A stable flank of unstable lymphotropic papovavirus integration sites is associated with a cellular S1 nuclease-sensitive sequence.
Integration of viral DNA sequences is associated with stable transformation by viruses of the polyomavirus genus. In LPV-HE cells, a hamster embryonal cell line transformed by the African green monkey lymphotropic papovavirus (LPV), viral integration is unstable during tissue culture passage, leading to subclones with rearrangements of the original integration locus. Three of four viral integrations that were molecularly cloned together with flanking cellular sequences are identical in one junction but are different from each other at the other flank. The "stable" flank which is already present in early passage cells contains an S1 nuclease-sensitive sequence located approximately 60 bp outside of the viral-cellular junction. The presence of this site may contribute to the stability of the flanking region.
Mühlbach
P
P
Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.
Pawlita
M
M
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D004279:DNA, Viral / Q000235:genetics; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics*; D027382:Polyomaviridae; D015183:Restriction Mapping; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D016662:Virus Integration
D004279:DNA, Viral; D015719:Single-Strand Specific DNA and RNA Endonucleases