Effect of heat shock on gene expression of Aedes albopictus cells infected with Mayaro virus.
Three major Mayaro virus proteins of 62, 50 and 34 kDa were detected in Aedes albopictus cells after 48 h postinfection at 28 degrees C. When the infected cells were shifted from 28 to 37 degrees C for 90 min (heat shock conditions), the synthesis of two major heat shock proteins (HSP) 82 and 70 kDa was induced concomitantly with strong inhibition of virus and normal protein synthesis. Total cellular RNA was isolated from mock and infected cells incubated at 28 degrees C or under heat shock. Northern blot analysis with HSP genomic probes from Drosophila sp showed that (1) the probe for HSP 82 hybridized with an RNA of 2.6 kb present only in heat-shocked cells, (2) the HSP 70 probe hybridized with RNA species of 2.5 kb, present only in RNA from heat-shocked cells. These results showed that Mayaro virus was not able to alter the reprogrammation of gene expression induced by heat shock in A. albopictus cells.
da Costa Carvalho
M G
MG
Lab. do Controle da Expressão Gênica, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Fournier
M V
MV
D000330:Aedes; D000524:Alphavirus / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D012313:RNA / Q000032:analysis; D014776:Virus Cultivation
D006360:Heat-Shock Proteins; D012313:RNA