[A method of fractionating bone marrow cells in a density gradient using a mixture of Ficoll and Verografin].
The authors suggest a method for hemopoietic tissue cell fractionation into populations enriched for various hemopoietic elements. Centrifugation in a three-layer density gradient of mixtures of ficoll and verografin (d1 = 1.03 g/cm3, d2 = 1.09 g/cm3, d3 = 1.1 g/cm3) resulted in three fractions. The first contained lymphoid cells, the second erythroid cells (89.1 +/- 7.3%), and the third consisted of white blood cells (78.5 +/- 6.3%).
Sibirnaia
N A
NA
Sukhomlinov
B F
BF
Khmil'
M V
MV
D000818:Animals; D001854:Bone Marrow Cells; D002499:Centrifugation, Density Gradient / Q000379:methods*; D005591:Chemical Fractionation; D003974:Diatrizoate Meglumine; D005362:Ficoll; D051381:Rats
D005362:Ficoll; D003974:Diatrizoate Meglumine
Metod fraktsionirovaniia kletok kostnogo mozga v gradiente plotnosti smeseĭ fikolla i verografina.