title: Hyperventilation does not increase alveolar surfactant phospholipids in the anesthetized rat.
abstract: Pulmonary distension elicits an increase of the surfactant secretion. Effects of hyperventilation on this same secretion are less precise since they were observed under particular experimental conditions. We report a study of the effects of hyperventilation on the phospholipid content of alveolar lining fluid in the rat. One hour's hyperventilation induced by addition of a dead space to the tracheal cannula of anesthetized rats did not affect the phospholipid content of broncho-alveolar lavage fluid collected in situ immediately after killing. Phospholipid content (4.82 +/- 1.39 mg.g-1 dry lung weight) did not differ significantly from that in anesthetized spontaneously breathing rats (4.00 +/- 1.09 mg.g-1 dry lung weight). Furthermore, phospholipid content was not found to increase in animals maintained at 37 degrees C for 20 min (4.43 +/- 1.30 mg.g-1 dry lung weight) or 60 min (3.55 +/- 0.88 mg.g-1 dry lung weight) after killing. In conclusion the constancy of phospholipid content can be due either to a normal secretion or to a hypersecretion with a concomitant removal.
authors.affiliation: Laboratoire de Physiologie, Faculté de Médecine, Toulouse, France.
authors.forename: L
authors.initials: L
authors.lastname: Brondel
authors.forename: J
authors.initials: J
authors.lastname: Rami
authors.forename: D
authors.initials: D
authors.lastname: Riviere
authors.forename: J P
authors.initials: JP
authors.lastname: Besombes
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D001992:Bronchoalveolar Lavage Fluid / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006985:Hyperventilation / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012129:Respiratory Function Tests; D014520:Urethane
chemical_list: D010743:Phospholipids; D011663:Pulmonary Surfactants; D014520:Urethane