Luminography--a new, highly sensitive visualization method for electrophoresis.
A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light-emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X-ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.
Abteilung Allgemeine Paediatrie, Klinikum der Christian-Albrechts-Universität, Kiel, Germany.
R
R
Schneppenheim
U
U
Budde
N
N
Dahlmann
P
P
Rautenberg
D000818:Animals; D000910:Antibodies, Heterophile / Q000032:analysis; D001345:Autoradiography; D015153:Blotting, Western / Q000379:methods*; D002863:Chromogenic Compounds; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005903:Gliadin / Q000276:immunology; D015483:HIV Antibodies / Q000032:analysis; D006735:Horseradish Peroxidase / Q000032:analysis; D006801:Humans; D006861:Hydrogen Peroxide; D007074:Immunoglobulin G / Q000032:analysis; D007525:Isoelectric Focusing / Q000379:methods*; D008163:Luminescent Measurements; D008165:Luminol; D051379:Mice; D011503:Transglutaminases / Q000032:analysis; D014841:von Willebrand Factor / Q000032:analysis
D000910:Antibodies, Heterophile; D002863:Chromogenic Compounds; D015483:HIV Antibodies; D007074:Immunoglobulin G; D014841:von Willebrand Factor; D008165:Luminol; D005903:Gliadin; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D011503:Transglutaminases