Contribution of the CD5+ B cell to D-proximal VH family expression early in ontogeny.
In this study, the contribution of the CD5+ B cell to the preferential expression of VH 7183 and Q52 observed early in development was determined. CD5+ and CD5- B cells from BALB/c mice were isolated by fluorescence-activated cell sorter and the expression of particular VH gene families was determined directly by in situ hybridization. The results indicate that CD5+ B cells obtained from both adult and neonatal animals express Q52 at increased levels compared with CD5- B cells. Preferential expression of VH 7183 was observed only in the neonatal CD5- B cell subset. Thus, the increased expression of VH 7183 early in development is caused by the CD5- subset whereas increased Q52 expression is caused by the CD5+ subset. These results indicate that the fetal/neonatal conventional B cell is distinct from conventional adult B cells in terms of Ig gene repertoire expression.
Jeong
H D
HD
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284-7758.
Teale
J M
JM
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000943:Antigens, Differentiation / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000502:physiology*; D018956:CD5 Antigens; D002454:Cell Differentiation; D015342:DNA Probes; D015870:Gene Expression; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010529:Peritoneal Cavity / Q000166:cytology; D013154:Spleen / Q000166:cytology
D000943:Antigens, Differentiation; D018956:CD5 Antigens; D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D008070:Lipopolysaccharides