Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6.
The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme (E2) that is required for DNA repair, damage-induced mutagenesis, and sporulation. We have cloned the two human RAD6 homologs, designated HHR6A and HHR6B. The two 152-amino acid human proteins share 95% sequence identity with each other and approximately 70% and approximately 85% overall identity with the homologs from yeasts (S. cerevisiae and Schizosaccharomyces pombe) and Drosophila melanogaster, respectively. Neither of the human RAD6 homologs possess the acidic C-terminal sequence present in the S. cerevisiae RAD6 protein. Genetic complementation experiments reveal that HHR6A as well as HHR6B can carry out the DNA repair and mutagenesis functions of RAD6 in S. cerevisiae rad6 delta mutants.
Koken
M H
MH
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Reynolds
P
P
Jaspers-Dekker
I
I
Prakash
L
L
Prakash
S
S
Bootsma
D
D
Hoeijmakers
J H
JH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D004260:DNA Repair / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D015723:Gene Library; D005800:Genes, Fungal; D006801:Humans; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D044763:Ubiquitin-Conjugating Enzymes
D015342:DNA Probes; D005656:Fungal Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D011061:Poly A; D012313:RNA; C471606:RAD6 protein, S cerevisiae; C471545:UBE2A protein, human; C471546:UBE2B protein, human; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases