Altered expression of insulin receptor types A and B in the skeletal muscle of non-insulin-dependent diabetes mellitus patients.
The human insulin receptor exists in two isoforms, HIR-A and HIR-B, which are generated by alternative splicing of a primary gene transcript and differ by a 12-amino acid insertion sequence in the alpha-subunit. The two receptor isoforms bind insulin with different affinities and are differentially expressed in human tissues. We report here a tissue-specific alteration of the insulin receptor RNA splice pattern in non-insulin-dependent diabetes mellitus (NIDDM) patients. Whereas skeletal muscle of healthy individuals contains exclusively high-affinity HIR-A-encoding RNA, we consistently find low-affinity HIR-B RNA expression in NIDDM muscle tissue at levels similar to HIR-A.
Mosthaf
L
L
Max-Planck-Institut für Biochemie, Matinsried, Federal Republic of Germany.
Vogt
B
B
Häring
H U
HU
Ullrich
A
A
D000368:Aged; D004247:DNA / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009132:Muscles / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012326:RNA Splicing; D011972:Receptor, Insulin / Q000235:genetics*; D012016:Reference Values; D014158:Transcription, Genetic
D012313:RNA; D004247:DNA; D011972:Receptor, Insulin