title: Effects of neuropeptides, ruthenium red and neuraminidase on chemoreflexes mediated by afferents in the dog epicardium.
abstract: 1. Experiments were performed on anaesthetized, open-chest dogs to determine reflex effects on blood pressure and heart rate produced by stimulation of neural afferents of the left ventricular epicardium by local application of capsaicin, bradykinin, nicotine and the neuropeptides substance P (SP), neurokinin A (NKA), neurokinin B (NKB) and calcitonin gene-related peptide (CGRP). 2. Studies also included assessing whether reflexogenic actions of capsaicin, bradykinin and nicotine are influenced by epicardial treatment with either neuropeptides, Ruthenium Red or neuraminidase. 3. Epicardial application of either capsaicin (0.1-10 micrograms) or bradykinin (0.1-1 micrograms), consistently resulted in dose-related increases in blood pressure and heart rate, whereas reflex bradycardia and hypotensive effects were initiated by the application of nicotine (30-50 micrograms). 4. SP, NKA, NKB and CGRP caused marked hypotensive effects and tachycardia when injected intravenously (1 microgram), but failed to produce any cardiovascular response when applied to the epicardium of the left ventricle (0.1-1 microgram). Treatment of the heart surface with these neuropeptides (0.05-0.5 micrograms min-1) was also without any effect on the magnitude of reflex responses evoked by epicardial application of either capsaicin, bradykinin or nicotine. 5. Superfusion of the ventricular epicardium with Ruthenium Red (10-30 microM), a cationic dye known to have sialic acid as a molecular target, antagonized the reflexogenic effects of capsaicin but not those of bradykinin or nicotine. The reflex effects of capsaicin, but not those of bradykinin, were also sensitive to inhibition by epicardial treatment with neuraminidase, an enzyme which cleaves sialic acid residues from glycosides and sialoglycoproteins. 6. We conclude that neuropeptides which may be released from the peripheral endings of some cardiac sensory neurons neither directly activate nor sensitize spinal sympathetic and vagal afferents in the dog heart to the reflexogenic action of bradykinin, nicotine or capsaicin. 7. We further suggest that activation of the cardiac sympathetic chemoreflex by capsaicin involves its interaction with calcium-binding sialic acid moieties present on the surface of axons and/or terminals of chemosensitive sympathetic afferents distributed in the dog ventricular epicardium.
authors.affiliation: Department of Pharmacology, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Staszewska-Woolley
authors.forename: G
authors.initials: G
authors.lastname: Woolley
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001920:Bradykinin / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005260:Female; D006321:Heart / Q000294:innervation; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D009439:Neuraminidase / Q000494:pharmacology*; D009475:Neurons, Afferent / Q000187:drug effects*; D009479:Neuropeptides / Q000494:pharmacology*; D009538:Nicotine / Q000494:pharmacology; D010496:Pericardium / Q000187:drug effects*; D012018:Reflex / Q000187:drug effects; D012430:Ruthenium Red / Q000494:pharmacology*
chemical_list: D009479:Neuropeptides; D012430:Ruthenium Red; D009538:Nicotine; D009439:Neuraminidase; D002211:Capsaicin; D001920:Bradykinin