Inhibition of influenza A virus hemagglutinin and induction of interferon by synthetic sialylated glycoconjugates.
Multivalent forms of neoglycoproteins and polyacrylamides containing sialic acid were prepared and shown to be potent inhibitors of influenza A virus (H3N2) hemagglutinin with chick red blood cells. The synthetic sialylated glycoconjugates, although they were neuraminidase substrates, did not suppress viral neuraminidase and did not reduce infectivities in chick embryos. The copolyacrylamide conjugate containing a spacer group of approximately 11 A (1 A = 0.1 nm) between the polymer backbone and the sialic acid residues was the best hemagglutinin inhibitor. Moreover, it exhibited promising interferon-inducing properties.
Gamian
A
A
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.
Chomik
M
M
Laferrière
C A
CA
Roy
R
R
D000818:Animals; D002642:Chick Embryo; D005786:Gene Expression Regulation / Q000187:drug effects; D006001:Glycoconjugates / Q000737:chemistry / Q000494:pharmacology*; D006387:Hemagglutination, Viral / Q000187:drug effects; D006389:Hemagglutinins, Viral / Q000037:antagonists & inhibitors*; D009980:Influenza A virus / Q000187:drug effects / Q000502:physiology*; D007372:Interferons / Q000096:biosynthesis*; D051379:Mice; D008815:Mice, Inbred Strains; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000378:metabolism; D012794:Sialic Acids / Q000494:pharmacology*
D006001:Glycoconjugates; D006389:Hemagglutinins, Viral; D012794:Sialic Acids; D007372:Interferons; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid