017981:Receptors, Neurotransmitter; D017481:Receptors, Somatostatin; D010566:Virulence Factors, Bordetella; D013004:Somatostatin; D002217:Carbachol; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins