Neurofilament M and calbindin D28k are present in mutually exclusive subpopulations of enteric neurons in the rat submucous plexus.
Immunocytochemical methods have been used to examine the localisation of 3 neurofilament proteins and the calcium binding protein, calbindin D28k, in whole mount preparations of the submucous plexus in the Wistar rat. Neurofilament-M (160 kDA protein) was present in 40% of the submucosal neurons, staining fine filaments in the soma and the axonal processes. Calbindin D28k was present in 40% of the submucosal neurons staining both the soma and nerves within the plexus. The neurofilament proteins and calbindin D28k were never observed within the same neurons. Neurofilament-M was co-localised with substance P and calcitonin gene-related peptide but not somatostatin or the other neuropeptides investigated. Calbindin D28k was co-localised with vasoactive intestinal polypeptide and neuropeptide Y. Galanin- and somatostatin-immunoreactive neurons did not contain either the neurofilament proteins or calbindin D28k. The results demonstrate the presence of subsets of submucosal neurons that can be distinguished by the presence of neurofilament-M or calbindin D28k.
Buchan
A M
AM
Department of Physiology, University of British Columbia, Vancouver, Canada.
D000818:Animals; D000911:Antibodies, Monoclonal; D064092:Calbindin 1; D064026:Calbindins; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis*; D007413:Intestinal Mucosa / Q000294:innervation*; D007421:Intestine, Small / Q000294:innervation; D009197:Myenteric Plexus / Q000166:cytology*; D016900:Neurofilament Proteins; D009474:Neurons / Q000166:cytology*; D009478:Neuropeptide Y / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D013373:Substance P / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D000911:Antibodies, Monoclonal; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D009478:Neuropeptide Y; D064030:S100 Calcium Binding Protein G; C061195:neurofilament protein M; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D015740:Calcitonin Gene-Related Peptide