Production of nerve growth factor in rat skeletal muscle.
Production of the nerve growth factor (NGF) was confirmed by Northern blot hybridization using a specific probe of synthetic cDNA. In normal rat skeletal muscle, this probe clearly showed a band equivalent to 1.3 kilobases (kb) of messenger RNA (mRNA) of NGF of male mouse submaxillary gland. By denervation, the density of the bands derived from muscles increased by a factor of more than 3 at 4 and 6 days later compared to the control. The synthesis of mRNA of NGF in muscle was also confirmed following tetrodotoxin (TTX) blockade of sciatic nerve without denervation.
Amano
T
T
Department of Neuroscience, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Yamakuni
T
T
Okabe
N
N
Sakimura
K
K
Takahashi
Y
Y
D000818:Animals; D015152:Blotting, Northern; D005260:Female; D009121:Muscle Denervation; D009132:Muscles / Q000187:drug effects / Q000294:innervation / Q000502:physiology*; D009414:Nerve Growth Factors / Q000235:genetics*; D011061:Poly A / Q000032:analysis / Q000235:genetics; D012313:RNA / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013779:Tetrodotoxin / Q000494:pharmacology
D009414:Nerve Growth Factors; D012333:RNA, Messenger; D011061:Poly A; D013779:Tetrodotoxin; D012313:RNA