title: Characterization of Sendai virus-induced human placental trophoblast interferons.
abstract: Human placental trophoblast cultures produce a mixture of interferons (IFNs) when challenged with Sendai virus. High-performance dye-ligand and immunoaffinity chromatography of a trophoblast IFN (tro-IFN) preparation enabled the isolation of three antigenically distinct IFNs, alpha I, alpha II 1 and beta, with Mrs of 16K, 22K and 24K respectively, by reducing and non-reducing SDS-PAGE. The major IFN, responsible for 75% of the total antiviral activity, was tro-IFN-beta, with the remaining activity being due to tro-IFN-alpha I and tro-IFN-alpha II 1, as determined by an antiviral neutralization test using specific anti-human IFN antibodies. The antiviral activities of the tro-IFNs were stable at pH 2.0 for 24 h and tro-IFN-alpha II 1 and -beta were shown to be glycoproteins. The three tro-IFNs showed different antiviral activities when assayed on human and bovine cell species; tro-IFN-alpha I and alpha II 1 protected both human and bovine (MDBK) cells from virus infection, whereas tro-IFN-beta showed a high degree of species specificity, protecting only the human cell types tested.
authors.affiliation: Danish Cancer Society, Department of Virus and Cancer, Aarhus C.
authors.forename: G
authors.initials: G
authors.lastname: Aboagye-Mathiesen
authors.forename: F D
authors.initials: FD
authors.lastname: Tóth
authors.forename: C
authors.initials: C
authors.lastname: Juhl
authors.forename: N
authors.initials: N
authors.lastname: Nørskov-Lauritsen
authors.forename: P M
authors.initials: PM
authors.lastname: Petersen
authors.forename: V
authors.initials: V
authors.lastname: Zachar
authors.forename: P
authors.initials: P
authors.lastname: Ebbesen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002417:Cattle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007372:Interferons / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D009500:Neutralization Tests; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D014327:Trophoblasts / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007372:Interferons