Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells.
We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development.
Molecular Embryology Group, NCI-Frederick Cancer Research and Development Center, Maryland 21702-1201.
B R
BR
Stanton
S W
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Reid
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Parada
D000483:Alleles; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D004622:Embryo, Mammalian; D016259:Genes, myc; D051379:Mice; D016254:Mutagenesis, Insertional; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D011995:Recombination, Genetic; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA