Cholinergic nigrotectal projections in the rat.
In order to verify a possible target site of cholinergic neurons in the substantia nigra of the rat, a retrograde fiber tracing method was combined with choline acetyltransferase immunohistochemistry. After injection of WGA-HRP into the superior colliculus, approximately 12% of retrogradely labeled cells in the substantia nigra were found to express choline acetyltransferase immunoreactivity. These double labeled cells were located in the dorsal and lateral portions of the substantia nigra pars reticulata at its middle and caudal levels.
Moriizumi
T
T
Department of Anatomy, Faculty of Medicine, University of Toronto, Ont. Canada.
Leduc-Cross
B
B
Hattori
T
T
D000818:Animals; D001370:Axonal Transport; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000166:cytology / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013378:Substantia Nigra / Q000033:anatomy & histology* / Q000166:cytology / Q000201:enzymology; D013477:Superior Colliculi / Q000033:anatomy & histology* / Q000166:cytology / Q000201:enzymology; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins
D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D006735:Horseradish Peroxidase; D002795:Choline O-Acetyltransferase