title: Growth of Chinese hamster ovary (CHO) cells in the presence of MMC in low-serum medium.
abstract: Chinese hamster ovary (CHO-WBLT) cells growing in McCoy's 5a with 10% fetal bovine serum (FBS) were adapted to 0.5% FBS in CHO-1 Complete Media System, a serum-free medium from Ventrex. Cells in these two media were exposed to 10(-7) M and 10(-8) M mitomycin C (MMC) for 24 h. Comparison of cell growth over 10 days showed that cells in 0.5% serum proliferate, though at a slower rate than cells in 10% serum. Treatment with MMC revealed that at 10(-7) M, MMC is cytotoxic to cells to both the media; at 10(-8) M, MMC is non-cytotoxic to cells in both media.
authors.affiliation: College of Arts and Sciences, Western Michigan University, Kalamazoo 49008.
authors.forename: K
authors.initials: K
authors.lastname: Sharma
authors.forename: D
authors.initials: D
authors.lastname: Milanowski
authors.forename: G
authors.initials: G
authors.lastname: Ficsor
mesh_terms: D000818:Animals; D016466:CHO Cells; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D003470:Culture Media; D007700:Kinetics; D016685:Mitomycin / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D016685:Mitomycin