Epitope mapping of the low-molecular-mass subunits of reverse transcriptase in human immunodeficiency virus type 1 by monoclonal antibodies.
With the aid of monoclonal antibodies to the reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1), low-molecular-mass subunits (p29, p32, and p40) were identified in HIV-1 RT purified from HIV (HTLV-IIIB) virions by isoelectric focusing. Epitope mapping with synthetic polypeptides from various regions of the pol gene suggests that the low-molecular-mass subunits result from N-terminal cleavage of the p51 subunit. The subunits could be separated only by SDS-polyacrylamide gel electrophoresis and detected by immunoblotting. They could not be separated on chromatographic columns, suggesting that the subunits are complexed or conformationally arranged in such a way that their separation on the basis of molecular mass is not possible. The molecular mass of the active enzyme eluted from a chromatographic column (Sephacryl S-300) loaded with a mixture of the subunits was estimated to be 100 kDa.
Chandra
P
P
Laboratory of Molecular Biology, University Medical School, Frankfurt am Main, FRG.
Gerber
T
T
Chandra
A
A
Sarin
P S
PS
Pavlikov
S P
SP
Sidorovich
I
I
Khaitov
R
R
Ivanov
V
V
Koziach
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D000939:Epitopes / Q000737:chemistry; D015751:Genes, pol; D015488:HIV Antigens / Q000737:chemistry*; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D007120:Immunochemistry; D051379:Mice; D008970:Molecular Weight; D010449:Peptide Mapping; D011487:Protein Conformation; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015488:HIV Antigens; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase