Effect of outer membrane permeability on chemotaxis in Escherichia coli.
The relationship between outer membrane permeability and chemotaxis in Escherichia coli was studied on mutants in the major porin genes ompF and ompC. Both porins allowed passage of amino acids across the outer membrane sufficiently to be sensed by the methyl-accepting chemotaxis proteins, although OmpF was more effective than OmpC. A mutant deleted for both ompF and ompC, AW740, was almost completely nonchemotactic to amino acids in spatial assays. AW740 required greater stimulation with L-aspartate than did the wild type to achieve full methylation of methyl-accepting chemotaxis protein II. Induction of LamB protein allowed taxis to maltose but not to L-aspartate, which indicates that the maltoporin cannot rapidly pass aspartate. Salt taxis was less severely inhibited by the loss of porins than was amino acid taxis, which implies an additional mechanism of outer membrane permeability. These results show that chemotaxis can be used as a sensitive in vivo assay for outer membrane permeability to a range of compounds and imply that E. coli can regulate chemotactic sensitivity by altering the porin composition of the outer membrane.
Ingham
C
C
Department of Biochemistry, University of Wisconsin-Madison 53706.
Buechner
M
M
Adler
J
J
D001224:Aspartic Acid / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002463:Cell Membrane Permeability; D002633:Chemotaxis; D002712:Chlorides / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D015636:Magnesium Chloride / Q000494:pharmacology; D008320:Maltose / Q000494:pharmacology; D009154:Mutation; D010957:Plasmids; D018272:Porins; D012694:Serine / Q000494:pharmacology
D001425:Bacterial Outer Membrane Proteins; D002712:Chlorides; D007473:Ion Channels; D018272:Porins; D015636:Magnesium Chloride; D001224:Aspartic Acid; D012694:Serine; D008320:Maltose; D008094:Lithium; D018021:Lithium Chloride