The inhibitory chloride channel of the lobster Panulirus penicillatus neuromuscular junction.
1. Single channel activity was recorded from muscle membranes of the lobster Panulirus penicillatus using the patch-clamp technique. 2. Cell-attached, outside-out and inside-out patches were prepared from the deep abdominal extensor muscle. 3. Low amplitude single channel currents were observed in most patches, and were identified as being chloride-currents. 4. The chloride channel was active spontaneously, and tended to desensitize when outside-out patches were exposed to a small jet of glutamate. 5. Amplitude histograms of single channel currents presented a well defined peak of 8 pA at a membrane potential of -160 mV, while open and closed time histograms were fit to single exponential functions with tau open of 3.27 msec and tau closed of 31.58 msec.
Gilat
E
E
Otto Loewi Center for Cellular and Molecular Neurobiology, Hebrew University of Jerusalem, Israel.
Jaslove
S W
SW
D000818:Animals; D018118:Chloride Channels; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D008565:Membrane Proteins / Q000502:physiology*; D008121:Nephropidae / Q000502:physiology*; D009469:Neuromuscular Junction / Q000502:physiology*; D015221:Potassium Channels / Q000502:physiology
D018118:Chloride Channels; D007473:Ion Channels; D008565:Membrane Proteins; D015221:Potassium Channels