Epitope analysis of an HLA-B27-derived synthetic peptide: a possible approach to analyzing HLA class I antigens.
A monoclonal antibody, F3H7, was generated by immunizing mice with a synthetic peptide corresponding to residues 63-84 of the B*2705 allele of the HLA-B27 antigens. The reactive epitope and the contact residues on the peptide were localized by ELISA using a large panel of overlapping peptides as well as peptides with substituted amino acids. Residues corresponding to R75, D77 and L78 on the HLA-B27 protein appeared to be critical. The clarity of these results indicate that this is a potentially useful approach to the study of HLA class I epitopes.
Zhao
Y P
YP
Dept. of Surgery, Union Capital Hospital, Beijing, China.
Shi
B J
BJ
Deng
H K
HK
Yu
D T
DT
Hamachi
M
M
Hamachi
T
T
Zhao
D F
DF
Tang
S R
SR
Tsang
J C
JC
Park
M S
MS
D000483:Alleles; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D015796:HLA-B27 Antigen / Q000032:analysis / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000939:Epitopes; D015796:HLA-B27 Antigen; D015395:Histocompatibility Antigens Class I