Detection of a novel fimbrial structure on the surface of Salmonella enteritidis by using a monoclonal antibody.
A fimbrialike structure expressed on the surface of Salmonella enteritidis was identified by using a monoclonal antibody (69/25) produced against intact S. enteritidis cells. Fimbriae were less than 5 nm in diameter and carried a protein consisting of subunits with a molecular weight of 14,300. No hemagglutinating activity associated with the fimbriae was detected. An epitope on the fimbrial antigen identified by monoclonal antibody 69/25 was expressed by all 58 S. enteritidis strains, 12 of 36 S. dublin strains, and a single strain of S. moscow examined. None of 169 other isolates tested from 17 salmonella serogroups expressed this epitope.
Central Veterinary Laboratory, Ministry of Agriculture, Fisheries and Food, Weybridge, Surrey, United Kingdom.
C J
CJ
Thorns
M G
MG
Sojka
D
D
Chasey
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D000939:Epitopes; D010861:Fimbriae, Bacterial / Q000276:immunology / Q000648:ultrastructure*; D006386:Hemagglutination Tests; D008854:Microscopy, Electron; D012477:Salmonella enteritidis / Q000145:classification / Q000276:immunology / Q000648:ultrastructure*; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D000939:Epitopes