[P-31 NMR studies of in vivo regeneration of preservative-induced changes in 2,3-diphosphoglycerate content of erythrocytes].
In this study, we analyzed the regeneration kinetics and the reversibility of the 2.3-DPG reduction in stored red cells after transfusion. Within 3 h after transfusion the 2.3-DPG levels raised up to 40% of the patients' prior 2.3-DPG concentration, although the 2.3-DPG content of the transfused red cells was less than 10% of before. The complete regeneration of the 2.3-DPG concentration occurred after 36 to 48 h after transfusion. There was a close correlation of the results obtained by P-31-NMR and enzymatic determination of DPG.
Matthes
G
G
Institut für Transfusiologie und Transplantologie des Bereiches Medizin (Charite), Humboldt-Universität zu Berlin.
Strunk
S
S
Storek
W
W
Strauss
D
D
D019794:2,3-Diphosphoglycerate; D001793:Blood Preservation; D001803:Blood Transfusion; D004163:Diphosphoglyceric Acids / Q000097:blood*; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans
D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate
P-31-NMR-Untersuchungen zur In-vivo-Regeneration konservierungsbedingter Veränderungen des 2.3-Diphosphorglyzeratgehaltes roter Blutzellen.