title: Gold blot for detection of immunoglobulin M (IgM)- and IgG-specific antibodies for rapid serodiagnosis of melioidosis.
abstract: Gold blot tests for rapid serodiagnosis of melioidosis were developed and evaluated with sera from 40 melioidosis patients and 159 normal controls. The sensitivity and specificity were 87.5 and 88%, respectively, for the immunoglobulin M (IgM) test and 100 and 91%, respectively, for the protein A test for IgG. Combination of the IgM gold blot and protein A gold blot yielded 97.5% sensitivity and 94.3% specificity. The tests were rapid and simple.
authors.affiliation: Department of Pathology, Faculty of Medicine-Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: M
authors.initials: M
authors.lastname: Kunakorn
authors.forename: B
authors.initials: B
authors.lastname: Petchclai
authors.forename: K
authors.initials: K
authors.lastname: Khupulsup
authors.forename: P
authors.initials: P
authors.lastname: Naigowit
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D016957:Burkholderia pseudomallei / Q000276:immunology*; D002648:Child; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D015151:Immunoblotting / Q000379:methods* / Q000706:statistics & numerical data; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D008554:Melioidosis / Q000175:diagnosis* / Q000276:immunology; D008875:Middle Aged; D012698:Serologic Tests; D013205:Staphylococcal Protein A
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A