Ecdysterone decreases the transcription level of the retrotransposons 1731 and 412 in a Drosophila cell line.
Cultured Drosophila melanogaster cells are responsive to the steroid hormone 20-hydroxyecdysone (ecdysterone). The transcripts of two copia-like transposable elements, 412 and 1731 were examined in cells treated for different lengths of time by the hormone. Using dot-blot and Northern-blot analysis, we have shown that the transcripts of these two retrotransposons were rapidly decreased by ecdysterone treatment.
Becker
J
J
U-A CNRS 1135, Université P. et M. Curie, Paris, France.
Micard
D
D
Becker
J L
JL
Fourcade-Peronnet
F
F
Dastugue
B
B
Best-Belpomme
M
M
D000818:Animals; D002460:Cell Line / Q000187:drug effects; D004251:DNA Transposable Elements / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000235:genetics*; D004441:Ecdysterone / Q000494:pharmacology*; D012313:RNA / Q000032:analysis; D014158:Transcription, Genetic / Q000187:drug effects*
D004251:DNA Transposable Elements; D004441:Ecdysterone; D012313:RNA