Cloning of the cocaine-sensitive bovine dopamine transporter.
A cDNA encoding the dopamine transporter from bovine brain substantia nigra was identified on the basis of its structural homology to other, recently cloned, neurotransmitter transporters. The sequence of the 693-amino acid protein is quite similar to those of the rat gamma-aminobutyric acid, human norepinephrine, and rat serotonin transporters. Dopamine transporter mRNA was detected by in situ hybridization in the substantia nigra but not in the locus coeruleus, raphe, caudate, or other brain areas. [3H]Dopamine accumulation in tissue culture cells transfected with the cDNA was inhibited by amphetamine, cocaine, and specific inhibitors of dopamine transport, including GBR12909.
Usdin
T B
TB
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892.
Mezey
E
E
Chen
C
C
Brownstein
M J
MJ
Hoffman
B J
BJ
D000595:Amino Acid Sequence; D000818:Animals; D016375:Antisense Elements (Genetics); D001483:Base Sequence; D002352:Carrier Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D003042:Cocaine / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism*; D018492:Dopamine Antagonists; D050483:Dopamine Plasma Membrane Transport Proteins; D015723:Gene Library; D007700:Kinetics; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D009693:Nucleic Acid Hybridization; D010879:Piperazines / Q000494:pharmacology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015347:RNA Probes; D012689:Sequence Homology, Nucleic Acid; D013378:Substantia Nigra / Q000187:drug effects / Q000502:physiology*; D014162:Transfection; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics
D016375:Antisense Elements (Genetics); D002352:Carrier Proteins; D018492:Dopamine Antagonists; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D010879:Piperazines; D015347:RNA Probes; D012313:RNA; C043425:vanoxerine; D014446:Tyrosine 3-Monooxygenase; D003042:Cocaine; D004298:Dopamine