Analysis of proteins and lipopolysaccharides from Chinese isolates of Coxiella burnetii with monoclonal antibodies.
Four Coxiella burnetii isolates in China and two reference strains were compared by SDS-PAGE and immunoblotting. The SDS-PAGE profiles of whole cells and LPS of Chinese isolates Qiyi, Xinqiao, and YS-8 were found closely related to Henzerling strain, and different from the Grita strain. In immunoblot assay of LPS and proteinase K-digested whole rickettsiae minor differences were seen in polysaccharide structure among the Chinese isolates by phase I monoclonal antibody. The present results suggest that the strains reported here may be divided into three groups according to the polysaccharide structure: Xinqiao and Henzerling strains (1), YS-8 and Grita (2), and Qiyi (3).
Wen
B H
BH
Department of Microbiology, Third Military Medical College, People's Republic of China.
Yu
S R
SR
Yu
G Q
GQ
Li
Q J
QJ
Zhang
X
X
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000302:isolation & purification; D002681:China; D016997:Coxiella burnetii / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D008070:Lipopolysaccharides