[The efficacy of immunoenzyme assay in determining IgG in blood].
The efficacies of two methods for measuring human blood serum IgG, heterogenic enzyme immunoassay (EIA) and radial immunodiffusion in gel (RIG), are compared. The accuracy of both the methods was verified by the data of IgG spectrophotometry; IgG were isolated by ion exchange chromatography from human blood serum. The results of all the three methods were in high correlation: correlation coefficient of spectrophotometry and EIA data was 0.992, p less than 0.01; that of spectrophotometry and RIG data 0.975, p less than 0.01; that of RIG and EIA 0.888, p less than 0.01. Since EIA has some advantages over RIG (it is more rapid, sensitive, accurate, and the investigation may be automated), it is recommended for measuring human blood serum IgG in mass screenings.
Iurov
S V
SV
Pchelintsev
S Iu
SIu
Cherenkova
G V
GV
D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*
D007074:Immunoglobulin G
Effektivnost' immunofermentnogo analiza dlia opredeleniia IgG v syvorotke krovi.