Gene expression and prostate specificity of human prostatic acid phosphatase (PAP): evaluation by RNA blot analyses.
A fragment of a complementary DNA (cDNA) clone for human prostatic acid phosphatase (PAP) (EC 3.1.3.2.) was used to study the expression of corresponding mRNA in human tissues. The specificity of its expression in benign prostatic hyperplasia (BPH) and prostatic carcinoma tissues were indicated in RNA blot analyses. The PAPcDNA probe did not recognize any specific mRNAs in RNAs extracted from human liver cancer, lung cancer, pancreatic cancer, placenta, breast cancer cells (MCF-7), mononuclear blood cells or acute promyelocytic leukemia cells (HL-60), according to Northern blot analysis. mRNA for PAP was detected in the androgen-dependent human prostatic cancer cell line LNCaP, but not in the androgen-insensitive human prostatic cancer cell line PC-3. In contrast, lysosomal acid phosphatase (LAP) mRNA was detected in both of these human prostatic cancer cell lines. Our findings indicate a high specificity for the PAP gene in prostatic tissue. The mean abundance for the PAPmRNA expression was 0.26 for prostatic carcinoma samples (n = 11) and 0.46 for BPH samples (n = 8) according to slot-blot analysis. The differences observed between the different categories of prostatic tissue in PAPmRNA abundances call for additional studies on regulation of its expression.
Solin
T
T
Biocenter, University of Oulu, Finland.
Kontturi
M
M
Pohlmann
R
R
Vihko
P
P
D000135:Acid Phosphatase / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D002460:Cell Line; D015342:DNA Probes; D015870:Gene Expression; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D011467:Prostate / Q000201:enzymology*; D011471:Prostatic Neoplasms / Q000201:enzymology; D012313:RNA / Q000235:genetics*; D011863:Radioimmunoassay; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured / Q000201:enzymology
D015342:DNA Probes; D012313:RNA; D000135:Acid Phosphatase