Translation in vitro of codon UGA as tryptophan in Mycoplasma capricolum.
The in-frame UGA codons in the synthetic messenger RNA were translated in the cell-free system of Mycoplasma capricolum. The result, together with the occurrence of codon UGA at tryptophan sites in the genes and the presence of tRNA(UCATrp) pairing with UGA, clearly indicated that UGA is a tryptophan codon in this bacterium.
Oba
T
T
Department of Biology, School of Science, Nagoya University, Japan.
Andachi
Y
Y
Muto
A
A
Osawa
S
S
D001483:Base Sequence; D003062:Codon / Q000235:genetics*; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012364:RNA, Transfer, Trp / Q000235:genetics; D014364:Tryptophan / Q000235:genetics*
D003062:Codon; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012364:RNA, Transfer, Trp; D014364:Tryptophan