Some monoclonal anti-human lymphocyte and class II MHC antigen antibodies do not stain snap frozen Macaca fascicularis lymphocytes or tissue sections.
Experiments were designed to stain snap frozen and fixed Macaca fascicularis peripheral blood mononuclear cell (PBMC) preparations and colon sections assuming that monoclonal mouse anti-human lymphocyte and class II major histocompatibility complex antigen antibodies would cross-react with the monkey counterparts to the human antigens. Most of the monoclonals used in this study did not stain the monkey frozen and fixed tissues in immunoenzymatic protocols despite adequate staining of control human tissues. The fact that snap frozen Macaca fascicularis PBMCs and tissue sections were not always stained is of practical importance for experimental design in Macaca fascicularis using anti-human reagents.
Li
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Department of Clinical Bacteriology, Karolinska Institute, Stockholm, Sweden.
Forsum
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D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D003106:Colon / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005615:Freezing; D005629:Frozen Sections; D006681:HLA-D Antigens / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008252:Macaca fascicularis / Q000097:blood / Q000276:immunology*; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000939:Epitopes; D006681:HLA-D Antigens