An anticarbohydrate monoclonal antibody inhibits cell-cell adhesion in many species of Dictyostelium but not of Polysphondylium.
The anticarbohydrate monoclonal antibody d-41 inhibits the adhesion of aggregating cells, as measured by an in vitro assay, in every species of Dictyostelium tested but in none of the species from the genus Polysphondylium. Although d-41 binds significantly to the surface of cells from both genera, the ability to inhibit adhesion correlates with the binding of the antibody to a few, mostly developmentally regulated, membrane-associated proteins in each of the species affected. Previous work in D. discoideum and D. purpureum have shown that the major d-41-b binding proteins from these species at this time in development are directly involved in the adhesion process. Therefore, the presence of the epitope on these proteins in the other species of Dictyostelium implicates them in the adhesion mechanism. The function of the carbohydrates containing the epitope is yet to be determined.
Department of Psychiatry, Veterans Affairs Medical Center, San Diego, California 92161.
W R
WR
Springer
J A
JA
Ahern
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002241:Carbohydrates / Q000276:immunology* / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D004023:Dictyostelium / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D000939:Epitopes / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology* / Q000502:physiology; D009235:Myxomycetes / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D000939:Epitopes; D008565:Membrane Proteins