Absence of O6-alkylguanine-DNA alkyltransferase induction in chick embryo liver and brain following X-irradiation or treatment with bleomycin.
1. The presence of O6-alkylguanine-DNA alkyltransferase (AT) in liver and brain of chick embryos, chicks and hens was demonstrated. An induction of AT activity has only been found in the liver of chicks and hens 48 hr after X-irradiation (5, 10 or 12 Gy). 2. The administration of methylmethanesulphonate to the chick embryo resulted 3-24 hr later in strong inhibition of AT activity accompanied by DNA alkylation. Under the same conditions, X-irradiation, dimethylnitrosamine and bleomycin exhibited no effect. 3. The results are compared with those obtained in mouse, rat and human foetal tissues.
Stammberger
I
I
Institute of Pharmacology, Toxicology and Pharmacy, Faculty of Veterinary Medicine, University of Munich, FRG.
Tempel
K
K
D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D001921:Brain / Q000201:enzymology*; D002642:Chick Embryo; D004790:Enzyme Induction / Q000187:drug effects / Q000528:radiation effects; D008099:Liver / Q000201:enzymology*; D008780:Methyltransferases / Q000096:biosynthesis*; D019853:O(6)-Methylguanine-DNA Methyltransferase
D001761:Bleomycin; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase