A novel proline-rich protein from wheat.
A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich proteins. The WPRP1 cDNA clone was used to determine the copy number and chromosomal location of the WPRP1 gene by restriction fragment length polymorphism analysis of wheat inbred lines. Although WPRP1 is encoded by a single-copy gene it is also a representative of a larger family of related sequences. RNA gel blot analysis showed that expression of WPRP1 is highest in rapidly growing tissue which together with its amino acid composition suggests a structural role for the encoded protein.
Raines
C A
CA
Cambridge Laboratory, JI Centre for Plant Science Research, Norwich, UK.
Lloyd
J C
JC
Chao
S M
SM
John
U P
UP
Murphy
G J
GJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011061:Poly A / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014908:Triticum / Q000235:genetics*
D010940:Plant Proteins; D012333:RNA, Messenger; C070314:WPRP1 protein, wheat; D011061:Poly A; D012313:RNA; D004247:DNA