A mammalian sperm lectin related to rat hepatocyte lectin-2/3. Purification from rabbit testis and identification as a zona binding protein.
In rat liver the asialoglycoprotein receptor is composed of three polypeptides, RHL-1, RHL-2 and RHL-3. In rat testis and spermatozoa a galactosyl receptor (RTG-r) which is immunologically related to RHL-2/3 has been described. We now report that in addition to its presence in the rat, an antigenic species of 54 kDa related to RHL-2/3 is present on rabbit, human, pig and mouse spermatozoa. Purified rabbit testis galactosyl receptor (RbTG-r) consists of two major proteins of 54 and 49 kDa, while purified rabbit liver galactose lectin consists of two major proteins of 43 and 40 kDa. In an ELISA the purified rabbit testis galactosyl receptor was shown to bind biotinylated heat solubilized rabbit zonae, while the purified liver galactose lectin did not. We conclude that one of the mammalian sperm's zona binding proteins is a galactose lectin of 54 kDa related to rat liver RHL-2/3.
Abdullah
M
M
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill.
Widgren
E E
EE
O'Rand
M G
MG
D000818:Animals; D037263:Asialoglycoprotein Receptor; D015153:Blotting, Western; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D011817:Rabbits; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D011971:Receptors, Immunologic / Q000737:chemistry / Q000276:immunology*; D013094:Spermatozoa / Q000737:chemistry* / Q000276:immunology; D013194:Staining and Labeling; D013737:Testis / Q000737:chemistry* / Q000276:immunology; D015044:Zona Pellucida / Q000378:metabolism*
D037263:Asialoglycoprotein Receptor; D037102:Lectins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C039707:galactose receptor