Experimental mast cell activation improves connective tissue repair in the perforated rat mesentery.
The participation of mast cells in connective tissue repair was studied using the perforated-rat-mesentery model. Perforation of mesenteric membranes was performed during laparotomy of anesthetized Sprague-Dawley rats. Laparotomy significantly reduced the histamine content of the mesenteric membranes on day 1 postoperatively and perforation as such reduced the histamine content even more on days 1-10. Mast cell activation induced by a single intraperitoneal injection of Compound 48/80 two days prior to operation, significantly improved healing on days 5-7 postoperatively. Daily injections of Compound 48/80 for 5 days prior to operation showed significantly better healing compared to such injections for five days postoperatively. Administration of lupitidine, a long acting histamine H2-receptor antagonist, two times daily starting on the day of 48/80 injection to day 4 after operation did not apparently affect healing. The results indicate that mast cells may be activated during normal wound healing and that a preoperative, pharmacological activation improves healing. Furthermore, histamine does not seem to be of major importance for the beneficial effect of such mast cell activation on connective tissue repair.
Franzén
L
L
Department of Pathology II, University Hospital, Linköping, Sweden.
Ghassemifar
R
R
Malcherek
P
P
D000818:Animals; D003238:Connective Tissue / Q000502:physiology*; D006632:Histamine / Q000378:metabolism; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D008643:Mesentery / Q000293:injuries / Q000378:metabolism / Q000502:physiology*; D011744:Pyrimidinones / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014945:Wound Healing / Q000187:drug effects*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D006635:Histamine H2 Antagonists; D011744:Pyrimidinones; D003189:p-Methoxy-N-methylphenethylamine; C033275:SK&F 93479; D006632:Histamine