title: Molecular aspects of the neuronal noradrenaline transporter.
abstract: The neuronal noradrenaline transporter was partially purified by means of low and high pressure liquid chromatography using anion exchange, gel filtration and lectin affinity columns. A protein characterized by a molecular weight of 50-53 kilodalton was enriched; it may represent the transporter or a component of it. In addition, a RNA fraction characterized by a mean size of 2 kilobases was isolated from PC12 rat phaeochromocytoma cells and from bovine adrenal medulla; this RNA fraction caused expression of the noradrenaline transporter after microinjection into Xenopus laevis oocytes.
authors.affiliation: Department of Pharmacology and Toxicology, University of Bonn, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Bönisch
authors.forename: G
authors.initials: G
authors.lastname: Paulus
authors.forename: G
authors.initials: G
authors.lastname: Martiny-Baron
authors.forename: B
authors.initials: B
authors.lastname: Lingen
authors.forename: D
authors.initials: D
authors.lastname: Coppeneur
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003891:Desipramine / Q000378:metabolism / Q000494:pharmacology; D007488:Iprindole / Q000494:pharmacology; D008376:Maprotiline / Q000031:analogs & derivatives / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism*; D050484:Norepinephrine Plasma Membrane Transport Proteins; D009865:Oocytes / Q000378:metabolism*; D016716:PC12 Cells; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D027981:Symporters; D014982:Xenopus laevis
chemical_list: D002352:Carrier Proteins; D050484:Norepinephrine Plasma Membrane Transport Proteins; D012333:RNA, Messenger; D027981:Symporters; D011061:Poly A; D008376:Maprotiline; C027299:hydroxymaprotilin; D012313:RNA; D007488:Iprindole; D003891:Desipramine; D009638:Norepinephrine