A molecular genetic linkage map of mouse chromosome 19, including the lpr, Ly-44, and Tdt genes.
The mouse lpr gene, which is an autosomal recessive gene causing autoimmune disease with features of human systemic lupus erythematosus and eventually death from severe immune-complex glomerulonephritis, has been mapped on chromosome 19. To determine its exact chromosomal location, a three-point backcross was carried out by mating (MRL/MpJ-lpr/lpr x MOL-MIT)F1 x MRL/MpJ-lpr/lpr using the genes Ly-44 (lymphocyte differentiation antigen-44) and Tdt (terminal deoxynucleotidyl transferase) as markers. The following order of genes is proposed, with the distances between genes given in parentheses: centromere-Ly-44 (19.3 cM)-lpr (6.1 cM)-Tdt-telomere. The Ly-44a and Tdta alleles are found in all laboratory strains and in the wild Western European subspecies, domesticus and brevirostris. In contrast, the Ly-44b and Tdtb alleles are found in some Asian subspecies, Chinese mice of wild origin, yamashinai and molossinus. Furthermore the third Tdt allele, Tdtc, is detected in castaneus.
Watanabe
T
T
Department of Biochemistry, Aichi Prefecture Colony, Japan.
Sakai
Y
Y
Miyawaki
S
S
Shimizu
A
A
Koiwai
O
O
Ohno
K
K
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics*; D000950:Antigens, Ly / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes; D003433:Crosses, Genetic; D004253:DNA Nucleotidylexotransferase / Q000235:genetics*; D015342:DNA Probes; D005808:Genes, Recessive; D008040:Genetic Linkage; D008232:Lymphoproliferative Disorders / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D000944:Antigens, Differentiation, B-Lymphocyte; D000950:Antigens, Ly; D015342:DNA Probes; C057962:LY 44 antigen; D004253:DNA Nucleotidylexotransferase