Identification of a rat liver dipeptidyl aminopeptidase IV with a liver plasma membrane glycoprotein (gp110). A study using dipeptidyl aminopeptidase IV-deficient rats.
A rat liver plasma membrane glycoprotein, gp110, was compared with dipeptidyl aminopeptidase IV (DAP IV) by using Wistar rats (DAP IV-positive rats) and Fischer 344 rats (DAP IV-negative rats). Fischer rats also lacked gp110 and gp110 of Wistar rats had DAP IV activity. Furthermore, we showed that the C-terminal sequence of gp110 was Ser-Leu-Arg, which was the same as the C-terminal amino acid sequence deduced from the nucleotide sequence of the cDNA of DAP IV. According to these results, we concluded that gp110 was identical with DAP IV.
Department of Clinical Biochemistry, Hokkaido Institute of Pharmaceutical Sciences, Japan.
S
S
Iwaki-Egawa
Y
Y
Watanabe
Y
Y
Fujimoto
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000172:deficiency; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000737:chemistry* / Q000172:deficiency; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D016415:Sequence Alignment
D008562:Membrane Glycoproteins; C057630:canaliculus domain-specific glycoprotein gp110; D000626:Aminopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4