An altered constitutive peptide in sym 5 mutants of Pisum sativum L.
Mutational analysis of Pisum sativum L. was used to search for constitutive proteins that might function in nodule formation. The sym 5 locus is a mutational hot spot, represented by seven independently derived mutant lines with decreased nodulation. Comparison of two-dimensional polyacrylamide gels of in vitro-translated root RNA showed a consistent difference in the migrational pattern of one peptide. In the nodulating parental cultivar 'Sparkle', a 66 kDa peptide had a pI of 5.9. In four of the five tested sym 5 mutants, the 66 kDa peptide had a more acidic pI of 5.8. This 66 kDa peptide is found in lateral root, tap root, and shoot. Its expression was independent of rhizobial inoculation, root temperature, or light.
Fearn
J C
JC
Boyce Thompson Institute for Plant Research, Ithaca, NY 14853-1801.
LaRue
T A
TA
D015180:Electrophoresis, Gel, Two-Dimensional; D007887:Fabaceae / Q000235:genetics*; D005808:Genes, Recessive; D008970:Molecular Weight; D009154:Mutation; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification; D010946:Plants, Medicinal; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012231:Rhizobium / Q000235:genetics; D013559:Symbiosis / Q000235:genetics*
D010940:Plant Proteins; D012313:RNA