Proteolipid protein and DM-20 are synthesized by Schwann cells, present in myelin membrane, but they are not fatty acylated.
Proteolipid protein (PLP) and DM-20 were intensely labeled after immunoprecipitation of total cellular proteins and myelin proteins labeled with [35S]methionine in nerve slices. These results provided evidence that PLP and DM-20 are incorporated into the myelin membrane following their synthesis in Schwann cells. In contrast, PLP and DM-20 were not fatty acylated after incubation of the nerve slices with [3H]palmitic acid, however, P0 glycoprotein and 24 kDa protein were heavily fatty acylated. The lack of fatty acylation of PLP and DM-20 in the peripheral nervous system suggests that fatty acyltransferase responsible for their acylation is absent or non-functional in the peripheral nervous system.
Agrawal
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HC
Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110.
Agrawal
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D
D000215:Acylation; D000818:Animals; D001921:Brain / Q000378:metabolism*; D066298:In Vitro Techniques; D008715:Methionine / Q000378:metabolism; D009185:Myelin Proteins / Q000096:biosynthesis*; D018991:Myelin Proteolipid Protein; D009186:Myelin Sheath / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D010525:Peripheral Nerves / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D051381:Rats; D012583:Schwann Cells / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014316:Tritium
D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; D010169:Palmitic Acids; C489071:Plp1 protein, rat; D013462:Sulfur Radioisotopes; D014316:Tritium; D019308:Palmitic Acid; D008715:Methionine