DNA histogram debris theory and compensation.
In this paper we describe a theory of DNA histogram debris generation and compensation that can be applied to paraffin-embedded frozen tissue preparations. The theory predicts the distribution of fragments generated from single and multiple random sectioning of three-dimensional ellipsoids representing nuclei. The fragment distribution is assumed to be a major component of the underlying debris in DNA histograms. A comparison of S-phase fractions (SPF) from matched tissue prepared by frozen and formalin-fixed paraffin-embedded DNA methods demonstrates the usefulness of the theory.
Bagwell
C B
CB
Maine Cytometry Research Institute, Verity Software House Inc., Portland.
Mayo
S W
SW
Whetstone
S D
SD
Hitchcox
S A
SA
Baker
D R
DR
Herbert
D J
DJ
Weaver
D L
DL
Jones
M A
MA
Lovett
E J
EJ
D002453:Cell Cycle; D003198:Computer Simulation; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry; D005629:Frozen Sections; D008954:Models, Biological; D009010:Monte Carlo Method; D010232:Paraffin; D013194:Staining and Labeling
D010232:Paraffin; D004247:DNA