Ethanol-induced growth inhibition of erythroleukemia stem cells. Cell cycle effects.
Friend erythroleukemia stem cells are strongly growth inhibited by 60 mM ethanol. The expression of this inhibition requires several days to develop fully, and is not relieved by washing into new ethanol-containing medium even in the presence of excess folic acid. Removal of the fully inhibited cells from ethanol results in rapid growth recovery, with the onset of recovery occurring within a few hours. Cell cycle analysis reveals a G1 delay which is evident within a few hours after initial ethanol exposure. Bivariate RNA-DNA analysis indicates that this G1 delay results from restriction in late G1. It is unclear at present whether this delay can account for all the observed growth inhibition.
Cook
R T
RT
Department of Pathology, Veterans Affairs Medical Center, Iowa City, Iowa.
Keiner
J A
JA
D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000032:analysis; D015672:Erythroid Precursor Cells / Q000473:pathology*; D000431:Ethanol / Q000494:pharmacology*; D005622:Friend murine leukemia virus; D016193:G1 Phase / Q000187:drug effects; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D012313:RNA / Q000032:analysis; D014407:Tumor Cells, Cultured
D000431:Ethanol; D012313:RNA; D004247:DNA