Non-radioactive restriction fragment length polymorphism (RFLP) typing of Clostridium difficile.
A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemiluminescence (ECL) gene detection system. The probe labelling procedure was easy to perform, taking only 20 min. The complete typing method was comparatively simple, reproducible and readily adaptable to most bacterial genera.
Bowman
R A
RA
Department of Clinical Microbiology, University of Western Australia, Nedlands.
O'Neill
G L
GL
Riley
T V
TV
D001315:Australia; D015373:Bacterial Typing Techniques; D015139:Blotting, Southern; D016360:Clostridioides difficile / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D006801:Humans; D008163:Luminescent Measurements; D012150:Polymorphism, Restriction Fragment Length; D015347:RNA Probes / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics
D015347:RNA Probes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal