Presence of an SH2 domain in the actin-binding protein tensin.
The molecular cloning of the complementary DNA coding for a 90-kilodalton fragment of tensin, an actin-binding component of focal contacts and other submembraneous cytoskeletal structures, is reported. The derived amino acid sequence revealed the presence of a Src homology 2 (SH2) domain. This domain is shared by a number of signal transduction proteins including nonreceptor tyrosine kinases such as Abl, Fps, Src, and Src family members, the transforming protein Crk, phospholipase C-gamma 1, PI-3 (phosphatidylinositol) kinase, and guanosine triphosphatase-activating protein (GAP). Like the SH2 domain found in Src, Crk, and Abl, the SH2 domain of tensin bound specifically to a number of phosphotyrosine-containing proteins from v-src-transformed cells. Tensin was also found to be phosphorylated on tyrosine residues. These findings suggest that by possessing both actin-binding and phosphotyrosine-binding activities and being itself a target for tyrosine kinases, tensin may link signal transduction pathways with the cytoskeleton.
Davis
S
S
Division of Cellular and Molecular Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
Lu
M L
ML
Lo
S H
SH
Lin
S
S
Butler
J A
JA
Druker
B J
BJ
Roberts
T M
TM
An
Q
Q
Chen
L B
LB
D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002642:Chick Embryo; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction; D000070876:Tensins; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D000199:Actins; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D010446:Peptide Fragments; D000070876:Tensins; D019000:Phosphotyrosine; D014443:Tyrosine; D004247:DNA; D011505:Protein-Tyrosine Kinases