[Properties of mitochondrial porin from cattle heart].
Porin, a protein able to form ionic channels in model phospholipid membranes, has been isolated for the first time from bovine heart mitochondria. One-dimensional electrophoresis in the presence of sodium dodecyl sulfate revealed a major band with Mr of 32-34 kDa. On two-dimensional electrophoregrams this protein is represented by four components with pI ranging from 6.5 to 7.1. Porin spots were identified on two-dimensional electrophoregrams in a complete mixture of mitochondrial proteins. The presence of porin in bovine heart submitochondrial particles was demonstrated by two-dimensional electrophoresis.
Konstantinova
S A
SA
Zorov
D B
DB
Kovalev
L I
LI
Shishkin
S S
SS
D000818:Animals; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007473:Ion Channels / Q000378:metabolism; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D018272:Porins; D050994:Voltage-Dependent Anion Channels
D007473:Ion Channels; D008565:Membrane Proteins; D018272:Porins; D050994:Voltage-Dependent Anion Channels
Izuchenie svoĭstv mitokhondrial'nogo porina iz serdtsa byka.