Modifying effects of interferon and puromycin on cytogenetic damage induced by alkylating drug phopurine in human lymphocytes.
The induction of sister chromatid exchanges (SCEs) by the bifunctional alkylating antineoplastic drug phopurine (2-dimethyl-amino-6-diethyleneiminophosphamido-7-methylpurine) and its modification by human recombinant interferons alpha 2, beta and gamma (HuIFN alpha 2, HuIFN beta and HuIFN gamma) and puromycin (PM) were studied in human lymphocytes. Results demonstrated a striking similarity in the modifying action of HuIFN alpha 2 and PM: 1) both modifiers reduced SCE values induced by phopurine, 2) at high and low doses of phopurine the effect of both modifiers was minimal, and 3) both agents were able to convert DNA lesions from short-term to long-term.
Lazutka
J R
JR
Ecological Genetics Laboratory, Vilnius University, Lithuania, USSR.
Jarmalaitè
S
S
Lekevicius
R
R
D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology; D001388:Aziridines; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004347:Drug Interactions; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007372:Interferons / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology*; D011691:Puromycin / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects
D000970:Antineoplastic Agents; D001388:Aziridines; D009943:Organophosphorus Compounds; D011687:Purines; D011691:Puromycin; C015893:pumitepa; D007372:Interferons