title: The major outer membrane protein of Acidovorax delafieldii is an anion-selective porin.
abstract: The major outer membrane protein (Omp34) of Acidovorax delafieldii (formerly Pseudomonas delafieldii) was purified to homogeneity and was characterized biochemically and functionally. The polypeptide has an apparent molecular weight (Mr) of 34,000, and it forms stable oligomers at pH 9.0 in the presence of 10% octylpolyoxyethylene or 2% lithium dodecyl sulfate below 70 degrees C. The intact protein has a characteristic secondary structure composition, as revealed by Fourier transforming infrared spectroscopy (about 60% beta sheet). These features and the amino acid composition are typical for porins. The purified Omp34 is associated with 1 to 2 mol of lipopolysaccharide per mol of the monomer. Pore-forming activity was demonstrated with lipid bilayer experiments. Single-channel and selectivity measurements showed that the protein forms highly anion-selective channels. The unusual dependence of the single-channel conductance on salt concentration suggests that the porin complexes bear positive surface charges, accumulating negatively charged counterions at the pore mouth.
authors.affiliation: Abteilung für Molekulare Strukturbiologie, Max-Planck Institut für Biochemie, Martinsried, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Brunen
authors.forename: H
authors.initials: H
authors.lastname: Engelhardt
authors.forename: A
authors.initials: A
authors.lastname: Schmid
authors.forename: R
authors.initials: R
authors.lastname: Benz
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification*; D007473:Ion Channels / Q000378:metabolism*; D008051:Lipid Bilayers; D008564:Membrane Potentials; D008970:Molecular Weight; D018272:Porins; D011487:Protein Conformation; D011549:Pseudomonas / Q000032:analysis*; D012492:Salts / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; D018272:Porins; D012492:Salts