Fluorescent studies directed towards the location of abnormal epithelial cells in cervical smears.
Cervical screening is concerned with the search for abnormal epithelial cells in smears prepared from scrapings from the uterine cervix. It is a highly skilled labour intensive operation and automated methods of detecting dyskariotic cells in cervical smears would be helpful. We report a fluorescence method of detecting abnormal cervical cells in smears and biopsies using a probe for guanidinobenzoatase. This approach has the potential for automation.
Steven
F S
FS
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester.
Johnson
J
J
D000585:Aminacrine; D001331:Automation; D015415:Biomarkers; D001706:Biopsy; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D002278:Carcinoma in Situ / Q000175:diagnosis / Q000473:pathology; D002454:Cell Differentiation; D002584:Cervix Uteri / Q000201:enzymology / Q000473:pathology*; D010450:Endopeptidases; D004848:Epithelium / Q000201:enzymology / Q000473:pathology; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D008856:Microscopy, Fluorescence; D011419:Propidium; D013194:Staining and Labeling / Q000379:methods; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000473:pathology; D014626:Vaginal Smears / Q000379:methods*
D015415:Biomarkers; D008565:Membrane Proteins; D011419:Propidium; D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases