Decarboxylation of exogenous L-3,4-dihydroxyphenylalanine in rat striatum as studied by in vivo voltammetry.
An in vivo voltammetric technique was used to determine whether striatal nondopaminergic neurons take up and decarboxylate exogenous L-3,4-dihydroxyphenylalanine (L-DOPA) and release it as dopamine. After the striatal serotonergic neurons of the rat had been destroyed by intraventricular injection of 5,7-dihydroxytryptamine, L-DOPA was administered intraperitoneally. It was found that changes in the dopamine concentration in the striatal extracellular fluid of the rat were the same as those in the nonlesioned rat. L-DOPA was also administered to the rat after the striatal perikarya had been destroyed by the intrastriatal injection of kainate. The striatal dopamine concentrations of the lesioned rat changed in parallel with 5,7-dihydroxytryptamine-lesioned rats, as well as the nonlesioned rats. Moreover, when normal rats were administered L-DOPA, the dopamine concentration was not increased in the cerebellum, where dopamine neurons do not exist. From these observations, it is concluded that exogenous L-DOPA is taken up, decarboxylated to dopamine, and released only in the striatal dopamine neurons.
Nakazato
T
T
Department of Physiology, Juntendo University School of Medicine, Tokyo, Japan.
Akiyama
A
A
D015116:5,7-Dihydroxytryptamine / Q000494:pharmacology; D000818:Animals; D002531:Cerebellum / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D003653:Decarboxylation; D004563:Electrochemistry / Q000379:methods; D004567:Electrodes, Implanted; D007980:Levodopa / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D015116:5,7-Dihydroxytryptamine; D007980:Levodopa