title: Humoral immune response of tuberculous patients against the three components of the Mycobacterium bovis BCG 85 complex separated by isoelectric focusing.
abstract: An isoelectric-focusing technique followed by Western blot (immunoblot) analysis was used to investigate the immunoglobulin G response of tuberculous patients against each of the three components of the Mycobacterium bovis BCG antigen 85 complex. The 85A component was stained by the tuberculous as well as the non-tuberculous sera. In contrast, the 85B and the 85C proteins of the complex were not stained by the control sera but were stained by 20 of 28 tuberculous serum samples.
authors.affiliation: Chest Department, Hospital Erasme, Université Libre de Bruxelles, Belgium.
authors.forename: J P
authors.initials: JP
authors.lastname: Van Vooren
authors.forename: A
authors.initials: A
authors.lastname: Drowart
authors.forename: M
authors.initials: M
authors.lastname: De Cock
authors.forename: A
authors.initials: A
authors.lastname: Van Onckelen
authors.forename: M H
authors.initials: MH
authors.lastname: D'Hoop
authors.forename: J C
authors.initials: JC
authors.lastname: Yernault
authors.forename: C
authors.initials: C
authors.lastname: Valcke
authors.forename: K
authors.initials: K
authors.lastname: Huygen
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000302:isolation & purification; D015153:Blotting, Western; D000939:Epitopes; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007525:Isoelectric Focusing; D008875:Middle Aged; D009163:Mycobacterium bovis / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes; D007074:Immunoglobulin G