Stimulation of peripheral blood T cells by an activated T-cell line: a novel human autologous T-T lymphocyte reaction.
T lymphocyte interactions have generally been described between discrete functional subsets. In our investigation of murine T-cell interactions we described a type of T-T interaction termed the 'Syngeneic T-T Lymphocyte Reaction' in which activated T-cell clones stimulated the proliferation of resting T cells mainly through a mechanism involving cell to cell contact. To investigate whether similar reactions occur in the human immune system we used the human autoreactive T-cell line C.1 to stimulate peripheral T cells. Line C.1 cells, which are not transformed and do not secrete IL2, consistently caused proliferation of purified freshly isolated autologous peripheral human T cells as measured by a [3H]-thymidine incorporation assay. The proliferation was seen in both the CD4 and CD8 subsets and could be inhibited with anti-DR and anti-CD2 antibodies. The stimulation is not due to carryover of classical antigen-presenting cells or to the C.1 line cells acting as antigen-presenting cells. We propose that some activated T cells, probably by expression of a surface molecule, can stimulate resting T cells thereby allowing for antigen-non-specific augmentation of the immune response.
Department of Medicine, University of Chicago, Illinois.
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R
Ditzian-Kadanoff
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L
Parks
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B
Evavold
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J
Quintans
T J
TJ
Swartz
D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D018801:CD2 Antigens; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002450:Cell Communication; D002460:Cell Line; D006684:HLA-DR Antigens / Q000502:physiology; D006801:Humans; D008213:Lymphocyte Activation; D011971:Receptors, Immunologic / Q000502:physiology; D016176:T-Lymphocyte Subsets / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology*
D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D015704:CD4 Antigens; D016827:CD8 Antigens; D006684:HLA-DR Antigens; D011971:Receptors, Immunologic