Electronmicroscopic detection of the axonal coexistence of serotonin and substance P in B1-B2 raphé cells transplanted into the transected spinal cord of adult rats.
One week after a complete spinal cord transection at the thoracic (T8) level in adult rats, a suspension of rhombencephalic embryonic (day 14) cells containing the B1-B2 serotonergic groups was injected below the section. After a survival period of one month, the spinal cord was processed for an ultrastructural dual immunocytochemical detection of serotonin (5-HT) and substance P (SP). It was shown by ultrastructural dual immunolabeling that 5-HT and SP coexist in the same axon terminals of transplanted cells.
Rajaofetra
N
N
INSERM, U-336, U.S.T.L. Montpellier, France.
Kachidian
P
P
Marlier
L
L
Poulat
P
P
König
N
N
Geffard
M
M
Privat
A
A
D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D003714:Denervation; D016332:Fetal Tissue Transplantation; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009417:Nerve Tissue / Q000637:transplantation*; D011903:Raphe Nuclei / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*; D013116:Spinal Cord / Q000502:physiology* / Q000648:ultrastructure; D013373:Substance P / Q000378:metabolism*; D014018:Tissue Distribution
D012701:Serotonin; D013373:Substance P