Schistosoma mansoni: ingestion of dextrans, serum albumin, and IgG by schistosomula.
Schistosomula of Schistosoma mansoni develop the ability to ingest and digest red blood cells after the fourth day post-transformation. Here, we have used fluorescently-labeled dextrans and two plasma proteins, albumin and IgG, to test whether day-old schistosomula can ingest and process macromolecules prior to the time that they eat red cells. Worms ingested dextrans of molecular weights 4,000, 70,000 and 2 x 10(6) in a time- and concentration-dependent manner. The dextran remained in the cecal lumen for up to 2 days after feeding. Parasites ingested both fluorescein-conjugated bovine serum albumin and rabbit IgG, but neither of these proteins remained confined to the cecum over time. Instead, fluorescence redistributed to the acetabular glands within a few hours. Thin-layer chromatography indicated that schistosomula degraded fluorescein-conjugated albumin to fluorescein-conjugated peptides approximately 10-15 amino acids long. The volume of the cecum was estimated to be 2431 microns 3 and the surface area 299 microns 2. These results demonstrate that larval schistosomes can ingest both proteins and complex carbohydrates shortly after transformation, before they can ingest red cells. Further, the gut apparently releases proteases that cleave plasma proteins, but not saccharidases that cleave dextran.
Bennett
M W
MW
Program in Cell and Development Biology, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Caulfield
J P
JP
D000818:Animals; D002432:Cecum / Q000033:anatomy & histology; D003911:Dextrans / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D005452:Fluoresceins / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism*; D012235:Rhodamines; D012550:Schistosoma mansoni / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013861:Thiocyanates
D003911:Dextrans; D005452:Fluoresceins; D007074:Immunoglobulin G; D012235:Rhodamines; D013861:Thiocyanates; C039217:fluorescein isothiocyanate bovine serum albumin; C015219:fluorescein isothiocyanate dextran; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate