Chicken egg antibodies for prophylaxis and therapy of infectious intestinal diseases. IV. In vitro studies on protective effects against adhesion of enterotoxogenic Escherichia coli to isolated enterocytes.
The pathogenic effect of enterotoxogenic E. coli is mainly to be found in the jejunum. Hence, an effective immunoprophylaxis should be focussed at this site. This can be done effectively by an oral application of specific antibodies. The present study describes the effectiveness of specific antibodies against E. coli. The antibodies were gained from the yolk of E. coli immunized hens and tested for their in vitro activity. The tests showed a significantly reduced adhesion of enterotoxogenic E. coli onto isolated pig enterocytes as long as the bacteria had been incubated with the specific yolk antibodies beforehand. For this reason, yolk immunoglobulins from immunized hens prove to be an effective and handy source of specific antibodies which offers good protection against adhesion of E. coli onto pig enterocytes.
Jüngling
A
A
Institut für Physiologie, Physiologische Chemie und Ernährungsphysiologie, Tierärztliche Fakultät, Universität München, FRG.
Wiedemann
V
V
Kühlmann
R
R
Erhard
M
M
Schmidt
P
P
Lösch
U
U
D018830:Adhesins, Escherichia coli; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002478:Cells, Cultured; D002645:Chickens; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D007422:Intestines / Q000166:cytology / Q000382:microbiology*; D013552:Swine
D018830:Adhesins, Escherichia coli; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins