Cytomegalovirus induced PMN adherence in relation to an ELAM-1 antigen present on infected endothelial cell monolayers.
In human umbilical vein endothelial cells infected with cytomegalovirus (CMV), an activation antigen recognized by monoclonal antibody (mAb) ENA1 appeared. mAb ENA1 reacts with an inducible endothelial surface antigen which has characteristics similar to those of ELAM-1. Incubation with anti-IL-1 partly inhibited this appearance and, parallel to this, the virus-induced polymorphonuclear cell (PMN) adhesion was decreased. In addition, the adhesion of PMN to virus-infected endothelial cells could be reduced by F(ab)2 fragments of mAb ENA1 to almost control level. The results obtained after incubation of PMN with mAb IB4 (against CD18) suggest that the adhesion of PMN to uninfected endothelial cells is CD18 glycoprotein dependent, and virus infection up-regulates this glycoprotein-dependent mechanism. These results indicate that the virus-induced PMN adhesion is regulated by the following mechanism: virus infection of endothelial cells induces IL-1 production, and the autocrine IL-1 causes the expression of ELAM-1 on the surface of endothelial cells. In turn this activation antigen ELAM-1 binds with its putative ligand present on the PMN membrane. The virus-induced PMN adhesion occurs also through a CD18 glycoprotein-dependent mechanism.
Span
A H
AH
Department of Medical Microbiology, University of Limburg, Maastricht, The Netherlands.
Mullers
W
W
Miltenburg
A M
AM
Bruggeman
C A
CA
D015703:Antigens, CD / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D018821:CD18 Antigens; D002448:Cell Adhesion / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000276:immunology*; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000276:immunology; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000032:analysis*; D009504:Neutrophils / Q000276:immunology*; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology
D015703:Antigens, CD; D000956:Antigens, Viral; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007375:Interleukin-1; D008562:Membrane Glycoproteins; D016028:Receptors, Leukocyte-Adhesion