title: Enzyme immunoassay of a substance P-like immunoreactive substance in human plasma and saliva.
abstract: A sensitive and specific double-antibody enzyme immunoassay (EIA) for a substance P (SP)-like immunoreactive substance (SP-IS) was developed. For competitive reactions, the SP-antibody was incubated with SP standard (or sample) and beta-D-galactosidase labeled Tyr8-SP (delayed addition). Free and antibody-bound enzyme hapten were separated by using an anti-rabbit immunoglobulin G coated immunoplate. Activity of the enzyme on the plate was fluorometrically determined. The present immunoassay allows detection of 0.4 to 10 fmol/well of SP. Using the present EIA, SP-ISs in human saliva and plasma were determined. The level of SP-IS in human saliva was about 7 pmol/l, which was almost three times higher than that in human plasma.
authors.affiliation: Department of Hospital Pharmacy, Medical College of Oita, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Takeyama
authors.forename: K
authors.initials: K
authors.lastname: Mori
authors.forename: F
authors.initials: F
authors.lastname: Takayama
authors.forename: K
authors.initials: K
authors.lastname: Kondo
authors.forename: K
authors.initials: K
authors.lastname: Kitagawa
authors.forename: N
authors.initials: N
authors.lastname: Fujii
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007124:Immunoenzyme Techniques; D012463:Saliva / Q000737:chemistry*; D013373:Substance P / Q000032:analysis* / Q000097:blood / Q000276:immunology
chemical_list: D013373:Substance P