Two methods for flat embedding sections in LR white cut from paraffin blocks.
Two simple methods are described for flat embedding of sections cut from paraffin blocks of brain tissue in the hydrophilic resin LR White. The ability of fresh resin to polymerize when added to already cured resin is exploited. In the first method, a tissue section with a drop of LR White is pressed to the bottom of a gelatin capsule using a blank block. In the second method, the section in a drop of resin is sandwiched between the sawed halves of a blank block. After curing, thin sections containing large areas of tissue can be collected and processed for immunocytochemistry.
Migheli
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2nd Neurologic Clinic, University of Turin, Italy.
Attanasio
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D000180:Acrylic Resins; D000690:Amyotrophic Lateral Sclerosis / Q000473:pathology; D001921:Brain / Q000033:anatomy & histology*; D001932:Brain Neoplasms / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D010232:Paraffin; D010870:Pineal Gland / Q000473:pathology; D013116:Spinal Cord / Q000473:pathology; D013194:Staining and Labeling
D000180:Acrylic Resins; D010232:Paraffin; C048707:LR white