Preparation of antibacterial and antitoxic Clostridium difficile sera.
Preparation of Clostridium difficile antibacterial and antitoxic sera is presented. Fifty one strains (72%) were typeable within Delmee scheme. Twenty strains (28%) belonged to new Polish serogroups designated 18, 27, 70, 71, 72, 88, 89 and NICH. Supernatants of all toxigenic Clostridium difficile strains were neutralized by gamma-globulin fraction of goat Clostridium difficile antitoxin in neutralization assay when it was performed on McCoy cell line. Only 8 toxigenic strains (21%) were positive in counterimmunoelectrophoresis.
Sokół-Leszczyńska
B
B
Department of Bacteriology and Immunology, Institute of Biostructure Medical Academy, Warsaw, Poland.
Martirossian
G
G
Meisel-Mikołajczyk
F
F
Mierzejewski
J
J
Matras
J
J
Lipnicki
D
D
Sadowski
W
W
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood*; D000992:Antitoxins / Q000096:biosynthesis / Q000097:blood*; D016360:Clostridioides difficile / Q000145:classification / Q000276:immunology*; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis; D006041:Goats; D007106:Immune Sera / Q000096:biosynthesis*; D011817:Rabbits; D012703:Serotyping
D000907:Antibodies, Bacterial; D000992:Antitoxins; D007106:Immune Sera