Expression of activin A/erythroid differentiation factor in murine bone marrow stromal cells.
Accumulating evidence suggests that activin A/erythroid differentiation factor (EDF), a homodimer of beta A chain, is a physiologic hematopoietic factor, particularly of erythroid lineage. Media conditioned by phorbol myristate acetate (PMA)-stimulated murine bone marrow stromal cell lines, MC3T3-G2/PA6 and ST2, contained activin A/EDF, assayed as the activity inducing erythroid differentiation of an activin A/EDF-responsive murine erythroleukemia cell clone F5. Follistatin, a protein specifically binding to activin A/EDF, abolished this erythroid differentiation. Northern blot analysis showed that PMA rapidly increased beta A chain messenger RNA levels in MC3T3-G2/PA6 and ST2 cells. In a search for natural stimulators, we found that tumor necrosis factor-alpha, in itself and synergistically with interleukin-1 beta, induced activin A/EDF production in both cell lines. These results indicate that stromal cells produce activin A/EDF in bone marrow.
Yamashita
T
T
Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Takahashi
S
S
Ogata
E
E
D028341:Activins; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D004357:Drug Synergism; D015672:Erythroid Precursor Cells / Q000166:cytology; D015870:Gene Expression; D007265:Inhibins / Q000096:biosynthesis / Q000235:genetics*; D007375:Interleukin-1 / Q000494:pharmacology; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D007375:Interleukin-1; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D028341:Activins; D007265:Inhibins; D013755:Tetradecanoylphorbol Acetate