title: Virus, bacteria and lipopolysaccharide increase basophil cell response to histamine releasing stimulators and calcium. Examination of allergic and normal individuals.
abstract: Histamine release from human basophil leukocytes from allergic patients or controls was induced by specific antigens, anti-IgE or calcium ionophore A23187. Influenza A virus, S. aureus and lipopolysaccharide from S. typhimurium increased the maximum release of histamine and caused a shift to the left of the dose-response curves showing increased cell sensitivity and lowering of the threshold to these stimuli. The mechanism of action was elucidated by examining the mediator release as a function of increasing extracellular concentration of calcium. In these experiments the dose-response curves were changed by the microorganisms and lipopolysaccharide as before. This indicates that the microorganisms and lipopolysaccharide change the basophil cell response to IgE-dependent and non-immunological stimuli by causing a change in the subcellular handling of calcium.
authors.affiliation: Department of Pharmacology, University of Copenhagen, Denmark.
authors.forename: P
authors.initials: P
authors.lastname: Clementsen
authors.forename: K S
authors.initials: KS
authors.lastname: Kristensen
authors.forename: S
authors.initials: S
authors.lastname: Norn
mesh_terms: D000328:Adult; D001491:Basophils / Q000276:immunology* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D004306:Dose-Response Relationship, Immunologic; D006636:Histamine Release; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008875:Middle Aged; D012486:Salmonella typhimurium / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D008070:Lipopolysaccharides; D007073:Immunoglobulin E; D002118:Calcium