Quantitative immunohistochemical analysis of keratins and desmoplakins in human gingiva and peri-implant mucosa.
Quantitative immunohistochemistry was used to compare the distributions of keratins and desmoplakins in human gingiva and peri-implant mucosa (three specimens each). In gingiva, keratin 1 (a marker of cornification) and desmoplakins I & II (markers of desmosomes) stained most heavily in granular strata followed by corneal strata; keratin 13, a marker of non-cornifying stratified squamous cells, stained most heavily in suprabasal strata of oral sulcular epithelium. Keratin 19, a marker for junctional epithelium, stained the basal stratum of oral sulcular epithelium most heavily. In peri-implant mucosa, the patterns of staining were similar, except that staining for desmoplakins I & II was generally significantly reduced compared with gingiva, and junctional epithelium co-expressed keratins 13 and 19. Peri-implant junctional epithelial cells attached to titanium implant abutments were removed by trypsin/EDTA digestion, and also exhibited co-expression of keratins 13 and 19. Inflammatory cell infiltration was associated with reduction of keratin 1 staining in gingiva. The data indicate that the epithelia of gingiva and peri-implant mucosa are not composed of identical cell populations.
Carmichael
R P
RP
Department of Prosthodontics, Faculty of Dentistry, University of Toronto, Ontario, Canada.
McCulloch
C A
CA
Zarb
G A
GA
D000704:Analysis of Variance; D003239:Connective Tissue Cells; D003598:Cytoskeletal Proteins / Q000032:analysis*; D003758:Dental Implantation, Endosseous; D015921:Dental Implants; D051180:Desmoplakins; D003896:Desmosomes / Q000737:chemistry*; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D005881:Gingiva / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis*; D008214:Lymphocytes / Q000166:cytology; D009061:Mouth Mucosa / Q000737:chemistry* / Q000166:cytology; D013194:Staining and Labeling; D014741:Video Recording
D003598:Cytoskeletal Proteins; D015921:Dental Implants; D051180:Desmoplakins; D007633:Keratins