Use of rapid, nonradioactive DNA probes in culture confirmation tests to detect Streptococcus agalactiae, Haemophilus influenzae, and Enterococcus spp. from pediatric patients with significant infections.
Acridinium ester-labeled, chemiluminescent DNA probe tests (Accuprobe; Gen-Probe, Inc.) for culture confirmation of Streptococcus agalactiae, Haemophilus influenzae, and Enterococcus spp. were compared with conventional identification techniques. The probe is a DNA oligomer that is a complementary to the RNA of the target. The DNA-RNA hybrids are measured in a luminometer. The 40-min assay requires one reaction tube and the addition of three reagents. When two colonies were used to add a sample of the reaction tube, 325 of 327 isolates were detected by the probe. Isolates of 404 nonprobe target organisms did not hybridize with the probe.
Daly
J A
JA
Primary Children's Medical Center, University of Utah Medical Center, Salt Lake City 84113.
Clifton
N L
NL
Seskin
K C
KC
Gooch
W M
WM
D002675:Child, Preschool; D015342:DNA Probes; D004269:DNA, Bacterial / Q000378:metabolism; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000302:isolation & purification*; D006801:Humans; D012329:RNA, Bacterial / Q000378:metabolism; D013290:Streptococcal Infections / Q000382:microbiology*; D013291:Streptococcus / Q000302:isolation & purification*; D013292:Streptococcus agalactiae / Q000302:isolation & purification*
D015342:DNA Probes; D004269:DNA, Bacterial; D012329:RNA, Bacterial