Eosinophils from patients with blood eosinophilia express transforming growth factor beta 1.
The infiltration of eosinophils into tissues during pathologic responses is often associated with extracellular matrix alterations such as fibrosis. Transforming growth factor-beta 1 (TGF-beta 1) is a well-characterized multifunctional cytokine known to exert potent effects on the extracellular matrix. In this report, we showed the production of TGF-beta 1 by human eosinophils from patients with blood eosinophilia. Northern blot analysis using RNA isolated from eosinophils purified from a patient with the idiopathic hypereosinophilic syndrome (HES) detected the 2.5-kb TGF-beta 1 transcript. In situ hybridization and immunohistochemistry of leukocytes from two patients with HES and two patients with blood eosinophilia localized TGF-beta 1 messenger RNA (mRNA) and protein to eosinophils. No other cell type contained TGF-beta 1 mRNA by in situ hybridization, whereas other leukocytes contained detectable TGF-beta 1 protein by immunohistochemistry. Eosinophils from four normal donors contained little or no detectable TGF-beta 1 protein by immunohistochemistry, whereas eosinophils from two of these four normal donors labeled weakly for TGF-beta 1 mRNA by in situ hybridization. These results show that eosinophils in the peripheral blood of patients with blood eosinophilia can express TGF-beta 1, but that eosinophils in the blood of normal donors contained little or no TGF-beta 1.
Wong
D T
DT
Department of Oral Medicine and Oral Pathology, Harvard School of Dental Medicine, Boston, MA 02115.
Elovic
A
A
Matossian
K
K
Nagura
N
N
McBride
J
J
Chou
M Y
MY
Gordon
J R
JR
Rand
T H
TH
Galli
S J
SJ
Weller
P F
PF
D000328:Adult; D015152:Blotting, Northern; D002469:Cell Separation; D004802:Eosinophilia / Q000097:blood* / Q000235:genetics; D004804:Eosinophils / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000502:physiology; D008297:Male; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000097:blood / Q000235:genetics; D012016:Reference Values; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D012313:RNA