Production and characterization of antibodies to mouse scrapie-amyloid protein elicited by non-carrier linked synthetic peptide immunogens.
Two polyclonal antibodies were raised by immunizing rabbits with two non carrier-linked synthetic peptides whose amino acid sequences corresponded to codons 89-107 (peptide P1) and 219-233 (peptide P2) of the translated cDNA sequence of murine PrP protein. These free peptides, whose structural characteristics in solution were studied by circular dichroism, elicited a reasonable immunologic response in animals. Both antibodies still recognized the corresponding immunogens after affinity chromatography purification. However, only antibodies raised to the former sequence reacted by immunoblot with a purified preparation of murine scrapie amyloid protein. These findings are discussed together with their correlation to peptide structure and the effectiveness of this simplified immunization procedure.
Di Martino
A
A
Advanced Technology Division, Fidia SpA, Abano Terme, Italy.
Bigon
E
E
Corona
G
G
Callegaro
L
L
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D007114:Immunization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D018620:PrPSc Proteins; D011328:Prions / Q000276:immunology*; D011817:Rabbits
D000914:Antibodies, Viral; D000956:Antigens, Viral; D010455:Peptides; D018620:PrPSc Proteins; D011328:Prions