Identification of the motility-related protein (MRP-1), recognized by monoclonal antibody M31-15, which inhibits cell motility.
A murine monoclonal antibody (M31-15) was identified using the penetration-inhibiting assay of a human lung adenocarcinoma cell line (MAC10) and remarkably inhibited the phagokinetic tract motility of various cancer cell lines. The antigen, motility-related protein (MRP-1), recognized by M31-15, was 25- and 28-kD proteins, and M31-15 was used to isolate a cDNA clone from a human breast carcinoma cDNA library. Sequence analysis revealed that MRP-1 had strong similarity with a B cell surface antigen (CD37), a melanoma-associated antigen (ME491), the target of an antiproliferative antibody (TAPA-1), a human tumor-associated antigen (CO-029), and the Sm23 antigen of the trematode parasite Schistosoma mansoni.
Miyake
M
M
Department of Thoracic Surgery, Kitano Hospital, Tazuke Kofukai Medical Research Institute, Osaka, Japan.
Koyama
M
M
Seno
M
M
Ikeyama
S
S
D000230:Adenocarcinoma / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD; D000954:Antigens, Surface / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D002465:Cell Movement; D004247:DNA / Q000302:isolation & purification; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis*; D060245:Tetraspanin 29; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; C554869:CD9 protein, human; C554870:Cd9 protein, mouse; D000939:Epitopes; D008562:Membrane Glycoproteins; D011506:Proteins; D060245:Tetraspanin 29; D004247:DNA