Determination of acidic metabolites of biogenic amines in human aqueous humour by gas chromatography--negative ion chemical ionisation mass spectrometry.
The concentrations of acidic metabolites derived from the biogenic amines o-, m-, and p-tyramines (o-, m-, and p-hydroxyphenylacetic acids), p-octopamine/p-synephrine (p-hydroxymandelic acid), and dopamine (homovanillic acid and 3,4-dihydroxyphenylacetic acid) were measured in human aqueous humour obtained from patients undergoing elective surgery for cataract removal or for trabeculectomy as a treatment for chronic open-angle glaucoma. There were no clear differences in the pattern of metabolism of neurotransmitters between the two groups. An unexpected finding was that the o-tyramine metabolite, o-hydroxyphenylacetic acid, was present in aqueous humour.
Watson
D G
DG
Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, Scotland, U.K.
McGhee
C N
CN
Midgley
J M
JM
Zhou
P
P
Doig
W M
WM
D000143:Acids / Q000378:metabolism*; D000328:Adult; D000368:Aged; D001082:Aqueous Humor / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008875:Middle Aged; D009655:Octopamine / Q000378:metabolism*; D009994:Osmolar Concentration; D014439:Tyramine / Q000378:metabolism*; D014822:Vitreous Body / Q000378:metabolism
D000143:Acids; D009655:Octopamine; D004298:Dopamine; D014439:Tyramine