Colloidal gold as a permanent marker of cells.
We have demonstrated that colloidal gold-labelled serum proteins are taken up by a number of cells in cultures established from the postnatal rodent neopallium. The colloidal gold enters and remains within secondary lysosomes over extended periods of time and, as well, persists after the subculture of these cells. The cell types that readily take up the label in our culture system are type-1 astrocytes, glial precursor cells and macrophages, whereas, only a small number of oligodendrocytes take up the label. The use of serum proteins to introduce colloidal gold into cells therefore seems to be a convenient and easy way to permanently mark cells.
Juurlink
B H
BH
Department of Anatomy, University of Saskatchewan, Saskatoon, Canada.
Devon
R M
RM
D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D003102:Colloids; D046508:Culture Techniques / Q000379:methods; D006046:Gold; D017979:Gold Colloid; D051379:Mice; D008853:Microscopy / Q000379:methods; D013194:Staining and Labeling
D003102:Colloids; D017979:Gold Colloid; C058457:Aurodye; D006046:Gold