title: Comparison of monoclonal antibody and calcofluor white stains for the detection of Pneumocystis carinii from respiratory specimens.
abstract: Three monoclonal antibody staining kits, from Genetic Systems, Disease Detection International, and Meridian Diagnostics, were compared with calcofluor white for the direct detection of Pneumocystis carinii in respiratory specimens. Of the 150 specimens tested, 23 were found positive for P. carinii by any of the four stains; 13 were bronchoalveolar lavage, 7 were induced sputum and 3 were expectorated sputum specimens. All stains detected the positive bronchoalveolar lavage specimens, the Genetic Systems stain detected six induced sputum specimens, and the other stains each detected four induced sputum specimens. The monoclonal antibody stains detected all three expectorated specimens, while the calcofluor stain detected only one. Overall, the sensitivities of the stains were 96% for Genetic Systems, 87% for both Disease Detection International and Meridian Diagnostics, and 78% for calcofluor.
authors.affiliation: Department of Pathology, University of California, Irvine, Medical Center, Orange 92668.
authors.forename: N
authors.initials: N
authors.lastname: Stratton
authors.forename: J
authors.initials: J
authors.lastname: Hryniewicki
authors.forename: S L
authors.initials: SL
authors.lastname: Aarnaes
authors.forename: G
authors.initials: G
authors.lastname: Tan
authors.forename: L M
authors.initials: LM
authors.lastname: de la Maza
authors.forename: E M
authors.initials: EM
authors.lastname: Peterson
mesh_terms: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D001557:Benzenesulfonates; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D005069:Evaluation Studies as Topic; D006801:Humans; D011010:Pneumocystis / Q000276:immunology / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D001557:Benzenesulfonates; C007061:C.I. Fluorescent Brightening Agent 28