Differential regulation of the two mRNA species of the rodent negative acute phase protein alpha 1-inhibitor 3.
Screening of two rat liver cDNA libraries, one of which was constructed using an alpha 1-inhibitor 3 (alpha 1-13) specific primer, yielded overlapping cDNA clones which correspond to the full length cDNA for alpha 1-13 mRNA. On the basis of sequence microheterogeneity existing throughout the cDNA sequence we identified two alpha 1-13 mRNA species whose sequences are so grossly different in their bait regions that the amino acid homology therein is only 30%. Using oligonucleotide probes derived from their respective bait regions we investigated the regulation of the two alpha 1 I3 mRNA species and demonstrated that only one of them, alpha 1-I3 variant I, is regulated pretranslationally following experimentally induced inflammation.
Regler
R
R
Institut für Mikrobiologie und Biochemie der Universität Erlangen-Nürnberg, Germany.
Sickinger
S
S
Schweizer
M
M
D000209:Acute-Phase Proteins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D008297:Male; D008969:Molecular Sequence Data; D011480:Protease Inhibitors; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping
D000209:Acute-Phase Proteins; D011480:Protease Inhibitors; D012333:RNA, Messenger; C017894:alpha 1-inhibitor 3; D004247:DNA