[Interaction of rabbit IgG with anti-IgG: localization of epitopes and absence of immunoreactivity of the pFc'-fragment].
To localize essential epitopes of rabbit IgG, a series of proteolytic IgG fragments obtained by papain (Fab, Fc) or pepsin (pFc', F(ab')2) proteolysis have been prepared and their interaction with sheep antibodies against rabbit IgG has been studied. The data obtained suggest that essential immunoreactive epitopes of rabbit IgG are located in the CH2 domain and hinge region. This finding is in line with the results obtained by computing the antigenic sites of immunoglobulins. However, the deviation from the computed antigenic structure was deduced from the complete lack of immunoreactivity of the pFc fragment, it being a dimer of the terminal CH3 domain of the Fc fragment. The hinge region comparable in size with the dimensions of the epitope reveals high affinity binding to anti-IgG, thus testifying to the localization of the expressed epitope or its essential part in the hinge region. Proteolytic cleavage of this region leads to a significant decrease in the binding of the IgG fragment to anti-IgG. In addition to the CH2 domain and hinge region, a relatively low interaction of the antigen-binding antibody fragments with anti-IgG was found.
Kravchuk
Z I
ZI
Zhorov
O V
OV
Preĭgerzon
V A
VA
Zakharova
N A
NA
Shakhanina
K L
KL
Martsev
S P
SP
D000818:Animals; D002850:Chromatography, Gel; D000939:Epitopes / Q000276:immunology*; D006868:Hydrolysis; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism*; D010206:Papain / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D011817:Rabbits
D000939:Epitopes; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D010206:Papain; D010434:Pepsin A
Vzaimodeĭstvie IgG krolika s anti-IgG: lokalizatsiia épitopov i otsutstvie immunoreaktivnosti pFc'-fragmenta.