Immunologic and structural relatedness of the integrin beta 7 complex and the human intraepithelial lymphocyte antigen HML-1.
We recently cloned the newest human integrin beta subunit, termed beta 7, from a cDNA library constructed from SEA-activated T lymphocytes. In this communication, we report on the structure of the human integrin beta 7 protein complex determined using a rabbit anti-beta 7 peptide antibody raised to an N-terminal 22 amino acid residue sequence deduced from the human beta 7 subunit cDNA. The beta 7 subunit (Mr 116,000) expressed on PHA lymphoblasts associates with a single major alpha subunit (alpha H) that is distinct from the prominent T cell marker, integrin alpha 4. The alpha H subunit (Mr 180,000 nonreduced) displays a distinctive shift in size on reduction to an apparent Mr of 150,000. We show that these structural properties of the integrin beta 7 complex are shared with the cell surface antigen HML-1 found highly expressed on T cells which populate the intestinal epithelium and are proposed to be involved in mucosal immunity. Sequential immunoprecipitation and Western blotting demonstrate identity or close homology between the alpha H beta 7 and HML-1 proteins.
Krissansen
G W
GW
Department of Molecular Medicine, School of Medicine, University of Auckland, New Zealand.
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Prestidge
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Hollander
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Jiang
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Jenkins
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D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor; D015153:Blotting, Western; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007150:Immunohistochemistry; D039641:Integrin beta Chains; D016023:Integrins / Q000235:genetics / Q000276:immunology*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins / Q000276:immunology
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D039641:Integrin beta Chains; D016023:Integrins; D010835:Phytohemagglutinins; C070709:integrin alphaEbeta7; C091368:integrin beta7; D004247:DNA