[A comparative analysis of the biological activity of an acid-extractable brain protein and fibroblast growth factors].
An acid-soluble protein was isolated from bovine brain tissue using a combination of 5% HClO4 acidic extraction, cation-exchange chromatography and heparin-Sepharose affinity chromatography. This preparation had a biological activity similar to that of the fibroblast growth factor (FGF). It has stimulated neurite outgrowth in organotypic culture of chicken embryo dorsal root ganglia and also has stimulated a proliferation of human embryonic fibroblasts in the culture. The biological activity of isolated preparation was totally inhibited by anti-bFGF antibodies, while anti-aFGF antibodies had no effect. The bFGF showed a neurite stimulating activity in the organotypic culture that was completely abolished by the anti-bFGF, IgG, while the aFGF was inactive. This data, as well as cationic properties of isolated protein and its heparin-binding ability, enable us to postulate a high degree of homology of the brain acid-soluble protein and the bFGF. At the same time the relations of the isolated growth factor and other brain-derived heparin-binding growth factors are yet to be established.
Goncharova
V P
VP
Eropkin
M Iu
MIu
Chalisova
N I
NI
Akoev
G N
GN
Eropkina
E M
EM
Shevchenko
A A
AA
D000818:Animals; D000906:Antibodies / Q000187:drug effects; D002417:Cattle; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D002540:Cerebral Cortex / Q000502:physiology*; D002642:Chick Embryo; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D046508:Culture Techniques; D016220:Fibroblast Growth Factor 1 / Q000276:immunology / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000276:immunology / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects; D013268:Stimulation, Chemical
D000906:Antibodies; D009419:Nerve Tissue Proteins; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1
Sravnitel'nyĭ analiz biologicheskoĭ aktivnosti mozgovogo kislotoékstragiruemogo belka i faktory rosta fibroblastov.