Connective tissue nevi collagens. Study with picrosirius red and polarizing microscopy.
Biopsy specimens of five connective tissue nevi were examined under crossed polars after staining with Picrosirius red. One biopsy specimen was from a solitary nevus, another from a Shagreen patch. The other three specimens were of erupted nevi. In all cases, thick (as well as thin) collagen fibers appeared green to yellow. In contrast, thick fibers of normal human dermis appeared orange to red. The findings indicate that the collagen of collagenous connective tissue nevi is less well packed than normal collagen. Examination of the polarization colors of Picrosirius red-stained sections is a useful procedure for diagnosing collagenous connective tissue nevi.
Trau
H
H
Department of Dermatology, Chaim Sheba Medical Center, Tel Aviv University, Israel.
Dayan
D
D
Hirschberg
A
A
Hiss
Y
Y
Bubis
J J
JJ
Wolman
M
M
D000328:Adult; D001391:Azo Compounds; D002675:Child, Preschool; D003094:Collagen / Q000032:analysis*; D003238:Connective Tissue / Q000737:chemistry*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008859:Microscopy, Polarization; D009506:Nevus / Q000737:chemistry*; D010851:Picrates; D012878:Skin Neoplasms / Q000737:chemistry*; D013194:Staining and Labeling
D001391:Azo Compounds; D010851:Picrates; C009798:C.I. direct red 80; D003094:Collagen