Murine model of leptomeningeal dissemination using human medulloblastoma cells.
An experimental model of meningeal dissemination was developed by intracisternal inoculation of human medulloblastoma (ONS-76) cells into nude mice. All mice died within 65 days after inoculation of 1 x 10(7) tumor cells. The median survival time was 56 days. Clinical signs and histological findings were similar to those in medulloblastoma patients with meningeal dissemination. Immunohistochemical studies showed that ONS-76 cells in the subarachnoid space expressed major histocompatibility complex (MHC) class I antigens until 20 days after inoculation. After 30 days, expression of MHC class I antigens decreased and cells began to proliferate rapidly. Expression of MHC class I antigens on tumor cells may result in effective recognition by the host immune system.
Yamada
M
M
Department of Neurosurgery, Osaka University Medical School.
Shimizu
K
K
Tamura
K
K
Moriuchi
S
S
Mabuchi
E
E
Park
K C
KC
Miyao
Y
Y
Hayakawa
T
T
D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis; D006680:HLA Antigens / Q000096:biosynthesis; D006801:Humans; D008527:Medulloblastoma / Q000276:immunology / Q000473:pathology*; D008577:Meningeal Neoplasms / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008954:Models, Biological; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D013346:Subarachnoid Space; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000473:pathology / Q000637:transplantation
D000951:Antigens, Neoplasm; D006680:HLA Antigens