Immunocytochemical localization of human growth hormone- and prolactin-like antigenic determinants in the insects, Locusta migratoria and Sarcophaga bullata.
1. By use of the peroxidase-antiperoxidase immunocytochemical method, substances immunoreactive to antisera directed against human growth hormone (hGH) and prolactin (hPrl) were localized in the nervous system of larval and adult Locusta migratoria and of adult Sarcophaga bullata belonging to different age groups. 2. No major differences in the distribution of cerebral immunoreactive materials were observed between males and females or between juvenile and adult insects. 3. Differential immuno-labeling of alternating tissue sections demonstrated that materials resembling hGH or hPrl are present in distinct neurons in the locust, whereas neurons immunoreactive to both antisera were detected in the fleshfly (Sarcophaga).
Zoological Institute of the University, Leuven, Belgium.
K
K
Swinnen
J V
JV
Broeck
P
P
Verhaert
A
A
De Loof
D000818:Animals; D002490:Central Nervous System / Q000276:immunology; D004175:Diptera / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D006110:Grasshoppers / Q000276:immunology*; D013006:Growth Hormone / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D011388:Prolactin / Q000276:immunology*
D000939:Epitopes; D007106:Immune Sera; D011388:Prolactin; D013006:Growth Hormone