Endothelin receptor: a profoundly desensitizing receptor expressed in Xenopus oocytes.
Xenopus oocyte expression studies can help to define the physiological properties of a receptor and can aid in receptor cloning. Expression of an endothelin receptor in oocytes injected with brain mRNA is inferred from depolarizations induced by several endothelin-related peptides. The response is abolished by intracellular EGTA injection but not in the absence of extracellular Ca2+. It is not present in non-injected oocytes, and reverses at a potential indicating that it is mediated by an increased Cl-1 conductance. Responses display striking, long-lasting desensitization. The expressed endothelin receptor thus displays properties characteristic of several receptors coupled to changes in phosphoinositide turnover, several of which have been successfully cloned using this response as a reporter.
Shimada
S
S
Laboratory of Molecular Neurobiology, ARC/NIDA, Baltimore, MD.
Spivak
C
C
Uhl
G
G
D000818:Animals; D002118:Calcium / Q000502:physiology; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D009496:Neurotensin / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D017466:Receptors, Endothelin; D012701:Serotonin / Q000494:pharmacology; D013373:Substance P / Q000494:pharmacology; D014757:Viper Venoms / Q000494:pharmacology; D014981:Xenopus / Q000378:metabolism*
D010455:Peptides; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D014757:Viper Venoms; C057354:sarafotoxins s6; D012701:Serotonin; D013373:Substance P; D009496:Neurotensin; D002118:Calcium