Immunoreactive insulin-like growth factor II in urine.
Insulin-like growth factor II and insulin-like growth factor binding protein-1 were identified and quantified in the urine of 23 healthy subjects between 17 and 76 years of age. IGF-II was measured after separation by gel chromatography at low pH and compared with IGF-I levels in the same samples, whereas IGF binding protein-1 was measured in dialysed urine. Urinary IGF-II was found at much higher concentrations than IGF-I (mean +/- SEM: 717 +/- 69 vs 110 +/- 5 ng/mmol creatinine). The chromatographic profile indicates that pro-IGF-II may also be present. The concentrations of IGF-II appear to be less variable than the other reported parameters. The mean IGF binding protein-1 concentrations in these urine samples was 414 +/- 83 ng/mmol creatinine. IGFs in the urine are in part bound to binding proteins.
Department of Endocrinology, Karolinska Hospital and Institute, Stockholm, Sweden.
W
W
Zumkeller
K
K
Hall
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D002352:Carrier Proteins / Q000652:urine; D002850:Chromatography, Gel; D003404:Creatinine / Q000652:urine; D005260:Female; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I / Q000652:urine; D007335:Insulin-Like Growth Factor II / Q000652:urine*; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay
D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D003404:Creatinine