title: Induction of morphological change by tyrosine kinase inhibitors in Rous sarcoma virus-transformed rat kidney cells.
abstract: Erbstatin and methyl 2,5-dihydroxycinnamate, related tyrosine kinase inhibitors, induced a morphological change in temperature-sensitive Rous sarcoma virus-transformed rat kidney (RSVts-NRK) that brought the cells close to the morphology of their normal counterpart. Erbstatin did not change the morphology of normal or Kirsten sarcoma virus-transformed rat kidney cells. Erbstatin also inhibited morphological transformation of RSVts-NRK cells induced by a shifting in temperature. Actin stress fibres were observed only in normal cells and not in transformed cells. Erbstatin induced stress fibre organization in transformed cells. Erbstatin and methyl 2,5-dihydroxycinnamate increased fibronectin gene expression in RSV-transformed cells. Thus, tyrosine kinase inhibitors induced normal phenotypes specifically in v-src-expressing cells.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Umezawa
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Hori
authors.forename: S
authors.initials: S
authors.lastname: Abe
authors.forename: R
authors.initials: R
authors.lastname: Sekizawa
authors.forename: M
authors.initials: M
authors.lastname: Imoto
mesh_terms: D000199:Actins / Q000378:metabolism; D001358:Avian Sarcoma Viruses; D015152:Blotting, Northern; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002934:Cinnamates / Q000494:pharmacology*; D005353:Fibronectins / Q000235:genetics; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation; D006873:Hydroquinones / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D012313:RNA / Q000032:analysis
chemical_list: D000199:Actins; D002934:Cinnamates; D005353:Fibronectins; D005456:Fluorescent Dyes; D006873:Hydroquinones; C067472:methyl 2,5-dihydroxycinnamate; D012313:RNA; D011505:Protein-Tyrosine Kinases; C048212:erbstatin