Molecular cloning of human lysyl oxidase and assignment of the gene to chromosome 5q23.3-31.2.
Lysyl oxidase (EC 1.4.3.13) initiates the crosslinking of collagens and elastin by catalyzing oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues. We report here on the isolation and characterization of cDNA clones for the enzyme from human placenta and rat aorta lambda gt11 cDNA libraries. A cDNA clone for human lysyl oxidase covers all the coding sequences, 230 nucleotides of the 5' and 299 nucleotides, of the 3' untranslated sequences, including a poly(A) tail of 23 nucleotides. This cDNA encodes a polypeptide of 417 amino acid residues, including a signal peptide of 21 amino acids. Sequencing of two rat lysyl oxidase cDNA clones indicated six differences between the present and the previously published sequence for the rat enzyme [Trackman et al. (1990) Biochemistry 29: 4863-4870], resulting in frameshifts in the translated sequence. The human lysyl oxidase sequence was found to be 78% identical to the revised rat sequence at the nucleotide level and 84% identical at the amino acid level, with the degree of identity unevenly distributed between various regions of the coded polypeptide. Northern blot analysis of human skin fibroblasts RNA indicated that the human lysyl oxidase cDNA hybridizes to at least four mRNA species; their sizes are about 5.5, 4.3, 2.4, and 2.0 kb. Analysis of a panel of 25 human x hamster cell hybrids by Southern blotting mapped the human lysyl oxidase gene to chromosome 5, and in situ hybridization mapped it to 5q23.3-31.2.(ABSTRACT TRUNCATED AT 250 WORDS)
Collagen Research Unit, University of Oulu, Finland.
E R
ER
Hämäläinen
T A
TA
Jones
D
D
Sheer
K
K
Taskinen
T
T
Pihlajaniemi
K I
KI
Kivirikko
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002478:Cells, Cultured; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D006224:Cricetinae; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D008249:Protein-Lysine 6-Oxidase / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D016415:Sequence Alignment
D008249:Protein-Lysine 6-Oxidase