Release of O2- and LTC4 by murine eosinophils: role of intra- and extracellular calcium.
Using an experimental model of mouse peritoneal eosinophilia, we investigated the role of Ca2+ in the in vitro activation of these cells challenged with specific Mesocestoides corti antigen. We have detected LTC4, a metabolite derived from arachidonic acid by way of 5'lipo-oxygenase and superoxide anion from the oxidative burst, as inflammatory mediators produced by activated eosinophils. Preincubation with hyperimmune mice serum increases the amount of LTC4 and superoxide anion in response to the antigenic extract. Release of O2- is inhibited by Verapamil (a voltage-gated calcium channel) and Quin 2 (an intracellular trapped chelator of calcium). Also, LTC4 produced by preincubated eosinophils challenged with M. corti is dramatically inhibited by Quin 2. Our results suggest an intact mechanism for calcium control for the release of these inflammatory mediators by eosinophils, after specific antigenic stimulation.
Department of Immunology, Fundación Jimenez Diaz, Madrid, Spain.
B
B
de Andres
V
V
del Pozo
E
E
Martin
P
P
Palomino
C
C
Lahoz
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D004804:Eosinophils / Q000276:immunology / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D008645:Mesocestoides / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013189:SRS-A / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*
D000947:Antigens, Helminth; D000939:Epitopes; D013189:SRS-A; D013481:Superoxides; D002118:Calcium