Removal of 3' nontranslated cDNA sequence improves interferon gamma synthesis in Escherichia coli.
The cDNA coding for human interferon gamma contains approximately 600 basepairs not translated sequence with 4 adenine/thymidine (AT)-rich clusters at its 3'end. The influence of this sequence on the expression of recombinant human Interferon gamma in Escherichia coli was investigated. Removal of the non-translated sequence and especially of the AT-rich clusters yielded an increase in expression of interferon gamma from 10 to 40% of total protein. These results may also be important for the production of other recombinant human proteins with similar 3' sequences in their cDNA.
Krehle
K
K
Fraunhofer Institute for Toxicology and Molecular Biology, Hannover, Fed. Rep. of Germany.
Böhm
J
J
Otto
B
B
D001426:Bacterial Proteins / Q000096:biosynthesis; D002478:Cells, Cultured; D004247:DNA / Q000502:physiology*; D004269:DNA, Bacterial / Q000502:physiology*; D004926:Escherichia coli / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D010957:Plasmids; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007371:Interferon-gamma; D004247:DNA