title: The crucial role of cyclic GMP in the eclosion hormone mediated signal transduction in the silkworm metamorphoses.
abstract: The signal transduction of the peptide, eclosion hormone, in the silkworm Bombyx mori appears to be mediated via the second messenger cyclic GMP throughout their life cycle. Injection of 8-bromo-cGMP induced the ecdysis behavior in pharate adults with similar latency to eclosion hormone-induced ecdysis; the moulting occurred 50-70 min after the injection. The potency of 8Br-cGMP was 10(2) fold higher than that of cGMP and the efficacy was increased by the co-injection of the phosphodiesterase inhibitor IBMX. On the other hand, in the silkworm pupal ecdysis the eclosion hormone and also 8Br-cGMP induced the moulting behavior in a dose-dependent manner. The adult development of the ability to respond to 8Br-cGMP took place concomitantly with the response to the eclosion hormone. Both the developmental time courses were shifted by a shift of light and dark cycles. Accordingly, the sensitivities to the peptide and cyclic nucleotide developed correspondently under the light and dark circadian rhythm. Thus throughout the silkworm life cycle, eclosion hormone is effective to trigger the ecdysis behavior and cGMP plays a crucial role as the second messenger in the eclosion hormone-mediated signal transduction.
authors.affiliation: Bio-organics Research Department, International Research Laboratories, CIBA-GEIGY Ltd., Takarazuka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shibanaka
authors.forename: H
authors.initials: H
authors.lastname: Hayashi
authors.forename: N
authors.initials: N
authors.lastname: Okada
authors.forename: N
authors.initials: N
authors.lastname: Fujita
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology*; D000818:Animals; D012831:Bombyx / Q000187:drug effects / Q000502:physiology*; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D007301:Insect Hormones / Q000494:pharmacology* / Q000502:physiology; D008675:Metamorphosis, Biological / Q000187:drug effects; D009036:Moths; D011994:Recombinant Proteins / Q000494:pharmacology; D015290:Second Messenger Systems / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D007301:Insect Hormones; D011994:Recombinant Proteins; C016276:8-bromocyclic GMP; C042645:eclosion hormone; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine