title: Shiverer jimpy double mutant mice. V. Correlation of genotype and myelin proteins.
abstract: We have reexamined the levels of myelin basic protein (MBP) and proteolipid protein (PLP) in the brains of mice bred to carry both the shi/shi and jp/Y hypomyelination defects. The genotype of each putative double mutant was confirmed by direct DNA analysis: shi/shi by Southern blot analysis, and jp/Y by restriction enzyme analysis of polymerase chain reaction-amplified fragments. MBP and PLP levels were assessed by immunoblotting. All putative double mutants were found to be shi/shi. However, examination of the PLP locus revealed both jp and wild-type genotypes, the latter produced by an expected crossover. Animals proven to be shi/shi*jp/Y had no detectable MBP or PLP; those proven to be shi/shi*+/Y (the crossover) had no MBP but had PLP. These results differ from an earlier report of both MBP and PLP in the brains of presumed shi*jp animals.
authors.affiliation: Department of Neurology, Childrens Hospital, Boston, Mass.
authors.forename: A
authors.initials: A
authors.lastname: Sinclair
authors.forename: Y
authors.initials: Y
authors.lastname: Raz
authors.forename: D A
authors.initials: DA
authors.lastname: Kirschner
authors.forename: L
authors.initials: L
authors.lastname: Villa-Komaroff
authors.forename: M K
authors.initials: MK
authors.lastname: Wolf
authors.forename: S
authors.initials: S
authors.lastname: Billings-Gagliardi
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D001923:Brain Chemistry; D003434:Crossing Over, Genetic; D004252:DNA Mutational Analysis; D005838:Genotype; D051379:Mice; D008816:Mice, Jimpy / Q000235:genetics* / Q000378:metabolism; D008818:Mice, Neurologic Mutants / Q000235:genetics* / Q000378:metabolism; D004676:Myelin Basic Protein / Q000172:deficiency* / Q000235:genetics; D009185:Myelin Proteins / Q000032:analysis* / Q000235:genetics; D018991:Myelin Proteolipid Protein; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein