Expression of messenger RNAs for peptides and tyrosine hydroxylase in primary sensory neurons that innervate arterial baroreceptors and chemoreceptors.
Retrograde fiber tracing and in situ hybridization were used to determine expression of mRNAs for preprotachykinin A (ppTA), calcitonin gene related peptide (CGRP), preproenkephalin A (ENK), neuropeptide tyrosine (NPY) and somatostatin (SOM) as well as tyrosine hydroxylase (TH) in the petrosal ganglia primary sensory neurons which innervate carotid sinus baroreceptors and carotid body chemoreceptors. Perfusion of the carotid sinus with the retrogradely transported dye (Fluoro-Gold) labeled primary sensory neurons in petrosal ganglion. Numerous somata in the petrosal ganglion labeled with dye contained mRNAs for all the above peptides, except SOM. Moreover, TH mRNA was found in a substantial number of retrogradely labeled cells in the petrosal ganglion. This study provides information concerning which of the numerous peptides identified in sensory neurons of petrosal ganglion may be involved in modulation of the arterial baroreceptor and chemoreceptor reflexes.
Czyzyk-Krzeska
M F
MF
Department of Physiology, University of North Carolina, Chapel Hill, NC 27599.
Bayliss
D A
DA
Lawson
E E
EE
Millhorn
D E
DE
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics; D002344:Carotid Body / Q000502:physiology*; D002346:Carotid Sinus / Q000502:physiology*; D004745:Enkephalins / Q000235:genetics; D008297:Male; D009475:Neurons, Afferent / Q000737:chemistry*; D009478:Neuropeptide Y / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000235:genetics; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics*
D004745:Enkephalins; D009478:Neuropeptide Y; D011498:Protein Precursors; D012333:RNA, Messenger; D013004:Somatostatin; C033660:preproenkephalin; D014446:Tyrosine 3-Monooxygenase; D015740:Calcitonin Gene-Related Peptide