Differential regulation of keratin 8 and 18 messenger RNAs in differentiating F9 cells.
F9 embryonal carcinoma cells (F9EC) can be induced to differentiate in vitro into epithelial cells expressing keratin 8 (K8) and keratin 18 (K18). cDNAs corresponding to K8 and K18 mRNAs were cloned and used to study the change in the abundance of these mRNAs during differentiation of F9 cells into parietal endoderm-like cells by treatment with retinoic acid (RA) or with RA and dibutyryl cAMP (Bt2cAMP). Using an RNase protection assay, it was determined that K8 mRNA was induced slightly before K18 mRNA and that it accumulated to a greater extent than K18 mRNA. Furthermore, differentiation in presence of Bt2cAMP plus RA resulted in an earlier induction of the two mRNAs and a higher level of expression of K8 mRNA. These results indicate that K8 and K18 mRNAs are regulated differently in F9 cells.
Institut du Cancer de Montréal, Canada.
T
T
Ouellet
C
C
Lampron
M
M
Lussier
L
L
Lapointe
A
A
Royal
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation; D002460:Cell Line; D003001:Cloning, Molecular; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D007633:Keratins / Q000235:genetics*; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics*; D015183:Restriction Mapping; D013724:Teratoma; D014407:Tumor Cells, Cultured / Q000166:cytology*
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D007633:Keratins