Effects of G-CSF, GM-CSF, and IL-5 on nuclear segmentation of neutrophils and eosinophils in congenital or acquired Pelger-Huët anomaly.
We studied the in vitro effects of granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin 5 (IL-5) on nuclear segmentation of neutrophils and eosinophils from three patients with congenital or acquired Pelger-Huët anomaly. After a 24-h incubation with G-CSF, the majority of neutrophils showed nuclear development characterized by a bilobed appearance. In contrast to neutrophils, the nuclear segmentation of eosinophils was not induced after incubation with G-CSF, GM-CSF, or IL-5. These results suggest that G-CSF plays some role in the nuclear development of neutrophils, whereas IL-5 may not have such an effect on eosinophil maturation in the individual cases studied.
Teshima
T
T
First Department of Internal Medicine, Kyushu University, Fukuoka, Japan.
Shibuya
T
T
Harada
M
M
Taniguchi
S
S
Nozaki
M
M
Mori
T
T
Mori
Y
Y
Tamai
H
H
Niho
Y
Y
D000328:Adult; D002467:Cell Nucleus / Q000648:ultrastructure*; D002478:Cells, Cultured; D004804:Eosinophils / Q000378:metabolism / Q000648:ultrastructure*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism / Q000648:ultrastructure*; D010381:Pelger-Huet Anomaly / Q000097:blood* / Q000151:congenital / Q000473:pathology; D013481:Superoxides / Q000378:metabolism
D015848:Interleukin-5; D013481:Superoxides; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor