MHC class I-restricted presentation of exogenous antigen by thymic antigen-presenting cells in vitro and in vivo.
We have used a T-T hybridoma, RF33.70, to detect the MHC class I-restricted presentation of exogenous native OVA by thymic APC in vitro. Presentation of OVA with class I molecules by thymic APC requires intracellular processing. Phenotypic analyses indicated that low bouyant density, MHC class II+, FcR+ cells are capable of using this presentation pathway. In order to determine whether thymic APC have this function in vivo, thymic APC were isolated from mice after i.v. injection of native OVA. We find that OVA is presented in association with MHC class I, but not class II, molecules in the thymus. This is in contrast to splenic APC, which present exogenous OVA with both class I and class II molecules under these conditions. Our findings have implications for the repertoire of self-peptides that might be presented by thymic APC to developing T lymphocytes.
Grant
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EP
Dana-Farber Cancer Institute, Division of Lymphocyte Biology, Boston, MA 02115.
Rock
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KL
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001386:Azides / Q000494:pharmacology; D002469:Cell Separation; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D008211:Lymphocyte Cooperation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D011961:Receptors, Fc / Q000378:metabolism; D012276:Ricin / Q000633:toxicity; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*
D001386:Azides; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D011961:Receptors, Fc; D010047:Ovalbumin; D012276:Ricin