Phenotyping of T-cell lymphomas in paraffin sections--which antibodies?
Five antibodies, MT1 (CD43), UCHL1 (CD45RO), OPD4, poly-CD3 and beta F1, were assessed for their reactivity with 50 archival cases of T-cell lymphoma in formalin-fixed paraffin-embedded tissue. All cases had been previously characterized as T-cell lymphomas, and the histological types included 14 cases of small cerebriform lymphoma, six cases of angioimmunoblastic lymphadenopathy-like T-cell lymphoma, four cases of T-zone lymphoma, five cases of pleomorphic small cell lymphoma, 12 cases of pleomorphic medium and large cell lymphoma, four cases of anaplastic large cell lymphoma, two cases of T-lymphoblastic lymphoma and three cases of enteropathy-associated T-cell lymphoma. UCHL1 and MT1 showed reactivity with the highest percentage of cases (94 and 86% respectively) but lack absolute specificity for T-cells, especially in high-grade lymphomas. Poly-CD3 is highly specific for T-cells, and stained neoplastic cells in almost 80% of the cases. beta F1 stained the lowest percentage of cases (40%). UCHL1 and poly-CD3 together identified 98% of cases, and this combination is recommended for the diagnosis of T-cell lymphomas in paraffin sections.
Cabeçadas
J M
JM
Department of Histopathology, University College and Middlesex School of Medicine, London, UK.
Isaacson
P G
PG
D000906:Antibodies; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D016399:Lymphoma, T-Cell / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D010232:Paraffin; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D016610:Tissue Embedding
D000906:Antibodies; D010232:Paraffin