Transforming growth factor-beta and epidermal growth factor modulate basal and interleukin-6-induced amino acid uptake and acute phase protein synthesis in cultured rat hepatocytes.
Rat hepatocytes cultured for 2 days with interleukin-6 show increased synthesis of acute phase proteins and enhanced accumulation of 14C-labelled alpha-aminoisobutyric acid. Transforming growth factor-beta 1 (0.1-10 ng/ml) inhibits whereas epidermal growth factor (1-100 ng/ml) enhances both basal and interleukin-6-induced amino acid uptake by rat hepatocytes with only a slight alteration of acute phase protein synthesis.
Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.
J
J
Bereta
K
K
Szuba
W
W
Fiers
J
J
Gauldie
A
A
Koj
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D015291:Transforming Growth Factors / Q000494:pharmacology*
D000209:Acute-Phase Proteins; D000596:Amino Acids; D015850:Interleukin-6; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D004247:DNA