A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals.
A panel of human monoclonal antibody Fab fragments has been generated against the surface glycoprotein gp120 of type 1 human immunodeficiency virus (HIV) by antigen selection from a random combinatorial library expressed on the surface of filamentous phage. The library was prepared from 5 ml of bone marrow from an asymptomatic individual who has been HIV-positive for 6 years. The antibodies have high affinity for antigen (mostly with affinity constants of greater than 10(8) M-1) and notable sequence diversity. Given appropriate donor selection, the methods described should allow the generation of antibodies for the evaluation of passive immunization as a therapy for AIDS.
Burton
D R
DR
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Barbas
C F
CF
Persson
M A
MA
Koenig
S
S
Chanock
R M
RM
Lerner
R A
RA
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000235:genetics; D000936:Antigen-Antibody Complex; D001667:Binding, Competitive; D003001:Cloning, Molecular; D003114:Colony-Forming Units Assay; D004797:Enzyme-Linked Immunosorbent Assay; D015723:Gene Library; D015483:HIV Antibodies / Q000032:analysis* / Q000235:genetics; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D006679:HIV Seropositivity; D015497:HIV-1 / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000032:analysis / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D008214:Lymphocytes / Q000276:immunology; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D012313:RNA