Properties of an immunoglobulin binding factor from human seminal plasma.
A soluble factor (IBF) in human seminal plasma that binds serum immunoglobulins (Ig) of various species was purified to homogeneity by ammonium sulfate precipitation, preparative isoelectrofocusing, and gel filtration chromatography. The purified IBF interacted weakly with Fc and F(ab')2 fragments and not with Fab. It interacted with anti-Leu 11b and polyclonal anti-Fc gamma RIII antibodies, but not with other anti-Fc gamma R antibodies (32.2, IV.3 and 3G8). IBF is probably a non-glycosylated protein with isoelectric point ranging from 5.1 to 5.8. The estimated Mr of the purified native IBF is 27 kD, determined by sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) under non-reducing condition. In its native form, IBF did not bind Ig or interact with anti-Fc gamma R antibodies. Following SDS-PAGE under reducing condition, IBF migrated as a single protein with an estimated Mr of 16 kD and interacted with Ig of various species and with anti-Leu 11b antibodies. When carboxymethylated, however, IBF did not bind IgG. The present results suggest that free sulfhydryl groups of IBF is required for Ig binding.
Liang
Z G
ZG
Center for Biomedical Research, Population Council, New York, New York 10021.
Kamada
M
M
Koide
S S
SS
D000906:Antibodies / Q000276:immunology; D000943:Antigens, Differentiation / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D001665:Binding Sites; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007525:Isoelectric Focusing; D010084:Oxidation-Reduction; D011961:Receptors, Fc / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D017452:Receptors, IgG; D012661:Semen / Q000737:chemistry*; D013194:Staining and Labeling
D000906:Antibodies; D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG