The presence of free D-serine in rat brain.
Free amino acid enantiomers in adult rat brain extracts were analyzed as their N,O-pentafluoropropionyl isopropyl derivatives by gas chromatography on a capillary column of Chirasil-L-Val. A peak X, which exhibited the same retention time as the N,O-pentafluoropropionyl isopropyl derivative of authentic D-serine, was detected in the brain extracts. Electron impact and positive chemical ionization mass spectra of the peak X of the brain extracts were identical to those of authentic D-serine. The concentration of free D-serine and the ratio of D-serine/total serine in the brain were estimated to be 0.27 and 0.23 mumol/g of wet weight, respectively. These data provide the first evidence that substantial quantities of free D-serine are present in mammalian brain tissues.
Hashimoto
A
A
Division of Mental Disorder Research, National Center of Neurology and Psychiatry, Tokyo, Japan.
Nishikawa
T
T
Hayashi
T
T
Fujii
N
N
Harada
K
K
Oka
T
T
Takahashi
K
K
D000818:Animals; D001923:Brain Chemistry; D002849:Chromatography, Gas; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012694:Serine / Q000032:analysis*; D013237:Stereoisomerism
D012694:Serine