title: Low-potassium UW solution for lung preservation. Comparison with regular UW, LPD, and Euro-Collins solutions.
abstract: University of Wisconsin solution has been used successfully in clinical kidney and liver preservation. The object of this study was to determine if low-potassium UW (LPUW) solution could be applied to pulmonary preservation. Rabbit lungs were stored after hypothermic pulmonary artery (PA) flush with four different solutions (group 1: low-potassium dextran (LPD) solution, group 2: high-potassium UW (HPUW) solution, group 3: LPUW solution, group 4: modified Euro-Collins (E-C) solution). The lungs were preserved at 10 degrees C for 30 hr and evaluated in an ex vivo ventilation/perfusion apparatus using fresh pooled venous rabbit blood. Mean PA flush pressures (MFP) during harvesting were significantly lower in groups 1 and 3 (8.1 +/- 1.0 mmHg and 7.3 +/- 0.6 mmHg, respectively; mean +/- SEM) than in groups 2 and 4 (15.5 +/- 1.7 mmHg and 12.3 +/- 0.9 mmHg, respectively). Lungs in groups 1 and 3 showed significantly higher PaO2 (103.5 +/- 8.0 mmHg and 89.3 +/- 7.2 mmHg) than groups 2 and 4 (48.3 +/- 7.7 mmHg, 66.7 +/- 4.7 mmHg). Groups 1 and 3 showed significantly lower wet/dry weight (W/D) ratios after reperfusion (6.21 +/- 0.15 and 6.39 +/- 0.23) than groups 2 and 4 (7.70 +/- 0.57 and 7.13 +/- 0.21, respectively). There were no significant differences in MFP, PaO2, PaCO2, mean pulmonary artery pressure, or W/D ratio between groups 1 and 3. These results suggest that LPUW solution may be as beneficial as LPD solution for pulmonary arterial flush and lung preservation.
authors.affiliation: Department of Surgery, University of Toronto, Ontario, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: J D
authors.initials: JD
authors.lastname: Puskas
authors.forename: E
authors.initials: E
authors.lastname: Mayer
authors.forename: P F
authors.initials: PF
authors.lastname: Cardoso
authors.forename: S Q
authors.initials: SQ
authors.lastname: Shi
authors.forename: W
authors.initials: W
authors.lastname: Wisser
authors.forename: A S
authors.initials: AS
authors.lastname: Slutsky
authors.forename: G A
authors.initials: GA
authors.lastname: Patterson
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001784:Blood Gas Analysis; D003911:Dextrans; D005978:Glutathione; D006982:Hypertonic Solutions; D007328:Insulin; D008168:Lung; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011188:Potassium / Q000032:analysis*; D011817:Rabbits; D011887:Raffinose; D012996:Solutions / Q000737:chemistry*
chemical_list: D003911:Dextrans; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D011188:Potassium