Doxapram inhibits the in vitro oxidation of hexobarbital.
The effect of doxapram on the mouse liver microsomal hexobarbital metabolism in vitro was studied. Doxapram inhibited hexobarbital oxidase in a competitive manner, with inhibition constants between 2 and 10 mM. Doxapram induced a reverse type I spectral change with a spectral dissociation constant of about 0.1 microM. These results indicate that doxaprame is an inhibitor of microsomal drug metabolism in the mouse.
Ishikawa
M
M
Department of Pharmacology, Tohoku College of Pharmacy, Sendai, Japan.
Ozaki
M
M
Takayanagi
Y
Y
Sasaki
K
K
D000818:Animals; D003577:Cytochrome P-450 Enzyme System; D004315:Doxapram / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D006591:Hexobarbital / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling
D003577:Cytochrome P-450 Enzyme System; D004315:Doxapram; D006591:Hexobarbital