Sequenase should be used instead of the Klenow fragment for the synthesis of oligonucleotides labeled to a high specific activity.
Rigaud
G
G
Institut J. Monod du CNRS, University Paris 7, France.
Grange
T
T
Pictet
R
R
D004256:DNA Polymerase I / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000096:biosynthesis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism
D004277:DNA, Single-Stranded; D009841:Oligonucleotides; C034970:bacteriophage T7 induced DNA polymerase; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase