Neonatal complement depletion results in predominant expression of a myeloma M 104E private idiotope among anti-dextran antibodies.
Myeloma M 104E (IgM, lambda 1) with specificity for the alpha(1----3) glucosidic linkage of dextran B 1355 S (Dex) carries two idiotopes (Id) as defined by isogenic anti-idiotype mAb. The public Id is not influenced by two amino acid replacements in CDR 2 nor by an alternative D region sequence. It is shared by all or nearly all humoral antibodies in the primary immune response against Dex of mice carrying haplotype Igha. The private Id-5' appears to depend on the integrity of the VH germ-line sequence and on the particular M 104E D region sequence. It is present on a highly fluctuating but usually small fraction of primary anti-Dex antibody. We report here that this situation is changed when mice are treated with Cobra venom anti-complement factor (CVF), after birth and thereby were deprived of complement for the first two weeks of life. When immunized with Dex as adults the majority of anti-Dex Ab carried the M 104E Id-5. Thus, humoral antibody in CVF-treated animals resembled the Ly-1+ anti-Dex precursor B cell population in the peritoneal cavity, while anti-Dex Ab in animals not treated with CVF more closely corresponded to the Ly-1- precursor B cell pool in spleen (H.-P. Lehmann and G. Lehle, Eur. J. Immunol. 1991. 21: 1201).
Weiler
E
E
Fakultät für Biologie, Universität Konstanz, FRG.
Lehle
G
G
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000950:Antigens, Ly / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D003165:Complement System Proteins / Q000502:physiology*; D003911:Dextrans / Q000276:immunology*; D004546:Elapid Venoms / Q000494:pharmacology; D000939:Epitopes / Q000032:analysis*; D005803:Genes, Immunoglobulin; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000276:immunology*
D000950:Antigens, Ly; D003911:Dextrans; D004546:Elapid Venoms; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D009194:Myeloma Proteins; C016310:cobra venom factor; D003165:Complement System Proteins