Trace expression of the germ-cell alkaline phosphatase gene in human placenta.
We have developed hybridization probes that clearly distinguish the RNA transcripts of the two closely related human heat-stable alkaline phosphatase genes. RNA from the PLAP-1 gene, encoding the term placental alkaline phosphatase, is the predominant transcript in placenta from 8 weeks to term. Transcripts from the PLAP-2 gene, encoding the germ-cell or PLAP-like enzyme, are also detectable in the placenta, but at no more than 2 per cent the level of PLAP-1 transcripts.
Povinelli
C M
CM
Department of Pathology, University of Texas Health Sciences Center, Houston 77225.
Knoll
B J
BJ
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000378:metabolism*; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D015347:RNA Probes; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic
D007527:Isoenzymes; D015347:RNA Probes; D012313:RNA; D000469:Alkaline Phosphatase