Study of IgE-dependent basophil releasability in allergic patients.
For this study, venous blood from 160 patients with a diagnosis of asthma and/or rhinitis was used. Histamine release test (H.R.T.) with anti-IgE at 1/5 and 1/25 dilutions and with causal antigen in the case of atopic patients (144) was carried out on all patients. Basophils from atopic patients released more histamine than those from nonatopic patients (p < 0.001). Basophils of atopic patients released more histamine with 1/5 anti-IgE dilution (p < 0.01), while non-atopic patients did so with the 1/25 dilution (p < 0.05). On grouping atopic patients according to positive or negative results in Ag-specific histamine release, 14% of patients presented negative Ag-specific H.R.T. and 85.7% of the cases did not respond to anti-IgE stimulus; this was 12% of the total number of atopics. On the other hand, 17% of the patients studied did not show positive histamine release against anti-IgE and 70.6% of them had negative Ag-specific release. As for the effect of age on IgE-dependent histamine release, the group of patients who presented greater releasability corresponded to those older than 6 years of age. The discrepancies observed between the clinical history, skin tests, serum IgE and antigen-dependent histamine release in some patients could be related to the individual basophil ability to release histamine. Therefore, this basophil releasability evaluation has an important practical application in discerning false negatives in antigen-specific H.R.T.
Sanz
M L
ML
Departamento de Alergología e Inmunología Clínica, Clínica Universitario, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain.
Latasa
M
M
García
B E
BE
Gato
J J
JJ
Oehling
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D000941:Antigens; D001249:Asthma / Q000276:immunology; D001491:Basophils / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006636:Histamine Release; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood*; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D007073:Immunoglobulin E