Bleomycin resistance as a selectable marker for transformation of the eukaryote, Dictyostelium discoideum.
An expression cassette was constructed, which has the bacterial bleomycin (Bm) resistance-encoding gene (ble) fused to the Dictyostelium discoideum actin-6 promoter, with a segment of 3'-flanking DNA from the actin-8-encoding gene placed downstream from the ble gene to serve as a transcription terminator. Plasmid pMUW161, which contains this cassette and the D. discoideum plasmid Ddp2 origin of DNA replication, transformed D. discoideum with high efficiency under Bm selection. Hence, this construct is useful as a dominant selectable marker for D. discoideum.
Chang
A C
AC
School of Biological Sciences, Macquarie University, Sydney, N.S.W., Australia.
Hall
R M
RM
Williams
K L
KL
D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001761:Bleomycin / Q000494:pharmacology*; D004261:DNA Replication / Q000235:genetics; D004023:Dictyostelium / Q000187:drug effects / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010692:Phleomycins / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D014170:Transformation, Genetic / Q000235:genetics*
D000199:Actins; D005819:Genetic Markers; D010692:Phleomycins; D001761:Bleomycin