'Phytoantibodies': a general vector for the expression of immunoglobulin domains in transgenic plants.
Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immunohistochemical analysis. Immunoblotting showed that the polypeptide was stably expressed and accounted for up to 1% of the soluble protein fraction. It is therefore proposed that single immunoglobulin domains of suitable specificity expressed in plants may constitute an effective system to inhibit the activity of molecules involved in plant pathology or plant development.
Benvenuto
E
E
ENEA Dipartimento Ricera e Sviluppo Agroindustrali, Divisione Ingegneria Genetica C.P.2400, Roma, Italy.
Ordàs
R J
RJ
Tavazza
R
R
Ancora
G
G
Biocca
S
S
Cattaneo
A
A
Galeffi
P
P
D016960:Agrobacterium tumefaciens / Q000235:genetics; D015152:Blotting, Northern; D015153:Blotting, Western; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000502:physiology; D005822:Genetic Vectors / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008856:Microscopy, Fluorescence; D030821:Plants, Genetically Modified / Q000235:genetics; D010947:Plants, Toxic; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D013373:Substance P / Q000276:immunology; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D011993:Recombinant Fusion Proteins; D013373:Substance P