Charybdotoxin blocks cation-channels in the vacuolar membrane of suspension cells of Chenopodium rubrum L.
Using the patch-clamp technique, we studied the action of charybdotoxin which blocks Ca(2+)-activated large-conductance K+ channels in animal tissue on the slow-activating (SV), Ca(2+)-activated cation channel in the vacuolar membrane of suspension-cells of Chenopodium rubrum L. The toxin reversibly reduced the vacuolar current with EC50 approximately 20 nM suggesting structural similarities between ion channels in animal and plant membranes.
Weiser
T
T
Botanisches Institut der Justus-Liebig-Universität, Giessen, F.R.G.
Bentrup
F W
FW
D002412:Cations; D018999:Charybdotoxin; D004553:Electric Conductivity; D007425:Intracellular Membranes / Q000378:metabolism*; D007473:Ion Channels / Q000187:drug effects* / Q000378:metabolism; D010944:Plants / Q000187:drug effects / Q000378:metabolism; D012604:Scorpion Venoms / Q000494:pharmacology*; D014617:Vacuoles / Q000187:drug effects* / Q000378:metabolism
D002412:Cations; D007473:Ion Channels; D012604:Scorpion Venoms; D018999:Charybdotoxin