Establishment of a foreign antigen secretion system in mycobacteria.
In order to develop recombinant Mycobacterium bovis BCG into a useful multivaccine vehicle, we established a foreign antigen secretion system in mycobacteria in which an extracellular alpha antigen of Mycobacterium kansasii was utilized as a carrier. By using this system, a B-cell epitope (Glu-12-Leu-Asp-Arg-Trp-Glu-Lys-Ile-19) of human immunodeficiency virus type 1 p17gag, which was identified by a fusion protein-based method, has been successfully obtained from BCG along with the alpha antigen. This is the first report of expression and secretion of a foreign viral antigen from BCG. It is possible that the system can become a universal vaccination vehicle applicable to protection against various infectious diseases.
Central Research Laboratories, Ajinomoto Co. Inc., Kawasaki City, Japan.
K
K
Matsuo
R
R
Yamaguchi
A
A
Yamazaki
H
H
Tasaka
K
K
Terasaka
M
M
Totsuka
K
K
Kobayashi
H
H
Yukitake
T
T
Yamada
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000276:immunology; D000939:Epitopes; D015683:Gene Products, gag / Q000096:biosynthesis* / Q000276:immunology; D005822:Genetic Vectors; D015488:HIV Antigens / Q000096:biosynthesis* / Q000276:immunology; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000276:immunology*; D010957:Plasmids; D014614:Vaccines, Synthetic / Q000096:biosynthesis*; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
D000939:Epitopes; D015683:Gene Products, gag; D015488:HIV Antigens; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1