A simple fluorescent technique for the location of tumour cells in frozen sections of the head and neck region.
Tumour cells possess cell surface proteases referred to as guanidinobenzoatase (GB) which are closely similar to plasminogen activator. Previous studies have demonstrated different isoenzymic forms of GB on tumour cells and normal cells which can be recognised by cytoplasmic protein inhibitors extracted from frozen sections of appropriate tissues. We now show that normal human serum possesses inhibitors which selectively recognise the isoenzymic forms of GB associated with normal cell surfaces but do not recognise the GB on tumour cell surfaces in frozen sections of tissue obtained from the head and neck regions.
Steven
F S
FS
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, UK.
Griffin
M M
MM
Maier
H
H
Born
A
A
Zoeller
J
J
D000585:Aminacrine; D014408:Biomarkers, Tumor / Q000032:analysis; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D010450:Endopeptidases; D005453:Fluorescence; D005629:Frozen Sections; D006258:Head and Neck Neoplasms / Q000175:diagnosis / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D013194:Staining and Labeling
D014408:Biomarkers, Tumor; D007527:Isoenzymes; D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases