Electrochemical detection of human brain transmitter amino acids by high-performance liquid chromatography of stable o-phthalaldehyde-sulphite derivatives.
A simple, sensitive, reliable and reproducible isocratic HPLC technique for the measurement of OPA/sulphite derivatives of human brain amino acid neurotransmitters is described. This employs a sample preparation that is also compatible with the concurrent determination of monoamines and their metabolites on a separate HPLC system. The method has been applied to the determination of GABA and glutamate in brain tissue taken post-mortem from patients with Huntington's disease and control subjects.
Pearson
S J
SJ
Department of Biomedical Science, University of Sheffield, United Kingdom.
Czudek
C
C
Mercer
K
K
Reynolds
G P
GP
D000596:Amino Acids / Q000032:analysis*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D006816:Huntington Disease / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000032:analysis*; D013447:Sulfites; D009764:o-Phthalaldehyde
D000596:Amino Acids; D018377:Neurotransmitter Agents; D013447:Sulfites; D009764:o-Phthalaldehyde; C025026:sodium sulfite