Characterization of an antigen defined by monoclonal antibody KMO1.
We have isolated an antigen defined by the monoclonal antibody KMO1 from tissue culture supernatant as a glycoprotein and from cancer cells as a glycolipid. The antigenic determinant was a carbohydrate. Antibody KMO1 inhibited the binding activity of 19-9 to CA19-9, but 19-9 antibody did not inhibited the binding activity of KMO1 to KMO1 antigen. The glycolipid antigen of KMO1 was a monosialoganglioside and was separated into 3 components on thin layer chromatography. The 19-9 antibody reacted with one of the components. These results suggest that the KMO1 antigen is similar to but not identical to CA19-9.
Kano
Y
Y
Research Division, Green Cross Corporation, Hirakata, Japan.
Taniguchi
T
T
Uemura
Y
Y
Yokoyama
K
K
Uesaka
K
K
Yamamoto
M
M
Ohyanagi
H
H
Saitoh
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000097:blood / Q000302:isolation & purification*; D014408:Biomarkers, Tumor / Q000276:immunology / Q000302:isolation & purification*; D002241:Carbohydrates / Q000276:immunology; D002846:Chromatography, Affinity; D000939:Epitopes / Q000276:immunology; D005732:Gangliosides / Q000276:immunology / Q000302:isolation & purification; D006017:Glycolipids / Q000276:immunology / Q000302:isolation & purification; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002241:Carbohydrates; D000939:Epitopes; D005732:Gangliosides; D006017:Glycolipids; D006023:Glycoproteins