Molecular cloning, chromosomal assignment, and nucleotide sequence of the feline homeobox HOX3A.
The feline homolog to the mammalian homeobox locus, HOX3A, was isolated by screening a domestic cat genomic library with the murine Hox-3.1 probe. The nucleotide sequence similarity of the feline homeobox was 96% to human HOX3A, 94% to mouse Hox-3.1, and 94% to rat R4. The deduced amino acid sequence (homeodomain) of this feline homeobox was identical to all homeodomains of these cognate genes. Using a panel of feline x rodent somatic cell hybrids, the HOX3A locus was assigned to feline chromosome B4. Human HOX3A and mouse Hox-3.1 have been mapped previously to human chromosome 12 and mouse chromosome 15, respectively, both of which share syntenic homology to feline chromosome B4. These data demonstrate evolutionary conservation of both HOX3A gene sequences and chromosomal location during mammalian evolution.
Laboratory of Viral Carcinogenesis, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201.
R
R
Masuda
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N
Yuhki
S J
SJ
O'Brien
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002415:Cats; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics*; D005801:Genes, Homeobox; D018398:Homeodomain Proteins; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004268:DNA-Binding Proteins; C072785:HOX3A protein, Felis catus; D018398:Homeodomain Proteins; D004247:DNA