Expression of a sperm protein gene during spermatogenesis in mammalian testis: an in situ hybridization study.
In previous work a specific membrane protein with an estimated Mr of 20.1 kDa was purified from rabbit sperm tails and designated as rSMP-B protein. Antibodies were raised against rSMP-B protein and used to isolate and identify the cDNA coding the rSMP-B protein from a rat testis lambda gt11 expression library. The nucleotide sequence of the cDNA was determined in a previous study. Single-stranded 35S-labeled RNA probes were prepared. With the techniques of in situ hybridization, rSMP-B mRNA was detected in spermatids of rat and rabbit testis. The present results support our previous observation that immunization of male rabbits with the rSMP-B protein results in the arrest of spermatogenesis at the spermatid stage. Overall, rSMP-B protein appears to be involved in spermiogenesis, and the synthesis of the mRNA encoding the protein occurs in germ cells during the postmeiotic haploid phase of spermatogenesis.
Wang
L F
LF
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
Miao
S Y
SY
Yan
Y C
YC
Li
Y H
YH
Zong
C
C
Koide
S S
SS
D000818:Animals; D000954:Antigens, Surface; D015870:Gene Expression; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012313:RNA; D018075:RNA, Complementary; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011817:Rabbits; D051381:Rats; D013082:Sperm Tail / Q000378:metabolism*; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
D000954:Antigens, Surface; D008565:Membrane Proteins; D018075:RNA, Complementary; D012333:RNA, Messenger; C049242:rSMP-B protein, human; D012313:RNA