Multimerization of AAGTGA and GAAAGT generates sequences that mediate virus inducibility by mimicking an interferon promoter element.
Multimeric AAGTGA and GAAAGT, when inserted before a minimal promoter, mediate virus-inducible transcription. We have determined that the active sequence within these multimers is TGAAAGTGAAAGT, which is structurally similar to GAGAAGTGAAAGT, a positive response element delineated in the beta-interferon gene promoter. Both sequences behave like protoenhancers and are similar as regards induction by virus or interferon regulatory factor 1 when supported by a simian virus 40 enhancer.
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Institut für Molekularbiologie I. Universität Zürich, Switzerland.
Hardin
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Weissmann
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D000818:Animals; D001483:Base Sequence; D005914:Globins / Q000235:genetics; D007369:Interferon Inducers; D007372:Interferons / Q000235:genetics*; D007739:L Cells / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009522:Newcastle disease virus / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000494:pharmacology*; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D016385:TATA Box; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
D007369:Interferon Inducers; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D005914:Globins; D007372:Interferons