Identification of type-specific linear epitopes in the glycoproteins gp46 and gp21 of human T-cell leukemia viruses type I and type II using synthetic peptides.
Synthetic peptides of 20-25 amino acids were employed in enzyme-linked immunosorbent assays to identify linear epitopes in the external glycoprotein gp46 and the transmembrane glycoprotein gp21 of human T-cell leukemia/lymphotropic viruses type I (HTLV-I) and II (HTLV-II). Ten linear epitopes were identified in the HTLV-I glycoproteins, seven in gp46 and three in gp21. Three major linear epitopes were identified in the gp46 of HTLV-II. Peptides representing linear epitopes of gp46 were found to be sensitive and specific for the detection of antibody and permit serological identification and differentiation of HTLV-I and HTLV-II infections.
Horal
P
P
Department of Clinical Virology, University of Göteborg, Sweden.
Hall
W W
WW
Svennerholm
B
B
Lycke
J
J
Jeansson
S
S
Rymo
L
L
Kaplan
M H
MH
Vahlne
A
A
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D015480:Deltaretrovirus Antibodies / Q000276:immunology; D015485:Deltaretrovirus Antigens / Q000276:immunology*; D000939:Epitopes; D015686:Gene Products, env / Q000276:immunology*; D015490:HTLV-I Infections / Q000276:immunology; D015491:HTLV-II Infections / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D015367:Human T-lymphotropic virus 2 / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology*; D013045:Species Specificity; D054299:env Gene Products, Human Immunodeficiency Virus
D015480:Deltaretrovirus Antibodies; D015485:Deltaretrovirus Antigens; D000939:Epitopes; D015686:Gene Products, env; D015682:Retroviridae Proteins, Oncogenic; D054299:env Gene Products, Human Immunodeficiency Virus; C055265:gp21 protein, Human T-lymphotropic virus 1; C055266:gp46 protein, Human T-cell leukemia virus type I; C069568:gp46 protein, Human immunodeficiency virus 2; C069569:human T-cell leukemia virus type-II protein gp21