Direct detection of yeast mutants with reduced viability on plates by erythrosine B staining.
In this paper we report a rapid method to screen yeast mutants exhibiting reduced viability directly on plates. This method avoids the need for replica plating and is based on the addition of the vital dye erythrosine B in nutrient medium. After 2 or 3 days of culture, colonies containing a large proportion of dead cells show a pink or a dark pink color whereas normal colonies are practically white.
Bonneu
M
M
Laboratoire de Génétique, Université de Bordeaux II, Talence, France.
Crouzet
M
M
Urdaci
M
M
Aigle
M
M
D015169:Colony Count, Microbial / Q000379:methods; D004923:Erythrosine; D008828:Microbiological Techniques; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D013194:Staining and Labeling
D004923:Erythrosine