Effect of pentosan polysulphate, standard heparin and related compounds on protein kinase C activity.
Ca2+/phospholipid-dependent protein kinase (PKC) was inhibited by sulphated polysaccharides. Pentosan polysulphate (PPS) and heparin were 8-10-times more potent than dextran sulphate or heparan sulphate. Steady-state studies revealed that PPS was a competitive inhibitor with respect to ATP with an apparent Ki value of 0.32 micrograms/ml and a non-competitive inhibitor with respect to histones. In contrast, the inhibition of PKC by heparin was competitive with substrate and non-competitive with respect to ATP. The interaction of sulphated polysaccharides with the catalytic domain of PKC was further demonstrated by the absence of effect on [3H]phorbol 12,13-dibutyrate binding to the regulatory domain of PKC. Furthermore, PPS and heparin inhibited equally cAMP-dependent protein kinase and tyrosine protein kinase. Structure-function relationships indicated that the Inhibition of protein kinases by PPS and heparin fractions was highly dependent on molecular weight. Additionally, PKC-affinity chromatography revealed that a high-molecular-weight heparin fraction with strong anti-PKC activity was eluted. We set out to demonstrate that heparin and PPS, which are potent antiproliferative agents on vascular smooth muscle cells (SMC), alter intracellular PKC activity (both membrane and cytosolic). Therefore, it is suggested that the mechanism by which sulphated polysaccharides inhibit SMC growth may be by direct inhibition of PKC in SMC.
Herbert
J M
JM
Unité de Biochimie Exploratoire, Sanofi Recherche, Toulouse, France.
Maffrand
J P
JP
D000818:Animals; D001921:Brain / Q000201:enzymology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D006493:Heparin / Q000031:analogs & derivatives / Q000494:pharmacology*; D007700:Kinetics; D008223:Lymphoma; D008823:Micelles; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D010426:Pentosan Sulfuric Polyester / Q000031:analogs & derivatives / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011817:Rabbits; D051381:Rats
D008823:Micelles; D011134:Polysaccharides; D010426:Pentosan Sulfuric Polyester; D006493:Heparin; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C