[Determination of apoprotein A-1 by immunoenzyme analysis].
Indirect solid-phase immunoenzymatic assay was improved and adapted for quantitative estimation of apo A-I in blood serum. Immunochemical identification of apo A-I was carried out and specific antibodies were obtained. Various procedures of blood serum pretreatment affected dissimilarly the detection of antigenic determinants in apo A-I. Presence of tetramethyl urea in blood serum increased by 30% the latent antigenic determinants accessibility.
Poliakov
L M
LM
Poteriaeva
O N
ON
Panin
L E
LE
D000906:Antibodies / Q000032:analysis; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000097:blood* / Q000276:immunology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques
D000906:Antibodies; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D000939:Epitopes
Opredelenie apoproteina A-1 metodom immunofermentnogo analiza.