Expression of large hepatitis B envelope protein mutants using a new expression vector.
Aminoterminal deletion mutants of the gene encoding the large hepatitis B surface protein were expressed in COS cells using a new expression vector. The truncated protein showed the same intracellular retention like the wild type protein. The findings show that the secretion block of the protein is not due to its aminoterminal myristylation.
Korec
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Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Gerlich
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D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics*
D006514:Hepatitis B Surface Antigens; D009227:Myristic Acids; D014759:Viral Envelope Proteins; D019814:Myristic Acid