Analysis of the mechanism of graft-versus-host-like disease in B6 mice with transferred B6-lpr spleen cells.
Irradiated C57BL/6(B6) mice, when they were injected with spleen cells of C57BL/6J-lpr/lpr(B6-lpr) mice, developed splenomegaly at 2 weeks post-transfer, but afterward displaced by GVH-like disease. At 2 weeks the enlarged spleen in the chimeric mice, designated as [B6-lpr----B6] chimera, contained about 70% of the total cell population as CD8-positive T cells. Spleen cells from [B6-lpr----B6] chimeras were unresponsive to Con A and LPS stimulation and suppressed the mitogenic response of B6, B6-lpr, and C3H spleen cells to Con A. However, they had no cytotoxic activity towards Con A blasts of B6 and B6-lpr spleen cells. The suppressor activity found in the [B6-lpr----B6] spleen cells was removed by pretreatment of them with anti-Thy-1.2 or anti-CD8(Lyt2.2) plus complement. The present experiment showed that enormous proliferation of CD8-positive suppressor T cells was induced in the [B6-lpr----B6] chimeras. These cells were probably responsible for the GVH-like lymphoid atrophy observed in these [B6-lpr----B6] chimeras.
Matsunaga
T
T
Section of Immunopathogenesis, Hokkaido University, Sapporo, Japan.
Kobayashi
S
S
Harada
H
H
Kawaguchi
Y
Y
Wang
W
W
Okuyama
H
H
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis; D015704:CD4 Antigens / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D003165:Complement System Proteins / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000276:immunology*; D007108:Immune Tolerance; D007376:Interleukin-2 / Q000096:biosynthesis; D017493:Leukocyte Common Antigens; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011828:Radiation Chimera; D013154:Spleen / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D015704:CD4 Antigens; D016827:CD8 Antigens; D007376:Interleukin-2; D008070:Lipopolysaccharides; D018800:Thy-1 Antigens; D003208:Concanavalin A; D003165:Complement System Proteins; D017493:Leukocyte Common Antigens