The sgp-60 molecule is linked to the plasma membrane via a glycosylphosphatidylinositol anchor.
We have recently identified a novel murine glycoprotein termed sgp-60, which is expressed on the cell surface of T and B lymphocytes. Because of the profound modulatory effects of sgp-60 on activation through the T cell receptor/CD3 complex, we have examined the membrane attachment domain of the molecule. sgp-60 is not expressed on the surface of variants of a T-T hybridoma cell line that are defective in glycosylphosphatidylinositol (GPI) anchor biosynthesis. In wild-type but not in mutant cells, sgp-60 can be labeled with palmitic acid. Furthermore, the molecule can be removed from the cell surface of both T and B lymphocytes by enzymatic digestion with a phosphatidylinositol-specific phospholipase C. We conclude that the sgp-60 molecule is linked to the plasma membrane via a GPI anchor.
González Cabrero
J
J
Department of Pathology, Harvard Medical School, Boston, MA.
Reiser
H
H
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000737:chemistry; D018968:CD58 Antigens; D002462:Cell Membrane / Q000737:chemistry; D006017:Glycolipids / Q000502:physiology*; D006023:Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D010716:Phosphatidylinositols / Q000502:physiology*; D013601:T-Lymphocytes / Q000737:chemistry; D010738:Type C Phospholipases / Q000494:pharmacology
D000954:Antigens, Surface; D018968:CD58 Antigens; D006017:Glycolipids; D006023:Glycoproteins; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D010716:Phosphatidylinositols; D010738:Type C Phospholipases