Deficiency of complement decay-accelerating factor (DAF, CD55) in non-Hodgkin's lymphoma.
We have assessed levels of surface-expressed complement regulatory proteins, decay-accelerating factor (DAF) and membrane cofactor protein (MCP) on cells from patients with hematological malignancies. Neither malignant cells nor unaffected nucleated blood cells from the patients lacked MCP. On the other hand, complete deficiency of DAF was found in 2/10 of non-Hodgkin's lymphoma (NHL), while none of the 38 patients with acute nonlymphocytic leukemia (ANLL) (14 cases), chronic myelogenous leukemia (CML) (6 cases), acute lymphocytic leukemia (ALL) (12 cases) and chronic lymphocytic leukemia (CLL) (6 cases) lacked DAF. The two patients with DAF-negative NHL had no history of paroxysmal nocturnal hemoglobinuria (PNH), and their peripheral blood cells were DAF-positive. One DAF-negative NHL exhibited T cell markers and the other those of B cell. In both cases, treatment of the DAF-negative lymphoma cells with antibody against MCP (M177) followed by Mg(2+)-EGTA-serum resulted in efficient deposition of homologous C3. These results infer that some NHL specifically lack DAF and, through treatment with M177, are targeted by homologous C3.
Fukuda
H
H
Department of Immunology, Center for Adult Diseases, Osaka, Japan.
Seya
T
T
Hara
T
T
Matsumoto
M
M
Kinoshita
T
T
Masaoka
T
T
D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD; D000954:Antigens, Surface / Q000276:immunology; D001798:Blood Proteins / Q000172:deficiency; D018958:CD55 Antigens; D003176:Complement C3 / Q000276:immunology; D003169:Complement Inactivator Proteins / Q000172:deficiency*; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007951:Leukemia, Myeloid / Q000276:immunology; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology*; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000032:analysis; D008565:Membrane Proteins / Q000172:deficiency*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D001798:Blood Proteins; C497857:CD46 protein, human; D018958:CD55 Antigens; D003176:Complement C3; D003169:Complement Inactivator Proteins; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D008565:Membrane Proteins