Cloning and sequencing of protochlorophyllide reductase.
Putative protochlorophyllide reductase cDNA clones (252 and 113) were isolated from an etiolated-oat (Avena sativa) cDNA library. These were used to indirectly characterize a further clone, p127, isolated from a lambda-phage gt11 cDNA library. The latter (1.15 kb in length) was sequenced, and the derived amino acid sequence was shown to be remarkably similar to that derived from chemical analysis of a CNBr-cleavage fragment of the purified reductase, p127 codes for more than 95% of the reductase protein.
Department of Biochemistry, School of Medical Sciences, University of Bristol, U.K.
P M
PM
Darrah
S A
SA
Kay
G R
GR
Teakle
W T
WT
Griffiths
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D002523:Edible Grain / Q000235:genetics*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000235:genetics*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic
D012313:RNA; D004247:DNA; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C023886:protochlorophyllide reductase