title: Phosphorylation by calcium calmodulin-dependent protein kinase II and protein kinase C modulates the activity of nitric oxide synthase.
abstract: Nitric oxide synthase purified from rat brain, which is Ca2+ and calmodulin dependent, was phosphorylated by calcium calmodulin-dependent protein kinase II as well as protein kinase C. Phosphorylation by calcium calmodulin-dependent protein kinase II resulted in a marked decrease in enzyme activity (33% of control) without changing the co-factor requirements, whereas a moderate increase in enzyme activity (140% of control) was observed after phosphorylation by protein kinase C. These findings indicate that brain nitric oxide synthase activity may be regulated not only by Ca2+/calmodulin and several co-factors, but also by phosphorylation.
authors.affiliation: Abbott Laboratories, Abbott Park, Illinois 60064.
authors.forename: M
authors.initials: M
authors.lastname: Nakane
authors.forename: J
authors.initials: J
authors.lastname: Mitchell
authors.forename: U
authors.initials: U
authors.lastname: Förstermann
authors.forename: F
authors.initials: F
authors.lastname: Murad
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002850:Chromatography, Gel; D007700:Kinetics; D019001:Nitric Oxide Synthase; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity
chemical_list: D000255:Adenosine Triphosphate; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D011494:Protein Kinases; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases