Co-purification of a small RNA species with multicatalytic proteinase (proteasome) from rat liver.
Previous studies have come to different conclusions about the presence of RNA in particles known variously as prosomes, proteasomes or multicatalytic proteinase (MCP). To determine the reason for this, MCP was isolated from rat liver by 4 different purification protocols. One major band of RNA, about 80 nucleotides in length, co-purified in all preparations. The amount of RNA detected was less than one molecule per MCP particle suggesting that there may be more than one population of MCP in rat liver cells.
Skilton
H E
HE
Department of Biochemistry, University of Leicester, UK.
Eperon
I C
IC
Rivett
A J
AJ
D000818:Animals; D003546:Cysteine Endopeptidases / Q000737:chemistry*; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry*; D046988:Proteasome Endopeptidase Complex; D012313:RNA / Q000032:analysis*; D051381:Rats; D012261:Ribonucleoproteins / Q000737:chemistry
D009097:Multienzyme Complexes; D012261:Ribonucleoproteins; D012313:RNA; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex