Localization of pulmonary carbonyl reductase in guinea pig and mouse: enzyme histochemical and immunohistochemical studies.
We studied the localization of carbonyl reductase (E.C. 1.1.1.184) in guinea pig and mouse lung by enzyme histochemistry and immunohistochemistry, using antibodies against the guinea pig lung enzyme which crossreacted with the lung enzymes of both animals. Carbonyl reductase activity was detectable in the bronchiolar epithelial cells of small airways and in alveolar cells. In the immunohistochemical staining for carbonyl reductase, the reaction was strongest in the non-ciliated bronchiolar cells (Clara cells) and was weak in the ciliated cells and type II alveolar pneumocytes. Injection of a single dose of naphthalene led to significant impairment of carbonyl reductase activity and of microsomal mixed-function oxidase activities in mouse lung, with a marked decrease in both activity and immunoreactive staining in the bronchiolar epithelial cells. The results indicate that carbonyl reductase is localized primarily in the Clara cells, which are known to be sites of pulmonary drug metabolism.
Department of Biochemistry, Gifu Pharmaceutical University, Japan.
K
K
Matsuura
A
A
Hara
H
H
Sawada
Y
Y
Bunai
I
I
Ohya
D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D001980:Bronchi / Q000201:enzymology / Q000648:ultrastructure; D006168:Guinea Pigs; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008168:Lung / Q000032:analysis / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors; D009281:Naphthalenes / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000201:enzymology / Q000648:ultrastructure; D013194:Staining and Labeling; D013347:Subcellular Fractions / Q000201:enzymology
D009281:Naphthalenes; C031721:naphthalene; D006899:Mixed Function Oxygenases; D000426:Alcohol Dehydrogenase