Universal antibodies to human interferon-alpha subtypes--the production of antipeptide antibodies to conserved regions of interferon-alpha.
Antibodies to three conserved regions of all human interferon (IFN)-alpha 1 subtypes were raised by immunizing rabbits with short synthetic peptides coupled to a carrier. These peptides correspond to amino acid residues 29-36, 31-36, 126-131, 139-151, and 142-151 of the consensus sequence of IFN-alpha 1. The antibodies were tested for reactivity with IFN-alpha 1, -alpha 2a, -alpha 2b, -alpha 4a, and -alpha 14 and IFN-beta. Most antipeptide antibodies react only weakly with IFN-alpha subtypes; only the antibodies raised against the peptide corresponding to residues 142-151 conjugated to keyhole limpet hemocyanin using glutaraldehyde react appreciably with all IFN-alpha subtypes tested. These antipeptide antibodies are potentially universal antibodies to the human IFN-alpha 1 subtypes, since they exhibit similar binding affinity to all the IFN-alpha subtypes tested and do not react with IFN-beta.
Sattayasai
N
N
Department of Biochemistry, Monash University, Clayton Victoria, Australia.
McMullen
G L
GL
Marzuki
S
S
Tribbick
G
G
Geysen
H M
HM
Tymms
M J
MJ
Linnane
A W
AW
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000276:immunology; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005976:Glutaral; D006801:Humans; D007202:Indicators and Reagents; D007370:Interferon Type I / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A / Q000276:immunology; D013388:Succinimides
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D000939:Epitopes; D007202:Indicators and Reagents; D007370:Interferon Type I; D010446:Peptide Fragments; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A; D013388:Succinimides; C013097:3-maleimidobenzoyl N-hydroxysuccinimide; D005976:Glutaral