In vivo neutralization of duck hepatitis B virus by antibodies specific to the N-terminal portion of pre-S protein.
The neutralization of duck hepatitis B virus (DHBV) infection using antibodies directed against the N-terminal portion of the large surface protein was examined in vitro and in vivo. We demonstrate here that a monoclonal antibody, directed against an epitope mapped between aa 77 and aa 100 on the DHBV pre-S, exerts a similar neutralizing activity (77%) both in vivo and in vitro. Furthermore, we have found that a polyclonal antiserum raised against the bacterially expressed 131 first amino acids of the DHBV pre-S region abolished the infectivity of DHBV in ducklings. Therefore, antibodies against a peptide representing most of the DHBV pre-S region (1-131) or a monoclonal antibody specific to an epitope within this region neutralizes in vivo DHBV infectivity.
Lambert
V
V
INSERM U 271, Lyon, France.
Chassot
S
S
Kay
A
A
Trepo
C
C
Cova
L
L
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology*; D004279:DNA, Viral / Q000302:isolation & purification; D004372:Ducks; D000939:Epitopes / Q000032:analysis*; D015611:Hepatitis B Virus, Duck / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D008099:Liver / Q000382:microbiology; D009500:Neutralization Tests; D011498:Protein Precursors / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D004279:DNA, Viral; D000939:Epitopes; D011498:Protein Precursors; D014759:Viral Envelope Proteins