Role of lymphocyte activation by substance P in rheumatoid arthritis.
Peripheral mononuclear cells taken from rheumatoid arthritis (RA) patients, which had previously been treated in vitro with the neuropeptide Substance P (SP) followed by stimulation with the mitogen PHA, showed a significantly higher percent positivity of lymphocytes with the double-markers CD4+ CD2+, CD8+ CD25+, CD4+, HLA-DR+ and CD8+ HLA-DR+ than those of the cells untreated by SP. No effect of SP was detected in normal controls, except that the cells with the double-marker CD4+ CD25+ were slightly increased. Furthermore, in RA patients the percentage of CD4+ CD45R- cells was increased, while that of CD4+ CD45R+ cells was decreased, by pre-treatment with SP and stimulation with PHA. Monocytes of patients with rheumatoid arthritis after the treatment with SP released a significantly higher amount of oxygen-intermediate than those of normal controls with or without SP. From these results, it could be considered that SP plays an important role in RA inflammation.
Yokoyama
M M
MM
Department of Immunology, Kurume University School of Medicine, Fukuoka, Japan.
Fujimoto
K
K
D015703:Antigens, CD; D000943:Antigens, Differentiation; D001172:Arthritis, Rheumatoid / Q000209:etiology / Q000276:immunology* / Q000378:metabolism; D006684:HLA-DR Antigens; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D013373:Substance P / Q000494:pharmacology* / Q000502:physiology
D015703:Antigens, CD; D000943:Antigens, Differentiation; D006684:HLA-DR Antigens; D010835:Phytohemagglutinins; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D013373:Substance P; D010100:Oxygen