Immunoblot analyses of Brazilian pemphigus foliaceus antigen using different antigen sources.
We investigated the Brazilian pemphigus foliaceus (BPf) antigen applying the immunoblotting method to two different antigen sources using 27 patients' sera. Twelve BPf sera reacted specifically with a 150 kD protein in extract of dispase separated human epidermis, while 18 sera yielded a similar protein band in bovine muzzle desmosomal preparation. The diversity of staining intensities between the two samples suggested the heterogeneity of BPf antigens in terms of epitopes. Japanese sporadic pemphigus foliaceus (Pf) sera showed similar results but Japanese pemphigus vulgaris (Pv) sera recognized different antigens of 130 kD or 135 kD, suggesting that BPf is similar to Japanese Pf but is distinct from Pv in respect to the antigenic substance. Furthermore, the present study showed that immunoblot analysis using different antigen sources should be a valuable tool to determine clinical types of pemphigus.
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
M M
MM
Ogawa
T
T
Hashimoto
A
A
Konohana
R M
RM
Castro
T
T
Nishikawa
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001938:Brazil; D002417:Cattle; D002457:Cell Extracts / Q000032:analysis / Q000276:immunology; D003896:Desmosomes / Q000032:analysis / Q000276:immunology; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D000078404:Epidermal Cells; D004817:Epidermis / Q000032:analysis / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D007564:Japan; D010392:Pemphigus / Q000175:diagnosis / Q000276:immunology*
D000941:Antigens; D002457:Cell Extracts; D000939:Epitopes; D007106:Immune Sera; D004492:Edetic Acid