title: Interaction of HIV-1 with susceptible lymphoblastoid cells. 1H NMR studies.
abstract: Different strains of HIV susceptible lymphoblastoid cells have been infected by HIV-1 and examined by means of 1H NMR spectroscopy at different times after infection, taking advantage of the presence of high resolution lipid signals from the plasma membrane of tumor cells. A transient decrease in intensity of fatty acid signals, originated by changes in membrane structure, has been observed early after viral infection. Marked alterations in membrane-dependent steps of phospholipid synthesis can also be inferred by the observed transient depression in peaks from choline-based metabolites. Spectral modifications deriving from changes in lipid metabolism are also produced both in infected cells a few days after infection and in permanently infected cells. 1H NMR can, therefore, monitor structural and metabolic effects induced by HIV infection.
authors.affiliation: Laboratorio di Fisica e INFN Sez. Sanita, Roma, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Luciani
authors.forename: A
authors.initials: A
authors.lastname: Rosi
authors.forename: M T
authors.initials: MT
authors.lastname: Maggiorella
authors.forename: M
authors.initials: M
authors.lastname: Federico
authors.forename: N
authors.initials: N
authors.lastname: Sulli
authors.forename: P
authors.initials: P
authors.lastname: Verani
authors.forename: G B
authors.initials: GB
authors.lastname: Rossi
authors.forename: V
authors.initials: V
authors.lastname: Viti
authors.forename: L
authors.initials: L
authors.lastname: Guidoni
mesh_terms: D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D005227:Fatty Acids / Q000737:chemistry; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism; D009000:Monocytes / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D005227:Fatty Acids; D008563:Membrane Lipids; D012194:RNA-Directed DNA Polymerase