Simultaneous determination of isbufylline and its major metabolites in rabbit blood and urine by reversed-phase high-performance liquid chromatography.
A sensitive high-performance liquid chromatographic assay for isbufylline and its major metabolites in rabbit blood and urine is described. After extraction, samples were eluted by a linear reversed-phase gradient. Specimens obtained after intravenous administration of isbufylline to rabbits were analysed to identify and subsequently quantify the potential metabolites. Using the ultraviolet absorption trace on the recorder as a reference, elution fractions were collected and analysed by mass spectrometry with the direct inlet system and gas chromatography-mass spectrometry after derivatization. Seven metabolites were identified and another five quantified. The method is specific, accurate, reproducible and recommended for pharmacokinetic studies.
Celardo
A
A
Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, S. Maria Imbaro, Italy.
Dell'Elba
G
G
Frassanito
R
R
Benfenati
E
E
Bonati
M
M
D015056:1-Methyl-3-isobutylxanthine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000652:urine; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D010276:Parasympatholytics / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
D010276:Parasympatholytics; C058878:isbufylline; D015056:1-Methyl-3-isobutylxanthine