Evolutionary conservation of an epitope associated with striated rootlets in different epithelial ciliated cells.
In ciliated cells of metazoa, striated rootlets associated with basal bodies anchor the ciliary apparatus to the cytoskeleton. We have used here a monoclonal antibody against a 175 kDa protein associated with the striated rootlets of quail ciliated cells, to study ciliated cells of different species. In mussel gill epithelium the antibody recognized a protein of 92 kDa which shows a periodic distribution along the striated rootlets. In frog ciliated palate epithelium, two different rootlets are associated with basal bodies, both are decorated and only one protein of 48 kDa is recognized on immunoblot. The antigen is arranged in a helix around the striated rootlets. In rabbit ciliated oviduct epithelium, we detected the presence of very small and thin rootlets which are weakly labeled. We have shown that an epitope associated with the striated rootlets is preserved through evolution although the molecular weight of the peptide varies. We have also observed the appearance of this epitope on protein associated with junctional complexes in rabbit and cytoskeleton component in quail oviduct.
Lemullois
M
M
Centre de Biologie Cellulaire CNRS, Ivry-sur-Seine, France.
Klotz
C
C
Sandoz
D
D
D000818:Animals; D005075:Biological Evolution; D015153:Blotting, Western; D002923:Cilia / Q000276:immunology*; D004848:Epithelium / Q000276:immunology / Q000648:ultrastructure*; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron
D000939:Epitopes