Loss of basal cell phenotype with acquisition of lung-colonizing capability in mouse mammary tumors.
A transplantable pregnancy-dependent mouse mammary tumor, TPDMT-4, and its ovarian-dependent (T4-OR26) and autonomous (T4-OI96, T4-OI145, T4-OI165, T4-OI320 and T4-OI320CY) sublines were examined immunohistochemically for the expression of keratin 14 and type IV collagen. T4-OI96, T4-OI145, and T4-OI165, but not T4-OR26, T4-OI320, or T4-OI320CY, formed lung colonies (metastasis) after intravenous injection as a single-cell suspension. Despite the similar morphology of TPDMT-4 and its six sublines, only TPDMT-4 and the nonmetastatic sublines revealed a basal cell phenotype as defined by keratin 14 expression. Staining for type IV collagen was complete at the peripheries of the glandular structures in TPDMT-4 and nonmetastatic sublines but was patchy in the metastatic tumors.
Tsubura
A
A
Department of Pathology, Kansai Medical University, Osaka, Japan.
Inui
T
T
Morii
S
S
Dairkee
S H
SH
Oikawa
T
T
Matsuzawa
A
A
D000818:Animals; D003094:Collagen / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis*; D008175:Lung Neoplasms / Q000556:secondary*; D008325:Mammary Neoplasms, Experimental / Q000737:chemistry / Q000473:pathology*; D051379:Mice; D010641:Phenotype; D014407:Tumor Cells, Cultured
D007633:Keratins; D003094:Collagen