title: Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation.
abstract: Hematoxylin-eosin staining (H&E) of cytomegalovirus (CMV) inclusion bodies was compared with in situ hybridization using a biotinylated DNA probe for the detection of CMV. Of 29 biopsy specimens selected from 23 patients with clinical CMV hepatitis and typical CMV inclusions on histopathologic examination, 23 (79%) were positive by DNA probe and 17 (59%) were detected in cell cultures. The mean number of CMV foci per tissue section was higher by DNA probe (12) compared with H&E (5). We do not recommend in situ hybridization in microbiology laboratories as a replacement for histopathology for the diagnosis of CMV in tissue specimens.
authors.affiliation: Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905.
authors.forename: M J
authors.initials: MJ
authors.lastname: Espy
authors.forename: C V
authors.initials: CV
authors.lastname: Paya
authors.forename: K E
authors.initials: KE
authors.lastname: Holley
authors.forename: J
authors.initials: J
authors.lastname: Ludwig
authors.forename: P F
authors.initials: PF
authors.lastname: Hermans
authors.forename: R H
authors.initials: RH
authors.lastname: Wiesner
authors.forename: R A
authors.initials: RA
authors.lastname: Krom
authors.forename: T F
authors.initials: TF
authors.lastname: Smith
mesh_terms: D001707:Biopsy, Needle; D001710:Biotin; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D006525:Hepatitis, Viral, Human / Q000175:diagnosis*; D006801:Humans; D007181:Inclusion Bodies, Viral; D008099:Liver / Q000382:microbiology* / Q000473:pathology; D016031:Liver Transplantation; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D013194:Staining and Labeling
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D001710:Biotin