Detection, using antibodies, of pharmacologically active sites, apart from the catalytic site, on venom phospholipase A2.
Polyclonal antibodies to a purified neurotoxic phospholipase A2 VRV PL-V from Vipera russelli venom were raised in rabbits. The gamma globulin fraction from the serum of the rabbits injected with VRV PL-V-toxoid (anti VRV PL-V antibodies) is termed anti PL-V Ig. Anti PL-V Ig neutralized the neurotoxic symptoms and lethal effects of neurotoxic PLA2s (VRV PL-V, VRV PL-VI and VRV PL-VIIIa) and neurotoxic symptoms of whole V. russelli venom, without affecting their phospholipase A2 activities. Their edema inducing activity was also unaffected. Anti VRV PL-V-antibodies inhibited the anticoagulant potencies of the neurotoxic PLA2s (VRV PL-V, VRV PL-VI and VRV PL-VIIIa) from V. russelli venom and VRV PL-V-toxoid. The myonecrotic activity of VRV PL-V, VRV PL-VIIIa and whole venom remain unaffected in presence of anti PL-V Ig. The hemoglobinuria induced in rabbits by injection of VRV PL-V-toxoid was inhibited by anti VRV PL-V antibodies. These results indicate the presence of multiple pharmacological sites apart from the catalytic site on V. russelli PLA2 molecules.
Department of Studies in Biochemistry, University of Mysore, Manasagangotri, India.
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Kasturi
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Gowda
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000925:Anticoagulants; D001665:Binding Sites; D002384:Catalysis; D002449:Cell Aggregation; D004487:Edema / Q000139:chemically induced; D005779:Immunodiffusion; D007928:Lethal Dose 50; D051379:Mice; D009336:Necrosis / Q000139:chemically induced; D010740:Phospholipases / Q000032:analysis*; D010741:Phospholipases A / Q000032:analysis* / Q000276:immunology / Q000633:toxicity; D054467:Phospholipases A2; D014757:Viper Venoms / Q000032:analysis / Q000276:immunology* / Q000633:toxicity; D005719:gamma-Globulins / Q000302:isolation & purification
D000906:Antibodies; D000925:Anticoagulants; D014757:Viper Venoms; D005719:gamma-Globulins; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2