Hydrolysis of [Leu]enkephalin by chick brain in vitro.
Using high-performance liquid chromatography with electrochemical detection to measure substrate disappearance and metabolite accumulation following addition of [Leu]enkephalin to samples prepared from chick brain in vitro, the following were found: 1. [Leu]enkephalin hydrolysis by whole forebrain homogenates is almost solely attributable to aminopeptidase MII activity. 2. [Leu]enkephalin hydrolysis by whole forebrain P2 membrane fractions is attributable to both aminopeptidase MII and dipeptidyl carboxypeptidase activity. 3. Differences are apparent in both [Leu]enkephalin disappearance and Tyr-Gly-Gly accumulation in P2 membrane fractions, but not in homogenate fractions, prepared from several regions of the chick brain.
Department of Pharmacology, Nihon University School of Medicine, Tokyo, Japan.
S
S
Shibanoki
S B
SB
Weinberger
D
D
Beniston
G
G
Schulteis
E L
EL
Bennett
M R
MR
Rosenzweig
K
K
Ishikawa
J L
JL
Martinez
D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D018826:CD13 Antigens; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D010450:Endopeptidases / Q000378:metabolism; D004743:Enkephalin, Leucine / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D009928:Organ Specificity
D004743:Enkephalin, Leucine; D010450:Endopeptidases; D000626:Aminopeptidases; D018826:CD13 Antigens; C020071:dipeptidyl carboxypeptidase