The induction and repair of (6-4) photoproducts in Neurospora crassa.
The (6-4) photoproduct lesion found in DNA after UV irradiation is repaired by germinating Neurospora crassa conidia. Wild-type Neurospora removes 80% of the (6-4) photoproduct in approximately 20 min and maximal repair is accomplished by 30 min with approximately 89% of the original lesions removed. Mutagen-sensitive Neurospora mutants belonging to the established excision repair epistasis group, UVS-2, are not defective in the removal of cyclobutane pyrimidine dimers. Furthermore, we find these mutants capable of removing (6-4) photoproducts from their DNA at a rate similar to wild type. Comparable kinetics are also observed in key members of the other two epistasis groups.
Baker
T I
TI
Department of Microbiology, School of Medicine, University of New Mexico, Albuquerque 87131.
Radloff
R J
RJ
Cords
C E
CE
Engel
S R
SR
Mitchell
D L
DL
D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D009153:Mutagens / Q000494:pharmacology; D009492:Neurospora crassa / Q000235:genetics* / Q000528:radiation effects; D011740:Pyrimidine Dimers / Q000032:analysis; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D009153:Mutagens; D011740:Pyrimidine Dimers