title: Effect of vasopressin on myocardial capillary recruitment and coronary blood flow in the anesthetized rabbit.
abstract: The effects of infusion of arginine vasopressin (20 mU.kg-1.min-1) on coronary blood flow and the proportion of the coronary microvasculature perfused was studied in rabbit myocardium. Fluorescein isothiocyanate--dextran was injected into anesthetized open-chest rabbits to identify the perfused vessels and an alkaline phosphatase stain was employed to locate the total microvasculature. Coronary blood flow (radioactive microspheres) was studied in separate groups of rabbits. Vasopressin infusion caused bradycardia (243 +/- 19 to 165 +/- 22 beats/min, mean +/- SD) and an increase in mean blood pressure (92 +/- 18 to 104 +/- 12 mmHg) (1 mmHg = 133.32 Pa). Coronary blood flow decreased significantly with vasopressin from 209 +/- 68 to 97 +/- 36 mL.min-1.100 g-1. The proportion of the arteriolar bed per millimeter squared perfused decreased significantly after vasopressin from 54 +/- 13 to 44 +/- 21%, while the percentage of capillaries per millimeter squared increased significantly from 57 +/- 6 to 67 +/- 11%. There were no subepicardial versus subendocardial differences in any measured parameter. Thus, both coronary blood flow and the proportion of the arteriolar bed perfused decreased with vasopressin. However, compensation occurred in that the proportion of capillaries perfused increased. This indicated an independent level of control of the coronary arteriolar and capillary beds. These microvascular changes may help to maintain oxygen supply-demand balance with vasopressin in the heart.
authors.affiliation: Department of Physiology and Biophysics, Robert Wood Johnson Medical School, Piscataway, NJ 08854-5635.
authors.forename: W
authors.initials: W
authors.lastname: Fuhr
authors.forename: K
authors.initials: K
authors.lastname: Russo
authors.forename: H R
authors.initials: HR
authors.lastname: Weiss
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000693:Anaerobiosis; D000758:Anesthesia; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D001160:Arterioles / Q000187:drug effects; D001784:Blood Gas Analysis; D002196:Capillaries / Q000187:drug effects*; D003326:Coronary Circulation / Q000187:drug effects*; D003911:Dextrans; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins; D006439:Hemodynamics / Q000187:drug effects; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology; D011817:Rabbits
chemical_list: D003911:Dextrans; D005452:Fluoresceins; C015219:fluorescein isothiocyanate dextran; D001127:Arginine Vasopressin; D000469:Alkaline Phosphatase; D016650:Fluorescein-5-isothiocyanate