Identification and initial characterization of glucose-repressible promoters of Streptococcus mutans.
Three catabolite-repressible promoters from Streptococcus mutans have been isolated. These promoters were identified by utilizing the vector pRQ200 which contains a promoterless amylase-encoding gene, a Gram- origin of replication, and an erythromycin-resistance determinant. A library of S. mutans DNA was constructed in pRQ200, amplified in Escherichia coli and integrated by Campbell-type insertion into the S. mutans chromosome following transformation. Colonies exhibiting amylase production on media lacking an extraneous carbohydrate source were screened for diminished amylase production on media containing glucose. The effect of glucose on these promoters has been characterized using a quantitative spectrophotometric assay of amylase activity.
Lane
M A
MA
Department of Biological Sciences, University of Maryland Baltimore County 21228.
Bayles
K W
KW
Yasbin
R E
RE
D000681:Amylases / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D005822:Genetic Vectors; D005947:Glucose / Q000494:pharmacology*; D007700:Kinetics; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D013295:Streptococcus mutans / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
D000681:Amylases; D005947:Glucose