title: Interleukin-1 beta decreases HLA class II expression on a glioblastoma multiforme cell line.
abstract: Antigens encoded within the major histocompatibility complex (MHC) are not normally expressed in the central nervous system (CNS), but can be induced by treatment with interferon-gamma (IFN-gamma). Other cytokines released during an inflammatory process can potentially influence MHC expression as well. One cytokine of interest is interleukin-1 (IL-1), an immunoregulatory polypeptide that is produced by macrophages and also by cells in the CNS. In this study, the effect of IL-1 beta on MHC expression in a human glioblastoma multiforme cell line, U-105 MG, has been examined. Treatment of U-105 MG with 10 U IL-1 beta/ml for a period of 5 days resulted in a decrease in constitutive cell surface HLA class II expression and limited the induction of class II by IFN-gamma. This effect was also observed on steady-state levels of class II RNA and could be neutralized with antibodies to IL-1 beta. All class II transcripts examined (HLA-DR, -DQ, and -DP alpha and beta) were affected. Class I expression was only marginally changed by IL-1 beta treatment. A minimal concentration of 1 U IL-1 beta/ml was required to reduce class II expression and a kinetics experiment indicated that U-105 MG must be treated for at least 4 days with IL-1 beta for a decrease in class II expression to be observed. This study suggests that IL-1 may play a role in limiting immunoreactivity in the CNS by limiting class II induction.
authors.affiliation: Neuroimmunology Branch, NINDS, NIH, Bethesda, MD 20892.
authors.forename: E P
authors.initials: EP
authors.lastname: Cowan
authors.forename: M L
authors.initials: ML
authors.lastname: Pierce
authors.forename: S
authors.initials: S
authors.lastname: Dhib-Jalbut
mesh_terms: D004305:Dose-Response Relationship, Drug; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D005909:Glioblastoma / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D012313:RNA / Q000032:analysis; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D012313:RNA; D007371:Interferon-gamma