[Evaluation of culture media for detecting the starch hydrolysis reaction in pathovars of Xanthomonas campestris].
Sixty strains of different pathovars of Xanthomonas campestris have been tested for the evaluation of various starch agars and compounds of starch degradation on six media: soluble starch, potato insoluble starch, corn insoluble starch, potato amylopectin, corn amylopectin and potato amylose. The purpose of the present investigation was the selection of the most suitable medium for the visualization of the starch hydrolysis test, presenting this reaction as a distinct character between pathovars of the Xanthomonas campestris group. From 60 strains tested, 74% gave positive reactions. Pathovars holcicola, pelargonii, pruni and vitians were negative. Regarding X. campestris pv. vesicatoria cultures, results were variable. Potato and corn insoluble starch agars were the most suitable media for the visualization of the starch hydrolysis reaction and at the same time the most appropriate for direct isolation. Differentiation at species level could be practicable, but within the Xanthomonas campestris group, variation amongst pathovars suggest the unsuitability of the test in spite of the high percentage of positive reactions.
Alippi
A M
AM
Cátedra de Fitopatología, Facultad de Agronomía, Universidad Nacional de La Plata, Argentina.
D000681:Amylases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001431:Bacteriological Techniques; D003470:Culture Media; D006868:Hydrolysis; D013045:Species Specificity; D013194:Staining and Labeling; D013213:Starch / Q000378:metabolism*; D016959:Xanthomonas campestris / Q000145:classification* / Q000201:enzymology / Q000254:growth & development
D001426:Bacterial Proteins; D003470:Culture Media; D013213:Starch; D000681:Amylases
Evaluación de medios de cultivo para detectar la reacción de hidrólisis del almidón por patovares de Xanthomonas campestris.