Putative new expression of genes in ixodid tick salivary gland development during feeding.
Poly(A+) mRNA-enriched fractions from salivary glands of partially fed Amblyomma americanum female ticks were translated in vitro with a rabbit reticulocyte translation system. Translated proteins were labeled with [35S]methionine, separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and identified by autoradiography. Thirty major identifiable polypeptides with molecular weights ranging from 14 to 136 kDa were synthesized from mRNA isolated from salivary glands of ticks weighing less than 100 mg. Polypeptides that comigrated at the same molecular weight were translated by mRNA from ticks at a more advanced stage of feeding (more than 300 mg) as were 8 others with molecular weights of 31, 71, 91, 106, 113, 118, and 128 kDa. Results demonstrated that differential gene expression may be stimulated in the developing salivary glands as the tick feeds.
Oaks
J F
JF
Department of Zoology, Oklahoma State University, Stillwater 74078-0464.
McSwain
J L
JL
Bantle
J A
JA
Essenberg
R C
RC
Sauer
J R
JR
D000818:Animals; D001096:Arachnid Vectors / Q000235:genetics* / Q000502:physiology; D001345:Autoradiography; D004435:Eating / Q000502:physiology; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015870:Gene Expression; D011061:Poly A / Q000032:analysis / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification*; D012469:Salivary Glands / Q000254:growth & development; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis* / Q000235:genetics; D013987:Ticks / Q000235:genetics* / Q000502:physiology
D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides; D011061:Poly A; D012313:RNA