Fatty acid profile and acid phosphatase activity of fresh isolates of Pseudomonas pseudomallei.
Eighty-one fresh isolates of Pseudomonas pseudomallei from melioidosis patients were subjected to the analysis for the fatty acid composition by gas-liquid chromatography (GLC) and pH-dependent pattern of nonspecific phosphatase activity. All the test strains were identical in the GLC profile showing the three peaks of characteristic hydroxy acids (3-OH 14:0, 2-OH 16:0, 3-OH 16:0) and the two prominent peaks of cyclopropane acids (17:0 delta, 19:0 delta). They had also basically the same pH-dependent curves of the enzymatic activity with paranitrophenyl phosphate as substrate, showing two to three peaks or shoulders only in the acidic side of the curve. These two biochemical characteristics could differentiate P. pseudomallei distinctly from P. aeruginosa, but not from P. cepacia.
Kondo
E
E
National Institute of Health, Department of Medical Sciences, 88/7 Soi Bamrasnaradura Hospital, Nonthaburi, Thailand.
Naigowit
P
P
Phanichruttiwong
P
P
Petkanchanapong
W
W
Chetanachan
P
P
Thirawattanasuk
N
N
Kanai
K
K
D000135:Acid Phosphatase / Q000032:analysis*; D016956:Burkholderia cepacia / Q000737:chemistry* / Q000201:enzymology / Q000302:isolation & purification; D016957:Burkholderia pseudomallei / Q000737:chemistry* / Q000201:enzymology / Q000302:isolation & purification; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D006801:Humans; D008554:Melioidosis / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000201:enzymology / Q000302:isolation & purification
D005227:Fatty Acids; D000135:Acid Phosphatase