title: Structural characterization of neutral oligosaccharides with blood-group A and H activity isolated from bovine submaxillary mucin.
abstract: In this study we investigated the structures of 11 neutral oligosaccharides released from bovine submaxillary mucin by alkaline borohydride treatment and isolated by h.p.l.c. One hexa-, one penta-, three tetra-, four tri- and two di-saccharides containing core types 1, 2, 3 or 4 were obtained. We report their structures, determined by a combination of one- and two-dimensional 1H n.m.r. spectroscopy at 270 MHz and methylation analysis involving g.l.c.-m.s., along with their approximate molar ratios. Only three of these oligosaccharides have previously been reported in this source. Of the new oligosaccharides, one contains the blood-group-A antigenic determinant, two contain the blood-group-H type 2 determinant, while another contains the blood-group-H type 3 determinant. The oligosaccharide GlcNAc beta (1----6)[GlcNAc beta (1----3)]GalNAcol, although previously found as a core structure, has been isolated here as a novel trisaccharide.
authors.affiliation: Department of Chemistry, University College, Galway, Ireland.
authors.forename: A V
authors.initials: AV
authors.lastname: Savage
authors.forename: S M
authors.initials: SM
authors.lastname: D'Arcy
authors.forename: C M
authors.initials: CM
authors.lastname: Donoghue
mesh_terms: D000818:Animals; D001789:Blood Group Antigens / Q000276:immunology*; D002240:Carbohydrate Sequence; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D000939:Epitopes / Q000276:immunology; D007519:Isoantigens / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009077:Mucins / Q000276:immunology*; D009844:Oligosaccharides / Q000276:immunology* / Q000302:isolation & purification; D013363:Submandibular Gland / Q000737:chemistry*
chemical_list: D001789:Blood Group Antigens; D000939:Epitopes; D007519:Isoantigens; D009077:Mucins; D009844:Oligosaccharides