Restriction fragment length polymorphism analysis of endogenous avian leukosis viral loci: determination of frequencies in commercial broiler lines.
Four component lines of a commercial broiler stock were analyzed by restriction fragment length polymorphism (RFLP) analysis, for the presence of endogenous avian leukosis virus loci. The resulting RFLP patterns of endogenous virus (ev) loci for the four strains were further characterized in terms of number of loci per animal and frequency. Loci were also analyzed within limits for major structural alterations and deletions to discern whether certain loci were similar to previously identified loci in White Leghorn layer birds. The ev RFLP patterns for these broiler lines were found to be highly complex and contained many loci unreported in White Leghorn layer birds.
Boulliou
A
A
Laboratoire de Génétique Moléculaire, URA 256 CNRS, Université de Rennes, France.
Le Pennec
J P
JP
Hubert
G
G
Donal
R
R
Smiley
M
M
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D015139:Blotting, Southern; D002645:Chickens / Q000235:genetics / Q000382:microbiology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D005787:Gene Frequency; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D015342:DNA Probes; D004279:DNA, Viral