title: Age-related development of olfactory bulb transplants in rats.
abstract: We are using the rat olfactory system to study developmental aspects of neurotransplantation (TX). Age-related TX maturation and subsequent establishment of connections are of special concern. Previous studies of deafferentation by olfactory bulb (OB) removal suggested "critical" periods of plasticity in the system. We present here preliminary attempts at relating age of host receiving TX to maturation of the TX and its connections. This investigation used hosts of postnatal age (PN) 13-14 days with fetal donors at Embryonic Day 15; the former having one OB ablated and receiving a fetal donor OB TX immediately placed in the vacated space. The fetal tissue was labeled previously in utero with tritiated thymidine. After 2 months a small coagulation lesion was placed in the OB TX and 2 days later the tissue was taken, serially sectioned, and processed for [3H] autoradiography, degeneration, and olfactory marker protein (OMP). Extensively 3H-labeled OB TXs with localized small lesions were studied. The cellular architecture of the TX is less well organized than in normals but substantial OMP reactivity occurs throughout. Degeneration occurs mainly near the lesion and little if any degeneration is seen beyond the 3H-labeled TX tissue. The results show that OB TX survive and develop in the PN 13-14 age group as they do in the younger animals and that primary olfactory neurons likewise reinnervate the TX but that PN 13-14 TX efferent projections are far more limited than those of younger hosts.
authors.affiliation: Department of Neurological Surgery, University of Washington, Seattle 98195.
authors.forename: L E
authors.initials: LE
authors.lastname: Westrum
authors.forename: J N
authors.initials: JN
authors.lastname: Kott
authors.forename: H
authors.initials: H
authors.lastname: Vickland
authors.forename: X M
authors.initials: XM
authors.lastname: Dong
mesh_terms: D000375:Aging; D000818:Animals; D001345:Autoradiography; D016380:Brain Tissue Transplantation / Q000502:physiology*; D016332:Fetal Tissue Transplantation / Q000502:physiology; D009830:Olfactory Bulb / Q000166:cytology / Q000254:growth & development* / Q000637:transplantation*; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D014316:Tritium; D013936:Thymidine