Mammalian regulatory peptide immunoreactivity in the trematode parasite Haplometra cylindracea and the lung of its frog host, Rana temporaria: comparative chromatographic characterisation using reverse-phase high-performance liquid chromatography.
1. Extracts of Haplometra cylindracea and lung tissues of its host, Rana temporaria, were subjected to radioimmunoassay using antisera to nine mammalian regulatory peptides. 2. In these extracts, immunoreactivity was measured to pancreatic polypeptide, substance P, neurokinin A, gastrin-releasing peptide and glucagon. The levels of each peptide varied considerably with some marked differences between those demonstrable in parasite and host (notably pancreatic polypeptide). 3. Reverse-phase HPLC fractionation of extracts revealed general chemical differences between parasite and host peptides, with some peptides present in more than one molecular form.
McKay
D M
DM
Division of Cell and Experimental Biology, School of Biology and Biochemistry, Queen's University of Belfast, Northern Ireland, U.K.
Shaw
C
C
Halton
D W
DW
Johnston
C F
CF
Fairweather
I
I
Buchanan
K D
KD
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019886:Gastrin-Releasing Peptide; D005755:Gastrins / Q000032:analysis; D005934:Glucagon / Q000032:analysis; D008168:Lung / Q000737:chemistry; D008322:Mammals; D015288:Neurokinin A / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D011863:Radioimmunoassay; D011896:Rana temporaria / Q000378:metabolism* / Q000469:parasitology; D013373:Substance P / Q000032:analysis; D014200:Trematoda / Q000032:analysis*
D005755:Gastrins; D009479:Neuropeptides; D010455:Peptides; D013373:Substance P; D019886:Gastrin-Releasing Peptide; D015288:Neurokinin A; D005934:Glucagon