Light and electron microscopic analysis of projection neurons retrogradely labeled with Fluoro-Gold: notes on the application of antibodies to Fluoro-Gold.
Fluoro-Gold has been a very popular fluorescent tracer used recently for retrogradely labeling projection neurons. In this study we described the advantages of using antibodies to Fluoro-Gold in conventional immunohistochemical reaction protocols to further extend the usefulness of this tracer for both light and electron microscopic neuroanatomical studies.
Chang
H T
HT
Department of Anatomy and Neurobiology, College of Medicine, University of Tennessee, Memphis 38163.
Kuo
H
H
Whittaker
J A
JA
Cooper
N G
NG
D000818:Animals; D001370:Axonal Transport; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology / Q000201:enzymology; D002795:Choline O-Acetyltransferase / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods; D009474:Neurons / Q000166:cytology* / Q000201:enzymology; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines; D002795:Choline O-Acetyltransferase