title: Stimulation of fatty acid oxidation in myocytes by phosphodiesterase inhibitors and adenosine analogues.
abstract: The effect of various phosphodiesterase inhibitors, and adenosine analogues on palmitate oxidation, were studied in isolated rat myocytes. Enoximone, milrinone, and dipyridamole, at a concentration of 250 microM, stimulated palmitate oxidation by 78%, 40%, and 43%, respectively. The specific A1-agonist, N6-cyclopentyladenosine, increased palmitate oxidation by 56%, at a concentration of 250 microM. Moreover, the nucleoside transport inhibitor, S-(P-Nitrobenzyl-)6-thioinosine, increased palmitate oxidation by 40%, at a concentration of 100 microM. These data suggest that the stimulation of palmitate oxidation by enoximone and adenosine analogues may be mediated via the inhibition of the uptake and/or the oxidation of glucose in myocytes.
authors.affiliation: Glaxo Inc., Department of Pharmacology, Research Triangle Park, NC 27709.
authors.forename: S
authors.initials: S
authors.lastname: Abdel-Aleem
authors.forename: M
authors.initials: M
authors.lastname: Badr
authors.forename: C
authors.initials: C
authors.lastname: Frangakis
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000676:Amrinone / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D004176:Dipyridamole / Q000494:pharmacology; D017335:Enoximone; D006321:Heart / Q000187:drug effects; D007093:Imidazoles / Q000494:pharmacology; D020105:Milrinone; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D011688:Purinones / Q000494:pharmacology; D011724:Pyridazines / Q000494:pharmacology; D011728:Pyridones / Q000494:pharmacology; D051381:Rats
chemical_list: D007093:Imidazoles; D010169:Palmitic Acids; D010726:Phosphodiesterase Inhibitors; D011688:Purinones; D011724:Pyridazines; D011728:Pyridones; D019308:Palmitic Acid; D004176:Dipyridamole; C042528:4,5-dihydro-6-(4-(imidazol-1-yl)phenyl)-5-methyl-3(2H)-pyridazinone; D017335:Enoximone; C011145:zaprinast; D020105:Milrinone; D000676:Amrinone; D000241:Adenosine; D015056:1-Methyl-3-isobutylxanthine