Localization of a VP3 epitope of infectious bursal disease virus.
An immunodominant region of VP3, one of the two structural proteins of infectious bursal disease virus (IBDV strain 002-73), has been mapped by restriction site-specific deletion analysis and subcloning in Escherichia coli, followed by immunoblot analysis of the synthesized products. The epitope located within 58 amino acids reacted very strongly with a mouse monoclonal antibody (MAb 17/80) raised against IBDV 002-73. This immunodominant region may be useful in serodiagnosis of IBDV infection in poultry.
Jagadish
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CSIRO, Division of Biomolecular Engineering, Parkville Laboratories, Victoria, Australia.
Azad
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D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D000939:Epitopes; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics / Q000276:immunology; D015183:Restriction Mapping
D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; D011506:Proteins