In vivo tumor growth enhancement by granulocyte colony-stimulating factor.
The intraperitoneal administration of human recombinant granulocyte colony-stimulating factor (G-CSF) enhanced the growth of intradermally inoculated tumor in mice; in a Meth A fibrosarcoma model, G-CSF administration significantly shortened the latency before tumor appearance, accelerated the increase of tumor size, shortened the survival time of tumor-bearing mice and increased the incidence of lethal tumor growth. A similar growth-enhancing effect of G-CSF was observed in models employing Meth 1 fibrosarcoma, colon carcinoma 26, and L1210 leukemia, although not all the effects were statistically significant. In vitro study showed that G-CSF did not enhance Meth A growth in suspension culture or in soft agar. These data suggest that G-CSF enhances the Meth A growth not directly but through the mediation of host factors. The accumulation of neutrophils was histologically observed in the tumor nodule, the blood, and the spleen in mice given G-CSF repeatedly. The spleen cells and the peripheral blood leukocytes of G-CSF-injected mice enhanced Meth A growth in vitro as compared with those of mice injected with physiological saline. These results suggest the possibility that the in vivo growth of tumor cells was enhanced by G-CSF-induced overproduction of cells including neutrophils.
Segawa
K
K
Division of Experimental Chemotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo.
Ueno
Y
Y
Kataoka
T
T
D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000097:blood / Q000503:physiopathology*; D005260:Female; D005354:Fibrosarcoma / Q000097:blood / Q000503:physiopathology*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000503:physiopathology*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009504:Neutrophils; D012513:Sarcoma, Experimental / Q000097:blood / Q000503:physiopathology*; D013154:Spleen / Q000166:cytology
D016179:Granulocyte Colony-Stimulating Factor