Potentiation of bleomycin cytotoxicity by high molecular weight polyacrylic acid. II. Involvement of rapid conformational change of polyacrylate.
We report that bleomycin (BLM) cytotoxicity is dramatically potentiated in the presence of high molecular weight polyacrylic acid (A119). For potentiation, an appropriate amount of divalent cations such as Ca++, Mg++, or Ba++ was required, in addition to vortex - stirring of the reaction mixture. There was no potentiation when A119 alone was pre-stirred or left standing for several days in the presence of divalent cations prior to use. Taking into account of kinematic viscosity of A119 observed during the treatment, a rapid conformational change of A119 in the presence of divalent cations might be involved in the potentiation mechanism.
Suzuki
T
T
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Sarai
K
K
Kohda
K
K
Kawazoe
Y
Y
D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D004357:Drug Synergism; D007700:Kinetics; D007939:Leukemia L1210; D051379:Mice; D008968:Molecular Conformation; D014783:Viscosity
D000180:Acrylic Resins; D000970:Antineoplastic Agents; D002413:Cations, Divalent; D001761:Bleomycin; C006903:carbopol 940; D002118:Calcium