Comparison of the effects of four hyperplastic agents on hamster cheek pouch mucosa.
Hyperplasia in the hamster cheek pouch was examined using 4 different hyperplastic agents: oil of turpentine 50% v/v in liquid paraffin; vitamin A palmitate 10% w/v in liquid paraffin; 12-O-tetradeconyl-phorbol-13-acetate 16nM in acetone; and ethylphenylpropiolate 0.04mM in acetone. Acetone, paraffin and untreated control groups were also examined. Cheek pouches were painted 3 times a week for up to 4 weeks with each solution. Samples were removed and prepared for light microscopy 24 hours after 2 weeks of painting and 24 hours, 6, 12 and 18 weeks after 4 weeks of painting. Hyperplasia was produced by application of turpentine, vitamin A and TPA after 2 weeks of application. Further increases in epithelial width occurred after 4 weeks of painting in the turpentine and vitamin A groups but a decrease was seen in the TPA group. Six weeks (vitamin A and TPA groups) or 12 weeks (turpentine group) after the completion of treatment the epithelium had a normal histological appearance. No differences between the control or EPP treated cheek pouch mucosa could be detected. Turpentine and vitamin A can be used as models of reversible hyperplasia in the hamster cheek pouch.
Shearer
B H
BH
Department of Oral Biology and Oral Pathology, School of Dentistry, University of Otago, Dunedin, New Zealand.
McMillan
M D
MD
Jenkinson
H F
HF
D000480:Alkynes / Q000494:pharmacology*; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D006224:Cricetinae; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004224:Diterpenes; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D006818:Hyalin; D006965:Hyperplasia; D007633:Keratins; D008297:Male; D009061:Mouth Mucosa / Q000187:drug effects* / Q000473:pathology*; D000084562:Retinyl Esters; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors; D014425:Turpentine / Q000494:pharmacology*; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000494:pharmacology
D000480:Alkynes; D004224:Diterpenes; D000084562:Retinyl Esters; C001500:keratohyalin; D014801:Vitamin A; C014794:retinol palmitate; C015202:ethylphenylpropiolate; D007633:Keratins; D013755:Tetradecanoylphorbol Acetate; D014425:Turpentine