Characterization of murine monoclonal antibodies that recognize defined epitopes of pertussis toxin and neutralize its toxic effect on Chinese hamster ovary cells.
Three murine monoclonal antibodies (MAb), E19, E205, and E251, raised against pertussis toxin reacted in Western blots (immunoblots) with the S1, S4, and S2-S3 subunits, respectively, and neutralized the Chinese hamster ovary cell-clustering activity of pertussis toxin. MAb E251 recognized a linear synthetic peptide corresponding to amino acids 107 to 120 of the S2 subunit, suggesting a role for this region in receptor binding.
Walker
M J
MJ
Department of Microbiology, GBF-National Research Centre for Biotechnology, Braunschweig, Germany.
Wehland
J
J
Timmis
K N
KN
Raupach
B
B
Schmidt
M A
MA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000992:Antitoxins / Q000276:immunology*; D015153:Blotting, Western; D016466:CHO Cells; D006224:Cricetinae; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D066298:In Vitro Techniques; D051379:Mice; D037342:Pertussis Toxin; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000276:immunology* / Q000633:toxicity
D000911:Antibodies, Monoclonal; D000992:Antitoxins; D000939:Epitopes; D010566:Virulence Factors, Bordetella; D011065:Poly(ADP-ribose) Polymerases; D037342:Pertussis Toxin