title: [Identification, isolation and physico-chemical properties of neurospecific alpha2-globulin].
abstract: In the immunization process of rabbits with the protein fraction of the water-salt extract from human brain partially and concentrated at 500-600 times was received antiserum revealed brain specific alpha 2-globulin that is not identical to the known cytoplasmatic brain specific antigens. This antigen has got electrophoretic mobility of alpha 2-globulins, molecular weight 90 +/- 10 kD and isoelectric point 4.1-5.4. Develop the procedure for purification of this antigen on the basis of the combination ion change, affinity, hydrophobic chromatography gel-filtration and isochromatofocusing.
authors.forename: V P
authors.initials: VP
authors.lastname: Chekhonin
authors.forename: E A
authors.initials: EA
authors.lastname: Koroteeva
authors.forename: G V
authors.initials: GV
authors.lastname: Morozov
authors.forename: A V
authors.initials: AV
authors.lastname: Makarov
mesh_terms: D000510:Alpha-Globulins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D001923:Brain Chemistry; D011232:Chemical Precipitation; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D004589:Electrophoresis, Disc / Q000379:methods; D006801:Humans; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000510:Alpha-Globulins; D009419:Nerve Tissue Proteins
vernacular_title: Identifikatsiia, vydelenie i fiziko-khimicheskie svoĭstva neĭrospetsificheskogo alpha2-globulina.