title: Interleukin-3-dependent hematopoietic stem cell lines capable of osteoclast formation in vitro.
abstract: Recently we reported that the osteoclast originates from the pluripotent hematopoietic stem cell. However, a detailed analysis of the progenitor and precursor stages of the osteoclast lineage is hard to perform with primary cultures of stem cells. In the present investigation interleukin-3 (IL-3)-dependent multipotent hematopoietic stem cell lines (FDCP-mix), which have many characteristics in common with freshly isolated hematopoietic stem cell lines (FDCP-mix), which have many characteristics in common with freshly isolated hematopoietic stem cells, were assayed for their osteoclast formation capacity. FDCP-mix cell lines A4, C2GM, and 15S were cocultured with periosteum-free 17-day-old fetal metatarsal bones. The effects of culture time, medium composition, and addition of WEHI-3b-conditioned medium (an unpurified IL-3 preparation) on osteoclast formation were studied. 15S cells never differentiated into osteoclasts. Both A4 and C2GM cells were able to generate osteoclasts. Osteoclast formation was visualized by staining for tartrate-resistant acid phosphatase activity and confirmed by 45Ca release assays and electron microscopic studies. Medium supplemented with fetal calf serum clearly supported osteoclast formation from A4 cells better than medium supplemented with cock serum. The difference between fetal calf serum and horse serum is generally less pronounced. C2GM cells formed osteoclasts more readily and, generally, earlier than A4 under all culture conditions. WEHI-3b-conditioned medium addition increased the numbers of osteoclasts and their resorption activity. The coculture of stripped metatarsal bones with FDCP-mix cell lines therefore offers a model system with many possibilities for the study of osteoclastogenesis and its regulation.
authors.affiliation: Laboratory of Cell Biology and Histology, Leiden, The Netherlands.
authors.forename: C E
authors.initials: CE
authors.lastname: Hagenaars
authors.forename: E W
authors.initials: EW
authors.lastname: Kawilarang-de Haas
authors.forename: A A
authors.initials: AA
authors.lastname: van der Kraan
authors.forename: E
authors.initials: E
authors.lastname: Spooncer
authors.forename: T M
authors.initials: TM
authors.lastname: Dexter
authors.forename: P J
authors.initials: PJ
authors.lastname: Nijweide
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D007377:Interleukin-3 / Q000494:pharmacology*; D051379:Mice; D008854:Microscopy, Electron; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D013194:Staining and Labeling
chemical_list: D003470:Culture Media; D007377:Interleukin-3; D000135:Acid Phosphatase; D002118:Calcium