[Demonstration of the differential expression of IGF-II in various embryonal kidney tumors using slot-blot hybridization and in-situ hybridization].
IGF-II may play a decisive role in the development of Wilms tumors since an elevated expression of this important embryonal growth factor has been reported in the majority of nephroblastomas. In our series of 15 typical triphasic or blastemal predominant nephroblastomas slot-blot-hybridization revealed a marked increase of IFG-II-mRNA in the tumor tissue of 11 patients. Compared to normal kidney tissue IGF-II-expression was elevated up to 64 times. Apart from nephroblastoma a number of other embryonal renal tumors with either a much better or much worse prognosis was investigated. A moderately increased expression of IGF-II was noted in 2 congenital mesoblastic nephromas. IGF-II-mRNA was only slightly increased in a clear cell sarcoma of the kidney but was not elevated in 2 malignant rhabdoid tumors of the kidney. In-situ-hybridization allowed precise localization of the markedly increased IGF-II mRNA to blastemal cells. Differentiation to epithelial structures such as tubules or glomeruli or to stromal cells was associated with a marked loss in IGF-II expression.
Briner
J
J
Institut für Pathologie, Universität Zürich.
Hassam
S
S
Rohrer
R
R
D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D007680:Kidney Neoplasms / Q000235:genetics / Q000473:pathology*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012208:Rhabdomyosarcoma / Q000235:genetics / Q000473:pathology*; D009396:Wilms Tumor / Q000235:genetics / Q000473:pathology*
D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II
Nachweis der unterschiedlichen Expression von IGF-II bei verschiedenen embryonalen Nierentumoren durch slot-blot-Hybridisierung und in-situ-Hybridisierung.