Chimeric cytotoxin IL2-PE40 inhibits relapsing experimental allergic encephalomyelitis.
IL2-PE40 is a chimeric protein composed of human interleukin-2 (IL2) genetically fused to a modified form of Pseudomonas exotoxin lacking the cell recognition domain. IL2-PE40 is cytotoxic for IL2 receptor-bearing lymphocytes in culture and can inhibit activation of T cells in vivo. IL2-PE40 can significantly diminish antigen-stimulated proliferation of lymphocytes sensitized to myelin basic protein. Intraperitoneal administration of IL2-PE40 not only markedly inhibits the clinical manifestations of adoptively transferred relapsing experimental allergic encephalomyelitis but also dramatically reduces both inflammation and demyelination characteristic of the disease.
Rose
J W
JW
Neurovirology Research Laboratory VAMC, Salt Lake City, UT 84148.
Lorberboum-Galski
H
H
Fitzgerald
D
D
McCarron
R
R
Hill
K E
KE
Townsend
J J
JJ
Pastan
I
I
D000818:Animals; D001426:Bacterial Proteins / Q000627:therapeutic use*; D001921:Brain / Q000473:pathology; D003711:Demyelinating Diseases / Q000517:prevention & control; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology / Q000517:prevention & control*; D005098:Exotoxins / Q000627:therapeutic use*; D005260:Female; D006168:Guinea Pigs; D016219:Immunotherapy, Adoptive; D000922:Immunotoxins / Q000627:therapeutic use*; D007376:Interleukin-2 / Q000627:therapeutic use*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D004676:Myelin Basic Protein / Q000276:immunology; D011550:Pseudomonas aeruginosa; D015375:Receptors, Interleukin-2 / Q000032:analysis; D011994:Recombinant Proteins
D001426:Bacterial Proteins; D005098:Exotoxins; C057961:IL-2-PE40 chimeric protein, recombinant; D000922:Immunotoxins; D007376:Interleukin-2; D004676:Myelin Basic Protein; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins