[Effect of vitamin E on transcription in isolated nuclei and rat liver chromatin in normal status and in E-hypovitaminosis].
The effect of alpha-tocopherol on the RNA-polymerase activity in isolated rat nuclei and chromatin from normal and E-deficient rats and the possible role of tocopherol-binding proteins in this process were studied. Some differences in the RNA-polymerase activities of the nuclei were found; however, in vitro added alpha-tocopherol had no effect on the level of the label incorporation into RNA. No effect of alpha-tocopherol on this process was observed after addition of cytosol either. Analysis of chromatins from normal and E-deficient rats revealed no differences in their RNA-polymerase activities. In vitro added alpha-tocopherol increased the RNA-polymerase activity of normal (but not of vitamin E-deficient) rats. Some differences in the RNA-polymerase activities were noted after addition to the incubation medium of the Triton X-100-solubilized nuclear fraction specifically binding alpha-tocopherol. This effect was enhanced in the presence of exogenous alpha-tocopherol. The susceptibility of chromatin from normal and E-deficient rats to DNAse I hydrolysis was also found to be different. It was concluded that vitamin E can influence the RNA-polymerase activity of the nuclei and chromatin as well as the chromatin structure and that alpha-tocopherol-binding proteins are necessary for the vitamin E effect on the RNA-polymerase activity to be manifested.
Petrova
G V
GV
Kapralov
A A
AA
Donchenko
G V
GV
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects* / Q000201:enzymology; D002843:Chromatin; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D005260:Female; D006868:Hydrolysis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D012313:RNA / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*; D014544:Uridine Triphosphate / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*; D014811:Vitamin E Deficiency / Q000378:metabolism*
D002843:Chromatin; D014810:Vitamin E; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D003850:Deoxyribonuclease I; D014544:Uridine Triphosphate
Vliianie vitamina E na transkriptsiiu v izolirovannykh iadriakh i khromatine pecheni krys v norme i pri E-gipovitaminoze.