[Microdot immunoenzyme analysis of chorionic gonadotropin in pregnancy].
A microdot enzyme immunoassay test system has been developed for measuring chorionic gonadotropin levels in urine samples, on the basis of affine purified antibodies to hormonal beta-subunit and antibody complex with horse radish peroxidase. The results are assessed visually with the use of a color indicator scale obtained in experiments with reference preparations containing 12.5, 50, and 200 mMU of the hormone per ml. Comparison of the intensity of microdot color permits characterizing the level of hormone secretion and diagnose pregnancy 4-5 days before the first day of expected menstruation (50 mMU/ml). The specificity, reproducibility, and sensitivity of the developed test system are not inferior to common enzyme immunoassay on the plates; the method does not involve the use of sophisticated equipment and is reagent-saving. The diagnostic accuracy of the test system is 100 percent.
Vlasov
G S
GS
Rudik
G M
GM
Kuz'mina
N S
NS
Krasnoproshina
L I
LI
D006063:Chorionic Gonadotropin / Q000652:urine*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D011247:Pregnancy; D011258:Pregnancy Tests
D006063:Chorionic Gonadotropin
Mikrotochechnyĭ immunofermentnyĭ analiz khorionicheskogo gonadotropina pri beremennosti.