Effect of PUVA radiation on anaphylactic histamine release from rat dermal tissues.
We have devised a new in vitro model of type I cutaneous anaphylaxis. Male albino rats were sensitized with DNP-Ascaris. Abdominal skin was shaved, and thin, split-thickness slices of skin were cut with a dermatome. The dermis was excised and cut into 100 mg pieces. The dermal tissue was incubated with antigen in Tyrode's solution for 30 min at 37 degrees C. Antigen-induced histamine release from dermal tissue was measured fluorimetrically. Using this system, we measured histamine release from PUVA-irradiated and non-irradiated dermal tissues. A single PUVA irradiation inhibited type I cutaneous anaphylaxis, but did not affect spontaneous histamine release or total dermal histamine. Our model is considered to be useful for investigation of the mechanism of suppression of type I cutaneous anaphylaxis by PUVA.
Tanii
T
T
Department of Dermatology, Osaka City University Medical School, Japan.
Kono
T
T
Mizuno
N
N
Furukawa
M
M
Nagahama
K
K
Someda
Y
Y
Hamada
T
T
Shibata
T
T
Okada
T
T
D000707:Anaphylaxis / Q000209:etiology / Q000378:metabolism*; D000818:Animals; D006636:Histamine Release / Q000187:drug effects / Q000528:radiation effects*; D066298:In Vitro Techniques; D008297:Male; D011701:PUVA Therapy; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects / Q000528:radiation effects; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000378:metabolism*