Localization of Mn-superoxide dismutase (Mn-SOD) in cholinergic and somatostatin-containing neurons in the rat neostriatum.
We used rabbit antisera against manganese (Mn)-superoxide dismutase for immunohistochemical studies of localization in the rat neostriatum. Immunostaining was intense in large-sized neurons and several medium-sized neurons, but it was moderate to weak in other cells. Double immunostaining with monoclonal antibody to choline acetyltransferase or somatostatin demonstrated large-sized, Mn-SOD immunoreactive neurons to be cholinergic, and some medium-sized neurons which were intensely immunoreactive for Mn-SOD to contain somatostatinergic.
Department of Anatomy, Osaka City University Medical School, Japan.
S
S
Inagaki
K
K
Suzuki
N
N
Taniguchi
H
H
Takagi
D000818:Animals; D000911:Antibodies, Monoclonal; D002421:Caudate Nucleus / Q000166:cytology / Q000201:enzymology*; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D008297:Male; D009474:Neurons / Q000166:cytology / Q000201:enzymology*; D011699:Putamen / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000032:analysis*; D013482:Superoxide Dismutase / Q000032:analysis*; D013861:Thiocyanates
D000911:Antibodies, Monoclonal; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D013004:Somatostatin; D013482:Superoxide Dismutase; D002795:Choline O-Acetyltransferase; D016650:Fluorescein-5-isothiocyanate