RNA editing intermediates of cox2 transcripts in maize mitochondria.
Eighteen cytidines are changed to uridines in the coding sequence of transcripts for cytochrome c oxidase subunit 2 (cox2) in maize mitochondria. The temporal relationship of editing and splicing was examined in cox2 transcripts by sequence analysis of spliced and unspliced cDNAs. Cloned cDNAs of unspliced cox2 transcripts ranged from clones with no edited nucleotides to completely edited forms, while spliced cDNAs were nearly completely edited. Incompletely edited transcripts in the nascent pool of unspliced transcripts represent intermediates of the editing process. These results indicate that editing proceeds without a strong directional bias and suggest that RNA editing is a posttranscriptional process.
Yang
A J
AJ
Department of Developmental and Cell Biology, University of California, Irvine 92717.
Mulligan
R M
RM
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D005091:Exons; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics*; D000077278:RNA, Mitochondrial; D015183:Restriction Mapping; D014158:Transcription, Genetic; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
D004272:DNA, Mitochondrial; D046911:Macromolecular Substances; D000077278:RNA, Mitochondrial; D012313:RNA; D004247:DNA; D003576:Electron Transport Complex IV