[Application of silver acetate autometallography in histopathology: a new detection method for use in immunogold silver staining, lectin histochemistry and in situ hybridization].
Modern histochemical techniques allow the specific detection of tissue constituents in situ. Routinely formalin fixed, paraffin embedded tissues may present problems to the pathologist since destruction of substances can lead to false negative results. Immunogold-silver staining (IGSS) can be a way to overcome some of these problems. A highly efficient method allows silver enhancement to be carried out without the necessity of a dark box (silver acetate autometallography). To test the new modification, antisera to a variety of antigens were used on paraffin and semithin resin sections. The system was also tested for its applicability in multiple immunostaining techniques and for the in situ hybridisation of viral DNA and for demonstration of carbohydrates by lectin histochemistry. It showed a very high detection efficiency with low background staining.
Hacker
G W
GW
Institut für Pathologische Anatomie, Landeskrankenanstalten Salzburg, Osterreich.
Graf
A H
AH
Thurner
J
J
D000085:Acetates; D019342:Acetic Acid; D000230:Adenocarcinoma / Q000473:pathology; D003218:Condylomata Acuminata / Q000382:microbiology / Q000473:pathology; D004279:DNA, Viral / Q000032:analysis; D006046:Gold; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007202:Indicators and Reagents; D037102:Lectins; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D010336:Pathology / Q000379:methods*; D012834:Silver
D000085:Acetates; D004279:DNA, Viral; D007202:Indicators and Reagents; D037102:Lectins; D012834:Silver; D006046:Gold; D019342:Acetic Acid
Anwendung der Silberazetat-Autometallographie in der Histopathologie: Eine neue Darstellungsmethode zur Verwendung in Immunogold-Silver Staining, Lektin-Histochemie und in situ-Hybridisierung.