Effects of body suspension on soleus muscle fibres and spinal motoneurones in the rat.
1. After 14 days of body suspension, fibre type composition and fibre cross-sectional area in the soleus muscle of 17-week-old male Sprague-Dawley rats were investigated. Oxidative enzyme activity of soleus motoneurones in the spinal cord was also examined. 2. After suspension, soleus muscle weight decreased by 44.2%, the cross-sectional area of SO and FOG fibres decreased by 60.4% and 58.6%, respectively. 3. The percentage of fibre types was not changed by suspension. However, ATPase activity after alkaline preincubation was markedly inhibited in FOG fibres. 4. Oxidative enzyme activity of soleus motoneurones was not changed by suspension. 5. This study demonstrates that using mature animals body suspension induces atrophy of fast- and slow-twitch fibres accompanied with the selective inhibition in ATPase activity of fast-twitch fibres, and without changes in histochemical profiles of the corresponding motoneurones.
College of Liberal Arts and Sciences, Kyoto University, Japan.
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S
Taguchi
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H
Morii
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A
Ishihara
D000818:Animals; D006019:Glycolysis; D006112:Gravitation; D006651:Histocytochemistry; D008297:Male; D009046:Motor Neurons / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology / Q000201:enzymology*; D013385:Succinate Dehydrogenase / Q000378:metabolism
D013385:Succinate Dehydrogenase