title: Transport of 4-hydroxyphenylacetic acid in Klebsiella pneumoniae.
abstract: Klebsiella pneumoniae M5a1 has been shown to possess an inducible transport system for 4-hydroxyphenylacetate (4-HPA). This transport system has a Kt of 16.3 microM and a maximal velocity of 31.2 nmol/min (milligrams dry weight). The transport system has been inhibited by inhibitors of energy metabolism with a concomitant decrease in cellular ATP concentrations, and the 4-HPA binding activity has been detected in the crude shock extracts. All these observations indicate that 4-HPA uptake is an active transport which involves a periplasmic binding protein and it seems to be energized by phosphate bond energy.
authors.affiliation: Departamento de Bioquímica y Biologiá Molecular IV, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Allende
authors.forename: A
authors.initials: A
authors.lastname: Gibello
authors.forename: M
authors.initials: M
authors.lastname: Martin
authors.forename: A
authors.initials: A
authors.lastname: Garrido-Pertierra
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001692:Biological Transport; D002250:Carbon Radioisotopes; D004734:Energy Metabolism; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism*; D011865:Radioisotope Dilution Technique
chemical_list: D002250:Carbon Radioisotopes; D010648:Phenylacetates; C008070:4-hydroxyphenylacetic acid; D000255:Adenosine Triphosphate