Monoclonal anti-La antibody derived from a mouse with experimental SLE is similar to human anti-La antibodies.
The La antigen is a highly conserved protein, originally defined by sera of patients with Sjögren's syndrome or systemic lupus erythematosus (SLE). In the present study, we have produced and characterized a monoclonal anti-la antibody derived from mice with experimental SLE. The induction of SLE in these mice was achieved by their immunization with a murine monoclonal anti-idiotypic antibody against a common idiotype (16/6 Id) found in SLE patients. The monoclonal anti-La antibody derived from these mice was found to be virtually identical to the anti-La antibodies found in human autoimmune sera. First, its binding to different nuclear extracts, as well as to protease-digested HeLa nuclear protein extracts, was found to be identical to that of human anti-La antibodies. Second, an inhibition study on blotted proteins demonstrated a very close relationship between the epitopes recognized by the murine monoclonal anti-La antibody and the human anti-La serum. Third, the monoclonal anti-La antibody was found by immunofluorescence to be directed against a nuclear antigen that gave a speckled pattern. Finally, the monoclonal anti-La antibody immunoprecipitated the La-associated small RNAs. This report provides evidence for the similarity of murine anti-La antibody produced in experimental SLE and human anti-La antibodies formed in autoimmunity.
Offen
D
D
Department of Organic Chemistry, Weizmann Institute of Science, Rehovot, Israel.
Mendlovic
S
S
Fricke
H
H
Sperling
R
R
Sperling
J
J
Mozes
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D001667:Binding, Competitive; D000939:Epitopes; D006367:HeLa Cells / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008970:Molecular Weight; D011233:Precipitin Tests; D012261:Ribonucleoproteins; D000097585:SS-B Antigen
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D000939:Epitopes; D012261:Ribonucleoproteins; D000097585:SS-B Antigen