title: Glycolipid anchorage of Plasmodium falciparum surface antigens.
abstract: Human red blood cells (RBC) were infected with the malarial parasite Plasmodium falciparum, the anchoring of schizont proteins to RBC membranes by glycoinositol phospholipids was demonstrated by three criteria: (1) metabolic incorporation of 3H-ethanolamine and 3H-myristate into the protein; (2) release of 35S-methionine-labelled protein into the supernatant after incubation with phosphatidylinositol-specific phospholipase C; and (3) the exposure of a glycoinositol phosphate epitope on the methionine-labelled protein following phospholipase C cleavage. Labelled proteins were analysed by immunoprecipitation, polyacrylamide gel electrophoresis in sodium dodecylsulphate and gel fluorography. Several candidate proteins were observed when each criteria was investigated. Among these, 3 proteins which met all three criteria were identified by immunoprecipitation with monospecific sera or monoclonal antibodies. These included 3 possible vaccine candidates, the p190 major surface antigen, the p76 serine protease and the p71 protein which is thought to be a member of the family of heat-shock Hsp70 proteins.
authors.affiliation: Unit of Experimental Parasitology, Institut Pasteur, Paris.
authors.forename: C
authors.initials: C
authors.lastname: Braun Breton
authors.forename: T L
authors.initials: TL
authors.lastname: Rosenberry
authors.forename: L H
authors.initials: LH
authors.lastname: Pereira da Silva
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D004910:Erythrocyte Membrane / Q000276:immunology; D006017:Glycolipids / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*; D011233:Precipitin Tests; D015800:Protozoan Proteins / Q000276:immunology*; D010738:Type C Phospholipases / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D000939:Epitopes; D006017:Glycolipids; D015800:Protozoan Proteins; D010738:Type C Phospholipases