Evidence for involvement of lymphocyte function-associated antigen 1 in T cell migration to epidermis.
Although it is well known that in various T cell-mediated skin diseases T cells migrate preferentially to epidermis, no direct evidence has been presented in which molecules on T cells are important in directing T cell traffic to epidermis. We have previously established CD4+ autoreactive cloned T cells with a special tropism for epidermis in vitro as well as in vivo. Antibody inhibition studies demonstrated that only anti-lymphocyte function associated Ag 1 (anti-LFA-1) mAb completely inhibited the in vitro migration of the T cells toward the epidermis, whereas mAb against other T cell surface molecules had little or no effect. Monovalent F(ab) fragment of the anti-LFA-1 mAb, although less efficient, also inhibited the T cell migration. The apparent dependency of the inhibition on the anti-alpha-chain mAb suggested a major role for the alpha-chain of LFA-1 in T cell migration to epidermis. The relevance of an LFA-1-dependent mechanism to the epidermotropic migration of T cells was further strengthened by the findings that the T cell migration to epidermis was inhibited by divalent cation depletion, cytochalasin B, and low temperature. These findings indicate that the LFA-1 molecule, which is thought to be primarily involved in cell-to-cell adhesions, also plays an important role in directing T cell migration to epidermis.
Department of Dermatology, Kyorin University School of Medicine, Tokyo, Japan.
T
T
Shiohara
N
N
Moriya
K
K
Saizawa
C
C
Gotoh
H
H
Yagita
M
M
Nagashima
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002118:Calcium / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000502:physiology; D004817:Epidermis / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D011487:Protein Conformation; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; D000939:Epitopes; D016169:Lymphocyte Function-Associated Antigen-1; D003571:Cytochalasin B; D008274:Magnesium; D002118:Calcium