Light and electron microscopic studies of lectin binding on the glycocalyx of rat pancreatic cells. I. Normal tissue and isolated cells.
Lectin binding of the glycocalyx of pancreatic tissue sections as well as of isolated pancreatic acini and acinar cells was studied of healthy wistar rats by light and electron microscopy. For light microscopy, we used FITC (WGA, RCAI, LCA) or peroxidase marked (WGA, RCAI, PNA, PHA, LCA, UEAI, LPA) as well as unmarked lectins (Con A, VAA I). Gold marked lectins were used for electron microscopy (WGA, RCAI, LCA, HPA, PNA, VAAI-B). Intact acinar cells in pancreatic tissue sections and isolated acini showed a strong binding of WGA, RACI, and HPA on the apical cell surface, whereas VAAI, UEAI, LCA, and Con A reacted strongly with the basolateral glycocalyx, but not with the apical surface. The 2 main domains of the glycocalyx of pancreatic cells showed their specific lectin binding so long as the junctional complexes between the cells are intact. The polarity of the cell surface of pancreatic acinar cells is discussed in regard to the possible function of the 2 domains.
Jonas
L
L
Department of Pathology, University of Rostock, Germany.
Ostwald
C
C
Griethe
W
W
Letko
G
G
D000818:Animals; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006735:Horseradish Peroxidase; D037102:Lectins; D008297:Male; D008854:Microscopy, Electron; D010179:Pancreas / Q000166:cytology* / Q000648:ultrastructure; D011134:Polysaccharides / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins
D006023:Glycoproteins; D037102:Lectins; D011134:Polysaccharides; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D006735:Horseradish Peroxidase