[Gene diagnosis of familial erythroleukemia at the early stage of leukomogenesis].
Southern blot hybridization was performed with probe v-erbB + A in 4 subjects. Two of them were patients with similar erythroid alterations of bone marrow: one of them was diagnosed as erythroleukemia (EL). The other two were members of a same family (husband and wife). The husband had normal erythroid cellularity of bone marrow but the wife and their 7 living sons and daughters had erythroid hypercellularity. In the second generation of this family 4 hematological patients (1 with AML and 3 with CAA) were found in the past decade. Rearrangement of cerbB and c-erbA genes was found in 3 of the 4 subjects (2 with EL and the wife mentioned above). They had 4 new hybridization bands besides 3 germlines, but the husband had only 3 germlines. The result suggests that using Southern blot hybridization technique with probe v-erbB + A, the rearrangement of c-erbB/c-erbA genes might be a diagnostic indicator of erythroleukemia at early stage of leukomogenesis in familial and sporadic patients. The etiological significance of the rearrangement of c-erbB/c-erbA in leukomogenesis was discussed.
Feng
B Z
BZ
Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin.
Lei
J L
JL
Chu
Y L
YL
D000293:Adolescent; D000328:Adult; D001355:Alpharetrovirus / Q000235:genetics; D002648:Child; D002873:Chromosome Fragility; D015342:DNA Probes; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D018774:Oncogene Proteins v-erbA; D010375:Pedigree; D015682:Retroviridae Proteins, Oncogenic
D015342:DNA Probes; D018774:Oncogene Proteins v-erbA; D015682:Retroviridae Proteins, Oncogenic