title: Limited proteolysis of vitronectin by plasmin destroys heparin binding activity.
abstract: Vitronectin (VN) stabilizes plasminogen activator inhibitor type 1 (PAI-1) activity and prevents the fibrin(ogen)-induced acceleration of plasminogen activation by t-PA. These antifibrinolytic activities as well as other functions are mediated by the glycosaminoglycan (GAG) binding domain of VN. Since the GAG binding region is rich in arginyl and lysyl residues, it is a potential target for enzymes such as plasmin. In this paper, the dose and time-dependent proteolysis of VN by plasmin is demonstrated. The addition of urokinase or streptokinase (200 units/ml) to plasma also produced proteolysis of VN. With minimal proteolysis, the 75 kDa band was degraded to a 62-65 kDa form of VN. This minimal proteolysis destroyed the binding of [3H]-heparin to VN and reversed the neutralization of heparin by VN. Thus, the plasmin-mediated proteolysis of the GAG binding activity of VN could destroy the antifibrinolytic activity of VN during physiologic conditions and during thrombolytic therapy. Furthermore, other functions of VN in complement and coagulation systems that are mediated by the GAG binding domain may be destroyed by plasmin proteolysis.
authors.affiliation: Department of Medicine, Duke University Medical Center, Durham, NC 27710.
authors.forename: D C
authors.initials: DC
authors.lastname: Sane
authors.forename: T L
authors.initials: TL
authors.lastname: Moser
authors.forename: C S
authors.initials: CS
authors.lastname: Greenberg
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005341:Fibrinolysin / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006493:Heparin / Q000097:blood*; D006801:Humans; D015151:Immunoblotting; D011485:Protein Binding; D011869:Radioligand Assay; D019096:Vitronectin
chemical_list: D001798:Blood Proteins; D006023:Glycoproteins; D019096:Vitronectin; D006493:Heparin; D005341:Fibrinolysin