In vitro characterization of rat thymic macrophages.
Thymic macrophages have been isolated from Wistar rats and maintained in long-term culture. Day 1-isolated thymic macrophages expressed MHC class I and II antigens, Thy-1, CR3, CD4, ED1, ED2, as well as acid phosphatase and non-specific esterase activities. Whereas cultured macrophages were positive for the activation antigen recognized by the mAb OX48, which was not expressed after isolation, MHC class II and Thy-1 expression were down-regulated during the culture, but could be induced with human rIL-2 or with Con A-SCM, which are also inducers of IL-2R, a cell-surface marker not expressed on Day 1 or on cultured macrophages.
Faculty of Biology, Complutense University, Madrid, Spain.
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Navarro
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Ardavin
A M
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Fontecha
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Alvarez
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Zapata
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007376:Interleukin-2 / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D011951:Receptors, Complement / Q000032:analysis; D017463:Receptors, Complement 3b; D011994:Recombinant Proteins / Q000276:immunology; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000276:immunology*; D013997:Time Factors
D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011994:Recombinant Proteins; D018800:Thy-1 Antigens; D003208:Concanavalin A