Erythrocyte membranes alteration in a shear stress measured by fluorescence anisotropy.
An experimental setup has been designed to allow fluorescence anisotropy measurements on labeled cell membranes under shear stress. An important change is observed when increasing the shear stress and varying the experimental parameters indicates that a decrease in membrane cohesion leads to a subsequent increase in the membrane alteration under shear stress. A model has been developed that shows, in agreement with experiment, that the effect observed is mainly the result of the alteration of the membrane, elongation, and orientation with respect to the fluid flow, which can be estimated.
Bouchy
M
M
LA 328 du CNRS, Nancy, France.
Donner
M
M
Andre
J C
JC
D002784:Cholesterol / Q000494:pharmacology; D002788:Cholesterol Esters / Q000494:pharmacology; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000502:physiology*; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006801:Humans; D008954:Models, Biological; D013314:Stress, Mechanical; D013696:Temperature
D002788:Cholesterol Esters; D005456:Fluorescent Dyes; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; D002784:Cholesterol; C013440:cholesteryl succinate