Keratin and carcinoembryonic antigen (CEA) in human melanoma cells.
Human melanomas are known to contain vimentin intermediate filaments but there has been some dispute about their expression of cytokeratins. The cytoplasm of human M21 melanoma cells maintained in culture reacted with a rabbit anti-keratin antibody and two monoclonal anti-keratin antibodies AE1 and AE2. Cells derived directly from subcutaneous xenografts of M21 melanoma in nude mice, however, failed to express cytokeratins. The presence of keratin filaments in cultured M21 cells was confirmed by electronmicroscopic and immuno-electronmicroscopic examinations of cell extracts. Polyacrylamide gel electrophoresis (PAGE), revealed 46 KD keratin proteins in cultured M21 cells. Small amounts of these low molecular weight keratins were detected by PAGE in M21 melanoma xenografts even though immunofluorescence and immunoperoxidase assays failed to demonstrate keratin at the light microscopic level. Immunofluorescence revealed keratin and carcinoembryonic antigen (hitherto undetected in human melanomas) first on the 9th day of culture of xenograft-derived M21 cells. The appearance of keratin and CEA in M21 melanoma cells in vitro was not affected by inhibition of cellular proliferation or as a result of exposure to methotrexate or adriamycin. However, adriamycin altered the cytoplasmic distribution of keratin.
Om
A
A
Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada.
Ghose
T
T
Rowden
G
G
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000302:isolation & purification*; D002457:Cell Extracts; D004317:Doxorubicin / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D005779:Immunodiffusion; D007150:Immunohistochemistry; D007633:Keratins / Q000302:isolation & purification*; D008545:Melanoma / Q000378:metabolism*; D008727:Methotrexate / Q000494:pharmacology; D008854:Microscopy, Electron; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000378:metabolism
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D002457:Cell Extracts; D007633:Keratins; D004317:Doxorubicin; D008727:Methotrexate