title: Acridine orange stain for determining intracellular enteropathogens in HeLa cells.
abstract: Green-fluorescent intracellular enteropathogenic bacteria were observed after infected HeLa cell monolayers were stained with acridine orange and counterstained with crystal violet at least 3 h after infection.
authors.affiliation: Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
authors.forename: M D
authors.initials: MD
authors.lastname: Miliotis
mesh_terms: D000165:Acridine Orange; D004755:Enterobacteriaceae / Q000648:ultrastructure*; D004756:Enterobacteriaceae Infections / Q000175:diagnosis*; D005456:Fluorescent Dyes; D005840:Gentian Violet; D006367:HeLa Cells / Q000382:microbiology*; D006801:Humans; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D000165:Acridine Orange; D005840:Gentian Violet