Investigation of prostaglandins in the culture supernatant of mononuclear cells and its influence on platelet aggregation.
The prostaglandins can be synthesized by many cells types. Cells of the immune system also metabolise arachidonic acid to prostaglandins. However, the specific class of immunocompetent cells that synthesize prostaglandins as well as the spectrum of arachidonic acid metabolides produced by these cells is not firmly established. The aim of our study was to investigate the behaviour of prostaglandins in the culture supernatant of mononuclear cells and the influence of this supernatant on platelet aggregation. Blood cells were separated from peripheral blood according to a modification of the procedure of Böyum. The level of prostaglandins was determined by means of radioimmunoassay kits. The PGF2 alpha concentrations were significantly higher in the culture supernatant (F) in comparison with supernatant (K) and (O). The supernatant of lymphocytes culture does not influence platelet aggregation. The highest concentrations of PGE1 and PGF2 alpha were noted at the 12 and 24 hours of the monocyte cultivation, however the lowest at the 36 and 48 hours. The supernatant obtained from monocyte cultivation at the 36 hours exert an independent effect on platelets aggregation, whereas at 24 and 48 hours it plays a role in platelet aggregation. The presented results may indicate the influence of monocytes on AA metabolism and platelet function.
Department of Haematology, Medical School, Białystok, Poland.
J
J
Giedrojć
M
M
Bielawiec
A
A
Bodzenta-Lukaszyk
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D000527:Alprostadil / Q000097:blood; D002478:Cells, Cultured; D003470:Culture Media; D015237:Dinoprost / Q000097:blood; D004731:Endotoxins / Q000494:pharmacology; D004926:Escherichia coli; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D010974:Platelet Aggregation; D011453:Prostaglandins / Q000097:blood*; D012016:Reference Values
D003470:Culture Media; D004731:Endotoxins; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D011453:Prostaglandins; D000244:Adenosine Diphosphate; D015237:Dinoprost; D000527:Alprostadil