Microwave irradiation increases recovery of neuropeptides from brain tissues.
The effect of focused high energy microwave treatment (MW) on brain concentrations and molecular forms of substance P, neurokinin A, neuropeptide Y, neurotensin, galanin and calcitonin gene-related peptide was investigated. Groups of rats were treated as follows: 1) MW, storage for 60 min at 22 degrees C, 2) Decapitation, storage for 60 min at 22 degrees C. 3) Decapitation, storage for 60 min at 22 degrees C, MW treatment, 4) MW, decapitation, storage for 2 min at 22 degrees C and 5) Decapitation, storage for 2 min at 22 degrees C. Peptide concentrations were in all instances highest in the MW sacrificed groups. MW increased the concentration of intact peptides by rapid inhibition of peptidase activity and increase in peptide solubility/extractability.
Theodorsson
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E
Karolinska Institute, Department of Clinical Chemistry, Karolinska Hospital, Stockholm, Sweden.
Stenfors
C
C
Mathé
A A
AA
D000818:Animals; D001923:Brain Chemistry / Q000528:radiation effects*; D015740:Calcitonin Gene-Related Peptide / Q000528:radiation effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D019004:Galanin; D008297:Male; D008872:Microwaves; D015288:Neurokinin A / Q000528:radiation effects; D009478:Neuropeptide Y / Q000528:radiation effects; D009479:Neuropeptides / Q000032:analysis / Q000528:radiation effects*; D009496:Neurotensin / Q000528:radiation effects; D010455:Peptides / Q000528:radiation effects; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000528:radiation effects
D009478:Neuropeptide Y; D009479:Neuropeptides; D010455:Peptides; D013373:Substance P; D009496:Neurotensin; D015288:Neurokinin A; D019004:Galanin; D015740:Calcitonin Gene-Related Peptide