On the use of T7 RNA polymerase transcripts for physical investigation.
A few years ago we made some observations which raised questions about the accuracy with which T7 RNA polymerase transcribes templates in vitro, and the suitability of its in vitro products for biophysical study (1). The experiments described below demonstrate that there is no reason for concern; the products of T7 RNA polymerase transcription in vitro are as suitable for biophysical characterization as RNAs synthesized in vivo. It is likely that aggregation involving the transcribed portions of the T7 RNA polymerase promoter caused our initial observations.
Szewczak
A A
AA
Department of Chemistry, Yale University, New Haven, CT 06511.
White
S A
SA
Gewirth
D T
DT
Moore
P B
PB
D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D013604:T-Phages / Q000201:enzymology* / Q000235:genetics; D013698:Templates, Genetic; D014158:Transcription, Genetic
D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases