The effect of in ovo boron supplementation on bone mineralization of the vitamin D-deficient chicken embryo.
It has been hypothesized that boron (B) is an essential element for animals, but its action will vary greatly depending on the nutriture of the organism. One of the nutrients implicated as having an interaction with boron is cholecalciferol (Vit D3). This study was carried out to determine if such an interaction exists. The study was conducted utilizing vitamin D-deficient chicken embryos that were injected through the shell at 8 d of embryogenesis with carrier (NaCl and/or acetone), B (0.5 mg), B + Vit D3 (0.5 mg and 0.3 microgram, respectively), or Vit D3 (0.3 or 1.5 micrograms). The in ovo concomitant administration of boron and vitamin D enhanced (p less than 0.05) the hatchability of the vitamin D-deficient embryos. Furthermore, boron and/or vitamin D3 increased (p less than 0.05) the percent of bone ash and decreased (p less than 0.05) the exaggerated height of the proliferative zone of the epiphyseal growth plate normally observed in vitamin D deficiency, suggesting a more rapid bone formation. The results provide further evidence supporting the hypothesis that boron plays a role in bone mineralization through an interaction with vitamin D.
King
N
N
United States Army Research Institute of Environmental Medicine, College Station, TX.
Odom
T W
TW
Sampson
H W
HW
Yersin
A G
AG
D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects*; D001895:Boron / Q000494:pharmacology*; D002117:Calcitriol / Q000494:pharmacology*; D002642:Chick Embryo; D002645:Chickens; D005260:Female; D010012:Osteogenesis / Q000187:drug effects*; D014808:Vitamin D Deficiency / Q000196:embryology* / Q000517:prevention & control
D002117:Calcitriol; D001895:Boron