title: Modulation of a gated ion channel admittance in lipid bilayer membranes.
abstract: A future class of amperometric biosensors may utilize gated ion channels such as acetylcholine and glutamate receptors as chemical detection components. In this study, bilayer lipid membranes containing voltage-dependent anion channels (VDAC) were used to model an ion-channel-based biosensor which could continuously monitor AC amperometric changes resulting from induced changes in channel conductance. The in-phase and quadrature components of the induced alternating membrane current were monitored as a function of the applied DC offset voltage which was superimposed on the sinusoidal test voltage. The accuracy and sensitivity of the AC-measured VDAC response was dependent on the magnitude of the AC test voltage relative to the DC offset necessary for channel closure. The VDAC channel appears to be a suitable model protein for AC impedance-based biosensor fabrication.
authors.affiliation: Code 6090, Naval Research Laboratory, Washington, DC 20375.
authors.forename: D A
authors.initials: DA
authors.lastname: Stenger
authors.forename: D H
authors.initials: DH
authors.lastname: Cribbs
authors.forename: T L
authors.initials: TL
authors.lastname: Fare
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry; D007473:Ion Channels; D008051:Lipid Bilayers
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers