title: Leukocyte biophysics. An invited review.
abstract: The biophysical properties of leukocytes in the passive and active state are discussed. In the passive unstressed state, leukocytes are spherical with numerous membrane folds. Passive leukocytes exhibit viscoelastic properties, and the stress is carried largely by the cell cytoplasm and the nucleus. The membrane is highly deformable in shearing and bending, but resists area expansion. Membrane tension can usually be neglected but plays a role in cases of large deformation when the membrane becomes unfolded. The constant membrane area constraint is a determinant of phagocytic capacity, spreading of cells, and passage through narrow pores. In the active state, leukocytes undergo large internal cytoplasmic deformation, pseudopod projection, and granule redistribution. Several different measurements for assessment of biophysical properties and the internal cytoplasmic deformation in form of strain and strain rate tensors are presented. The current theoretical models for active cytoplasmic motion in leukocytes are discussed in terms of specific macromolecular reactions.
authors.affiliation: Department of AMES-Bioengineering, University of California, San Diego, La Jolla 92093.
authors.forename: G W
authors.initials: GW
authors.lastname: Schmid-Schönbein
mesh_terms: D055592:Biophysical Phenomena; D001703:Biophysics; D003593:Cytoplasm / Q000648:ultrastructure; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D008433:Mathematics; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning