Cross-reactive protection against influenza A virus infections by an NS1-specific CTL clone.
An influenza A subtype cross-reactive CTL clone (A-11) was established following stimulation of A/PR/8 virus-immune spleen cells of Balb/C (H-2d) mice. This T cell clone lysed target cells infected with influenza viruses of the H1, H2, or H3 subtypes, and recognizes a conserved epitope on the NS1 protein. The clone is restricted by the H-2Ld allele. Adoptive transfer of A-11 significantly reduced virus titers in the lungs of mice infected with influenza A viruses of the H1, H2, or H3 subtypes. These results suggest that the conserved epitope on NS1 which is recognized by A-11 may be a useful component to consider for inclusion in experimental cross-reactive influenza vaccines.
Kuwano
K
K
Department of Medicine, University of Massachusetts Medical School, Worcester 01655.
Tamura
M
M
Ennis
F A
FA
D000483:Alleles; D000818:Animals; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D002999:Clone Cells; D003429:Cross Reactions; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000382:microbiology; D008285:Major Histocompatibility Complex / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000235:genetics / Q000276:immunology*; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology*; D017361:Viral Nonstructural Proteins
D000939:Epitopes; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins