Limited N-terminal sequence analysis.
Current methods in protein purification permit sequence analysis of picomole quantities of proteins and peptides. The key to obtaining sequence is to choose as the last step in the purification protocol the simplest (usually the fastest) method for isolating the protein or peptide.
Matsudaira
P
P
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007202:Indicators and Reagents; D008722:Methods; D010446:Peptide Fragments / Q000302:isolation & purification; D010447:Peptide Hydrolases; D010455:Peptides / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D013194:Staining and Labeling
D007202:Indicators and Reagents; D010446:Peptide Fragments; D010455:Peptides; D011506:Proteins; D010447:Peptide Hydrolases