Characterisation of the Salmonella O:8 antigen in the O-chain of the lipopolysaccharide produced by Salmonella virginia O:8.
The antigenic O-polysaccharide of the lipopolysaccharide produced by Salmonella virginia (O:8), analyzed by methylation, partial acid hydrolysis, and one- and two-dimensional nuclear magnetic resonance methods, was found to be a polymer of a repeating pentasaccharide unit composed of D-mannose, D-galactose, L-rhamnose, D-abequose, and O-acetyl (2:1:1:1:1.3) and having the following structure: [formula; see text] The disaccharide structure alpha-D-Abep-(1----3)-L-Rhap was identified as the Salmonella O:8 antigenic factor epitope, since the removal of alpha-D-Abep residues from the O-polysaccharide left a residual tetrasaccharide repeating unit backbone that did not show reaction with Salmonella type O:8 factor antiserum.
MacLean
L L
LL
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont.
Perry
M B
MB
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005690:Galactose / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006868:Hydrolysis; D005779:Immunodiffusion; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012210:Rhamnose / Q000032:analysis; D012475:Salmonella / Q000276:immunology*
D006601:Hexoses; D008050:Lipid A; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; C023708:abequose; D008358:Mannose; D012210:Rhamnose; D005690:Galactose