Influence of lindane on the fluidity of the rat ventral prostate membranes.
The influence of lindane upon the dynamic properties of plasma membranes from rat ventral prostate has been investigated using a fluorescence polarization technique. Preincubation with lindane decreased the fluorescence polarization in a dose dependent manner. This effect, which is associated with an increased membrane fluidity, occurred in a very short period of time. Lindane also provoked a number of changes in lipid biosynthesis from acetate in the membrane. Less [1-14C]acetate was incorporated into cholesterol and more into phospholipids when this liposoluble toxicant was added to the preincubation medium. However, not all phospholipid classes were equally increased, because while the rate of acetate incorporation was greater into choline glycerophospholipids than into ethanolamine glycerophospholipids, both were higher than the rates of acetate incorporation into serine glycerophospholipids and sphingomyelin.
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Departmento de Bioquímica y Biología Molecular, Universidad de Alcalá, Madrid, Spain.
Gutierrez-Ocaña
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Perez-Albarsanz
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Recio
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D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D004161:Diphenylhexatriene; D005454:Fluorescence Polarization; D001556:Hexachlorocyclohexane / Q000494:pharmacology*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects* / Q000502:physiology; D010712:Phosphatidic Acids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013109:Sphingomyelins / Q000378:metabolism
D000085:Acetates; D010712:Phosphatidic Acids; D010743:Phospholipids; D013109:Sphingomyelins; D004161:Diphenylhexatriene; D001556:Hexachlorocyclohexane; D002784:Cholesterol; D019342:Acetic Acid