Spontaneously productive C-type retrovirus infection of fish cell lines.
The spontaneous production and release of morphologically typical, 85 to 90 nm diameter C-type retrovirus particles from four cell lines derived from three species of warmwater fish have been identified. Virus pellets from cell culture supernatants showed high levels of Mn(2+)-dependent reverse transcriptase activity at 24 degrees C. Peak enzyme activity was associated with a 1 x 16 g/ml sucrose gradient fraction. All four isolates induced a cytolytic infection of a bluegill fry cell line within 6 to 10 days.
Frerichs
G N
GN
Institute of Aquaculture, University of Stirling, U.K.
Morgan
D
D
Hart
D
D
Skerrow
C
C
Roberts
R J
RJ
Onions
D E
DE
D000818:Animals; D002460:Cell Line; D005399:Fishes / Q000382:microbiology*; D010471:Perches / Q000382:microbiology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism; D014771:Virion / Q000378:metabolism
D012194:RNA-Directed DNA Polymerase