Positive and negative elements regulate human interleukin 3 expression.
The human interleukin 3 (IL-3) promoter is comprised of several cis-acting DNA sequences that modulate T-cell expression of IL-3. These are located within 315 nucleotides upstream of the mRNA start site. Transient expression of reporter genes linked to serially deleted sequences of the IL-3 promoter has allowed mapping of two activator sequences and an interposed repressor sequence. The proximal regulatory region is specific to IL-3 and prerequisite for efficient transcription. Its effect is enhanced by a second, more distal activating sequence consisting of an AP-1 binding site. Between the two activators lies a transcriptional silencer, which is a potent repressor in the absence of the AP-1 site. DNA-nuclear protein binding experiments demonstrate specific complex formation within each of these functional regions. Thus, both positive and negative regulatory elements appear to control expression of the human IL-3 gene in activated T cells.
Division of Hematology Oncology, Children's Hospital, Boston MA 02115.
B
B
Mathey-Prevot
N C
NC
Andrews
H S
HS
Murphy
S G
SG
Kreissman
D G
DG
Nathan
D001483:Base Sequence; D002460:Cell Line; D003850:Deoxyribonuclease I; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D007377:Interleukin-3 / Q000235:genetics*; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012260:Ribonucleases; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
D007377:Interleukin-3; D015345:Oligonucleotide Probes; D012313:RNA; D012260:Ribonucleases; D003850:Deoxyribonuclease I