[Methodological approaches to detecting mRNA in histological sections].
This paper reviews methodological approaches to detection of mRNA on histological sections using in situ hybridization. Alongside with the review of literature we present a detailed description of our own modification of this technique, based on the use of paraffin sections of the material fixed by replacement in the frozen state. Particular attention is given to methodological problems facing the researcher who is using in situ hybridization for the first time.
Luk'ianov
S A
SA
Zaraĭskiĭ
A G
AG
D000165:Acridine Orange; D000818:Animals; D001345:Autoradiography / Q000379:methods; D015342:DNA Probes / Q000302:isolation & purification; D006651:Histocytochemistry / Q000295:instrumentation / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods; D009693:Nucleic Acid Hybridization; D015347:RNA Probes / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods
D015342:DNA Probes; D015347:RNA Probes; D012333:RNA, Messenger; D000165:Acridine Orange
Metodicheskie podkhody k vyiavleniiu mRNK na gistologicheskikh srezakh.