title: Developmental expression of the 25-kDa synaptosomal-associated protein (SNAP-25) in rat brain.
abstract: The developmental expression and subcellular distribution of the neuron-specific 25-kDa synaptosomal protein (SNAP-25) were investigated by using Northern (RNA) blots, immunoblots, and immunocytochemistry. Both SNAP-25 protein and mRNA were present at low levels in embryonic day 15 rat brain, and levels of both increased during early postnatal maturation. Developmental immunoblots with antipeptide antisera demonstrated that a 25-kDa peptide was the major isoform in brain, and this form increased steadily from embryonic day 15 through adulthood. A second 27-kDa immunoreactive isoform was present in brain only during early development. Immunoblots of two-dimensional SDS/polyacrylamide gels revealed the presence of a predominant 25-kDa isoform of SNAP-25 in adult brain. Immunocytochemical studies indicated that as immunoreactivity for SNAP-25 increased during development, the cellular localization of SNAP-25 immunoreactivity concomitantly shifted from axons and cell bodies to presynaptic terminals. These data suggest that the SNAP-25 protein shifts in subcellular localization during development and may play a role in the establishment and stabilization of specific presynaptic terminals in brain.
authors.affiliation: Department of Pharmacology Pennsylvania State University College of Medicine, Hershey 17033.
authors.forename: G A
authors.initials: GA
authors.lastname: Oyler
authors.forename: J W
authors.initials: JW
authors.lastname: Polli
authors.forename: M C
authors.initials: MC
authors.lastname: Wilson
authors.forename: M L
authors.initials: ML
authors.lastname: Billingsley
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D007150:Immunohistochemistry; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012313:RNA / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D050825:Synaptosomal-Associated Protein 25
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25; D012313:RNA