Neutralizing monoclonal antibody against enterovirus-70 reacts with viral proteins 1C and 1D.
A library of murine monoclonal antibodies against the prototype Enterovirus-70 (EV-70) strain, J670/71, was made for the purpose of studying the immunologically reactive determinants of the virus. Each of the monoclonal antibodies reacted with several other strains of Enterovirus-70 when tested by immunofluorescence. However, none of these monoclonal antibodies reacted with any other picornavirus tested. It was found that all of the monoclonal antibodies precipitated EV-70 viral proteins 1C and 1D in radio-immunoprecipitation assays. However, only one of these monoclonal antibodies, an IgG3 kappa, was capable of neutralizing the virus.
National Laboratory for Immunology, Laboratory Center for Disease Control, Ottawa, Ontario, Canada.
J A
JA
Wiley
B R
BR
Brodeur
K D
KD
Dimock
S A
SA
Sattar
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D002460:Cell Line; D004770:Enterovirus / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D015531:Radioimmunoprecipitation Assay
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes