An improved method of vital staining of the corneal endothelium.
We present an improved and simplified method for vital staining of the corneal endothelium, which allows high-quality images that are excellent for morphological and morphometric studies. This method is based upon the combination of trypan blue and alizarin red S. The main advantages of this technique are simplicity, rapidity and low cost.
Ruíz
J M
JM
Division of Ophthalmology, Faculty of Medicine, University of Alicante, Spain.
Medrano
M
M
Alió
J L
JL
D000818:Animals; D000880:Anthraquinones; D002415:Cats; D004728:Endothelium, Corneal / Q000166:cytology*; D006168:Guinea Pigs; D006801:Humans; D008853:Microscopy; D011817:Rabbits; D013194:Staining and Labeling / Q000379:methods; D014343:Trypan Blue
D000880:Anthraquinones; C004468:Alizarin Red S; D014343:Trypan Blue