Activation of guinea pig alveolar macrophages by endothelin-1.
Endothelin-1 (ET-1) induced a concentration- and time-dependent increase in arachidonic acid release from guinea pig alveolar macrophages, a maximum being reached at 1 nM ET-1. Regarding the time-course study, maximal release of arachidonic acid was observed after 30 s of incubation with ET-1 and then followed by a marked decrease, suggesting a reuptake of free arachidonic acid. ET-1 (0.1-10 nM) induced a concentration- and time-dependent release of TXB2, the stable metabolite of TXA2, evaluated by an ELISA technique. Maximum release was also obtained with 1 nM ET-1 but only after a 1-min incubation period. By contrast, ET-1 (1 pM-1 microM) did not stimulate the release of superoxide anion at any time point of the kinetic study. These results suggests that ET-1 may activate guinea pig alveolar macrophages, probably through a mechanism involving the arachidonic acid pathway.
Millul
V
V
URA CNRS 1283, CHU St Antoine, Paris, France.
Lagente
V
V
Gillardeaux
O
O
Boichot
E
E
Dugas
B
B
Mencia-Huerta
J M
JM
Béréziat
G
G
Braquet
P
P
Masliah
J
J
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D016232:Endothelins / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects*; D016676:Macrophages, Alveolar / Q000187:drug effects*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D013929:Thromboxane B2 / Q000378:metabolism
D016232:Endothelins; D016718:Arachidonic Acid; D013929:Thromboxane B2; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008165:Luminol