A synthetic peptide elicits antibodies reactive with the external glycoprotein of human T cell lymphotropic virus type I.
A synthetic peptide derived from the external glycoprotein of human T cell lymphotropic virus type I (HTLV-I) (Env-5; amino acids 242 to 256) reacted with IgG antibodies in serum specimens from HTLV-I-infected individuals. C-terminal residues of Env-5 were crucial for the antibody reactivity. Polyclonal rabbit antibodies to Env-5 did not inhibit syncytium formation but such antibodies reacted specifically with gp68env and gp46env glycoproteins of HTLV-I in an immunoblot analysis. Immunoprecipitation of the surface-labelled MT-2 (HTLV-I-infected) cell line with anti-Env-5 precipitated the gp68env precursor protein. It was concluded that peptide Env-5 mimics a surface-exposed epitope on the HTLV-I external glycoprotein.
Lal
R B
RB
Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
Rudolph
D L
DL
Palker
T J
TJ
Coligan
J E
JE
Folks
T M
TM
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology*; D015481:HTLV-I Antibodies / Q000096:biosynthesis*; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008969:Molecular Sequence Data; D011233:Precipitin Tests
D000956:Antigens, Viral; C070924:Env-5 glycoprotein, HTLV-I; D000939:Epitopes; D006023:Glycoproteins; D015481:HTLV-I Antibodies; D007106:Immune Sera; D007074:Immunoglobulin G