Evolution of the "titin epitope" in neurofilament proteins.
1. The specificity of a monoclonal antibody raised to human titin was characterized. The antibody reacts with an epitope which is common to titin and the high mol. wt subunits NF-H and NF-M of mammalian neurofilaments. 2. Mapping of the epitope indicated that it is located in the carboxyterminal extension of NF-H and NF-M, and that its reactivity does not depend on the phosphorylation state of the molecule. 3. A comparative study on neurofilament protein of lower vertebrates revealed that this epitope has been conserved during vertebrate evolution.
Mencarelli
C
C
Department of Evolutionary Biology, University of Siena, Italy.
Magi
B
B
Marzocchi
B
B
Armellini
D
D
Pallini
V
V
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D005075:Biological Evolution; D064211:Connectin; D000939:Epitopes / Q000276:immunology*; D005399:Fishes; D006801:Humans; D015151:Immunoblotting; D009124:Muscle Proteins / Q000276:immunology*; D016900:Neurofilament Proteins / Q000276:immunology*; D011494:Protein Kinases; D012104:Reptiles; D014714:Vertebrates
D000911:Antibodies, Monoclonal; D064211:Connectin; D000939:Epitopes; D009124:Muscle Proteins; D016900:Neurofilament Proteins; C577285:TTN protein, human; D011494:Protein Kinases