title: Rice protein 16KD--a major allergen in rice grain extract.
abstract: The allergenic activity of Rice protein 16 KD (RP16KD) isolated from water soluble rice proteins was examined by radioallergosorbent test (RAST), RAST inhibition and histamine release assay. All of the 31 sera which showed positive RAST values for rice grain extract were positive for RP16KD RAST. Furthermore, there was a significant correlation (r = 0.56, p less than 0.01) between these RAST values. PR16KD effectively inhibited IgE binding to the rice grain extract disc in RAST inhibition assays using 4 sera with positive RAST values for both antigens. In 17 subjects with positive RAST values for rice grain extract, a significant positive correlation (r = 0.53, p less than 0.05) was found between the maximum percent histamine releases from their leukocytes by rice grain extract and RP16KD. These data strongly suggest that RP16KD is one of the major allergens of rice grain.
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine.
authors.forename: A
authors.initials: A
authors.lastname: Urisu
authors.forename: E
authors.initials: E
authors.lastname: Wada
authors.forename: Y
authors.initials: Y
authors.lastname: Kondo
authors.forename: F
authors.initials: F
authors.lastname: Horiba
authors.forename: M
authors.initials: M
authors.lastname: Tsuruta
authors.forename: T
authors.initials: T
authors.lastname: Yasaki
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Masuda
authors.forename: H
authors.initials: H
authors.lastname: Komada
authors.forename: M
authors.initials: M
authors.lastname: Yamada
mesh_terms: D000485:Allergens / Q000276:immunology*; D004044:Dietary Proteins / Q000276:immunology*; D006636:Histamine Release; D006801:Humans; D008970:Molecular Weight; D012275:Oryza / Q000276:immunology*; D010936:Plant Extracts / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology*; D011852:Radioallergosorbent Test
chemical_list: D000485:Allergens; D004044:Dietary Proteins; D010936:Plant Extracts; D010940:Plant Proteins