title: Long-term survival and sprouting in culture by motoneurons isolated from the spinal cord of adult frogs.
abstract: Motoneurons of adult frog spinal cord have been retrogradely labeled with the carbocyanine derivative diI. Spinal cords were then dissociated and the labeled motoneurons partially purified by centrifugation over a bovine serum albumin (BSA) density gradient. The resulting cell suspension was plated on a substrate of innervated muscle extracellular matrix (ECM) to which the motoneurons attached and extended processes. Labeled adult motoneurons survived for more than 4 weeks in a defined medium in the absence of added serum or growth factors. These cultures of adult motoneurons provide a favorable preparation for studying molecular factors that influence process outgrowth and synapse formation.
authors.affiliation: Department of Pharmacology, Biocenter of the University, Basel, Switzerland.
authors.forename: D P
authors.initials: DP
authors.lastname: Kuffler
mesh_terms: D000818:Animals; D001370:Axonal Transport; D002232:Carbocyanines; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005109:Extracellular Matrix; D005456:Fluorescent Dyes; D008264:Macrophages / Q000166:cytology; D008297:Male; D009046:Motor Neurons / Q000166:cytology* / Q000648:ultrastructure; D011894:Rana pipiens; D013116:Spinal Cord / Q000166:cytology*
chemical_list: D002232:Carbocyanines; D003470:Culture Media; D005456:Fluorescent Dyes; C024286:3,3'-dioctadecylindocarbocyanine