Carbohydrates associated with the cell coat surrounding cells of the rainbow trout saccular macula as revealed by lectin probes.
The luminal surface of the saccular macula in the rainbow trout is covered with a glycoconjugate-rich cell coat. The aim of this study was to identify specific carbohydrate moieties present in this coat, using biotinylated lectins as probes. Saccular tissues were fixed in Karnovsky's fixative for 2 h at 1-2 degrees C, followed by incubation with biotinylated lectins for 12-16 h at 25 degrees C. Lectin binding was visualized by performing avidin-biotin-peroxidase reactions. As controls, specimens were reacted with solutions of lectins preincubated with their specific inhibitory sugars. Staining was observed that was consistent with the presence of glucose, galactose, fucose, mannose, N-acetylglucosamine, N-acetylneuraminic acid, and N-acetylgalactosamine in the cell coat. The variability in the intensity of staining associated with the lectin-carbohydrate complexes suggests quantitative differences among the various carbohydrate moieties detected. The presence of these carbohydrates in the cell coat of the trout saccular macula also suggests biochemical similarities between cell coats in teleost and mammalian inner ear structures.
Khan
K M
KM
Department of Otolaryngology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Hatfield
J S
JS
Drescher
D G
DG
D000818:Animals; D001710:Biotin; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000378:metabolism; D037102:Lectins; D008854:Microscopy, Electron; D012444:Saccule and Utricle / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D013194:Staining and Labeling; D014337:Trout / Q000378:metabolism*
D037102:Lectins; D001710:Biotin