Thiopental and ether anaesthesia decrease the prostaglandin receptor density of the rat liver.
Male Sprague-Dawley rats were killed either by decapitation or during anaesthesia with thiopental or diethylether by aortectomy. Livers were removed and liver plasma membranes were prepared using standard techniques. Direct binding experiments with 3H-PGE1 and 3H-iloprost revealed heterogeneity of the binding sites (high and low affinity binding sites), whereas 3H-PGE2 demonstrated only high affinity binding to the liver. The highest binding capacity for all radioligands was found for livers after decapitation. Livers obtained during anaesthesia showed a significantly (p less than 0.05 to p less than 0.001) lower binding capacity and binding affinity for 3H-PGE1, 3H-PGE2 and 3H-iloprost. The reduction in binding activity was more pronounced in livers obtained during inhalation than thiopental anaesthesia. Specific binding amounted to 82.1 +/- 7% for 3H-PGE2, 75.3 +/- 9% for 3H-PGE1 and 78.9 +/- 8% for iloprost in livers obtained after decapitation. In livers obtained during anaesthesia specific prostaglandin binding was significantly (p less than 0.01) decreased, again being more pronounced during inhalation than thiopental anaesthesia. These results suggest that some anaesthetics interfere with prostaglandin receptors of the liver.
Virgolini
I
I
Atherosclerosis Research Group (ASF) Vienna, University of Vienna, Austria.
Peskar
B A
BA
Sinzinger
H
H
D000527:Alprostadil / Q000378:metabolism; D000758:Anesthesia; D000818:Animals; D015232:Dinoprostone / Q000378:metabolism; D015536:Down-Regulation; D004986:Ether / Q000494:pharmacology*; D016285:Iloprost / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011982:Receptors, Prostaglandin / Q000187:drug effects* / Q000378:metabolism; D012107:Research Design; D013874:Thiopental / Q000494:pharmacology*
D011982:Receptors, Prostaglandin; D004986:Ether; D000527:Alprostadil; D016285:Iloprost; D013874:Thiopental; D015232:Dinoprostone