Testicular macromolecular synthesis in men with varicocele.
The synthesis of DNA, RNA, and protein by varicocele testes with spermatogenic dysfunction was studied by examination of the levels of incorporation of [3H]thymidine, [14C]uridine, and [14C]leucine into the testicular tissue at 31 degrees C in vitro. The results suggest that DNA synthesis by the testis with moderate tubular damage is impaired whereas synthesis of RNA and protein is not. The cell regenerative ability of the testis with moderate tubular damage may be poor in spite of the preservation of testicular cell RNA and protein synthesis.
Nakamura
M
M
Department of Urology, Osaka University Hospital, Japan.
Koh
E
E
Kondoh
N
N
Takeyama
M
M
Namiki
M
M
Fujioka
H
H
Kiyohara
H
H
Nishimune
Y
Y
Okuyama
A
A
Sonoda
T
T
D000328:Adult; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012671:Seminiferous Tubules / Q000473:pathology*; D013737:Testis / Q000378:metabolism* / Q000473:pathology*; D014646:Varicocele / Q000378:metabolism* / Q000473:pathology
D012313:RNA; D004247:DNA