Aldehyde dehydrogenase induction by glutamate in Escherichia coli. Role of 2-oxoglutarate.
In Escherichia coli, an aldehyde dehydrogenase that catalyzes the oxidation of L-lactaldehyde to L-lactate is induced not only by L-fucose, L-rhamnose or D-arabinose, but also by growth in the presence of glutamate or amino acids yielding glutamate, with the exception of proline. Induction by these amino acids requires glutamate accumulation. 4-Aminobutyric acid also induces this aldehyde dehydrogenase through its transamination to glutamate. Growth on 2-oxoglutarate, the tricarboxylic acid cycle intermediate with which glutamate is in equilibrium, also induces this aldehyde dehydrogenase. Conditions in which the conversion of 2-oxoglutarate into glutamate is highly restricted displayed unchanged rates of induction by 2-oxoglutarate, indicating that glutamate induces the aldehyde dehydrogenase through 2-oxoglutarate formation. Evidence is presented showing that L-fucose- and 2-oxoglutarate-inducing systems share the same regulatory protein. Induction by growth on either of these two compounds is repressed both by glucose and by glycerol. Addition of cAMP to these cultures partially recovers the glucose-repressed aldehyde dehydrogenase activity, while this nucleotide has no effect on the glycerol-mediated repression. These results indicate that ald is under carbon regulation mediated by at least two different mechanisms.
Quintilla
F X
FX
Department of Biochemistry, Faculty of Pharmacy, University of Barcelona, Spain.
Baldoma
L
L
Badia
J
J
Aguilar
J
J
D000444:Aldehyde Dehydrogenase / Q000096:biosynthesis*; D003470:Culture Media; D000242:Cyclic AMP / Q000494:pharmacology; D004790:Enzyme Induction; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D005971:Glutamates / Q000378:metabolism / Q000494:pharmacology*; D018698:Glutamic Acid; D005990:Glycerol / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics
D003470:Culture Media; D005971:Glutamates; D007656:Ketoglutaric Acids; D018698:Glutamic Acid; D000242:Cyclic AMP; D000444:Aldehyde Dehydrogenase; D005990:Glycerol