Ontogeny of membrane and soluble amino-oligopeptidases in rat intestine.
Intestinal amino-oligopeptidase (AOP) plays an essential role in protein digestion. To characterize its postnatal development, we measured AOP activity in intestinal membrane and cytosolic fractions in suckling and weaned rats, compared the subunit structures of the membrane and soluble enzymes, and assessed the biochemical relationship of these peptidases. At weaning, jejunal membrane AOP activity doubled while soluble AOP activity in the ileum fell abruptly. The maturational increase in the molecular mass of ileal membrane AOP was due to alterations in the N-linked glycosylation of this protein. Ileal membrane and soluble AOP exhibited similar substrate affinities, pH optima, inhibition characteristics, and antigenic epitopes. However, soluble AOP was 25-35 kDa smaller than the membrane enzyme. Peak incorporation of [35S]methionine into ileal brush-border AOP preceded maximal radioactivity in soluble AOP, suggesting that the membrane peptidase is a precursor of the soluble enzyme. We conclude that membrane and soluble AOP are closely related proteins with distinct developmental profiles and that the soluble peptidase may be derived from endocytosis of the membrane enzyme.
Reisenauer
A M
AM
Department of Pediatrics, Stanford University, California 94305.
Lee
E A
EA
Castillo
R O
RO
D000626:Aminopeptidases / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D018826:CD13 Antigens; D003429:Cross Reactions; D007422:Intestines / Q000201:enzymology* / Q000254:growth & development; D037102:Lectins; D008566:Membranes / Q000201:enzymology / Q000254:growth & development; D008715:Methionine / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility
D037102:Lectins; D008715:Methionine; D000626:Aminopeptidases; D018826:CD13 Antigens