Comparison of an isoelectric focusing technique and high-performance liquid chromatography for determination of fetal hemoglobin levels.
The authors previously have reported measurements of fetal hemoglobin in infants in blood samples taken at autopsy using an isoelectric focusing (IEF) procedure. The current study was undertaken to compare this methodology with a high-performance liquid chromatography (HPLC) procedure. The correlation coefficient between the IEF and HPLC procedures was 0.938. The HPLC method is technically easier and has fewer disadvantages than the IEF procedure and is recommended for the determination of fetal hemoglobin levels.
Gilbert-Barness
E F
EF
Department of Pathology and Laboratory Medicine, Center for Health Sciences, University of Wisconsin, Madison.
Kenison
K S
KS
Shrago
E
E
Spennetta
T L
TL
Giulian
G G
GG
D002851:Chromatography, High Pressure Liquid; D005069:Evaluation Studies as Topic; D005319:Fetal Hemoglobin / Q000032:analysis*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007525:Isoelectric Focusing / Q000379:methods*; D012044:Regression Analysis
D005319:Fetal Hemoglobin