title: Simultaneous immunohistochemical demonstration of vasoactive intestinal polypeptide and its receptor in human colon.
abstract: The present study reports an immunohistochemical approach for localizing the immunoreactivity of vasoactive intestinal polypeptide (VIP) receptor in the human colon, by using a monoclonal antibody which recognizes the VIP-receptor of a human colonic adenocarcinoma cell line. Simultaneous demonstration of immunoreactive VIP-receptor of a human colonic adenocarcinoma cell line. Simultaneous demonstration of immunoreactive VIP-receptor and VIP was achieved by a double-labelling procedure employing immunogold silver staining for VIP-receptor, and a biotinylated secondary antibody followed by streptavidin-Texas Red, to visualize VIP. The immunoreactive VIP receptor was found at two locations receiving dense VIP innervation: myenteric ganglia and mucosal epithelium. Epithelial cells displayed intense labelling at the basolateral membrane, which confirmed earlier binding studies on fractionated membranes. A small number of enteroendocrine cells was also recognized by the VIP-receptor antibody. Smooth muscle and cells of the immune system were not stained by the monoclonal antibody, indicating that it recognized an epitope not common to VIP-receptors of all locations. Thus, the immunohistochemical approach of VIP-receptor localization differs from autoradiography in (a) precise cellular localization, (b) possibility of simultaneous demonstration of receptor and ligand immunoreactivity, and (c) selectivity to a certain receptor population which, however, is presently not fully characterized.
authors.affiliation: Institute for Anatomy and Cell Biology, University of Heidelberg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Kummer
mesh_terms: D000911:Antibodies, Monoclonal; D003106:Colon / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005724:Ganglia / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards; D007413:Intestinal Mucosa / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D011964:Receptors, Gastrointestinal Hormone / Q000032:analysis*; D018005:Receptors, Vasoactive Intestinal Peptide; D013194:Staining and Labeling; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide