title: Retinoic acid regulates, in vitro, the two normal pathways of differentiation of human laryngeal keratinocytes.
abstract: We have investigated the regulation of the two normal differentiation pathways followed by laryngeal epithelium. Using a tissue culture system that permits growth of cells at the air-liquid interface in serum-free medium, we found that modulating the concentration of retinoic acid is sufficient to determine which pathway is used. At 10(-8) M retinoic acid, the cells form a stratified squamous epithelium which expresses the differentiation-specific keratin K13. At 10(-7) M retinoic acid, the cells form a ciliated pseudostratified epithelium, with no expression of K13. These results are distinct from those seen with foreskin keratinocytes, which have only a single pathway of normal differentiation.
authors.affiliation: Department of Otolaryngology, Long Island Jewish Medical Center, New Hyde Park, New York 11042.
authors.forename: M G
authors.initials: MG
authors.lastname: Mendelsohn
authors.forename: T P
authors.initials: TP
authors.lastname: Dilorenzo
authors.forename: A L
authors.initials: AL
authors.lastname: Abramson
authors.forename: B M
authors.initials: BM
authors.lastname: Steinberg
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002923:Cilia / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D007633:Keratins / Q000378:metabolism; D007830:Larynx / Q000166:cytology*; D008854:Microscopy, Electron; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D014212:Tretinoin; D007633:Keratins