Cytokeratin expression in rat mesothelial cells in vitro is controlled by the extracellular matrix.
Rat mesothelial cells co-express vimentin and the simple epithelial cytokeratins. While cytokeratins predominate in situ, under most culture conditions vimentin is the major intermediate filament protein of the cells. This loss of cytokeratin production upon culture can be partly prevented by growing mesothelial cells on a basement membrane matrix. However, the basement membrane-promoted persistence of cytokeratin synthesis is not accompanied by expression of cytokeratin G (no. 19), the major acidic cytokeratin of mesothelium in vivo. While cells grown on plastic establish a prominent juxtanuclear assemblage of tonofilaments, those cultured on basement membrane exhibit cytokeratin filaments which are distributed throughout the cytoplasm and attach to neighboring cells at the plasma membrane. This latter pattern resembles that seen in the intact mesothelium. Intermediate filaments are markers of cellular differentiation, but their roles are obscure. The response of cultured mesothelial cells to different growth substrata supports the hypothesis that intermediate filament synthesis is influenced by cellular contact with the extracellular matrix.
Department of Pathology, University of Vermont, College of Medicine, Burlington 05405.
A M
AM
Mackay
R P
RP
Tracy
J E
JE
Craighead
D000818:Animals; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology*; D007382:Intermediate Filaments / Q000502:physiology; D007633:Keratins / Q000378:metabolism*; D008297:Male; D008648:Mesoderm / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007633:Keratins