The identification of the beta 2-microglobulin binding antigen encoded by the human CD1D gene.
Human cluster of differentiation (CD1) is a family of cell surface glycoproteins composed of a 43-49-kDa heavy chain non-covalently associated with beta 2-microglobulin. Five human CD1 genes have been detected and cloned. Three genes (CD1A, -B and -C) encode the serologically defined CD1a, -b and -c antigens. Thus two genes remain, CD1D and CD1E, whose protein products have not been characterized so far. This report describes how a beta-galactosidase-CD1D fusion protein was used to raise specific antisera and a monoclonal antibody against the CD1D gene product. The monoclonal antibody defines a cell surface molecule expressed on a cortical thymocyte cell line and is composed of a 49-kDa heavy chain associated with beta 2-microglobulin, which is serologically distinct from CD1a.
Bilsland
C A
CA
Medical Research Council Laboratory of Molecular Biology, Cambridge.
Milstein
C
C
D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D018949:Antigens, CD1; D000943:Antigens, Differentiation / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006801:Humans; D008970:Molecular Weight; D011233:Precipitin Tests; D011993:Recombinant Fusion Proteins / Q000276:immunology; D001613:beta 2-Microglobulin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018949:Antigens, CD1; D000943:Antigens, Differentiation; D011993:Recombinant Fusion Proteins; D001613:beta 2-Microglobulin