[Exposure of the major immunodominant epitope of the gp51 envelope protein of bovine leukosis virus on the surface of the hepatitis B core antigen capsid].
Insertion of 48 amino acid long sequence of envelope protein gp51 of bovine leukemia virus (BLV), located from position 56 till 103 of mature protein, into Pro144 position of hepatitis B core antigen (HBcAg) leads to the formation of chimeric capsids. These capsids preserve morphology of intact HBcAg but expose on their outer surface BLV epitopes which are localised in the inserted gp51 fragment and responsible for the recognition of chimeras by monoclonal anti-gp51 antibodies MAK14. The anti-genicity of gp51 epitopes within chimeric capsids is not disturbed after shortening of C terminal part of inserted gp51 fragment by deletion of amino acids 73-103. The resulting chimeras show the same capsid-forming ability as well as HBcAg and gp51 antigenic properties.
Ul'rikh
R
R
Borisova
G P
GP
Siakkou
Kh
Kh
Plattser
K
K
Ose
V P
VP
Berzin'
I G
IG
Dreĭlinia
D E
DE
Pushko
P M
PM
Tsibinogin
V V
VV
Pumpen
P P
PP
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002213:Capsid / Q000276:immunology* / Q000648:ultrastructure; D002678:Chimera; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D005779:Immunodiffusion; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008854:Microscopy, Electron; D013601:T-Lymphocytes / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006512:Hepatitis B Core Antigens; D014759:Viral Envelope Proteins
Eksponirovanie mazhornogo immunodominantnogo épitopa obolochechnogo belka gp51 virusa leĭkoza krupnogo rogatogo skota na poverkhnosti kapsid kor-antigena virusa gepatita B.