Characterization of monoclonal antibodies directed against distinct conserved epitopes of human immunodeficiency virus type 1 core proteins.
Two mouse hybridoma cell lines secreting antibodies to the Human Immunodeficiency Virus (HIV) p25 major core protein and its precursors p55 and p41, were developed after immunization with the highly cytopathic Zaïrian HIV-1 isolate, NDK. These monoclonal antibodies also react with the gag gene products from HIV-1-BRU prototype and present cross reaction with HIV-2-ROD, and SIV-AGM. They map into topographically distinct areas of p25 and define epitopic regions topographically separated from those recognized by four other anti-p25 mAb suggesting the existence of at least 6 spatially distinct epitopic regions on HIV-1-p25 core protein.
Unité INSERM de Recherches sur les Rétrovirus et Maladies Associées (U.322), Campus Universitaire de Luminy, Marseille, France.
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M
Tatsumi
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C
Devaux
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F
Kourilsky
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JC
Chermann
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D000090266:Chromobox Protein Homolog 5; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D015683:Gene Products, gag / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology* / Q000648:ultrastructure; D015498:HIV-2 / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011498:Protein Precursors / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology / Q000648:ultrastructure; D054301:gag Gene Products, Human Immunodeficiency Virus
D000911:Antibodies, Monoclonal; C000715594:CBX5 protein, human; D000939:Epitopes; D015683:Gene Products, gag; D015488:HIV Antigens; D015700:HIV Envelope Protein gp41; C059827:HIV gag protein p25; D011498:Protein Precursors; D054301:gag Gene Products, Human Immunodeficiency Virus; C059767:p55 gag precursor protein, Human immunodeficiency virus 1; D000090266:Chromobox Protein Homolog 5