Estimation of interleukin 6 production by reverse transcriptase-polymerase chain reaction in four human myeloma cell lines.
Although an autocrine growth mechanism through interleukin 6 has been advocated in human myeloma cells, reports of IL-6 production by cells from established myeloma cell lines are rare. In the present study, we examined whether or not a minute amount of interleukin 6 is produced in 4 human myeloma cell lines. IL-6 production was not detected in any of the 4 lines by enzyme immunoassay, bioassay with two interleukin 6-dependent murine hybridoma cell lines and Northern hybridization. However, we detected interleukin 6 mRNA in one (U266) of the 4 lines by the reverse transcriptase-polymerase chain reaction. Nevertheless, the proliferation of all 4 lines was not inhibited by an anti-interleukin 6 antibody. These results suggest that autocrine stimulation by interleukin 6 is not involved in the majority of human myeloma cell lines.
Suzuki
A
A
Second Department of Internal Medicine, Kyoto University, Japan.
Takahashi
T
T
Okuno
Y
Y
Fukumoto
M
M
Fukui
H
H
Koishihara
Y
Y
Ohsugi
Y
Y
Ohno
Y
Y
Imura
H
H
D001483:Base Sequence; D015152:Blotting, Northern; D002455:Cell Division; D002460:Cell Line; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism
D015850:Interleukin-6; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase