title: Induction of nitrite production in mouse spleen cells by immunization.
abstract: Changes of nitrite production in mouse spleen cells of in vitro secondary antibody response were investigated. Mouse spleen cells immunized with gamma globulin fraction of rat serum produced nitrite 3 days after in vitro challenging with the same antigen. Nitrite production of rabbit IgG-challenged spleen cells was found to be about 2.9-times higher than that of spleen cells primed with the gamma globulin fraction of rat serum. Nitrite production in this system was completely suppressed by T cell depletion (99.7% inhibition). Furthermore, nitrite production in these cells significantly decreased by addition of anti-interferon gamma antibody (62.9% inhibition). These data indicate that nitrite production in antigen-immunized spleen cells is affected with the immunogenicity of an antigen and regulated by T cells, especially interferon (IFN) gamma.
authors.affiliation: Department of Environment Biochemistry, School of Pharmaceutical Science, University of Shizuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takagi
authors.forename: H
authors.initials: H
authors.lastname: Nakagami
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: Y
authors.initials: Y
authors.lastname: Suketa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002478:Cells, Cultured; D007114:Immunization; D007074:Immunoglobulin G / Q000378:metabolism / Q000494:pharmacology; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008212:Lymphocyte Depletion; D051379:Mice; D009573:Nitrites / Q000378:metabolism*; D013154:Spleen / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D005719:gamma-Globulins / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009573:Nitrites; D005719:gamma-Globulins; D007371:Interferon-gamma