Characterization of a rat monoclonal antibody specific for a determinant encoded by the V beta 7 gene segment. Depletion of V beta 7+ T cells in mice with Mls-1a haplotype.
We have generated a rat mAb, TR310, which recognizes a determinant encoded by the murine V beta 7 gene segment of the TCR. TR310 immunoprecipitates TCR from cell lysates, co-modulates with CD3, and can be used for immunofluorescence staining of T cells. By using this antibody, we found that the average percentage of V beta 7+ peripheral T cells in Mls-1b mice was 3.8%, but only 0.8% in Mls-1a mice. A similar difference was also observed in the mature TCRhi thymocyte subsets, suggesting that V beta 7+ T cells are deleted during intrathymic maturation in Mls-1a mice. TR310 should prove to be a valuable reagent in further studies of the TCR repertoire and the analysis of factors which alter it.
Okada
C Y
CY
Department of Pathology, Stanford University School of Medicine, CA 94305-5020.
Holzmann
B
B
Guidos
C
C
Palmer
E
E
Weissman
I L
IL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D017252:CD3 Complex; D000939:Epitopes; D005434:Flow Cytometry; D007108:Immune Tolerance; D051379:Mice; D008815:Mice, Inbred Strains; D008912:Minor Histocompatibility Loci; D016747:Minor Lymphocyte Stimulatory Antigens; D011233:Precipitin Tests; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D001324:Autoantigens; D017252:CD3 Complex; D000939:Epitopes; D016747:Minor Lymphocyte Stimulatory Antigens; D011948:Receptors, Antigen, T-Cell