Early activation of heat shock genes in H2O2-treated Drosophila cells.
Drosophila cells of a diploid clone derived from line Kc were treated with 1 mM H2O2 for 1 to 20 minutes. Dot blot and Northern blot analysis of RNAs extracted from control and treated cells showed that the transcriptional activation of the 6 heat-shock genes tested was early, and maximal within 5 minutes of H2O2 treatment. Analysis of the kinetics of induction of the heat-shock proteins (hsps) after an exposure to H2O2 of 2 or 5 minutes, followed by removal, suggests that this brief treatment was sufficient to trigger the synthesis of all the hsps, which was maximal 1.5 to 3h after this short H2O2 treatment.
Unité Associée au CNRS 1135, Université Pierre et Marie Curie, Paris, France.
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Courgeon
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Becker
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Maingourd
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Maisonhaute
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Best-Belpomme
D000818:Animals; D002460:Cell Line; D015342:DNA Probes; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis; D014158:Transcription, Genetic / Q000187:drug effects
D015342:DNA Probes; D006360:Heat-Shock Proteins; D012313:RNA; D006861:Hydrogen Peroxide