[New alloantisera to antigenic determinant I--J, their specificity and effect on the course of tuberculous infection in mice].
Preparations of anti-I--J alloantisera with higher activity were obtained by a new method, based on induction of expression of the I--J determinant in donor suppressor cells, immunization of mice with such cells, and subsequent elimination of contaminating anti-idiotypic antibodies from antisera by their adsorption on the cells of the recipient murine strain sharing induced idiotype with the donor cells. After adsorption anti-I--J activity remained unchanged. The antisera thus obtained were found active in the standard cytotoxic assay (working dilution greater than or equal to 1:20). The injection of anti-I--Jk antiserum to CBA mice prolonged the survival time of the animals and increased the parameters of the cell-mediated immunity after infection with Mycobacterium tuberculosis virulent strain H37Rv.
Z M
ZM
Batridinova
G A
GA
Vakhidova
A M
AM
Moroz
A S
AS
Apt
D000483:Alleles; D000818:Animals; D004353:Drug Evaluation, Preclinical; D000939:Epitopes / Q000276:immunology*; D005838:Genotype; D007106:Immune Sera / Q000276:immunology* / Q000302:isolation & purification; D007108:Immune Tolerance / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D007114:Immunization / Q000379:methods; D007116:Immunization, Passive; D007518:Isoantibodies / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008815:Mice, Inbred Strains; D013997:Time Factors; D014376:Tuberculosis / Q000276:immunology* / Q000628:therapy
D000939:Epitopes; D007106:Immune Sera; D007518:Isoantibodies
Novye alloantisyvorotki k antigennoĭ determinante I--J, ikh spetsifichnosti i vliianie na techenie tuberkuleznoĭ infektsii u mysheĭ.