Quantitative autoradiographic study on tyrosine hydroxylase mRNA with in situ hybridization and alpha 2 adrenergic receptor binding in the locus coeruleus of the spontaneously hypertensive rat.
alpha-2 Adrenergic (A2) receptors and tyrosine hydroxylase (TH) mRNA in the locus coeruleus (LC) were studied using [125I]iodoclonidine and [35S]TH oligonucleotide probe. Spontaneously hypertensive (SHR) rats contained less TH mRNA at their prehypertensive, but not at the well-established hypertensive stage, than age-matched Wistar-Kyoto rats. Furthermore, there is an up-regulation of A2 receptors in SHR rats which is parallel to their blood pressure elevation. The present data suggest that increased A2 receptors in conjunction with TH mRNA reduction in the LC are associated with initiation, but not maintenance of genetic hypertension.
Department of Anatomy, Indiana University School of Medicine, Terre Haute 47809.
J M
JM
Luque
A
A
Guillamon
B H
BH
Hwang
D000818:Animals; D001345:Autoradiography; D003000:Clonidine; D007457:Iodine Radioisotopes; D008125:Locus Coeruleus / Q000378:metabolism*; D008297:Male; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D015854:Up-Regulation
D007457:Iodine Radioisotopes; D009841:Oligonucleotides; D012333:RNA, Messenger; D011942:Receptors, Adrenergic, alpha; D014446:Tyrosine 3-Monooxygenase; D003000:Clonidine