Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification.
Two human cell lines (UACC-812 and 893), both containing significant amplification of the HER-2/neu gene, were established from biopsy specimens of breast carcinomas. One patient had Stage II breast carcinoma; the other had metastatic disease. Characterisation of these lines has revealed that both are highly aneuploid containing multiple clonal chromosome alterations, have doubling times near 100 h, and are oestrogen and progesterone receptor negative. Electron microscopy demonstrates that both lines contain numerous microvilli, cytoplasmic filaments, multivesicular bodies, and desmosomes. Immunoblot analysis for P-glycoprotein using the monoclonal antibody C219 was negative for both patient cell lines. These relatively rare cell lines may represent a useful model to investigate human breast carcinomas.
Meltzer
P
P
University of Michigan Cancer Center, Department of Pediatrics, Ann Arbor 48109-0668.
Leibovitz
A
A
Dalton
W
W
Villar
H
H
Kute
T
T
Davis
J
J
Nagle
R
R
Trent
J
J
D000328:Adult; D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics* / Q000473:pathology; D002871:Chromosome Banding; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007621:Karyotyping; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D018719:Erb-b2 Receptor Tyrosine Kinases; D014407:Tumor Cells, Cultured / Q000473:pathology
D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases