Adhesion of human B cells to germinal centers in vitro involves VLA-4 and INCAM-110.
Human B lymphocytes localize and differentiate within the microenvironment of lymphoid germinal centers. A frozen section binding assay was developed for the identification of those molecules involved in the adhesive interactions between B cells and lymphoid follicles. Activated human B cells and B cell lines were found to selectively adhere to germinal centers. The VLA-4 molecule on the lymphocyte and the adhesion molecule INCAM-110, expressed on follicular dendritic cells, supported this interaction. This cellular interaction model can be used for the study of how B cells differentiate.
Division of Tumor Immunology, Dana-Farber Cancer Institute and Boston, MA.
A S
AS
Freedman
J M
JM
Munro
G E
GE
Rice
M P
MP
Bevilacqua
C
C
Morimoto
B W
BW
McIntyre
K
K
Rhynhart
J S
JS
Pober
L M
LM
Nadler
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002478:Cells, Cultured; D006801:Humans; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology; D016029:Receptors, Very Late Antigen / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D019010:Vascular Cell Adhesion Molecule-1
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D016029:Receptors, Very Late Antigen; D019010:Vascular Cell Adhesion Molecule-1