Isolation and characterization of fatty acid binding protein in the liver of the nurse shark, Ginglymostoma cirratum.
1. A 14.5 kDa fatty acid binding protein was isolated from the liver of the nurse shark, Ginglymostoma cirratum. 2. Purified shark liver FABP (pI = 5.4) bound oleic acid at a single site with an affinity similar to that of mammalian FABP. 3. The apparent size, pI and amino acid composition of shark liver FABP indicate a close structural relationship between this protein and mammalian heart FABP.
Bass
N M
NM
Department of Medicine, Liver Center, University of California, San Francisco 94143.
Manning
J A
JA
Luer
C A
CA
D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003600:Cytosol / Q000737:chemistry; D003911:Dextrans / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D008099:Liver / Q000737:chemistry*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D051381:Rats; D011919:Rats, Inbred Strains / Q000378:metabolism*; D012754:Sharks / Q000378:metabolism*
D002352:Carrier Proteins; D003911:Dextrans; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; C010599:lipidex