Caffeine inhibits forskolin-stimulated cyclic AMP accumulation in rat brain.
Caffeine potently inhibited forskolin-stimulated cyclic AMP accumulation in slices of rat cerebral cortex. The IC50 for this inhibition was 21 +/- 2.9 microM, and 200 microM caffeine almost completely blocked the forskolin response. Theophylline which is a behavioural stimulant mimicked the effect of caffeine. However, there was only a slight inhibition of the forskolin response by theobromine and IBMX (3-isobutyl-1-methylxanthine), both of which lack behavioral stimulant effects.
Department of Pharmacology, Emory University Medical School, Atlanta, GA 30322.
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Mante
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Minneman
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002110:Caffeine / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000378:metabolism; D005576:Colforsin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013805:Theobromine / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology
D005576:Colforsin; D002110:Caffeine; D013806:Theophylline; D000242:Cyclic AMP; D013805:Theobromine; D015056:1-Methyl-3-isobutylxanthine