Mapping of transcripts encoded by the plasmid in Chlamydia trachomatis.
Mapping of the plasmid-encoded RNA of the intracellular parasite, Chlamydia trachomatis revealed that the upstream control elements are different from those of other Gram-negative bacteria. A tetranucleotide, AYAA was found near the -10 position, in 5 out of 8 upstream sequences described so far. The plasmid also has a developmentally regulated promoter. The chlamydial upstream elements do not function as promoters in E. coli and vice versa. An E. coli promoter-like sequence has been found to occur fortuitously upstream from the plasmid-encoded dnaB gene. Such sequences may be evolutionary relics.
Menzies School of Health Research, Darwin, N.T., Australia.
K S
KS
Sriprakash
B J
BJ
Pearce
D001483:Base Sequence; D015152:Blotting, Northern; D002692:Chlamydia trachomatis / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D012313:RNA / Q000235:genetics*
D012313:RNA