[The use of RNAse A-colloidal gold complexes for research on RNA-containing cell structures: the effect of different factors on the specificity of the labelling].
Effects of various factors on the specificity and intensity of labelling of RNA-containing structures on the ultrathin sections by RNase A--colloidal gold complexes were studied. The data obtained show that at the optimal choice and standard conditions of labelling the reproducibility of the results is achieved up to 10-20%. It makes it possible to use RNase A-gold method for the quantitative analysis of RNA distribution in the cells at various stages of cell cycle.
Popenko
V I
VI
Cherni
N E
NE
D000818:Animals; D002453:Cell Cycle; D016798:Ciliophora / Q000648:ultrastructure; D003102:Colloids; D005069:Evaluation Studies as Topic; D005347:Fibroblasts / Q000648:ultrastructure; D006046:Gold; D017979:Gold Colloid; D051379:Mice; D008854:Microscopy, Electron; D010179:Pancreas / Q000648:ultrastructure; D012313:RNA / Q000648:ultrastructure*; D051381:Rats; D012259:Ribonuclease, Pancreatic; D013194:Staining and Labeling / Q000379:methods*
D003102:Colloids; D017979:Gold Colloid; C058457:Aurodye; D012313:RNA; D006046:Gold; D012259:Ribonuclease, Pancreatic
Primenenie kompleksov RNKaza A--kolloidnoe zoloto dlia issledovaniĭ RNK-soderzhashchikh struktur kletki: vliianie razlichnykh faktorov na spetsifichnost' markirovaniia.