Immunocytochemical analysis of early focal cellular infiltrates in experimental oral contact hypersensitivity.
The cellular infiltrates of early contact hypersensitivity reactions to 2,4-dinitrofluorobenzene (DNFB) in rat oral mucosa were phenotypically characterized using serial frozen tissue sections and monoclonal antibodies. CD2+CD45RB- lymphocytes and ED1+RT1B/D+ monocytes/macrophages appeared in focal collections at the epithelium-connective tissue interface 2-6 h after challenge with DNFB. These foci also contained CD4+, CD5+, CD8+, TCR alpha beta+ cells. CD45RB+ "naive" T cells were difficult to detect at 2-6 h but appeared in significant numbers at 24 h post-challenge. At this stage, the number of all the other phenotypes also was increased. CD2+ cells were approximately twice as many as CD5+ or TCR alpha beta+ respectively, indicating that TCR gamma delta+ lymphocytes might be involved. An additional observation was the presence of increased numbers of CD2+ lymphocytes in the oral mucosa of sensitized but not challenged animals. Our findings indicate that the oral mucosa of skin DNFB pre-sensitized animals may be "contact hypersensitivity conditioned" by migration-prone memory T cells, and that these cells may rapidly interact with locally resident epithelial Langerhans' cells following antigen restimulation, creating the very initial antigen-specific part of experimental oral contact hypersensitivity.
Department of Oral Pathology, School of Dentistry, Lund University, Malmö, Sweden.
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Warfvinge
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Larsson
D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D004139:Dinitrofluorobenzene; D004195:Disease Models, Animal; D004342:Drug Hypersensitivity / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D007958:Leukocyte Count / Q000187:drug effects; D008297:Male; D009061:Mouth Mucosa / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013280:Stomatitis / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D013997:Time Factors
D015703:Antigens, CD; D004139:Dinitrofluorobenzene