Rapid serotyping of infectious pancreatic necrosis virus by one-step enzyme-linked immunosorbent assay using monoclonal antibodies.
A one-step ELISA has been developed for detection and serotyping of infectious pancreatic necrosis virus (IPNV) in infected cell cultures using monoclonal antibodies (mAb) raised against strains representing the Sp, Ab and VR 299 serotypes of IPNV. This assay uses a serotype-specific mAb as capture and a mAb directed against a common epitope as detector antibody. Avidin-peroxidase was employed for amplification. The assay was performed in 90 min by simultaneous incubation of the samples, the biotin labelled mAb and the avidin-peroxidase, and detected 37 ng/ml of purified virus. Serotyping of 34 isolates from different areas of Europe, Asia and America showed a total concordance with the results obtained by the neutralization assay.
Domínguez
J
J
INIA, Dpto. de Sanidad Animal, Madrid, Spain.
Babín
M
M
Sánchez
C
C
Hedrick
R P
RP
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes / Q000032:analysis; D005393:Fish Diseases / Q000382:microbiology; D005399:Fishes; D012087:Reoviridae / Q000145:classification* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods / Q000662:veterinary
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D001710:Biotin