Interferon production by cultured human trophoblast induced with double stranded polyribonucleotide.
Human trophoblast cultures were established from term placentae. Upon exposure to polyriboinosinic-polyribocytidylic acid (poly(I:C] the cultures produced interferon. Results of neutralization experiments and indirect immunofluorescence studies indicated that the trophoblast produces beta-interferon. The fusion of trophoblast cells into syncytia was accompanied by a restriction in interferon release after stimulation with poly(I:C). It was also demonstrated that the malignant choriocarcinoma cell line JAR produced less interferon than the non-transformed cytoorsyncytiotrophoblast.
Department of Virus and Cancer, Danish Cancer Society, Aarhus C.
F D
FD
Toth
C
C
Juhl
N
N
Nørskov-Lauritsen
P
P
Mosborg Petersen
P
P
Ebbesen
D002469:Cell Separation; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000096:biosynthesis*; D007633:Keratins / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D011070:Poly I-C / Q000494:pharmacology*; D013997:Time Factors; D014327:Trophoblasts / Q000276:immunology / Q000378:metabolism*
D007370:Interferon Type I; D007633:Keratins; D007371:Interferon-gamma; D011070:Poly I-C