Fast atom bombardment mass spectrometric analysis of arginine-containing neuropeptides.
A new procedure using fast atom bombardment mass spectrometry for the determination of arginine residues in neuropeptides is described. The technique is based on the modification of the arginine side-group with 1,2-cyclohexanedione. This novel procedure may have a potential use in the study of neuropeptides and their processing and degrading enzymes.
Silberring
J
J
Department of Pharmacology, University of Uppsala, Sweden.
Nyberg
F
F
D000595:Amino Acid Sequence; D004399:Dynorphins / Q000737:chemistry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013373:Substance P / Q000737:chemistry
D009479:Neuropeptides; D009842:Oligopeptides; D010446:Peptide Fragments; C069748:dynorphin B (5-9); D013373:Substance P; C036877:substance P (1-7); D004399:Dynorphins; C042623:tyrosyl-glycyl-glycyl-phenylalanyl-leucyl-arginine