A new visualization principle of cerium phosphate reaction product of phosphatases in cryotome sections for light microscopy--the cerium-oxalate-osmium-silver (Ce-Ox-Os-Ag) method.
A new visualization principle for the detection of cerium phosphate reaction product of phosphatases in light microscopy is described. The new mode is based on the conversion of cerium phosphate into cerium oxalate. The latter is able to react with OsO4. In this way osmium black is formed, staining enzymatic activity sites grey or greyish-black. Utilizing the argyrophilia of osmium black, the staining contrast could be remarkably intensified by posttreatment with Ag-proteinate (Ce-Ox-Os-Ag procedure). This procedure is of similar sensitiveness in comparison to the DAB-Ni method, proposed earlier. The advantages are a very pale background staining and a complete suppression of the cerophilia. Moreover, it substitutes to DAB, a probable potent carcinogen. The method is time-saving when the processing of the reactions was stimulated in a microwave oven.
Halbhuber
K J
KJ
Department of Histochemistry, Friedrich Schiller University, Jena, Germany.
Feuerstein
H
H
D000818:Animals; D002563:Cerium / Q000737:chemistry*; D005260:Female; D006651:Histocytochemistry; D008297:Male; D009993:Osmium Tetroxide / Q000737:chemistry; D010070:Oxalates / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012680:Sensitivity and Specificity; D012836:Silver Proteins / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
D010070:Oxalates; D010710:Phosphates; D012836:Silver Proteins; D002563:Cerium; D010744:Phosphoric Monoester Hydrolases; D009993:Osmium Tetroxide; C048083:cerium phosphate