[The detection and nature of the labelling of F-actin using a phalloidin-colloidal gold marker].
On the basis of a conjugate of phalloidin-colloidal gold a marker has been obtained for electronmicroscopic identification and study of marking character of filament actins. Using this marker the filamentous form of actins from rabbit skeletal muscles and chicken gizzard was revealed. The marking characters of the two actins appeared to differ at the level of electron microscopy. A periodicity of the marker setting with a step of 105.4 +/- 1.7 nm on the rabbit F-actin was revealed. A possibility of differential evaluation of the filamentous and globular forms of the actins was shown when using the same marker in dot-blot analysis procedure with the sensitivity from 15 to 50 ng.
Rikhter
T Ia
TIa
Gringauz
O K
OK
Sokolov
O I
OI
D000199:Actins / Q000032:analysis / Q000648:ultrastructure*; D000818:Animals; D002645:Chickens; D003102:Colloids; D006046:Gold; D015151:Immunoblotting; D007150:Immunohistochemistry / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods; D009130:Muscle, Smooth / Q000737:chemistry / Q000648:ultrastructure; D010590:Phalloidine; D011817:Rabbits; D013194:Staining and Labeling / Q000379:methods*
D000199:Actins; D003102:Colloids; D010590:Phalloidine; D006046:Gold
Vyiavlenie i kharakter mecheniia F-aktina markerom falloidin--kolloidnoe zoloto.