The main serum protease inhibitors; alpha-1-proteinase inhibitor and alpha-2-macroglobulin inhibit H2O2 release from human polymorphonuclear leukocytes stimulated with phorbol myristate acetate.
Since various functions of phagocytes can be affected by protease inhibitors, the ability of alpha-1-proteinase inhibitor (alpha 1PI) and alpha-2-macroglobulin (alpha 2M) to modulate the H2O2 production by human polymorphonuclear leukocytes (PMNL) was studied. The preincubation of PMNL for 30 min with these protease inhibitors at concentrations which may occur in human blood diminished in a dose dependent manner their H2O2 generation induced with phorbol myristate acetate. At alpha 1PI 600 mg/dl and alpha 2M 800 mg/dl the H2O2 response decreased to 61 +/- 2 and 58 + 3% (p less than 0.001, n = 4) of the control value obtained with cells preincubated in phosphate buffered saline with glucose, respectively. Autologous serum alone and with addition of pure alpha 1 PI or alpha 2M also suppressed H2O2 release from PMNL. It is suggested that inhibition of H2O2 generation from PMNL may be another additional way by which these serum protease inhibitors may protect tissues (especially lungs) from acute injury related to inflammation.
Nowak
D
D
Department of Pneumonology and Allergology, Medical Academy, Lódź.
Piasecka
G
G
D003962:Dianisidine; D004646:Emphysema / Q000209:etiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood*; D066298:In Vitro Techniques; D007249:Inflammation / Q000209:etiology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D000515:alpha 1-Antitrypsin / Q000494:pharmacology*; D000511:alpha-Macroglobulins / Q000494:pharmacology*
D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D006861:Hydrogen Peroxide; D003962:Dianisidine; D013755:Tetradecanoylphorbol Acetate