Tryptic mapping by reversed phase liquid chromatography.
This chapter reviews the technique of tryptic mapping by reversed phase liquid chromatography by describing its fundamental principles, present status, and recent advances as well as its uses and limitations. Two commonly used S-carboxymethylation/tryptic digestion procedures are documented. Column and instrumental requirements are described and illustrated with examples. Mobile phase selection and operating variables (tG, F) are also discussed in terms of their effects on both peptide resolution and detection sensitivity.
Dong
M W
MW
Perkin-Elmer Corporation, Norwalk, Connecticut.
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D010959:Tissue Plasminogen Activator / Q000032:analysis; D014357:Trypsin
D014357:Trypsin; D010959:Tissue Plasminogen Activator