Alterations of the intestinal innervation in mice infected with Schistosoma mansoni.
Schistosomiasis mansoni is a parasitic disease in which granulomas form around schistosome eggs in the liver and intestines. The purpose of this study was to determine the alterations in the intrinsic innervation of the distal ileum and proximal colon resulting from schistosomiasis. Using murine schistosomiasis mansoni, we examined light microscopic preparations stained with osmium-zinc iodide or the dihydronicotinamide adenine dinucleotide: nitro BT oxidoreductase (NADH) method. We also examined specific populations of peptidergic nerves (vasoactive intestinal polypeptide and substance P) using an avidin-biotin complex (ABC) immunohistochemical technique. We found that granulomas focally destroyed the enteric nerves. Occasionally nerves were found within granulomas, particularly at the periphery of the lesions. Nerve cell bodies close to granulomas had altered staining, which included increased staining for vasoactive intestinal polypeptide. The distribution of nerve injury varied between the 2 enteric segments studied. In the distal ileum, the principal injury was to the myenteric plexus; whereas, the submucous and mucosal plexuses were predominantly damaged in the proximal colon. The physiologic significance of this injury to the enteric nerves requires elucidation.
Varilek
G W
GW
Department of Internal Medicine, University of Iowa, Iowa City 52242.
Weinstock
J V
JV
Williams
T H
TH
Jew
J
J
D000818:Animals; D003106:Colon / Q000294:innervation*; D005260:Female; D006099:Granuloma / Q000473:pathology*; D007082:Ileum / Q000294:innervation*; D007150:Immunohistochemistry; D051379:Mice; D008808:Mice, Inbred CBA; D012555:Schistosomiasis mansoni / Q000473:pathology*; D013373:Substance P / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D013373:Substance P; D014660:Vasoactive Intestinal Peptide