CD11/CD18 cell surface adhesion glycoproteins: discordance of monocyte function and expression in response to stimulation.
The adherence of blood leukocytes to vascular endothelium precedes their diapedesis into the extravascular space. These processes require the expression of adherence glycoproteins on the cell surface of the leukocyte. The relative importance of these adherence molecules is so far poorly understood. However, there is evidence to suggest that a disparity exists between the surface receptor expression of these glycoproteins and leukocyte adherence to vascular endothelial cells in culture. We have investigated the importance of each of the adhesion glycoproteins CD11a, CD11b, and CD11c in mediating the adherence of human monocytes to endothelial cells in culture. We have also investigated the chronological relationship between changes in monocyte adherence to endothelial cells and the surface expression of CD11a, CD11b, and CD11c following stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLP). The increase in adherence occurred within 1 minute, but declined if monocytes were preincubated with fMLP for up to 30 minutes. The surface expression of adherence molecules demonstrated a significant increase in CD11a and CD11b in the presence of fMLP after 10 min and was maintained while monocyte adherence to endothelium declined. These changes in surface receptor expression were quantitated using an immunolabeling technique. It is suggested that fMLP stimulation of monocyte adherence is unlikely to be solely dependent on increased surface receptor expression of adhesion molecules.
Wellcome Research Laboratories, Beckenham, Kent.
D G
DG
Hassall
P M
PM
Bath
A M
AM
Gladwin
J E
JE
Beesley
D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000502:physiology*; D001011:Aorta; D018845:CD11 Antigens; D018821:CD18 Antigens; D002448:Cell Adhesion; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002478:Cells, Cultured; D004705:Endocytosis; D004730:Endothelium, Vascular / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000502:physiology*; D016253:Microscopy, Immunoelectron; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D013552:Swine
D015703:Antigens, CD; D018845:CD11 Antigens; D018821:CD18 Antigens; D009240:N-Formylmethionine Leucyl-Phenylalanine