Multipotent and committed CD34+ cells in bone marrow transplantation.
In order to study the role of CD34+ cells in hematological recovery following bone marrow transplantation (BMT), bone marrow cells stained with HPCA-1 (CD34) and MY-9 (CD33) monoclonal antibodies were analyzed by using a fluorescence-activated cell sorter on or about days 14 and 28, as well as at later times, following BMT in 6 recipients. Single cell cultures of CD34+ cells were also performed to evaluate their in vitro hematopoietic function. CD34+ cells were detectable in bone marrow cells on day 14. More than 80% of CD34+ cells co-expressed the CD33 antigen, and macrophage (Mac) colony-forming cells predominated among total colony-forming cells of CD34+ cells. In normal bone marrow cells, CD34+, CD33+ cells amounted to about 40% of CD34+ cells, and the incidences of erythroid bursts, granulocyte/macrophage (GM) colonies, and Mac colonies were similar to each other. After more than 10 weeks, CD34+, CD33- cells gradually recovered, as erythroid burst colony-forming cells increased following GM colony-forming cells. This phenomenon was well-correlated with the time course of peripheral blood cell recovery. CD34+, CD33+ cells as committed progenitors and CD34+, CD33- cells as multipotent stem cells have distinctive biological behaviors in BMT.
Ema
H
H
Department of Medicine, Jichi Medical School, Tochigi-ken.
Suda
T
T
Nakauchi
H
H
Nakamura
Y
Y
Iwama
A
A
Imagawa
S
S
Akutsu
M
M
Kano
Y
Y
Kato
S
S
Yabe
M
M
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D016026:Bone Marrow Transplantation; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D013997:Time Factors
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34