title: [Synthesis of peptides of the preS1-region of the viral hepatitis B envelope protein and localization of antigenic determinants].
abstract: We synthesized the 24-41, 30-36, 31-36, 24-30 fragments of the preS1-region of the hepatitis B (subtype ayw) envelope. The peptides were prepared by the solid phase synthesis on perfluorpolyethylene polymer grafted with polystyrene. The peptide chains were elongated from C-terminus using activated esters and symmetrical anhydrides of Boc-amino acids, cleaved off the solid phase by HBr or TFMSA in TFA, purified by gel filtration, and, after conjugation with protein carriers, inoculated into test animals. The resultant antibodies were shown to react with peptides. The blood sera from patients with acute hepatitis B reacted with the conjugates of peptides 24-41, 30-36, 31-36 in the immunoenzymic solid phase assay. The monoclonal antibodies for the preS1-polypeptide were shown to react with peptides 24-41, 30-36, 31-36 and with their conjugates. The results obtained were proved by the data of the epitope-mapping with overlapping hexapeptides.
authors.forename: E I
authors.initials: EI
authors.lastname: Prokuronova
authors.forename: I N
authors.initials: IN
authors.lastname: Khlebnikova
authors.forename: G A
authors.initials: GA
authors.lastname: Zheltukhina
authors.forename: R P
authors.initials: RP
authors.lastname: Evstigneeva
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Semiletov
authors.forename: V A
authors.initials: VA
authors.lastname: Karpova
authors.forename: T I
authors.initials: TI
authors.lastname: Kalinina
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Khudiakov
authors.forename: R P
authors.initials: RP
authors.lastname: Pavliuchenkova
authors.forename: V D
authors.initials: VD
authors.lastname: Smirnov
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D000939:Epitopes / Q000378:metabolism*; D006509:Hepatitis B / Q000276:immunology / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D014759:Viral Envelope Proteins / Q000276:immunology / Q000378:metabolism*
chemical_list: D000939:Epitopes; D010455:Peptides; D014759:Viral Envelope Proteins
vernacular_title: Sintez peptidov preS1-oblasti belka obolochki virusa gepatita B i lokalizatsiia antigennoĭ determinanty.