Isolation of a family of Drosophila POU domain genes expressed in early development.
The POU domain is a approximately 140 amino acid domain shared by several mammalian transcriptional activators. We have isolated genomic and cDNA clones from three Drosophila POU domain genes, designated pdm-1, pdm-2, and Cf1a. All three genes encode a conserved POU-specific domain and a POU homeo domain but are otherwise divergent. By Northern analysis, all three of the genes are expressed in early embryos; the pdm-1 and Cf1a genes are also expressed at lower levels throughout development. The spatial expression patterns of the pdm-2 and Cf1a genes show that they probably play multiple roles during development: an early role in specific ectodermal cells, and a subsequent role in the embryonic nervous system.
Department of Biological Sciences, University of California, Santa Barbara 93106.
A N
AN
Billin
K A
KA
Cockerill
S J
SJ
Poole
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D015870:Gene Expression; D005796:Genes / Q000235:genetics*; D005801:Genes, Homeobox / Q000235:genetics*; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics
D004247:DNA