Serologically defined linear epitopes in the E2 envelope glycoprotein of Semliki Forest virus.
A set of 41 overlapping peptides, representing the complete sequence of SFV-E2 protein were synthesized and analyzed in the ELISA test against murine anti-SFV sera. No single peptide was recognized by all antisera. Eight peptides were found to be highly reactive with hyperimmune anti-SFV sera. Six out of the eight peptide sequences coincide with the most hydrophilic regions of SFV-E2. Out of these, four peptides (amino acid positions 16-35, 61-80, 166-185, 286-305) that contain the least number of alphavirus conserved residues were selected. This panel constitutes the minimal number of peptides necessary and sufficient for specific recognition of hyperimmune mouse anti-SFV sera.
Ariel
N
N
Department of Biochemistry, Israel Institute for Biological Research, Ness Ziona.
Lehrer
S
S
Elhanaty
E
E
Sabo
T
T
Brodt
P
P
Lachmi
B
B
Katz
D
D
Levin
R
R
Grosfeld
H
H
Velan
B
B
D000956:Antigens, Viral / Q000138:chemical synthesis / Q000276:immunology*; D001202:Ascitic Fluid / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D012672:Semliki forest virus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000138:chemical synthesis / Q000276:immunology*; D014764:Viral Proteins / Q000138:chemical synthesis / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D010446:Peptide Fragments; D014759:Viral Envelope Proteins; D014764:Viral Proteins