Lung to blood passage of different-sized molecules during lung inflammation in the rat.
The passage of different-sized marker molecules over the lower respiratory tract into the blood circulation during pulmonary inflammation induced by dextran, endotoxin [i.e., lipopolysaccharide from Escherichia coli (LPS)], or ferritin was assessed in the rat. Bovine immunoglobulin G (BIgG, mol wt = 150,000 Da), bovine serum albumin (BSA, mol wt = 67,000 Da), and the nonapeptide 1-deaminocysteine-8-D-arginine vasopressin (dDAVP, mol wt = 1,067 Da) were used as permeability markers after intratracheal instillation. The pathophysiological indexes of a proceeding lung inflammation were increased total cell number, changed leukocyte proportions and increased total protein content obtained in bronchoalveolar lavage, and lung edema formation shown as an increased lung wet-dry weight difference. Intratracheal instillation of dextran induced a moderate neutrophil invasion into the lungs but had no effect on the passage of the different markers over the lungs (BIgG 1.8 +/- 0.6%, BSA 3.5 +/- 1.2%, dDAVP 26.1 +/- 20.7%) compared with control rats instilled with the markers alone (1.8 +/- 0.4%, 4.1 +/- 1.3%, 20.0 +/- 3.8%, respectively). Endotoxin administration resulted in markedly higher lavage cell counts and lung edema concomitantly with an increased lung passage of the markers (3.2 +/- 0.9%, 22.0 +/- 6.1%, 33.3 +/- 12.0%, respectively; P less than 0.01-P less than 0.001). The highest marker passage was obtained when the inflammation was most severe, i.e., after ferritin administration (17.6 +/- 2.3%, 60.0 +/- 6.7%, 41.6 +/- 6.9%, respectively; P less than 0.001), which resulted in markedly elevated lavage cell numbers and protein content as well as edema formation.(ABSTRACT TRUNCATED AT 250 WORDS)
Folkesson
H G
HG
Department of Animal Physiology, University of Lund, Sweden.
Weström
B R
BR
Pierzynowski
S G
SG
Karlsson
B W
BW
D000818:Animals; D015415:Biomarkers; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D003894:Deamino Arginine Vasopressin; D003676:Deferoxamine / Q000494:pharmacology; D003911:Dextrans / Q000633:toxicity; D005293:Ferritins / Q000633:toxicity; D007074:Immunoglobulin G; D007442:Intubation, Intratracheal; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000378:metabolism*; D011014:Pneumonia / Q000097:blood / Q000139:chemically induced / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine
D015415:Biomarkers; D003911:Dextrans; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D011506:Proteins; D012710:Serum Albumin, Bovine; D005293:Ferritins; D003894:Deamino Arginine Vasopressin; D003676:Deferoxamine