Proliferating cell nuclear antigen and Ki-67 immunohistochemistry in brain tumors: a comparative study.
Proliferating cell nuclear antigen (PCNA) is a 36-kDa DNA polymerase-delta auxiliary protein which accumulates in the nucleus during S phase of the cell cycle. Immunohistochemical labeling indices (LI) of PCNA and Ki-67 were compared using an avidin-biotin complex method on frozen sections of 27 nervous system tumors. 3 normal cerebral cortices, and 3 peripheral nerves. In glial tumors, PCNA and Ki-67 LI increased with increasing tumor grade (Daumas-Duport system). In 5 low-grade glial tumors, PCNA and Ki-67 LI were less than or equal to 1%, except for one optic nerve glioma (Ki-67 LI = 6%). In 7 grade 3 astrocytomas and 1 mixed glioma, PCNA LI were less than or equal to 1-1.5%, while Ki-67 LI were 2%-10%. In 7 grade 4 astrocytomas and 1 metastatic carcinoma, PCNA LI ranged from 6%-15% while Ki-67 LI ranged from 17%-30%. In 5 of 6 schwannomas, focally high PCNA LI (4%-65%) were noted, despite low LI with Ki-67 (less than or equal to 1.6%). Scattered normal schwann cell nuclei also stained with PCNA, but normal cerebral cortex did not.(ABSTRACT TRUNCATED AT 250 WORDS)
Louis
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Department of Pathology (Neuropathology), Massachusetts General Hospital, Boston 02114.
Edgerton
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Thor
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Hedley-Whyte
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D001932:Brain Neoplasms / Q000276:immunology*; D002467:Cell Nucleus / Q000276:immunology; D002540:Cerebral Cortex / Q000276:immunology; D005910:Glioma / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D009687:Nuclear Proteins / Q000276:immunology*; D010525:Peripheral Nerves / Q000276:immunology; D018809:Proliferating Cell Nuclear Antigen; D014407:Tumor Cells, Cultured / Q000276:immunology
D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen