title: Conservation of the HLA-DQB2 locus in nonhuman primates.
abstract: The evolutionary history of MHC class II genes is characterized by several examples of gene duplication, leading both to the creation of distinct subregions such as DR, DQ, and DP, as well as to duplicated loci within each of these subregions. In the human MHC, a prominent example of this diversification occurs within the HLA-DQ subregion, where the nonpolymorphic and transcriptionally "silent" DQB2 locus is highly homologous to the polymorphic expressed DQB1 locus. In order to gain some insight into the mechanisms constraining polymorphism at the DQB2 locus, the second exons of five nonhuman primate DQB2 alleles were sequenced. Six nonhuman primate DQB2 analogous sequences were obtained, two each from chimpanzee and owl monkey cell lines, and one each from gorilla and gibbon cell lines. Notably, the DQB2 sequences from the gibbon, gorilla, and one of the two chimpanzee sequences, although containing some silent nucleotide changes, encode a predicted DQB2 protein with 100% homology to the human DQB2 sequence. The owl monkey DQB2 allelic sequences and the other chimpanzee DQB2 sequence contain additional polymorphisms, but maintain approximately 95% nucleotide sequence identity with human and the other primate sequences. Identification of the owl monkey DQB2 locus indicates that the ancestral DQ gene duplication event occurred at least 40 million years ago, rather than 10 million years, as previously thought. Remarkably, nucleotide sequences from amplified cDNA indicate that the DQB2 gene, and not the DQB1 gene, may be transcribed in the owl monkey line. Substitutions occur at sites comparable to codons of well-recognized allelic variation in the functional DQB1 genes, implying that variation within the DQB2 locus operates under similar selection constraints to the DQB1 locus, with an extremely high degree of conservation through primate evolution.
authors.affiliation: Department of Microbiology and Immunology, Bowman Gray School of Medicine, Winston-Salem, NC 27103.
authors.forename: L K
authors.initials: LK
authors.lastname: Gaur
authors.forename: E R
authors.initials: ER
authors.lastname: Heise
authors.forename: P S
authors.initials: PS
authors.lastname: Thurtle
authors.forename: G T
authors.initials: GT
authors.lastname: Nepom
mesh_terms: D000818:Animals; D001029:Aotus trivirgatus / Q000235:genetics; D001483:Base Sequence; D015870:Gene Expression; D006071:Gorilla gorilla / Q000235:genetics; D006681:HLA-D Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D006801:Humans; D006922:Hylobates / Q000235:genetics; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000235:genetics; D011323:Primates / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D006681:HLA-D Antigens; D006683:HLA-DQ Antigens; C040626:HLA-Dx antigen; D012333:RNA, Messenger