Report of studies on monoclonal anti-IgG antibodies.
Eleven monoclonal anti-IgG antibodies were evaluated by ten laboratories for possible AHG reagent use by the ISBT/ICSH protocol. Three of the anti-IgGs were selected by tests with weak Fya sensitized cells as this represents the best screening test. One of the IgM class anti-IgGs (205) equalled ISBT/ICSH reference reagents (R3P and RIIIM) against weak anti-D, -K and Fya sensitized cells and it reacted with all four subclasses of IgG. Thus, subject to satisfactory stability and extended trials with a wide range of weak antibodies, monoclonal anti-IgGs for AHG reagent use are now a possible alternative to conventional rabbit anti-IgG.
Voak
D
D
Regional Transfusion and Immuno-Haematology Centre, Cambridge, U.K.
Nilsson
U
U
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D000915:Antibody Affinity; D000939:Epitopes / Q000032:analysis; D004912:Erythrocytes / Q000276:immunology; D005189:False Positive Reactions; D006801:Humans; D007074:Immunoglobulin G
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G