Co-localization of enkephalin and TRH in perifornical neurons of the rat hypothalamus that project to the lateral septum.
Neurons of the lateral septal nucleus are surrounded by terminals immunoreactive for thyrotropin-releasing hormone (TRH) and enkephalin (Enk). Retrograde labeling from the lateral septum in combination with immunocytochemical analyses for Enk and TRH in colchicine-treated rats has revealed that nearly all Enk- (and TRH-) containing perifornical neurons project to the lateral septum. Immunostaining of adjacent, thin paraffin sections for either TRH or Enk and double staining of thick vibratome sections for the two peptides have shown that TRH and Enk immunoreactivities co-exist within the same neurons in the perifornical region of the hypothalamus.
Merchenthaler
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Functional Morphology Section, NIEHS, NIH, Research Triangle Park, NC 27709.
D000818:Animals; D001370:Axonal Transport; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology; D003078:Colchicine / Q000494:pharmacology; D004745:Enkephalins / Q000032:analysis*; D006735:Horseradish Peroxidase; D007031:Hypothalamus / Q000033:anatomy & histology* / Q000166:cytology; D007124:Immunoenzyme Techniques; D008297:Male; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis*; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins
D004745:Enkephalins; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D013973:Thyrotropin-Releasing Hormone; D006735:Horseradish Peroxidase; D003078:Colchicine