title: Secreted or nonsecreted forms of acidic fibroblast growth factor produced by transfected epithelial cells influence cell morphology, motility, and invasive potential.
abstract: Addition of exogenous acidic fibroblast growth factor (aFGF) to NBT-II epithelial carcinoma cells results in fibroblastic transformation and cell motility. We have generated aFGF-producing NBT-II cells by transfection with recombinant expression vectors containing human aFGF cDNA, or the human aFGF cDNA coupled to a signal peptide (SP) sequence. The effects of the nonsecreted and the secreted 16-kDa growth factor on the morphology, motility, and cell invasive potential (gelatinase activity) were compared. aFGF coupled to a SP was actively secreted out of the producing cells. The secretion of aFGF was not necessary for induction of gelatinase activity, as this was observed in NBT-II cells producing aFGF with or without SP. Production of aFGF, whether secreted or not secreted, resulted in increased in vitro motility of most isolated clones; however, there was no correlation between aFGF level and motility rate. The data suggest that expression of aFGF in NBT-II cells induces metastatic potential through an autocrine or intracrine mechanism.
authors.affiliation: Laboratoire de Physiopathologie du Développement, Centre National de la Recherche Scientifique Unite de Recherche Associeé 1337, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Jouanneau
authors.forename: J
authors.initials: J
authors.lastname: Gavrilovic
authors.forename: D
authors.initials: D
authors.lastname: Caruelle
authors.forename: M
authors.initials: M
authors.lastname: Jaye
authors.forename: G
authors.initials: G
authors.lastname: Moens
authors.forename: J P
authors.initials: JP
authors.lastname: Caruelle
authors.forename: J P
authors.initials: JP
authors.lastname: Thiery
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D002465:Cell Movement; D003001:Cloning, Molecular; D016220:Fibroblast Growth Factor 1 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015183:Restriction Mapping; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000473:pathology / Q000503:physiopathology
chemical_list: D012333:RNA, Messenger; D016220:Fibroblast Growth Factor 1