Tyrosine phosphorylation is a signal for the trafficking of pp85, an 85-kDa phosphorylated polypeptide associated with phosphatidylinositol kinase activity.
A family of 85/86-kDa (85K/86K) polypeptides closely linked to phosphatidylinositol kinase activity is found in polyoma middle-sized tumor antigen (MTAg)/pp60c-src complexes. MTAg and the 85-kDa phosphoprotein (pp85) could be reassociated in solution, or on blots, after denaturation with SDS. Results from such experiments focus attention on phosphorylation in controlling intracellular sorting and activation of pp85. Tyrosine phosphorylation seems important for recruitment of pp85 from cytosol to membrane. By blotting, pp85 is substantially cytosolic, whereas that recognized by anti-phosphotyrosine antibody is almost exclusively in membranes. Tyrosine phosphorylation also determined association of pp85 with MTAg. Manipulation of MTAg tyrosine phosphorylation, for example, by expressing MTAg using baculovirus vectors in the absence or presence of pp60c-src, dramatically affects reassociation. Finally, tyrosine phosphorylation appears to be involved in release of pp85 from MTAg, since vanadate increased its rate of dissociation.
Department of Biochemistry, Tufts University, Boston, MA 02111.
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Cohen
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Yoakim
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Piwnica-Worms
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Roberts
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Schaffhausen
D019870:1-Phosphatidylinositol 4-Kinase; D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D019000:Phosphotyrosine; D011120:Polyomavirus / Q000235:genetics / Q000276:immunology; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D000952:Antigens, Polyomavirus Transforming; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D019000:Phosphotyrosine; D014443:Tyrosine; D010770:Phosphotransferases; D019870:1-Phosphatidylinositol 4-Kinase