title: Experimental autoimmune uveoretinitis and pinealitis induced by interphotoreceptor retinoid-binding protein and S-antigen: induction of intraretinal and subretinal neovascularization.
abstract: Experimental autoimmune uveoretinitis (EAU) and pinealitis were induced in Lewis rats following hind foot pad injection of interphotoreceptor retinoid-binding protein (IRBP) or S-antigen. A comparison is made in this study of the in vivo and histological changes in uveoretinitis and pinealitis induced by administering similar doses of highly-purified IRBP and S-antigen emulsified in complete Freund's adjuvant (CFA). The time of onset of ocular inflammation after inoculation was slightly later in S-antigen (14-18 days) as compared with IRBP-inoculated animals (10-14 days), while the severity of the inflammation was lower in the latter group. The distribution of inflammation in the anterior segment was similar in both the S-antigen and IRBP sensitized animals but there was major variation in the location of the posterior segment disease. Vasculitis was a predominant feature of IRBP induced disease while chorioretinitis and photoreceptor destruction was more prominent in the S-antigen sensitized animals. A striking feature of this study is that both antigens induced intraretinal and subretinal neovascularization, an observation which has not been reported previously. Inflammation was induced in all pineal glands and as with EAU the severity was closely related to the type of antigen inoculated.
authors.affiliation: Department of Pharmacy, University of Strathclyde, UK.
authors.forename: S
authors.initials: S
authors.lastname: al-Mahdawi
authors.forename: P M
authors.initials: PM
authors.lastname: McGettrick
authors.forename: W R
authors.initials: WR
authors.lastname: Lee
authors.forename: D I
authors.initials: DI
authors.lastname: Graham
authors.forename: A
authors.initials: A
authors.lastname: Shallal
authors.forename: C A
authors.initials: CA
authors.lastname: Converse
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology / Q000633:toxicity*; D019393:Arrestin; D001327:Autoimmune Diseases / Q000209:etiology* / Q000276:immunology; D002417:Cattle; D005136:Eye Proteins / Q000276:immunology / Q000633:toxicity*; D007249:Inflammation; D008297:Male; D009389:Neovascularization, Pathologic; D010870:Pineal Gland / Q000276:immunology / Q000473:pathology*; D051381:Rats; D011917:Rats, Inbred Lew; D012177:Retinol-Binding Proteins / Q000276:immunology / Q000633:toxicity*; D015866:Uveitis, Posterior / Q000209:etiology / Q000276:immunology*; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial