title: Regeneration of adsorbed and covalently immobilized antibodies on solid phases for immunoassay.
abstract: A technique for the reproducible re-use of antibody-coated solid phases for immunoassays is described. The method based on the dissociation of the antigen-antibody complexes. Two different procedures, using glycine buffer (pH 2.3) or ethanolamine, were developed. More than ten immunoassay cycles can be realized with the same antibody-coated microtitre plates. These procedures were tested with competitive and sandwich immunoassays, monoclonal and polyclonal antibodies, and a commercial immunoassay kit.
authors.affiliation: Forschungsinstitut für Medizinische Diagnostik, Dresden, DDR.
authors.forename: D
authors.initials: D
authors.lastname: Ilchmann
authors.forename: D
authors.initials: D
authors.lastname: Helbig
authors.forename: H
authors.initials: H
authors.lastname: Göhler
authors.forename: M
authors.initials: M
authors.lastname: Stopsack
authors.forename: H J
authors.initials: HJ
authors.lastname: Thiele
authors.forename: W
authors.initials: W
authors.lastname: Hubl
mesh_terms: D000327:Adsorption; D000469:Alkaline Phosphatase / Q000032:analysis* / Q000276:immunology; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007202:Indicators and Reagents; D011374:Progesterone / Q000032:analysis* / Q000276:immunology; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007202:Indicators and Reagents; D000509:alpha-Fetoproteins; D011374:Progesterone; D000469:Alkaline Phosphatase