[Immunohistochemistry and morphometry of bone marrow in acquired immunodeficiency syndrome with special emphasis on megakaryopoiesis and macrophages].
An immunomorphometric study was performed on formalin-fixed and paraffin-embedded bone marrow biopsies in 20 patients with AIDS (18 males, 2 females-stage IV A-D). In comparison with a control group megakaryocytes (CD61-Y2/51) revealed not only a significant hyperplasia, but remarkably irregular shapes of cells and nuclei, together with a disturbance of the nuclear-cytoplasmic ratio. No predominance of micromegakaryocytes as in myelodysplastic syndromes was observable. Contrasting idiopathic (immune)-thrombocytopenia, HIV-infected patients with a pronounced depression of the platelet count did not show a significant elevation of the number of promegakaryoblasts. This feature is in keeping with findings of a severe impairment of progenitor cell proliferation and differentiation in AIDS. There was a pronounced increase in the macrophage population (PG-M1). This alteration may be related to inflammatory lesions accompanying this disorder as well as to an enforced and premature destruction of hematopoietic cell elements in the myeloid stroma.
Thiele
J
J
Institute für Pathologie, Universität zu Köln.
Titius
B
B
Dienemann
D
D
Falini
B
B
Wagner
S
S
Stein
H
H
Fischer
R
R
D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology*; D000328:Adult; D001853:Bone Marrow / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D005260:Female; D015658:HIV Infections / Q000473:pathology*; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000473:pathology*; D008297:Male; D008533:Megakaryocytes / Q000473:pathology*; D010976:Platelet Count; D013921:Thrombocytopenia / Q000097:blood / Q000473:pathology
Immunhistochemie und Morphometrie am Knochenmark bei Immunodefektsyndrom unter besonderer Berücksichtigung von Megakaryopoiese und Makrophagen.