A cell line (LIM 2463) derived from a tubulovillous adenoma of the rectum.
We describe a new epithelial cell line (LIM 2463) derived from tubulovillous adenoma of the rectum. The cells grow as organoids and secrete large amounts of mucus. The cells are polarized, with a microvillar brush border, and express dipeptidyl peptidase IV in a polarized manner. No staining was seen with 3 other antibodies directed against other brush-border hydrolases or disaccharidases. Focal polarized staining was obtained with 2 antibodies directed against other brush-border-associated peptides. The cells are aneuploid with a distinctive karyotype (48, XX,  +9, +13). All attempts to clone the cells in semi-solid agar or to grow them as xenografts in nude athymic mice have failed.
Whitehead
R H
RH
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, Victoria, Australia.
van Eeden
P
P
Lukeis
R E
RE
D000236:Adenoma / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D009940:Organoids / Q000648:ultrastructure; D011003:Ploidies; D012004:Rectal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4