The B-cells that express anti-MAG antibodies in neuropathy and non-malignant IgM monoclonal gammopathy belong to the CD5 subpopulation.
B-cells that express monoclonal IgM anti-MAG antibodies (M-protein) in a patient with neuropathy and non-malignant IgM monoclonal gammopathy were studied by flow cytometry to determine if they bear the CD5 cell surface antigen. The CD5+ antigen is expressed on a subpopulation of B-cells which has been implicated in the secretion of autoantibodies and development of B-cell chronic lymphocytic leukemia (B-CLL). The monoclonal anti-MAG antibodies secreting B-cells were identified by a mouse monoclonal antibody to M-protein idiotype. Cytofluorometric analysis revealed that approximately 62% of the idiotype-positive B-lymphocytes expressed the CD5 antigen. The result of this study indicate that monoclonal anti-MAG antibodies may be secreted by CD5+ B-cells, and that B-cells in some cases of non-malignant IgM monoclonal gammopathy may be phenotypically similar to B-CLL cells.
Department of Neurology, Columbia University, College of Physicians and Surgeons, New York, NY 10032.
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Lee
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Inghirami
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Spatz
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Knowles
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Latov
D000906:Antibodies / Q000032:analysis*; D018951:Antigens, CD20; D000943:Antigens, Differentiation / Q000032:analysis*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D001927:Brain Diseases / Q000276:immunology*; D018956:CD5 Antigens; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology; D008998:Monoclonal Gammopathy of Undetermined Significance / Q000276:immunology*; D009185:Myelin Proteins / Q000276:immunology*; D018992:Myelin-Associated Glycoprotein
D000906:Antibodies; D018951:Antigens, CD20; D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D018956:CD5 Antigens; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein