Evaluation of UW solution in a rat kidney preservation model. I. Effect of hydroxyethyl starch and electrolyte composition.
Preservation of rat kidneys by simple ice-storage has been demonstrated after 48 hr using the University of Wisconsin (UW) solution; hypothermic preservation of the rat kidney was dramatically improved. A modified UW solution without hydroxyethyl starch (HES) also gave uniform survival after 48 hr of storage, and even better function and morphology. Substitution of HES from another source also improved renal function when compared with UW (Dupont) and it is suggested that prolonged storage of UW solution prior to use may reduce its effectiveness. Reversing the Na:K ratio of the solution still allowed successful preservation, but significantly worsened morphology of the surviving kidney.
Department of Surgery, Monash University, Prince Henry's Hospital, Melbourne, Australia.
M
M
Biguzas
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Jablonski
A C
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Thomas
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K
Walls
B O
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Howden
D F
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Scott
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VC
Marshall
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003404:Creatinine / Q000097:blood; D004573:Electrolytes; D005978:Glutathione; D006895:Hydroxyethyl Starch Derivatives; D007328:Insulin; D016030:Kidney Transplantation / Q000379:methods*; D009395:Nephritis, Interstitial / Q000473:pathology; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions; D013213:Starch / Q000031:analogs & derivatives; D014508:Urea / Q000097:blood
D004573:Electrolytes; D006895:Hydroxyethyl Starch Derivatives; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D014508:Urea; D013213:Starch; D003404:Creatinine; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose