Separation of interstitial and tubular cells of human testis by means of an anti-substance P-antiserum. Androstane-3 beta, 17 beta-diol is exclusively formed by seminiferous tubules.
Leydig cells and seminiferous tubules of human testicular tissue were successfully separated by means of an anti-substance P-antiserum. On incubation with (14C)-testosterone or (14C)-dihydrotestosterone (DHT) it could be shown that besides DHT and 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha-diol) also 5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol) originated in the tubular compartment. The ratio 3 alpha/3 beta-diol was found to be higher (0.7) here compared to incubation with whole testicular tissue (0.2), indicating that the testicular interstitium influences the metabolism of DHT by the seminiferous tubules.
Tamm
J
J
Department of Medicine, Institute of Anatomy, University of Hamburg, Federal Republic of Germany.
Volkwein
U
U
Schulze
W
W
D000368:Aged; D000369:Aged, 80 and over; D015113:Androstane-3,17-diol / Q000096:biosynthesis*; D002469:Cell Separation / Q000379:methods; D013196:Dihydrotestosterone / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000166:cytology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012671:Seminiferous Tubules / Q000166:cytology / Q000378:metabolism; D013237:Stereoisomerism; D013373:Substance P / Q000378:metabolism; D013737:Testis / Q000166:cytology* / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol; D013373:Substance P; D013739:Testosterone