Granular cell brain tumors of the laboratory rat: an immunohistochemical approach.
We have studied paraffin-embedded specimens of 17 rat granular cell brain tumors (GCBT) from four long-term drug safety carcinogenicity studies by peroxidase-antiperoxidase (PAP) immunohistochemistry with either polyvalent or monoclonal antibodies against glial fibrillary acidic protein (GFAP), S-100 protein (S-100), Leu-7 epitopes, vimentin (VIM), keratin, desmin, and myelin basic protein. We have found that 9 of the 17 GCBT contained GFAP-positive, S-100-positive, and VIM-positive astrocytes, while GFAP-positive and VIM-positive granular cells were observed in 5 of these 9 tumors. Our findings indicate that astroglial cells are involved in rat GCBT and suggest that an astrocytic origin should be considered for these neoplasms.
Perentes
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Sandoz Pharma Ltd., Department of Toxicology, Basle, Switzerland.
Maraziotis
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Qureshi
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SR
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis; D001932:Brain Neoplasms / Q000473:pathology*; D018959:CD57 Antigens; D003893:Desmin / Q000032:analysis; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D016586:Granular Cell Tumor / Q000473:pathology*; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008297:Male; D004676:Myelin Basic Protein / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000032:analysis; D014746:Vimentin / Q000032:analysis
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018959:CD57 Antigens; D003893:Desmin; D005904:Glial Fibrillary Acidic Protein; D004676:Myelin Basic Protein; D009418:S100 Proteins; D014746:Vimentin; D007633:Keratins