title: Effect of interleukin 3 on the differentiation and histamine content of cultured bone marrow mast cells.
abstract: Mouse bone marrow hematopoietic stem cells were isolated from mouse femur bone and cultured in RPMI 1640 supplemented medium with 20 units/ml of the purified T-cell lymphokine, interleukin 3 (IL-3), IL-3 was uniquely able to induce the proliferation and differentiation of mature mast cells in vitro. The sparse granulation of the bone marrow-derived mast cells (BMMC) can be seen by day 5, progressing to definable mast cells by day 7, the mast cells appear morphologically mature and comprise a 96% pure population after 14 days of the culture. The monocytes macrophages, eosinophils and neutrophils disappeared by day 9. After 4 weeks of tissue culture, mast cells are fully mature and completely granulated at 98% cell purity. The BMMC are mononuclear, oval or round in shape and appear smaller than rat peritoneal mast cells. BMMC are stable over 3-5 months in conditioned medium. The homogeneous mast cell population possesses membrane receptors and mediators, such as histamine in their metachromatic granules. The histamine content of BMMC in culture between 2 to 4 weeks rose from 1.43 to 1.82 pg/cell. Moreover, the percentage of histamine release caused by 0.1 microM and 1.0 microM ionophore A23187 was 15% and 35%, respectively. By contrast, the histamine releasing activity of 0.01% and 0.001% compound 48/80 were 12 +/- 2% and 59 +/- 7% respectively. The granular density, histamine content and histamine release activity of BMMC are different from that of peritoneal mast cells.
authors.affiliation: Department of Pharmacology, Kaohsiung Medical College, Taiwan, R.O.C.
authors.forename: H F
authors.initials: HF
authors.lastname: Chiu
authors.forename: B A
authors.initials: BA
authors.lastname: Burrall
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D000001:Calcimycin / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006632:Histamine / Q000378:metabolism*; D006636:Histamine Release / Q000187:drug effects; D007377:Interleukin-3 / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D007377:Interleukin-3; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine