title: Characterisation of the osteoblastic phenotype by the use of differential hybridization.
abstract: To identify genes active in cells of the osteoblast lineage we have begun to characterise the phenotype of the rat osteoblast-like osteosarcoma cell line ROS 17/2.8. We have used the method of differential hybridization to identify cDNA clones encoding mRNA species which are expressed in ROS 17/2.8 cells but not in a non-osteoblast-like osteosarcoma cell line (ROS 25/1). We have identified a number of gene products exhibiting this pattern of expression.
authors.affiliation: Institut für Pathologie, GSF, Müchen.
authors.forename: B
authors.initials: B
authors.lastname: Lanske
authors.forename: R D
authors.initials: RD
authors.lastname: Hesch
authors.forename: H M
authors.initials: HM
authors.lastname: Kronenberg
authors.forename: M J
authors.initials: MJ
authors.lastname: Atkinson
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D010006:Osteoblasts / Q000166:cytology*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012513:Sarcoma, Experimental / Q000235:genetics* / Q000473:pathology
chemical_list: D012333:RNA, Messenger