Applications of automated amino acid analysis using 9-fluorenylmethyl chloroformate.
A rapid and sensitive fully automated method for the determination of primary and secondary amino acids in different matrices is described. Amino acids are derivatized with 9-fluorenylmethyl chloroformate using an automated precolumn derivatization technique. Data are presented to show that the technique is both reproducible and highly sensitive. Applications of the technique are presented, including the analysis of peptide and protein hydrolysates and the profiling of free amino acids in physiological fluids.
School of Chemistry, Macquarie University, NSW, Australia.
P A
PA
Haynes
D
D
Sheumack
L G
LG
Greig
J
J
Kibby
J W
JW
Redmond
D000328:Adult; D000592:Amino Acid Metabolism, Inborn Errors / Q000097:blood; D000596:Amino Acids / Q000032:analysis* / Q000097:blood; D001322:Autoanalysis / Q000379:methods*; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005449:Fluorenes; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008832:Microchemistry; D010661:Phenylketonurias / Q000097:blood; D011786:Quality Control; D014443:Tyrosine / Q000097:blood; D014444:Tyrosine Transaminase / Q000172:deficiency
D000596:Amino Acids; D005449:Fluorenes; D007202:Indicators and Reagents; D014443:Tyrosine; C054007:1-(9-fluorenyl)methyl chloroformate; D014444:Tyrosine Transaminase