title: Nitrogen catabolite repression of arginase (CAR1) expression in Saccharomyces cerevisiae is derived from regulated inducer exclusion.
abstract: Expression of the Saccharomyces cerevisiae arginase (CAR1) gene is regulated by induction and nitrogen catabolite repression (NCR). Arginine was demonstrated to be the native inducer. CAR1 sensitivity to NCR has long been accepted to be accomplished through a negative control mechanism, and cis-acting sites for it have been hypothesized. In search of this negatively acting site, we discovered that CAR1 sensitivity to NCR derives from regulated inducer (arginine) exclusion. The route of catabolic entry of arginine into the cell, the general amino acid permease (GAP1), is sensitive to NCR. However, CAR1 expression in the presence of sufficient intracellular arginine is NCR insensitive.
authors.affiliation: Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
authors.forename: T G
authors.initials: TG
authors.lastname: Cooper
authors.forename: L
authors.initials: L
authors.lastname: Kovari
authors.forename: R A
authors.initials: RA
authors.lastname: Sumrada
authors.forename: H D
authors.initials: HD
authors.lastname: Park
authors.forename: R M
authors.initials: RM
authors.lastname: Luche
authors.forename: I
authors.initials: I
authors.lastname: Kovari
mesh_terms: D001119:Arginase / Q000235:genetics*; D001120:Arginine / Q000378:metabolism*; D001216:Asparagine / Q000378:metabolism; D001692:Biological Transport; D004252:DNA Mutational Analysis; D004790:Enzyme Induction / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D007763:Lac Operon; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D011993:Recombinant Fusion Proteins; D001216:Asparagine; D001120:Arginine; D001616:beta-Galactosidase; D001119:Arginase