Study on the interaction of aromatic dyes with nucleic acids by means of UV, CD and NMR spectroscopies.
The interaction of several aromatic cationic dyes such as, ethidium bromide (EB), methylene blue (MB), acridine orange (AO), and Hoechst 33258 with calf-thymus DNA and poly(A)-poly(U) duplex was investigated. The different induced extrinsic Cotton effects (greater than 300 nm) were observed for DNA- and RNA-dye complexes. The binding properties of these complexes were examined by UV, CD, and NMR spectroscopies.
Yashima
E
E
Department of Applied Chemistry, Faculty of Engineering, Kagoshima University, Japan.
Tajima
T
T
Suehiro
N
N
Akashi
M
M
Miyauchi
N
N
D000165:Acridine Orange / Q000378:metabolism; D000818:Animals; D006690:Bisbenzimidazole / Q000378:metabolism; D002417:Cattle; D002942:Circular Dichroism; D004396:Coloring Agents / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004996:Ethidium / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008751:Methylene Blue / Q000378:metabolism; D011063:Poly A-U / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D004396:Coloring Agents; D011063:Poly A-U; D004247:DNA; D004996:Ethidium; D000165:Acridine Orange; D006690:Bisbenzimidazole; D008751:Methylene Blue