The identification of the bacteriophage HP1c1 and S2 integration sites in Haemophilus influenzae Rd by field-inversion gel electrophoresis of large DNA fragments.
The resolution of high molecular weight DNA fragments by field-inversion gel electrophoresis (FIGE) demonstrate the presence of two phage (S2 and HP1c1) integration sites (attB) in the Haemophilus influenzae Rd chromosome. In a population of wild-type cells either prophage site appears to be occupied in a single cell by one to at least three, tandemly repeated, amplified phage DNA molecules. The attL of the second bacterial attachment site present in the host SmaI fragment 7 and the leftmost part of phage S2 type B DNA of its genome organization (Piekarowicz et. al., 1986) have been sequenced. A comparison of the two bacterial att sites demonstrated that their homology is limited to the core region. A comparison of the DNA sequences of phage S2 type B and HP1c1 type C revealed a 530-bp insertion in the HP1c1 type C (not present in S2 type B) in addition to DNA variants due mostly to single-base mismatches. We postulate that phage S2 and HP1c1 genome variants (A, B, and C) evolved from a single phage origin and might stem from passage history arisen through accumulation of mutations.
Kauc
L
L
Institute of Microbiology, Warsaw University, Poland.
Skowronek
K
K
Goodgal
S H
SH
D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000737:chemistry; D004586:Electrophoresis; D006193:Haemophilus influenzae / Q000235:genetics*; D008242:Lysogeny; D008969:Molecular Sequence Data
D004279:DNA, Viral