T cell vaccination does not induce resistance to experimental autoimmune neuritis.
The effectiveness of T cell vaccination was analyzed in experimental autoimmune neuritis (EAN) that can be induced by immunization with bovine P2 protein or a peptide representing the amino acids 53-78 of P2 (P2 53-78). Lewis rats were vaccinated with glutaraldehyde-fixed lymph node cells which had been primed in vivo with P2 protein or P2 53-78 and had been activated in vitro with concanavalin A. Vaccinated animals were not protected from EAN induced by immunization with P2 protein in complete Freund's adjuvant (CFA). In a second set of experiments Lewis rats were vaccinated with irradiated or fixed P2-specific T cell lines of different specificity and neuritogenicity and were subsequently challenged with P2 53-78 in CFA. Likewise, severity of P2 53-78-induced EAN was not different between naive and T line-vaccinated groups. In spleens of vaccinated animals a substantial suppressive activity was demonstrated which was positively correlated with a weak anti-ergotypic response of these spleen cells. The fact that development of actively induced EAN was not prevented or even mitigated by T cell vaccination, in spite of an apparent vaccination-induced response to and on T lymphocytes, suggests that protection from disease is not readily induced in every autoimmune disease model.
Jung
S
S
Department of Neurology, University of Würzburg, F.R.G.
Schluesener
H J
HJ
Toyka
K
K
Hartung
H P
HP
D000818:Animals; D001757:Blastomeres / Q000502:physiology; D004198:Disease Susceptibility; D000939:Epitopes; D005260:Female; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D004676:Myelin Basic Protein / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D018996:Myelin P2 Protein; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology / Q000517:prevention & control*; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000502:physiology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D014611:Vaccination
D000939:Epitopes; D004676:Myelin Basic Protein; D018996:Myelin P2 Protein