title: Distribution and contents of free O-phosphoamino acids in animal tissues.
abstract: This study was carried out to investigate the distribution and contents of O-phosphoserine (P-Ser), O-phosphothreonine (P-Thr), and O-phosphotyrosine (P-Tyr) as their free forms in animal tissues. After extraction of a tissue sample with trichloroacetic acid, these O-phosphoamino acids were converted into their N-isobutoxycarbonyl trimethyl ester derivatives and then quantitated by GLC, with flame photometric detection, on a DB-1701 capillary column. Using this method, nanogram levels of O-phosphoamino acids in tissue samples could be accurately and precisely determined without any interference by coexisting substances. Free P-Ser and P-Thr were widely found in animal tissues, pancreas, spleen, stomach, kidney, liver, and lung containing considerable amounts of these O-phosphoamino acids. On the other hand, free P-Tyr was not detected in any of the tissues investigated in this study.
authors.affiliation: Faculty of Pharmaceutical Sciences, Okayama University.
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: N
authors.initials: N
authors.lastname: Sakiyama
authors.forename: M
authors.initials: M
authors.lastname: Makita
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002645:Chickens; D002849:Chromatography, Gas; D005399:Fishes; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009206:Myocardium / Q000737:chemistry; D009928:Organ Specificity; D010768:Phosphoserine / Q000032:analysis*; D010769:Phosphothreonine / Q000032:analysis*; D019000:Phosphotyrosine; D013045:Species Specificity; D013552:Swine; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D010769:Phosphothreonine; D010768:Phosphoserine; D019000:Phosphotyrosine; D014443:Tyrosine