title: Atrial natriuretic factor increases cyclic GMP and cyclic AMP levels in a directly photosensitive pineal organ.
abstract: Atrial natriuretic factor (ANF) stimulates accumulation of cyclic GMP in a photosensitive organ, as evidenced for the first time in cultured trout pineals. Stimulation was rapid (within a few min), dose-dependent, and stronger in organs cultured in darkness than in those cultured under light. After 30 min in the dark, (i) cyclic AMP levels were slightly increased at 10(-7) mole/l of ANF, (ii) cyclic GMP and cyclic AMP increased dramatically after inhibition of the phosphodiesterases by isobutylmethylxanthine (IBMX), (iii) ANF and IBMX effects were more than additive on cyclic GMP, (iv) pertussis toxin decreased the cyclic GMP response to ANF. These responses were affected by light. The possibility that cyclic GMP might be a second messenger of both light and chemical (ANF) inputs, in pineal photoreceptor cells, is hypothetized.
authors.affiliation: Laboratoire de Biologie Cellulaire, URA CNRS 290, Université de Poitiers, France.
authors.forename: J
authors.initials: J
authors.lastname: Folcòn
authors.forename: C
authors.initials: C
authors.lastname: Thibault
authors.forename: J L
authors.initials: JL
authors.lastname: Blazquez
authors.forename: H
authors.initials: H
authors.lastname: Vaudry
authors.forename: N
authors.initials: N
authors.lastname: Ling
authors.forename: J P
authors.initials: JP
authors.lastname: Colin
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D003624:Darkness; D008027:Light; D009599:Nitroprusside / Q000494:pharmacology; D009924:Organ Culture Techniques; D037342:Pertussis Toxin; D010870:Pineal Gland / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D014337:Trout; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D010566:Virulence Factors, Bordetella; D009599:Nitroprusside; D009320:Atrial Natriuretic Factor; D000242:Cyclic AMP; D037342:Pertussis Toxin; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine