title: Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood.
abstract: Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.
authors.affiliation: Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilian University, Munich, Germany.
authors.forename: T F
authors.initials: TF
authors.lastname: Schwarz
authors.forename: S
authors.initials: S
authors.lastname: Serke
authors.forename: B
authors.initials: B
authors.lastname: Hottenträger
authors.forename: A
authors.initials: A
authors.lastname: von Brunn
authors.forename: H
authors.initials: H
authors.lastname: Baurmann
authors.forename: A
authors.initials: A
authors.lastname: Kirsch
authors.forename: W
authors.initials: W
authors.lastname: Stolz
authors.forename: D
authors.initials: D
authors.lastname: Huhn
authors.forename: F
authors.initials: F
authors.lastname: Deinhardt
authors.forename: M
authors.initials: M
authors.lastname: Roggendorf
mesh_terms: D001769:Blood; D015139:Blotting, Southern; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins