Alterations of high-energy phosphate, serum energy substrate and their metabolites after partial hepatectomy in rats.
Partial hepatectomy with resection of the median and left lateral lobes was performed on our experimental rat group. Another group undergoing a sham operation only was compared. The rats from both groups were allowed to eat freely after their operation. Rats were sacrificed at 6, 24, 48, and 72 hours post-operatively. Remnant liver weight, deoxyribonucleic acid (DNA) synthetic rate, DNA content, and mitotic index were chosen for monitoring regeneration. Adenosine triphosphate (ATP) and creatine phosphate (CP) in liver tissue, blood glucose, serum free fatty acid (FFA), total ketone bodies, and ketone body ratio (KBR) were measured. The results showed that: (1) the high-energy phosphate (ATP and CP) in liver tissue decreased sharply in the early phase after partial hepatectomy and recovered gradually; (2) the decrease in creatine phosphate was more marked than ATP; (3) the blood glucose level decreased, while the serum metabolites of FFA markedly increased in the early posthepatectomized phase; and (4) the KBR was markedly depressed in this early postoperative period.
Department of Surgery, National Taiwan University Hospital, Taipei, R.O.C.
H S
HS
Lai
W J
WJ
Chen
K M
KM
Chen
D000255:Adenosine Triphosphate / Q000097:blood*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004247:DNA / Q000032:analysis; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006498:Hepatectomy; D007657:Ketone Bodies / Q000097:blood; D008099:Liver / Q000473:pathology; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D009929:Organ Size; D010725:Phosphocreatine / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007657:Ketone Bodies; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D004247:DNA