[Effect of various blood components and additive solutions on phytohemagglutinin (PHA)-induced lymphocyte proliferation].
The effect of red blood cells (RBC), fresh plasma (FP), fresh frozen plasma (FFP) and different storage solutions (e.g. CPDA-1, CPD, PAGGS-M, SAG-M, Adsol) on the immune response was investigated in human lymphocyte cultures. The inhibitory activity was measured by determining the effect of different components on the PHA-induced T-cell proliferation. Our results indicate that the inhibitory effect is caused by an as yet unidentified plasma factor (e.g. anti-idiotypic antibodies, soluble HLA-class I or II antigens). In contrast, pure RBC or blood storage solutions revealed only minimal effects on the T-cell response.
Sibrowski
W
W
Univ. Klinik Hamburg, Abt. für Transfusionsmedizin.
Danker
S
S
Wegner
W
W
Kühl
P
P
D016913:Blood Component Transfusion; D001793:Blood Preservation; D004906:Erythrocyte Count; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D010835:Phytohemagglutinins / Q000276:immunology*; D010949:Plasma / Q000276:immunology*
D010835:Phytohemagglutinins
Einfluss verschiedener Blutkomponenten und Additiv-Lösungen auf die Phytohämagglutinin (PHA)-induzierte Lymphozyten-Proliferation.