Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida.
A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60,771 Da.
Institute of Organic Chemistry, University of Karlsruhe, Germany.
M
M
Fessenmaier
R
R
Frank
J
J
Retey
C
C
Schubert
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D006639:Histidine / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D014559:Urocanate Hydratase / Q000235:genetics*
D004269:DNA, Bacterial; D006639:Histidine; D014559:Urocanate Hydratase