[Proliferation-associated antigens PCNA and Ki-67 in two- and three-dimensional experimental systems of human squamous epithelial carcinomas].
Multiparameter-flow-cytometry was used to compare the levels of two proliferation-associated antigens, proliferating cell nuclear antigen (PCNA) and the Ki-67-related antigen, in squamous carcinoma cells grown as monolayers, multicellular spheroids (MCTS), and xenograft tumors. While the level of Ki-67-positive cells decreased with time of culture, the percentage of PCNA-positive cells stayed high in all experimental states investigated (up to 5 weeks of growth). The reduction of the mean fluorescence of PCNA/cell indicated a different way of regulation of this antigen in squamous carcinoma cells in comparison to other cell types.
Knüchel
R
R
Institut für Pathologie, Universität Regensburg.
Hofstaedter
F
F
Sutherland
R M
RM
Keng
P C
PC
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D019394:Ki-67 Antigen; D009687:Nuclear Proteins / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen; D014183:Transplantation, Heterologous
D000951:Antigens, Neoplasm; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen
Die proliferationsassoziierten Antigene PCNA und Ki-67 in zwei- und dreidimensionalen experimentellen Systemen des humanen Plattenepithelkarzinoms.