GABA inhibits the rise in cytosolic free calcium concentration in depolarized immature cerebellar Purkinje cells.
gamma-Aminobutyric acid (GABA) reduced the peak rise or slowed the rate of rise in cytosolic free calcium concentration ([Ca]in) induced by quisqualate, kainate, or high KCl in immature cerebellar Purkinje cell bodies. The sustained rise or repeated transient of [Ca]in induced by tetraethylammonium, veratridine, or Bay-K-8644 was lowered to the basal level by adding GABA, although the inhibition by GABA of Bay-K-8644-induced rise in [Ca]in was only slight and transient in some cells. These findings indicate that GABA inhibits the rise in [Ca]in by hyperpolarizing the membrane potentials at Purkinje cell bodies.
Sorimachi
M
M
Department of Physiology, Kagoshima University School of Medicine, Japan.
Yamagami
K
K
Morita
Y
Y
Kuramoto
K
K
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000375:Aging; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002531:Cerebellum / Q000254:growth & development*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D005711:Gallopamil / Q000494:pharmacology; D007700:Kinetics; D011689:Purkinje Cells / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000187:drug effects / Q000502:physiology*; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D011963:Receptors, GABA-A; D013757:Tetraethylammonium Compounds; D005711:Gallopamil; D013779:Tetrodotoxin; D005680:gamma-Aminobutyric Acid; D019789:Tetraethylammonium; D014701:Veratridine; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D002118:Calcium