Construction of a map of chromosome 16 by using radiation hybrids.
A human-hamster cell hybrid carrying a single copy of chromosome 16 as the only human genetic material was irradiated with a single dose of gamma-rays (7000 rads; 1 rad = 0.01 Gy) and then fused with a thymidine kinase-deficient hamster cell line (RJKM) to generate radiation hybrids retaining unselected fragments of this human chromosome. In two experiments, 223 hybrids were isolated in hypoxanthine/aminopterine/thymidine (HAT) medium and screened with 38 DNA probes, corresponding to anonymous DNA or gene sequences localized on chromosome 16. The most likely order and location of the 38 DNA sequences were established by multiple pairwise analysis and scaled to estimate physical distance in megabases. The order and the distances thus obtained are mostly consistent with available data on genetic and physical mapping of these markers, illustrating the usefulness of radiation hybrids for mapping.
Ceccherini
I
I
Laboratorio di Genetica Molecolare, Istituto G. Gaslini, Genova, Italy.
Romeo
G
G
Lawrence
S
S
Breuning
M H
MH
Harris
P C
PC
Himmelbauer
H
H
Frischauf
A M
AM
Sutherland
G R
GR
Germino
G G
GG
Reeders
S T
ST
D002874:Chromosome Mapping / Q000379:methods*; D002885:Chromosomes, Human, Pair 16 / Q000648:ultrastructure*; D005720:Gamma Rays; D005796:Genes; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects
D005819:Genetic Markers