x121: a localized maternal transcript in Xenopus laevis.
We describe the cloning and characterization of a partial cDNA, x121, that represents an RNA, which is localized in the animal hemisphere of Xenopus oocytes. This RNA is also detected in an animal to vegetal gradient during early cleavage stages. The x121 RNA titer decreases from fertilization through the gastrula stage, after which it is not detectable on northern blots. The amino acid composition of the x121 conceptual protein derived from cDNA sequencing reveals a large number of acidic residues similar in distribution to proteins that function as transcriptional activators.
Kloc
M
M
Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Reddy
B A
BA
Miller
M
M
Eastman
E
E
Etkin
L D
LD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA; D004527:Egg Proteins / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D015723:Gene Library; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D029867:Xenopus Proteins; D014982:Xenopus laevis
D004527:Egg Proteins; D029867:Xenopus Proteins; C069799:x121 protein, Xenopus laevis; D012313:RNA; D004247:DNA