Plasmid DNAs directly injected into mouse brain with lipofectin can be incorporated and expressed by brain cells.
In this study, we demonstrated that lipofectin-treated DNAs which were injected into mouse brain could be incorporated and expressed by brain cells. When L7RH-beta gal plasmid DNA harboring E. coli beta-galactosidase gene fused with the nuclear location signal of SV40 T-antigen gene was injected into brains of 1-week-old mice, cells whose nuclei appeared to be densely stained with the chromogenic substrate X-gal were detected in several portions of the brain till 9 days after injection. Injection of pMLV-CAT plasmid DNA which contains the E. coli chloramphenicol acetyltransferase (CAT) gene also resulted in cells immunoreactive to the anti-CAT antibody.
Ono
T
T
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Fujino
Y
Y
Tsuchiya
T
T
Tsuda
M
M
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006651:Histocytochemistry; D051379:Mice; D008845:Microinjections; D010714:Phosphatidylethanolamines / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics*; D013194:Staining and Labeling; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase