Use of purified F1845 fimbrial adhesin to study localization and expression of receptors for diffusely adhering Escherichia coli during enterocytic differentiation of human colon carcinoma cell lines HT-29 and Caco-2 in culture.
Whole diffusely adhering Escherichia coli (DAEC) C1845 cells bearing the F1845 adhesive factor bind diffusely to differentiated human colon carcinoma cell lines HT-29 and Caco-2. By using antibodies directed against the purified fimbrial adhesin F1845 factor, the expression of the DAEC F1845-specific brush border receptors in the polarized human intestinal HT-29 and Caco-2 epithelial cells was studied by indirect immunofluorescence. A low level of DAEC F1845 receptors in undifferentiated intestinal cells was detected; they were localized in a cluster of cells. DAEC F1845 receptors were expressed at a high level in differentiated HT-29 and Caco-2 cells. DAEC F1845 receptors were expressed at a strikingly high level in the apical domains of the cells and developed during enterocytic differentiation in culture, in parallel with the apical expression of the intestinal brush border hydrolase, sucrase-isomaltase.
Département de Microbiologie et Immunologie, UFR Sciences Pharmaceutiques Paris XI, Châtenay-Malabry, France.
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D001422:Bacterial Adhesion; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism / Q000382:microbiology; D003106:Colon / Q000166:cytology / Q000382:microbiology*; D004926:Escherichia coli / Q000378:metabolism / Q000472:pathogenicity*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D011485:Protein Binding; D011971:Receptors, Immunologic / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D011971:Receptors, Immunologic; C037872:pili, bacterial receptor