Three types of bovine chromaffin cell Ca2+ channels: facilitation increases the opening probability of a 27 pS channel.
1. Cell-attached patch recordings from bovine chromaffin cells were performed with 90 mM-Ba2+ in the patch pipette and with isotonic potassium aspartate in the bathing solution to zero the membrane potential. Three different types of unitary Ca2+ channel activity could be distinguished in these recordings. 2. A 27 pS Ca2+ channel was distinguished by constructing amplitude histograms and measuring slope conductance. This channel activated over a broad range of potentials (depolarizations greater than -10 mV). 3. A second Ca2+ channel with a slope conductance of 14 pS could also be detected with amplitude histograms. This channel activated with depolarizations greater than -20 mV. 4. An 18 pS Ca2+ channel was observed infrequently indicating that this channel may carry only a small amount of the whole-cell current. This 18 pS channel was sensitive to changes in holding potential. Depolarizing the patch to +10 mV from a holding potential of -80 mV elicited robust unitary activity. Changing the patch holding potential to -40 mV while maintaining test depolarizations to +10 mV completely inactivated the 18 pS channel. Neither the 25 pS nor the 14 pS Ca2+ channels were affected by changes in holding potential in the range from -80 mV to -40 mV, indicating the 18 pS channel was a different type of channel. As the 18 pS channel was observed so infrequently, no detailed studies of it were possible. 5. Chromaffin cell Ca2+ currents exhibited facilitation. Large pre-depolarizations greatly augmented whole-cell currents observed in these cells. Whole-cell currents could double or triple after recruiting facilitation. The application of large pre-depolarizations altered the gating behaviour of the 27 pS Ca2+ channel manifested as dramatically increased channel opening probabilities measured during subsequent test pulses. Large pre-depolarizations induced unitary activity in the 27 pS Ca2+ channel similar to the long-lived openings exhibited by L-type Ca2+ channels in the presence of Bay K 8644. Large pre-depolarizations did not change the gating behaviour of the 14 pS Ca2+ channel. 6. Repetitive depolarizations in the physiological range could also induce facilitation. At the single-channel level facilitation was manifested as a striking increase in opening probability of the 27 pS Ca2+ channel. No effect of repetitive activity was observed on 14 pS channel gating. At the whole-cell level, repetitive depolarizations dramatically increased the current observed. 7. Facilitation of 27 pS Ca2+ channel activity could be induced by changing the holding potential to a depolarized level (greater than or equal to -10 mV).(ABSTRACT TRUNCATED AT 400 WORDS)
Artalejo
C R
CR
Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637.
Mogul
D J
DJ
Perlman
R L
RL
Fox
A P
AP
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D002838:Chromaffin System / Q000166:cytology / Q000502:physiology*; D004558:Electric Stimulation; D015737:Nisoldipine / Q000494:pharmacology
D015220:Calcium Channels; D015737:Nisoldipine; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester