[Monoclonal antibody MIA15-5, which inhibits cell motility and metastatic potential, has a great effect on the prognosis of the post-surgical patients with lung cancer].
The functional monoclonal antibody, selected based on the inhibition of cell motility, was found to be directed to specific carbohydrate structure Fuc alpha 1----2 Gal beta 1----R. This monoclonal antibody, MIA15-5 was established after immunization of mice with adenocarcinoma line of the lung PC7, and selected based on inhibition of U937, HEL, and MAC10 cell migration due to the transwell assay. MIA15-5 reacted with 30-40% of high metastatic variant BL6 of mouse melanoma B16 line and metastatic deposition to lung after injection of BL6 cells was strongly inhibited if MIA15-5 was injected within 3 hours, but was not inhibited by injection of other anti-H antibodies under the same conditions. Injection of MIA15-5 had much less inhibitory effect if administered 1 day after BL-6 injection, and no effect at 3 days. In immunohistochemical staining of 149 formalin fixed specimens of patients with lung cancer, especially adenocarcinoma has the highly frequency of MIA15-5 (65.4%, 49 of 75 cases) and the five year survival rate of the patients with negative staining of MIA15-5 was 58.6% and much better than one with positive staining, 20.9%.
Miyake
M
M
Kitano Hospital, Osaka, Japan.
Taki
T
T
Hakomori
S
S
D000230:Adenocarcinoma / Q000276:immunology / Q000401:mortality; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D000939:Epitopes; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology / Q000401:mortality*; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000276:immunology
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D000939:Epitopes