Integrated dystrophin analysis using immunocytochemical, biochemical and genetic techniques.
Immunocytochemistry is a powerful tool for the elucidation of clinical problems, particularly when used in conjunction with complementary techniques such as immunoblotting and DNA analysis. We would like to present an experimental example which illustrates the way these three different disciplines were brought together to investigate the defective gene product in X-linked muscular dystrophy.
Muscular Dystrophy Group Research Laboratories, Newcastle General Hospital, Newcastle upon Tyne, U.K.
L V
LV
Nicholson
M A
MA
Johnson
K E
KE
Davies
D002872:Chromosome Deletion; D016189:Dystrophin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000939:Epitopes; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009132:Muscles / Q000378:metabolism; D009136:Muscular Dystrophies / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics
D016189:Dystrophin; D000939:Epitopes