title: New monoclonal antibodies reacting with bile ducts: further insights into the pathogenesis of bile ductular proliferation in biliary diseases.
abstract: We have produced a range of monoclonal antibodies which stain human intrahepatic bile ducts of different sizes. Amongst 26 monoclonal antibodies produced, five clones reacted specifically with bile ducts of different sizes, of which three have been maintained in culture and their viability following freezing and thawing confirmed. Staining patterns varied between normal adult liver tissue, normal fetal liver tissue and a variety of hepatobiliary diseases. The antibodies provide further evidence of the immunological heterogeneity of the human intrahepatic biliary tree and support the hypothesis that proliferating bile ductules are derived from periseptal hepatocytes. The preparation of the antibodies, their staining reactions in normal adult, normal fetal and a variety of liver diseases are described.
authors.affiliation: Liver Unit, Queen Elizabeth Hospital, Birmingham, U.K.
authors.forename: R C
authors.initials: RC
authors.lastname: Ayres
authors.forename: S G
authors.initials: SG
authors.lastname: Hübscher
authors.forename: J
authors.initials: J
authors.lastname: Shaw
authors.forename: C
authors.initials: C
authors.lastname: Garner
authors.forename: R
authors.initials: R
authors.lastname: Joplin
authors.forename: A
authors.initials: A
authors.lastname: Williams
authors.forename: J M
authors.initials: JM
authors.lastname: Neuberger
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D001649:Bile Duct Diseases / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic / Q000473:pathology*; D002761:Cholangitis / Q000473:pathology; D005333:Fetus; D006801:Humans; D008099:Liver / Q000473:pathology; D008105:Liver Cirrhosis, Biliary / Q000473:pathology; D008938:Mitosis; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal