Modulation of histamine release by rat peritoneal mast cells in a superfusion set-up.
A new method is presented for studying mediator release in isolated peritoneal cells of the rat. Using a superfusion technique, cells can be maintained in a continuously cleared medium. Cells were stimulated with antigen, compound 48/80 or A23187 to characterize this preparation. In addition, inhibitory effects of theophylline, isoprenaline and cromoglycate on compound 48/80-induced histamine release were studied. We conclude that superfusion is a valid alternative to study mediator release from isolated peritoneal cells.
Department of Pharmacotherapy, Faculty of Pharmacy, University of Utrecht, The Netherlands.
M J
MJ
Post
C
C
Moekoet
J
J
Wemer
H H
HH
van Rooij
A J
AJ
Porsius
D000818:Animals; D000941:Antigens / Q000276:immunology; D000001:Calcimycin / Q000494:pharmacology; D004205:Cromolyn Sodium / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D013806:Theophylline / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000941:Antigens; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D013806:Theophylline; D007545:Isoproterenol; D004205:Cromolyn Sodium