Inhibition of reverse transcription by unmodified and modified antisense oligodeoxynucleotides.
We used oligodeoxynucleotides to prevent reverse transcription of beta-globin mRNA by reverse transcriptase of avian myeloblastosis virus. Unmodified oligomers hybridized to the template arrested synthesis of cDNA in a dose dependent manner. The longer the oligomer the more efficient the inhibition, 50% inhibition being achieved at 0.3 and 30 microM of a 17- or a 12-mer, respectively. The use of complementary oligonucleotides with a 3' end blocked either by a dideoxy residue or by a dodecanol group also induced inhibition of cDNA synthesis.
Boiziau
C
C
Laboratoire de Biophysique Moléculaire, INSERM CJF 90-13, Université de Bordeaux II, France.
Blonski
C
C
Thuong
N T
NT
Shire
D
D
Toulme
J J
JJ
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D004247:DNA / Q000096:biosynthesis; D005914:Globins / Q000235:genetics*; D007700:Kinetics; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D011817:Rabbits; D018894:Reverse Transcriptase Inhibitors; D014158:Transcription, Genetic / Q000187:drug effects
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D018894:Reverse Transcriptase Inhibitors; D005914:Globins; D004247:DNA