Monoclonal antibodies against sensory neuron specific antigens define the extent of neuronal abnormality in the mf mutant rat.
The mutant rat mutilated foot (mf) is affected by a sensory neuropathy which does not involve the parts of the body innervated by the thoracic cord. The possibility that sensory cells subserving clinically normal regions may be functionally spared by the mutation has been investigated by studying the expression of cell surface oligosaccharides by dorsal root ganglia (DRG) and their central processes in the spinal cord. The study included 3 lactoseries epitopes (TC6, LD2 and LA4) and the globoseries epitope SSEA3. The results show that at cervical and lumbar levels in mf rats there are reduced numbers of DRG cells reacting with the various antibodies and less immunostaining in the dorsal horns. The unexpected finding that thoracic ganglia and cord share similar appearances suggests that, in spite of being normal in number and able to produce normal amounts of substance P, thoracic DRG cells in mf rats take part in the mutation as shown by their inability to produce normal amounts of oligosaccharides and to transport them to the axon terminals.
Scaravilli
F
F
Department of Neuropathology, Institute of Neurology, Queen Square, London, UK.
Jessell
T M
TM
Dodd
J
J
Chimelli
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis / Q000276:immunology*; D002452:Cell Count; D000939:Epitopes; D005727:Ganglia, Spinal / Q000276:immunology / Q000473:pathology; D009154:Mutation; D009422:Nervous System Diseases / Q000235:genetics / Q000276:immunology; D009475:Neurons, Afferent / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D012677:Sensation / Q000502:physiology*; D013116:Spinal Cord / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes