title: Characterization of two developmentally regulated sea urchin U2 small nuclear RNA promoters: a common required TATA sequence and independent proximal and distal elements.
abstract: The promoters of two U2 small nuclear RNA genes isolated from the sea urchin Lytechinus variegatus were mapped by microinjection of genes into sea urchin zygotes. One gene, LvU2E, is expressed only in oocytes and embryos and is found in a tandemly repeated gene set, while the other gene, LvU2L, is a single-copy gene and is expressed in embryos and somatic cells. The promoters each contain a TATA sequence at -25 which is required for expression, a proximal sequence element (PSE) centered at -55 required for expression, a sequence at -100 which couples the core promoter (PSE plus TATA box) to the upstream element, and an upstream sequence which stimulates expression fourfold. The PSE together with the TATA sequence is sufficient to determine the transcription start site. There is no sequence similarity between the -100 and PSE sequences of the two genes. The -100 sequences can be interchanged between the two genes. The LvU2E PSE functions in the context of the LvU2L gene, but the LvU2L PSE functions poorly in the context of the LvU2E gene.
authors.affiliation: Department of Chemistry, Florida State University, Tallahassee 32306.
authors.forename: B
authors.initials: B
authors.lastname: Stefanovic
authors.forename: W F
authors.initials: WF
authors.lastname: Marzluff
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004252:DNA Mutational Analysis; D005786:Gene Expression Regulation / Q000235:genetics*; D008845:Microinjections; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012342:RNA, Small Nuclear / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012617:Sea Urchins / Q000235:genetics*; D016385:TATA Box / Q000235:genetics
chemical_list: D012342:RNA, Small Nuclear