The adenohypophysis of the Australian lungfish, Neoceratodus forsteri--an immunocytological study.
The cell types in the adenohypophysis of Neoceratodus resemble closely those already described for Lepidosiren and Protopterus. Four of these were immunocytochemically identified as prolactin cells, gonadotropes, corticotropes, and melanotropes. Antiserum to bullfrog growth hormone could not distinguish between prolactin cells and somatotropes. Anti-bullfrog prolactin, however, did selectively stain the prolactin cells, which allowed the identification of the somatotropes. The presumptive thyrotropes, as the only remaining cell type in the pars distalis, can then be tentatively identified by default. Likewise a PAS-positive cell type in the pars intermedia had no immunoreactivity to any of the antisera used. The functional significance of this cell remains to be demonstrated. One of the more unexpected findings was the presence of large numbers of cells immunoreactive to alpha-MSH in the proximal pars distalis. The implications of the presence of these cells in adult lungfish are discussed. The distribution of cell types within the pituitary of Neoceratodus showed more regionalization than is present in the other lungfish and corresponded more closely to that described for primitive actinopterygian fish. The general structure of the pituitary of Neoceratodus also resembled primitive actinopterygian fish more closely than it did amphibians, unlike the pituitaries of Lepidosiren and Protopterus. The evolutionary significance of this is also discussed.
Joss
J M
JM
School of Biological Science, Macquarie University, Sydney, New South Wales, Australia.
Beshaw
M
M
Williamson
S
S
Trimble
J
J
Dores
R M
RM
D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000818:Animals; D005260:Female; D005399:Fishes / Q000033:anatomy & histology*; D006062:Gonadotropins / Q000032:analysis; D013006:Growth Hormone / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D008297:Male; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis; D010503:Periodic Acid-Schiff Reaction; D010903:Pituitary Gland, Anterior / Q000166:cytology*; D011388:Prolactin / Q000032:analysis; D013194:Staining and Labeling
D006062:Gonadotropins; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D013006:Growth Hormone; D009074:Melanocyte-Stimulating Hormones