Biosynthesis of vitamin B12 in Pseudomonas denitrificans: the biosynthetic sequence from precorrin-6y to precorrin-8x is catalyzed by the cobL gene product.
A protein catalyzing methylation at C-5 and C-15 and decarboxylation of the acetic acid side chain at C-12 on precorrin-6y to yield precorrin-8x was purified to homogeneity from a recombinant strain of Pseudomonas denitrificans. It was sequenced at the N terminus and shown to be encoded by the cobL gene.
Blanche
F
F
Département Analyse, Centre de Recherche de Vitry-Alfortville, Vitry-sur-Seine, France.
Famechon
A
A
Thibaut
D
D
Debussche
L
L
Cameron
B
B
Crouzet
J
J
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D003653:Decarboxylation; D005798:Genes, Bacterial; D008745:Methylation; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000201:enzymology*; D014578:Uroporphyrins / Q000378:metabolism*; D014805:Vitamin B 12 / Q000096:biosynthesis*
D001426:Bacterial Proteins; D014578:Uroporphyrins; C072473:precorrin 6y; C072475:precorrin 8x; D014805:Vitamin B 12