A chloride channel in rat and human axons.
Current recordings from single chloride channels were obtained from excised and cell-attached patches of rat and human axons. In rat axons the channels showed an outwardly rectifying current-voltage relationship with a slope conductance of 33 pS at negative membrane potentials and 65 pS at positive potentials (symmetrical 150 mM CsCl). They were measurably permeable for cations (PNa/PCs/PCl = 0.1/0.2/1). Channel currents were independent of cytoplasmatic calcium concentration. Inactivation was not observed and gating was weakly voltage dependent. Cl- channels in human axons showed similar gating behavior but had a lower conductance.
Strupp
M
M
Department of Physiology, University of Munich, F.R.G.
Grafe
P
P
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002586:Cesium / Q000494:pharmacology; D018118:Chloride Channels; D002712:Chlorides; D004553:Electric Conductivity; D006801:Humans; D007473:Ion Channels / Q000502:physiology*; D008297:Male; D008536:Meglumine / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology; D013126:Spinal Nerve Roots / Q000502:physiology*; D013497:Sural Nerve / Q000502:physiology*
D018118:Chloride Channels; D002712:Chlorides; D007473:Ion Channels; D008565:Membrane Proteins; D002586:Cesium; D012965:Sodium Chloride; D008536:Meglumine; C028019:cesium chloride