Characterization of a human trypsin resistant to Kunitz soybean trypsin inhibitor. Studies of duodenal juices after tube instillation of raw soybean extract.
Human duodenal juices collected during tube instillation of raw soybean extract into the duodenum contained free trypsin and free Kuntiz soybean trypsin inhibitor (KTI) in the simultaneous presence of trypsin-KTI complexes. It has previously been suggested that this KTI-non-inhibitable trypsin has a general resistance to serine protease inhibitors. Four different trypsin forms have been found and partly characterized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and isoelectric focusing followed by Western immunoblotting or enzyme staining. In addition, crossed immunoelectrophoresis and affinity chromatography with antibody-coupled gels have been used for identification of free and inhibitor-complexed trypsin.
Thorsen
L I
LI
Matforsk, Norwegian Food Research Institute, Aas.
Holm
H
H
Reseland
J E
JE
Bjørnsen
S
S
Hanssen
L E
LE
Brosstad
F
F
D015153:Blotting, Western; D002846:Chromatography, Affinity; D004386:Duodenum / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007419:Intestinal Secretions / Q000737:chemistry*; D007525:Isoelectric Focusing; D013025:Glycine max; D013194:Staining and Labeling; D014357:Trypsin / Q000032:analysis* / Q000145:classification; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000032:analysis*
D014360:Trypsin Inhibitor, Kunitz Soybean; D014357:Trypsin