Comparative quantitation of anti-viral titers of polyclonal human IgG and (Fab')2 preparations by an enzyme-linked immunosorbent assay (ELISA): pitfalls of using appropriate second antibodies.
When comparing anti-viral antibody titers of intravenous immunoglobulins (i.v.-Igs) comprising two 7S IgG preparations and one corresponding (Fab')2 product, the specificity of the second ab-peroxidase conjugate employed in the ELISA was found to be decisive for the experimental results. Abs specific for the whole IgG molecule, in contrast to the (Fab')2 specific ones, obviously contain populations recognizing epitopes localized on C gamma 2 and C gamma 3 domains, and, therefore, cause an underestimation of titers of the (Fab')2 preparation.
Gronski
P
P
Behringwerke AG., Marburg (Lahn), F.R.G.
Diderrich
G
G
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes; D005069:Evaluation Studies as Topic; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G