Comparison of neutralization of BPV-1 infection of C127 cells and bovine fetal skin xenografts.
BPV-1 induces focus formation in murine C127 cells and fibropapillomas in bovine fetal skin xenografts. In this study, we compared the specificity of neutralization of BPV-1 in both assay systems, using sera and monoclonal antibodies (MAbs) selected to define neutralizing epitopes. Sera from rabbits and cattle, inoculated with intact BPV-1 or PBV-2 virions, neutralize BPV-1 infectivity in both C127 cells and xenografts. Selected human sera and murine MAbs that react with intact BPV-1 particles, serum of a rabbit immunized with denatured BPV-1 particles, and sera from calves vaccinated with a recombinant LI fusion protein did not neutralize BPV-1 infection in either system. It was concluded that neutralization of BPV-1 infection of C127 cells and bovine fetal skin xenografts by hyperimmune sera is specific and concordant for both assay systems, and involves conformational BPV-1 capsid epitopes.
Ghim
S
S
Department of Pathology, Georgetown University Medical School, Washington, DC 20007.
Christensen
N D
ND
Kreider
J W
JW
Jenson
A B
AB
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology*; D005260:Female; D051379:Mice; D009500:Neutralization Tests; D011817:Rabbits; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000276:immunology*
D000939:Epitopes