Ischemia reperfusion injury and histamine release in isolated and perfused guinea-pig heart: pharmacological interventions.
Experiments were carried out to provide further information on the biochemical and morphological changes occurring in the guinea-pig heart after multiple ligature and reopening of the left anterior descending (LAD) coronary artery. In isolated perfused guinea-pig heart the reopening of LAD coronary artery leads to a release of histamine related to a loss of metachromasia by cardiac mast cells. The process is associated with malonyldialdehyde (MDA) production, cellular overload of calcium and ventricular arrhythmias which can be modulated by pharmacological interventions.
Masini
E
E
Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.
Bianchi
S
S
Gambassi
F
F
Palmerani
B
B
Pistelli
A
A
Carlomagno
L
L
Mannaioni
P F
PF
D000818:Animals; D003331:Coronary Vessels / Q000502:physiology; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects / Q000503:physiopathology*; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*; D009206:Myocardium / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*
D007770:L-Lactate Dehydrogenase