Assessment of human cytotoxic T cell activity using synthetic peptides: potential for field application.
The use of synthetic peptides to delineate epitopes recognized by major histocompatability complex-restricted cytotoxic T lymphocytes has been well documented. In the present study, we report a method for the generation and assay of peptide-specific cytotoxic T lymphocytes adapted for the field situation where quantities of blood are often limited. From a single bleed of the donor and using minimal quantities of peptide (less than 5 micrograms/ml), up to 100 potential epitopes could be screened within a short time frame. Subsequent cloning of positive cultures for further analysis of defined epitopes is permissible. The system is readily adaptable to a wide range of viral, parasitic or bacterial infections.
Doolan
D L
DL
Tropical Health Program, Queensland Institute of Medical Research, Herston, Brisbane, Australia.
Houghten
R A
RA
Good
M F
MF
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D000939:Epitopes; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D000939:Epitopes; D010455:Peptides; D011948:Receptors, Antigen, T-Cell