Cloning and expression of a rat cardiac delayed rectifier potassium channel.
We have cloned a cDNA (designated RAK) coding for a delayed-rectifier K current (IRAK) from adult rat heart atrium and expressed it in Xenopus oocytes. RAK differs from the cloned rat brain K current, BK2 [McKinnon, D. (1989) J. Biol. Chem. 264, 8230-8236], by one amino acid at residue 411. RAK expressed in oocytes compares closely to the intrinsic adult rat atrial delayed-rectifier current measured by using whole-cell recording of single isolated cells. Northern blot analysis confirmed the presence of the channel in adult rat atrium, and to a lesser extent, in rat ventricle. IRAK activates with time constants ranging from 58 ms at -20 mV to 6 ms at +60 mV and does not show significant inactivation over 800 ms. It is blocked by 4-aminopyridine greater than barium much greater than tetraethylammonium chloride, which is similar to the relative potencies of these blockers on the native delayed rectifier current. We conclude that the main delayed rectifier K current in adult rat atria is virtually identical to a neuronal delayed rectifier, BK2.
Paulmichl
M
M
Department of Pharmacology, Mayo Foundation, Rochester, MN 55905.
Nasmith
P
P
Hellmiss
R
R
Reed
K
K
Boyle
W A
WA
Nerbonne
J M
JM
Peralta
E G
EG
Clapham
D E
DE
D015761:4-Aminopyridine / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D016275:Atrial Function; D001464:Barium / Q000494:pharmacology; D003001:Cloning, Molecular; D015870:Gene Expression; D005914:Globins / Q000235:genetics; D006321:Heart / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D011061:Poly A / Q000235:genetics; D011188:Potassium / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D014982:Xenopus laevis
D015221:Potassium Channels; D012333:RNA, Messenger; D013757:Tetraethylammonium Compounds; D011061:Poly A; D001464:Barium; D012313:RNA; D019789:Tetraethylammonium; D005914:Globins; D015761:4-Aminopyridine; D011188:Potassium