Demonstration of cross-reactive antibodies able to elicit lysis of both HIV-1- and HIV-2-infected cells.
A total of 100% of sera from a large number of HIV-1-infected patients contained antibodies able to elicit Antibody-dependent cellular cytotoxicity lysis of cells infected with the HIV-1 isolates IIIB or RF. Levels of activity could not be correlated with activities in ELISA or neutralizing antibody assays nor with the clinical status of the patients. Surprisingly, 8 of 156 patients sera could additionally elicit lysis of HIV-2-infected cells, and cold target competition assays demonstrated that the cross-reactivity was apparently mediated via recognition of common epitope(s) expressed on the surface of cells infected with either group of HIV. The ADCC mechanism was shown to be mediated by a CD16+ lymphocyte. This demonstration of an effector mechanism able to attack and eliminate cells infected with a wide range of HIV strains has obvious implications for development of putative vaccines.
Norley
S G
SG
Paul-Ehrlich-Institut, Langen, FRG.
Mikschy
U
U
Werner
A
A
Staszewski
S
S
Helm
E B
EB
Kurth
R
R
D000920:Antibody-Dependent Cell Cytotoxicity; D000943:Antigens, Differentiation / Q000032:analysis; D015153:Blotting, Western; D003429:Cross Reactions; D000939:Epitopes; D015483:HIV Antibodies / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D011961:Receptors, Fc / Q000032:analysis; D017452:Receptors, IgG
D000943:Antigens, Differentiation; D000939:Epitopes; D015483:HIV Antibodies; D015488:HIV Antigens; D011961:Receptors, Fc; D017452:Receptors, IgG