title: Drosophila Rrp1 protein: an apurinic endonuclease with homologous recombination activities.
abstract: A protein previously purified from Drosophila embryo extracts by a DNA strand transfer assay, Rrp1 (recombination repair protein 1), has an N-terminal 427-amino acid region unrelated to known proteins, and a 252-amino acid C-terminal region with sequence homology to two DNA repair nucleases, Escherichia coli exonuclease III and Streptococcus pneumoniae exonuclease A, which are known to be active as apurinic endonucleases and as double-stranded DNA 3' exonucleases. We demonstrate here that purified Rrp1 has apurinic endonuclease and double-stranded DNA 3' exonuclease, activities and carries out single-stranded DNA renaturation in a Mg(2+)-dependent manner. Strand transfer, 3' exonuclease, and single-stranded DNA renaturation activities comigrate during column chromatography. The properties of Rrp1 suggest that it could promote homologous recombination at sites of DNA damage.
authors.affiliation: Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
authors.forename: M
authors.initials: M
authors.lastname: Sander
authors.forename: K
authors.initials: K
authors.lastname: Lowenhaupt
authors.forename: A
authors.initials: A
authors.lastname: Rich
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004330:Drosophila / Q000201:enzymology / Q000235:genetics*; D029721:Drosophila Proteins; D004706:Endodeoxyribonucleases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000378:metabolism; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D029721:Drosophila Proteins; D029968:Escherichia coli Proteins; D009713:Nucleotidyltransferases; C061900:Rrp1 protein, Drosophila; D004706:Endodeoxyribonucleases; D043223:Deoxyribonuclease IV (Phage T4-Induced); C468972:endonuclease IV, E coli; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase