[Detection of cytomegalovirus in urine by the shell-vial technique: usefulness of diluting the sample].
To assess the toxicity of urine samples on human fibroblast cell monolayer using Shell-vial technique for cytomegalovirus antigen detection. A total of 227 urine samples were processed, inoculated on Shell-vial simultaneously, being either diluted (1:1) and non diluted in transport media. After 24-48 hours incubation, the fibroblast cells monolayer was stained using fluorescein-marked monoclonal anti-cytomegalovirus antibodies. Cytomegalovirus was detected in 9.6% of samples inoculated with diluted urine, and in 5.2% of samples inoculated with non-diluted urine. Destruction of monolayer cells was seen in 13.2 and 22.4, respectively. With Shell-vial technique for detecting cytomegalovirus in urine samples, a high number of positive results was obtained using diluted urine. Also a significant minor destruction of cells monolayer was observed with the same inoculum.
Acosta
B
B
Servicio de Microbiología, Hospital La Fe, Valencia.
Ferrer
D
D
Jordán
M
M
González
D
D
Gobernado
M
M
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000382:microbiology* / Q000652:urine; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts; D006801:Humans; D016867:Immunocompromised Host; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling; D013997:Time Factors; D014556:Urine / Q000382:microbiology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral
Detección de citomegalovirus en orina por la técnica de shell-vial: valor de diluir la muestra.