Applications of polymerase chain reaction methods in genome mapping.
Important new polymerase chain reaction-based techniques have been developed to assist in genome analysis. Applications range from genetic and physical mapping of DNA to sequence analysis. The polymerase chain reaction has played a significant role in increasing the feasibility of many aspects of genome analysis and positional cloning.
Nelson
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Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular / Q000379:methods; D004252:DNA Mutational Analysis; D005819:Genetic Markers; D005822:Genetic Vectors; D016678:Genome; D015698:Genomic Library; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae; D016324:Sequence Tagged Sites
D005819:Genetic Markers