Increased intra-articular substance P and prostaglandin E2 following injection of interleukin-1 in rabbits.
Inflammation was induced by intra-articular injection of 100 ng recombinant human interleukin-1 alpha (rhIL-1) into rabbit knees. Substance P (SP) and prostaglandin E2 (PGE2) were measured by radioimmunoassay (RIA) in the joint fluid at 4, 24 and 48 h after rhIL-1 injection. SP was increased by 4 h, further increased at 24 h and remained elevated at 48 h. PGE2 concentration was highest at 4 h and remained elevated at 48 h after rhIL-1 injection. Because of the proinflammatory activities of SP and PGE2, our studies suggest that the elevated SP and PGE2, in the joint may amplify or sustain an initial receptor-mediated inflammatory response to IL-1.
O'Byrne
E M
EM
Research Department, CIBA-GEIGY Corporation, Summit NJ 07901.
Blancuzzi
V J
VJ
Wilson
D E
DE
Wong
M
M
Peppard
J
J
Simke
J P
JP
Jeng
A Y
AY
D000818:Animals; D001168:Arthritis / Q000209:etiology / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism*; D007375:Interleukin-1 / Q000008:administration & dosage / Q000494:pharmacology*; D007596:Joints / Q000378:metabolism; D011817:Rabbits; D013373:Substance P / Q000378:metabolism*; D007507:Therapeutic Irrigation
D007375:Interleukin-1; D013373:Substance P; D015232:Dinoprostone