title: The three-dimensional structure of frozen-hydrated Nudaurelia capensis beta virus, a T = 4 insect virus.
abstract: The three-dimensional structure of Nudaurelia capensis beta virus (N beta V) was reconstructed to 3.2-nm resolution from images of frozen-hydrated virions. The distinctly icosahedral capsid (approximately 40-nm diameter) contains 240 copies of a single 61-kDa protein subunit arranged with T = 4 lattice symmetry. The outer surface of unstained virions compares remarkably well with that previously observed in negatively stained specimens. Inspection of the density map, volume estimates, and model building experiments indicate that each subunit consists of two distinct domains. The large domain (approximately 40 kDa) has a cylindrical shape, approximately 4-nm diameter by approximately 4-nm high, and associates with two large domains of neighboring subunits to form a Y-shaped trimeric aggregate in the outer capsid surface. Four trimers make up each of the 20 planar faces of the capsid. Small domains (approximately 21 kDa) presumably associate at lower radii (approximately 13-16.5 nm) to form a contiguous, non-spherical shell. A T = 4 model, constructed from 80 trimers of the common beta-barrel core motif (approximately 20 kDa) found in many of the smaller T = 3 and pseudo T = 3 viruses, fits the dimensions and features seen in the N beta V reconstruction, suggesting that the contiguous shell of N beta V may be formed by intersubunit contacts between small domains having that motif. The small (approximately 1800 kDa), ssRNA genome is loosely packed inside the capsid with a low average density.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
authors.forename: N H
authors.initials: NH
authors.lastname: Olson
authors.forename: T S
authors.initials: TS
authors.lastname: Baker
authors.forename: J E
authors.initials: JE
authors.lastname: Johnson
authors.forename: D A
authors.initials: DA
authors.lastname: Hendry
mesh_terms: D000818:Animals; D002213:Capsid / Q000648:ultrastructure; D005615:Freezing; D007091:Image Processing, Computer-Assisted; D007304:Insect Viruses / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009036:Moths / Q000382:microbiology; D009942:Organometallic Compounds; D012328:RNA Viruses / Q000648:ultrastructure*; D012367:RNA, Viral / Q000648:ultrastructure; D013194:Staining and Labeling; D014764:Viral Proteins / Q000648:ultrastructure; D014867:Water
chemical_list: D009942:Organometallic Compounds; D012367:RNA, Viral; D014764:Viral Proteins; D014867:Water; C005460:uranyl acetate