A new assay for transglutaminase.
A new solid-phase micro assay for transglutaminase has been developed. Casein bound to microtitre plates and biotin-labelled casein were used as substrates in a transglutaminase-dependent cross-linking reaction. The resulting immobilized biotin was visualized by addition of avidin-labelled alkaline phosphatase followed by p-nitrophenyl phosphate. The colour development was monitored at 405 nm. Tissue transglutaminase was used as test enzyme. The method was also applied to normal and Factor XIII-deficient plasma.
Hospital Pharmacy, Malmö General Hospital, Sweden.
B
B
Seiving
P
P
Stenberg
B
B
Nilsson
D000469:Alkaline Phosphatase; D000818:Animals; D001360:Avidin; D001710:Biotin; D002364:Caseins / Q000378:metabolism; D005177:Factor XIII Deficiency / Q000201:enzymology; D006168:Guinea Pigs; D013053:Spectrophotometry; D011503:Transglutaminases / Q000032:analysis* / Q000378:metabolism
D002364:Caseins; D001360:Avidin; D001710:Biotin; D011503:Transglutaminases; D000469:Alkaline Phosphatase