Histamine-induced chloride channels in apical membrane of isolated rabbit parietal cells.
The electrical properties of the apical membrane of isolated rabbit parietal cells were studied using the patch-clamp technique. The apical membrane of the parietal cells plated on Matrigel and maintained in culture conditions was identified by lectin-binding studies. Cell-attached and excised inside-out patches from 10(-4) M cimetidine-treated parietal cells infrequently contained Cl- channels (9% of the patches). A single class of voltage-dependent outwardly rectifying Cl- channels with 24 +/- 1-pS conductance was observed in 75% of the patches from cells stimulated (acid secreting) by 10(-4) M histamine. Other anions passed through these channels with a permeability sequence of I- (1.2) greater than Br- (1.1) greater than or equal to Cl- (1.0) greater than NO3- (0.7) greater than SO4(2-) (0.1), but there was a very low permeability for Na+ or K+ (PCl-/PNa+ or PCl-/PK+ greater than 5). In inside-out patch configurations the Cl- channel was insensitive to Ba2+ and stilbene derivatives but was inhibited by diphenylamine-2-carboxylic acid in a manner characteristic of a reversible open-channel blocker. It is concluded that H2-receptor agonist stimulation of acid secretion by rabbit parietal cells activates Cl- channels in the apical cell membrane.
Saccomani
G
G
Department of Physiology and Biophysics, University of Alabama, Birmingham 35294.
Psarras
C G
CG
Smith
P R
PR
Kirk
K L
KL
Shoemaker
R L
RL
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000632:Aminopyrine; D000818:Animals; D002478:Cells, Cultured; D018118:Chloride Channels; D002712:Chlorides / Q000378:metabolism*; D002927:Cimetidine / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology*; D007473:Ion Channels / Q000502:physiology; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000187:drug effects / Q000502:physiology*; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure; D010295:Parietal Cells, Gastric / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D011817:Rabbits; D013267:Stilbenes / Q000494:pharmacology
D018118:Chloride Channels; D002712:Chlorides; D007473:Ion Channels; D008565:Membrane Proteins; D013267:Stilbenes; D000632:Aminopyrine; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C022306:4,4'-dinitro-2,2'-stilbenedisulfonic acid; D002927:Cimetidine; D006632:Histamine; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid