Isolation of an endogenously processed immunodominant viral peptide from the class I H-2Kb molecule.
Using an approach for isolating and characterizing peptide fractions that are intracellularly associated with major histocompatibility complex class I molecules, the major peptide recognized by cytotoxic T cells specific for the vesicular stomatitis virus has been isolated from the H-2Kb molecule of infected cells. This endogenously processed octapeptide is allele-specific as it does not bind to H-2Db molecules, and contains the core sequence of the epitope of the nucleocapsid protein of the vesicular stomatitis virus identified by testing with exogenous synthetic peptides.
Van Bleek
G M
GM
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.
Nathenson
S G
SG
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D002213:Capsid / Q000737:chemistry / Q000276:immunology; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D000939:Epitopes / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D006183:H-2 Antigens / Q000032:analysis*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D014758:Viral Core Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*
D000956:Antigens, Viral; D000939:Epitopes; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D015395:Histocompatibility Antigens Class I; D014758:Viral Core Proteins