Washless double staining of a nuclear antigen (Ki-67 or bromodeoxyuridine) and DNA in unfixed nuclei.
Larsen
J K
JK
Finsen Laboratory, Righospitalet, University Hospital, Copenhagen, Denmark.
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine; D002467:Cell Nucleus / Q000276:immunology*; D004247:DNA / Q000032:analysis*; D003902:Detergents; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D019394:Ki-67 Antigen; D009687:Nuclear Proteins / Q000032:analysis*; D011419:Propidium; D013194:Staining and Labeling / Q000379:methods*; D013861:Thiocyanates
D000911:Antibodies, Monoclonal; D003902:Detergents; D005452:Fluoresceins; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D013861:Thiocyanates; D011419:Propidium; D004247:DNA; D001973:Bromodeoxyuridine; D016650:Fluorescein-5-isothiocyanate