Ethanol enhancement of GABA-activated chloride current in rat dorsal root ganglion neurons.
The acute effects of ethanol on the gamma-aminobutyric acid (GABA)-activated current were studied with the rat dorsal root ganglion neurons in primary culture using the whole-cell patch-clamp technique. GABA produced an inward chloride current, which was composed of an initial transient and a subsequent sustained phase. Ethanol at concentrations ranging from 30 to 300 mM enhanced the transient current in a concentration-dependent manner without affecting the sustained current.
Department of Pharmacology, Northwestern University Medical School, Chicago, IL 60611.
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Nishio
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Narahashi
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D018118:Chloride Channels; D004553:Electric Conductivity; D000431:Ethanol / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000502:physiology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D018118:Chloride Channels; D007473:Ion Channels; D008565:Membrane Proteins; D000431:Ethanol; D005680:gamma-Aminobutyric Acid