A fast staining method for CNS slices.
We describe a staining technique for quick observation of the microanatomy of the vertebrate CNS. Successive soaking of the sections in tannic acid and ferric chloride produces a black colour in the gray matter. The procedure takes 5 min and is useful for identifying electrode penetrations, localization of lesions or teaching neuroanatomy.
Riboni
L
L
Departamento de Ciencias Fisiológicas, Universidad Autónoma de Puebla, Mexico.
Luna
F
F
Núñez-Durán
H
H
D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D002712:Chlorides; D005290:Ferric Compounds; D047348:Hydrolyzable Tannins; D008322:Mammals / Q000033:anatomy & histology; D008867:Microtomy; D009419:Nerve Tissue Proteins; D011898:Ranidae / Q000033:anatomy & histology; D013194:Staining and Labeling / Q000379:methods*
D002712:Chlorides; D005290:Ferric Compounds; D047348:Hydrolyzable Tannins; D009419:Nerve Tissue Proteins; C024555:ferric chloride