title: Immune complexes as immunizing agents to increase the number of monoclonal antibody producing hybrids and to deviate the response to poorly immunogenic epitopes.
abstract: The use of immune complexes (IC) in an antibody excess, as immunizing agent, led to a large increase in the mouse polyclonal response to human SIgA. This enhanced response, as compared to SIgA alone, was analysed with mouse polyclonal anti-alpha chain antibodies (Ab). A kinetic study showed an early rise (between days 14 and 21) of the antibody response against the discontinuous epitopes of SIgA while the anti-IgA response increase was delayed. Induction of hybridomas with an IC consisting in SIgA containing an excess of anti-alpha chain Ab, increased 10-fold the number of positive wells. Moreover, two of these MAb were specific for weakly immunogenic epitopes. One recognized only SIgA (anti-C), i.e. the association between the alpha chain and the secretory component (SC), while the other mainly combined to IgA dimers (anti-P). Both these MAb will be useful tools for structural studies and for the dosage of secretory Ab.
authors.affiliation: Institut Pasteur, Immunologie Microbienne, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Bouige
authors.forename: S
authors.initials: S
authors.lastname: Iscaki
authors.forename: J
authors.initials: J
authors.lastname: Pillot
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D007700:Kinetics; D051379:Mice
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007071:Immunoglobulin A, Secretory