Localization of actin and characterization of its isoforms in the hyphae of Neurospora crassa.
The actin of Neurospora crassa wild type Strain St. Lawrence has been purified, characterized and localized. A fungal 43 kDa protein was isolated by affinity chromatography on DNase I-Sepharose. This protein was identified as actin on immunoblots when an anti-actin monoclonal antibody raised against chicken gizzards was used as a probe. After two-dimensional gel electrophoresis three actin isoforms were detected. The distribution of actin in hyphae was examined by FITC-phalloidin staining of formaldehyde fixed hyphae. F-actin was found to be mainly concentrated in the hyphal tips in which it formed a uniform cap. Apical actin could be involved in hyphal morphogenesis, organelle motility and maintenance of polarity.
Barja
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F
Laboratory of General Microbiology, University of Geneva, Switzerland.
Thi
B N
BN
Turian
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G
D000199:Actins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis*; D007536:Isomerism; D009492:Neurospora crassa / Q000648:ultrastructure*; D013194:Staining and Labeling
D000199:Actins; D005656:Fungal Proteins