title: Potentiation of bleomycin cytotoxicity by high molecular weight polyacrylic acid. II. Involvement of rapid conformational change of polyacrylate.
abstract: We report that bleomycin (BLM) cytotoxicity is dramatically potentiated in the presence of high molecular weight polyacrylic acid (A119). For potentiation, an appropriate amount of divalent cations such as Ca++, Mg++, or Ba++ was required, in addition to vortex - stirring of the reaction mixture. There was no potentiation when A119 alone was pre-stirred or left standing for several days in the presence of divalent cations prior to use. Taking into account of kinematic viscosity of A119 observed during the treatment, a rapid conformational change of A119 in the presence of divalent cations might be involved in the potentiation mechanism.
authors.affiliation: Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Sarai
authors.forename: K
authors.initials: K
authors.lastname: Kohda
authors.forename: Y
authors.initials: Y
authors.lastname: Kawazoe
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D004357:Drug Synergism; D007700:Kinetics; D007939:Leukemia L1210; D051379:Mice; D008968:Molecular Conformation; D014783:Viscosity
chemical_list: D000180:Acrylic Resins; D000970:Antineoplastic Agents; D002413:Cations, Divalent; D001761:Bleomycin; C006903:carbopol 940; D002118:Calcium