Localizing DNA and RNA within nuclei and chromosomes by fluorescence in situ hybridization.
The enormous potential of in situ hybridization derives from the unique ability of this approach to directly couple cytological and molecular information. In recent years, there has been a surge of success in powerful new applications, resulting from methodologic advances that bring the practical capabilities of this technology closer to its theoretical potential. A major advance has been improvements that enable, with a high degree of reproducibility and efficiency, precise visualization of single sequences within individual metaphase and interphase cells. This has implications for gene mapping, the analysis of nuclear organization, clinical cytogenetics, virology, and studies of gene expression. This article discusses the current state of the art of fluorescence in situ hybridization, with emphasis on applications to human genetics, but including brief discussions on studies of nuclear DNA and RNA organization, and on applications to clinical genetics and virology. Although a review of all of the literature in this field is not possible here, many of the major contributions are summarized along with recent work from our laboratory.
McNeil
J A
JA
University of Massachusetts Medical School, Department of Cell Biology, Worcester 01655.
Johnson
C V
CV
Carter
K C
KC
Singer
R H
RH
Lawrence
J B
JB
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D004247:DNA / Q000032:analysis; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes; D012313:RNA / Q000032:analysis
D015341:Nucleic Acid Probes; D012313:RNA; D004247:DNA