Rat monoclonal antibodies to nonoverlapping epitopes of human immunodeficiency virus type 1 gp120 block CD4 binding in vitro.
Monoclonal antibodies (MAbs) to a recombinant form of the envelope glycoprotein gp120 of human immunodeficiency virus type 1 (HIV-1 IIIB) were raised in rats and screened for their ability to block recombinant gp120 binding to recombinant, soluble CD4 (sCD4) in vitro. Four such MAbs were identified and characterised. Each MAb bound strongly to gp120 from eight widely divergent HIV-1 strains from the United States and Africa. Two MAbs were mapped to the fourth conserved (C4) region of gp120, whereas the other two recognised an as yet undefined, conformationally sensitive epitope. MAbs to the latter epitope were the more potent in blocking the gp120-sCD4 interaction. None of the MAbs, however, had potent neutralising activity.
Cordell
J
J
Institute of Cancer Research, Sutton, Surrey, United Kingdom.
Moore
J P
JP
Dean
C J
CJ
Klasse
P J
PJ
Weiss
R A
RA
McKeating
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001667:Binding, Competitive; D015153:Blotting, Western; D015704:CD4 Antigens / Q000276:immunology*; D016466:CHO Cells; D006224:Cricetinae; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology* / Q000502:physiology; D015497:HIV-1 / Q000276:immunology*; D007700:Kinetics; D009500:Neutralization Tests; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D015704:CD4 Antigens; D000939:Epitopes; D015699:HIV Envelope Protein gp120; D011994:Recombinant Proteins