CD5+ B-lymphocytes are as dependent on T-helper cells and as responsive to T-suppressor cells in pokeweed mitogen-stimulated activation as conventional CD5- B-lymphocytes.
Pokeweed mitogen (PWM)-stimulated cultures were established with varying numbers of CD4+ or CD8+ cells and CD5+ immunoglobulin-secreting cells were subsequently identified using a combination of haemolytic plaque formation and rosetting with immunobeads coated with anti-Leu-1. It was found that CD5+ and CD5- B-cells required similar numbers of CD4+ cells for half-maximum plaque-forming cell (PFC) induction and that 50% suppression of both CD5+ and CD5- PFC occurred with similar numbers of CD8+ cells. Suppression of both CD5+ and CD5- PFC could occur when T-helper signals were supplied by a PWM-stimulated culture supernatant, indicating that both subsets could be directly suppressed by CD8+ cells rather than by indirect suppression of T-helper cells. It is concluded that CD5+ B-cells are as responsive to T-regulatory influences as conventional CD5- B-cells.
Jones
B M
BM
Department of Pathology, University of Hong Kong, Queen Mary Hospital.
D000328:Adult; D000943:Antigens, Differentiation / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D001402:B-Lymphocytes / Q000276:immunology*; D015704:CD4 Antigens / Q000494:pharmacology; D018956:CD5 Antigens; D016827:CD8 Antigens; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D006462:Hemolytic Plaque Technique; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000502:physiology*; D008211:Lymphocyte Cooperation / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012397:Rosette Formation; D006377:T-Lymphocytes, Helper-Inducer / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*
D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D018956:CD5 Antigens; D016827:CD8 Antigens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011043:Pokeweed Mitogens