Structural and immunochemical studies of O-specific polysaccharide of Proteus vulgaris 5/43 belonging to OX19 group (O-variants).
O-specific polysaccharide was obtained on mild acid degradation of lipopolysaccharide of Proteus vulgaris 5/43 belonging to OX19 (O-variants). It was found to contain D-glucose, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2,6-dideoxy-L-glucose (QuiNAc, N-acetyl-L-quinovosamine) in the ratio of about 1:2:1, the last-named sugar being rather uncommon for bacterial antigens. A computer-assisted 13C-NMR-based analysis, methylation analysis, and selective cleavage with anhydrous hydrogen fluoride and dilute hydrochloric acid were applied for structural elucidation of the polysaccharide and the following structure was established:----2)-beta-D-Glcp-(1----6)-alpha-D-GlcpNAc-(1----3)- alpha-L-QuipNAc-(1----3)-beta-D-GlcpNAc-(1----. Serological studies of the O-antigen and oligosaccharides derived therefrom revealed the importance of the trisaccharide fragment beta-D-Glcp-(1----6)-alpha-D-GlcpNAc-(1----3)-alpha-L- QuipNAc in manifesting the antigenic specificity. Serological cross-reactions were demonstrated between lipopolysaccharides of P. vulgaris 5/43, 8/44, and OX19 strains.
Vinogradov
E V
EV
N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow.
Kaca
W
W
Rózalski
A
A
Shashkov
A S
AS
Cedzynski
M
M
Knirel
Y A
YA
Kochetkov
N K
NK
D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000737:chemistry; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology; D002240:Carbohydrate Sequence; D003429:Cross Reactions / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005947:Glucose / Q000737:chemistry; D006858:Hydrofluoric Acid / Q000494:pharmacology; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology; D011514:Proteus vulgaris; D011817:Rabbits
D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; C070625:2-acetamido-2,6-dideoxyglucose; D005947:Glucose; D006858:Hydrofluoric Acid; D000117:Acetylglucosamine