Thy-1 antigen is specific to ganglion cells in chicks.
The cellular localization of Thy-1 in the chick retina was investigated by selectively destroying certain populations of neurons with toxins. In control retinae four weeks after intravitreal injection of vehicle, there was strong immunoreactivity for Thy-1 in the nerve fibre layer, ganglion cell layer and inner plexiform layer. By contrast, 4 weeks after intraocular injection with 1.25 nmol of colchicine, virtually all ganglion cells had been destroyed, but most amacrine cells remained. Very little Thy-1 immunoreactivity was evident in these retinae. Four weeks after intraocular injection of 2 mumol of N-methyl-D-aspartic acid (NMDA), a large proportion of amacrine cells had been destroyed, but most ganglion cells remained. In these retinae Thy-1 immunoreactivity was present in the nerve fibre, ganglion cell and inner plexiform layers, in the latter with greater intensity than in controls. We conclude that in chicks the Thy-1 antigen is principally, if not exclusively restricted to ganglion cells.
Children's Medical Research Foundation, University of Sydney, Camperdown N.S.W. Australia.
A M
AM
Sheppard
M
M
Konopka
S R
SR
Robinson
I G
IG
Morgan
P L
PL
Jeffrey
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002645:Chickens; D003078:Colchicine / Q000494:pharmacology; D005260:Female; D007124:Immunoenzyme Techniques; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology; D009412:Nerve Fibers / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure; D012160:Retina / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D018800:Thy-1 Antigens; D016202:N-Methylaspartate; D003078:Colchicine