Keratinization is associated with the expression of a new protein related to the desmosomal cadherins DGII/III.
Amino acid sequencing of a 48/46 kDa glycoprotein from human plantar callus, recognised by antisera raised against the desmosomal cadherins DGII/III, has revealed N-terminal homology to the DNA-derived sequence of human and bovine DGII/III. However, a tryptic fragment has homology only with a bovine clone. We propose that there are two classes of DGII/III-like molecule, that represented by the bovine cDNA clone and the 48/46 kDa protein, a monoclonal antibody against which stains mainly the suprabasal layers of human epidermis, and that represented by the human cDNA clone, identified by a monoclonal antibody which stains uniformly the living layers of the epidermis.
King
I A
IA
Dermatology Research Group, Clinical Research Centre, Harrow, Middlesex, UK.
Magee
A I
AI
Rees
D A
DA
Buxton
R S
RS
D000595:Amino Acid Sequence; D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002417:Cattle; D003896:Desmosomes / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007633:Keratins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D016415:Sequence Alignment
D015820:Cadherins; D010446:Peptide Fragments; D007633:Keratins