Proteins of slow axonal transport in sciatic motoneurones of rats with streptozotocin-induced diabetes or galactosaemia.
This study examined the distribution of axonally transported tubulin and a 68 kDa polypeptide in the sciatic nerve 34 days after injection of labelled methionine into the ventral horn of the spinal cord of control rats, rats with streptozotocin-induced diabetes mellitus and rats fed a diet containing 40% galactose. The proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of pellets produced by treatment of nerve extracts with Triton X-100 followed by differential ultra-centrifugation. The most marked effect of both diabetes and galactosaemia was to reduce the amount of activity present in tubulin transported at a rate of 1.4 to 2.1 mm/day. The distribution of activity in the 68 kDa polypeptide band was not markedly affected by either of the experimental conditions. These findings, taken together with those of other studies, indicate that the polyol pathway may contribute to the development of some defects of nerve function in diabetic rats, but is uninvolved in others.
Department of Pharmacology, Medical College of St. Bartholomew's Hospital, London, U.K.
D R
DR
Tomlinson
G
G
Filliatreau
B
B
Figliomeni
R
R
Hassig
L
L
Di Giamberardino
G B
GB
Willars
D000818:Animals; D001345:Autoradiography; D001370:Axonal Transport; D002241:Carbohydrates / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D004591:Electrophoresis, Polyacrylamide Gel; D005693:Galactosemias / Q000503:physiopathology*; D007294:Inositol / Q000032:analysis; D008297:Male; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009046:Motor Neurons / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012584:Sciatic Nerve / Q000032:analysis*; D013462:Sulfur Radioisotopes; D014404:Tubulin / Q000032:analysis*
D002241:Carbohydrates; D009419:Nerve Tissue Proteins; D013462:Sulfur Radioisotopes; D014404:Tubulin; D007294:Inositol; D008715:Methionine