Isolation and characterization of monoclonal antibody directed against bovine alpha s2-casein.
A monoclonal antibody 62-1A of isotype IgM, directed against bovine alpha s2-casein, was isolated and characterized. Monoclonal antibody 62-1A recognized bovine alpha s2-11P- and alpha s2-9P-caseins by indirect solid phase radioimmunoassay and Western blot analysis. Crossreactivity toward native genetic variants (A, B, and C) of alpha s1-casein was similar but lower (approximately 60 to 70%) than that for alpha s2-11P-casein). Little or no crossreactivity was observed for other bovine milk or serum proteins. Antibody affinity for alpha s2-11P-casein was 1.3 x 10(9)/M. As little as 25 ng/ml (.5 ng/well) of alpha s2-11P-casein was detected by solid phase radioimmunoassay.
Leung
C T
CT
Specialized Center for Cancer Research and Education, University of Illinois School of Medicine, Chicago 60612.
Kuzmanoff
K M
KM
Beattie
C W
CW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D015153:Blotting, Western; D002364:Caseins / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005260:Female; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D008892:Milk / Q000276:immunology*
D000911:Antibodies, Monoclonal; D002364:Caseins; D000939:Epitopes