A mechanosensitive channel in whole cells and in membrane patches of the fungus Uromyces.
Bean leaf stomata provide a topographical signal that induces germlings of the phytopathogen Uromyces appendiculatus to develop specialized infection structures. Protoplasts from germ tubes of this fungus, when examined with patch-clamp electrodes, displayed the activities of a 600-picosiemen mechanosensitive ion channel. This channel passes a variety of cations, including Ca2+, and is blocked by Gd3+ at 50 micromolar. This channel could transduce the membrane stress induced by the leaf topography into an influx of ions, including Ca2+, that may trigger differentiation.
Zhou
X L
XL
Laboratory of Molecular Biology, University of Wisconsin, Madison 53706.
Stumpf
M A
MA
Hoch
H C
HC
Kung
C
C
D001464:Barium / Q000494:pharmacology; D001487:Basidiomycota / Q000502:physiology* / Q000648:ultrastructure; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D005682:Gadolinium / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008465:Mechanoreceptors / Q000502:physiology; D008564:Membrane Potentials; D011312:Pressure; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D007473:Ion Channels; D013757:Tetraethylammonium Compounds; D001464:Barium; D019789:Tetraethylammonium; D005682:Gadolinium