[Role of leukocytes and amebic proteases in experimental testicular necrosis produced in the rat by Entamoeba histolytica].
We examined the participation of polymorphonuclear leucocytes and amebic proteinases upon tissue damage by means of an experimental model of acute amebic lesions developed in rat's testicle. In leukopenic rats (less than 1,000 leucocytes/ml) intratesticular injection of axenic E. histolytica's trophozoites (HM-1) produced lesions undistinguishable from the normal controls. On the other hand, inhibition of 80% (average) of the proteinase activity by means of previous incubation of the trophozoites with human a2M gave way to minimal inflammatory lesions almost undistinguishable from the controls which were injected with PBS-A. Our data suggest that in this experimental model of acute amebiasis polymorphonuclear leukocytes do not participate in the tissue damage and that amebic proteinases are responsible for Entamoeba histolytica's virulence.
Pérez-Tamayo
R
R
Subdivisión de Medicina Experimental, Facultad de Medicina, U.N.A.M., D.F.
Becker
I
I
Montfort
I
I
Pérez-Montfort
R
R
D000818:Animals; D010450:Endopeptidases / Q000502:physiology*; D004748:Entamoeba histolytica / Q000201:enzymology / Q000472:pathogenicity*; D004749:Entamoebiasis / Q000150:complications / Q000201:enzymology / Q000473:pathology*; D007970:Leukopenia / Q000139:chemically induced / Q000150:complications; D008297:Male; D008466:Mechlorethamine / Q000633:toxicity; D009336:Necrosis; D009504:Neutrophils / Q000502:physiology*; D009920:Orchitis / Q000150:complications / Q000469:parasitology* / Q000473:pathology; D011480:Protease Inhibitors / Q000494:pharmacology; D015800:Protozoan Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014774:Virulence; D000511:alpha-Macroglobulins / Q000494:pharmacology
D011480:Protease Inhibitors; D015800:Protozoan Proteins; D000511:alpha-Macroglobulins; D008466:Mechlorethamine; D010450:Endopeptidases
Papel de los leucocitos y de las proteinasas amibianas en la necrosis testicular experimental producida en la rata por Entamoeba histolytica.