title: Characterization of epithelial cell cultures derived from human tracheal glands.
abstract: Cultures of normal human tracheal gland epithelial cells that exhibit functional differentiation have been propagated in serum-free medium supplemented with insulin (5 micrograms/ml), epidermal growth factor (10 ng/ml), hydrocortisone (0.5 micrograms/ml), and bovine pituitary extract (25 micrograms/ml). The cells retain many characteristics of epithelial cells including microvilli on cell surfaces, desmosomes between cells, and tonofilaments in the cytoplasm. In addition, they exhibit keratin-positive titers and react positively with Peanut agglutinin, which is specific for the disaccharide beta-D-galactose-(1----3)N-acetyl D-galactosamine, a major component of mucin glycoprotein. The cells also exhibit normal Cl- channel activity which was enhanced by the cAMP agonist Forskolin. The major component of the cellular secretion was hyaluronic acid; approximately 10% of the void volume material was resistant to hyaluronidase and may contain material similar to mucin glycoprotein. Some of the cell cultures have been maintained in serum-free conditions for 6 to 7 passages. This model will be important to study regulation of ion-channel activities and mucous glycoprotein secretion and to compare such regulations with the tracheal mucosal epithelial cells already established.
authors.affiliation: Institute of Chemical Toxicology, Wayne State University, Detroit, Michigan 48201.
authors.forename: D P
authors.initials: DP
authors.lastname: Chopra
authors.forename: R L
authors.initials: RL
authors.lastname: Shoemaker
authors.forename: G W
authors.initials: GW
authors.lastname: Taylor
authors.forename: P A
authors.initials: PA
authors.lastname: Mathieu
mesh_terms: D002240:Carbohydrate Sequence; D002455:Cell Division; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D005088:Exocrine Glands / Q000166:cytology* / Q000502:physiology; D005944:Glucosamine / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007473:Ion Channels / Q000502:physiology; D007700:Kinetics; D008564:Membrane Potentials; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009077:Mucins / Q000096:biosynthesis; D009093:Mucus; D010902:Pituitary Gland / Q000502:physiology; D012694:Serine / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D014020:Tissue Extracts / Q000494:pharmacology; D014132:Trachea / Q000166:cytology*
chemical_list: D007328:Insulin; D007473:Ion Channels; D009077:Mucins; D013431:Sulfates; D014020:Tissue Extracts; D012694:Serine; D004815:Epidermal Growth Factor; D006820:Hyaluronic Acid; D005944:Glucosamine; D006854:Hydrocortisone