Centrifugal growth in orthotopic grafts of allogeneic dorsal root ganglia in adult rats: evidence for possible central ingrowth?
Fetal allogeneic dorsal root ganglion (DRG) transplants from 13-15 day rat embryo's (E13-E15) survived and differentiated when grafted orthopically (within the capsules of the excised 4th and 5th lumbar (L4-L5) ganglia) in adult rats. Survival of grafted neurones was established by prelabeling the grafts with a fluorescent vital dye (DiI) and visualizing the retained fluorescent marker 3 to 9 months later. Simultaneous retrograde tracing using fluorescent tracers applied in the spinal cord and peripheral nerve, respectively, yielded double-labeled dorsal root ganglion neurons, some of which were prelabeled. These findings demonstrate that prelabeled E13-E15 ganglia survive orthopic grafting, organotypically differentiate into mature DRG neurones, and can be double-labeled with fluorescent dyes applied to their peripherally and centrally directed processes. The presence of DiI containing cells which were retrogradely labeled from the spinal cord suggests that fetal (E13-E15) ganglia may have the capability of growing into a mature spinal cord.
Rosario
C M
CM
Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
Aldskogius
H
H
Carlstedt
T
T
Sidman
R L
RL
D000375:Aging; D000818:Animals; D002232:Carbocyanines; D005260:Female; D016332:Fetal Tissue Transplantation / Q000502:physiology; D005456:Fluorescent Dyes; D005727:Ganglia, Spinal / Q000166:cytology / Q000254:growth & development*; D005865:Gestational Age; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology / Q000254:growth & development*; D014184:Transplantation, Homologous
D002232:Carbocyanines; D005456:Fluorescent Dyes; C024286:3,3'-dioctadecylindocarbocyanine