title: Relationship between DNA methylation and cell proliferation in Trypanosoma cruzi.
abstract: 5-Azacytidine treatment of T. cruzi epimastigotes in culture induces active cell proliferation. This effect was detected as an increase in the cell number and [3H-methyl]thymidine incorporation into DNA. 5-Azacytidine does not alter other metabolic parameters. We have previously demonstrated that 5-azacytidine induces DNA hypomethylation in T. cruzi. Accordingly, we suggest that this chemical modification may be related to the control of T. cruzi cell division.
authors.affiliation: Department of Cell Biology and Genetics, School of Medicine, University of Chile, Santiago.
authors.forename: M V
authors.initials: MV
authors.lastname: Rojas
authors.forename: N
authors.initials: N
authors.lastname: Galanti
mesh_terms: D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA; D004261:DNA Replication / Q000187:drug effects; D016054:DNA, Protozoan / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008745:Methylation; D010101:Oxygen Consumption / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D016054:DNA, Protozoan; D004247:DNA; D001374:Azacitidine; D013936:Thymidine