New rapid staining methods of Cryptosporidium oocysts in stools.
Two new extemporaneous negative-staining methods are proposed to detect Cryptosporidium oocysts in stools, using light-green and merbromine in 1% and 2% aqueous solution, respectively. A Ziehl-Neelsen stain as modified by Henriksen and Polhenz was used as a reference technique. A comparison between these two new stains and the reference, a modified Ziehl-Neelsen mod. method gave almost identical sensitivity and specificity. We propose their use in routine diagnosis for enteric cryptosporidiosis.
Chichino
G
G
Department of Infectious Diseases, University, IRCCS San Matteo, Pavia, Italy.
Bruno
A
A
Cevini
C
C
Atzori
C
C
Gatti
S
S
Scaglia
M
M
D000163:Acquired Immunodeficiency Syndrome / Q000469:parasitology; D000818:Animals; D003458:Cryptosporidium / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology*; D006679:HIV Seropositivity / Q000469:parasitology; D006801:Humans; D008297:Male; D008622:Merbromin; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*
D008622:Merbromin