Hydrophobia, lectin binding, and molecular order in the stratum corneum of adult and neonatal rats and in human breast cells in culture.
A search for differences due to ANS staining (hydrophobia), Con A and PNA binding capacity, and birefringence was carried out on stratified epithelia of rat skin and human breast cells (HBC) in culture. Microfluorimetric measurements confirm that the ANS fluorescence of the stratum corneum from adults is higher than that of newborns. HBC exhibited an unexpected deep ANS-fluorescence. Differences in the binding capacity of the epithelial layers to Con A and PNA were detected with advancing age. Retardation measurements revealed that the form birefringence of the stratum corneum is higher in adult animals specially as revealed by the fact that its form birefringence curve branch from n = 1.414 to n = 1.479 is steeper, i.e. depict higher values. The strong birefringence of the cytoplasmic tonofilaments presented by cultured human breast cells was considered an unexpected finding and attributed to changes that the cells underwent following the in vitro conditions.
Vidal
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BC
Department of Cell Biology, Biology Institute, UNICAMP Campinas (SP) Brazil.
de Carvalho
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HF
D000328:Adult; D000704:Analysis of Variance; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology*; D010367:Arachis; D001718:Birefringence; D001940:Breast / Q000166:cytology* / Q000378:metabolism; D002478:Cells, Cultured; D003208:Concanavalin A / Q000378:metabolism; D003592:Cytophotometry; D005260:Female; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D019887:Peanut Agglutinin; D037121:Plant Lectins; D051381:Rats / Q000033:anatomy & histology*; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D013194:Staining and Labeling
D000817:Anilino Naphthalenesulfonates; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D003208:Concanavalin A