title: Dexamethasone-induced catabolism and insulin resistance in L6 myoblasts are reversed by the removal of serum.
abstract: 1. One hundred nanomolar dexamethasone reduced protein synthesis by 16% and also decreased the accretion of protein and RNA in L6 myoblasts when foetal calf serum was present; these effects were reversed when serum was omitted from the medium. 2. Insulin (100 microU/ml) increased protein synthesis, protein accretion and RNA accretion both in the presence and the absence of serum. 3. Dexamethasone inhibited the effects of 100 microU insulin/ml in the presence of serum and induced insulin resistance; in the presence of 25 or 100 nM dexamethasone insulin was ineffective at concentrations below 250 microU and 1 mU/ml respectively.
authors.affiliation: Rowett Research Institute, Bucksburn, Aberdeen, Scotland.
authors.forename: R M
authors.initials: RM
authors.lastname: Palmer
authors.forename: P A
authors.initials: PA
authors.lastname: Bain
authors.forename: B G
authors.initials: BG
authors.lastname: Southorn
mesh_terms: D000818:Animals; D001769:Blood; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D004347:Drug Interactions; D007328:Insulin / Q000494:pharmacology; D007333:Insulin Resistance; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism
chemical_list: D007328:Insulin; D011506:Proteins; D012313:RNA; D003907:Dexamethasone