Enkephalin, dynorphin and substance P in postmortem substantia nigra from normals and schizophrenic patients.
Three peptide neuromodulators that are found in high concentration in the substantia nigra: dynorphin A 1-8, met5-enkephalin-arg6-gly7-leu8 and substance P, were measured by specific radioimmunoassays in nigral tissue from normals and schizophrenics postmortem. Substance P and dynorphin were unchanged between the two groups. However, the proenkephalin-derived peptide was significantly elevated in the schizophrenic group. The immunoreactivity was identified as authentic met5-enkephalin-arg6-gly7-leu8 by high pressure liquid chromatography. The data suggest that a different set of regulatory controls exists for nigral enkephalin peptides as compared to dynorphin and substance P, and that the former system may be disordered in schizophrenia.
Iadarola
M J
MJ
Neurobiology and Anesthesiology Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
Ofri
D
D
Kleinman
J E
JE
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000378:metabolism*; D011863:Radioimmunoassay; D012559:Schizophrenia / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism*; D013378:Substantia Nigra / Q000378:metabolism*
D010446:Peptide Fragments; D013373:Substance P; D004744:Enkephalin, Methionine; D004399:Dynorphins; C026770:dynorphin (1-8); C033453:enkephalin-Met, Arg(6)-Gly(7)-Leu(8)-