Effects of selenium on RNA synthesis and content in rat pancreas.
In order to investigate the effect of selenium supplementation on RNA in the rat pancreas, the rate of in vitro incorporation of [3H]uridine into RNA by pancreas slices derived from two groups of rats fed either a low-selenium diet or a diet supplemented with 0.25 mg/kg selenium as selenite was examined. The RNA and lipid peroxide contents and glutathione peroxidase activity in homogenates from the pancreas were also determined. After feeding for 12-14 weeks, the rates of [3H]uridine incorporation were significantly higher in the pancreatic tissue from the selenium-supplemented diet group. Concomitantly, an increase in glutathione peroxidase activities and RNA content, and a reduction of lipid peroxides, were also found in the pancreatic tissue of the selenium-supplemented group. The results suggest that selenium supplementation at a level of 0.25 mg/kg selenium could promote RNA synthesis with an increase in glutathione peroxidase activity and a decrease of lipid peroxides.
Chen
F S
FS
Department of Biochemistry, Xi'an Medical University, Shaanxi, PRC.
Zhao
J Y
JY
Xu
G L
GL
Jia
X A
XA
D000818:Animals; D004247:DNA / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000494:pharmacology*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D008054:Lipid Peroxides; D014316:Tritium; D012313:RNA; D004247:DNA; D005979:Glutathione Peroxidase; D012643:Selenium; D014529:Uridine