Bacterial endotoxin-induced expression of metallothionein genes in rat brain, as revealed by in situ hybridization.
In order to clarify acute-phase response in brain, we investigated induction of metallothionein (MT) genes by administrating an endotoxin (lipopolysaccharide) in rat intraperitoneum. We performed in situ hybridization on the serial brain sections to identify the cells expressing the MT genes in acute-phase. After endotoxin administration, transcripts of MT genes were detected in the arachnoideal, ependymal cells and glial cells around the Purkinje cells of the cerebellum, while no significant induction of the MT genes by zinc ion was observed in brain. These results suggest that the acute-phase response occurs specifically in at least these 3 non-neuronal cells.
Itano
Y
Y
Department of Anesthesiology and Resuscitology, Okayama University Medical School, Japan.
Noji
S
S
Koyama
E
E
Taniguchi
S
S
Taga
N
N
Takahashi
T
T
Ono
K
K
Kosaka
F
F
D000209:Acute-Phase Proteins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D004731:Endotoxins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D015032:Zinc / Q000494:pharmacology
D000209:Acute-Phase Proteins; D004731:Endotoxins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D008668:Metallothionein; D015032:Zinc