Detection of lipopolysaccharides by ethidium bromide staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
A rapid and easy method for staining lipopolysaccharides with ethidium bromide is described. Lipopolysaccharides could be visualized by ethidium bromide with almost the same sensitivity as found with the silver-staining method in less than 30 min. The ethidium bromide-staining method was particularly suitable for staining lipopolysaccharides possessing acidic O-specific polysaccharides, which were poorly visualized by silver staining.
Department of Bacteriology, Nagoya University School of Medicine, Aichi, Japan.
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N
Kido
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M
Ohta
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Kato
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000276:immunology; D004996:Ethidium; D008070:Lipopolysaccharides / Q000032:analysis* / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000276:immunology; D012760:Shigella / Q000276:immunology; D013194:Staining and Labeling
D008070:Lipopolysaccharides; D004996:Ethidium