[Effects of radiation on capillary endothelial cells derived from Mongolian gerbil brain].
Confluent monolayers of capillary endothelial cells derived from Mongolian gerbil brain were irradiated with a single exposure of x-rays, and their radiosensitivity and sequential changes in morphology, staining intensity for factor VIII-related antigen (F VIII RAg), and capacity to produce prostacyclin (PGI2) were examined. The radiobiologic parameters that characterized the dose-response survival curve for these cells were found to be n = 1.9, Dq = 140 rad, and D0 = 190 rad. Morphologically, nuclear and cytoplasmic swelling, vacuolation of cytoplasm, and giant cell formation occurred in a dose dependent manner after 24 hours from irradiation. Decreased staining intensity for F VIII RAg was observed in morphologically affected cells. The capacity to synthesize PGI2 was significantly enhanced at 24 hours, but less significant at 72 hours after irradiation. The present data suggest that the radiosensitivity of brain capillary endothelial cells may be somewhat lower than that of endothelial cells originated from larger vessels, and that radiation induced morphological and functional changes in the brain capillary endothelial cells may be quantitatively similar to the changes in endothelial cells of larger vessels.
Mori
S
S
Department of Neurosurgery, Niigata University, Japan.
Tanaka
R
R
Minakawa
T
T
Onda
K
K
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000528:radiation effects*; D011464:Epoprostenol / Q000096:biosynthesis; D005849:Gerbillinae; D011829:Radiation Dosage; D013194:Staining and Labeling; D014841:von Willebrand Factor
D014841:von Willebrand Factor; D011464:Epoprostenol