Human galanin: primary structure and identification of two molecular forms.
From acid/ethanol extracts of surgical specimens of human large intestine we isolated two peptides, in approximately equal amounts, that reacted with an antiserum against porcine galanin. By amino acid analysis, sequence analysis and mass spectrometry, the larger of the two peptides was found to consist of 30 amino acid residues, the sequence of which was identical to that of porcine galanin except for the following substitutions: Val16, Asn17, Asn26, Thr29 and Ser30. Unlike porcine galanin, the carboxy-terminus was not amidated. The smaller peptide corresponded to the first 19 amino acid residues counted from the N-terminus of the 30 residue peptide (again without amidation). The structural analysis was repeated on another batch of tissue with identical results. By HPLC analysis of extracts of specimens from a further 4 patients, the same peptides were identified. Thus, human galanin includes two peptides of 19 and 30 amino acids that share the sequence of the N-terminal 15 residues with other mammalian galanins, but exhibit characteristic differences in the remaining part of the molecules.
Bersani
M
M
Panum Institute, University of Copenhagen, Denmark.
Johnsen
A H
AH
Højrup
P
P
Dunning
B E
BE
Andreasen
J J
JJ
Holst
J J
JJ
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000737:chemistry; D019004:Galanin; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000737:chemistry; D009479:Neuropeptides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D009479:Neuropeptides; D010455:Peptides; D019004:Galanin