title: Aflatoxin B1-DNA adducts and hepatitis B virus antigens in hepatocellular carcinoma and non-tumorous liver tissue.
abstract: Studies were carried out to test the hypothesis that exposure to aflatoxin B1 (AFB1) is common among individuals with hepatocellular carcinoma (HCC) who are also chronically infected with hepatitis B virus (HBV). Experiments were also carried out to determine whether there is a close association between the presence of AFB1-DNA adducts and the expression of one or more HBV antigens in the tumor or non-tumor regions of the liver. Twenty-seven paired tumor and non-tumor liver tissues of HCC patients from Taiwan were analyzed. Monoclonal antibody 6A10, generated against the imidazole ring-opened persistent form of the major N-7 guanine adduct of AFB1, was used for adduct detection by both indirect immunofluorescence and competitive enzyme-linked immunosorbent assay. An avidin-biotin complex staining method was used for the detection of HBsAg and HBxAg in liver sections. A total of 8 (30%) HCC samples and 7 (26%) adjacent non-tumor liver tissue samples from Taiwan were positive for AFB1-DNA adducts. For HBsAg, 10 (37%) HCC samples and 22 (81%) adjacent non-tumorous liver samples were positive while 9 (33%) HCC samples and 11 (41%) adjacent non-tumor liver samples were HBxAg-positive. No association with AFB1-DNA adducts was observed for HBsAg and HBxAg. These results suggest that both AFB1 exposure and carrier status of HBsAg/HBxAg may be involved in the induction of HCC in Taiwan.
authors.affiliation: Comprehensive Cancer Center, School of Public Health, Columbia University, New York, NY 10032.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Zhang
authors.forename: C J
authors.initials: CJ
authors.lastname: Chen
authors.forename: C S
authors.initials: CS
authors.lastname: Lee
authors.forename: B
authors.initials: B
authors.lastname: Haghighi
authors.forename: G Y
authors.initials: GY
authors.lastname: Yang
authors.forename: L W
authors.initials: LW
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Feitelson
authors.forename: R
authors.initials: R
authors.lastname: Santella
mesh_terms: D016604:Aflatoxin B1 / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000737:chemistry* / Q000276:immunology; D004247:DNA / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006511:Hepatitis B Antigens / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000737:chemistry* / Q000276:immunology; D008113:Liver Neoplasms / Q000737:chemistry* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D013194:Staining and Labeling; D015534:Trans-Activators / Q000032:analysis; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D006511:Hepatitis B Antigens; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D004247:DNA; D016604:Aflatoxin B1