Colony-forming efficiency response of bone marrow stromal cells to acute blood loss.
Colony-forming efficiency (CFE) was used to monitor the proliferative response of alkaline phosphatase-positive rabbit bone marrow stromal cells to acute blood loss. The CFE of animals subjected to a 1% blood loss was 0.97 compared with 0.06 (p less than 0.01) in nonbled animals. Sera obtained from animals 10 days after an initial blood loss stimulated the CFE of marrow cultures from nonbled donors to the same degree as osteogenin. Erythropoietin and control sera (from nonbled animals) had no effect. Hence, acute blood loss and sera from bled animals stimulate proliferation of alkaline phosphatase-positive marrow stromal cell colonies. The agent(s) responsible is unknown but it is present in serum in response to blood loss. Confirmation of a specific effect on osteoprogenitor cells may warrant the designation "osteopoietin."
Lippiello
L
L
Department of Orthopaedic Surgery, University of Nebraska Medical Center, Omaha 68198.
Chavda
D
D
Connolly
J
J
D000818:Animals; D001769:Blood; D001853:Bone Marrow / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004921:Erythropoietin / Q000494:pharmacology; D006470:Hemorrhage / Q000473:pathology*; D011817:Rabbits; D013234:Stem Cells / Q000502:physiology*
D004921:Erythropoietin