Molecular cloning and expression of the 01 rfb region from a pyelonephritic Escherichia coli 01:H1:K7.
The genes responsible for the biosynthesis of the O1 polysaccharide from a human pyelonephritic Escherichia coli were cloned and expressed in a rfb-deleted E. coli K-12 strain. Deletion analysis of the clone demonstrated that a DNA fragment size larger than 6.7 kb and smaller than 10 kb is responsible for O1-antigen biosynthesis.
Ding
M J
MJ
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030.
Svanborg
C
C
Haraguchi
G E
GE
Hull
R A
RA
Hull
S I
SI
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D015870:Gene Expression; D006801:Humans; D007231:Infant, Newborn; D007674:Kidney Diseases / Q000382:microbiology; D008070:Lipopolysaccharides / Q000032:analysis; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000235:genetics*
D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial