[A new method of isolation and a new form of transketolase from baker's yeast].
A new procedure for the isolation of homogeneous transketolase from baker's yeast based on the use of enzyme-specific antibodies immobilized on a insoluble matrix has been developed. The enzyme yield is 90% of its total content in the original yeast extract. The eluate from the immunocolumn was found to contain a previously unknown form of transketolase which represents an enzyme-RNA complex.
Tikhomirova
N K
NK
Kochetov
G A
GA
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D014174:Transketolase / Q000302:isolation & purification* / Q000378:metabolism; D015003:Yeasts / Q000201:enzymology*
D007527:Isoenzymes; D012313:RNA; D014174:Transketolase; D012259:Ribonuclease, Pancreatic
Novyĭ metod vydeleniia i novaia forma transketolazy iz pekarskikh drozhzheĭ.