Cyclic nucleotide-activated channels in the frog olfactory receptor plasma membrane.
Patch clamp technique was used to record cyclic nucleotide-dependent current of the frog olfactory receptor cell plasma membrane. Data obtained indicate that the channels passing this current are permeable to Ca2+ or Mg2+ and moderately selective for monovalent cations according to the sequence Li+, Na+, K+ greater than Rb+ greater than Cs+ and are effectively blocked by 1-cis-diltiazem and 3',4'-dichlorobenzamil. The conductance of single cyclic nucleotide-gated channels in solutions with low Ca2+ and Mg2+ content is about 19 pS. The results demonstrate that cyclic nucleotide-activated channels of olfactory receptor cells are virtually identical to photoreceptor ones.
Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region.
S S
SS
Kolesnikov
A B
AB
Zhainazarov
A V
AV
Kosolapov
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002413:Cations, Divalent / Q000494:pharmacology; D002414:Cations, Monovalent / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology; D004553:Electric Conductivity; D006160:Guanosine Triphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D009712:Nucleotides, Cyclic / Q000502:physiology*; D009831:Olfactory Mucosa / Q000502:physiology*; D011898:Ranidae / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000502:physiology*; D012903:Smell / Q000502:physiology*
D002413:Cations, Divalent; D002414:Cations, Monovalent; D007473:Ion Channels; D009712:Nucleotides, Cyclic; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate