Bacterial RNA isolation with one hour centrifugation in a table-top ultracentrifuge.
A procedure for the rapid preparation of cesium-chloride purified RNA from E. coli and the cyanobacterium Synechococcus sp. PCC7942 is described. Cells are lysed in modified sucrose, Triton X-100, EDTA, Tris buffer with phenol/chloroform. The cleared lysate is extracted further with phenol/chloroform and RNA is peleted by centrifugation through a 5.7 M CsCl cushion. High quality RNA can be prepared in three hours using this procedure.
Reddy
K J
KJ
Dept. of Biological Sciences, Purdue University, West Lafayette, IN 47907.
Webb
R
R
Sherman
L A
LA
D001312:Aurintricarboxylic Acid; D015152:Blotting, Northern; D002499:Centrifugation, Density Gradient / Q000295:instrumentation / Q000379:methods; D000458:Cyanobacteria / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D012329:RNA, Bacterial / Q000302:isolation & purification*; D014461:Ultracentrifugation / Q000295:instrumentation / Q000379:methods
D012329:RNA, Bacterial; D001312:Aurintricarboxylic Acid