Gene V protein-mediated translational regulation of the synthesis of gene II protein of the filamentous bacteriophage M13: a dispensable function of the filamentous-phage genome.
Introduction of a deletion in the genome of wild-type M13 bacteriophage that eliminates translational repression of M13 gene II by its cognate gene V protein had no effect on phage viability. Furthermore, it was noted that gene V protein of phage IKe, a distant relative of M13, does not function as a translational repressor of its cognate gene II protein. The data strongly indicate that the gene V protein-mediated control of gene II expression in bacteriophage M13 is an evolutionary relic of the ancestral filamentous-phage genome and thus dispensable for proper filamentous-phage replication.
Zaman
G J
GJ
Laboratory of Molecular Biology, University of Nijmegen, The Netherlands.
Kaan
A M
AM
Schoenmakers
J G
JG
Konings
R N
RN
D001483:Base Sequence; D002872:Chromosome Deletion; D003090:Coliphages / Q000235:genetics*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000502:physiology; D012367:RNA, Viral / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D012097:Repressor Proteins; D014764:Viral Proteins; C057816:gene 5 protein, Enterobacteria phage M13