Enzymatic fragment substitution as a tool in protein design.
An easy and rapid enzymatic method is described which allows replacement of P'-residues in bovine pancreatic trypsin inhibitor. Insertion of Xaa-Arg or Xaa-Lys into a BPTI fragment lacking P1' = Ala16 and P2' = Arg17 was carried out in a "one pot" reaction catalysed by trypsin in the presence of 80% 1,4 butanediol.
Tschesche
H
H
Universität Bielefeld, Fakultät für Chemie, Lehrstuhl für Biochemie, Germany.
Groeger
C
C
Wenzel
H R
HR
D000595:Amino Acid Sequence; D000818:Animals; D007611:Aprotinin / Q000138:chemical synthesis / Q000737:chemistry*; D001665:Binding Sites; D002417:Cattle; D015195:Drug Design; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry
D010446:Peptide Fragments; D007611:Aprotinin; D014357:Trypsin