Discontinuous undercondensation of centromeric heterochromatin in mouse chromosomes: evidence in Hoechst 33258-treated cells.
Mouse chromosomes from the L929 cell line have been treated with Hoechst 33258 to induce undercondensation of centromeric heterochromatin. The morphological changes induced by this fluorochrome were analyzed in electron micrographs of whole-mounted chromosomes. Results show that the condensation inhibition of centromeric heterochromatin caused by Hoechst 33258 is not produced homogeneously and suggest compositional differences within an individual centromere.
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
J
J
de la Torre
V
V
Goyanes
J
J
Gosálvez
D000818:Animals; D006690:Bisbenzimidazole / Q000494:pharmacology*; D002460:Cell Line; D002503:Centromere / Q000187:drug effects / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D006570:Heterochromatin / Q000187:drug effects / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron
D006570:Heterochromatin; D006690:Bisbenzimidazole