Molecular cloning and sequence analysis of the cDNA encoding the human acrosin-trypsin inhibitor (HUSI-II).
A complete cDNA clone encoding the human acrosin-trypsin inhibitor HUSI-II has been isolated from a cDNA library of human testis and completely sequenced. The cDNA of 594 bp contained an open reading frame of 252 base pairs. The deduced amino acid sequence comprised the complete amino acid sequence of HUSI-II and a putative signal peptide. Northern blotting analysis revealed that HUSI-II is synthesized in testis, epididymis and seminal vesicle, but not in the prostate gland.
Möritz
A
A
Department of Clinical Chemistry and Clinical Biochemistry, University of Munich, Germany.
Lilja
H
H
Fink
E
E
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D006023:Glycoproteins; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D000074162:Serine Peptidase Inhibitors, Kazal Type; D013737:Testis / Q000378:metabolism; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000235:genetics*
D006023:Glycoproteins; C066238:SPINK2 protein, human; D000074162:Serine Peptidase Inhibitors, Kazal Type; D014359:Trypsin Inhibitor, Kazal Pancreatic; D012313:RNA; D004247:DNA