Latex-agglutination analysis of human recombinant interleukin-2 with monoclonal antibodies.
Hybridomas producing monoclonal antibodies (Mabs) to human interleukin-2 have been obtained. The antibodies have been characterised in terms of binding constant, subclass, and cross-reaction with proteins from Escherichia coli and human blood serum. The epitope for the monoclonal antibody LNKB-2, namely the 66-72 fragment of the interleukin-2 molecule, has been located. From the antibodies obtained, two capable of reacting simultaneously with the monomeric form of interleukin-2 have been selected and a rapid simple Mab-based latex-agglutination assay which can detect as little as 1.5 ng ml-1 interleukin-2 has been developed.
Lunev
V E
VE
Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow.
Lukin YuV
Kazennykh
N V
NV
Belyaev
S V
SV
Zubov
V P
VP
Nesmeyanov
V A
VA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D000918:Antibody Specificity; D001710:Biotin; D004396:Coloring Agents; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology*; D007841:Latex Fixation Tests; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D000939:Epitopes; D007376:Interleukin-2; D011994:Recombinant Proteins; D001710:Biotin