Role of antigen-binding B lymphocytes in the formation of antigen-dependent nonspecific immunoglobulin-forming cells.
A role for antigen-binding B cells in the induction of antigen-dependent B cells producing antigen-nonspecific immunoglobulins (nIFC) is analyzed. An in vitro immune response system was used; sheep red blood cells were employed as the antigen. It is shown that cocultivation of antigen-binding B cells with resting naive splenocytes in the presence of homologous antigen resulted not only in an increase in the number of antibody-forming cells (AFC), but also in a sharp increase in nIFC. Cocultivation of G0 splenocytes with primed B cells depleted for antigen-binding cells did not affect AFC induction, and only slightly augmented the number of nIFC. It is suggested that some of the nIFC appearing after antigenic stimulation arise from resting B lymphocytes in response (direct or indirect) to nonspecific B-cell factor(s).
Agadjanyan
M G
MG
Institute of Viral Preparations, Academy of Medical Sciences of the USSR, Moscow.
Sidorova
E V
EV
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000378:metabolism / Q000502:physiology*; D000941:Antigens / Q000276:immunology*; D016175:B-Lymphocyte Subsets / Q000166:cytology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D000939:Epitopes; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D012756:Sheep; D013154:Spleen
D000941:Antigens; D000939:Epitopes