C-erb B-2 amplification in cystic renal disease.
Gene amplification (overexpression) of c-erb B-2 was tested in a variety of cystic renal diseases, renal cell neoplasms (adenomas and carcinomas) and end stage kidneys without cysts. C-erb B-2 encodes a receptor-like protein that shares homology with, but is distinct from the epidermal growth factor (EGF) receptor. A monoclonal antibody that immunoprecipitates a protein of approximately 185 kD from a lysate of NIH/3T3 cells transfected with the c-erb B-2 gene was utilized for testing. Simple renal cysts, cystic renal dysplasia, autosomal recessive polycystic kidney disease (ARPKD), and non-cystic, essentially normal kidneys failed to show c-erb B-2 overexpression. In contrast, autosomal-dominant polycystic kidney disease (ADPKD), acquired (dialysis-associated) cystic disease (ACD), non-cystic end stage kidneys and renal cell neoplasms revealed overexpression of c-erb B-2 with some frequency (40% or more of cases tested). Three cystic disorders revealing c-erb B-2 overexpression also showed platelet-derived growth factors (PDGFs) expression in similar locations (cyst lining and adjacent tubules). Other growth factors [insulin-like growth factor (IGF-I), fibroblast growth factor (FGF) and beta transforming growth factor (TGF beta)] were not noted to be overexpressed in either c-erb B-2 positive or negative cystic diseases. C-erb B-2 may be a marker related to the proliferative/growth capabilities of selected cystic diseases, including potential for associated genesis of benign and malignant renal cell tumors.
Herrera
G A
GA
Department of Pathology, University of Mississippi Medical Center, Jackson.
D000236:Adenoma / Q000235:genetics; D000911:Antibodies, Monoclonal / Q000276:immunology; D002292:Carcinoma, Renal Cell / Q000235:genetics; D004848:Epithelium / Q000737:chemistry; D005784:Gene Amplification; D006133:Growth Substances / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000737:chemistry; D052177:Kidney Diseases, Cystic / Q000235:genetics*; D007676:Kidney Failure, Chronic / Q000235:genetics; D007680:Kidney Neoplasms / Q000235:genetics; D007690:Polycystic Kidney Diseases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D011519:Proto-Oncogenes; D018719:Erb-b2 Receptor Tyrosine Kinases
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases