Detection of doubly stained fluorescent specimens using confocal microscopy.
Studies of doubly stained specimens were performed with a confocal scanning microscope. The instrument used provides the possibility of making separate detections of the fluorescent dyes. The optimal choice of excitation wavelengths and optical filters are discussed. The fluorphores that were used are Lucifer Yellow, Texas Red, fluorescein isothiocyanate and tetramethylrhodamine isothiocyanate.
Royal Institute of Technology, Stockholm, Sweden.
K
K
Mossberg
M
M
Ericsson
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D005347:Fibroblasts / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000379:methods*; D009474:Neurons / Q000648:ultrastructure; D012235:Rhodamines; D013116:Spinal Cord / Q000166:cytology; D013194:Staining and Labeling; D013861:Thiocyanates
D005452:Fluoresceins; D005456:Fluorescent Dyes; D012235:Rhodamines; D013861:Thiocyanates; C005358:tetramethylrhodamine; D016650:Fluorescein-5-isothiocyanate