The effects of ions and hypothalamic factors on the in vitro activity of rainbow trout prolactin cells.
The influence of various ions and of dopamine and somatostatin on the in vitro activity of rainbow trout prolactin (PRL) cells was investigated. There was a positive correlation between medium Ca2+ concentration and both PRL synthesis and release up to 1.8 mM Ca2+, above which no further increase occurred. Even with no Ca2+ in the medium, there was still PRL secretion during the incubation. Replacement of Ca2+ with Ba2+ in the medium did not elevate either total PRL levels or PRL release above that in Ca2 +)-free medium. Neither elevated Mg2+ nor increased medium K+ had any effect on PRL synthesis or release. Dopamine inhibited PRL release but not synthesis, as did the D2 receptor agonist, apomorphine. However, the D2 receptor antagonist, (+)-butaclamol was unable to prevent the action of dopamine on PRL release. Somatostatin inhibited both PRL synthesis and release in normal Ca2+ medium, but release only in reduced Ca2+ medium. Thus, Ca2+, dopamine, and somatostatin may all have roles in regulating prolactin secretion in this fish. In addition, oPRL reduced trout PRL release, indicating a possible negative feedback mechanism for trout PRL secretion.
School of Pure and Applied Biology, University of Wales College of Cardiff, United Kingdom.
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Johnston
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Wigham
D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D001464:Barium / Q000494:pharmacology; D002069:Butaclamol / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002413:Cations, Divalent; D004298:Dopamine / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D011388:Prolactin / Q000096:biosynthesis / Q000378:metabolism*; D012487:Salmonidae / Q000378:metabolism*; D013004:Somatostatin / Q000494:pharmacology*; D014337:Trout / Q000378:metabolism*
D002413:Cations, Divalent; D001464:Barium; D013004:Somatostatin; D011388:Prolactin; D002069:Butaclamol; D008274:Magnesium; D001058:Apomorphine; D011188:Potassium; D002118:Calcium; D004298:Dopamine