K-ras oncogene mutations in neoplasias of kidney transplanted patients: preliminary results with a new technique.
K-ras oncogene activations by point mutations are frequent in many forms of human cancers but there is a special category of cancers occurring in immunosuppressed patients after kidney transplantation in which the frequency of K-ras oncogene activation has not been fully studied. We used a new sensitive and easy method for the detection of this mutation, and in 8 DNA samples studied from various neoplasias of 8 patients after kidney transplantation, we found 4 mutations. Our preliminary results indicate that the activation of K-ras oncogene at codon 12, is a common event among the kidney transplanted patients who present a neoplasia, even in the least aggressive forms of the disease, contrary to the sporadic cases.
Skalkeas
G D
GD
Laikon Hospital, Medical School, University of Athens, Greece.
Spandidos
D A
DA
Kostakis
A
A
Balafouta-Tseleni
S
S
Choremi
E
E
Iliopoulos
D
D
Haliassos
A
A
D000328:Adult; D000595:Amino Acid Sequence; D003062:Codon / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D007108:Immune Tolerance; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity
D003062:Codon