title: A DNA sequence upstream of the tet(O) gene is required for full expression of tetracycline resistance.
abstract: The DNA sequences upstream of the tet(O) and tet(M) open reading frames (ORFs) (ca. 300 bp) were found to share a higher degree of homology than those of the tet(O) and tet(M) ORFs themselves. A transcription initiation site for tet(O) was located by primer extension analysis. Campylobacter coli was found to use a promoter sequence different from that used by Escherichia coli. The sequence upstream of tet(O) was shown to be required in cis for high-level resistance to tetracycline.
authors.affiliation: Department of Microbiology, University of Alberta, Edmonton, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: D E
authors.initials: DE
authors.lastname: Taylor
mesh_terms: D001483:Base Sequence; D017000:Campylobacter coli / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames; D012329:RNA, Bacterial / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013753:Tetracycline Resistance / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial