Chromosomal aberrations in patients with primary biliary cirrhosis.
Chromosomal aberrations in untreated lymphocyte cultures, bleomycin (BLM)-induced aberrations and sister chromatid exchanges (SCE) in the peripheral blood lymphocytes of 11 patients suffering from primary biliary cirrhosis (PBC) and 14 matched control individuals were analysed. The lymphocytes of the PBC patients had on average a lower mitotic index (2.3) compared with controls (3.5) in the untreated cultures. The mean baseline rate of aberrations of the cultured lymphocytes of the patients was 5.3 aberrations per 100 metaphases (%); this was significantly different (P = 0.0291) from that of the controls with a mean of 2.3%. In lymphocytes of the patients and controls, most of the aberrations observed took the form of gaps; there was an almost equal breakage rate in both groups (0.5% and 0.4%, respectively). The average number of mitoses with aberrations in the PBC patients studied was double that of the controls (4.9% and 2.3% respectively, P = 0.0323). The mean number of the BLM-induced aberrations was 54.0% and 27.7% for the lymphocytes of the patients and controls, respectively. The mean number of the aberrant mitoses in the BLM cultures was 6 times higher than that of the untreated cultures for both groups, 25.7% and 14.6% respectively (P = 0.018). The chromosomal distribution of baseline and induced aberrations was not random. The PBC patients had a mean number of 8.7 SCE per mitosis, which was significantly higher than the SCEs in the controls (6.3 SCE per mitosis; P = 0.0156). The evidence suggests that the chromosomes of the lymphocytes of PBC patients may be less stable than those of the control individuals in this study.
Institut für Rechtsmedizin der Universität, Mainz, Federal Republic of Germany.
A
A
Notghi
U
U
Nestle
G
G
Rittner
P
P
Brissot
H
H
Jouanolle
M
M
Manns
E
E
Schleiermacher
C
C
Rittner
D000328:Adult; D000368:Aged; D001761:Bleomycin / Q000494:pharmacology; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007621:Karyotyping; D008105:Liver Cirrhosis, Biliary / Q000235:genetics*; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008875:Middle Aged; D008940:Mitotic Index / Q000187:drug effects; D012854:Sister Chromatid Exchange / Q000187:drug effects
D001761:Bleomycin