Cell-free system responsible for internal radiolabeling of glycopeptidolipids of the Mycobacterium avium complex.
Internal radiolabeling of serotype-specific glycopeptidolipids with [14C]mannose was accomplished with a cell-free system derived from serotype 20 of the Mycobacterium avium complex. Similar radiolabeling was not apparent with a cell-free system derived from the rough colony variant, previously shown to be devoid of glycopeptidolipids. Although a comparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein analysis of the parent and rough variant strains revealed a close similarity, there were some proteins unique to the parent strain.
Ramasesh
N
N
Department of Microbiology and Immunology, Texas College of Osteopathic Medicine, Fort Worth 76107.
Wright
E L
EL
Barrow
W W
WW
D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D006001:Glycoconjugates; D006020:Glycopeptides; D008358:Mannose / Q000378:metabolism; D015269:Mycobacterium avium Complex / Q000378:metabolism*; D011510:Proteolipids
D002250:Carbon Radioisotopes; D006001:Glycoconjugates; D006020:Glycopeptides; D011510:Proteolipids; D008358:Mannose