[Effects of NIK-228 on gastric acid secretion in rats using the congo red sprayed method].
We have reported the antiulcer activities of a new compound that we named NIK-228 (3-hydroxy-methyl-2-methylimidazo [2, 1-b] benzothiazole). In the present report, we studied the antisecretory effects of NIK-228 on basal and stimulated gastric acid secretion using the Congo red sprayed method. Male Wistar rats (200 to 250 g) were used after 24 hr of fasting (without water). NIK-228, atropine and cimetidine were administered orally or intravenously 1 hr before operation for Congo red spraying. NIK-228 (100 mg/kg, p.o.), atropine (5 mg/kg p.o.) and cimetidine (100 mg/kg, p.o.) all inhibited basal gastric acid secretion. Oral administration of NIK-228 and atropine inhibited gastrin, 2-deoxy-D-glucose (2-DG) and bethanechol-induced acid secretion, but didn't inhibit histamine-induced acid secretion. Cimetidine inhibited all of histamine, gastrin, 2-DG and bethanechol-induced acid secretion. In vagotomized rats, oral and intravenous administration of atropine both inhibited bethanechol-induced acid secretion, but NIK-228 was not inhibited. These results suggested that antisecretory effects of NIK-228 were caused by the central vagal systems.
Kagoshima
M
M
Department of Clinical Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Hoshino
R
R
Shimada
H
H
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D052160:Benzothiazoles; D018723:Bethanechol; D001626:Bethanechol Compounds / Q000037:antagonists & inhibitors; D002927:Cimetidine / Q000494:pharmacology; D003224:Congo Red; D003847:Deoxyglucose / Q000037:antagonists & inhibitors; D003864:Depression, Chemical; D005744:Gastric Acid / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling / Q000379:methods; D013844:Thiazoles / Q000494:pharmacology*; D014630:Vagus Nerve / Q000502:physiology
D052160:Benzothiazoles; D001626:Bethanechol Compounds; D007093:Imidazoles; D013844:Thiazoles; D018723:Bethanechol; C070560:NIK 228; D003224:Congo Red; D001285:Atropine; D002927:Cimetidine; D003847:Deoxyglucose