IEF analysis of HLA molecules immunoprecipitated by putative anti-class I-like alloantisera.
Two human alloantisera, previously described as possibly detecting new beta 2-microglobulin associated proteins, selectively expressed on HLA-A2 and HLA-A1 phytohaemagglutinin (PHA)-activated lymphocytes, immunoprecipitated only molecules with the same isoelectric point as HLA-A1 and A2 products. This result suggests that the selected alloantisera do not react with the products of an HLA class I locus different from ABC but probably recognize a new epitope arising on HLA-A molecules upon conformational changes consequent to cell activation.
Crepaldi
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T
Dipartimento di Genetica, Università di Torino, Italy.
D'Alfonso
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S
Richiardi
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P
D000961:Antilymphocyte Serum; D000939:Epitopes; D006680:HLA Antigens / Q000302:isolation & purification*; D015788:HLA-A1 Antigen / Q000302:isolation & purification; D015789:HLA-A2 Antigen / Q000302:isolation & purification; D006801:Humans; D007518:Isoantibodies; D007525:Isoelectric Focusing; D008213:Lymphocyte Activation; D011233:Precipitin Tests
D000961:Antilymphocyte Serum; D000939:Epitopes; D006680:HLA Antigens; D015788:HLA-A1 Antigen; D015789:HLA-A2 Antigen; D007518:Isoantibodies