Replacement of donor lymphoid tissue in small-bowel transplants.
The presence of recipient lymphocytes in grafts is thought to equate with rejection. Thus, we wished to follow the fate of lymphocytes after transplant of the small bowel. Three complete small-bowel transplants, two with the liver from the same donor also transplanted, were done successfully. Patients were immunosuppressed with FK 506. 5 to 11% of lymphocytes in the recipients' peripheral blood were of donor origin during the early postoperative period when there were no clinical signs of graft-versus-host disease. However, donor cells were no longer detectable after 12 to 54 days. Serial biopsy specimens of the grafted small bowel showed progressive replacement of lymphocytes in the lamina propria by those of the recipient's HLA phenotype. Lymphoid repopulation was complete after 10 to 12 weeks but the epithelial cells of the intestine remained those of the donor. The patients are on enteral alimentation after 5, 6, and 8 months with histopathologically normal or nearly normal intestines. Re-examination of assumptions about the rejection of intestinal grafts and strategies for its prevention are required following these observations.
Iwaki
Y
Y
Department of Surgery, University of Pittsburgh School of Medicine, Pennsylvania.
Starzl
T E
TE
Yagihashi
A
A
Taniwaki
S
S
Abu-Elmagd
K
K
Tzakis
A
A
Fung
J
J
Todo
S
S
D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D003131:Combined Modality Therapy; D005069:Evaluation Studies as Topic; D005500:Follow-Up Studies; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007421:Intestine, Small / Q000098:blood supply / Q000637:transplantation*; D007958:Leukocyte Count; D008214:Lymphocytes / Q000166:cytology; D008221:Lymphoid Tissue / Q000098:blood supply / Q000637:transplantation*; D012086:Reoperation; D016559:Tacrolimus; D014181:Transplantation Immunology
D000900:Anti-Bacterial Agents; D007166:Immunosuppressive Agents; D016559:Tacrolimus