title: The ATPase-staining of Langerhans cells in previously stored skin tissues.
abstract: The ATPase-staining of Langerhans cells is usually done on fresh, unfixed biopsy specimens. In the present study, we attempted to stain these cells in previously stored tissues of guinea pig skin. The ATPase activity disappeared after freezing or preservation in formalin or ethanol. In contrast, tissue specimens which had been stored in physiological saline at 4 degrees C stained as well as fresh cells even after 14 days.
authors.affiliation: Department of Dermatology, Kansai Medical University, Osaka, Japan.
authors.forename: Y K
authors.initials: YK
authors.lastname: Kim
authors.forename: H
authors.initials: H
authors.lastname: Miyauchi
authors.forename: T
authors.initials: T
authors.lastname: Horio
authors.forename: Y
authors.initials: Y
authors.lastname: Asada
mesh_terms: D000251:Adenosine Triphosphatases; D000818:Animals; D000078404:Epidermal Cells; D000431:Ethanol; D005404:Fixatives; D005557:Formaldehyde; D005615:Freezing; D006168:Guinea Pigs; D007801:Langerhans Cells / Q000166:cytology*; D012867:Skin / Q000166:cytology*; D012965:Sodium Chloride; D013194:Staining and Labeling; D014021:Tissue Preservation
chemical_list: D005404:Fixatives; D005557:Formaldehyde; D000431:Ethanol; D012965:Sodium Chloride; D000251:Adenosine Triphosphatases