Differential effect of sunflower seed oil on hepatic and renal D-amino acid oxidase in the rat.
1. The specific activity of hepatic and renal peroxisomal D-amino acid oxidase (D-AAOX) was measured in rats fed diets containing various quantities of vegetable oil. 2. Increasing the amount of dietary sunflower seed oil (SSO) from 10 to 25% (w/w) reduced the specific activity of hepatic D-AAOX by up to 30% after 10 days. 3. In both tissues, the enzyme activity was moderately decreased during the first two-day period after administration of the 25% SSO diet was begun. Unlike hepatic D-AAOX, renal D-AAOX returned to its baseline level in the kidney after the third day. 4. In contrast to SSO, hydrogenated coconut oil (HCO) did not evoke alterations of D-AAOX activity. 5. The activity levels of another peroxisomal enzyme, L-2-hydroxy acid oxidase (L-HAOX), in the liver of rats fed the high-SSO diet vs those fed the control diet were similar. 6. The subcellular distribution of D-AAOX and L-HAOX was not altered in the liver of rats fed the 25% SSO diet during the 10-day period.
Centre de Biochimie et de Biologie Moléculaire du CNRS, Marseille, France.
P
P
Brachet
S
S
Carreira
A
A
Puigserver
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D000074263:Coconut Oil; D003605:D-Amino-Acid Oxidase / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism*; D005224:Fats, Unsaturated / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010938:Plant Oils / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000074242:Sunflower Oil; D013997:Time Factors
D004041:Dietary Fats; D005224:Fats, Unsaturated; D010938:Plant Oils; D000074242:Sunflower Oil; D000429:Alcohol Oxidoreductases; C021380:L-2-hydroxyacid oxidase; D003605:D-Amino-Acid Oxidase; D000074263:Coconut Oil