[Cloning preprolactin cDNA from Pacific salmon in Escherichia coli].
Prolactin coding mRNA was shown to be a prevalent part of chum salmon (Oncorhynchus keta) pituitary poly(A)-RNA during the spawning period. Clone lambda gtPrk12 was selected from the pituitary cDNA library by means of hybridization with the prolactin probe, and a nucleotide sequence of the insertion was determined and compared to the prolactin coding sequences from rainbow trout and Pacific chinook salmon, which had been published earlier. The sequences compared exhibited a significant homology. The deduced amino acid sequence of the chum salmon prolactin differed from a sequence determined directly in a single position. The prolactin-coding sequence can be used for constructing the bacterial strain producing prolactin.
Prokopenko
I V
IV
Lomakin
I B
IB
Zakharova
E S
ES
Vinetskiĭ
Iu P
IuP
D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics*; D011388:Prolactin / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger; D012474:Salmon; D012689:Sequence Homology, Nucleic Acid
D011498:Protein Precursors; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; C017671:preprolactin; D011388:Prolactin; D004247:DNA
Klonirovanie kDNK preprolaktina tikhookeanskogo lososia v Escherichia coli.