Isolation of an SBA lectin-reactive glycoprotein (gp50) and its identification in Schistosoma mansoni larval and adult worm secretions.
A major 50-kDa soybean agglutinin (SBA)-reactive component present in extracts of Schistosoma mansoni eggs was isolated by SBA lectin affinity chromatography. In polyacrylamide gel electrophoresis (PAGE), the SBA-reactive component was seen as a 100-kDa polypeptide band that after reduction and alcylation was substituted by a 50-kDa component. This suggests that it occurs in native form as a dimer. Monoclonal antibodies produced against gp50 reacted with miracidial and cercarial secretions and with adult worm components including tegumental structures suggestive of a secretory function.
Linder
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National Bacteriological Laboratory, Department of Parasitology, Stockholm, Sweden.
Thors
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Lundin
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D015801:Helminth Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D037102:Lectins; D008099:Liver / Q000469:parasitology; D008297:Male; D008856:Microscopy, Fluorescence; D037121:Plant Lectins; D012550:Schistosoma mansoni / Q000032:analysis* / Q000254:growth & development / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000469:parasitology*; D030262:Soybean Proteins; D010835:Phytohemagglutinins
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D015801:Helminth Proteins; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins