Nature of the inhibitory effect of complex saccharide moieties on the tight binding of human spermatozoa to the human zona pellucida.
Fucoidin and heparin sulfate inhibit binding of human sperm to the human zona pellucida under hemizona assay (HZA) conditions. Here we used the HZA to further assess tight sperm binding with/without preincubation of the sperm with other sulfated and nonsulfated glycoconjugates and charged polymers. Fucoidin significantly inhibited binding compared with controls (greater than 75% inhibition), even if sperm were washed after preincubation with the saccharide. Dextran sulfate also produced significant inhibition, although to a lesser extent (54% inhibition). Chondroitin sulfates A and B, heparin, and dextran did not affect binding. Sodium sulfate and polyglutamic acid did not affect HZA results; polyphosphates produced only moderate inhibition. The potent inhibitory effect of the sulfated carbohydrates fucoidin and dextran is probably competitive (receptor-ligand type) in nature. However, the lack of significant effects of simple charged molecules (nonspecific effects) suggests that the degree of sulfation (charge) may not be crucial to its inhibitory action.
Howard and Georgeanna Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk.
S
S
Oehninger
G F
GF
Clark
A A
AA
Acosta
G D
GD
Hodgen
D000328:Adult; D002809:Chondroitin Sulfates / Q000494:pharmacology; D016264:Dextran Sulfate / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology; D008297:Male; D009865:Oocytes / Q000502:physiology; D011134:Polysaccharides / Q000494:pharmacology*; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D015044:Zona Pellucida / Q000187:drug effects / Q000502:physiology*
D003911:Dextrans; D011134:Polysaccharides; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D016264:Dextran Sulfate; C007789:fucoidan