Structural and immunological characterization of a linear virus-neutralizing epitope of the rabies virus glycoprotein and its possible use in a synthetic vaccine.
We have mapped a linear epitope recognized by the virus-neutralizing monoclonal antibody 6-15C4 within the primary sequence of the G protein from the Evelyn-Rokitnicki-Abelseth strain of rabies virus. This was accomplished by using fragments of the rabies virus G protein and deduced amino acid sequences of neutralization-resistant variant rabies viruses. The monoclonal antibody 6-15C4 specifically recognized a synthetic peptide (peptide G5-24) which resembles the 6-15C4 epitope in structure. In addition, a tandem peptide constructed from the G5-24 peptide and a dominant TH cell epitope of the rabies virus N protein induced protective immunity against lethal rabies virus challenge infection in mice.
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104-4268.
B
B
Dietzschold
M
M
Gore
D
D
Marchadier
H S
HS
Niu
H M
HM
Bunschoten
L
L
Otvos
W H
WH
Wunner
H C
HC
Ertl
A D
AD
Osterhaus
H
H
Koprowski
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation; D000956:Antigens, Viral; D001483:Base Sequence; D000939:Epitopes / Q000032:analysis / Q000235:genetics*; D005814:Genes, Viral; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010455:Peptides / Q000138:chemical synthesis; D012367:RNA, Viral / Q000235:genetics; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D015678:Viral Structural Proteins / Q000235:genetics
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D010455:Peptides; D012367:RNA, Viral; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C064951:glycoprotein G, Rabies virus