In vivo persistence of a HIV-1-encoded HLA-B27-restricted cytotoxic T lymphocyte epitope despite specific in vitro reactivity.
A large number of human immunodeficiency virus type 1 (HIV-1) specific HLA-restricted cytotoxic T cell (CTL) epitopes have been mapped, including an HLA-B27-restricted immunodominant epitope within p25gag. Accordingly, this segment of the HIV-1 provirus was amplified by the polymerase chain reaction from DNA derived from fresh uncultured peripheral blood mononuclear cells (PBMC) of four HLA-B27 HIV-1-infected individuals. In all cases the majority of infected PBMC bore sequences encoding the HLA-B27-restricted peptide. CTL escape mutants had not accumulated in vivo 8 and 14 months later despite demonstrable CTL activity in vitro. These data emphasize the importance of silently infected lymphocytes in evading immune surveillance.
Meyerhans
A
A
Laboratoire de Rétrovirologie Moléculaire, Institut Pasteur, Paris, France.
Dadaglio
G
G
Vartanian
J P
JP
Langlade-Demoyen
P
P
Frank
R
R
Asjö
B
B
Plata
F
F
Wain-Hobson
S
S
D000595:Amino Acid Sequence; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D015683:Gene Products, gag / Q000737:chemistry / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D015796:HLA-B27 Antigen / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000939:Epitopes; D015683:Gene Products, gag; D015488:HIV Antigens; D015796:HLA-B27 Antigen