High-performance liquid chromatographic determination of proteins by post-column fluorescence derivatization with thiamine reagent.
A post-column derivatization method for the high-performance liquid chromatography of peptides and proteins giving a fluorescence intensity proportional to the number of peptide bonds is described. Peptide bonds were chlorinated with hypochlorite and the N-chlorite formed was allowed to react with thiamine to give fluorescent thiochrome. This method was applied the determination of membrane-forming proteins of microorganisms.
Yokoyama
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Central Research Laboratory, SS Pharmaceutical Co. Ltd., Chiba, Japan.
Kinoshita
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T
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003574:Cytochrome c Group / Q000032:analysis; D005453:Fluorescence; D009113:Muramidase / Q000032:analysis; D009211:Myoglobin / Q000032:analysis; D010047:Ovalbumin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine; D013831:Thiamine; D013954:Thyroglobulin / Q000032:analysis; D005719:gamma-Globulins / Q000032:analysis
D003574:Cytochrome c Group; D009211:Myoglobin; D011506:Proteins; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D013954:Thyroglobulin; D009113:Muramidase; D013831:Thiamine