Expression of functional receptors for interleukin-6 by human polymorphonuclear leukocytes. Downregulation by granulocyte-macrophage colony-stimulating factor.
Surface interleukin-6 receptors were identified on human polymorphonuclear leukocytes (PMNL) by monoclonal anti p80-chain antibody MT 18 Cytoplasmic RNA harvested from PMNL also contained IL-6-R transcripts. Binding of recombinant human (rh) interleukin-6 (IL-6) to IL-6-R bearing PMNL was identified by flow cytometry using phycoerythrin (PE)-conjugated ligand. Treatment of PMNL with rh granulocyte-macrophage colony-stimulating factor (GM-CSF) led to the inability of PMNL to bind MT 18 monoclonal antibody (moAb) and to display binding sites for PE-conjugated rh IL-6. Levels of IL-6-R transcripts in PMNL exposed to GM-CSF were about 5-fold below those of PMNL cultured in medium only. Though a definitive role for IL-6 to modulate the function of PMNL was not found, treatment of PMNL with rh IL-6 clearly resulted in an enhancement of transcript levels of the early response genes c-fos and c-jun in these cells, thus indicating that IL-6 binding is followed by signal transduction.
Henschler
R
R
Department of Hematology and Oncology, University of Freiburg Medical Center, Germany.
Lindemann
A
A
Brach
M A
MA
Mackensen
A
A
Mertelsmann
R H
RH
Herrmann
F
F
D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D015536:Down-Regulation; D005455:Fluorescent Antibody Technique; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010799:Phycoerythrin / Q000737:chemistry; D012313:RNA / Q000032:analysis; D011971:Receptors, Immunologic / Q000187:drug effects / Q000378:metabolism*; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D015342:DNA Probes; D015850:Interleukin-6; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins; D010799:Phycoerythrin; D012313:RNA; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D004247:DNA