Normal human chromosome 1 carries suppressor activity for various phenotypes of a Kirsten murine sarcoma virus-transformed NIH/3T3 cell line.
In order to identify chromosomes that carry putative tumor-suppressor genes for the various phenotypes of Kirsten sarcoma virus-transformed NIH/3T3 (DT) cells, we performed microcell-mediated chromosome transfer into DT cells. We first isolated mouse A9 clones, containing a single human chromosome 1, 11 or 12 tagged with pSV2-neo plasmid DNA. Then, chromosome 1, 11 or 12 was transferred from the A9 clones into DT cells by microcell fusion. The growth rate, colony-forming ability in soft agar and tumorigenicity of the DT cells were controlled by chromosome 1, but not by chromosome 11 or 12, indicating that normal human chromosome 1 carries a putative tumor-suppressor gene(s) that affects various transformed phenotypes of DT cells.
Department of Molecular and Cell Genetics, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago.
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Yamada
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Horikawa
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Hashiba
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Oshimura
D000818:Animals; D015152:Blotting, Northern; D002472:Cell Transformation, Viral / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1; D002880:Chromosomes, Human, Pair 11; D002881:Chromosomes, Human, Pair 12; D003114:Colony-Forming Units Assay; D015972:Gene Expression Regulation, Neoplastic; D016153:Genes, Suppressor; D006801:Humans; D006822:Hybrid Cells; D066298:In Vitro Techniques; D007708:Kirsten murine sarcoma virus / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D008858:Microscopy, Phase-Contrast; D012313:RNA / Q000032:analysis; D012513:Sarcoma, Experimental / Q000235:genetics*; D014170:Transformation, Genetic
D012313:RNA