[Thrombogenicity of prothrombin complex concentrates].
PCC's were isolated either by adsorption to DEAE-Sephadex A-50 (particle size 40-120 microns; Pharmacia Uppsala) or by adsorption to Molselect DEAE-50 (particle size 100-320 microns; Reanal Budapest) from human citrated plasma after separation of factor VIII by cryoprecipitation. The two products obtained (without heparin) were both compared by in vitro (NAPTT, TGt50) and in vivo (venous stasis) model in rats acc. to WESSLER) tests. The agent prepared by adsorption to DEAE-Sephadex A-50 showed a significantly higher thrombogenicity than that prepared by Molselect DEAE-50. ED50 values for thrombus formation to amounted 15 Factor IX U/kg for the first product and to 210 factor IX U/kg for the second.
Dornheim
G
G
Bezirksinstitut für Spende- und Transfusionswesen Suhl.
Klöcking
H P
HP
Wulkow
R
R
Storch
H
H
Töpfer
G
G
D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000302:isolation & purification / Q000502:physiology*; D002852:Chromatography, Ion Exchange; D005169:Factor VIII / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007700:Kinetics
D001779:Blood Coagulation Factors; C025667:prothrombin complex concentrates; D005169:Factor VIII
Zur Thrombogenität von Prothrombinkomplexkonzentraten.