[Identification, isolation and physico-chemical properties of neurospecific alpha2-globulin].
In the immunization process of rabbits with the protein fraction of the water-salt extract from human brain partially and concentrated at 500-600 times was received antiserum revealed brain specific alpha 2-globulin that is not identical to the known cytoplasmatic brain specific antigens. This antigen has got electrophoretic mobility of alpha 2-globulins, molecular weight 90 +/- 10 kD and isoelectric point 4.1-5.4. Develop the procedure for purification of this antigen on the basis of the combination ion change, affinity, hydrophobic chromatography gel-filtration and isochromatofocusing.
Chekhonin
V P
VP
Koroteeva
E A
EA
Morozov
G V
GV
Makarov
A V
AV
D000510:Alpha-Globulins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D001923:Brain Chemistry; D011232:Chemical Precipitation; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D004589:Electrophoresis, Disc / Q000379:methods; D006801:Humans; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
D000510:Alpha-Globulins; D009419:Nerve Tissue Proteins
Identifikatsiia, vydelenie i fiziko-khimicheskie svoĭstva neĭrospetsificheskogo alpha2-globulina.