[Histamine release test of patients allergic to the house dust mite, Dermatophagoides farinae--analysis according to various clinical backgrounds].
Since the histamine release test is more complicated than some of the other tests, there are smaller number of clinical studies employing this method. In this study, we selected 25 males and 20 females, allergic to the house dust mite, to undergo the histamine release test challenged by 7 serial concentrations of the mite allergen diluted from 10 times to 10 million times the original allergen at a concentration of 0.01%. Histamine release was expressed as a percentage of the total histamine concentration contained in the cells. Allergen induced dose response curve of histamine release was drawn for each patient, and in order to characterize each curve, 5 different indices were selected and were averaged according to the clinical backgrounds of the patients. Significant increases in histamine release were observed in males under 25 and females above 26 years of age. Histamine release rate in patients with RAST score of above 3+ was significantly more enhanced than in those with RAST score of below 2+, though this result was significant only for those under 25 years of age. Blood samples taken in June-August, and September-November, showed significant increases in the histamine release, though the indices for enhancement differed in both seasons. When analyzed according to the numbers of years the patient was desensitized, the results showed, contrary to our expectations, a more increased release in the desensitized group than in the non-desensitized group.
Haida
M
M
Department of Medicine and Physical Therapy, University of Tokyo.
Suko
M
M
Okudaira
H
H
Ito
K
K
Miyamoto
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D004391:Dust; D005260:Female; D006636:Histamine Release / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000276:immunology*
D004391:Dust