title: [Preliminary results of the DGTI Workshop for evaluating the reactivity of monoclonal anti-D].
abstract: Within a working group of the German transfusion society, a workshop was conducted to evaluate the reactivity of monoclonal anti-D supernatants. During this evaluation 10,000 samples were tested, including 325 Du-samples, with 3-5 IgM type antibodies and 3-4 IgG type antibodies, which were selected to also find 'D-variants'. In general, the reactivity with D+ red blood cells was good, and specific in respect to D negative samples. Some samples have been found to have a different pattern of reactivity than the monoclonal antibodies used. Dependent on the method and on the antibody, in some studies up to 100% of Du samples could be detected by anti-D of the IgM type. Some data indicate that the reaction with Du samples is not dependent on titer. In addition, in separate studies the data show a correlation of reactivity in respect to the Rh-phenotype (CcDuee greater than ccDuEe).
authors.affiliation: Biotest AG, Forschungszentrum, Offenbach.
authors.forename: H H
authors.initials: HH
authors.lastname: Sonnenborn
authors.forename: C
authors.initials: C
authors.lastname: Darda
authors.forename: M
authors.initials: M
authors.lastname: Ernst
authors.forename: W A
authors.initials: WA
authors.lastname: Flegel
authors.forename: W
authors.initials: W
authors.lastname: Helmbold
authors.forename: D
authors.initials: D
authors.lastname: Kasulke
authors.forename: P
authors.initials: P
authors.lastname: Kühnl
authors.forename: C
authors.initials: C
authors.lastname: Mueller-Eckhardt
authors.forename: H
authors.initials: H
authors.lastname: Northoff
authors.forename: A
authors.initials: A
authors.lastname: Poschmann
mesh_terms: D000911:Antibodies, Monoclonal; D005858:Germany; D006801:Humans; D007518:Isoantibodies; D018029:Rho(D) Immune Globulin; D012955:Societies, Medical
chemical_list: D000911:Antibodies, Monoclonal; D007518:Isoantibodies; C061961:RHO(D) antibody; D018029:Rho(D) Immune Globulin
vernacular_title: Vorläufige Ergebnisse des DGTI Workshops zur Evaluierung der Reaktivität von monoklonalem Anti-D.