title: 5-Hydroxytryptamine releasing activity of the supernatants from cultured mouse spleen cells.
abstract: Mouse spleen cells have been shown to produce a histamine releasing factor (HRF) after stimulation with mitogen or specific antigen in vitro. The supernatants from the cultures of mouse spleen cells released not only histamine but also 5-hydroxytryptamine (5-HT) from homologous mast cells in a dose-dependent manner. The time-course of this release was similar to that observed in antigen or anti-IgE reaction. Heavy water (D2O) enhanced supernatant-induced mediator release.
authors.affiliation: Department of Biogenic Amines, Polish Academy of Sciences, Lódź.
authors.forename: Z
authors.initials: Z
authors.lastname: Sułowska
authors.forename: J
authors.initials: J
authors.lastname: Wyczółkowska
mesh_terms: D000818:Animals; D000941:Antigens; D014408:Biomarkers, Tumor; D003208:Concanavalin A / Q000276:immunology; D005260:Female; D006636:Histamine Release; D007073:Immunoglobulin E / Q000378:metabolism; D066298:In Vitro Techniques; D008222:Lymphokines / Q000096:biosynthesis; D008407:Mast Cells / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D012701:Serotonin / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism*; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D000941:Antigens; D014408:Biomarkers, Tumor; D008222:Lymphokines; D000090324:Tumor Protein, Translationally-Controlled 1; D003208:Concanavalin A; D012701:Serotonin; D007073:Immunoglobulin E; D010047:Ovalbumin