Essential role of cholecystokinin in pancreatic regeneration after 60% distal resection in rats.
The essential role of cholecystokinin (CCK) in pancreatic regeneration after pancreatitis or resection has been supposed, but not yet clearly demonstrated. In rats, 6-8 weeks after 60% distal resection of the pancreas a gradual increase in pancreatic weight and contents of DNA, protein, trypsin, chymotrypsin and amylase, occurred (there was no increase in lipase); Pancreatic regeneration stopped thereafter. Nonparallel increases in enzyme values were similar to those seen after CCK administration. Indeed, basal CCK levels increased significantly by the 6th week and declined thereafter. A one month s.c. administration of CCK-octapeptide (CCK-8) (3 x 300 ng/kg/d) accelerated regeneration in the first month, but it had almost no effect during the second or third postoperative months. A two week s.c. administration of a specific CCK antagonist, CR 1409 (3 x 4 mg/kg/d) totally prevented regeneration by the fifth and sixth weeks, but did not diminish pancreatic weight or DNA and protein contents during the first two weeks. Alcohol administration (12 g/kg/d) reduced CCK release and prevented pancreatic regeneration during the three-month experimental period. These data indicate that CCK has an essential role in pancreatic regeneration and that the deleterious effect of alcohol on regeneration involves inhibition of CCK release.
Pap
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First Department of Medicine, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Boros
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L
Hajnal
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F
D000681:Amylases / Q000378:metabolism; D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism / Q000502:physiology*; D002918:Chymotrypsin / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000378:metabolism / Q000502:physiology* / Q000601:surgery; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D014357:Trypsin / Q000378:metabolism
D011506:Proteins; D004247:DNA; D002766:Cholecystokinin; D000681:Amylases; D002918:Chymotrypsin; D014357:Trypsin