Histamine influences the expression of the interleukin-6 receptor on human lymphoid, monocytoid and hepatoma cell lines.
Based on our earlier data on the enhancing effect of histamine on the action of interleukin-6 (IL-6), we have studied the molecular mechanisms of these interactions. The effect of histamine was investigated on the binding of 125I-IL-6 by B lymphoma cell line CESS, monocytoid cell line U937 and hepatoma cell line HepG2. Histamine increases the IL-6 binding by CESS cells and inhibits that by U937 and HepG2 cells. Using H1 receptor (cetirizin and loderix) and H2 receptor (cimetidine and ranitidine) specific antagonists, an H1-dependent stimulation of IL-6 binding by CESS cells was found. In contrast, down-regulation of IL-6 binding by histamine was clearly mediated through H2 receptors. On U937 cells, using a monoclonal antibody reacting with the 80 kd chain of the human IL-6 receptor, and H2-receptor mediated inhibition of IL-6 receptor expression was found by FACS analysis.
Dept. of Immunology, National Institute of Rheumatology and Physiotherapy, Budapest, Hungary.
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Merétey
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Falus
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Taga
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Kishimoto
D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D005434:Flow Cytometry; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D007457:Iodine Radioisotopes; D008113:Liver Neoplasms / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D016393:Lymphoma, B-Cell / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D011969:Receptors, Histamine H1 / Q000502:physiology; D011970:Receptors, Histamine H2 / Q000502:physiology; D011971:Receptors, Immunologic / Q000378:metabolism*; D019947:Receptors, Interleukin-6; D014407:Tumor Cells, Cultured
D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D015850:Interleukin-6; D007457:Iodine Radioisotopes; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6; D006632:Histamine