Immunohistochemical evaluation of reverse transcriptase in breast carcinoma with polyclonal antibodies raised in rabbit.
In this study, the presence of reverse transcriptase in breast tumours was examined with immunoperoxidase staining using antibodies raised in rabbit against reverse transcriptase of Moloney murine leukemia virus and against reverse transcriptase of avian myeloblastosis virus. The specificity of such antibodies was investigated with ELISA and Western blotting techniques. Five cases of infiltrating ductal carcinomas were found positive with the immune serum anti-reverse transcriptase of Moloney murine leukemia virus on 28 studied infiltrating ductal carcinomas, 2 infiltrating lobular carcinomas and 2 fibroadenomas.
Moyret
C F
CF
Laboratoire d'Immunologie et de Cancérologie, Centre Jean Perrin, Clermont-Ferrand, France.
Bernard
D J
DJ
Maurizis
J C
JC
Chassagne
J
J
de Latour
M
M
Betail
G
G
Plagne
R
R
Chollet
P
P
D000232:Adenofibroma / Q000201:enzymology* / Q000382:microbiology; D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D009189:Avian Myeloblastosis Virus / Q000201:enzymology / Q000276:immunology; D001943:Breast Neoplasms / Q000201:enzymology* / Q000382:microbiology; D002277:Carcinoma / Q000201:enzymology* / Q000382:microbiology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000201:enzymology* / Q000382:microbiology; D003593:Cytoplasm / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D008979:Moloney murine leukemia virus / Q000201:enzymology / Q000276:immunology; D009363:Neoplasm Proteins / Q000032:analysis*; D010802:Phylogeny; D012194:RNA-Directed DNA Polymerase / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D012190:Retroviridae / Q000302:isolation & purification; D018894:Reverse Transcriptase Inhibitors
D000906:Antibodies; D009363:Neoplasm Proteins; D018894:Reverse Transcriptase Inhibitors; D012194:RNA-Directed DNA Polymerase