[A simple method for the comparative evaluation of the rate of reparative synthesis in cultured mammalian cells].
A sensitive method for estimating the rate of repair synthesis is proposed. The method is based on the capacity of long-wave UV light to inactivate cells having minor amounts of bromodeoxyuridine (BUdR) incorporated. This treatment is absolutely nontoxic for cells lacking BUdR. The efficacy of the treatment is achieved by sensitizing cells with a fluorescent dye Hoechst 33258. The proposed method has proved to be effective in studying the repair synthesis in HeLa cells after exposure to short-wave UV light and gamma rays.
P G
PG
Strel'tsov
M V
MV
Filatov
D000818:Animals; D006690:Bisbenzimidazole / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003584:Cytological Techniques; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects*; D005720:Gamma Rays; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D014466:Ultraviolet Rays
D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole
Prostoĭ metod sravnitel'noĭ otsenki skorosti reparativnogo sinteza v kul'tiviruemykh kletkakh mlekopitaiushchikh.