Sequence and expression of the Escherichia coli K1 neuC gene product.
The nucleotide sequence of the neuC gene of the Escherichia coli K1 capsule gene cluster encodes a protein with a predicted molecular weight of 44,210 containing 391 amino acids. A chimeric protein with beta-galactosidase fused to the carboxy terminus of the neuC gene product (P7) was constructed and purified. Its amino-terminal sequence confirmed the prediction from the nucleotide sequence that the neuC gene overlaps the distal end of the neuA gene by a single base pair. Both the neuA and neuC genes are coexpressed under the control of a single upstream T7 or tac promoter, suggesting that neuA and neuC are part of an operon.
Zapata
G
G
Laboratory of Bacterial Polysaccharides, Center for Biologics Evaluation and Research, Bethesda, Maryland 20892.
Crowley
J M
JM
Vann
W F
WF
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D016667:Bacterial Capsules / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D000216:N-Acylneuraminate Cytidylyltransferase / Q000235:genetics; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins; D012794:Sialic Acids / Q000235:genetics*
D000942:Antigens, Bacterial; D000954:Antigens, Surface; C030659:K antigens; D011993:Recombinant Fusion Proteins; D012794:Sialic Acids; D000216:N-Acylneuraminate Cytidylyltransferase; D019158:N-Acetylneuraminic Acid