Carboxyl ester lipase: a highly polymorphic locus on human chromosome 9qter.
Carboxyl ester lipase (CEL) is a major component of pancreatic juice and is responsible for the hydrolysis of cholesterol esters as well as a variety of other dietary esters. As part of an effort to elucidate the role of this enzyme in the genetic control of lipid metabolism, we report here the chromosomal mapping of the gene for CEL to the most distal part of the long arm of human chromosome 9 using analysis of mouse-human somatic cell hybrids and in situ hybridization to chromosomes. A chromosome 9 translocation was utilized to determine the position of the CEL gene relative to various genetic markers previously localized to this region. Finally, we report that the CEL locus exhibits a high degree of polymorphism and contains a hypervariable region of the insertion/deletion variety.
Department of Medicine, University of California, Los Angeles 90024.
A K
AK
Taylor
J L
JL
Zambaux
I
I
Klisak
T
T
Mohandas
R S
RS
Sparkes
M C
MC
Schotz
A J
AJ
Lusis
D000818:Animals; D015139:Blotting, Southern; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014178:Translocation, Genetic
D015342:DNA Probes; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase