Effect of hyperthermia and lipid peroxidation on the erythrocyte membrane structure.
The hyperthermic exposure (39-49 degrees C) of human erythrocyte membranes augmented their lipid peroxidation stimulated by 0.1 mM FeCl3 + 1.5 mM ascorbate while having no significant influence on the non-stimulated lipid peroxidation. No effect of hyperthermia and lipid peroxidation on the post-exposure fluidity of the erythrocyte membrane lipids was found by the fluorescence anisotropy of hexatriene and trimethylaminophenylhexatriene, and excimerization efficiency of pyrene. Exposure to iron/ascorbate increased the accessibility of membrane protein tryptophan residues to acrylamide as judged by fluorescence quenching. These results suggest a higher sensitivity of membrane protein organization than of membrane lipid fluidity to the effect of the system inducing lipid peroxidation.
Department of Biophysics, University of Lódź, Poland.
W
W
Leyko
D
D
Ertel
G
G
Bartosz
D001205:Ascorbic Acid / Q000494:pharmacology; D002712:Chlorides; D004910:Erythrocyte Membrane / Q000502:physiology* / Q000648:ultrastructure; D005290:Ferric Compounds / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology*; D008560:Membrane Fluidity / Q000187:drug effects; D013268:Stimulation, Chemical
D002712:Chlorides; D005290:Ferric Compounds; D001205:Ascorbic Acid; C024555:ferric chloride