Phenethyl alcohol disorders phospholipid acyl chains and promotes translocation of the mitochondrial precursor protein apocytochrome c across a lipid bilayer.
The interaction of phenethyl alcohol with model membranes and its effect on translocation of the chemically prepared mitochondrial precursor protein apocytochrome c across a lipid bilayer was studied. Phenethyl alcohol efficiently penetrates into monolayers and causes acyl chain disordering judged from deuterium nuclear magnetic resonance measurements with specific acyl chain-deuterated phospholipids. Translocation of apocytochrome c across a phospholipid bilayer was stimulated on addition of phenethyl alcohol indicating that the efficiency of translocation of this precursor protein is enhanced due to a disorder of the acyl chain region of the bilayer.
Centre for Biomembranes and Lipid Enzymology, State University of Utrecht, The Netherlands.
W
W
Jordi
R
R
Nibbeling
B
B
de Kruijff
D001059:Apoproteins / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D003574:Cytochrome c Group / Q000378:metabolism*; D045304:Cytochromes c; D000431:Ethanol / Q000031:analogs & derivatives*; D008051:Lipid Bilayers / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D001059:Apoproteins; D003574:Cytochrome c Group; D008051:Lipid Bilayers; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010743:Phospholipids; D011498:Protein Precursors; D000431:Ethanol; C042511:1,2-dioleoylphosphatidylserine; C020888:1,2-dielaidoylphosphatidylethanolamine; D045304:Cytochromes c; D014357:Trypsin; C017251:1,2-oleoylphosphatidylcholine; D010626:Phenylethyl Alcohol