In vitro differentiation and proliferation of purified human thymic and bone marrow CD7+CD2- T-cell precursors.
It is well established that CD7, gp40 antigen is one of the first antigens detected on the surfaces of cells of the human T-cell lineage. Using complement-dependent cytotoxicity and immunoadherence to anti-CD7-coated surfaces, we were able to purify CD7+2-3-4-8-TcR- cells with greater than 90% purity from both human thymus and bone marrow. Limiting dilution analysis showed that these cells displayed high ability to generate mature T-cell clones when they were cultured in the appropriate conditions. These precursors needed phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM) as a differentiation signal before being able to respond to PHA and recombinant interleukin 2 (rIL2). CD7+CD2- precursors differed from more mature CD7+CD2+ thymocytes because they were not sensitive to PHA, IL2, or CD2 triggering. Bone marrow-derived clones were mostly CD4+, whereas thymic cells generated more CD8+ than CD4+ clones. Together, this study indicates that the CD7+CD2- precursor is one of the earliest prothymocytes able to differentiate and proliferate in vitro.
Mossalayi
M D
MD
CNRS URA186, Pitié-Salpêtrière Hospital, Paris, France.
Dalloul
A H
AH
Bertho
J M
JM
Lecron
J C
JC
Goube de Laforest
P G
PG
Debré
P
P
D015703:Antigens, CD / Q000032:analysis; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D001854:Bone Marrow Cells; D018801:CD2 Antigens; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002675:Child, Preschool; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology; D010641:Phenotype; D011971:Receptors, Immunologic / Q000032:analysis*; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology*
D015703:Antigens, CD; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D008222:Lymphokines; D011971:Receptors, Immunologic