title: Effect of subarachnoid hemorrhage on serotonin uptake and metabolism in rabbit basilar artery.
abstract: The effects of subarachnoid hemorrhage (SAH) on neuronal uptake and metabolism of serotonin (5-HT) in the rabbit basilar artery were examined. Extracted 3H-amines from the isolated arteries after incubation with [3H]5-HT were separated by column chromatography. Radioactivity of 5-HT and 5-hydroxyindoleacetic acid was, respectively, 52.7 +/- 13.9 and 22.9 +/- 5.4 x 10(2) dpm/mg tissue in the control group (n = 8); 32 and 18% of control after denervation (n = 6); 99 and 12% of control after treatment with pargyline (n = 7); and 65 and 76% of control after SAH (n = 7). These results suggest that the neuronal uptake of 5-HT is impaired by SAH, although monoamine oxidase activity is relatively preserved.
authors.affiliation: Department of Neurological Surgery, University of Virginia Health Sciences Center, Charlottesville 22908.
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: N F
authors.initials: NF
authors.lastname: Kassell
authors.forename: T
authors.initials: T
authors.lastname: Machi
authors.forename: D A
authors.initials: DA
authors.lastname: Dougherty
mesh_terms: D000818:Animals; D001345:Autoradiography; D001488:Basilar Artery / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D003714:Denervation; D006892:Hydroxydopamines / Q000494:pharmacology; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000378:metabolism; D016627:Oxidopamine; D010293:Pargyline / Q000494:pharmacology; D011817:Rabbits; D012701:Serotonin / Q000378:metabolism*; D013345:Subarachnoid Hemorrhage / Q000378:metabolism*
chemical_list: D006892:Hydroxydopamines; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D016627:Oxidopamine; D010293:Pargyline