A method for identifying megakaryocytic and erythroid cells by a combined immunocytochemical and cytochemical stain.
The effectiveness of immunoperoxidase staining for platelet glycoprotein IIb/IIIa coupled with cytochemical staining for hemoglobin has been evaluated as a dual staining technique for identifying lineage-specific characteristics of the megakaryocytic and erythroid series in cells of the same hemopoietic tissue preparation. A sequence of staining was established which produced a circumferential brown stain around cells of the megakaryocytic series, and a diffuse yellow to orange stain in the cytoplasm of cells of the erythroid series. Each staining reaction possessed a high degree of specificity and sensitivity which enabled the combined stain to provide a convenient means for establishing the megakaryocytic and erythroid nature of individual cells in hematological specimens where classification cannot be achieved with certainty on morphological grounds.
Plain
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Department of Medicine, University of Melbourne, St Vincent's Hospital, Fitzroy, Victoria.
Firkin
F C
FC
D002469:Cell Separation / Q000379:methods*; D015672:Erythroid Precursor Cells / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008533:Megakaryocytes / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods