[Morphological demonstration of function-dependent cellular calcium redistributions in secretory cells by x-ray microanalysis, LAMMA and fluorescence cytometry].
Cytosolic calcium and its parathyroid hormone induced increase was evaluated in rat osteosarcoma cells by quin 2 fluorometry. Electron microscopic calcium detection in depot organelles (i.e. the recently defined calciosomes) is improved by a new precipitation method with hydroxylamine naphthoic acid and fluoride combined with X-ray microanalysis. As shown in submandibulary gland and pancreatic B cells, a new combination of GBHA staining with laser microprobe mass analysis (LAMMA) for the first time enables direct kinetical studies of calcium at the microscopical level using stable calcium isotopes (i.e. Ca44) as tracers.
Schäfer
H
H
Institut für Pathologie, Universität Hamburg.
Seydel
U
U
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D004577:Electron Probe Microanalysis / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D007834:Lasers; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D051381:Rats; D012513:Sarcoma, Experimental / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure
D002118:Calcium
Morphologische Darstellung funktionsabhängiger zellulärer Kalziumverschiebungen in sekretorischen Zellen mit Röntgenmikroanalyse, LAMMA und Fluoreszenz-Zytometrie.