title: [Monoclonal antibodies obtained against G-protein epitopes: comparison of immunoreactions seen in the brain, retina and striated muscular tissue].
abstract: Monoclonal antibodies have been obtained against a purified fraction of brain G proteins containing the Gi alpha, G0 alpha, G beta, and G gamma subunits. After characterization, two monoclonal antibodies have been used to detect the cellular distribution of the two epitopes in neural, retinal and muscular tissues: ELISA, cross-dot and Western blot demonstrated that F.IV.5 is an anti-G beta antibody specific for the 36 kDa beta-subunit. ELISA, cross-dot and immunocytochemical distribution of the epitopes recognized by F.VII.9 suggested that this antibody recognizes epitopes which are also detected with polyclonal anti-G0 alpha antibodies. With both monoclonal antibodies, we confirmed that G proteins demonstrated a sub-membranous distribution as well as extensively cytoplasmic, axoplasmic or sarcoplasmic distributions in different cell types.
authors.affiliation: Laboratoire de Neurobiologie endocrinologique, U.R.A. n 1197, C.N.R.S., Université de Montpellier-II.
authors.forename: S
authors.initials: S
authors.lastname: Peraldi-Roux
authors.forename: P
authors.initials: P
authors.lastname: Mangeat
authors.forename: B
authors.initials: B
authors.lastname: Rouot
authors.forename: J
authors.initials: J
authors.lastname: Oliver
authors.forename: S
authors.initials: S
authors.lastname: Herbute
authors.forename: P
authors.initials: P
authors.lastname: Brabet
authors.forename: V
authors.initials: V
authors.lastname: Homburger
authors.forename: M
authors.initials: M
authors.lastname: Toutant
authors.forename: J
authors.initials: J
authors.lastname: Bockaert
authors.forename: J
authors.initials: J
authors.lastname: Gabrion
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000276:immunology*; D009412:Nerve Fibers / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000276:immunology*; D012160:Retina / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; C029152:G-substrate; D009419:Nerve Tissue Proteins
vernacular_title: Obtention d'anticorps monoclonaux dirigés contre des épitopes de protéines G: comparaison des immunoréactions observées dans le cerveau, la rétine et le tissu musculaire strié.