Analysis of CD1 molecules on haematological malignancies of myeloid and lymphoid origin. I. Cell surface antigen expression.
One hundred and ninety well-characterized acute and chronic leukaemias were studied for the expression of CD1a antigen by indirect immunofluorescence (IIF). CD1a was detected on 28 per cent of mature B cell lymphoproliferative disorders, 26 per cent of acute non-lymphoblastic leukaemias (ANLL), 21 per cent of chronic granulocytic leukaemias in blast crisis (CML-BC), 53 percent of T acute lymphocytic leukaemias (T-ALL) and in only one out of 35 common acute lymphoblastic leukaemias (c-ALL). In some cases the expression of the CD1a antigen on the surface of leukaemic cells showed a spontaneous fluctuation after a short period of incubation in vitro. CD1b and CD1c molecules were also detected on B cells and acute non-lymphoblastic leukaemias. The presence of CD1 antigens was confirmed using a dot blot assay (DBA) on the lysate of leukaemic cells.
Salamone
M C
MC
Laboratorio de Immunogenetica, Hospital de Clinicas Jose de San Martin, Buenos Aires, Argentina.
Roisman
F R
FR
Santiago
J
J
Satz
M L
ML
Fainboim
L
L
D015703:Antigens, CD / Q000032:analysis / Q000145:classification; D018949:Antigens, CD1; D000943:Antigens, Differentiation / Q000032:analysis* / Q000145:classification; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000145:classification; D006801:Humans; D007938:Leukemia / Q000276:immunology*; D015448:Leukemia, B-Cell / Q000276:immunology; D015465:Leukemia, Myeloid, Accelerated Phase / Q000276:immunology; D015470:Leukemia, Myeloid, Acute / Q000276:immunology; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000276:immunology
D015703:Antigens, CD; D018949:Antigens, CD1; D000943:Antigens, Differentiation; D014408:Biomarkers, Tumor