Demonstration of acid phosphatase activity in antigenic glycoprotein fractions obtained from the culture filtrate of Pseudomonas pseudomallei.
Pseudomonas pseudomallei, the causative microorganism of melioidosis, was grown in Mueller-Hinton liquid medium, and glycoprotein fractions were separated from the culture filtrate by ammonium sulfate precipitation, gel-filtration with Sephadex G-75, and column chromatography with DEAE-cellulose. The fractions revealed acid phosphatase activity, and reacted to the sera from melioidosis patient in gel-diffusion precipitation assay.
Kondo
E
E
Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
Petkanchanapong
V
V
Naigowit
P
P
Kurata
T
T
Kanai
K
K
D000135:Acid Phosphatase / Q000378:metabolism*; D000942:Antigens, Bacterial / Q000032:analysis; D016957:Burkholderia pseudomallei / Q000201:enzymology* / Q000276:immunology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005947:Glucose / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008297:Male; D008554:Melioidosis / Q000382:microbiology
D000942:Antigens, Bacterial; D006023:Glycoproteins; D000135:Acid Phosphatase; D005947:Glucose