Distinction of deep versus superficial clinical and nonclinical isolates of Trichosporon beigelii by isoenzymes and restriction fragment length polymorphisms of rDNA generated by polymerase chain reaction.
Fifteen clinical and environmental strains of Trichosporon beigelii were analyzed for similarities by using morphological features, biochemical profiles based on carbon compound assimilation and uric acid utilization, isoenzyme electrophoresis, and restriction fragment length polymorphisms of a segment of genes coding for rRNA expanded with the polymerase chain reaction. The findings suggest that strains that cause invasive disease are distinct from the superficial and the nonclinical isolates and that isolates from the skin and mucosae represent a number of different organisms, including some environmental forms. The study shows that T. beigelii is a complex of genetically distinct organisms and that more than one type is found in clinical samples.
Kemker
B J
BJ
Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.
Lehmann
P F
PF
Lee
J W
JW
Walsh
T J
TJ
D001483:Base Sequence; D002374:Catalase / Q000032:analysis; D002874:Chromosome Mapping; D004271:DNA, Fungal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000032:analysis; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics*; D013482:Superoxide Dismutase / Q000032:analysis; D014250:Trichosporon / Q000502:physiology*; D014527:Uric Acid / Q000378:metabolism; D000520:alpha-Glucosidases / Q000032:analysis; D001617:beta-Glucosidase / Q000032:analysis
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D007527:Isoenzymes; D012335:RNA, Ribosomal; D014527:Uric Acid; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D004950:Esterases; D000520:alpha-Glucosidases; D001617:beta-Glucosidase