title: Fluorescent studies directed towards the location of abnormal epithelial cells in cervical smears.
abstract: Cervical screening is concerned with the search for abnormal epithelial cells in smears prepared from scrapings from the uterine cervix. It is a highly skilled labour intensive operation and automated methods of detecting dyskariotic cells in cervical smears would be helpful. We report a fluorescence method of detecting abnormal cervical cells in smears and biopsies using a probe for guanidinobenzoatase. This approach has the potential for automation.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester.
authors.forename: F S
authors.initials: FS
authors.lastname: Steven
authors.forename: J
authors.initials: J
authors.lastname: Johnson
mesh_terms: D000585:Aminacrine; D001331:Automation; D015415:Biomarkers; D001706:Biopsy; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D002278:Carcinoma in Situ / Q000175:diagnosis / Q000473:pathology; D002454:Cell Differentiation; D002584:Cervix Uteri / Q000201:enzymology / Q000473:pathology*; D010450:Endopeptidases; D004848:Epithelium / Q000201:enzymology / Q000473:pathology; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D008856:Microscopy, Fluorescence; D011419:Propidium; D013194:Staining and Labeling / Q000379:methods; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000473:pathology; D014626:Vaginal Smears / Q000379:methods*
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins; D011419:Propidium; D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases