Expression of mRNA for Gs alpha and Gi2 alpha in primary cultured mouse cerebral cortical neurons.
Developmental changes of mRNAs for alpha-subunits of GTP binding protein (G-protein) such as Gs alpha and Gi2 alpha during neuronal development were examined in both primary cultured cerebral cortical neurons and cerebral cortices obtained from age-matched mice. The expression of mRNAs for both G-protein alpha-subunits in primary cultured neurons showed a development pattern similar to that in the cerebral cortex in vivo. Both mRNAs were expressed at an early stage of neuronal development, and the expression patterns of mRNA for both G-protein alpha-subunits were found to be only slightly changed during development. These results suggest that the G-protein appears at an early stage of neuronal development and may play an important role therein.
Hirouchi
M
M
Department of Pharmacology, Kyoto Prefectural University of Medicine, Japan.
Ohkuma
S
S
Kuriyama
K
K
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012313:RNA; D012333:RNA, Messenger / Q000378:metabolism*
D012333:RNA, Messenger; D012313:RNA; D019204:GTP-Binding Proteins