Design of a gas chromatograph with parallel radioactivity and mass spectrometric detection. Application to the identification of the major metabolite of d-limonene associated with alpha 2u-globulin.
A Perkin Elmer 3920 gas chromatograph, equipped with a versatile inlet system (i.e. an injector/trap), was interfaced to a radioactivity detector and a mass-selective detector (H/P 5970B) to identify 14C-labeled compounds. The use of a pre-trap as a demountable, programmable-temperature injector, in conjunction with the injector/trap, allowed the introduction of 0.5-ml samples of rat kidney cytosol extracts to 0.32 mm I.D. capillary columns. The instrumentation greatly facilitated the identification of the major radiolabeled metabolite of d-limonene associated with the male rat-specific protein alpha 2u-globulin as 1,2-cis-d-limonene oxide.
Rodriguez
P A
PA
Procter and Gamble Co., Miami Valley Labs., Cincinnati, OH 45239-8707.
Takigiku
R
R
Lehman-McKeeman
L D
LD
Fey
M L
ML
Eddy
C L
CL
Caudill
D
D
D000284:Administration, Oral; D000818:Animals; D053138:Cyclohexenes; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D000077222:Limonene; D008297:Male; D051381:Rats; D013729:Terpenes / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism*
D053138:Cyclohexenes; D013729:Terpenes; D000511:alpha-Macroglobulins; D000077222:Limonene