Effects of tachykinins on [3H]taurine release from human astrocytoma cells (U-373 MG).
Substance P (SP) stimulated [3H]taurine release from human astrocytoma cells (U-373 MG). This effect was concentration dependent and the EC50 was 0.3 nM. This stimulatory effect of SP can be inhibited by spantide, a SP receptor antagonist. The rank order of potencies of related tachykinins and their analogues in stimulating the release of [3H]taurine was SP much greater than neurokinin A much greater than neurokinin B and [Glp6,L-Pro9]SP (6-11) much greater than [Glp6, D-Pro9]SP (6-11) which conformed to that reported for the tachykinin NK-1 receptor. In addition to SP, isoproterenol, a beta-adrenergic agonist, can also increase the release of [3H]taurine from these cells and the effects of SP and isoproterenol were additive.
Tung
W L
WL
Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin.
Lee
C M
CM
D001254:Astrocytoma; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D015288:Neurokinin A / Q000494:pharmacology*; D013373:Substance P / Q000031:analogs & derivatives* / Q000494:pharmacology*; D015320:Tachykinins / Q000494:pharmacology*; D013654:Taurine / Q000378:metabolism*; D014316:Tritium
D015320:Tachykinins; D014316:Tritium; D013654:Taurine; D013373:Substance P; D015288:Neurokinin A; D000242:Cyclic AMP; D007545:Isoproterenol