[Mechanism of interaction of DNA with fluorescent dye Hoechst 33258].
From the study of absorption and fluorescence spectra of the complex "Hoechst-33258"--DNA at different pH it is shown that AT--specific complex with DNA is formed by the neutral dye molecule, whereas the cationic state of the dye molecule forms the nonspecific complex. Possible formation of a specific complex in which the dye is bound to DNA in its major groove is discussed.
V N
VN
Umetskaia
Iu M
IuM
Rozanov
D006690:Bisbenzimidazole / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D011522:Protons; D013050:Spectrometry, Fluorescence
D011522:Protons; D004247:DNA; D006690:Bisbenzimidazole
O mekhanizme vzaimodeĭstviia DNK s fluorestsentnym krasitelem Khekhst 33258.