Characterization of a 70Z/3 pre-B cell derived macrophage clone. Differential expression of Hox family genes.
An adherent cell line was established from the much studied pre-B cell line 70Z/3. The adherent cell line had the morphological features of a macrophage and stained positive for non-specific esterase. In addition, this line expressed high levels of RNA for the macrophage specific enzyme, lysozyme. Although the 70Z/3 macrophage variant has lost the ability to respond to lipopolysaccharide and interferon-gamma by expressing RNA transcripts for immunoglobulin light chain, it exhibited a new response characterized by increased levels of I-A RNA transcripts. Both the pre-B and macrophage variants also expressed RNA transcripts which hybridize to a Hox 2.3 probe. In contrast, transcripts which hybridize to Hox 1.1, 2.1, and 6.1 probes are expressed in the pre-B line but could not be detected in the macrophage.
Hara
H
H
Ontario Cancer Institute, Toronto, Canada.
Sam
M
M
Maki
R A
RA
Wu
G E
GE
Paige
C J
CJ
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000201:enzymology / Q000276:immunology; D002448:Cell Adhesion; D002999:Clone Cells / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D008264:Macrophages / Q000166:cytology* / Q000201:enzymology / Q000276:immunology; D015335:Molecular Probes; D012313:RNA / Q000235:genetics; D014158:Transcription, Genetic
D015335:Molecular Probes; D012313:RNA