title: Detection of B19 parvovirus in human fetal tissues by electron microscopy.
abstract: We present the electron microscopy observations on samples from 38 pregnancies that were investigated for B19 parvovirus infection. Thirty-four had resulted in fetal loss thought to be due to a virus infection and 22 of the 38 were positive for B19 parvovirus in one or more of the tissues. Twenty-one placentas and 75 fetal tissue samples were examined. Fresh samples were investigated by immune electron microscopy while formalin-fixed tissues were examined as thin sections and by negative staining of tissue extracts with direct electron microscopy. Electron microscopy was more sensitive on fresh than on fixed samples. The ultrastructural observations on thin sections of fixed tissues yielded new information locating B19 parvovirus particles in both nucleus and cytoplasm of infected fetal cells. The diagnostic results of the range of electron microscopy assays were compared with those of two hybridization methods. The fresh samples yielded comparable results from electron microscopy and hybridisation assays but on formalin-fixed materials hybridisation was more sensitive.
authors.affiliation: Virus Reference Laboratory, Central Public Health Laboratory, Colindale, London, England.
authors.forename: A M
authors.initials: AM
authors.lastname: Field
authors.forename: B J
authors.initials: BJ
authors.lastname: Cohen
authors.forename: K E
authors.initials: KE
authors.lastname: Brown
authors.forename: J
authors.initials: J
authors.lastname: Mori
authors.forename: J P
authors.initials: JP
authors.lastname: Clewley
authors.forename: J P
authors.initials: JP
authors.lastname: Nascimento
authors.forename: N F
authors.initials: NF
authors.lastname: Hallam
mesh_terms: D016731:Erythema Infectiosum / Q000382:microbiology* / Q000473:pathology; D005260:Female; D005315:Fetal Diseases / Q000382:microbiology*; D005333:Fetus / Q000382:microbiology / Q000648:ultrastructure; D006801:Humans; D016253:Microscopy, Immunoelectron; D016732:Parvovirus B19, Human / Q000302:isolation & purification / Q000648:ultrastructure*; D010920:Placenta / Q000382:microbiology* / Q000648:ultrastructure; D011247:Pregnancy; D013194:Staining and Labeling