[The structure and function of botulinum type C neurotoxin].
The structure gene for botulinum type C neurotoxin was cloned from the toxigenic bacteriophage obtained from Clostridium botulinum type C, and the whole nucleotide sequence was determined. The nucleotide sequence contained a single open reading frame coding for 1,291 amino acids corresponding to a polypeptide with a molecular weight of 149,000. The signal peptide was not found after the first methionine residue. Upstream of the ATG codon, sequences predicted as a Shine-Dalgarno and a promoter were found. When the deduced amino acid sequence of type C toxin was compared with those of type A and D botulinum toxins and tetanus toxin, type C toxin shared about 52% identity with type D toxin, but shared only about 33% identity with type A and tetanus toxins. The structure and function of type C toxin were estimated from the results of epitope map with monoclonal antibodies and DNA thermal stability map.
Kimura
K
K
Department of Microbiology, Sapporo Medical College, Japan.
D000595:Amino Acid Sequence; D001483:Base Sequence; D001905:Botulinum Toxins / Q000737:chemistry / Q000235:genetics; D003062:Codon; D004269:DNA, Bacterial; D000939:Epitopes; D008969:Molecular Sequence Data; D008970:Molecular Weight
D003062:Codon; D004269:DNA, Bacterial; D000939:Epitopes; D001905:Botulinum Toxins