title: The chloroplast gene for ribosomal protein CL23 is functional in tobacco.
abstract: Chloroplast rpl23 loci potentially coding for a polypeptide homologous to the E. coli L23 ribosomal protein are frame-shifted in spinach and several other plants, indicating that these loci are pseudogenes. In tobacco, rpl23 constitutes a continuous open reading frame of 93 codons and its transcript initiates at least 66 bp upstream from the initiation codon. The N-terminal amino acid sequence of a 13 kDa protein from the 50 S subunit of tobacco chloroplast ribosomes matches that derived from the tobacco rpl23 locus. This shows that rpl23 is a functional gene in tobacco.
authors.affiliation: Center for Gene Research, Nagoya University, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Yokoi
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Wakasugi
authors.forename: M
authors.initials: M
authors.lastname: Sugiura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000502:physiology*; D008969:Molecular Sequence Data; D010940:Plant Proteins; D010947:Plants, Toxic; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D010940:Plant Proteins; D012269:Ribosomal Proteins; C068384:Rpl23 protein, Nicotiana tabacum; D012313:RNA