title: Comparison of the antigenic peptides between human prostatic and lysosomal acid phosphatases.
abstract: The relative antigenicity (capacity to bind antibodies raised against the intact prostatic acid phosphatase) of the selected peptides from human prostatic and lysosomal acid phosphatases was evaluated in a competitive assay. Both prostatic and lysosomal acid phosphatases were shown to possess similar antigenic determinants on both terminal regions, along with more similarity on NH2-terminal peptide than COOH-terminal site. At least one additional antigenic site is present at the internal region of prostatic acid phosphatase, since the mixture of both amino- and carboxyl-terminal peptides exhibited only 70% inhibition.
authors.affiliation: Department of Diagnostic Immunology Research and Biochemistry, Roswell Park Memorial Institute, Buffalo, New York 14263.
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.forename: C L
authors.initials: CL
authors.lastname: Lee
authors.forename: S S
authors.initials: SS
authors.lastname: Li
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D001666:Binding Sites, Antibody; D000939:Epitopes / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D046911:Macromolecular Substances; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D011467:Prostate / Q000201:enzymology*; D014357:Trypsin
chemical_list: D000939:Epitopes; D046911:Macromolecular Substances; D010446:Peptide Fragments; D000135:Acid Phosphatase; D014357:Trypsin