[Changes of intermediate filament during mitosis in two epithelial cell lines].
Immunofluorescence microscopy was used to follow the rearrangement of keratin filaments and vimentin filaments during mitosis in Vero and HeLa cell lines. The experiment results showed that the three dimensional organization and structure of intermediate filaments changed drastically during mitosis. The behavior of intermediate filaments was different in these two epithelial cell lines. In mitotic Vero cells the keratin filaments and vimentin filaments maintained their filamentous structure and formed a cage around the mitotic apparatus. In mitotic HeLa cells the keratin filaments and vimentin filaments reorganized extensively and formed granular cytoplasmic bodies. The ratio of granular cytoplasmic body formation changed in different mitotic phase. The interphase intermediate filament network was reconstructed after mitosis. It is proposed that the state of intermediate filament network in these cells is cell cycle-dependent and intermediate filaments may have some skeletal role in mitosis.
Cai
S T
ST
Shanghai Institute of Cell Biology, Academia Sinica.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002478:Cells, Cultured; D004847:Epithelial Cells; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000502:physiology*; D007633:Keratins / Q000276:immunology; D008938:Mitosis; D014709:Vero Cells; D014746:Vimentin / Q000276:immunology
D000906:Antibodies; D014746:Vimentin; D007633:Keratins