title: Interleukin 8-inhibitor and inducer of histamine and leukotriene release in human basophils.
abstract: We have shown previously that interleukin 8 (IL-8) induces histamine and leukotriene release in human basophils exposed to interleukin 3 (IL-3). We now found that pretreatment with low concentrations of IL-8 selectively inhibits this response. Inhibition was significant at 0.01 nM IL-8 and virtually complete at 1 nM, which is about 100-fold lower than the concentration required for induction of mediator release. IL-8 dependent responses were also inhibited, albeit to a lesser extent, by preincubation with neutrophil-activating peptide 2 (NAP-2), but not with connective tissue-activating peptide III (CTAP-III) or platelet factor 4 (PF4). Release induced by C5a, fMet-Leu-Phe, or anti-IgE antibody, by contrast, was not affected.
authors.affiliation: Institute of Clinical Immunology, Inselspital, Bern, Switzerland.
authors.forename: S C
authors.initials: SC
authors.lastname: Bischoff
authors.forename: M
authors.initials: M
authors.lastname: Baggiolini
authors.forename: A L
authors.initials: AL
authors.lastname: de Weck
authors.forename: C A
authors.initials: CA
authors.lastname: Dahinden
mesh_terms: D001491:Basophils / Q000187:drug effects / Q000502:physiology*; D015936:Complement C5a / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000494:pharmacology*; D007700:Kinetics; D015289:Leukotrienes / Q000097:blood* / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D016209:Interleukin-8; D015289:Leukotrienes; D011994:Recombinant Proteins; D015936:Complement C5a