Beta-adrenergic blockade and lipoprotein lipase activity in rat tissues after acute exercise.
The present experiments were aimed at evaluating the acute effects of exercise on lipoprotein lipase (LPL) activity in untrained rats. The activity of LPL was measured in postheparin plasma (PHP) before and at various times after a 1-h run on a treadmill (22 m/min, O degrees grade). LPL in PHP was 50% below pre-exercise levels immediately and 3 h after the run but was increased 65% over resting levels 24 h postexercise. To further characterize the very early fall in LPL activity in response to exercise and to assess the possible involvement therein of the beta-adrenergic pathway, LPL in heart, vastus lateralis muscle (VLM), and white (WAT) and brown (BAT) adipose tissues was determined at rest and immediately after exercise in rats that were treated or not with nadolol (25 mg.kg-1.day-1 for 30 days). Immediately after 1 h of exercise, there was a reduction in total enzyme activity in WAT (40% below resting levels), BAT (-58%), VLM (-53%), and heart (-30%). Exercise reduced serum triacylglycerol levels (-64%) and doubled those of nonesterified fatty acids. beta-Adrenergic blockade did not affect any of these variables. Both exercise and nadolol lowered serum cholesterol levels by approximately 20%, but the effects were not additive. These results show that the global intravascular pool of LPL undergoes divergent, time-dependent alterations in response to a single bout of moderate exercise. The acute downregulation of postheparin plasma LPL immediately after exercise reflected a fall in the total enzyme pool of all tissues studied.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Physiology, School of Medicine, Laval University, Quebec, Canada.
A
A
Paulin
J
J
Lalonde
Y
Y
Deshaies
D000273:Adipose Tissue / Q000033:anatomy & histology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D006321:Heart / Q000033:anatomy & histology; D006339:Heart Rate / Q000187:drug effects; D007545:Isoproterenol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008055:Lipids / Q000097:blood; D008071:Lipoprotein Lipase / Q000097:blood*; D008297:Male; D009132:Muscles / Q000033:anatomy & histology; D009248:Nadolol / Q000494:pharmacology; D009929:Organ Size / Q000187:drug effects; D005082:Physical Exertion; D010865:Pilot Projects; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000319:Adrenergic beta-Antagonists; D008055:Lipids; D009248:Nadolol; D008071:Lipoprotein Lipase; D007545:Isoproterenol