Monoclonal antibody with specificity to a conserved epitope in the C-terminal domain of histone H1 variants.
A monoclonal type M-immunoglobulin (IgM) was generated in mice against a nuclease-urea extract of HeLa metaphase chromosomes. This antibody stains metaphase chromosomes from a variety of mammalian cultured cell types by indirect immunofluorescence. Antibody 12C7 reacts by western transfer technique with histone H1 in all the cell lines tested. The antibody cross-reacts with H1, and H1(0) in human cells. Proteolytic digestions of H1 suggest that the epitope is localized in the carboxy-terminal domain of the histone H1 molecule. Digestion with trypsin demonstrates that the antibody 12C7 does not react with the globular domain of histone H1. The C-terminal domain of H1 subtypes therefore seems to have a conserved determinant which does exist in H1, H1(0), and probably in H5. This antibody has applications in studying the role of that domain of H1 in processes like chromosome condensation and variations in chromatin structure which influence gene expression.
Ortiz
M
M
Facultad de Ciencias, Universidad de Cádiz, Spain.
Bejarano
L A
LA
Rendón
M C
MC
Martin
G
G
Brinkley
B R
BR
Valdivia
M M
MM
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D002455:Cell Division / Q000276:immunology; D002843:Chromatin / Q000276:immunology; D002877:Chromosomes, Human / Q000648:ultrastructure; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000276:immunology; D006657:Histones / Q000276:immunology*; D006801:Humans; D010449:Peptide Mapping; D011110:Polymorphism, Genetic / Q000276:immunology; D011487:Protein Conformation
D000911:Antibodies, Monoclonal; D002843:Chromatin; D000939:Epitopes; D006657:Histones