Islet amyloid polypeptide has no effect on amylase release from rat pancreatic acini stimulated by CCK-8, secretin, carbachol and VIP.
Islet amyloid polypeptide (IAPP), a novel peptide recently isolated from the amyloid substance of pancreatic islets, has been localized in normal islet B cell granules, suggesting its physiological action in exocrine pancreatic function. In the present study, the direct effect of IAPP on secretagogue-stimulated pancreatic enzyme secretion was investigated using dispersed rat pancreatic acini. Various concentrations of IAPP (10(-5) -10(-10) M) showed no significant influence on amylase release stimulated either by submaximal doses of CCK-8 (10(-11) M), secretin (0.5 x 10(-6) M) or carbachol (10(-6) M), or by a maximal dose of vasoactive intestinal peptide (10(-8) M). These results may provide negative evidence for a physiological significance of IAPP in rat pancreatic enzyme secretion.
Kikuchi
Y
Y
Third Department of Internal Medicine, Tohoku University School of Medicine, Sendai.
Koizumi
M
M
Shimosegawa
T
T
Kashimura
J
J
Suzuki
S
S
Toyota
T
T
D000681:Amylases / Q000378:metabolism*; D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D066298:In Vitro Techniques; D058228:Islet Amyloid Polypeptide; D008297:Male; D010179:Pancreas / Q000187:drug effects* / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D012633:Secretin / Q000494:pharmacology; D012844:Sincalide / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D012633:Secretin; D014660:Vasoactive Intestinal Peptide; D002217:Carbachol; D000681:Amylases; D012844:Sincalide