Human cortical neuronal cell line: establishment from a patient with unilateral megalencephaly.
A cell line has been established in continuous culture of human cerebral cortical neurons obtained from a patient with unilateral megalencephaly, a disorder associated with continued proliferation of immature neuronal cells. When differentiated in the presence of nerve growth factor, 1-isobutyl-3-methylxanthine, and dibutyryl adenosine 3',5'-monophosphate (cAMP), the cells display mature neuronal morphology with numerous long, extensively branched processes with spines and varicosities. The cells stain positively for neurofilament protein and neuron-specific enolase (selective neuronal markers) but are negative for glial markers, such as glial fibrillary acidic protein, S-100, and myelin basic protein. The cells also stain positively for the neurotransmitters gamma-aminobutyric acid (GABA), glutamate, somatostatin, cholecystokinin-8, and vasoactive intestinal polypeptide. These cells may facilitate characterization of neurons in the human central nervous system.
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
G V
GV
Ronnett
L D
LD
Hester
J S
JS
Nye
K
K
Connors
S H
SH
Snyder
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D001927:Brain Diseases / Q000473:pathology*; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002540:Cerebral Cortex / Q000473:pathology*; D046508:Culture Techniques / Q000379:methods; D005260:Female; D006801:Humans; D007223:Infant; D009414:Nerve Growth Factors / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D018377:Neurotransmitter Agents / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D005680:gamma-Aminobutyric Acid; D003994:Bucladesine; D015056:1-Methyl-3-isobutylxanthine