Specific synthetic peptides for detection of and discrimination between HIV-1 and HIV-2 infection.
A panel of highly purified synthetic oligopeptides representing defined parts of the gag and env proteins of HIV-1 and HIV-2 were used as antigens in ELISA for serodiagnosis of HIV-1 and HIV-2 infection. The analysis included sera from 321 HIV-infected patients and 201 healthy controls from the Ivory Coast, where the prevalence is high for both HIV-1 and HIV-2, and sera from European HIV-1-infected individuals. All sera from HIV-1-infected individuals reacted with a 20 amino acid (a.a.) peptide JB-4c (a.a. 594-613) derived from a highly immunogenic conserved region of the external part of gp41. An equally good response was seen in the HIV-2-infected individuals to a 20 a.a. peptide, JB-16c, from the corresponding part of HIV-2 gp36. Both HIV-1- and HIV-2-seropositive individuals responded well to a peptide, JB-8pc (a.a. 427-448), representing the C-terminal end of the putative CD4-binding site of gp120 of HIV-1. The frequency of reactivity to three selected HIV-1 gag peptides derived from p17 and p15 was 60-70% in both HIV-1 and HIV-2-positive sera. To distinguish between HIV-1 and HIV-2 infection, the sera were titrated against the peptides. Although there was a high degree of cross-reactivity at lower serum dilutions, it was possible to discriminate the infections at higher dilutions to the HIV-1 and HIV-2 gp41/gp36 peptides JB-4c and JB-16c. Analysis of serum reactivity to several selected peptides thus allowed the identification of HIV infection, and the discrimination between HIV-1 and HIV-2.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Virology, Karolinska Institute, Stockholm, Sweden.
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Broliden
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Rudén
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Ouattara
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de Thé
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Sølver
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Trojnar
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Wahren
D000354:Africa, Western / Q000453:epidemiology; D000595:Amino Acid Sequence; D006800:Deltaretrovirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D003937:Diagnosis, Differential; D005060:Europe / Q000453:epidemiology; D015686:Gene Products, env; D015683:Gene Products, gag / Q000276:immunology; D015483:HIV Antibodies / Q000097:blood; D006679:HIV Seropositivity / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D015498:HIV-2 / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology
D015686:Gene Products, env; D015683:Gene Products, gag; D015483:HIV Antibodies; D010455:Peptides