Lymphokine secretion and cytotoxic activity of human CD4+ T-cell clones against Bordetella pertussis.
Human CD4+ T-cell clones specific for pertussis toxin and other Bordetella pertussis antigens have been tested for their cytotoxic activity, lymphokine production, and capacity to induce immunoglobulin synthesis. Clones specific for the S1 subunit of pertussis toxin were cytotoxic for autologous Epstein-Barr virus-transformed B cells, which had been pulsed with the native antigen, the recombinant S1 subunit of pertussis toxin, or synthetic peptides derived from the S1 amino acid sequence. The killing of antigen-pulsed target cells was class II restricted. All of the T-cell clones produced mostly interleukin-2 and gamma interferon and assisted allogeneic B cells in the production of immunoglobulins M and G but not immunoglobulin E. The potential in vivo role of the cytotoxic activity of these clones is discussed.
Peppoloni
S
S
Sclavo Research Center, Siena, Italy.
Nencioni
L
L
Di Tommaso
A
A
Tagliabue
A
A
Parronchi
P
P
Romagnani
S
S
Rappuoli
R
R
De Magistris
M T
MT
D001402:B-Lymphocytes; D001886:Bordetella pertussis / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002999:Clone Cells; D000939:Epitopes / Q000032:analysis; D006684:HLA-DR Antigens / Q000276:immunology; D004854:Herpesvirus 4, Human; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis*; D007378:Interleukins / Q000096:biosynthesis*; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000633:toxicity
D000939:Epitopes; D006684:HLA-DR Antigens; D007378:Interleukins; D010566:Virulence Factors, Bordetella; D007073:Immunoglobulin E; D007371:Interferon-gamma; D037342:Pertussis Toxin