title: Allergenic fragments in Parietaria judaica pollen extract.
abstract: High-performance ion-exchange chromatography and immunoaffinity chromatography suggest that Par jI, the principal allergenic component of Parietaria judaica pollen, is a very unstable molecule, which tends to fragment in solution. Several fragments were obtained from Par jI and some of them show positivity toward the anti-Par jI monoclonal antibody, suggesting that they retain the entire structure of the allergenic determinant. These fragments could be the target for sequence and conformation studies.
authors.affiliation: Dipartimento di Chimica Organica e Industriale, Università di Milano, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Bolzacchini
authors.forename: P
authors.initials: P
authors.lastname: Di Gennaro
authors.forename: G
authors.initials: G
authors.lastname: Di Gregorio
authors.forename: B
authors.initials: B
authors.lastname: Rindone
authors.forename: P
authors.initials: P
authors.lastname: Falagiani
authors.forename: G
authors.initials: G
authors.lastname: Mistrello
authors.forename: I
authors.initials: I
authors.lastname: Sondergaard
mesh_terms: D000485:Allergens / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D000939:Epitopes / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007158:Immunologic Techniques; D011058:Pollen / Q000032:analysis* / Q000276:immunology; D011852:Radioallergosorbent Test
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D000939:Epitopes