Loss of 4.5S RNA induces the heat shock response and lambda prophage in Escherichia coli.
During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of protein synthesis uniquely induces the heat shock response. Several hours later, lambda prophage was induced and the cells lysed.
Bourgaize
D B
DB
Department of Biochemistry, University of Massachusetts, Amherst 01003.
Phillips
T A
TA
VanBogelen
R A
RA
Jones
P G
PG
Neidhardt
F C
FC
Fournier
M J
MJ
D010582:Bacteriophage lambda / Q000187:drug effects / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006358:Hot Temperature; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D007700:Kinetics; D008242:Lysogeny; D008715:Methionine / Q000378:metabolism; D012329:RNA, Bacterial / Q000502:physiology*
D006360:Heat-Shock Proteins; D012329:RNA, Bacterial; D007544:Isopropyl Thiogalactoside; D008715:Methionine