Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein.
By differential screening of a Xenopus egg cDNA library, we selected nine clones (Eg1 to Eg9) corresponding to mRNAs which are deadenylated and released from polysomes soon after fertilization. The sequence of one of these clones (Eg5) revealed that the corresponding protein has the characteristic features of a kinesin-related protein. More specifically, Eg5 was found to be nearly 30% identical to a kinesin-related protein encoded by bimc, a gene involved in nuclear division in Aspergillus nidulans.
Le Guellec
R
R
Laboratoire de Biologie et Génétique du Développement, Centre Nationale de la Recherche Scientifique, URA 256, Université de Rennes, France.
Paris
J
J
Couturier
A
A
Roghi
C
C
Philippe
M
M
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001230:Aspergillus / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004330:Drosophila / Q000235:genetics; D004625:Embryo, Nonmammalian; D006801:Humans; D016547:Kinesins; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012568:Schizosaccharomyces / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014981:Xenopus
D012333:RNA, Messenger; D012313:RNA; D000251:Adenosine Triphosphatases; D016547:Kinesins