Inhibition of HIV replication in lymphocyte cultures of virus-positive subjects in the presence of sho-saiko-to, an oriental plant extract.
An oriental remedy, Sho-saiko-to (SST) consisting of a mixture of aqueous extracts from seven different plants and whose most active component is the chemically defined compound baicalein was tested for its ability to inhibit the production of the human immunodeficiency virus (HIV). The testing was done with cultures of human lymphocytes obtained from HIV-positive asymptomatic subjects and patients with ARC or AIDS. The replication of the virus was monitored by quantitative assay of the reverse transcriptase (RT) activity and of the synthesis of antigen p24. The lymphocyte cultures (LC) were maintained in the absence and in the presence of 25, 50 or 100 micrograms/ml of SST, and monitored for up to 5 weeks. The results showed that in LC from asymptomatic subjects RT activity and synthesis of p24 was completely inhibited by low concentrations of SST. High concentrations of SST inhibited virus replication in 80% of LC from ARC patients, but were completely ineffective in LC from AIDS patients. It was observed that the RT activity was more sensitive to inhibition by SST than the synthesis of p24, and that the antiviral effect was dependent on the virus load of the LC.
Buimovici-Klein
E
E
Department of Pediatrics, St. Luke's Roosevelt Hospital Center, New York, NY 10019.
Mohan
V
V
Lange
M
M
Fenamore
E
E
Inada
Y
Y
Cooper
L Z
LZ
D000386:AIDS-Related Complex / Q000201:enzymology; D000163:Acquired Immunodeficiency Syndrome / Q000201:enzymology; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D015331:Cohort Studies; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D015683:Gene Products, gag / Q000276:immunology; D006678:HIV / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D015483:HIV Antibodies / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology; D016655:HIV Core Protein p24; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000382:microbiology*; D008297:Male; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012680:Sensitivity and Specificity; D014758:Viral Core Proteins / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D015683:Gene Products, gag; D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D014758:Viral Core Proteins; C018142:saiko-keishi-to; D012194:RNA-Directed DNA Polymerase