title: Isolation and amino terminal sequence of beta-trace, a novel protein from human cerebrospinal fluid.
abstract: beta-Trace, a 23.5 kDa glycoprotein of unknown biological functions, is present in all body fluids tested. It is found in higher concentration in human seminal fluid and cerebrospinal fluid (CSF) than in serum. A one-step procedure for the isolation of beta-trace from pooled CSF is described, by affinity chromatography using a specific antibody made against beta-trace. Amino terminal sequence analysis yields the sequence A P E A Q V S V Q P N F Q Q D K F L G with no homology to known proteins, indicating that beta-trace is a novel CSF protein.
authors.affiliation: Department of Neurology, New York University Medical Center, NY 10016.
authors.forename: A P
authors.initials: AP
authors.lastname: Kuruvilla
authors.forename: G M
authors.initials: GM
authors.lastname: Hochwald
authors.forename: J
authors.initials: J
authors.lastname: Ghiso
authors.forename: E M
authors.initials: EM
authors.lastname: Castaño
authors.forename: M
authors.initials: M
authors.lastname: Pizzolato
authors.forename: B
authors.initials: B
authors.lastname: Frangione
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D001609:Beta-Globulins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002556:Cerebrospinal Fluid Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D006801:Humans; D007114:Immunization; D015151:Immunoblotting; D019746:Intramolecular Oxidoreductases; D054834:Lipocalins; D008969:Molecular Sequence Data; D011817:Rabbits
chemical_list: D000906:Antibodies; D001609:Beta-Globulins; D002556:Cerebrospinal Fluid Proteins; D054834:Lipocalins; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase