Diurnal variation of the rat vas deferens contraction induced by stimulation of presynaptic nicotinic receptors and pineal function.
A rhythmic variation of maximal contraction induced by acetylcholine in the prostatic portion of rat vas deferens was tested. This contraction is due to the release of norepinephrine and ATP from sympathetic nerve terminals. Male Wistar rats (4 months old) were housed on a light/dark cycle (12 hr/12 hr, lights on at 6:00 A.M.). The diurnal variation of acetylcholine-induced contraction was determined on animals sacrificed every 3 hr during the day. The maximal contractile response shows a circadian (24:00 hr) and an ultradian (12:20 hr) rhythm. Otherwise, the sensitivity to acetylcholine (pD2 values) and the maximal contraction or pD2 values to norepinephrine, ATP and K+ did not change throughout the day. The blocking effect of hexamethonium on the contraction induced by field stimulation was higher at 9:00 P.M. than at 3:00 P.M., indicating a diurnal variation of the effect of endogenous released acetylcholine. When melatonin released by the pineal gland is suppressed by constant illumination or superior cervical ganglionectomy, the circadian rhythm was abolished and the period of the ultradian rhythm changed to 6:30 hr. The acetylcholine-induced contraction of vasa deferentia from animals sacrificed at 3:00 P.M. and incubated "in vitro" with melatonin (100 pg/ml) increased reaching nocturnal values. In conclusion, it seems that a functional pineal gland, most probably through the synthesis and release of melatonin, is necessary for expression (circadian) and modulation (ultradian) of the rhythmicity in the maximal acetylcholine-induced contraction in the prostatic portion of the rat vas deferens.
Carneiro
R C
RC
Department of Pharmacology, University of São Paulo, Brazil.
Cipolla-Neto
J
J
Markus
R P
RP
D000109:Acetylcholine / Q000494:pharmacology / Q000502:physiology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D004558:Electric Stimulation; D005727:Ganglia, Spinal / Q000502:physiology; D015171:Ganglionectomy; D005730:Ganglionic Blockers / Q000494:pharmacology; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D009638:Norepinephrine / Q000494:pharmacology; D010870:Pineal Gland / Q000187:drug effects / Q000502:physiology*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000502:physiology*; D014649:Vas Deferens / Q000187:drug effects / Q000502:physiology*
D005730:Ganglionic Blockers; D006584:Hexamethonium Compounds; D011978:Receptors, Nicotinic; D018738:Hexamethonium; D000255:Adenosine Triphosphate; D000109:Acetylcholine; D011188:Potassium; D009638:Norepinephrine