Patterns of reactivity of Epstein-Barr virus-specific T cells in A-type donor cultures after reactivation with autologous A- or B-type transformants.
Regression endpoints were assessed in cultures from 11 Epstein-Barr (EB) virus A-type seropositive donors and 2 seronegative donors using A- and B-type EB virus preparations. In 9/11 of the seropositive donors, the resulting endpoints using A-type or B-type virus were similar and demonstrated a significant T cell response to both virus types. However, the regression endpoints for 2/11 seropositive donors were reproducibly higher with B-type virus compared with A-type virus, indicating a weak T cell response to the B-type virus compared with that to the A-type virus. Seronegative donor cultures showed no regression. The patterns of reactivity of bulk cultures of EB virus-specific cytotoxic T cells and T cell clones from selected seropositive donors were compared. Four of six donors showed evidence of a cytotoxic T cell response to A- and B-type autologous transformants while cytotoxic T cells from 2/6 donors (corresponding to those identified as lacking regression to B-type virus) lysed autologous targets infected with A-type but not B-type virus. The results show that while most A-type seropositive donors are capable of mounting a T cell response to A- and B-type virus, certain donors apparently lack B-type reactivity.
Queensland Institute of Medical Research, Herston, Brisbane, Australia.
S R
SR
Burrows
I S
IS
Misko
T B
TB
Sculley
C
C
Schmidt
D
D
Gang
D J
DJ
Moss
D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000956:Antigens, Viral; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens; D002469:Cell Separation; D002472:Cell Transformation, Viral / Q000276:immunology*; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D000939:Epitopes; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D012044:Regression Analysis; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology* / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology
D000945:Antigens, Differentiation, T-Lymphocyte; D000956:Antigens, Viral; D015704:CD4 Antigens; D016827:CD8 Antigens; D002860:Chromium Radioisotopes; D000939:Epitopes