A histochemical study of changes observed in the mouse diaphragm after organophosphate poisoning.
A sublethal dose of sarin (GB, isopropyl methylphosphonofluoridate) was administered to mice. The animals were killed up to 28 d after dosing and frozen sections were made of the excised diaphragms which were stained using haematoxylin and eosin and a modified Gomori trichrome method. Muscle fibre degeneration and mononuclear infiltration were seen, notably at 24 h and 3 d. A number of histochemical procedures were carried out, including the GBHA procedure for ionized calcium. Calcium accumulation, seen at 4 h, was the earliest abnormality observed. All changes were rapidly regressing by 5 d and histological appearances were normal by 14 d. It was concluded that sarin produced myopathic changes preceded by calcium accumulation.
Bright
J E
JE
CDE Porton Down, Salisbury, Wilts, UK.
Inns
R H
RH
Tuckwell
N J
NJ
Griffiths
G D
GD
Marrs
T C
TC
D000110:Acetylcholinesterase / Q000187:drug effects; D000135:Acid Phosphatase / Q000187:drug effects; D000251:Adenosine Triphosphatases / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003094:Collagen / Q000187:drug effects; D003964:Diaphragm / Q000187:drug effects; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012524:Sarin / Q000506:poisoning*; D013194:Staining and Labeling / Q000379:methods
D003094:Collagen; D012524:Sarin; D000110:Acetylcholinesterase; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases; D002118:Calcium