title: Changes in 7SL RNA conformation during the signal recognition particle cycle.
abstract: The structure of 7SL RNA has been probed by chemical modification followed by primer extension, using four substrates: (i) naked 7SL RNA; (ii) free signal recognition particle (SRP); (iii) polysome bound SRP; and (iv) membrane bound SRP. Decreasing sensitivity to chemical modification between these different substrates suggests regions on 7SL RNA that: bind proteins associated with SRP might interact with ribosomes; and are protected by binding to membranes. Other areas increase in chemical sensitivity, exemplified by a tertiary interaction present in naked 7SL RNA but not in free SRP. Such changes suggest that 7SL RNA changes its conformation during the SRP cycle. These conformational changes could be a necessary component to move through the SRP cycle from one stage to the next.
authors.affiliation: Brown University Division of Biology and Medicine, Providence, RI 02912.
authors.forename: M
authors.initials: M
authors.lastname: Andreazzoli
authors.forename: S A
authors.initials: SA
authors.lastname: Gerbi
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004285:Dogs; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes; D010179:Pancreas / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D018271:Signal Recognition Particle
chemical_list: D015345:Oligonucleotide Probes; D012261:Ribonucleoproteins; D018271:Signal Recognition Particle; D012313:RNA