Identification of an immunodominant epitope within the phosphoprotein of rabies virus that is recognized by both class I- and class II-restricted T cells.
Immunization of H-2k mice with live rabies virus induces cytolytic T lymphocytes to the phosphoprotein of rabies virus. The antigenic determinant responsible for stimulating this class I-restricted cytolytic response was mapped to 50 amino acids (residues 180 to 229) of the phosphoprotein by using vaccinia virus recombinants expressing either the full-length phosphoprotein or C-terminal truncations of the phosphoprotein. The epitope was more finely mapped to residues 191 to 206 by using synthetic peptides. Several CD4+, class II-restricted T-cell lines were isolated from splenocytes of H-2k mice immunized with the vaccinia virus-rabies virus phosphoprotein recombinant virus. These lines were specifically stimulated by the phosphoprotein, and in addition, each line proliferated and released lymphokines in response to the same synthetic peptide shown to stimulate phosphoprotein-specific, class I-restricted cytolytic T cells.
Larson
J K
JK
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104-4268.
Wunner
W H
WH
Otvos
L
L
Ertl
H C
HC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D002872:Chromosome Deletion; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins / Q000235:genetics / Q000276:immunology*; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology*; D017361:Viral Nonstructural Proteins
D000939:Epitopes; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins