title: rRNA transcription rate in Escherichia coli.
abstract: The rate of in vivo transcription elongation for Escherichia coli rRNA operons was determined by electron microscopy following addition of rifampin to log-phase cultures. Direct observation of RNA polymerase positions along rRNA operons 30, 40, and 70 s after inhibition of transcription initiation yielded a transcription elongation rate of 42 nucleotides per s.
authors.affiliation: Department of Biology, University of Virginia, Charlottesville 22901.
authors.forename: S L
authors.initials: SL
authors.lastname: Gotta
authors.forename: O L
authors.initials: OL
authors.lastname: Miller
authors.forename: S L
authors.initials: SL
authors.lastname: French
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000187:drug effects / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D007700:Kinetics; D008854:Microscopy, Electron; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics*; D012407:rRNA Operon / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin