Histamine release from human peripheral blood leukocytes analyzed by histamine radioimmunoassay.
Histamine release from human peripheral blood leukocytes, after in vitro challenge with allergen extracts, is usually measured by fluorometry. In the present study we compared the results of the automated fluorometric histamine assay (Siraganian) with those of the Immunotech histamine radioimmunoassay. Histamine release dose-response curves obtained after histamine measurement by both methods were superimposable.
Laboratory of Allergology (Department of Clinical Chemistry), Erasmus University Hospital-Dijkzigt, Rotterdam, The Netherlands.
A W
AW
van Toorenenbergen
A M
AM
Vermeulen
D006632:Histamine / Q000032:analysis; D006636:Histamine Release; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000378:metabolism*; D011863:Radioimmunoassay; D013050:Spectrometry, Fluorescence
D006632:Histamine