title: Phosphorylation of Acinetobacter isocitrate lyase.
abstract: During growth on succinate, Acinetobacter calcoaceticus contains two forms of the enzyme isocitrate dehydrogenase. Addition of acetate to a lag-phase culture grown on succinate causes a dramatic increase in activity of form II of isocitrate dehydrogenase and in isocitrate lyase. Form II of isocitrate dehydrogenase may be responsible for the partition of isocitrate between the TCA cycle and the glyoxylate by-pass. This report describes the phosphorylation of the enzyme isocitrate lyase from A. calcoaceticus. This phosphorylation may be a regulatory mechanism for the glyoxylate by-pass.
authors.affiliation: Department of Microbiology, Arizona State University, Tempe 85287-2701.
authors.forename: J C
authors.initials: JC
authors.lastname: Hoyt
authors.forename: H C
authors.initials: HC
authors.lastname: Reeves
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000201:enzymology* / Q000254:growth & development; D001345:Autoradiography; D015153:Blotting, Western; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007522:Isocitrate Lyase / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation
chemical_list: D007527:Isoenzymes; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D007521:Isocitrate Dehydrogenase; D007522:Isocitrate Lyase