Increased hyaline droplet formation in male rats exposed to decalin is dependent on the presence of alpha 2u-globulin.
A peculiar decalin-induced male rat nephropathy associated with the altered renal handling of filtered protein appears limited to the accumulation of the protein, alpha 2u-globulin. Several strains of male rats that produce alpha 2u-globulin (Fischer-344, Sprague-Dawley, Buffalo, and Norway Brown) demonstrate spontaneous renal cortical hyaline droplets which are exacerbated after exposure to decalin. In all cases, a close correlation exists between hyaline droplet formation observed histologically and alpha 2u-globulin accumulation measured biochemically. In stark contrast, the NCI-Black-Reiter strain, which does not produce measurable quantities of alpha 2u-globulin, neither forms hyaline droplets nor accumulates any filtered protein in its kidney cortex either spontaneously or after exposure to decalin. Also, female rats injected ip with male rat alpha 2u-globulin exhibit increased hyaline droplet formation and alpha 2u-globulin accumulation when treated with decalin. These data provide evidence that the presence of alpha 2u-globulin is key in understanding why this nephropathy appears unique to the male rat.
Ridder
G M
GM
Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45239-8707.
Von Bargen
E C
EC
Alden
C L
CL
Parker
R D
RD
D000510:Alpha-Globulins / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D004586:Electrophoresis; D005260:Female; D006818:Hyalin / Q000378:metabolism*; D007672:Kidney Cortex / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009281:Naphthalenes / Q000633:toxicity*; D051381:Rats; D011915:Rats, Inbred BUF; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D013045:Species Specificity
D000510:Alpha-Globulins; D009281:Naphthalenes; C013803:alpha 2u globulin; C007229:decalin