[Bioluminescent determination of NAD- and NADP-dependent lymphocytic glutamate dehydrogenases].
A sensitive bioluminescent rapid method for estimating the activity of pyridine nucleotide-(phosphate)-dependent glutamate dehydrogenases (NAD(P)-GDH) of human peripheral blood lymphocytes is suggested. 10000 cells is sufficient per analysis, up to 100 measurements may be made within 3 hrs. Normal subjects and subjects often suffering from acute respiratory diseases were examined for the aforesaid parameter. The findings evidence the diagnostic significance of NAD(P)-GDH for the detection of subjects at risk of developing acute respiratory diseases.
Savchenko
A A
AA
D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000201:enzymology*; D008722:Methods; D012120:Respiration Disorders / Q000201:enzymology
D005969:Glutamate Dehydrogenase; C061421:glutamate dehydrogenase (NAD(P)+)
Bioliuminestsentnoe opredelenie aktivnosti NAD- i NADF-zavisimykh glutamatdegidrogenas limoftsitov.