[The effect of a new immunosuppressive agent, FK-506, on xenogeneic neural transplantation in rodents--comparison with cyclosporin A].
The present study was performed to compare the immunosuppressive effect of FK-506 (FK) with cyclosporin A (CyA) in xenogeneic neural transplantation in rodents. The solid grafts of embryonic ventral mesencephalic tissue from 14-day rat embryos were transplanted into the right lateral ventricle of 20 mature male mice using a stereotactic approach, and the mice were sacrificed 14 days after transplantation. The removed brains, which were sectioned coronally, were stained with antibodies against glial fibrillary acidic protein (GFAP), Lyt-2 or tyrosine hydroxylase (TH) in addition to hematoxylin-eosin and Nissl stainings. The subcutaneous (s.c.) administration of FK at a dose of 10.0 mg/kg daily for 14 days enabled the neural xenografts to survive and grow (n = 5), although all of the neural xenografts treated with CyA at a dose of 10.0 mg/kg s.c. (n = 5) or FK at a dose of 1.0 mg/kg s.c. (n = 5) daily for 14 days and ones treated without such an immunosuppressive agent (n = 5) were rejected by immunological reactions. We conclude that FK is a more powerful immunosuppressive agent than CyA in xenogeneic neural transplantation as well as in cardiac and renal allograftings.
Sakai
K
K
Department of Neurological Surgery, Okayama University Medical School, Japan.
Date
I
I
Yoshimoto
Y
Y
Arisawa
T
T
Nakashima
H
H
Furuta
T
T
Asari
S
S
Nishimoto
A
A
D000818:Animals; D016380:Brain Tissue Transplantation / Q000276:immunology*; D016572:Cyclosporine / Q000494:pharmacology; D006084:Graft Rejection / Q000187:drug effects*; D006085:Graft Survival / Q000187:drug effects; D008297:Male; D008636:Mesencephalon / Q000637:transplantation*; D051379:Mice; D008809:Mice, Inbred C3H; D051381:Rats; D011919:Rats, Inbred Strains; D016559:Tacrolimus / Q000494:pharmacology*; D014183:Transplantation, Heterologous / Q000276:immunology*
D016572:Cyclosporine; D016559:Tacrolimus