Naturally occurring aliphatic polyamines-induced histamine release from rat peritoneal mast cells.
Rat peritoneal mast cells were incubated with different concentrations of naturally occurring aliphatic polyamines, spermine and spermidine, at 0.1-10 mM and the amount of histamine release into the supernatant solutions was measured. The addition of each polyamine to the suspensions of the mast cells caused a histamine release in a dose-dependent manner. The effect of 10 mM spermine and spermidine was as much as that of 0.5 microgram/ml compound 48/80. The histamine release from the cells incubated with each polyamine was rapid and the amount of histamine release into the supernatant solutions reached a maximum at 1 min with the incubations. 0.1 mM spermine, which in itself could not cause a significant histamine release, showed a tendency to enhance anti-IgE-induced histamine release from the mast cells.
Kurosawa
M
M
First Department of Internal Medicine, Gunma University School of Medicine, Japan.
Uno
D
D
Kobayashi
S
S
D000818:Animals; D002478:Cells, Cultured; D006636:Histamine Release / Q000187:drug effects*; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D011073:Polyamines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D011073:Polyamines; D013096:Spermine; D013095:Spermidine