Murine anti-human IL-6 monoclonal antibody prolongs the half-life in circulating blood and thus prolongs the bioactivity of human IL-6 in mice.
Human interleukin-6 (hIL-6) injected into mice increases antigen-specific antibody production. In the present study, we examined the possibility that anti-hIL-6 murine monoclonal antibody (MH-166) could neutralize this hIL-6 activity in vivo. Although MH166 completely neutralized hIL-6 activity in vitro, treatment in vivo with hIL-6 and MH166 combined unexpectedly increased both anti-dinitrophenyl (DNP) and anti-sheep red blood cell (SRBC) antibody production more than treatment with IL-6 alone did. To explain this phenomenon, the serum hIL-6 level was monitored following the MH166 administration. The hIL-6 level was significantly higher in the mice treated with both hIL-6 and MH166 than in the mice treated with hIL-6 alone during the 24 hr following hIL-6 administration. These results indicate that MH166 prolongs half-life of a xenogeneic hIL-6.
Mihara
M
M
Fuji-Gotemba Research Laboratories, Chugai Pharmaceutical Co. Ltd, Shizuoka, Japan.
Koishihara
Y
Y
Fukui
H
H
Yasukawa
K
K
Ohsugi
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000917:Antibody Formation / Q000187:drug effects*; D004140:Dinitrophenols / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006207:Half-Life; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D015850:Interleukin-6 / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains
D000911:Antibodies, Monoclonal; D004140:Dinitrophenols; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015850:Interleukin-6