Sequence of EndoA gene encoding mouse cytokeratin and its methylation state in the CpG-rich region.
A genomic clone obtained from mouse liver DNA using a mouse cytokeratin EndoA cDNA probe revealed the complete sequence of the EndoA gene. The gene is divided into nine exons and the exon-intron pattern has been conserved compared to that of other type-II cytokeratin-encoding genes. The 5' upstream, 3' downstream and first and third introns contain potential regulatory sequences, including polyoma virus enhancer motifs (PEA1 and PEA3) and AP-1 elements. The 5' regions upstream of the EndoA, EndoB and Ck8 genes contain homologous sequences surrounding the TATA boxes. In addition, a CpG dinucleotide cluster region was located around the first exon. This CpG cluster region was found to be hypomethylated in endodermal PYS-2 cells, retinoic acid-treated F9 cells, and F9 embryonal carcinoma cells, but hypermethylated in BALB/C 3T3 fibroblast cells that do not express EndoA. These findings may provide a clue to understanding the molecular mechanisms of EndoA gene expression.
Tamai
Y
Y
Department of Microbial Genetics, Osaka University, Japan.
Takemoto
Y
Y
Matsumoto
M
M
Morita
T
T
Matsushiro
A
A
Nozaki
M
M
D000818:Animals; D001483:Base Sequence; D004247:DNA; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D006801:Humans; D007438:Introns; D007633:Keratins / Q000235:genetics* / Q000378:metabolism; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D015226:Dinucleoside Phosphates; D007633:Keratins; C015772:CpG dinucleotide