title: Localization of hepatitis C virus (HCV) antigen by immunohistochemistry on fixed-embedded liver tissue.
abstract: An immunohistochemical method of staining HCV-related antigens in fixed-embedded liver biopsies is described. Two primary antisera were used: 1) a high titre anti-HCV human IgG separated from an anti-HCV positive serum; and 2) rabbit anti-HCV antibodies. In our experience this method proves to be reproducible and shows a good correlation with serologic results.
authors.affiliation: Servizio di Anatomia ed Istologia, Ospedale di Castelfranco Veneto, Torino, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Infantolino
authors.forename: F
authors.initials: F
authors.lastname: Bonino
authors.forename: A R
authors.initials: AR
authors.lastname: Zanetti
authors.forename: R R
authors.initials: RR
authors.lastname: Lesniewski
authors.forename: R
authors.initials: R
authors.lastname: Barbazza
authors.forename: M
authors.initials: M
authors.lastname: Chiaramonte
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification*; D001706:Biopsy; D005404:Fixatives; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000008:administration & dosage; D006526:Hepatitis C / Q000276:immunology / Q000473:pathology; D018936:Hepatitis C Antigens; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000276:immunology / Q000473:pathology*; D013194:Staining and Labeling; D016610:Tissue Embedding
chemical_list: D000956:Antigens, Viral; D005404:Fixatives; D006508:Hepatitis Antibodies; D018936:Hepatitis C Antigens