title: Newly established uterine cervical carcinoma cell line with co-amplification of human papillomavirus DNA and c-myc gene.
abstract: A new human tumor cell line, NCC-c-CX-1 (CX-1), was established from a uterine cervical cancer xenografted in nude mice. This cell line harbored approximately 50 to 100 copies of human papillomavirus (HPV) type 18 DNA per haploid genome, and contained about 16-fold-amplified c-myc gene with rearrangement. These genomic alterations found in CX-1 cells were also present in both primary tumor and xenografted tumor. Histopathologically, original and xenografted tumors were poorly differentiated cancer and were characterized by neuroendocrine features such as positive neuron-specific enolase and chromogranin A by immunohistochemistry and abundant neurosecretory-type granules in the cytoplasm by electron microscopy. However, the established cell line had lost the neuroendocrine features. This cervical cancer cell line may be a useful model for studying cervical carcinogenesis, especially the interaction between HPV and c-myc oncogene.
authors.affiliation: Pathology Division, National Cancer Center Research Institute, Tokyo.
authors.forename: M
authors.initials: M
authors.lastname: Gotoh
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: J
authors.initials: J
authors.lastname: Yokota
authors.forename: Y
authors.initials: Y
authors.lastname: Tsunokawa
authors.forename: M
authors.initials: M
authors.lastname: Terada
authors.forename: Y
authors.initials: Y
authors.lastname: Shimoyama
authors.forename: S
authors.initials: S
authors.lastname: Teshima
authors.forename: S
authors.initials: S
authors.lastname: Hirohashi
authors.forename: Y
authors.initials: Y
authors.lastname: Shimosato
mesh_terms: D000328:Adult; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002455:Cell Division; D002460:Cell Line; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D007700:Kinetics; D051379:Mice; D008819:Mice, Nude; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure
chemical_list: D004273:DNA, Neoplasm; D004279:DNA, Viral; D012313:RNA; D010751:Phosphopyruvate Hydratase