[Methods of studying genes and gene function].
In several fields, such as immunology, biochemistry, and structural biology, but particularly in molecular genetics, modern methods enable genes and their protein products to be analysed and described. The tools are now available to study any physiological process or disease at the molecular level. As a result, genetic diseases and viral infections can be better identified and understood. Basic cellular and physiological mechanisms are beginning to be elucidated, the disturbance of which is a factor involved in cancerogenesis, for instance, or the development of hypercholesterolaemia. The article is intended as a brief review of current methods of studying genes and their function. The isolation and characterisation of genes by means of recombinant DNA technology, the identification of defective genes, and the analysis of gene function, both in vitro and in vivo are presented.
Institutionen för molekylär genetik, Karolinska institutet, Stockholm.
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Wieslander
D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D015723:Gene Library; D006801:Humans; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics
D004274:DNA, Recombinant; D012313:RNA; D004247:DNA
Metoder att studera gener och geners funktion.