title: Ionic requirements for catecholamine aggregating actions on the teleost Poecilia reticulata melanophores.
abstract: Norepinephrine (NE) and phenylephrine (Phe) were employed to study the ionic requirements for alpha adrenoceptor activation in the teleost Poecilia reticulata melanophores. As expected the beta adrenoceptor blocker, propranolol, increased the sensitivity of the preparation to NE (5.8 times), and was therefore employed in all the experimental procedures. Neither cocaine (a neuronal uptake blocker) nor dexamethasone (an extraneuronal uptake blocker) enhanced the sensitivity of the preparation to NE, suggesting that these inactivating mechanisms would not play a role in P. reticulata pigmentary system. However, in the absence of calcium, the dose-response curve (DRC) to NE was displaced to the left about 3.5 times, whereas the DRC to Phe was not affected. These results indicate that a neuronal uptake is active, but was not demonstrated by the classical pharmacological tools, probably due to an assymmetric display of the nervous endings. The DRC to NE was rightward displaced (14.1 times) in the presence of the calcium channel blocker Verapamil, whereas the DRC to Phe was not affected. These data suggest that P. reticulata melanophores possess a mixed population of alpha 1 and alpha 2 adrenoceptors, the activation of the latter eliciting an extracellular calcium influx. In sodium-free saline, the DRC to NE was rightward shifted (6.6 times) and the response to Phe was impaired in such a way that the maximal response was not achieved. The DRC to both NE and Phe were rightward displaced (7.9 and 2.7 times respectively) in the presence of the sodium channel blocker tetrodotoxin (TTX) 10(-7)M.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Departamento de Fisiologia Geral, Universidade de São Paulo, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Visconti
authors.forename: A M
authors.initials: AM
authors.lastname: Castrucci
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D003042:Cocaine / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology; D007477:Ions; D008547:Melanophores / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009638:Norepinephrine / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D011036:Poecilia / Q000502:physiology*; D011433:Propranolol / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
chemical_list: D002395:Catecholamines; D007473:Ion Channels; D007477:Ions; D011942:Receptors, Adrenergic, alpha; D010656:Phenylephrine; D013779:Tetrodotoxin; D003907:Dexamethasone; D011433:Propranolol; D014700:Verapamil; D003042:Cocaine; D009638:Norepinephrine