Subclones of C6 rat glioma cells differing in intermediate filament protein expression.
The C6 rat glioma cell line is shown to consist of a mixed population of cells which either contain vimentin (80% of the cells) or completely lack any cytoplasmic intermediate filament (IF) proteins. Subclones could be established with both phenotypes, indicating that these IF protein expression patterns represent stable phenotypic markers. Absence of IF proteins in C6 subclones could consistently be correlated with an altered cell morphology and a pronounced increase in the number of actin stress fibers. In vitro translation and hybridization assays suggest the absence of vimentin to result from a block at the transcriptional level. The data indicate that subcloning of the C6 cell line on the basis of IF protein expression seems to be a reasonable approach for obtaining homogeneous C6 cell populations which may represent suitable experimental models for studies on vimentin expression and glioma cell differentiation.
Röser
K
K
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Germany.
Bohn
W
W
Giese
G
G
Mannweiler
K
K
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002455:Cell Division; D002999:Clone Cells; D003599:Cytoskeleton / Q000648:ultrastructure; D015870:Gene Expression; D005910:Glioma; D007381:Intermediate Filament Proteins / Q000235:genetics*; D007700:Kinetics; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D014746:Vimentin / Q000235:genetics* / Q000302:isolation & purification
D007381:Intermediate Filament Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014746:Vimentin; D011061:Poly A; D012313:RNA