Cell-induced potentiation of the plasminogen activation system is abolished by a monoclonal antibody that recognizes the NH2-terminal domain of the urokinase receptor.
We have raised four monoclonal antibodies recognizing different epitopes within the human cell-surface receptor for urokinase-type plasminogen activator (u-PA). One of these antibodies completely abolishes the potentiation of plasmin generation observed upon incubation of the zymogens pro-u-PA and plasminogen with U937 cells. This antibody, which is also the only one to completely inhibit the binding of DFP-inactivated [125I]-u-PA to U937 cells, is directed against the u-PA binding NH2-terminal domain of u-PAR, a well-defined fragment formed by limited chymotrypsin digestion of purified u-PAR, demonstrating the functional independence of the u-PA binding domain as well as the critical role of u-PAR in the assembly of the cell-surface plasminogen activation system.
Rønne
E
E
Finsen Laboratory, Rigshospitalet, Copenhagen, Denmark.
Behrendt
N
N
Ellis
V
V
Ploug
M
M
Danø
K
K
Høyer-Hansen
G
G
D000911:Antibodies, Monoclonal; D002460:Cell Line; D002918:Chymotrypsin / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D005341:Fibrinolysin / Q000032:analysis; D006801:Humans; D007700:Kinetics; D010958:Plasminogen / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism*; D011233:Precipitin Tests; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D055293:Receptors, Urokinase Plasminogen Activator; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D000939:Epitopes; C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D010958:Plasminogen; D010960:Plasminogen Activators; D002918:Chymotrypsin; D005341:Fibrinolysin