Xenopus c-raf proto-oncogene: cloning and expression during oogenesis and early development.
We have isolated and characterized a cDNA which contains the entire coding sequence of Xenopus laevis raf protein. raf mRNA is identified as a member of the class of maternal RNAs. It is already relatively abundant at the beginning of oogenesis and is stable at least until the midblastula transition. The RNA is also detected later during embryogenesis in particular in gastrula, neurula, tailbud and feeding tadpole. We have also found the RNA in several adult tissues (skin, testis, stomach, intestine) at different levels.
Le Guellec
R
R
CNRS URA 256, Université de Rennes I, France.
Couturier
A
A
Le Guellec
K
K
Paris
J
J
Le Fur
N
N
Philippe
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics* / Q000254:growth & development
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012313:RNA; D004247:DNA; D011494:Protein Kinases; D019908:Proto-Oncogene Proteins c-raf