Comparison of alpha 1 adrenoceptors in the prostate capsule of men with symptomatic and asymptomatic benign prostatic hyperplasia.
The objective of this study was to compare the binding and functional properties of alpha 1 adrenoceptors in prostate capsules obtained from men with symptomatic and asymptomatic benign prostatic hyperplasia (BPH) undergoing simple retropubic prostatectomy and cystoprostatectomy respectively. Saturation experiments using 125I-Heat demonstrated that the density and binding affinity of alpha 1 adrenoceptors in the prostate capsules obtained from men with symptomatic and asymptomatic BPH were similar. Non-cumulative dose response experiments using phenylephrine demonstrated that the magnitude of the contractile response to phenylephrine was 4-fold greater in the prostate capsules from men with symptomatic BPH than from those with asymptomatic BPH. The EC50 of phenylephrine in the prostate capsules of men with symptomatic and asymptomatic BPH was similar. A correlation between alpha 1 adrenoceptor density and phenylephrine Emax was not observed, implying that either alpha 1 adrenoceptors are not localised exclusively to the prostate smooth muscle or that spare alpha 1 adrenoceptors exist. This study suggests that the neuropharmacological properties of the prostate capsule may play a significant role in the development of infravesical obstruction in the ageing male population.
Lepor
H
H
Division of Urologic Surgery, Jewish Hospital of St Louis.
Gup
D I
DI
Baumann
M
M
Shapiro
E
E
D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D006801:Humans; D007457:Iodine Radioisotopes / Q000378:metabolism; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D011468:Prostatectomy / Q000379:methods; D011470:Prostatic Hyperplasia / Q000378:metabolism* / Q000601:surgery; D011869:Radioligand Assay / Q000379:methods; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*
D007457:Iodine Radioisotopes; D011942:Receptors, Adrenergic, alpha; D010656:Phenylephrine