Radiation inactivation of ion channels formed by gramicidin A. Protection by lipid double bonds and by alpha-tocopherol.
The conductance induced by the channel-forming peptide gramicidin A in lipid membranes is reduced by many orders of magnitude on exposure of the membrane and its aqueous environment to ionizing radiation. This results from an interaction of free radicals of water radiolysis with the tryptophan residues of gramicidin A. The sensitivity of the ion channels towards irradiation is strongly reduced in the presence of either vitamin E or of highly unsaturated lipids. An increase of the D37 dose up to a factor of 50 was found. The phenomena are interpreted via a reduction of the effective concentration of free radicals (such as OH.) in the membrane by reaction with unsaturated fatty acid residues or with vitamin E.
Barth
C
C
Fakultät für Biologie, Universität Konstanz, F.R.G.
Stark
G
G
D004553:Electric Conductivity; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005609:Free Radicals; D006096:Gramicidin / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects / Q000528:radiation effects*; D008051:Lipid Bilayers; D008055:Lipids / Q000737:chemistry / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
D005231:Fatty Acids, Unsaturated; D005609:Free Radicals; D007473:Ion Channels; D008051:Lipid Bilayers; D008055:Lipids; D006096:Gramicidin; D014810:Vitamin E