title: Monoclonal antibodies to secreted antigens of Brugia malayi define a cross-reactive non-phosphocholine determinant on helminth parasites.
abstract: The excretory-secretory (ES) antigens of the filarial parasite Brugia malayi adult (BmA) and microfilariae (MF) were analysed for differences in their protein composition by two-dimensional gel electrophoresis. Both BmA and MF biosynthetically labelled with [3H]-leucine released a 200 kD molecule with pI (isoelectric point) ranging from 5.1 to 6.8. A monoclonal antibody (MoAb) to phosphocholine (PC) immunoprecipitated the 200 kD molecule with a PI of 5.1-5.3 from both BmA and MF. Immunization of CBA/N mice with ES antigens resulted in MoAb that reacted with PC, as well as some that did not (ES-1, ES-2 and ES-3). The epitopes recognized by these non-PC MoAb varied greatly in their molecular weight, heat-resistance, TCA-solubility and metaperiodate-sensitivity, which suggests a proteoglycan nature of the epitope. An antigen capture assay using ES-1 and ES-3 detected a circulating filarial antigen not only in patients infected with Wuchereria bancrofti (77-83% of those with asymptomatic microfilaraemia and 52-60% of those with chronic lymphatic obstruction) but also in serum from patients infected with other helminth parasites (12-100%). Indeed, the epitope recognized by these MoAb was also present on other helminth parasites, which suggests conservation of a carbohydrate-like prosthetic group(s) on diverse helminth species.
authors.affiliation: Department of Preventive Medicine, Uniformed Services University of Health Sciences, Bethesda, Maryland 20894.
authors.forename: R B
authors.initials: RB
authors.lastname: Lal
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D002009:Brugia / Q000276:immunology*; D003429:Cross Reactions; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D015801:Helminth Proteins; D008842:Microfilariae / Q000276:immunology*; D014119:Toxocara / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000939:Epitopes; D015801:Helminth Proteins; C045159:TES-26 protein, Toxocara canis