title: Synthetic oligonucleotides for biomedical applications.
abstract: New developments of oligonucleotides for biomedical applications are surveyed. Diagnostic probes were conveniently labelled by enzymatic immunogenic tailing with 5-bromo-deoxyuridine triphosphate. "Antisense" oligonucleotides of potential therapeutic value were stabilized against nucleolytic decay by inversion of terminal internucleotidic linkages. Introduction of 2'-deoxy-2'-fluoro-nucleotide units enhances duplex stability and conveys resistance to ribonucleases.
authors.affiliation: Universität Ulm, Sektion Polymere, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Seliger
authors.forename: A
authors.initials: A
authors.lastname: Fröhlich
authors.forename: G
authors.initials: G
authors.lastname: Gröger
authors.forename: B
authors.initials: B
authors.lastname: Krist
authors.forename: M
authors.initials: M
authors.lastname: Montenarh
authors.forename: H
authors.initials: H
authors.lastname: Rösch
authors.forename: R
authors.initials: R
authors.lastname: Rösch
authors.forename: F R
authors.initials: FR
authors.lastname: Ortigao
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D005461:Fluorine; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis; D009843:Oligoribonucleotides / Q000138:chemical synthesis; D012313:RNA / Q000378:metabolism; D012107:Research Design; D012260:Ribonucleases / Q000378:metabolism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D009843:Oligoribonucleotides; D005461:Fluorine; D012313:RNA; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases; D004262:DNA Restriction Enzymes