title: Influence of extracellular matrix on the proliferation and differentiation of glioma cells in culture.
abstract: The influence of extracellular matrix on the proliferation and differentiation of glioma cells in vitro was investigated by culturing glioma cells in collagen gel and on collagen and laminin films. Rat C-6 glioma cells extended thin cytoplasmic processes, proliferated, and differentiated in collagen gel and on collagen film. The cells were stellate with multiple thin processes like the astrocyte in vivo. The intracellular content of cyclic adenosine-monophosphate in rat C-6 glioma cells on collagen film increased approximately four fold over the control level. In laminin film culture, rat C-6 glioma cells extended thin cytoplasmic processes with many knotty structures and proliferated. These findings confirm that extracellular matrix induces the differentiation of glioma cells.
authors.affiliation: Department of Neurosurgery, Saga Medical School.
authors.forename: M
authors.initials: M
authors.lastname: Takagi
authors.forename: H
authors.initials: H
authors.lastname: Sugihara
authors.forename: K
authors.initials: K
authors.lastname: Tabuchi
mesh_terms: D000818:Animals; D001253:Astrocytes; D001812:Blood-Brain Barrier; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016543:Central Nervous System Neoplasms / Q000473:pathology*; D003094:Collagen / Q000494:pharmacology*; D003470:Culture Media; D000242:Cyclic AMP / Q000032:analysis; D005109:Extracellular Matrix / Q000502:physiology*; D005353:Fibronectins / Q000494:pharmacology*; D005909:Glioblastoma / Q000473:pathology*; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007797:Laminin / Q000494:pharmacology*; D009363:Neoplasm Proteins / Q000032:analysis; D051381:Rats; D011956:Receptors, Cell Surface / Q000502:physiology; D038981:Receptors, Collagen; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology
chemical_list: D003470:Culture Media; D005353:Fibronectins; D007797:Laminin; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; D003994:Bucladesine; D003094:Collagen; D000242:Cyclic AMP