Haemolytic, oedema and haemorrhage inducing activities of tentacular extract of the blubber jellyfish (Catostylus mosaicus).
1. An extract prepared from the tentacle of the jellyfish (CE), Catostylus mosaicus exhibited haemolytic, oedema and haemorrhage-inducing activities. 2. Acetone treatment of the tentacle extract produced an acetone soluble extract (AE) which showed an increase in specific haemolytic and haemorrhagic activities by 25- and 120-fold respectively; the minimum oedema dose was reduced by 30-fold. 3. The AE caused a rapid onset of oedema in the mouse foot pad. The effect was long-lasting, reaching a maximum in about 30 min after injection and sustained up to 4 hr. 4. Fractionation of the AE on Q-Sepharose gave 4 bound fractions which induced oedema and haemorrhage; however only 3 of the fractions exhibited haemolytic activity.
Azila
N
N
Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur.
Siao
F K
FK
Othman
I
I
D000096:Acetone; D000818:Animals; D002852:Chromatography, Ion Exchange; D003064:Cnidarian Venoms / Q000302:isolation & purification / Q000633:toxicity*; D004487:Edema / Q000139:chemically induced*; D006461:Hemolysis; D006470:Hemorrhage / Q000139:chemically induced*; D007616:Kaolin / Q000633:toxicity; D007700:Kinetics; D051379:Mice; D011205:Povidone / Q000633:toxicity; D011817:Rabbits; D012995:Solubility
D003064:Cnidarian Venoms; D000096:Acetone; D007616:Kaolin; D011205:Povidone