title: Molecular basis of sequestration in severe and uncomplicated Plasmodium falciparum malaria: differential adhesion of infected erythrocytes to CD36 and ICAM-1.
abstract: The CD36 and ICAM-1 glycoproteins on vascular endothelial cells have been implicated as cytoadherence receptors for Plasmodium falciparum-infected erythrocytes (IRBC). Adhesion of IRBC from Thai patients with uncomplicated and severe falciparum malaria to purified CD36 or ICAM-1 and to C32 melanoma cells was compared. All malaria isolates bound to solid phase-adsorbed CD36 and to fluid-phase 125I-labeled CD36. IRBC adhesion to purified ICAM-1 varied widely, and no correlation with clinical severity of disease was observed. The cytoadherent phenotype of IRBC was modulated by selective panning on plates coated with purified CD36 or ICAM-1. IRBC selected by panning on CD36+, ICAM-1+ melanoma cells bound to cells that express surface CD36 but not to CD36-deficient cells, indicating that CD36 exerts a strong selective pressure on the IRBC cytoadherent phenotype. IRBC adhesion to CD36 and ICAM-1 suggests that P. falciparum parasites may use these receptors in vivo to promote parasite survival and immune evasion.
authors.affiliation: Department of Medicine, Walter Reed Army Medical Center, Washington, DC.
authors.forename: C F
authors.initials: CF
authors.lastname: Ockenhouse
authors.forename: M
authors.initials: M
authors.lastname: Ho
authors.forename: N N
authors.initials: NN
authors.lastname: Tandon
authors.forename: G A
authors.initials: GA
authors.lastname: Van Seventer
authors.forename: S
authors.initials: S
authors.lastname: Shaw
authors.forename: N J
authors.initials: NJ
authors.lastname: White
authors.forename: G A
authors.initials: GA
authors.lastname: Jamieson
authors.forename: J D
authors.initials: JD
authors.lastname: Chulay
authors.forename: H K
authors.initials: HK
authors.lastname: Webster
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D018955:CD36 Antigens; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism* / Q000469:parasitology; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D008288:Malaria / Q000097:blood*; D008545:Melanoma; D010963:Plasmodium falciparum / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D018955:CD36 Antigens; D015815:Cell Adhesion Molecules; D011956:Receptors, Cell Surface; D018799:Intercellular Adhesion Molecule-1