title: Measurement of beta 2-microglobulin, retinol-binding protein, alpha 1-microglobulin and urine protein 1 in healthy children using enzyme-linked immunosorbent assay.
abstract: Enzyme-linked immunosorbent assays (ELISA) have been developed for the measurement of beta 2-microglobulin (B2M), retinol-binding protein (RBP), alpha 1-microglobulin (A1M) and urine protein 1 (UP1) in children. Results from random urine samples in 43 children (31 for B2M) are, when corrected for urine creatinine (geometric mean (range)): B2M 9.8 (6.0-40.7) micrograms/mmol, RBP 8.1 (less than 1-24.5) micrograms/mmol, A1M 0.4 (0.1-2.2) mg/mmol and UP1 17.8 (less than 2-309.4) micrograms/mmol. Fractional excretions (FE) in 23 children (14 for B2M) are (geometric mean (range)): FEB2M 0.04% (0.02-0.10%) and FEUP1 0.10% (0.01-1.21%). Results in overnight urine collections are also presented. Our results extend existing data for normal ranges in adults to include children and provide data on UP1 concentrations.
authors.affiliation: Evelina Children's Unit, Guy's Hospital, United Medical and Dental School, London, UK.
authors.forename: P A
authors.initials: PA
authors.lastname: Tomlinson
authors.forename: R N
authors.initials: RN
authors.lastname: Dalton
authors.forename: C
authors.initials: C
authors.lastname: Turner
authors.forename: C
authors.initials: C
authors.lastname: Chantler
mesh_terms: D000293:Adolescent; D000510:Alpha-Globulins / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D011506:Proteins / Q000032:analysis*; D012016:Reference Values; D012177:Retinol-Binding Proteins / Q000652:urine*; D012727:Sex Characteristics; D014598:Uteroglobin; D001613:beta 2-Microglobulin / Q000032:analysis / Q000652:urine*
chemical_list: D000510:Alpha-Globulins; D011506:Proteins; D012177:Retinol-Binding Proteins; C556389:SCGB1A1 protein, human; C016001:alpha-1-microglobulin; D001613:beta 2-Microglobulin; D014598:Uteroglobin