Bacteria-induced histamine release from human bronchoalveolar cells and blood leukocytes.
Histamine release induced by Staphylococcus aureus was examined in cells obtained by bronchoalveolar lavage (BAL) in non-atopic individuals. Approximately half of the individuals responded with mediator release to the bacterium, and the release was found to be time- and concentration dependent. No difference was found between the patients who responded and those who did not respond in regard to age, sex, smoker/non-smoker, % recovery of BAL-fluid, total cell count, differential cell counts, histamine content per mast cell, or diagnoses. Also stimulation of the BAL-cells with the calcium-ionophore A23187 resulted in histamine release. S. aureus-induced histamine release from basophils was examined in leukocyte suspensions obtained from the same individuals, and in all experiments release was found. The dose-response curves were similar to those obtained with BAL cells. The bacteria-induced mediator release from superficially lying cells in the airways epithelium might be of importance for the precipitation or exacerbation of bronchial asthma in respiratory tract infections.
Clementsen
P
P
Department of Pharmacology, University of Copenhagen, Denmark.
Milman
N
N
Struve-Christensen
E
E
Petersen
B N
BN
Pedersen
M
M
Bisgaard
H
H
Permin
H
H
Norn
S
S
D000328:Adult; D000368:Aged; D001249:Asthma / Q000276:immunology / Q000382:microbiology; D001491:Basophils / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology* / Q000378:metabolism; D002452:Cell Count; D005260:Female; D006636:Histamine Release; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008407:Mast Cells; D008875:Middle Aged; D013203:Staphylococcal Infections / Q000276:immunology; D013211:Staphylococcus aureus / Q000502:physiology*; D013997:Time Factors