Isolation and characterization of DNA from archaeological bone.
DNA was extracted from human and animal bones recovered from archaeological sites and mitochondrial DNA sequences were amplified from the extracts using the polymerase chain reaction. Evidence is presented that the amplified sequences are authentic and do not represent contamination by extraneous DNA. The results show that significant amounts of genetic information can survive for long periods in bone, and have important implications for evolutionary genetics, anthropology and forensic science.
MRC Molecular Haematology Unit, University of Oxford, John Radcliffe Hospital, U.K.
E
E
Hagelberg
J B
JB
Clegg
D000818:Animals; D001483:Base Sequence; D001842:Bone and Bones / Q000737:chemistry*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification; D005580:Fossils; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004272:DNA, Mitochondrial; D004247:DNA