Standardization of the Feulgen reaction for absorption DNA image cytometry: a review.
The present paper gives a review of the current potentials and problems of a standardized Feulgen reaction for absorption DNA image cytometry. The cytochemical basis of the Feulgen reaction is described in the first part of this review. Subsequently, several preparatory factors which influence the performance of the Feulgen reaction, such as fixation, acid hydrolysis, composition of the Feulgen reagent and, in histology, embedding, are discussed in more detail. Some user-oriented recommendations for a standard Feulgen technique are given.
Schulte
E K
EK
Anatomische Anstalt, Ludwig-Maximilians-Universität, München, FRG.
D000447:Aldehydes; D002843:Chromatin / Q000648:ultrastructure; D004396:Coloring Agents / Q000737:chemistry / Q000592:standards*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D003720:Densitometry / Q000379:methods*; D005404:Fixatives / Q000494:pharmacology; D006652:Histological Techniques; D006868:Hydrolysis; D012015:Reference Standards; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000592:standards; D013438:Sulfhydryl Compounds / Q000737:chemistry; D009626:Terminology as Topic
D000447:Aldehydes; D002843:Chromatin; D004396:Coloring Agents; C007722:Feulgen stain; D005404:Fixatives; D012394:Rosaniline Dyes; D013438:Sulfhydryl Compounds; D004247:DNA; C005732:thioacetic acid