HMG1-related DNA-binding protein isolated with V-(D)-J recombination signal probes.
In order to isolate cDNA clones for DNA-binding components of the V-(D)-J recombinase, phage libraries from a pre-B-cell line were screened with a radiolabeled probe containing recombination signal sequences (RSS). Among prospective clones, cDNA T160 was analyzed further. It produced a protein of 80.6 kDa which bound to DNA containing RSS but not to DNA in which the RSS had been mutated. A search of a data base revealed that the T160 protein has significant sequence homology (56%) to the nonhistone chromosomal protein HMG1 within the C-terminal region of 80 amino acids. DNA-binding analysis with truncated proteins showed that the HMG homology region is responsible for DNA binding. Using restriction fragment length polymorphisms, the T160 gene was mapped at the proximal end of mouse chromosome 2. Evidence was obtained for genetic linkage between the T160 gene and the recombination activator genes RAG-1 and RAG-2.
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
M
M
Shirakata
K
K
Hüppi
S
S
Usuda
K
K
Okazaki
K
K
Yoshida
H
H
Sakano
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics*; D008040:Genetic Linkage; D006609:High Mobility Group Proteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D016415:Sequence Alignment
D015342:DNA Probes; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; C070454:Ssrp1 protein, mouse; D004247:DNA