Detection of RNA on northern blots by negative staining with aurintricarboxylic acid.
Aurintricarboxylic acid (ATA) is a well-known inhibitor of RNA and DNA modifying enzymes and was suggested as a potent RNase inhibitor for preparation of RNA (Hallick et al., 1977, Nucleic Acids Res. 4, 3055-3064). We show that ATA is a very useful stain for detecting RNA on Northern blots and slot blots although it did not fully protect purified RNA in concentrated solution against RNase A.
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
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Oberbäumer
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Speth
D000818:Animals; D001312:Aurintricarboxylic Acid; D015152:Blotting, Northern / Q000379:methods; D002460:Cell Line; D003509:Cyclohexanecarboxylic Acids; D004587:Electrophoresis, Agar Gel; D015336:Molecular Probe Techniques; D012313:RNA / Q000032:analysis*; D013194:Staining and Labeling
D003509:Cyclohexanecarboxylic Acids; D001312:Aurintricarboxylic Acid; D012313:RNA