Retroviral pseudotypes produced by rescue of a Moloney murine leukemia virus vector by C-type, but not D-type, retroviruses.
Human HOS cells containing a Moloney murine leukemia virus (Mo-MLV) recombinant genome were infected by a panel of retroviruses. The C-type viruses simian sarcoma associated virus, feline leukemia virus subgroup B, and the feline endogenous virus RD114 were able to form pseudotypes with the Mo-MLV genome, which transferred a selectable marker gene to target cells; however, Human T cell leukemia virus-1 and the D-type viruses Mason-Pfizer monkey virus and simian retrovirus-1 failed to rescue the Mo-MLV vector. Further characterization of the RD114 pseudotype demonstrated that it retained the receptor specificity of RD114 and will therefore prove useful in receptor characterization.
Takeuchi
Y
Y
Chester Beatty Laboratories, Institute of Cancer Research, London, United Kingdom.
Simpson
G
G
Vile
R G
RG
Weiss
R A
RA
Collins
M K
MK
D000818:Animals; D000914:Antibodies, Viral; D000918:Antibody Specificity; D002460:Cell Line; D005822:Genetic Vectors; D006378:Helper Viruses / Q000235:genetics; D006801:Humans; D008979:Moloney murine leukemia virus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D012190:Retroviridae / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D014779:Virus Replication
D000914:Antibodies, Viral