Merosin promotes cell attachment and neurite outgrowth and is a component of the neurite-promoting factor of RN22 schwannoma cells.
The laminin-like protein merosin was purified from human placenta in intact form and as pepsin fragments and compared to laminin in heparin affinity chromatography and cell binding assays. Intact merosin and a small fragment of merosin comprising the last two repeats of the heavy chain g domain bind to heparin. Intact merosin and large pepsin fragments of merosin, but not the small C-terminal fragment, mediate the attachment and spreading of several types of cells and promote neurite outgrowth from neuronal cells similar to laminin and its corresponding fragments. Cells with various integrin-type receptors for laminin attached equally well to merosin and laminin, suggesting that several of the known laminin binding receptors also bind to merosin. Antibodies to the beta 1 subunit of integrins inhibited neurite outgrowth on merosin as well as on laminin, confirming the involvement of integrin-mediated interaction of cells with both merosin and laminin. Schwannoma cells, which have previously been shown to produce a laminin-like, neurite-promoting factor, synthesize merosin in vivo and in vitro as shown by protein and mRNA analysis. The results suggest that merosin, which is the more abundant basement membrane protein in the laminin family, has properties very similar to laminin despite differences in the structure of the heavy chain. Furthermore, merosin may be identical to or a component of the neurite-promoting factors previously reported from heart, muscle, and Schwann cells.
Engvall
E
E
Cancer Research Center, La Jolla Cancer Research Foundation, California 92037.
Earwicker
D
D
Day
A
A
Muir
D
D
Manthorpe
M
M
Paulsson
M
M
D001483:Base Sequence; D015152:Blotting, Northern; D002448:Cell Adhesion / Q000502:physiology*; D002845:Chromatography; D006801:Humans; D015151:Immunoblotting; D016023:Integrins / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D016023:Integrins; D007797:Laminin; D008565:Membrane Proteins