[An immunoenzyme method of detecting Vibrio cholerae cytolysin].
Enzyme immunoassay was used in detection of V. cholerae cytolysin. Conjugate of immunoglobulins to purified cytolysin with horseradish peroxidase was used, obtained by the periodate technique. The method sensitivity is 2 ng/ml of purified cytolysin. The results of hemolytic activity measurements in supernatants of 40 V. cholerae strains and enzyme immunoassay findings were in high correlation.
Babitsyn
S N
SN
Tsittser
A O
AO
Semiotrochev
V L
VL
Sedina
S G
SG
D003603:Cytotoxins / Q000032:analysis*; D007124:Immunoenzyme Techniques; D014734:Vibrio cholerae / Q000032:analysis*
D003603:Cytotoxins
Immunofermentnyĭ metod indikatsii tsitolizina Vibrio cholerae.