[The determination of the antibodies and the somatic O-antigen of Salmonella group B by using the complement-bound lysis of liposomes].
A simple and sensitive method for the determination of group B Salmonella O-antigen and specific antibodies to group B Salmonella by means of the complement-dependent lysis of liposomes sensitized with S. typhimurium lipopolysaccharide (LPS) is proposed. The factors affecting the sensitivity of the method during the determination of antibodies and free antigen have been studied. The method permits the determination of soluble LPS antigen in concentrations of 0.5-200 micrograms/ml.
Skopinskaia
S N
SN
Iarkov
S P
SP
Rubtsov
I V
IV
D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D015941:Complement Hemolytic Activity Assay / Q000379:methods*; D004306:Dose-Response Relationship, Immunologic; D006386:Hemagglutination Tests; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D008081:Liposomes; D019081:O Antigens; D012480:Salmonella Infections / Q000175:diagnosis; D012486:Salmonella typhimurium / Q000276:immunology*; D012995:Solubility
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D008081:Liposomes; D019081:O Antigens
Opredelenie antitel i somaticheskogo O-antigena sal'monell gruppy B s pomoshch'iu komplementzavisimogo lizisa liposom.