Localization of antigenic sites in the primary sequence of the Sendai virus NP protein.
Monoclonal antibodies were used to localize five antigenic sites in the sequence of the Sendai virus NP gene. NP-specific peptides, expressed in Escherichia coli from the cloned gene served as antigens for blot analyses to determine the specificity of antibody binding. The antigenic sites were associated primarily with the C-terminal half of the protein.
Fisher
L E
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Department of Natural Sciences, University of Michigan-Dearborn MI 48128-1491.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D003001:Cloning, Molecular; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D015151:Immunoblotting; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D010455:Peptides / Q000138:chemical synthesis; D010957:Plasmids / Q000235:genetics; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D010455:Peptides; D014758:Viral Core Proteins