[Photometric detection of erythrocyte phagocytosis in the monocyte-macrophage assay].
The relative rate of red blood cell (RBC) in vitro phagocytosis by monocytes was measured photometrically by the diaminobenzidinehemoglobin reaction. Our results demonstrate, that the in vitro monocyte-macrophage assay (MMA) allows a specific and sensitive estimation of low RBC-phagocytosis rates. Therefore, the MMA is a useful tool for further characterisation of antibody-monocyte interaction and is of clinical importance in blood group antibody analysis.
Sibroswki
W
W
Abt. f. Transfusionsmedizin u. Transplantationsimmunologie, Universitätsklinikum Eppendorf, Hamburg.
Cassens
U
U
Kühnl
P
P
D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D010783:Photometry
D007518:Isoantibodies
Photometrischer Nachweis der Erythrozyten-Phagozytose im Monozyten-Makrophagen-Assay (MMA).