Effects of the membrane potential upon the Ca(2+)- and cumene hydroperoxide-induced permeabilization of the inner mitochondrial membrane.
A protonophore-induced delta psi decrease in a 180-140 mV range causes an increase in the lag-period of Ca(2+)-induced mitochondrial permeabilization but has little effect on the cumene hydroperoxide-induced permeability transition of mitochondria. Suppression of the non-specific permeability induction seems to be mediated by an increase in [ADP] in the mitochondrial matrix. A further decrease in delta psi leads to additional suppression of the non-specific permeability as a result of a partial ruthenium red-sensitive efflux of the previously accumulated Ca2+. On the other hand, complete dissipation of delta psi causes immediate induction of the non-specific permeability. It is concluded that only complete dissipation of delta psi caused by H+ leakages may act as a trigger for non-specific permeability induction.
Novgorodov
S A
SA
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, USSR.
Gudz
T I
TI
Kushnareva
Y E
YE
Eriksson
O
O
Leikin
Y N
YN
D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D001555:Benzene Derivatives / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000502:physiology*; D008933:Mitochondrial Swelling / Q000187:drug effects; D010539:Permeability; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology; D013367:Submitochondrial Particles / Q000187:drug effects / Q000502:physiology*
D001555:Benzene Derivatives; D012430:Ruthenium Red; D001278:Atractyloside; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D002122:Calcium Chloride; C007164:cumene hydroperoxide; C003853:carboxyatractyloside