Antisera raised against recombinant soluble CD4s can have reactivity to recombinant HIV-1 envelope glycoproteins.
Antisera from sheep immunized with two different recombinant soluble CD4 preparations both exhibited cross-reactive antibodies specific for recombinant gp120s and gp160s prepared in different expression systems in immunoblots and radiobinding assays. The cross-reactivity was broadly mapped to the amino-terminal 70K cleavage fragment of gp120 and more closely identified to residues 204-274 using a series of truncated recombinant gp120s. However, this reactivity could not be detected against virally encoded gp160/120.
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Division of Immunobiology, National Institute for Biological Standards and Control, Potters Bar, Herts, UK.
Patel
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Dilger
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Ling
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Thorpe
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D000818:Animals; D015704:CD4 Antigens / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D015686:Gene Products, env / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D019302:HIV Envelope Protein gp160; D007106:Immune Sera / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D011498:Protein Precursors / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D012756:Sheep / Q000276:immunology
D015704:CD4 Antigens; D000939:Epitopes; D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D007106:Immune Sera; D010446:Peptide Fragments; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins