A simple assay for proteolysis of IGFBP-3.
An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125IhIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37 degrees in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associated protease is Ca++ dependent. The substrate is also proteolyzed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.
Lamson
G
G
Department of Pediatrics, Stanford University Medical Center, California 94305.
Giudice
L C
LC
Rosenfeld
R G
RG
D000818:Animals; D001345:Autoradiography; D002352:Carrier Proteins / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D018969:Insulin-Like Growth Factor Binding Proteins; D051379:Mice; D010447:Peptide Hydrolases / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D011994:Recombinant Proteins; D013002:Somatomedins / Q000378:metabolism
D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D011994:Recombinant Proteins; D013002:Somatomedins; D010447:Peptide Hydrolases