title: Class 1 heparin binding growth factor promotes the differentiation but not the survival of ciliary neurones in vivo.
abstract: Molecules isolated from target tissues of various neuronal populations have been shown to enable the survival of those innervating neurones. A molecule derived from bovine heart which can support the survival of parasympathetic neurones of the chick embryo ciliary ganglion in vitro has been sequenced and identified as a member of the class 1 heparin binding growth factors (HBGF-1). When injected into developing chick embryos during the period of naturally occurring cell death in the ciliary ganglion, HBGF-1 failed to rescue those neurones which normally die. There was, however, a premature increase in the activity of choline acetyltransferase within the ganglion and a shift to the right in the size distribution of the neurones. We conclude that HBGF-1 may promote the differentiation of some subpopulation of neurones of the ciliary ganglion of the chick embryo but does not act in vivo as a survival molecule.
authors.affiliation: Division of Neuroscience, John Curtin School of Medical Research, Canberra, Australia.
authors.forename: C E
authors.initials: CE
authors.lastname: Hill
authors.forename: D A
authors.initials: DA
authors.lastname: Belford
authors.forename: J
authors.initials: J
authors.lastname: Godovac-Zimmermann
authors.forename: I A
authors.initials: IA
authors.lastname: Hendry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D016220:Fibroblast Growth Factor 1 / Q000502:physiology*; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000502:physiology*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry; D009474:Neurons / Q000502:physiology*; D009479:Neuropeptides / Q000502:physiology*
chemical_list: D009479:Neuropeptides; D016220:Fibroblast Growth Factor 1; D002795:Choline O-Acetyltransferase