title: Altered p53 gene structure and expression in human epithelial cells after exposure to nickel.
abstract: The carcinogenicity of certain nickel compounds is well known. We have previously shown that human kidney epithelial cells were immortalized by treatment with Ni(II) and in cooperation with the v-Ha-ras oncogene transformed the cells to acquire tumorigenicity in athymic nude mice. Immunocytochemistry and sequence analysis of DNA from the nickel-immortalized cells revealed abnormal p53 expression and a T----C transition mutation in codon 238. These data are consistent with the hypothesis that Ni(II)-induced mutation in the p53 gene can be involved in the escape from senescence of kidney epithelial cells.
authors.affiliation: Department of Toxicology, National Institute of Occupational Health, Oslo, Norway.
authors.forename: L
authors.initials: L
authors.lastname: Maehle
authors.forename: R A
authors.initials: RA
authors.lastname: Metcalf
authors.forename: D
authors.initials: D
authors.lastname: Ryberg
authors.forename: W P
authors.initials: WP
authors.lastname: Bennett
authors.forename: C C
authors.initials: CC
authors.lastname: Harris
authors.forename: A
authors.initials: A
authors.lastname: Haugen
mesh_terms: D002461:Cell Line, Transformed; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17; D004848:Epithelium / Q000187:drug effects; D016158:Genes, p53 / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000187:drug effects*; D009154:Mutation / Q000235:genetics*; D009532:Nickel / Q000633:toxicity*
chemical_list: D009532:Nickel