Experimental cutaneous protothecosis in mice: epithelioid cell granuloma formation.
Prototheca wickerhamii, isolated from skin biopsy specimens of a patient with cutaneous protothecosis, were cultured in Sabouraud's medium and inoculated in the skin of 8 ICR albino mice and 3 BALB/c mice. Only in 3 ICR albino mice and 3 BALB/c mice were organisms found in skin tissue, as confirmed by identification of organisms by staining with periodic acid-Schiff (PAS) stain and culture. Histologic findings from affected nodular skin lesions indicated epithelioid cell granuloma and histiocytic cell infiltrates in the dermis and subcutaneous tissue.
Horiuchi
Y
Y
Division of Dermatology, Kashima Rosai Hospital, Ibaraki, Japan.
Tomizawa
N
N
Higuchi
I
I
Nagasaka
K
K
D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004848:Epithelium / Q000473:pathology; D005260:Female; D006099:Granuloma / Q000382:microbiology* / Q000473:pathology; D007239:Infections; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D011525:Prototheca; D012867:Skin / Q000473:pathology; D012874:Skin Diseases, Infectious / Q000382:microbiology* / Q000473:pathology; D013194:Staining and Labeling