Presence of four tachykinins in an acid extract of the carp intestinal bulb (Cyprinus carpio).
1. The pharmacological and chemical properties of substance P-like peptides isolated from an acid extract of the carp intestinal bulb were examined using guinea-pig ileum longitudinal smooth muscle. 2. On a Sephadex G25 column (3 x 96 cm), smooth muscle contracting material was eluted as two peaks (fraction-1 and fraction-2). The molecular weight of the fraction-1 was estimated to be 2300 and that of the fraction-2 to be 1530. 3. The pharmacological properties of the contracting materials in fraction-1 and fraction-2 resembled those of substance P and neurokinin A. 4. The susceptibility of the contracting activity of fraction-1 to proteolytic enzymes resembled that of physalaemin but, on the other hand, the susceptibility of that of fraction-2 resembled those of eledoisin and neurokinin A. 5. Ion-exchange chromatography on sulfopropyl-Sephadex C25 indicated the presence of one contracting material in fraction-1 and three contracting materials in fraction-2. The elution positions of four materials were different from that of substance P. 6. These results indicate that four tachykinins different from substance P are present in an acid extract of the carp intestinal bulb.
Kitazawa
T
T
Department of Veterinary Pharmacology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Kudo
K
K
Ishigami
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps; D002850:Chromatography, Gel; D010450:Endopeptidases / Q000494:pharmacology; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D007082:Ileum / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D007422:Intestines / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology; D013045:Species Specificity; D013373:Substance P / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology; D015320:Tachykinins / Q000302:isolation & purification* / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology
D015320:Tachykinins; D013373:Substance P; D013779:Tetrodotoxin; C042867:spantide; D010450:Endopeptidases