title: Partial characterization of human amniotic membrane interferon.
abstract: 1. The molecular weight and the electrofocusing profile of human amniotic membrane interferon (IFN-AM) were determined. 2. When submitted to gel filtration, IFN-AM showed a single 26-28 kDa component; in polyacrylamide gel electrophoresis one component of 19,500. 3. In electrofocusing, IFN-AM displayed a heterogeneous profile with 5 to 7 peaks, but different from human alpha or beta IFNs. This heterogeneity was reduced by previous treatment of IFN-AM with neuraminidase. 4. IFN-AM is a sialoglycoprotein similar to human beta IFN in terms of antigenicity but different from it in electrofocusing profile.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
authors.forename: P C
authors.initials: PC
authors.lastname: Ferreira
authors.forename: C
authors.initials: C
authors.lastname: Gonzaga
authors.forename: E G
authors.initials: EG
authors.lastname: Kroon
authors.forename: J R
authors.initials: JR
authors.lastname: Dos-Santos
authors.forename: H M
authors.initials: HM
authors.lastname: Bicalho
authors.forename: R R
authors.initials: RR
authors.lastname: Golgher
mesh_terms: D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005321:Extraembryonic Membranes / Q000378:metabolism*; D006801:Humans; D007372:Interferons / Q000737:chemistry*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010920:Placenta / Q000737:chemistry*
chemical_list: D007372:Interferons