title: The effect of PGE1 and temperature on lung function following preservation.
abstract: We studied the effect of a vasodilator (prostaglandin E1) as well as flush (F) and storage (S) temperatures (4 degrees C or 10 degrees C) on lung preservation in an isolated rabbit lung perfusion model. Low-potassium dextran (LPD) or Euro-Collins (E-C) solution was used as flush solution. Six groups of six animals were studied: group 1 (LPD, 4 degrees C F-S), group 2 (LPD with PGE1, 4 degrees C F-S), group 3 (E-C with PGE1, 4 degrees C F-S), group 4 (LPD, 10 degrees C F-S), group 5 (LPD with PGE1, 10 degrees C F-S), group 6 (E-C with PGE1, 10 degrees C F-S). After 18-hr preservation, left lungs alone were ventilated, and reperfused with fresh venous blood. PaO2, PaCO2, pulmonary artery pressure (PAP), tracheal pressure (Pt) during reperfusion, and wet/dry weight (W/D) ratios were measured. PaO2 after LPD with or without PGE1 was significantly higher than after E-C with PGE1 at 4 degrees C (95.8 +/- 11.5 mmHg in group 1 or 102.7 +/- 8.6 in group 2 vs. 41.8 +/- 10.5 in group 3, P less than 0.01) and at 10 degrees C (119.3 +/- 2.3 in group 4 or 131.1 +/- 6.2 in group 5 vs. 54.6 +/- 5.2 in group 6, P less than 0.01). PaCO2, PAP, Pt, and W/D ratios in the LPD groups were lower than in the E-C groups. LPD/PGE1 and LPD alone produced similar pulmonary preservation. PaO2 of lungs flushed with LPD and preserved at 10 degrees C was higher than that of lungs stored at 4 degrees C. We conclude that LPD solution is superior to E-C solution in this ex vivo rabbit lung preservation model, even when PGE1 is used. A moderate dose of PGE1 did not improve the performance of LPD as a flush solution. Pulmonary preservation with LPD at 10 degrees C is superior to preservation at 4 degrees C.
authors.affiliation: Department of Surgery, University of Toronto, Toronto General Hospital, Ontario, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Ueno
authors.forename: H
authors.initials: H
authors.lastname: Yokomise
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: J
authors.initials: J
authors.lastname: Puskas
authors.forename: E
authors.initials: E
authors.lastname: Mayer
authors.forename: A S
authors.initials: AS
authors.lastname: Slutsky
authors.forename: G A
authors.initials: GA
authors.lastname: Patterson
mesh_terms: D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D003080:Cold Temperature; D003911:Dextrans / Q000494:pharmacology; D006982:Hypertonic Solutions / Q000494:pharmacology; D008168:Lung / Q000502:physiology; D016040:Lung Transplantation / Q000379:methods / Q000502:physiology*; D009926:Organ Preservation / Q000379:methods*; D010100:Oxygen / Q000502:physiology; D010313:Partial Pressure; D011188:Potassium / Q000494:pharmacology; D011651:Pulmonary Artery / Q000502:physiology; D011817:Rabbits; D013696:Temperature
chemical_list: D003911:Dextrans; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D000527:Alprostadil; D011188:Potassium; D010100:Oxygen