Histological detection of c-myb and c-myc proto-oncogene expression in infiltrating cells in cutaneous lupus erythematosus-like lesions of MRL/l mice by in situ hybridization.
A relationship between lymphocytic activation and the overexpression of proto-oncogenes such as c-myb or c-myc has been demonstrated in human autoimmune disease. In autoimmune-prone MRL/l mice, which spontaneously develop lupus erythematosus (LE)-like lesions on the back, increased expression of myb RNA has been found in the lymphoid organs. We detected the overexpression of c-myb and c-myc proto-oncogenes in infiltrating cells in the cutaneous lesions of MRL/l mice by using in situ hybridization. No specific hybridization signals of either of the probes used were seen in the nonlesional skin of MRL/l mice or in the apparently normal skin of aged MRL/n and young MRL/l mice. These results suggest that the increased expression of myb and myc proto-oncogenes in the cutaneous LE-like lesions of MRL/l mice is related to a state of activation in the infiltrating cells and is involved in the development of these lesions.
Kitajima
T
T
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
Furukawa
F
F
Kanauchi
H
H
Imamura
S
S
Ogawa
K
K
Sugiyama
T
T
D000818:Animals; D016259:Genes, myc; D008178:Lupus Erythematosus, Cutaneous / Q000235:genetics*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D000090063:Proto-Oncogene Mas; D012867:Skin / Q000473:pathology*