Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors.
We describe a nonviral transformation strategy for the establishment of permanent cell lines derived from the progeny of individual mouse splenic macrophage (M phi) progenitors. These colony stimulating factor-1 (CSF-1)-dependent cell lines possess many features of mature M phi s, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor. It was also possible to immortalize selected clones of splenic M phi s differing in their constitutive antigen-presenting activities with the retention of the antigen-presenting phenotype in the resultant cell lines. The approach described in this report should be useful in obtaining additional cell lines of M phi s expressing other phenotypes of interest.
Wilson
C M
CM
Department of Immunology, St. Jude Children's Research Hospital Memphis, TN 38101-0318.
Gatewood
J W
JW
McCormack
J M
JM
Walker
W S
WS
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009113:Muramidase / Q000378:metabolism; D010641:Phenotype; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000032:analysis; D013154:Spleen / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*
D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor; D012194:RNA-Directed DNA Polymerase; D009113:Muramidase