Production of high purity proteins by elution of individual fractions from two-dimensional gels.
Results are presented on the production of purified myocardial proteins from two-dimensional (2-D) gels. Proteins were fixed in a native condition using potassium acetate and then eluted into an aqueous solution. Homogeneous tropomyosin and myosin light chain fractions and a number of nonidentified myocardial proteins present on 2-D gels were obtained.
Tsvetkova
M N
MN
National Research Center of Medical Genetics, Academy of Medical Sciences, USSR, Moscow.
Kovalyov
L I
LI
Leptev
A V
AV
Shishkin
S S
SS
D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis*; D009206:Myocardium / Q000737:chemistry*; D009218:Myosins / Q000032:analysis; D013194:Staining and Labeling; D014335:Tropomyosin / Q000032:analysis
D009124:Muscle Proteins; D014335:Tropomyosin; D009218:Myosins