Short Nissl staining for incubated cryostat sections of the brain.
Nissl stain often binds poorly to cryostat sections which have been incubated in solutions of radiolabeled ligands. Such incubation is used in receptor autoradiography of the brain when using the in vitro method. We have developed a rapid (16 min) modification of Nissl staining for sections that bind stain poorly, e.g., incubated sections. The method stains well sections which cannot be stained with other rapid Nissl staining methods.
Lindroos
O F
OF
Department of Physiology, University of Helsinki, Finland.
D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000648:ultrastructure; D002951:Citrates; D019343:Citric Acid; D005629:Frozen Sections; D008751:Methylene Blue; D009562:Nissl Bodies / Q000648:ultrastructure*; D051381:Rats; D013194:Staining and Labeling; D014048:Tolonium Chloride
D002951:Citrates; D014048:Tolonium Chloride; D019343:Citric Acid; D008751:Methylene Blue