[2-site competitive immunoenzyme analysis based on monoclonal antibodies for the detection and titration of antihemagglutinating measles antibodies].
Using monoclonal antibodies (MCA) to measles virus (strain Leningrad-16) hemagglutinin, a competitive enzyme immunoassay (cEIA) was developed for the detection and titration of antihemagglutinating antibodies in human sera. Serum samples from children, collected in measles foci, were tested in cEIA, SP-EIA, HI, and PHA tests. The results evidence a high correlation of antihemagglutinin titres in cEIA and HI tests, the correlation coefficient of cEIA and PHA being 97.7%. The cEIA based on MCA to measles virus hemagglutinin is highly specific for the detection and titration of measles antihemagglutinins, it does not involve the use of simian erythrocytes. A significant advantage of this test system consists in the possibility of using unpurified antigen prepared from the infected cell lysate.
Nagieva
F G
FG
Nikulina
V G
VG
Krasnova
N I
NI
Barkova
E P
EP
Karmanova
R I
RI
Pikhtianen
A I
AI
Andzhaparidze
O G
OG
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D001665:Binding Sites; D001667:Binding, Competitive; D002648:Child; D000939:Epitopes / Q000276:immunology; D005069:Evaluation Studies as Topic; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006389:Hemagglutinins, Viral / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008459:Measles virus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D006389:Hemagglutinins, Viral
Dvukhsaĭtovyĭ konkurentnyĭ immunofermentnyĭ analiz na osnove monoklonal'nykh antitel dlia vyiavleniia i titrovaniia antigemaggliutiniruiushchikh korevykh antitel.