Stress protein synthesis by crayfish CNS tissue in vitro.
Some crustacean axons remain functional for months after injury. This unusual property may require stress proteins synthesized by those neurons or provided to them by glial cells. To begin to explore this hypothesis, we examined the conditions that stimulated stress protein synthesis by crayfish CNS tissue in vitro. Incubation for 1-15 h with arsenite or at temperatures about 15 degrees C higher than the acclimation temperature of 20 degrees C induced transient expression of several stress proteins. The heat stress response was blocked by Actinomycin D, suggesting that synthesis of new mRNA was required. In addition, the major crayfish 66 kD stress protein and its mRNA had sequence identities with the 70 kD stress proteins of mammals. Since the crayfish stress response has much in common with that of other organisms, the unique advantages of the crayfish nervous system can be used to study the impact of stress proteins on glial and neuronal function.
Rochelle
J M
JM
Zoology Dept., North Carolina State University, Raleigh 27695-7617.
Grossfeld
R M
RM
Bunting
D L
DL
Tytell
M
M
Dwyer
B E
BE
Xue
Z Y
ZY
D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D003400:Astacoidea / Q000378:metabolism*; D002490:Central Nervous System / Q000187:drug effects / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D015151:Immunoblotting; D066298:In Vitro Techniques; D008297:Male; D008322:Mammals / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature
D018053:Arsenites; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D003609:Dactinomycin; D012313:RNA; C015001:arsenite; D001151:Arsenic