title: Characterization of new rat anti-mouse IgE monoclonals and their use along with chimeric IgE to further define the site that interacts with Fc epsilon RII and Fc epsilon RI.
abstract: Three rat monoclonal antibodies specific for mouse IgE (C12B9, 23G3, and B1E3) were established by using monoclonal anti-DNP mouse IgE (mIgE) as immunogen. These antibodies, as well as a fourth, (R1E4) were characterized. It was found that one antibody (C12B9) recognizes an allotypic determinant (Igh-7a) found on the C epsilon chain of mIgE. Antibody cross-blocking studies and epitope mapping studies using recombinant mIgE indicated that 3 antibodies (C12B9, R1E4 and 23G3) were directed against the C epsilon 3 domain while one (B1E3) was directed against the C epsilon 4 domain. A highly specific sandwich RIA for mIgE was developed using these antibodies. Use of these monoclonal anti-mIgE antibodies in conjunction with recombinant chimeric mIgE-human IgG1 molecules, demonstrated that the C epsilon 3 domain is important in the binding of mIgE to the murine B cell Fc epsilon RII as well as to the murine mast cell F epsilon RI. The presence of the C epsilon 4 domain influenced the binding of the recombinant IgE to the Fc epsilon RII; in contrast to the C epsilon 4 domain had no effect on binding to the Fc epsilon RI.
authors.affiliation: Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21239.
authors.forename: A D
authors.initials: AD
authors.lastname: Keegan
authors.forename: C
authors.initials: C
authors.lastname: Fratazzi
authors.forename: B
authors.initials: B
authors.lastname: Shopes
authors.forename: B
authors.initials: B
authors.lastname: Baird
authors.forename: D H
authors.initials: DH
authors.lastname: Conrad
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000737:chemistry* / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D001665:Binding Sites; D000939:Epitopes; D006801:Humans; D007073:Immunoglobulin E / Q000737:chemistry / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D011863:Radioimmunoassay; D051381:Rats; D011961:Receptors, Fc / Q000737:chemistry* / Q000378:metabolism; D017455:Receptors, IgE; D011993:Recombinant Fusion Proteins
chemical_list: D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D000939:Epitopes; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017455:Receptors, IgE; D011993:Recombinant Fusion Proteins; D007073:Immunoglobulin E