Isolation of two forms of decay-accelerating factor (DAF) from human urine.
Decay-accelerating factor (DAF) was purified from human pooled urine by conventional techniques. The urine DAF was separated into two peaks, pool I and pool II, by gel chromatography. DAF-U1 was isolated from pool I by hydrophobic chromatography, and DAF-U2 from pool II by anti-DAF IgG column. The specific activities of DAF-U1 and DAF-U2 to decay membrane-phase C5 convertase were about 3% and 70% of membrane form DAF, respectively. However, both urine DAFs revealed a similar activity to each other and slightly higher activity than that of membrane form DAF in decay-accelerating fluid-phase C3 convertase of the alternative pathway.
Nakano
Y
Y
Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Sugita
Y
Y
Ishikawa
Y
Y
Choi
N H
NH
Tobe
T
T
Tomita
M
M
D000595:Amino Acid Sequence; D018958:CD55 Antigens; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D050577:Complement C3-C5 Convertases / Q000037:antagonists & inhibitors; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine; D008969:Molecular Sequence Data
D018958:CD55 Antigens; D008565:Membrane Proteins; D050577:Complement C3-C5 Convertases