[Obtaining monoclonal antibodies against outer membrane glycoproteins of Entamoeba histolytica].
The goal of this paper was the production of monoclonal antibodies capable of detecting relevant antigens from the surface of Entamoeba histolytica trophozoites, with the purpose of using them as a diagnostic test. The cellular fusion for obtaining the monoclonal antibodies (mAb) was done with spleen cells from BALB/c mice, previously immunized with glycoproteins from the membrane, as well as Sp2/0 cells. The hybridoma supernatants were tested with ELISA, using glycoproteins and lipopeptide phosphoglycans (LPPG) as antigens. Seven hybridomas producing mAb against the glycoproteins were found. Among these, three recognize LPPG. The ability of reacting with the mAb against two molecules disappeared for all the LPPG positive ones when were treated with meta-periodate, and only three reacted against the glycoproteins. All of the mAb were of the Ig M isotypes. They were characterized by Dot blot and Western blot assays. From the results, one may deduce that some mAb recognize as epitopes the polysaccharide portion, and thus infer that they are directed of against the surface and therefore, in the future, could be used with a diagnostic purpose.
Agundis
C
C
Lab. de Inmunoquímica Unidad de Inv. biomédica, C.M.N.-IMSS, México, D.F.
Isibasi
A
A
Ortíz
V
V
Reyes
J L
JL
Paniagua
J
J
Ramírez
A
A
Kumate
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology*; D004748:Entamoeba histolytica / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010457:Peptidoglycan / Q000276:immunology*; D010743:Phospholipids / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000939:Epitopes; D008562:Membrane Glycoproteins; D010457:Peptidoglycan; D010743:Phospholipids; D015800:Protozoan Proteins; C014643:lipopeptidophosphoglycan
Obtención de anticuerpos monoclonales contra glicoproteinas de membrana externa de Entamoeba histolytica.