Molecular cloning, structure and expression analysis of a full-length mouse brain glutamate dehydrogenase cDNA.
We isolated and analysed a full-length mouse brain glutamate dehydrogenase (GLUD) cDNA as a preliminary step to use the mouse model for the investigation of GLUD function in neurotransmission and neurodegeneration. GLUD coding sequences were found highly conserved among mouse, human and rat. Northern blots revealed two transcripts with different ratios in different mouse organs implying some mechanism of tissue-specific expression. In contrast to human, mouse GLUD gene family appears not to contain an intronless member.
Tzimagiorgis
G
G
Department of Biology, University of Crete, Greece.
Moschonas
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NK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001923:Brain Chemistry; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis; D051381:Rats; D014158:Transcription, Genetic
D012313:RNA; D004247:DNA; D005969:Glutamate Dehydrogenase