Immunolocalization of types V and XI collagen in cartilage using monoclonal antibodies.
Monoclonal antibodies produced against pepsin-solubilized newborn rat skin type V collagen [alpha 1(V)]2 alpha 2(V), and chondrosarcoma type XI collagen [alpha 1(XI) alpha 2(XI) alpha 3(XI)] are used to localize the collagens in sections of the chondrosarcoma as well as the normal rat knee joint by indirect immunofluorescence. Immunostaining for type V collagen shows strong cellular staining of chondrocytes; while the interstitial matrix as well as the lacunae are not stained. In contrast, antitype XI stains not only chondrocytes, but the extracellular compartments as well. In ELISA, rat anti-type XI collagen reacts with its native antigen, but does not cross-react with native types I, II, III, or V collagen from rat. The distinct locations of type V and XI collagens in cartilaginous tissue suggest varied functional roles for these constituents in the tissue.
Furuto
D K
DK
Research Center in Oral Biology, University of Alabama, Birmingham 35294.
Gay
R E
RE
Stewart
T E
TE
Miller
E J
EJ
Gay
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D002356:Cartilage / Q000737:chemistry*; D002813:Chondrosarcoma / Q000737:chemistry; D003094:Collagen / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D003094:Collagen