Demonstration of a transcription element in vitro between the capping site and translation initiation site of the mouse myelin basic protein gene.
A transcription element was identified, by in vitro analyses, just downstream from the capping site of the mouse myelin basic protein (MBP) gene. Deletion of this element caused a dramatic drop of transcription efficiency in mouse brain, rat liver and HeLa cell nuclear extracts, regardless of the form of DNA being closed circular or linear form. DNase I footprint analysis demonstrated the presence of a ubiquitous trans-acting factor for this region. This element functioned even when it is located in the normal direction downstream from the adenovirus major late promoter. Mutation analysis suggested that an essential part of the downstream element was located between +25 and +45.
Tamura
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T
Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, Japan.
Mikoshiba
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K
D000818:Animals; D001483:Base Sequence; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D006367:HeLa Cells / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D004676:Myelin Basic Protein / Q000235:genetics*; D014176:Protein Biosynthesis; D051381:Rats; D011940:Receptor Aggregation / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D016385:TATA Box; D015534:Trans-Activators / Q000235:genetics; D014158:Transcription, Genetic
D004676:Myelin Basic Protein; D015534:Trans-Activators; D003850:Deoxyribonuclease I