Relationship between DNA methylation and cell proliferation in Trypanosoma cruzi.
5-Azacytidine treatment of T. cruzi epimastigotes in culture induces active cell proliferation. This effect was detected as an increase in the cell number and [3H-methyl]thymidine incorporation into DNA. 5-Azacytidine does not alter other metabolic parameters. We have previously demonstrated that 5-azacytidine induces DNA hypomethylation in T. cruzi. Accordingly, we suggest that this chemical modification may be related to the control of T. cruzi cell division.
Rojas
M V
MV
Department of Cell Biology and Genetics, School of Medicine, University of Chile, Santiago.
Galanti
N
N
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA; D004261:DNA Replication / Q000187:drug effects; D016054:DNA, Protozoan / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008745:Methylation; D010101:Oxygen Consumption / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
D016054:DNA, Protozoan; D004247:DNA; D001374:Azacitidine; D013936:Thymidine