title: A lymphocyte homing receptor (L-selectin) mediates the in vitro attachment of lymphocytes to myelinated tracts of the central nervous system.
abstract: Lymphocytes enter lymph nodes by first adhering to high endothelial venules, an adhesive event mediated by a lectinlike lymphocyte receptor (L-selectin). Previously, it was shown with an in vitro assay that lymphocytes preferentially adhere to myelin-rich regions in brain sections. Here, using a recombinant form of L-selectin as an immunohistochemical reagent, we demonstrate potential ligands for L-selectin in myelinated regions of the central but not the peripheral nervous system. Using several antibodies and phorbol ester downmodulation of the receptor, we establish that L-selectin on human lymphocytes has a primary involvement in lymphocyte adherence to the myelinated regions. On mouse lymphocytes, the contribution of L-selectin appears to be partial. These findings raise the possibility that leukocyte targeting to myelin-rich regions, via a L-selectin dependent mechanism, may be a factor in the pathogenesis of certain central nervous system demyelinating diseases.
authors.affiliation: Department of Anatomy, University of California, San Francisco 94143-0452.
authors.forename: K
authors.initials: K
authors.lastname: Huang
authors.forename: J S
authors.initials: JS
authors.lastname: Geoffroy
authors.forename: M S
authors.initials: MS
authors.lastname: Singer
authors.forename: S D
authors.initials: SD
authors.lastname: Rosen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002118:Calcium / Q000502:physiology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology*; D003711:Demyelinating Diseases / Q000209:etiology; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D009186:Myelin Sheath / Q000502:physiology*; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D002118:Calcium