A mitochondrial protein fraction catalyzing transport of the K+ analog T1+.
A protein fraction has been obtained from detergent-solubilized mitochondrial membranes by its affinity for quinine, an inhibitor of K+ transport. A peptide derived from the predominant 53 kDa protein in this fraction is found to be identical in sequence to a portion of aldehyde dehydrogenase. Antigenically unrelated bands at 97, 77, 57, and 31 kDa are also seen on polyacrylamide gels. Observations utilizing a fluorescent probe entrapped in the lumen of membrane vesicles indicate that the reconstituted protein fraction imparts permeability to the K+ analog Tl+. These and other findings suggest that the affinity purified fraction includes a cation transport catalyst.
Biology Department, Rensselaer Polytechnic Institute, Troy, NY 12180-3590.
J J
JJ
Diwan
R
R
Paliwal
E
E
Kaftan
R
R
Bawa
D000444:Aldehyde Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active; D000939:Epitopes / Q000032:analysis; D005456:Fluorescent Dyes; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D008970:Molecular Weight; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D013367:Submitochondrial Particles / Q000378:metabolism*; D013793:Thallium / Q000378:metabolism*; D014634:Valinomycin / Q000494:pharmacology
D000939:Epitopes; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D014634:Valinomycin; D013793:Thallium; D000444:Aldehyde Dehydrogenase; D011188:Potassium