Derivation of mixed phenotype cell lines with ras-myc- and raf-myc-containing retroviral vectors after infection of murine long-term bone marrow cultures.
Murine long-term bone marrow cultures were infected with retroviral vectors expressing the viral ras, raf, and myc oncogenes, alone and in combination. Stably transformed clonal cell lines were obtained after infection with ras-myc and raf-myc retroviruses but not by vectors expressing ras, myc, or raf alone. Northern blot analysis demonstrated that the two clonal cell lines expressed high levels of vector-specific transcripts. Phenotypic analysis of the cell lines by flow microfluorimetry and histochemical staining suggested that both cell lines expressed markers associated with cells of the megakaryocyte differentiation pathway. Histochemical staining demonstrated that these cell lines also expressed cytoplasmic enzymes associated with granulocytes and/or monocytes/macrophages. These cell lines, despite their clonal origin, are therefore of a mixed phenotype.
Spoor
E
E
Department of Experimental Hematology, Immunex Research and Development Corporation, Seattle, Washington 98101.
Ziegler
S F
SF
Overell
R W
RW
deVries
P
P
Williams
D E
DE
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D001853:Bone Marrow / Q000737:chemistry; D001854:Bone Marrow Cells; D004247:DNA / Q000032:analysis; D016259:Genes, myc / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D006651:Histocytochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009283:Naphthol AS D Esterase / Q000032:analysis; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf; D012313:RNA / Q000032:analysis; D012190:Retroviridae / Q000235:genetics*; D013194:Staining and Labeling; D014162:Transfection
D011518:Proto-Oncogene Proteins; D012313:RNA; D004247:DNA; D019908:Proto-Oncogene Proteins c-raf; D009283:Naphthol AS D Esterase; D000110:Acetylcholinesterase