Sister-chromatid exchange in human B- and T-lymphocytes exposed to bleomycin, cyclophosphamide, and ethyl methanesulfonate.
Sister-chromatid exchange (SCE) frequencies were investigated in mitogen-stimulated cultures of highly purified human peripheral blood B- and T-lymphocytes exposed to bleomycin (BM), cyclophosphamide (CP), or ethyl methanesulfonate (EMS). In untreated controls, T-lymphocytes showed twice as many SCEs as B-lymphocytes. CP (with metabolic activation) and EMS significantly increased the SCE frequencies. EMS induced a similar, dose-dependent SCE increase in both cell populations, whereas CP induced more SCEs in T- than in B-lymphocytes. No clear SCE increase was found in B- and T-lymphocytes treated with BM.
Miller
K
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Abteilung Humangenetik, Medizinsichen Hochschule Hannover, F.R.G.
D001402:B-Lymphocytes / Q000187:drug effects*; D001711:Biotransformation; D001761:Bleomycin / Q000633:toxicity*; D002455:Cell Division; D003520:Cyclophosphamide / Q000493:pharmacokinetics / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D006801:Humans; D007700:Kinetics; D012854:Sister Chromatid Exchange; D013601:T-Lymphocytes / Q000187:drug effects*
D001761:Bleomycin; D003520:Cyclophosphamide; D005020:Ethyl Methanesulfonate