The inhibitory chloride channel of the lobster Panulirus penicillatus neuromuscular junction.
1. Single channel activity was recorded from muscle membranes of the lobster Panulirus penicillatus using the patch-clamp technique. 2. Cell-attached, outside-out and inside-out patches were prepared from the deep abdominal extensor muscle. 3. Low amplitude single channel currents were observed in most patches, and were identified as being chloride-currents. 4. The chloride channel was active spontaneously, and tended to desensitize when outside-out patches were exposed to a small jet of glutamate. 5. Amplitude histograms of single channel currents presented a well defined peak of 8 pA at a membrane potential of -160 mV, while open and closed time histograms were fit to single exponential functions with tau open of 3.27 msec and tau closed of 31.58 msec.
Otto Loewi Center for Cellular and Molecular Neurobiology, Hebrew University of Jerusalem, Israel.
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Gilat
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Jaslove
D000818:Animals; D018118:Chloride Channels; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D008565:Membrane Proteins / Q000502:physiology*; D008121:Nephropidae / Q000502:physiology*; D009469:Neuromuscular Junction / Q000502:physiology*; D015221:Potassium Channels / Q000502:physiology
D018118:Chloride Channels; D007473:Ion Channels; D008565:Membrane Proteins; D015221:Potassium Channels