Detection of viruses using hybridization techniques.
Nucleic acid hybridization provides a direct means of detection of viruses, their genomes or the transcripts found in infected cells. A brief introduction to the biochemistry involved is given in this article. Examples of the methods available are illustrated by the detection of hepatitis B viruses both in serum samples and at the site of replication, the liver.
Academic Department of Medicine, Royal Free Hospital School of Medicine, London, UK.
T J
TJ
Harrison
D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis; D006509:Hepatitis B / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D016036:Seroepidemiologic Studies
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens