Time-course variations induced by pargyline on the 5-hydroxyindole compounds measured in the nucleus raphe dorsalis and in blood: a voltammetric and HPLC approach in the rat.
Ninety min after pargyline (Pargy, 75 mg/kg) injection, the +300 mV voltammetric signal, measured in the nucleus raphe dorsalis (nRD) of freely moving rats, disappeared completely. This effect was also followed (2-7 h post-injection) by the reappearance of a peak (post-Pargy-peak) at the same +300 mV potential. The height of this post-Pargy signal was still decreased by Pargy (30 or 75 mg/kg). Endogenous 5-hydroxyindoleacetic acid (5-HIAA) contents, measured with HPLC in the nRD of Pargy-treated rats, exhibited analogous variations. Inhibition of monoamine oxidases by Pargy seems thus to be effective in blocking 5-HIAA production over only 2 h. The 15-fold increase of 5-HT endogenous content in the nRD (3 h after injection) was not reflected in the voltammetric extracellular measurements performed 90 min to 7 h after Pargy injection; this increase is suspected to be mainly intracellular.
Houdouin
F
F
Département de Médecine Expérimentale, INSERM U52-CNRS UA 1195, Université Claude Bernard, Lyons, France.
Cespuglio
R
R
Gharib
A
A
Sarda
N
N
Jouvet
M
M
D000818:Animals; D001545:Benserazide / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006897:Hydroxyindoleacetic Acid / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010293:Pargyline / Q000494:pharmacology*; D011903:Raphe Nuclei / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012701:Serotonin / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D013997:Time Factors
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D001545:Benserazide; D010293:Pargyline