Purification of mitogen-activated protein kinase from epidermal growth factor-treated 3T3-L1 fibroblasts.
Sturgill
T W
TW
Ray
L B
LB
Anderson
N G
NG
Erickson
A K
AK
D016475:3T3 Cells; D000818:Animals; D001345:Autoradiography / Q000379:methods; D002021:Buffers; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002846:Chromatography, Affinity / Q000379:methods; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D007202:Indicators and Reagents; D007700:Kinetics; D051379:Mice; D010761:Phosphorus Radioisotopes; D010768:Phosphoserine / Q000032:analysis; D010769:Phosphothreonine / Q000032:analysis; D019000:Phosphotyrosine; D011494:Protein Kinases / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
D002021:Buffers; D007202:Indicators and Reagents; D010761:Phosphorus Radioisotopes; D010769:Phosphothreonine; D010768:Phosphoserine; D019000:Phosphotyrosine; D014443:Tyrosine; D004815:Epidermal Growth Factor; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases