The analysis of underivatized oligosaccharides by matrix-assisted laser desorption mass spectrometry.
Matrix Assisted Laser Desorption Mass Spectrometry is shown to provide a rapid and sensitive technique for the analysis of underivatized oligosaccharides. Typical sample loading is 1 pmol and analysis time is around 5 minutes. Through the use of an internal standard, mass measurements are generally accurate to within 0.5 Da. The technique is particularly useful for the analysis of oligosaccharide mixtures released from glycoproteins.
Mock
K K
KK
Finnigan MAT Ltd., Paradise, Hemel Hempstead, Herts, UK.
Davey
M
M
Cottrell
J S
JS
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D003911:Dextrans / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D007834:Lasers; D008358:Mannose / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*
D003911:Dextrans; D006023:Glycoproteins; D009844:Oligosaccharides; D008358:Mannose