Counteraction by nicardipine of the ionophoretic effect of gliclazide: a mitochondrial study.
Using mitochondria, we demonstrate that gliclazide exhibits a calcium ionophoretic activity. Indeed, gliclazide induces a decrease in the respiratory control of mitochondria; this effect is increased by addition of Ca2+ and corrected by ruthenium red, all characteristics of a calcium ionophoretic compound. Our results, on a biological membrane, confirm previous findings in vitro. Moreover, we show that nicardipine counteracts the action of gliclazide. Besides the effect on ATP-stimulated K+ channels, the ionophoretic effect of gliclazide may play a role in its hypoglycaemic effect, thus this counteracting action of nicardipine might induce drug interaction during the concomitant clinical administration of gliclazide and nicardipine.
Ubeaud
G
G
Laboratoire de Biophysique, Faculté de Pharmacie, Marseille, France.
Chauvet-Monges
A M
AM
Crevat
A
A
D000818:Animals; D002118:Calcium / Q000378:metabolism; D005907:Gliclazide / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D066298:In Vitro Techniques; D007478:Iontophoresis; D015636:Magnesium Chloride / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D009529:Nicardipine / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology
D015636:Magnesium Chloride; D012430:Ruthenium Red; D009529:Nicardipine; D005907:Gliclazide; D002118:Calcium