Enzymatic dispersion of mast cells from human sinus mucosa: characterization of histamine release and its comparison with chopped fragments of the tissue.
Functional characteristics of mast cells in chopped fragments from sinus mucosa, which was dissected from patients with chronic sinusitis, were compared with those from dispersed cells prepared by enzymatic treatment. The results obtained in this study were the following. (1) Both chopped fragments and dispersed cells released histamine in a dose-dependent manner when incubated with anti-IgE. However, higher histamine release was always observed in dispersed cells. (2) Although no differences in the ability to reduce histamine release with salbutamol or forskolin could be observed between chopped fragments and dispersed cells, staurosporin and p-bromophenacyl bromide were more active on dispersed mast cells than chopped fragments. (3) Passive sensitization of dispersed cells with an allergic serum containing IgE to mite could be achieved only after elution of IgE on the cells with lactic acid.
Mita
H
H
Clinical Research Center for Rheumato-Allergology National Sagamihara Hospital, Kanagawa, Japan.
Ishii
T
T
Akiyama
K
K
Tamura
T
T
Tadokoro
K
K
Saito
H
H
Yasueda
H
H
Shida
T
T
D000098:Acetophenones / Q000494:pharmacology; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D006636:Histamine Release / Q000276:immunology*; D006801:Humans; D006838:Hydrocarbons / Q000494:pharmacology; D007073:Immunoglobulin E / Q000276:immunology; D008407:Mast Cells / Q000276:immunology*; D009297:Nasal Mucosa / Q000276:immunology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000494:pharmacology; D019311:Staurosporine
D000098:Acetophenones; D000470:Alkaloids; D006838:Hydrocarbons; D007073:Immunoglobulin E; D011493:Protein Kinase C; D019311:Staurosporine; C006821:4-bromophenacyl bromide