Probing the structure and function of the nuclear pore complex.
Nucleocytoplasmic transport is a bi-directional process mediated by the nuclear pore complex (NPC), which results in a segregation of cytoplasmic and nuclear macromolecules within cells. Some progress has been made in understanding the mechanistic basis of this selective transport phenomenon. In particular, cryo-electron microscopy of frozen-hydrated nuclear envelopes coupled with image processing and labeling studies, has provided a glimpse of the transporter at the center of the NPC.
Akey
C W
CW
Department of Biophysics, Boston University School of Medicine, MA 02118-2394.
D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D016616:Cryoultramicrotomy / Q000379:methods*; D007089:Image Enhancement; D008854:Microscopy, Electron / Q000379:methods*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009337:Necturus / Q000033:anatomy & histology; D009685:Nuclear Envelope / Q000648:ultrastructure*; D013194:Staining and Labeling; D014981:Xenopus / Q000033:anatomy & histology