title: Suppression of messenger ribonucleic acid for glutathione peroxidase in chemically induced rat hepatocellular carcinoma and its biological significance.
abstract: Deviations of the enzyme activity, immunoreactivity and messenger ribonucleic acid (mRNA) levels of glutathione peroxidase (GSH-PO) in 3'-methyl-4-dimethylaminoazobenzene- induced hepatocellular carcinoma of the rat were investigated. Enzyme activities of GSH-PO were significantly lower in hepatocellular carcinomas than those in the normal control rat liver. Immunohistochemically, GSH-PO was strongly localized in normal hepatocytes, but was only faintly stained in hepatocellular carcinoma cells. Heterogeneous staining patterns of GSH-PO were observed among individual cancer cells. In Northern blot analysis, GSH-PO mRNA in the cancer tissue was decreased to two thirds of the level in normal hepatocytes. It was suggested that suppressed expression of GSH-PO in carcinogen-induced hepatocellular carcinomas occurred at the level of mRNA transcription.
authors.affiliation: Department of Pathology, School of Medicine, Tokai University, Kanagawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Lertprasertsuke
authors.forename: M
authors.initials: M
authors.lastname: Shinoda
authors.forename: S
authors.initials: S
authors.lastname: Takekoshi
authors.forename: S
authors.initials: S
authors.lastname: Yoshimura
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D005979:Glutathione Peroxidase / Q000235:genetics*; D007150:Immunohistochemistry / Q000379:methods; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D008749:Methyldimethylaminoazobenzene; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
chemical_list: D012333:RNA, Messenger; D008749:Methyldimethylaminoazobenzene; D005979:Glutathione Peroxidase