title: Polymorphism of the CD4 and CD5 differentiation antigens in cattle.
abstract: Monoclonal antibodies (mAbs) specific for bovine CD4 and CD5 antigens have been found to identify polymorphic determinants on these molecules. In the case of CD5, mAb IL-A67 recognises one allotypic form of the antigen while four other CD5-specific mAbs in the workshop (CC17, CC29, BLT-1 and 8C11) recognise a second allotype. The CD4-specific mAbs submitted to the workshop reacted with the cells of all animals tested. However, a further two mAbs (CC26 and IL-A18) specific for CD4 were found to react with cells only from about 85% of animals tested. Sequential immuno-precipitation experiments together with family studies showed that the allotypes of CD4 and CD5 are both inherited in a simple Mendelian manner and are co-dominantly expressed. One of the CD5 allotypes was not detected in Bos taurus animals while the gene frequency of the second allotype was only about 10% in the B. indicus animals tested. The gene frequency of the CD4 allotype detected by CC26 and IL-A18 was similar in the two sub-species.
authors.affiliation: ILRAD, Nairobi, Kenya.
authors.forename: W I
authors.initials: WI
authors.lastname: Morrison
authors.forename: C J
authors.initials: CJ
authors.lastname: Howard
authors.forename: C A
authors.initials: CA
authors.lastname: Hinson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000235:genetics* / Q000276:immunology; D018956:CD5 Antigens; D002417:Cattle / Q000276:immunology*; D005434:Flow Cytometry; D007519:Isoantigens / Q000235:genetics / Q000276:immunology; D011110:Polymorphism, Genetic; D011233:Precipitin Tests
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018956:CD5 Antigens; D007519:Isoantigens