Quantification of haemoglobin binding of 4,4'-methylenebis(2-chloroaniline) (MOCA) in rats.
4,4'-Methylenebis(2-chloroaniline) (MOCA) is used as a curing agent in the production of polyurethane. MOCA is carcinogenic in experimental animals. Haemoglobin adducts have been proposed as dosimeters of aromatic amines for biological monitoring. A quantitative method to determine the adduct has now been worked out in female Wistar rats dosed per os with 3.82, 14.2 and 16.2 mumol/kg 14C-ring labeled MOCA or 0.25 and 0.50 mmol/kg unlabeled MOCA. MOCA bound in decreasing amounts to DNA, RNA and proteins of the lung, liver and kidney. Fractions of 0.19% and 0.026% of the dose were bound to the blood proteins haemoglobin and albumin, respectively. MOCA released by hydrolysis from haemoglobin was determined by HPLC with electrochemical detection or by GC-MS. Albumin did not form any hydrolysable adducts with MOCA.
Institut für Pharmakologie und Toxikologie, Universität Würzburg, Federal Republic of Germany.
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D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000378:metabolism*; D006868:Hydrolysis; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008753:Methylenebis(chloroaniline) / Q000378:metabolism*; D011402:Pronase; D011485:Protein Binding; D012313:RNA / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000032:analysis / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D001798:Blood Proteins; D006454:Hemoglobins; D012709:Serum Albumin; D008753:Methylenebis(chloroaniline); D012313:RNA; D004247:DNA; D011402:Pronase