title: Protease nexin-1 activity in cultured Schwann cells.
abstract: We report that protease nexin-1 (PN-1), a serine protease inhibitor known to have neurite-promoting effects, is made by Schwann cells in tissue culture. Three modalities have been used to demonstrate the presence of PN-1 in Schwann cell cultures. Immunostaining of the cultures with anti-PN-1 antibody gives positive staining over cells and matrix. Western blots of Schwann cell conditioned medium (CM) using anti-PN-1 antibody show a band that co-migrates with the PN-1 standard at 45 kDa. Biochemical assay for protease inhibitory activity shows that CM inhibits thrombin activity in a calorimetric assay. The CM-mediated inhibition of thrombin is reversed if the CM is pre-incubated with anti-PN-1 antibody.
authors.affiliation: Center for Neurologic Disease, Brigham and Women's Hospital, Boston, MA 02215.
authors.forename: L P
authors.initials: LP
authors.lastname: Mulligan
authors.forename: D E
authors.initials: DE
authors.lastname: Rosenblatt
authors.forename: R
authors.initials: R
authors.lastname: Toms
authors.forename: D
authors.initials: D
authors.lastname: Johnson
mesh_terms: D016564:Amyloid beta-Protein Precursor; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D007150:Immunohistochemistry / Q000379:methods; D011480:Protease Inhibitors / Q000378:metabolism; D058255:Protease Nexins; D011956:Receptors, Cell Surface; D012583:Schwann Cells / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D016564:Amyloid beta-Protein Precursor; D002352:Carrier Proteins; D003470:Culture Media; D011480:Protease Inhibitors; D058255:Protease Nexins; D011956:Receptors, Cell Surface