title: Molecular cloning and sequence analysis of the murine cDNA for the cystic fibrosis transmembrane conductance regulator.
abstract: We have cloned the mouse homolog of the human cystic fibrosis transmembrane conductance regulator (CFTR) using clones isolated from a mouse lung cDNA library and using amplification of cDNA to isolate specific regions. The cDNA was 6304 bp in length and encoded a polypeptide of 1476 amino acids. Comparison of the deduced amino acid sequence showed that the mouse protein has high homology to the human protein; overall identity was 78.3%. The amino acid identity was high for both transmembrane domains (first transmembrane domain, 86.7%; second transmembrane domain, 81.1%) and for both ATP-binding folds (first ATP-binding fold, 80.5%; second ATP-binding fold, 83.9%), suggesting the functional importance of these regions. On the other hand, the R domain was less well conserved (68.9% identity). All of the published missense mutation sites and the site of the common delta F508 mutation were conserved between human and mouse.
authors.affiliation: Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: T
authors.initials: T
authors.lastname: Yorifuji
authors.forename: W K
authors.initials: WK
authors.lastname: Lemna
authors.forename: C F
authors.initials: CF
authors.lastname: Ballard
authors.forename: C L
authors.initials: CL
authors.lastname: Rosenbloom
authors.forename: R
authors.initials: R
authors.lastname: Rozmahel
authors.forename: N
authors.initials: N
authors.lastname: Plavsic
authors.forename: L C
authors.initials: LC
authors.lastname: Tsui
authors.forename: A L
authors.initials: AL
authors.lastname: Beaudet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D004247:DNA / Q000235:genetics; D005796:Genes; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: C505032:CFTR protein, human; D008565:Membrane Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D004247:DNA