Phosphorylation of recoverin, the calcium-sensitive activator of photoreceptor guanylyl cyclase.
Recoverin, a new calcium binding protein from bovine rod photoreceptor cells, activates guanylyl cyclase below a free calcium concentration of 200 nM. We show here that recoverin is phosphorylated by an endogenous kinase and Mg-ATP at the same decreased calcium concentration. The calcium-dependent activation of guanylyl cyclase is enhanced in the presence of ATP. We suggest that phosphorylation of recoverin reinforces the stimulation of guanylyl cyclase at decreased calcium concentrations.
Lambrecht
H G
HG
Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, Germany.
Koch
K W
KW
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000378:metabolism* / Q000494:pharmacology; D002417:Cattle; D004789:Enzyme Activation / Q000187:drug effects; D005136:Eye Proteins; D006162:Guanylate Cyclase / Q000378:metabolism*; D051597:Hippocalcin; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D010766:Phosphorylation; D010786:Photoreceptor Cells / Q000201:enzymology*; D011494:Protein Kinases / Q000378:metabolism; D051603:Recoverin; D012374:Rod Cell Outer Segment / Q000737:chemistry
D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D051603:Recoverin; D051597:Hippocalcin; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D006162:Guanylate Cyclase; D002118:Calcium