Survival of developing dopaminergic neurons in reaggregate tissue culture following treatment with methamphetamine.
Developing central dopamine (DA)-containing neurons in rotation-mediated reaggregate tissue culture exposed to 10(-4) M methamphetamine for 1 week (at a time corresponding to the 2nd week of postnatal development) exhibit decreased DA levels. DA neurons were visualized either by a histofluorescent method after 'loading' with exogenous DA, or by tyrosine hydroxylase (TH) immunocytochemistry. An apparent loss of histofluorescent DA cell bodies was observed due to a methamphetamine-induced reduction in exogenous DA accumulation. No decrease in the number of TH-immunoreactive neurons was observed. Thus, developing DA neurons when visualized by TH immunocytochemistry survive despite methamphetamine-induced reductions in DA levels.
Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637.
P J
PJ
Kontur
L A
LA
Won
P C
PC
Hoffmann
A
A
Heller
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D046508:Culture Techniques; D004298:Dopamine / Q000378:metabolism / Q000502:physiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008694:Methamphetamine / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D011247:Pregnancy; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D008694:Methamphetamine; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine