A protective monoclonal antibody specifically recognizes and alters the catalytic activity of schistosome triose-phosphate isomerase.
mAb M.1 was previously shown to recognize a 28-kDa Ag in all stages of the human helminth parasite, Schistosoma mansoni, and to bind to the surface membranes of newly transformed schistosomula in a transient manner. Here we demonstrate that M.1 passively transfers partial resistance (41-49%) to cercarial challenge in naive mice. Thus, the 28-kDa Ag recognized by M.1 is a putative vaccine candidate. After immunoaffinity purification, tryptic digests of the 28-kDa Ag were prepared and individual peptides were sequenced. Amino terminus sequences of tryptic peptides of the 28-kDa Ag had high (79-87%) sequence homology with the mammalian glycolytic/gluconeogenic enzyme triosephosphate isomerase (TPI). Purified, native 28-kDa Ag from adult parasites was shown to function enzymatically in an analogous manner to yeast and mammalian TPI in the reverse reaction. Addition of M.1 antibody to the enzyme reaction altered the catalytic activity of schistosome TPI. To determine the immunologic cross-reactivity of this vaccine candidate with mammalian TPI, Western blot analysis was performed and demonstrated that M.1 was immunologically specific for the schistosome enzyme.
Harn
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DA
Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115.
Gu
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Oligino
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LD
Mitsuyama
M
M
Gebremichael
A
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Richter
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D
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D007116:Immunization, Passive; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012547:Schistosoma / Q000201:enzymology* / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology; D012552:Schistosomiasis / Q000517:prevention & control; D014305:Triose-Phosphate Isomerase / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D014305:Triose-Phosphate Isomerase