Ethanol-induced smooth and skeletal muscle myopathy: use of animal studies.
This article reviews the effects of ethanol on skeletal and smooth muscle. A brief summary of its clinical effects is provided, with a rationale for the use of suitable animal models to study ethanol-induced myotoxicity. Practical details are given for the animal feeding techniques to examine the chronic effects of ethanol toxicity. Information on acute ethanol dosage experiments are also provided. Our results have indicated that ethanol causes net loss of both skeletal and smooth muscle protein and an effect on protein synthesis and/or degradation was implicated. The theoretical and practical basis of measuring protein synthesis in intact laboratory animals is reviewed. However, there are no reliable methods for measuring rates of protein breakdown in vivo. The combined results of our studies indicated that disturbances in protein synthesis were causal mechanisms for ethanol-induced myo-dysfunction. Acute ethanol exposure was largely characterised by reductions in fractional rates of skeletal and smooth muscle contractile protein synthesis. The dominant characteristics of chronic treatments were loss of skeletal and smooth muscle proteins and RNA. Further laboratory animal studies will eventually elucidate the molecular mechanisms of these changes and provide valuable information on the regulation of protein mass.
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, London, U.K.
V R
VR
Preedy
J S
JS
Marway
A J
AJ
Macpherson
T J
TJ
Peters
D000205:Actomyosin / Q000096:biosynthesis; D000428:Alcohol Drinking / Q000503:physiopathology; D000435:Alcoholic Intoxication / Q000503:physiopathology; D000437:Alcoholism / Q000503:physiopathology*; D000818:Animals; D001823:Body Composition / Q000502:physiology; D003285:Contractile Proteins / Q000096:biosynthesis; D004195:Disease Models, Animal; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis*; D009130:Muscle, Smooth / Q000503:physiopathology*; D009132:Muscles / Q000503:physiopathology*; D009133:Muscular Atrophy / Q000503:physiopathology*; D012313:RNA / Q000096:biosynthesis; D051381:Rats
D003285:Contractile Proteins; D009124:Muscle Proteins; D012313:RNA; D000205:Actomyosin