Detection of human papillomavirus DNA in gynaecological swabs by filter in situ hybridization.
Gynaecological smears from the endo- and ectocervix of women with and without cytological and colposcopic abnormalities of the epithelium were investigated for human papillomavirus (HPV) types 6, 11, 16, and 18 by filter in situ hybridization (FISH). The data were compared with cytological, colposcopic, and histological findings. Of the 266 gynaecological smears, HPV DNA was detected in 84 (32%); of 101 cytologically and colposcopically HPV negative cases, HPV DNA was found in 10%. Of 56 women, cytologically and colposcopically positive for HPV infection, HPV DNA was detected in 68%. The sensitivity of the method was controlled by comparing the results of FISH with those of Southern-blot analysis of five cervical tumour biopsies. The data presented demonstrate the necessity of FISH for identification of the HPV type that might be of prognostic value in cervical pathology. Cytological and colposcopic positivity is a reliable sign in about 70% of the cases where HPV infection was proved by FISH.
Mincheva
A
A
Institute of Infectious and Parasitic Diseases, Medical Academy, Sofia, Bulgaria.
Shindarov
L
L
Karageosov
I
I
Slavchev
B
B
Bachvarov
D
D
Vasilev
N
N
Makaveeva
V
V
D000293:Adolescent; D000328:Adult; D002584:Cervix Uteri / Q000382:microbiology*; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000382:microbiology; D014626:Vaginal Smears
D004279:DNA, Viral