Specificity of two subsets of cytotoxic human gamma delta T-cell clones.
Peripheral blood mononuclear cells were enriched for gamma delta T cells by immunomagnetic separation, stimulated with cells from an allogeneic donor, and cloned. T-lymphocyte clones (TLC) of the two major gamma delta T-cell subsets, BB3+ (i.e. V delta 2+) and delta TCS1+ (i.e. V delta 1/(D)/J delta 1), were obtained. In addition, one gamma delta TLC was BB3- delta TCS1-. All of the BB3+ TLC showed strong cytotoxicity against various allogeneic tumor cell lines, such as Daudi and K562. The cytotoxicity against the tumour cell lines was modulated by MoAb against the gamma delta TcR. The BB3+ TLC were not cytotoxic against B-lymphoblastoid cell lines (B-LCL). In contrast, the delta TCS1+ TLC showed much lower cytotoxic activity against the tumour cell lines, but many were strongly cytotoxic against allogeneic B-LCL. Some of the delta TCS1+ TLC demonstrated HLA-specific cytotoxicity, while other delta TCS1+ TLC had a more broad cytolytic activity against B-LCL. Thus, the two major subtypes of gamma delta T cells from this donor, as defined by MoAb BB3 and delta TCS1, were distinct with respect to recognition specificity.
Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
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Qvigstad
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Bosnes
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KE
Lundin
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Thorsby
D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006801:Humans; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta