A deletion and a rearrangement distinguish between the intracisternal A-particle of Hox-2.4 and that of interleukin-3 in the same leukemic cells.
Two intracisternal A-particle (IAP) insertions have been identified in WEHI-3B myeloid leukemic cells, one at the interleukin-3 (IL-3) gene and another at the homeobox gene Hox-2.4. In contrast to the 5-kb IL-3-IAP, the Hox-2.4-IAP is only 2.1 kb in size and contains a rearrangement. The homology throughout the remaining sequences suggests that both IAPs originated from a common progenitor molecule. Both proviral insertions have resulted in transcriptional activation of the adjacent genes, which appears to be a significant step in the leukemogenic process in these leukemic cells.
Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.
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Ben-David
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Aberdam
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Sachs
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Blatt
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics; D015321:Gene Rearrangement; D005801:Genes, Homeobox; D007426:Genes, Intracisternal A-Particle / Q000235:genetics*; D007377:Interleukin-3 / Q000235:genetics*; D007951:Leukemia, Myeloid / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D015183:Restriction Mapping
D004273:DNA, Neoplasm; D007377:Interleukin-3