A possible contribution of mRNA secondary structure to translation initiation efficiency in Lactococcus lactis.
Gene expression signals derived from Lactococcus lactis were linked to lacZ-fused genes with different 5'-nucleotide sequences. Computer predictions of mRNA secondary structure were combined with lacZ expression studies to direct base-substitutions that could possibly influence gene expression. Mutations were made such that the DNA sequence upstream of the ATG start codon was not changed. Moreover, care was taken that the substitutions, which were all within the first six codons, neither affected the amino acid sequence of the gene product nor introduced codons rarely used in L. lactis. The results suggest that mRNA secondary structure contributes to the efficiency of translation initiation in L. lactis.
van de Guchte
M
M
Department of Genetics, Centre of Biological Sciences, University of Groningen, Haren, The Netherlands.
van der Lende
T
T
Kok
J
J
Venema
G
G
D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D012329:RNA, Bacterial; D012333:RNA, Messenger; D001616:beta-Galactosidase