Automated procedure for dewaxing and rehydration of paraffin-embedded tissue sections for DNA flow cytometric analysis of breast tumors.
Flow cytometric DNA analysis of paraffin-embedded tumors is an important diagnostic and prognostic tool in clinical pathology. The technique is limited, however, by the time-consuming multistep procedure for dewaxing and rehydrating tissue. The authors developed an automated procedure to complete the dewaxing and rehydration of tissue using a routine histologic tissue processor with a 24-hour timer. This technique provided excellent tissue recovery and reproducible DNA histograms comparable to those obtained by manual methods. Subsequently, the authors analyzed the DNA content of 93 paraffin-embedded breast cancer tissues. The automation of a significant portion of the routine processing required for paraffin-embedded tissue makes cytometric DNA analysis a more practical procedure in the laboratory.
Babiak
J
J
Department of Laboratory Medicine, Cross Cancer Institute, Edmonton, Alberta, Canada.
Poppema
S
S
D001331:Automation; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004247:DNA / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006652:Histological Techniques / Q000295:instrumentation; D006801:Humans; D007399:Interphase; D010232:Paraffin; D011003:Ploidies; D013194:Staining and Labeling; D014867:Water
D014867:Water; D010232:Paraffin; D004247:DNA