title: Glutaraldehyde affects biocompatibility of bioprosthetic heart valves.
abstract: A marked release of glutaraldehyde from commercially available pericardial bioprosthetic heart valve (BHV) material in washing solutions was found by high performance liquid chromatography (up to 1.8 ppm of glutaraldehyde per gram of dry tissue). In vitro endothelial cell proliferation rate was impaired dose-dependently in the presence of increasing glutaraldehyde concentrations of the cultivation medium (r = 0.9; p less than 0.05). Cultivation of endothelial cells was impossible on the surface of commercially available BHV material, but successful and uninhibited when toxic glutaraldehyde ligands of the BHV material were antagonized by treatment with L-glutamic acid.
authors.affiliation: Second Department of Surgery, University of Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Grimm
authors.forename: E
authors.initials: E
authors.lastname: Eybl
authors.forename: M
authors.initials: M
authors.lastname: Grabenwöger
authors.forename: H
authors.initials: H
authors.lastname: Spreitzer
authors.forename: W
authors.initials: W
authors.lastname: Jäger
authors.forename: G
authors.initials: G
authors.lastname: Grimm
authors.forename: P
authors.initials: P
authors.lastname: Böck
authors.forename: M M
authors.initials: MM
authors.lastname: Müller
authors.forename: E
authors.initials: E
authors.lastname: Wolner
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001705:Bioprosthesis; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D005976:Glutaral / Q000032:analysis / Q000494:pharmacology*; D006350:Heart Valve Prosthesis; D008422:Materials Testing
chemical_list: D001672:Biocompatible Materials; D005976:Glutaral