Monocytes may amplify their recruitment into inflammatory lesions by inducing monocyte chemotactic protein.
By Northern analysis, freshly isolated monocytes contained no detectable mRNA for monocyte chemotactic protein-1 (MCP-1). However, after 4 hours of incubation at 37 degrees C, MCP-1 mRNA was clearly induced in the monocytes and was found to be highly dependent and directly proportional to the monocyte density. The level of MCP-1 mRNA continued to increase, reaching a peak after 22 hours of incubation. After 3 days in culture, MCP-1 mRNA levels had declined substantially and after 8 days were undetectable in the monocytes/macrophages. The amount of MCP-1 protein secreted correlated with the density-dependent increase in MCP-1 message. We hypothesize that the migration of monocytes into inflammatory lesions may be amplified by the density and time-dependent induction of MCP-1.
Cushing
S D
SD
Department of Medicine, UCLA School of Medicine 90024-1679.
Fogelman
A M
AM
D001483:Base Sequence; D002448:Cell Adhesion; D002452:Cell Count; D002454:Cell Differentiation; D002478:Cells, Cultured; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000096:biosynthesis* / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000473:pathology / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D015342:DNA Probes; D012333:RNA, Messenger