title: Transcriptionally active regions in the genome of the archaebacterium Haloferax volcanii.
abstract: Transcriptionally active regions of the Haloferax volcanii genome were mapped by hybridization of radiolabeled cDNA to Southern blots of our minimal set of overlapping cosmid clones covering 96% of the 4.1-Mbp genome. Transcription during exponential growth occurred in nearly every region of the 2,920-kbp chromosome. Large parts of the 690- and the 86-kbp plasmids were transcribed, but the 440-kbp plasmid showed little expression. Transcription after a 40-min heat shock at 65 degrees C was generally reduced, apart from a small set of strongly expressed loci all situated on the chromosome.
authors.affiliation: Department of Biology, University of Ottawa, Ontario, Canada.
authors.forename: B A
authors.initials: BA
authors.lastname: Trieselmann
authors.forename: R L
authors.initials: RL
authors.lastname: Charlebois
mesh_terms: D001105:Archaea / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins; D015139:Blotting, Southern; D002455:Cell Division; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016680:Genome, Bacterial; D006360:Heat-Shock Proteins / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; C035041:endodeoxyribonuclease MluI; D015252:Deoxyribonucleases, Type II Site-Specific