title: Enhancement of DNA transfection efficiency by heat treatment of cultured mammalian cells.
abstract: The expression of genes introduced into various mammalian cell lines was enhanced by raising the temperature of the cells to 42 degrees C for a few hours after DNA transfection. This heat treatment resulted in an up to 10-fold increase in the frequency of the cells that transiently expressed a foreign gene such as that of beta-galactosidase, whereas it had only a limited enhancing effect on the development of stable transformants. By immunotitration analysis, it was confirmed that the enhanced expression of beta-galactosidase activity correlated well with the increase of the enzyme protein. This procedure may have an applicability for augmenting the frequency of transient gene expression in many cell types.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Okayama University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takai
authors.forename: H
authors.initials: H
authors.lastname: Ohmori
mesh_terms: D000818:Animals; D002460:Cell Line; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006358:Hot Temperature; D007120:Immunochemistry / Q000379:methods; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D001616:beta-Galactosidase