cDNA cloning of a homeobox-containing gene expressed in avian myeloblastic virus-transformed chicken monoblastic leukaemia cells.
By combining the polymerase chain reaction and differential library screening, a cDNA for an mRNA expressed in chicken avian myeloblastosis virus (AMV)-transformed monoblasts was isolated. This mRNA is not expressed in erythroblast or T-lymphoblast cell lines. Induced differentiation of the cells of the AMV-transformed BM2 line was associated with reduced levels of this transcript. The predicted protein product of Chox M was a homeodomain factor similar to murine Hox-4.3.
Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
M R
MR
Crompton
A D
AD
MacGregor
G H
GH
Goodwin
D000595:Amino Acid Sequence; D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000235:genetics*; D001483:Base Sequence; D002472:Cell Transformation, Viral / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005801:Genes, Homeobox / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D004247:DNA