title: 17D yellow fever vaccine virus envelope protein expressed by recombinant baculovirus is antigenically indistinguishable from authentic viral protein.
abstract: We have constructed a recombinant baculovirus containing cloned DNA encoding the membrane and envelope (E) proteins of 17D yellow fever vaccine virus. Spodoptera frugiperda cells infected with this recombinant baculovirus produced a 66K protein which corresponded to the estimated size of the protein encoded by the cloned inserted DNA, and a 54K protein with the same molecular size as that of the authentic 17D yellow fever virus E protein. This recombinant 54K protein was labile, producing E protein-specific breakdown products (45K to 36K). Indirect immunofluorescence, using a panel of E protein-specific monoclonal antibodies, showed that the recombinant protein was presented both inside as well as on the surface of cells and was antigenically indistinguishable from the E protein of 17D yellow fever vaccine virus.
authors.affiliation: NERC Institute of Virology and Environmental Microbiology, Oxford, U.K.
authors.forename: S Y
authors.initials: SY
authors.lastname: Shiu
authors.forename: S
authors.initials: S
authors.lastname: Morikawa
authors.forename: A
authors.initials: A
authors.lastname: Buckley
authors.forename: S
authors.initials: S
authors.lastname: Higgs
authors.forename: V
authors.initials: V
authors.lastname: Karunakarannair
authors.forename: C
authors.initials: C
authors.lastname: Blachere
authors.forename: E A
authors.initials: EA
authors.lastname: Gould
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D016367:Baculoviridae / Q000235:genetics; D002213:Capsid / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005822:Genetic Vectors; D015151:Immunoblotting; D008970:Molecular Weight; D009036:Moths; D011994:Recombinant Proteins / Q000276:immunology*; D015183:Restriction Mapping; D014614:Vaccines, Synthetic; D014758:Viral Core Proteins / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins / Q000235:genetics; D014765:Viral Vaccines; D015005:Yellow fever virus / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D000939:Epitopes; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines