Interaction of HIV-1 with susceptible lymphoblastoid cells. 1H NMR studies.
Different strains of HIV susceptible lymphoblastoid cells have been infected by HIV-1 and examined by means of 1H NMR spectroscopy at different times after infection, taking advantage of the presence of high resolution lipid signals from the plasma membrane of tumor cells. A transient decrease in intensity of fatty acid signals, originated by changes in membrane structure, has been observed early after viral infection. Marked alterations in membrane-dependent steps of phospholipid synthesis can also be inferred by the observed transient depression in peaks from choline-based metabolites. Spectral modifications deriving from changes in lipid metabolism are also produced both in infected cells a few days after infection and in permanently infected cells. 1H NMR can, therefore, monitor structural and metabolic effects induced by HIV infection.
Luciani
A M
AM
Laboratorio di Fisica e INFN Sez. Sanita, Roma, Italy.
Rosi
A
A
Maggiorella
M T
MT
Federico
M
M
Sulli
N
N
Verani
P
P
Rossi
G B
GB
Viti
V
V
Guidoni
L
L
D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D005227:Fatty Acids / Q000737:chemistry; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism; D009000:Monocytes / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014407:Tumor Cells, Cultured
D005227:Fatty Acids; D008563:Membrane Lipids; D012194:RNA-Directed DNA Polymerase