The release of neuronal 5-HT from the intestine of a teleost fish, Platycephalus bassensis.
The superfused, isolated intestine of a teleost fish which lacks enterochromaffin cells spontaneously released 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA), presumably from enteric neurons. The release of 5-HT, but not 5-HIAA, increased on transmural electrical stimulation. Addition of tetrodotoxin or omission of Ca2+ from the superfusate prevented the increase in 5-HT release on electrical stimulation. Fluoxetine added to the superfusate increased the amount of 5-HT released spontaneously but also prevented the increase in 5-HT release on stimulation. Pretreatment of fish with reserpine markedly reduced tissue levels of 5-HT and 5-HIAA and led to an almost complete loss of the spontaneous release of 5-HT and an elimination of the stimulated release of 5-HT.
Anderson
C R
CR
Department of Zoology, University of Melbourne, Australia.
Campbell
G
G
O'Shea
F
F
Payne
M
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004558:Electric Stimulation; D005399:Fishes; D005473:Fluoxetine / Q000494:pharmacology; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D012110:Reserpine / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D005473:Fluoxetine; D012701:Serotonin; D013779:Tetrodotoxin; D006897:Hydroxyindoleacetic Acid; D012110:Reserpine