Replacement of P1 Leu18 by Glu18 in the reactive site of turkey ovomucoid third domain converts it into a strong inhibitor of Glu-specific Streptomyces griseus proteinase (GluSGP).
Turkey ovomucoid third domain with P1 Leu18 at its reactive site is an excellent inhibitor of chymotrypsin and elastase and of many other serine proteinases with related specificities. Semisynthetic replacement of P1 Leu18 by Lys18 causes the expected change into a trypsin inhibitor. Strikingly, semisynthetic replacement P1 Leu18 to Glu18 changes turkey ovomucoid third domain into a powerful inhibitor of Glu-specific Streptomyces griseus proteinase, GluSGP. Of the 131 natural avian ovomucoid third domains we have sequenced none have P1 Glu18, but several avian ovomucoid first domains have P1 Glu24. They are weak to moderate inhibitors of GluSGP.
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907.
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Komiyama
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Bigler
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Yoshida
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Noda
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Laskowski
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D005971:Glutamates; D018698:Glutamic Acid; D007700:Kinetics; D007930:Leucine; D008969:Molecular Sequence Data; D010059:Ovomucin / Q000235:genetics* / Q000494:pharmacology; D012697:Serine Endopeptidases; D015842:Serine Proteinase Inhibitors; D013305:Streptomyces griseus / Q000201:enzymology*; D013379:Substrate Specificity; D014422:Turkeys
D005971:Glutamates; D015842:Serine Proteinase Inhibitors; D010059:Ovomucin; D018698:Glutamic Acid; D012697:Serine Endopeptidases; C069136:glutamyl endopeptidase II; D007930:Leucine