Non-specific oligodendrocyte cytotoxicity mediated by soluble products of activated T cell lines.
Supernates from myelin basic protein (MBP)-reactive T cell lines have been tested by a battery of assays for cytotoxicity against oligodendrocytes in vitro. All supernates tested demonstrated cytotoxic activity in both a dose- and time-dependent manner that ranged from 29.1% to 55.8% after 48 h incubation. Oligodendrocyte cytotoxicity mediated by MBP-reactive T cell lines was not antigen specific since lines reactive with purified protein derivative (PPD) of tuberculin showed similar cytotoxicity. Supernates cytotoxic to oligodendrocytes were equally effective against syngeneic and non-syngeneic target cells, but had no effect upon astrocytes. Neutralization studies revealed that oligodendrocyte cytotoxicity mediated by MBP-reactive T cell supernates could only be partially attributed to lymphotoxin/tumor necrosis factor activity, and was not associated with perforin, serine proteinase or N-methyl-D-aspartate (NMDA) receptor agonists.
Selmaj
K
K
Department of Pathology (Neuropathology), Albert Einstein College of Medicine, Bronx.
Cross
A H
AH
Farooq
M
M
Brosnan
C F
CF
Raine
C S
CS
D000818:Animals; D001253:Astrocytes / Q000276:immunology; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005260:Female; D008213:Lymphocyte Activation; D051379:Mice; D004676:Myelin Basic Protein / Q000276:immunology; D009500:Neutralization Tests; D009836:Oligodendroglia / Q000166:cytology / Q000276:immunology*; D012995:Solubility; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D004676:Myelin Basic Protein