The effects of salt-loading and dehydration on atrial natriuretic peptide (ANP) gene expression.
1. Effects of sodium loading and dehydration on ANP gene expression were investigated in rats. 2. ANP-mRNA was determined using Northern blot and dot blot hybridization technique with alpha-32P-labeled r-preproANP-cDNA probe. Salt loading increased the ANP-mRNA content in atria. Correlation with ANP-mRNA content and the plasma sodium concentration was established. 3. Deprivation of water for 2 and 4 days increased ANP-mRNA 2.1- and 1.6-fold, respectively. 4. These results demonstrated that water-salt balance affects the ANP-gene expression.
Hong
M
M
Department of Biochemistry and Molecular Biology, Norman Bethune Medical University, Chang-Chun, Jilin Province, People's Republic of China.
Jin
Y
Y
Mai
Y Q
YQ
Han
K K
KK
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D015152:Blotting, Northern; D003681:Dehydration; D005260:Female; D005786:Gene Expression Regulation; D012313:RNA / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology*
D012313:RNA; D009320:Atrial Natriuretic Factor; D012964:Sodium