Ultrastructural identification of trigeminal nerve terminals in the pterygopalatine ganglion of rats: an anterograde tracing and immunohistochemical study.
Trigeminal nerve terminals in the rat pterygopalatine ganglion (PPG) were ultrastructurally identified using anterograde tracing with Phaseolus vulgaris-leucoagglutinin (PHA-L). Electron microscopic immunohistochemistry was used to demonstrate the presence of substance P (SP) and calcitonin gene-related peptide (CGRP) in nerve terminals of the PPG. Adjacent to the rostral part of the PPG an additional minor area was described. Perikarya in this minor rostral part were more spherical and had irregular outlines. Ultrastructurally, the glial enwrapment of the nerve terminals seemed to be more loosely arranged in comparison to that in the major rostral part of the PPG. With PHA-L, numerous labelled nerve fibres and terminals were found in all parts of the PPG. The ultrastructure of these terminals was uniform, many of them showing synaptic contacts. Numerous terminals in the PPG were SP-positive, whereas only a few were CGRP-positive. Fibres stained positive for both neuropeptides. The PPG is shown to be synaptically innervated by sensory fibres arising in the trigeminal ganglion, with the strong suggestion of SP and CGRP acting as neurotransmitters. A modulatory interaction between the autonomic and sensory system, resembling an axon reflex mechanism in the peripheral nervous system is endorsed.
Beckers
H J
HJ
The Netherlands Ophthalmic Research Institute, Department of Morphology, Amsterdam.
Klooster
J
J
Vrensen
G F
GF
Lamers
W P
WP
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism / Q000502:physiology; D005726:Ganglia, Parasympathetic / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008297:Male; D009411:Nerve Endings / Q000648:ultrastructure*; D010835:Phytohemagglutinins; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000378:metabolism / Q000502:physiology; D014276:Trigeminal Nerve / Q000648:ultrastructure*
D010835:Phytohemagglutinins; C100156:leukoagglutinins, plants; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide