Interferon induction by cell-cell interaction of HIV-infected monocytes/macrophages with lymphocytes.
An in vitro model of interferon (IFN) induction in HIV infection was established. IFN are induced by a cooperative mechanism, involving monocytes/macrophages (M/M) as well as lymphocytes. M/M non-lytically infected with HTLV-IIIB did not produce IFN, but were able to induce high titres of IFN activity when cocultured with peripheral blood lymphocytes, cell-cell contact being required. IFN alpha as well as IFN gamma were induced, as shown by neutralization with specific antisera. The antiviral activity of HIV-induced IFN was shown by infectivity reduction assays, and the immune-modulating capacity was demonstrated by activation of M/M leading to neopterin release.
Rokos
K
K
AIDS-Zentrum des Bundesgesundheitsamtes, Robert Koch-Institut, Berlin.
Pauli
G
G
D001708:Biopterins / Q000031:analogs & derivatives / Q000378:metabolism; D002450:Cell Communication; D002478:Cells, Cultured; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007372:Interferons / Q000378:metabolism*; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D019798:Neopterin; D014779:Virus Replication
D001708:Biopterins; D019798:Neopterin; D007372:Interferons