In vitro HIV infection of primate lymphocytes.
Peripheral blood mononuclear cells (PBMC) obtained from four different primate species were tested for their respective ability to support the "in vitro" replication of the human immunodeficiency viruses, HIV-1, and HIV-2. PBMC of Cebus apella, patas (Erythrocebus patas), green (cercopithecus aethiops sabaeus) and rhesus monkeys (Macaca mulatta) were infected "in vitro" with either HIV-1 or HIV-2. Cultures were assayed weekly for particle-associated reverse transcriptase activity. Both viruses were found to be cytolytic for all these monkey's PBMC. Low levels of HIV-1 and HIV-2 infection were observed in Cebus cells. However, productive infection was only detected in HIV-2 infected rhesus PBMC. The capacity of HIV-2 to replicate in rhesus cells may provide a useful model for evaluating antiviral drugs and vaccines.
Kraiselburd
E N
EN
Department of Microbiology and Medical Zoology, University of Puerto Rico, San Juan 00936.
Williams
D C
DC
Kessler
M J
MJ
D000818:Animals; D002428:Cebus / Q000382:microbiology; D002470:Cell Survival; D002478:Cells, Cultured; D002522:Chlorocebus aethiops / Q000382:microbiology; D004901:Erythrocebus patas / Q000382:microbiology; D015497:HIV-1 / Q000502:physiology*; D015498:HIV-2 / Q000502:physiology*; D000882:Haplorhini / Q000382:microbiology*; D007963:Leukocytes, Mononuclear / Q000201:enzymology / Q000382:microbiology*; D008253:Macaca mulatta / Q000382:microbiology; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D014779:Virus Replication
D012194:RNA-Directed DNA Polymerase