Identification of the region of T cell receptor beta chain that interacts with the self-superantigen MIs-1a.
Superantigen-MHC complexes are known to stimulate T cells primarily via the V beta element of the T cell receptor. In this paper we identify a number of amino acid residues that define the region of a particular V beta element interacting with one of the self-superantigens, MIs-1a. These residues are predicted to lie on a beta-pleated sheet of the T cell receptor, away from the complementarity determining regions of the receptor, which are thought to interact with complexes of conventional peptide antigens and MHC. In support of this prediction, mutations affecting MIs-1a activity have no effect on the response to conventional antigen and MHC.
Howard Hughes Medical Institute, Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
A M
AM
Pullen
T
T
Wade
P
P
Marrack
J W
JW
Kappler
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D014644:Genetic Variation; D046911:Macromolecular Substances; D008285:Major Histocompatibility Complex; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D016747:Minor Lymphocyte Stimulatory Antigens; D008958:Models, Molecular; D009154:Mutation; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D015183:Restriction Mapping; D014162:Transfection
D000954:Antigens, Surface; D000939:Epitopes; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D016747:Minor Lymphocyte Stimulatory Antigens; D011948:Receptors, Antigen, T-Cell