Immunosuppressive activity of staphylococcal enterotoxin B. I. Characterization of staphylococcal enterotoxin-B-induced suppressor cells.
Staphylococcal enterotoxin B (SEB) binds specifically to major histocompatibility complex class II molecules on the surface of accessory cells and stimulates virtually all T cells bearing certain, but not all, T cell-receptor V beta alleles. We have previously shown that this superantigen is a potent inducer of multiple regulatory T cell populations. In the present report we show that SEB induces a population of suppressor T cells which inhibits the generation of alloantigen-induced cytotoxic T cell activity. Using both negative- and positive-selection analysis, we found that this suppressor population is a CD4- CD8- CD5+ IL-2R+ T cell. This cell population inhibited both syngeneic and allogeneic cytotoxic T lymphocyte activity, but the cell population which inhibited allogeneic CTL activity was radiation sensitive. In addition, allogeneic SEB-primed cells appeared to develop cytolytic activity as a result of the additional stimulation in the mixed-lymphocyte reaction culture. The relationship of the SEB-primed CD4- CD8- CD5+ T cells to related regulatory T cell populations is discussed.
Patel
M
M
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Taub
D D
DD
Lin
Y S
YS
Rogers
T J
TJ
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D018956:CD5 Antigens; D016827:CD8 Antigens; D004768:Enterotoxins / Q000276:immunology*; D016130:Immunophenotyping; D066298:In Vitro Techniques; D007519:Isoantigens / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D018800:Thy-1 Antigens
D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D015704:CD4 Antigens; D018956:CD5 Antigens; D016827:CD8 Antigens; D004768:Enterotoxins; D007519:Isoantigens; D018800:Thy-1 Antigens; C031818:enterotoxin B, staphylococcal