The 16S ribosomal RNA of Mycobacterium leprae contains a unique sequence which can be used for identification by the polymerase chain reaction.
Nucleotide sequence data for bacterial 16S ribosomal RNA was used to identify oligodeoxyribonucleotide primers suitable for probing the rRNA gene of mycobacteria and related organisms, with the polymerase chain reaction. The method enabled us to distinguish mycobacteria from other closely related genera, and to differentiate between slow- and fast-growing mycobacteria. Mycobacterium leprae fell within the slow-growing group of mycobacteria but there are significant differences between the sequence of the M. leprae 16S rRNA gene and that of other slow-growing mycobacteria. These differences were used to devise a rapid, non-radioactive method for detecting M. leprae in infected tissue.
Cox
R A
RA
National Institute for Medical Research, London.
Kempsell
K
K
Fairclough
L
L
Colston
M J
MJ
D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D007918:Leprosy / Q000235:genetics / Q000382:microbiology; D051379:Mice; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S