Correlation of gangliotetraose gangliosides with neurite forming potential of neuroblastoma cells.
Gangliosides of 11 different neuroblastoma cell lines, grown to confluence, were extracted and quantified with respect to: (a) total lipid-bound sialic acid, (b) total gangliotetraose family, and (c) GM1 content. The cultured cells were induced to grow neurites in 3 ways: (a) serum reduction, (b) exogenous ganglioside, and (c) retinoic acid. Neurite outgrowth was quantified in terms of % of cells bearing neurites and average number of neurites per cell. No correlation was observed between neurite outgrowth and total ganglioside concentration, but a reasonably good correlation was observed with respect to neuritogenesis and gangliotetraose content. When exogenous ganglioside was the stimulant the best correlation was with GM1, whereas retinoic acid-stimulated outgrowth was approximately proportional to GD1a content. The 'neurite minus' N1A-103 line, which had the lowest level of GM1, GD1a, and total gangliotetraose gangliosides, showed little if any response to any of the stimuli.
Wu
G S
GS
Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461.
Lu
Z H
ZH
Ledeen
R W
RW
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002772:Cholera Toxin / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D005677:G(M1) Ganglioside / Q000032:analysis* / Q000302:isolation & purification; D006735:Horseradish Peroxidase; D006801:Humans; D051379:Mice; D016501:Neurites / Q000187:drug effects / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D013194:Staining and Labeling; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D005677:G(M1) Ganglioside; D014212:Tretinoin; D002772:Cholera Toxin; D006735:Horseradish Peroxidase