title: Changes in expression of peptides in rat facial motoneurons after facial nerve crushing and resection.
abstract: In situ hybridization histochemistry was used to study changes in mRNAs coding neuropeptides such as alpha-calcitonin gene-related peptide (CGRP), beta-CGRP, cholecystokinin (CCK) and galanin, in rat facial motoneurons following axotomy of the facial nerve. In control rats, 38%, 55% and 7% of the facial motoneurons expressed alpha-CGRP, beta-CGRP and CCK mRNAs, respectively. No galanin mRNA-containing motoneurons were observed in these animals. The levels of mRNA for alpha-CGRP, CCK and galanin were increased while the beta-CGRP mRNA level was decreased after axotomy. The levels of mRNAs for these peptides returned to the control values by 2-4 weeks after nerve crush, whereas nerve resection had more prolonged effects. Within 3-4 weeks after injury, nerve resection had greater effects on beta-CGRP, CCK and galanin mRNAs than did nerve crush. Thus, there appear to be differences in the regulation of mRNA expression of these peptides in axotomized motoneurons.
authors.affiliation: Department of Otolaryngology, Osaka University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Saika
authors.forename: E
authors.initials: E
authors.lastname: Senba
authors.forename: K
authors.initials: K
authors.lastname: Noguchi
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Kubo
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga
authors.forename: M
authors.initials: M
authors.lastname: Tohyama
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics*; D002766:Cholecystokinin / Q000235:genetics*; D005154:Facial Nerve / Q000502:physiology*; D019004:Galanin; D007700:Kinetics; D046911:Macromolecular Substances; D008297:Male; D009046:Motor Neurons / Q000166:cytology / Q000502:physiology*; D009409:Nerve Crush; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010455:Peptides / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D010455:Peptides; D012333:RNA, Messenger; D019004:Galanin; D002766:Cholecystokinin; D015740:Calcitonin Gene-Related Peptide