A simple rapid method to slice biological specimens: an application for non-embedded and embedded Golgi-stained tissue.
A rotary wire saw is faster and simplifies markedly the slicing of soft and hard tissues without apparent loss of quality. Two applications for the Golgi-Cox method (using the rotary wire saw) are described: one eliminates specimen freezing and embedding while the other uses LR-White instead of celloidin, reducing preparation time. Sections of 20 microns to several mm thick can be obtained.
Kolodziejczyk
E
E
Section of Microscopy, Nestlé Research Centre, Nestec Ltd., Lausanne, Switzerland.
Serrant
P
P
Fernandez-Graf
M R
MR
D000818:Animals; D001921:Brain / Q000166:cytology*; D006652:Histological Techniques; D051379:Mice; D008867:Microtomy / Q000295:instrumentation / Q000379:methods*; D012834:Silver; D013194:Staining and Labeling
D012834:Silver