[A method of separating mononuclears on a density gradient].
The developed procedure for the isolation of mononuclears in density gradient d = 1.0077 g/cm3 consists in separation of the plasma by preliminary centrifugation at 200 g for 7-10 min and isolation of leukocytic mononuclear cells from the cellular sediment diluted with the isotonic solution. The length of centrifugation at 400 g is selected with consideration for the thickness of the density gradient. An anticoagulant is added to the isotonic solution and ficoll-verografin mixture to improve the quality of the cell isolation.
A S
AS
Prilutskiĭ
A V
AV
Khodakovskiĭ
E A
EA
Maĭlian
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*
Metod vydeleniia mononuklearov v gradiente plotnosti.