title: A novel heterobifunctional linker for formyl to thiol coupling.
abstract: A maleimide hydrazide has been synthesized as a heterobifunctional cross-linking agent for thiol to formyl coupling. This linker has been applied to the coupling of the monoclonal antibody 17-1A, or an Fab' derived therefrom, to polyaldehyde dextran onto which the antineoplastic agent ellipticine has been attached. High binding avidities for the unshed antigen on the SW1116 colorectal tumor cell are retained in these drug-dextran-linker-antibody conjugates.
authors.affiliation: Department of Chemistry, Lehigh University, Bethelehem, Pennsylvania 18015.
authors.forename: N D
authors.initials: ND
authors.lastname: Heindel
authors.forename: H R
authors.initials: HR
authors.lastname: Zhao
authors.forename: R A
authors.initials: RA
authors.lastname: Egolf
authors.forename: C H
authors.initials: CH
authors.lastname: Chang
authors.forename: K J
authors.initials: KJ
authors.lastname: Schray
authors.forename: J G
authors.initials: JG
authors.lastname: Emrich
authors.forename: J P
authors.initials: JP
authors.lastname: McLaughlin
authors.forename: D V
authors.initials: DV
authors.lastname: Woo
mesh_terms: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000276:immunology*; D001547:Benzaldehydes / Q000737:chemistry; D015179:Colorectal Neoplasms / Q000276:immunology; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry; D004611:Ellipticines / Q000737:chemistry; D005561:Formates / Q000737:chemistry*; D006835:Hydrazones / Q000737:chemistry; D007140:Immunoglobulin Fab Fragments; D008300:Maleic Hydrazide / Q000737:chemistry*; D015394:Molecular Structure; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D001547:Benzaldehydes; D003432:Cross-Linking Reagents; D003911:Dextrans; D004611:Ellipticines; D005561:Formates; D006835:Hydrazones; D007140:Immunoglobulin Fab Fragments; D013438:Sulfhydryl Compounds; C069065:ellipticine-polyaldehyde dextran; C034192:ellipticine; C032175:benzaldehyde; D008300:Maleic Hydrazide