title: Ultrastructural characteristics of rat peritoneal mast cells undergoing differential release of serotonin without histamine and without degranulation.
abstract: Rat mast cells pretreated with the tricyclic antidepressant drug amitriptyline and stimulated with compound 48/80 secreted 60% of the total serotonin present in the cells, but only 15% of histamine, another amine stored in the same granules. Ultrastructural studies demonstrated that mast cells undergoing such differential release do not exhibit classical degranulation by compound sequential exocytosis. However, there were changes in granule shape and size, as well as alterations in many morphometric parameters consistent with secretion. Storage granules lost their homogeneity, exhibited greatly reorganized matrix and were surrounded by clear spaces which were often associated with small (0.1-0.01 microns) cytoplasmic vesicles, some of which contained electron-dense material. Secretory granules often had bud-like protrusions or were fused together in series. Quantitative autoradiography localized 3H-serotonin outside the storage granules, close to small vesicles, while staining with ruthenium red demonstrated that vesicular structures associated with differential release were not endocytotic. These results suggest that amitriptyline may inhibit regular exocytosis and permit at least serotonin to be moved selectively from storage granules to the cytosol or small vesicles from which it is eventually released.
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut.
authors.forename: S
authors.initials: S
authors.lastname: Kraeuter Kops
authors.forename: T C
authors.initials: TC
authors.lastname: Theoharides
authors.forename: C T
authors.initials: CT
authors.lastname: Cronin
authors.forename: M G
authors.initials: MG
authors.lastname: Kashgarian
authors.forename: P W
authors.initials: PW
authors.lastname: Askenase
mesh_terms: D000639:Amitriptyline / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D015550:Cell Degranulation / Q000187:drug effects / Q000502:physiology*; D002462:Cell Membrane / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology; D006636:Histamine Release / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008407:Mast Cells / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010529:Peritoneal Cavity / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D012430:Ruthenium Red; D012701:Serotonin / Q000378:metabolism*; D014316:Tritium; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D014316:Tritium; D012430:Ruthenium Red; D000639:Amitriptyline; D012701:Serotonin; D003189:p-Methoxy-N-methylphenethylamine