Ber-MAC3: new monoclonal antibody that defines human monocyte/macrophage differentiation antigen.
A new monoclonal antibody Ber-MAC3 is reported. It recognises a formol sensitive epitope of a not yet clustered monocyte/macrophage specific 140 kilodalton glycoprotein that is expressed on the cell surface and in the cytoplasm. In 30 cases of acute and chronic leukaemia, Ber-MAC3 staining was restricted to 15 myeloid leukaemias of M4 and M5 types. The tumour cells of two cases of true histiocytic malignancies were Ber-MAC3 positive, whereas those of all 280 malignancies of lymphocytic origin were negative. The latter included 52 cases of Hodgkin's disease and 41 cases of Ki-1 positive anaplastic large cell lymphomas which had previously been classified as true histiocytic lymphomas. Ber-MAC3 therefore seems to be of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and seems to be suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin.
Backé
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E
Institut für Pathologie, Klinikum Steglitz, Berlin, Germany.
Schwarting
R
R
Gerdes
J
J
Ernst
M
M
Stein
H
H
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D015620:Histiocytic Disorders, Malignant / Q000175:diagnosis; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D008970:Molecular Weight; D009000:Monocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000939:Epitopes; D008562:Membrane Glycoproteins; C036763:monocyte-macrophage differentiation antigen