Effects of neonatal bilateral eye enucleation on postnatal development of the monoamines in posterior thalamus of the rat.
Levels of dopamine (DA), noradrenaline (NA) and 5-hydroxytryptamine (serotonin, 5-HT) and their metabolites, and the activities of tyrosine hydroxylase (TH), tryptophan hydroxylase (TPH) and monoamine oxidase A and B (MAO-A and MAO-B) have been determined in the rat posterior thalamus after enucleation during postnatal development. DA and 5-HT turnover rate have been measured as 3,4-dihydroxyphenylalanine (DOPA) and 5-hydroxytryptophan (5-HTP) accumulation rates after central decarboxylase inhibition by 3-hydroxybenzylhydrazine (NSD-1015). The major changes were an increase in noradrenergic and serotoninergic metabolism in enucleated animals compared with control animals. A decrease of the MAO-A to MAO-B ratio during postnatal development was found.
Departamento de Bioquímica, Bromatología y Toxicología, Universidad de Sevilla, Spain.
M L
ML
Vizuete
M
M
Santiago
A J
AJ
Herrera
J L
JL
Venero
A
A
Machado
J
J
Cano
D006916:5-Hydroxytryptophan / Q000378:metabolism; D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism; D015306:Biogenic Monoamines / Q000378:metabolism*; D004295:Dihydroxyphenylalanine / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D015353:Eye Enucleation; D006834:Hydrazines / Q000494:pharmacology; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism; D013788:Thalamus / Q000254:growth & development* / Q000378:metabolism; D014365:Tryptophan Hydroxylase / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D015306:Biogenic Monoamines; D006834:Hydrazines; D012701:Serotonin; D004295:Dihydroxyphenylalanine; C004297:3-hydroxybenzylhydrazine; D006916:5-Hydroxytryptophan; D014446:Tyrosine 3-Monooxygenase; D014365:Tryptophan Hydroxylase; D008995:Monoamine Oxidase; D001142:Aromatic-L-Amino-Acid Decarboxylases; D004298:Dopamine; D009638:Norepinephrine