title: The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family.
abstract: A full-length cDNA clone (MB3) and three partial clones (MA1, MB1 and MB2) which encode myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1) were isolated from a Sinapis alba (white mustard) cDNA library. Nucleotide sequence analysis of these clones revealed that they are encoded by a gene family. Southern blot analysis with gene-specific probes showed that the gene family consists of a least two subfamilies (MA and MB) each with several members both in S. alba and in Brassica napus (oilseed rape). In Arabidopsis thaliana (wall cress) only three myrosinase genes seem to be present. Northern blot analysis indicated that all the myrosinase mRNA species have the same size, approximately 1.95 kb.
authors.affiliation: Department of Cell Research, Swedish University of Agricultural Sciences, Uppsala Genetic Center.
authors.forename: J P
authors.initials: JP
authors.lastname: Xue
authors.forename: M
authors.initials: M
authors.lastname: Lenman
authors.forename: A
authors.initials: A
authors.lastname: Falk
authors.forename: L
authors.initials: L
authors.lastname: Rask
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D001937:Brassica / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009149:Mustard Plant / Q000201:enzymology / Q000235:genetics; D010449:Peptide Mapping; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D006026:Glycoside Hydrolases; C022739:thioglucosidase