title: [Ultrastructural study of the antral mucosa to determine the presence of Helicobacter pylori and its association with chronic active gastritis].
abstract: We present the results of ultrastructural studies of gastric mucosa obtained through upper digestive endoscopy of eight patients with suspicious symptoms of non ulcer dyspepsia or peptic ulcer. Two were male and six female with a median age of 54 years. The urease test to determine the presence of HP and Hematoxylin-Eosin and Warthin-Starry staining techniques were practiced with the purpose of a better detection of bacteria and gastritis. We did not find any correlation between the endoscopic results and the presence of gastritis or HP. Of the 8 patients only one had negative results for HP and for Electron microscopy studies. Chronic active gastritis was seen with light microscopy in all of the cases. Three in this group presented mild focal dysplasia and one case intestinal metaplasia type IIa. The main ultrastructural findings were: a) diminished or absent microvilli underneath the bacteria; b) HP inside phagolysosomes in the cytoplasm of the epithelial cells; c) in some cases, the bacteria was attached to the cell membrane; d) the cellular wall of HP contains mucopolysaccharides and up to four polar flagella; e) there are polymorphonuclear leucocytes in the epithelium. We conclude that Electron Microscopy is not a routine method for studying HP, but it constitutes a good method to study pathogenicity of the bacteria.
authors.affiliation: Instituto de Anatomía Patológica, UCV, Unidad de Gastroenterología, Clínica El Avila, Caracas.
authors.forename: S
authors.initials: S
authors.lastname: Reyes Caldera
authors.forename: M E
authors.initials: ME
authors.lastname: Ruiz
authors.forename: M
authors.initials: M
authors.lastname: Matos Villalobos
authors.forename: E
authors.initials: E
authors.lastname: Guirola
authors.forename: J C
authors.initials: JC
authors.lastname: González
authors.forename: N
authors.initials: N
authors.lastname: Capozzolo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002462:Cell Membrane / Q000648:ultrastructure; D002908:Chronic Disease; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D005756:Gastritis / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D013194:Staining and Labeling; D014510:Urease
chemical_list: D014510:Urease
vernacular_title: Estudio ultraestructural de la mucosa antral para determinar Helicobacter pylori y su asociación con gastritis crónica activa.