The genomic diversity and stability of field strains of suid herpesvirus 1 (Aujeszky's disease virus).
The genomic diversity among isolates of suid herpesvirus 1 (SHV-1) collected in the same herd and among clones from the same isolate was studied by restriction fragment pattern (RFP) analysis using BamHI. Tentatively defining a field strain as a transmissible entity, it was concluded that strains of SHV-1 commonly comprise distinguishable genomic variants. Contrary to the hypothesis of genomic lability, it is suggested that the pool of variants is sufficiently stable to specifically characterize a strain. The impact of the results on epizootiological methods based on identification of field isolates is discussed in connection with Aujeszky's disease in Denmark.
Christensen
L S
LS
State Veterinary Institute for Virus Research, Kalvehave, Denmark.
Sørensen
K J
KJ
D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003718:Denmark / Q000453:epidemiology; D015245:Deoxyribonuclease BamHI; D004196:Disease Outbreaks / Q000662:veterinary*; D014644:Genetic Variation; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D011557:Pseudorabies / Q000453:epidemiology / Q000382:microbiology*; D015183:Restriction Mapping; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*
D015245:Deoxyribonuclease BamHI