Action of a new cholinergic agonist, aclatonium napadisilate, on isolated rat pancreatic acini.
The effect of aclatonium napadisilate, a newly synthesized choline ester, on pancreatic exocrine function was compared with that of the muscarinic agonist carbamylcholine in isolated rat pancreatic acini. Both compounds increased amylase release and 45Ca2+ efflux in a dose-dependent fashion, and similarly decreased the binding of [N-methyl-3H]scopolamine to isolated rat pancreatic acini. While aclatonium napadisilate was 20-30 times less potent than carbamylcholine in stimulations of amylase release and 45Ca2+ efflux, the potency of aclatonium napadisilate in inhibiting [N-methyl-3H]scopolamine binding was nearly the same as that of carbamylcholine. These results indicate that aclatonium napadisilate stimulates pancreatic exocrine secretion via muscarinic receptors and Ca2+ mobilization, and its intrinsic activity is less than carbamylcholine in the isolated rat pancreatic acini. Since aclatonium napadisilate is known to increase motility and peristalsis of the gastrointestinal tract, stimulatory effects of aclatonium napadisilate, shown in the present study, on digestive enzyme secretion from the pancreas may provide additional benefit of aclatonium napadisilate in the treatment of various gastrointestinal disorders.
Second Department of Internal Medicine, Kobe University School of Medicine, Japan.
M
M
Fujii
Y
Y
Okabayashi
T
T
Nakamura
S
S
Tani
T
T
Fujisawa
M
M
Otsuki
D000109:Acetylcholine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000681:Amylases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D002217:Carbachol / Q000494:pharmacology; D008297:Male; D019832:N-Methylscopolamine; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D010277:Parasympathomimetics / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology; D012602:Scopolamine Derivatives / Q000378:metabolism
D002132:Calcium Radioisotopes; D010277:Parasympathomimetics; D011976:Receptors, Muscarinic; D012602:Scopolamine Derivatives; C020850:TM 723; D002217:Carbachol; D000681:Amylases; D000109:Acetylcholine; D002118:Calcium; D019832:N-Methylscopolamine