Partial purification of human prostatic 5 alpha-reductase (3-oxo-5 alpha-steroid:NADP+ 4-ene-oxido-reductase; EC 1.3.1.22) in a stable and active form.
Human hyperplastic prostate tissue was homogenised in high ionic strength buffer and the post nuclear homogenate was incubated with 0.8% octyl glucoside and bovine brain lipids. Dialysis of the resulting liposome suspension yielded a preparation in which 5 alpha-reductase was active and stable for at least three weeks and showed an increase in specific activity (Vmax +/- SD = 48.9 +/- 7.4 pmol DHT/mg protein/ml) over that of the starting homogenate (Vmax +/- SD = 5.6 +/- 1.5 pmol DHT/mg protein/min) of 8.7 times.
Sargent
N S
NS
University Department of Surgery (WGH), Western General Hospital, Edinburgh, Scotland.
Habib
F K
FK
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D003902:Detergents / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008055:Lipids; D008297:Male; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000201:enzymology; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000201:enzymology; D011480:Protease Inhibitors / Q000494:pharmacology
D003902:Detergents; D008055:Lipids; D011480:Protease Inhibitors; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase