title: Postnatal induction and neural regulation of inward rectifiers in mouse skeletal muscle.
abstract: The whole-cell voltage-clamp technique was used to examine developmental changes of inward rectifier currents in fibres of the flexor digitorum brevis muscle acutely isolated from mice on postnatal day 0 (P0) to P36. Neither a steady-state component (Is-s) nor a slowly activated component (Irise) of inward rectifier currents were observed in fibres of P0 and P4 mice. Both Is-s and Irise became apparent between days P8 and P16. The specific amplitudes of Is-s and Irise measured at a test-pulse potential of -100 mV at 20 mM extracellular K+ [( K+]o) increased to their respective platcau values of -68 +/- 10 and -15 +/- 7 microA/cm2 at P20. In fibres denervated on day P4 the developmental increase of Is-s was suppressed, its specific amplitude at P20 being one-tenth of that in the corresponding normal fibres. Irise did not appear in P4-denervated fibres throughout the development. In muscle fibres denervated at P16 or P20, the specific amplitudes of Is-s and Irise decreased, reaching the levels of P4-denervated fibres in 2-4 days after denervation. We conclude that Is-s and Irise develop within 3 weeks after birth, and suggest that innervation plays a key role in their induction.
authors.affiliation: Research Centre for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Gonoi
authors.forename: S
authors.initials: S
authors.lastname: Hasegawa
mesh_terms: D000367:Age Factors; D000818:Animals; D004553:Electric Conductivity; D005260:Female; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials; D051379:Mice; D008813:Mice, Inbred ICR; D009121:Muscle Denervation; D024510:Muscle Development; D009132:Muscles / Q000294:innervation / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism
chemical_list: D007473:Ion Channels; D015221:Potassium Channels; D008274:Magnesium