Decreased expression of the myristoylated alanine-rich C kinase substrate in transformed BALB/C 3T3 mouse fibroblasts.
Expression of the myristoylated alanine-rich C kinase substrate was studied in the untransformed A31 and the transformed BPA31, DA31 and KA31 BALB/c 3T3 mouse fibroblast cell lines. The MARCKS transcript was markedly reduced in the three transformed cell lines. Southern analysis revealed similar gene copy number and no apparent DNA rearrangement. In the untransformed A31 cells, the MARCKS mRNA was constitutive in the cell cycle.
Otsuka
M
M
Division of Medical Oncology, Harbor-UCLA Medical Center, Torrance 90509.
Yang
H C
HC
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D000818:Animals; D001564:Benzo(a)pyrene / Q000494:pharmacology; D015139:Blotting, Southern; D002453:Cell Cycle; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D015342:DNA Probes; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D015870:Gene Expression; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007708:Kirsten murine sarcoma virus / Q000235:genetics*; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D011493:Protein Kinase C / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics
D015342:DNA Probes; D047908:Intracellular Signaling Peptides and Proteins; C000619710:MARCKS protein, human; C000619711:Marcks protein, mouse; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D001564:Benzo(a)pyrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D012313:RNA; D011493:Protein Kinase C