The antiviral effect of phorbol ester and calcium ionophore A23187 is not mediated by interferons.
Phorbol myristate acetate (PMA) and the calcium ionophore, A23187, produce a dose-dependent inhibition of vesicular stomatitis virus (VSV) replication in FL cells, with maximum inhibition when cells are pretreated with 1 ng/ml of PMA or 0.5 microM A23187. No interferon (IFN) activity was detected in culture supernatants from cells treated with these agents, and addition of anti-human IFN antibodies did not prevent the inhibition of VSV replication that they caused. However, their antiviral activities were inhibited by actinomycin D or cyclohexamide treatment. Thus, the antiviral activities of PMA and A23187 involve de novo synthesis of protein but are not mediated through the production of IFN.
Onishi
E
E
Central Virus Diagnostic Laboratory, National Institute of Health, Tokyo, Japan.
Natori
K
K
Yamazaki
S
S
D000001:Calcimycin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002460:Cell Line; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007372:Interferons / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000254:growth & development; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects*
D003470:Culture Media; D014764:Viral Proteins; D003609:Dactinomycin; D000001:Calcimycin; D007372:Interferons; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate