Functional expression of H1-histaminergic receptors in Xenopus laevis oocytes injected with bovine adrenal medullary mRNA.
We prepared poly (A)+ mRNA from bovine adrenal medulla and electrophysiologically examined the expression of H1-histaminergic receptors in Xenopus laevis oocytes. Histamine dose-dependently induced inward currents in oocytes injected with the mRNA, but not in those injected with water or the non-injected oocytes. This response was also induced by H1-agonists and antagonized by H1-antagonists, while both an H2-agonist and an antagonist had no effect. These results clearly demonstrate that H1-histaminergic receptors are functionally expressed in oocytes injected with exogenous mRNA.
Department of Cell Biology, Osaka Bioscience Institute, Japan.
K
K
Sugama
M
M
Yamashita
H
H
Fukui
S
S
Ito
H
H
Wada
D000313:Adrenal Medulla; D000818:Animals; D002417:Cattle; D004594:Electrophysiology; D006632:Histamine / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D008564:Membrane Potentials; D009865:Oocytes / Q000502:physiology*; D012333:RNA, Messenger / Q000494:pharmacology*; D011969:Receptors, Histamine H1 / Q000502:physiology*; D014982:Xenopus laevis
D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D012333:RNA, Messenger; D011969:Receptors, Histamine H1; D006632:Histamine