Quality of oocytes from superovulated rhesus monkeys.
The parameters, serum oestradiol (E2) response, follicle size and cumulus morphology, which are commonly used to determine in-vivo oocyte maturation in human in-vitro fertilization and embryo transfer (IVF-ET) programmes, were shown to be unreliable predictors of maturation of rhesus oocytes. In two groups of rhesus, one stimulated with pregnant mares' serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) and the other with human menopausal gonadotrophin (HMG) and HCG, the three parameters varied widely within and between protocols. Triple fluorochrome staining (TFS) for chromatin, microtubules and filamentous actin (f-actin) in oocytes at the time of collection and following 24 h in culture showed major differences in their maturation both in vivo and in vitro following priming with PMSG and HMG. In evaluating IVF protocols, TFS provides a valuable assay for the meiotic status of fixed oocytes of non-human primates.
Johnson
L D
LD
New England Regional Primate Research Center (NERPRC), Harvard Medical School, Southborough, MA 01772.
Mattson
B A
BA
Albertini
D F
DF
Sehgal
P K
PK
Becker
R A
RA
Avis
J
J
Biggers
J D
JD
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004624:Embryo Transfer; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D008253:Macaca mulatta; D008297:Male; D008540:Meiosis / Q000187:drug effects; D008596:Menotropins / Q000494:pharmacology*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D010062:Ovulation Induction; D010312:Parthenogenesis; D008941:Spindle Apparatus / Q000187:drug effects; D013194:Staining and Labeling; D013480:Superovulation
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D004958:Estradiol; D008596:Menotropins