Regulation by retinoic acid of ICAM-1 expression on human tumor cell lines.
In a group of four human tumor cell lines comprising one melanoma, one glioma, one teratocarcinoma and one neuroblastoma, the expression of the intercellular adhesion molecule-1 (ICAM-1) was found to be significantly increased following treatment with 10 microM of all-trans retinoic acid. In the melanoma and glioma cell lines HS 294T and HS 683, greater than 90% of the cells reacted with the anti-ICAM-1 monoclonal antibody (mAb) CL203.4 in the absence of treatment. Retinoic acid increased the cell surface expression of the molecule by 2-fold. In the teratocarcinoma and neuroblastoma cell lines, TERA-2 and SK-N-SH, the constitutive expression of ICAM-1 was weak, the percentage of cells stained above the background being less than 25%. Retinoic acid induced ICAM-1 expression in greater than 80% of the cells and increased the levels of expression by 2.5 to 3-fold. Immunoprecipitation studies in biosynthetically labeled cells as well as RNase protection analysis confirmed that retinoic acid treatment increased the amount of ICAM-1 at both the protein and mRNA level. The induction or stimulation occurred within 24 h, was maximal after 4 days and reversible.
CHUL Research Center, Faculty of Medicine, Laval University, Quebec City, Canada.
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Bouillon
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Tessier
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Boulianne
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Destrempe
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Audette
D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008856:Microscopy, Fluorescence; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins; D012260:Ribonucleases / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D012333:RNA, Messenger; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D014212:Tretinoin; D007371:Interferon-gamma; D012260:Ribonucleases