Normalization of protein synthesis in dystrophic neurons of cerebral cortex in rats after hypoxia, opening of the blood-brain barrier and treatment with organospecific RNA.
The possibility of normalization of protein synthesis intensity was explored in dystrophic neurons and in the total brain cortex of rats after acute hypoxic hypoxia. We avoided transplantation of embryonic nervous tissue (ENT) into the rat brain, as we did before, as well as operations and brain damages in opening of the blood-brain barrier (BBB) caused by hypoxia and intramuscular injections of organo (brain)-specific RNA. As shown by the autoradiographic and biochemical methods using radioactive isotopes (3H-leucine), hypoxia causes a statistically significant reduction in the intensity of protein synthesis which increases and becomes normalized after injection of brain-specific RNA into femoral muscles of animals. Thus, it is possible to normalize hypoxia-inhibited compensation-restoration processes in the brain cortex of animals and, probably, the function of the higher nervous activity using the new simple and harmless biological method. The data presented are of priority significance and important both for development of a number of fundamental biological problems and for medicine since the described method permits the treatment of some serious nervous and mental diseases in humans.
Polezhaev
L V
LV
N. I. Vavilov Institute of General Genetics, USSR Academy of Sciences, Moscow.
Cherkasova
L V
LV
Vitvitsky
V N
VN
Timonin
A V
AV
D000818:Animals; D001345:Autoradiography; D001812:Blood-Brain Barrier; D001927:Brain Diseases / Q000503:physiopathology* / Q000628:therapy; D002540:Cerebral Cortex / Q000503:physiopathology*; D000860:Hypoxia / Q000503:physiopathology*; D007273:Injections, Intramuscular; D007930:Leucine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000502:physiology*; D012313:RNA / Q000008:administration & dosage* / Q000235:genetics; D051381:Rats; D014316:Tritium
D009419:Nerve Tissue Proteins; D014316:Tritium; D012313:RNA; D007930:Leucine