Analysis of CD34-positive hemopoietic progenitor cells from normal human adult peripheral blood: flow-cytometrical studies and in-vitro colony (CFU-GM, BFU-E) assays.
Hemopoietic progenitor cells are present in minute numbers in the peripheral blood of healthy adults. By in vitro colony-assays evaluating BFU-E- and CFU-GM-growth, their numbers have been estimated to be about 1,000 per 1.0 ml of whole blood. Employing a CD34-moAb, detecting an antigen present on virtually all hemopoietic progenitor cells, and by using multiparameter flow-cytometry, we have designed a flow-cytometric method for the quantitation of CD34(+)-cells in blood. Comparative studies on bone marrow and blood have shown that CD34(+)-cells from both sources display almost identical light-scatter characteristics. They differ, however, with regard to the coexpression of the CD33- and the CD19-antigens. In vitro colony-assays for BFU-E and CFU-GM in single cultures have shown that about 25% of the CD34(+)-cells from blood were clonogenic in vitro. Our data indicate that the CD34(+)-cells from peripheral blood differ substantially from bone marrow CD34(+)-cells.
Serke
S
S
Universitätsklinikum Rudolf Virchow, Charlottenburg, Federal Republic of Germany.
Säuberlich
S
S
Abe
Y
Y
Huhn
D
D
D000328:Adult; D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D002452:Cell Count; D003114:Colony-Forming Units Assay; D015672:Erythroid Precursor Cells / Q000166:cytology; D005434:Flow Cytometry; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D008264:Macrophages / Q000166:cytology
D015703:Antigens, CD; D018952:Antigens, CD34