title: [Detection of activated oncogenes in hematological malignancies using RT-PCR method and its clinical applications].
abstract: Recently, a new and simple method of PCR has been developed and its application is extending widely including clinical research. We are using this method to detect various activated oncogenes within clinical samples from hematological malignancies. We now attempt to use these results as supporting evidences in clinical diagnosis. Here we report several successful results we have obtained through the study of activated RAS oncogenes.
authors.affiliation: Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Toyoshima
authors.forename: F
authors.initials: F
authors.lastname: Ishikawa
authors.forename: H
authors.initials: H
authors.lastname: Hirai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003062:Codon; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012194:RNA-Directed DNA Polymerase
chemical_list: D003062:Codon; D004273:DNA, Neoplasm; D012194:RNA-Directed DNA Polymerase