Characterization of the murine Hox-2.3 promoter: involvement of the transcription factor USF (MLTF).
The murine homeobox-containing gene Hox-2.3 contains a basal promoter in a 210-bp region upstream of the transcription start site. In vitro studies of DNA-protein interactions in this region, and in a 1.3-kb upstream region which is known to play a role in tissue specific expression in vivo, led to the identification of DNA elements interacting with nuclear proteins from embryocarcinoma cells. Among the factors binding to the basal promoter is the upstream stimulating factor (USF), also known as major late transcription factor (MLTF). A single point mutation in its binding site abolishes binding in vitro and leads to 50% reduction of the transcriptional activity as measured in receptor gene experiments, showing that it is an activator of Hox-2.3 expression.
Zwartkruis
F
F
Hubrecht Laboratory, Netherlands Institute for Developmental Biology Utrecht.
Hoeijmakers
T
T
Deschamps
J
J
Meijlink
F
F
D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000032:analysis; D003851:Deoxyribonucleases / Q000032:analysis; D005090:Exodeoxyribonucleases / Q000037:antagonists & inhibitors; D005801:Genes, Homeobox / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014157:Transcription Factors / Q000502:physiology*; D051720:Upstream Stimulatory Factors
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D051720:Upstream Stimulatory Factors; C497183:Usf1 protein, mouse; D003851:Deoxyribonucleases; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III