[Synthesis of RNA using T7 RNA polymerase and immobilized DNA in a stream type reactor].
The DNA ligase-induced assembly of synthetic oligodeoxyribonucleotides on polymer supports was used to obtain immobilized DNA, containing the T7 RNA polymerase promoter and coding for a 14-membered oligoribonucleotide. The obtained template can carry out RNA synthesis in a flowing-type reactor. Sepharose 4B and Toyopearl HW-55 were used as supports.
Elov
A A
AA
Volkov
E M
EM
Shabarova
Z A
ZA
D001483:Base Sequence; D002853:Chromatography, Liquid; D004247:DNA / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012313:RNA / Q000138:chemical synthesis*; D013604:T-Phages / Q000201:enzymology*; D014158:Transcription, Genetic
D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases
Sintez RNK s pomoshch'iu RNK-polimerazy T7 i immobilizovannoĭ DNK v reaktore protochnogo tipa.