IFN-gamma enhances the expression of cell surface receptors for monoclonal nonspecific suppressor factor.
Monoclonal nonspecific suppressor factor (MNSF) is a lymphokine derived from a murine T cell hybridoma. The action of MNSF is mediated by specific cell-surface receptors. Since IFN-gamma alters the cellular response to MNSF (M. Nakamura, H. Ogawa, and T. Tsunematsu, J. Immunol. 138, 1799, 1987), we investigated whether IFN-gamma has an effect on the expression of MNSF receptor on target cells. IFN-gamma enhanced the expression of MNSF receptor on both MOPC-31C cells (a murine plasmacytoma line) and EL4 (a murine T lymphoma line). Incubation with IFN-gamma increased the number of specific MNSF-binding sites by about 50 to 90%, with no significant change in binding affinity. IFN-alpha and IFN-beta also increased MNSF binding, although the effect of the saturating amounts was lower than that seen with IFN-gamma. Maximal enhancement of receptor expression was observed after about 15 hr of incubation with IFN-gamma. No demonstrable change occurred in the kinetics of internalization of 125I-MNSF bound to MOPC-31C cells preincubated without or with IFN-gamma.
Nakamura
M
M
Third Division of Internal Medicine, Shimane Medical University, Izumo, Japan.
Ogawa
H
H
Tsunematsu
T
T
D000818:Animals; D004705:Endocytosis; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000494:pharmacology; D016899:Interferon-beta / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic / Q000502:physiology*; D011994:Recombinant Proteins; D013491:Suppressor Factors, Immunologic / Q000502:physiology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D016898:Interferon-alpha; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D013491:Suppressor Factors, Immunologic; C066109:monoclonal-nonspecific suppressor factor receptor; D016899:Interferon-beta; D007371:Interferon-gamma