Identification of myenteric neurons which project to the mucosa of the guinea-pig small intestine.
Myenteric neurons which innervate the mucosa of the guinea-pig ileum were characterized by combining retrograde transport of DiI in vitro with immunohistochemistry. Of DiI-labelled myenteric neurons, 43% were immunoreactive for calbindin and substance P, 25% were immunoreactive for calbindin alone, and 18% were immunoreactive for substance P alone. These 3 classes of neurons had Dogiel Type II morphology and are probably sensory neurons. Two classes of probable secretomotor neurons were characterized by immunoreactivity for neuropeptide Y (4%) and vasoactive intestinal peptide (2%). These 5 classes of myenteric neurons represent over 90% of the retrogradely labelled myenteric neurons that project to the mucosa.
Song
Z M
ZM
Department of Human Physiology, Centre for Neuroscience, Flinders University of South Australia, Adelaide.
Brookes
S J
SJ
Costa
M
M
D000818:Animals; D064026:Calbindins; D002232:Carbocyanines; D006168:Guinea Pigs; D007082:Ileum / Q000294:innervation / Q000648:ultrastructure; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000294:innervation* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D009197:Myenteric Plexus / Q000166:cytology*; D009434:Neural Pathways / Q000166:cytology; D009474:Neurons / Q000648:ultrastructure*; D009478:Neuropeptide Y / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D064026:Calbindins; D002232:Carbocyanines; D009478:Neuropeptide Y; D064030:S100 Calcium Binding Protein G; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; C024286:3,3'-dioctadecylindocarbocyanine