Isolation and characterization of nitrate reductase-deficient mutants in tomato (Lycopersicon esculentum Mill.).
Five nitrate reductase-deficient mutants of tomato were isolated from an M2 population after ethylmethanesulphonate (EMS) seed treatment by means of selection for chlorate resistance. All mutations were monogenic and recessive and complementation analysis revealed that they were non-allelic. Biochemical and molecular characterization of these mutants showed that four of them are cofactor mutants while one is an apoenzyme mutant.
Schoenmakers
H C
HC
Dept. of Genetics, Agricultural University Wageningen, The Netherlands.
Koornneef
M
M
Alefs
S J
SJ
Gerrits
W F
WF
van der Kop
D
D
Chérel
I
I
Caboche
M
M
D001345:Autoradiography; D015152:Blotting, Northern; D002704:Chlorates / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005638:Fruit / Q000201:enzymology* / Q000235:genetics; D007120:Immunochemistry; D009154:Mutation; D009245:NADH Dehydrogenase / Q000378:metabolism; D009565:Nitrate Reductases / Q000235:genetics*; D012313:RNA / Q000235:genetics
D002704:Chlorates; D012313:RNA; D005020:Ethyl Methanesulfonate; D009245:NADH Dehydrogenase; D009565:Nitrate Reductases