Cyclic variations in the permeability of the cell wall of Saccharomyces cerevisiae.
To study cell-cycle-related variations in wall permeability of Saccharomyces cerevisiae, two approaches were used. First, an asynchronous culture was fractionated by centrifugal elutriation into subpopulations containing cells of increasing size. The subpopulations represented different stages of the cell cycle as judged by light microscopy. Cell wall porosity increased when these subpopulations became enriched with budded cells. Secondly, synchronous cultures were obtained by releasing MATa cells from alpha-factor induced G1-arrest. These cultures grew synchronously for at least two generations. The cell wall porosity increased sharply in these cultures, shortly before buds became visible and was maximal during the initial stages of bud growth. It decreased in cells which had completed nuclear migration and before abscission of the bud had occurred. The porosity reached its lowest value during abscission and in unbudded cells. We examined the incorporation of mannoproteins into the wall during the cell cycle. SDS-extractable mannoproteins were incorporated continuously. However, the incorporation of glucanase-extractable mannoproteins, which are known to affect cell wall porosity, showed cyclic oscillations and reached its maximum after nuclear migration. This coincided with a rapid decrease in cell wall porosity, indicating that glucanase-extractable mannoproteins might contribute to this decrease.
De Nobel
J G
JG
University of Amsterdam, Molecular Cell Biology, Biotechnology Center, The Netherlands.
Klis
F M
FM
Ram
A
A
Van Unen
H
H
Priem
J
J
Munnik
T
T
Van Den Ende
H
H
D001557:Benzenesulfonates; D002453:Cell Cycle; D002463:Cell Membrane Permeability; D002473:Cell Wall / Q000378:metabolism; D005591:Chemical Fractionation; D008562:Membrane Glycoproteins / Q000378:metabolism; D016062:Porosity; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D013194:Staining and Labeling
D001557:Benzenesulfonates; D008562:Membrane Glycoproteins; C025363:mannoproteins; C007061:C.I. Fluorescent Brightening Agent 28