Enhancement of an anti-tumor effect of interferon by dipyridamole in established human malignant melanoma cell lines.
Enhancement of the anti-proliferative effect of human interferon (HuIFN) preparations (alpha, beta and gamma) by dipyridamole was detected in a human malignant melanoma cell line, MM-ICB, which we originally established. Cell growth was inhibited by HuIFN alone, but a marked increase in inhibition was noted in vitro and in vivo when dipyrydamole was added. Cellular DNA synthesis, as determined by 3H-deoxythymidine incorporation into the acid-insoluble cellular fraction, was more inhibited by combined treatment than by any of the agents used alone. Two other melanoma cell lines that we established, MM-2CB and MM-3CB, also exhibited sensitivity to combined treatment both in vitro and in vivo. Furthermore, the HMV-I and SEKI melanoma cell lines were susceptible to the combination. Even non-cytotoxic concentrations of dipyridamole could enhance the effect of HuIFN on MM-ICB, MM-2CB, and SEKI cells.
Department of Dermatology, School of Medicine, Chiba University, Japan.
T
T
Kojima
N
N
Suzuki
I
I
Sugano
I
I
Hayata
D000368:Aged; D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D004176:Dipyridamole / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology*; D008297:Male; D008545:Melanoma / Q000188:drug therapy*; D051379:Mice; D008815:Mice, Inbred Strains; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D007370:Interferon Type I; D004176:Dipyridamole; D007372:Interferons