Replication of the R6K plasmid during the Escherichia coli cell cycle.
The cell-cycle replication pattern of the R6K plasmid has been investigated by using the membrane-elution technique to produce cells labelled at different times during the division cycle and scintillation counting for quantitative analysis of radioactive plasmid DNA. The high-copy plasmid R6K replicates exponentially in a cell-cycle-independent manner. A mini-R6K plasmid deleted for the ori alpha origin of replication also replicates, exponentially in a cell-cycle-independent manner.
Keasling
J D
JD
Department of Chemical Engineering, University of Michigan, Ann Arbor 48109-2136.
Palsson
B O
BO
Cooper
S
S
D002453:Cell Cycle / Q000502:physiology*; D004261:DNA Replication; D004926:Escherichia coli / Q000502:physiology*; D010957:Plasmids / Q000502:physiology*