Characterization of the carbohydrate chain on the substance P receptor in the rat brain cortex: effect of lectins on [3H]substance P binding.
The characteristics of the carbohydrate chain on the rat cerebral cortical substance P (SP) receptor were studied. We examined the effects of pretreatment with three lectins (concanavalin A, wheat germ agglutinin, lens culinaris agglutinin) on the [3H]SP binding activities. Each lectin can bind to the specific carbohydrate chain. Among these lectins, only concanavalin A inhibited specific [3H]SP binding by reducing the affinity of the binding sites. The inhibitory action of concanavalin A was dose-dependent and diminished by the addition of alpha-methyl-D-mannoside. The present results suggest that the rat cortical SP receptor has either a biantennary complex-type or a high mannose-type of carbohydrate chain, and that the carbohydrate chain is implicated in the SP binding activity of the SP receptor system.
Takamatsu
H
H
Department of Pharmacology, Hiroshima University School of Medicine, Japan.
Tani
Y
Y
Akiyama
M
M
Nakata
Y
Y
Segawa
T
T
D000818:Animals; D002240:Carbohydrate Sequence; D002540:Cerebral Cortex / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D037121:Plant Lectins; D051381:Rats; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000235:genetics* / Q000378:metabolism; D013373:Substance P / Q000378:metabolism*; D014316:Tritium; D014909:Wheat Germ Agglutinins / Q000494:pharmacology
D037102:Lectins; D037121:Plant Lectins; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D014909:Wheat Germ Agglutinins; C033619:lentil lectin; D014316:Tritium; D003208:Concanavalin A; D013373:Substance P