Hepatocyte growth factor is a potent stimulator of human melanocyte DNA synthesis and growth.
Hepatocyte growth factor (HGF) is a potent mitogen for adult rat hepatocytes in primary culture. HGF stimulates growth and DNA synthesis of normal human epidermal melanocytes in culture. The maximal stimulation of DNA synthesis by 4.0-fold occurred with 10 ng/ml HGF. This stimulatory effect was additive with both acidic and basic fibroblast growth factors, while it was inhibited by transforming growth factor-beta 1. Melanocytes expressed a single class of specific, high-affinity receptors for HGF with a Kd of 22 pM and approximately 120 receptors/cell. Thus, HGF is a potent mitogen for normal human epidermal melanocytes.
Matsumoto
K
K
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Tajima
H
H
Nakamura
T
T
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D003857:Deoxyuridine / Q000378:metabolism; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006133:Growth Substances / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D017228:Hepatocyte Growth Factor; D006801:Humans; D007700:Kinetics; D008544:Melanocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D006133:Growth Substances; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D017228:Hepatocyte Growth Factor; D003857:Deoxyuridine