title: New rapid staining methods of Cryptosporidium oocysts in stools.
abstract: Two new extemporaneous negative-staining methods are proposed to detect Cryptosporidium oocysts in stools, using light-green and merbromine in 1% and 2% aqueous solution, respectively. A Ziehl-Neelsen stain as modified by Henriksen and Polhenz was used as a reference technique. A comparison between these two new stains and the reference, a modified Ziehl-Neelsen mod. method gave almost identical sensitivity and specificity. We propose their use in routine diagnosis for enteric cryptosporidiosis.
authors.affiliation: Department of Infectious Diseases, University, IRCCS San Matteo, Pavia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Chichino
authors.forename: A
authors.initials: A
authors.lastname: Bruno
authors.forename: C
authors.initials: C
authors.lastname: Cevini
authors.forename: C
authors.initials: C
authors.lastname: Atzori
authors.forename: S
authors.initials: S
authors.lastname: Gatti
authors.forename: M
authors.initials: M
authors.lastname: Scaglia
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000469:parasitology; D000818:Animals; D003458:Cryptosporidium / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology*; D006679:HIV Seropositivity / Q000469:parasitology; D006801:Humans; D008297:Male; D008622:Merbromin; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D008622:Merbromin