Efficient production of mouse and rat monoclonal antibodies against the O antigens of Salmonella serogroup C1, using LPS-coated bacteria as immunogen.
A variety of different immunogens and immunisation schemes were investigated for the production of monoclonal antibodies directed against the O antigenic polysaccharide of the lipopolysaccharide (LPS) of Salmonella serogroup C1 (O:6,7). Of 12 fusions performed, higher yields of stable, LPS-reactive hybridomas producing monoclonal antibodies were achieved in both the mouse and rat when using O:6,7 LPS-coated S. thompson bacteria as immunogens than with live and heat-killed bacteria, or O:6,7-BSA glycoconjugate as immunogens. All of the 17 hybrid clones obtained were shown to bind the O antigens of Salmonella serogroup C1 when tested in ELISA against a set of chemically defined LPS from Salmonella smooth and rough strains. The results are discussed with a view to bettering the immunisation strategy for production of monoclonal antibodies against the LPS antigens of bacteria.
Luk
J M
JM
Department of Clinical Bacteriology, Karolinska Institute, Huddinge Hospital, Sweden.
Nnalue
N A
NA
Lindberg
A A
AA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D006825:Hybridomas / Q000378:metabolism; D007132:Immunoglobulin Isotypes; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019081:O Antigens; D051381:Rats; D012475:Salmonella / Q000276:immunology*; D014611:Vaccination / Q000379:methods
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007132:Immunoglobulin Isotypes; D008070:Lipopolysaccharides; D019081:O Antigens