Immunoaffinity purification of human alpha-fetoprotein (AFP) using monoclonal antibodies.
A two-step immunoaffinity isolation procedure for human alpha-fetoprotein (AFP) was developed resulting in highly purified AFP at high yield. A monoclonal antibody immunoadsorbent was used in the first step. Elution of AFP was carried out at alkaline pH by a solution of 0.5 mol/l ammonia containing 0.5 mol/l sodium chloride. To remove impurities caused by the first step, an anti-mouse immunoglobulin antibody immunoadsorbent was applied in the second step.
Kaiser
G
G
Department of Experimental and Clinical Immunology, Central Institute of Molecular Biology, Berlin-Buch, Germany.
Micheel
B
B
D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D006801:Humans; D007163:Immunosorbent Techniques; D011863:Radioimmunoassay; D000509:alpha-Fetoproteins / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000509:alpha-Fetoproteins