Interaction of fatty acid binding protein with microsomes: removal of palmitic acid and retinyl esters.
[14C] palmitic acid or [3H] retinyl esters incorporated in microsomal membranes were removed by a cytosolic fraction enriched in fatty acid binding protein. When mouse liver cytosol was fractionated by 70% ammonium sulphate, a precipitate and a soluble fraction were obtained. The soluble fraction containing the fatty acid binding protein was able to remove from microsomal membranes, [14C] palmitic acid or [3H] retinyl esters, whereas the precipitate fraction had no removal capacity. Retinoid analysis indicated that 70% ammonium sulphate soluble fraction was enriched in endogenous retinyl esters with regard to cytosol or 70% ammonium sulphate precipitate fraction.
Zanetti
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R
Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina.
Catalá
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D000645:Ammonium Sulfate; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D003600:Cytosol / Q000737:chemistry; D004952:Esters / Q000302:isolation & purification / Q000378:metabolism; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D051379:Mice; D008862:Microsomes, Liver / Q000737:chemistry*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding; D012995:Solubility; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism
D002352:Carrier Proteins; D004952:Esters; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010169:Palmitic Acids; D014801:Vitamin A; D019308:Palmitic Acid; D000645:Ammonium Sulfate