In situ separation of nucleolar components by hypotonic treatment of cells.
The main features of the reaction of nucleolar components to a simple hypotonic shock are described. In place of nucleoli, only fibrillar complexes remain. Such preservation provides another morphological proof of the hypothesis of fibrillar complexes as morphological-functional units in active nucleoli. Moreover, this method can become a good tool for further study of nucleolus-organizing regions.
Institute of Experimental Medicine, Czechoslovak Academy of Sciences, Prague.
P
P
Hozák
O V
OV
Zatsepina
Z
Z
Likovský
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002466:Cell Nucleolus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D007038:Hypotonic Solutions / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D009697:Nucleolus Organizer Region / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010710:Phosphates / Q000494:pharmacology*; D051381:Rats; D012834:Silver; D013194:Staining and Labeling
D007038:Hypotonic Solutions; D010710:Phosphates; D012834:Silver