Immunohistochemical detection of a monoclonal antibody directed against the NGF receptor in basal forebrain neurons following intraventricular injection.
It has been shown by autoradiography that, following intraventricular administration, a monoclonal antibody directed against the rat nerve growth factor (NGF) receptor is specifically accumulated bilaterally by numerous cholinergic neurons of the basal forebrain. This is consistent with the evidence that cholinergic basal forebrain neurons have NGF receptors and respond to NGF under a variety of experimental conditions. The present study demonstrates that the immunohistochemical detection of unmodified monoclonal antibody in cholinergic forebrain neurons following transport from CSF is feasible, although injection of larger amounts of the antibody is required to obtain an image equivalent to the one obtained with the autoradiographic method. The location of the immunohistochemical product clearly indicates that the antibody has been internalized, probably in an endosomal compartment.
Thomas
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LB
Department of Pathology Division of Neuropathology, University of Tennessee, Memphis 38163.
Book
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Schweitzer
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JB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001345:Autoradiography; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry; D005260:Female; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D007457:Iodine Radioisotopes; D009474:Neurons / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000276:immunology*; D017475:Receptors, Nerve Growth Factor; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor