High density cAMP-gated channels at the ciliary membrane in the olfactory receptor cell.
Spatial distribution of the cAMP-gated channel was investigated in amphibian olfactory receptor cells. Low doses of cAMP applied to the cytoplasmic side of a membrane patch excised from cilia produced single channel activity of unitary conductance 28pS. Variance analysis showed that the ciliary membrane contained 920 cAMP gated-channels/microns2 in the newt and 2400 channels/microns2 in the toad. In contrast, the membrane of the dendrite and cell body contained only 2 cAMP-gated channels/microns2 (newt) and 6 channels/microns2 (toad). Thus, there is a high density of cAMP-gated channels in the cilia where olfactory transduction is thought to take place.
Kurahashi
T
T
Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Kaneko
A
A
D000818:Animals; D002923:Cilia / Q000378:metabolism / Q000648:ultrastructure; D000242:Cyclic AMP / Q000494:pharmacology*; D003712:Dendrites / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D015640:Ion Channel Gating; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D009297:Nasal Mucosa / Q000378:metabolism*; D009994:Osmolar Concentration; D011984:Sensory Receptor Cells / Q000378:metabolism* / Q000648:ultrastructure
D007473:Ion Channels; D000242:Cyclic AMP