[Nucleic acid hybridization for direct detection of Chlamydia trachomatis. Comparison of a radioactive with a non-radioactive procedure].
A total of 234 urogenital specimens were investigated with a radioactive and a nonradioactive rRNA:cDNA-hybridization test for the direct detection of Chlamydia trachomatis (Gen-Probe, San Diego, Calif.). The results were compared with those yielded by the conventional cell culture technique (McCoy cells). Using cell culture a urogenital C. trachomatis infection could be detected in 37 of 234 patients (15.8%) at two outpatient sexually transmitted disease clinics. Referred to the detection rate, cell culture sensitivity was 83.8% for the radioactive test and 86.5% for the nonradioactive DNA-hybridization test. The specificity of the radioactive test was 94.8% and that of the nonradioactive test, 93.4%.
Hautklinik, Ruprecht-Karls-Universität Heidelberg.
H
H
Näher
B
B
Niebauer
M
M
Hartmann
J
J
Söltz-Szöts
D
D
Petzoldt
D002478:Cells, Cultured; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification; D015342:DNA Probes; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D012329:RNA, Bacterial / Q000032:analysis; D014526:Urethritis / Q000175:diagnosis*; D002575:Uterine Cervicitis / Q000175:diagnosis*
D015342:DNA Probes; D007457:Iodine Radioisotopes; D012329:RNA, Bacterial
Nukleinsäure-Hybridisierung zum Direktnachweis von Chlamydia trachomatis. Vergleich eines radioaktiven mit einem nicht-radioaktiven Verfahren.