[An active 2.5S RNA-polysaccharide complex].
The information on the initial step of enzymatic reaction of 2.5S RNA isolated from muscle 1,4-alpha-glucane branching enzyme (EC 2.4.1.18), isomerase amylose, has been reported for the first time. The 2.5S RNA-polysaccharide (starch) complex was isolated and partly characterized. It was shown that the complex retains its integrity during precipitation with ethanol, gel-filtration on a Biogel P-150 column and electrophoresis but dissociates into RNA and starch upon fractionation on a DEAE-52 cellulose column. Treatment of the original preparation with RNAase fully reflects the complex formation: with a decrease in the RNA content in the original preparation that of the synthesized complex diminishes. The RNA-polysaccharide complex exhibits the properties of the branching enzyme; its enzymatic activity markedly exceeds that of the free RNA.
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D015061:1,4-alpha-Glucan Branching Enzyme / Q000378:metabolism*; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D006868:Hydrolysis; D009132:Muscles / Q000201:enzymology; D011134:Polysaccharides / Q000302:isolation & purification / Q000378:metabolism*; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits
D011134:Polysaccharides; D012313:RNA; D015061:1,4-alpha-Glucan Branching Enzyme
Aktivnyĭ kompleks 2.5S RNK-polisakharid.