title: Hypoxia inhibits calcium influx in rabbit basilar and carotid arteries.
abstract: We examined the hypothesis that hypoxia inhibits Ca2+ influx in isolated rabbit common carotid, internal carotid, and basilar arteries. In arteries mounted for measurement of isometric tension and exposed to 122 mM K+ in Ca(2+)-free Krebs, cumulative addition of Ca2+ produced Ca(2+)-force relations that were right-shifted by hypoxia (PO2 approximately 15 Torr) with no decrease in maximum force attained. In arteries precontracted with 122 mM K+, exposure to hypoxia produced relaxations whose rates and magnitudes were enhanced by reductions in bath Ca2+ from 8.0 to 0.8 mM. Using an ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid method for 3-min 45Ca influx measurements, modified for use in rabbit basilar and carotid arteries, we found that resting levels of Ca2+ influx (mumol.min-1.kg dry wt-1) were significantly higher in basilar (67 +/- 1, n = 10) than in internal carotid (27 +/- 1, n = 12) or common carotid (33 +/- 1, n = 12) arteries. K+ stimulation increased Ca2+ influx more than two-fold compared with control in all three artery types, and hypoxia inhibited this increase by 74% in basilar, 49% in internal carotid, and 33% in common carotid arteries. Exposure to 10 microM serotonin and 100 microM uridine 5'-triphosphate (UTP) also increased Ca2+ influx, but these increases were less than observed during K+ contractions and averaged 10 (basilar), 31 (internal carotid), and 82% (common carotid) above control. Hypoxia completely inhibited serotonin- and/or UTP-induced increases in Ca2+ influx in basilar and internal carotid segments and inhibited 47% of this increase in the common carotid segments.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology, Loma Linda University School of Medicine, California 92350.
authors.forename: W J
authors.initials: WJ
authors.lastname: Pearce
authors.forename: S
authors.initials: S
authors.lastname: Ashwal
authors.forename: D M
authors.initials: DM
authors.lastname: Long
authors.forename: J
authors.initials: J
authors.lastname: Cuevas
mesh_terms: D000818:Animals; D001488:Basilar Artery / Q000378:metabolism* / Q000503:physiopathology; D002118:Calcium / Q000037:antagonists & inhibitors* / Q000493:pharmacokinetics; D002339:Carotid Arteries / Q000378:metabolism* / Q000503:physiopathology; D005110:Extracellular Space / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism* / Q000503:physiopathology; D066298:In Vitro Techniques; D009994:Osmolar Concentration; D011817:Rabbits; D014664:Vasodilation
chemical_list: D002118:Calcium