Formalin nociception in the mouse does not lead to increased spinal serotonin turnover.
The mouse formalin test is a model of tonic (continuous), chemical/inflammatory nociception. To test the hypothesis that bulbospinal serotonergic pathways modulate such nociception, whole spinal cords from mice pretreated with probenecid and sacrificed at 15, 30, 45 and 60 min after injection of 5% formalin or 0.9% saline in the hindpaw were assayed by high performance liquid chromatography with electrochemical detection for the serotonin metabolite, 5-hydroxyindoleacetic acid, as an index of turnover. No difference in serotonin turnover was found between formalin and saline groups, indicating that increased spinal serotonin release is not a normal response to formalin nociception in the mouse.
Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA.
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CW
Murray
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Cowan
D000818:Animals; D004195:Disease Models, Animal; D005557:Formaldehyde; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010146:Pain / Q000139:chemically induced / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism* / Q000502:physiology; D013116:Spinal Cord / Q000378:metabolism* / Q000503:physiopathology
D005557:Formaldehyde; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid