The effect of bleomycin on the uptake and incorporation of [14C]choline into phospholipids in hamster lung tissue slices.
Intratracheal administration of the anticancer drug bleomycin to hamsters produced an increase in the uptake and incorporation of [14C]choline into phospholipids of lung slices in vitro. The stimulatory effect is opposite to the results obtained previously using [14C]acetate and would appear to occur distal to cytidine diphosphocholine. Although alternate explanations are possible, the results are consistent with morphological evidence, published by others, indicating an increase in lung phospholipid following bleomycin treatment, and illustrate the significance of precursor selection when evaluating the effects of xenobiotics on phospholipid synthesis.
Hollinger
M A
MA
Department of Pharmacology, School of Medicine, University of California, Davis 95616.
Giri
S N
SN
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002794:Choline / Q000378:metabolism*; D006224:Cricetinae; D006909:Hydroxyproline / Q000032:analysis; D066298:In Vitro Techniques; D008168:Lung / Q000737:chemistry*; D008297:Male; D008647:Mesocricetus; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000378:metabolism*
D010713:Phosphatidylcholines; D010743:Phospholipids; D001761:Bleomycin; D002794:Choline; D006909:Hydroxyproline