Two populations of glial cells from fish optic nerve/tract with distinct electrophysiological properties.
The electrophysiological properties of the two major glial cell types in cultures from the regenerating goldfish optic nerve/tract were studied with patch-clamp techniques. Spindle-shaped cells express myelin proteins. These oligodendrocyte-like cells possess outwardly rectifying currents, do not show glutamate activated currents and are rarely electrically coupled to neighboring cells. Cells of epitheloid morphology probably represent astrocytes. They are GFAP-positive and do not exhibit myelin proteins. These cells have glutamate activated currents, display a linear current to voltage relationship and are extensively electrically coupled thus displaying properties similar to mammalian astrocytes.
Hoppe
D
D
Department of Neurobiology, University of Heidelberg, Federal Republic of Germany.
Bastmeyer
M
M
von Blankenfeld
G
G
Kettenmann
H
H
Stuermer
C A
CA
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D004594:Electrophysiology / Q000379:methods; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006054:Goldfish; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D009185:Myelin Proteins / Q000032:analysis; D009416:Nerve Regeneration; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology; D009900:Optic Nerve / Q000166:cytology / Q000502:physiology*; D014795:Visual Pathways / Q000166:cytology / Q000502:physiology*
D005904:Glial Fibrillary Acidic Protein; D005971:Glutamates; D007473:Ion Channels; D009185:Myelin Proteins; D018698:Glutamic Acid; D015761:4-Aminopyridine