[DNA cytometry in gastric cancer--a comparison between static cytometry on cytologic and histologic preparations and flow cytometry].
Static cytometry on cytologic and histologic preparations and flow cytometry are the basic techniques of DNA cytometry with certain advantages and drawbacks. An exact and reliable assessment of DNA ploidy and stem line heterogeneity of paraffin-embedded specimens is best performed with static cytometry on smears. The high resolution of DNA histograms in flow cytometry is reduced by the often strong portion of detritus and therefore the internal reference peak or small tumor stem lines might not be identified. The use of histologic preparations is advantageous for the analysis of correlations between histomorphologic tumor heterogeneity and stem line heterogeneity.
Mellin
W
W
Gerhard-Domagk-Institut für Pathologie, Universität Münster.
Olf
C
C
Böcker
W
W
D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods; D006652:Histological Techniques; D006801:Humans; D011003:Ploidies; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*
D004273:DNA, Neoplasm
DNA-Zytophotometrie am Magenkarzinom--Ein Vergleich zwischen der statischen Zytophotometrie an Schnittpräparaten und Ausstrichpräparaten und der Flusszytophotometrie.