title: [Cytoskeletal changes in A-431 cells under the action of epidermal growth factor].
abstract: By the use of rhodamine-phalloidin, the distribution of actin in A-431 cells during the action of epidermal growth factor (EGF) has been studied. Changes in the pattern of staining are observed in 30-60 s after addition of the EGF. Microvilli and wrinkles are created on the cell surface. Following a 5-10 min action of EGF, rhodamine-phalloidin stained intensely ruffles and cell borders. After 60 min, the ruffling of cell surface disappeared, and actin was seen concentrating on the cell borders only. Electron microscopy of the EGF-treated A-431 cells lysed by Triton X-100 also revealed some vigorous fibrillar bunches on the cell edges.
authors.forename: V I
authors.initials: VI
authors.lastname: Skopicheva
authors.forename: V G
authors.initials: VG
authors.lastname: Skopichev
authors.forename: N A
authors.initials: NA
authors.lastname: Vinogradova
authors.forename: L V
authors.initials: LV
authors.lastname: Teslenko
authors.forename: N N
authors.initials: NN
authors.lastname: Nikol'skiĭ
mesh_terms: D000199:Actins / Q000187:drug effects / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000648:ultrastructure; D002460:Cell Line; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D000199:Actins; D003571:Cytochalasin B; D014747:Vinblastine; D004815:Epidermal Growth Factor
vernacular_title: Izmenenie tsitoskeleta kletok A-431 pri deĭstvii épidermal'nogo faktora rosta.