Production of inositol phosphates and reactive oxygen metabolites in quartz-dust-stimulated human polymorphonuclear leukocytes.
The present paper explores phosphoinositide turnover in quartz-stimulated human polymorphonuclear leukocytes. Separation of inositol phosphates was carried out with a new ion-pair, reverse-phase high performance liquid chromatographic method applying a gentle tetrabutyl ammonium phosphate buffer gradient. The method separates inositol monophosphates, inositol 1,4-bisphosphate, inositol trisphosphates and inositol 1,3,4,5-tetrakisphosphate. Reactive oxygen metabolites, indices for leukocyte activation, were measured with a luminometric assay. Quartz increased the production of reactive oxygen metabolites, preceded by facilitated inositol phosphate turnover. This finding provides evidence that inositol phosphate second messengers may be involved in quartz-induced leukocyte activation and subsequent production of reactive oxygen metabolites.
Tuomala
M H
MH
National Public Health Institute, Department of Environmental Hygiene and Toxicology, Kuopio, Finland.
Hirvonen
M R
MR
Savolainen
K M
KM
D002851:Chromatography, High Pressure Liquid; D004391:Dust; D005609:Free Radicals; D006801:Humans; D007295:Inositol Phosphates / Q000096:biosynthesis*; D049449:Luminescence; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D011791:Quartz / Q000276:immunology*
D004391:Dust; D005609:Free Radicals; D007295:Inositol Phosphates; D011791:Quartz; D010100:Oxygen