title: [The determination of phospholipase activity using the 31P-NMR spectroscopic method].
abstract: The authors have developed a rather rapid and convenient method for testing and measurement of phospholipase C (EC 3.1.4.3) activity, based on continuous recording of the signal reduction in 31P-NMR spectrum of lecithin phosphate group (chemical sigma shift = 0.2 parts per million as regards H3PO4) or of phosphocholine signal augmentation (sigma = -4 parts per million). This method permits a quantitative estimation of lecithin loss or phosphocholine accrual from the kinetics of integral intensity changes in the course of an enzymic reaction and then calculate phospholipase C activity without resorting to thin-layer chromatography traditionally used for this purpose.
authors.forename: V I
authors.initials: VI
authors.lastname: Reshetniak
authors.forename: V A
authors.initials: VA
authors.lastname: Eremin
authors.forename: S N
authors.initials: SN
authors.lastname: Dvoriantsev
authors.forename: A S
authors.initials: AS
authors.lastname: Loginov
authors.forename: G F
authors.initials: GF
authors.lastname: Rozenblat
authors.forename: E K
authors.initials: EK
authors.lastname: Ruuge
mesh_terms: D003016:Clostridium perfringens / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D010738:Type C Phospholipases
vernacular_title: Opredelenie aktivnosti fosfolipazy s metodom 31P-IaMR spektroskopii.