RNA editing of wheat mitochondrial ATP synthase subunit 9: direct protein and cDNA sequencing.
RNA editing of subunit 9 of the wheat mitochondrial ATP synthase has been studied by cDNA and protein sequence analysis. Most of the cDNA clones sequenced (95%) showed that editing by C-to-U transitions occurred at eight positions in the coding region. Consequently, 5 amino acids were changed in the protein when compared with the sequence predicted from the gene. Two edited codons gave no changes (silent editing). One of the C-to-U transitions generated a stop codon by modifying the arginine codon CGA to UGA. Thus, the protein produced is 6 amino acids shorter than that deduced from the genomic sequence. Minor forms of cDNA with partial or overedited sequences were also found. Protein sequence and amino acid composition analyses confirmed the results obtained by cDNA sequencing and showed that the major form of edited atp9 mRNA is translated.
Bégu
D
D
Institut de Biochimie Cellulaire et Neurochimie du Centre National de la Recherche Scientifique, Bordeaux, France.
Graves
P V
PV
Domec
C
C
Arselin
G
G
Litvak
S
S
Araya
A
A
D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification; D017393:RNA Editing; D000077278:RNA, Mitochondrial; D017385:Sequence Homology; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
D000596:Amino Acids; D003062:Codon; D004277:DNA, Single-Stranded; D000077278:RNA, Mitochondrial; D012313:RNA; D000251:Adenosine Triphosphatases