Intracerebral grafting of fetal dorsal pons in genetically epilepsy-prone rats: effects on audiogenic-induced seizures.
The present experiments investigated if dorsal pontine tissue obtained from 16-day postconception rat fetuses and stereotaxically transplanted into the dorsal hippocampus or third ventricle of genetically epilepsy-prone rats (GEPRs) would alter the expression of audiogenic seizures. Of eight GEPR-9s receiving pontine-tissue grafts bilaterally into the dorsal hippocampus, none showed any reduction in AGS severity. In contrast, three of five GEPR-9s receiving grafts into the third ventricle eventually displayed a decreased seizure severity following transplantation. Of five GEPR-3s receiving transplants into the hippocampus, one animal showed a gradual and significant reduction in seizure severity after transplantation. Tyrosine-hydroxylase (TH) immunohistochemistry showed that transplanted tissue contained abundant TH-immunoreactive profiles including perikarya and fibers. The results of these preliminary studies suggest that the GEPR model of epilepsy may be useful in studying the corrective potential of neurotransplants.
Clough
R W
RW
Department of Anatomy, Southern Illinois University School of Medicine, Carbondale 62901.
Browning
R A
RA
Maring
M L
ML
Jobe
P C
PC
D000161:Acoustic Stimulation; D000375:Aging; D000818:Animals; D016380:Brain Tissue Transplantation / Q000502:physiology*; D002552:Cerebral Ventricles / Q000254:growth & development / Q000502:physiology / Q000503:physiopathology*; D004827:Epilepsy / Q000235:genetics / Q000503:physiopathology*; D016332:Fetal Tissue Transplantation / Q000502:physiology; D005333:Fetus; D006624:Hippocampus / Q000254:growth & development / Q000502:physiology / Q000503:physiopathology*; D011149:Pons / Q000637:transplantation*; D051381:Rats; D011922:Rats, Mutant Strains; D012640:Seizures / Q000503:physiopathology*; D013997:Time Factors; D016042:Transplantation, Heterotopic; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D014446:Tyrosine 3-Monooxygenase