title: High performance liquid chromatography (HPLC): a simple method to quantify Hb C, O-Arab, Agenogi and F.
abstract: Differentiation of some abnormal haemoglobins, such as Hb C, O-Arab, Agenogi, E, O-Indonesia, C-Harlem, and Siriraj, is difficult and quantitation of the various fractions is impossible with cellulose acetate electrophoresis. The authors report 13 cases of Hb C, 10 of Hb O-Arab and 5 of Hb Agenogi whose haemoglobin fractions were quantitated by HPLC during a thalassaemia screening programme. Hb F was determined by both Betke's method and HPLC. Analysis of data by linear regression demonstrates that the methods furnish overlapping results. Our findings show that HPLC is a rapid and easily reproduced method which allows quantitative and qualitative discrimination of the various haemoglobin fractions, making it a valid tool in screening programmes for haemoglobinopathies.
authors.affiliation: Cattedra di Ematologia Pediatrica, Università di Catania, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Samperi
authors.forename: G R
authors.initials: GR
authors.lastname: Mancuso
authors.forename: S P
authors.initials: SP
authors.lastname: Dibenedetto
authors.forename: A
authors.initials: A
authors.lastname: Di Cataldo
authors.forename: R
authors.initials: R
authors.lastname: Ragusa
authors.forename: G
authors.initials: G
authors.lastname: Schilirò
mesh_terms: D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005319:Fetal Hemoglobin / Q000032:analysis*; D006444:Hemoglobin C / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D008403:Mass Screening; D008875:Middle Aged; D015203:Reproducibility of Results; D013789:Thalassemia / Q000175:diagnosis*
chemical_list: D006455:Hemoglobins, Abnormal; D006444:Hemoglobin C; C057245:hemoglobin Agenogi; D005319:Fetal Hemoglobin; C032031:hemoglobin Arab