Heterocycles related to nucleotides. XI. An organomercurial column chromatography for thiouridine.
By the organomercurial column: chromagel or Sephadex coupled to p-aminophenylmercury chloride, 4- and 2-thiouridine were selectively separated from the mixtures with major nucleotides. Oligonucleotides containing 4-thiouridilic acid were also separated from the ribonuclease-T1 digests of unfractionated transfer ribonucleic acid of E. coli.
Sato
E
E
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Suzuki
A
A
Kanaoka
Y
Y
Kimura
K
K
D002850:Chromatography, Gel; D004926:Escherichia coli / Q000235:genetics; D015394:Molecular Structure; D009705:Nucleosides / Q000302:isolation & purification; D009711:Nucleotides / Q000302:isolation & purification; D009841:Oligonucleotides / Q000302:isolation & purification; D010663:Phenylmercury Compounds; D012329:RNA, Bacterial / Q000302:isolation & purification; D012343:RNA, Transfer / Q000302:isolation & purification; D013891:Thiouridine / Q000302:isolation & purification*
D009705:Nucleosides; D009711:Nucleotides; D009841:Oligonucleotides; D010663:Phenylmercury Compounds; D012329:RNA, Bacterial; D013891:Thiouridine; C067422:4-aminophenylmercury chloride; D012343:RNA, Transfer