A monoclonal antibody sandwich immunoassay for serum amyloid A (SAA) protein.
An antibody sandwich immunoassay using two purified rat monoclonal antibodies to human serum amyloid A was developed and used to measure serum amyloid A in human serum. The assay was specific, sensitive, reproducible, and reliable and does not require denaturation of the specimen prior to assay. Serum amyloid A purified by hydrophobic interaction chromatography of acute phase human serum afforded a reliable standard for the assay. A significant (r = 0.69) correlation for SAA and C reactive protein values was found for 180 patient samples analyzed.
McDonald
T L
TL
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-6495.
Weber
A
A
Smith
J W
JW
D000911:Antibodies, Monoclonal / Q000276:immunology; D002097:C-Reactive Protein / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006801:Humans; D000685:Serum Amyloid A Protein / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D000685:Serum Amyloid A Protein; D002097:C-Reactive Protein