title: Strategies for the genetic manipulation of Saccharomyces cerevisiae.
abstract: The budding yeast Saccharomyces cerevisiae is now widely used as a model organism in the study of gene structure, function, and regulation in addition to its more traditional use as a workhorse of the brewing and baking industries. In this article the plethora of methods available for manipulating the genome of S. cerevisiae are reviewed. This will include a discussion of methods for manipulating individual genes and whole chromosomes, and will address both classic genetic and recombinant DNA-based methods. Furthermore, a critical evaluation of the various genetic strategies for genetically manipulating this simple eukaryote will be included, highlighting the requirements of both the new and the more traditional biotechnology industries.
authors.affiliation: Biological Laboratory, University of Kent, Canterbury, England.
authors.forename: M F
authors.initials: MF
authors.lastname: Tuite
mesh_terms: D001709:Biotechnology; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D005818:Genetic Engineering; D005822:Genetic Vectors; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004271:DNA, Fungal