The effect of PDGF on the healing of full thickness wounds in hairless guinea pigs.
1. Platelet-derived growth factor (PDGF), administered daily for 5 days and every other day for 9 additional days or the day of wounding and the following day, caused dose dependent (0.2, 1.0 or 5.0 micrograms PDGF/wound) increased granulation tissue. 2. The two day 5.0 micrograms treatment resulted in a 73% increase and the multi-day treatment resulted in a 52% increase of alkaline phosphatase activity three days after wounding. 3. Multi-day treatment resulted in significant increases in protein synthesis (132%), vimentin content (160%) and excised wound weight (76%) three days after wounding. 4. These results indicate that limited administration of PDGF alters wound healing although multiple applications provoke a more dramatic response.
Hill
E
E
Marion Merrell Dow Inc., Kansas City, MO 64134.
Turner-Beatty
M
M
Grotewiel
M
M
Fosha-Thomas
S
S
Cox
C
C
Turman
C
C
Drees
D
D
Baird
L
L
Maratea
D
D
Tucker
R
R
D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D006097:Granulation Tissue / Q000502:physiology; D006168:Guinea Pigs; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000502:physiology*; D014746:Vimentin / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*
D010982:Platelet-Derived Growth Factor; D014746:Vimentin; D000469:Alkaline Phosphatase