title: [A method of determining heparin in blood plasma based on its antithrombin activity].
abstract: The suggested method for measuring blood plasma heparin is based on heparin ability to enhance antithrombin activity of antithrombin III (AT-III), the major Xa and thrombin inhibitor. The method consists in measurement of blood plasma AT-III activity in the presence and absence of protamine sulfate that destroys the heparin--AT-III complex. Heparin content in U/ml is determined from the difference in the activities of heparin--AT-III complex and AT-III proper activity represented on the calibration curve. The method is sufficiently sensitive, it permits registration of heparin concentrations in a wide band (from 0.01 U/ml to 0.75 U/ml of plasma).
authors.forename: R E
authors.initials: RE
authors.lastname: Taktashev
authors.forename: A V
authors.initials: AV
authors.lastname: Suvorova
authors.forename: V E
authors.initials: VE
authors.lastname: Miller
authors.forename: O I
authors.initials: OI
authors.lastname: Kolesnikova
mesh_terms: D000990:Antithrombin III / Q000378:metabolism*; D006493:Heparin / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D011479:Protamines
chemical_list: D007202:Indicators and Reagents; D011479:Protamines; D000990:Antithrombin III; D006493:Heparin
vernacular_title: Metod opredeleniia geparina v plazme krovi po ego antitrombinovoĭ aktivnosti.