Lymphocyte-fibroblast adhesion. A useful model for analysis of the interaction of the leucocyte integrin LFA-1 with ICAM-1.
The adhesion of human T lymphoblasts to ICAM-1-expressing normal dermal fibroblasts has been assessed as a sensitive model system for the analysis of the interaction of the leucocyte integrin LFA-1 with its counter-receptor ICAM-1. Using this model system, the effects of factors known to regulate the activity of LFA-1 have been quantitated: temperature; concentration of divalent cations; and exposure to phorbol esters. We show here that under the appropriate assay conditions, this model system represents a useful and simple alternative to the detection of leucocyte binding to purified ICAM-1 and also has the additional advantage of permitting more sensitive quantification than is possible using the homotypic adhesion assay.
Cabañas
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Macrophage Laboratory, Imperial Cancer Research Fund, London, UK.
Hogg
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D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000378:metabolism; D002413:Cations, Divalent; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D013696:Temperature
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D002413:Cations, Divalent; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D010703:Phorbol Esters; D018799:Intercellular Adhesion Molecule-1