Mapping of the immunodominant regions of the NAD-dependent formate dehydrogenase.
A panel of 4 monoclonal antibodies and 7 polyclonal antisera against NAD-dependent formate dehydrogenase from methylotrophic bacterium Pseudomonas sp. 101 has been obtained. The reactivity of the 37 overlapping proteolytic peptides with the monoclonal antibodies and polyclonal antisera has been studied with ELISA test. The data obtained were interpreted residing on the structural model of the formate dehydrogenase at 3 A resolution. The immunodominant regions in the formate dehydrogenase molecule and the epitopes for the monoclonal antibodies were elucidated.
A.N. Bakh Institute of Biochemistry, USSR Acad. Sci., Moscow.
A V
AV
Bogdanova
T V
TV
Cherednikova
T A
TA
Egorov
E G
EG
Harutyunyan
N A
NA
Kurochkina
V S
VS
Lamzin
A P
AP
Savitskiy
I A
IA
Shumilin
V O
VO
Popov
D000445:Aldehyde Oxidoreductases / Q000276:immunology*; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D005560:Formate Dehydrogenases / Q000276:immunology*; D006868:Hydrolysis; D007106:Immune Sera / Q000032:analysis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology; D010449:Peptide Mapping; D011549:Pseudomonas / Q000201:enzymology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007106:Immune Sera; D010446:Peptide Fragments; D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases