Structure of the L2 lipopolysaccharide core oligosaccharides of Neisseria meningitidis.
Three different oligosaccharides were identified following mild acid hydrolysis of the lipopolysaccharide obtained from Neisseria meningitidis serotype 2 and their structures elucidated by combined chemical and physical techniques. The use of 500 MHz 1H nmr in both one- and two-dimensional modes as well as nuclear Overhauser effect experiments were employed. To assist in the structural assignments the oligosaccharides were also degraded by chemical and enzymatic procedures to smaller fragments. The oligosaccharides were all triantennary nonasaccharides in which the longest antenna terminates in lacto-N-neotetraose. Two of the nonasaccharides (major components), not separable by column chromatography, were distinguishable only by their different patterns of phosphorylethanolamine substitution and the third minor component by the absence of this substituent.
Gamian
A
A
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada.
Beurret
M
M
Michon
F
F
Brisson
J R
JR
Jennings
H J
HJ
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002853:Chromatography, Liquid; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000737:chemistry*
D008070:Lipopolysaccharides