Precise mapping of the rnpB gene encoding the RNA component of RNase P in Escherichia coli K-12.
In Kohara's library derived from Escherichia coli K-12 W3110 (Y. Kohara, K. Akiyama, and K. Isono, Cell 50:495-508, 1987), multiple copies of chromosomal sequence are found at 68 and at 64 to 65 min (M. Umeda and E. Ohtsubo, J. Mol. Biol. 213:229-237, 1990). We have determined that the rnpB gene (previously mapped at 70 min [B. J. Bachmann, Microbiol. Rev. 54:130-197, 1990]) is located within these segments of repeated sequences as five separate copies, together with tdcA, B, C, and R (mapped at 68 min [Bachmann, 1990]) and six unidentified open reading frames. Since close linkage of rnpB and tdc is found in various strains of E. coli K-12, the rnpB gene should be mapped at 68 min rather than 70 min.
Komine
Y
Y
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Inokuchi
H
H
D001483:Base Sequence; D004722:Endoribonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D015723:Gene Library; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D012329:RNA, Bacterial / Q000235:genetics*; D015183:Restriction Mapping; D043262:Ribonuclease P
D029968:Escherichia coli Proteins; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli