Characterization of cDNA encoding a human sperm membrane protein related to A4 amyloid protein.
A rat testis lambda gt11 cDNA library was screened with a monoclonal antibody raised against a human sperm membrane protein designated YWK-II. A clone was found with a cDNA insert composed of 1837 base pairs that contained an open reading frame coding for 191 amino acid residues. The deduced polypeptide contained a segment with high homology to the transmembrane-cytoplasmic domains of the A4 amyloid protein found in brain plaques of Alzheimer disease patients. A sequence of basic amino acid residues, Arg-Lys-Arg, was found instead of Lys-Lys-Lys at the probable membrane-cytoplasmic junction that may be a unique property of sperm membrane proteins.
Yan
Y C
YC
Shanghai Institute of Cell Biology, Academia Sinica, China.
Bai
Y
Y
Wang
L F
LF
Miao
S Y
SY
Koide
S S
SS
D000595:Amino Acid Sequence; D000682:Amyloid / Q000235:genetics*; D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics*; D000939:Epitopes / Q000032:analysis; D015723:Gene Library; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D011487:Protein Conformation; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism
C489697:APLP1 protein, human; C084104:APLP2 protein, human; D000682:Amyloid; D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D000911:Antibodies, Monoclonal; C498283:Aplp2 protein, rat; D000939:Epitopes; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D004247:DNA