Analysis of variation in batches of armadillo-derived Mycobacterium leprae by immunoblotting.
Several batches of cell-free extracts of armadillo-derived Mycobacterium leprae were analyzed by SDS-PAGE and by immunoblotting with monoclonal antibodies. The presence or absence of protease inhibitors had a profound effect on the protein antigens, particularly the 65-kDa antigen. In the absence of protease inhibitors, there were both quantitative and qualitative differences between the different batches of M. leprae extracts.
Laboratory for Leprosy and Mycobacterial Research, National Institute for Medical Research, Mill Hill, London, U.K.
M A
MA
Ibrahim
F I
FI
Lamb
M J
MJ
Colston
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D001135:Armadillos; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D015151:Immunoblotting; D008970:Molecular Weight; D009166:Mycobacterium leprae / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes