cDNA cloning of a novel testis-specific calcineurin B-like protein.
A cDNA clone encoding a novel calcineurin B-like protein (CBLP) was isolated from a rat testis cDNA library by cross-hybridization with a calcineurin B cDNA probe. CBLP is composed of 176 amino acids and contains four putative Ca(2+)-binding domains. The overall predicted amino acid sequence homology between CBLP and calcineurin B is 82%. Northern blots showed that transcripts for CBLP are specifically and highly expressed in the testis.
Mukai
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H
Department of Pharmacology, Kobe University School of Medicine, Japan.
Chang
C D
CD
Tanaka
H
H
Ito
A
A
Kuno
T
T
Tanaka
C
C
D000595:Amino Acid Sequence; D000818:Animals; D029681:Arabidopsis Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D019703:Calcineurin; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D015723:Gene Library; D008297:Male; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000201:enzymology*; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; D019703:Calcineurin; D002135:Calcium-Binding Proteins; D002148:Calmodulin-Binding Proteins; D004247:DNA; D015342:DNA Probes; D010749:Phosphoprotein Phosphatases; D012313:RNA; C484078:CBL10 protein, Arabidopsis; C484079:CBL3 protein, Arabidopsis; C483419:calcineurin B-like protein 1, Arabidopsis; C071056:Ppp3r2 protein, rat; C480758:At5g55990 protein, Arabidopsis