title: Human immunodeficiency virus type 1 infection of endothelial cells in vitro.
abstract: In order to establish whether endothelial cells are involved in immunodeficiency virus type 1 (HIV-1) infection, we performed a virological study on endothelial cells isolated from human adipose tissue and infected with HIV-1 in vitro. Supernatants from cultures showed a reverse transcriptase activity starting one day after HIV inoculation. Viral rescue was significantly impaired in cycloheximide treated cells confirming a de novo synthesis of viral products.
authors.affiliation: Institute of Microbiology, University of Bologna, S. Orsola Hospital, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Re
authors.forename: G
authors.initials: G
authors.lastname: Furlini
authors.forename: G
authors.initials: G
authors.lastname: Cenacchi
authors.forename: P
authors.initials: P
authors.lastname: Preda
authors.forename: M
authors.initials: M
authors.lastname: La Placa
mesh_terms: D000273:Adipose Tissue; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004727:Endothelium / Q000166:cytology / Q000382:microbiology*; D015683:Gene Products, gag / Q000032:analysis; D015488:HIV Antigens / Q000032:analysis; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000201:enzymology / Q000276:immunology / Q000502:physiology*; D006801:Humans; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D014758:Viral Core Proteins / Q000032:analysis; D014779:Virus Replication
chemical_list: D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D014758:Viral Core Proteins; D003513:Cycloheximide; D012194:RNA-Directed DNA Polymerase