Induction of cell cycle-dependent genes during cell cycle progression of arterial smooth muscle cells in culture.
Serum stimulation of arterial smooth muscle cells in culture induces a progression through the cell cycle and cell proliferation. Most genes previously described as cell cycle-dependent in various cell types also demonstrate a cell cycle-dependent expression in arterial smooth muscle cells. As in other cell types, these genes can be classified into three groups according to their mode of expression: "immediate early" genes (c-fos, c-myc, ...), "delayed early" genes (2F1, ...), and "late-G1" genes (proliferating cell nuclear antigen, thymidine kinase, . . .). In addition to these previously described genes, three genes isolated from a cDNA library of stimulated smooth muscle cells have been demonstrated to be cell cycle-dependent: A21, the rat JE gene, and L51 can be classified as "immediate early" genes, while M11 represents a new member of the "delayed early" gene family.
Gadeau
A P
AP
INSERM U8, Pessac, France.
Campan
M
M
Desgranges
C
C
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002478:Cells, Cultured; D005434:Flow Cytometry; D016193:G1 Phase / Q000502:physiology; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology*; D005796:Genes / Q000235:genetics / Q000502:physiology*; D015151:Immunoblotting; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology; D012313:RNA / Q000302:isolation & purification; D051381:Rats
D012313:RNA