Wegener autoantigen and myeloblastin are encoded by a single mRNA.
Myeloblastin is a serine protease that has been identified in the human leukemia cell line HL-60. Down-regulation of this protease can inhibit proliferation and induce differentiation of promyelocyte-like human leukemic cells. Proteinase 3, a serine protease of human neutrophils, has been identified as the Wegener autoantigen. A high level of homology between myeloblastin and proteinase 3 has suggested that they may be a single serine protease. We have recently completed the 5'-terminal nucleotide sequence of proteinase 3 and shown that its mRNA was also expressed in HL-60 cells and in cells from patients with acute myeloid leukemia. Here we demonstrate that myeloblastin and proteinase 3 are encoded by a single mRNA.
Labbaye
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C
Department of Physiology and Cellular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Musette
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P
Cayre
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YE
D001324:Autoantigens / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007938:Leukemia; D008969:Molecular Sequence Data; D053146:Myeloblastin; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D015183:Restriction Mapping; D012697:Serine Endopeptidases / Q000235:genetics*
D001324:Autoantigens; D015342:DNA Probes; D004273:DNA, Neoplasm; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D011061:Poly A; D012697:Serine Endopeptidases; D053146:Myeloblastin