[Influence of the active compounds isolated from pilose antler on syntheses of protein and RNA in mouse liver].
The polyamines of pilose antler (PASPA) consist of putrescine (PU, 70.9%), spermidine (SPD, 26.3%) and spermine (SP, 2.8%). The incorporations of [3H] leucine into protein and [3H] uridine into RNA in mouse liver tissue were increased when PASPA was given orally to mice at the dose of 30 mg/kg for 4 successive days. The incorporations of [3H] leucine into liver protein and [3H] uridine into the cytosolic and nuclear RNA were also increased by treatment with PU (21 mg/kg). In addition, the RNA polymerase activity in the solubilized liver nuclear fraction of PU (21 mg/kg)-treated mice was increased. SPD only promoted the synthesis of protein in mouse liver tissue at the dose of 8 mg/kg. However, SP showed no effect on the synthesis of protein and RNA polymerase activity under the used dose (1 mg/kg). The results suggest that PASPA is the main active substance responsible for the promotion of the synthesis of protein and RNA in mouse liver.
Wang
B X
BX
Department of Pharmacology, Academy of Traditional Chinese Medicine and Materia Medica of Jilin Province, Changchun.
Chen
X G
XG
Zhang
W
W
D000818:Animals; D000999:Antlers / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003670:Deer; D008099:Liver / Q000378:metabolism*; D008419:Materia Medica; D011073:Polyamines / Q000302:isolation & purification / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D015138:RNA, Nuclear / Q000096:biosynthesis
D008419:Materia Medica; D011073:Polyamines; D015138:RNA, Nuclear; D012313:RNA; D012321:DNA-Directed RNA Polymerases