Zonation of total and plasma protein turnover in rat liver: effects of chronic ethanol treatment.
To study the acinar distribution of protein turnover, rats were given a tracer dose of 14C-valine intraperitoneally. At 30 min or 20 hr later, livers were perfused in situ and periportal (pp) or perivenous (pv) cell lysates were collected after digitonin pulse infusion. In control rats the post-label radioactivity of cell lysates showed no zonation. The radioactivity of the pp and pv cell lysates was significantly higher in livers of chronically ethanol-fed rats than in the controls, and the increase was more prominent in the pp region. Furthermore, after chronic ethanol feeding, an enrichment of plasma protein in the pv region was observed. The results support the view that in the control rats liver protein turnover is not zonated. Chronic ethanol feeding may, however, induce such zonation, as evidenced by both a slower rate of protein turnover in the pp cells and preferential pv accumulation of plasma proteins.
Department of Biochemistry, College of Veterinary Medicine, Helsinki, Finland.
A R
AR
Pösö
K E
KE
Penttilä
K O
KO
Lindros
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D004032:Diet; D000431:Ethanol / Q000008:administration & dosage* / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000097:blood; D008099:Liver / Q000098:blood supply / Q000201:enzymology / Q000378:metabolism*; D011169:Portal Vein; D051381:Rats; D011919:Rats, Inbred Strains
D015415:Biomarkers; D001798:Blood Proteins; D000431:Ethanol; D000410:Alanine Transaminase; D005974:Glutamate-Ammonia Ligase