Detection of human immunodeficiency virus type 1 genomic RNA in plasma samples by reverse-transcription polymerase chain reaction.
An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficiency virus type 1 (HIV-1) viremia is described. The amplification of specific HIV-1 sequences of gag and env viral genes was carried out after the reverse-transcription of plasma samples (plasma RT-PCR) from seropositive subjects. The assay is faster and cheaper than detection of specific HIV-1 transcripts from peripheral blood mononuclear cells by RT-PCR. The data suggest that HIV-1 viremia is detectable by plasma RT-PCR in a large proportion of seropositive individuals.
Bagnarelli
P
P
Institute of Microbiology, University of Ancona Medical School, Italy.
Menzo
S
S
Manzin
A
A
Giacca
M
M
Varaldo
P E
PE
Clementi
M
M
D015752:Genes, env / Q000235:genetics; D015750:Genes, gag / Q000235:genetics; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis*; D006679:HIV Seropositivity / Q000097:blood; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses / Q000235:genetics; D012367:RNA, Viral / Q000097:blood*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014766:Viremia / Q000175:diagnosis*
D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase