Conformational difference of T cell antigen receptors revealed by monoclonal antibodies to mouse V beta 5 T cell receptor for antigen determinants.
The mAb MR9-4 and MR9-8 react with T cells expressing the V beta 5.1 and -5.2 chains of the TCR. T cells expressing V beta 5.1 TCR were stained by both antibodies with similar surface fluorescence intensity. For the T cell clones and hybridomas expressing V beta 5.2 TCR, staining intensity with MR9-8 varied from negative to comparable to that stained with the anti-pan V beta 5 mAb MR9-4, whereas every V beta 5-positive T cell can be activated with either MR9-4 or -9-8 mAb, suggesting a differential binding affinity of MR9-8 mAb to V beta 5 TCR molecules. Analysis of J beta segment and V alpha chain usage in the V beta 5-positive T cell hybridomas revealed that a differential binding of MR9-8 mAb to the V beta 5.2 chain is not dependent on either the J beta segment usage or the associating V alpha chain alone. These results suggest that the differential binding of MR9-8 mAb to V beta 5.2 TCR is due to the conformational change of the V beta chain created by a combination of the V alpha (possibly J alpha) and D beta-J beta segment associating with the V beta 5.2 chain.
Kanagawa
O
O
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
Utsunomiya
Y
Y
Bill
J
J
Palmer
E
E
Moore
M W
MW
Carbone
F R
FR
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D011487:Protein Conformation; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry* / Q000276:immunology / Q000502:physiology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011948:Receptors, Antigen, T-Cell