Liquid chromatographic analysis of mexiletine in serum, with alternate application to tocainide, procainamide, and N-acetylprocainamide.
A simple and precise high performance liquid chromatographic method for the determination of mexiletine in human serum or plasma is described. Following addition of N-propionylprocainamide as internal standard the specimens are extracted, under basic conditions, into methylene chloride. After removal of the aqueous layer the drug is back-extracted into dilute acid, which is then injected directly for analysis. The extraction efficiency is 79% for both mexiletine and internal standard, and the assay is linear to 4 mg/L (twice upper therapeutic concentration). Inter-run coefficients of variation are 3.0% or less. The relative retention time of mexiletine to internal standard averages 1.3. An adaptation of this method is described for an alternate application to the analysis of tocainide, procainamide and N-acetylprocainamide.
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Department of Pathology, University Hospital, Ann Arbor, MI 48109-0054.
Annesley
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D000120:Acecainide / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D008012:Lidocaine / Q000031:analogs & derivatives* / Q000097:blood; D008801:Mexiletine / Q000097:blood*; D011342:Procainamide / Q000097:blood*; D016677:Tocainide
D008801:Mexiletine; D016677:Tocainide; D000120:Acecainide; D008012:Lidocaine; D011342:Procainamide