A murine monoclonal antibody exhibiting high species specificity for Histoplasma capsulatum var. capsulatum.
A monoclonal antibody (mAb) exhibiting a high degree of species specificity for the yeast phase of the dimorphic fungus Histoplasma capsulatum was produced by a modification of the standard mAb production protocol. The technique for generating mAbs involved the use of the immunosuppressive drug cyclophosphamide to diminish the response in mice to immunodominant cross-reactive epitopes. This mAb exhibited clear specificity and did not react by ELISA with the closely related genera Blastomyces, Paracoccidioides and Sporothrix. In Western blots it recognized a linear determinant on a 70-75 kDa molecule in H. capsulatum antigen, with an extremely faint reactivity to antigens of identical molecular mass derived from Sporothrix and Paracoccidioides, and no reactivity against Blastomyces antigen.
Hamilton
A J
AJ
Dermatology Unit, Clinical Sciences Laboratories, Guy's Hospital, London, UK.
Bartholomew
M A
MA
Fenelon
L E
LE
Figueroa
J
J
Hay
R J
RJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006658:Histoplasma / Q000276:immunology*; D006660:Histoplasmosis / Q000175:diagnosis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D000939:Epitopes