Protection of mice from a lethal coronavirus infection in the central nervous system by adoptive transfer of virus-specific T cell clones.
The protective effect of a mouse hepatitis virus type-4 (MHV-4)-specific CD8+ cytotoxic T cell clone and a CD4+ helper T cell clone was examined by the adoptive transfer into brains of mice lethally infected with MHV-4. Mice survived acute encephalitis if more than 5 x 10(5) cells of either type of the virus-specific T cell clones had been transferred into H-2-matched recipients by 1 day post-infection. Although the adoptive transfer of both types of the T cell clones suppressed viral growth and viral antigen-positive cells in the brains, a significant inhibition of virus replication by the cytotoxic T cell clone was detected prior to that induced by the helper T cell clone. Histologically, cell destruction was prominent in the brains of mice which received the cytotoxic T cell clone. These results demonstrate that both the CD8+ cytotoxic T cell and the CD4+ helper T cell can protect mice from a lethal MHV-4 infection in the central nervous system.
Yamaguchi
K
K
Department of Animal Pathology, University of Tokyo, Japan.
Goto
N
N
Kyuwa
S
S
Hayami
M
M
Toyoda
Y
Y
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D001927:Brain Diseases / Q000276:immunology* / Q000382:microbiology; D002999:Clone Cells; D000939:Epitopes; D006524:Hepatitis, Viral, Animal / Q000276:immunology* / Q000382:microbiology; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D006517:Murine hepatitis virus / Q000276:immunology* / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology / Q000637:transplantation*
D000956:Antigens, Viral; D000939:Epitopes