Counterflow affinity isotachophoresis on cellulose acetate membranes.
Counterflow isotachophoresis on cellulose acetate membranes of human alpha-fetoprotein (AFP) was performed with concanavalin A, lentil lectin, and castor bean lectin driven by electroendosmotic counterflow. This counterflow caused a uniform stream of lectin to migrate towards the cathode against AFP with carrier ampholytes in steady-state position. Retardation of microheterogeneity forms bound to lectins was observed, giving results comparable to standard crossed affinity immunoelectrophoresis. Smaller amounts of lectins and more diluted samples of AFP could be used in the described method.
Schranz
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D
Department of Pathology and Immunology of Cancer, Academy of Medicine, Poznań, Poland.
Morkowski
S
S
Karamova
E R
ER
Abelev
G I
GI
D002367:Ricinus communis; D002482:Cellulose / Q000031:analogs & derivatives*; D003208:Concanavalin A; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D007887:Fabaceae; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007202:Indicators and Reagents; D037102:Lectins; D008567:Membranes, Artificial; D037121:Plant Lectins; D010946:Plants, Medicinal; D010947:Plants, Toxic; D000509:alpha-Fetoproteins / Q000737:chemistry
D007202:Indicators and Reagents; D037102:Lectins; D008567:Membranes, Artificial; D037121:Plant Lectins; D000509:alpha-Fetoproteins; D003208:Concanavalin A; C005062:acetylcellulose; D002482:Cellulose