Choline derivatives increase two different acid phosphatases in Rhizobium meliloti and Pseudomonas aeruginosa.
In Pseudomonas aeruginosa and Rhizobium meliloti several choline derivatives, utilized as the sole carbon and nitrogen source, increase acid phosphatase activity. The enzyme activity of both bacteria could be released into the surrounding medium by EDTA-lysozyme treatment. The R. meliloti acid phosphatase activity of crude periplasmic extracts measured with p-nitrophenylphosphate was not inhibited by the presence of 5 mM choline, betaine, trimethylammonium or phosphorylcholine. The activity could not be detected using phosphorylethanolamine or phosphorylcholine as substrates. Among several phosphoesters tested only pyridoxal-5-phosphate was hydrolyzed at a considerable rate. In 7.5% polyacrylamide slab gel electrophoresis (non-denaturing conditions) of crude extracts obtained from bacteria grown in the presence of serine, glutamate, aspartate or dimethylglycine a phosphatase activity with identical mobility could be detected with alpha-naphthylphosphate or pyridoxal-5-phosphate were used as substrates. In conclusion, although the choline metabolites are capable of increasing acid phosphatase activities in R. meliloti and in P. aeruginosa, there are two different enzymes involved, apparently in different metabolisms.
Departmento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
A E
AE
Lucchini
T A
TA
Lisa
C E
CE
Domenech
D000135:Acid Phosphatase / Q000378:metabolism*; D001285:Atropine / Q000378:metabolism / Q000494:pharmacology; D001622:Betaine / Q000378:metabolism / Q000494:pharmacology; D002794:Choline / Q000378:metabolism / Q000494:pharmacology*; D003470:Culture Media; D010767:Phosphorylcholine / Q000378:metabolism / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism / Q000494:pharmacology; D012231:Rhizobium / Q000187:drug effects / Q000201:enzymology*; D012521:Sarcosine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology
D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D010767:Phosphorylcholine; D001622:Betaine; C025138:dimethylglycine; D001285:Atropine; D000135:Acid Phosphatase; D002794:Choline; D012521:Sarcosine