Quantitative determination of the intracellular concentration of the various forms of HPr, a phosphocarrier protein of the phosphoenolpyruvate: sugar phosphotransferase system in growing cells of oral streptococci.
A simple procedure for quantitative estimation of the different phosphorylated forms of the phosphocarrier protein HPr in growing cells of oral streptococci is described. The growth of the cells was rapidly stopped by acidification of the medium and concomitant addition of the ionophore Gramicidin D. This procedure inactivated Enzyme I, HPr(Ser) kinase, HPr(Ser-P) phosphatase, and the enzymes involved in the metabolism of the allosteric effectors as well as the substrates of HPr phosphorylation. The cellular concentrations of HPr (His approximately P), HPr (Ser-P), HPr (His approximately P) (Ser-P), and free HPr were then determined by crossed immunoelectrophoresis.
Vadeboncoeur
C
C
Groupe de recherche en écologie buccale (GREB), Department of Biochemistry (Sciences) School of Dental Medicine, Université Laval, Ste-Foy, Quebec.
Brochu
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D
Reizer
J
J
D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D006096:Gramicidin / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D008722:Methods; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000032:analysis* / Q000378:metabolism; D010766:Phosphorylation; D013291:Streptococcus / Q000032:analysis* / Q000201:enzymology; D013295:Streptococcus mutans / Q000032:analysis* / Q000201:enzymology
D001426:Bacterial Proteins; D006096:Gramicidin; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr