Interaction between organic phosphates and sheep hemoglobins.
Measurements have been made of the effect of the organic phosphate 2,3-DPG and inositol hexaphosphate (IP6) on the OEC of sheep hemoglobins A, B, and F. A thin film method (HEM-O-SCAN) was used and the hemoglobin concentration was about one quarter to one third of that normally found in red cells. All experiments were in bis-tris-saline buffer and in the absence of CO2. At low concentrations of IP6 and at concentrations of 2,3-DPG similar to red cell concentrations, the OEC was right-shifted in all cases, P50 being increased by a factor of 1.58-1.84 by IP6 and by a factor of 1.41-1.59 by 2,3-DPG. The value of Hill n, the index of cooperativity was not generally changed by 2,3-DPG but was decreased by IP6.
Holland
R A
RA
School of Physiology and Pharmacology, University of New South Wales, Kensington, Australia.
Tibben
E A
EA
Hallam
J F
JF
D019794:2,3-Diphosphoglycerate; D000818:Animals; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D005319:Fetal Hemoglobin / Q000378:metabolism; D006441:Hemoglobin A / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D066298:In Vitro Techniques; D010100:Oxygen / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism; D012756:Sheep
D004163:Diphosphoglyceric Acids; D006454:Hemoglobins; D010710:Phosphates; D019794:2,3-Diphosphoglycerate; D010833:Phytic Acid; D006441:Hemoglobin A; D005319:Fetal Hemoglobin; C020622:hemoglobin B; D010100:Oxygen