DNA repair and mutant frequency in schizophrenia.
The in vivo frequency of mutants resulting from mutation at the hprt locus in human T-lymphocytes was determined with a cloning assay. T-lymphocytes were obtained from 14 individuals diagnosed with schizophrenia and 5 controls. No significant difference in mutant frequency was observed between the 2 groups. In addition, DNA-repair capacity was measured with the unscheduled DNA synthesis technique in lymphocytes from 7 individuals diagnosed with schizophrenia and 7 controls. Repair capacity was determined following treatment with MMS, MNNG, and 20 J/m2 ultraviolet light. No significant differences in DNA repair were observed between the patient and control groups in response to any of the 3 DNA-damaging agents. These results argue against differences between normal and schizophrenic individuals with respect to in vivo mutant frequency or their capacity to repair DNA lesions induced by MMS, MNNG, or ultraviolet radiation.
Magin
G K
GK
Genetics Laboratory, Vermont Regional Cancer Center, University of Vermont, Burlington 05401.
Robison
S H
SH
Breslin
N
N
Wyatt
R J
RJ
Alexander
R C
RC
D003001:Cloning, Molecular; D004260:DNA Repair; D004261:DNA Replication; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D009154:Mutation; D012016:Reference Values; D012559:Schizophrenia / Q000201:enzymology / Q000235:genetics*; D013601:T-Lymphocytes / Q000201:enzymology
D007041:Hypoxanthine Phosphoribosyltransferase