Combined use of RFLP and PCR-ASO typing for HLA-DR-Dw and DQw typing.
Due to some limitations of restriction fragment length polymorphism (RFLP) analysis in HLA-DR-DQ typing, we present a combined use of RFLP and polymerase chain reaction (PCR)-allele-specific oligonucleotide (ASO) typing. This scheme consists in selectively amplifying the few RFLP ill-defined genes (DR1/DR'Br' and DR4-Dw subsets) using PCR with allele specific primers to avoid cross-hybridization.
Bignon
J D
JD
Laboratoire d'Immunologie Leuco-plaquettaire et d'Histocompatibilité 'France-Transplant', Nantes, France.
Bidwell
J L
JL
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D015342:DNA Probes; D006683:HLA-DQ Antigens / Q000145:classification / Q000235:genetics*; D006684:HLA-DR Antigens / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D009838:Oligodeoxyribonucleotides