Extracellular phospholipase A2 and histamine release from rat peritoneal mast cells.
Phospholipase A2 (PLA2) from cobra (Naja naja) venom and PLA2 from porcine pancreas accelerated IgE antibody-mediated histamine release from rat peritoneal mast cells. These enhancements were clearly abrogated by heating the enzymes and pretreatment with parabromophenacyl bromide, mepacrine and antiflammin. Indomethacin (cyclooxygenase inhibitor) and AA-861 (lipoxygenase inhibitor) did not affect the enhancement by PLA2. These results indicate that extracellular PLA2 enhances the IgE antibody-mediated histamine release from rat peritoneal mast cells without the participation of arachidonate.
Nagai
H
H
Department of Pharmacology, Gifu Pharmaceutical University, Japan.
Sakamoto
T
T
Kondo
M
M
Miura
T
T
Inagaki
N
N
Koda
A
A
D000818:Animals; D006636:Histamine Release / Q000187:drug effects / Q000502:physiology*; D007073:Immunoglobulin E / Q000502:physiology; D008297:Male; D008407:Mast Cells / Q000201:enzymology*; D010529:Peritoneal Cavity / Q000166:cytology; D010741:Phospholipases A / Q000378:metabolism* / Q000494:pharmacology; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains
D007073:Immunoglobulin E; D010741:Phospholipases A; D054467:Phospholipases A2