Eosin Y staining of proteins in polyacrylamide gels.
A staining method is described in which various proteins in polyacrylamide gels can be stained by using eosin Y. After a brief incubation of a polyacrylamide gel in an acidic solution of 1% eosin Y, various proteins, including human erythrocyte membrane sialoglycoproteins which are not detectable by Coomassie blue R-250 (CB), can be detected with a sensitivity of 10 ng protein. This is far more sensitive than CB staining and is comparable to the sensitivity of silver staining. In a Western blot, the antigenicity of an eosin Y stained protein is retained. In addition, proteins on an immunoblot sheet can be detected by eosin Y staining. The method described is rapid, sensitive, and reproducible with various proteins in polyacrylamide gels and has the added advantage of also staining sialoglycoproteins.
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Department of Anatomy and Cell Biology, Texas College of Osteopathic Medicine/University of North Texas, Fort Worth 76107.
Fan
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Wise
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D000180:Acrylic Resins; D000199:Actins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004801:Eosine Yellowish-(YS); D006801:Humans; D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate
D000180:Acrylic Resins; D000199:Actins; D011506:Proteins; D012394:Rosaniline Dyes; C016680:polyacrylamide gels; D012967:Sodium Dodecyl Sulfate; C024757:Coomassie brilliant blue R; D004801:Eosine Yellowish-(YS)