Characterization of two monoclonal antibodies against teguments of adult and schistosomula of Schistosoma japonicum.
Two monoclonal antibodies (McAbs) were produced from BALB/c mice hyperimmunized with tegumental extract of Schistosoma japonicum (Chinese strain). The two McAbs were characterized with regard to antibody isotype, antigen binding specificity and parasite stage specificity. One McAb, 8G9-5, was identified as IgM, whereas the other McAb, 9E7, was determined to be IgG2a. Immunoblotting assay indicated that McAb 8G9-5 binds strongly to the band of tegumental antigens of Mr 64 kDa but also binds weakly to other bands at 116, 105, 97, 54, 50, 47 and 45 kDa, whereas 9E7 McAb reacts specifically at Mr 54 kDa. Anatomical localization of the antigens in the adult worm by indirect immunofluorescence assay indicated that the target epitopes of McAb 8G9-5 are in the intra-tegumental structure, whereas the McAb 9E7 epitope is on the surface membrane. The two McAbs also react at similar sites within the teguments of schistosomula and lung worms.
Viyanant
V
V
Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Sobhon
P
P
Upatham
E S
ES
Kusamran
T
T
Kittigul
C
C
Panuwatsuk
W
W
Ardseungnoen
P
P
Anatawara
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D012549:Schistosoma japonicum / Q000033:anatomy & histology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes