Effect of an immunostimulatory substance of Klebsiella pneumoniae on inflammatory responses of human granulocytes, basophils and platelets.
RU 41740 (Biostim), a biological response modifier of bacterial origin obtained from the 01.K2 strain of K. pneumoniae, was studied with regard to its effect to modulate the chemiluminescence response, phagocytosis and leukotriene formation from polymorphonuclear leukocytes (PMNs), the histamine release from basophils and the aggregation of human platelets. For histamine release human basophils were analyzed with either the calcium ionophore A23187 or hemolysin producing bacteria. RU 41740 reduced the E. coli K12 pANN5211 induced histamine release whereas the calcium ionophore A23187 induced histamine release was not affected. The studies with regard to adherence and phagocytosis showed a significant increase in phagocytosis for S. aureus in the presence of RU 41740. With regard to the chemiluminescence response of human granulocytes, donor cells were divided into cells with a high and a low response. The chemiluminescence response of high responder cells towards a potent stimulus, e.g. the calcium ionophore A23187, was decreased by RU 41740 whereas weak stimuli (e.g. E. coli K12) were not affected. In contrast to these results the chemiluminescence response of low responder cells to a weak stimulus e.g. E. coli K12 was increased by RU 41740 and the potent stimuli were not affected. RU 41740 also modulates the eicosanoid synthesis of human polymorphonuclear granulocytes. For the calcium ionophore A23187 induced leukotriene production an increase of LTB4 and a decrease of LTC4 by RU 41740 was observed. Furthermore, preincubation of platelets with various concentrations of RU 41740 for 5 min shows an inhibition of platelet aggregation, when an additional stimulation with the calcium ionophore A23187 was carried out.
Scheffer
J
J
Lehrstuhl für Medizinische Mikrobiologie und Immunologie, Ruhr-Universität, Bochum, Fed. Rep. of Germany.
Ounis
I
I
König
W
W
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000032:analysis / Q000494:pharmacology; D001491:Basophils / Q000187:drug effects*; D001792:Blood Platelets / Q000187:drug effects*; D000001:Calcimycin / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006098:Granulocytes / Q000187:drug effects*; D006460:Hemolysin Proteins; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007104:Immune Adherence Reaction; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology*; D007711:Klebsiella pneumoniae / Q000276:immunology*; D015289:Leukotrienes / Q000096:biosynthesis; D008163:Luminescent Measurements; D010587:Phagocytosis / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D015289:Leukotrienes; D010975:Platelet Aggregation Inhibitors; D000001:Calcimycin; C037567:glycoprotein, Klebsiella pneumoniae