In vivo 5-HIAA release from the anterior hypothalamus in the ovariectomized and estradiol treated rat following perfusion with progesterone.
In the present study the frequency and magnitude of the release of 5-Hydroxyindoleacetic Acid (5-HIAA) was measured from the anterior hypothalamus of ovariectomized (OVX) and OVX rats treated with estradiol (E2). Female, Holtzman strain rats were maintained on a photoperiod of 14 H light from 0100 to 1500 H and experiments performed from 0900 to 1700 H. Animals exhibiting four-day estrous cycles (250-300 gms) were OVX (20 days recovery) and a push-pull-cannula (PPC) implanted and stereotaxically aimed at the SCN region in the anterior hypothalamus. Following a 7-10 day recovery push-pull-perfusion (PPP) experiments were performed on either OVX females or on OVX females in which a silastic E2 implant (150 micrograms E2/ml. sesame oil), was placed sc 48 H prior to PPP. In other experiments progesterone (P4) was perfused in a pulsatile manner over the SCN region of the anterior hypothalamus. The overall average 5-HIAA release in the OVX treated rats (548 +/- 358 pg/10 min.) was similar to that in the OVX E2 group (694 +/- 148 pg/10 min). The average period of 5-HIAA release was (48.2 +/- 5.5 min) in the OVX group and (56.0 +/- 9.8 min) in the OVX E2 group. These results indicate that exposure of long term OVX rats (20 days) to E2 has no effect on the release or period of 5-HIAA release from serotonergic terminals concentrated in the SCN of the anterior hypothalamus.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Physiology Eastern Virginia Medical School, Norfolk 23501.
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Meyer
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Holman
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Connel
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McRee
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Jacobs
D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D019855:Ethylene Glycol; D005026:Ethylene Glycols / Q000494:pharmacology; D005260:Female; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism*; D007032:Hypothalamus, Anterior / Q000378:metabolism*; D010052:Ovariectomy; D010477:Perfusion; D014677:Pharmaceutical Vehicles; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D005026:Ethylene Glycols; D014677:Pharmaceutical Vehicles; D011374:Progesterone; D004958:Estradiol; D006897:Hydroxyindoleacetic Acid; D019855:Ethylene Glycol