[A peptide, containing the universal antigenic determinant of tryptophanyl-tRNA-synthetase].
Among clostripain hydrolysate peptides of beef pancreas tryptophanyl-tRNA synthetase the peptide Ile-Ser-Phe-Pro-Ala-Ile-Asn-Gln-Phe-Ala-Ala-Pro-Ser-Gln-Phe-Ser-Ile-Arg was revealed which contains the continuous antigenic determinant for monoclonal antibody Am1. This antibody specifically cross-reacts with tryptophanyl-tRNA synthetases of procaryotes, eucaryotes and archebacteriae. The synthetic peptide with identical amino acid sequence plus N-terminal Arg residue (S-peptide), being immobilized on enzyme immunoassay (EIA) microtitration plate, also binds with Am1. Am1 affinity constant (M-1) measured by non-competitive EIA was (3.0 +/- 0.3).10(7) for S peptide and (1.4 +/- 0.3).10(9) for the native enzyme. The sequence of immunoreactive peptide adopts with high probability the secondary structure including beta-turn(s) and antiparallel beta-sheet composed of inverted repeats. At the same time, the analysis of circular dichroism spectrum (in the far UV) of the peptide dissolved in water comes closest to 16% beta-turn and only 8% beta-sheet. The binding of Am1 with peptide was not observed in aqueous solution.
Zargarova
T A
TA
Zargarov
A A
AA
Bolotina
I A
IA
Beresten'
S F
SF
Favorova
O O
OO
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D002942:Circular Dichroism; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D014369:Tryptophan-tRNA Ligase / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010455:Peptides; D014369:Tryptophan-tRNA Ligase
Peptid, soderzhashchiĭ universal'nuiu antigennuiu determinantu triptoanil-tRNK-sintetaz.