Organization of a cloned repetitive DNA fragment in mosquito genomes (Diptera: Culicidae).
The structure and genomic organization of a cloned 5.2-kb repetitive DNA fragment, H-85, isolated from the Aedes albopictus genome have been examined. In situ hybridization of the 3H-labeled H-85 DNA to the meiotic and mitotic chromosome preparations of Ae. albopictus shows that the sequences homologous to H-85 DNA are dispersed throughout the length of all three pairs of chromosomes. A similar pattern of in situ hybridization appears in Aedes seatoi, Aedes flavopictus, and Aedes aegypti. The study shows that the arrangement of sequences in the cloned 5.2-kb fragment is rare in the Ae. albopictus genome. Dot-blot hybridization reveals that the sequences homologous to H-85 DNA are present in 12 species of mosquitoes examined, belonging to six genera in subfamilies Culicinae ad Anophelinae. The H-85 sequences are also present in the genome of Mochlonyx velutinus of the nematocerous family Chaoboridae, earlier proposed as the ancestor of the mosquito family Culicidae. Although the sequences homologous to H-85 DNA are present in different species of mosquitoes, they have diverged in their structure and organization. The cloned 5.2-kb fragment is composed of elements of different and independently evolving repetitive DNA families.
Kumar
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Department of Biological Sciences, University of Notre Dame, IN 46556.
Rai
K S
KS
D000330:Aedes / Q000235:genetics; D000818:Animals; D000852:Anopheles / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D003465:Culex / Q000235:genetics; D009033:Culicidae / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D016678:Genome; D007814:Larva; D008297:Male; D008540:Meiosis; D009420:Nervous System / Q000166:cytology; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013737:Testis / Q000166:cytology
D004247:DNA