Antibodies to the beta-amyloid peptide cross-react with conformational epitopes in human fibrinogen subunits from peripheral blood.
Antibodies to the Alzheimer disease (AD) beta-amyloid peptide (beta AP) were used to identify beta AP precursor fragments in blood. The antibodies detected 3 major polypeptides with apparent molecular weights (MW) of 47-64,000 in Western blots of plasma derived clot proteins, but these proteins corresponded to human A-alpha, B-beta and gamma-fibrinogen since they reacted with 2 different anti-fibrinogen antisera, and the anti-beta AP and anti-fibrinogen antibodies recognized purified fibrinogen and fibrin. These data are significant for efforts to develop immunochemical assays to diagnose and monitor the progression of AD.
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19104.
R A
RA
Stern
J Q
JQ
Trojanowski
V M
VM
Lee
D000310:Adrenal Gland Neoplasms / Q000032:analysis; D000544:Alzheimer Disease / Q000378:metabolism; D000682:Amyloid / Q000032:analysis / Q000276:immunology*; D016229:Amyloid beta-Peptides; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D001923:Brain Chemistry; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D005340:Fibrinogen / Q000276:immunology*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000276:immunology*; D010673:Pheochromocytoma / Q000032:analysis; D011487:Protein Conformation; D051381:Rats
D000682:Amyloid; D016229:Amyloid beta-Peptides; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes; D009419:Nerve Tissue Proteins; D005340:Fibrinogen