title: Immunological relevance of malonic dialdehyde (MDA): IV. Further evidences about the epitope recognized by antibodies obtained from rabbits immunized with MDA-modified lysozyme.
abstract: Reactions of MDA with primary amino groups produce inter- or intra-molecular 1-amino-3-imino-propene (AIP) bridges, leading to structural modifications of biological molecules. In this work, applying electrophoresis followed by transfer onto nitrocellulose membranes, we observed that serum of a rabbit immunized with MDA-modified lysozyme (ML) reacts not only with ML and native lysozyme (L), but also with MDA-modified ribonuclease, cytochrome c or polylysine (MR, MC and MP respectively), while it does not react with native ribonuclease, cytochrome c or polylysine (R, C and P respectively). These results confirm previous ones indicating that sera of rabbits immunized with ML contain antibodies reacting specifically with epitopes containing AIP bridges.
authors.affiliation: Centre de Recherches du Service de Santé des Armées, La Tronche, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Chancerelle
authors.forename: C
authors.initials: C
authors.lastname: Alban
authors.forename: R
authors.initials: R
authors.lastname: Viret
authors.forename: F
authors.initials: F
authors.lastname: Tosetti
authors.forename: J F
authors.initials: JF
authors.lastname: Kergonou
mesh_terms: D000818:Animals; D000906:Antibodies; D000939:Epitopes; D007114:Immunization; D007120:Immunochemistry; D008315:Malondialdehyde / Q000276:immunology*; D009113:Muramidase / Q000276:immunology; D011817:Rabbits
chemical_list: D000906:Antibodies; D000939:Epitopes; D008315:Malondialdehyde; D009113:Muramidase