title: Occupational allergy to pancreatic powder: characterization of IgE-binding antigens in pancreatic extract by immunoblotting.
abstract: The IgE response to inhaled dust of pancreatic powder was studied with sera from two employees of a pharmacy. IgE from both sera was directed against both porcine and bovine trypsin preparations. The major IgE-binding structures had an isoelectric point in the pH 8 to 9 range. The molecular weight of the IgE-binding structures was different for both patients. In one patient, most IgE was directed against a 28 to 30 kd structure, whereas the second patient had IgE directed against components with a molecular weight of 35 and 45 kd, respectively.
authors.affiliation: Department of Clinical Chemistry (Laboratory of Allergology), University Hospital-Dijkzigt, Rotterdam, The Netherlands.
authors.forename: A W
authors.initials: AW
authors.lastname: van Toorenenbergen
authors.forename: M I
authors.initials: MI
authors.lastname: Huijskes-Heins
authors.forename: P H
authors.initials: PH
authors.lastname: Dieges
authors.forename: B
authors.initials: B
authors.lastname: Leijnse
mesh_terms: D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006636:Histamine Release; D006801:Humans; D015151:Immunoblotting; D007122:Immunoelectrophoresis; D007073:Immunoglobulin E / Q000276:immunology*; D016273:Occupational Exposure; D010184:Pancreatic Extracts / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000139:chemically induced / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D010184:Pancreatic Extracts; D007073:Immunoglobulin E