The effects of nanoliter ejections of lidocaine into the pontomedullary reticular formation on cortically induced rhythmical jaw movements in the guinea pig.
In the ketamine/urethane anesthetized guinea pig, electromyographic (EMG) responses of the anterior digastric muscle were studied when loci within the lower brainstem were microejected with lidocaine (2%) during rhythmical jaw movements (RJMs) evoked by repetitive electrical stimulation of the masticatory area of the cortex. The area investigated was between the trigeminal motor nucleus (Mot V) and the rostral pole of the inferior olive. Microejections of lidocaine, contralateral to the cortical stimulus site, into the ventral-medial portion of Mot V where digastric motoneurons are known to be located, resulted in reduction or complete abolishment of the digastric EMG activity ipsilateral to the ejection with no effective change in mean cycle duration (CD) or mean percent normalized integrated amplitude of the contralateral digastric EMG. Microejections of lidocaine, contralateral to the cortical stimulus site, into the ponto-medullary reticular formation in areas that included portions of the caudal nucleus pontis caudalis (PnC), nucleus gigantocellularis (GC), medial nucleus parvocellularis (PCRt), and dorsal paragigantocellularis (dPGC), in most cases produced a bilateral reduction in the mean normalized integrated amplitude and a bilateral increase in the mean cycle duration. In these sites, the bilateral increase in mean cycle duration of digastric EMG bursts was also associated with a significant increase of coefficient of variation in CD. In many cases, microejection of lidocaine completely abolished rhythmical digastric activity, bilaterally. HRP injections into Mot V were performed to determine the locations of trigeminal premotoneurons and their relationship to effective lidocaine sites for rhythmical jaw movement suppression. Retrogradely labeled cells were found mainly in the mesencephalic nucleus of V; trigeminal principal and spinal V sensory nuclei, bilaterally; and within the intermediate and lateral regions of reticular formation, bilaterally. No labeling was found in the medial reticular formation, including the nucleus gigantocellularis and dorsal paragigantocellularis.(ABSTRACT TRUNCATED AT 400 WORDS)
Chandler
S H
SH
Department of Kinesiology, School of Dentistry, University of California, Los Angeles 90024.
Turman
J
J
Salem
L
L
Goldberg
L J
LJ
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000502:physiology; D004576:Electromyography; D006168:Guinea Pigs; D006735:Horseradish Peroxidase; D007267:Injections; D008012:Lidocaine / Q000008:administration & dosage*; D008297:Male; D008409:Mastication / Q000187:drug effects* / Q000502:physiology; D009068:Movement / Q000187:drug effects; D009334:Neck Muscles / Q000187:drug effects / Q000502:physiology; D009434:Neural Pathways / Q000187:drug effects; D012154:Reticular Formation / Q000187:drug effects* / Q000502:physiology; D013238:Stereotaxic Techniques; D014278:Trigeminal Nuclei / Q000187:drug effects; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins
D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D008012:Lidocaine; D006735:Horseradish Peroxidase