Acute phase protein stimulation by alpha 1-antichymotrypsin-cathepsin G complexes. Evidence for the involvement of interleukin-6.
Incubation of alpha 1-antichymotrypsin-cathepsin G complexes with human lung fibroblasts caused a nearly 5-fold increase in synthesis of the cytokine interleukin-6. In turn, the fibroblast-conditioned medium induced significant synthesis of the acute phase proteins haptoglobin, fibrinogen, and alpha 1-antichymotrypsin in human Hep G2 cells, whereas a mixture of interleukin-1 and conditioned medium was considerably less stimulatory. These data indicate that proteinase-proteinase inhibitor complexes formed between plasma serpins and their target enzymes could play major roles in signaling for acute phase protein synthesis in response to injury.
Kurdowska
A
A
Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.
Travis
J
J
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D056649:Cathepsin G; D002403:Cathepsins / Q000494:pharmacology*; D002460:Cell Line; D005340:Fibrinogen / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D006242:Haptoglobins / Q000096:biosynthesis; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000096:biosynthesis*; D007700:Kinetics; D011994:Recombinant Proteins / Q000494:pharmacology; D012697:Serine Endopeptidases; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D000514:alpha 1-Antichymotrypsin / Q000494:pharmacology*
D000209:Acute-Phase Proteins; D006242:Haptoglobins; D007375:Interleukin-1; D015850:Interleukin-6; D011994:Recombinant Proteins; D000514:alpha 1-Antichymotrypsin; D005340:Fibrinogen; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D013755:Tetradecanoylphorbol Acetate