Turmerin: a water soluble antioxidant peptide from turmeric [Curcuma longa].
Dietary spice components have been screened for their protective effect against reactive oxygen species (ROS)-induced, lipid peroxide-mediated membrane and DNA damage and mutagenecity. A new, water soluble, 5-kDa peptide--Turmerin--from turmeric (Curcuma longa) has been found to be an efficient antioxidant/DNA-protectant/antimutagen. Turmerin forms 0.1% of the dry weight of turmeric and is obtained in a crystalline form. It is a heat stable, noncyclic peptide containing 40 amino acid residues, with a blocked N-terminal and leucine at the C-terminal. It is insensitive to trypsin and pepsin, heat, and uv radiation. Turmerin contains three residues of methionine which are partly responsible for the antioxidant activity. Turmerin at 183 nM offers 80% protection to membranes and DNA against oxidative injury. ROS-induced arachidonate release and the mutagenic activity of t-butyl hydroperoxide are substantially inhibited by Turmerin. Tumerin is noncytotoxic up to milligram concentrations, as tested by Ames assay and in human lymphocytes.
Srinivas
L
L
Department of Nutrition and Food Safety, Central Food Technological Research Institute, Karnataka State, India.
Shalini
V K
VK
Shylaja
M
M
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification; D001095:Arachidonic Acids / Q000378:metabolism; D002850:Chromatography, Gel; D003212:Condiments; D004220:Disulfides / Q000032:analysis; D005260:Female; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D009152:Mutagenicity Tests; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000596:Amino Acids; D016587:Antimutagenic Agents; D000975:Antioxidants; D001095:Arachidonic Acids; D004220:Disulfides; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C072268:turmerin; D013755:Tetradecanoylphorbol Acetate