Unique use of alternative polyadenylation signals in the mouse aldolase B gene.
We isolated and sequenced two mouse aldolase B cDNAs. They differ only in the length of the 3' untranslated region. This is consistent with Northern blot analysis of liver RNA which shows two transcripts differing by 400 nucleotides. We also isolated and sequenced the corresponding 3' genomic region and found four polyadenylation signals in the final exon. RNase protection studies demonstrate that all four of these signals are utilized, but not equally. This is unique to the mouse aldolase B gene.
Maine
A B
AB
Department of Biochemistry, University of Rochester School of Medicine and Dentistry, NY 14642.
Stauffer
J K
JK
Tolan
D R
DR
Ciejek-Baez
E
E
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D015698:Genomic Library; D007527:Isoenzymes / Q000235:genetics; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics*; D012333:RNA, Messenger / Q000302:isolation & purification
D007527:Isoenzymes; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA; D005634:Fructose-Bisphosphate Aldolase