title: Responses of T cells from sensitized donors to recombinant and synthetic peptides corresponding to sequences of the Plasmodium falciparum SERP antigen.
abstract: In the present work, we intend to determine the capacity of human lymphocytes to recognize subfragments of the serine-stretch protein SERP, a blood-stage antigen from Plasmodium falciparum. Individuals sensitized by a previous P. falciparum infection were studied. Some recombinant proteins (RP) including RP7 and RP10 (amino acids 631-684 and 631-892 of SERP, respectively), were recognized in proliferation assays by lymphocytes from 28 sensitized individuals and not by lymphocytes from control, non-sensitized, donors. Synthetic peptides covering predefined zones of particular interest were tested and appeared to induce proliferative responses of lymphocytes from sensitized donors, allowing identification of putative T cell epitopes.
authors.affiliation: Unité INSERM U 108, Hôpital Saint Louis, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Roussilhon
authors.forename: E
authors.initials: E
authors.lastname: Hundt
authors.forename: M
authors.initials: M
authors.lastname: Agrapart
authors.forename: W
authors.initials: W
authors.lastname: Stüber
authors.forename: B
authors.initials: B
authors.lastname: Knapp
authors.forename: P
authors.initials: P
authors.lastname: Dubois
authors.forename: J J
authors.initials: JJ
authors.lastname: Ballet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D000939:Epitopes; D010446:Peptide Fragments; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C057495:serine repeat antigen, Plasmodium