Effects of dextran sulphate on renal dysfunctions induced by gentamicin as determined by the kidney perfusion technique in rats.
The effect of dextran sulphate on accumulation of gentamicin within the renal cortex and on renal functional changes induced by gentamicin has been investigated using the rat renal perfusion technique. Gentamicin accumulation decreased by the coadministration of dextran sulphate, the degree being proportional to dextran sulphate concentration. The filtered load of gentamicin was unaffected by dextran sulphate. The perfusion of kidney with gentamicin brought about a decrease in the reabsorption of Na+ and an increase in the rate of urine flow with no effect on that of glomerular filtration. The addition of dextran sulphate in the perfusate resulted in the resumption of these levels to those of the control. Dextran sulphate thus apparently interacts with gentamicin in the lumen of proximal tubules to inhibit the reabsorption of gentamicin by proximal tubular cells, and prevents the renal dysfunctions induced by gentamicin.
Tokyo College of Pharmacy, Japan.
S
S
Kikuchi
Y
Y
Aramaki
H
H
Nonaka
S
S
Tsuchiya
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D016264:Dextran Sulfate / Q000494:pharmacology*; D005839:Gentamicins; D005919:Glomerular Filtration Rate; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced / Q000517:prevention & control*; D007677:Kidney Function Tests; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D005839:Gentamicins; D016264:Dextran Sulfate