Identification and characterization of a rat protein (p 105) auto-antigenic in rats bearing a progressive syngeneic colon carcinoma.
Sera from BDIX rats inoculated with 2 tumor clones derived from a single syngeneic colon carcinoma were assayed by Western blotting for the presence of antibodies against the grafted tumor. The PROb clone is progressive and produces metastases. We observed that rats bearing this tumor developed antibodies against an unglycosylated water-soluble protein of 105 kDa. The magnitude of this humoral response, as assessed by the intensity of the signal on immunoblots, was inversely correlated with survival of the rats. Furthermore, rats inoculated with the REGb clone, which is immunologically rejected, never developed detectable antibodies against the tumor. Antisera from rats injected with PROb tumor detected p105 antigen in cellular extracts from the REGb clone and from a series of rat and human cell lines. This protein was also detected in variable amounts in some normal adult and fetal tissues. Treatment of PROb or REGb cells by either interferon-gamma or heat shock did not significantly alter the expression of the p105 auto-antigen.
Blottière
H M
HM
INSERM CJF 90.11, Faculty of Medicine, Nantes, France.
Menoret
A
A
Burg
C
C
Douillard
J Y
JY
Le Pendu
J
J
D000230:Adenocarcinoma / Q000097:blood / Q000276:immunology*; D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis; D000917:Antibody Formation / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis*; D015153:Blotting, Western; D003110:Colonic Neoplasms / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006360:Heat-Shock Proteins / Q000032:analysis; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology; D009368:Neoplasm Transplantation; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D006360:Heat-Shock Proteins; D007371:Interferon-gamma