The herpes simplex virus trans-activator VP16 recognizes the Oct-1 homeo domain: evidence for a homeo domain recognition subdomain.
The homeo domain of the Oct-1 transcription factor directs formation of a multiprotein-DNA complex containing Oct-1, the herpes simplex virus (HSV) trans-activator VP16, and a second host cell factor (HCF). This VP16-induced complex alters the regulatory activity of Oct-1, in part, by associating it with the potent VP16 acidic transcriptional activation domain. Here, we show that in the absence of HCF, VP16 can recognize specifically the Oct-1 homeo domain. A region of VP16 near the acidic activation domain appears to be involved exclusively in homeo domain recognition because a 4-amino-acid insertion within this region only affects the ability of VP16 to interact with Oct-1, leaving its DNA- and HCF-binding activities unchanged. A 33-amino-acid peptide containing this region complexes with the Oct-1 POU domain bound to DNA, suggesting that this VP16 region contains an autonomous homeo domain recognition subdomain.
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Cold Spring Harbor Laboratory, New York 11724.
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics*; D005801:Genes, Homeobox; D017405:Herpes Simplex Virus Protein Vmw65; D051863:Host Cell Factor C1; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D009154:Mutation; D050811:Octamer Transcription Factor-1; D018139:Simplexvirus / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
D002352:Carrier Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D017405:Herpes Simplex Virus Protein Vmw65; D051863:Host Cell Factor C1; D034982:Integration Host Factors; D050811:Octamer Transcription Factor-1; D015534:Trans-Activators; D014157:Transcription Factors; D014764:Viral Proteins