Halogenated alcohols as solvents for proteins: FTIR spectroscopic studies.
Fourier transform infrared (FTIR) spectroscopy has been applied to investigate the secondary structure of proteins and polypeptides in halogenated alcohols. Each alcohol studied was able, as a pure liquid, to induce conversion of the beta-sheet protein concanavalin A into a predominantly alpha-helical configuration. In 2H2O/alcohol mixtures, helicogenisis was also apparent, decreasing in the order dichloroethanol greater than bromoethanol greater than trifluoroethanol greater than chloroethanol greater than fluoroethanol. At concentrations below those found to be helicogenic, disruption of the protein secondary structure by the alcohols resulted in pronounced aggregation. At concentrations insufficient to cause noticeable disruptions of the secondary structure at room temperature, the thermal stability of the protein was greatly reduced. We suggest the helicogenic effect exhibited by halogenated alcohols to be related to a combination of a relatively low dielectric constant and a high dipole moment, the latter causing disruption of the internal hydrogen bond networks and the former causing refolding to a helical configuration. The results presented here highlight the risk of using halogenated alcohols, both as solvents for proteins and as a test of the intrinsic capacity of proteins and peptides to adopt helical secondary structures.
Jackson
M
M
Steacie Institute for Molecular Sciences, National Research Council Canada, Ottawa, Ontario.
Mantsch
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D000438:Alcohols / Q000737:chemistry*; D000818:Animals; D003208:Concanavalin A / Q000737:chemistry*; D004553:Electric Conductivity; D005583:Fourier Analysis; D006219:Halogens / Q000737:chemistry*; D006736:Horses; D009206:Myocardium / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry*; D012997:Solvents; D013055:Spectrophotometry, Infrared
D000438:Alcohols; D006219:Halogens; D009211:Myoglobin; D012997:Solvents; D003208:Concanavalin A