[Distribution of F-actin (DACM-HMM staining) in the epidermis in normal subjects and in cases of psoriasis vulgaris].
The epithelium of normal subjects (NE) and that in cases of psoriasis vulgaris, or PV, (PVE) were studied using DACM-HMM staining. In the living cell layer of NE, strong fluorescence was observed in the cell margins, and these bands of fluorescence were narrower in the granular layer than in the basal and squamous layers. In PV, the bands were broad in the lower and middle strata of the squamous layer, and attenuated in the upper strata. Accompanying keratinization, the distribution of F-actin in the keratinocytes was thought to vary. The variations in fluorescence in the lower and middle squamous layer strata of PVE may be attributed to increase of F-actin in the cell margins.
Tanaka
K
K
Department of Dermatology, Nihon University School of Medicine.
D000199:Actins / Q000378:metabolism*; D004817:Epidermis / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D008301:Maleimides; D015879:Myosin Subfragments; D011565:Psoriasis / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D013194:Staining and Labeling
D000199:Actins; D007202:Indicators and Reagents; D008301:Maleimides; D015879:Myosin Subfragments; C019347:N-(7-dimethylamino-4-methylcoumarinyl)maleimide