Flow cytometric reticulocyte analysis using thiazole orange; clinical experience and technical limitations.
Flow cytometric (FCM) reticulocyte analysis using thiazole orange (TO) is becoming an increasingly popular method for routine quantification of reticulocytes. The methodology is accurate, cost-effective and shows a high correlation with manual techniques. We describe our experience with the clinical application of FCM reticulocyte analysis in a general hospital setting over a 20-month period with special emphasis on technical limitations.
Chin-Yee
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I
University of Western Ontario, Department of Hematology/Blood Bank, Victoria Hospital Corporation, London, Canada.
Keeney
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M
Lohmann
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RC
D000970:Antineoplastic Agents / Q000627:therapeutic use; D052160:Benzothiazoles; D016026:Bone Marrow Transplantation / Q000473:pathology; D003131:Combined Modality Therapy; D004906:Erythrocyte Count / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D011804:Quinolines; D012313:RNA / Q000097:blood*; D012016:Reference Values; D015203:Reproducibility of Results; D012156:Reticulocytes / Q000166:cytology*; D013844:Thiazoles
D000970:Antineoplastic Agents; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D011804:Quinolines; D013844:Thiazoles; C051000:thiazole orange; D012313:RNA