Epitope mapping of snake venom phospholipases A2 with pseudexin monoclonal antibodies.
Fifteen different monoclonal antibodies, developed against a pseudexin A, B, and C mixture, were screened for linear epitope recognition. Peptides (9-mers) spanning pseudexin B were synthesized on alanine-derivatized polyethylene pins and subsequently probed with antibody. Four antibodies recognized linear epitopes of pseudexin A, pseudexin B, and also nonidentical sequences found in other phospholipases A2 (PLA2S) as determined by enzyme-linked immunosorbent assays. Three antibodies recognized a highly conserved site important in calcium binding and the interlocking of dimeric forms of PLA2. Antibodies neutralizing lethal or enzymatic effects of PLA2 did not recognize linear epitopes.
Stiles
B G
BG
Department of Toxinology, U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21702-5011.
Middlebrook
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism; D003198:Computer Simulation; D004546:Elapid Venoms / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010741:Phospholipases A / Q000737:chemistry / Q000276:immunology*; D054467:Phospholipases A2; D012910:Snake Venoms / Q000737:chemistry*; D013552:Swine
D000911:Antibodies, Monoclonal; D004546:Elapid Venoms; D000939:Epitopes; C026235:Pseudechis venom; D012910:Snake Venoms; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium