Immunohistochemical and immunochemical detection of a similar epitope in enamel proteins and monocyte-macrophage protein recognized by mouse monoclonal antibody MOMA-2.
These studies were made as a first step in elucidating unknown functions of enamel proteins in odontogenesis. The cytoplasm of ameloblasts and the proteins in dentine matrix before mineralization were stained with this monoclonal antibody. SDS-PAGE and Coomassie blue staining of proteins extracted from enamel showed several protein bands. Immunoblotting revealed that proteins recognized by this antibody were situated between 20-30 kDa. These results indicate that enamel proteins, presumably amelogenins, have an epitope resembling monocyte-macrophage protein. The findings suggest that epithelial-mesenchymal interaction in odontogenesis and preosteoblast-preosteoclast interaction in osteogenesis may be similar.
Nakamura
M
M
Second Department of Oral Anatomy, School of Dentistry, Tohoku University, Sendai, Japan.
Kindaichi
K
K
Kagayama
M
M
D000565:Ameloblasts / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D003746:Dental Enamel Proteins / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004658:Enamel Organ / Q000737:chemistry; D000939:Epitopes / Q000032:analysis*; D007120:Immunochemistry; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000378:metabolism*; D009804:Odontoblasts / Q000378:metabolism; D009805:Odontogenesis; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D012967:Sodium Dodecyl Sulfate
D000911:Antibodies, Monoclonal; D003746:Dental Enamel Proteins; D000939:Epitopes; D011506:Proteins; D012967:Sodium Dodecyl Sulfate