Neuropeptide Y mRNA regulation in rat sympathetic ganglia: effect of reserpine.
To study the mechanism underlying trans-synaptic neuropeptide regulation, mRNA levels of neuropeptide Y were examined in the rat stellate and superior cervical ganglia using a specific neuropeptide Y cRNA probe. Basal levels of neuropeptide Y mRNA were detectable in total RNA extracts from single ganglia. Reserpine induced a large rise in ganglion neuropeptide Y mRNA. Decentralization prevented the increase of neuropeptide Y mRNA content in the ganglia. This suggests that the reserpine induced increase in neuropeptide Y mRNA was dependent on transsynaptic stimulation. Consequently, neuropeptide mRNA levels in sympathetic ganglia may be under the control of preganglionic impulse flow.
Hänze
J
J
Department of Physiology and Pathophysiology, Univesity of Marburg, F.R.G.
Kummer
W
W
Haass
M
M
Lang
R E
RE
D001339:Autonomic Fibers, Preganglionic / Q000502:physiology*; D004790:Enzyme Induction; D005728:Ganglia, Sympathetic / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D009478:Neuropeptide Y / Q000096:biosynthesis*; D012313:RNA; D018075:RNA, Complementary; D012333:RNA, Messenger / Q000096:biosynthesis; D012110:Reserpine / Q000494:pharmacology*; D013562:Sympathectomy; D014158:Transcription, Genetic / Q000187:drug effects; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis
D009478:Neuropeptide Y; D018075:RNA, Complementary; D012333:RNA, Messenger; D012313:RNA; D012110:Reserpine; D014446:Tyrosine 3-Monooxygenase