Characterization of murine monoclonal antibodies to the tat protein from human immunodeficiency virus type 1.
A panel of murine monoclonal antibodies (MAbs) to the human immunodeficiency virus type 1 trans-activator tat protein were characterized. The anti-tat MAbs were mapped to the different domains of the tat protein by Western blot (immunoblot) and Pepscan analyses. One-half of the MAbs tested mapped to the amino-terminal proline-rich region, and one-third of the MAbs tested mapped to the lysine-arginine-rich region of tat. The individual MAbs were tested for inhibition of tat-mediated trans activation, using a cell-based in vitro assay system. MAbs which mapped to the amino-terminal region of the tat protein demonstrated the highest degree of inhibition, whereas MAbs reactive to other portions of the molecule exhibited a less pronounced effect on tat function.
Department of Molecular Genetics, Smith Kline and French Laboratories, King of Prussia, Pennsylvania 19406-0939.
D A
DA
Brake
J
J
Goudsmit
W J
WJ
Krone
P
P
Schammel
N
N
Appleby
R H
RH
Meloen
C
C
Debouck
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D000939:Epitopes / Q000032:analysis; D015696:Gene Products, tat / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology; D015534:Trans-Activators / Q000276:immunology*; D054322:tat Gene Products, Human Immunodeficiency Virus
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015696:Gene Products, tat; D009842:Oligopeptides; D015534:Trans-Activators; D054322:tat Gene Products, Human Immunodeficiency Virus