Sequence complexity of polyadenylated RNA from seizure-susceptible El mouse brain polysomes.
The hybridization kinetics in the complementary DNAs (cDNA) to polyadenylated messenger RNA (mRNA) in the brain of seizure-susceptible El mice were examined to elucidate the correlation between seizure susceptibility and genetic expression. Homologous and heterologous saturation hybridizations indicated that cDNA of seizure-nonsusceptible ddY mice hybridized with less mRNA in seizure-experienced El(+) or seizure-nonexperienced El(o) mice than that in ddY mice, cDNA of El(+) mice hybridized to a lesser extent with mRNA of El(o) mice than that of ddY or El(+) mice, and no difference was observed with cDNA hybridization results of El(o) mice to individual mRNAs of ddY, El(+) or El(o) mice. The results suggest that some sequences found in mRNA of ddY are lacking in both El strains and are responsible for the inbred predisposition, while those present in El(+) mRNA but missing in El(o) may associate with seizure susceptibility, since common sequences are present in the mRNA of those groups of animal. The sequence diversity among the 3 groups of mice was mainly observed with the rare class of mRNA. Molecular cloning of the cDNA corresponding to this class of mRNA found in the El(+) mice would clarify the gene expression in the brains of epileptics.
Mui
K
K
Department of Neuropsychiatry, Osaka City University Medical School, Japan.
Yamagami
S
S
Kioka
T
T
Onishi
H
H
Kawakita
Y
Y
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004587:Electrophoresis, Agar Gel; D004827:Epilepsy / Q000235:genetics*; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics* / Q000302:isolation & purification; D011132:Polyribosomes / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*
D012333:RNA, Messenger; D011061:Poly A; D004247:DNA; D012194:RNA-Directed DNA Polymerase