[Diagnosis of Chlamydia trachomatis by culture and Macchiavella's staining technique].
The author modified and tested staining according to Macchiavelli for detection of C. trachomatis in tissue cultures. As compared with IF staining by the monoclonal antibody the author found a high sensitivity (95.7%) and specificity (99.0%) of this method. Giemsa staining evaluated in passing light has a lower sensitivity (85.5%) and lower specificity (94.1%). The advantage of Macchiavelli staining is also the low price of the chemicals used. Imunofluorescent staining with the monoclonal antibody remains also now the reference method and is most reliable. For some materials which are obtained with difficulty (e.g. punctates etc.) the author recommends to use the IF technique.
Zampachová
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Mikrobiologický odbor KHS, Ceské Budĕjovice.
D001431:Bacteriological Techniques; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000254:growth & development; D005455:Fluorescent Antibody Technique; D006801:Humans; D013194:Staining and Labeling
Diagnostika Chlamydia trachomatis metodou kultivace a barvením dle Macchiavella.