Induction of morphological change by tyrosine kinase inhibitors in Rous sarcoma virus-transformed rat kidney cells.
Erbstatin and methyl 2,5-dihydroxycinnamate, related tyrosine kinase inhibitors, induced a morphological change in temperature-sensitive Rous sarcoma virus-transformed rat kidney (RSVts-NRK) that brought the cells close to the morphology of their normal counterpart. Erbstatin did not change the morphology of normal or Kirsten sarcoma virus-transformed rat kidney cells. Erbstatin also inhibited morphological transformation of RSVts-NRK cells induced by a shifting in temperature. Actin stress fibres were observed only in normal cells and not in transformed cells. Erbstatin induced stress fibre organization in transformed cells. Erbstatin and methyl 2,5-dihydroxycinnamate increased fibronectin gene expression in RSV-transformed cells. Thus, tyrosine kinase inhibitors induced normal phenotypes specifically in v-src-expressing cells.
Umezawa
K
K
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Tanaka
K
K
Hori
T
T
Abe
S
S
Sekizawa
R
R
Imoto
M
M
D000199:Actins / Q000378:metabolism; D001358:Avian Sarcoma Viruses; D015152:Blotting, Northern; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002934:Cinnamates / Q000494:pharmacology*; D005353:Fibronectins / Q000235:genetics; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation; D006873:Hydroquinones / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D012313:RNA / Q000032:analysis
D000199:Actins; D002934:Cinnamates; D005353:Fibronectins; D005456:Fluorescent Dyes; D006873:Hydroquinones; C067472:methyl 2,5-dihydroxycinnamate; D012313:RNA; D011505:Protein-Tyrosine Kinases; C048212:erbstatin