Lytic, agglutinating, and opsonizing effect of alpha 2-macroglobulin on sheep red blood cells.
Mouse alpha 2-macroglobulin (alpha 2M) induced agglutination and lysis of sheep red blood cells depending on temperature and time of incubation in vitro. When these erythrocytes (E) were treated with a subagglutinanting dose of alpha 2M, they were adhered to and phagocytosed by thioglycollate-elicited and BCG-activated mouse peritoneal macrophages. Phagocytosis was not observed when resident peritoneal macrophages were tested. alpha 2M also was able to dissociate sheep red blood cells previously aggregated by IgM anti-E.
Departamento de Immunologia, Universidade de São Paulo, Brazil.
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Isaac
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Mariano
D000818:Animals; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D006384:Hemagglutination / Q000187:drug effects; D007075:Immunoglobulin M / Q000276:immunology*; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology; D051379:Mice; D010587:Phagocytosis; D012756:Sheep / Q000097:blood; D000511:alpha-Macroglobulins / Q000494:pharmacology*
D007075:Immunoglobulin M; D000511:alpha-Macroglobulins