Characterization of nitric oxide synthases in non-adrenergic non-cholinergic nerve containing tissue from the rat anococcygeus muscle.
Tissue homogenates prepared from rat anococcygeus muscle converted L-arginine to L-citrulline indicating the presence of nitric oxide (NO) synthase. NO synthase activity was also found in crude and partially-purified soluble and particulate fractions prepared from the homogenates. Both soluble and particulate NO synthase were dependent on NADPH, 5,6,7,8-tetrahydrobiopterin and calcium, and inhibited by NG-nitro-L-arginine. Tissue homogenates or crude cytosolic and membrane fractions from rat vas deferens, which does not contain NO releasing non-adrenergic non-cholinergic neurones, had no NO synthase activity.
Mitchell
J A
JA
Abbott Laboratories, Abbott Park, Illinois 60064.
Sheng
H
H
Förstermann
U
U
Murad
F
F
D000594:Amino Acid Oxidoreductases / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D001708:Biopterins / Q000031:analogs & derivatives; D002118:Calcium; D002956:Citrulline / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000201:enzymology; D066298:In Vitro Techniques; D008297:Male; D009132:Muscles / Q000201:enzymology* / Q000294:innervation; D019001:Nitric Oxide Synthase; D019335:Nitroarginine; D051381:Rats; D011919:Rats, Inbred Strains; D014649:Vas Deferens / Q000201:enzymology
D019335:Nitroarginine; D001708:Biopterins; D002956:Citrulline; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; C003402:sapropterin; D002118:Calcium