Target sequences for hunchback in a control region conferring Ultrabithorax expression boundaries.
Boundaries of Ultrabithorax expression are mediated by long-range repression acting through the PBX or ABX control region. We show here that either of these control regions confers an early band of beta-galactosidase expression which is restricted along the anteroposterior axis of the blastoderm embryo. This band is succeeded by a stripe pattern with very similar anteroposterior limits. Dissection of the PBX control region demonstrates that the two patterns are conferred by distinct cis-regulatory sequences contained within separate PBX subfragments. We find several binding sites for hunchback protein within both PBX subfragments. Zygotic hunchback function is required to prevent ectopic PBX expression. Moreover, the PBX pattern is completely suppressed in embryos containing uniformly distributed maternal hunchback protein. Our results strongly suggest that hunchback protein directly binds to the PBX control region and acts as a repressor to specify the boundary positions of the PBX pattern.
Zhang
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CC
Zoological Institute, University of Zürich, Switzerland.
Müller
J
J
Hoch
M
M
Jäckle
H
H
Bienz
M
M
D000818:Animals; D001483:Base Sequence; D001756:Blastoderm / Q000201:enzymology / Q000502:physiology*; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D005801:Genes, Homeobox / Q000235:genetics*; D007150:Immunohistochemistry; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase