Differences in metabolite content between intact pancreases and their perchloric acid extracts. A 2D 1H/31P correlation NMR study.
Perchloric and hydrochloric acid extracts of intact pancreases from healthy rats and from rats with experimental acute pancreatitis were analysed using 2D 1H/31P correlation NMR spectroscopy. Major differences were found in the 2D maps between the extracts and the intact tissues. In the case of the intact diseased pancreas the prominent 31P signal at the phosphodiester region has been assigned in our previous work as lecithin/taurocholate complex. However, the signal found in the same chemical shift region in extracts of diseased pancreases as well as healthy ones, is identified here as the phosphodiester residue of oligoribonucleotides. In these extracts additional 31P signals were found and assigned as phosphomonoester and phosphodiester hydrolysis products of RNA. The amounts of these compounds, as a function of the acid concentrations were determined, and conditions for their minimization were defined.
Askenasy
N
N
Sackler Faculty of Exact Sciences, School of Chemistry, Tel-Aviv University, Israel.
Kushnir
T
T
Navon
G
G
Kaplan
O
O
D000818:Animals; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D010184:Pancreatic Extracts / Q000378:metabolism*; D010195:Pancreatitis / Q000378:metabolism; D010472:Perchlorates; D010758:Phosphorus; D011522:Protons; D012313:RNA / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D010184:Pancreatic Extracts; D010472:Perchlorates; D011522:Protons; D010758:Phosphorus; D012313:RNA