title: N-deglycosylation and immunological identification indicates the existence of beta-subunit isoforms of the rat GABAA receptor.
abstract: beta 2- and beta 3-subunits of GABAA receptors purified from the brains of 5-10-day-old rats were investigated in the intact or completely N-deglycosylated state using the beta-subunit-specific monoclonal antibody bd-17 and polyclonal antibodies directed against synthetic amino acid sequences specific for the GABAA receptor beta 2- or beta 3-subunits. The present results seem to indicate the existence of two different isoforms of the beta 3-subunit and several different isoforms of the beta 2-subunit of the GABAA receptor which probably are produced by alternative splicing.
authors.affiliation: Department of Biochem. Psychiatry, Universitätsklinik für Psychiatrie, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Buchstaller
authors.forename: D
authors.initials: D
authors.lastname: Adamiker
authors.forename: K
authors.initials: K
authors.lastname: Fuchs
authors.forename: W
authors.initials: W
authors.lastname: Sieghart
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D001923:Brain Chemistry; D000939:Epitopes / Q000276:immunology; D006031:Glycosylation; D007163:Immunosorbent Techniques; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D051381:Rats; D011963:Receptors, GABA-A / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D046911:Macromolecular Substances; D011963:Receptors, GABA-A; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase