title: Substance P injections into the ventral tegmentum affect unit activity in mesolimbic terminal regions.
abstract: Microinjections of substance P (SP) into the ventral tegmental area (VTA) increase locomotor activity in rats, and this effect is thought to be produced by activation of the mesolimbic dopamine system. In the present study, firing rates of neurons in areas receiving projections from the mesolimbic dopamine system were recorded during injections of SP (3 microgram in 0.5 microliters saline) into the VTA of rats anesthetized with chloral hydrate. Significant changes in firing rates were observed in 84% of the units recorded in nucleus accumbens and olfactory tubercle. There were mostly decreases in nucleus accumbens (NAC, 21 of 25 units affected by SP) and mostly increases in olfactory tubercle (OT, 13 of 18 units affected by SP). In contrast, neither saline injections into VTA nor SP injections 2 mm dorsal to VTA had any effect on NAC or OT neurons. Haloperidol (0.5 mg/kg IV) blocked the effects of SP, suggesting that effects were mediated, at least in part, by the mesolimbic dopamine system. Results indicated that activation of dopaminergic neurons by SP injections into VTA can produce changes in the activity of neurons in NAC and OT, areas which receive mesolimbic dopaminergic projections.
authors.affiliation: Department of Psychiatry, Emory University School of Medicine, Atlanta 30306.
authors.forename: C H
authors.initials: CH
authors.lastname: West
authors.forename: R P
authors.initials: RP
authors.lastname: Michael
mesh_terms: D000818:Animals; D004594:Electrophysiology; D006220:Haloperidol / Q000494:pharmacology; D008297:Male; D008845:Microinjections; D009434:Neural Pathways / Q000502:physiology; D009714:Nucleus Accumbens / Q000187:drug effects / Q000502:physiology*; D009830:Olfactory Bulb / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000494:pharmacology*; D013681:Tegmentum Mesencephali / Q000187:drug effects / Q000502:physiology*
chemical_list: D013373:Substance P; D006220:Haloperidol