A study of the genomic diversity of Plasmodium falciparum in Senegal. 2. Typing by the use of the polymerase chain reaction.
The genomic polymorphism of Plasmodium falciparum was investigated in a series of samples collected in Senegal during one transmission season. PCR analysis was performed on several genes coding for blood-stage antigens: the gene for the major merozoite surface antigen P190, the gene for the second merozoite surface antigen MSA2 and the gene coding for antigen 96tR/GBP130. In each case, several distinct forms of the genes studied were observed. Both the MAD20 and K1 allelic families of P190 genes were observed. PCR analysis of a single variable region did not differentiate each isolate. However, when the data obtained for several markers are combined, each isolate had a specific genotype. Thus, using PCR to study in parallel several loci is a useful tool to genetically type strains.
Mercereau-Puijalon
O
O
Unité de Parasitologie Expérimentale, Institut Pasteur, Paris, France.
Fandeur
T
T
Bonnefoy
S
S
Jacquemot
C
C
Sarthou
J L
JL
D000483:Alleles; D000818:Animals; D000953:Antigens, Protozoan; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry; D005819:Genetic Markers; D014644:Genetic Variation; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics; D015183:Restriction Mapping; D012675:Senegal
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015342:DNA Probes; D016054:DNA, Protozoan; D005819:Genetic Markers; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium