A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification.
A simple and rapid method for the extraction, reverse transcription and PCR amplification of RNA from formalin-fixed, paraffin-embedded tissue is described. The procedure can be completed within 24 hours. In a first application of this method we detect human albumin mRNA in liver tissue, demonstrating the feasibility to retrospectively analyze gene expression and RNA viruses in fixed tissue.
von Weizsäcker
F
F
Massachusetts General Hospital, Harvard Medical School, Charlestown.
Labeit
S
S
Koch
H K
HK
Oehlert
W
W
Gerok
W
W
Blum
H E
HE
D000418:Albumins / Q000235:genetics; D001707:Biopsy, Needle; D004247:DNA / Q000096:biosynthesis; D005404:Fixatives; D005557:Formaldehyde; D015870:Gene Expression; D006801:Humans; D007438:Introns; D008099:Liver / Q000737:chemistry*; D010232:Paraffin; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012328:RNA Viruses / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D000418:Albumins; D005404:Fixatives; D012333:RNA, Messenger; D005557:Formaldehyde; D010232:Paraffin; D004247:DNA; D012194:RNA-Directed DNA Polymerase