[The heterogeneity of an influenza virus A population due to differences at individual hemagglutinin H3 sites].
Using monoclonal antibodies to hemagglutinin and nucleoprotein of various influenza virus strains, the populations of long-passaged strain A/Hong Kong/1/68 and of recently isolated strain A/sparrow/Ukraine/83 belonging to the H3N2 serovariant were shown to have subpopulations of virions differing in the structure of antigenic sites of hemagglutinin and in nucleoprotein domain which correlated with the degree of electrostatic interaction of virions with an ion-exchanger. The results of the study indicate a possibility of separation in the course of stepwise ion-exchange chromatography on DEAE-Sephadex A-50 of antigenic variants of influenza virus strains which is very important for the understanding of the mechanisms of population variability as well as for investigation of individual epitopes of hemagglutinin and nucleoprotein domains of influenza virus in evaluation of antigenic relationships of virions belonging to the same strain of a certain serotype.
Sidorenko
E V
EV
Iamnikova
S S
SS
Ignatenko
T A
TA
Smogorzhevskiĭ
L A
LA
L'vov
D K
DK
D000818:Animals; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000032:analysis / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods; D012692:Serial Passage
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D006389:Hemagglutinins, Viral
Geterogennost' populiatsii virusa grippa A, obuslovlennaia otlichiiami v otdel'nykh saĭtakh gemaggliutinina H3.