Detection of HIV in the peripheral mononuclear cells of asymptomatic haemophiliacs in Hungary.
The presence of virus in peripheral blood mononuclear cells of asymptomatic antibody positive haemophiliacs was detected by assaying for reverse transcriptase and confirmed by electron microscopy and immunofluorescence. HIV has been detected in 5 out of 7 individuals. In order to investigate strain variation, supernatant fluids of cultures were added to H9 and MT-4 cells. Virus was recovered in MT-4 cells in 3 cases, whereas the H9 cells only supported the replication of 2 strains. Viruses isolated from asymptomatic haemophiliacs have a narrower range of infectivity than HTLV-IIIB.
Institute of Microbiology, University Medical School, Debrecen, Hungary.
B
B
Szabó
F D
FD
Tóth
J
J
Laczkó
J
J
Kiss
E
E
Ujhelyi
G
G
Füst
Z R
ZR
Hollán
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D015497:HIV-1 / Q000201:enzymology / Q000302:isolation & purification* / Q000648:ultrastructure; D006467:Hemophilia A / Q000150:complications / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology; D006801:Humans; D006814:Hungary; D007963:Leukocytes, Mononuclear / Q000382:microbiology* / Q000648:ultrastructure; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism
D012194:RNA-Directed DNA Polymerase