Reaction of dipeptidylpeptidase IV with substrate-analogous azapeptides.
The reaction of dipeptidyl peptidase IV (EC 3.4.14.5.) with azapeptide substrates containing azaalanine or azaproline in the P1-position was investigated. Accumulation of a fairly stable acyl-enzyme could be shown for ester substrates. Ala-AzaPro-pNA is a very poor substrate of DP IV and does not accumulate an acyl-enzyme. DP IV does not react with active-site titrants for trypsin-like serine proteases.
Institute of Pharmacology and Toxicology, Medical Academy Erfurt, GDR.
U
U
Neumann
T
T
Steinmetzer
A
A
Barth
H U
HU
Demuth
D000595:Amino Acid Sequence; D000818:Animals; D001372:Aza Compounds / Q000138:chemical synthesis / Q000494:pharmacology*; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007202:Indicators and Reagents; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology*; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D001372:Aza Compounds; D007202:Indicators and Reagents; D009842:Oligopeptides; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4