Silver staining of collagen fibrils in cartilage.
Direct visualization of individual collagen fibrils by light microscopy in human cartilage was achieved by applying a periodic acid-silver methenamine stain on plastic sections. Collagen fibrils, 100 nm in diameter or thicker, were delineated individually by light microscopy and were easily traced for a length beyond 100 microns. Thinner fibrils not readily visible optically were identified if arranged in compact bundles as occurring in the superficial zone of articular cartilage.
Hwang
W S
WS
Department of Pathology, University of Calgary, Alberta, Canada.
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Saito
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D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D002356:Cartilage / Q000648:ultrastructure*; D002648:Child; D002675:Child, Preschool; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D006821:Hyaluronoglucosaminidase; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D009993:Osmium Tetroxide; D012834:Silver; D013194:Staining and Labeling
D012834:Silver; D003094:Collagen; D006821:Hyaluronoglucosaminidase; D009993:Osmium Tetroxide