[Determination of the esterase activity of serine proteinases using synthetic substrates].
The authors suggest a highly sensitive rapid and simple method for measuring esterase activities of bovine pancreatic chymotrypsin, human neutrophilic cathepsin D, and elastase, and of human blood serum chymotrypsin-like esterase and elastase-like esterase activities, with fluorogenic synthetic ethers, amino acid derivatives, employed as substrates: N-benzoxycarbonylphenylalanine 4-methylumbelliferyl ester (Z-Phe-OMC) and tret-butyloxycarbonyl-1-alanine 4-methylumbelliferyl ester (BOC-Ala-OMC).
Kraeva
L N
LN
Pozdnev
V F
VF
Kokriakov
V N
VN
Usova
A A
AA
D000818:Animals; D002417:Cattle; D004950:Esterases / Q000378:metabolism*; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives; D012697:Serine Endopeptidases / Q000378:metabolism*
C070487:tert-butyloxycarbonyl-1-alanine 4-methylumbelliferyl ester; D006923:Hymecromone; C047960:benzyloxycarbonylphenylalanine 4-methylcoumaryl-7-ester; D004950:Esterases; D012697:Serine Endopeptidases
Opredelenie ésteraznoĭ aktivnosti serinovykh proteinaz s ispol'zovaniem sinteticheskikh substratov.