Binding of CD 35, CD 21 and CD 11B monoclonal antibodies and EAC complexes to lymphocytes. A comparative histochemical study.
Using cryostat sections of human tonsils after various pretreatments, the adherence of sheep erythrocyte--antisheep erythrocyte (EA) complexes coated with either fresh mouse complement (EACm) or fresh human complement (EACh) has been compared with the binding of monoclonal antibodies (MAbs) against C3b (CD 35), C3d (CD 21) and iC3b (CD 11b) receptors (clusters). After periodic acid oxidation the binding of MAbs to CD 35, CD 21 was well preserved, whereas EACm and EACh complex adherence and CD 11b binding were abolished. These findings seem to prove that the binding sites for EACm and EACh complexes, as well as those for CD 35, CD 21 clusters (antigenic structure of the C3b and C3d receptors) are different.
Department of Pathology, University Medical School of Pécs, Hungary.
F
F
Baranyay
T
T
Magyarlaki
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology; D001665:Binding Sites; D018845:CD11 Antigens; D003165:Complement System Proteins / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D014066:Palatine Tonsil / Q000276:immunology*; D010504:Periodic Acid / Q000494:pharmacology; D011951:Receptors, Complement / Q000276:immunology*; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D018845:CD11 Antigens; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d; D010504:Periodic Acid; D003165:Complement System Proteins