Preparation and characterization of neutralizing monoclonal antibodies to feline calicivirus.
Fourteen neutralizing monoclonal antibodies (N-MoAbs) were prepared against the F4 strain of feline calicivirus (FCV), the prototype strain of FCV in Japan, and examined for their ability to neutralize FCV isolates. Neutralization-resistant variants of the F4 strain were selected under the presence of 4 individual N-MoAbs in cell culture systems and used in cross-neutralization tests and enzyme-linked immunosorbent assay with all of the 14 N-MoAbs. The results revealed the identification of at least two antigenic determinants on FCV F4: one being more broadly conserved among FCV isolates than the other. Usefulness of antigenic variants resistant to N-MoAbs for analysis of neutralization determinants on FCV was also demonstrated.
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Y
Y
Tohya
Y
Y
Taniguchi
M
M
Tsubakimoto
E
E
Takahashi
T
T
Mikami
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000914:Antibodies, Viral / Q000276:immunology* / Q000378:metabolism; D002139:Caliciviridae / Q000276:immunology*; D002478:Cells, Cultured; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D009500:Neutralization Tests; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D003470:Culture Media; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M