title: Cytophotometric assessment of Feulgen-DNA and coomassie-total cell protein in adrenocortical fasciculata cells of noise-exposed Wistar rats.
abstract: The present investigation was undertaken to examine possible noise-induced alterations in adrenal fasciculata cell (AFC) metabolism, and also to determine if the magnitude of these changes differs in male versus female rats. Wistar rats approximately 3 months old were exposed to intense noise for 60 min (100 dB, re 2 x 10(-5) N(m2)-1, 350-20,000 Hz); control rats were housed under identical conditions, at an ambient noise level of 40-60 dB. Adrenal fasciculata cells (AFC) from each animal were examined for noise-induced alterations in Feulgen-DNA reactivity (as an indicator of chromatin template activity) and Coomassie-total cell protein levels using scanning-integrating cytophotometry. The results provide evidence that intense noise elicited a marked AFC metabolic enhancement in both male and female rats; the degree of this enhancement was more pronounced in males. This disparity may be due to pre-existing differences in male versus female AFC enzymatic capability and subsequent responsiveness to noise-induced activation.
authors.affiliation: Department of Biology, Pennsylvania State University, University Park 16802.
authors.forename: D U
authors.initials: DU
authors.lastname: Ballough
authors.forename: G P
authors.initials: GP
authors.lastname: Ballough
authors.forename: J A
authors.initials: JA
authors.lastname: Strauss
authors.forename: M J
authors.initials: MJ
authors.lastname: Durkot
authors.forename: A
authors.initials: A
authors.lastname: Anthony
mesh_terms: D000818:Animals; D002843:Chromatin / Q000378:metabolism; D003592:Cytophotometry / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D005260:Female; D008297:Male; D009622:Noise / Q000009:adverse effects*; D010503:Periodic Acid-Schiff Reaction; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012394:Rosaniline Dyes; D012727:Sex Characteristics; D013194:Staining and Labeling; D014158:Transcription, Genetic; D015383:Zona Fasciculata / Q000378:metabolism*
chemical_list: D002843:Chromatin; D011506:Proteins; D012394:Rosaniline Dyes; C048139:Coomassie blue; D004247:DNA