Synthetic peptides in HIV antibody screening and typing.
We have defined continuous native epitopes of HIV proteins by using a systematic epitope-scanning technology. We have demonstrated that there is a highly immunoreactive continuous native epitope region in the transmembrane protein gp41 of HIV-1 that is immunoreactive with all studied HIV-1 antibody-positive sera. The corresponding region in HIV-2 gp34 behaves similarly. There is a clear difference, however, between HIV type 1 and type 2 transmembrane proteins in the number of highly immunoreactive regions, when presented properly as synthetic antigens in solid-phase EIA, can provide tests unusually suitable for early and reliable diagnosis of HIV-1 and HIV-2 infections and for type-specific distinction of the two types of HIV infections.
Varnier
O E
OE
Laboratory of Human Retrovirology, School of Medicine, University of Genoa, Italy.
Närvänen
A
A
Korkolainen
M
M
Lillo
F
F
Kontio
S
S
Elm
J
J
Suni
J
J
Vaheri
A
A
Huhtala
M L
ML
D000939:Epitopes; D015483:HIV Antibodies / Q000032:analysis* / Q000145:classification; D015658:HIV Infections / Q000145:classification / Q000175:diagnosis; D006801:Humans; D010455:Peptides / Q000138:chemical synthesis* / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
D000939:Epitopes; D015483:HIV Antibodies; D010455:Peptides; D014764:Viral Proteins