title: In vitro analysis of the origin and maintenance of O-2Aadult progenitor cells.
abstract: We have been studying the differing characteristics of oligodendrocyte-type-2 astrocyte (O-2A) progenitors isolated from optic nerves of perinatal and adult rats. These two cell types display striking differences in their in vitro phenotypes. In addition, the O-2Aperinatal progenitor population appears to have a limited life-span in vivo, while O-2Aadult progenitors appear to be maintained throughout life. O-2Aperinatal progenitors seem to have largely disappeared from the optic nerve by 1 mo after birth, and are not detectable in cultures derived from optic nerves of adult rats. In contrast, O-2Aadult progenitors can first be isolated from optic nerves of 7-d-old rats and are still present in optic nerves of 1-yr-old rats. These observations raise two questions: (a) From what source do O-2Aadult progenitors originate; and (b) how is the O-2Aadult progenitor population maintained in the nerve throughout life? We now provide in vitro evidence indicating that O-2Aadult progenitors are derived directly from a subpopulation of O-2Aperinatal progenitors. We also provide evidence indicating that O-2Aadult progenitors are capable of prolonged self renewal in vitro. In addition, our data suggests that the in vitro generation of oligodendrocytes from O-2Aadult progenitors occurs primarily through asymmetric division and differentiation, in contrast with the self-extinguishing pattern of symmetric division and differentiation displayed by O-2Aperinatal progenitors in vitro. We suggest that O-2Aadult progenitors express at least some properties of stem cells and thus may be able to support the generation of both differentiated progeny cells as well as their own continued replenishment throughout adult life.
authors.affiliation: Department of Clinical Neurology, Institute of Neurology, Queen Square, London, England.
authors.forename: D
authors.initials: D
authors.lastname: Wren
authors.forename: G
authors.initials: G
authors.lastname: Wolswijk
authors.forename: M
authors.initials: M
authors.lastname: Noble
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology*; D002453:Cell Cycle; D002454:Cell Differentiation; D002455:Cell Division; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development*; D007700:Kinetics; D009836:Oligodendroglia / Q000166:cytology*; D009900:Optic Nerve / Q000166:cytology / Q000254:growth & development*; D051381:Rats; D013997:Time Factors