Kinetics of haemolytic activity in Proteus strains.
Proteus species produces toxins and constitutes a causative agent of some chronic and recurrent infections. For the study of haemolytic activity and the production and inhibition kinetics, a total of 140 local isolates were diagnosed and examined by the general biochemical methods, and their ability of haemolysis were tested by both direct and indirect methods utilizing the enrichment procedure for all strains. Two antibiotics, erythromycin and keflex (cephalexin), were tested for the study of haemolysis inhibition and its kinetisc. Rof further study, examples of Proteus species were selected; the new approach was based on mixing procedure between P. aeruginosa (also pyocyanine) and Proteus species for inhibition of haemolytic activity. Spectrophotometric analysis were used parallel to these studies to support quantitatively the observed results as all samples show an absorption centre at 542 +/- 1 nm. Results of such analysis of haemolytic activity and inhibition kinetics are presented.
Kandela
S A
SA
Saddam College of Medicine, Baghdad, Iraq.
al-Shibib
A S
AS
D002506:Cephalexin / Q000494:pharmacology; D004917:Erythromycin / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000096:biosynthesis; D006461:Hemolysis; D011511:Proteus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013053:Spectrophotometry
D006460:Hemolysin Proteins; D004917:Erythromycin; D002506:Cephalexin