title: Interleukin-8 immunoreactivity in the skin of healthy subjects and patients with palmoplantar pustulosis and psoriasis.
abstract: Previous studies have shown that neutrophil-activating peptide 1/interleukin-8 (IL-8) is present in psoriatic scales and to a lesser extent in normal human epidermis. A panel of monoclonal antibodies and polyclonal antisera raised against IL-8 was used to localize IL-8 with immunoperoxidase techniques in non-lesional and lesional skin of patients with psoriasis and palmo-plantar pustulosis (PPP), and in corresponding sites from healthy subjects. Intracellular IL-8 immunoreactivity was found in all epidermal cell layers in biopsies of healthy subjects and in non-lesional and lesional skin in both PPP and psoriasis. The most intense immunolabeling was regularly found in the basal cell layer. Intercellular epidermal IL-8 immunolabeling was regularly detected in lesional biopsies in PPP and psoriasis, but not in healthy subjects or non-lesional skin in PPP and psoriasis. No intercellular immunolabeling was detected after successful treatment of lesional skin. The majority of cells along the eccrine sweat glands, dermal mononuclear cell infiltrates, and endothelial cells were IL-8 immunoreactive in all biopsies studied. The present study suggests that IL-8, its precursor form, or, alternatively, a degradation product is present in normal human epidermis.
authors.affiliation: Department of Dermatology, Helsinki University Central Hospital, Finland.
authors.forename: H S
authors.initials: HS
authors.lastname: Anttila
authors.forename: S
authors.initials: S
authors.lastname: Reitamo
authors.forename: P
authors.initials: P
authors.lastname: Erkko
authors.forename: M
authors.initials: M
authors.lastname: Ceska
authors.forename: B
authors.initials: B
authors.lastname: Moser
authors.forename: M
authors.initials: M
authors.lastname: Baggiolini
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000032:analysis* / Q000276:immunology; D011565:Psoriasis / Q000378:metabolism*; D012867:Skin / Q000737:chemistry*; D013545:Sweat Glands / Q000737:chemistry
chemical_list: D016209:Interleukin-8