Hyperploid cells in the limb buds of rats and 5-azacytidine-induced digital deformities.
To clarify the mechanism underlying the digital teratogenesis of 5-azacytidine (5-AC), the DNA contents of mesenchymal cells were examined in the limb buds of the rat by microspectrophotometry. 5-AC induced hyperploid cells: 2.4% at 18 h after injection and 3.2% at 24 h. No hyperploid cells were noted 30 h after the treatment. Because posttreatment caffeine suppressed 5-AC-induced digital defects, the actions of caffeine on 5-AC-induced hyperploid cells were further studied; no hyperploid cells were detected at any sampling point. Caffeine suppressed hyperploid cells as well as digital defects. These results suggest that the causative factors of digital defects by 5-AC may involve hyperploid cells. However, in that there were too few hyperploid cells to cause the defects, further studies are required to clarify the nature of the action of 5-AC.
Kurishita
A
A
Department of Radiation Research, Tohoku University School of Medicine, Sendai, Japan.
Ihara
T
T
D000014:Abnormalities, Drug-Induced / Q000196:embryology / Q000209:etiology* / Q000235:genetics / Q000517:prevention & control; D000818:Animals; D001374:Azacitidine / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002110:Caffeine / Q000494:pharmacology; D005260:Female; D006614:Hindlimb / Q000002:abnormalities / Q000196:embryology; D006801:Humans; D008297:Male; D011123:Polyploidy; D051381:Rats; D011919:Rats, Inbred Strains / Q000196:embryology; D013047:Specific Pathogen-Free Organisms; D014034:Toes / Q000002:abnormalities* / Q000196:embryology
D002110:Caffeine; D001374:Azacitidine