title: Antigen specificity of anticolipase monoclonal antibodies: characterization of colipase-Fab complexes using gel filtration high-performance liquid chromatography.
abstract: The epitope specificity of eight mouse monoclonal antiporcine procolipase antibodies (MAbs) was characterized on the basis of their competitive binding with antigen. Binary and ternary Fab-colipase complexes formed between antibody and porcine procolipase or its trypsin activated derivative were identified using gel filtration HPLC. The eight MAbs were divided in two groups that recognized overlapping epitopes located in distinct antigenic regions on procolipase. The gel filtration HPLC technique allowed to characterize two MAbs which did not react with solid-phase coupled antigen. Three MAbs formed Fab-antigen complexes with procolipase and not with activated colipase which suggests that epitopes recognized by these MAbs involve residues of the N-terminal pentapeptide of procolipase.
authors.affiliation: Institut de Chimie Biologique, Faculté St Charles Place Victor-Hugo, Marseille, France.
authors.forename: N
authors.initials: N
authors.lastname: Rugani
authors.forename: C
authors.initials: C
authors.lastname: Dezan
authors.forename: B
authors.initials: B
authors.lastname: Bellon
authors.forename: L
authors.initials: L
authors.lastname: Sarda
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001667:Binding, Competitive; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003089:Colipases / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D013552:Swine; D014357:Trypsin / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D003089:Colipases; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D014357:Trypsin