title: [The detection of the Marburg virus antigen by solid-phase immunoenzyme analysis].
abstract: Comparative studies of two variants of the enzyme-linked immunosorbent assay (ELISA) were carried out to determine the sensitivity of the detection of Marburg virus antigens in Vero cells. Both competitive and two-antibody ELISA variants detected as little as 5 ng of Marburg virus antigen. The Vero cell monolayer was found to produce 5-50 ng/0.05 ml of the virus-specific proteins at 6 to 8 days postinfection.
authors.forename: A S
authors.initials: AS
authors.lastname: Vladyko
authors.forename: A A
authors.initials: AA
authors.lastname: Chepurnov
authors.forename: S I
authors.initials: SI
authors.lastname: Bystrova
authors.forename: N N
authors.initials: NN
authors.lastname: Lemeshko
authors.forename: I S
authors.initials: IS
authors.lastname: Lukashevich
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000097:blood* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D000939:Epitopes / Q000097:blood / Q000302:isolation & purification; D006168:Guinea Pigs; D007114:Immunization; D029024:Marburgvirus / Q000276:immunology*; D011817:Rabbits; D012680:Sensitivity and Specificity; D012692:Serial Passage; D013997:Time Factors; D014709:Vero Cells
chemical_list: D000956:Antigens, Viral; D000939:Epitopes
vernacular_title: Vyiavlenie antigena virusa Marburg metodom tverdofaznogo immunofermentnogo analiza.