The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors.
Spi-1 is a putative proto-oncogene involved in murine virus-induced acute erythroleukemias. We report here the identification of the human homologue of Spi-1 and its expression in normal and tumorigenic human tissues. Characterization of cDNA clones revealed that the human Spi-1 gene encodes a 216 amino acids protein showing 85% identity with the murine counterpart. By sequencing genomic clones, five exons were identified. To investigate the possible role of Spi-1 gene in human cancers, we studied its expression in a panel of human tumors by Northern blot analysis. Spi-1 expression was detected in all the tumors examined. There was no noticeable evidence of messenger RNA alteration as compared to normal tissues.
Ray
D
D
INSERM U.248, Faculté de Médecine Lariboisière-Saint-Louis, Paris, France.
Culine
S
S
Tavitain
A
A
Moreau-Gachelin
F
F
D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000032:analysis; D003001:Cloning, Molecular; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D006801:Humans; D007438:Introns; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D012313:RNA / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1
D015342:DNA Probes; D004273:DNA, Neoplasm; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012313:RNA; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C000715470:MAS1 protein, human