A DNA sequence upstream of the tet(O) gene is required for full expression of tetracycline resistance.
The DNA sequences upstream of the tet(O) and tet(M) open reading frames (ORFs) (ca. 300 bp) were found to share a higher degree of homology than those of the tet(O) and tet(M) ORFs themselves. A transcription initiation site for tet(O) was located by primer extension analysis. Campylobacter coli was found to use a promoter sequence different from that used by Escherichia coli. The sequence upstream of tet(O) was shown to be required in cis for high-level resistance to tetracycline.
Wang
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Department of Microbiology, University of Alberta, Edmonton, Canada.
Taylor
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D001483:Base Sequence; D017000:Campylobacter coli / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames; D012329:RNA, Bacterial / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013753:Tetracycline Resistance / Q000235:genetics*; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012329:RNA, Bacterial