Selective expression of Jun proteins following axotomy and axonal transport block in peripheral nerves in the rat: evidence for a role in the regeneration process.
Expression of the protein products of the immediate-early genes (IEGs), members of the fos, jun and krox families (Jun, Fos, and Krox, resp.) was investigated in the spinal cord and sensory ganglia (DRG) of normal rats; and following transection of, block of axonal transport in, or electrical stimulation of their peripheral axons. The nuclei of many moto- and DRG neurons showed a faint basal immunoreactivity (IR) for Jun proteins, but not for Fos or Krox proteins. There was a strong and selective induction of Jun-IR in moto- and DRG neurons after peripheral nerve transection or crush, or colchicine- or vinblastine-induced block of axonal transport. The Jun-IR induced by nerve transection disappeared after nerve regeneration. In contrast, Jun, Fos and Krox proteins were all induced transynaptically in spinal dorsal horn neurons following electrical stimulation of the C-fibers in the afferent nerves. Thus in differentiated neurons in vivo these IEG proteins can be expressed either independently or concomitantly depending on the type of stimulus.
Leah
J D
JD
School of Science, Griffith University, Brisbane, Australia.
Herdegen
T
T
Bravo
R
R
D000818:Animals; D000906:Antibodies; D001370:Axonal Transport; D001369:Axons / Q000502:physiology*; D003078:Colchicine / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis; D005727:Ganglia, Spinal / Q000502:physiology; D016762:Genes, fos; D016758:Genes, jun; D007700:Kinetics; D008297:Male; D009046:Motor Neurons / Q000502:physiology; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D014176:Protein Biosynthesis; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000096:biosynthesis; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000502:physiology*; D013116:Spinal Cord / Q000502:physiology*; D014747:Vinblastine / Q000494:pharmacology
D000906:Antibodies; D004268:DNA-Binding Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D014747:Vinblastine; D003078:Colchicine