Evaluation of three techniques to demonstrate retrograde transport of horseradish peroxidase.
Three methods of tracing neural connections by the use of horseradish peroxidase (HRP) were evaluated: (1) endoneurium injection, (2) injection followed by crushing at site of injection, and (3) nerve transection followed by capping of the proximal stump with a silicone cylinder containing HRP. The capping technique resulted in increased uptake and more uniform distribution of HRP in the nerves under study.
Orsini
J A
JA
University of Pennsylvania, School of Veterinary Medicine, New Bolton Center, Kennett Square 19348.
Pollock
R V
RV
D000818:Animals; D001370:Axonal Transport; D001693:Biological Transport, Active; D004285:Dogs; D006735:Horseradish Peroxidase / Q000008:administration & dosage / Q000493:pharmacokinetics*; D009498:Neurotoxins / Q000493:pharmacokinetics; D010525:Peripheral Nerves / Q000378:metabolism*
D009498:Neurotoxins; D006735:Horseradish Peroxidase