Acute phase protein stimulation by alpha 1-antichymotrypsin-cathepsin G complexes. Evidence for the involvement of interleukin-6.
Incubation of alpha 1-antichymotrypsin-cathepsin G complexes with human lung fibroblasts caused a nearly 5-fold increase in synthesis of the cytokine interleukin-6. In turn, the fibroblast-conditioned medium induced significant synthesis of the acute phase proteins haptoglobin, fibrinogen, and alpha 1-antichymotrypsin in human Hep G2 cells, whereas a mixture of interleukin-1 and conditioned medium was considerably less stimulatory. These data indicate that proteinase-proteinase inhibitor complexes formed between plasma serpins and their target enzymes could play major roles in signaling for acute phase protein synthesis in response to injury.
Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.
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Kurdowska
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Travis
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D056649:Cathepsin G; D002403:Cathepsins / Q000494:pharmacology*; D002460:Cell Line; D005340:Fibrinogen / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D006242:Haptoglobins / Q000096:biosynthesis; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000096:biosynthesis*; D007700:Kinetics; D011994:Recombinant Proteins / Q000494:pharmacology; D012697:Serine Endopeptidases; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D000514:alpha 1-Antichymotrypsin / Q000494:pharmacology*
D000209:Acute-Phase Proteins; D006242:Haptoglobins; D007375:Interleukin-1; D015850:Interleukin-6; D011994:Recombinant Proteins; D000514:alpha 1-Antichymotrypsin; D005340:Fibrinogen; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D013755:Tetradecanoylphorbol Acetate