title: Fractionated irradiation and early changes in salivary glands. Different effects on potassium efflux, exocytotic amylase release and gland morphology.
abstract: Irradiation is a potent treatment modality of head and neck cancer. However, the irradiation is usually associated with an influence on salivary glands with ensuing dryness and discomfort for the patients. In the present study we used different in vitro secretory models and morphologic characterization of rat parotid gland. Radiation was given to one gland on a 5-day schedule with 6 MV photons (total dose 20, 30, 35, 40, 45 Gy). The contralateral gland served as control, and the analysis of glands were performed 10 days after the last irradiation treatment. The noradrenaline stimulated electrolyte secretion (86rubidium tracer for potassium) was decreased in relation to the irradiation dose and in comparison to contralateral control glands. Noradrenaline stimulated exocytotic amylase release was not affected by irradiation and, there were no signs of obvious quantitative morphologic alterations after irradiation compared with controls. The results suggest that there are differences in the sensitivity to radiation for the two different secretory processes in salivary glands, and, thus, the structures regulating electrolyte and fluid secretion seem to be more vulnerable to irradiation than the process of exocytosis. The results, however, do not allow discrimination between temporary cellular impairment and irreversible damage leading to cell death.
authors.affiliation: Department of Oncology, University Hospital, Umeå, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Franzén
authors.forename: U
authors.initials: U
authors.lastname: Funegård
authors.forename: S
authors.initials: S
authors.lastname: Sundström
authors.forename: H
authors.initials: H
authors.lastname: Gustafsson
authors.forename: A
authors.initials: A
authors.lastname: Danielsson
authors.forename: R
authors.initials: R
authors.lastname: Henriksson
mesh_terms: D000681:Amylases / Q000378:metabolism*; D000818:Animals; D005089:Exocytosis; D007700:Kinetics; D009638:Norepinephrine / Q000494:pharmacology; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D010315:Particle Accelerators; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012413:Rubidium / Q000378:metabolism; D012414:Rubidium Radioisotopes
chemical_list: D012414:Rubidium Radioisotopes; D000681:Amylases; D012413:Rubidium; D011188:Potassium; D009638:Norepinephrine