title: Demonstration of acid phosphatase activity in antigenic glycoprotein fractions obtained from the culture filtrate of Pseudomonas pseudomallei.
abstract: Pseudomonas pseudomallei, the causative microorganism of melioidosis, was grown in Mueller-Hinton liquid medium, and glycoprotein fractions were separated from the culture filtrate by ammonium sulfate precipitation, gel-filtration with Sephadex G-75, and column chromatography with DEAE-cellulose. The fractions revealed acid phosphatase activity, and reacted to the sera from melioidosis patient in gel-diffusion precipitation assay.
authors.affiliation: Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
authors.forename: E
authors.initials: E
authors.lastname: Kondo
authors.forename: V
authors.initials: V
authors.lastname: Petkanchanapong
authors.forename: P
authors.initials: P
authors.lastname: Naigowit
authors.forename: T
authors.initials: T
authors.lastname: Kurata
authors.forename: K
authors.initials: K
authors.lastname: Kanai
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000942:Antigens, Bacterial / Q000032:analysis; D016957:Burkholderia pseudomallei / Q000201:enzymology* / Q000276:immunology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005947:Glucose / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008297:Male; D008554:Melioidosis / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D006023:Glycoproteins; D000135:Acid Phosphatase; D005947:Glucose