Antisense oligonucleotides from the stage-specific myeloid zinc finger gene MZF-1 inhibit granulopoiesis in vitro.
Zinc finger proteins are transcriptional regulators of other genes, often controlling developmental cascades of gene expression. A recently cloned zinc finger gene, MZF-1, was found to be preferentially expressed in myeloid cells. Using complementary radiolabeled MZF-1 RNA hybridized to human bone marrow smears in situ, it was discovered that the expression of MZF-1 is essentially limited to the myelocyte and metamyelocyte stages of granulopoiesis. Antisense but not sense oligonucleotides from MZF-1 significantly inhibited granulocyte colony-stimulating factor-driven granulocyte colony formation in vitro.
Bavisotto
L
L
Department of Medicine, University of Washington, Seattle 98195.
Kaushansky
K
K
Lin
N
N
Hromas
R
R
D000328:Adult; D001853:Bone Marrow / Q000502:physiology; D003854:Deoxyribonucleotides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000187:drug effects*; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012313:RNA / Q000235:genetics; D018075:RNA, Complementary; D016335:Zinc Fingers / Q000235:genetics*
D003854:Deoxyribonucleotides; D016376:Oligonucleotides, Antisense; D018075:RNA, Complementary; D012313:RNA