Comparison of three histochemical staining methods for the detection of copper in white perch (Morone americana) with abnormal hepatic copper storage.
A comparison of orcein, rubeanic acid and rhodanine histochemical stains in serial sections of liver from white perch (Morone americana) with abnormal hepatic copper storage is described. Staining reactions correlated well with hepatic copper concentrations although the threshold was the most problematic for rhodanine and rubeanic acid. Orcein was positive at a lower concentration than the two other stains. Hepatocellular granules and macrophage centres increased in size and number with copper concentration and could be related to increased lysosomes, as described for other species.
Bunton
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Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
D000818:Animals; D003300:Copper / Q000378:metabolism*; D005260:Female; D006651:Histocytochemistry / Q000379:methods*; D007202:Indicators and Reagents; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D010078:Oxazines; D010471:Perches; D012235:Rhodamines; D013194:Staining and Labeling; D013854:Thioamides
D007202:Indicators and Reagents; D010078:Oxazines; D012235:Rhodamines; D013854:Thioamides; C002313:PAcein; D003300:Copper; C005728:rubeanic acid