Demonstration of endogenous lectins in synovial tissue.
We have recently shown that synoviocytes and extracellular matrices exhibit distinct patterns of carbohydrate expression. Their biological relevance is however not known. The purpose of the present study was to find out whether human synovial tissue would also show a specific receptor pattern for complex sugar molecules. Endogenous lectins were displayed by means of biotinylated neoglycoproteins and sulfated polysaccharides in paraffin-embedded material or cryosections. In addition to certain carbohydrate components that are known to be constituents of the carbohydrate part of cellular glycoconjugates, our panel included heparin and fucoidan, a sulfated fucose. Binding sites were shown using the avidin-peroxidase technique for light microscopy. The results were compared with immunohistochemical methods and enzyme histochemistry. Our study demonstrates that human synovial tissue contains a complex pattern of endogenous lectins depending on the different types of synovitis. The staining method we used in the investigation allows for precise localization of saccharide binding receptors and is therefore believed to be a reliable technique for further phenotypic characterization of synovial cells.
Zschäbitz
A
A
Institute of Anatomy, Johannes Gutenberg-University of Mainz, Germany.
Gabius
H J
HJ
Stofft
E
E
Reichel
W
W
D000911:Antibodies, Monoclonal; D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology; D001706:Biopsy; D050260:Carbohydrate Metabolism; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D010003:Osteoarthritis / Q000378:metabolism / Q000473:pathology; D011956:Receptors, Cell Surface / Q000378:metabolism; D012016:Reference Values; D013194:Staining and Labeling; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology
D000911:Antibodies, Monoclonal; D037102:Lectins; D011956:Receptors, Cell Surface