Aggregation of influenza virus haemagglutinin in the presence of detergents.
In the haemagglutinin (HA) detergent mixtures coexist various protein complexes. Using 125I-HA tracer, gel chromatography, and centrifugation techniques we found protein aggregates comprising up to eight HA trimers. Formation of these structures seemed to be a function of the protein storage medium only. By contrast, special properties of the detergent as well as physicochemical conditions during the protein/detergent interaction had nearly no influence.
Central Institute of Isotope and Radiation Research, Berlin, Germany.
R
R
Stahn
H
H
Schäfer
J
J
Malur
A
A
Ladhoff
D000818:Animals; D002499:Centrifugation, Density Gradient; D002645:Chickens; D002845:Chromatography; D003902:Detergents / Q000737:chemistry*; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure; D009975:Orthomyxoviridae / Q000737:chemistry*; D011487:Protein Conformation
D003902:Detergents; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral