Structural differences among guinea pig Fc gamma 1/gamma 2 receptors on macrophages, polymorphonuclear cells, and lymphocytes.
Using the cDNA, D-3, coding for Fc gamma 1/gamma 2 receptor of guinea pig macrophages that binds IgG1 and IgG2 (Fc gamma 1/gamma 2R), we examined the cell distribution of this receptor by RNA blot analysis. The Fc gamma 1/gamma 2R mRNA was expressed in polymorphonuclear cells and B cells as well as in macrophages, but not at the detectable level in T cells. The cDNA amplified from RNA of polymorphonuclear cells in the polymerase chain reaction was the same as D-3. The cDNA of B cells was found to have about 140 bp cDNA segment inserted to the cytoplasmic tail of D-3. We found that the cDNA amplified from T cell RNA differed in signal peptide and extracellular domain sequence from cDNAs of other cell types. This cDNA does not seem to be amplified from the mRNAs of contaminating other cell types.
Department of Biochemistry, Kumamoto University Medical School, Japan.
A
A
Sakata
M
M
Tominaga
T
T
Ohmura
K
K
Onoue
D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics*; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D006168:Guinea Pigs; D008264:Macrophages / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011961:Receptors, Fc / Q000235:genetics*; D017452:Receptors, IgG; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000276:immunology*; D014158:Transcription, Genetic
D000943:Antigens, Differentiation; D012333:RNA, Messenger; D011961:Receptors, Fc; D017452:Receptors, IgG; D012313:RNA; D004247:DNA