title: Actin molecules promote neurite outgrowth of chick telencephalic neurons in vitro.
abstract: Chick brain extract contains protein factors for neurite outgrowth from embryonic chick telencephalic neurons in dissociated cell cultures. In the course of purification of the factors using a bioassay system, a 41 kDa protein was detected to be one of the factors. Immunoblot analysis showed that this protein is identical to actin. In addition, G-actin purified from rabbit skeletal muscle exhibits neurite outgrowth activity in the same dose-dependent manner as the 41 kDa protein. The same bioassay carried with other purified proteins did not show such a remarkable activity.
authors.affiliation: Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nobusada
authors.forename: T
authors.initials: T
authors.lastname: Taguchi
mesh_terms: D000199:Actins / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008970:Molecular Weight; D016501:Neurites / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013687:Telencephalon / Q000502:physiology*
chemical_list: D000199:Actins