Structure of the gene for the neuronal intermediate filament protein alpha-internexin and functional analysis of its promoter.
We have isolated the gene encoding the neuronal intermediate filament protein alpha-internexin using low stringency hybridization conditions and an NF-M (neurofilament middle molecular weight subunit) cDNA probe. This gene consists of three exons and two introns. The sequence data and the exon-intron organization of the gene establish its classification as a type IV intermediate filament gene. Transient transfection experiments showed that up to 5 kilobases of the alpha-internexin promoter region transcribed equally well in both the alpha-internexin-expressing and -nonexpressing cell lines. The results also demonstrated that the region from -77 to +73 relative to the transcription start site was sufficient for accurate basal transcription, but inclusion of the -254 to -78 region was required for efficient transcription. Sequence analysis shows that the -254 to -78 region contains several potential positive regulatory elements.
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Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Liem
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D004247:DNA / Q000235:genetics; D005091:Exons; D005796:Genes; D007381:Intermediate Filament Proteins / Q000235:genetics*; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis; D051381:Rats; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection
D002352:Carrier Proteins; D007381:Intermediate Filament Proteins; C046643:alpha-internexin; D012313:RNA; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase