Analysis of the human type I insulin-like growth factor receptor promoter region.
We isolated genomic fragments containing the 5' region of the human type I insulin-like growth factor receptor gene. A unique transcription start site was identified, defining a 1038 bp 5'-untranslated region. No TATA or CCAAT elements were identified in the proximal 480 nucleotides of 5'-flanking region. The region surrounding the transcription start site was similar to a recently described "initiator" sequence. The 5'-flanking and 5'-untranslated regions were highly GC-rich, with numerous potential Sp1 binding sites. A potential AP-2 binding site was identified in the 5'-flanking region and a potential thyroid response element was identified in the 5'-untranslated region. The 5' region of the human gene was very similar to that of the rat gene, with conservation of many of the potential regulatory elements.
Cooke
D W
DW
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Bankert
L A
LA
Roberts
C T
CT
LeRoith
D
D
Casella
S J
SJ
D000818:Animals; D001483:Base Sequence; D005260:Female; D015698:Genomic Library; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007438:Introns; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010920:Placenta / Q000502:physiology; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics*; D017451:Receptors, Somatomedin; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D011061:Poly A; D012313:RNA; D007334:Insulin-Like Growth Factor I