Effect of anti-carbohydrate antibodies on HIV infection in a monocytic cell line (U937).
Monoclonal antibodies (mAbs) against carbohydrate epitopes of gp120 have recently been found to inhibit HIV infection of lymphocytes in vitro thereby opening new possibilities for vaccine considerations. Antibody-dependent enhancement of infection has however come increasingly into focus. This study therefore investigated the neutralization of HIV in a monocytic cell line (U937) using mAbs against these carbohydrate gp120-epitopes. While antibodies against one of the epitopes (AI) neutralized infection of U937 cells despite binding to the Fc-receptor, one mAb against the sialosyl-Tn epitope enhanced infection. This enhancement was independent of complement and could be blocked by mAb Leu3a against the CD4-receptor. The study indicated that enhancement of infection in monocytic cells can occur by the same anti-carbohydrate antibodies that neutralize infection in lymphocytes, and that antibody mediated enhancement may depend on location of the epitope on gp120 rather than whether the antibody binds Fc-receptors.
Hansen
J E
JE
Department of Infectious Diseases, Hvidovre Hospital, Denmark.
Nielsen
C
C
Clausen
H
H
Mathiesen
L R
LR
Nielsen
J O
JO
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000276:immunology*; D002460:Cell Line; D003165:Complement System Proteins / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000382:microbiology*; D011961:Receptors, Fc / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D002241:Carbohydrates; D000939:Epitopes; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120; D011961:Receptors, Fc; D003165:Complement System Proteins