Different tissue distribution and hormonal regulation of messenger RNAs encoding rat insulin-like growth factor-binding proteins-1 and -2.
The insulin-like growth factor-binding proteins IGFBP-1 and IGFBP-2 are low mol wt IGFBPs that are similar in structure. They are not glycosylated and have a homologous amino acid sequence, including the number and position of 18 cysteine residues and a carboxyl-terminal Arg-Gly-Asp sequence that can be recognized by cell adhesion receptors. The present study demonstrates that expression of mRNAs encoding the two BPs differs in some fetal rat tissues and in the livers of adult rats after hypophysectomy, fasting, or streptozotocin-induced diabetes. As determined by Northern blot hybridization using cDNA probes for rat IGFBP-2 or human IGFBP-1, both mRNAs are expressed at high levels in liver of 21-day gestation and 1-day-old rats and at lower levels in 21- and 65-day-old rat liver. Levels of both mRNAs are higher in liver than in other fetal rat tissues. The relative abundance of the two mRNAs in most fetal tissues is similar to that in liver, except that kidney and brain have 8-fold and more than 25-fold higher relative levels of IGFBP-2 mRNA, respectively. IGFBP-2 mRNA is about 10- to 20-fold increased after hypophysectomy or fasting, whereas IGFBP-1 mRNA is relatively unchanged. IGFBP-2 mRNA levels are decreased completely by refeeding fasted rats for 3 days, but only partially decreased by treatment of hypophysectomized rats with GH, cortisone acetate, T4, and testosterone for 4 days.(ABSTRACT TRUNCATED AT 250 WORDS)
Molecular, Cellular and Nutritional Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
G T
GT
Ooi
C C
CC
Orlowski
A L
AL
Brown
R E
RE
Becker
T G
TG
Unterman
M M
MM
Rechler
D000375:Aging / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D003348:Cortisone / Q000031:analogs & derivatives / Q000494:pharmacology; D015342:DNA Probes; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D005215:Fasting / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D013006:Growth Hormone / Q000494:pharmacology; D007016:Hypophysectomy; D018969:Insulin-Like Growth Factor Binding Proteins; D008099:Liver / Q000378:metabolism; D008297:Male; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013256:Steroids / Q000494:pharmacology*; D013739:Testosterone / Q000494:pharmacology; D013974:Thyroxine / Q000494:pharmacology; D014018:Tissue Distribution
D002352:Carrier Proteins; D015342:DNA Probes; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D013256:Steroids; D013739:Testosterone; D013006:Growth Hormone; D013974:Thyroxine; D003348:Cortisone