Karyometric marker features in fine needle aspirates of microinvasive follicular carcinoma of the thyroid.
Karyometric measurements were performed on fine needle aspirates of clearly identifiable tumor areas and adjacent normal-appearing areas in the surgical specimens from ten patients with microinvasive follicular carcinoma of the thyroid. Similar measurements were performed on aspirates from nine patients free of thyroid disease (controls). A total of 95 karyometric features were evaluated for each nucleus. As compared with the control nuclei, the normal-appearing nuclei showed a 6% increase in total nuclear optical density (OD) while the tumor nuclei showed a 14% increase. Analysis of variance indicated a significant difference between the normal-appearing nuclei and the control nuclei, with most of the difference due to the differences of tissue origin. Discriminant analysis selected nine features that produced a statistically highly significant separation of tumor nuclei from control nuclei. A similar analysis selected five features that produced a statistically highly significant discrimination of normal-appearing nuclei from control nuclei; the validity of those karyometric features as markers of malignancy in normal-appearing nuclei from tissues adjacent to microinvasive follicular carcinomas of the thyroid was demonstrated by analysis in further training and test sets of nuclei.
Department of Obstetrics and Gynecology, University of Chicago, IL 60637.
M
M
Bibbo
P H
PH
Bartels
M
M
Salguero
H E
HE
Dytch
E
E
Lerma-Puertas
H
H
Galera-Davidson
D000230:Adenocarcinoma / Q000032:analysis / Q000648:ultrastructure*; D001707:Biopsy, Needle; D002467:Cell Nucleus / Q000032:analysis*; D004396:Coloring Agents; D006801:Humans; D007620:Karyometry; D011003:Ploidies; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D013964:Thyroid Neoplasms / Q000032:analysis / Q000648:ultrastructure*
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes