Phloretin and citrate promote the differentiation rate from epimastigote to metacyclic forms of Trypanosoma cruzi.
We have investigated the effects of the metabolic inhibitors, phloretin, 2-deoxy-D-glucose, maleate and trans-aconitate, as well as two intermediates of the tricarboxylic-acid cycle, acetate and citrate, on the growth and metacyclogenesis of Trypanosoma cruzi. 0.1 mM phloretin increased the percentage of metacyclic forms about 2.7-fold without affecting growth rate, whereas the other inhibitors had no apparent effect on either growth or differentiation rates. The addition of 5 mM citrate stimulated differentiation by about 2.6-fold. When either 10 mM citrate or 10 mM acetate were added, on the other hand, both the growth and differentiation rates were severely inhibited.
Adroher
F J
FJ
Department of Parasitology, Faculty of Pharmacy, University of Granada, Granada, Spain.
Osuna
A
A
Lupiáñez
J A
JA
D000085:Acetates / Q000494:pharmacology; D000156:Aconitic Acid / Q000494:pharmacology; D000818:Animals; D002951:Citrates / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology; D008298:Maleates / Q000494:pharmacology; D010693:Phloretin / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development
D000085:Acetates; D002951:Citrates; D008298:Maleates; C030272:maleic acid; D000156:Aconitic Acid; D003847:Deoxyglucose; D010693:Phloretin