Mutagenicity of airborne particles from four cities in Taiwan.
The mutagenicity of airborne particles from 8 urban and suburban locations in each of four cities, Taipei, Hsinchu, Taichung, and Kaohsiung, in Taiwan area were investigated with S. typhimurium strain TA98 by Ames Salmonella/microsomal test. The average mutagenic activity of airborne particulate samples from Taipei and Kaohsiung was higher than that from Hsinchu and Taichung with or without metabolic activation. The major direct-acting mutagenic compounds of airborne particulate samples from Taipei and Kaohsiung was similar to that of standard dinitropyrenes mixture (DNPs) in the retention time of HPLC. Moreover, the contents of DNPs of airborne particulate samples from Taipei and automobile exhaust partially purified through Sephadex LH-20 gel filtration and semipreparative HPLC were determined by HPLC. DNPs was major direct-acting mutagens of the urban air samples from Taipei and their major pollutants might be from automobile exhaust. However, the major mutagenic compounds of airborne particulate samples from Hsinchu and Taichung did not correspond to any of the standard compounds tested. The content of benzo[a]pyrene (B[a]P) of airborne particulate samples was also determined by HPLC. The concentration of B[a] P was 0.05-0.62 ng/m3 air sample. The B[a] P contents of airborne particulate samples from four cities in Taiwan did not show good correlation with their mutagenic activity. Thus, we concluded that B[a] P was not a major indirect-acting mutagenic compound in the tested air samples.
Department of Medicine, Chung Shan Medical & Dental College, Taichung, Taiwan, R.O.C.
M C
MC
Chou
H
H
Lee
D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity*; D001564:Benzo(a)pyrene / Q000032:analysis / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis / Q000633:toxicity*; D011721:Pyrenes / Q000032:analysis / Q000633:toxicity; D012486:Salmonella typhimurium; D013624:Taiwan; D001335:Vehicle Emissions / Q000032:analysis / Q000633:toxicity
D000393:Air Pollutants; D009153:Mutagens; D011721:Pyrenes; D001335:Vehicle Emissions; D001564:Benzo(a)pyrene