Effect of human immunodeficiency virus type 1 on CD34+ cells.
The effect of HIV-1 on the in vitro growth of enriched hematopoietic stem cells (CD34+ cells) obtained from normal peripheral blood samples was studied. In comparison to untreated controls, the number of viable CD34+ cells progressively and significantly decreased in liquid cultures containing interleukin-3 (IL-3, 100 U/ml) after inoculation with HIV-1. In inoculated samples there was a significant reduction of all the hematopoietic progenitors (CFU-GM, BFU-E, CFU-Meg) starting from the second day of culture, CFU-GM being the most affected. In spite of these findings, no evidence of viral replication was observed: the total amount of p24 in HIV-1-inoculated CD34+ cell cultures showed a plateau, slightly declining towards the end of the experimental observation period. Moreover, erythroid and granulomacrophage colonies harvested from inoculated CD34+ cell cultures were unable to infect susceptible cells.
Zauli
G
G
Institute of Histology and General Embryology, University of Bologna, Italy.
Re
M C
MC
Giovannini
M
M
Bagnara
G P
GP
Furlini
G
G
Ranieri
S
S
La Placa
M
M
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D015497:HIV-1 / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000382:microbiology / Q000502:physiology; D006801:Humans; D007962:Leukocytes / Q000166:cytology* / Q000382:microbiology / Q000502:physiology; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34