The fever induced by polyriboinosinic:polyribocytidylic acid is not related to interferon synthesis in the rabbit's hypothalamus.
We previously demonstrated that direct administration of interferon (IFN) or its inducer polyriboinosinic acid:polyribocytidylic acid [poly(I:C)] into the hypothalamus caused dose-dependent fever in rabbits. It was not clear whether the fever induced by intrahypothalamic injection of poly(I:C) was due to stimulation of IFN or prostaglandin E-2 synthesis in the hypothalamic tissues. Therefore, in the current experiments, we used an established model in which rabbit hypothalamic minces or brain cells were incubated in vitro with poly(I:C) to test for the ability of poly(I:C) to stimulate IFN or PGE-2 synthesis. The results showed that poly(I:C) stimulated PGE-2, but not IFN, synthesis in the hypothalamus. Thus, it appears that the fever induced by poly(I:C) is not related to IFN synthesis in the hypothalamus.
Won
S J
SJ
Department of Microbiology, National Cheng Kung University Medical College, Tainan, Taiwan, Republic of China.
Lin
M T
MT
Ko
Y H
YH
Chuang
J
J
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001831:Body Temperature / Q000187:drug effects; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis; D005334:Fever / Q000139:chemically induced / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D007275:Injections, Intravenous; D007372:Interferons / Q000096:biosynthesis* / Q000097:blood; D008297:Male; D011070:Poly I-C; D011817:Rabbits; D011863:Radioimmunoassay
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007372:Interferons; D003513:Cycloheximide; D015232:Dinoprostone; D011070:Poly I-C; D007213:Indomethacin