Corticotropin-releasing factor inhibition of substance P-induced vascular leakage in rats: possible sites of action.
Substance P (SP), 40 micrograms/kg SC, induced protein leakage in the skin, muscle, trachea and esophagus of the anesthetized rat as measured by Monastral blue B labeling of small blood vessels. CRF, 30 micrograms/kg SC, injected 30 min before SP, decreased the SP-induced dye leakage. To locate where CRF might act, autoradiographic studies of [125I]-CRF binding to esophageal segments were conducted and displaceable binding of [125I]-CRF to submucosal elements in the esophageal epithelium were revealed, suggesting that CRF acts on selective sites to reduce vascular leakage.
Gao
G C
GC
School of Public Health, University of California, Berkeley 94720.
Dashwood
M R
MR
Wei
E T
ET
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D002199:Capillary Permeability / Q000187:drug effects*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism / Q000494:pharmacology*; D004947:Esophagus / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D013373:Substance P; D003346:Corticotropin-Releasing Hormone