Protein binding to the interferon response enhancer correlates with interferon induction of 2'-5'-oligoadenylate synthetase in normal and interferon-resistant Friend cells.
The induction of transcription of the 2'-5'-oligoadenylate (2-5A) synthetase gene by type I (alpha/beta) and type II (gamma) interferons (IFNs) has been studied in wild-type (w.t.) and IFN-resistant Friend leukemia cells (FLC). Following IFN treatment, new complexes are formed in vitro between the IFN-responsive sequence (IRS) of the 2-5A synthetase gene and cellular proteins. Within minutes after IFN-alpha/beta addition to w.t. FLC, an IRS-protein complex, designated F1, is detected, as already observed in several human cell lines. In response to IFN-gamma, a novel complex, designated Fg, is observed in w.t. FLC. The Fg complex appears within 3 h, while an F1-like complex is faintly visible 10 to 24 h later. In the IFN-alpha/beta-resistant FLC, IFN-gamma induces only the Fg complex and fails to induce F1. Fg formation is correlated with the IFN-gamma-induced transcription of the 2-5A synthetase gene and the appearance of the corresponding enzymatic activity in both w.t. and IFN-alpha/beta-resistant FLC. These findings suggest that F1 and Fg represent two distinct effector complexes by which type I and type II IFNs, respectively, induce 2-5A synthetase.
Coccia
E M
EM
Laboratorio di Virologia, Istituto Superiore di Sanità, Rome, Italy.
Vaiman
D
D
Raber
J
J
Marziali
G
G
Fiorucci
G
G
Orsatti
R
R
Cohen
B
B
Nissim
N
N
Romeo
G
G
Affabris
E
E
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D004742:Enhancer Elements, Genetic; D004790:Enzyme Induction; D005622:Friend murine leukemia virus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D007370:Interferon Type I / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute / Q000382:microbiology; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis*; D014158:Transcription, Genetic
D007370:Interferon Type I; D012333:RNA, Messenger; D007371:Interferon-gamma; D007372:Interferons; D003513:Cycloheximide; D015088:2',5'-Oligoadenylate Synthetase