Identification of mRNA for epidermal growth factor and transforming growth factor-alpha present in low copy number in human endometrium and decidua using reverse transcriptase-polymerase chain reaction.
The presence of mRNA for epidermal growth factor (EGF) and transforming growth factor-alpha (TGF alpha) was demonstrated in small fragments of human endometrium and decidua by use of the technique of reverse transcriptase-polymerase chain reaction with nested oligonucleotide primers. The presence of mRNA encoding EGF and TGF alpha has not been shown in human endometrium previously. Other studies using conventional techniques, such as Northern blot or in-situ hybridization, showed the presence in low copy number of EGF but not TGF alpha in murine endometrium. Messenger RNA for EGF was not present in peripheral leukocytes or platelets, suggesting an endometrial source for the message. Messenger RNA for TGF alpha was found in these blood components, thus preventing confirmation of the source of TGF alpha mRNA.
Haining
R E
RE
Department of Obstetrics & Gynaecology, Rosie Maternity Hospital, Cambridge.
Schofield
J P
JP
Jones
D S
DS
Rajput-Williams
J
J
Smith
S K
SK
D001483:Base Sequence; D004247:DNA; D003656:Decidua / Q000737:chemistry*; D004717:Endometrium / Q000737:chemistry*; D004815:Epidermal Growth Factor / Q000235:genetics*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D016211:Transforming Growth Factor alpha / Q000235:genetics*
D012333:RNA, Messenger; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor; D004247:DNA; D012194:RNA-Directed DNA Polymerase