Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA.
cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.
Kroll
B
B
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
Emde
M
M
Jeromin
A
A
Penner
L
L
Rechkemmer
G
G
Kretzschmar
T
T
Klos
A
A
Köhl
J
J
Bautsch
W
W
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015936:Complement C5a / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology*; D012313:RNA / Q000235:genetics*; D018075:RNA, Complementary; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D014982:Xenopus laevis / Q000235:genetics*
D018075:RNA, Complementary; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement; D011994:Recombinant Proteins; D012313:RNA; D015936:Complement C5a