title: Electrophoretic transport of solutes in aqueous two-phase systems.
abstract: Electrophoretic transport of proteins across the interface between the phases of an aqueous polymer two-phase system can be greatly impeded in comparison with transport within the individual phase. This effect can be controlled by modifying the affinity of the protein for a phase by suitable manipulations of such variables as pH. The effect is not caused by differences in the electrophoretic velocity between the two phases, nor by large changes in pH at the interface. An analogy exists between this phenomenon and the related subject of diffusion of electrolytes across the phase interface.
authors.affiliation: Department of Chemical Engineering, University of Arizona, Tucson.
authors.forename: M L
authors.initials: ML
authors.lastname: Levine
authors.forename: M
authors.initials: M
authors.lastname: Bier
mesh_terms: D002021:Buffers; D004396:Coloring Agents; D003911:Dextrans; D004058:Diffusion; D004586:Electrophoresis; D005914:Globins / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009211:Myoglobin / Q000737:chemistry; D011092:Polyethylene Glycols; D012709:Serum Albumin / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D002021:Buffers; D004396:Coloring Agents; D003911:Dextrans; D006454:Hemoglobins; D009211:Myoglobin; D012709:Serum Albumin; D011092:Polyethylene Glycols; D005914:Globins