Rat C alpha catalytic subunit of the cAMP-dependent protein kinase: cDNA sequence and evidence that it is the only isoform expressed in myoblasts.
A full length cDNA clone encoding the C alpha type catalytic subunit of cAMP-dependent protein kinase was isolated from a cDNA library of differentiated rat myoblast L6 cell line. The 2137 bp clone codes for a protein of 351 amino acid residues having more than 90% sequence identity to C alpha subunits of other mammalians. The C alpha isoform was found to be the only isoform of catalytic subunits expressed in myoblast cells as was determined in Northern blot analysis.
Wiemann
S
S
Institute of Experimental Pathology, German Cancer Research Center, Heidelberg.
Voss
H
H
Kinzel
V
V
Pyerin
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002384:Catalysis; D002460:Cell Line; D004247:DNA / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D011494:Protein Kinases / Q000235:genetics*; D012313:RNA / Q000032:analysis; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D007527:Isoenzymes; D012313:RNA; D004247:DNA; D011494:Protein Kinases