Induction of immune response to bovine herpesvirus-1 with anti-idiotypic antibodies.
Previously, we prepared rabbit anti-idiotypic (anti-Id) antibodies against murine monoclonal antibodies (MAbs) specific for the major bovine herpesvirus-1 (BHV-1) envelope glycoproteins. Glycoprotein III (gIII) contains neutralization epitopes and may be the virus attachment protein. Anti-Id antibodies to a neutralizing MAb that reacts with gIII were purified by sequential immunoaffinity chromatography. Immune responses to the purified anti-Id reagent and BHV-1 were compared in mice. Both groups of mice produced BHV-1-specific neutralizing antibodies. However, lymphocyte proliferative responses and interferon and interleukin-2 production were specific for the respective immunizing antigens. These results suggest that the anti-Id reagent may bear an internal image of a B-cell-stimulating epitope of glycoprotein gIII; however, this epitope does not stimulate a virus-specific cellular immune response in mice.
Orten
D J
DJ
Department of Laboratory Medicine, Kansas State University, Manhattan.
Reddy
P G
PG
Reddy
D N
DN
Xue
W
W
Abdelmagid
O Y
OY
Blecha
F
F
Minocha
H C
HC
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006564:Herpesviridae / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology*; D007372:Interferons / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D007376:Interleukin-2; D014759:Viral Envelope Proteins; D007372:Interferons