[Exclusion chromatography for the separation of cryoprotective agents from freeze-preserved blood cells].
A method for separating low molecular cryoprotectiva from freeze-conserved erythrocyte- and thrombocyte-concentrates by exclusion chromatography has been described. A new vesicular packing material has been used. Only 25 to 30 minutes are necessary in order to separate glycerol respectively dimethylsulphoxide (DMSO) completely from the cells. 86.5% of the erythrocytes and 75.4% of the thrombocytes were recovered after the separation process.
Institut für Transfusiologie und Transplantologie, Humboldt-Universität, Berlin.
L
L
Mavrina
S
S
König
A
A
Klein
H
H
Woehlecke
E
E
Richter
G
G
Matthes
R
R
Ehwald
D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000302:isolation & purification*; D004121:Dimethyl Sulfoxide / Q000302:isolation & purification; D004912:Erythrocytes / Q000166:cytology*; D005990:Glycerol / Q000302:isolation & purification; D006801:Humans
D003451:Cryoprotective Agents; D005990:Glycerol; D004121:Dimethyl Sulfoxide
Ausschlusschromatographie zur Trennung kryoprotektiver Substanzen von gefrierkonservierten Blutzellen.