title: Nucleolar organizer regions in cardiac lesions induced by doxorubicin.
abstract: The use of the argyrophilic (Ag) staining technique for nucleolar organizer regions (NORs) revealed nuclear changes in myocytes of the left atrium of 10 rats treated twice a week for 6 weeks with doxorubicin (1 mg/kg body weight) iv and sacrificed after 6 weeks without treatment. The changes were easily detected qualitatively and further assessed by quantification. Cardiac myocytes of doxorubicin-treated rats had larger nuclei and/or a larger quantity of AgNORs that were either dispersed in a number of small dots or clustered in rounded, rod-shaped, or tortuous large structures. AgNOR alterations may reflect a defect of nucleolar association leading to an impairment of protein synthesis that could be involved in doxorubicin cardiotoxicity.
authors.affiliation: Laboratoires Pfizer, Centre de Recherche, Amboise, France.
authors.forename: B
authors.initials: B
authors.lastname: Leblanc
authors.forename: P R
authors.initials: PR
authors.lastname: Mompon
authors.forename: O
authors.initials: O
authors.lastname: Espérandieu
authors.forename: B
authors.initials: B
authors.lastname: Geffray
authors.forename: C
authors.initials: C
authors.lastname: Guillermo
mesh_terms: D000818:Animals; D009202:Cardiomyopathies / Q000139:chemically induced* / Q000473:pathology; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D004317:Doxorubicin / Q000633:toxicity*; D006321:Heart / Q000187:drug effects*; D008297:Male; D009206:Myocardium / Q000473:pathology* / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000187:drug effects / Q000648:ultrastructure*; D051381:Rats; D012834:Silver; D013194:Staining and Labeling / Q000379:methods
chemical_list: D012834:Silver; D004317:Doxorubicin