Glial fibrillary acidic protein immunoreactivity in adrenocortical and Leydig cells of the Syrian golden hamster (Mesocricetus auratus).
In an immunocytochemical investigation of the expression of glial fibrillary acidic protein (GFAP) in non-nervous system tissues ten anti-GFAP antibodies were used on a range of normal adult organs from different species. All four polyclonal and six monoclonal antibodies revealed the expression of GFAP in cells of the zona fasciculata and reticularis of the adrenal cortex and Leydig cells of the Syrian hamster. The Chinese hamster, mole, rat, mouse, guinea pig, rabbit, pig, duck and man were negative. Co-expression of immunoreactivity for GFAP and vimentin was observed in adrenocortical and Leydig cells of the Syrian hamster but there were differences in the staining patterns of these intermediate filament proteins. Expression of GFAP in adrenal cortex of Syrian hamster is confirmed by immunoblot and limited proteolysis analysis which reveal a light form which is immunochemically indistinguishable from its counterpart in the central nervous system. The results presented here suggest a new model for the study of the possible role of GFAP expression in cells known to be sites of steroid synthesis.
Maunoury
R
R
Laboratoire d'Anatomie Pathologique, Hôpital Sainte-Anne, Paris, France.
Portier
M M
MM
Léonard
N
N
McCormick
D
D
D000302:Adrenal Cortex / Q000378:metabolism*; D000818:Animals; D006224:Cricetinae; D003412:Cricetulus / Q000378:metabolism; D004372:Ducks / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D008322:Mammals / Q000378:metabolism; D008647:Mesocricetus / Q000378:metabolism*; D009418:S100 Proteins / Q000032:analysis; D013194:Staining and Labeling
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009418:S100 Proteins