Neurotransmitter amino acid variations in striatum of rats exposed to 50 Hz electric fields.
Concentration of neurotransmitter amino acids (Tau, Gly, Asp, Glu, Gln, Ala, GABA) were measured in rat striatum following varying exposure (320 to 1408 h) to high intensity (50 Hz) electric fields. Tissue extracts in methanol, after drying, were derivatized with dansyl chloride and the amino acids quantitated by high-pressure liquid chromatography with ultraviolet detection. Short exposures (320 h) to 100 kV/m field induced a decrease in almost all tested amino acids. Longer exposure times (640 h) to 25 and 100 kV/m were only associated with a decrease in Tau. A further increase of the exposure time (1240 and 1408 h) both at 25 and 180 kV/m were mainly associated with a reduction of the amino acid levels. It is concluded that electric fields in the range 20-180 kV/m generate bimodal variations in neurotransmitter amino acids with troughs at very short and longer exposure times, independent from the field strength.
Zecca
L
L
Istituto di Tecnologie Biomediche Avanzate, CNR, Milano, Italy.
Ferrario
P
P
Margonato
V
V
Cerretelli
P
P
Zonta
N
N
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000378:metabolism*; D003619:Dansyl Compounds / Q000737:chemistry; D004560:Electricity; D008297:Male; D000432:Methanol / Q000737:chemistry; D018377:Neurotransmitter Agents / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D003619:Dansyl Compounds; D018377:Neurotransmitter Agents; D000432:Methanol