Study of vimentin expression in non-Hodgkin's lymphoma using paraffin sections.
Human non-Hodgkin's lymphomas were studied by means of an avidin-biotin complex immunoperoxidase method using several monoclonal antibodies against the intermediate filament protein, vimentin. The study cases were 61 B-cell lymphomas (including 2 plasmacytomas) and 30 T-cell lymphomas (including 8 cases of mycosis fungoides). Twelve of the 61 B-cell lymphomas were positive for vimentin, and were composed of extrafollicular-center cells such as immunoblastic and plasmacytoid cells. On the other hand, lymphomas of follicular center cell origin were negative for vimentin. All cases of T-cell lymphoma except for 14 (all of 9 AILD-type lymphomas, all of 4 lymphoblastic lymphomas and one diffuse mixed small/large lymphoma) were positive for vimentin. Although vimentin expression appeared to be influenced by various conditions such as the proportion of T- and B-cell subsets, or B-cell proliferation rate, follicular center cells were constantly negative for vimentin.
First Department of Pathology, Chiba University School of Medicine, Japan.
J
J
Tamaru
A
A
Mikata
M
M
Itami
T
T
Takagi
D006652:Histological Techniques; D006801:Humans; D016393:Lymphoma, B-Cell / Q000378:metabolism; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism*; D016399:Lymphoma, T-Cell / Q000378:metabolism; D009182:Mycosis Fungoides / Q000378:metabolism; D010232:Paraffin; D013194:Staining and Labeling; D014746:Vimentin / Q000378:metabolism*
D014746:Vimentin; D010232:Paraffin