title: Analysis of rat brain microdialysate by gas chromatography-high-resolution selected-ion monitoring mass spectrometry.
abstract: Gas chromatography-high-resolution selected-ion monitoring mass spectrometry was used to analyze catecholamine metabolites in rat brain microdialysate. Dialysate samples were collected in vials containing stable isotope analogues of homovanillic acid (HVA), 3-methoxy-4-hydroxyphenylglycol (MHPG) and 5-hydroxyindoleacetic acid (5HIAA) and analyzed as their trimethylsilyl derivatives. The metabolite levels were monitored at 20-min intervals throughout the time course of the experiment, beginning immediately after surgery and implantation of the dialysis probe and ending 4 h after amphetamine treatment. The levels of HVA were observed to decrease after amphetamine treatment, while those of MHPG and 5HIAA did not change significantly.
authors.affiliation: Burroughs Wellcome Co., Research Triangle Park, NC 27709.
authors.forename: S Y
authors.initials: SY
authors.lastname: Chang
authors.forename: T A
authors.initials: TA
authors.lastname: Moore
authors.forename: L L
authors.initials: LL
authors.lastname: Devaud
authors.forename: L C
authors.initials: LC
authors.lastname: Taylor
authors.forename: E B
authors.initials: EB
authors.lastname: Hollingsworth
mesh_terms: D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002395:Catecholamines / Q000032:analysis*; D003956:Dialysis; D008401:Gas Chromatography-Mass Spectrometry; D006719:Homovanillic Acid / Q000032:analysis; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002395:Catecholamines; D008734:Methoxyhydroxyphenylglycol; D006897:Hydroxyindoleacetic Acid; D000661:Amphetamine; D006719:Homovanillic Acid