title: Isoenzyme analysis of Pseudomonas cepacia as an epidemiologic tool.
abstract: Multilocus enzyme electrophoresis has successfully been used to establish basic marker systems for the epidemiologic analysis of a variety of bacterial pathogens. This study was done to determine the efficacy of this technique for characterizing Pseudomonas cepacia, using 31 known-related strains isolated during an outbreak of infections involving intrinsically contaminated povidone-iodine solution, and five outbreak-unrelated strains used in serotyping of P. cepacia. Crude cell extracts were analyzed by starch gel electrophoresis for electrophoretic variants using 13 enzyme substrates; esterase bands were detected using an additional four substrates. The 31 outbreak strains had identical isoenzyme patterns for all enzymes examined. Five electrophoretic types were obtained for the serotyping strains; electrophoretic mobilities of one of the five strains corresponded to the patterns obtained for the outbreak strains. These results suggest that enzyme electrophoretic typing may be a useful adjunct to other typing methods used in epidemiologic analyses of P. cepacia infections.
authors.affiliation: Nosocomial Infections Laboratory Branch, Disease Control, Atlanta, Georgia 30333.
authors.forename: L A
authors.initials: LA
authors.lastname: Carson
authors.forename: R L
authors.initials: RL
authors.lastname: Anderson
authors.forename: A L
authors.initials: AL
authors.lastname: Panlilio
authors.forename: C M
authors.initials: CM
authors.lastname: Beck-Sague
authors.forename: J M
authors.initials: JM
authors.lastname: Miller
mesh_terms: D016956:Burkholderia cepacia / Q000145:classification* / Q000201:enzymology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D004196:Disease Outbreaks; D004592:Electrophoresis, Starch Gel; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology*; D012703:Serotyping
chemical_list: D007527:Isoenzymes