A growth arrest-specific (gas) gene codes for a membrane protein.
A set of growth arrest-specific (gas) genes whose expression is negatively regulated by serum has recently been identified. We report on the detailed analysis of one of these genes (gas3). The kinetics of regulation by the presence and absence of serum were investigated, and it was found that this gene is regulated at the post-transcriptional level. The encoded protein deduced from the nucleotide sequence showed some similarity to a mitochondrial oxyreductase, and in vitro translation established that the protein product is a transmembrane glycoprotein.
International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
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G
Manfioletti
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ME
Ruaro
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Del Sal
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L
Philipson
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Schneider
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003470:Culture Media; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004261:DNA Replication; D015723:Gene Library; D005796:Genes; D007700:Kinetics; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone); D014176:Protein Biosynthesis; D011808:Quinone Reductases / Q000235:genetics; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D003470:Culture Media; D046911:Macromolecular Substances; D008565:Membrane Proteins; D012333:RNA, Messenger; D012313:RNA; D004247:DNA; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases