[Monoclonal antibodies to bovine prolactin interacting with human prolactin].
Two stable hybridomas producing antibodies (Mab 1 and Mab 2) to bovine prolactin and belonging to the IgG1 subclass have been prepared. The cross-reactivity of Mab 1 and Mab 2 with some structurally similar pituitary protein (human, pig, whale, rat prolactins, bovine and human somatotropins) using indirect immunoenzymatic assay, was studied. It has been shown that Mab 2 reacts specifically only with bovine prolactin whereas Mab 1 interacts with human prolactin and prolactins of different animals. The specificity of Mab 1 to human prolactin was confirmed by immunoradiodetection assay on nitrocellulose filters. The data obtained give evidence of the existence of at least two different sterically nonoverlapping epitopes: one of them is specific exclusively for bovine prolactin and the other one is common, i.e. extraspecific.
Fidler
R
R
Osipova
T A
TA
Grigor'ian
A L
AL
Papazov
I P
IP
Verbitskiĭ
M Sh
MSh
Bulatov
A A
AA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D003429:Cross Reactions / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D013006:Growth Hormone / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011388:Prolactin / Q000276:immunology*; D051381:Rats; D013045:Species Specificity; D013552:Swine; D014907:Whales
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011388:Prolactin; D013006:Growth Hormone
Monoklonal'nye antitela k prolaktinu krupnogo rogatogo skota, vzaimodeĭstvuiushchie s prolaktinom cheloveka.