Immunoelectron microscopic distribution of actin in hamster spermatids and epididymal, capacitated and acrosome-reacted spermatozoa.
The distribution of actin in hamster sperm cells was studied during spermiogenesis, epididymal transit, in vitro capacitation and acrosome reaction by immunogold procedures using a polyclonal and two monoclonal antiactin antibodies. A predominant actin labeling (F-actin) was detected in the subacrosomal space of spermatids. Actin labeling was also observed under the plasma membrane of intercellular bridges and along the outer acrosomal membrane. In late spermatids there was both F-actin depolymerization and a loss of actin immunolabeling, thus suggesting a dispersion of G-actin monomers. No obvious labeling was evidenced in residual bodies. This pattern was observed with the three antiactin probes. In contrast, an actin labeling reappeared over the fibrous sheath of the flagellum in epididymal spermatozoa but only when the polyclonal antibody was used. Only one single actin reactive band was detected by immunoblotting of sperm extracts. Since the sperm tails were NBD phallacidin negative they were considered to contain either G-actin or actin oligomers rather than bundles of actin filaments. It is suggested that G-actin originating in the head of late spermatids was redistributed to the flagellum of epidymal spermatozoa. No further changes were noted after capacitation and acrosome reaction thus indicating no apparent effect on actin polymerization and distribution.
Département de Cytologie et Histologie, UFR Biomédicale des Saints-Pères, Paris, France.
J P
JP
Fouquet
M L
ML
Kann
J P
JP
Dadoune
D000177:Acrosome / Q000737:chemistry* / Q000473:pathology / Q000648:ultrastructure; D000199:Actins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000737:chemistry* / Q000473:pathology / Q000648:ultrastructure; D006046:Gold; D015151:Immunoblotting; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D013075:Sperm Capacitation / Q000502:physiology*; D013077:Sperm Head / Q000737:chemistry / Q000648:ultrastructure; D013082:Sperm Tail / Q000737:chemistry / Q000648:ultrastructure; D013087:Spermatids / Q000737:chemistry* / Q000473:pathology / Q000648:ultrastructure; D013091:Spermatogenesis / Q000502:physiology; D013194:Staining and Labeling / Q000379:methods
D000199:Actins; D000906:Antibodies; D006046:Gold