[Total protein and RNA biosynthesis in the liver cells of the long-tailed suslik Citellus undulatus in different functional states].
The synthesis of proteins and RNA have been studied by means of labeled precursors of 14C-amino acids and 14C-orotic acid in liver cells of hibernating, arousing and active ground squirrels (Citellus undulatus). The investigations conducted testify to the possibility of CHI-inhibited synthesis of proteins in liver cells of hibernating ground squirrels, which occurs, apparently, on preexisting polyribosomes. The low level of protein biosynthesis in this state is stipulated not only by the Arrhenius dependence, but by the dissociation of polyribosomes into monosomes as well. In the course of arousal, an intensive assembling of polyribosomes was detected even under the inhibition of RNA synthesis by actinomycin D, testifying to the presence of a pool of mRNA [correction of iRNA] in liver cells, which are necessary for the observed activation of translation. The intensity of protein synthesis on arousal is also stimulated by the activation of transcription.
Zagnoĭko
V I
VI
Gulevskiĭ
A K
AK
Shcheniavskiĭ
I I
II
Il'iasova
E I
EI
D000818:Animals; D002250:Carbon Radioisotopes; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003864:Depression, Chemical; D006605:Hibernation / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects; D012313:RNA / Q000096:biosynthesis* / Q000187:drug effects; D012589:Sciuridae / Q000502:physiology*; D012621:Seasons; D014851:Wakefulness / Q000187:drug effects / Q000502:physiology
D002250:Carbon Radioisotopes; D011506:Proteins; D003609:Dactinomycin; D012313:RNA; D003513:Cycloheximide
Biosintez summarnykh belkov i RNK v kletkakh pecheni dlinnokhvostykh suslikov Citellus undulatus, nakhodiashchikhsia v razlichnykh funktsional'nykh sostoianiiakh.