Effect of culture conditions on PLP and MAG gene expression in rat glioma C6 cells.
The effects of culture conditions on the expression of myelin-specific genes, i.e. proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) in rat glioma C6 cells was studied. Early passage (40-46) cells had higher steady-state level of PLP- and MAG-specific mRNA than late (100) passage cells when grown in defined (serum-free) medium. The PLP gene expression was increased whereas the MAG gene expression was reduced in the presence of 10% fetal calf serum in either passage. The level of both PLP- and MAG-specific messages was also directly related to the cell density indicating cell contact-induced stimulation of the gene expression. Furthermore, the cells apparently secrete factors into the medium, which upregulate the gene expression in autocrine fashion. The results also indicate a dissimilarity of regulatory mechanisms involved in the expression of the PLP and MAG genes.
Kanoh
M
M
Department of Anatomy, West Virginia University School of Medicine, Morgantown.
Ye
P
P
Zhu
W
W
Wiggins
R C
RC
Konat
G
G
D000818:Animals; D003470:Culture Media; D015870:Gene Expression; D005910:Glioma / Q000378:metabolism; D009185:Myelin Proteins / Q000235:genetics*; D018991:Myelin Proteolipid Protein; D018992:Myelin-Associated Glycoprotein; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014407:Tumor Cells, Cultured
D003470:Culture Media; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D018992:Myelin-Associated Glycoprotein; D012333:RNA, Messenger