Impact of 5-azacytidine on insulin binding and insulin-induced receptor formation in tetrahymena.
The unicellular Tetrahymena is able to bind the vertebrate hormone insulin, and the binding sites presented by it become amplified under hormonal influence. The increased binding capacity for insulin reappears in many offspring generations. 5-azacytidine inhibits insulin binding and the insulin-induced formation of binding sites as well in the cell generation directly involved in interaction, but enhances insulin binding in the daughter cell generations. The nutrient medium of the cells whose binding capacity was enhanced by azacytidine treatment transmitted the information accounting for increased binding to "virgin" cells not previously treated with azacytidine.
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
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Csaba
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Kovács
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D011972:Receptor, Insulin / Q000187:drug effects / Q000378:metabolism*; D013768:Tetrahymena / Q000187:drug effects / Q000378:metabolism
D007328:Insulin; D011972:Receptor, Insulin; D001374:Azacitidine