[Detection of erythrocyte antibodies using solid phase assays].
The use of solid-phase assays for the detection of irregular antibodies facilitates automatization and rationalization in blood group serology. We compared the solid screen assay, (SP-assay, Biotest AG, Frankfurt, FRG) with conventional assays for antibody screening. The SP assay was as sensitive as the conventional indirect antibody test (IAT). Whereas only 4% of cold-reactive antibodies detected in enzyme tests showed positive results in the SP-assay, this test produced unspecific positive reactions in 7% of all tested sera. We found two additional antibodies with the SP-assay, not detectable in conventional techniques (anti-D, immune anti-A).
Beckmann-Emami
E
E
Abt. für Transfusionmedizin und Transplantationsimmunologie Universität Hamburg.
Emami
M
M
Kühnl
P
P
Lliger
C
C
D001788:Blood Grouping and Crossmatching / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007518:Isoantibodies / Q000032:analysis*
D007518:Isoantibodies
Nachweis Erythrozytärer Antikörper mit Hilfe von Festphasenplatten.