The lack of mutagenicity of aryl 4-guanidinobenzoates in the transplacental micronucleus assay in mice.
Two acrosin inhibitors, 4'-methylumbelliferyl 4-guanidinobenzoate and 2'-carbomethoxyphenyl 4-guanidinobenzoate, were tested for mutagenicity in the transplacental micronucleus assay and the mouse bone marrow micronucleus assay. The compounds were administered intraperitoneally at doses of 125 mg/kg and 250 mg/kg to pregnant mice. Fetal peripheral blood and maternal bone marrow cells were examined at 36 h for the frequency of micronucleated polychromatic erythrocytes. Neither compound induced micronuclei in maternal or fetal tissues. The ratio of polychromatic erythrocytes to normochromatic erythrocytes was not affected by the drug treatments indicating that the compounds had no effect on the cell cycle or mitosis in these tissues and that they were not cytotoxic. Both compounds, which show promise as vaginal contraceptives, were not mutagenic in this study.
Curry
P T
PT
Department of Zoology and Physiology, University of Wyoming, Laramie 82071.
Ziemer
T
T
Waller
D P
DP
Zaneveld
L J
LJ
Kitchin
R M
RM
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D005333:Fetus / Q000166:cytology; D006146:Guanidines / Q000633:toxicity*; D006413:Hematopoietic System / Q000166:cytology / Q000187:drug effects; D006923:Hymecromone / Q000031:analogs & derivatives* / Q000633:toxicity; D051379:Mice; D015162:Micronucleus Tests; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity*; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D013194:Staining and Labeling
C017532:4-methylumbelliferylguanidinobenzoate; D006146:Guanidines; D009153:Mutagens; D006923:Hymecromone; C057430:2'-carbomethoxyphenyl 4-guanidinobenzoate