N-acetyl-L-galactosaminuronic acid as an epitope common to the O-polysaccharides of Pseudomonas aeruginosa serotype A and H (Homma) recognized by a protective human monoclonal antibody.
We have established a human--mouse heterohybridoma cell line producing a human monoclonal antibody TS-3G2 (IgG gamma 1, K). This monoclonal antibody specifically bound to O-polysaccharides belonging to plural Pseudomonas aeruginosa Homma serotypes, A and H, in contrast to serotype-specific monoclonal antibody which exclusively bound to strains belonging to a single specific serotype. The binding affinity for serotype A strains was higher than that for serotype H strains. Competitive enzyme immunoassay experiments with O-polysaccharide preparations derived from IID 1001, NCTC 8505 (serotype A) and IID 1009 (serotype H) and their derivatives demonstrated that the N-acetyl-L-galactosaminuronic acid residue in O-polysaccharide was essentially involved in the epitope for TS-3G2. Furthermore, a 6-deoxy-hexosamine residue neighboring the reducing terminal of N-acetyl-L-galactosaminuronic acid residues was also concerned with the epitope to some extent. In the experimental infection model of normal mice, the monoclonal antibody TS-3G2 showed a protective activity against both strains of serotype A and H.
Laboratory of Biotechnology, Takarazuka Research Center, Sumitomo Chemical Co., Ltd, Japan.
S
S
Yokota
H
H
Ochi
I
I
Uezumi
H
H
Ohtsuka
K
K
Irie
H
H
Noguchi
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000276:immunology*; D006603:Hexuronic Acids / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011552:Pseudomonas Infections / Q000517:prevention & control; D011550:Pseudomonas aeruginosa / Q000145:classification*; D012703:Serotyping; D014574:Uronic Acids / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006603:Hexuronic Acids; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D014574:Uronic Acids; C065803:N-acetylgalactosaminuronic acid