title: Generation of anti-peptide and anti-protein sera. Effect of peptide presentation on immunogenicity.
abstract: The technique of Fmoc chemistry has been applied successfully to the synthesis of branched peptides. The immunogenicity of branched peptides has been compared quantitatively with those of protein-conjugated and resin-linked peptides. Six different peptide sequences were used to immunise rabbits and both antipeptide and anti-protein titres were determined for each serum. The data show that the titres of sera from rabbits immunised with branched peptides were higher than those of rabbits immunised with protein-conjugated peptides which in turn were higher than those immunised with resin-linked peptides. The effect was demonstrated with two strains of rabbits.
authors.affiliation: Institute of Virology, Glasgow, Scotland, U.K.
authors.forename: G W
authors.initials: GW
authors.lastname: McLean
authors.forename: A M
authors.initials: AM
authors.lastname: Owsianka
authors.forename: J H
authors.initials: JH
authors.lastname: Subak-Sharpe
authors.forename: H S
authors.initials: HS
authors.lastname: Marsden
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000276:immunology; D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005449:Fluorenes; D007114:Immunization; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits; D018139:Simplexvirus / Q000276:immunology; D013329:Structure-Activity Relationship; D014764:Viral Proteins / Q000138:chemical synthesis / Q000276:immunology
chemical_list: D000596:Amino Acids; D000939:Epitopes; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010446:Peptide Fragments; D010455:Peptides; D014764:Viral Proteins