Characterization of monoclonal antibodies against the p17 core protein of the human immunodeficiency virus 1.
Five mouse hybridomas which produce monoclonal antibodies against the p17 core protein of HIV-1 have been isolated. Cross-competition assays and mapping with synthetic peptides demonstrate that two closely related epitopes are identified by these antibodies. Directed against two neighbouring peptides at the carboxy-terminal end of the molecule, they can be used for the selective detection of p17 polypeptide in a viral extract or in an infected cell lysate by a solid-phase sandwich enzyme immunoassay.
Cogniaux
J
J
Institut Pasteur du Brabant, Brussels, Belgium.
De Schepper
N
N
Blondiau
M L
ML
Cornet
B
B
Horal
P
P
Vahlne
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D015488:HIV Antigens / Q000032:analysis* / Q000138:chemical synthesis / Q000276:immunology; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis* / Q000138:chemical synthesis / Q000276:immunology; D011233:Precipitin Tests / Q000379:methods; D054301:gag Gene Products, Human Immunodeficiency Virus
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; C052407:HGP-30 peptide, Human immunodeficiency virus-1; D015488:HIV Antigens; D010455:Peptides; D054301:gag Gene Products, Human Immunodeficiency Virus