Measurement of the 'fast' or complexed form of alpha 2 macroglobulin in biological fluids using a sandwich enzyme immunoassay.
A sandwich enzyme immunoassay has been developed for measuring the 'fast' or complexed form of alpha 2 macroglobulin using a complex-specific monoclonal antibody. The working range of this assay is 1.5-15 micrograms/l and is suitable for use with various biological fluids. Using this assay the normal plasma levels were found to range from 4.2 mg/l to 14.4 mg/l (0.17%-0.70% of total alpha 2 macroglobulin) with a mean value of 7.6 mg/l +/- 2.6 (0.37% +/- 0.12% of total alpha 2 macroglobulin). Elevated levels were seen in plasma samples taken on the day of admission from patients with acute pancreatitis and in some synovial fluid samples from patients with various arthritides.
Department of Chemical Pathology, University of Leeds, U.K.
R E
RE
Banks
S W
SW
Evans
F
F
Van Leuven
D
D
Alexander
M J
MJ
McMahon
J T
JT
Whicher
D000911:Antibodies, Monoclonal; D000925:Anticoagulants / Q000494:pharmacology; D001168:Arthritis / Q000378:metabolism; D010450:Endopeptidases / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010195:Pancreatitis / Q000097:blood; D013582:Synovial Fluid / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000925:Anticoagulants; D000511:alpha-Macroglobulins; D010450:Endopeptidases