title: Fumarate or a fumarate metabolite restores switching ability to rotating flagella of bacterial envelopes.
abstract: Flagella of cytoplasm-free envelopes of Escherichia coli or Salmonella typhimurium can rotate in either the counterclockwise or clockwise direction, but they never switch from one direction of rotation to another. Exogenous fumarate, in the intracellular presence of the chemotaxis protein CheY, restored switching ability to envelopes, with a concomitant increase in clockwise rotation. An increase in clockwise rotation was also observed after fumarate was added to partially lysed cells of E. coli, but the proportion of switching cells remained unchanged.
authors.affiliation: Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Barak
authors.forename: M
authors.initials: M
authors.lastname: Eisenbach
mesh_terms: D002462:Cell Membrane / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects*; D002633:Chemotaxis / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects*; D005407:Flagella / Q000187:drug effects*; D005650:Fumarates / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D005650:Fumarates; C032005:fumaric acid