Idiotypic characterisation of monoclonal antibodies with restricted epitope specificity for retinal S-antigen.
This paper describes the idiotypic specificities of eight murine monoclonal antibodies directed to three independent epitopes on retinal S-antigen. The antigenic sites recognised by these monoclonal antibodies have previously been localised to a small region near the C-terminal of bovine S-antigen. Xenogeneic, site-related anti-idiotypes prepared against each of the monoclonal antibodies recognised common idiotypes only amongst those monoclonal antibodies which reacted with the same epitope on S-antigen. Two of the three idiotypes were detected in the sera of BALB/c mice but not in two strains of rat immunised with xenogeneic S-antigen and none could be detected in the sera of patients with anti-photoreceptor autoantibodies. Our results demonstrate that the idiotypes of murine monoclonal antibodies to retinal S-antigen exhibit restricted epitope specificity but are species-restricted and imply that the S-antigen lacks a dominant antigenic epitope.
Suleyman
S
S
Department of Immunology, United Medical School, Guy's Hospital, London, United Kingdom.
Kasp
E
E
Ellis
B
B
Banga
J P
JP
Dumonde
D C
DC
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D019393:Arrestin; D001324:Autoantigens / Q000276:immunology*; D001667:Binding, Competitive; D000939:Epitopes / Q000276:immunology*; D005136:Eye Proteins / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D066298:In Vitro Techniques; D012164:Retinal Diseases / Q000276:immunology; D014657:Vasculitis / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D019393:Arrestin; D001324:Autoantigens; D000939:Epitopes; D005136:Eye Proteins; D007130:Immunoglobulin Idiotypes