CD of the synthetic RNA duplexes poly[r(A-T)] and poly[r(A-U)] in salt and ethanolic solutions.
Synthetic RNA poly[r(A-T)] has been synthesized and its CD spectral properties compared to those of poly[r(A-U)], poly[d(A-T)], and poly[d(A-U)] in various salt and ethanolic solutions. The CD spectra of poly[r(A-T)] in an aqueous buffer and of poly[d(A-T)] in 70.8% v/v ethanol are very similar, suggesting that they both adopt the same A conformation. On the other hand, the CD spectra of poly[r(A-T)] and of poly[r(A-U)] differ in aqueous, and even more so in ethanolic, solutions. We have recently observed a two-state salt-induced isomerization of poly[r(A-U)] into chiral condensates, perhaps of Z-RNA [M. Vorlícková, J. Kypr, and T. M. Jovin, (1988) Biopolymers 27, 351-354]. It is shown here that poly[r(A-T)] does not undergo this isomerization. Both the changes in secondary structure and tendency to aggregation are different for poly[r(A-T)] and poly[r(A-U)] in aqueous salt solutions. In most cases, the CD spectrum of poly[r(A-U)] shows little modification of its CD spectrum unless the polymer denatures or aggregates, whereas poly[r(A-T)] displays noncooperative alterations in its CD spectrum and a reduced tendency to aggregation. At high NaCl concentrations, poly[r(A-T)] and poly[r(A-U)] condense into psi(-) and psi(+) structures, respectively, indicating that the type of aggregation is dictated by the polynucleotide chemical structure and the corresponding differences in conformational properties.
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
M
M
Vorlícková
J
J
Kypr
T M
TM
Jovin
M
M
Planck
D000643:Ammonium Chloride; D001482:Base Composition; D002942:Circular Dichroism; D000431:Ethanol; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides; D009994:Osmolar Concentration; D011061:Poly A; D011063:Poly A-U; D011071:Poly T; D012313:RNA; D012996:Solutions; D013997:Time Factors
D009843:Oligoribonucleotides; D012996:Solutions; D000643:Ammonium Chloride; D011063:Poly A-U; D011061:Poly A; D011071:Poly T; C008950:poly A-T; D000431:Ethanol; D012313:RNA