[Construction and expression in Escherichia coli of a gene of hybrid hemagglutinin H1-H3 of influenza virus].
The hybrid gene of influenza virus hemagglutinin (HA) of the H1-subtype, carrying the sequence coding for the fragment of H3-subtype antigenic site B, was constructed. The product of expression of this gene in E. coli was obtained as a fusion protein with beta-galactosidase. The chimeric protein was shown to retain the antigenic properties of HA of H1-subtype and to interact specifically with antibodies against the synthetic peptide corresponding to the B site fragment of HA of the H3-subtype.
Petrenko
V A
VA
Kipriianov
S M
SM
Mizenko
G A
GA
Eroshkin
A M
AM
Sivolobova
G F
GF
Rukavishnikov
M Iu
MIu
Akimenko
Z A
ZA
Boldyrev
A N
AN
Kalashnikov
V V
VV
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005813:Genes, Synthetic; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D000939:Epitopes; D006389:Hemagglutinins, Viral; D011994:Recombinant Proteins
Konstruirovanie i ékspressiia v Escherichia coli gena gibridnogo gemaggliutinina podtipa H1-H3 virusa grippa.