Establishment of an enzyme-linked immuno-sorbent assay for urinary trypsin inhibitor by using a monoclonal antibody.
Monoclonal antibodies against inter-alpha-trypsin-inhibitor (ITI) were produced. One clone showing specificity for urinary trypsin inhibitor (UTI), a proteolytic fragment of ITI, which is excreted into urine, was selected for the establishment of an enzyme-linked immuno-sorbent assay (ELISA). The ELISA for the quantification of UTI was shown to work reproducibly in the range between 0.5 and 10 ng UTI/ml urine. Urines of several patients suffering from different lung diseases were screened for UTI using the established ELISA. Highest UTI levels were found in the urine of patients with lung empyema. A more moderate increase was observed in patients suffering from lung tuberculosis and from secondary and primary lung tumors.
Trefz
G
G
Thoraxklinik Heidelberg-Rohrbach, Heidelberg-Rohrbach.
Streit
B
B
Justus
C W
CW
Ebert
W
W
Kramer
M D
MD
D000510:Alpha-Globulins / Q000652:urine*; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000652:urine; D004653:Empyema / Q000175:diagnosis / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D015151:Immunoblotting; D019272:Leukocyte Elastase; D008171:Lung Diseases / Q000175:diagnosis / Q000652:urine*; D008175:Lung Neoplasms / Q000652:urine; D010196:Pancreatic Elastase / Q000652:urine; D014361:Trypsin Inhibitors / Q000652:urine*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000652:urine; D000515:alpha 1-Antitrypsin / Q000652:urine
D000510:Alpha-Globulins; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D006023:Glycoproteins; D014361:Trypsin Inhibitors; D000515:alpha 1-Antitrypsin; C037741:alpha 1-antitrypsin-leukocyte elastase complex; C011877:inter-alpha-inhibitor; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; C028665:urinastatin