Detection of common polysaccharide antigen of Pseudomonas aeruginosa with O-antiserum to Pseudomonas cerasi.
The identity of the structures of common polysaccharide antigen (CPA) of Pseudomonas aeruginosa and O-antigen of Pseudomonas cerasi was used for immunochemical study of polysaccharide antigens of seven immunotypes (IT) of P. aeruginosa. ELISA performed with O-antiserum to P. cerasi showed that CPA is present in all seven ITs in different amounts. In SDS-PAGE this antigen behaves as a lipopolysaccharide (LPS) and is detected by immunoblotting technique in five of seven ITs.
Kocharova
N A
NA
N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow.
Knirel
Y A
YA
Romanowska
A
A
Romanowska
E
E
Makarenko
T A
TA
Edvabnaya
L S
LS
Stanislavsky
E S
ES
Zdorovenko
G M
GM
Yakovleva
L M
LM
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera; D008969:Molecular Sequence Data; D019081:O Antigens; D011549:Pseudomonas / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011817:Rabbits; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera; D019081:O Antigens