title: Epitope mapping of HBsAg using a panel of human anti-HBs antibodies.
abstract: Mapping of B cell epitopes on HBsAg was performed using a panel of human anti-HBs antibodies. Synthetic peptides representing different regions of HBsAg failed to inhibit the binding of two antibodies which recognized non-conformational HBsAg determinants in dot-blot ELISA and HBsAg polypeptide bands in immunoblot analysis. Cross-inhibition studies using five of the antibodies conjugated to horseradish peroxidase suggested that at least three different epitopes are recognised by the panel of antibodies, two of which are within the 'a' group determinant.
authors.affiliation: Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, U.K.
authors.forename: A
authors.initials: A
authors.lastname: Sa'adu
authors.forename: D
authors.initials: D
authors.lastname: Bidwell
authors.forename: M
authors.initials: M
authors.lastname: Locniskar
authors.forename: C
authors.initials: C
authors.lastname: Howard
authors.forename: K P
authors.initials: KP
authors.lastname: McAdam
authors.forename: A
authors.initials: A
authors.lastname: Voller
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D006510:Hepatitis B Antibodies / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology; D008969:Molecular Sequence Data; D009842:Oligopeptides
chemical_list: D000939:Epitopes; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007074:Immunoglobulin G; D009842:Oligopeptides