[Preliminary results of the DGTI Workshop for evaluating the reactivity of monoclonal anti-D].
Within a working group of the German transfusion society, a workshop was conducted to evaluate the reactivity of monoclonal anti-D supernatants. During this evaluation 10,000 samples were tested, including 325 Du-samples, with 3-5 IgM type antibodies and 3-4 IgG type antibodies, which were selected to also find 'D-variants'. In general, the reactivity with D+ red blood cells was good, and specific in respect to D negative samples. Some samples have been found to have a different pattern of reactivity than the monoclonal antibodies used. Dependent on the method and on the antibody, in some studies up to 100% of Du samples could be detected by anti-D of the IgM type. Some data indicate that the reaction with Du samples is not dependent on titer. In addition, in separate studies the data show a correlation of reactivity in respect to the Rh-phenotype (CcDuee greater than ccDuEe).
Sonnenborn
H H
HH
Biotest AG, Forschungszentrum, Offenbach.
Darda
C
C
Ernst
M
M
Flegel
W A
WA
Helmbold
W
W
Kasulke
D
D
Kühnl
P
P
Mueller-Eckhardt
C
C
Northoff
H
H
Poschmann
A
A
D000911:Antibodies, Monoclonal; D005858:Germany; D006801:Humans; D007518:Isoantibodies; D018029:Rho(D) Immune Globulin; D012955:Societies, Medical
D000911:Antibodies, Monoclonal; D007518:Isoantibodies; C061961:RHO(D) antibody; D018029:Rho(D) Immune Globulin
Vorläufige Ergebnisse des DGTI Workshops zur Evaluierung der Reaktivität von monoklonalem Anti-D.