Multiple apomucin translation products from human respiratory mucosa mRNA.
Poly(A)-rich RNA was purified from a pool of five human tracheobronchial mucosa. After in vitro translation in a reticulocyte lysate and immunoprecipitation of the translated products, using either a polyclonal antiserum or a monoclonal antibody to deglycosylated respiratory mucin peptides, the products were characterized by SDS/PAGE. The respiratory mucin precursors migrated as a very large smear from almost the top of the resolving polyacrylamide gel to an area corresponding to a molecular mass of about 100 kDa. After hybridization with mucin cDNA probe TH 29 described by Crepin et al. [Crepin, M., Porchet, N., Aubert, J. P. & Degand, P. (1990) Biorheology 27, 471-484] respiratory mucin mRNAs also appeared polydisperse. Although degradation or incomplete translation of high-molecular-mass mRNA cannot be entirely ruled out, these results suggest that human respiratory apomucins consist of a family of peptides which share some common epitopes. This possibility is in agreement with (a) the diversity of mucin precursors observed previously with pulse/chase experiments performed with explants of human respiratory mucosa and (b) the polydispersity of secreted respiratory mucins observed by electron microscopy.
Perini
J M
JM
Unité No 16 de l'Institut National de la Santé et de la Recherche Médicale and Groupement de Recherche No 139, Centre National de la Recherche Scientifique, Lille, France.
Vandamme-Cubadda
N
N
Aubert
J P
JP
Porchet
N
N
Mazzuca
M
M
Lamblin
G
G
Herscovics
A
A
Roussel
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005752:Gastric Mucins; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009077:Mucins / Q000235:genetics; D009092:Mucous Membrane / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D012137:Respiratory System / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D015342:DNA Probes; D000939:Epitopes; D005752:Gastric Mucins; D009077:Mucins; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; C053366:apomucin; D004247:DNA