title: Expression site-associated genes transcribed independently of variant surface glycoprotein genes in Trypanosoma brucei.
abstract: Expression site-associated genes (ESAGs) of Trypanosoma brucei are found upstream of variant surface glycoprotein (VSG) genes in bloodstream expression sites. There are at least 6 different ESAGs in each of these expression sites, and each ESAG is repetitive in the genome. ESAGs are believed to reside only in VSG expression sites and to be co-transcribed with the VSG gene from a common alpha-amanitin-insensitive promoter. Our results show that this is not always true. The transcriptionally active 1.22 metacyclic expression site contains no ESAGs, but ESAGs are highly transcribed in these cells. The level of transcription indicates that more than one copy of each of these genes is active. Furthermore, some of these genes are transcribed, to produce steady state RNA, in procyclic culture cells which do not express the VSG gene: there is differential expression of ESAGs between the bloodstream and procyclic phases of the trypanosome life cycle. Thus ESAGs can be transcribed outwith an active VSG gene expression site and in the absence of expression of the VSG.
authors.affiliation: Institute of Genetics, University of Glasgow, Scotland, U.K.
authors.forename: S V
authors.initials: SV
authors.lastname: Graham
authors.forename: J D
authors.initials: JD
authors.lastname: Barry
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000469:parasitology; D005786:Gene Expression Regulation; D005796:Genes; D051379:Mice; D011401:Promoter Regions, Genetic; D012313:RNA / Q000096:biosynthesis; D015183:Restriction Mapping; D012469:Salivary Glands / Q000469:parasitology; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000254:growth & development; D014370:Tsetse Flies / Q000469:parasitology; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D014643:Variant Surface Glycoproteins, Trypanosoma; D012313:RNA