A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus.
A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
K
K
Muraiso
M
M
Hijikata
S
S
Ohkoshi
M J
MJ
Cho
M
M
Kikuchi
N
N
Kato
K
K
Shimotohno
D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis*; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D015678:Viral Structural Proteins / Q000032:analysis / Q000235:genetics*
D015345:Oligonucleotide Probes; D012367:RNA, Viral; D015678:Viral Structural Proteins; D012194:RNA-Directed DNA Polymerase