Regulatory volume decrease in small intestinal crypts is inhibited by K+ and Cl- channel blockers.
Total crypt volume has been estimated by analysis of photographic images of intact viable crypts isolated from guinea-pig small intestine. Exposing these crypts to a hypotonic medium, led to transient swelling followed by regulatory volume decrease (RVD) in 12-20 min. RVD was blocked by inhibitors of K+ and Cl- conductance, suggesting that it occurs by activation of K+ and Cl- permeability pathways and loss of these ions.
O'Brien
J A
JA
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
Walters
R J
RJ
Sepúlveda
F V
FV
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology*; D001464:Barium / Q000494:pharmacology*; D017609:Barium Compounds; D018118:Chloride Channels; D002712:Chlorides / Q000378:metabolism; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000502:physiology*; D007421:Intestine, Small / Q000502:physiology; D007473:Ion Channels / Q000502:physiology*; D008565:Membrane Proteins / Q000187:drug effects / Q000502:physiology*; D008871:Microvilli / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D013937:Thymidine Kinase / Q000378:metabolism
D000873:Anthracenes; D017609:Barium Compounds; D018118:Chloride Channels; D002712:Chlorides; D007473:Ion Channels; D008565:Membrane Proteins; D015221:Potassium Channels; C024986:barium chloride; D001464:Barium; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C010030:9-anthroic acid; D013937:Thymidine Kinase; D000469:Alkaline Phosphatase; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D011188:Potassium