Reproductive death of Chinese hamster V79 cells after exposure to chemical inhibitors of DNA synthesis.
1. The results of this study have contributed to the definition of three categories of chemical inhibitors of DNA replication in mammalian cells. 2. Inhibitors of replicon cluster initiation [4-nitroquinoline-N-oxide (4-NQO), etoposide (VP-16), teniposide (VM-26), amsacrine (m-AMSA), N-methyl-N'-nitro-N-nitrozoguanidine (MNNG), cis-Pt(II)diammine dichloride (cis-PDD)], which needed similar doses to produce a slow and persistent (up to 4 hr) inhibition of DNA synthesis, followed by significant cell killing. 3. Inhibitors of DNA replication by indirect action [3-aminobenzamide [correction of 3-aminobezamide] (3-AB), cycloheximide (CHX), puromycin (PRC), bisbenzimide Hoechst No. 33258 (H-33258]), that showed reduced cytotoxic effects, and caused a slow (60 min) and reversible inhibition of DNA synthesis. 4. Inhibitors of formation and/or polymerization of deoxyribonucleotides [5-aminouracil (5-AU), bisbenzimide Hoechst No. 33342 (H-33342)], which induced a fast (20 min) and reversible suppression of DNA replication, associated with limited cell killing.
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Department of Radiology and Radiation Biology, Colorado State University, Fort Collins 80523.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000677:Amsacrine / Q000494:pharmacology; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D006690:Bisbenzimidazole / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D003513:Cycloheximide / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects*; D005047:Etoposide / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D066298:In Vitro Techniques; D007700:Kinetics; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D013713:Teniposide / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*; D014498:Uracil / Q000031:analogs & derivatives / Q000494:pharmacology
D001549:Benzamides; D000677:Amsacrine; D008769:Methylnitronitrosoguanidine; D011691:Puromycin; D015112:4-Nitroquinoline-1-oxide; D014498:Uracil; D005047:Etoposide; C025160:3-aminobenzamide; D013713:Teniposide; D003513:Cycloheximide; D006690:Bisbenzimidazole; D002945:Cisplatin; C011166:5-aminouracil