title: Reproductive death of Chinese hamster V79 cells after exposure to chemical inhibitors of DNA synthesis.
abstract: 1. The results of this study have contributed to the definition of three categories of chemical inhibitors of DNA replication in mammalian cells. 2. Inhibitors of replicon cluster initiation [4-nitroquinoline-N-oxide (4-NQO), etoposide (VP-16), teniposide (VM-26), amsacrine (m-AMSA), N-methyl-N'-nitro-N-nitrozoguanidine (MNNG), cis-Pt(II)diammine dichloride (cis-PDD)], which needed similar doses to produce a slow and persistent (up to 4 hr) inhibition of DNA synthesis, followed by significant cell killing. 3. Inhibitors of DNA replication by indirect action [3-aminobenzamide [correction of 3-aminobezamide] (3-AB), cycloheximide (CHX), puromycin (PRC), bisbenzimide Hoechst No. 33258 (H-33258]), that showed reduced cytotoxic effects, and caused a slow (60 min) and reversible inhibition of DNA synthesis. 4. Inhibitors of formation and/or polymerization of deoxyribonucleotides [5-aminouracil (5-AU), bisbenzimide Hoechst No. 33342 (H-33342)], which induced a fast (20 min) and reversible suppression of DNA replication, associated with limited cell killing.
authors.affiliation: Department of Radiology and Radiation Biology, Colorado State University, Fort Collins 80523.
authors.forename: D
authors.initials: D
authors.lastname: Suciu
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000677:Amsacrine / Q000494:pharmacology; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D006690:Bisbenzimidazole / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D003513:Cycloheximide / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects*; D005047:Etoposide / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D066298:In Vitro Techniques; D007700:Kinetics; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D013713:Teniposide / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*; D014498:Uracil / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D001549:Benzamides; D000677:Amsacrine; D008769:Methylnitronitrosoguanidine; D011691:Puromycin; D015112:4-Nitroquinoline-1-oxide; D014498:Uracil; D005047:Etoposide; C025160:3-aminobenzamide; D013713:Teniposide; D003513:Cycloheximide; D006690:Bisbenzimidazole; D002945:Cisplatin; C011166:5-aminouracil