title: Binding of S protein by Neisseria gonorrhoeae and potential role in invasion.
abstract: An agglutination assay was used to examine the binding of purified human S protein (vitronectin, serum spreading factor) to 201 clinical isolates of Neisseria gonorrhoeae. Strains belonging to the protein IA serovars were significantly (P less than 0.001) more reactive in agglutination tests with human S protein and were more serum resistant than strains belonging to the protein IB serovars. The strains from patients with disseminated infections belonged predominantly to the IA serovar (19 of 23) and, with the exception of IA-4 and certain IB serovars, avidly agglutinated with S protein. The serovar IA-4 and IB strains isolated from joint or cerebrospinal fluid failed to agglutinate with S protein and appeared to be less serum resistant than most other IA isolates. Cysteine hydrochloride or 2-mercaptoethanol inhibited agglutination of S protein and a more than twofold increase in resistance to killing by fresh human serum following preincubation with S protein; the serum-sensitive parent strain did not agglutinate S protein, and serum resistance was not increased following preincubation with this protein. Binding of S protein by gonococci may represent a novel pathogenic mechanism that can contribute to serum resistance.
authors.affiliation: Division of Sexually Transmitted Diseases Laboratory Research, Centers for Disease Control, Atlanta, Georgia 30333.
authors.forename: R J
authors.initials: RJ
authors.lastname: Arko
authors.forename: C Y
authors.initials: CY
authors.lastname: Chen
authors.forename: W O
authors.initials: WO
authors.lastname: Schalla
authors.forename: S K
authors.initials: SK
authors.lastname: Sarafian
authors.forename: C L
authors.initials: CL
authors.lastname: Taylor
authors.forename: J S
authors.initials: JS
authors.lastname: Knapp
authors.forename: S A
authors.initials: SA
authors.lastname: Morse
mesh_terms: D000371:Agglutination / Q000187:drug effects; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000378:metabolism*; D003545:Cysteine / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism*; D006069:Gonorrhea / Q000382:microbiology; D006801:Humans; D008623:Mercaptoethanol / Q000494:pharmacology; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000378:metabolism*; D014169:Transformation, Bacterial; D019096:Vitronectin
chemical_list: D001798:Blood Proteins; D006023:Glycoproteins; D019096:Vitronectin; D008623:Mercaptoethanol; D003545:Cysteine