Spontaneous and clastogen induced chromosomal breakage in scleroderma.
Spontaneous and clastogen induced chromosomal damage was evaluated in lymphocytes of 10 patients with scleroderma and 21 controls. Peripheral blood lymphocytes from patients with scleroderma not only had elevated rates of spontaneous chromosomal damage (p = 0.002), but also bleomycin induced (p = 0.041), streptonigrin induced (p = 0.035), and 4-nitroquinoline-1-oxide induced (p = 0.032) chromosomal breakage. Our findings suggest that scleroderma lymphocytes may have a generalized susceptibility to DNA damage caused by free radicals.
Wolff
D J
DJ
Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore.
Needleman
B W
BW
Wasserman
S S
SS
Schwartz
S
S
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000328:Adult; D000368:Aged; D001761:Bleomycin / Q000494:pharmacology; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced*; D025063:Chromosome Disorders; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure; D004249:DNA Damage; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D008937:Mitomycins / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology*; D010396:Penicillamine / Q000009:adverse effects; D012595:Scleroderma, Systemic / Q000235:genetics* / Q000473:pathology; D013308:Streptonigrin / Q000494:pharmacology
D008937:Mitomycins; D009153:Mutagens; D001761:Bleomycin; D013308:Streptonigrin; D015112:4-Nitroquinoline-1-oxide; D010396:Penicillamine