Sarcocystis muris (Apicomplexa): a thiol protease from the dense granules.
Homogenates of Sarcocystis muris merozoites (cyst form) and the subcellular fraction of dense granules were assayed for protease activity with substrate-impregnated SDS-polyacrylamide gels. Four acidic and several basic proteases were detected in the merozoites. One of the basic proteases was further characterized as a thiol protease (EC 3.4.22). The activity of this protease was enriched in the dense granule fraction.
Strobel
J G
JG
Zoologisches Institut, Universität Bonn, Federal Republic of Germany.
Delplace
P
P
Dubremetz
J F
JF
Entzeroth
R
R
D000818:Animals; D002458:Cell Fractionation; D003546:Cysteine Endopeptidases / Q000302:isolation & purification*; D004589:Electrophoresis, Disc; D015388:Organelles / Q000201:enzymology*; D012522:Sarcocystis / Q000201:enzymology* / Q000648:ultrastructure
D003546:Cysteine Endopeptidases