Bleomycin-detectable iron in brain tissue.
The normal brain contains regions with high concentrations of iron, part of which appears to be in a low molecular mass chelatable form. Iron complexes with a molecular mass of below 10,000, were measured in ultrafiltrates of homogenized gerbil brains using the bleomycin assay, and were found to average 20.5 +/- 3.5 microM (n = 8). As expected, no bleomycin detectable iron was found in the plasma of these animals. No obvious difference in the tissue levels of bleomycin-detectable iron was recorded following ischaemia and reperfusion. This is probably due to the already abundant presence of iron in the brain and the likely release of iron from protected sites due to structural damage inherent in the preparative procedures used.
Gutteridge
J M
JM
Molecular Toxicology Research Group, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Cao
W
W
Chevion
M
M
D000818:Animals; D001761:Bleomycin; D001921:Brain / Q000378:metabolism*; D005609:Free Radicals; D005849:Gerbillinae; D007202:Indicators and Reagents; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000378:metabolism; D008297:Male
D005609:Free Radicals; D007202:Indicators and Reagents; D007502:Iron Chelating Agents; D001761:Bleomycin; D007501:Iron