Cytohistology, colposcopy and in situ hybridization in cervical preneoplastic lesions: a correlative study.
Formalin-fixed, paraffin-embedded cervical tissues from 106 patients which exhibited a colposcopically atypical transformation zone, were examined for the presence of HPV DNA by in situ hybridization using biotin-labeled HPV 6/11 and 16/18 DNA probes. From the comparative study between histologic, cytologic, colposcopic findings and virologic results it is confirmed that HPV 6/11 DNA is detected more frequently in the less severe lesions, whereas HPV 16/18 DNA is predominantly found in the most severe dysplastic lesions.
Department of Obstetrics and Gynecology, IInd University of Rome, Hospital S. Eugenio, Italy.
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D002584:Cervix Uteri / Q000166:cytology / Q000382:microbiology / Q000473:pathology; D016009:Chi-Square Distribution; D003127:Colposcopy; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D015336:Molecular Probe Techniques; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D002578:Uterine Cervical Dysplasia / Q000382:microbiology / Q000473:pathology*; D014626:Vaginal Smears
D015344:DNA Probes, HPV; D004279:DNA, Viral