Proliferating cells in histiocytic necrotizing lymphadenitis.
The phenotypes of proliferating cells in histiocytic necrotizing lymphadenitis (HNL) were examined. The affected areas consisted mainly of CD 8-positive (suppressor/cytotoxic T-cells) and CD 4-positive (helper/inducer T-cells) in association with some CD 15-positive cells (monocytes). A marker of proliferating cells (Ki-67) and monoclonal antibodies for determining the phenotypes of cells (CD 4, CD 8, CD 15) in the affected areas were applied using a double-staining method. Ki-67-positive proliferating cells were mainly CD 8-positive. A few CD 4-positive cells and rare CD 15-positive cells were also Ki-67-positive. The percentage of CD 8-positive cells increased gradually over time and the ratio of CD 8-positive to proliferating cells did not decrease throughout the observation period of 6 weeks. These results suggest that the proliferation of CD 8-positive T-cells together with the accumulation of CD 4- and CD 15-positive cells is the main phenomenon occurring in HNL.
Ohshima
K
K
First Department of Pathology, School of Medicine, Fukuoka University, Japan.
Kikuchi
M
M
Sumiyoshi
Y
Y
Masuda
Y
Y
Mohtai
H
H
Eguchi
F
F
Takeshita
M
M
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000302:isolation & purification; D015704:CD4 Antigens / Q000302:isolation & purification; D016827:CD8 Antigens / Q000302:isolation & purification; D002648:Child; D005260:Female; D015614:Histiocytosis / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D016256:Lewis X Antigen; D008198:Lymph Nodes / Q000473:pathology; D008199:Lymphadenitis / Q000473:pathology*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D009687:Nuclear Proteins / Q000302:isolation & purification*; D010641:Phenotype; D013997:Time Factors
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015704:CD4 Antigens; D016827:CD8 Antigens; D019394:Ki-67 Antigen; D016256:Lewis X Antigen; D009687:Nuclear Proteins