title: Dynamics of a human seminal vesicle specific protein.
abstract: The present paper is concerned with the temporal alterations and tissue localization of a seminal antigen secreted by the human seminal vesicle. This antigen is recognized by antibody MHS-5, which is one of a set produced in mice by immunization with human sperm. The respective clone produced an antibody of the IgG1 subtype, which reacted with seminal fluid from over 400 normal donors and 21 semen samples from vasectomized men. Incubation of seminal vesicle secretion with either prostatic fluid or prostate specific antigen (PSA) resulted in degradation on the antigen. The experiments showed that MHS-5 antigen is a substrate for the serine protease PSA: Immunohistochemical studies suggested that MHS-5 is a "sperm-coating" antigen and is exclusively synthesized and secreted by the seminal vesicle.
authors.affiliation: Department of Anatomy & Cell Biology, University of Virginia School of Medicine, Charlottesville.
authors.forename: C J
authors.initials: CJ
authors.lastname: Flickinger
authors.forename: J C
authors.initials: JC
authors.lastname: Herr
authors.forename: R S
authors.initials: RS
authors.lastname: McGee
authors.forename: M
authors.initials: M
authors.lastname: Sigman
authors.forename: R J
authors.initials: RJ
authors.lastname: Evans
authors.forename: W M
authors.initials: WM
authors.lastname: Sutherland
authors.forename: T A
authors.initials: TA
authors.lastname: Summers
authors.forename: D R
authors.initials: DR
authors.lastname: Spell
authors.forename: D J
authors.initials: DJ
authors.lastname: Conklin
mesh_terms: D000941:Antigens / Q000032:analysis / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000378:metabolism; D006801:Humans; D008297:Male; D017430:Prostate-Specific Antigen; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D012661:Semen / Q000737:chemistry; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D013094:Spermatozoa / Q000737:chemistry
chemical_list: D000941:Antigens; D000951:Antigens, Neoplasm; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D017430:Prostate-Specific Antigen