Stimulation of biosynthesis of nerve growth factor by acidic fibroblast growth factor in cultured mouse astrocytes.
Bovine acidic and basic fibroblast growth factors (aFGF and bFGF) dose-dependently stimulated the release of nerve growth factor (NGF) in a culture medium of mouse astrocytes. The NGF concentration in the medium started to increase compared to that of the control cultures 4 h later and was further sustained for 24 h when aFGF was contained. The content of NGF mRNA in the astrocytes treated with aFGF peaked at eightfold over the control level after 4 h. The astrocytes did not proliferate until after 72 h when treated with FGFs under the conditions employed. These results indicate that aFGF stimulates the biosynthesis of NGF in cultured astrocytes without promoting cell proliferation.
Ono
T
T
Research Laboratories, Yoshitomi Pharmaceutical Industries, Ltd., Saitana, Japan.
Saito
H
H
Kishimoto
T
T
Okumoto
T
T
Miyamoto
K
K
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D001921:Brain / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005333:Fetus; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008813:Mice, Inbred ICR; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D009414:Nerve Growth Factors; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1