[Hybridase cleavage of RNA. IV. Oligonucleotide probes containing 2'-deoxy-2'-fluoronucleoside and arabinofuranosylcytosine].
A synthesis of synthons which allow one to introduce 2'-deoxy-2'-fluoropyrimidine derivatives into the oligodeoxynucleotide chain by means of the standard solid phase phosphoramidite method has been developed. Oligonucleotides with 1-beta-D-arabinofuranosylcytosine were synthesized using either aC derivative with the unprotected 2'-OH group or O2,2'-anhydro-4-thiouridine. The synthesis of seven modified oligonucleotides (7 to 11 nucleotide residues) is described and their ability to form duplexes with complementary DNA have investigated as well as RNase H hydrolysis of hybrids formed by the E. coli 5S RNA and the obtained oligonucleotide probes.
Shmidt
S
S
Kuznetsova
L G
LG
Romanova
E A
EA
Nimann
A
A
Oretskaia
T S
TS
Krynetskaia
N F
NF
Metelev
V G
VG
Shabarova
Z A
ZA
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D003561:Cytarabine / Q000378:metabolism*; D003853:Deoxyribonucleosides / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012313:RNA / Q000378:metabolism*; D012341:RNA, Ribosomal, 5S / Q000378:metabolism; D016914:Ribonuclease H / Q000378:metabolism*
D003853:Deoxyribonucleosides; D009692:Nucleic Acid Heteroduplexes; D015345:Oligonucleotide Probes; D012341:RNA, Ribosomal, 5S; D003561:Cytarabine; D012313:RNA; D016914:Ribonuclease H
Gibridaznoe rasshcheplenie RNK. IV. Oligonukleotidnye zondy, soderzhashchie 2'-dezoksi-2'-ftornukleozidy i arabinofuranoziltsitozin.