Phosphorylation of recoverin, the calcium-sensitive activator of photoreceptor guanylyl cyclase.
Recoverin, a new calcium binding protein from bovine rod photoreceptor cells, activates guanylyl cyclase below a free calcium concentration of 200 nM. We show here that recoverin is phosphorylated by an endogenous kinase and Mg-ATP at the same decreased calcium concentration. The calcium-dependent activation of guanylyl cyclase is enhanced in the presence of ATP. We suggest that phosphorylation of recoverin reinforces the stimulation of guanylyl cyclase at decreased calcium concentrations.
Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, Germany.
H G
HG
Lambrecht
K W
KW
Koch
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000378:metabolism* / Q000494:pharmacology; D002417:Cattle; D004789:Enzyme Activation / Q000187:drug effects; D005136:Eye Proteins; D006162:Guanylate Cyclase / Q000378:metabolism*; D051597:Hippocalcin; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D010766:Phosphorylation; D010786:Photoreceptor Cells / Q000201:enzymology*; D011494:Protein Kinases / Q000378:metabolism; D051603:Recoverin; D012374:Rod Cell Outer Segment / Q000737:chemistry
D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D051603:Recoverin; D051597:Hippocalcin; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D006162:Guanylate Cyclase; D002118:Calcium