Isolation of Yersinia-specific T cell clones from the synovial membrane and synovial fluid of a patient with reactive arthritis.
Synovial fluid (SF) mononuclear cells from patients with reactive arthritis (ReA) proliferate in vitro when challenged with ReA-associated bacteria, the maximal response being for the organism causing the triggering infection. We report the results of a study of the antigenic specificity of synovial T lymphocytes from an HLA-B27 positive ReA patient whose SF mononuclear cells responded preferentially to Yersinia antigens. This is the first report of the isolation of Yersinia-specific T cell clones from synovial membrane (obtained by closed-needle synovial biopsy). We present a detailed analysis of these clones, together with others obtained from the SF.
Viner
N J
NJ
Department of Rheumatology, University of Birmingham, United Kingdom.
Bailey
L C
LC
Life
P F
PF
Bacon
P A
PA
Gaston
J S
JS
D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology; D001170:Arthritis, Infectious / Q000097:blood / Q000473:pathology*; D016918:Arthritis, Reactive / Q000382:microbiology / Q000473:pathology*; D001707:Biopsy, Needle; D002469:Cell Separation; D000939:Epitopes; D015796:HLA-B27 Antigen / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D008297:Male; D010641:Phenotype; D000091402:Prohibitins; D013582:Synovial Fluid / Q000166:cytology* / Q000382:microbiology; D013583:Synovial Membrane / Q000382:microbiology / Q000473:pathology*; D013601:T-Lymphocytes / Q000382:microbiology / Q000473:pathology*; D015007:Yersinia / Q000276:immunology* / Q000302:isolation & purification; D015009:Yersinia Infections / Q000382:microbiology / Q000473:pathology*
D000907:Antibodies, Bacterial; D000939:Epitopes; D015796:HLA-B27 Antigen; D000949:Histocompatibility Antigens Class II; C000717331:PHB2 protein, human; D000091402:Prohibitins