Predicted structure of rabbit N-terminal, calcitonin and katacalcin peptides.
Poly A rich RNA was extracted from rabbit thyroid and cDNA obtained by the action of reverse transcriptase. The cDNA was used to construct a library in lambda GT 11. Screening of the library with a radio-labelled probe specific for human calcitonin allowed the isolation of a clone containing an open reading frame with a high homology with human and murine exon 4 of calcitonin/calcitonin gene-related peptide gene. This sequence codes for a typical calcitonin precursor. We deduced the amino acid sequence of rabbit N-terminal peptide, calcitonin and katacalcin.
U 113 INSERM CNRS, CHU St. Antoine, Paris, France.
K
K
Martial
S
S
Minvielle
A
A
Jullienne
N
N
Segond
G
G
Milhaud
F
F
Lasmoles
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002116:Calcitonin / Q000737:chemistry / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics*; D011061:Poly A / Q000235:genetics* / Q000302:isolation & purification; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D011817:Rabbits; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013961:Thyroid Gland / Q000378:metabolism
D010446:Peptide Fragments; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; C037553:katacalcin; D002116:Calcitonin; D004247:DNA; D012194:RNA-Directed DNA Polymerase