Mapping of linear B-cell epitopes of hepatitis B surface antigen.
Eight monoclonal antibodies directed against the surface protein of hepatitis B virus (HBV) were tested using an epitope-mapping system (Pepscan) for characterizing antigenic domains. Four different amino acid sequences corresponding to linear epitopes were identified: one in pre-S1 corresponding to the sequence 29-36, two in pre-S2 corresponding to overlapping sequences 134-141 and 137-144, and one in the S region of the protein corresponding to the amino acid sequence 117-126.
Coursaget
P
P
Institut de Virologie de Tours and Laboratoire de Microbiologie-Immunologie, Faculté de Pharmacie, France.
Lesage
G
G
Le Cann
P
P
Mayelo
V
V
Bourdil
C
C
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000276:immunology
D000939:Epitopes; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen