Cross-reactions between Actinobacillus (Haemophilus) pleuropneumoniae strains of serotypes 1 and 9.
Actinobacillus pleuropneumoniae strains of serotypes 1 and 9 were studied for their serological properties by means of agglutination, coagglutination (CoA), indirect hemagglutination (IHA), Co-IHA, ring precipitation (RP), and immunodiffusion (ID) tests. Particulate and soluble antigens of unheated and heat-treated bacterial cells were used in various serological tests. Agglutination, CoA, and RP tests demonstrated common antigens between strains of serotypes 1 and 9. Quantitative estimation of serotype-specific antigenic activity by CoA, RP, and ID tests proved useful in differentiating strains of serotypes 1 and 9. IHA and Co-IHA tests using sheep erythrocytes sensitized with unheated or heat-treated whole-cell saline extract and the ID test using boiled whole-cell saline extract as antigen distinguished the strains of serotypes 1 and 9. In studies of absorption of rabbit antisera with heterologous whole-cell antigens there was no absorption of antibodies in tube agglutination and IHA tests, suggesting that serotype 1 and 9 strains belong to two distinct serogroups. It appears that the cross-reactivity between serotype 1 and 9 strains could be due to common epitopes associated with cell wall antigens.
Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Quebec, Canada.
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Mittal
D000188:Actinobacillus / Q000145:classification / Q000276:immunology*; D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D006190:Haemophilus / Q000145:classification / Q000276:immunology*; D006386:Hemagglutination Tests; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion; D011001:Pleuropneumonia / Q000382:microbiology / Q000662:veterinary; D011233:Precipitin Tests; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
D000942:Antigens, Bacterial; D000939:Epitopes; D007106:Immune Sera