[Two cases of ulcerative colitis with colon cancer: immunoperoxidase staining using monoclonal antibodies against gastrointestinal tumor and mucin staining].
Surgical specimens from 2 patients with chronic ulcerative colitis accompanied with colon cancer were evaluated by immunoperoxidative staining using monoclonal antibodies A7 (against human colon cancer), S202 (against human gastric cancer), and anti-carcinoembryonic antigen (CEA). The three monoclonal antibodies were reactive with cancerous tissue, anti-CEA antibody and monoclonal antibody S202 reacted with dysplasia tissues, whereas monoclonal antibody A7 did not. Using high-iron diamine technique for mucosubstances (sialomucin and sulfomucin), cancer and dysplasia showed no secretory elements. Surrounding mucosa showed both sialomucin and sulfomucin secretion.
First Department of Surgery, Kyoto Prefectural University of Medicine.
J
J
Okuzumi
T
T
Yokota
K
K
Sawai
S
S
Kondoh
K
K
Kuwata
M
M
Tanaka
T
T
Yamane
T
T
Yamaguchi
Y
Y
Fujita
T
T
Takahashi
D000230:Adenocarcinoma / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D000911:Antibodies, Monoclonal; D003093:Colitis, Ulcerative / Q000150:complications / Q000473:pathology* / Q000503:physiopathology; D003110:Colonic Neoplasms / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D009077:Mucins / Q000378:metabolism; D052116:Sialomucins; D013194:Staining and Labeling; D013274:Stomach Neoplasms / Q000473:pathology*
D000911:Antibodies, Monoclonal; D009077:Mucins; D052116:Sialomucins; C012564:sulfomucin