title: A method for histological preparation of undecalcified bone sections containing acrylic bone cement.
abstract: An improved and time reducing method is presented for the histological evaluation of bone containing polymethylmethacrylate (PMMA) bone cement. The undecalcified bone was embedded in epoxy resin and section of 50-100 microns thickness were produced using a commercially available cutting grinding system. The sections were stained with Stevenel's blue and van Gieson picrofuchsin or a modified hematoxylineosin. PMMA bone cement was not dissolved and remained enabling examination in situ of an intact cement bone interface and tissue reaction without decalcification.
authors.affiliation: Department of Orthopedic Surgery, Rigshospitalet, Denmark.
authors.forename: L N
authors.initials: LN
authors.lastname: Jensen
authors.forename: J S
authors.initials: JS
authors.lastname: Jensen
authors.forename: K
authors.initials: K
authors.lastname: Gotfredsen
mesh_terms: D000818:Animals; D001843:Bone Cements; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000473:pathology / Q000502:physiology; D004285:Dogs; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D006651:Histocytochemistry; D008768:Methylmethacrylates; D013194:Staining and Labeling
chemical_list: D001843:Bone Cements; D008768:Methylmethacrylates; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin