title: The polymerase chain reaction and related techniques.
abstract: The polymerase chain reaction, and other methods for rapidly amplifying DNA or RNA are versatile tools that can be used in diverse applications that include HLA typing, analyzing the T-cell repertoire, constructing chimaeric antibodies and quantifying cytokine expression. The sensitivity of the polymerase chain reaction has allowed many cellular events to be investigated in vitro and in vivo. Many currently emerging adaptions of the basic technique are of interest to both the research and the clinical immunologist.
authors.affiliation: Victorian Institute of Animal Science, Attwood, Victoria, Australia.
authors.forename: A M
authors.initials: AM
authors.lastname: Lew
mesh_terms: D000818:Animals; D002678:Chimera / Q000276:immunology; D004247:DNA / Q000032:analysis; D006650:Histocompatibility Testing; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D012333:RNA, Messenger; D012313:RNA; D004247:DNA