Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle.
During the course of an investigation on nuclear matrix protein cDNAs, we have isolated a cDNA clone hybridizing with the messenger RNA encoding mitotin. Mitotin is a 125 kDa/pI 6.5 nuclear matrix protein present in proliferating but not in resting cells. This protein was shown to have a marked increase and characteristic redistribution in G2/M phase of the cell cycle. In this report, using synchronized Raji and WISH cells, we demonstrate that mitotin messenger RNA is expressed at the same level throughout the cell cycle.
Todorov
I T
IT
Institut of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia.
Lavigne
J
J
Sakr
F
F
Kaneva
R
R
Foisy
S
S
Bibor-Hardy
V
V
D015152:Blotting, Northern; D002453:Cell Cycle; D002460:Cell Line; D003001:Cloning, Molecular; D006801:Humans; D015530:Nuclear Matrix / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics*; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
D009687:Nuclear Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA