Some yeast proteins are recognized by antibodies to reverse transcriptases from several mammalian retroviruses and display reverse transcriptase activity.
Antibodies directed against reverse transcriptases (RT) of the mammalian retroviruses SSV and RD114 recognize specifically on Western Blots yeast cytoplasmic soluble proteins of 31 and 45 kDa respectively and inhibit RT activity in this fraction. Anti RLV RTIgG recognizes a protein of 40-43 kDa in the particulate (VLP) fraction and inhibits RT activity in it. No inhibition of RT activity was seen with normal serum anti AMV RTIgG or antisera against yeast DNA polymerase I and DNA polymerase III. These yeast RTs display RNase H activity and are not inhibited by aphidicolin. They prefer Mn+2 as cofactor over Mg+2, display an optimum temperature of 25-30 degrees C and are expressed in a diploid as well as 2 haploid strains and are thus distinct from yeast Ty encoded RTs.
Halbreich
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Laboratoire de Physiologie Cellulaire, Université Pierre et Marie Curie, Paris, France.
D000918:Antibody Specificity; D004251:DNA Transposable Elements; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology*; D007120:Immunochemistry; D012194:RNA-Directed DNA Polymerase / Q000276:immunology*; D012190:Retroviridae / Q000201:enzymology* / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000276:immunology
D004251:DNA Transposable Elements; D005656:Fungal Proteins; D012194:RNA-Directed DNA Polymerase