A rapid and reliable sequential staining method for bovine muscle.
Various different combinations of histochemical stains at differing pH levels on single sections of bovine skeletal muscle were evaluated. The staining sequence that was the most consistent and reliable of those studied included an initial NADH-TR (reduced nicotinamide adenine dinucleotide-tetrazolium reductase) followed by an acid ATPase stain at pH 4.15. This staining combination resulted in easily discernable type I, II A, and II myofibers. Thus, a marked saving of expended time, labor and materials was realized.
Department of Anatomy and Physiology, Kansas State University, Manhattan 66506.
D L
DL
Troyer
R A
RA
Oyster
H W
HW
Leipold
D000818:Animals; D002417:Cattle / Q000033:anatomy & histology*; D005260:Female; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D009132:Muscles / Q000033:anatomy & histology*; D013194:Staining and Labeling