title: Effect of anti-carbohydrate antibodies on HIV infection in a monocytic cell line (U937).
abstract: Monoclonal antibodies (mAbs) against carbohydrate epitopes of gp120 have recently been found to inhibit HIV infection of lymphocytes in vitro thereby opening new possibilities for vaccine considerations. Antibody-dependent enhancement of infection has however come increasingly into focus. This study therefore investigated the neutralization of HIV in a monocytic cell line (U937) using mAbs against these carbohydrate gp120-epitopes. While antibodies against one of the epitopes (AI) neutralized infection of U937 cells despite binding to the Fc-receptor, one mAb against the sialosyl-Tn epitope enhanced infection. This enhancement was independent of complement and could be blocked by mAb Leu3a against the CD4-receptor. The study indicated that enhancement of infection in monocytic cells can occur by the same anti-carbohydrate antibodies that neutralize infection in lymphocytes, and that antibody mediated enhancement may depend on location of the epitope on gp120 rather than whether the antibody binds Fc-receptors.
authors.affiliation: Department of Infectious Diseases, Hvidovre Hospital, Denmark.
authors.forename: J E
authors.initials: JE
authors.lastname: Hansen
authors.forename: C
authors.initials: C
authors.lastname: Nielsen
authors.forename: H
authors.initials: H
authors.lastname: Clausen
authors.forename: L R
authors.initials: LR
authors.lastname: Mathiesen
authors.forename: J O
authors.initials: JO
authors.lastname: Nielsen
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000276:immunology*; D002460:Cell Line; D003165:Complement System Proteins / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000382:microbiology*; D011961:Receptors, Fc / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D002241:Carbohydrates; D000939:Epitopes; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120; D011961:Receptors, Fc; D003165:Complement System Proteins