Immunoalkaline phosphatase with cerium-based cytochemistry for double staining at the transmission electron microscopic level.
Immunoelectron microscopic localization of surface antigens on lymphocytes is possible using alkaline phosphatase combined with cerium-based cytochemical methods. Distinctive electron-dense deposits are easily identified at sites of antibody binding. Mouse splenocytes showing surface immunoglobulin localization and human peripheral blood lymphocytes stained for the MHC-Class II antigen HLA-DR illustrate the results. Double staining for murine Ia antigen by the alkaline phosphatase procedure, combined with immunogold labeling of antigens identified on dendritic cells, i.e., NLDC-145, demonstrates the utility of the cerium cytochemical method.
Rowden
G
G
Pathology Department, Dalhousie University, Halifax, Nova Scotia, Canada.
Dean
S
S
D000469:Alkaline Phosphatase / Q000276:immunology*; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002563:Cerium; D006801:Humans; D007583:Jejunum / Q000201:enzymology / Q000648:ultrastructure; D008214:Lymphocytes / Q000201:enzymology / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D016253:Microscopy, Immunoelectron / Q000379:methods*; D013154:Spleen / Q000201:enzymology / Q000648:ultrastructure; D013194:Staining and Labeling
D000954:Antigens, Surface; D002563:Cerium; D000469:Alkaline Phosphatase