[125I]SCH 23982, a new tool for rapid visualization of dopaminergic neurons in lower vertebrate retinas.
Incubation of turtle or Xenopus retinas in 0.1 nM [125I]SCH 23982, a dopamine D1 receptor antagonist for 30-45 min and subsequent fixation in paraformaldehyde/glutaraldehyde resulted in strong blue formaldehyde-induced fluorescence of inner retinal neurons. On the basis of their morphological features, the labeled cells were classified as dopaminergic cells, an identification which was confirmed by double-labeling experiments using an antiserum against tyrosine hydroxylase. The whole experiment can be conducted in less than 2 h (whole mount), the label is very stable and allows the use of high-magnification objectives for detailed morphological investigation of dopaminergic retinal neurons.
Schütte
M
M
Department of Ophthalmology, NYU Medical Center, NY 10016.
D000818:Animals; D001552:Benzazepines / Q000031:analogs & derivatives*; D004298:Dopamine / Q000502:physiology*; D018492:Dopamine Antagonists; D007150:Immunohistochemistry; D009474:Neurons / Q000648:ultrastructure*; D012160:Retina / Q000166:cytology*; D013194:Staining and Labeling; D014426:Turtles / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology; D014982:Xenopus laevis / Q000502:physiology
C049558:8-iodo-2,3,4,5-tetrahydro-3-methyl-5-phenyl-1H-3-benzazepine-7-ol; D001552:Benzazepines; D018492:Dopamine Antagonists; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine