[The differentiation of viruses in the Venezuelan equine encephalomyelitis complex by using monoclonal antibodies and lanthanide immunofluorescence analysis].
Potentialities of differentiation between Venezuelan equine encephalomyelitis (VEE) complex viruses by time-resolved fluoroimmunoassay and enzyme immunoassay were studied. For this, 4 test systems were used based on different combinations of native and labeled polyclonal antibodies to VEE virus, strain Trinidad, and monoclonal (MCA) antibody MAK 14-7 to protein EL of this virus. The maximal sensitivity and specificity was achieved in the test system formed from native MCA MAK 14-7 for sensitization of the solid phase and labeled polyclonal immunoglobulins for demonstration of the test results. This combination of antibodies allowed to differentiate the epidemic variant of VEF/Trinidad (IA) from epizootic variants of Mucambo (III), Pixuna (IV) and attenuated strain No. 230.
Gaĭdamovich
S Ia
SIa
Pomelova
V G
VG
Lavrova
N A
NA
Mel'nikova
E E
EE
Sokolova
M V
MV
Kharitonenkov
I G
IG
Zlobin
V N
VN
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D001202:Ascitic Fluid / Q000276:immunology; D004666:Encephalitis Virus, Venezuelan Equine / Q000145:classification* / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008674:Metals, Rare Earth; D051379:Mice
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D008674:Metals, Rare Earth
Differentsiatsiia virusov kompleksa venesuél'skogo éntsefalomielita loshadeĭ s primeneniem monoklonal'nykh antitel i lantanidnogo immunofliuorestsentnogo analiza.