title: [A bioluminescent method of determining the sensitivity of microflora to antibiotics].
abstract: A bioluminescent rapid method (a three-hour test) has been developed to assess the susceptibility of microflora to antibiotics. It is based on comparison of intracellular ATP content in a sample (pus) after 3 h incubation at 37 degrees C in liquid culture medium (dilution: sample/culture medium--1:9) on a shaker in the presence and absence (control) of a single antibiotic concentration. A criterion has been offered for quantitative estimation of antibiotic susceptibility of microorganisms. The authors have optimized the measurements of intracellular ATP by the rapid bioluminescent method using immobilized firefly luciferase-based ATP reagent. The results of the method are in good correlation with those of the standard agar diffusion technique.
authors.forename: A I
authors.initials: AI
authors.lastname: Titov
authors.forename: N A
authors.initials: NA
authors.lastname: Romanova
authors.forename: T M
authors.initials: TM
authors.lastname: Danilova
authors.forename: L Iu
authors.initials: LIu
authors.lastname: Brovko
authors.forename: N N
authors.initials: NN
authors.lastname: Ugarova
authors.forename: P I
authors.initials: PI
authors.lastname: Tolstykh
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests / Q000379:methods*
chemical_list: D000255:Adenosine Triphosphate
vernacular_title: Bioliuminestsentnyĭ metod opredeleniia chuvstvitel'nosti mikroflory k antibiotikam.