The role of a conserved guanosine residue in the hammerhead-type RNA enzyme.
We have designed a hammerhead-type RNA system which consists of three RNA fragments for normal and modified complexes which contain a non-cleavable substrate with 2'-O-methylcytidine and a guanosine-to-inosine replaced enzyme. Examination of the RNA-cleaving activity and conformational properties of the complexes suggests that the 2-amino group of a conserved guanosine residue in the loop region plays an important role for maintaining both the activity and loop conformation.
Odai
O
O
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Hiroaki
H
H
Sakata
T
T
Tanaka
T
T
Uesugi
S
S
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D006151:Guanosine / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000378:metabolism; D016337:RNA, Catalytic; D012335:RNA, Ribosomal / Q000378:metabolism
D016337:RNA, Catalytic; D012335:RNA, Ribosomal; D006151:Guanosine; D012313:RNA