title: High affinity human IgE receptor (Fc epsilon RI). Analysis of functional domains of the alpha-subunit with monoclonal antibodies.
abstract: The binding of IgE to the high affinity Fc epsilon receptor (Fc epsilon RI) on mast cells and basophils is mediated by the alpha-subunit of the tetrameric receptor complex. Based on sequence homologies, the 50-kDa alpha-subunit is a member of the immunoglobulin superfamily of proteins and has two predicted disulfide-bonded loops. Monoclonal antibodies specific for the human alpha-subunit have been identified and separated into two major classes: inhibitory and noninhibitory antibodies. Inhibitory antibodies (i.e. 15A5) block 125I-IgE binding to a recombinant chimeric alpha-subunit (ch-alpha-protein) expressed on Chinese hamster ovary cells and immunoprecipitate 125I-labeled purified ch-alpha-protein. Noninhibitory antibodies (i.e. 22E7) immunoprecipitate both 125I-labeled ch-alpha-protein and the soluble complex of 125I-IgE cross-linked to ch-alpha-protein but do not block 125I-IgE binding to the ch-alpha-protein expressed on Chinese hamster ovary cells. Both classes of antibodies bind to natural Fc epsilon RI present on human basophils and induce histamine release from these cells. Inhibitory antibody 15A5 specifically binds to a peptide corresponding to amino acids 125-140 of the putative second domain of the alpha-subunit sequence. All the inhibitory antibodies compete with 125I-15A5 for binding to the ch-alpha-protein, indicating that these antibodies recognize inhibitory epitopes that are either identical or sterically overlapping. Noninhibitory antibodies (i.e. 22E7) do not block 125I-15A5 binding to the ch-alpha-protein. These data suggest that antibodies binding to the predicted second domain of the alpha-subunit can inhibit IgE binding to the alpha-subunit, while antibodies binding at a distance from this site do not inhibit IgE binding. These inhibitory antibodies may block IgE binding to the ch-alpha-protein by direct overlap, steric inhibition, or induced conformational changes of the receptor contact points for IgE.
authors.affiliation: Department of Molecular Genetics, Roche Research Center, Hoffman-LaRoche, Inc., Nutley, New Jersey 07110.
authors.forename: F
authors.initials: F
authors.lastname: Riske
authors.forename: J
authors.initials: J
authors.lastname: Hakimi
authors.forename: M
authors.initials: M
authors.lastname: Mallamaci
authors.forename: M
authors.initials: M
authors.lastname: Griffin
authors.forename: B
authors.initials: B
authors.lastname: Pilson
authors.forename: N
authors.initials: N
authors.lastname: Tobkes
authors.forename: P
authors.initials: P
authors.lastname: Lin
authors.forename: W
authors.initials: W
authors.lastname: Danho
authors.forename: J
authors.initials: J
authors.lastname: Kochan
authors.forename: R
authors.initials: R
authors.lastname: Chizzonite
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000378:metabolism*; D001491:Basophils / Q000276:immunology; D002678:Chimera; D005455:Fluorescent Antibody Technique; D006636:Histamine Release; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007073:Immunoglobulin E / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D046911:Macromolecular Substances; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008970:Molecular Weight; D051381:Rats; D011961:Receptors, Fc / Q000235:genetics / Q000378:metabolism*; D017455:Receptors, IgE
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000944:Antigens, Differentiation, B-Lymphocyte; D046911:Macromolecular Substances; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E