Identification of platelet antigens by immunoprecipitation of unlabelled platelet glycoproteins.
A time-saving and sensitive method for the identification of antigens on unlabelled platelet glycoproteins (GP) based on immunoprecipitation has been developed. Platelet solubilisates were incubated with antibodies to form GP-antibody complexes that were precipitated with Protein G Sepharose 4 Fast Flow (PGS). PGS-bound material was eluted, separated by SDS-PAGE under reducing conditions and visualized by silver staining. The method was designed as an alternative to radioimmunoprecipitation for laboratories not equipped to work with radioactive substances. It is proposed as a complementary procedure for the characterization of platelet GP antigens by murine monoclonal antibodies and human alloantibodies.
Stenzinger
W
W
Department of Internal Medicine, University of Münster, Federal Republic of Germany.
Kehrel
B
B
van de Loo
J
J
D000143:Acids; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007519:Isoantigens / Q000097:blood*; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D011233:Precipitin Tests; D012834:Silver; D012995:Solubility; D013194:Staining and Labeling
D000143:Acids; D000911:Antibodies, Monoclonal; D007519:Isoantigens; D010980:Platelet Membrane Glycoproteins; D012834:Silver