[Studies on defense effects of recombinant human granulocyte colony-stimulating factor (G-CSF) to infections. II. Priming effect for superoxide production by human neutrophil].
In present study, we have investigated superoxide (O2-) production from human neutrophils by recombinant human granulocyte colony-stimulating factor (G-CSF) using the microtiter plate for the purpose of being close to the inflammatory site. G-CSF by itself did not induce the release of O2- in human neutrophil on either Fetal Bovine Serum (FBS)-coated plate or plate uncoated with FBS, even if neutrophils were exposed for maximum 3 hr. However, the optimal concentration of G-CSF (50 ng/ml) was able to prime human neutrophils with enhance of O2- release stimulated by the chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine (FMLP) from 10(-6) to 10(-8) M, but not by the non chemoattractant such as phorbol myristate acetate (PMA), concanavalin A, and ionomycin. These findings indicate that G-CSF might enhance bactericidal activity of neutrophils by priming them penetrating into the inflammatory site.
Second Department of Internal Medicine, Nagasaki University School of Medicine.
J
J
Kadota
M
M
Hirota
K
K
Tomono
R
R
Senju
K
K
Fukushima
Y
Y
Dotsu
K
K
Komori
S
S
Kohno
K
K
Yamaguchi
K
K
Hara
D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology; D013268:Stimulation, Chemical; D013481:Superoxides / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D013481:Superoxides; D016179:Granulocyte Colony-Stimulating Factor; D009240:N-Formylmethionine Leucyl-Phenylalanine