[Structural-dynamic and functional properties of native and modified streptokinase].
The process of streptokinase modification by a polymer and the dynamics of protein molecules and the polymer in a protein-polymer (dialdehyde dextran, DAD) conjugate were studied with the aid of luminescent methods. Conclusions were drawn on the structure of the protein-DAD conjugate and the selection of optimum conditions for the preparation of modified streptokinase. It is shown that modified streptokinase is more stable to the action of denaturating agents. The interaction between streptokinase and plasminogen activated by it and changes in the streptokinase-plasminogen complex for modified streptokinase were detected.
Pautov
V D
VD
Anufrieva
E V
EV
Anan'eva
T D
TD
Savel'eva
N V
NV
Taratina
T M
TM
Krakoviak
M G
MG
D003911:Dextrans; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D013300:Streptokinase / Q000032:analysis*; D014508:Urea
D003911:Dextrans; D007202:Indicators and Reagents; C026948:dextran dialdehyde; D014508:Urea; D013300:Streptokinase
Strukturno-dinamicheskie i funktsional'nye svoĭstva nativnoĭ i modifitsirovannoĭ streptokinazy.