[Cytological research on the argentophilic nucleolus-organizer regions of the metaphase chromosomes in parthenogenetic mouse embryos].
Silver staining technique visualizing argentophilic nucleolus organizer regions (Ag-NORs) was used for studying parthenogenetic mouse embryos produced by artificial activation of oocytes in Ca(2+)-Mg(2+)-free medium. Ag-NOR-containing chromosomes were detected in metaphases of parthenogenetic embryos during six successive cleavage divisions starting with the two-cell stage. The frequency of metaphases with varying AG-NOR number in diploid parthenogenones was similar to that in the control (fertilized) embryos. Average number of metaphase Ag-NOR chromosomes (calculated per diploid chromosome set) in haploid parthenogenones exceeded that in the control; in some cases all NORs were stained by silver. This is evidence that latent ribosomal cistrons in some chromosomes can be activated.
Dyban
A P
AP
Severova
E L
EL
Noniashvili
E M
EM
D000818:Animals; D004171:Diploidy; D047108:Embryonic Development; D005260:Female; D006238:Haploidy; D008677:Metaphase; D051379:Mice; D008810:Mice, Inbred C57BL / Q000196:embryology*; D008808:Mice, Inbred CBA / Q000196:embryology*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D010312:Parthenogenesis; D011247:Pregnancy; D012834:Silver; D013194:Staining and Labeling / Q000379:methods
D012834:Silver
Tsitologicheskoe issledovanie argentofil'nykh iadryshkoobrazuiushchikh raĭonov metafaznykh khromosom u partenogeneticheskikh zarodysheĭ mysheĭ.