title: An O antigen can interfere with the function of the Yersinia pseudotuberculosis invasin protein.
abstract: Escherichia coli strains harbouring the Yersinia pseudotuberculosis inv gene are able to enter cultured mammalial cells. We show here that this property is not shared by all enteric bacteria, since Shigella flexneri 2a cured of its virulence-associated plasmid and harbouring the inv gene is unable to enter mammalian cells efficiently. Mapping studies showed that the region of the chromosome responsible for this phenotype includes rfaB, a locus involved in the production of O antigen. S. flexneri 2a strains that express O antigen were unable to enter mammalian cells, even though invasin was efficiently expressed and localized, showing that this structure interferes with invasin activity. The O antigen either masks invasin or sterically hinders the ability of the mammalian cell receptor to bind this protein.
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111.
authors.forename: D L
authors.initials: DL
authors.lastname: Voorhis
authors.forename: S
authors.initials: S
authors.lastname: Dillon
authors.forename: S B
authors.initials: SB
authors.lastname: Formal
authors.forename: R R
authors.initials: RR
authors.lastname: Isberg
mesh_terms: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D006801:Humans; D019081:O Antigens; D010641:Phenotype; D012763:Shigella flexneri / Q000235:genetics* / Q000472:pathogenicity; D014407:Tumor Cells, Cultured; D014774:Virulence / Q000235:genetics; D015011:Yersinia pseudotuberculosis / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D019081:O Antigens; C053476:invasin, Yersinia