Administration of D-alanine did not cause increase of D-amino acid oxidase activity in mice.
D-amino acid oxidase (DAAO) activity was not altered in the liver and kidney by oral administration of D-alanine to adult mice. The enzyme was apparently not induced by the enteric microflora either, since the enzyme activity in the liver and kidney of germ-free mice was not different from that of specific-pathogen-free mice. The times of appearance of DAAO activity and of free D-amino acids in the kidney were elucidated using suckling mice. DAAO activity started to increase 7 days after birth, and reached almost the adult level by 28 days. The content of free neutral D-amino acids also increased with age, in a similar fashion. A possible conclusion is that the enzyme activity normally increases during this period, to eliminate the free D-amino acids which have increased with age in the suckling mice. Consequently, the administration of D-alanine had no further effect in increasing enzyme activity.
Laboratory of Biology, Himeji Institute of Technology, Japan.
Y
Y
Nagata
R
R
Yamada
H
H
Nagasaki
R
R
Konno
Y
Y
Yasumura
D000409:Alanine / Q000494:pharmacology*; D000596:Amino Acids / Q000097:blood / Q000378:metabolism / Q000652:urine; D000818:Animals; D003605:D-Amino-Acid Oxidase / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
D000596:Amino Acids; D003605:D-Amino-Acid Oxidase; D000409:Alanine