Fasciola hepatica: immunoprecipitation analysis of biosynthetically labelled antigens using sera from infected sheep.
The antigenicity of the biosynthetically labelled somatic and excretory/secretory proteins of adult Fasciola hepatica was investigated over 20 weeks of an infection with F. hepatica in sheep. The antibody response was initially detected by ELISA 2 weeks after infection, and was sustained at this level for the remainder of the infection. Immunoprecipitation analysis indicated that a large number of proteins were recognized by the sheep, with several dominant antigens occurring in the 29-31 kilodalton molecular weight range. No differences were found between the antigens recognized by sheep in the early or late stages of infection suggesting that there are similarities between the antigens of the immature and mature forms of the parasite.
Sexton
J L
JL
Veterinary Research Institute, Department of Agriculture and Rural Affairs, Attwood, Victoria, Australia.
Milner
A R
AR
Campbell
N J
NJ
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology / Q000662:veterinary*; D011233:Precipitin Tests / Q000379:methods; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000939:Epitopes