[Hybridase cleavage of RNA. V. Complementary precision of cleavage].
The efficiency of the cleavage of RNA involved in perfect as well as imperfect hybrid duplexes composed of three components: (1) homogeneous RNA's or polyribonucleotides; (2) corresponding complementary synthetic oligodeoxyribonucleotides; (3) E. coli RNase H was investigated. The predominant RNA hydrolysis was shown to take place within the perfect hybrid duplexes formed by the target RNA and the complementary oligodeoxyribonucleotide probes. RNase H was found to cleave effectively a number of imperfect hybrid duplexes containing a central base pair mismatch.
Metelev
V G
VG
Krynetskaia
N F
NF
Volkov
E M
EM
Oretskaia
T S
TS
Shabarova
Z A
ZA
D001345:Autoradiography; D001483:Base Sequence; D002384:Catalysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes; D012313:RNA / Q000378:metabolism*; D016914:Ribonuclease H / Q000378:metabolism*
D015345:Oligonucleotide Probes; D012313:RNA; D016914:Ribonuclease H
Gibridaznoe rasshcheplenie RNK. V. Komplementatsionnaia tochnost' rasshchepleniia.