Noninfectious doughnut-shaped human immunodeficiency virus type 1 can induce syncytia mediated by fusion of the particles with CD4-positive cells.
A defective doughnut-shaped human immunodeficiency virus type 1 (HIV-1)-producing cell clone (designated as L-2) was isolated from persistently HIV-1-infected MT-4 cells. The syncytium-forming capacity of the cell and virus particle fractions was examined in human CD4-positive T cells. Several cell lines producing infectious HIV-1 particles, such as persistently HIV-1-infected MOLT-4 (MOLT-4/HIV-1) cells, were used as controls. Syncytia were formed within 20 h by the cell fraction of both L-2 and MOLT-4/HIV-1 and the virus particle fraction of L-2, but not MOLT-4/HIV-1. These formations were not affected by 3'-azido-3'-deoxythymidine (ZDV). In contrast, similar syncytium formation was first observed 2 days after the incubation of the virus particle fraction of MOLT-4/LAV-1 and this syncytium formation mediated by the cell fractions of MOLT-4/HIV-1 and L-2 or the virus particle fraction of L-2 differently.
Ohki
K
K
Section of Serology, Hokkaido University, Sapporo, Japan.
Kishi
Y
Y
Nishino
Y
Y
Sumiya
M
M
Kimura
T
T
Goto
T
T
Nakai
M
M
Ikuta
K
K
D000911:Antibodies, Monoclonal / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D002461:Cell Line, Transformed; D003673:Defective Viruses / Q000502:physiology; D015726:Giant Cells / Q000187:drug effects / Q000502:physiology*; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D014407:Tumor Cells, Cultured; D015215:Zidovudine / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D015215:Zidovudine