Oligosaccharide-related epitope specific for a brain-specific glycoprotein, 1D4 antigen.
The characteristics of glycosylation of a brain-specific glycoprotein, 1D4 antigen, and the epitope recognized by its monoclonal antibody were studied. Removal of high-mannose and hybrid types of N-linked oligosaccharides by treatment with endoglycosidase H converted the molecular mass of the 1D4 antigen from 89 kDa to 78 kDa, but did not affect its reactivity with the 1D4 monoclonal antibody. Removal of all types of N-linked oligosaccharides by treatment with glycopeptidase F or removal of both N- and O-linked oligosaccharides by chemical treatment caused both reduction of the molecular mass of the antigen to 63 kDa and loss of its reactivity with the monoclonal antibody. These results suggest that the 1D4 monoclonal antibody recognizes a complex-type oligosaccharide-related epitope specific for the 1D4 antigen. Results also showed that N-linked glycosylation was not responsible for the charge heterogeneity of the 1D4 antigen. The oligosaccharide chain-related epitope was detected in rat brain but not in mouse, rabbit, or bovine brain, but the 1D4 antigen was recognized in rat and mouse brains with antiserum (polyclonal antibodies). These findings indicate that the oligosaccharide-related epitope is species specific. Furthermore, results with neuraminidase-treated 1D4 antigen indicated that sialic acids were not involved in the oligosaccharide-related epitope. These findings suggest that the 1D4 antigen may have the oligosaccharide structure specific for rat brain and itself.
Sano
S
S
Division of Protein Metabolism, Osaka University, Japan.
Ito
S
S
Nakamura
M
M
Nakagawa
H
H
D000818:Animals; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D009844:Oligosaccharides / Q000032:analysis* / Q000276:immunology; D009928:Organ Specificity; D051381:Rats
D000939:Epitopes; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D009844:Oligosaccharides