Studies on monoclonal antibody against recombinant human granulocyte colony-stimulating factor.
Three hybridoma cell lines producing monoclonal antibodies (McAb) against recombinant human granulocyte colony-stimulating factor (rhG-CSF) have been established by fusing mouse myeloma cell line SP2/0 cells with spleen cells from a BALB/c mouse immunized with rhG-CSF. Ascites was obtained from BALB/c mice by injecting the hybridoma cells intraperitoneally. Three McAbs were purified by the caprylic acid-ammonium sulfate method. For each, the IgG subclass, valence and activity, molecular weight, specificity and affinity were determined. The applications of McAbs against rhG-CSF in the clinic and laboratory are discussed.
Guo
X
X
PUMC Hospital, CAMS, Beijing.
Wang
A
A
Li
X
X
Guo
Z
Z
Zhang
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002459:Cell Fusion; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000276:immunology*; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000473:pathology; D011994:Recombinant Proteins / Q000276:immunology; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor