The characteristics of antigen define the cellular source and kinetics of histamine-releasing factor induced by human peripheral-blood mononuclear cells.
Peripheral-blood leucocytes from Dermatophagoides pteronyssinus- and/or Lolium perenne-allergic patients produce in vitro histamine-releasing factor (HRF) in an allergen-specific and concentration-dependent relationship. Maximum production of HRF occurred in cultures containing as little as 10-100 pg/ml crude allergen extract, although a second peak occurred at higher concentrations (10-100 micrograms/ml). While HRF was detectable in 1-hour cultures, maximal production required 24 h in culture. In contrast, HRF induced by streptokinase/streptodornase (SK/SD) was generally maximal after 1 h. Allergen-induced HRF production was almost exclusively associated with monocytes and B cells. In contrast, peripheral-blood T cells were the major source of induced HRF production in cultures containing SK/SD. Histamine-releasing cytokines are apparently produced by different cell populations, the activation of which may be dependent upon the characteristics of the stimulating antigen.
Turner
K J
KJ
Department of Microbiology, University of Western Australia, Nedlands.
Strickland
D
D
Siemensma
N P
NP
Holt
B J
BJ
D000485:Allergens / Q000276:immunology*; D014408:Biomarkers, Tumor; D002478:Cells, Cultured; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D006967:Hypersensitivity / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008222:Lymphokines / Q000096:biosynthesis*; D000090324:Tumor Protein, Translationally-Controlled 1
D000485:Allergens; D014408:Biomarkers, Tumor; D008222:Lymphokines; D000090324:Tumor Protein, Translationally-Controlled 1