The rne gene is the structural gene for the processing endoribonuclease RNase E of Escherichia coli.
Using T7 RNA polymerase and specific constructs derived from 5S rRNA and RNA I genes, we generated substrates for the RNA processing enzyme RNase E. Using these substrates we have shown that a 3.2 kb DNA fragment that complements the rne-3071 mutation can express RNase E activity. We also found that T7 RNA polymerase terminates within the 5S rRNA gene.
Chauhan
A K
AK
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110.
Apirion
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D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D010957:Plasmids; D012329:RNA, Bacterial / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D014158:Transcription, Genetic
C042027:RNA I; D012329:RNA, Bacterial; D012341:RNA, Ribosomal, 5S; D004722:Endoribonucleases; C022537:ribonuclease E