A lectin-binding soluble factor released by CD8+CD57+ lymphocytes from AIDS patients inhibits T cell cytotoxicity.
CD8+CD57+ T cells, expanded in peripheral blood lymphocytes of AIDS patients, inhibit the effector phase of HLA-specific cytotoxic T lymphocytes, natural killer and lymphocyte-activated killer cells in a 4-h chromium-release assay. This inhibitory activity present in supernatants of purified sorted CD8+CD57+ cells is mediated by a non-antigen-specific inhibitory factor which is distinct from prostaglandin E2, T cell growth factor (TGF)-beta, latent-TGF-beta, tumor necrosis factor (TNF)-alpha and TNF-beta. Partial biochemical characterization demonstrates that the CD8+CD57+ inhibitory activity (a) is heat, trypsin and acid resistant, (b) binds to concanavalin A columns, indicating its glycosylation state and (c) is mediated by a 20-30-kDa soluble molecule.
Sadat-Sowti
B
B
Laboratoire d'Immunologie Cellulaire et Tissulaire, CNRS UA 0186, CHU Pitié-Salpétrière, Paris.
Debré
P
P
Idziorek
T
T
Guillon
J M
JM
Hadida
F
F
Okzenhendler
E
E
Katlama
C
C
Mayaud
C
C
Autran
B
B
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D018959:CD57 Antigens; D016827:CD8 Antigens; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D037102:Lectins / Q000378:metabolism; D008970:Molecular Weight; D012995:Solubility; D013491:Suppressor Factors, Immunologic / Q000737:chemistry / Q000502:physiology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D018959:CD57 Antigens; D016827:CD8 Antigens; D037102:Lectins; D013491:Suppressor Factors, Immunologic