title: Allo-cross-reactivity of a human neuraminidase-specific T cell clone dependent on presentation of an endogenous B cell-specific antigen.
abstract: T cells specific for foreign antigen recognize a complex of peptides and self-major histocompatibility complex (MHC) molecules and can also cross-react with allo-MHC molecules. It remains controversial, however, what alloreactive T cells exactly recognize. It has been proposed that alloreactive T cells recognize endogenous peptides presented by allo-MHC molecules. To test this hypothesis, we examined an influenza virus-specific T cell clone (6H5), specific for neuraminidase N2 and restricted by HLA-DR1. In the absence of influenza virus, this clone cross-reacted with HLA-DR1Dw1+ but not with HLA-DR1Dw20+ Epstein-Barr virus-transformed lymphoblastoid cells (B-LCL). Cold target inhibition experiments and the rearrangement pattern of the T cell receptor beta chain indicated that 6H5 was a monoclonal T cell population most likely using the same T cell receptor for both responses. To determine whether determinants other than HLA-DR1Dw1+ B-LCL or activated B cells, but, surprisingly, not to other cell types expressed HLA-DR1Dw1, including monocytes and transfected L cells. These experiments further support the concept that recognition of allogeneic MHC (in this case HLA-DR1Dw1) may result from a cross-reactivity of T cells specific for a complex of foreign antigen and self-MHC (neuraminidase N2 and HLA-DR1Dw20). Furthermore, allorecognition of T cell clone 6H5 appears to depend upon the recognition of a complex of allogeneic MHC and a cell-type specific endogenous peptide presented by activated B cells.
authors.affiliation: Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: K C
authors.initials: KC
authors.lastname: Kuijpers
authors.forename: P
authors.initials: P
authors.lastname: Treep-Van Leeuwen
authors.forename: F
authors.initials: F
authors.lastname: Miedema
authors.forename: C J
authors.initials: CJ
authors.lastname: Lucas
mesh_terms: D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules / Q000032:analysis; D002999:Clone Cells; D003429:Cross Reactions; D000939:Epitopes; D006684:HLA-DR Antigens / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D008562:Membrane Glycoproteins / Q000032:analysis; D009439:Neuraminidase / Q000276:immunology*; D009975:Orthomyxoviridae / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules; D000939:Epitopes; D006684:HLA-DR Antigens; D008562:Membrane Glycoproteins; D018799:Intercellular Adhesion Molecule-1; D009439:Neuraminidase