title: Molecular forms of acetylcholinesterase in cerebral cortex and dorsal thalamus of developing rats.
abstract: Histochemical studies show that primary sensory regions of rat cerebral cortex and dorsal thalamus display transient patterns of intense acetylcholinesterase (AChE) activity during early postnatal development. Sucrose gradient fractionation techniques were used to determine the molecular forms of AChE in developing rat brain at the time of onset (postnatal day 5), during peak expression (days 10-11), and after decline (day 18) of the transient AChE expression. Tissue from auditory and visual regions of cortex and from dorsal thalamus at each age examined contained 10S and 4S forms of AChE, similar to the pattern observed in mature brain. The 10S form was almost totally membrane bound; the 4S form was largely soluble. Hemithalamic lesions reduce both forms of AChE in cortex. These data indicate that transiently expressed AChE does not represent a unique or unusual form of the enzyme.
authors.affiliation: Department of Psychiatry, V.A. Medical Center, Long Beach, CA 90822.
authors.forename: C
authors.initials: C
authors.lastname: Gorenstein
authors.forename: K A
authors.initials: KA
authors.lastname: Gallardo
authors.forename: R T
authors.initials: RT
authors.lastname: Robertson
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D000818:Animals; D001303:Auditory Cortex / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000201:enzymology* / Q000254:growth & development; D004027:Diencephalon / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D006651:Histocytochemistry; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D013788:Thalamus / Q000201:enzymology* / Q000254:growth & development; D014793:Visual Cortex / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism
chemical_list: D000110:Acetylcholinesterase