Detection of ornithine decarboxylase gene expression in 12-O-tetradecanoylphorbol-13-acetate-treated mouse skin using in situ hybridization.
The localization of ornithine decarboxylase gene expression in mouse skin by tumour promoter, 12-O-tetradecanoylphorbol-13-acetate, was investigated using in situ hybridization. After 4 h of treatment with tumour promoter, the grains representing ornithine decarboxylase mRNA increased remarkably in number in the epidermis, especially in the follicular region. When the cells containing more than 5 grains were counted as positive, the number of positive cells increased by about 52-fold in the follicular epidermis, by about 19-fold in the interfollicular epidermis and by 4-fold in the dermis, as compared with controls. These results indicate that the epidermal cells are mainly responsible for the activation of ornithine decarboxylase by 12-O-tetradecanoylphorbol-13-acetate.
Department of Dermatology, Osaka City University Medical School, Japan.
T
T
Kono
M
M
Fukuda
M
M
Ishii
N
N
Mizuno
H
H
Tahara
H
H
Yoshida
I
I
Matsui-Yuasa
S
S
Otani
T
T
Hamada
D000818:Animals; D001345:Autoradiography; D004247:DNA / Q000378:metabolism; D015342:DNA Probes; D005260:Female; D015870:Gene Expression; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D009955:Ornithine Decarboxylase / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000201:enzymology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D015342:DNA Probes; D012333:RNA, Messenger; D004247:DNA; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate