A single base pair deletion from the inactive octamer-like motif of the 7S K distal sequence element brings full functionality in vivo.
Octamer sequence elements were analyzed for their capacity to induce the 7S K "core" promoter in vivo. The U6 distal sequence element (DSE) which contains a consensus sequence octamer, was able to support efficient 7S K expression in vivo. In contrast, no such function could be attributed to the octamer-like element alone, which is present within the 7S K DSE. However, conversion of this octamer-like element (ATTTaGCAT) to the octamer consensus sequence ATTTGCAT generated a potent DSE, even in the absence of the CACCC box, which constitutes the major functional element of the 7S K DSE. Both the consensus and the octamer-like sequences revealed no cooperativity with the CACCC box. Together, these results demonstrate that the octamer-like element of the wild-type 7S K DSE is definitely not functional in vivo. Furthermore, our experiments indicate that in contrast to the RNA polymerase II-transcribed small nuclear RNA genes, in intact cells a single functional DSE motif is necessary and sufficient for maximal transcription by RNA polymerase III of the 7S K RNA gene.
Kleinert
H
H
Department of Biochemistry, Faculty of Chemistry, Ruhr-University, Bochum, Federal Republic of Germany.
Assert
R
R
Benecke
B J
BJ
D001482:Base Composition; D001483:Base Sequence; D002678:Chimera; D002872:Chromosome Deletion; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics*; D012320:RNA Polymerase III / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D012313:RNA; D012320:RNA Polymerase III