Regulation of acute phase reaction by transforming growth factor beta in cultured murine hepatocytes.
Transforming growth factor-beta (TGF beta 1), a multipotent immunoregulatory peptide produced by human platelets, has been shown to stimulate the synthesis of fibrinogen, contrapsin, complement component C3, and alpha-1-proteinase inhibitor by murine hepatocytes cultured for 2 days in DMEM containing 1 microM insulin and dexamethasone and 0.2% BSA. In the range of 10 pg to 10 ng/ml TGF-beta 1 did not elicit any change in albumin secretion. Two main inflammatory cytokines: interleukin-6 (IL-6) and interleukin-1 (IL-1), known to stimulate two different subsets of murine acute phase plasma proteins, failed to increase contrapsin and alpha-1-proteinase inhibitor production. Epidermal growth factor (EGF) in the concentration 1 ng to 10 ng/ml effectively counteracted the stimulatory effect of TGF-beta 1 on acute phase protein production. TGF-beta 1-induced fibrinogen protein levels were associated with increased beta-fibrinogen mRNA content. TGF-beta 1 appears to be an additional physiological factor responsible for the direct stimulation of normal mouse hepatocytes to acute phase response.
Rokita
H
H
Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Szuba
K
K
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000418:Albumins / Q000096:biosynthesis; D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003176:Complement C3 / Q000096:biosynthesis; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005340:Fibrinogen / Q000096:biosynthesis; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015843:Serpins; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014361:Trypsin Inhibitors / Q000096:biosynthesis; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis
D000209:Acute-Phase Proteins; D000418:Albumins; D003176:Complement C3; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D015843:Serpins; D016212:Transforming Growth Factor beta; D014361:Trypsin Inhibitors; D000515:alpha 1-Antitrypsin; D003907:Dexamethasone; C033691:contrapsin; D005340:Fibrinogen