Flexible protocol for administration of human follicle-stimulating hormone with gonadotropin-releasing hormone antagonist.
OBJECTIVE
To test, using a primate model, a new approach for achieving individualized pituitary-ovarian responses to controlled ovarian hyperstimulation.


METHODS
Normal ovulatory adult monkeys were selected and randomly assigned to one of the three groups according to onset of spontaneous menses. They had no prior exposure to gonadotropin-releasing hormone (GnRH) antagonist or exogenous gonadotropin therapies.


METHODS
The laboratories of The Jones Institute for Reproductive Medicine were used.


METHODS
Normal adult macaque females were studied.


METHODS
Monkeys received hormonal therapies of gonadotropins in combination with GnRH antagonist.


METHODS
Pituitary luteinizing hormone (LH) secretion and ovarian estrogen and progesterone production were monitored.


RESULTS
Adding GnRH antagonist to ongoing human follicle-stimulating hormone (hFSH) stimulation can prevent unwanted LH surges, whether begun at early, mid, or late points in the stimulation protocol.


CONCLUSIONS
The flexible protocol for administration of hFSH with GnRH antagonist yielded satisfactory results, with apparent advantages of economy, convenience, and individuality of treatment compared with GnRH agonist plus gonadotropin regimens used currently.
Byrd
S
S
Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23510.
Itskovitz
J
J
Chillik
C
C
Hodgen
G D
GD
D000818:Animals; D004958:Estradiol / Q000097:blood*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism*; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008597:Menstrual Cycle / Q000097:blood; D010060:Ovulation; D011374:Progesterone / Q000097:blood*
C057805:LHRH, N-Ac-2-Nal(1)-4-Cl-Phe(2)-3-Pal(3)-Arg(5)-Glu(6)-AlaNH2(10)-; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone