title: An improved method for isolating RNA from porcine adipose tissue.
abstract: In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 M guanidium thiocyanate, 25 mM sodium citrate, 0.5% Sarcosyl, 0.1 M beta-mercaptoethanol, pH 7.0, and then performing two CHCl3 extractions to remove lipid before following the procedure described by P. Chomczynski and N. Sacchi (1987, Anal. Biochem. 162, 156-159). This modification improved the yield of RNA approximately threefold (yield was 88 +/- 7 micrograms total RNA/g of tissue) without affecting RNA quality.
authors.affiliation: Institut National de la Recherche Agronomique, Station de Recherches Porcines, Saint Gilles, L'Hermitage, France.
authors.forename: I
authors.initials: I
authors.lastname: Louveau
authors.forename: S
authors.initials: S
authors.lastname: Chaudhuri
authors.forename: T D
authors.initials: TD
authors.lastname: Etherton
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D012313:RNA / Q000302:isolation & purification*; D013552:Swine
chemical_list: D012313:RNA