title: Functional Golgi units in microtubule-disrupted cultured atrial myocytes.
abstract: We examined the effects of disassembly of microtubules (MT) on the structure and the functions of the Golgi apparatus (GA) in cultured atrial myocytes. MT disassembly with nocodazole led to fragmentation of the GA into small units. The fragmented Golgi units retained their cis-trans polarity and post-cisternal elements, including the trans-Golgi network (TGN). Neither endocytosis of lectin-labeled membrane nor its delivery to the fragmented Golgi units was interrupted by fragmentation of the GA after MT disassembly with nocodazole treatment. A fraction of the secretory granules associated with the fragmented Golgi units was also labeled with the internalized tracer. These results suggest that in nocodazole-treated cultured atrial myocytes, the fragmented Golgi units appear to be structurally and functionally intact despite the altered geometric arrangement of the GA in the cells.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Iida
authors.forename: Y
authors.initials: Y
authors.lastname: Shibata
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis / Q000378:metabolism; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000187:drug effects; D004705:Endocytosis / Q000502:physiology; D006056:Golgi Apparatus / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D006325:Heart Atria / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D006651:Histocytochemistry; D006735:Horseradish Peroxidase / Q000378:metabolism; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000187:drug effects / Q000502:physiology*; D015739:Nocodazole / Q000494:pharmacology; D009708:Nucleotidases / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins / Q000378:metabolism
chemical_list: D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D009320:Atrial Natriuretic Factor; D006735:Horseradish Peroxidase; D009708:Nucleotidases; C025221:cytidine-5'-monophosphatase; D015739:Nocodazole