T cell can recognize the allospecificities formed by the substitution of amino acids associated with HLA-Bw4/Bw6 public epitopes.
Our previous studies clearly showed that HLA-B35 and HLA-Bw53 differed only by the amino acids associated with HLA-Bw4/Bw6 epitopes, in that the former possessed Bw6 and the latter Bw4 epitope. It remains to be known whether T cell can discriminate HLA-B35 from HLA-Bw53, although the difference between these HLA antigens is discriminated by monospecific human alloantisera. To investigate allorecognition of these HLA antigens by T cells, anti-HLA-Bw53 cytotoxic T lymphocytes (CTLs) were generated. Anti-HLA-Bw53 cytotoxic T lymphocytes (CTLs) were generated. Anti-HLA-Bw53 bulk CTLs from an individual with HLA-B35 clearly discriminated HLA-Bw53 from HLA-B35. On the other hand, anti-HLA-Bw53 bulk CTLs from an individual without HLA-B35 revealed weak cross-reactivity with HLA-B35 and HLA-B51. The additional studies using HLA-Bw53 or HLA-B35-specific CTL clones showed that some but not all of the CTL clones definitively distinguish the difference between HLA-Bw53 and HLA-B35. Thus, the allospecificities formed by HLA-Bw4/Bw6 epitopes were discriminated by allogeneic T cells. The present study demonstrated that HLA-Bw4/Bw6 public epitopes play an important role in allorecognition of T cells.
Hiraiwa
M
M
Department of Immunology, University of Tokyo, Japan.
Yamamoto
J
J
Matsumoto
K
K
Karaki
S
S
Nagao
T
T
Kano
K
K
Takiguchi
M
M
D000595:Amino Acid Sequence; D002461:Cell Line, Transformed; D000939:Epitopes / Q000276:immunology*; D006680:HLA Antigens; D015235:HLA-B Antigens / Q000276:immunology*; D015797:HLA-B35 Antigen / Q000276:immunology; D006801:Humans; D007519:Isoantigens / Q000276:immunology*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000939:Epitopes; D006680:HLA Antigens; D015235:HLA-B Antigens; D015797:HLA-B35 Antigen; C078438:HLA-B53 antigen; C042388:HLA-Bw4 antigen; C038652:HLA-Bw6 antigen; D007519:Isoantigens