Production and characterization of new monoclonal antibodies to human osteoclasts.
Two monoclonal antibodies raised against human osteoclastoma were found to show antiosteoclastic activity on frozen sections of tumor. Immunoreactivity was localized on the membrane surface. These antibodies exhibited no activity against tissue macrophages and human visceral tissue except kidney, where they stained tubules but not glomeruli. In addition, no activity was observed against rabbit or rat osteoclasts, suggesting that they might react with unique epitopes on human osteoclasts.
Nelson
P N
PN
Department of Histopathology, St. George's Hospital Medical School, London, U.K.
Pringle
J A
JA
Chambers
T J
TJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001859:Bone Neoplasms / Q000276:immunology*; D005629:Frozen Sections; D005870:Giant Cell Tumors / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D010010:Osteoclasts / Q000276:immunology*; D010641:Phenotype; D013045:Species Specificity; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal