Characterization of liver cytokeratin as a major target antigen of anti-SLA antibodies.
Anti-SLA antibodies characterize a newly defined subgroup of patients with autoimmune chronic active hepatitis. The aim of the present study was the immunochemical characterization of the target antigen(s) of anti-SLA antibodies. Anti-SLA-positive sera were found to contain high titres of anti-cytokeratin antibodies. In immunoblotting analyses with 100,000 x g supernatants of human liver homogenates (S-100) these sera recognized various proteins with a molecular mass of 40-60 kDa. These proteins were also recognized by monoclonal anti-cytokeratin antibodies. Two-dimensional co-electrophoresis and immunoblotting analysis of S-100 and liver cytokeratins showed that anti-SLA antibodies were primarily directed against cytokeratins, particularly against liver cytokeratin types 8 and 18. This was supported by affinity chromatography, immunofluorescence and absorption methods using anti-SLA sera.
Wächter
B
B
I. Med. Klinik und Poliklinik, Johannes Gutenberg-Universität Mainz, Federal Republic of Germany.
Kyriatsoulis
A
A
Lohse
A W
AW
Gerken
G
G
Meyer zum Büschenfelde
K H
KH
Manns
M
M
D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D001323:Autoantibodies / Q000276:immunology*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D009418:S100 Proteins / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000941:Antigens; D001323:Autoantibodies; D009418:S100 Proteins; D007633:Keratins