Expression of intercellular adhesion molecule 1 (ICAM-1) on human articular cartilage chondrocytes.
Monoclonal antibodies have been used to demonstrate the induction of intercellular adhesion molecule 1 (ICAM-1) on chondrocytes in human articular cartilage. ICAM-1 was found not to be constitutively expressed but could be induced by exogenous interleukin 1 alpha(IL1- alpha) at concentrations ranging from 0.01 to 20 ng/ml during in vitro culture. Maximum expression was observed with 2-5ng/ml. In time-course experiments ICAM-1 was not expressed after 4h in culture with IL1 alpha. Expression was induced by 16h and was sustained for a minimum of 6 days in the continued presence of the cytokine. The endothelial leukocyte adhesion molecule (ELAM-1) was not expressed on chondrocytes and was not induced by IL1-alpha.
Davies
M E
ME
Strangeways Research Laboratory, Worts Causeway, Cambridge, U.K.
Dingle
J T
JT
Pigott
R
R
Power
C
C
Sharma
H
H
D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D019040:E-Selectin; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1; D007375:Interleukin-1 / Q000494:pharmacology; D012016:Reference Values
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007375:Interleukin-1; D018799:Intercellular Adhesion Molecule-1