Purification of neuropathy target esterase from avian brain after prelabelling with [3H]diisopropyl phosphorofluoridate.
Neuropathy target esterase from hen brains was radiolabelled at the active site with [3H]diisopropyl phosphorofluoridate. The labelled protein was purified by differential centrifugation and Nonidet P40 solubilization, detergent phase partitioning, anion exchange, and preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The volatilizable counts assay and analytical SDS-PAGE were used to monitor the protein. The 150-kDa subunit polypeptide appears as a single band on analytical SDS-PAGE.
Rüffer-Turner
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ME
MRC Toxicology Unit, Medical Research Council Laboratories, Surrey, England, U.K.
Read
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DJ
Johnson
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MK
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification*; D002498:Centrifugation; D002645:Chickens; D002852:Chromatography, Ion Exchange; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D007531:Isoflurophate; D017830:Octoxynol; D011092:Polyethylene Glycols; D012995:Solubility; D014316:Tritium
D003902:Detergents; D014316:Tritium; D007531:Isoflurophate; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D002265:Carboxylic Ester Hydrolases; C021859:neurotoxic esterase