An improved method for the preparation of rat cerebellar glomeruli.
Cerebellar glomeruli consist of large portions of the mossy fiber giant terminal, granule cell dendrites and Golgi neuron terminals. By modifying previously reported procedures we have developed a new method for bulk preparation of this polysynaptic complex from rat cerebellum. We obtained well preserved isolated glomeruli of satisfactory purity and homogeneity as indicated by electron microscopy and by determination of appropriate biochemical markers. The method is fast and simple, and it provides a glomerular fraction suitable for investigation of neurotransmitter receptors.
Viennot
F
F
Centre de Neurochimie du CNRS and INSERM U.44, Strasbourg, France.
Artault
J C
JC
Tholey
G
G
De Barry
J
J
Gombos
G
G
D000818:Animals; D001253:Astrocytes / Q000737:chemistry; D015415:Biomarkers; D002531:Cerebellum / Q000737:chemistry / Q000648:ultrastructure*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D006652:Histological Techniques; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000737:chemistry / Q000648:ultrastructure*; D009412:Nerve Fibers / Q000737:chemistry / Q000648:ultrastructure*; D009474:Neurons / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000737:chemistry / Q000648:ultrastructure
D015415:Biomarkers; D005971:Glutamates; D018698:Glutamic Acid