Studies on the relationship between cell proliferation and cell death: opposite patterns of SGP-2 and ornithine decarboxylase mRNA accumulation in PHA-stimulated human lymphocytes.
To assess the relationship between cell proliferation and cell death, the mRNA accumulation of ornithine decarboxylase (ODC) and sulfated glycoprotein 2 (SGP-2) were measured in human peripheral blood lymphocytes (HPBL) 2-6 hours after stimulation with phytohemagglutinin (PHA). ODC is the rate limiting enzyme of polyamines biosynthesis and its early induction in mitogen-stimulated lymphocytes has been reported. On the other hand, SGP-2, a glycoprotein present in most mammalian tissues, is induced in classical models of apoptosis, such as dexamethasone-treated thymocytes. Indeed, a consistent amount of SGP-2 mRNA in quiescent HPBL, an early and progressive decrease of SGP-2 mRNA and a parallel increase of ODC mRNA accumulation, were observed, in PHA-stimulated HPBL, suggesting that concomitant repression of SGP-2 and induction of ODC genes contribute for the cell entering the cell cycle.
Grassilli
E
E
Istituto di Chimica Biologica, University of Modena, Italy.
Bettuzzi
S
S
Monti
D
D
Ingletti
M C
MC
Franceschi
C
C
Corti
A
A
D015152:Blotting, Northern; D002453:Cell Cycle; D002455:Cell Division; D002470:Cell Survival; D051152:Clusterin; D004587:Electrophoresis, Agar Gel; D005786:Gene Expression Regulation / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics*; D018832:Molecular Chaperones; D009693:Nucleic Acid Hybridization; D009955:Ornithine Decarboxylase / Q000235:genetics*; D010835:Phytohemagglutinins; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism*
C495242:CLU protein, human; D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D012313:RNA; D009955:Ornithine Decarboxylase