Structure and localization of the human insulin-like growth factor-binding protein 2 gene.
Insulin-like growth factor binding proteins (IGFBPs) are extracellular proteins that specifically bind IGF and modulate their effects. The human IGFBP2 gene was studied and shown to be localized to chromosome 2 region q33-q34, by somatic cell hybrid analysis and in situ hybridization. Structural characterization of the gene showed that it consists of four exons with three introns of lengths 27.0, 1.0, and 1.9 kilobase-pairs. Comparison of the encoded protein sequence of each exon in IGFBP1, 2, and 3 reveals the highest amino acid identity, 28%, in exon 1, while the lowest was found in exon 2. However, pairwise sequence comparisons demonstrate 50% identity between the protein sequences encoded by exon 4 in IGFBP1 and 2, while their respective identities with IGFBP3 are only 25 and 30%.
Ehrenborg
E
E
Department of Clinical Genetics, Karolinska Institute, Stockholm, Sweden.
Vilhelmsdotter
S
S
Bajalica
S
S
Larsson
C
C
Stern
I
I
Koch
J
J
Brøndum-Nielsen
K
K
Luthman
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D003360:Cosmids; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D005260:Female; D005796:Genes; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology; D018971:Insulin-Like Growth Factor Binding Protein 2; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010920:Placenta / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D002352:Carrier Proteins; D018971:Insulin-Like Growth Factor Binding Protein 2; D015345:Oligonucleotide Probes; D004247:DNA