Nutritional and endocrinological manipulation of lean deposition in forage-fed steers.
The effect of supplementing grass silage with fishmeal on growth, muscle composition and the rate of muscle protein synthesis was investigated in young Friesian steers with and without oestradiol implants. The effect of the beta-adrenergic agonist cimaterol was simultaneously investigated in animals fed on silage alone. Treatments lasted for 9 or 10 weeks. Fishmeal supplementation significantly increased animal growth rates (P less than 0.001) and the weights of three dissected muscles (P less than 0.001) compared with the silage-fed controls. These effects were further enhanced in animals also implanted with oestradiol. Muscle weights expressed as a proportion of body-weight were increased by fishmeal, suggesting that protein deposition had been enhanced. No further increase in the proportional muscle weights was obtained with oestradiol. Muscle dry matter content tended to be increased in both implanted and non-implanted animals receiving fishmeal compared with controls, but the proportions of protein, fat and ash were relatively constant. The intramuscular lipid composition was slightly altered by fishmeal. Muscle protein fractional synthetic rates (FSR), measured by continuous infusion of [3H]tyrosine, were increased by fishmeal in all three muscles of both implanted and non-implanted animals. There were no differences, however, due to oestradiol, over non-implanted fishmeal animals. This suggests that oestradiol may increase muscle accretion by reducing protein degradation rate. Cimaterol significantly increased longissimus dorsi (P less than 0.05) and vastus lateralis (P less than 0.01) muscle weights but had no effect on semitendinosus muscle weight or live-weight gain. The proportion of protein was increased (P less than 0.001) and the fat content reduced (P less than 0.05) in all three muscles but intramuscular lipid composition was not markedly affected. Whilst methylhistidine: creatinine excretion was reduced by cimaterol, FSR were increased in the l. dorsi and v. lateralis muscles suggesting beta-agonists have effects on both protein synthesis and protein degradation.
Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics.
J M
JM
Dawson
P J
PJ
Buttery
M J
MJ
Lammiman
J B
JB
Soar
C P
CP
Essex
M
M
Gill
D E
DE
Beever
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002417:Cattle / Q000254:growth & development* / Q000378:metabolism; D004044:Dietary Proteins / Q000008:administration & dosage*; D004958:Estradiol / Q000008:administration & dosage*; D004983:Ethanolamines / Q000494:pharmacology; D005396:Fish Products; D050356:Lipid Metabolism; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis; D009132:Muscles / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D012820:Silage
D000318:Adrenergic beta-Agonists; D004044:Dietary Proteins; D004983:Ethanolamines; D009124:Muscle Proteins; D004958:Estradiol; C047411:cimaterol