Structural organization of pBC1, a cryptic plasmid from Bacillus coagulans.
The complete nucleotide sequence of the Bacillus coagulans plasmid pBC1 was determined. The sequence revealed an open reading frame encoding a polypeptide of 259 amino acids. This open reading frame shows sequence similarity to genes coding for replication-associated proteins in a group of gram-positive bacterial plasmids known to replicate via single-stranded intermediates. A region required for replication in cis, when the intact replicon is supplied in trans, was identified as well.
De Rossi
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E
Department of Genetics and Microbiology, A. Buzzati Traverso, University of Pavia, Italy.
Milano
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A
Brigidi
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P
Bini
F
F
Riccardi
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G
D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics*; D001483:Base Sequence; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012093:Replicon