Variation in epitopes of the B subunit of Vibrio cholerae non-O1 and Vibrio mimicus cholera toxins.
Monoclonal antibodies reacting with the B subunit of Vibrio cholerae O1 strain 569B cholera toxin (CT-B) were used to identify unique and common epitopes of V. cholerae non-O1 and Vibrio mimicus CT-B. Vibrio cholerae non-O1 strains produced CT-B showing three monoclonal antibody reaction patterns (epitypes), which corresponded with epitypes described previously for V. cholerae O1 classical biotype CT-B (CT1), El Tor biotype CT-B (CT2), and a unique V. cholerae non-O1 CT-B (CT3), which lacked an epitope located in or near the GM1 ganglioside binding site of 569B CT-B. Vibrio mimicus CT-B was immunologically indistinguishable from 569B CT-B. These and previous results define six epitopes on 569B CT-B, and a fourth epitope in or near the GM1 ganglioside binding site.
Center of Marine Biotechnology, Maryland Biotechnology Institute, University of Maryland, Baltimore 21202.
M L
ML
Tamplin
R
R
Jalali
M K
MK
Ahmed
R R
RR
Colwell
D000911:Antibodies, Monoclonal; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000032:analysis; D001665:Binding Sites; D002772:Cholera Toxin / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005677:G(M1) Ganglioside / Q000378:metabolism; D014733:Vibrio / Q000378:metabolism*; D014734:Vibrio cholerae / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D005677:G(M1) Ganglioside; D002772:Cholera Toxin