Linkage analysis of the nuclear homologues of mitochondrial plasmid-like DNAs in rice.
A genetical study on the nucleotide sequences of the nuclear DNAs which share homology with rice mitochondrial plasmid-like DNAs, B1, B2, B3 and B4 was carried out. Restriction fragments of the nuclear DNAs hybridized with these plasmid-like DNAs showed polymorphisms in their length between Indica and Japonica rice cultivars. The hybridized signals found specifically in Indica or Japonica cultivars segregated in the F2 population derived from a cross between these two subspecies. The observed ratio of the nuclear homologues in the F2 population demonstrated that they were transmitted according to the Mendelian inheritance. The co-segregation of homologues was examined and the linkage was detected between the B1-nuclear homologue of Japonica and the B4-nuclear homologue of Indica, and also between the nuclear homologues of B2 and B3 of Indica. The linkage between the B1-nuclear homologue of Japonica and the B4-nuclear homologue of Indica was conserved in the different rice cultivars.
Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo, Japan.
A
A
Kanazawa
W
W
Sakamoto
N
N
Kishimoto
M
M
Yano
N
N
Tsutsumi
A
A
Saito
S
S
Tano
D015139:Blotting, Southern; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004270:DNA, Circular / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D008040:Genetic Linkage; D012275:Oryza / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid
D004270:DNA, Circular; D004272:DNA, Mitochondrial