Restriction fragment length polymorphism of the caprine beta-globin gene cluster associated with the Hb beta-globin variants in Norwegian dairy goats.
Restriction fragment length polymorphism (RFLP) analysis was used to characterize the beta-globin cluster in Norwegian dairy goats. In 122 individuals, different RFLP patterns were detected after BglII and PstI digestion and hybridization with a caprine beta-globin probe. The location of the polymorphic BglII and PstI sites were determined. The different RFLPs were in linkage disequilibrium with each other and with the beta-globin locus as studied at the protein level. A preferential association between certain RFLP haplotypes and beta-globin variants was observed. Polymorphic DNA fragments in the epsilon-globin genes were detected after MspI digestion and hybridization with a caprine epsilon-probe, and eight different band patterns were observed. Correlation between different MspI haplotypes and the beta-globin variants was determined.
Norwegian College of Veterinary Medicine, Department of Animal Genetics, National Veterinary Institute, Oslo.
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Bergersen
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Braend
D000483:Alleles; D000818:Animals; D005075:Biological Evolution; D015139:Blotting, Southern; D003433:Crosses, Genetic; D003612:Dairying / Q000145:classification; D014644:Genetic Variation / Q000235:genetics; D005914:Globins / Q000235:genetics*; D006041:Goats / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D005810:Multigene Family / Q000235:genetics; D009664:Norway; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D005914:Globins