Stimulation of fatty acid oxidation in myocytes by phosphodiesterase inhibitors and adenosine analogues.
The effect of various phosphodiesterase inhibitors, and adenosine analogues on palmitate oxidation, were studied in isolated rat myocytes. Enoximone, milrinone, and dipyridamole, at a concentration of 250 microM, stimulated palmitate oxidation by 78%, 40%, and 43%, respectively. The specific A1-agonist, N6-cyclopentyladenosine, increased palmitate oxidation by 56%, at a concentration of 250 microM. Moreover, the nucleoside transport inhibitor, S-(P-Nitrobenzyl-)6-thioinosine, increased palmitate oxidation by 40%, at a concentration of 100 microM. These data suggest that the stimulation of palmitate oxidation by enoximone and adenosine analogues may be mediated via the inhibition of the uptake and/or the oxidation of glucose in myocytes.
Abdel-Aleem
S
S
Glaxo Inc., Department of Pharmacology, Research Triangle Park, NC 27709.
Badr
M
M
Frangakis
C
C
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000676:Amrinone / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D004176:Dipyridamole / Q000494:pharmacology; D017335:Enoximone; D006321:Heart / Q000187:drug effects; D007093:Imidazoles / Q000494:pharmacology; D020105:Milrinone; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D011688:Purinones / Q000494:pharmacology; D011724:Pyridazines / Q000494:pharmacology; D011728:Pyridones / Q000494:pharmacology; D051381:Rats
D007093:Imidazoles; D010169:Palmitic Acids; D010726:Phosphodiesterase Inhibitors; D011688:Purinones; D011724:Pyridazines; D011728:Pyridones; D019308:Palmitic Acid; D004176:Dipyridamole; C042528:4,5-dihydro-6-(4-(imidazol-1-yl)phenyl)-5-methyl-3(2H)-pyridazinone; D017335:Enoximone; C011145:zaprinast; D020105:Milrinone; D000676:Amrinone; D000241:Adenosine; D015056:1-Methyl-3-isobutylxanthine