Gene expression for peptides in neurons of the petrosal and nodose ganglia in rat.
In situ hybridization was used to determine whether genes for neuropeptides [substance P/neurokinin A (SP/NKA), calcitonin gene-related peptide (CGRP), somatostatin (SOM), neuropeptide tyrosine (NPY) and cholecystokinin (CCK)] are expressed in inferior ganglia of the vagus (nodose) and glossopharyngeal (petrosal) nerves. Synthetic oligodeoxyribonucleotides, complementary to the cognate, mRNAs were labeled with [32P] or [35S], and hybridized to 10 microns thick sections of unperfused tissue which were then processed for film and emulsion autoradiography. We found numerous, clustered neuronal perikarya throughout the nodose and petrosal ganglia that expressed preprotachykinin A (SP/NKA) and CGRP mRNAs to varying degrees. Neurons expressing preproSOM mRNA were less abundant and more scattered throughout both ganglia. Notably, we found mRNA for NPY in cells (usually 5-10 per section) in both ganglia. To our knowledge, this is first evidence for NPY in these sensory ganglia. In contrast to previous immunohistochemical findings, we found no evidence for expression of preproCCK in either the nodose or petrosal ganglia. The present findings demonstrate that cells of the nodose and petrosal ganglia express the genes for a number of neuropeptides that are presumably involved with transmission of visceral sensory afferent information to higher order neurons of the central nervous system.
Czyzyk-Krzeska
M F
MF
Department of Physiology, University of North Carolina, Chapel Hill 27599.
Bayliss
D A
DA
Seroogy
K B
KB
Millhorn
D E
DE
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis / Q000235:genetics; D002766:Cholecystokinin / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D005930:Glossopharyngeal Nerve / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D015288:Neurokinin A / Q000096:biosynthesis / Q000235:genetics; D009478:Neuropeptide Y / Q000096:biosynthesis / Q000235:genetics; D009479:Neuropeptides / Q000235:genetics*; D009620:Nodose Ganglion / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D011498:Protein Precursors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000096:biosynthesis / Q000235:genetics; D013373:Substance P / Q000096:biosynthesis / Q000235:genetics; D015320:Tachykinins / Q000494:pharmacology
D009478:Neuropeptide Y; D009479:Neuropeptides; D015345:Oligonucleotide Probes; D011498:Protein Precursors; D012333:RNA, Messenger; D015320:Tachykinins; C044206:preprotachykinin; D013373:Substance P; D013004:Somatostatin; D015288:Neurokinin A; D002766:Cholecystokinin; D015740:Calcitonin Gene-Related Peptide