Comparison of four stains and a urease test for rapid detection of Helicobacter pylori in gastric biopsies.
Gastric biopsies were obtained from 125 subjects to compare detection of Helicobacter pylori by culture, a rapid urease test and histopathologic examination using haematoxylin-eosin, Gram, Giemsa, Warthin-Starry silver and acridine orange stains. Helicobacter pylori was isolated from 39 specimens. Acridine orange and Giemsa were the most sensitive stains, detecting 85% and 79% of positive specimens respectively. All stains showed high specificity (97-100%). The sensitivity and specificity of the rapid urease test was 62% and 100% respectively. These stains or the rapid urease test may be useful for rapid detection of Helicobacter pylori in gastric biopsies.
Department of Microbiology, Mount Sinai Hospital, University of Toronto, Ontario, Canada.
A E
AE
Simor
N B
NB
Cooter
D E
DE
Low
D001706:Biopsy; D016009:Chi-Square Distribution; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology / Q000302:isolation & purification*; D006801:Humans; D011237:Predictive Value of Tests; D011706:Pyloric Antrum / Q000382:microbiology*; D013194:Staining and Labeling; D014510:Urease / Q000378:metabolism*
D014510:Urease