[Behavior of pericentromere heterochromatin regions under different conditions of chromosome decondensation in isolated nuclei].
In isolated mouse nuclei the chromocenters were shown to be the pericentromeric heterochromatin regions (PCHR). After the decreasing of bivalent ion concentration (0.1 mM Ca2+, 2 mM Mg2+) the main and peripheral parts of the chromatin remained on the contrary as the compact chromatin bodies. The additional ultrasound treatment of isolated nuclei in the presence of 0.1 mM Ca2+ with DNAase II and triton X-100 resulted in the species enriched by the condensed PCHR.
E V
EV
Stefanova
Iu S
IuS
Chentsov
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002503:Centromere / Q000648:ultrastructure; D004706:Endodeoxyribonucleases; D006570:Heterochromatin / Q000648:ultrastructure*; D008099:Liver / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron; D013194:Staining and Labeling
D006570:Heterochromatin; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II
Povedenie raĭonov pritsentromernogo geterokhromatina v razlichnykh usloviiakh dekondensatsii khromatina vydelennykh iader.