Partial characterization of human amniotic membrane interferon.
1. The molecular weight and the electrofocusing profile of human amniotic membrane interferon (IFN-AM) were determined. 2. When submitted to gel filtration, IFN-AM showed a single 26-28 kDa component; in polyacrylamide gel electrophoresis one component of 19,500. 3. In electrofocusing, IFN-AM displayed a heterogeneous profile with 5 to 7 peaks, but different from human alpha or beta IFNs. This heterogeneity was reduced by previous treatment of IFN-AM with neuraminidase. 4. IFN-AM is a sialoglycoprotein similar to human beta IFN in terms of antigenicity but different from it in electrofocusing profile.
Ferreira
P C
PC
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
Gonzaga
C
C
Kroon
E G
EG
Dos-Santos
J R
JR
Bicalho
H M
HM
Golgher
R R
RR
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005321:Extraembryonic Membranes / Q000378:metabolism*; D006801:Humans; D007372:Interferons / Q000737:chemistry*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010920:Placenta / Q000737:chemistry*
D007372:Interferons