title: Complementary DNA cloning and nucleotide sequence of rabbit serum amyloid A protein.
abstract: A complementary DNA clone encoding serum amyloid A protein has been isolated from an acute rabbit liver cDNA library. Complete nucleotide sequence analysis reveals that the cloned gene contains a 24 bases 5' untranslated region, 369 bases coding region and a 106 bases 3' untranslated region. Primer extension analysis indicates that the full-length 5' untranslated region contains 80 nucleotides. Northern blot analysis of mRNA from normal and acute rabbit livers demonstrates that this gene is expressed constitutively at a low level and undergoes induction of transcription in response to acute inflammation by the administration of turpentine.
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Medicine, University of Missouri, Columbia 65211.
authors.forename: B K
authors.initials: BK
authors.lastname: Ray
authors.forename: A
authors.initials: A
authors.lastname: Ray
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D008099:Liver / Q000502:physiology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D011817:Rabbits; D000685:Serum Amyloid A Protein / Q000235:genetics*
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D000685:Serum Amyloid A Protein; D011061:Poly A; D012313:RNA; D004247:DNA