Comparison of two phosphoproteins in chicken bone and their similarities to the mammalian bone proteins, osteopontin and bone sialoprotein II.
Two phosphorylated proteins of approximately 66 kDa and approximately 60 kDa mass with different DEAE-Sephacel elution patterns were isolated from chicken bone and were shown to be genetically distinct by both biochemical and immunological analysis. A tryptic peptide from the 60 kDa protein was identified that was similar to a sequence of the rat bone sialoprotein II. Both proteins showed RGD inhibited cell-attachment with the MG-63 osteosarcoma cell, and the approximately 66 kDa phosphoprotein appeared to promote cell adhesion better than human vitronectin. The two phosphoproteins appear to share functional and biochemical characteristics and to be homologous to the mammalian bone phosphoproteins, osteopontin and bone sialoprotein II.
Department of Orthopedic Surgery, Harvard Medical School, Boston, MA.
Y
Y
Gotoh
M D
MD
Pierschbacher
J J
JJ
Grzesiak
L
L
Gerstenfeld
M J
MJ
Glimcher
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D002448:Cell Adhesion; D002460:Cell Line; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins; D007118:Immunoassay; D058574:Integrin-Binding Sialoprotein; D008969:Molecular Sequence Data; D008970:Molecular Weight; D053495:Osteopontin; D010750:Phosphoproteins / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D012795:Sialoglycoproteins / Q000235:genetics*; D019096:Vitronectin
D000596:Amino Acids; D006023:Glycoproteins; C546871:IBSP protein, human; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D010750:Phosphoproteins; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D019096:Vitronectin; D053495:Osteopontin