Splenic noradrenergic and adrenocortical responses during the preclinical and clinical stages of adoptively transferred experimental autoimmune encephalomyelitis (EAE).
When experimental autoimmune encephalomyelitis (EAE) is induced by adoptive transfer of myelin basic protein (MBP)-specific lymphocytes the splenic noradrenergic and adrenocortical responses mirror in most respects those that occur following sensitization with spinal cord and Freund's adjuvant (CFA), despite the absence of the primary immune challenge. An early drop in splenic noradrenaline (NA), observed only when purified protein derivative-primed cells are transferred may reflect a vigorous proliferative response in vitro, not observed with MBP-specific cells. However, serum corticosterone (CS) levels and the density of splenocyte beta-adrenergic receptors were increased in both experimental groups within 3 days of cell inoculation. The stress of clinical signs of EAE resulted in highly significant increases in both splenic NA and plasma CS. Thus adoptively transferred EAE provides a well-delineated model of autoimmune disease for investigating the immunomodulatory role of the neural and endocrine systems.
Department of Neurochemistry, Institute of Neurology, London, U.K.
J P
JP
Leonard
F J
FJ
MacKenzie
H A
HA
Patel
M L
ML
Cuzner
D000302:Adrenal Cortex / Q000378:metabolism*; D000818:Animals; D003345:Corticosterone / Q000097:blood / Q000494:pharmacology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D005260:Female; D007114:Immunization; D007116:Immunization, Passive; D008214:Lymphocytes / Q000378:metabolism; D004676:Myelin Basic Protein / Q000276:immunology; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D013997:Time Factors
D004676:Myelin Basic Protein; D011943:Receptors, Adrenergic, beta; D003345:Corticosterone; D009638:Norepinephrine