Mouse LFA-3 studied with chimeric soluble CD2 shows preferential expression on lymphoid cells.
Here we have used a soluble, polyvalent form of mouse CD2, i.e. CD2-hC mu, to detect its ligand LFA-3. Mouse LFA-3 is preferentially expressed on lymphoid cells unlike human LFA-3 which shows a wide tissue distribution. Mouse LFA-3 is one of the early surface molecules expressed on developing thymocytes appearing on the surface of fetal thymocytes on day 13 of gestation. Like human LFA-3, the mouse homolog could be shown to be a phosphatidyl inositol-linked membrane protein. Since mouse LFA-3 is preferentially expressed on lymphoid cells and since CD2 is expressed by both T and B lymphocytes, we would favor the view that the CD2/LFA-3 adhesion system in the mouse may play a role in interactions between lymphocytes (T-T and/or T-B) rather than in cell interactions between lymphocytes and non-lymphoid cells in the thymic, bone marrow or spleen microenvironments.
Rutschmann
R
R
Basel Institute for Immunology, Switzerland.
Karjalainen
K
K
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000378:metabolism*; D000954:Antigens, Surface / Q000032:analysis*; D018801:CD2 Antigens; D018968:CD58 Antigens; D002450:Cell Communication; D005260:Female; D005333:Fetus / Q000276:immunology; D005434:Flow Cytometry; D008562:Membrane Glycoproteins / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D010716:Phosphatidylinositols / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000276:immunology*; D010738:Type C Phospholipases / Q000494:pharmacology
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D018801:CD2 Antigens; D018968:CD58 Antigens; D008562:Membrane Glycoproteins; D010716:Phosphatidylinositols; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; D010738:Type C Phospholipases