PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily.
An antibody to a platelet integral membrane glycoprotein was found to cross-react with the previously identified CD31 myelomonocytic differentiation antigen and with hec7, an endothelial cell protein that is enriched at intercellular junctions. This antibody identified a complementary DNA clone from an endothelial cell library. The 130-kilodalton translated sequence contained six extracellular immunoglobulin (Ig)-like domains and was most similar to the cell adhesion molecule (CAM) subgroup of the Ig superfamily. This is the only known member of the CAM family on platelets. Its cell surface distribution suggests participation in cellular recognition events.
The Blood Center of Southeastern Wisconsin, Milwaukee 53233.
P J
PJ
Newman
M C
MC
Berndt
J
J
Gorski
G C
GC
White
S
S
Lyman
C
C
Paddock
W A
WA
Muller
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004730:Endothelium, Vascular / Q000032:analysis / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005803:Genes, Immunoglobulin; D006801:Humans; D015151:Immunoblotting; D007136:Immunoglobulins; D007163:Immunosorbent Techniques; D008969:Molecular Sequence Data; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D011487:Protein Conformation; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D015815:Cell Adhesion Molecules; D000939:Epitopes; D007136:Immunoglobulins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D010980:Platelet Membrane Glycoproteins; D004247:DNA