In vitro assays show a dissociation of reverse transcriptase activity and core antigen (p24) production in two HIV-1 isolates from a patient receiving long-term treatment with zidovudine (ZDV).
Two HIV-1 isolates were obtained from a patient receiving long-term treatment with zidovudine (ZDV). The in vitro sensitivity to ZDV triphosphate of the reverse transcriptase (RT) from both isolates appeared to be unchanged compared to that of the LAV-Bru HIV-1 reference strain. When isolates were grown in CEM cells (a T-lymphoblastoid tumor cell line) and their RT activity and core antigen (p24) production were determined, the level of p24 production compared to RT activity was high; in infected CEM cells treated with ZDV, RT activity was at background level while the p24 production was still significant, thus indicating a dissociation of RT activity and core antigen production.
Masquelier
B
B
Laboratoire de Virologie, Université de Bordeaux II, France.
Combeau
T
T
Poveda
J D
JD
Delord
B
B
Pellegrin
J L
JL
Sallafranque-Andreola
M L
ML
Tarrago-Litvak
L
L
Fleury
H J
HJ
D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D015683:Gene Products, gag / Q000096:biosynthesis*; D015488:HIV Antigens / Q000096:biosynthesis*; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D006801:Humans; D007700:Kinetics; D012367:RNA, Viral / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013698:Templates, Genetic; D014758:Viral Core Proteins / Q000096:biosynthesis*; D015215:Zidovudine / Q000627:therapeutic use*
D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D012367:RNA, Viral; D014758:Viral Core Proteins; D015215:Zidovudine; D012194:RNA-Directed DNA Polymerase