Biomarkers in styrene-exposed boatbuilders.
14 fiberglass-reinforced plastics (FRP) boatbuilders were compared with 9 unexposed controls with respect to several chemical specific and nonspecific biomarkers measured in peripheral blood. Biomarkers included styrene-hemoglobin adducts (styrene-Hb), sister-chromatid exchanges (SCEs), micronuclei (MN), single-strand breaks (SSBs) and N-acetoxy-2-acetylaminofluorene-induced DNA binding (NA-AAF binding) as a measure of susceptibility to DNA damage. Workers' exposures averaged 11 ppm (8-h TWA; geometric mean) and ranged from 0.6 to 44 p.p.m. Mandelic acid levels were measured in end-of-shift urine samples and reflected an average styrene exposure equivalent to 15 p.p.m. There was a large though not significant difference in levels of styrene-Hb adducts among exposed workers and controls, largely the consequence of a single heavily-exposed individual with an extremely high level of adducts. Significant differences between biomarker levels in exposed workers and controls were observed with MN, SSBs and NA-AAF binding. No significant differences were seen in mean levels of SCEs nor in the incidence of cells with a high frequency of SCEs. The data suggest that exposure to levels of styrene in occupational settings near or below the current OSHA standard (50 p.p.m.) can induce damage at the cellular/molecular level. Appropriately-selected panels of biomarkers can be useful in identifying potentially harmful exposures.
Brenner
D D
DD
Division of Environmental Sciences, Columbia University School of Public Health, New York, NY 10032.
Jeffrey
A M
AM
Latriano
L
L
Wazneh
L
L
Warburton
D
D
Toor
M
M
Pero
R W
RW
Andrews
L R
LR
Walles
S
S
Perera
F P
FP
D000099:Acetoxyacetylaminofluorene / Q000378:metabolism; D000328:Adult; D015415:Biomarkers / Q000097:blood; D002869:Chromosome Aberrations; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D004784:Environmental Monitoring; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000473:pathology; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D012767:Ships; D012854:Sister Chromatid Exchange; D020058:Styrene; D013343:Styrenes / Q000009:adverse effects* / Q000097:blood / Q000378:metabolism
D015415:Biomarkers; D006454:Hemoglobins; D013343:Styrenes; D020058:Styrene; D000099:Acetoxyacetylaminofluorene; D004247:DNA