title: Antibody to histo-blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood group B or O donors.
abstract: Three virus isolates HTLV-IIIB/lyA, HTLV-IIIB/lyB and HTLV-IIIB/lyO, obtained by passaging and propagating the HTLV-IIIB/H9 isolate in three separate cultures of mixed peripheral blood mononuclear cells (PBMC) from donors of blood type A, B or O, respectively, were tested for susceptibility for virus neutralization by the monoclonal antibody (MAb) AH16 directed against the blood group A epitope. MAb AH16 was previously shown to inhibit cell-free virus infection using HTLV-IIIB propagated in H9 cells. AH16 showed a concentration-dependent inhibition of the HTLV-IIIB/lyA isolate but did not inhibit the HTLV-IIIB/lyB or the HTLV-IIIB/lyO isolate. Specificity of the MAb-mediated inhibition was shown using A-antigen (tetrasaccharide). Thus, HIV infection of PBMC from donors with blood type A appears to induce expression of host-cell-encoded carbohydrate blood group A epitope on HIV which can be a target for MAb-mediated virus neutralization.
authors.affiliation: Department of Infectious Diseases 144, Hvidovre Hospital, University of Copenhagen, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Arendrup
authors.forename: J E
authors.initials: JE
authors.lastname: Hansen
authors.forename: H
authors.initials: H
authors.lastname: Clausen
authors.forename: C
authors.initials: C
authors.lastname: Nielsen
authors.forename: L R
authors.initials: LR
authors.lastname: Mathiesen
authors.forename: J O
authors.initials: JO
authors.lastname: Nielsen
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001782:Blood Donors; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D000939:Epitopes; D006031:Glycosylation; D006678:HIV / Q000254:growth & development / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D009500:Neutralization Tests
chemical_list: D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D015488:HIV Antigens