Excitatory synaptic involvement in epileptiform bursting in the immature rat neocortex.
1. Neocortical brain slices were prepared from animals 8-15 days of age and maintained in vitro. Intracellular recordings were obtained from neurons in cortical layers 2-3. The role of synaptic activity and excitatory amino acid receptors in generation of picrotoxin-induced ictal-like epileptiform activity in the immature neocortex was investigated. D-2-amino-5-phosphonovaleric acid (D-APV) and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) were used as selective antagonists of N-methyl-D-aspartate (NMDA) and non-NMDA receptors, respectively. 2. Ictal-like epileptiform discharges were induced by bath application of the GABAA-receptor antagonist picrotoxin. Paroxysmal discharges, 7-25 s in duration, occurred spontaneously or could be evoked by electrical stimulation. These events consisted of an initial paroxysmal depolarizing shift (PDS) followed by a long-duration depolarization (LLD) with superimposed late PDSs. 3. The amplitudes of the initial PDS, LLD, and late PDSs were linearly dependent on membrane potential, increasing with hyperpolarization and diminishing on depolarization. All responses reversed polarity near 0 mV. Under voltage-clamp conditions, both transient and sustained currents were observed, coincident with PDSs and the LLD, respectively. The duration of the ictal-like events was similar under current- and voltage-clamp conditions, suggesting activation of intrinsic membrane currents did not significantly prolong epileptiform discharges. 4. Bath application of D-APV (20 microM) decreased the amplitude and duration of both the initial PDS and LLD without affecting the time-to-onset of epileptiform activity.(ABSTRACT TRUNCATED AT 250 WORDS)
Lee
W L
WL
Neurobiology Research Center, University of Alabama, Birmingham 35294.
Hablitz
J J
JJ
D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology*; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D000375:Aging; D000818:Animals; D002540:Cerebral Cortex / Q000254:growth & development / Q000502:physiology*; D004827:Epilepsy / Q000503:physiopathology*; D005071:Evoked Potentials / Q000187:drug effects; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010852:Picrotoxin / Q000494:pharmacology; D011810:Quinoxalines / Q000494:pharmacology*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
D015763:2-Amino-5-phosphonovalerate; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D010852:Picrotoxin; D011810:Quinoxalines; D016194:Receptors, N-Methyl-D-Aspartate