Location of a new encephalitogenic epitope (residues 43 to 64) in proteolipid protein that induces relapsing experimental autoimmune encephalomyelitis in PL/J and (SJL x PL)F1 mice.
Synthetic peptides of proteolipid protein (PLP) were screened for their ability to induce experimental autoimmune encephalomyelitis (EAE) in SJL/J, PL/J, and (SJL x PL)F1 mice, and T cell lines were selected by stimulation of lymph node cells with PLP peptides. PLP 141-151 was found to be less encephalitogenic in SJL/J mice than PLP 139-151, due to deletion of two amino acids from the amino-terminal end. PLP 139-151 immunization induced relapsing EAE in SJL/J and F1 mice but not PL/J mice. In contrast, PLP 43-64 induced relapsing EAE in PL/J and F1 mice but not SJL/J mice. F1 T cell lines specific for either PLP 43-64 or PLP 139-151 adoptively transferred demyelinating EAE to naive F1 recipients. Haplotypes H-2s and H-2u appear to be immunologically co-dominant in F1 mice in the PLP EAE system, which differs from the H-2u dominance in F1 mice in the myelin basic protein EAE system. The identification of a PLP peptide that is encephalitogenic in PL/J mice, in addition to the previous demonstration of PLP peptides that are encephalitogenic for SWR mice (PLP 103-116) and SJL/J mice (PLP 139-151), lends support to a role for PLP as a target Ag in autoimmune demyelinating diseases.
Whitham
R H
RH
Neuroimmunology Research, Veterans Administration Medical Center, Portland, OR 97207.
Jones
R E
RE
Hashim
G A
GA
Hoy
C M
CM
Wang
R Y
RY
Vandenbark
A A
AA
Offner
H
H
D000595:Amino Acid Sequence; D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D000939:Epitopes; D007116:Immunization, Passive; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000276:immunology*; D018991:Myelin Proteolipid Protein; D010455:Peptides / Q000737:chemistry / Q000276:immunology
D000939:Epitopes; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D010455:Peptides