Immunohistochemical detection and correlation between MHC antigen and cell-mediated immune system in recurrent glioma by APAAP method.
As part of an on-going clinical trial of immunotherapy for recurrent malignant gliomas, using alkaline phosphatase-anti-alkaline phosphatase method with monoclonal antibodies, we investigated the correlation between expression of the major histocompatibility complex (MHC) and the subpopulation of tumor-infiltrating lymphocytes (TILs) in 38 glioma specimens (20 grade IV, 11 grade III, and 7 grade II) from 33 patients. Thirty specimens (78.9%) were positive to class I MHC antigen and 20 (52.6%) were positive to class II MHC antigen. The correlations between class I MHC antigen expression and the number of infiltrating T8 (p less than 0.01), and also between class II MHC antigen expression and the number of infiltrating T4 (p less than 0.05) were significant. We conclude that TILs are the result of immunoreaction (host-defense mechanism). 31.6% of specimens had perivascular infiltration of T cells. The main infiltrating lymphocyte subset in moderate to marked perivascular cuffing was T4. Our results may indicate that lack of MHC antigen on the glioma cell surface has a share in the poor immunogenicity in glioma-bearing patients. In addition, considering the effector/target ratio, the number of infiltrating lymphocytes against glioma cells was too small, so the immunological intervention seems to be essential in glioma therapy. Previous radiation therapy and chemotherapy, including steroid therapy, did not influence lymphocyte and macrophage infiltration.
Miyagi
K
K
Experimental and Clinical Laboratory, Huntington Medical Research Institutes, Pasadena, California.
Ingram
M
M
Techy
G B
GB
Jacques
D B
DB
Freshwater
D B
DB
Sheldon
H
H
D000328:Adult; D000368:Aged; D005910:Glioma / Q000276:immunology*; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D007111:Immunity, Cellular; D007150:Immunohistochemistry / Q000379:methods*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009423:Nervous System Neoplasms / Q000276:immunology*
D006680:HLA Antigens