[The isolation and characteristics of monoclonal antibodies to the HNK-1 antigen of natural killers].
Monoclonal antibodies to the HNK-1 differentiated antigen of natural killer cells have been obtained. A glycoprotein of the white human brain connected with myelin was used as an antigen for immunization of mice. The monoclonal antibodies obtained are shown to reduce the cytotoxic activity of a human blood mononuclear fraction as much as by 65% in relation to the human lymphoblastoma cell culture K-562. Their interaction with the surface antigen of mononuclears was shown by immunofluorescent method. Monoclonal antibodies belong to the class of immunoglobulins M.
Aksenova
N N
NN
Ivanov
V A
VA
Kovaleva
Z V
ZV
Pleskach
V A
VA
Firulina
I I
II
Fel'
V Ia
VIa
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D000943:Antigens, Differentiation / Q000276:immunology*; D001921:Brain / Q000276:immunology; D018959:CD57 Antigens; D003601:Cytotoxicity Tests, Immunologic; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007124:Immunoenzyme Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018959:CD57 Antigens; D006023:Glycoproteins; D009419:Nerve Tissue Proteins
Poluchenie i kharakteristika monoklonal'nykh antitel k HNK-1-antigenu estestvennykh killerov.