Quantification of glutamate, glutamine, and other metabolites in in vivo proton NMR spectroscopy.
A reliable quantification of in vivo 1H MRS spectra is hampered by extensive line overlap, by intensity distortions due to the water suppressing pulse sequence and complex modulation patterns of J-coupled spins, and by deviations between the experimental and model lineshapes in computer fitting programs. By correcting the experimental lineshape, using a suitable pulse sequence, and incorporating prior knowledge about the spin systems and the intensity distortions, a sufficient quantification is possible. If reliable T2 values are available absolute concentrations can be determined.
Delft University of Technology, Department of Applied physics, The Netherlands.
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D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000032:analysis; D002540:Cerebral Cortex / Q000737:chemistry*; D005971:Glutamates / Q000032:analysis*; D018698:Glutamic Acid; D005973:Glutamine / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011522:Protons; D051381:Rats
D005971:Glutamates; D011522:Protons; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; C000179:N-acetylaspartate