Agonist binding to purified glycine receptor reconstituted into giant liposomes elicits two types of chloride currents.
Using 'inside-out' membrane patches obtained from reconstituted giant liposomes containing purified glycine receptor from rat spinal cord, we have detected chloride currents elicited in response to the presence of the agonists glycine or beta-alanine. Regardless of the agonist employed, two different patterns of single channel currents could be detected, which differ in their main conductance, complexity of substates and opening frequency. In agreement with the expectations of glycine receptor heterogeneity suggested recently at the mRNA and cDNA level, our results indicate the existence of functionally different glycine receptors in the adult rat spinal cord.
Riquelme
G
G
Department of Neurochemistry, University of Alicante, San Juan, Spain.
Morato
E
E
López
E
E
Ruiz-Gómez
A
A
Ferragut
J A
JA
González Ros
J M
JM
Mayor
F
F
D000409:Alanine / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D018118:Chloride Channels; D005998:Glycine / Q000378:metabolism / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008081:Liposomes; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000502:physiology*; D051381:Rats; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D013116:Spinal Cord / Q000378:metabolism
D018118:Chloride Channels; D007473:Ion Channels; D008081:Liposomes; D008565:Membrane Proteins; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; D000409:Alanine; D005998:Glycine