Regulation of brain-specific transcription of the mouse myelin basic protein gene: function of the NFI-binding site in the distal promoter.
We investigated brain-specific transcription elements in the distal region (-253 to -54) of the mouse myelin basic protein (MBP) promoter by in vitro transcription using mouse brain nuclear extracts. Using deletion and base substitution mutants, we identified one tissue-specific transcription element at the downstream core of the NFI-site between -115 and -111. Foot-printing assay demonstrated that three transcription factors bind around this element; NFI-related (-130 to -111), M1 (-110 to -97) and Sp1 (-92 to -84). NFI-related factor(s) in brain extracts consisted of a characteristic population which was different from that in other tissues. Methylation interference experiments revealed that this factor(s) interacted with the NFI-downstream core in a brain-specific manner. We suggest the existence of a brain-specific NFI-related factor(s).
Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, Japan.
A
A
Aoyama
T A
TA
Tamura
K
K
Mikoshiba
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D022762:CCAAT-Enhancer-Binding Proteins; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I; D005796:Genes; D008099:Liver / Q000378:metabolism; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000235:genetics*; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D009710:Nucleotide Mapping; D011401:Promoter Regions, Genetic; D051381:Rats; D014157:Transcription Factors; D014158:Transcription, Genetic; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D004676:Myelin Basic Protein; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D003850:Deoxyribonuclease I