The effect of hexane on the ventricular fibrillation threshold of the isolated perfused rat heart.
This investigation was conducted to determine the influence of hexane on the ventricular fibrillation threshold of the isolated perfused rat heart and myocardial electrolyte levels. Ventricular fibrillation threshold was measured using the Langendorff perfusion apparatus. Heart rate was measured by a universal digital counter and the cardiac flow by collecting the outflow of the heating chamber below the heart into a graduated measuring cylinder. Magnesium and zinc were measured by atomic absorption spectrophotometry and potassium by flame photometry. Two groups of rats were studied; those in the experimental group were given 0.2 ml of hexane and the control group 0.2 ml olive oil subcutaneously for 90 days. Their hearts were removed under anaesthesia. Half of the experimental and control hearts were mounted on the Langendorff perfusion apparatus and the heart rate, coronary flow and ventricular fibrillation threshold were measured. The hearts of the other half were used to measure myocardial electrolyte levels. In the experimental group the ventricular fibrillation threshold decreased (4.72 (S.D. +/- 1.87) vs 9.48 (S.D. +/- 2.98); P less than 0.001). There was no change in the coronary flow and heart rate in between the groups. The mean myocardial potassium levels (2586 (S.D. +/- 162) vs 2968 (S.D. +/- 218) micrograms/g; P less than 0.001), magnesium levels (164 (S.D. +/- 28) vs 208 (S.D. +/- 18) micrograms/g; P less than 0.001) and zinc levels (19.6 (S.D. +/- 4) vs 33.8 (S.D. +/- 6.8) micrograms/g; P less than 0.001) were significantly lower in the hexane-treated group compared to controls. Hexane, a constituent of glue and benzine, is cardiotoxic; marked derangement in myocardial electrolytes and a reduced ventricular fibrillation threshold, indicating an increased myocardial vulnerability to arrhythmias, was noted in the experimental animals.
Khedun
S M
SM
Department of Experimental and Clinical Pharmacology, University of Natal Medical School, Durban, South Africa.
Maharaj
B
B
Leary
W P
WP
Lockett
C J
CJ
D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D006586:Hexanes / Q000633:toxicity*; D007279:Injections, Subcutaneous; D008274:Magnesium / Q000032:analysis; D008297:Male; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000737:chemistry*; D011188:Potassium / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014693:Ventricular Fibrillation / Q000139:chemically induced* / Q000378:metabolism; D015032:Zinc / Q000032:analysis
D006586:Hexanes; D008274:Magnesium; D015032:Zinc; D011188:Potassium