The plasmid-encoded arsenical resistance pump: an anion-translocating ATPase.
An anion-translocating ATPase has been identified as the product of the arsenical resistance operon of resistance plasmid R773. When expressed in Escherichia coli this ATP-driven oxyanion pump catalyses extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents. The pump is composed of two polypeptides, the products of the arsA and arsB genes. This two-subunit enzyme produces resistance to arsenite and antimonite. A third gene, arsC, expands the substrate specificity to allow for arsenate pumping and resistance.
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Department of Biochemistry, Wayne State University, School of Medicine, Detroit, MI 48201.
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001151:Arsenic / Q000378:metabolism*; D053501:Arsenite Transporting ATPases; D018053:Arsenites; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D016623:Ion Pumps; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D009586:Nitrogen Fixation / Q000235:genetics; D009876:Operon; D012689:Sequence Homology, Nucleic Acid
D018053:Arsenites; D001426:Bacterial Proteins; D007473:Ion Channels; D016623:Ion Pumps; D009097:Multienzyme Complexes; D000251:Adenosine Triphosphatases; D053501:Arsenite Transporting ATPases; C015001:arsenite; D001151:Arsenic