[Immunochemical and biological properties of synthetic peptide fragments from the 124-144 sequence of human leukocyte interferon alpha2].
Three peptides corresponding to the sequences 124-144, 124-138, 129-144 of the human leukocyte interferon alpha 2 (IFN-alpha 2) were synthesized. The synthesis was performed by DCC-HOBT coupling of protected peptide segments in solution. The segments were obtained by the active ester coupling methodology using base-labile 2-[4-(phenylazobenzyl)sulfonyl]ethyl (Pse) group as carboxyterminal protection. After complete deprotection with 1 M methanesulphonic acid in trifluoroacetic acid--thioanisol--m-cresol mixture the peptides were purified by reversed-phase chromatography. The studies of interaction of the peptides with rabbit antiserum against IFN-alpha 2 revealed at least one minor antigenic determinant within the 124-144 region of IFN-alpha 2 amino acid sequence. Rabbit antisera developed against peptides 124-138 and 129-144 showed ability of binding recombinant IFN-alpha 2 and neutralizing its antiviral activity. Free peptides or their conjugates with bovine serum albumine did not display antiviral activity, neither could they inhibit the activity of IFN-alpha 2.
A F
AF
Sheval'e
V V
VV
Samukov
V I
VI
Ofitserov
V V
VV
Kalashnikov
G A
GA
Mizenko
A A
AA
Kolokol'tsov
D000595:Amino Acid Sequence; D000998:Antiviral Agents; D001667:Binding, Competitive; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D007120:Immunochemistry; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D011994:Recombinant Proteins
D000998:Antiviral Agents; D000939:Epitopes; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D010446:Peptide Fragments; D011994:Recombinant Proteins
Immunokhimicheskie i biologicheskie svoĭstva sinteticheskikh peptidnykh fragmentov posledovatel'nosti 124-144 leĭkotsitarnogo interferona cheloveka alpha2.