Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood.
Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.
Schwarz
T F
TF
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilian University, Munich, Germany.
Serke
S
S
Hottenträger
B
B
von Brunn
A
A
Baurmann
H
H
Kirsch
A
A
Stolz
W
W
Huhn
D
D
Deinhardt
F
F
Roggendorf
M
M
D001769:Blood; D015139:Blotting, Southern; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D004279:DNA, Viral; D014764:Viral Proteins