Biochemical and developmental characterization of the murine cluster of differentiation 1 antigen.
The cluster of differentiation-1 (CD1) antigens are major histocompatibility complex (MHC) class I-like glycoproteins belonging to the immunoglobulin supergene family. Initially described in humans, more recently putative CD1 encoding genes have been identified in several other species, including the mouse where it has been clearly demonstrated that CD1 mRNA is expressed. However, in the mouse both its unusually wide tissue distribution and the prevalence of incompletely spliced RNA have raised the possibility that the mRNA did not encode a functional protein. We have utilized a rabbit polyclonal antiserum raised against an Escherichia coli-expressed recombinant murine CD1 fusion protein to characterize the murine CD1 protein. Here we demonstrate that the antiserum binds specifically to a set of glycoproteins (49,000-55,000 MW) which contain a common core protein with both a size (36,000 MW) and tissue distribution in accordance with those predicted. During thymic ontogeny, this protein is highly expressed by Day 14 of embryonic development and persists into adulthood, while its pattern of expression in other organs changes significantly during development. Thus, the mouse provides an amenable model system for the study of CD1 function.
Mosser
D D
DD
National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec.
Duchaine
J
J
Martin
L H
LH
D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000737:chemistry*; D018949:Antigens, CD1; D015153:Blotting, Western; D005260:Female; D007106:Immune Sera; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D013950:Thymus Gland / Q000196:embryology / Q000254:growth & development / Q000276:immunology
D015703:Antigens, CD; D018949:Antigens, CD1; D007106:Immune Sera