title: Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species.
abstract: The immunohistochemical reactivity of human, monkey, shrew, rat and mouse normal mammary glands was examined using methacarn-fixed paraffin-embedded specimens and acetone-fixed frozen sections using the avidinbiotin-peroxidase method for cell phenotype comparison. Actin was visualized using anti-smooth muscle actin antibody and keratin expression was determined by employing 12 different monoclonal antibodies. All these antibodies cross-reacted specifically with the species examined. Basal (myoepithelial) cells from all species showed muscle-specific actin according to reactivity with HHF35 monoclonal antibody. Keratin expression showed significant phenotypic differences among species. In human and monkey, AEL-KS2, KL1, CK8.13, AE3 and 34BE12 stained luminal cells as well as basal cells. AE1, RPN1165, CK4.62, 35BE11, M20 and RPN1162 labeled only luminal cells whereas 312C8-1 preferentially bound to basal cells. In shrews, AEL-KS2, CK8.13 and AE3 reacted to both cell types, AE1 reacted only with luminal cells, and 35BE12 and 312C8-1 selectively stained basal cells. In rodents, AEL-KS2 reacted to both cell types, CK8.13, AE3, 34BE12 and 312C8-1 stained rat basal cells, and 34BE12 and 312C8-1 reacted to mouse basal cells. The data represents cytoskeletal differences among species.
authors.affiliation: Department of Pathology, Kansai Medical University, Osaka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tsubura
authors.forename: T
authors.initials: T
authors.lastname: Hatano
authors.forename: S
authors.initials: S
authors.lastname: Hayama
authors.forename: S
authors.initials: S
authors.lastname: Morii
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal; D001940:Breast / Q000737:chemistry*; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis*; D008251:Macaca; D008321:Mammary Glands, Animal / Q000737:chemistry*; D051379:Mice; D051381:Rats; D012788:Shrews; D013045:Species Specificity
chemical_list: D000199:Actins; D000911:Antibodies, Monoclonal; D007633:Keratins