T-cell regulation of CD5+ B-cell activity in normal mice.
The differentiation of plaque-forming cell (PFC) precursors against bromelain-treated syngeneic erythrocytes (Br MRBC) into PFC induced in vitro by LPS is down-regulated by nylon non-adherent (nylon-passed--NP) T cells and by nylon adherent (NA) T cells. NA T cells are more potent inhibitors than NP T cells. This regulatory activity of NA and NP T cells results from an interaction between CD4+ radioresistant and CD8+ radiosensitive T cells. Furthermore CD4+ T cells from the NA fraction but not from the NP fraction are activated cells: their inhibitory activity is abrogated after preincubation with cycloheximide. These results are discussed within the overall framework of T-cell regulation of autoimmune anti-Br MRBC B-cell subsets.
Huchet
R
R
Department of Immunogenetics, Paul Brousse Hospital, Villejuif, France.
Grandjon
D
D
Halle-Pannenko
O
O
D000818:Animals; D000917:Antibody Formation; D015703:Antigens, CD / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000528:radiation effects; D001402:B-Lymphocytes / Q000276:immunology*; D001963:Bromelains / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000528:radiation effects; D018956:CD5 Antigens; D016827:CD8 Antigens; D002448:Cell Adhesion; D003513:Cycloheximide / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009757:Nylons; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018956:CD5 Antigens; D016827:CD8 Antigens; D009757:Nylons; D001963:Bromelains; D003513:Cycloheximide