Decrease in cytokine production by HIV-infected macrophages in response to LPS-mediated activation.
The capacity of human monocytes/macrophages (M/M) infected with a human immunodeficiency virus-1 (HIV-1) isolate to produce several immunomodulating cytokines including interleukin-1 alpha (IL-1 alpha), IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), IL-6, IL-8, and macrophage chemoattractant and activating factor (MCAF) was examined. Although HIV infection itself induced significant increases in the level of mRNAs for IL-1 beta, TNF-alpha, IL-6, and IL-8, the levels of lipopolysaccharide (LPS)-induced mRNAs for IL-1 alpha, IL-1 beta, TNF-alpha, IL-6, IL-8, and MCAF were decreased over those of uninfected LPS-stimulated cells. In addition, HIV-infected M/M produced lower amounts of IL-8 protein, as measured by radioimmunoassay over an 18-day culture period. These results suggest that HIV infection generally suppresses the LPS-inducible cytokine production in human M/M. The impact of the role of these cytokines in the immunity and pathogenesis of HIV-1 infection is discussed.
Tsai
W P
WP
Laboratory of Biochemical Physiology, PRI/DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702-1201.
Hirose
K
K
Nara
P L
PL
Kuang
Y D
YD
Conley
S
S
Li
B Q
BQ
Kung
H F
HF
Matsushima
K
K
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood*; D015152:Blotting, Northern; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008262:Macrophage Activation / Q000187:drug effects*; D009000:Monocytes / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000235:genetics
D016207:Cytokines; D012313:RNA