Inhibition of experimental autoimmune uveitis by retinal photoreceptor antigens coupled to spleen cells.
Experimental autoimmune uveitis (EAU) and experimental autoimmune pinealitis (EAP) are CD4+ T cell-mediated inflammatory diseases of the uveal tract and retina of the eye and of the pineal gland. EAU and EAP can be induced by several retinal autoantigens including S-antigen (S-Ag) and interphotoreceptor retinoid binding protein (IRBP). In this study we investigated the effect of intravenous administration of S-Ag and IRBP coupled to syngeneic spleen cells on the development of EAU and EAP. Injection of S-Ag or IRBP coupled to spleen cells 5 days prior to immunization with native S-Ag or IRBP, respectively, was effective in preventing the induction of EAU and EAP in LEW rats. Conversely, LEW rats receiving S-Ag-coupled spleen cells and challenged with IRBP or LEW rats receiving IRBP-coupled spleen cells and challenged with S-Ag developed a severe EAU within 10 days to 2 weeks following immunization, as did all control animals receiving sham-coupled spleen cells and challenged with the two retinal antigens. The results show that the administration of retinal autoantigens coupled to spleen cells effectively protects against the development of EAU when animals are subsequently challenged with the tolerizing antigen but not when challenged with another unrelated pathogenic retinal autoantigen.
Dua
H S
HS
Retina Service, Wills Eye Hospital, Philadelphia, Pennsylvania 19107.
Gregerson
D S
DS
Donoso
L A
LA
D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D019393:Arrestin; D001324:Autoantigens; D001327:Autoimmune Diseases / Q000517:prevention & control*; D005022:Ethyldimethylaminopropyl Carbodiimide; D005136:Eye Proteins / Q000008:administration & dosage*; D005260:Female; D007249:Inflammation / Q000517:prevention & control*; D008214:Lymphocytes; D010870:Pineal Gland / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D012177:Retinol-Binding Proteins / Q000008:administration & dosage* / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D014605:Uveitis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*
D000941:Antigens; D019393:Arrestin; D001324:Autoantigens; D005136:Eye Proteins; D012177:Retinol-Binding Proteins; D005022:Ethyldimethylaminopropyl Carbodiimide