title: Heparin alters the expression of different forms of immunoglobulin mu heavy chains and their associated proteins by pre-B cell lines and normal Ly-1 (CD5+) B cells.
abstract: Studies presented here show that heparin alters immunoglobulin expression by murine pre-B cell lines and normal Ly-1 (CD5+) B cells. Previous studies have shown that pre-B cell lines 70Z/3 and NFS-5.3 express mu heavy chains in the cytoplasm and a small amount on the cell surface. Both these cytoplasmic and surface mu are disulfide-linked to omega (lambda 5) surrogate light chains and are noncovalently associated with iota (Vpre-B) variable region-like proteins. We show that culturing 70Z/3 with heparin reduces the amount of the membrane-form mu (micron) on the cell surface. Culturing NFS-5.3 with heparin similarly decreases the membrane-form mu; however, it increases the surface level of a pentameric mu molecule containing secreted-form mu (microS) heavy chains, disulfide-linked omega (lambda 5) chains, and noncovalently associated proteins. Culturing peritoneal B cells with heparin also increases the production of the secreted-form microS, detectable in this case by the secretion of classical pentameric IgM. Similarly, injecting heparin intraperitoneally increases IgM secretion by peritoneal Ly-1 B cells. Thus heparin could influence pre-B cell and B cell differentiation and function.
authors.affiliation: Department of Genetics, Stanford University, CA 94305.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: L A
authors.initials: LA
authors.lastname: Herzenberg
authors.forename: L A
authors.initials: LA
authors.lastname: Herzenberg
mesh_terms: D000818:Animals; D015703:Antigens, CD; D000950:Antigens, Ly; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D018956:CD5 Antigens; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000276:immunology; D006493:Heparin / Q000494:pharmacology*; D007147:Immunoglobulin Light Chains; D054444:Immunoglobulin Light Chains, Surrogate; D007075:Immunoglobulin M / Q000235:genetics / Q000378:metabolism; D007148:Immunoglobulin mu-Chains / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000950:Antigens, Ly; D018956:CD5 Antigens; D007147:Immunoglobulin Light Chains; D054444:Immunoglobulin Light Chains, Surrogate; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D008562:Membrane Glycoproteins; D011947:Receptors, Antigen, B-Cell; D006493:Heparin