title: Parathyroid hormone secretion and target organ response in experimental acute pancreatitis.
abstract: To determine changes in parathyroid hormone secretion and target organ response caused by acute pancreatitis before the development of systemic toxic conditions, experimental acute pancreatitis was induced in rats with a choline-deficient, ethionine-supplemented diet. After 7 days, the rats were weighed and bled, and one kidney was assayed for 25-hydroxyvitamin D1 hydroxylase activity. Several manifestations of pancreatitis were observed in rats given the diet: weight loss (from 29.6 to 26.3 g vs that for control rats, from 29 to 52.8 g) and lower dietary intake (15.5 vs 47 g per rat per 7 days). Serum amylase levels fell from 1794 to 350 U/L in rats given the choline-deficient, ethionine-supplemented diet compared with levels of 1800 to 2100 U/L in control rats. The pancreases of rats given the choline-deficient, ethionine-supplemented diet showed degeneration, necrosis, and hemorrhaging. Serum levels of calcium, phosphorus, chloride, and parathyroid hormone did not change significantly throughout the experiment. Renal 25-hydroxyvitamin D1 hydroxylase activity was higher than in control rats (8.9 +/- 0.8 vs 7.6 +/- 0.6 fmol/mg of kidney per minute). Acute pancreatitis in this experimental animal model does not alter serum levels of calcium and parathyroid hormone or reduce target organ responsiveness to the hormone.
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC 27710.
authors.forename: P P
authors.initials: PP
authors.lastname: Schachter
authors.forename: M D
authors.initials: MD
authors.lastname: Christy
authors.forename: B
authors.initials: B
authors.lastname: Lobaugh
authors.forename: G S
authors.initials: GS
authors.lastname: Leight
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000032:analysis*; D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D002118:Calcium / Q000097:blood; D002712:Chlorides / Q000097:blood; D002796:Choline Deficiency / Q000503:physiopathology; D005001:Ethionine / Q000494:pharmacology; D006470:Hemorrhage / Q000473:pathology; D007668:Kidney / Q000201:enzymology*; D008297:Male; D009336:Necrosis; D010195:Pancreatitis / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D010281:Parathyroid Hormone / Q000097:blood*; D010758:Phosphorus / Q000097:blood; D051381:Rats; D011916:Rats, Inbred F344; D013053:Spectrophotometry; D015431:Weight Loss
chemical_list: D002712:Chlorides; D010281:Parathyroid Hormone; D010758:Phosphorus; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; D000681:Amylases; D002118:Calcium; D005001:Ethionine