Ultrastructural and immunocytochemical characterization of cultured cells from rat molar stellate reticulum.
By scanning electron microscopy, the cultured cells were stellate and had numerous filipodia--characteristics of stellate reticulum cells in vivo. By transmission electron microscopy, they contained bundles of intermediate filaments, numerous mitochondria, large electron-dense granules and desmosomes--all features of the stellate reticulum in vivo. Moreover, the stellate reticulum was the only region of the enamel organ in vivo that contained large, electron-dense granules. By immunocytochemistry, the cultured cells contained cytokeratins, confirming their epithelial nature, and stellate reticulum cells in vivo and in vitro did not have an EGF receptor. Thus, these combined ultrastructural and immunocytochemical findings suggest that the cell culture was of stellate reticulum.
Department of Anatomy and Cell Biology, Texas College of Osteopathic Medicine, Fort Worth 76107-2690.
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Wise
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Rudick
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Brun-Zinkernagel
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Fan
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques; D003795:Dental Sac / Q000166:cytology; D004658:Enamel Organ / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D066246:ErbB Receptors / Q000032:analysis; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008854:Microscopy, Electron; D008963:Molar; D051381:Rats; D011919:Rats, Inbred Strains
D007633:Keratins; D066246:ErbB Receptors; D000469:Alkaline Phosphatase