[Polymerase and immunologic activity of reverse transcriptase of the HIV-1 virus, isolated from Escherichia coli].
The recombinant reverse transcriptase of HIV-1 virus has been isolated from Escherichia coli cells transformed by the plasmid pRT40 DNA. The 103 Kd protein produced by these cells is shown to be processed to proteins with lower molecular masses by the reverse transcriptases own protease activity as well as Escherichia coli proteases. The resulting 103-66 Kd proteins possess the polymerase activity while 51 Kd and smaller proteins are lacking the activity. The 66 and 51 Kd reverse transcriptase fragments demonstrate the positive immunological reaction with the human blood serum from the people possessing antibodies to HIV-1 virus. The recombinant reverse transcriptase of HIV-1 produced by Escherichia coli cells is shown to be useful in AIDS diagnosis in humans.
Shvetsov
Iu P
IuP
Mel'nikov
A A
AA
Suvorova
Z K
ZK
Kopylova-Sviridova
T N
TN
Pokrovskiĭ
V V
VV
Fodor
I I
II
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis / Q000201:enzymology; D015153:Blotting, Western; D002848:Chromatography, DEAE-Cellulose; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D015488:HIV Antigens / Q000276:immunology*; D015497:HIV-1 / Q000201:enzymology*; D006801:Humans; D010957:Plasmids; D012194:RNA-Directed DNA Polymerase / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism
D015488:HIV Antigens; D011994:Recombinant Proteins; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase
Polimeraznaia i immunologicheskaia aktivnost' rekombinantnoĭ obratnoĭ transkriptazy virusa HIV-1, vydelennoĭ iz Escherichia coli.