Intracellular chloride activity in cultured mesangial cells.
Glomerular mesangial cells require Cl ions for the development of a variety of metabolic and functional properties. In the present study the electrochemical distribution for Cl- was examined in cultured rat mesangial cells with Cl(-)-sensitive intracellular microelectrodes. It was determined that the intracellular Cl activity exceeded the levels predicted for a passively distributed ion. This was further substantiated by exposing mesangial cells to 10(-5) M bumetanide which drove intracellular Cl to a value close to electrochemical equilibrium. We conclude that Cl accumulates in mesangial cells, against its electrochemical gradient, through a transport pathway that is highly sensitive to bumetanide.
Mallis
L
L
Nephrology Division, Brooklyn Veterans Administration Hospital, N.Y.
Guber
H
H
Adler
S G
SG
Palant
C E
CE
D000818:Animals; D002034:Bumetanide / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism*; D004594:Electrophysiology / Q000379:methods; D005920:Glomerular Mesangium / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials; D051381:Rats; D011919:Rats, Inbred Strains
D002712:Chlorides; D002034:Bumetanide