Low-conductance chloride channel from crayfish skeletal muscle incorporated into planar lipid bilayers.
Low-conductance chloride channel from skeletal muscle SR vesicles of the crayfish Astacus fluviatilis was incorporated into planar lipid bilayers and its basic characteristics were investigated. The channel has a relatively low unitary conductance of 26 pS in symmetrical 160 mmol/l choline-chloride. The dependence of the channel conductance on Cl- concentration shows saturating behavior with a maximum conductance of 37 pS and an ionic activity for half-maximum conductance Km = 75 mmol/l. The channel exhibits a complex kinetics with several modes of activity. Open state probability slightly decreases with the increasing absolute value of voltage. The channel activity does not appear to be dependent on the presence of Ca2+ ions. The channel is effectively inhibited by DIDS, a stilbene derivative. The permeability properties of the channel are similar to the specific behavior of the "double-barrelled" channel from Torpedo electroplax described by Miller and White (1984).
Proks
P
P
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Hurnák
O
O
Zachar
J
J
D000818:Animals; D003400:Astacoidea / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002412:Cations / Q000378:metabolism; D002498:Centrifugation; D002712:Chlorides / Q000378:metabolism*; D002794:Choline / Q000494:pharmacology; D004553:Electric Conductivity; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000378:metabolism*; D008051:Lipid Bilayers / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000737:chemistry*
D002412:Cations; D002712:Chlorides; D007473:Ion Channels; D008051:Lipid Bilayers; D002794:Choline; D002118:Calcium