A monoclonal antibody recognizing the C-terminal region of chicken egg white riboflavin carrier protein terminates early pregnancy in mice.
In order to identify the functionally relevant epitopes on chicken riboflavin carrier protein, we have raised monoclonal antibodies to the vitamin carrier. One of these, 6B2C12, was found to interact specifically with a synthetic oligopeptide corresponding to the C-terminal 17 amino acid residues of the chicken egg white riboflavin carrier protein, which is missing in part in the egg yolk riboflavin carrier protein. This epitope is conserved through evolution in mammals including humans. Administration of the ascites fluid of 6B2C12 to pregnant mice intraperitoneally, resulted in the termination of pregnancy indicating that this epitope is involved in or closely associated with the transplacental transport of the vitamin from the maternal circulation to the growing fetus.
Karande
A A
AA
Center for Reproductive Biology and Molecular Endocrinology, Department of Biochemistry, Indian Institute of Science, Bangalore.
Velu
N K
NK
Adiga
P R
PR
D000028:Abortion, Induced / Q000379:methods; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000737:chemistry / Q000276:immunology*; D004529:Egg White / Q000032:analysis; D000939:Epitopes; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D007116:Immunization, Passive; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D012256:Riboflavin; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D000939:Epitopes; D006023:Glycoproteins; D026901:Membrane Transport Proteins; D010455:Peptides; C019079:riboflavin-binding protein; D012256:Riboflavin