Selective inhibition by magnosalin and magnoshinin, compounds from 'shin-i' (Flos magnoliae), of adjuvant-induced angiogenesis and granuloma formation in the mouse pouch.
Inhibitory effects of magnosalin and magnoshinin, compounds from the crude drug 'Shin-i' (Flos magnoliae), on angiogenesis and pouch granuloma formation induced by an adjuvant containing croton oil were investigated. Magnosalin inhibited angiogenesis 2.4-fold (intra-pouch) and 9.7-fold (intraperitoneal) more strongly than granuloma formation. The inhibition of angiogenesis by magnosalin was 5-fold (intra-pouch) and 21-fold (intraperitoneal) weaker than that by hydrocortisone. In contrast, intraperitoneal magnoshinin inhibited granuloma formation 2.5-fold more strongly than angiogenesis. The regression coefficients of anti-angiogenesis vs. the inhibition of granuloma formation were 1.79 for magnosalin, 1.11 for hydrocortisone, and 0.61 for magnoshinin. These results show that the anti-chronic inflammatory effect of 'Shin-i' was caused by selective inhibition of angiogenesis by magnosalin and of granuloma formation by magnoshinin.
Kimura
M
M
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Kobayashi
S
S
Luo
B
B
Kimura
I
I
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D003503:Cyclobutanes / Q000494:pharmacology*; D006099:Granuloma / Q000139:chemically induced / Q000517:prevention & control*; D007274:Injections, Intraperitoneal; D017705:Lignans; D008297:Male; D051379:Mice; D009284:Naphthols / Q000494:pharmacology*; D009389:Neovascularization, Pathologic; D010532:Peritoneal Diseases / Q000139:chemically induced / Q000517:prevention & control; D010537:Peritoneum / Q000098:blood supply; D010946:Plants, Medicinal; D012867:Skin / Q000098:blood supply; D012871:Skin Diseases / Q000139:chemically induced / Q000517:prevention & control*
D000276:Adjuvants, Immunologic; D000894:Anti-Inflammatory Agents, Non-Steroidal; D003503:Cyclobutanes; D017705:Lignans; D009284:Naphthols; C069674:magnosalin; C069675:magnoshinin