Study of the activity of differentiation-induction in a Chinese herb-viscum alniformosanae. Part 1: In vitro induction of differentiation in HL-60 leukemic cell line by conditioned medium secreted from viscum alniformosanae-stimulated mononuclear cells.
A conditioned medium(CM), designated as 572-CMF-, was a Chinese herb viscum alniformosanae (V.A.) stimulated mononuclear cells. This CM has the capacity to induce the promyelocytic cell line HL-60 to differentiate into morphologically and functionally mature monocytoid cells. However, our results on the effect of a combination of 572 conditioned medium and IFN-r, TNF and IL-2 were neither synergistic nor additive. Further investigation of the nature of this conditioned medium remains to be performed.
Yang
L L
LL
Department of Medicine, Veterans General Hospital, Taipei, Taiwan.
Hsiao
K I
KI
Su
J L
JL
Liu
J
J
Chen
P M
PM
D002454:Cell Differentiation / Q000187:drug effects*; D002461:Cell Line, Transformed / Q000187:drug effects; D003470:Culture Media; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D007372:Interferons / Q000502:physiology; D007376:Interleukin-2 / Q000502:physiology; D007942:Leukemia, Experimental / Q000503:physiopathology*; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D014784:Mistletoe / Q000502:physiology*; D010587:Phagocytosis / Q000187:drug effects; D010946:Plants, Medicinal; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D003470:Culture Media; D004365:Drugs, Chinese Herbal; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D007372:Interferons