A histochemical method for the demonstration of unsaturated lipids.
A method has been developed for the histochemical demonstration of unsaturated lipids in light microscopy. It is a peracetic acid-thiocarbohydrazide-silver protein sequence followed by a physical development procedure. In the present study on paraffin and cryostat sections of liver, brain and ovary, unsaturated lipids were visualized as distinct reaction products coloured various shades of brown and black. The reaction products are easier to see and the method is more efficient than the peracetic acid-Schiff method.
Fujimori
O
O
Department of Anatomy, Nagoya City University Medical School, Japan.
Ueda
H
H
Yamada
K
K
D000818:Animals; D001923:Brain Chemistry; D005224:Fats, Unsaturated / Q000032:analysis*; D005260:Female; D006651:Histocytochemistry / Q000379:methods*; D006834:Hydrazines; D008099:Liver / Q000737:chemistry; D008853:Microscopy; D008867:Microtomy; D010053:Ovary / Q000737:chemistry; D010084:Oxidation-Reduction; D010463:Peracetic Acid / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D012836:Silver Proteins / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods
D005224:Fats, Unsaturated; D006834:Hydrazines; D012836:Silver Proteins; C011368:thiocarbohydrazide; D010463:Peracetic Acid