Evidence that Fluoro-Gold can be transported avidly through fibers of passage.
Small iontophoretic injections of the retrograde tracer Fluoro-Gold were restricted to the dorsal columns in the cervical enlargement of 6 rats. Large numbers of neurons were labeled in the lumbosacral dorsal horn in each rat. In the most effective case, more than 1800 neurons were labeled in alternate sections through nine examined segments. Many neurons were also labeled in lumbosacral dorsal root ganglia of all cases. This study, in contrast to previous reports, indicates that Fluoro-Gold can be transported avidly by axons passing through, but not terminating in, injection sites.
Dado
R J
RJ
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 55455.
Burstein
R
R
Cliffer
K D
KD
Giesler
G J
GJ
D000818:Animals; D001370:Axonal Transport; D001692:Biological Transport / Q000502:physiology; D005456:Fluorescent Dyes; D007267:Injections; D007478:Iontophoresis; D008297:Male; D009336:Necrosis; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines