title: Quantification and partial characterisation of regulatory peptides in the liver fluke, Fasciola hepatica, from different mammalian hosts.
abstract: 1. Extracts of the liver fluke, Fasciola hepatica from three different hosts (cow, sheep, rat) have been subjected to radioimmunoassay using antisera to 6 mammalian regulatory peptides. 2. Immunoreactivity was measured to pancreatic polypeptide, substance P, peptide histidine isoleucine and gastrin-releasing peptide. Levels of each peptide varied considerably in flukes from different hosts. 3. Reverse-phase HPLC of rat and sheep fluke extracts revealed three molecular forms of tachykinin immunoreactivity and single peaks of pancreatic polypeptide and peptide histidine isoleucine immunoreactivity. No GRP-immunoreactivity was detected by RIA of HPLC fractions.
authors.affiliation: School of Biology and Biochemistry, Queen's University, Belfast, Northern Ireland.
authors.forename: R M
authors.initials: RM
authors.lastname: Magee
authors.forename: I
authors.initials: I
authors.lastname: Fairweather
authors.forename: C
authors.initials: C
authors.lastname: Shaw
authors.forename: J M
authors.initials: JM
authors.lastname: McKillop
authors.forename: W I
authors.initials: WI
authors.lastname: Montgomery
authors.forename: C F
authors.initials: CF
authors.lastname: Johnston
authors.forename: D W
authors.initials: DW
authors.lastname: Halton
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D002851:Chromatography, High Pressure Liquid; D005210:Fasciola hepatica / Q000032:analysis*; D005211:Fascioliasis / Q000469:parasitology / Q000662:veterinary*; D019886:Gastrin-Releasing Peptide; D009479:Neuropeptides / Q000032:analysis*; D010191:Pancreatic Polypeptide / Q000032:analysis; D010451:Peptide PHI / Q000032:analysis; D010455:Peptides / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*; D013373:Substance P / Q000032:analysis; D015320:Tachykinins / Q000032:analysis
chemical_list: D009479:Neuropeptides; D010451:Peptide PHI; D010455:Peptides; D015320:Tachykinins; D013373:Substance P; D010191:Pancreatic Polypeptide; D019886:Gastrin-Releasing Peptide