Human elongation factor 1 beta: cDNA and derived amino acid sequence.
From a cDNA library in lambda gt11 derived from poly (A+)RNA of human ovarian granulosa cells a cDNA clone lambda HGP34, containing an EcoRI insert of 829 bp, was identified. After subcloning of the insert into pUC18, the clone pHGP34 was obtained and sequenced. The derived amino acid sequence, corresponding to a protein of 225 amino acids, shows a high degree of homology to elongation factor 1 beta (EF-1 beta) of Artemia salina (57%) and known peptide sequences of Xenopus laevis EF-1 beta (86%). We therefore assume that the protein coded for by pHGP34 represents human EF-1 beta. Northern analysis reveals an EF-1 beta specific mRNA of 900 bp. Southern analysis indicates that EF-1 beta in the human genome, like EF-1 alpha, appears to be specified by more than one gene. A high degree of sequence homology for EF-1 beta specific sequences is observed for bovine, rat and mouse species.
von der Kammer
H
H
Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Biologie, Göttingen, Germany.
Klaudiny
J
J
Zimmer
M
M
Scheit
K H
KH
D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D015723:Gene Library; D006107:Granulosa Cells / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012261:Ribonucleoproteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D012261:Ribonucleoproteins; D012313:RNA; D004247:DNA