A morphological study on glutamate-induced swelling of cultured astrocytes: involvement of calcium and chloride ion mechanisms.
Morphological changes in cultured astrocytes exposed to L-glutamate (Glu) were examined light and electron microscopically. The treatment with 0.1 mM Glu for 60 min caused marked swelling of the cells, which was characterized by reduction in staining of cytoplasm with Toluidine blue, disappearance of the cytoplasmic granular ground substances, swollen mitochondrion and nucleus, and dispersed chromatin. The above changes were prevented by the removal of Na+, Ca2+ or Cl- from the incubation medium for Glu treatment. However, the Glu treatment in a Cl(-)-free medium caused conspicuous aggregation of 10 nm filaments.
Koyama
Y
Y
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Sugimoto
T
T
Shigenaga
Y
Y
Baba
A
A
Iwata
H
H
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000648:ultrastructure; D002118:Calcium / Q000502:physiology*; D002467:Cell Nucleus / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects; D002712:Chlorides / Q000502:physiology*; D002843:Chromatin / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000187:drug effects; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D066298:In Vitro Techniques; D008933:Mitochondrial Swelling / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014048:Tolonium Chloride
D002712:Chlorides; D002843:Chromatin; D005971:Glutamates; D014048:Tolonium Chloride; D018698:Glutamic Acid; D002118:Calcium