title: Nuclear volume and expression of T-antigen, sialosyl-Tn-antigen, and Tn-antigen in carcinoma of the human bladder. Relation to tumor recurrence and progression.
abstract: The T-antigen system and the mean nuclear volume have been proposed as risk variables in bladder tumors. This study includes 34 patients with initially noninvasive (Ta) transitional cell carcinomas who experienced different courses of disease. Tissue specimens of primary tumors were analyzed for the expression of T-antigen, Tn-antigen, and sialosyl-Tn-antigen using monoclonal antibodies (MoAb) and the lectin peanut agglutinin (PNA) in an indirect immunoperoxidase method. In addition, the mean nuclear volume was estimated by morphometry. Tissue from 7 of 13 patients (54%) who had invasive disease during a follow-up period of 5 years expressed T-antigen, as defined by MoAb HH8 in the primary tumor, whereas tissue of only 3 of 21 patients who did not have invasive disease expressed the antigen (P less than 0.02). No association was found between tumor progression to invasion and the expression of Tn-antigen or sialosyl-Tn-antigen. Tn-antigen expression was partially lost in invasive tumors (P less than 0.03) when compared with the expression in primary noninvasive tumors. A high mean nuclear volume in tissue specimens of primary tumors correlated with a progression to invasive disease (P less than 0.01). A significantly (P less than 0.003) higher mean nuclear volume was found in tumor areas that were positive for PNA compared with areas that were negative for PNA in primary tumors. A significantly lower mean nuclear volume was found in Tn-antigen-positive invasive Grade 3 tumor areas than in Tn-antigen-negative areas (P less than 0.005). The combined use of T-antigen expression and mean nuclear volume is of potential clinical interest for determining patients who are at high risk of disease progression.
authors.affiliation: Department of Experimental Clinical Oncology, Danish Cancer Society, Aarhus.
authors.forename: N C
authors.initials: NC
authors.lastname: Langkilde
authors.forename: H
authors.initials: H
authors.lastname: Wolf
authors.forename: H
authors.initials: H
authors.lastname: Clausen
authors.forename: T
authors.initials: T
authors.lastname: Kjeldsen
authors.forename: T F
authors.initials: TF
authors.lastname: Orntoft
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate; D002295:Carcinoma, Transitional Cell / Q000276:immunology* / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000473:pathology; D004187:Disaccharides / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000276:immunology / Q000648:ultrastructure; D011237:Predictive Value of Tests; D001749:Urinary Bladder Neoplasms / Q000276:immunology* / Q000648:ultrastructure*
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D004187:Disaccharides; C040286:Tn antigen; C055491:sialosyl-Tn antigen; C016467:Thomsen-Friedenreich antigen