Spectrophotometric determination of amylase activity in coloured solutions.
A simple procedure for spectrophotometric determination of amylase activity in coloured solutions is described. Starch cross-linked with a bifunctional reagent in the presence of black drawing ink is used as an insoluble chromolytic substrate. The absorbances of reaction mixture filtrates are read in the near-infrared region (at 800-900 nm) where the majority of coloured substances does not absorb; on the contrary, black drawing ink released from the substrate during the action of amylase absorbs strongly even at these wavelengths.
Safarík
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I
Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Ceské Budĕjovice, Czechoslovakia.
D000681:Amylases / Q000032:analysis*; D003116:Color; D012996:Solutions; D013053:Spectrophotometry / Q000379:methods
D012996:Solutions; D000681:Amylases