The RNA component of RNase P from the archaebacterium Haloferax volcanii.
RNase P, an endoribonuclease responsible for generating the mature 5' termini of tRNA precursors, is composed of both RNA and protein. It has been demonstrated that the eubacterial RNase P RNA will, under the appropriate reaction conditions, exhibit catalytic activity in vitro. Evidence has not been obtained for catalytic activity by the RNAs of eukaryotic RNase P enzymes. Using a cDNA probe prepared from RNA copurifying with RNase P activity from the archaebacterium Haloferax volcanii, we have characterized the gene encoding the RNase P RNA. The proposed transcript from this gene can assume a structure resembling the eubacterial RNase P RNA and includes many of the highly conserved sequences of these RNAs. This RNA was incapable of cleaving pre-tRNA substrates in the absence of protein under a variety of in vitro conditions. Catalytic activity was observed when this RNA was combined with the protein subunit of the Bacillus subtilis RNase P complex. Similarities among the archaebacterial, eubacterial, and eukaryotic RNase P RNA sequences and structures are discussed.
Nieuwlandt
D T
DT
Department of Microbiology, Ohio State University, Columbus 43210.
Haas
E S
ES
Daniels
C J
CJ
D001105:Archaea / Q000201:enzymology*; D001412:Bacillus subtilis / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000235:genetics; D004722:Endoribonucleases / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000235:genetics*; D012366:RNA, Transfer, Val / Q000235:genetics; D043262:Ribonuclease P; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012366:RNA, Transfer, Val; D004722:Endoribonucleases; D043262:Ribonuclease P