[Diagnostic value of serum level of alpha 2-macroglobulin-trypsin complex in pancreatic diseases using a colorimetric assay with a synthetic chromogenic substrate].
We measured serum level of alpha 2-macroglobulin-trypsin complex (alpha 2M-T complex) using a colorimetric assay with a synthetic chromogenic substrate, D-gamma-tert-butyloxy-Gly-Arg-3-carboxy-4-hydroxyanilide dihydrochloride. Serum level of alpha 2M-T complex was greater in acute pancreatitis patients than in chronic pancreatitis or pancreatic cancer patients. In severe acute pancreatitis patients, both mean level and frequency of abnormal value of serum alpha 2M-T complex were significantly greater than in mild acute pancreatitis patients (13.1 +/- 12.9 vs 2.9 +/- 3.5 U/L, p less than 0.01; 100 vs 41%, p less than 0.01). In conclusion, the determination of serum level of alpha 2M-T complex can be useful for the diagnosis of severe acute pancreatitis.
Nakae
Y
Y
Second Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Hayakawa
T
T
Kondo
T
T
Shibata
T
T
Kitagawa
M
M
Sakai
Y
Y
Sobajima
H
H
Ishiguro
H
H
Tanikawa
M
M
Suzuki
T
T
D000208:Acute Disease; D000813:Anilides; D002863:Chromogenic Compounds; D004796:Clinical Enzyme Tests; D003124:Colorimetry / Q000379:methods; D006801:Humans; D009842:Oligopeptides; D010182:Pancreatic Diseases / Q000097:blood / Q000175:diagnosis*; D010195:Pancreatitis / Q000175:diagnosis; D014357:Trypsin / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000378:metabolism*
D000813:Anilides; D002863:Chromogenic Compounds; D009842:Oligopeptides; C028939:alpha-2-macroglobulin-trypsin complex; D000511:alpha-Macroglobulins; C061782:gamma-tert-butyloxy-Glu-Gly-Arg-3-carboxy-4-hydroxyanilide; D014357:Trypsin