Absence of phosphatidylinositol (PI)-linked proteins in a very early human multipotential haematopoietic marrow cell.
A very early human haematopoietic progenitor cell population which was negative for the major histocompatibility class II antigen (HLA-DR) and positive for the CD34 (MY10) antigen was separated into two subsets according to the expression of decay-accelerating factor (DAF) on the cell surface. Using immunoadherence, cell cycle analysis, and cell culture, we determined that there is a DAF- multipotential cell and a more differentiated DAF+ lineage specific progenitor cell existing in human bone marrow. The DAF- subset was highly enriched for CFU-GEMM, while the DAF+ subset contained only BFU-E and CFU-GM. The DAF- subset was approximately 0.03% and the DAF+ subset approximately 0.008% of the original bone marrow population. MIRL (membrane-inhibitory of reactive lysis), another PI-linked protein, was not expressed on the DAF- population but was expressed on the DAF+ cells. These observations indicate that PI-linked proteins are absent from the multipotential stem cell but are present on an early lineage specific cell. The absence of expression of PI-linked proteins can be used to further isolate and characterize a very early multipotential haematopoietic progenitor cell population.
Huang
A T
AT
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
Panella
T J
TJ
Mold
N G
NG
Rosse
W F
WF
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D000943:Antigens, Differentiation / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D018958:CD55 Antigens; D002453:Cell Cycle; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003169:Complement Inactivator Proteins / Q000032:analysis*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*
D015703:Antigens, CD; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D001798:Blood Proteins; D018958:CD55 Antigens; D003169:Complement Inactivator Proteins; D008565:Membrane Proteins