Estrogen-dependent alterations in differentiation state of myeloid cells caused by a v-myb/estrogen receptor fusion protein.
The oncogene v-myb and its cellular progenitor c-myb encode nuclear, DNA binding phosphoproteins that are thought to regulate the expression of myb-responsive genes during myeloid differentiation. To identify such myb-regulated genes, and to explore the mechanisms by which v-myb affects their expression, we have established a conditional expression system for v-myb. We have converted the v-myb protein to an estrogen-inducible transactivator by fusing the protein to the hormone binding domain of the human estrogen receptor. Expression of the chimeric protein in a chicken macrophage cell-line causes estrogen-dependent, reversible changes in the differentiation state as well as alterations in the gene expression program of the cells. We have used this estrogen-dependent v-myb expression system to identify a novel v-myb regulated gene.
Burk
O
O
Hans-Spemann-Laboratory, Max-Planck-Institute for Immunobiology, Freiburg, FRG.
Klempnauer
K H
KH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D002645:Chickens; D003370:Coturnix; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D020626:Oncogene Proteins v-myb; D012313:RNA / Q000032:analysis; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators
D004268:DNA-Binding Proteins; D004967:Estrogens; D020626:Oncogene Proteins v-myb; D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins; D015682:Retroviridae Proteins, Oncogenic; D015534:Trans-Activators; D012313:RNA; D004247:DNA