title: Interleukin-1 beta (IL-1 beta) levels in gingival crevicular fluid from adults with previous evidence of destructive periodontitis. A cross sectional study.
abstract: We have estimated the levels of Interleukin-1 beta (IL-1 beta) by ELISA in gingival crevicular fluid (GCF) at 58 sites from 37 patients with adult periodontitis. GCF was collected for 5 s on filter papers and a 2nd sample was collected for 30 s 1 min later. 68/116 strips yielded detectable levels of IL-1 beta. IL-1 beta was present in both the 1st and 2nd samples at 28 sites, in the 1st only at 4 sites and in the 2nd only at 8 sites; 18 sites were below the level of detection for the assay. When the concentrations of IL-1 beta were calculated in the original volume of GCF on each strip, the mean value for positive strips was 34.16 +/- 29.45 (SD) pg/microliters with a range from 1.75 to 97.13 pg/microliters. There were no statistically significant correlations with the plaque index, bleeding index or probable crevice depth (pocket depth). The results indicate that IL-1 is present in the GCF from a proportion of sites with evidence of previous periodontal destruction.
authors.affiliation: Medical Research Council Dental Research Unit, London Hospital Medical College, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Wilton
authors.forename: J L
authors.initials: JL
authors.lastname: Bampton
authors.forename: G S
authors.initials: GS
authors.lastname: Griffiths
authors.forename: M A
authors.initials: MA
authors.lastname: Curtis
authors.forename: J S
authors.initials: JS
authors.lastname: Life
authors.forename: N W
authors.initials: NW
authors.lastname: Johnson
authors.forename: J R
authors.initials: JR
authors.lastname: Powell
authors.forename: G J
authors.initials: GJ
authors.lastname: Harrap
authors.forename: P
authors.initials: P
authors.lastname: Critchley
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000737:chemistry; D003430:Cross-Sectional Studies; D003774:Dental Plaque Index; D004198:Disease Susceptibility; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005884:Gingival Hemorrhage / Q000378:metabolism; D005891:Gingivitis / Q000378:metabolism / Q000503:physiopathology; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000378:metabolism / Q000503:physiopathology; D010518:Periodontitis / Q000378:metabolism* / Q000503:physiopathology; D011506:Proteins / Q000032:analysis
chemical_list: D015415:Biomarkers; D007375:Interleukin-1; D011506:Proteins