A simple method for the preparation of liposomes for pharmaceutical applications: characterization of the liposomes.
A new method for the preparation of liposomes is described that avoids the use of pharmaceutically unacceptable solvents and energy-expensive procedures such as sonication. The method is based on the initial formation of a proliposome mixture containing lipid, ethanol and water, which is converted to lipsomes by a simple dilution step. Measurements using 6-carboxyfluorescein as a marker indicate that water-soluble drugs can be trapped with extremely high efficiency (65-80% depending on lipid composition). The structural organization of the proliposome mixture and the final liposomes were characterized using electron microscopy and 31P-NMR.
Perrett
S
S
Biomolecular Sciences Division, King's College London, UK.
Golding
M
M
Williams
W P
WP
D002784:Cholesterol / Q000737:chemistry; D004530:Egg Yolk / Q000032:analysis; D005452:Fluoresceins; D007444:Inulin; D008081:Liposomes / Q000138:chemical synthesis*; D009682:Magnetic Resonance Spectroscopy; D008854:Microscopy, Electron; D010316:Particle Size; D010712:Phosphatidic Acids / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D012995:Solubility; D012997:Solvents
D005452:Fluoresceins; D008081:Liposomes; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D012997:Solvents; C024098:6-carboxyfluorescein; D007444:Inulin; D002784:Cholesterol