Immunocytochemical localization of neuromedin K (neurokinin B) in rat spinal ganglia and cord.
Specific antisera directed against substance P and neuromedin K (neurokinin B) have been used in double-label immunofluorescence studies to unambiguously localize these two neuropeptides of the tachykinin family in single tissue sections of rat spinal cord and dorsal root ganglia. Substance P-like immunoreactivity (SPLI) is present but neuromedin K-like immunoreactivity (NMKLI) is undetectable in dorsal root ganglia. Both peptides are present in the spinal cord, but NMKLI is largely restricted to the dorsal gray while SPLI shows a broader distribution. In the spinal gray, NMKLI coexists with SPLI in some, but not all, fibers. While substance P in the dorsal spinal cord is largely of primary afferent origin, neuromedin K appears to originate largely from intrinsic spinal neurons.
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Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.
Maggio
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JE
D000818:Animals; D005260:Female; D005727:Ganglia, Spinal / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D015288:Neurokinin A / Q000378:metabolism; D015287:Neurokinin B / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism; D014018:Tissue Distribution
D013373:Substance P; D015288:Neurokinin A; D015287:Neurokinin B