Detection and identification of endothelin-1 immunoreactivity in rat and porcine thyroid follicular cells.
Endothelin-1 immunoreactivity (irET-1) was observed in rat and porcine thyroid glands. Using a radioimmunoassay for endothelin-1, the mean concentration in extracts of rat and porcine thyroid glands were 0.75 pg/mg +/- 0.03 (n = 4) and 1.5 pg/mg +/- 0.2 (n = 8) (mean +/- SE) respectively. Gel-filtration and reverse-phase HPLC showed that ir ET-1 eluted in a position identical to synthetic endothelin-1. In addition, immunohistochemical study showed that irET-1 is located within epithelial follicular cells. No immunostaining was seen in parafollicular C-cells nor in parathyroid.
Colin
I
I
Unité de Diabétologie et Nutrition, Catholic University of Louvain, Medical School, Brussels, Belgium.
Berbinschi
A
A
Denef
J F
JF
Ketelslegers
J M
JM
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D007150:Immunohistochemistry; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine; D013961:Thyroid Gland / Q000737:chemistry*
D016232:Endothelins