Identification of linear epitopes of the BPV-1 L1 protein recognized by sera of infected or immunized animals.
Sera from cattle that had been inoculated with BPV-1 virions or with recombinant L1 proteins and serum from a rabbit that had been immunized with SDS-denatured virions were evaluated for their reactivity with 466 overlapping synthetic peptides corresponding to 95% of the BPV-1 L1 protein. The late serological response of cattle to both intact virions and recombinant L1 proteins exhibited a similar profile of reactivity with approximately 70% (7 of 10) of L1 antigenic sites. However, the L1 serological response of the rabbit to SDS-denatured virions exhibited a significant difference from bovine serum antibodies in the profile of epitopes recognized, including a relative lack of response to major bovine epitopes located between L1 amino acids (AAs) 300-400. Importantly, only the sera from animals inoculated/immunized with intact virions was capable of neutralizing BPV-1 infectivity of murine C127 cells, suggesting that nonlinear epitopes are important for papillomavirus neutralization.
Jenson
A B
AB
Department of Pathology, Georgetown University, Washington, D.C.
Lim
P
P
Ghim
S
S
Cowsert
L
L
Olson
C
C
Lim
L Y
LY
Farquhar
C
C
Pilacinski
W
W
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D052688:Bovine papillomavirus 1 / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D007114:Immunization; D011817:Rabbits; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D014412:Tumor Virus Infections / Q000097:blood* / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D014771:Virion
D000596:Amino Acids; D000914:Antibodies, Viral; D000939:Epitopes; D011994:Recombinant Proteins; D014764:Viral Proteins