title: Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products.
abstract: The nonobese diabetic (NOD) mouse, in which major histocompatibility complex genes may be involved in the susceptibility to diabetes, has been developed as a model of autoimmune diabetes. The NOD mouse expresses I-A-encoded class II major histocompatibility complex antigens, which differ from those of other mouse haplotypes by the presence of a serine at position 57 of the A beta chain. Identifying islet autoantigens may help elucidate the role of class II antigens in the activation of autoreactive T cells and, thus, in the development of diabetes. We have detected autoantibodies directed against a 58-kDa islet cell antigen in NOD mice but not in other strains, including lupus-prone mice. Apart from insulin-secreting cells, the 58-kDa antigen was only found to be expressed by neuroblastoma cells and was identified as peripherin, an intermediate filament protein previously characterized in well-defined neuronal populations. This autoantigen cross-reacted with I-Anod class II antigens, suggesting that it may contribute to defective self-tolerance of islet beta cells in the NOD mouse.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, U 25/Centre National de la Recherche Scientifique A 122, Hôpital Necker, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Boitard
authors.forename: M C
authors.initials: MC
authors.lastname: Villa
authors.forename: C
authors.initials: C
authors.lastname: Becourt
authors.forename: H P
authors.initials: HP
authors.lastname: Gia
authors.forename: C
authors.initials: C
authors.lastname: Huc
authors.forename: P
authors.initials: P
authors.lastname: Sempe
authors.forename: M M
authors.initials: MM
authors.lastname: Portier
authors.forename: J F
authors.initials: JF
authors.lastname: Bach
mesh_terms: D000367:Age Factors; D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D003429:Cross Reactions; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007381:Intermediate Filament Proteins / Q000737:chemistry / Q000276:immunology*; D007515:Islets of Langerhans / Q000276:immunology*; D008562:Membrane Glycoproteins; D051379:Mice; D016688:Mice, Inbred NOD / Q000276:immunology*; D009419:Nerve Tissue Proteins; D064531:Peripherins
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D000949:Histocompatibility Antigens Class II; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D064531:Peripherins