[Detection of high interleukin 6 concentrations in miniaturized human bone marrow long-term cultures].
The long-term bone marrow culture system (LTBMC) consists of an inductive environment provided by a marrow-derived adherent layer which induces pluripotent stem cell replication. The activity of IL-6 was analyzed in micro LTBMC as a potential stem cell regulator. Immediately after initiation there was a tremendous increase in IL-6 activity. With each refeeding, IL-6 levels rose until confluency of the stromal layer was reached at week two to three post initiation. As a working hypothesis for further investigations, we suggest the immediate shift of stem cells after refeeding from a non-cycling into a cycling state might be mediated by the steep increase of IL-6 alone or in cooperation with other factors.
Mergenthaler
H G
HG
GSF-Institut für Experimentelle Hämatologie, Univ. Klinikum Grosshadern.
Hültner
L
L
Mempel
W
W
Dörmer
P
P
D016026:Bone Marrow Transplantation / Q000276:immunology*; D002460:Cell Line; D006410:Hematopoiesis / Q000502:physiology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D015850:Interleukin-6 / Q000502:physiology*
D015850:Interleukin-6
Nachweis von hohen Interleukin-6-Konzentrationen in miniaturisierten humanen Knochenmark-Langzeitkulturen.