[Virus inactivation of plasma].
A procedure for the virus inactivation of single donor plasma is described. Virus inactivation is achieved by the combination of 1 mumol of phenothiazin dye, e.g. methylene blue and toluidine blue, and visible light. Using this method VSV, HSV and HIV, as well as other viruses, were inactivated. It could be shown that virus inactivation caused only a minimal reduction in the biological activities of coagulation factors and inhibitors. Furthermore, there was no indication for either neo-antigens or IgE-antibodies. In tolerance studies on dogs it was demonstrated that virus-inactivated autologous plasma caused no toxic effects.
Schmitt
H
H
Zentralinstitut Springe, DRK-Blutspendedienst Niedersachsen, BRD.
Mohr
H
H
Lambrecht
B
B
Knüver-Hopf
J
J
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001779:Blood Coagulation Factors / Q000032:analysis; D004285:Dogs; D006801:Humans; D008751:Methylene Blue / Q000627:therapeutic use; D010789:Phototherapy; D010949:Plasma / Q000382:microbiology*; D011817:Rabbits; D014775:Virus Activation / Q000187:drug effects*
D000998:Antiviral Agents; D001779:Blood Coagulation Factors; D008751:Methylene Blue
Virusinaktivierung von Plasma.