The effect of chronic fatty acid treatment on lipolysis in 3T3-L1 adipocytes.
Various saturated and unsaturated fatty acids were included in the culture medium to test their effects on lipolysis in 3T3-L1 adipocytes. Following prolonged incubation, only oleate was found to exert enhancing effect on basal and isoproterenol-stimulated lipolysis. The effect of oleate was concentration-dependent and was accompanied with increased intracellular cAMP content. Furthermore, the lipolytic response induced by isobutyl-methylxanthine, forskolin or dibutyryl cAMP was also increased in adipocytes treated with oleate. Thus, it appears that in addition to an increased cAMP accumulation, a step distal to cAMP production in the cells may be involved in inducing enhanced lipolysis in 3T3-L1 adipocytes by prolonged exposure to oleate.
Fong
J C
JC
Institute of Biochemistry, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000378:metabolism*; D066298:In Vitro Techniques; D008053:Lipid Mobilization / Q000187:drug effects; D051379:Mice; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*
D005227:Fatty Acids; D009829:Oleic Acids; D005576:Colforsin; D019301:Oleic Acid; D003994:Bucladesine; D015056:1-Methyl-3-isobutylxanthine