A rapid method for purification of synthetic oligoribonucleotides.
A procedure for large-scale purification of synthetic oligoribonucleotides has been developed that has significant advantages over gel purification techniques currently in use. Synthesis was performed using commercially available 2'-O-silylated ribonucleoside 3'-O-phosphoramidites, and coupling efficiencies were consistently greater than 97% for oligoribonucleotides up to 31 residues in length. Using C4 reverse-phase chromatography to remove material not deprotected by treatment with tetrabutylammonium fluoride, we have eliminated reactants in which the 2'-O-silyl group is only partly removed, thus ensuring a homogeneous population of oligoribonucleotide.
Webster
K R
KR
Yale University School of Medicine, New Haven, CT 06510-0824.
Shamoo
Y
Y
Konigsberg
W
W
Spicer
E K
EK
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D003902:Detergents; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification*; D000644:Quaternary Ammonium Compounds; D012313:RNA / Q000302:isolation & purification; D014764:Viral Proteins
D003902:Detergents; D009843:Oligoribonucleotides; D000644:Quaternary Ammonium Compounds; C066700:RegA protein, Enterobacteria phage T4; D014764:Viral Proteins; D012313:RNA; C009405:tetrabutylammonium