Multiple hormone actions: the rises in cAMP and Ca++ in MDCK-cells treated with glucagon and prostaglandin E1 are independent processes.
Glucagon and prostaglandin E1 stimulate adenylate cyclase in Madin-Darby canine kidney cells with an approximate EC50 of 3*10(-8) and 1*10(-7) M respectively. The rise in cAMP is accompanied by a transient rise in intracellular Ca++ measured with the fluorescent calcium indicator Indo-1. A comparable increase in intracellular Ca2+ without a rise in cAMP occurs with the cholinergic agonist carbamylcholine. Stimulation of adenylate cyclase by the beta-adrenergic agonist isoproterenol or directly by forskolin has no effect on intracellular Ca++. By all criteria studied the rise in intracellular Ca++ and the increase in cAMP are independent from each other.
Department of Physiological Chemistry, University of Würzburg Medical School, F.R.G.
N P
NP
Kurstjens
H
H
Heithier
R C
RC
Cantrill
M
M
Hahn
F
F
Boege
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004285:Dogs; D005456:Fluorescent Dyes; D005934:Glucagon / Q000378:metabolism / Q000494:pharmacology*; D007211:Indoles; D007545:Isoproterenol / Q000494:pharmacology; D007668:Kidney; D007700:Kinetics; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism; D018027:Receptors, Glucagon
D005456:Fluorescent Dyes; D007211:Indoles; D011964:Receptors, Gastrointestinal Hormone; D018027:Receptors, Glucagon; D005576:Colforsin; D002217:Carbachol; D005934:Glucagon; D000242:Cyclic AMP; D000527:Alprostadil; D007545:Isoproterenol; C048960:indo-1; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine