Galanin inhibits somatostatin release by the rat islet cell tumor in culture, Rin-m.
Using a rat islet cell tumor in culture, Rin-m, we studied the effects of the neuropeptide, galanin, on somatostatin release. Galanin applied to the incubation medium inhibited pancreatic hormone release rapidly and dose dependently with an IC50 at 4 nM and the maximal effect (40% inhibition) was elicited by 100 nM peptide. Pretreatment of Rin-m cells with pertussis toxin abolished the inhibitory effect of galanin on somatostatin release. The results suggest that galanin probably controls the function of the pancreatic delta cell through a pertussis toxin-sensitive pathway.
Amiranoff
B
B
Unité de Recherches sur la Différenciation et la Neuroendocrinologie de Cellules Digestives, INSERM U178, Villejuif, France.
Lorinet
A M
AM
Laburthe
M
M
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D007516:Adenoma, Islet Cell / Q000378:metabolism*; D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D001120:Arginine / Q000494:pharmacology; D003864:Depression, Chemical; D019004:Galanin; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D037342:Pertussis Toxin; D013004:Somatostatin / Q000378:metabolism*; D013552:Swine; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D010455:Peptides; D010566:Virulence Factors, Bordetella; D013004:Somatostatin; D019004:Galanin; D007611:Aprotinin; D001120:Arginine; D037342:Pertussis Toxin; D015056:1-Methyl-3-isobutylxanthine