title: Ion channel activities of cultured rat mesangial cells.
abstract: We used the patch-clamp technique to clarify the nature of ion channels in renal mesangial cells in culture. In the cell-attached mode most patches were silent in the absence of agonists. In some patches a 25-pS nonselective channel was observed. This 25-pS cation channel was consistently observed in inside-out patches, and it was activated by intracellular Ca2+. Excised patch experiments also revealed the existence of a 40-pS K+ channel, which was activated by intracellular Ca2+. This 40-pS K+ channel was observed infrequently in the cell-attached mode. The activities of both channels were increased by arginine vasopressin or angiotensin II, resulting from an increase in intracellular Ca2+ concentration.
authors.affiliation: Fourth Department of Internal Medicine, University of Tokyo School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Matsunaga
authors.forename: N
authors.initials: N
authors.lastname: Yamashita
authors.forename: Y
authors.initials: Y
authors.lastname: Miyajima
authors.forename: T
authors.initials: T
authors.lastname: Okuda
authors.forename: H
authors.initials: H
authors.lastname: Chang
authors.forename: E
authors.initials: E
authors.lastname: Ogata
authors.forename: K
authors.initials: K
authors.lastname: Kurokawa
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D004553:Electric Conductivity / Q000187:drug effects; D005071:Evoked Potentials / Q000187:drug effects; D005920:Glomerular Mesangium / Q000187:drug effects / Q000502:physiology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D015221:Potassium Channels / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007473:Ion Channels; D015221:Potassium Channels; D000804:Angiotensin II; D001127:Arginine Vasopressin