Autosomal recessive polycystic kidney in rats.
We evaluated the characteristics of renal lesions in rat autosomal recessive polycystic kidney (ARPK). In rat ARPK, small cysts appeared primarily in the medulla 2 months after birth and gradually extended to the cortex, forming large cysts involving the entire layer after 8 months. By immunofluorescence microscopy, type IV collagen was more strongly stained in the epithelial basement membrane of the rat ARPK than in the normal rat tubular basement membrane (TBM). Electron microscopy demonstrated a marked thickening, slight splitting and lamination of the TBM in the ARPK. As peroxidase-labeled lectins, dolichos biflorus very strongly stained the cyst epithelium whereas lens culinaris did not. These findings indicate that cysts in rat ARPK originate in the collecting duct.
Inage
Z
Z
Department of Pediatrics, Nihon University, School of Medicine, Tokyo, Japan.
Kikkawa
Y
Y
Minato
M
M
Owada
M
M
Kitagawa
T
T
Ohno
K
K
Kondo
K
K
Ueda
Y
Y
Iidaka
K
K
D000367:Age Factors; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000473:pathology; D008854:Microscopy, Electron; D017044:Polycystic Kidney, Autosomal Recessive / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011922:Rats, Mutant Strains; D013194:Staining and Labeling
D003094:Collagen