The influence of properties of packing materials upon the recovery of biological substances isolated from urine by solid-phase extraction.
A series of packing materials with alkyl phase chemically bonded to silica gels of various porosity have been prepared. These packings have been used to isolate the test substances 5-hydroxyindole-3-acetic acid (5-HIAA) and serotonin (5-HT) from urine. The influence of the support porosity, structure of chemically bonded phase, length of alkyl chain, and coverage density on the recovery of isolated substances was studied.
Buszewski
B
B
Department of Chemical Physics, Faculty of Chemistry Maria Curie Sklodowska University, Lublin, Poland.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine; D011868:Radioisotopes; D012701:Serotonin / Q000652:urine; D012821:Silanes; D012825:Silicon; D014556:Urine / Q000737:chemistry*
D011868:Radioisotopes; D012821:Silanes; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D012825:Silicon