Effects of adenosine and its derivatives on cultured myocardial cells and detection of adenosine receptors in rat.
Adenosine and its derivatives 1 nmol.L-1 -0.1 mmol.L-1 elicited negative chronotropic effect on cultured rat myocardial cells. The respective IC50 were: L-PIA 85 +/- 10 greater than NECA 160 +/- 64 greater than adenosine 361 +/- 41 nmol.L-1. The negative chronotropic effect was antagonized by IBMX. [3H]Adenosine bound to myocardial cells of rat in a saturable way with a Kd of 238 nmol.L-1 and a Bmax of 7.6 pmol/10(8) cells. The binding of [3H]adenosine was competitively antagonized by adenosine, L-PIA, NECA, and IBMX. These results indicated the existence of adenosine receptor in rat myocardial cells.
Wei
X D
XD
Department of Biology, Sun Yat-Sen University of Medical Science, Guangzhou, China.
Cai
S D
SD
Qiu
Y G
YG
You
S X
SX
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology*; D019830:Adenosine-5'-(N-ethylcarboxamide); D000818:Animals; D001667:Binding, Competitive; D002478:Cells, Cultured; D003864:Depression, Chemical; D005260:Female; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011983:Receptors, Purinergic / Q000032:analysis*
D011983:Receptors, Purinergic; D010660:Phenylisopropyladenosine; D019830:Adenosine-5'-(N-ethylcarboxamide); D000241:Adenosine; D015056:1-Methyl-3-isobutylxanthine