Protein metabolism after injury with turpentine: a rat model for clinical trauma.
Subcutaneous injections of turpentine induced discrete aseptic abscesses in rats without detectable injury to other tissues. Hypoalbuminemia and high circulating concentrations of alpha 2-macroglobulin were present after 48 h. Liver size, protein content, and protein fractional synthetic rate (FSR) were all increased, whereas loss of muscle protein occurred together with similar (45-50%) reductions in both intramuscular glutamine concentrations ([Gln]i, mmol/l intracellular water) and FSR. The rats became anorexic, but pair feeding (50% ad libitum for 48 h) produced a 22% increase in muscle [Gln]i and only an 18% reduction in muscle FSR. Dietary restriction therefore did not demonstrate the positive relationship between muscle [Gln]i and FSR, which has been observed in animal trauma studies. It is concluded that subcutaneous injections of turpentine produce many of the features of the acute-phase response to injury, and therefore this technique is recommended as a convenient laboratory model for studying various aspects of the protein metabolic response to injury.
Medical Research Council Dunn Nutrition Unit, Cambridge, United Kingdom.
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Wusteman
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Wight
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Elia
D000210:Acute-Phase Reaction / Q000378:metabolism*; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D004195:Disease Models, Animal; D007249:Inflammation; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D009336:Necrosis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014425:Turpentine / Q000633:toxicity*
D011506:Proteins; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase; D000469:Alkaline Phosphatase; D001663:Bilirubin; D014425:Turpentine