Crystallization and preliminary diffraction studies of recombinant human granulocyte-stimulating factor (KW2228).
Human granulocyte colony-stimulating factor (hG-CSF) specifically stimulates proliferation of neutrophils. Two crystal forms of a mutant of hG-CSF expressed in Escherichia coli have been obtained using the hanging drop vapour diffusion method. One form is triclinic, space group P1, with cell dimensions a = 37.3 A, b = 46.4 A, c = 47.7 A, alpha = 105.5 degrees, beta = 98.0 degrees and gamma = 109.4 degrees. The other is monoclinic, space group C2, with cell dimensions a = 82.0 A, b = 49.2 A, c = 49.4 A and beta = 113.9 degrees. Both crystal forms diffract beyond 2.0 A and are suitable for X-ray analysis.
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd., Japan.
Y
Y
Nagahara
N
N
Konishi
Y
Y
Yokoo
N
N
Hirayama
D003115:Colony-Stimulating Factors; D003460:Crystallization; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D011487:Protein Conformation; D011994:Recombinant Proteins; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
D003115:Colony-Stimulating Factors; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor