Immunohistochemical identification of tubular segments in percutaneous renal biopsies.
To identify the renal cortical tubular segments involved in tubulo-interstitial disease in formalin-fixed, paraffin-embedded percutaneous kidney biopsies, we developed multiple immunolabeling protocols using segment-specific tubular markers. The present study of biopsies from patients with minimal change or thin basement membrane nephropathy provides a baseline for interpretation of histopathology. Proximal tubules were stained either by the PAS reaction or by the biotinylated Phaseolus vulgaris erythroagglutinin (PHA-E)-streptavidin-gold-silver system (brush borders black). The anti-Tamm-Horsfall (THP) antibody-immunoperoxidase (aminoethylcarbazole, AEC-IPO), and anti-epidermal cytokeratins (ECK) antibodies-immunoalkaline-Fast Blue BB methods marked the distal straight tubules and the cortical collecting system red-brown and blue, respectively. When these immunolabelings were combined, the coapplication of AEC-PO-labeled peanut agglutinin (PNA) or anti-epithelial membrane antigen antibody-AEC-IPO technique (both are markers for distal nephron) visualized the apical membranes of distal convoluted tubules. In the protocol PHA-E + PNA + THP + ECK, the tubular basement membranes were outlined by the anti-laminin antibody-AEC-IPO staining, carried out simultaneously. The protocol PNA + THP + ECK + PAS was found to be quite appropriate multiple immunolabeling method for the tubules, and is recommended for use as a tool in the study of tubulo-interstitial diseases.
Iványi
B
B
Institute of Pathology, Albert Szent-Györgyi University of Medicine, Szeged, Hungary.
Olsen
T S
TS
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods*; D007633:Keratins; D007674:Kidney Diseases / Q000175:diagnosis; D007684:Kidney Tubules / Q000737:chemistry* / Q000166:cytology / Q000473:pathology; D037102:Lectins; D008297:Male; D008875:Middle Aged; D009088:Mucoproteins; D019887:Peanut Agglutinin; D010503:Periodic Acid-Schiff Reaction; D010835:Phytohemagglutinins; D013194:Staining and Labeling; D058949:Uromodulin
D015415:Biomarkers; D037102:Lectins; D009088:Mucoproteins; D019887:Peanut Agglutinin; D010835:Phytohemagglutinins; C548612:UMOD protein, human; D058949:Uromodulin; C067494:erythroagglutinating phytohemagglutinin; D007633:Keratins