title: X-ray scattering study of hagfish protease inhibitor, a protein structurally related to complement and alpha 2-macroglobulin.
abstract: A protease inhibitor from hagfish blood plasma, homologous to human alpha 2-macroglobulin, has been studied in solution using small-angle X-ray scattering; the radius of gyration, R, was found to be 7.0 nm, the molecular weight 340,000 +/- 20,000, and the largest distance within the molecule, Dmax, 22 nm. When the inhibitor reacts with chymotrypsin, its 1:1 chymotrypsin complex is found to be more compact than the native molecule, R = 6.1 nm. A very similar conformational change is observed after the protein is reacted with methylamine. The data are consistent with models consisting of two equal elliptic cylinders with the same size as the one used as a model for the complement proteins C3 and C4 [cf. Osterberg et al. (1989) Eur. J. Biochem. 183, 507-511]. In the model for the native protein, these cylinders are arranged in an extended form, and in the one for the methylamine derivative (or chymotrypsin complex), they are closer together so that the projection of their elliptic surfaces forms an angle of about 70 degrees. These models for the hagfish protease inhibitor were expanded to models for the twice as large human alpha 2-macroglobulin using symmetry operations, and the resulting alpha 2-macroglobulin models were found to agree with those emerged from earlier studies involving electron microscopy and X-ray scattering methods.
authors.affiliation: Department of Chemistry, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: R
authors.initials: R
authors.lastname: Osterberg
authors.forename: B
authors.initials: B
authors.lastname: Malmensten
authors.forename: A
authors.initials: A
authors.lastname: Ikai
mesh_terms: D000818:Animals; D006196:Hagfishes; D006801:Humans; D046911:Macromolecular Substances; D008961:Models, Structural; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000097:blood / Q000737:chemistry*; D011487:Protein Conformation; D014961:X-Ray Diffraction / Q000379:methods; D000511:alpha-Macroglobulins / Q000737:chemistry*
chemical_list: D046911:Macromolecular Substances; D011480:Protease Inhibitors; D000511:alpha-Macroglobulins