title: A second neutralizing epitope of B19 parvovirus implicates the spike region in the immune response.
abstract: We used 18 monoclonal antibodies against B19 parvovirus to identify neutralizing epitopes on the viral capsid. Of the 18 antibodies, 9 had in vitro neutralizing activity in a bone marrow colony culture assay. The overlapping polypeptide fragments spanning the B19 structural proteins were produced in a pMAL-c Escherichia coli expression system and used to investigate the binding sites of the neutralizing antibodies. One of the nine neutralizing antibodies reacted with both VP1 and VP2 capsid proteins and a single polypeptide fragment on an immunoblot, identifying a linear neutralizing epitope between amino acids 57 and 77 of the VP2 capsid protein. Eight of nine neutralizing antibodies failed to react with either of the capsid proteins or any polypeptide fragments, despite reactivities with intact virions in a radioimmunoassay, suggesting that additional conformationally dependent neutralizing epitopes exist.
authors.affiliation: Cell Biology Section, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892.
authors.forename: K
authors.initials: K
authors.lastname: Yoshimoto
authors.forename: S
authors.initials: S
authors.lastname: Rosenfeld
authors.forename: N
authors.initials: N
authors.lastname: Frickhofen
authors.forename: D
authors.initials: D
authors.lastname: Kennedy
authors.forename: R
authors.initials: R
authors.lastname: Hills
authors.forename: S
authors.initials: S
authors.lastname: Kajigaya
authors.forename: N S
authors.initials: NS
authors.lastname: Young
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D002352:Carrier Proteins / Q000235:genetics; D003114:Colony-Forming Units Assay; D000939:Epitopes / Q000032:analysis*; D029968:Escherichia coli Proteins; D005814:Genes, Viral; D007074:Immunoglobulin G / Q000145:classification / Q000276:immunology; D008320:Maltose / Q000378:metabolism; D058619:Maltose-Binding Proteins; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D009500:Neutralization Tests; D010321:Parvoviridae / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018528:ATP-Binding Cassette Transporters; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; D002352:Carrier Proteins; D000939:Epitopes; D029968:Escherichia coli Proteins; D007074:Immunoglobulin G; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C061332:VP6 protein, Rotavirus; C461599:maltose transport system, E coli; D008320:Maltose