title: Bleomycin-iron complexes and DNA radiation damage.
abstract: Radiation in the form of high-energy electrons dose-dependently activates bleomycin-Fe3+ in oxygen-containing DNA solutions. This activation causes a DNA fragmentation and a release of oxidative degradation products from the DNA. During irradiation, bleomycin-chelated Fe3+ is reduced to Fe2+. The kinetics of DNA base propenal-formation (measured by reaction with thiobarbituric acid) and iron-reduction (measured by bathophenanthroline chelation) are similar, with a yield of 1 mol base propenal/6 mol Fe3+ reduced. The activation of bleomycin-Fe3+ by irradiation could be instrumental in the synergistic action of radiotherapy and bleomycin observed on simultaneous administration in vivo.
authors.affiliation: Danish Cancer Society, Department of Virus and Cancer, Arhus.
authors.forename: N
authors.initials: N
authors.lastname: Nørskov-Lauritsen
authors.forename: P
authors.initials: P
authors.lastname: Ebbesen
authors.forename: E J
authors.initials: EJ
authors.lastname: Demant
authors.forename: J M
authors.initials: JM
authors.lastname: Christensen
mesh_terms: D001761:Bleomycin / Q000737:chemistry / Q000528:radiation effects* / Q000031:analogs & derivatives; D004247:DNA / Q000737:chemistry / Q000528:radiation effects*; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D004492:Edetic Acid; D004587:Electrophoresis, Agar Gel; D010084:Oxidation-Reduction
chemical_list: C023503:iron bleomycin; D001761:Bleomycin; D004247:DNA; D004492:Edetic Acid