Bacteria and endotoxin enhance basophil histamine release and potentiation is abolished by carbohydrates.
Histamine release caused by anti-IgE, specific antigens and calcium ionophore A23187 was examined in leukocyte suspensions from healthy individuals and patients allergic to house dust mite and birch pollen. Staphylococcus aureus and LPS from Salmonella typhimurium were found to cause a synergistic enhancement of the release. The potentiation of mediator release by the bacteria and the endotoxin depends on a binding to the basophilocyte, followed by a non-transient event, since the potentiating effect persists after preincubation of the cells with the LPS followed by washout and leaving the cells for 30 min at 37 degrees C before stimulation with anti-IgE. The potentiation was abolished or reduced by galactose (10(-7) and 10(-6) M) and N-acetylglucosamine (10(-6) and 10(-5) M), acting by a binding to the basophil cell membrane, demonstrated by the persistence of effect after preincubation and washout of unbound sugar.
Dept. of Pharmacology, University of Copenhagen, Denmark.
P
P
Clementsen
S
S
Norn
K S
KS
Kristensen
N
N
Bach-Mortensen
C
C
Koch
H
H
Permin
D000328:Adult; D001249:Asthma / Q000276:immunology / Q000382:microbiology; D001491:Basophils / Q000276:immunology; D004357:Drug Synergism; D004731:Endotoxins / Q000494:pharmacology*; D005690:Galactose / Q000494:pharmacology*; D006636:Histamine Release; D006801:Humans; D008875:Middle Aged; D012486:Salmonella typhimurium; D013211:Staphylococcus aureus / Q000502:physiology*
D004731:Endotoxins; D005690:Galactose