Immune recognition of linear epitopes in peptide fragments of epithelial mucins.
Anti-human milk fat globule membrane monoclonal antibodies HMFG-1 and HMFG-2 recognize epitopes within the protein core of human polymorphic epithelial mucin (PEM). These have been identified as PDTR and DTR, respectively. Using the solid phase synthesis of immobilized tetrameric peptides, we have systematically investigated the contribution of each amino acid in the immune recognition of the PDTR domain by the antibodies HMFG-1 and HMFG-2. The findings obtained have been interpreted with respect to the presence of, and requirements for elements of secondary structure, which have been identified in this region of the PEM protein core.
Briggs
S
S
Cancer Research Campaign Laboratories, University of Nottingham, U.K.
Price
M R
MR
Tendler
S J
SJ
D000911:Antibodies, Monoclonal; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology; D018396:Mucin-1; D009077:Mucins / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008562:Membrane Glycoproteins; D018396:Mucin-1; D009077:Mucins; D010446:Peptide Fragments