[Immunohistochemical observation on keratin filaments of cultured tumor cells by ABC staining].
Avidin-Biotin Peroxidase complex technique, ABV staining, was employed by using monoclonal anti-keratin antibody HK2 in this study. The organization and dynamics of keratins in both interphase and mitotic T56 and HeLa cells were analysed. We also observed the effects of microtubule (MT) and microfilament (MF) inhibitors, colchicine and cytochalasin B, on the organization of keratin filaments in T56 and HeLa cells. The results showed that a significant alteration in the structural organization and distribution of keratin filaments occurred during mitosis, and an extensive rearrangement of keratin networks of the two cell lines was induced in interphase after the MT and MF were disrupted by combined treatment with the two drugs, colchicine and cytochalasin B; the keratin networks turned into a star-like lattice rapidly within 1-2h. Neither colchicine nor cytochalasin B alone elicited significant organizational change in the keratin networks of the two cell lines.
Wang
J
J
Department of Biology and Cell Biology.
Yang
F
F
D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism* / Q000648:ultrastructure; D014062:Tongue Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D003571:Cytochalasin B; D007633:Keratins; D003078:Colchicine