Hematopoietic stem cell processing and storage.
The techniques to collect, process, and store HSC in anticipation of transplantation are now widely available. Important unresolved issues revolve around the as yet imperfect identification and classification of totipotential progenitors. However, much progress has been and will continue to be made despite this limitation. Research priorities of present and future stem cell processing laboratories should include: 1. Optimization of liquid (nonfrozen) storage techniques. This will permit more complex cell-specific manipulations, such as T-lymphocyte subset selection, isolation of CD34+ populations, treatment in vitro with growth factors, gene transfer experiments, and long-range transport of HSC, to be performed while preserving HSC integrity. 2. A better understanding of the regulation and kinetics of peripheral blood and umbilical cord HSC, to allow optimum collection procedures that do not require marrow harvesting. 3. An intensive study into the optimum conditions of collection, processing, and storage of megakaryocytic progenitors to decrease the long platelet-transfusion dependency of the myeloablated patient. 4. A search for a simple in vitro correlate of engraftment potential of a stem cell preparation. This will greatly improve the quality control functions of the laboratory as well as contribute to better patient selection for transplantation.
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Ciavarella
D000818:Animals; D001772:Blood Cell Count; D001781:Blood Component Removal / Q000295:instrumentation / Q000379:methods; D016913:Blood Component Transfusion / Q000295:instrumentation / Q000379:methods*; D001793:Blood Preservation; D001804:Blood Transfusion, Autologous / Q000295:instrumentation / Q000379:methods*; D001854:Bone Marrow Cells; D016465:Bone Marrow Purging / Q000379:methods; D016026:Bone Marrow Transplantation / Q000379:methods*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D004285:Dogs; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008212:Lymphocyte Depletion