Specific interaction of CD4-bearing liposomes with HIV-infected cells.
The CD4 molecule was reconstituted into the bilayers of large liposomes. Fluorescence microscopy and electron microscopy showed that these liposomes interact with HIV-infected H9-HT cells, delivering their contents to the cell interior. Liposomes bearing CD4 did not interact in this way with noninfected H9-HT cells nor did liposomes without CD4 interact significantly with HIV-infected cells. From electron micrographs, it appeared that HIV binds to liposomes bearing CD4; no attachment of virions to liposomes without CD4 was observed.
Biophor Corporation, College Station, Texas.
A
A
Cudd
C A
CA
Noonan
P F
PF
Tosi
J L
JL
Melnick
C
C
Nicolau
D015704:CD4 Antigens / Q000276:immunology*; D002460:Cell Line; D000939:Epitopes / Q000276:immunology; D005614:Freeze Fracturing; D006678:HIV / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008081:Liposomes / Q000276:immunology / Q000378:metabolism*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D014771:Virion / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure
D015704:CD4 Antigens; D000939:Epitopes; D008081:Liposomes