Cytosolic and membrane-bound nitric oxide synthase.
Cytotoxic activated macrophages were sonicated and centrifuged. The activity of nitric oxide (NO) synthase was present in the supernatant and independent of Ca2+. The pellets were washed three times and treated with buffer containing 0.1% Triton X-100 or buffer alone, followed by centrifugation. The supernatant containing Triton X-100 showed NO synthase activity that was dependent on Ca2+, whereas the supernatant without the detergent had little activity. These data suggest that there are two forms of NO synthase: cytosolic and membrane-bound enzymes.
Hiki
K
K
Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Yui
Y
Y
Hattori
R
R
Eizawa
H
H
Kosuga
K
K
Kawai
C
C
D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D008264:Macrophages / Q000737:chemistry; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D019001:Nitric Oxide Synthase; D051381:Rats; D011919:Rats, Inbred Strains
D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D008274:Magnesium; D002118:Calcium