Recognition of disparate HA and NS1 peptides by an H-2Kd-restricted, influenza specific CTL clone.
CTLs (CD8+) are known to recognize exogenous peptide in the context of class I MHC molecules. We observed that an influenza subtype H1 and H2 cross-reactive CTL clone B7, which was stimulated by a fusion protein containing a portion of HA2 subunit of A/PR/8 virus HA, recognized a synthetic peptide (residues 515-526) of the HA2 subunit of A/PR/8 virus strain. This CTL clone also recognized a structurally disparate NS1 peptide 50-68 of the same A/PR/8 virus. We examined the recognition of the NS1 peptide 50-68 and the HA peptide 515-526 by the subcloned CTL clone, B7-B7. Cold target inhibition experiments showed that the recognition of the HA peptide by the CTL clone B7-B7 could be competed by NS1 peptide-treated target cells and vice versa. The recognition of both NS1 peptide and HA peptide by the CTL clone B7-B7 was restricted by the same allele, H2Kd. In addition, this NS1 peptide requires approximately a 600-fold higher concn for optimal CTL recognition than did the HA peptide. We conclude that the TCR on clone B7-B7 recognizes the HA peptide or the NS1 peptide as comparable complexes with the same class I MHC molecules, although there is no obvious homology in the primary sequences of HA 515-526 and NS1 50-68 peptides. CTLs elicited with certain antigens appear to recognize distinctively different antigens complexed to the same presenting MHC molecule.
Kuwano
K
K
Department of Medicine, University of Massachusetts Medical School, Worcester 01655.
Reyes
V E
VE
Humphreys
R E
RE
Ennis
F A
FA
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002213:Capsid / Q000737:chemistry / Q000276:immunology*; D002999:Clone Cells; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D006183:H-2 Antigens / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014758:Viral Core Proteins / Q000737:chemistry / Q000276:immunology*; D017361:Viral Nonstructural Proteins
D000939:Epitopes; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D006389:Hemagglutinins, Viral; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins