Polymerase chain reaction cloning of L-type calcium channel sequences from the heart and the brain.
The sequences of the highly conserved S4 regions of voltage-sensitive ion channels were used to design oligonucleotide primers for the polymerase chain reaction. Specific fragments of the cDNA encoding L-type calcium channels from the heart, brain, and skeletal muscle were amplified and cloned. The nucleotide sequences of the cardiac and brain calcium channels obtained are identical over this region, and share 78% homology with the skeletal muscle calcium channel. Comparison of the predicted amino acid sequences of our clones with those of other calcium channels reveals unexpected patterns of conservation which suggest alternative exon use.
Huang
P
P
Department of Medicine, Massachusetts General Hospital, Boston 02114.
Temizer
D
D
Quertermous
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid
D015220:Calcium Channels; D012313:RNA