Sequences involved in brain-specific in vitro transcription from the core promoter of the mouse myelin basic protein gene.
In the previous study, we have shown that a short DNA stretch from -35 to -17 in the core promoter of the mouse myelin basic protein (MBP) gene is mainly responsible for brain-specificity in in vitro transcription. In this study, we found from intensive mutation analysis that the TATA-box sequence at -34 is not critical, but sequences downstream from the TATA-box especially around -20 were important for brain-specificity. The existence of a tissue-specific factor for the MBP core promoter different from the TATA-box-binding factor TFIID is implicated.
Tamura
T
T
Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, Japan.
Sumita
K
K
Mikoshiba
K
K
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D004252:DNA Mutational Analysis; D008099:Liver / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics* / Q000502:physiology; D051381:Rats; D016385:TATA Box / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
D004676:Myelin Basic Protein; D014157:Transcription Factors