Endothelin-1 of canine basilar artery in vasospasm.
Cerebral vasospasm was induced in adult mongrel dogs by a two-hemorrhage method. The basilar arteries were quickly frozen after careful removal of surrounding blood clot and their level of immunoreactive endothelin-1, a strong vasoconstrictor produced by the endothelial and vascular smooth-muscle cells, was measured by sandwich-enzyme immunoassay. The levels of immunoreactive endothelin-1 (mean +/- standard deviation) were 112.9 +/- 7.0 pg/mg protein prior to vasospasm, 180.4 +/- 24.7 pg/mg protein on Day 2 after vasospasm, and 115.0 +/- 24.0 pg/mg protein on Day 7, showing a significant increase (p less than 0.01) in immunoreactive endothelin-1 only on Day 2. In addition, vasospasm was moderately reversed by the topical application of monoclonal antibody against endothelin-1 on Day 2 but rather resistant to topical monoclonal antibody on Day 7. It is suggested that endothelin-1 could act as a trigger in the early stages of cerebral vasospasm, but that the maintenance of cerebral vasospasm at later stages might be independent of endothelin-1.
Yamaura
I
I
Department of Neurosurgery, Hyogo College of Medicine, Japan.
Tani
E
E
Maeda
Y
Y
Minami
N
N
Shindo
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D001488:Basilar Artery / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D016232:Endothelins / Q000378:metabolism* / Q000502:physiology; D007124:Immunoenzyme Techniques; D002546:Ischemic Attack, Transient / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D016232:Endothelins