Antiidiotypic antibody mimicry of a bluetongue virus neutralizing antigen.
Syngeneic antiidiotypic (Anti-Id) mAb were produced to the Bluetongue virus (BTV) serotype 17 neutralizing mAb. Three anti-Id mAb had the characteristics of an internal image of the Id as demonstrated by 1) the internal image anti-Id mAb were capable of mimicking BTV by blocking the neutralizing activity of the idiotypic neutralizing mAb and 2) the anti-Id mAb bound specifically to the surface of BTV susceptible cells in indirect binding experiments as determined by immunocytochemistry and flow cytometric analysis. The potential application of these internal image anti-Id mAb in this arbovirus system is discussed.
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
F B
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Grieder
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Schultz
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D001667:Binding, Competitive; D015153:Blotting, Western; D001820:Bluetongue virus / Q000276:immunology*; D000939:Epitopes; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D012087:Reoviridae / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D007130:Immunoglobulin Idiotypes