Activities of dipeptidyl peptidase II, dipeptidyl peptidase IV, prolyl endopeptidase, and collagenase-like peptidase in synovial membrane from patients with rheumatoid arthritis and osteoarthritis.
We examined the activities of peptidases in the synovial membrane from patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Dipeptidyl peptidase II (DPP II), prolyl endopeptidase (PEP), and collagenase-like peptidase (CLP) activities were higher in knee joint synovial membrane from patients with RA than in that from patients with OA. DPP II and PEP activities in knee joint synovial membrane of patients with RA increased in parallel with the increase in joint fluid volume, whereas DPP IV activity decreased in parallel with the increase in joint fluid volume. These results suggest that these peptidases in the synovial membrane may play some role in immunological disturbances in the joints of patients with RA. Measurement of these peptidases in synovial membrane may be useful in the diagnosis of the severity of local joint inflammation.
Department of Orthopedic Surgery, Nagoya University School of Medicine, Japan.
M
M
Kamori
M
M
Hagihara
T
T
Nagatsu
H
H
Iwata
T
T
Miura
D000595:Amino Acid Sequence; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000201:enzymology*; D001799:Blood Sedimentation; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D005260:Female; D006801:Humans; D007719:Knee Joint; D008297:Male; D003012:Microbial Collagenase / Q000378:metabolism*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009842:Oligopeptides; D010003:Osteoarthritis / Q000097:blood / Q000201:enzymology*; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases; D013583:Synovial Membrane / Q000201:enzymology*
D009842:Oligopeptides; D010450:Endopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; C100151:dipeptidyl peptidase II; D018819:Dipeptidyl Peptidase 4; D012697:Serine Endopeptidases; C000705287:PREPL protein, human; D000085822:Prolyl Oligopeptidases; D003012:Microbial Collagenase