title: [Synthesis of RNA using T7 RNA polymerase and immobilized DNA in a stream type reactor].
abstract: The DNA ligase-induced assembly of synthetic oligodeoxyribonucleotides on polymer supports was used to obtain immobilized DNA, containing the T7 RNA polymerase promoter and coding for a 14-membered oligoribonucleotide. The obtained template can carry out RNA synthesis in a flowing-type reactor. Sepharose 4B and Toyopearl HW-55 were used as supports.
authors.forename: A A
authors.initials: AA
authors.lastname: Elov
authors.forename: E M
authors.initials: EM
authors.lastname: Volkov
authors.forename: Z A
authors.initials: ZA
authors.lastname: Shabarova
mesh_terms: D001483:Base Sequence; D002853:Chromatography, Liquid; D004247:DNA / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012313:RNA / Q000138:chemical synthesis*; D013604:T-Phages / Q000201:enzymology*; D014158:Transcription, Genetic
chemical_list: D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases
vernacular_title: Sintez RNK s pomoshch'iu RNK-polimerazy T7 i immobilizovannoĭ DNK v reaktore protochnogo tipa.