title: Purification of neuropathy target esterase from avian brain after prelabelling with [3H]diisopropyl phosphorofluoridate.
abstract: Neuropathy target esterase from hen brains was radiolabelled at the active site with [3H]diisopropyl phosphorofluoridate. The labelled protein was purified by differential centrifugation and Nonidet P40 solubilization, detergent phase partitioning, anion exchange, and preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The volatilizable counts assay and analytical SDS-PAGE were used to monitor the protein. The 150-kDa subunit polypeptide appears as a single band on analytical SDS-PAGE.
authors.affiliation: MRC Toxicology Unit, Medical Research Council Laboratories, Surrey, England, U.K.
authors.forename: M E
authors.initials: ME
authors.lastname: Rüffer-Turner
authors.forename: D J
authors.initials: DJ
authors.lastname: Read
authors.forename: M K
authors.initials: MK
authors.lastname: Johnson
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification*; D002498:Centrifugation; D002645:Chickens; D002852:Chromatography, Ion Exchange; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D007531:Isoflurophate; D017830:Octoxynol; D011092:Polyethylene Glycols; D012995:Solubility; D014316:Tritium
chemical_list: D003902:Detergents; D014316:Tritium; D007531:Isoflurophate; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D002265:Carboxylic Ester Hydrolases; C021859:neurotoxic esterase