title: T-cell translocation gene 1 (Ttg-1) encodes a nuclear protein normally expressed in neural lineage cells.
abstract: We previously identified and cloned T-cell translocation gene 1 (Ttg-1), a putative zinc finger protein, as a result of its deregulated expression in a T-cell acute lymphoblastic leukemia cell line (RPMI 8402) with a t(11;14)(p15;q11). We have now characterized its genomic organization and identified the major transcriptional start site to lie within an initiator-like motif. Ttg-1 is normally expressed in mouse brain and not in thymus. The mouse neuroblastoma cell line, N2a, also expresses Ttg-1. Antibodies raised against a TrpE-Ttg-1 fusion protein precipitate an 18-Kd nuclear protein from metabolically labeled 8402 cells. Immunofluorescence of N2a cells shows a nuclear pattern. The two potential zinc finger domains in Ttg-1 are highly homologous to similar regions in lin-11, mec-3, and lsl-1. This data suggests that Ttg-1 may be involved in gene regulation.
authors.affiliation: Department of Medicine, Howard Hughes Medical Institute, Washington University, St Louis, MO.
authors.forename: E A
authors.initials: EA
authors.lastname: McGuire
authors.forename: A R
authors.initials: AR
authors.lastname: Davis
authors.forename: S J
authors.initials: SJ
authors.lastname: Korsmeyer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005809:Genes, Regulator / Q000235:genetics*; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D009687:Nuclear Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011233:Precipitin Tests; D012313:RNA / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics; D014178:Translocation, Genetic / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D000912:Antibodies, Neoplasm; D009687:Nuclear Proteins; D012313:RNA