[Monoclonal antibodies obtained against G-protein epitopes: comparison of immunoreactions seen in the brain, retina and striated muscular tissue].
Monoclonal antibodies have been obtained against a purified fraction of brain G proteins containing the Gi alpha, G0 alpha, G beta, and G gamma subunits. After characterization, two monoclonal antibodies have been used to detect the cellular distribution of the two epitopes in neural, retinal and muscular tissues: ELISA, cross-dot and Western blot demonstrated that F.IV.5 is an anti-G beta antibody specific for the 36 kDa beta-subunit. ELISA, cross-dot and immunocytochemical distribution of the epitopes recognized by F.VII.9 suggested that this antibody recognizes epitopes which are also detected with polyclonal anti-G0 alpha antibodies. With both monoclonal antibodies, we confirmed that G proteins demonstrated a sub-membranous distribution as well as extensively cytoplasmic, axoplasmic or sarcoplasmic distributions in different cell types.
Peraldi-Roux
S
S
Laboratoire de Neurobiologie endocrinologique, U.R.A. n 1197, C.N.R.S., Université de Montpellier-II.
Mangeat
P
P
Rouot
B
B
Oliver
J
J
Herbute
S
S
Brabet
P
P
Homburger
V
V
Toutant
M
M
Bockaert
J
J
Gabrion
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000276:immunology*; D009412:Nerve Fibers / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000276:immunology*; D012160:Retina / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; C029152:G-substrate; D009419:Nerve Tissue Proteins
Obtention d'anticorps monoclonaux dirigés contre des épitopes de protéines G: comparaison des immunoréactions observées dans le cerveau, la rétine et le tissu musculaire strié.