Discrimination of Corynebacterium glutamicum, Brevibacterium flavum and Brevibacterium lactofermentum by restriction pattern analysis of DNA adjacent to the hom gene.
Different strains of Corynebacterium glutamicum, Brevibacterium flavum, and Brevibacterium lactofermentum were analysed for restriction fragment length polymorphism using the homoserine dehydrogenase gene (hom) as a probe. The hybridization patterns obtained PvuII- or Asp700-restriction of chromosomal DNA were specific and distinguishable for each of the three species and identical for the different strains of each species. Thus, the method employed allows rapid distinction of Corynebacterium glutamicum, Brevibacterium flavum, and Brevibacterium lactofermentum. The former species could also be discriminated from the latter two by its resistance to 0.5 g/l of the methionine analog ethionine.
Institut für Biotechnologie 1 des Forschungszentrums Jülich, GmbH, F.R.G.
B J
BJ
Eikmanns
M
M
Kircher
D J
DJ
Reinscheid
D015139:Blotting, Southern; D001951:Brevibacterium / Q000145:classification* / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D003352:Corynebacterium / Q000145:classification* / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005001:Ethionine / Q000494:pharmacology; D006715:Homoserine Dehydrogenase / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D015342:DNA Probes; D004269:DNA, Bacterial; D006715:Homoserine Dehydrogenase; D005001:Ethionine