title: Surrogate markers are not useful for identification of HCV carriers in chronic hemodialysis patients.
abstract: Several diagnostic hepatitis C assays have been developed for the detection of antibodies to different antigens of the virus. This virus is the major cause of non-A, non-B hepatitis. Seventy-nine patients undergoing chronic hemodialysis and/or hemofiltration were tested for the presence of anti-HCV antibodies (anti-C-100-3 antibodies and anti-core antibodies), anti-hepatitis B core antibodies (anti-HBc), and aminotransferases (ALT). Seven patients were positive by one or more of the anti-HCV enzyme linked immunoassays (EIAs), while HCV-RNA was detectable in only four patients. These four patients had at least one, but not necessarily the same, positive anti-HCV EIA. HCV-RNA was not detected in patients who had no antibodies as determined by all six anti-HCV EIAs. All patients with a marker for HCV infection had persistent normal levels of transaminases. Three patients had elevated ALT values without a marker for HCV infection and suffered from hepatitis B virus infection. Anti-HBc was detected in 27/72 patients without any marker and in four patients with a marker of HCV infection. However, HCV-RNA was detectable in only one of these four anti-HBc positive patients. It is concluded that surrogate markers (anti-HBc and serum transaminases) are not useful for identification of HCV carriers in chronic hemodialysis patients.
authors.affiliation: Department of Internal Medicine, University Hospital Gasthuisberg, Leuven, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Willems
authors.forename: G
authors.initials: G
authors.lastname: de Jong
authors.forename: H
authors.initials: H
authors.lastname: Moshage
authors.forename: L
authors.initials: L
authors.lastname: Verresen
authors.forename: P
authors.initials: P
authors.lastname: Goubau
authors.forename: J
authors.initials: J
authors.lastname: Desmyter
authors.forename: S H
authors.initials: SH
authors.lastname: Yap
mesh_terms: D015415:Biomarkers; D002353:Carrier State / Q000175:diagnosis*; D005069:Evaluation Studies as Topic; D006440:Hemofiltration / Q000009:adverse effects*; D006508:Hepatitis Antibodies / Q000097:blood; D006512:Hepatitis B Core Antigens / Q000276:immunology; D006526:Hepatitis C / Q000175:diagnosis*; D018937:Hepatitis C Antibodies; D006801:Humans; D012367:RNA, Viral / Q000097:blood; D006435:Renal Dialysis / Q000009:adverse effects*; D000637:Transaminases / Q000097:blood
chemical_list: D015415:Biomarkers; D006508:Hepatitis Antibodies; D006512:Hepatitis B Core Antigens; D018937:Hepatitis C Antibodies; D012367:RNA, Viral; D000637:Transaminases