title: Partial purification of human prostatic 5 alpha-reductase (3-oxo-5 alpha-steroid:NADP+ 4-ene-oxido-reductase; EC 1.3.1.22) in a stable and active form.
abstract: Human hyperplastic prostate tissue was homogenised in high ionic strength buffer and the post nuclear homogenate was incubated with 0.8% octyl glucoside and bovine brain lipids. Dialysis of the resulting liposome suspension yielded a preparation in which 5 alpha-reductase was active and stable for at least three weeks and showed an increase in specific activity (Vmax +/- SD = 48.9 +/- 7.4 pmol DHT/mg protein/ml) over that of the starting homogenate (Vmax +/- SD = 5.6 +/- 1.5 pmol DHT/mg protein/min) of 8.7 times.
authors.affiliation: University Department of Surgery (WGH), Western General Hospital, Edinburgh, Scotland.
authors.forename: N S
authors.initials: NS
authors.lastname: Sargent
authors.forename: F K
authors.initials: FK
authors.lastname: Habib
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D003902:Detergents / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008055:Lipids; D008297:Male; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000201:enzymology; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000201:enzymology; D011480:Protease Inhibitors / Q000494:pharmacology
chemical_list: D003902:Detergents; D008055:Lipids; D011480:Protease Inhibitors; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase