Adhesion of human cancer cells to vascular endothelium mediated by a carbohydrate antigen, sialyl Lewis A.
Recently the lectin-like domain on ELAM-1 (endothelial leukocyte adhesion molecule-1) was shown to recognize a carbohydrate antigen, sialyl Lewis x. In this paper we demonstrate, by a series of inhibition experiments utilizing specific monoclonal antibodies and pure glycolipid preparations, that the sialyl Lewis a antigen serves as a specific ligand for ELAM-1 as well as sialyl Lewis x and plays a significant role in the ELAM-1-mediated binding of human cancer cells to activated endothelial cells.
Takada
A
A
Department of Laboratory Medicine, Kyoto University, School of Medicine, Japan.
Ohmori
K
K
Takahashi
N
N
Tsuyuoka
K
K
Yago
A
A
Zenita
K
K
Hasegawa
A
A
Kannagi
R
R
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D018395:CA-19-9 Antigen; D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism; D004337:Drug Carriers; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen / Q000378:metabolism; D008024:Ligands; D008081:Liposomes; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D018395:CA-19-9 Antigen; D015815:Cell Adhesion Molecules; D004337:Drug Carriers; D019040:E-Selectin; D005732:Gangliosides; D007375:Interleukin-1; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; D008024:Ligands; D008081:Liposomes; D011994:Recombinant Proteins; C064564:sialyl Le(a) ganglioside