Monoclonal antibodies to lipocortin-1 as probes for biological function.
We have developed two monoclonal antibodies to human lipocortin-1 (103 and 105) as reagents for quantitating the protein in biological systems and neutralizing its activity. Lipo 105 is a high affinity antibody that is functional in ELISA and Western blot formats. The antibody recognizes a site between amino acids 30 and 55 in the lipocortin-1 sequence and can be used on native or denatured protein. Lipo 103 is an antibody that neutralizes the phospholipase A2 inhibitory activity of lipocortin-1 by blocking binding of the protein to phospholipid surfaces. The antibody is specific for native human lipocortin-1. Lipo 103 was recently shown to block lipocortin-1-dependent differentiation of a squamous carcinoma cell line, demonstrating its usefulness as a probe for function.
Pepinsky
R B
RB
Biogen Inc., Cambridge, MA 02142.
Sinclair
L K
LK
Dougas
I
I
Liang
C M
CM
Lawton
P
P
Browning
J L
JL
D000818:Animals; D017302:Annexins; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000276:immunology* / Q000494:pharmacology; D003488:Cyanogen Bromide; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology; D010449:Peptide Mapping; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D011994:Recombinant Proteins
D017302:Annexins; D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D000939:Epitopes; D007202:Indicators and Reagents; D010446:Peptide Fragments; D011994:Recombinant Proteins; D010741:Phospholipases A; D054467:Phospholipases A2; D003488:Cyanogen Bromide