Detection of tomato spotted wilt virus using monoclonal antibodies and riboprobes.
The immunoreactivity of a panel of monoclonal antibodies raised to tomato spotted wilt virus (TSWV) was examined in enzyme-linked immunosorbent assays (ELISA) and dot immunobinding assays (DIBA) procedures. MAbs 6.12.15 and 2.9 were specific for the nucleocapsid protein of TSWV. The sensitivity of the two immunoassays was compared with that of a dot-blot hybridization technique using riboprobes (RNA transcripts) to TSWV M RNA. Using deproteinized plant extracts or purified virus preparations, as little as 1 pg RNA could be detected. Although an ELISA using MAb 6.12.15, a DIBA procedure using MAb 3.22.6 and the dot-blot hybridization, detected several TSWV isolates in different host species equally well, the ELISA was most precise and most suitable for routine diagnosis in the field.
Department of Virology, Agricultural University, Wageningen, The Netherlands.
C
C
Huguenot
G
G
van den Dobbelsteen
P
P
de Haan
C A
CA
Wagemakers
G A
GA
Drost
A D
AD
Osterhaus
D
D
Peters
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D015151:Immunoblotting / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000276:immunology*; D012313:RNA / Q000235:genetics; D015347:RNA Probes
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D015347:RNA Probes; D012313:RNA