Characterization of pore-forming activity in liver mitochondria from Anguilla anguilla. Two porins in mitochondria?
A fast purification procedure for the isolation and purification of eukaryotic porin (De Pinto et al., (1987) Biochim. Biophys. Acta 905, 499-502) was applied to liver mitochondria of the fish Anguilla anguilla. A protein preparation was obtained which formed slightly anionically selective pores in reconstitution experiments with lipid bilayer membranes. The distribution of single-channel conductances had two maxima of 2.4 nS and 4.0 nS in 1 M KCl. Sodium dodecylsulfate electrophoretograms of the protein preparation showed the presence of two bands of very similar electrophoretic mobility (32 and 32.5 kDa). Both bands cross-reacted with antibodies raised against purified bovine heart porin and with antibodies raised against the 19 amino acids N-terminal end of human porin. No cross-reactivity was observed with antibodies against yeast porin. The peptide maps of the two bands showed slight differences. The possibility of the presence of two different porins in liver mitochondria of Anguilla anguilla is discussed. An extensive immunological comparison of different mitochondrial porins is presented.
De Pinto
V
V
Dipartimento Farmaco-Biologico, Università di Bari, Italy.
Zara
V
V
Benz
R
R
Gnoni
G V
GV
Palmieri
F
F
D000811:Anguilla; D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D007473:Ion Channels / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008930:Mitochondria, Liver / Q000737:chemistry* / Q000276:immunology; D008970:Molecular Weight; D010449:Peptide Mapping; D018272:Porins; D012441:Saccharomyces cerevisiae / Q000276:immunology
D000908:Antibodies, Fungal; D001425:Bacterial Outer Membrane Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; D018272:Porins