Pharmacokinetics and coronary thrombolytic properties of two human tissue-type plasminogen activator variants lacking the finger-like, growth factor-like, and first kringle domains (amino acids 6-173) in a canine model.
The pharmacokinetics and thrombolytic properties of two variants of recombinant human tissue-type plasminogen activator (rt-PA) were studied in dogs with a copper coil induced thrombosis of the left anterior descending coronary artery. The first variant, rt-PA-delta FEK1-Gln184, lacked amino acids 6 to 173 [comprising the fibronectin finger-like (F), the epidermal growth factor-like (E), and the first kringle (K1) domains] and had the glycosylated Asn184 mutagenized to Gln. The second variant, rt-PA-delta FEK1-Gln184, Val277, had in addition Lys277 mutagenized to Val. Injection of 0.25, 0.50, or 1.0 mg/kg of rt-PA in groups of three dogs caused reflow in six of nine dogs, within 18 +/- 15 min (mean +/- SD), but was associated with reocclusion within 2 h in all animals. Injection of 0.125, 0.25, or 0.50 mg/kg of rt-PA-delta FEK1-Gln184 caused reflow in all of nine dogs, within 17 +/- 23 min, with persistent patency in four animals (p = 0.02 vs. rt-PA). Bolus injection of 4:1 mixtures of rt-PA-delta FEK1-Gln184 and rt-PA in total amounts of 0.125, 0.25, or 0.50 mg/kg resulted in reflow in eight of nine dogs within 25 +/- 21 min with persistent patency in seven (p = 0.003 vs. rt-PA, p = 0.25 vs. rt-PA-delta FEK1-Gln184 alone). Injection of 0.25, 0.50, or 1.0 mg/kg of rt-PA-delta FEK1-Gln184, Val277 produced reperfusion in six of nine dogs, within 27 +/- 26 min, with persistent patency in three (p = 0.59 vs. rt-PA and p = 0.23 vs. rt-PA-delta FEK1-Gln184).(ABSTRACT TRUNCATED AT 250 WORDS)
Wu
Z
Z
Laboratory of Experimental Cardiology, K.U. Leuven, Belgium.
Van de Werf
F
F
Stassen
T
T
Mattsson
C
C
Pohl
G
G
Collen
D
D
D000595:Amino Acid Sequence; D000818:Animals; D003328:Coronary Thrombosis / Q000188:drug therapy / Q000503:physiopathology; D004285:Dogs; D005343:Fibrinolytic Agents; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000493:pharmacokinetics / Q000494:pharmacology; D010959:Tissue Plasminogen Activator / Q000235:genetics / Q000493:pharmacokinetics* / Q000494:pharmacology
D005343:Fibrinolytic Agents; D011994:Recombinant Proteins; D010959:Tissue Plasminogen Activator