[The antigenic structure of glycoprotein E2 in the Venezuelan equine encephalomyelitis virus studied using rat monoclonal antibodies].
A collection of 21 rat hybridomas secreting high-affinity monoclonal antibodies to Venezuelan equine encephalomyelitis (VEE) virus was generated. Using a panel of 15 monoclonal antibodies to glycoprotein E2, the antigenic structure of this protein of VEE strains TC-83 and 230 was studied. A competitive radioimmunoassay suggested a new map of the antigenic structure of glycoprotein E2 in which 5 sites including 11 epitopes of monoclonal antibody binding were distinguished. Antibody to E2-2 site neutralized virus infectivity and blocked hemagglutination test and antibody to E2-3 site could only block hemagglutination. Antibodies to other E2 protein sites lacked any biological activity.
Razumov
I A
IA
Agapov
E V
EV
Pereboev
A V
AV
Protopopova
E V
EV
Lebedeva
S D
SD
Loktev
V B
VB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000956:Antigens, Viral / Q000032:analysis*; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D009500:Neutralization Tests; D011863:Radioimmunoassay; D051381:Rats; D014759:Viral Envelope Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D014759:Viral Envelope Proteins
Izuchenie antigennoĭ struktury glikoproteida E2 virusa venesuél'skogo éntsefalomielita loshadeĭ s pomoshch'iu krysinykh monoklonal'nykh antitel.