The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing.
Polymerase chain reaction analysis was used to identify aberrant splicing of the simian virus 40 small-t intron present in pRSVcat. We examined factors governing the selection and relative use of aberrant 5' splice sites derived from the chloramphenicol acetyltransferase-coding region. Our results indicated that transcripts from virtually any cDNA positioned upstream of the small-t intron could contain alternative 5' splice sites and therefore be subject to deletions within the protein-coding region.
Huang
M T
MT
Department of Cell Genetics, Genentech Inc., South San Francisco, California 94080.
Gorman
C M
CM
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D005822:Genetic Vectors; D007438:Introns; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics; D012326:RNA Splicing; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D015345:Oligonucleotide Probes; D012313:RNA