Expression and functional characterization of a soluble form of vascular cell adhesion molecule 1.
Vascular cell adhesion molecule 1 (VCAM1) is a leukocyte adhesion molecule induced on human endothelium in vitro and in vivo by inflammatory stimuli. A truncated cDNA for VCAM1 was constructed, stably expressed in Chinese Hamster Ovary (CHO) cells, and the secreted recombinant soluble form of VCAM1 (rsVCAM1) purified to homogeneity by immunoaffinity chromatography. Immobilized rsVCAM1 is a functional adhesion protein, and selectively binds only VLA4-expressing cells, including human B and T lymphocytes, NK cells, and certain lymphoblastoid cell lines. T cell subset analyses indicate preferential binding of CD8+ memory cells. rsVCAM1 should prove valuable for the further study of the role of VCAM1 during inflammatory and immune responses in vivo.
Lobb
R
R
Biogen Inc., Cambridge, MA 02142.
Chi-Rosso
G
G
Leone
D
D
Rosa
M
M
Newman
B
B
Luhowskyj
S
S
Osborn
L
L
Schiffer
S
S
Benjamin
C
C
Dougas
I
I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D003062:Codon; D003593:Cytoplasm / Q000502:physiology; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000502:physiology; D014162:Transfection; D019010:Vascular Cell Adhesion Molecule-1
D015815:Cell Adhesion Molecules; D003062:Codon; D011994:Recombinant Proteins; D019010:Vascular Cell Adhesion Molecule-1