title: Location of the quinine-dependent epitope on platelet glycoprotein IIIa.
abstract: Quinine-dependent (Q) IgG antibodies (Q.Ab) in drug-induced immune thrombocytopenia are heterogeneous and bind to different platelet surface glycoproteins (GP), namely GPIb, IX, IIb, IIIa and an unidentified 57-kDa membrane proteins. Although both the Q-dependent epitope on GPIIIa and the P1A1 antigen require intact disulphide bonds for their expression, they are distinct because Q.Ab bind to GPIIIa lacking P1A1. Epitopes for both antigens were examined on Western blots of either intact washed human platelets or purified GPIIIa. When intact platelets were digested with trypsin and washed and solubilised prior to electrophoresis, membrane-associated fragments of GPIIIa of 78 kDa were found to be reactive with both antibodies. In addition, 60- and 68-kDa fragments bound anti-P1A1 but not Q.Ab. Similar digestion with chymotrypsin produced only 60-kDa fragments containing both epitopes. Digestion of purified GPIIIa with chymotrypsin produced 60-kDa peptides reactive with Q.Ab and anti-P1A1 in immunoblotting studies. Similar digestion with elastase produced 58-kDa fragments also containing the epitopes for both antibodies. Longer digestion times or sequential digestion with different enzymes did not reveal extra fragments. However, immunoprecipitation of trypsin-digested 125I-labelled GPIIIa with affinity-purified Q.Ab produced a 17-kDa fragment containing the Q-dependent epitope.
authors.affiliation: Department of Medicine, Monash University Medical School, Alfred Hospital, Prahran, Vic., Australia.
authors.forename: S L
authors.initials: SL
authors.lastname: Pfueller
authors.forename: W F
authors.initials: WF
authors.lastname: Cortizo
authors.forename: J
authors.initials: J
authors.lastname: Gibson
authors.forename: R
authors.initials: R
authors.lastname: Bilston
authors.forename: I G
authors.initials: IG
authors.lastname: Sloan
mesh_terms: D016824:Antigens, Human Platelet / Q000276:immunology* / Q000302:isolation & purification; D001792:Blood Platelets / Q000737:chemistry* / Q000276:immunology; D010450:Endopeptidases; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D010446:Peptide Fragments / Q000276:immunology / Q000302:isolation & purification; D010980:Platelet Membrane Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D011803:Quinine / Q000009:adverse effects; D013921:Thrombocytopenia / Q000139:chemically induced / Q000276:immunology
chemical_list: D016824:Antigens, Human Platelet; D000939:Epitopes; D010446:Peptide Fragments; D010980:Platelet Membrane Glycoproteins; D011803:Quinine; D010450:Endopeptidases