On the spontaneous adherence of myelin basic protein to T lymphocytes.
We have applied a double tagging system in order to study whether purified myelin basic protein is able to adhere to normal human peripheral T lymphocytes without the need to purify cells. Evaluation of myelin basic protein adherence to peripheral blood mononuclear cells was determined with biotinylated myelin basic protein and fluoresceinated avidin, and lymphocyte population was identified by the corresponding phycoerythrinated monoclonal antibody. The observed adherence of myelin basic protein to T lymphocytes was found to depend on protein conformation.
Bobba
A
A
Centro Studio sui Mitocondri e Metabolismo Energetico, CNR, Università di Bari, Italy.
Munno
I
I
Greco
B
B
Pellegrino
N M
NM
Riccio
P
P
Jirillo
E
E
Quagliariello
E
E
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D002417:Cattle; D002478:Cells, Cultured; D006801:Humans; D004676:Myelin Basic Protein / Q000378:metabolism*; D011485:Protein Binding; D016176:T-Lymphocyte Subsets / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
D015703:Antigens, CD; D004676:Myelin Basic Protein