Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
Nitric oxide is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. Cloning of a complementary DNA for brain nitric oxide synthase reveals recognition sites for NADPH, FAD, flavin mononucleotide and calmodulin as well as phosphorylation sites, indicating that the synthase is regulated by many different factors. The only known mammalian enzyme with close homology is cytochrome P-450 reductase.
Bredt
D S
DS
Department of Neuroscience, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205.
Hwang
P M
PM
Glatt
C E
CE
Lowenstein
C
C
Reed
R R
RR
Snyder
S H
SH
D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002147:Calmodulin / Q000378:metabolism; D002460:Cell Line; D002531:Cerebellum / Q000201:enzymology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005486:Flavin Mononucleotide / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000235:genetics*; D019001:Nitric Oxide Synthase; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution; D014162:Transfection
D002147:Calmodulin; D012333:RNA, Messenger; D005182:Flavin-Adenine Dinucleotide; D009249:NADP; D005486:Flavin Mononucleotide; D004247:DNA; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009251:NADPH-Ferrihemoprotein Reductase