Staining plant cells with silver. I. The salt-nylon technique.
A technique is described for selectively silver staining nucleoli, active nucleolus organizers, nucleolar material attached to chromosomes, kinetochores, synaptonemal complexes, and chromosome cores in plant cells. The technique, called salt-nylon silver staining, involves spreading cells on glass slides, treating the cells with a solution of saline sodium citrate, and incubating the cells in a silver nitrate solution covered with nylon screen. Selected variables important for achieving reliable silver staining are considered.
Stack
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Department of Biology, Colorado State University, Fort Collins 80523.
Herickhoff
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Sherman
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Anderson
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D000490:Allium / Q000166:cytology; D000970:Antineoplastic Agents; D002466:Cell Nucleolus / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure; D008853:Microscopy; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D009757:Nylons; D059828:Plant Cells; D012834:Silver; D012835:Silver Nitrate; D013194:Staining and Labeling; D013573:Synaptonemal Complex
D000970:Antineoplastic Agents; D009757:Nylons; D012834:Silver; D012835:Silver Nitrate