Cellular localization of an embryonic interferon, ovine trophoblastin and its mRNA in sheep embryos during early pregnancy.
The ovine embryo produces an interferon named ovine Trophoblastin (oTP) which is involved in the maternal recognition of pregnancy and ensures the maintenance of progesterone secretion by the corpus luteum. We have used indirect immunohistofluorescence and in situ hybridization on histological sections to investigate the fate of this protein and its mRNA in ovine embryos from days 3 to 25 of pregnancy. The level of expression was measured by image analysis of the autoradiographs after in situ hybridization. Both techniques clearly demonstrated that oTP and its mRNA were specifically localized in the extra-embryonic trophoblast. Neither the embryonic cells, nor the yolk sac or the amniotic tissues produced the protein or its mRNA. The protein could be detected by d 11 of pregnancy in the elongated blastocyst. Maximum of expression is observed at d 14 and the level decreased by d 16 of pregnancy. The arrest of expression occurred in the regions of trophoblast which have established cellular contacts with the uterine epithelium during the implantation process.
Unité Endocrinologie de l'Embryon, Station de Physiologie animale, Villejuif, France.
M
M
Guillomot
C
C
Michel
P
P
Gaye
N
N
Charlier
J
J
Trojan
J
J
Martal
D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D007370:Interferon Type I; D007372:Interferons / Q000235:genetics / Q000378:metabolism*; D008186:Luteolytic Agents / Q000037:antagonists & inhibitors*; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism*; D011270:Pregnancy, Animal / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012756:Sheep; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism
D007370:Interferon Type I; D008186:Luteolytic Agents; D011257:Pregnancy Proteins; D012333:RNA, Messenger; C020947:interferon tau; D007372:Interferons