Genotypic and phenotypic changes of a mouse lymphoma during long-term cultivation.
NK/Ly mouse lymphoma ascites cells were explanted and established in culture. The cells grew in monolayer and showed fibroblast-like morphology. The original NK/Ly cells contained one large metacentric marker chromosome, while the in vitro growing cells had two metacentrics in the early passages. These so-called bi-armed chromosomes were growing in number, up to seven, along with the number of subcultures. No tumour "take" was observed when the cells were given into mice intraperitoneally. However, a solid tumour developed following injection of cells into the leg muscle. The histological picture of this solid tumour was anaplastic sarcoma. It is concluded that the accumulation of bi-armed chromosomes, which were formed either by anaphase bridges, or by centric fusion of telocentric chromosomes, led to a profound alteration of lymphoma resulting a phenotype of anaplastic sarcoma.
Pályi
I
I
National Institute of Oncology, Budapest, Hungary.
Gál
F
F
Péter
I
I
Sugár
J
J
D000818:Animals; D001202:Ascitic Fluid; D002455:Cell Division; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure*; D005838:Genotype; D007633:Keratins / Q000032:analysis; D008223:Lymphoma / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009368:Neoplasm Transplantation; D010641:Phenotype; D012513:Sarcoma, Experimental / Q000235:genetics / Q000473:pathology; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis
D014746:Vimentin; D007633:Keratins