title: Characterization of a human trypsin resistant to Kunitz soybean trypsin inhibitor. Studies of duodenal juices after tube instillation of raw soybean extract.
abstract: Human duodenal juices collected during tube instillation of raw soybean extract into the duodenum contained free trypsin and free Kuntiz soybean trypsin inhibitor (KTI) in the simultaneous presence of trypsin-KTI complexes. It has previously been suggested that this KTI-non-inhibitable trypsin has a general resistance to serine protease inhibitors. Four different trypsin forms have been found and partly characterized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and isoelectric focusing followed by Western immunoblotting or enzyme staining. In addition, crossed immunoelectrophoresis and affinity chromatography with antibody-coupled gels have been used for identification of free and inhibitor-complexed trypsin.
authors.affiliation: Matforsk, Norwegian Food Research Institute, Aas.
authors.forename: L I
authors.initials: LI
authors.lastname: Thorsen
authors.forename: H
authors.initials: H
authors.lastname: Holm
authors.forename: J E
authors.initials: JE
authors.lastname: Reseland
authors.forename: S
authors.initials: S
authors.lastname: Bjørnsen
authors.forename: L E
authors.initials: LE
authors.lastname: Hanssen
authors.forename: F
authors.initials: F
authors.lastname: Brosstad
mesh_terms: D015153:Blotting, Western; D002846:Chromatography, Affinity; D004386:Duodenum / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007419:Intestinal Secretions / Q000737:chemistry*; D007525:Isoelectric Focusing; D013025:Glycine max; D013194:Staining and Labeling; D014357:Trypsin / Q000032:analysis* / Q000145:classification; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000032:analysis*
chemical_list: D014360:Trypsin Inhibitor, Kunitz Soybean; D014357:Trypsin