Effects of dexamethasone on human synovial fibroblast-like cells, from osteoarthritic joints, in culture.
The effect of Dexamethasone (DEX) on cell division and macromolecular synthesis was investigated in a line (McCoy cells, A 9) of synovial fibroblast-like cells derived from human osteoarthritic joints. DEX markedly reduced the proliferation of McCoy cells in a time and dose-dependent manner. The maximal inhibition (45%) was found at 500 nM DEX 24 h after incubation and was accompanied by the appearance of giant macrophage-like cells. After DEX treatment cells showed increased content of DNA, proteins and RNA together with the reduction of [3H]-thymidine incorporation into the TCA-precipitable fraction.
Institute of Biological Chemistry, University of Palermo, Italy.
R
R
Vento
M V
MV
Torregrossa
M
M
Guiliano
G
G
Grecomoro
F
F
Piccione
D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D010003:Osteoarthritis / Q000473:pathology*; D012313:RNA / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
D012313:RNA; D003907:Dexamethasone; D004247:DNA; D006854:Hydrocortisone