[The use of a CHO cell culture in studying Vibrio cholerae grown under different conditions].
Studies of biological activity of cholera vibrios in cultures of chinese hamster ovary cells (CHO) have revealed their strong dependence on culture conditions. Elongation of CHO cells is caused only by choleragenic strains. Under stationary conditions of culture the vibrios were found to release haemolisin into the medium and had a cytotoxic effect. Most of cytotoxic supernatants exhibited a neuraminidase activity. Proteolytic activity was less dependent on the vibrio culture conditions. Strains with a high proteolytic activity caused rounding of the CHO cells.
Sal'nikova
O I
OI
Lobanov
V V
VV
Shimaniuk
N Ia
NIa
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001431:Bacteriological Techniques; D002460:Cell Line; D002478:Cells, Cultured / Q000382:microbiology; D002772:Cholera Toxin / Q000096:biosynthesis; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D019081:O Antigens; D010053:Ovary; D011135:Polysaccharides, Bacterial / Q000032:analysis; D014734:Vibrio cholerae / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity
D000942:Antigens, Bacterial; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D002772:Cholera Toxin
Ispol'zovanie kul'tury kletok CHO pri izuchenii kholernykh vibrionov, vyrashchennykh v razlichnykh usloviiakh.