Induction of carbonic anhydrase I isozyme precedes the globin synthesis during erythropoiesis in K562 cells.
Induction of carbonic anhydrase isozyme I (CA-I) by erythropoietin or hemin was investigated using erythroleukemia (K562) cells. Immunological estimation and purification of carbonic anhydrases showed that untreated K562 cells contained only carbonic anhydrase isozyme II(CA-II), while incubation of the cells with 2 units of erythropoietin (EP) per ml of the incubation medium or with 50 microM hemin resulted in the induction of CA-I. The purified CA-I induced in K562 cells was enzymatically and immunologically identical to that from mature erythrocytes. Flow cytometric analysis showed that incubation of K562 cells with EP as well as hemin induced CA-I at the 3rd h, while alpha-globin was detected at the 8th h. Northern blot analysis of CA-I mRNA using a cloned genomic DNA as a probe showed that mRNA of CA-I was induced by EP. These results suggest that induction of CA-I is regulated at the transcriptional level during developmental changes of erythroid cells, and that CA-I may play a physiologically important role during erythroid differentiation.
Kondo
T
T
First Department of Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.
Sakai
M
M
Isobe
H
H
Taniguchi
N
N
Nishi
S
S
Kawakami
Y
Y
D015152:Blotting, Northern; D015153:Blotting, Western; D002256:Carbonic Anhydrases / Q000378:metabolism*; D004790:Enzyme Induction; D004920:Erythropoiesis / Q000502:physiology*; D004921:Erythropoietin / Q000494:pharmacology; D005914:Globins / Q000096:biosynthesis*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000097:blood / Q000378:metabolism*; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured
D004921:Erythropoietin; D005914:Globins; D002256:Carbonic Anhydrases