Markers for human placental trophoblasts in two-dimensional gel electrophoresis.
Primary cultures of trophoblasts established from human term placentae showed high viability and reproducibility. Two-dimensional gel patterns obtained by metabolically labeling the trophoblasts with [35S]-methionine demonstrated that their pattern of gene expression was stable during the 6-d period investigated. Gel analysis demonstrated the keratins 7, 8, 14, 17, 18, and 19. Analysis of the gel pattern confirmed the presence of a small proportion of contaminating fibroblasts and lymphocytes. The gel patterns were compared with that of skin fibroblasts, peripheral lymphocytes, and epithelial cells to identify a group of proteins that are enriched in the trophoblasts and thus may be used as marker for these cells.
Jie
Z
Z
Department of Virus and Cancer, Danish Cancer Society, Aarhus.
Fey
S J
SJ
Hager
H
H
Høllsberg
P
P
Ebbesen
P
P
Larsen
P M
PM
D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007526:Isoelectric Point; D007633:Keratins / Q000378:metabolism; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis*; D014327:Trophoblasts / Q000737:chemistry* / Q000378:metabolism
D011506:Proteins; D007633:Keratins