Detection of IgG, IgA, IgM and IgE antibodies in invasive amoebiasis in endemic areas.
Entamoeba histolytica-specific serum IgG, IgA, IgM and IgE antibodies were assayed in cases of amoebiasis in an endemic area. Patient groups consisted of amoebic liver abscess (n = 18), preabscess hepatic amoebiasis (n = 22) and amoebic colitis (n = 30). Control subjects comprised 26 asymptomatic cyst passers, 13 giardiasis cases, 20 typhoid patients and 24 non-amoebic individuals. Serum IgG was assayed by ELISA, using a monoclonal anti IgG beta-galactosidase (IgG beta-gal) conjugate, a polyclonal avidin biotin horse radish peroxidase (AB-HRP), and a polyclonal anti IgG horse radish peroxidase (IgG HRP) conjugate. IgA and IgM were assayed by the beta-gal ELISA and IgE by AB-HRP. Diagnostically significant IgG and IgA while lower IgM and IgE antibody levels were seen in extraintestinal cases. About 40% of suspected pre-abscess hepatic amoebiasis cases were confirmed by antibody estimation. All isotype levels in most dysentery cases were in the range of the controls.
Shetty
N
N
Department of Microbiology, St. John's Medical College, Bangalore, India.
Nagpal
S
S
Rao
P V
PV
Schröder
H
H
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D004404:Dysentery, Amebic / Q000097:blood / Q000453:epidemiology; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000097:blood / Q000453:epidemiology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005243:Feces / Q000469:parasitology; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis* / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis* / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology; D008101:Liver Abscess, Amebic / Q000097:blood / Q000453:epidemiology; D016036:Seroepidemiologic Studies
D000913:Antibodies, Protozoan; D000939:Epitopes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007073:Immunoglobulin E