Analysis of variation in batches of armadillo-derived Mycobacterium leprae by immunoblotting.
Several batches of cell-free extracts of armadillo-derived Mycobacterium leprae were analyzed by SDS-PAGE and by immunoblotting with monoclonal antibodies. The presence or absence of protease inhibitors had a profound effect on the protein antigens, particularly the 65-kDa antigen. In the absence of protease inhibitors, there were both quantitative and qualitative differences between the different batches of M. leprae extracts.
Ibrahim
M A
MA
Laboratory for Leprosy and Mycobacterial Research, National Institute for Medical Research, Mill Hill, London, U.K.
Lamb
F I
FI
Colston
M J
MJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D001135:Armadillos; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D015151:Immunoblotting; D008970:Molecular Weight; D009166:Mycobacterium leprae / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes