Antipeptide antibodies against the pNR-2 oestrogen-regulated protein of human breast cancer cells and detection of pNR-2 expression in normal tissues by immunohistochemistry.
Five peptides, corresponding to regions of the predicted protein sequence of the oestrogen-regulated pNR-2 protein which is expressed in oestrogen-responsive human breast cancer cells, were synthesized. Two peptides were immunogenic in rabbits and antisera against one peptide reacted with the pNR-2 protein in sections of formalin-fixed, paraffin-embedded breast tumour. There was a significant correlation between the extent of pNR-2 protein expression detected by immunohistochemistry and pNR-2 mRNA levels determined by hybridization with a cDNA probe in a series of primary breast tumours. pNR-2 expression was assessed immunohistochemically in a panel of normal tissues. Expression was detected in normal breast, small intestine, and stomach (body and antrum).
Piggott
N H
NH
Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne.
Henry
J A
JA
May
F E
FE
Westley
B R
BR
D000595:Amino Acid Sequence; D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology; D001483:Base Sequence; D001940:Breast / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology*; D015342:DNA Probes; D004967:Estrogens / Q000378:metabolism; D006801:Humans; D007106:Immune Sera; D007421:Intestine, Small / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis* / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D013194:Staining and Labeling; D013270:Stomach / Q000276:immunology; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins
D000912:Antibodies, Neoplasm; D015342:DNA Probes; D004967:Estrogens; D007106:Immune Sera; D009363:Neoplasm Proteins; D010455:Peptides; D011506:Proteins; D012333:RNA, Messenger; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins