An internal cysteine plays a role in the maintenance of the latency of human fibroblast collagenase.
The cDNA that encodes the proenzyme form of human fibroblast collagenase has been expressed in Escherichia coli. It has been shown by a number of criteria to be functionally identical with the enzyme isolated from human sources. Mutations of each of three cysteine residues found in procollagenase were constructed by site-directed mutagenesis of the cDNA. The relative activities of these mutants were compared to the wild-type enzyme. All of the mutants retained proteolytic activity, but not necessarily on collagen. Mutations that interfere with the formation of the sulfhydryl bridge in the carboxy-terminal domain in some cases increased and in other cases decreased the rate of casein cleavage. On the basis of extensive autolysis within E. coli of a mutant with a replacement of cysteine-73, the procollagenase molecule produced appeared to be either spontaneously active or perhaps more susceptible to autolytic activation, despite the continued presence of the propeptide. Experiments designed to capture the active forms of the mutant by use of the irreversible inhibitor alpha 2-macroglobulin showed that some degree of latency still persisted in the autolytic mutant. These findings suggest that the cysteine at position 73 is important for maintaining the proenzyme in an inactive state but that the maintenance of latency in MMPs may be a complex process, involving a number of interactions between the propeptide domain and the remainder of the collagenase molecule.
Department of Biochemistry, University of Alabama, Birmingham 35294.
L J
LJ
Windsor
H
H
Birkedal-Hansen
B
B
Birkedal-Hansen
J A
JA
Engler
D001483:Base Sequence; D017364:Collagenases; D003545:Cysteine / Q000235:genetics*; D004247:DNA / Q000096:biosynthesis; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000235:genetics; D003012:Microbial Collagenase / Q000096:biosynthesis / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D000511:alpha-Macroglobulins / Q000494:pharmacology
D004792:Enzyme Precursors; D011994:Recombinant Proteins; D000511:alpha-Macroglobulins; D004247:DNA; D017364:Collagenases; D008666:Metalloendopeptidases; C022440:procollagenase; D003012:Microbial Collagenase; D003545:Cysteine