Human immunodeficiency virus type 1 infection of endothelial cells in vitro.
In order to establish whether endothelial cells are involved in immunodeficiency virus type 1 (HIV-1) infection, we performed a virological study on endothelial cells isolated from human adipose tissue and infected with HIV-1 in vitro. Supernatants from cultures showed a reverse transcriptase activity starting one day after HIV inoculation. Viral rescue was significantly impaired in cycloheximide treated cells confirming a de novo synthesis of viral products.
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Institute of Microbiology, University of Bologna, S. Orsola Hospital, Italy.
Furlini
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Cenacchi
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Preda
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La Placa
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D000273:Adipose Tissue; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004727:Endothelium / Q000166:cytology / Q000382:microbiology*; D015683:Gene Products, gag / Q000032:analysis; D015488:HIV Antigens / Q000032:analysis; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000201:enzymology / Q000276:immunology / Q000502:physiology*; D006801:Humans; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D014758:Viral Core Proteins / Q000032:analysis; D014779:Virus Replication
D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D014758:Viral Core Proteins; D003513:Cycloheximide; D012194:RNA-Directed DNA Polymerase