title: A simple assay for proteolysis of IGFBP-3.
abstract: An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125IhIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37 degrees in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associated protease is Ca++ dependent. The substrate is also proteolyzed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.
authors.affiliation: Department of Pediatrics, Stanford University Medical Center, California 94305.
authors.forename: G
authors.initials: G
authors.lastname: Lamson
authors.forename: L C
authors.initials: LC
authors.lastname: Giudice
authors.forename: R G
authors.initials: RG
authors.lastname: Rosenfeld
mesh_terms: D000818:Animals; D001345:Autoradiography; D002352:Carrier Proteins / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D018969:Insulin-Like Growth Factor Binding Proteins; D051379:Mice; D010447:Peptide Hydrolases / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D011994:Recombinant Proteins; D013002:Somatomedins / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D011994:Recombinant Proteins; D013002:Somatomedins; D010447:Peptide Hydrolases