Detection of a novel fimbrial structure on the surface of Salmonella enteritidis by using a monoclonal antibody.
A fimbrialike structure expressed on the surface of Salmonella enteritidis was identified by using a monoclonal antibody (69/25) produced against intact S. enteritidis cells. Fimbriae were less than 5 nm in diameter and carried a protein consisting of subunits with a molecular weight of 14,300. No hemagglutinating activity associated with the fimbriae was detected. An epitope on the fimbrial antigen identified by monoclonal antibody 69/25 was expressed by all 58 S. enteritidis strains, 12 of 36 S. dublin strains, and a single strain of S. moscow examined. None of 169 other isolates tested from 17 salmonella serogroups expressed this epitope.
Thorns
C J
CJ
Central Veterinary Laboratory, Ministry of Agriculture, Fisheries and Food, Weybridge, Surrey, United Kingdom.
Sojka
M G
MG
Chasey
D
D
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D000939:Epitopes; D010861:Fimbriae, Bacterial / Q000276:immunology / Q000648:ultrastructure*; D006386:Hemagglutination Tests; D008854:Microscopy, Electron; D012477:Salmonella enteritidis / Q000145:classification / Q000276:immunology / Q000648:ultrastructure*; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D000939:Epitopes