title: Glycosylation and transmembrane topography of bovine chromaffin granule p65.
abstract: The bovine homologue of p65, a calmodulin-binding protein located in the membranes of synaptic vesicles and endocrine secretory granules, has been studied by the use of monoclonal antibodies directed against this antigen and against dopamine beta-mono-oxygenase. The protein (apparent molecular mass 67 kDa; pI = 5.5-6.2) is partially degraded by treatment with neuraminidase or endoglycosidase F. Trypsin treatment of intact adrenal chromaffin granules or of granule membranes releases a soluble 39 kDa fragment of p65 which corresponds to the whole of its cytoplasmic domain. This domain contains both the epitope for the monoclonal antibody cgm67 and the calmodulin-binding site. The 20 amino acids at the N-terminus of this fragment are identical to part of the rat p65 sequence.
authors.affiliation: Department of Biochemistry, University of Edinburgh Medical School, Scotland, U.K.
authors.forename: H B
authors.initials: HB
authors.lastname: Tugal
authors.forename: F
authors.initials: F
authors.lastname: van Leeuwen
authors.forename: D K
authors.initials: DK
authors.lastname: Apps
authors.forename: J
authors.initials: J
authors.lastname: Haywood
authors.forename: J H
authors.initials: JH
authors.lastname: Phillips
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000737:chemistry* / Q000276:immunology; D002417:Cattle; D002837:Chromaffin Granules / Q000737:chemistry* / Q000378:metabolism; D003598:Cytoskeletal Proteins; D003600:Cytosol / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006031:Glycosylation; D007425:Intracellular Membranes / Q000737:chemistry* / Q000378:metabolism; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: D002148:Calmodulin-Binding Proteins; D003598:Cytoskeletal Proteins; D000939:Epitopes; D008840:Microfilament Proteins; D010750:Phosphoproteins; C066559:Lcp1 protein, mouse