Binding studies of gold labelled lectins on carbohydrate compounds of the flask cells in claw-frog kidney.
The carbohydrate compounds of the mucus of flask cells in the kidney of claw-frogs (Xenopus laevis) were studied by gold marked lectins (WGA, RCA, L, LCA, HPA, PNA). We used a post-embedding technique. Seminthin or ultrathin sections of Lowieryl K H M-embedded kidney tissue were incubated. For light microscopy, a gold-silver technique was used. The mucus of the flask cells reacted strongly with WGA, RCA L and HPA, whereas LCA and PNA showed no binding. The Golgi apparatus and small cytoplasmatic vesicles reacted also positively with WGA, RCA L and HPA. The autoradiographically detected secretion routes of the glycosaminoglycan-rich secretion of flask cells are also demonstrable by lectins.
Jonas
L
L
Electron Microscopic Centre, University of Rostock, F.R.G.
Ostwald
C
C
Walzel
H
H
D000818:Animals; D050260:Carbohydrate Metabolism; D003102:Colloids; D006025:Glycosaminoglycans; D006046:Gold / Q000378:metabolism*; D006056:Golgi Apparatus / Q000648:ultrastructure; D006735:Horseradish Peroxidase; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism; D008854:Microscopy, Electron; D013194:Staining and Labeling; D014909:Wheat Germ Agglutinins; D014982:Xenopus laevis
D003102:Colloids; D006025:Glycosaminoglycans; D037102:Lectins; D014909:Wheat Germ Agglutinins; D006046:Gold; D006735:Horseradish Peroxidase