N-ethylmaleimide and mercurials modulate inhibition of the mitochondrial inner membrane anion channel by H+, Mg2+ and cationic amphiphiles.
Previously it has been shown that the mitochondrial inner membrane anion channel is reversibly inhibited by matrix Mg2+, matrix H+ and cationic amphiphiles such as propranolol. Furthermore, the IC50 values for both Mg2+ and cationic amphiphiles are dependent on matrix pH. It is now shown that pretreatment of mitochondria with N-ethylmaleimide, mersalyl and p-chloromercuribenzenesulfonate increases the IC50 values of these inhibitors. The effect of the mercurials is most evident when cysteine or thioglycolate is added to the assay medium to reverse their previously reported inhibitory effect (Beavis, A.D. (1989) Eur. J. Biochem. 185, 511-519). Although the IC50 values for Mg2+ and propranolol are shifted they remain pH dependent. Mersalyl is shown to inhibit transport even in N-ethylmaleimide-treated mitochondria indicating that N-ethylmaleimide does not react at the inhibitory mercurial site. However, the effects of N-ethylmaleimide and mersalyl on the IC50 for H+ are not additive which suggests that mercurials and N-ethylmaleimide react at the same 'regulatory' site. It is suggested that modification of this latter site exerts an effect on the binding of Mg2+, H+ and propranolol by inducing a conformational change. It is also suggested that a physiological regulator may exist which has a similar effect in vivo.
Beavis
A D
AD
Department of Pharmacology, Medical College of Ohio, Toledo 43699-0008.
D000818:Animals; D000838:Anions / Q000378:metabolism; D001665:Binding Sites; D001692:Biological Transport; D002712:Chlorides / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007473:Ion Channels / Q000187:drug effects* / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008634:Mersalyl / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000648:ultrastructure; D009941:Organomercury Compounds / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000838:Anions; D002712:Chlorides; D007473:Ion Channels; D009941:Organomercury Compounds; D013438:Sulfhydryl Compounds; D008634:Mersalyl; D011433:Propranolol; D008274:Magnesium; D005033:Ethylmaleimide