Lectin binding patterns to terminal sugars of rat lung alveolar epithelial cells.
We used post-embedding cytochemical techniques to investigate the lectin binding profiles of rat lung alveolar epithelial cells. Sections from rat lung embedded in the hydrophilic resin Lowicryl K4M were incubated either directly with a lectin-gold complex or with an unlabeled lectin followed by a specific glycoprotein-gold complex. The binding patterns of the five lectins used could be divided into three categories according to their reactivity with alveolar epithelial cells: (a) the Limax flavus lectin and Ricinus communis I lectin bound to both type I and type II cell plasma membranes; (b) the Helix pomatia lectin and Sambucus nigra L. lectin bound to type II but not type I cells; and (c) the Erythrina cristagalli lectin reacted with type I cells but was unreactive with type II cells. The specificity of staining was assessed by control experiments, including pre-absorption of the lectins with various oligosaccharides and enzymatic pre-treatment of sections with highly purified glycosidases to remove specific sugar residues. The results demonstrate that these lectins can be used to distinguish between type I and type II cells and would therefore be useful probes for investigating cell dynamics during lung development and remodeling.
Department of Pathology, University of Vermont, Burlington.
D J
DJ
Taatjes
L A
LA
Barcomb
K O
KO
Leslie
R B
RB
Low
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism / Q000648:ultrastructure; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D006026:Glycoside Hydrolases; D007150:Immunohistochemistry; D037102:Lectins / Q000145:classification / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D009844:Oligosaccharides / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D013194:Staining and Labeling; D014021:Tissue Preservation / Q000379:methods
D037102:Lectins; D009844:Oligosaccharides; D006026:Glycoside Hydrolases