Transcription of the baculovirus polyhedrin gene reduces the levels of an antisense transcript initiated downstream.
A late 3.2-kilobase (kb) RNA initiated approximately 2 kb downstream of the 3' end of the Autographa californica nuclear polyhedrosis virus polyhedrin-coding sequence and traversed the polyhedrin gene in an antisense direction. This RNA was sense RNA for the two open reading frames flanking the polyhedrin gene. A mutant virus, vXpoly, which differs from wild-type virus only at the essential RNA initiation site in the polyhedrin promoter, exhibited higher levels of the 3.2-kb RNA than did wild-type virus during the polyhedrin transcriptional phase. Thus, transcription of the polyhedrin gene down regulates the levels of this 3.2-kb RNA.
Ooi
B G
BG
Department of Entomology, University of Georgia, Athens 30602.
Miller
L K
LK
D000818:Animals; D015152:Blotting, Northern; D015967:Gene Expression Regulation, Viral; D005809:Genes, Regulator; D005814:Genes, Viral; D007304:Insect Viruses / Q000235:genetics*; D009710:Nucleotide Mapping; D000077183:Occlusion Body Matrix Proteins; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000037:antagonists & inhibitors*; D012367:RNA, Viral / Q000235:genetics*; D015183:Restriction Mapping; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
D000077183:Occlusion Body Matrix Proteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus; D012313:RNA; D015719:Single-Strand Specific DNA and RNA Endonucleases