Augmentation of haptoglobin production in Hep3B cell line by a nuclear factor NF-IL6.
The nuclear factor NF-IL6 had been suggested to be responsible for the IL-6-mediated induction of several acute-phase proteins. To obtain evidence for the involvement of NF-IL6 in the induction of acute-phase proteins, we introduced the NF-IL6 gene and its truncated mutant (delNFIL6) gene into a hepatoma cell line Hep3B. Then, we examined the effect of the overproduced NF-IL6 and delNFIL6 on the expression of haptoglobin, fibrinogen and albumin. As a result, basal production as well as induction of haptoglobin by IL-6 were augmented by the expression of NF-IL6, whereas delNFIL6 blocked the production of haptoglobin, fibrinogen and albumin.
Natsuka
S
S
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Isshiki
H
H
Akira
S
S
Kishimoto
T
T
D000209:Acute-Phase Proteins / Q000096:biosynthesis; D000595:Amino Acid Sequence; D015153:Blotting, Western; D022762:CCAAT-Enhancer-Binding Proteins; D002460:Cell Line; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D006242:Haptoglobins / Q000235:genetics* / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014162:Transfection
D000209:Acute-Phase Proteins; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D006242:Haptoglobins; D015850:Interleukin-6; D009687:Nuclear Proteins; D014157:Transcription Factors