title: Isolation and some properties of a 34-kDa-membrane protein that may be responsible for ribosome binding in rat liver rough microsomes.
abstract: We have isolated, by hydroxyapatite chromatography with a non ionic detergent and a high salt concentration, a non-glycosylated, membrane protein with a relative molecular weight of 34 kDa that had previously been found to be a major constituent of the membrane protein fraction showing ribosome-binding activity derived from rat liver rough microsomes (RM). The isolated 34 kDa protein (p34), when incorporated into a liposome model membrane, exhibited significant binding activity toward ribosomes, its binding properties being similar to those observed with intact RM. Immunochemical analyses using antibodies directed against p34 suggested that it is a membrane-embedded RM surface protein, which is specifically localized in ribosome-attached organelles and widely distributed among mammalian tissues. These results would constitute evidence that p34 is a likely candidate for an RM ribosome-binding protein.
authors.affiliation: Department of Biosystem Science, Graduate School of Science and Technology, Niigata University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ichimura
authors.forename: T
authors.initials: T
authors.lastname: Ohsumi
authors.forename: Y
authors.initials: Y
authors.lastname: Shindo
authors.forename: T
authors.initials: T
authors.lastname: Ohwada
authors.forename: H
authors.initials: H
authors.lastname: Yagame
authors.forename: Y
authors.initials: Y
authors.lastname: Momose
authors.forename: S
authors.initials: S
authors.lastname: Omata
authors.forename: H
authors.initials: H
authors.lastname: Sugano
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008081:Liposomes; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D008081:Liposomes; D008565:Membrane Proteins