Applications of confocal microscopy to the study of myelin development and neuron structure.
Confocal laser scanning microscopy has been used to study the localization of myelin basic proteins expressed in nonglial cells, and to probe the three-dimensional structure of central auditory neurons in the lateral superior olive. The paper focuses on the techniques used to obtain the results. The key roles of confocal microscopy and computer image processing of the images obtained are emphasized as they relate to the discovery of essential structural information about these specimens.
Staugaitis
S M
SM
Department of Cell Biology, NYU Medical Center, New York 10016.
Rowan
T
T
Sanes
D H
DH
Colman
D R
DR
Smith
P R
PR
D000818:Animals; D005849:Gerbillinae; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008856:Microscopy, Fluorescence; D004676:Myelin Basic Protein / Q000378:metabolism*; D009474:Neurons / Q000166:cytology*; D009836:Oligodendroglia / Q000378:metabolism*; D011149:Pons / Q000166:cytology; D000159:Vestibulocochlear Nerve / Q000166:cytology
D004676:Myelin Basic Protein