The mitosis-specific monoclonal antibody MPM-2 recognizes phosphoproteins associated with the nuclear envelope in Chlamydomonas reinhardtii cells.
The monoclonal antibody MPM-2 recognizes a family of phosphorylated proteins present in mitotic cells. In a number of organisms it stains nuclei and also cytoskeletal structures which contain or organize tubulin. In mitotic Chlamydomonas reinhardtii cells MPM-2 reacts with phosphoproteins associated with the nuclear envelope (NE). Staining of the NE region appears in preprophase, reaches a maximum intensity in metaphase/anaphase and disappears rapidly in telophase. Localized hyperphosphorylation of the anterior NE region is apparent in many cells throughout mitosis. The distribution and timing of MPM-2 labeling suggests that in Chlamydomonas MPM-2 may be interacting with lamin-like phosphoproteins.
Research School of Biological Sciences, Australian National University, Canberra.
J D
JD
Harper
P N
PN
Rao
P C
PC
John
D000911:Antibodies, Monoclonal; D002453:Cell Cycle / Q000502:physiology; D002696:Chlamydomonas / Q000032:analysis* / Q000166:cytology; D003599:Cytoskeleton / Q000502:physiology; D003902:Detergents; D000939:Epitopes / Q000032:analysis; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000502:physiology*; D009685:Nuclear Envelope / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D003902:Detergents; D000939:Epitopes; D009687:Nuclear Proteins; D010750:Phosphoproteins