title: Cell kinetic analysis of human brain tumors by in situ double labelling with bromodeoxyuridine and iododeoxyuridine.
abstract: Fifty-seven patients with brain tumors (29 gliomas, 23 meningiomas, 5 miscellaneous) received infusions of intravenous iododeoxyuridine (IUdR) and bromodeoxyuridine (BUdR) 1-5 hr apart shortly before tumor removal. Excised tumor specimens were stained sequentially for BUdR and IUdR. The percentage of BUdR-labelled cells was determined to establish the labelling index (LI), or S-phase fraction, and the ratio of cells labelled only with IUdR to cells labelled with BUdR or with BUdR and IUdR was determined to calculate the duration of S-phase (Ts) and the potential doubling time (Tp) of each tumor. The BUdR LIs varied from less than 1% to 20%, reflecting the malignancy of each tumor. Despite the difference in LIs, however, Ts was fairly uniform (mean +/- SD, 8.7 +/- 2.0 hr). Tp varied from 2 days to more than 1 month and correlated closely with the BUdR LIs (Tp = 23/LI0.93; r2 = 0.91). Double-labelling studies with IUdR and BUdR allow the S-phase fraction, Ts and Tp to be determined from a single biopsy specimen and thus provide more useful information on the growth characteristics of individual tumors than can be obtained by single-labelling studies with BUdR.
authors.affiliation: Department of Neurological Surgery, School of Medicine, University of California, San Francisco 94143.
authors.forename: T
authors.initials: T
authors.lastname: Hoshino
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: A
authors.initials: A
authors.lastname: Asai
authors.forename: M
authors.initials: M
authors.lastname: Shibuya
authors.forename: M D
authors.initials: MD
authors.lastname: Prados
authors.forename: B A
authors.initials: BA
authors.lastname: Dodson
authors.forename: R L
authors.initials: RL
authors.lastname: Davis
authors.forename: C B
authors.initials: CB
authors.lastname: Wilson
mesh_terms: D001932:Brain Neoplasms / Q000473:pathology*; D001973:Bromodeoxyuridine; D002453:Cell Cycle; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007065:Idoxuridine; D008579:Meningioma / Q000473:pathology*
chemical_list: D001973:Bromodeoxyuridine; D007065:Idoxuridine