Stimulation of growth of human breast cancer cells (T47D) by platelet derived growth factor.
The human breast cancer cell lines T47D and MCF-7, respond to the mitogenic action of exogenously added PDGF with a 2-3-fold increase in cell number. The maximal response was obtained at a concentration of 1.25 half maximal units/ml (125 ng/ml). PDGF-AA was even more effective than PDGF-AB while PDGF-BB was without effect. The growth-enhancing activity of PDGF was completely abolished by Fab fragments of anti PDGF. Within 7 min of addition of PDGF to cultures of T47D cells, specific phosphorylation of a 65-kDa protein was observed. T47D cells contain specific receptors for PDGF with approximately 4-7 x 10(4) sites (kDa of 3-4 x 10(-10) M) per cell.
Ginsburg
E
E
Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, MD 20892.
Vonderhaar
B K
BK
D017302:Annexins; D000906:Antibodies / Q000494:pharmacology; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002135:Calcium-Binding Proteins / Q000037:antagonists & inhibitors; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D010766:Phosphorylation; D019000:Phosphotyrosine; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D015398:Signal Transduction; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014443:Tyrosine / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors
D017302:Annexins; D000906:Antibodies; D002135:Calcium-Binding Proteins; D007140:Immunoglobulin Fab Fragments; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D019000:Phosphotyrosine; D014443:Tyrosine; D017479:Receptors, Platelet-Derived Growth Factor