Expression of the neuronal noradrenaline transporter in Xenopus laevis oocytes.
RNA isolated from both rat phaeochromocytoma (PC12) cells and bovine adrenal medulla was size-selected and injected into oocytes of Xenopus laevis. Expression of the neuronal noradrenaline transporter was assayed 3 days after injection by measuring the nisoxetine-sensitive noradrenaline uptake. A 2 kilobase fraction of either total RNA derived from both tissues or of polyadenylated mRNA obtained from bovine adrenal medulla caused Na(+)-dependent transport of 3H-noradrenaline in the injected oocytes.
Coppeneur
D
D
Department of Pharmacology and Toxicology University Bonn, Germany.
Lingen
B
B
Sanders
G
G
Dabauvalle
M C
MC
Bönisch
H
H
D000310:Adrenal Gland Neoplasms / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D009638:Norepinephrine / Q000378:metabolism; D050484:Norepinephrine Plasma Membrane Transport Proteins; D009865:Oocytes / Q000378:metabolism; D010673:Pheochromocytoma / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D051381:Rats; D027981:Symporters; D014316:Tritium; D014407:Tumor Cells, Cultured; D014982:Xenopus laevis / Q000378:metabolism*
D002352:Carrier Proteins; D050484:Norepinephrine Plasma Membrane Transport Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C493226:Slc6a2 protein, rat; D027981:Symporters; D014316:Tritium; D012313:RNA; D009638:Norepinephrine