Cell-surface regulation of beta 1-integrin activity on developing retinal neurons.
Integrins are a family of alpha beta heterodimeric receptors that mediate cell-cell and cell-substratum interactions. Integrin binding to extracellular ligands regulates cell adhesion, shape, motility, intracellular signalling and gene expression. Mechanisms that regulate integrin function are, therefore, central to the participation of integrins in a diverse set of cellular events. Here we report the identification of TASC, a monoclonal antibody to a novel epitope on the integrin beta 1 subunit, which inhibits cell adhesion to vitronectin but promotes adhesion to laminin and collagen types I and IV. We show that developing retinal neurons that have lost responsiveness to laminin regain the ability to bind laminin in the presence of TASC. Thus, beta 1-class integrins are likely to occupy multiple affinity states that can be modulated at the cell surface.
Neugebauer
K M
KM
Howard Hughes Medical Institute, San Francisco, California 94143-0724.
Reichardt
L F
LF
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002642:Chick Embryo; D003094:Collagen / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000378:metabolism; D015151:Immunoblotting; D016023:Integrins / Q000276:immunology / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009474:Neurons / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D012160:Retina / Q000166:cytology* / Q000196:embryology; D019096:Vitronectin
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D016023:Integrins; D007797:Laminin; D010446:Peptide Fragments; D019096:Vitronectin; D003094:Collagen