Bacterial metabolism of 3-chloroacrylic acid.
Two bacterial strains were isolated with 3-chloroacrylic acid (CAA) as sole source of carbon and energy. Strain CAA1, a Pseudomonas cepacia sp., was capable of growth with only the cis-isomer of CAA. Strain CAA2, a coryneform bacterium, utilized both isomers of CAA as sole source of carbon and energy. Strain CAA1 contained cis-CAA hydratase and strain CAA2 contained two hydratases, one with cis-CAA hydratase activity and one with trans-CAA hydratase activity. The product of the hydratase activities with CAA was malonate semialdehyde. In both strains malonate semialdehyde was subsequently decarboxylated by a cofactor-independent decarboxylase yielding acetaldehyde and CO2.
Hartmans
S
S
Division of Industrial Microbiology, Agricultural University, Wageningen, The Netherlands.
Jansen
M W
MW
van der Werf
M J
MJ
de Bont
J A
JA
D000079:Acetaldehyde / Q000378:metabolism; D000179:Acrylates / Q000378:metabolism*; D000192:Actinomycetales / Q000201:enzymology / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D001673:Biodegradation, Environmental; D016956:Burkholderia cepacia / Q000201:enzymology / Q000378:metabolism*; D002248:Carbon Monoxide / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D005649:Fumarate Hydratase / Q000378:metabolism; D006835:Hydrazones / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007522:Isocitrate Lyase / Q000378:metabolism; D008314:Malonates / Q000378:metabolism; D008315:Malondialdehyde / Q000031:analogs & derivatives / Q000378:metabolism; D013058:Mass Spectrometry
C001072:2,4-dinitrophenylhydrazone; D000179:Acrylates; D002712:Chlorides; D006835:Hydrazones; D008314:Malonates; D008315:Malondialdehyde; C071773:3-chloroacrylic acid; D002248:Carbon Monoxide; C039786:malonic semialdehyde; D007521:Isocitrate Dehydrogenase; D000445:Aldehyde Oxidoreductases; C018699:aldehyde dehydrogenase (NAD(P)+); D002262:Carboxy-Lyases; C071852:malonate decarboxylase; C071850:malonate semialdehyde decarboxylase; D007522:Isocitrate Lyase; C071851:3-chloroacrylic acid hydratase; D006836:Hydro-Lyases; D005649:Fumarate Hydratase; D000079:Acetaldehyde