Histamine and histidine decarboxylase are correlated with mucosal repair in rat small intestine after ischemia-reperfusion.
The aim of this experiment was to demonstrate whether histamine and histidine decarboxylase (HDC) contribute to mucosal repair in small intestine subjected to ischemia-reperfusion (I/R). The superior mesenteric artery was occluded for 15 min followed by reperfusion. In jejunal mucosa, histamine content and HDC activity increased after I/R. Histamine output in mesenteric lymph was also elevated after I/R. These increases in HDC activity, and mucosal and lymph histamine levels were suppressed by pretreatment of alpha-fluoromethylhistidine (alpha-FMH), a suicide inhibitor of HDC. alpha-FMH also attenuated the increase of ornithine decarboxylase (ODC) activity normally observed after I/R. Transport of dietary lipid into lymph markedly decreased at 24 h after I/R, yet it was restored to normal at 48 h after I/R. alpha-FMH inhibitor led to a sustained deficit in lipid transport at 48 h after I/R. This sustained functional impairment in alpha-FMH treated animals was associated with blunted responses of HDC activity and histamine content to I/R. Our results suggest that histamine and HDC contribute to the restoration in mucosal function observed at 48 h after I/R. This response may be related, at least in part, to stimulation of ODC activity by histamine.
Fujimoto
K
K
Department of Physiology, Louisiana State University Medical Center, Shreveport 71130.
Imamura
I
I
Granger
D N
DN
Wada
H
H
Sakata
T
T
Tso
P
P
D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D000818:Animals; D004041:Dietary Fats / Q000378:metabolism; D006632:Histamine / Q000378:metabolism*; D006640:Histidine Decarboxylase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D007583:Jejunum / Q000378:metabolism / Q000473:pathology; D008221:Lymphoid Tissue / Q000378:metabolism; D008297:Male; D008762:Methylhistidines / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000378:metabolism; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D012697:Serine Endopeptidases / Q000378:metabolism
D004041:Dietary Fats; D008762:Methylhistidines; C028309:alpha-fluoromethylhistidine; D006632:Histamine; D006631:Amine Oxidase (Copper-Containing); D012697:Serine Endopeptidases; C057818:cytotoxic T lymphocyte-specific serine protease; D009955:Ornithine Decarboxylase; D006640:Histidine Decarboxylase