Glycol methacrylate embedding in diagnostic pathology. A standardized method for processing and embedding human tissue biopsy specimens.
A standardized "low-cost" processing and embedding procedure is described for the preparation of semithin glycol methacrylate (GMA) sections of human tissue biopsy specimens for use in routine diagnostic pathology. The method is highly reliable and reproducible and is based on many years of experience in fundamental GMA research. The procedure combines the application of new, less toxic, plasticizers and the preparation of high-quality GMA mixtures. Microwave irradiation was used to improve staining qualities and to reduce staining times. An improved reticulin staining procedure is presented as well.
Gerrits
P O
PO
Department of Anatomy and Embryology, University of Groningen, The Netherlands.
Suurmeijer
A J
AJ
D001706:Biopsy; D001853:Bone Marrow / Q000033:anatomy & histology; D006652:Histological Techniques; D006801:Humans; D008689:Methacrylates; D008867:Microtomy; D008872:Microwaves; D013194:Staining and Labeling / Q000379:methods
D008689:Methacrylates; C005044:hydroxyethyl methacrylate