Reprogramming of the transcriptional machinery in Xenopus oocytes by injection of mouse poly(A)+ RNA.
We have devised an assay for species-specific transcription factors for the mouse rRNA gene by exploiting the ability of Xenopus oocytes to transcribe injected DNA and translate mRNAs. When mouse rRNA genes are microinjected into Xenopus oocytes, they are not transcribed. We show here that transcription of mouse rRNA genes is supported when mouse mRNAs are injected before the transcription template is injected, indicating that the necessary transcription factors are translated in the oocyte and are available to transcribe an appropriate template. The use of this assay in cloning genes for transcription factors is discussed.
Department of Molecular and Cellular Biology, University of California, Berkeley 94720.
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Dunaway
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D000818:Animals; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D005821:Genetic Techniques; D051379:Mice; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000008:administration & dosage / Q000235:genetics*; D014176:Protein Biosynthesis; D012313:RNA / Q000008:administration & dosage / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014981:Xenopus
D004275:DNA, Ribosomal; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA