Rat vasopressin and oxytocin genes are linked by a long interspersed repeated DNA element (LINE): sequence and transcriptional analysis of LINE.
Sequence analysis of the rat vasopressin and oxytocin gene family reveals that the two genes are linked by a long interspersed repeated DNA element (LINE) giving rise to seven long open reading frames encoding hypothetical proteins of 99 to 556 amino acid residues. Furthermore, although both DNA strands of LINEs serve as templates for transcription, transcripts initiated at the 3' end are more abundant than those started from the 5' end. The LINEs are transcribed preferentially in brain tissues as analyzed by Northern blot, in situ hybridization, and RNase protection experiments. The data show that most LINEs are transcribed at their entire length and that a major fraction of respective RNAs does not enter the cytoplasm but remains in the cell nucleus.
Schmitz
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Institut für Zellbiochemie und klinische Neurobiologie, UKE, Universität Hamburg, FRG.
Mohr
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Richter
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D004247:DNA / Q000737:chemistry; D008040:Genetic Linkage; D007031:Hypothalamus / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D010121:Oxytocin / Q000235:genetics*; D010902:Pituitary Gland / Q000378:metabolism; D012313:RNA / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014667:Vasopressins / Q000235:genetics*
D014667:Vasopressins; D010121:Oxytocin; D012313:RNA; D004247:DNA