Pituitary adenylate cyclase activating polypeptide stimulates insulin release from the isolated perfused rat pancreas.
Pituitary adenylate cyclase activating polypeptide (PACAP) is a novel hypothalamic peptide structurally related to vasoactive intestinal peptide (VIP) and glucagon like peptide-1(7-36) amide (tGLP-1) in its N-terminal portion. Therefore, their levels of insulinotropic potency were compared using an isolated rat pancreas perfusion. It was found that 0.1 nM PACAP (1-27) amide (PACAP27) significantly stimulated insulin release under a perfusate glucose concentration of 5.5 mM, whereas 1 nM PACAP27 did not under a perfusate glucose concentration of 2.8 mM. The potency was evaluated as tGLP-1 greater than PACAP27 greater than VIP. These results indicate that PACAP is a glucagon superfamily peptide which stimulates insulin release in a glucose dependent manner.
Kawai
K
K
Department of Internal Medicine, University of Tsukuba, Ibaraki-ken, Japan.
Ohse
C
C
Watanabe
Y
Y
Suzuki
S
S
Yamashita
K
K
Ohashi
S
S
D000595:Amino Acid Sequence; D000818:Animals; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000494:pharmacology*; D010477:Perfusion; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051381:Rats
C495403:Adcyap1 protein, rat; D007328:Insulin; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide