Interleukin 4 down-regulates the expression of CD14 and the production of interleukin 6 in acute myeloid leukemia cells.
On normal monocytes, interleukin 4 (IL-4) down-regulates the production of several proteins that are produced in response to bacterial cell wall lipopolysaccharides (LPS), among others IL-6. In addition, IL-4 was shown to down-regulate the expression of the monocytic differentiation marker CD14, recently identified as a receptor for LPS. Since (autocrine) IL-6 is possibly involved in the proliferation and differentiation of acute myeloid leukemia cells with myelomonocytic differentiation (AML-M4/M5), we studied the effects of IL-4 on IL-6 production and CD14 expression by these cells. We found that IL-4 down-regulates both the expression of CD14 (n = 6) and the production of IL-6 (n = 9) in AML cells. Using Northern blot analysis, we found that IL-4 down-regulates the level of steady-state mRNA for both CD14 (two of three cases tested) and IL-6 (three cases tested).
Jansen
J H
JH
Laboratory of Experimental Hematology, University Medical Center, Leiden, The Netherlands.
Fibbe
W E
WE
Wientjens
G J
GJ
Van Damme
J
J
Landegent
J E
JE
Willemze
R
R
Kluin-Nelemans
J C
JC
D000208:Acute Disease; D015703:Antigens, CD / Q000378:metabolism*; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism*; D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000502:physiology; D015536:Down-Regulation / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics / Q000502:physiology*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007951:Leukemia, Myeloid / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015850:Interleukin-6; D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger; D015847:Interleukin-4