Facs analysis of myeloid differentiation stages in epiphyseal bone marrow, adjacent to joints affected with rheumatoid arthritis.
To analyze the differentiation stages of myeloids statistically, we adopted a two-color FACS system and used appropriate monoclonal antibodies belonging to CD15, CD16 and CD11b. By using HL60 treated with DMSO or human bone marrow MNCs from patients with rheumatoid arthritis, it was proved that with this system, myeloids could be clearly separated according to differentiation stages. Furthermore, the number of myeloids at certain stages of differentiation in the epiphyseal bone marrow of patients with RA or OA was measured. Nine of 15 samples from RA patients showed immature and relatively mature myeloids, while none of the 8 OA samples did. When the proportions of myeloids in epiphyseal bone marrow MNCs were compared with the clinical features, disease subsets in RA and the degree of synovitis, seemed to be important factors for abnormal myelopoiesis.
Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
H
H
Owaki
K
K
Yukawa
T
T
Ochi
Y
Y
Shimaoka
K
K
Ono
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000473:pathology* / Q000503:physiopathology; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology* / Q000502:physiology; D018845:CD11 Antigens; D002454:Cell Differentiation / Q000502:physiology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006132:Growth Plate / Q000473:pathology* / Q000502:physiology; D006801:Humans; D007596:Joints / Q000473:pathology* / Q000502:physiology; D015470:Leukemia, Myeloid, Acute / Q000473:pathology / Q000503:physiopathology; D016256:Lewis X Antigen; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000473:pathology / Q000503:physiopathology; D011961:Receptors, Fc / Q000276:immunology; D017452:Receptors, IgG; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018845:CD11 Antigens; D016256:Lewis X Antigen; D011961:Receptors, Fc; D017452:Receptors, IgG; D004121:Dimethyl Sulfoxide