Lack of correlation between immunoglobulin and T cell receptor gene rearrangements and TdT expression in acute myeloid leukaemia.
Thirty-two cases of acute myeloid leukaemia (AML) were examined for expression of terminal deoxynucleotidyl transferase (TdT) and rearrangements of the genes coding for the immunoglobulin heavy chain and the beta chain of the T cell receptor, in order to establish whether these two forms of lineage infidelity are linked. In 17 cases of AML with greater than or equal to 10% TdT+ cells, three cases showed evidence of gene rearrangement, two having clonal rearrangements in the immunoglobulin gene and one with a rearranged T cell receptor gene. Among 15 AML cases without significant numbers of TdT-positive blasts, three cases had rearrangements in both immunoglobulin and T cell receptor genes, while a fourth case had an immunoglobulin gene rearrangement. No relationship was seen between lymphoid gene rearrangements and expression of the lymphoid surface antigens CD7 and CD10. The lack of association between TdT expression and gene rearrangements does not support the concept of an orderly activation of the recombinase machinery in those cases of AML with features of early lymphoid differentiation.
Department of Haematology, Westmead Hospital, N.S.W., Australia.
G
G
Davey
K F
KF
Bradstock
R F
RF
Kefford
Y
Y
Wishart
A
A
Kabral
P
P
Grimsley
W G
WG
Hughes
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019011:Antigens, CD7; D000943:Antigens, Differentiation / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D015139:Blotting, Southern; D002648:Child; D002675:Child, Preschool; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D015321:Gene Rearrangement; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D005803:Genes, Immunoglobulin; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015260:Neprilysin
D019011:Antigens, CD7; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D004253:DNA Nucleotidylexotransferase; D015260:Neprilysin