The influence of substance P and its fragments on endogenous phosphorylation of synaptosomal membrane protein (synapsin) from cerebral cortex of rat brain.
1. The effects of substance P and its fragments and analogue of a C-terminal fragment on cyclic AMP-dependent phosphorylation of synapsin I in synaptosomal membranes (SM) from cerebral cortex were investigated. 2. SP(I-II) and SP(1-4) at 10(-3) M caused a marked stimulation of synapsin I phosphorylation. 3. A C-terminal fragment of SP (SP6-11) had no effect on phosphorylation of synapsin 1. 4. Analogue of C-terminal fragment [(Tyr8)SP6-11] at 10(-3) M distinctly inhibits phosphorylation of synapsin I. 5. These data suggest that SPI-II and its C- and N-terminal fragments have a modulator function against the phosphorylation of some rat brain proteins.
Hrabec
Z
Z
II Department of Biochemistry, School of Medicine, Lodz, Poland.
Szkudlarek
J
J
Lachowicz
L
L
D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry*; D000242:Cyclic AMP / Q000494:pharmacology; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012697:Serine Endopeptidases / Q000378:metabolism; D013373:Substance P / Q000494:pharmacology*; D016704:Synapsins; D013570:Synaptic Membranes / Q000737:chemistry*
D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D016704:Synapsins; D013373:Substance P; D000242:Cyclic AMP; D011494:Protein Kinases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase