[The inhibition by strongly acidic polypeptides of the repair of double-stranded DNA breaks induced by the gamma irradiation of Chinese hamster fibroblast cells].
The ability of polypeptides consisted of aspartic and glutamic acids to inhibit the repair and to promote the formation of unrepaired double-strand DNA breaks and chromosomal aberrations in gamma-ray induced Chinese hamster cells was shown. A complete inhibition of the double-strand DNA breaks repair was observed at the concentrations of 20 mu M/l (polyglutamic acid with molecular weight 2000-15,000 daltons) and 100 mu M/l (aspartylglutamic acid with molecular weight 1500-4500 daltons). Both polypeptides were low toxic at the given concentrations.
Medvedev
A I
AI
Revina
G I
GI
Kuzin
A M
AM
D000818:Animals; D002999:Clone Cells / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects*; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects*; D003864:Depression, Chemical; D004151:Dipeptides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010455:Peptides / Q000494:pharmacology*; D011099:Polyglutamic Acid / Q000494:pharmacology
D004151:Dipeptides; D010455:Peptides; D011099:Polyglutamic Acid; C038676:aspartylglutamate; D004247:DNA
Ingibirovanie sil'nokislymi polipeptidami reparatsii dvunitevykh razryvov DNK, indutsirovannykh gamma-oblucheniem kletok fibroblastov kitaĭskogo khomiachka.