Identification of a rat liver dipeptidyl aminopeptidase IV with a liver plasma membrane glycoprotein (gp110). A study using dipeptidyl aminopeptidase IV-deficient rats.
A rat liver plasma membrane glycoprotein, gp110, was compared with dipeptidyl aminopeptidase IV (DAP IV) by using Wistar rats (DAP IV-positive rats) and Fischer 344 rats (DAP IV-negative rats). Fischer rats also lacked gp110 and gp110 of Wistar rats had DAP IV activity. Furthermore, we showed that the C-terminal sequence of gp110 was Ser-Leu-Arg, which was the same as the C-terminal amino acid sequence deduced from the nucleotide sequence of the cDNA of DAP IV. According to these results, we concluded that gp110 was identical with DAP IV.
Iwaki-Egawa
S
S
Department of Clinical Biochemistry, Hokkaido Institute of Pharmaceutical Sciences, Japan.
Watanabe
Y
Y
Fujimoto
Y
Y
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000172:deficiency; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000737:chemistry* / Q000172:deficiency; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D016415:Sequence Alignment
D008562:Membrane Glycoproteins; C057630:canaliculus domain-specific glycoprotein gp110; D000626:Aminopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4