[The differentiation of viruses of the tick-borne encephalitis complex by means of RNA-DNA hybridization].
Nucleic acid spot hybridization with cloned cDNA of tick-borne encephalitis (TBE) virus, strain Sofjin, was used to differentiate strains of TBE and other flaviviruses. The cDNA probe reacted with strains of TBE and flaviviruses of TBE subgroup with the exception of Powassan virus. The probe did not react with viruses of Japanese encephalitis and Gendue subgroups. The viruses of TBE subgroup and some strains of TBE virus were differentiated from TBE strain Sofjin by thermal stability of RNA-DNA hybrids. Negishi and Louping ill viruses were found to be most closely related to TBE strain Sofjin among viruses of the TBE subgroup.
Shamanin
V A
VA
Pletnev
A G
AG
Rubin
S G
SG
Zlobin
V I
VI
D000818:Animals; D001921:Brain; D004247:DNA / Q000235:genetics; D015342:DNA Probes / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics*; D004669:Encephalitis Viruses, Tick-Borne / Q000145:classification* / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D005821:Genetic Techniques; D051379:Mice; D009693:Nucleic Acid Hybridization / Q000235:genetics*; D012313:RNA / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*
D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral; D012313:RNA; D004247:DNA
Differentsiatsiia virusov kompleksa kleshchevogo éntsefalita metodom RNK--DNK-gibridizatsii.