Reduced NMDA receptor-ion channel function in the vitamin B-6 restricted neonatal rat brain.
We have measured the binding of [3H]-MK-801 to cortical and hippocampal membrane preparations from the vitamin B-6 restricted neonatal rat. The results indicate a reduced potency and efficacy of glutamate and glycine in enhancing [3H]-MK-801 binding to the N-methyl-D-aspartate (NMDA) receptor-ion channel. Scatchard analysis of [3H]-MK-801 binding isotherms showed a significantly lower number of [3H]-MK-801 binding sites in the cortex and hippocampus of vitamin B-6 restricted rats. These results indicate a reduced function of the NMDA receptor-ion channel in the brain of these animals.
Guilarte
T R
TR
Department of Environmental Health Sciences, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21025.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001923:Brain Chemistry / Q000502:physiology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000378:metabolism; D005260:Female; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology; D007700:Kinetics; D016202:N-Methylaspartate / Q000494:pharmacology; D016318:Quisqualic Acid / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D026681:Vitamin B 6 Deficiency / Q000378:metabolism*
D007473:Ion Channels; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D016318:Quisqualic Acid; D007608:Kainic Acid