Molecular cloning and expression in Escherichia coli K-12 of chromosomal genes determining the O antigen of an E. coli O2: K1 strain.
The rfb gene cluster which determines the biosynthesis of the O2 O antigen has been cloned from an Escherichia coli O2: K1 strain isolated from a case of septicaemia in chickens. The region required for expression of the O antigen in E. coli K-12 was localised to a 10.7 to 14.15-kb segment which was shown to be chromosomal in origin with a close linkage to the gnd and his genetic loci.
Neal
B L
BL
Department of Microbiology, University of Sydney, New South Wales, Australia.
Tsiolis
G C
GC
Heuzenroeder
M W
MW
Manning
P A
PA
Reeves
P R
PR
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D002645:Chickens / Q000382:microbiology; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D008070:Lipopolysaccharides / Q000032:analysis / Q000235:genetics*; D019081:O Antigens; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens