Effect of chitosan on lingual hemostasis in rabbits with platelet dysfunction induced by epoprostenol.
Chitosan, a complex carbohydrate derivative of shellfish exoskeleton, is shown to enhance lingual hemostasis in rabbits treated with a known antagonist of platelet function, epoprostenol (prostacyclin or PGI2). Bleeding times were measured for bilateral (15 mm x 2 mm) tongue incisions in 10 New Zealand white rabbits. Using a randomized, blinded experimental design, one incision in each animal was treated with chitosan and the other was treated with control vehicle without chitosan. Extraoral bleeding and coagulation times were measured for each animal before, during, and after infusion of epoprostenol. Continuous infusion of epoprostenol increased mean systemic bleeding time 95%. In this platelet dysfunction animal model, lingual incisions receiving the experimental substance showed a 56% improvement in bleeding time in comparison with lingual incisions receiving control solution (P = .003).
Klokkevold
P R
PR
UCLA School of Dentistry, CHS-Section of Hospital Dentistry 90024-1668.
Subar
P
P
Fukayama
H
H
Bertolami
C N
CN
D000704:Analysis of Variance; D000818:Animals; D001760:Bleeding Time; D001780:Blood Coagulation Tests; D001791:Blood Platelet Disorders / Q000139:chemically induced / Q000188:drug therapy*; D002686:Chitin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D048271:Chitosan; D004195:Disease Models, Animal; D011464:Epoprostenol; D006490:Hemostatics / Q000627:therapeutic use*; D011817:Rabbits; D014059:Tongue / Q000098:blood supply*
D006490:Hemostatics; D002686:Chitin; D048271:Chitosan; D011464:Epoprostenol