Golgi-like demonstration of "dark" neurons with an argyrophil III method for experimental neuropathology.
A silver method is proposed for the selective, well-contrasted and reproducible demonstration of "dark" neurons in frozen, vibratome and paraffin sections cut at a thickness of 5 to 200 microns from aldehyde-fixed brains. The Golgi-like staining of the dendrites enables assorting of "dark" neurons according to characteristic neuron classifications. The staining procedure includes an esterification with 1-propanol, a treatment with diluted acetic acid and development. The esterification strongly increases the argyrophilia of both "dark" neurons and mitochondria. Unwanted co-staining of mitochondria is suppressed by the acetic acid treatment, while a special developer is used to render the staining controllable. The applicability of the method to experimental neuropathology is demonstrated by Golgi-like staining of "dark" neurons in rat brains exposed, before transcardial perfusion-fixation and delayed autopsy, to various pathological conditions including ischemia, hypoglycemia, trauma, status epilepticus, deafferentation and poisoning with kainic acid, colchicine and sodium azide, respectively.
Department of Neurosurgery, University of Pécs, Hungary.
F
F
Gallyas
F H
FH
Güldner
G
G
Zoltay
J R
JR
Wolff
D000818:Animals; D001927:Brain Diseases / Q000473:pathology*; D002415:Cats; D005260:Female; D005849:Gerbillinae; D006652:Histological Techniques; D008297:Male; D051379:Mice; D011817:Rabbits; D051381:Rats; D012834:Silver; D013194:Staining and Labeling; D013552:Swine
D012834:Silver