title: Appearance of the Na(+)-motive terminal oxidase in Bacillus FTU grown under three different conditions lowering the delta mu H+ level.
abstract: The terminal oxidases and coupled Na+ transport have been studied in intact cells and inside-out subcellucar vesicles of alkalo- and halotolerant Bacillus FTU grown under different conditions. Cells grown at pH 7.5 are shown to possess a system of respiration-dependent Na+ transport which is (i) inhibited by protonophorous uncoupler and (ii) activated by the delta psi-discharging agent valinomycin, suggesting that the Na+ transport is due to cooperation of the H(+)-motive oxidase and Na+/H+ antiporter. On the other hand, growth under conditions lowering the delta mu H+ level, namely (i) pH 8.6, (ii) pH 7.5 in the presence of protonophore, and (iii) pH 7.5 in the presence of low cyanide concentrations results in appearance of terminal oxidase-supported Na+ transport which is stimulated by protonophores (the Na(+)-motive oxidase). In all three cases, the appearance of ascorbate (+ TMPD) oxidation resistant to low and sensitive to high cyanide concentrations was found to occur. It is concluded that not only alkaline pH but also other conditions which lower delta mu H+ can cause substitution of Na+ for H+ as a coupling ion.
authors.affiliation: Department of Bioenergetics, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, USSR.
authors.forename: A L
authors.initials: AL
authors.lastname: Semeykina
authors.forename: V P
authors.initials: VP
authors.lastname: Skulachev
mesh_terms: D001407:Bacillus / Q000187:drug effects / Q000201:enzymology*; D001692:Biological Transport; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002412:Cations; D003486:Cyanides / Q000494:pharmacology; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010088:Oxidoreductases / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010416:Pentachlorophenol / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*
chemical_list: D002412:Cations; D003486:Cyanides; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D006859:Hydrogen; D012964:Sodium; D010416:Pentachlorophenol; D010088:Oxidoreductases; D010100:Oxygen