title: Isolation of two forms of decay-accelerating factor (DAF) from human urine.
abstract: Decay-accelerating factor (DAF) was purified from human pooled urine by conventional techniques. The urine DAF was separated into two peaks, pool I and pool II, by gel chromatography. DAF-U1 was isolated from pool I by hydrophobic chromatography, and DAF-U2 from pool II by anti-DAF IgG column. The specific activities of DAF-U1 and DAF-U2 to decay membrane-phase C5 convertase were about 3% and 70% of membrane form DAF, respectively. However, both urine DAFs revealed a similar activity to each other and slightly higher activity than that of membrane form DAF in decay-accelerating fluid-phase C3 convertase of the alternative pathway.
authors.affiliation: Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: Y
authors.initials: Y
authors.lastname: Sugita
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: N H
authors.initials: NH
authors.lastname: Choi
authors.forename: T
authors.initials: T
authors.lastname: Tobe
authors.forename: M
authors.initials: M
authors.lastname: Tomita
mesh_terms: D000595:Amino Acid Sequence; D018958:CD55 Antigens; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D050577:Complement C3-C5 Convertases / Q000037:antagonists & inhibitors; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine; D008969:Molecular Sequence Data
chemical_list: D018958:CD55 Antigens; D008565:Membrane Proteins; D050577:Complement C3-C5 Convertases