title: A human alpha-fetoprotein enhancer-binding protein, ATBF1, contains four homeodomains and seventeen zinc fingers.
abstract: We have isolated a full-length cDNA encoding a protein (ATBF1) that binds to an AT-rich motif in the human alpha-fetoprotein gene enhancer. The amino acid sequence deduced from the cDNA revealed that this is the largest DNA-binding protein (306 kDa) known to date, containing four homeodomains, 17 zinc finger motifs, and a number of segments potentially involved in transcriptional regulation. Although the exact function of this protein has not been determined, these structural features suggest that ATBF1 plays a transcriptional regulatory role.
authors.affiliation: Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Japan.
authors.forename: T
authors.initials: T
authors.lastname: Morinaga
authors.forename: H
authors.initials: H
authors.lastname: Yasuda
authors.forename: T
authors.initials: T
authors.lastname: Hashimoto
authors.forename: K
authors.initials: K
authors.lastname: Higashio
authors.forename: T
authors.initials: T
authors.lastname: Tamaoki
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004742:Enhancer Elements, Genetic; D018398:Homeodomain Proteins; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured; D016335:Zinc Fingers; D000509:alpha-Fetoproteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D000509:alpha-Fetoproteins; C071660:ZFHX3 protein, human; D004247:DNA