A microtiter plate-based assay for phosphoenolpyruvate carboxylase.
A sensitive, quantitative assay for phosphenolpyruvate carboxylase which utilizes microtiter plates is described. The assay depends upon the production of a colored compound in the reaction between oxaloacetate, the product of the phosphoenolpyruvate reaction, and the dye Fast Violet B. The method is particularly appropriate for monitoring chromatographic eluates and its utility for this purpose is demonstrated by the detection of phosphoenolpyruvate carboxylase in fractions of crude maize extract separated by size-exclusion chromatography.
Cockburn
W
W
Botany Department, School of Biological Sciences, University of Leicester, United Kingdom.
Whitelam
G C
GC
Slocombe
S P
SP
McKee
R A
RA
D003979:Diazonium Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008832:Microchemistry / Q000379:methods; D010730:Phosphoenolpyruvate Carboxylase / Q000032:analysis*; D012044:Regression Analysis; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods; D013194:Staining and Labeling
D003979:Diazonium Compounds; C067037:Fast Violet B; D010730:Phosphoenolpyruvate Carboxylase