[Serologic characterization of granulocyte-specific antibodies in neonatal alloimmune neutropenias].
Serological properties of nine granulocyte specific alloantibodies in maternal sera and sera of the newborns were investigated by granulocyte immunofluorescence, granulocyte agglutination and granulocyte cytotoxicity. In the immunofluorescence assay the reactions were more prominent than in the agglutination assay. Only one antibody yielded a positive reaction in the cytotoxicity assay. Four NA2-, two NA1- and one NB1-antibody could be identified. The immunofluorescence assay showed a mixed pattern with the NB1-antibody, indicating a heterogenous distribution of the NB1-antigen on granulocytes of some donors. In titration studies with heterozygous and homozygous donors a gene-doses-effect was demonstrable.
Bux
J
J
Institut für Klinische Immunologie und Transfusionsmedizin, Justus-Liebig-Universität, Giessen.
Kissel
K
K
Neppert
J
J
Mueller-Eckhardt
C
C
D000372:Agglutination Tests; D001782:Blood Donors; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D005787:Gene Frequency / Q000235:genetics; D006098:Granulocytes; D006801:Humans; D007231:Infant, Newborn; D007518:Isoantibodies / Q000032:analysis* / Q000235:genetics; D007519:Isoantigens / Q000235:genetics / Q000276:immunology*; D009503:Neutropenia / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D010641:Phenotype
D007518:Isoantibodies; D007519:Isoantigens
Serologische Charakterisierung von granulozytenspezifischen Antikörpern bei neonatalen Alloimmunneutropenien.