Release of mediators from human gastric mucosa and blood in adverse reactions to benzoate.
A study was carried out on 29 patients to investigate the amount of histamine liberation and release of platelet-activating factor and 6-keto-prostaglandin F1 alpha from gastric mucosa and whole blood or mononuclear cells by sodium benzoate. The patients suffered from asthma (10), atopic dermatitis (7) and chronic urticaria (4). 8 patients with unrelated, non-immunologic diseases served as controls. In the oral provocation test (OPT) 3 patients experienced a recurrence of their original disease, whilst 1 asthmatic patient reacted with abdominal disorder. The release of histamine and prostaglandin from mucosa was significantly increased by sodium benzoate in comparison to the spontaneous release observed in patients. The mucosa of the control persons did not react to sodium benzoate. Furthermore, there was a significant difference in prostaglandin release between patients with positive OPT and the control persons. No difference could be found between patients with negative OPT and those with positive OPT. Additionally, in the mediator release from whole blood or mononuclear cells there was no obvious difference apparent. These results suggest a possible involvement of prostacyclin and histamine in adverse reactions to benzoate. Due to the sensitivity of the method, a mediator release from mucosa can already be demonstrated in a preclinical state of the pseudoallergic reaction in the absence of clinical symptoms.
Schaubschläger
W W
WW
Medizinische Klinik, Forschungsinstitut Borstel, BRD.
Becker
W M
WM
Schade
U
U
Zabel
P
P
Schlaak
M
M
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis; D000293:Adolescent; D000328:Adult; D000368:Aged; D001249:Asthma / Q000276:immunology; D001565:Benzoates / Q000009:adverse effects* / Q000494:pharmacology; D019817:Benzoic Acid; D003876:Dermatitis, Atopic / Q000276:immunology; D016099:Endoscopy, Gastrointestinal; D005260:Female; D005503:Food Additives / Q000009:adverse effects*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D006632:Histamine / Q000032:analysis; D006636:Histamine Release; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010972:Platelet Activating Factor / Q000032:analysis; D014581:Urticaria / Q000276:immunology
D001565:Benzoates; D005503:Food Additives; D010972:Platelet Activating Factor; D015121:6-Ketoprostaglandin F1 alpha; D006632:Histamine; D019817:Benzoic Acid