Binding of tele-methylhistamine to rat peritoneal cells.
Rat peritoneal cells were used to study the uptake of tele-methylhistamine (MeHi) from the incubation medium. The cells obtained from the peritoneal cavity were concentrated five times. MeHi in the cells was measured using an HPLC-assay with fluorescence detection. After the incubation of the cells in a buffer medium containing different concentrations of MeHi, a significant increase in MeHi content within the cell fraction was found. The increase was dose- and time-dependent. Preincubation with compound 48/80 did not affect the uptake of MeHi by the cells. The results indicate that MeHi may be taken up by mast cells.
Department of Pharmacology, Medical Faculty, Ljubljana, Yugoslavia.
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Florjanc
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Erjavec
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002851:Chromatography, High Pressure Liquid; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D006636:Histamine Release; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D008761:Methylhistamines / Q000378:metabolism*; D051381:Rats; D013050:Spectrometry, Fluorescence
D007202:Indicators and Reagents; D008761:Methylhistamines; C020404:tele-methylhistamine