Identification of a potent synthetic HIV1 immunogen compromising gag-P24 tandem T- and B-cell epitopes.
Recent studies indicate that the gag gene products may play a crucial role in the immune response against HIV infection since clinical progression to AIDS is associated with a reduction in the level of circulating antibodies to gag p24 and antibodies raised against p17 peptide can inhibit HIV1 infection in vitro. Using conventional structure prediction algorithms for T-cell and B-cell epitopes, we have selected and chemically synthesized several gag peptides. In particular, an unconjugated HIV1-p24 peptide containing both B- and T-cell epitopes in tandem plus Freund's adjuvant induced a strong antibody response in both mice and rabbits against p24 and its precursor p55 as judged by immunoblotting. In addition, the peptide presented in the appropriate MHC context was shown to be highly stimulatory for p24 specific murine T-cell clones.
Chong
P
P
Connaught Centre for Biotechnology Research, Connaught Laboratories Ltd., Willowdale, Ont., Canada.
Sia
C
C
Sydor
M
M
Klein
M
M
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D015683:Gene Products, gag / Q000276:immunology*; D015483:HIV Antibodies / Q000096:biosynthesis; D015488:HIV Antigens / Q000276:immunology; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000276:immunology*; D007106:Immune Sera; D015151:Immunoblotting; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000276:immunology; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology*
D000939:Epitopes; D015683:Gene Products, gag; D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D007106:Immune Sera; D011498:Protein Precursors; D014758:Viral Core Proteins; C059767:p55 gag precursor protein, Human immunodeficiency virus 1