title: Oligonucleotides and their derivatives as tools for investigations of protein-nucleic acid interactions in template biocatalysis.
abstract: On the basis of quantitative characteristics (Kd, Km, Gibbs energy values) for the interaction of oligonucleotides with template and primer site of eucaryotic and procaryotic DNA polymerases, a general model of template-primer interaction with these enzymes was suggested. The interactions of AMV- and HIV- reverse transcriptases with various 5'-derived oligonucleotides and with human DNA polymerase alpha and Klenow fragment are compared. The results obtained suggest a method to improve selectively the affinity of an oligonucleotide primer to RNA template with AMV- and HIV-reverse transcriptases.
authors.affiliation: Institute of Bioorganic Chemistry, Siberian Division, Academy of Sciences of USSR, Novosibirsk.
authors.forename: O I
authors.initials: OI
authors.lastname: Lavrik
mesh_terms: D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001482:Base Composition; D001483:Base Sequence; D002384:Catalysis; D004256:DNA Polymerase I / Q000378:metabolism*; D006678:HIV / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009696:Nucleic Acids / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides; D011506:Proteins / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D013698:Templates, Genetic; D013816:Thermodynamics
chemical_list: D009696:Nucleic Acids; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I