Spectrofluorometric determination of lipase activity.
A fluorescent triglyceride, 1(3)-pyrenylbutanoyl-2,3(1,2)-dipalmitoyl-sn-glycerol, was synthesised, characterised by NMR and mass spectrometry, incorporated into a lipid emulsion and used as a fluorescent substrate for pancreatic lipase. It is shown that the product of the reaction, pyrene butyric acid, diffuses into the aqueous phase resulting in a decrease in the excimer fluorescence of the pyrene fluorophore in the emulsion and an increase in its monomer fluorescence. The phenomenon can be used to assay the enzyme thereby cirumventing the need to extract the fatty acid product.
Liodakis
A
A
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Australia 3052.
Drew
J
J
Chan
R Y
RY
Sawyer
W H
WH
D005456:Fluorescent Dyes; D006801:Humans; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010179:Pancreas / Q000201:enzymology*; D011721:Pyrenes / Q000032:analysis / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D014280:Triglycerides / Q000378:metabolism*
D005456:Fluorescent Dyes; D011721:Pyrenes; D014280:Triglycerides; C070345:1(3)-pyrenebutanoyl-2,3(1,2)-dipalmitoyl-sn-glycerol; C002218:1-pyrenebutyrate; D008049:Lipase