A human alpha-fetoprotein enhancer-binding protein, ATBF1, contains four homeodomains and seventeen zinc fingers.
We have isolated a full-length cDNA encoding a protein (ATBF1) that binds to an AT-rich motif in the human alpha-fetoprotein gene enhancer. The amino acid sequence deduced from the cDNA revealed that this is the largest DNA-binding protein (306 kDa) known to date, containing four homeodomains, 17 zinc finger motifs, and a number of segments potentially involved in transcriptional regulation. Although the exact function of this protein has not been determined, these structural features suggest that ATBF1 plays a transcriptional regulatory role.
Morinaga
T
T
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Japan.
Yasuda
H
H
Hashimoto
T
T
Higashio
K
K
Tamaoki
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004742:Enhancer Elements, Genetic; D018398:Homeodomain Proteins; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured; D016335:Zinc Fingers; D000509:alpha-Fetoproteins / Q000235:genetics*
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D000509:alpha-Fetoproteins; C071660:ZFHX3 protein, human; D004247:DNA