title: Albumin and a dialyzable serum factor stimulate feeding in vitro by third-stage larvae of the canine hookworm Ancylostoma caninum.
abstract: Previous studies demonstrated that third-stage, developmentally arrested larvae of the canine hookworm Ancylostoma caninum resume feeding in vitro in response to canine serum and hostlike temperature. Experiments to determine the identity of the serum stimulus are described. Serum from several nonhost species stimulated feeding, but to levels lower than canine serum. Heating the serum to 57 C had no effect on its stimulatory ability. Dialysis reduced serum stimulatory activity by 50%, and ultrafiltration through 10- and 30-kDa molecular weight cut-off membranes decreased activity in both the filtrates and retentates similarly. Recombination of the filtrates and retentates restored activity to whole serum control levels. Commercial canine and bovine albumin stimulated feeding to serum control levels at 10 and 50 mg/ml, respectively. These results suggest that albumin and an unidentified low molecular weight compound(s) are capable of inducing in vitro feeding by A. caninum L3.
authors.affiliation: Department of Pathobiology, University of Pennsylvania, Philadelphia 19104.
authors.forename: J M
authors.initials: JM
authors.lastname: Hawdon
authors.forename: G A
authors.initials: GA
authors.lastname: Schad
mesh_terms: D000722:Ancylostoma / Q000254:growth & development*; D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D002364:Caseins / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007814:Larva; D011492:Protein Hydrolysates / Q000494:pharmacology; D012709:Serum Albumin / Q000494:pharmacology*; D005719:gamma-Globulins / Q000494:pharmacology
chemical_list: D001798:Blood Proteins; D002364:Caseins; D011492:Protein Hydrolysates; D012709:Serum Albumin; D005719:gamma-Globulins; C026375:trypticase-soy broth