Cholera toxin- and forskolin-induced cyclic AMP accumulations of pig skin (epidermis). Modulation by chemicals which reveal the beta-adrenergic augmentation effect.
Effects of cholera toxin and forskolin on pig epidermal adenylate cyclase system were investigated. Both agents increased cyclic AMP levels of epidermis. Marked accumulations were observed in the presence of cyclic AMP phosphodiesterase inhibitor, isobutylmethylxanthine (IBMX). It has been known that hormone-stimulated adenylate cyclase responses are modified by various chemical treatments. Following long term incubation with hydrocortisone, Ro10-1670, and colchicine, the epidermal beta-adrenergic adenylate cyclase response was increased without the alteration of cyclic AMP phosphodiesterase activity. Adenosine-, and histamine-adenylate cyclase responses were unchanged by hydrocortisone treatment, and were decreased by Ro10-1670 and colchicine treatments. Following the long term incubation with these chemicals, effects of cholera toxin and forskolin were investigated. Colchicine-treated skin revealed the increased cholera toxin-, and forskolin-induced cyclic AMP accumulations. Neither hydrocortisone- nor Ro10-1670-treated skin revealed alterations of cholera toxin-, and forskolin-effect. The stimulatory effect of colchicine on the cholera toxin-, and forskolin-effect was observed at doses of the beta-adrenergic augmentation effect. Our results indicate that among the chemicals which reveal the beta-adrenergic augmentation effect, colchicine is unique in that it also increases cholera toxin-, and forskolin-induced cyclic AMP accumulations of epidermis.
Matsuo
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S
Department of Dermatology, Asahikawa Medical College, Japan.
Iizuka
H
H
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D017255:Acitretin; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology*; D003078:Colchicine / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D004817:Epidermis / Q000201:enzymology; D005938:Glucocorticoids / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D012176:Retinoids / Q000494:pharmacology; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*; D014212:Tretinoin / Q000031:analogs & derivatives / Q000494:pharmacology
D005938:Glucocorticoids; D012176:Retinoids; D005576:Colforsin; D014212:Tretinoin; D002772:Cholera Toxin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D017255:Acitretin; D003078:Colchicine; D015056:1-Methyl-3-isobutylxanthine; D006854:Hydrocortisone