[The expression of alpha 1-inhibitor 3 gene and some changes of its structure in rat hepatomas].
The expression and structure of alpha 1-inhibitor 3 (alpha 1-I3) gene were investigated in 16 primary rat hepatomas induced by diethylnitrosamine. In most tumor samples (75%, 12/16), the expression of alpha 1-I3 gene manifested markedly diminished or undetectable level of alpha 1-I3 mRNA. Further study indicated that the abnormal expression of alpha 1-I3 gene in the hepatomas examined might be due to, at least in part, the gene hypermethylation, insertion of repeat sequence(s) or base substitutions at the recognition sequences of some restriction endonucleases which caused certain alteration in the gene structure. The significance of alpha 1-I3 as an endogenous antitumor factor in hepatocarcinogenesis was also discussed.
Department of Biochemistry, Chinese Academy of Medical Sciences, Beijing.
Q Z
QZ
Zhao
D000209:Acute-Phase Proteins; D000818:Animals; D004251:DNA Transposable Elements; D004052:Diethylnitrosamine; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics*; D008297:Male; D008745:Methylation; D010634:Phenobarbital; D012150:Polymorphism, Restriction Fragment Length; D011480:Protease Inhibitors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000209:Acute-Phase Proteins; D004251:DNA Transposable Elements; D011480:Protease Inhibitors; C017894:alpha 1-inhibitor 3; D004052:Diethylnitrosamine; D010634:Phenobarbital