title: Epitope recognition of conserved HIV envelope sequences by human cytotoxic T lymphocytes.
abstract: CTL constitute an essential part of the immune response against the HIV. CTL recognize peptides derived from viral proteins together with the MHC class I molecules on the surface of infected cells. The CTL response could be important in prevention or control of infection with HIV by destroying virus-producing cells. In this study we have attempted to identify peptide epitopes recognized by HIV-specific CTL. Using synthetic peptides, we have identified six conserved peptidic epitopes on the gp120 envelope glycoprotein recognized by polyclonal human CTL in association with HLA-A2 class I transplantation Ag. These results were confirmed by two approaches: i) blocking of CTL activities with antibodies specific for three of these conserved peptides; and ii) construction of doubly transfected P815-A2 target cells, using deletions of the HIV env gene. Vaccination or immunotherapy in HLA-A2 individuals can thus be considered using highly conserved HIV env peptidic sequences.
authors.affiliation: Laboratoire de Biologie et Immunologie Moléculaires des Rétrovirus, Institut Pasteur, Paris, France.
authors.forename: G
authors.initials: G
authors.lastname: Dadaglio
authors.forename: A
authors.initials: A
authors.lastname: Leroux
authors.forename: P
authors.initials: P
authors.lastname: Langlade-Demoyen
authors.forename: E M
authors.initials: EM
authors.lastname: Bahraoui
authors.forename: F
authors.initials: F
authors.lastname: Traincard
authors.forename: R
authors.initials: R
authors.lastname: Fisher
authors.forename: F
authors.initials: F
authors.lastname: Plata
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D000939:Epitopes / Q000032:analysis*; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000276:immunology; D015235:HLA-B Antigens / Q000276:immunology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection
chemical_list: D000939:Epitopes; D015699:HIV Envelope Protein gp120; D015789:HLA-A2 Antigen; D015235:HLA-B Antigens; D010446:Peptide Fragments