title: [Diagnostic use of neutrophil segments negative for peroxidase, chloroacetate esterase and sudanophilia in peripheral blood in leukemia].
abstract: The paper contains the results of three cytochemical reactions used for detection of myeloid differentiation (peroxidase, sudanophilia, chloroacetate esterase) in neutrophil segments of the peripheral blood stream in 107 patients with acute myeloid or lymphatic leukaemia. Enzymatically deficient segments were detected in 23 (34.8%) patients with acute myeloid leukaemia. They were not found in any patients lymphatic leukaemia not in healthy subjects. The concurrent deficit of all three reactions was found in 69% of the cases with defective neutrophil segments. However, also isolated affection of any of these reactions was found. In acute leukaemias, not differentiated from the cytochemical and immunophenotypical aspect, we may consider simple evidence of the presence of the mentioned abnormal neutrophil segments in peripheral blood as a highly probable sign of myeloid differentiation of acute leukaemia.
authors.affiliation: I. interní klinika Fakultní nemocnice KUNZ, Plzen.
authors.forename: P
authors.initials: P
authors.lastname: Skopek
authors.forename: V
authors.initials: V
authors.lastname: Koza
mesh_terms: D000208:Acute Disease; D001391:Azo Compounds; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D004796:Clinical Enzyme Tests; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood / Q000175:diagnosis*; D009281:Naphthalenes; D009504:Neutrophils / Q000201:enzymology* / Q000378:metabolism; D009195:Peroxidase / Q000097:blood*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000175:diagnosis*; D013194:Staining and Labeling
chemical_list: D001391:Azo Compounds; D009281:Naphthalenes; C016118:Sudan Black B; D009195:Peroxidase; D002265:Carboxylic Ester Hydrolases; C019873:chloroacetate esterase
vernacular_title: Diagnostické vyuzití neutrofilních segmentů s negativitou peroxidázy, chloracetátesterázy a sudanofilie v periferní krvi leukemiků.