A general procedure for evaluation of immunological relevance of synthetic peptides: peptides synthesized on paper in enzyme-linked immunosorbent assay.
Multiple continuous-flow solid-phase peptide synthesis has been adapted for synthesis of peptides on a cellulose carrier (Whatman 3MM paper). Paper-bound synthetic peptides that represent antigenic determinants of particular proteins detected antibodies against the respective proteins in an enzyme-linked immunosorbent assay. The method is applied to the synthesis, and use in site-directed serology, of four peptides derived from the gp41 glycoprotein of HIV, the Epstein-Barr virus-determined nuclear antigen-1 and VCA proteins of the Epstein-Barr virus, and the early region of human papillomavirus type 11.
Krchnák
V
V
Research Institute for Feed Supplements and Veterinary Drugs, Jílové u Prahy, Czechoslovakia.
Vágner
J
J
Novák
J
J
Suchánková
A
A
Roubal
J
J
D000595:Amino Acid Sequence; D000906:Antibodies / Q000032:analysis; D002482:Cellulose; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000276:immunology; D012699:Serology / Q000379:methods
D000906:Antibodies; D000939:Epitopes; D010455:Peptides; D002482:Cellulose