Development of a radioimmunoassay for human group-II phospholipase A2 and demonstration of postoperative elevation.
A radioimmunoassay (RIA) for the determination of human group-II phospholipase A2 (M-PLA2) has been developed. M-PLA2 was purified from human spleen. Monoclonal antibody (IgG) was prepared by fusion of splenic cells from immunized mice with M-PLA2 and the mouse myeloma cell line NS-1. The RIA was carried out by a single antibody method. The assay is sensitive (0.78 micrograms/l), reproducible and specific. In healthy individuals, the serum M-PLA2 concentration ranges from 1.4 to 4.2 micrograms/l, the average being 2.2 +/- 0.1 micrograms/l (mean +/- SE). Using the RIA, we found increased serum M-PLA2 in patients with various infections and malignant tumors. We also showed the postoperative transient elevation of serum M-PLA2 in cases without any infectious complications. The elevation was independent of the surgical procedure or site. The maximum serum M-PLA2 level was seen on the 2nd to 4th postoperative day. In these patients, the serum M-PLA2 and C-reactive protein levels were significantly correlated. The present study indicated that serum M-PLA2 is an acute phase reactant.
Matsuda
Y
Y
Second Department of Surgery, Osaka University Medical School, Japan.
Ogawa
M
M
Sakamoto
K
K
Yamashita
S
S
Kanda
A
A
Kohno
M
M
Yoshida
N
N
Nishijima
J
J
Murata
A
A
Mori
T
T
D000209:Acute-Phase Proteins / Q000737:chemistry; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D010741:Phospholipases A / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D054467:Phospholipases A2; D011184:Postoperative Period; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards; D012016:Reference Values; D015203:Reproducibility of Results; D013154:Spleen / Q000201:enzymology*
D000209:Acute-Phase Proteins; D007527:Isoenzymes; D010741:Phospholipases A; D054467:Phospholipases A2