Oral transmission of Jamestown Canyon virus by Aedes provocans mosquitoes from northeastern New York.
Aedes provocans were allowed to feed on a bloodmeal containing 5.6 log10 TCID50/ml of Jamestown Canyon (JC) virus. After 14 days of incubation at 21 degrees C and 80% RH, 100% (36/36) were midgut infected, 50% (18/36) developed disseminated infections and 50% (9/18) of the latter specimens transmitted virus to capillary tubes. When mosquitoes were intrathoracically inoculated with 6.1 log10 TCID50/ml of JC virus, 100% (40/40) became disseminated infected and 95% (38/40) transmitted virus after 12 days of incubation. A midgut escape barrier was recognized as the major barrier to JC virus transmission in orally infected Ae. provocans.
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
R D
RD
Boromisa
M A
MA
Grayson
D000818:Animals; D009033:Culicidae / Q000382:microbiology*; D002141:Encephalitis Virus, California / Q000302:isolation & purification; D004670:Encephalitis, California / Q000635:transmission*; D005260:Female; D007303:Insect Vectors; D009518:New York; D012463:Saliva / Q000382:microbiology