title: A class III transcription factor composed of RNA.
abstract: It is generally assumed that the machinery that transcribes genes is composed entirely of polypeptides. However, in vitro transcription by silkworm RNA polymerase III requires a transcription factor that is not a polypeptide. This component, TFIIIR, is distinct from the previously identified transcription components: RNA polymerase III, and the accessory factors TFIIIA, TFIIIB, TFIIIC, and TFIIID. The newly discovered TFIIIR is a macromolecule that appears to be composed of RNA. It is resistant to heat, detergent, phenol, protease, and deoxyribonuclease, but it is sensitive to alkali and ribonuclease.
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene 97403.
authors.forename: L S
authors.initials: LS
authors.lastname: Young
authors.forename: H M
authors.initials: HM
authors.lastname: Dunstan
authors.forename: P R
authors.initials: PR
authors.lastname: Witte
authors.forename: T P
authors.initials: TP
authors.lastname: Smith
authors.forename: S
authors.initials: S
authors.lastname: Ottonello
authors.forename: K U
authors.initials: KU
authors.lastname: Sprague
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics; D007700:Kinetics; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D012320:RNA Polymerase III / Q000378:metabolism; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012344:RNA, Transfer, Ala / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D012341:RNA, Ribosomal, 5S; D012344:RNA, Transfer, Ala; D014157:Transcription Factors; D012313:RNA; D012320:RNA Polymerase III