Detection of the membrane inhibitor of reactive lysis (CD59) in diseased neurons of Alzheimer brain.
The membrane inhibitor of reactive lysis (MIRL) protects host cells from complement-mediated lysis. It was detected immunohistochemically in tangled neurons and dystrophic neurites of Alzheimer disease (AD) tissue in a pattern highly similar to that observed for the membrane attack complex of complement, C5b-9. MIRL was also detected in cultured IMR-32 neuroblastoma cells. The mRNA for MIRL was detected in RNA extracts of both AD and normal brain. These data provide the first evidence of brain neuronal expression of MIRL and its upregulation in neurons exposed to complement attack. They are consistent with the previously advanced hypothesis that complement-mediated neuronal injury may play a role in AD.
McGeer
P L
PL
Kinsmen Laboratory of Neurological Research, Department of Psychiatry, University of British Columbia, Vancouver, Canada.
Walker
D G
DG
Akiyama
H
H
Kawamata
T
T
Guan
A L
AL
Parker
C J
CJ
Okada
N
N
McGeer
E G
EG
D000544:Alzheimer Disease / Q000378:metabolism*; D000595:Amino Acid Sequence; D000943:Antigens, Differentiation / Q000032:analysis* / Q000235:genetics; D001921:Brain / Q000378:metabolism*; D018957:CD59 Antigens; D015938:Complement Membrane Attack Complex / Q000032:analysis; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger
D000943:Antigens, Differentiation; D018957:CD59 Antigens; D015938:Complement Membrane Attack Complex; D008562:Membrane Glycoproteins; D012333:RNA, Messenger