Molecular cloning of a human immunoglobulin heavy chain variable (VH) region with anti-myelin-associated glycoprotein activity.
A cDNA clone that encodes the heavy chain variable region (VH) of an IgM M-protein with anti-myelin-associated glycoprotein (MAG) activity secreted by chronic lymphocytic leukemia cells (B-C11) from a patient with peripheral neuropathy was cloned and sequenced. The JH region was identical to the germline JH4 sequence except for deletion of a thymidine residue at the site of D-JH recombination, and the D region showed greatest homology to DM2. Sequence analysis of the VH region revealed greatest homology to VH26, a member of the VH3 gene family, but homology was only 83.7% over 326 bases, suggesting that it was derived from as yet an unidentified member of the VH3 gene family.
Department of Neurology, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
R
R
Desai
L
L
Spatz
T
T
Matsuda
A A
AA
Ilyas
J E
JE
Berman
F W
FW
Alt
E A
EA
Kabat
N
N
Latov
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D006825:Hybridomas / Q000032:analysis; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000276:immunology*; D018992:Myelin-Associated Glycoprotein; D009693:Nucleic Acid Hybridization; D012313:RNA
D000906:Antibodies; D007143:Immunoglobulin Heavy Chains; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D012313:RNA; D004247:DNA