[Effects of lymphokines on DNA structure of in vitro cultured human lymphocytes. Connection with cAMP and oligoadenylate synthetase systems].
We investigated the influence of recombinant interferons (INF) alpha 2 and gamma and interleukin (IL) 2 and natural purified IL 1 on the activity of oligoadenylate synthetase proliferation level and the DNA structure of cultured in vitro human peripheral blood lymphocytes. It was shown that the proliferation of mitogen-stimulated lymphocytes increased in the presence of IL 1, IL 2 and INF gamma, but there was no proliferation in the presence of INF alpha 2. Oligoadenylate synthetase activity was increased after 18 hours incubation in the presence of all these lymphokines, but after 48 and 72 hours it was increased only in the presence of INF alpha 2 or ConA or INF alpha 2 with ConA together. INF alpha 2, INF gamma and IL 1 stabilized the DNA structure of intact and mitogen-stimulated lymphocytes. cAMP and oligoA synthetase are the specific second messengers of the interferon system and they stabilized the lymphocytes DNA structure too. Cultivation of lymphocytes in the presence of RNA and protein biosynthesis inhibitors--actinomycin D and cycloheximide was followed by accumulation of alkali-labile sites in their DNA. That means that some short lived proteins are needed for the stabilization of native DNA structure.
Moskaleva
E Iu
EIu
D015088:2',5'-Oligoadenylate Synthetase / Q000097:blood*; D002455:Cell Division; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000097:blood*; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry*; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008222:Lymphokines / Q000494:pharmacology*
D007378:Interleukins; D008222:Lymphokines; D004247:DNA; D007372:Interferons; D000242:Cyclic AMP; D015088:2',5'-Oligoadenylate Synthetase
Vliianie limfokinov na strukturu DNK kul'tiviruemykh in vitro limfotsitov cheloveka, sviaz' s sistemoĭ tsAMF i oligoA.