Nucleo-cytoplasmic incompatibility in cybrid plants possessing an Atropa genome and a Nicotiana plastome.
Twenty-nine cybrids possessing an Atropa belladonna nuclear genome and a Nicotiana tabacum plastome were selected from two independent protoplast fusion experiments. In contrast to the previously described reciprocal, green and fertile cybrids with a Nicotiana nuclear genome and an Atropa plastome (Kushnir et al. 1987), the plants obtained were totally chlorophyll-deficient. An Atropa nuclear genome and a Nicotiana plastome from these chlorophyll-deficient cybrids were combined with an Atropa or a Scopolia plastome and a Nicotiana nuclear genome, respectively, in control fusion experiments. All of these nuclear genome/plastome combinations gave rise to normal, green plants. Therefore, we conclude that an N. tabacum plastome is incompatible with an A. belladonna nuclear genome.
Kushnir
S
S
Institute of Cell Biology and Genetic Engineering, Ukrainian Academy of Sciences, Kiev, USSR.
Babiychuk
E
E
Bannikova
M
M
Momot
V
V
Komarnitsky
I
I
Cherep
N
N
Gleba
Y
Y
D000681:Amylases / Q000378:metabolism; D001532:Atropa belladonna / Q000032:analysis / Q000235:genetics*; D002459:Cell Fusion; D002467:Cell Nucleus / Q000378:metabolism*; D002734:Chlorophyll / Q000032:analysis; D003593:Cytoplasm / Q000378:metabolism*; D017343:Genes, Plant; D006822:Hybrid Cells; D007527:Isoenzymes / Q000378:metabolism; D010641:Phenotype; D010946:Plants, Medicinal; D010947:Plants, Toxic; D014026:Nicotiana / Q000032:analysis / Q000235:genetics*
D007527:Isoenzymes; D002734:Chlorophyll; D000681:Amylases