title: Use of acetic orcein as a fast method to relax, fix, clear and stain cercariae for light microscopy.
abstract: Acetic orcein stain may be dropped onto the specimen before applying a cover slip. This single reagent reveals cercarial and metacercarial structures that would otherwise be made visible only by complicated and time consuming procedures.
authors.affiliation: Zoology Department, University of Cape Town, Rondebosch, Republic of South Africa.
authors.forename: S C
authors.initials: SC
authors.lastname: Webb
mesh_terms: D000818:Animals; D010078:Oxazines; D013194:Staining and Labeling; D014200:Trematoda / Q000033:anatomy & histology*
chemical_list: D010078:Oxazines; C002313:PAcein