title: Molecular characterization of the mouse mannose-binding proteins. The mannose-binding protein A but not C is an acute phase reactant.
abstract: Mannose-binding proteins play a role in first line host defense against a variety of pathogens. We report the molecular cloning of two mouse mannose-binding proteins designated A and C based on their close identity with their rat homologues. The deduced amino acid sequence of the mouse mannose-binding proteins, as with rat and the human forms, have an NH2 terminus that is rich in cysteine that stabilizes a collagen alpha helix followed by a carboxyl- terminal carbohydrate binding domain. We further show that the mouse mannose-binding protein A mRNA, as with the human, is induced like the acute phase reactant serum amyloid P protein, yet the expression of mouse mannose-binding protein C mRNA is not regulated above its low baseline level. The expression of both mannose-binding proteins A and C mRNA is restricted to the liver under basal and stress conditions.
authors.affiliation: Division of Hematology/Oncology, Children's Hospital, Boston, MA.
authors.forename: K
authors.initials: K
authors.lastname: Sastry
authors.forename: K
authors.initials: K
authors.lastname: Zahedi
authors.forename: J M
authors.initials: JM
authors.lastname: Lelias
authors.forename: A S
authors.initials: AS
authors.lastname: Whitehead
authors.forename: R A
authors.initials: RA
authors.lastname: Ezekowitz
mesh_terms: D000209:Acute-Phase Proteins / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008099:Liver / Q000502:physiology; D037601:Mannose-Binding Lectin; D037241:Mannose-Binding Lectins; D051379:Mice; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics; D013997:Time Factors
chemical_list: D000209:Acute-Phase Proteins; D002352:Carrier Proteins; C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin; D037241:Mannose-Binding Lectins; C445639:Mbl2 protein, mouse; C445599:Mbl2 protein, rat; D009841:Oligonucleotides; D012333:RNA, Messenger; C069858:mannose binding protein A; D004247:DNA