Histamine release from rat mast cells induced by econazole.
1. Econazole released histamine from rat mast cells in vitro. This response was not affected by the addition of calcium or by prior treatment of mast cells with EDTA or cromoglycate. 2. Rat mast cells treated with econazole were stained by the vital dye trypan blue. 3. The intradermal injection of econazole increased vascular permeability. This response was antagonized by chlorpheniramine and cyproheptadine. 4. Our results demonstrate that econazole releases histamine by the "nonselective" mechanism. It is suggested that econazole inflammatory effects may be due to histamine release from mast cells.
Hanada
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S
Department of Pharmacology, Universidade de São Paulo, Brazil.
Oga
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S
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002199:Capillary Permeability / Q000187:drug effects; D002744:Chlorpheniramine / Q000494:pharmacology; D004205:Cromolyn Sodium / Q000494:pharmacology; D003533:Cyproheptadine / Q000494:pharmacology; D004464:Econazole / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014343:Trypan Blue
D002121:Calcium Channel Blockers; D003533:Cyproheptadine; D002744:Chlorpheniramine; D004464:Econazole; D004492:Edetic Acid; D014343:Trypan Blue; D004205:Cromolyn Sodium; D002118:Calcium