Identification and characterization of monoclonal antibodies reactive with bovine, caprine and ovine T-lymphocyte determinants by flow microfluorimetry.
Comparative flow microfluorimetric (FMF) analysis was used to identify and characterize 27 monoclonal antibodies (MoAbs) reactive with bovine T-lymphocytes. Determinants present on all circulating T-lymphocytes were recognized by 11 MoAbs, 8 of which blocked E rosette formation. Determinants present on only the BoCD4+ T-lymphocyte subset were detected by 9 MoAbs, while determinants restricted to the BoCD8+ T-lymphocyte subset were recognized by 7 MoAbs. Competitive labeling experiments demonstrated that determinants recognized by subset-specific MoAbs were present on BoCD4 or BoCD8 molecules. Comparative studies revealed that some determinants, both pan-T specific and subset-specific, were conserved on homologous (orthologous) molecules expressed on leukocytes from other species of ruminants. Polymorphism was evident with several determinants.
Larsen
R A
RA
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington St. University, Pullman 99164-7040.
Monaghan
M L
ML
Park
Y H
YH
Hamilton
M J
MJ
Ellis
J A
JA
Davis
W C
WC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D005434:Flow Cytometry; D005470:Fluorometry; D006041:Goats; D007132:Immunoglobulin Isotypes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011110:Polymorphism, Genetic; D012397:Rosette Formation; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000939:Epitopes; D007132:Immunoglobulin Isotypes