Absorption of iron from iron succinyl-protein complexes by mouse small intestine.
The absorption of iron from iron succinyl-protein complexes was investigated in mice. 59Fe-labelled succinyl-casein and -albumin complexes, [59Fe]ferritin and 59FeSO4, at doses of 20 or 200 micrograms of iron, were administered orally to normal mice or mice with absorption enhanced by chronic hypoxia. 59Fe from iron succinyl-protein was well absorbed in normal mice (greater than 10% of dose) and showed enhanced absorption in hypoxic mice (greater than 40% of dose). Intestinal uptake was predominantly by the duodenum for all compounds studied. In-vivo absorption of 59Fe from an iron succinyl-protein complex was studied using tied-off segments of mouse duodenum, jejunum or ileum of normal or hypoxic mice. Incubation for up to 15 min in duodenum or 60 min in ileum showed very little absorption of 59Fe. No enhancement of absorption was seen in hypoxic mice. It was concluded that absorption of the intact iron succinyl-protein complex cannot explain absorption seen after oral dosing.
Simpson
R J
RJ
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, London, UK.
Raja
K B
KB
Peruzzi
M
M
Cremonesi
P
P
D000818:Animals; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D007501:Iron / Q000493:pharmacokinetics*; D007504:Iron Radioisotopes; D008667:Metalloproteins / Q000493:pharmacokinetics*; D051379:Mice; D013386:Succinates / Q000493:pharmacokinetics*
D007504:Iron Radioisotopes; D008667:Metalloproteins; D013386:Succinates; D007501:Iron; C055066:iron protein succinylate