[Epitope specificity of hemagglutinating monoclonal anti-B antibodies].
Fine epitope specificity of ten monoclonal antibodies (MA) agglutinating red blood cells B was studied. Three methods were used: 1) inhibition of MA binding to natural antigen by synthetic oligosaccharides (OS) and their polyacrylamide conjugates, 2) direct MA binding to a series of synthetic OS-polyacrylamide conjugates differing in carbohydrate epitope density, 3) direct MA binding to the affinity sorbents. It is shown that all antibodies studied prefer trisaccharide B determinant Gal alpha 1-3(Fuc alpha 1-2) Gal independently of their ability to discriminate serological subgroups of B erythrocytes (B, Bweak, B3). The correlation of the MAs epitope specificity with their ability to agglutinate red blood cells B subgroups is discussed. Of an interest is that MAs which are able to agglutinate any B subgroups also bing the synthetic tetrasaccharide Gal alpha 1-3(Fuc alpha 1-2)Gal beta 1-3GalNAc, a B type 3 determinant.
Galanina
O E
OE
Deriugina
E I
EI
Olovnikova
N I
NI
Nosyrev
A E
AE
Lapenkov
M I
MI
Chekneva
N B
NB
Zemlianukhina
T V
TV
Korchagina
E Iu
EIu
Bovin
N V
NV
D000017:ABO Blood-Group System / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006384:Hemagglutination; D006801:Humans; D008567:Membranes, Artificial; D009844:Oligosaccharides; D013379:Substrate Specificity
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D008567:Membranes, Artificial; D009844:Oligosaccharides
Epitopnaia spetsifichnost' gemaggliutiniruiushchikh monoklonal'nykh anti-B-antitel.