Expression of hematopoietic growth factor RNAs in human mesenchymal cells from various organs.
Experiments were undertaken to study expression of hematopoietic growth factor RNAs in mesenchymal cells from a variety of organs including bone marrow, foreskin, gingiva, and lung. Cells from each organ had negligible expression of RNAs coding for granulocyte (G), macrophage (M), and granulocyte-macrophage (GM) colony stimulating factor (CSF), interleukin 1 beta (IL-1 beta), and IL-6. Fibroblasts from each tissue had a comparable ability to express the same cytokine RNAs. Surprisingly, the stimuli for expression of G-CSF RNA was disparate from the stimuli for expression of the other cytokine RNAs. While IL-1 beta enhanced accumulation of G-CSF RNA, tumor necrosis factor alpha (TNF) and 12-O-tetradecanoylphorbol-13-acetate (TPA) did not. In contrast, IL-1 beta, TNF, and TPA equally stimulated increased levels of M-CSF, GM-CSF, IL-1 beta and IL-6 RNAs.
Yamato
K
K
Department of Medicine, UCLA School of Medicine, Cedars-Sinai Medical Centre 90024.
el-Hajjoui
Z
Z
Koeffler
H P
HP
D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D016298:Hematopoietic Cell Growth Factors / Q000235:genetics*; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000494:pharmacology; D015850:Interleukin-6 / Q000235:genetics; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics; D012313:RNA / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D016298:Hematopoietic Cell Growth Factors; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013755:Tetradecanoylphorbol Acetate