title: A general procedure for evaluation of immunological relevance of synthetic peptides: peptides synthesized on paper in enzyme-linked immunosorbent assay.
abstract: Multiple continuous-flow solid-phase peptide synthesis has been adapted for synthesis of peptides on a cellulose carrier (Whatman 3MM paper). Paper-bound synthetic peptides that represent antigenic determinants of particular proteins detected antibodies against the respective proteins in an enzyme-linked immunosorbent assay. The method is applied to the synthesis, and use in site-directed serology, of four peptides derived from the gp41 glycoprotein of HIV, the Epstein-Barr virus-determined nuclear antigen-1 and VCA proteins of the Epstein-Barr virus, and the early region of human papillomavirus type 11.
authors.affiliation: Research Institute for Feed Supplements and Veterinary Drugs, Jílové u Prahy, Czechoslovakia.
authors.forename: V
authors.initials: V
authors.lastname: Krchnák
authors.forename: J
authors.initials: J
authors.lastname: Vágner
authors.forename: J
authors.initials: J
authors.lastname: Novák
authors.forename: A
authors.initials: A
authors.lastname: Suchánková
authors.forename: J
authors.initials: J
authors.lastname: Roubal
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000032:analysis; D002482:Cellulose; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000276:immunology; D012699:Serology / Q000379:methods
chemical_list: D000906:Antibodies; D000939:Epitopes; D010455:Peptides; D002482:Cellulose