Effect of prostaglandin E2 on cyclic AMP levels in limb cells of mouse mutant brachypodism.
Mouse embryo limb cells carrying either the brachypodism (bpH/bpH) mutation or its wild-type (+/+) allele were tested for their ability to accumulate cyclic AMP in response to prostaglandin E2 (PGE2) between Embryonic Days E12 and E14. Mutant cells exhibited a precocious increase in cyclic AMP. In the absence of PGE2 but in the presence of the phosphodiesterase inhibitor 1-methyl-3-isobutylxanthine (MIX), the brachypodism cells accumulated a significantly lower amount of cyclic AMP by Day E14. Limb cells carrying the bpH mutation may provide a useful experimental system to study the PGE2-cyclic AMP-cartilage differentiation interrelationship.
Department of Biological Sciences, University of North Carolina, Wilmington 28403.
T A
TA
Ballard
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Elmer
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000483:Alleles; D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D002454:Cell Differentiation; D000242:Cyclic AMP / Q000378:metabolism*; D015232:Dinoprostone / Q000494:pharmacology*; D006614:Hindlimb / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D009154:Mutation
D000242:Cyclic AMP; D015232:Dinoprostone; D015056:1-Methyl-3-isobutylxanthine