The identification and characterization of an uptake system for taurine into rat lung slices.
The objective of this study was to determine whether taurine was accumulated by rat lung slices and if so, to establish the role of this uptake as a source of pulmonary taurine. We have shown that taurine is accumulated into rat lung by an active uptake process that was both ATP and Na(+)-dependent and obeyed saturation kinetics, exhibiting an apparent Km of 186 microM and Vmax of 970 nmol/g wet wt/hr. Substrate specificity of the system was high and only compounds possessing anionic and cationic groups separated by two methylene groups were able to competitively inhibit taurine uptake. Subsequent to its uptake, taurine was not significantly metabolized, and since the apparent Km for the uptake process is similar to the known plasma concentration of taurine, it can be inferred that this system will contribute to pulmonary taurine uptake in vivo. Taurine has been suggested to possess antioxidant and antiinflammatory properties, and we suggest that this uptake system may contribute to the defence of pulmonary tissue against oxidative stress.
Biochemical Toxicology Section, Imperial Chemical Industries PLC, Macclesfield, Cheshire, U.K.
C P
CP
Lewis
G M
GM
Cohen
L L
LL
Smith
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001667:Binding, Competitive; D003994:Bucladesine / Q000494:pharmacology; D002738:Chloroquine / Q000494:pharmacology; D002746:Chlorpromazine / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D003958:Diamide / Q000494:pharmacology; D007700:Kinetics; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology; D013654:Taurine / Q000378:metabolism*
D003958:Diamide; D013654:Taurine; D005576:Colforsin; D003994:Bucladesine; D000968:Antimycin A; D002738:Chloroquine; D000255:Adenosine Triphosphate; D012964:Sodium; D011190:Potassium Cyanide; D015056:1-Methyl-3-isobutylxanthine; D002746:Chlorpromazine