Antigenic properties associated with Vinca alkaloid resistance in ovarian cancer cells: identification of a 92,000 Da protein.
In order to characterize the membrane changes related to Vinca alkaloid resistance, we raised monoclonal antibodies (mAbs) against a Vincristine resistant subline (OV1/VCR) derived from a human ovarian adenocarcinoma cell line (OV1/p). Among three monoclonal antibodies selected for a higher binding to OV1/VCR than to OV1/p cells, one designated OVR09, recognized a Mr 92,000 protein. This protein appears to be gradually overexpressed along the drug resistance establishment in vitro, and to decrease slowly in absence of drug. Further, mAb OVR09 showed a much higher binding to the vinblastine resistant epidermoid tumor cell line KbV1 than to its parental counterpart. The Mr 92,000 protein was also detected in various tumor cell lines and in an ovarian carcinoma surgical sample.
Voeltzel
T
T
Département de Biologie Clinique, Institut Gustave Roussy, Villejuif, France.
Bénard
J
J
Lavaissiere
L
L
Solère
P
P
Da Silva
J
J
Bohuon
C
C
Bidart
J M
JM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D015153:Blotting, Western; D002277:Carcinoma / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D004351:Drug Resistance; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010051:Ovarian Neoplasms / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology*; D014750:Vincristine / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes; D014750:Vincristine