Development of an immunoenzymometric assay for alpha 1-microglobulin and measurement of its serum concentration in normal and HIV-infected persons.
alpha 1-Microglobulin was purified from urine to a purity of 97.7% in a yield of 25.8%, and was used to produce antibodies in sheep. These antibodies, purified by affinity chromatography, were used to develop a rapid one-step and a two-step immunoenzymometric assay (IEMA). The equilibrium in the reaction between solid phase-adsorbed antibodies and antigen and between the antigen and enzyme-labelled antibodies was attained within 30 and 100 min, respectively. The one-step IEMA permits a good differentiation of low alpha 1-microglobulin concentrations after 30 min reaction time. Its detection limit is 0.35 micrograms/l, and its measurement range is between 0.5 and 100 micrograms/l. The IEMA correlates well with radial immunodiffusion (r = 0.973). The mean alpha 1-microglobulin serum concentration in women is insignificantly lower (33.2 mg/l) than in men (36.1 mg/l). In both sexes the alpha 1-microglobulin concentration increases with age. HIV-infected symptomless men have a significantly lower (15.9 mg/l) alpha 1-microglobulin concentration in serum than normal persons, whereas in AIDS patients it is significantly higher (45.5 mg/l).
Porstmann
T
T
Department of Medical Immunology, Medical School (Charité), Humboldt University Berlin, GDR.
Schmechta
H
H
Hentschel
C
C
Doepel
H
H
Pas
P
P
Becker
J
J
Pergande
M
M
Jung
K
K
Nugel
E
E
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood*; D000328:Adult; D000510:Alpha-Globulins / Q000032:analysis* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D015658:HIV Infections / Q000097:blood*; D015497:HIV-1; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D011480:Protease Inhibitors / Q000097:blood*; D012016:Reference Values; D012044:Regression Analysis
D000510:Alpha-Globulins; D011480:Protease Inhibitors; C016001:alpha-1-microglobulin