Chromogenic assay for equine plasminogen.
A functional assay for equine plasminogen was established, using urokinase as the activator, a synthetic chromogenic substrate, a computer-assisted centrifugal analyzer, and acidified/neutralized plasma. One documented effect of plasma acidification appears to be inactivation of alpha-2-antiplasmin. Intra- and interassay precision testing yielded coefficients of variation of 4.1% (n = 10) and 5.6% (n = 26), respectively. Plasminogen was stable in equine plasma stored up to 1 week at 4 C and up to 5 months at -70 C. Plasminogen in nonacidified equine plasma was not activated by urokinase, streptokinase, tissue plasminogen activator, or tissue plasminogen activator plus soluble fibrin. Streptokinase also failed to activate plasminogen in acidified/neutralized equine plasma.
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602.
E G
EG
Welles
K W
KW
Prasse
A
A
Duncan
D000818:Animals; D002863:Chromogenic Compounds; D005341:Fibrinolysin / Q000032:analysis; D006736:Horses / Q000097:blood*; D010958:Plasminogen / Q000032:analysis*; D013300:Streptokinase / Q000097:blood / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000097:blood / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000032:analysis
D002863:Chromogenic Compounds; D000511:alpha-Macroglobulins; C024668:plasmin-alpha(2)-macroglobulin complex; D010958:Plasminogen; D013300:Streptokinase; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator