[A monoclonal antibody study of the receptor area of the Venezuelan encephalitis virus].
The receptor region for virus-cell interaction in Venezuelan equine encephalomyelitis (VEE) and Eastern equine encephalomyelitis (EEE) viruses was studied using a panel of 17 monoclonal antibodies (MCA). They were able to block agglutination of goose erythrocytes. The dominant role of glycoprotein E2 in the formation of viral receptor for EEE and VEE viruses was demonstrated. Competitive radioimmunoassay identified three antigenic sites in this region. These sites were also responsible for virus neutralization. MCAs to these sites protected outbred mice against lethal infection. The presence of a highly conservative region in VEE (site E2-3) and EEE (site E2a) which produced cross-reacting antibodies blocking hemagglutination of Western equine encephalomyelitis, Semliki Forest, Sindbis, Getah, Aura, Chikungunya, and Pixuna viruses was established. A hypothesis is suggested concerning the existence of similar regions for the entire alphavirus genus, and the role of this region in virus-cell interaction.
Razumov
I A
IA
Pereboev
A V
AV
Protopopova
E V
EV
Khusainova
A D
AD
Agapov
E V
EV
Mel'nikova
E E
EE
Gaĭdamovich
S Ia
SIa
Loktev
V B
VB
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004663:Encephalitis Virus, Eastern Equine / Q000276:immunology; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D009500:Neutralization Tests; D015531:Radioimmunoprecipitation Assay; D051381:Rats; D011991:Receptors, Virus / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D011991:Receptors, Virus
Izuchenie retseptornoĭ oblasti virusa venesuél'skogo éntsefalita loshadeĭ pri pomoshchi monoklonal'nykh antitel.