Polyamines in rat hepatocyte cultures.
In rat hepatocytes cultured for 120 h polyamine content was markedly modified. Putrescine concentration reached a maximum at 48 h, spermidine increased for 48 h and then remained constant, spermine after a decrease returned to its initial values. Total polyamine amount was increased by 75%. Both ornithine decarboxylase and the retroconversion pathway were responsible for these modifications. The possible correlation between polyamine metabolism and retrodifferentiation process was investigated by studying them in conditions which are known to preserve differentiated functions.
Dipartimento di Medicina e Oncologia Sperimentale, Università di Torino, Italy.
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S
Colombatto
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MA
Grillo
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D000518:Eflornithine / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008935:Mitoguazone / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D051381:Rats; D013096:Spermine / Q000502:physiology; D014212:Tretinoin / Q000494:pharmacology; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D011073:Polyamines; D013096:Spermine; D014212:Tretinoin; D005723:gamma-Glutamyltransferase; D009955:Ornithine Decarboxylase; D008935:Mitoguazone; D004121:Dimethyl Sulfoxide; D000518:Eflornithine