[The ts phenotype of reisolates from children inoculated with live cold-adapted influenza vaccine type A].
Using mutants of fowl plague virus (FRV) which have single temperature-sensitive (ts) mutations in some genes, an analysis was carried out on reisolates from children of 3-6 years, vaccinated with a monovaccine from recombinant strains of influenza type A virus. The recombinants were obtained by crossing of current epidemic strains of subtypes A (HINI) and a (H3N2) with the cold-adapted (XA) ts-donor of attenuation A/Leningrad/134/47/57 (H2N2) from which they, as a rule, inherited 5 ts-mutations in genes 1 (PB2), 2 (PB1), 5 (NP), 7 (M), and 8 (NS). All the reisolates were shown to retain the ts-phenotype. However, in the recombination test some reisolates (most frequently those isolated at late periods of vaccination infection) no ts-mutations could be found in 1-3 genes coding for proteins of the polymerase complex, less frequently for NP and NS proteins but not for M proteins.
Medvedeva
T E
TE
Romanova
Iu R
IuR
Gushchina
M I
MI
Rudenko
L G
LG
Gendon
Iu Z
IuZ
Aleksandrova
G I
GI
Klimov
A I
AI
D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003080:Cold Temperature; D000939:Epitopes / Q000032:analysis / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic / Q000235:genetics; D012692:Serial Passage; D013696:Temperature; D013997:Time Factors
D000956:Antigens, Viral; D000939:Epitopes; D007252:Influenza Vaccines
ts-fenotip reizoliatov ot deteĭ, privitykh zhivoĭ kholodoadaptirovannoĭ grippoznoĭ vaktsinoĭ tipa A.