title: Alpha 2-macroglobulin synthesis in interleukin-6-stimulated human neuronal (SH-SY5Y neuroblastoma) cells. Potential significance for the processing of Alzheimer beta-amyloid precursor protein.
abstract: Cultured human neuronal (SH-SY5Y neuroblastoma) cells synthesize and secrete the potent protease inhibitor alpha 2-macroglobulin (a2M) upon stimulation with interleukin-6 (IL-6) indicating that alpha 2-macroglobulin behaves as an acute-phase protein in the human central nervous system. Exogenous addition of a2M to the cultured neuronal cells resulted in only a slight inhibition of Alzheimer beta A4-amyloid precursor protein (APP) synthesis, but markedly inhibited its secretion pointing to the possibility that a2M may affect the proteolytic APP processing. Evidence is provided that IL-6 and a2M are involved in Alzheimer's disease pathogenesis.
authors.affiliation: Psychiatrische Universitätsklinik, Universität Freiburg, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Ganter
authors.forename: S
authors.initials: S
authors.lastname: Strauss
authors.forename: U
authors.initials: U
authors.lastname: Jonas
authors.forename: A
authors.initials: A
authors.lastname: Weidemann
authors.forename: K
authors.initials: K
authors.lastname: Beyreuther
authors.forename: B
authors.initials: B
authors.lastname: Volk
authors.forename: M
authors.initials: M
authors.lastname: Berger
authors.forename: J
authors.initials: J
authors.lastname: Bauer
mesh_terms: D000544:Alzheimer Disease / Q000209:etiology / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000378:metabolism; D016564:Amyloid beta-Protein Precursor; D006801:Humans; D015850:Interleukin-6 / Q000502:physiology*; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism*; D011233:Precipitin Tests; D011480:Protease Inhibitors; D011498:Protein Precursors / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000511:alpha-Macroglobulins / Q000096:biosynthesis*
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D015850:Interleukin-6; D011480:Protease Inhibitors; D011498:Protein Precursors; D000511:alpha-Macroglobulins