title: Structure of the human pituitary adenylate cyclase activating polypeptide (PACAP) gene.
abstract: The human gene encoding pituitary adenylate cyclase activating polypeptide (PACAP) was isolated and its nucleotide sequence was determined. By comparison with a human PACAP cDNA, the exon/intron organization of PACAP gene was determined. The last exon encoded the longer form of PACAP, PACAP38 and 3'-untranslated sequences, suggesting that the shorter form of PACAP, PACAP27 is not generated by alternative splicing mechanisms. The 5'-flanking region of the PACAP gene contains several sequence motifs homologous to CRE, TRE, and GHF-1. On the basis of DNA isolated from mouse A9 microcell hybrid clone containing a single human chromosome, the PACAP gene was assigned to human chromosome 18. Furthermore, we determined the locus of the gene to be 18p11 by the chromosomal in situ hybridization technique.
authors.affiliation: Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd., Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hosoya
authors.forename: C
authors.initials: C
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Ogi
authors.forename: S
authors.initials: S
authors.lastname: Ohkubo
authors.forename: Y
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: H
authors.initials: H
authors.lastname: Kugoh
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Onda
authors.forename: M
authors.initials: M
authors.lastname: Oshimura
authors.forename: A
authors.initials: A
authors.lastname: Arimura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003062:Codon; D004247:DNA; D005091:Exons; D015698:Genomic Library; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011498:Protein Precursors / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid
chemical_list: C495401:ADCYAP1 protein, human; D003062:Codon; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011498:Protein Precursors; D004247:DNA