Chemical synthesis of branched RNAs: a new method for construction of synthetic units for branched RNAs by use of 2'-phosphorylation on the basis of steric control.
A 3',5'-unprotected 2'-O-bis(tert-butoxy)-phosphinyl-6-N-benzoyladenosine derivative was prepared as a key intermediate for the synthesis of branched RNAs, and used for construction of building units from which chain elongation in any of 2'-, 3'-, and 5'-directions would be possible. From this synthetic intermediate, 2'-phosphorylated ApC dimer was also synthesized.
Sekine
M
M
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Iimura
S
S
Mori
T
T
Tsuruoka
H
H
Furusawa
K
K
D001483:Base Sequence; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D010766:Phosphorylation; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
D009843:Oligoribonucleotides; D012313:RNA