Localization of common cytotoxic T lymphocyte recognition epitopes on simian papovavirus SV40 and human papovavirus JC virus T antigens.
Human papovavirus JC virus (JCV) and Simian virus 40 (SV40) tumor or T antigens demonstrate considerable sequence homology which is reflected by antibody cross-reactivity. This similarity raised the possibility that JCV and SV40 T antigen also might contain common cytotoxic T lymphocyte (CTL) recognition epitopes. In this study we identified and mapped such sites on the JCV T antigen. C57Bl/6 cell lines transformed by JCV/SV40 T antigen chimeras were generated and tested for susceptibility to lysis by five H-2b restricted SV40-specific CTL clones: Y-1, Y-2, Y-3, Y-4, and Y-5. These CTL clones recognize specific epitopes within amino acids 205-219 (site I), 220-233 (sites II and III), 369-511 (site IV), and 489-503 (site V) on SV40 T Ag, respectively. The results show that sites I, II, III, and IV (recognized by CTL clones Y-1, Y-2, Y-3, and Y-4, respectively) represent common epitopes on SV40 and JCV T antigens. CTL clone Y-5 failed to recognize JCV T antigen indicating that CTL can discriminate between the two antigenically related T antigens.
Deckhut
A M
AM
Department of Microbiology and Immunology, Pennsylvania State College of Medicine, Hershey 17033.
Tevethia
M J
MJ
Haggerty
S
S
Frisque
R J
RJ
Tevethia
S S
SS
D000595:Amino Acid Sequence; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D002461:Cell Line, Transformed; D002678:Chimera; D002999:Clone Cells / Q000382:microbiology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000302:isolation & purification*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000276:immunology*; D027382:Polyomaviridae; D013539:Simian virus 40 / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000957:Antigens, Viral, Tumor; D000939:Epitopes; D015395:Histocompatibility Antigens Class I