Oligoclonal bands in cerebrospinal fluid detected by PhastSystem isoelectric focusing.
Pharmacia's "PhastSystem" for semi-automated isoelectric focusing (IEF) in thin precast polyacrylamide gels (PAGE) was found to be as sensitive as high-resolution protein electrophoresis (HRPE) in agarose gels and conventional PAGE-IEF for detection of oligoclonal banding (OB) in concentrated cerebrospinal fluid (CSF) samples. Both PhastSystem IEF and HRPE revealed OB in CSF from eight of nine multiple sclerosis patients and four of 10 patients with various types of infection of the central nervous system as opposed to only two of 70 patients with miscellaneous neuropsychiatric disorders. The PhastSystem also frequently detected OB in silver-stained, unconcentrated CSF from patients with multiple sclerosis.
Wybo
I
I
Department of Clinical Chemistry, Academic Hospital, Vrije Universiteit Brussel, Belgium.
Van Blerk
M
M
Malfait
R
R
Goubert
P
P
Gorus
F
F
D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D003711:Demyelinating Diseases / Q000134:cerebrospinal fluid / Q000175:diagnosis; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007136:Immunoglobulins / Q000134:cerebrospinal fluid*; D007525:Isoelectric Focusing; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000175:diagnosis; D043025:Oligoclonal Bands; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D005719:gamma-Globulins / Q000134:cerebrospinal fluid*
D002556:Cerebrospinal Fluid Proteins; D007136:Immunoglobulins; D043025:Oligoclonal Bands; D012394:Rosaniline Dyes; D005719:gamma-Globulins; C004692:coomassie Brilliant Blue