title: Cellular localization of PNA binding in colorectal adenomas: comparison with differentiation, nuclear:cell height ratio and effect of desialylation.
abstract: The lectin Arachis Hypogaea (Peanut Agglutinin, PNA) was used to study the cellular localization of the Thomsen-Friedenreich (T) disaccharide Gal-beta (1-3)-GalNAc alpha 1-R in 22 formalin-fixed paraplast-embedded colorectal adenomas of varying cellular dysplasia. An indirect immunoperoxidase method was used prior to and after neuraminidase treatment. Detailed information on the cellular localization of PNA binding was obtained. In addition, morphometric measurements of the nuclear: cell height ratios were performed on staining-filtered micrographs of crypts from all adenomas. We found 1) a statistically significant increase in the nuclear:cell height ratio with increasing grade of dedifferentiation (p less than 0.003), 2) a statistically significant smaller nuclear:cell height ratio in crypts that were PNA-positive in the Golgi region when these were compared to crypts that were PNA-positive on luminal cell membranes, 3) a decreasing number of crypts expressing PNA binding sites in the Golgi region with increasing dedifferentiation, leading to complete absence of PNA binding sites in Grade IV adenomas, 4) neuraminidase pretreatment increased the number of crypts expressing PNA binding sites in cytoplasm and on luminal membranes, whereas no changes were detected in crypts expressing PNA binding sites in the Golgi region. Our results confirm the general concept of accumulation of precursors of carbohydrate antigens in dedifferentiated cells. On the basis of the results presented, we conclude that the nuclear:cell height ratio shows a good correlation with the cellular localization of PNA binding, cellular differentiation and classic pathologic grading.
authors.affiliation: Danish Cancer Society, Department of Experimental Clinical Oncology, Aarhus, Denmark.
authors.forename: T F
authors.initials: TF
authors.lastname: Orntoft
authors.forename: N C
authors.initials: NC
authors.lastname: Langkilde
authors.forename: H
authors.initials: H
authors.lastname: Wiener
authors.forename: P D
authors.initials: PD
authors.lastname: Ottosen
mesh_terms: D000236:Adenoma / Q000473:pathology*; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate; D010367:Arachis; D002454:Cell Differentiation; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure*; D003110:Colonic Neoplasms / Q000473:pathology*; D004187:Disaccharides / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins; D008297:Male; D009439:Neuraminidase; D019887:Peanut Agglutinin; D037121:Plant Lectins; D012004:Rectal Neoplasms / Q000473:pathology*; D012794:Sialic Acids / Q000032:analysis; D013194:Staining and Labeling
chemical_list: D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D004187:Disaccharides; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D012794:Sialic Acids; C016467:Thomsen-Friedenreich antigen; D009439:Neuraminidase