Imbalance of CD4+CD45R+ and CD4+CD29+ T helper cell subsets in patients with atopic diseases.
To evaluate the proportion of helper cell subsets we studied 18 children with atopic dermatitis, 30 patients with asthma, 27 healthy age-matched controls aged 1 to 17 years and 11 atopic controls without symptoms related to atopy, aged 9-22 years. Lymphocytes were isolated from heparinized peripheral blood and the proportion of CD4+CD29+ and CD4+CD45R+ cells was determined by double-labelling immunofluorescence. Children with atopic dermatitis yielded a significantly (P less than 0.01) higher proportion of CD4+CD45R+ (median 75%) cells compared with normal controls (median 66.6%), whereas the proportion of CD4+CD29+ cells was significantly (P less than 0.01) lower in patients with atopic dermatitis (median 20.4 versus 29.6%). Interestingly, the percentage of CD4+CD45R+ cells shows an age-dependent decline (r = -0.67, P less than 0.01) in the control group, which is not found in the patient group.
Department of Paediatrics, Philipps University of Marburg, West Germany.
U
U
Schauer
T
T
Jung
J
J
Heymanns
C H
CH
Rieger
D000293:Adolescent; D000375:Aging / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D000943:Antigens, Differentiation / Q000276:immunology; D001249:Asthma / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000276:immunology*; D005434:Flow Cytometry; D006649:Histocompatibility Antigens / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D007223:Infant; D019012:Integrin beta1; D017493:Leukocyte Common Antigens; D007958:Leukocyte Count; D016176:T-Lymphocyte Subsets / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015704:CD4 Antigens; D006649:Histocompatibility Antigens; D019012:Integrin beta1; D007073:Immunoglobulin E; D017493:Leukocyte Common Antigens