Metabolic and structural effects of HIV infection in human peripheral blood mononuclear cells can be monitored with 1H NMR spectroscopy.
Infection of human peripheral blood lymphocytes by human immunodeficiency virus type 1 (HIV-1) was investigated by means of 1H nuclear magnetic resonance spectroscopy, taking advantage of the presence of signals from fluid lipid domains in the membrane of stimulated lymphocytes. A transient decrease of the lipid methylene signal intensity was observed at the time of HIV internalization, monitoring a general rearrangement of membrane structure associated with virus entry. A similar effect was also observed a few days after infection, when HIV particles are released by infected cells as demonstrated by high reverse transcriptase activity in cell supernatant. Signals arising from choline-based metabolites were also affected by HIV infection, indicating a possible slowing down of phospholipid synthesis.
Federico
M
M
Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.
Maggiorella
M T
MT
Sulli
N
N
Guidoni
L
L
Luciani
A M
AM
Rosi
A
A
Viti
V
V
Rossi
G B
GB
Verani
P
P
D002474:Cell-Free System; D002478:Cells, Cultured; D015658:HIV Infections / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D054303:HIV Reverse Transcriptase; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008213:Lymphocyte Activation; D009682:Magnetic Resonance Spectroscopy; D015166:Monitoring, Immunologic; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D013997:Time Factors
D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase