Effect of NCDC, a protease inhibitor, on histamine release from rat peritoneal mast cells induced by anti-IgE.
NCDC dose-dependently inhibited histamine release from rat peritoneal mast cells induced by anti-IgE. Moreover, NCDC inhibited Ca(2+)-mobilization from intracellular Ca(2+)-stores as well as histamine release in mast cells activated by anti IgE, the effect on both of these phenomena being closely correlated. Anti-IgE induced a rapid increase in IP3 production from phosphoinositides in mast cells, with its production in 15 sec, followed to baseline levels within 1 min. Anti-IgE stimulated PLC activity on mast cells membrane preparation. NCDC dose-dependently inhibited the generation of IP3. These results suggest that the inhibitory effect of NCDC on the release of histamine induced by anti-IgE is due to, in part at least, the inhibition of PI-specific PLC and that the inhibitory effects of NCDC are involved in intracellular calcium store.
Takei
M
M
Department of Pharmacology, Tokushima Bunri University, Japan.
Ueno
M
M
Endo
K
K
Nakagawa
H
H
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D002118:Calcium / Q000378:metabolism; D002219:Carbamates / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects*; D007073:Immunoglobulin E / Q000276:immunology*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D048448:Phenylcarbamates; D011480:Protease Inhibitors / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D000888:Antibodies, Anti-Idiotypic; D002219:Carbamates; D048448:Phenylcarbamates; D011480:Protease Inhibitors; C025508:2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate; D007073:Immunoglobulin E; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium