Affinity purification of human tau proteins and the construction of a sensitive sandwich enzyme-linked immunosorbent assay for human tau detection.
Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau protein was used to purify tau proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau proteins. The affinity-purified human tau was used to produce a high-titered rabbit anti-human tau serum. The monoclonal anti-tau antibody and the polyclonal rabbit anti-tau serum were then used to construct a sandwich enzyme-linked immunosorbent assay for detection of human tau proteins, with a sensitivity of 1 ng/ml.
Mercken
M
M
Innogenetics, Ghent, Belgium.
Vandermeeren
M
M
Lübke
U
U
Six
J
J
Boons
J
J
Vanmechelen
E
E
Van de Voorde
A
A
Gheuens
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007106:Immune Sera; D016875:tau Proteins / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D007106:Immune Sera; D016875:tau Proteins