Microcarrier cell culture of neonatal human corneal endothelium.
Fresh isolates of neonatal human corneal endothelial cells were maintained in tissue culture using a technique employing collagen-coated, dextran-based microcarrier beads. This method provides large yields of endothelial cells suitable for biochemical and/or transplant studies without exposing the cells to enzymatic passage. Our results suggest that microcarrier culture of human corneal endothelial cells can provide a useful in vitro system for the growth and maintenance of actively mitotic cells.
Department of Ophthalmology, Louisiana State University Medical Center School of Medicine, New Orleans 70112.
M S
MS
Insler
J G
JG
Lopez
D002452:Cell Count; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003911:Dextrans; D004728:Endothelium, Corneal / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D007231:Infant, Newborn; D008722:Methods; D008855:Microscopy, Electron, Scanning; D008863:Microspheres
D003911:Dextrans