title: Antisera raised against recombinant soluble CD4s can have reactivity to recombinant HIV-1 envelope glycoproteins.
abstract: Antisera from sheep immunized with two different recombinant soluble CD4 preparations both exhibited cross-reactive antibodies specific for recombinant gp120s and gp160s prepared in different expression systems in immunoblots and radiobinding assays. The cross-reactivity was broadly mapped to the amino-terminal 70K cleavage fragment of gp120 and more closely identified to residues 204-274 using a series of truncated recombinant gp120s. However, this reactivity could not be detected against virally encoded gp160/120.
authors.affiliation: Division of Immunobiology, National Institute for Biological Standards and Control, Potters Bar, Herts, UK.
authors.forename: M
authors.initials: M
authors.lastname: Page
authors.forename: V R
authors.initials: VR
authors.lastname: Patel
authors.forename: P
authors.initials: P
authors.lastname: Dilger
authors.forename: C R
authors.initials: CR
authors.lastname: Ling
authors.forename: R
authors.initials: R
authors.lastname: Thorpe
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D015686:Gene Products, env / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D019302:HIV Envelope Protein gp160; D007106:Immune Sera / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D011498:Protein Precursors / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D012756:Sheep / Q000276:immunology
chemical_list: D015704:CD4 Antigens; D000939:Epitopes; D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D007106:Immune Sera; D010446:Peptide Fragments; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins