Neutralization epitopes of human rhinovirus type 2.
Fourteen neutralizing monoclonal antibodies recognizing human rhinovirus (HRV) type 2 have been used to select a total of 51 virus escape mutants. Cross-resistance analysis of the mutants, together with RNA sequencing and identification of amino acid substitutions, have revealed three neutralization sites on the virus surface. Two of these appear to correspond to the NIm-IA and NIm-II sites described for HRV-type 14, although there are also substantial differences. The third site has not been described previously.
Department of Virology, Wellcome Biotechnology Ltd, Beckenham, Kent, U.K.
G
G
Appleyard
S M
SM
Russell
B E
BE
Clarke
S A
SA
Speller
M
M
Trowbridge
J
J
Vadolas
D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D000939:Epitopes; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009500:Neutralization Tests; D012229:Rhinovirus / Q000235:genetics / Q000276:immunology*
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes