title: Characterization by somatic cell genetics of a monoclonal antibody to the MDR1 gene product (P-glycoprotein): determination of P-glycoprotein expression in multi-drug-resistant KB and CEM cell variants.
abstract: We isolated an IgG2a murine monoclonal antibody (MAb) termed MAb57, specifically reactive with multi-drug-resistant (MDR) human cells. Its specificity toward the MDRI gene product (P-glycoprotein) has been demonstrated by the concordant segregation of the MAb57 epitope with the MDRI gene in interspecific mouse x human cell hybrids, and the reactivity of several different MDRI gene-expressing cells with MAb57, particularly insect cells acutely infected with a baculovirus encoding the MDRI gene. MAb57 can be used to detect, by flow cytometry, variations in the relative drug-resistance levels of several MDR KB and CEM cell variants. This immunological probe has also proven useful in selectively destroying MDR target cells in an antibody-dependent cell-mediated (ADCC) assay system as well as in detecting P-glycoprotein expression in normal and malignant tissues and cells.
authors.affiliation: Laboratorio di Immunologia, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Cenciarelli
authors.forename: S J
authors.initials: SJ
authors.lastname: Currier
authors.forename: M C
authors.initials: MC
authors.lastname: Willingham
authors.forename: F
authors.initials: F
authors.lastname: Thiebaut
authors.forename: U A
authors.initials: UA
authors.lastname: Germann
authors.forename: A V
authors.initials: AV
authors.lastname: Rutherford
authors.forename: M M
authors.initials: MM
authors.lastname: Gottesman
authors.forename: S
authors.initials: S
authors.lastname: Barca
authors.forename: M
authors.initials: M
authors.lastname: Tombesi
authors.forename: S
authors.initials: S
authors.lastname: Morrone
authors.forename: A
authors.initials: A
authors.lastname: Santoni
authors.forename: M
authors.initials: M
authors.lastname: Mariani
authors.forename: C
authors.initials: C
authors.lastname: Ramoni
authors.forename: M L
authors.initials: ML
authors.lastname: Dupuis
authors.forename: M
authors.initials: M
authors.lastname: Cianfriglia
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002460:Cell Line; D004351:Drug Resistance; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007624:KB Cells; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D000939:Epitopes; D008562:Membrane Glycoproteins