Segregation of afferent projections in the central nervous system of the leech Hirudo medicinalis.
Sensory axons originating in peripheral tissues converge onto each segmental ganglion in the central nervous system (CNS) of the leech, where they segregate into well-defined regions of the synaptic neuropil. Here we report on several aspects of the molecular and anatomical organizations of these afferent projections that bear upon the hypothesis that surface markers are involved in organizing these axons as they grow into the CNS. First, we show that the distribution of some surface markers in the adult is restricted to axons of peripheral origin and is not present on the neighboring axons of central neurons. Second, we demonstrate that the number of afferents increases postembryonically as the leech increases in size, suggesting that at least some of the cues employed by afferent axons to grow to appropriate central targets must be present throughout the life of the animal. We then show, using anterograde axonal tracing and immunohistochemistry, that there is both convergence and divergence of afferent axons into highly specific regions of the neuropil. Lastly, we examine the distribution of surface markers present on different subsets of afferents and show that axons having one type of marker segregate from those having the second type. Our results, considered together with previous observations in this system, provide new clues about the organization of afferent projections in the nervous system of the leech. They also suggest how a relatively small number of molecular markers might mediate fiber-fiber interactions to organize afferent axons as they grow into the CNS.
Department of Biological Sciences, Columbia University, New York, New York 10027.
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Peinado
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Zipser
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Macagno
D000344:Afferent Pathways / Q000033:anatomy & histology / Q000166:cytology / Q000648:ultrastructure; D000375:Aging; D000818:Animals; D001370:Axonal Transport; D006735:Horseradish Peroxidase; D007865:Leeches / Q000033:anatomy & histology*; D008854:Microscopy, Electron; D009420:Nervous System / Q000033:anatomy & histology* / Q000166:cytology / Q000254:growth & development; D009474:Neurons / Q000166:cytology; D009475:Neurons, Afferent / Q000166:cytology / Q000648:ultrastructure
D006735:Horseradish Peroxidase