The enzymology of apurinic/apyrimidinic endonucleases.
Studies on the enzymology of apurinic/apyrimidinic (AP) endonucleases from procaryotic and eucaryotic organisms are reviewed. Emphasis will be placed on the enzymes from Escherichia coli from which a considerable portion of our knowledge has been derived. Recent studies on similar enzymes from eucaryotes will be discussed as well. In addition, we will discuss the chemical and physical properties of AP sites and review studies on peptides and acridine derivatives which incise DNA at AP sites.
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322.
P W
PW
Doetsch
R P
RP
Cunningham
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004249:DNA Damage; D004260:DNA Repair; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043382:DNA-Formamidopyrimidine Glycosylase; D043224:Deoxyribonuclease (Pyrimidine Dimer); D043223:Deoxyribonuclease IV (Phage T4-Induced); D004706:Endodeoxyribonucleases / Q000502:physiology*; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins; D005090:Exodeoxyribonucleases / Q000502:physiology; D006801:Humans; D051379:Mice; D009097:Multienzyme Complexes / Q000502:physiology; D009699:N-Glycosyl Hydrolases / Q000502:physiology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010954:Plasmacytoma / Q000201:enzymology; D051381:Rats
D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; C032219:UV endonuclease; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D043223:Deoxyribonuclease IV (Phage T4-Induced); C468972:endonuclease IV, E coli; D043224:Deoxyribonuclease (Pyrimidine Dimer); D010727:Phosphoric Diester Hydrolases; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase; C468610:DNA-formamidopyrimidine glycosylase, E coli; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase