A lectin-based monoclonal enzyme immunoassay to distinguish fucosylated and non-fucosylated alpha-fetoprotein molecular variants.
Purified alpha-fetoprotein (AFP), fucosylated AFP mixtures, and 40 sera from patients with AFP-producing hepatocellular carcinoma were analysed by monoclonal enzyme immunoassay (EIA) to distinguish fucosylated and nonfucosylated AFP molecular variants. FUC-AFP-25 was discriminated from FUC-AFP-75 by the EIA using monoclonal antibody 18H4 in the range of total AFP concentrations from 100 to 800 ng/mL. In addition, sera from 40 patients with hepatocellular carcinoma, with AFP concentrations from 100 to 1270 ng/mL and with fucosylated AFP from 0 to 100% by conventional cross immuno-affinoelectrophoresis, were also analysed by the present EIA. A statistically significant correlation was obtained between the data from the present EIA and from the conventional crossed immuno-affinoelectrophoresis in the range of fucosylated AFP more than 20% and the serum concentration of AFP more than 100 ng/mL. These results indicate that the present EIA is useful for clinical detection of hepatocellular carcinoma during the follow-up of patients with chronic liver diseases.
Department of Internal Medicine, Niigata University School of Medicine, Japan.
Y
Y
Suzuki
Y
Y
Aoyagi
M
M
Muramatsu
K
K
Igarashi
A
A
Saito
M
M
Oguro
M
M
Isemura
H
H
Asakura
D000911:Antibodies, Monoclonal; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis; D005643:Fucose / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins; D008113:Liver Neoplasms / Q000175:diagnosis; D037121:Plant Lectins; D011137:Polystyrenes; D012016:Reference Values; D015203:Reproducibility of Results; D000509:alpha-Fetoproteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D037102:Lectins; D037121:Plant Lectins; D011137:Polystyrenes; D000509:alpha-Fetoproteins; C033619:lentil lectin; D005643:Fucose