Stimulation of frog ciliated cells in culture by acetylcholine and substance P.
1. Ciliary beat frequency in epithelial outgrowths from cultured explants of Rana pipiens palate changed markedly from second to second. 2. Acetylcholine (10(-8) to 10(-3) M) and substance P (1.35 x 10(-7) to 1.35 x 10(-5) M) increased and stabilized ciliary beat frequency. The effect of acetylcholine and part of the effect of substance P were blocked by atropine (10(-4) M). 3. Acetylcholine appears to act directly and substance P both directly and indirectly through the release of acetylcholine.
Aiello
E
E
Department of Biological Sciences, Fordham University, Bronx, NY 10458.
Kennedy
J
J
Hernandez
C
C
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D002478:Cells, Cultured; D002923:Cilia / Q000187:drug effects* / Q000502:physiology; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D008853:Microscopy / Q000379:methods; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D009079:Mucociliary Clearance / Q000187:drug effects; D009093:Mucus / Q000378:metabolism; D010159:Palate / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D011894:Rana pipiens; D013268:Stimulation, Chemical; D013373:Substance P / Q000494:pharmacology*; D014741:Video Recording
D013373:Substance P; D000109:Acetylcholine; D002211:Capsaicin