title: Trichinella spiralis: influence of an immunodominant, carbohydrate-associated determinant on the host antibody response repertoire.
abstract: Host antibody responses to the G2.1 epitope, a carbohydrate-associated determinant shared by several Trichinella spiralis glycoproteins, were examined by competitive inhibition enzyme-linked immunosorbent assay (ELISA). The G2.1 epitope dominated the AKR/J mouse antibody response whether the antigens were injected or introduced through infection, as determined by the serum blocking ability of a G2.1 epitope-specific monoclonal antibody (mAb). Serum T. spiralis-binding activity from several other infected mouse strains was blocked 22 to 86% by the G2.1 epitope-specific mAb. In addition to mice, the G2.1 epitope evoked powerful antibody responses in four other species. The binding activity of Trichinella-reactive antibodies from infected rats and pigs was inhibited 56 and 34%, respectively, by the mAb. Greater than 48% of the T. spiralis serum-binding activity from 4/5 infected humans was G2.1-specific. Most of the rabbit antibody response induced by injection of a previously characterized 43-kDa antigen was also directed to the G2.1 determinant. The specificities of 10 T. spiralis-reactive mAb were examined, and 7 reacted with the immunodominant epitope. Finally, of nine helminth species examined, only T. spiralis and T. pseudospiralis extracts efficiently blocked G2.1-specific antibody binding to solid-phase antigens. These results suggest that the responses to G2.1 epitope may play an important role during infection.
authors.affiliation: Department of Pathobiological Sciences, University of Wisconsin School of Veterinary Medicine 53706.
authors.forename: E Y
authors.initials: EY
authors.lastname: Denkers
authors.forename: C E
authors.initials: CE
authors.lastname: Hayes
authors.forename: D L
authors.initials: DL
authors.lastname: Wassom
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology*; D002241:Carbohydrates / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes; D006376:Helminths / Q000276:immunology; D006801:Humans; D007114:Immunization; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008815:Mice, Inbred Strains; D011817:Rabbits; D051381:Rats; D013552:Swine; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D002241:Carbohydrates; D000939:Epitopes