Use of riboprobes for northern blotting analysis.
We compared cDNA and riboprobes for their use in Northern blotting analysis and found that higher hybridization signal can be obtained using riboprobes. Comparison of five nylon membranes for Northern blotting using riboprobe revealed that each nylon membrane differed in their performance. Background noise was minimal for all five membranes for two riboprobes. However, because of the higher thermal stability of RNA.RNA hybrids, the riboprobes could not be stripped out from the membrane even after five hours of treatment at 80 degrees C.
Srivastava
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Dept. of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110.
Schonfeld
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G
D000818:Animals; D016632:Apolipoprotein A-I / Q000032:analysis; D001054:Apolipoproteins A / Q000032:analysis; D015152:Blotting, Northern / Q000379:methods*; D003001:Cloning, Molecular; D015342:DNA Probes; D008567:Membranes, Artificial; D051379:Mice; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000302:isolation & purification; D015347:RNA Probes
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D015342:DNA Probes; D008567:Membranes, Artificial; D015347:RNA Probes; C022205:apolipoprotein A-IV; D012313:RNA