title: Staining plant cells with silver. I. The salt-nylon technique.
abstract: A technique is described for selectively silver staining nucleoli, active nucleolus organizers, nucleolar material attached to chromosomes, kinetochores, synaptonemal complexes, and chromosome cores in plant cells. The technique, called salt-nylon silver staining, involves spreading cells on glass slides, treating the cells with a solution of saline sodium citrate, and incubating the cells in a silver nitrate solution covered with nylon screen. Selected variables important for achieving reliable silver staining are considered.
authors.affiliation: Department of Biology, Colorado State University, Fort Collins 80523.
authors.forename: S
authors.initials: S
authors.lastname: Stack
authors.forename: L
authors.initials: L
authors.lastname: Herickhoff
authors.forename: J
authors.initials: J
authors.lastname: Sherman
authors.forename: L
authors.initials: L
authors.lastname: Anderson
mesh_terms: D000490:Allium / Q000166:cytology; D000970:Antineoplastic Agents; D002466:Cell Nucleolus / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure; D008853:Microscopy; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D009757:Nylons; D059828:Plant Cells; D012834:Silver; D012835:Silver Nitrate; D013194:Staining and Labeling; D013573:Synaptonemal Complex
chemical_list: D000970:Antineoplastic Agents; D009757:Nylons; D012834:Silver; D012835:Silver Nitrate