[The quantitative analysis of lipopolysaccharide antigen in the blood serum of patients with salmonellosis by using complement-bound liposome lysis].
Titration of group B Salmonella O-antigen in the blood sera of patients and donors was carried out by means of the complement-dependent lysis of liposomes sensitized with S. typhimurium LPS. Good correlation (r = 0.95) of the levels of S. typhimurium somatic O-antigen in the patients' sera determined by liposomal immunoassay and aggregate hemagglutination test was established. The concentration of the antigens in the tested samples was within 0.5-50 micrograms/ml. Statistical analysis of the results obtained by liposomal immunoassay techniques demonstrated differences in the distribution functions for the blood sera of patients with different diseases and of donors.
Iarkov
S P
SP
Skopinskaia
S N
SN
Rubtsov
I V
IV
L'vova
O M
OM
Miliutina
L N
LN
D000293:Adolescent; D000942:Antigens, Bacterial / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D000939:Epitopes / Q000097:blood; D006801:Humans; D007158:Immunologic Techniques; D007223:Infant; D007251:Influenza, Human / Q000276:immunology; D008070:Lipopolysaccharides / Q000097:blood*; D008081:Liposomes; D019081:O Antigens; D011014:Pneumonia / Q000276:immunology; D012480:Salmonella Infections / Q000175:diagnosis / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology*
D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D008081:Liposomes; D019081:O Antigens
Kolichestvennyĭ analiz lipopolisakharidnogo antigena v syvorotke krovi bol'nykh sal'monellezom s pomoshch'iu komplementzavisimogo lizisa liposom.