Detection of multidrug-resistant protein, P-glycoprotein in childhood leukaemia and lymphoma.
Flow cytometric detection of surface P-glycoprotein, a multidrug-resistant gene product, with a monoclonal antibody, MRK 16, was performed on cells obtained from 18 children with leukaemia and lymphoma. Of 18 patients examined, 1 with malignant lymphoma at relapse showed a significant increase in P-glycoprotein-positive cells and a strong resistance to chemotherapy. Overexpression of P-glycoprotein in a case with B-cell type malignant lymphoma was confirmed by immuno-precipitation and Northern hybridization analysis. The present study suggests that an increased expression of surface P-glycoprotein might be involved in multidrug resistance at least in a certain case of childhood leukaemia and lymphoma.
Department of Paediatrics, Faculty of Medicine, Kyusyu University, Fukuoka City, Japan.
Y
Y
Mizuno
T
T
Hara
M
M
Nagata
A
A
Tawa
T
T
Tsuruo
K
K
Ueda
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000293:Adolescent; D000911:Antibodies, Monoclonal; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D015152:Blotting, Northern; D002648:Child; D004351:Drug Resistance; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000378:metabolism; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D011233:Precipitin Tests; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins