A heparin-binding protein from neuroblastoma cells: immunological comparison to beta-amyloid precursor protein.
1. beta-Amyloid precursor protein cross-reactive polypeptides were detected in the membrane extracts of a mouse neuroblastoma cell line, NB41A3. Four immunoreactive polypeptide bands were observed on western blots of a cell membrane extract. Their molecular weights as estimated by polyacrylamide gel electrophoresis ranged from 89.1 to 41 kDa. 2. After heparin affinity chromatography, two of these polypeptides strongly cross-reacted with an antibody that recognizes Alzheimer beta-amyloid precursor protein. 3. From the heparin binding fraction, these protein were further separated by reverse-phase high-performance liquid chromatography. A cross-reactive protein was isolated.
Department of Chemistry, University of Alaska Fairbanks 99775-0180.
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D016564:Amyloid beta-Protein Precursor / Q000032:analysis*; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D006493:Heparin / Q000378:metabolism*; D051379:Mice; D009447:Neuroblastoma / Q000737:chemistry; D014407:Tumor Cells, Cultured / Q000737:chemistry*
D016564:Amyloid beta-Protein Precursor; D002352:Carrier Proteins; D006493:Heparin