Hydroxyurea as a suppressor of the radioprotective effect of dextran sulfate on bone marrow hemopoietic stem cells in mice.
The changes in hemopoiesis of mice following intraperitoneal administration of dextran sulfate (DS) 24 h before irradiation, as well as the effect of injecting them with hydroxyurea (HU), were analyzed. DS increased the proliferation activity and number of hemopoietic stem cells (CFUs) in the bone marrow. The content of CFUs in the bone marrow and the number of endogenous spleen colonies (ESC) of hemopoietic tissue were higher after irradiation in mice treated with DS than in control groups. The survival rate following a lethal radiation dose was also higher. Injection with HU decreased the number of CFUs in animals injected with DS to the level of controls and suppressed its radioprotective effect.
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
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Vacek
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Bartonícková
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Rotkovská
D000818:Animals; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000528:radiation effects*; D006918:Hydroxyurea / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D015996:Survival Rate
D003911:Dextrans; D011837:Radiation-Protective Agents; D016264:Dextran Sulfate; D006918:Hydroxyurea