Thin-layer chromatographic analysis of myelin lipids, their differential O-deacylation by primary alkylamines and their selective staining by thionine. A limited phylogenetic study.
A silica gel thin-layer chromatographic procedure is described for the study of the myelin lipid patterns in a small phylogenetic series of nerve tissue specimens. It involves the selective staining by the thiazine dye thionine and the interpretations were facilitated by a preceding primary alkylamine O-deacylation step. Glycolipids, including sulfatides, and ethanolamine plasmalogens were the principal characterizing lipids.
Hack
M H
MH
Department of Biology, Delaware State College, Dover 19901.
Helmy
F M
FM
D000215:Acylation; D000818:Animals; D002518:Ceramides / Q000032:analysis; D002855:Chromatography, Thin Layer; D049832:Decapodiformes / Q000032:analysis; D005021:Ethylamines; D005399:Fishes; D006017:Glycolipids / Q000032:analysis; D006168:Guinea Pigs; D007798:Lampreys; D008055:Lipids / Q000032:analysis*; D008744:Methylamines; D009186:Myelin Sheath / Q000032:analysis*; D009337:Necturus; D010640:Phenothiazines; D010802:Phylogeny; D012754:Sharks; D013194:Staining and Labeling
D002518:Ceramides; D005021:Ethylamines; D006017:Glycolipids; D008055:Lipids; D008744:Methylamines; D010640:Phenothiazines; C027451:methylamine; C009469:thionine; C041564:ethylamine