title: Biased VH gene expression in murine CD5 B cells results from age-dependent cellular selection.
abstract: Flow cytometry-purified, peritoneal and splenic CD5+ and CD5- B cells from neonatal and adult C57BL/6 mice were studied for expression of VH and Vx gene families in RNA colony blot assays, and for frequencies of clones secreting antibodies to bromelain-treated mouse red blood cells (BrMRBC), single-stranded DNA, trimethyl ammonium and bovine gamma-globulin, by limiting dilution. The results show few overall differences between the two B cell subsets, which both manifest ontogenic D-proximal VH preferences that are lost with age. Biased VH11 expression in CD5 B cells is high in adult peritoneum and spleen but absent in newborns. It only partly correlates with the selection of anti-BrMRBC reactivity, which is considerably higher in peritoneum than in spleen. No particular Vx bias was observed in any of the populations studied with the possible exception of Vx22 in peritoneal CD5+ B cells. We conclude that the antibody repertoire expressed by peritoneal CD5+ B cells of adult mice is not the result of a genetic program, but rather the consequence of local, age-dependent cellular selection mechanisms.
authors.affiliation: Unité d'Immunobiologie, Institut Pasteur, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Andrade
authors.forename: F
authors.initials: F
authors.lastname: Huetz
authors.forename: P
authors.initials: P
authors.lastname: Poncet
authors.forename: V
authors.initials: V
authors.lastname: Thomas-Vaslin
authors.forename: M
authors.initials: M
authors.lastname: Goodhardt
authors.forename: A
authors.initials: A
authors.lastname: Coutinho
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D015703:Antigens, CD / Q000096:biosynthesis*; D001202:Ascitic Fluid / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D018956:CD5 Antigens; D005434:Flow Cytometry; D015870:Gene Expression; D015151:Immunoblotting; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis*; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis*; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology*; D012313:RNA / Q000032:analysis; D013154:Spleen / Q000276:immunology
chemical_list: D015703:Antigens, CD; D018956:CD5 Antigens; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D012313:RNA