Anatomical relationship between neuropeptide-containing fibers and efferent vagal neurons projecting to the rat corpus.
Injections of the retrograde tracers into the posterior surface of the stomach at the greater curvature resulted in labelling of the right half of the dorsal motor nucleus of the vagus. Whereas injections into the anterior and posterior surfaces of the corpus resulted in bilateral labelling in the medulla. Immunocytochemical staining of the labelled sections using antisera to substance P was confined to a dense network of fibers within the dorsal motor nucleus of the vagus and the nucleus tractus solitarius with no cell bodies being detected. Calcitonin gene-related peptide-immunoreactivity was detected in nerve fibers in the nucleus tractus solitarius and cell bodies of the hypoglossal nucleus. Finally, neuropeptide Y-immunoreactivity was confined to nerve fibers within the vagal complex. Of the neurons labelled by the retrograde tracers injected into the corpus all were in close spatial contact with fibers containing substance P-immunoreactivity. A smaller number were associated with neuropeptide Y-containing fibers with a few adjacent to calcitonin gene-related peptide-immunoreactive fibers. These results indicate that substance P and neuropeptide Y may directly regulate efferent neurons controlling gastric motility and acid secretion. Calcitonin gene-related peptide, however, is unlikely to directly modulate the cell bodies of the neurons in the dorsal motor nucleus but may modulate the dendrites from these neurons in the nucleus tractus solitarius.
Buchan
A M
AM
Department of Physiology, University of British Columbia, Vancouver, Canada.
Kwok
Y N
YN
Pederson
R A
RA
D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D004525:Efferent Pathways / Q000166:cytology; D007150:Immunohistochemistry; D009046:Motor Neurons / Q000166:cytology*; D009476:Neurons, Efferent / Q000166:cytology*; D009479:Neuropeptides; D011849:Radioactive Tracers; D051381:Rats; D013270:Stomach / Q000033:anatomy & histology / Q000294:innervation*; D013373:Substance P; D014630:Vagus Nerve / Q000166:cytology*
D009479:Neuropeptides; D011849:Radioactive Tracers; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide