Identification of a cDNA clone specific for the oligodendrocyte-derived repulsive extracellular matrix molecule J1-160/180.
A cDNA clone specific for the oligodendrocyte-derived extracellular matrix glycoproteins J1-160/180 was obtained from a lambda ZAPII expression library using polyclonal antibodies generated against mouse J1-160. The library was constructed from poly(A)(+)-RNA isolated from O1 antigen-positive rat oligodendrocytes. The cDNA clone expressed a fusion protein that was recognized by the J1-160/180-specific monoclonal antibodies 596, 619, and 620, and, weakly, 597. The fusion protein was not recognized by polyclonal antibodies to mouse J1/tenascin. The cDNA clone with an insert of approximately 5.6 kb in size contained the nucleotide sequence coding for the amino acid sequence of the N-terminus of a tryptic peptide derived from mouse J1-160. The developmental and tissue distribution of the mRNA recognized by the cDNA clone is in agreement with the described expression of the J1-160/180 proteins.
Fuss
B
B
Department of Neurobiology, Swiss Federal Institute of Technology, Hönggerberg.
Pott
U
U
Fischer
P
P
Schwab
M E
ME
Schachner
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D015723:Gene Library; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009836:Oligodendroglia / Q000502:physiology*; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000032:analysis; D015183:Restriction Mapping; D019063:Tenascin
D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D019063:Tenascin; C074638:tenascin R; D011061:Poly A; D012313:RNA; D004247:DNA