title: Expression, purification, and crystallization of the HIV-1 reverse transcriptase (RT).
abstract: The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.
authors.affiliation: Department of Molecular Biology, Uppsala University, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Unge
authors.forename: H
authors.initials: H
authors.lastname: Ahola
authors.forename: R
authors.initials: R
authors.lastname: Bhikhabhai
authors.forename: K
authors.initials: K
authors.lastname: Bäckbro
authors.forename: S
authors.initials: S
authors.lastname: Lövgren
authors.forename: E M
authors.initials: EM
authors.lastname: Fenyö
authors.forename: A
authors.initials: A
authors.lastname: Honigman
authors.forename: A
authors.initials: A
authors.lastname: Panet
authors.forename: J S
authors.initials: JS
authors.lastname: Gronowitz
authors.forename: B
authors.initials: B
authors.lastname: Strandberg
mesh_terms: D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015751:Genes, pol; D015497:HIV-1 / Q000201:enzymology* / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D012194:RNA-Directed DNA Polymerase