title: Bacterial resistances to mercury and copper.
abstract: Heavy metals are toxic to living organisms. Some have no known beneficial biological function, while others have essential roles in physiological reactions. Mechanisms which deal with heavy metal stress must protect against the deleterious effects of heavy metals, yet avoid depleting the cell of a heavy metal which is also an essential nutrient. We describe the mechanisms of resistance in Escherichia coli to two different heavy metals, mercury and copper. Resistance of E. coli to mercury is reasonably well understood and is known to occur by transport of mercuric ions into the cytoplasmic compartment of the bacterial cell and subsequent reductive detoxification of mercuric ions. Recent mutational analysis has started to uncover the mechanistic detail of the mercuric ion transport processes, and has shown the essential nature of cysteine residues in transport of Hg(II). Resistance to copper is much less well understood, but is known to involve the increased export of copper from the bacterial cell and modification of the copper; the details of the process are still being elucidated. Expression of both metal resistance determinants is regulated by the corresponding cation. In each case the response enables the maintenance of cellular homeostasis for the metal. The conclusions drawn allow us to make testable predictions about the regulation of expression of resistance to other heavy metals.
authors.affiliation: Microbial Molecular Genetics and Cell Biology Research Group, School of Biological Sciences, University of Birmingham, United Kingdom.
authors.forename: N L
authors.initials: NL
authors.lastname: Brown
authors.forename: J
authors.initials: J
authors.lastname: Camakaris
authors.forename: B T
authors.initials: BT
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Williams
authors.forename: A P
authors.initials: AP
authors.lastname: Morby
authors.forename: J
authors.initials: J
authors.lastname: Parkhill
authors.forename: D A
authors.initials: DA
authors.lastname: Rouch
mesh_terms: D000595:Amino Acid Sequence; D003300:Copper / Q000378:metabolism / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006706:Homeostasis / Q000187:drug effects; D007473:Ion Channels; D008628:Mercury / Q000378:metabolism / Q000494:pharmacology*; D008954:Models, Biological; D008969:Molecular Sequence Data
chemical_list: D007473:Ion Channels; D003300:Copper; D008628:Mercury