CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x.
The leukocyte receptor CD62, which is expressed on activated platelets and endothelial cells, is shown to mediate cell adhesion by binding a sialylated carbohydrate structure, sialyl-Lewis x, found on neutrophils, monocytes, and tumor cells. This structure has previously been identified as the ligand for another member of the LEC-CAM family of cell adhesion molecules, endothelial cell-leukocyte adhesion molecule 1, which also binds neutrophils and monocytes. The results demonstrate that although the two LEC-CAMs differ in their biological activities by their distribution and mode of expression, they are capable of mediating cell adhesion by recognition of the same carbohydrate ligand.
Polley
M J
MJ
Cytel Corp., La Jolla, CA 92037.
Phillips
M L
ML
Wayner
E
E
Nudelman
E
E
Singhal
A K
AK
Hakomori
S
S
Paulson
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology / Q000502:physiology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000276:immunology / Q000502:physiology*; D006801:Humans; D016256:Lewis X Antigen / Q000276:immunology*; D008024:Ligands; D008081:Liposomes; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D019007:P-Selectin; D015539:Platelet Activation; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D011971:Receptors, Immunologic / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016256:Lewis X Antigen; D008024:Ligands; D008081:Liposomes; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D011971:Receptors, Immunologic