The characterization of lectin-binding proteins of Chlamydia trachomatis as glycoproteins.
The 18 kDa and 32 kDa lectin binding proteins of Chlamydia trachomatis were characterized as glycoproteins by treatments with glycosidases. The proteins of the serovar L2 whole cell lysate were separated by SDS-PAGE and transferred to nitrocellulose paper. After treatment with an enzyme, the proteins were reacted with a biotinylated lectin. Each of the endoglycosidases tested affected the binding of the lectin to the protein. PNGase F inhibited the binding of Dolichos biflorus agglutinin (DBA), soybean agglutinin (SBA), and Ulex europaeus agglutinin I (UEAI) to both the 18 kDa and 32 kDa proteins. Endoglycosidase F and H inhibited the binding of these lectins to the 32 kDa protein completely and to the 18 kDa protein partially. In the exoglycosidase treatments, alpha-L-fucosidase prevented binding of only UEAI to the two proteins while beta-galactosidase inhibited the binding of SBA. Mannosidase abolished the binding of all the lectins tested. Neuraminidase had no effect. The proteins isolated by electroelution from the excised gels after SDS-PAGE were digested with an endoglycosidase. PNGase F-treated proteins showed a lower molecular weight mobility in which the lectin binding ability was destroyed. Endo-alpha-N-acetylgalactosaminidase had no effect. The polysaccharide stain of isolated proteins with p-phenylenediamine showed a positive reaction. Radiolabeling with [3H]glucosamine did not reveal the 18 kDa and 32 kDa proteins in autoradiography but [3H]galactose did.
Swanson
A F
AF
Department of Pathobiology, University of Washington, Seattle 98195.
Kuo
C C
CC
D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D001710:Biotin; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002692:Chlamydia trachomatis / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005688:Galactosamine / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008361:Mannosidases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D009439:Neuraminidase; D010655:Phenylenediamines; D013194:Staining and Labeling
D001426:Bacterial Proteins; D015815:Cell Adhesion Molecules; D037102:Lectins; D010655:Phenylenediamines; D001710:Biotin; D005688:Galactosamine; D008361:Mannosidases; D009439:Neuraminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; C029728:4-phenylenediamine; D005690:Galactose