title: Localization of immunogenic domains in the human immunodeficiency virus type 2 envelope.
abstract: Highly immunogenic domains have not yet been defined in the extracellular protein of the human immunodefiency virus type 2 (HIV-2) envelope. In this study, six contiguous segments covering the entire HIV-2ST envelope were amplified and cloned into a bacterial expression vector to localize the relative immunogenic reactivity of different regions of the molecule by Western blot (immunoblot) analysis. Our results demonstrate that the extracellular protein of the HIV-2 envelope contains highly immunogenic epitopes with potential value as type-specific markers for HIV-2 infection.
authors.affiliation: Department of Cancer Biology, Harvard School of Public Health, Boston, Massachusetts 02115.
authors.forename: M L
authors.initials: ML
authors.lastname: Huang
authors.forename: M
authors.initials: M
authors.lastname: Essex
authors.forename: T H
authors.initials: TH
authors.lastname: Lee
mesh_terms: D000354:Africa, Western; D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D006800:Deltaretrovirus Infections / Q000276:immunology; D000939:Epitopes; D015686:Gene Products, env / Q000276:immunology*; D015483:HIV Antibodies / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014481:United States; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000939:Epitopes; D015686:Gene Products, env; D015483:HIV Antibodies; D015488:HIV Antigens; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins