Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells.
Endothelial leukocyte adhesion molecule-1 (ELAM-1) is an endothelial cell adhesion molecule that allows myeloid cells to attach to the walls of blood vessels adjacent to sites of inflammation. ELAM-1 recognizes the sialyl-Lewis X (sialyl-Lex) determinant, NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc-, a granulocyte carbohydrate also found on the surface of some tumor cell lines. Binding of myeloid cells to soluble ELAM-1 is inhibited by a monoclonal antibody recognizing sialyl-Lex or by proteins bearing sialyl-Lex, some of which may participate in humoral regulation of myeloid cell adhesion. Stimulated granulocytes also release an inhibitor of ELAM-1 binding that can be selectively adsorbed by monoclonal antibody to sialyl-Lex.
Walz
G
G
Department of Genetics, Harvard Medical School, Boston, MA 02114.
Aruffo
A
A
Kolanus
W
W
Bevilacqua
M
M
Seed
B
B
D000653:Amniotic Fluid / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002240:Carbohydrate Sequence; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000378:metabolism; D005643:Fucose / Q000378:metabolism; D005647:Fucosyltransferases / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism*; D007163:Immunosorbent Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D016209:Interleukin-8 / Q000494:pharmacology; D016256:Lewis X Antigen / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry; D009961:Orosomucoid / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007375:Interleukin-1; D016209:Interleukin-8; D016256:Lewis X Antigen; D009844:Oligosaccharides; D009961:Orosomucoid; D000080506:Sialyl Lewis X Antigen; D005643:Fucose; D005647:Fucosyltransferases; D009439:Neuraminidase