Isolation of genomic sequence encoding a biologically active bovine TGF-alpha protein.
Genomic clones encoding bovine TGF-alpha were identified by hybridization with probes derived from human TGF-alpha sequence. Nucleotide sequence of the clones predicts that mature bovine TGF-alpha is a 50 amino acid polypeptide which shares 96% and 92% homology with human and rat TGF-alpha, respectively. Bovine TGF-alpha with the predicted sequence was chemically synthesized and tested for activity. Synthetic bovine TGF-alpha competes in a radioreceptor assay with labelled mouse EGF with activity parallel to that of human TGF-alpha and mouse EGF. The mitogenic activity of bovine TGF-alpha is comparable to that of human EGF in causing proliferation of bovine mammary epithelial cells. An approximately 5.0 kilobase RNA transcript is observed in polyadenylated RNA from MDBK cells by Northern blot analysis. The polymerase chain reaction detects the presence of a TGF-alpha transcript in many bovine tissues. These data indicate that bovine TGF-alpha may be a normal regulator of cell growth in the bovine animal.
Zurfluh
L L
LL
Biological Sciences Division, Monsanto Company, St. Louis, Missouri 63198.
Bolten
S L
SL
Byatt
J C
JC
McGrath
M F
MF
Tou
J S
JS
Zupec
M E
ME
Krivi
G G
GG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D066246:ErbB Receptors / Q000378:metabolism; D005091:Exons; D005260:Female; D015698:Genomic Library; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011869:Radioligand Assay; D051381:Rats; D015183:Restriction Mapping; D012469:Salivary Glands / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D016211:Transforming Growth Factor alpha / Q000235:genetics* / Q000378:metabolism
D015345:Oligonucleotide Probes; D016211:Transforming Growth Factor alpha; D012313:RNA; D004247:DNA; D066246:ErbB Receptors