Determination of 5-methoxyindoles in pineal gland and plasma samples by high-performance liquid chromatography with electrochemical detection.
A liquid chromatographic analysis with electrochemical detection of 5-methoxytryptamine, 5-methoxytryptophol, 5-methoxyindoleacetic acid and melatonin is described. Optimal elution conditions were determined by studying several variables: pH, buffer salt, counter ion and organic modifier. Measurement of 5-methoxyindoles in the pineal gland and plasma of hamsters has been performed after extraction. This method is specific and sensitive, and enables detection of 5-methoxyindoles in a pool of two hamster pineal glands. This is also the first time that these three 5-methoxyindoles have been measured simultaneously in plasma.
Raynaud
F
F
URA CNRS 1332, Université Louis Pasteur, Strasbourg, France.
Pévet
P
P
D008735:5-Methoxytryptamine / Q000032:analysis* / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006224:Cricetinae; D006863:Hydrogen-Ion Concentration; D006897:Hydroxyindoleacetic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D007211:Indoles / Q000032:analysis* / Q000097:blood; D008027:Light; D008297:Male; D008550:Melatonin / Q000032:analysis / Q000097:blood; D008647:Mesocricetus; D010870:Pineal Gland / Q000737:chemistry*
D000975:Antioxidants; D002021:Buffers; D007211:Indoles; C001736:5-methoxyindoleacetic acid; D008735:5-Methoxytryptamine; D006897:Hydroxyindoleacetic Acid; C002465:methoxytryptophol; D008550:Melatonin