Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherichia coli K-12.
The rfb genes of Vibrio cholerae O1 (Ogawa serotype) were subcloned into a derivative of pBR322. This plasmid was transformed into several Escherichia coli K-12 mutant strains which produce an incomplete lipopolysaccharide (LPS)-core-oligosaccharide region. The data indicate that the V. cholerae O-antigen is assembled onto the E. coli LPS and that at least two glucoses are needed in the core in order to achieve a high level of production. These data are consistent with the reported presence of glucose in the V. cholerae LPS-core-oligosaccharide region.
Morona
R
R
Enterovax Limited, Adelaide, South Australia.
Brown
M H
MH
Yeadon
J
J
Heuzenroeder
M W
MW
Manning
P A
PA
D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D002240:Carbohydrate Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006384:Hemagglutination; D008070:Lipopolysaccharides / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D019081:O Antigens; D014734:Vibrio cholerae / Q000235:genetics*
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens; D005947:Glucose