X-ray microanalysis of cAMP-induced ion transport in cystic fibrosis fibroblasts.
cAMP-induced ion transport in normal and cystic fibrosis (CF) fibroblasts was investigated by X-ray microanalysis. Stimulation with cAMP causes an increase in cellular Na content and a decrease in cellular Cl and K content. No significant difference in response between CF and normal cells was noted. In this respect, fibroblasts differ from epithelial cells, where cAMP-induced Cl- efflux blocked in CF patients. Isoproterenol produced similar changes in Na and K content as cAMP, but did not effect Cl content.
von Euler
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A
Department of Human Anatomy, University of Uppsala, Sweden.
Roomans
G M
GM
D002478:Cells, Cultured / Q000187:drug effects; D002713:Chlorine / Q000032:analysis; D000242:Cyclic AMP / Q000494:pharmacology*; D003550:Cystic Fibrosis / Q000378:metabolism*; D004577:Electron Probe Microanalysis; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology*; D011188:Potassium / Q000032:analysis; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000032:analysis
D007473:Ion Channels; D002713:Chlorine; D012964:Sodium; D000242:Cyclic AMP; D007545:Isoproterenol; D011188:Potassium