The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis.
Mouse anti-Fas monoclonal antibody has a cytolytic activity on human cells that express the antigen. Complementary DNAs encoding the cell surface antigen Fas were isolated from a cDNA library of human T cell lymphoma KT-3 cells. The nucleotide sequence of the cDNAs revealed that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide (Mr 36,000) with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. Murine WR19L cells or L929 cells transformed with the human Fas antigen cDNA were killed by the anti-Fas antibody in the process known as apoptosis.
Itoh
N
N
Osaka Bioscience Institute, Japan.
Yonehara
S
S
Ishii
A
A
Yonehara
M
M
Mizushima
S
S
Sameshima
M
M
Hase
A
A
Seto
Y
Y
Nagata
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002470:Cell Survival; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007739:L Cells / Q000648:ultrastructure; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011487:Protein Conformation; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D019014:fas Receptor
D000954:Antigens, Surface; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019014:fas Receptor