BCR rearrangements in Ph positive chronic myelocytic leukemia.
Nine patients with chronic myelocytic leukemia (CML) and 1 patient with erythroleukemia were studied with 3'bcr and 5'bcr probes using Southern blot hybridization technique. Bcr rearrangements were detected in 8 patients with CML in the chronic phase, and bcr rearrangement was deduced to have existed in a CML patient in blastic crisis. However, no abnormal fragment was found in the patient with erythroleukemia. 3'bcr and 5'bcr probes combined with proper restriction enzymes were believed to be of great value in determining bcr rearrangements in Ph positive CML.
Deng
D S
DS
Institute of Hematology, Union Hospital Tongj i Medical University, Wuhan.
Yang
A D
AD
Gong
W L
WL
Fei
H B
HB
Liu
S M
SM
D000293:Adolescent; D000328:Adult; D001752:Blast Crisis / Q000235:genetics; D015139:Blotting, Southern; D002675:Child, Preschool; D015342:DNA Probes; D005260:Female; D015321:Gene Rearrangement; D016313:Genes, abl; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000473:pathology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes