Characterization of in vivo-activated T cell clones from peripheral blood of multiple sclerosis patients.
In vivo-activated interleukin-2 responsive T cell clones were generated from peripheral blood (PBL) of multiple sclerosis patients (MS) and normal subjects (N) by limiting dilution analysis. The frequency with which interleukin-2 responsive cells were cloned from PBL was higher in MS than N. CD8 was the predominant phenotype expressed by both MS (85%) and N (89%) clones. Seven clones from four MS patients but none from five N subjects specifically proliferated against myelin basic protein. These studies demonstrate the existence of MBP-reactive T cells in PBL of MS patients.
Ofosu-Appiah
W
W
Department of Medicine, Maimonides Medical Center, New York, New York.
Mokhtarian
F
F
Miller
A
A
Grob
D
D
D000954:Antigens, Surface / Q000235:genetics; D002999:Clone Cells / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D009103:Multiple Sclerosis / Q000097:blood*; D004676:Myelin Basic Protein / Q000494:pharmacology; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology*
D000954:Antigens, Surface; D007376:Interleukin-2; D004676:Myelin Basic Protein