Rapid detection of Pneumocystis carinii in bronchoalveolar lavage samples by using Cellufluor staining.
Cellufluor (Calcofluor white) has been found to be a useful, rapid chemofluorescent stain for detection of Pneumocystis carinii cysts in bronchoalveolar lavage samples. When compared with toluidine blue O and Giemsa stains on 45 specimens (22 positive and 23 negative), the sensitivity and specificity of the Cellufluor stain were 95 and 100%, respectively.
Baselski
V S
VS
Department of Pathology, University of Tennessee, Memphis 38163.
Robison
M K
MK
Pifer
L W
LW
Woods
D R
DR
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D005456:Fluorescent Dyes; D006801:Humans; D009930:Organic Chemicals; D011010:Pneumocystis / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis* / Q000382:microbiology; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D009930:Organic Chemicals; C048787:cellufluor