Effect of demineralized bone powder on osteoblast-like cells in culture. A potential rapid quality control assay.
Demineralized bone powder (DBP) has been shown to induce osteogenesis in a variety of bone defects and extra-osseous sites. Previous investigations have been carried out in animal models which are time-consuming and expensive. We studied the effect of DBP on well-established populations of osteoblast and non-osteoblast-like cells in culture to establish an inexpensive, efficient and reliable assay for bone induction. DBP and BP (non-demineralized powder), of particle size 38-53 microns, were prepared from rat long bones. ROS (rat osteosarcoma) 17/2.8 and ROS 24/1 cell lines were subcultured weekly. For both 17/2.8 (well differentiated) and 24/1 (poorly differentiated) cells, proliferation, i.e. cell count, was significantly greater in DBP enriched medium when compared with control or medium with BP. Cell counts for wells with BP were no different from controls. The increased cell count in DBP-enriched medium was significant on days 2-5 (peak effect 2-3 days). Alkaline phosphatase production reached peak levels after day 3 when proliferation was beginning to taper off. In this study a consistent increase in osteoblast proliferation and alkaline phosphatase production under the influence of DBP was demonstrated. The tissue culture assay for proliferation must now be correlated with bone induction in vivo. In future, the method may be useful for investigating the mechanism of bone induction.
Shteyer
A
A
Department of Oral and Maxillofacial Surgery, Hadassah Medical Center, Jerusalem, Israel.
Kaban
L B
LB
Kao
R T
RT
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000502:physiology*; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D003648:Decalcification Technique; D008903:Minerals; D010006:Osteoblasts / Q000201:enzymology / Q000502:physiology*; D012516:Osteosarcoma; D011786:Quality Control; D051381:Rats; D013194:Staining and Labeling
D008903:Minerals; D000469:Alkaline Phosphatase