A simple and rapid experimental protocol for studies of nucleic acids metabolism and their base composition.
A suitable, simple and rapid protocol for metabolic studies of nucleic acids and determining their base composition, using reversed-phase high-performance liquid chromatography is described. Modified classic methods of isolation of the nucleic acids fraction from a biological material, in our particular case Artemia sp., were used. Then analysis of their constituents and the incorporated radioactivity, after hydrolytic processes, was performed by high-performance liquid chromatography under isocratic conditions, with 9 min total retention time. This method may be applied in several aspects of nucleic acids research, such as molecular cloning or metabolic and phylogenetic studies.
Liras
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Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
D000818:Animals; D001156:Artemia / Q000737:chemistry; D001482:Base Composition; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D009696:Nucleic Acids / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism
D009696:Nucleic Acids; D012313:RNA; D004247:DNA