A nontoxic, idiotope vaccine against gram-negative bacterial infections.
Experiments were performed to test the ability of mouse antiidiotopic mAb, specific for an antilipid A mAb, to act as a vaccine against gram-negative bacterial infections. Lipid A is a conserved region of bacterial LPS. Immunization with the antiidiotopic antibodies, coupled to an immunogenic carrier protein (hemocyanin), specifically induced anti-LPS antibody responses in animals from different species. In a mouse model, this immunization resulted in protection against both lethal gram-negative bacteremia and endotoxemia. The antiidiotopic antibodies, however, did not stimulate endotoxin-associated bioactivities, such as induction of TNF and IL-1. These results support the hypothesis that an idiotope vaccine can stimulate beneficial protective immunity against gram-negative infections without the toxicity inherent in LPS.
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Department of Molecular Immunology, Roswell Park Cancer Institute, Buffalo, NY 14263.
Ward
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Apicella
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Ward
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D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000627:therapeutic use*; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D016470:Bacteremia / Q000517:prevention & control; D001424:Bacterial Infections / Q000517:prevention & control*; D001428:Bacterial Vaccines / Q000276:immunology*; D004731:Endotoxins / Q000097:blood / Q000276:immunology; D005260:Female; D006090:Gram-Negative Bacteria / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007375:Interleukin-1 / Q000097:blood; D008050:Lipid A / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D004731:Endotoxins; D007130:Immunoglobulin Idiotypes; D007375:Interleukin-1; D008050:Lipid A; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha