Macrophages and smooth muscle cells express lipoprotein lipase in human and rabbit atherosclerotic lesions.
Lipoprotein lipase (LPL; EC 3.1.1.34) may promote atherogenesis by producing remnant lipoproteins on the endothelial surface and by acting on lipoproteins in the artery wall. In vitro, smooth muscle cells and macrophages synthesize LPL, but in human carotid lesions only a few smooth muscle cells were reported to contain LPL protein. Endothelial cells do not synthesize LPL in vitro, but in normal arteries intense immunostaining for LPL is present on the endothelium. We used Northern blot analysis, in situ hybridization, and immunocytochemistry of human and rabbit arteries to determine cellular distribution and the site of the synthesis of LPL in atherosclerotic lesions. Northern blot analysis showed that LPL mRNA was detectable in macrophage-derived foam cells isolated from arterial lesions of "ballooned" cholesterol-fed rabbits. In situ hybridization studies of atherosclerotic lesions with an antisense riboprobe showed a strong hybridization signal for LPL mRNA in some, but not all, lesion macrophages, which were mostly located in the subendothelial and edge areas of the lesions. Also, some smooth muscle cells in lesion areas also expressed LPL mRNA. Immunocytochemistry of frozen sections of rabbit lesions with a monoclonal antibody to human milk LPL showed intense staining for LPL protein in macrophage-rich intimal lesions. The results suggest that lesion macrophages and macrophage-derived foam cells express LPL mRNA and protein. Some smooth muscle cells in the lesion areas also synthesize LPL. These data are consistent with an important role for LPL in atherogenesis.
Ylä-Herttuala
S
S
Department of Medicine, University of California, San Diego, La Jolla 92093-0613.
Lipton
B A
BA
Rosenfeld
M E
ME
Goldberg
I J
IJ
Steinberg
D
D
Witztum
J L
JL
D000818:Animals; D016375:Antisense Elements (Genetics); D001011:Aorta / Q000201:enzymology* / Q000473:pathology; D001013:Aorta, Thoracic / Q000201:enzymology; D001161:Arteriosclerosis / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D015152:Blotting, Northern; D002791:Cholesterol, Dietary; D003001:Cloning, Molecular; D015342:DNA Probes; D004036:Diet, Atherogenic; D005487:Foam Cells / Q000201:enzymology; D006801:Humans; D006937:Hypercholesterolemia / Q000201:enzymology; D006949:Hyperlipidemias / Q000201:enzymology / Q000235:genetics; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000201:enzymology*; D016676:Macrophages, Alveolar; D009131:Muscle, Smooth, Vascular / Q000201:enzymology* / Q000473:pathology; D015345:Oligonucleotide Probes; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011817:Rabbits
D016375:Antisense Elements (Genetics); D002791:Cholesterol, Dietary; D015342:DNA Probes; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012313:RNA; D008071:Lipoprotein Lipase