Protection of immunosuppressed mice against infection with Pseudomonas aeruginosa by recombinant P. aeruginosa lipoprotein I and lipoprotein I-specific monoclonal antibodies.
Outer membrane protein I (OprI) is one of the major proteins of the outer membrane of Pseudomonas aeruginosa. The protective effect of OprI vaccination and that of three OprI-specific monoclonal antibodies (MAbs) against infection with P. aeruginosa were tested in immunosuppressed mice. The combination of Oprl and MAb 2A1 protected the mice against a challenge with a 96-fold 50% lethal dose. The binding site of MAb 2A1 was mapped, resulting in the identification of a protective epitope (amino acids 7 to 20).
Finke
M
M
Chirurgische Universitätsklinik, Chirurgische Forschu, Freiburg im Breigau, Federal Republic of Germany.
Muth
G
G
Reichhelm
T
T
Thoma
M
M
Duchêne
M
M
Hungerer
K D
KD
Domdey
H
H
von Specht
B U
BU
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D007108:Immune Tolerance; D007114:Immunization; D007116:Immunization, Passive; D008074:Lipoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011552:Pseudomonas Infections / Q000276:immunology / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D000939:Epitopes; D008074:Lipoproteins; D011994:Recombinant Proteins; C060507:outer membrane lipoprotein I, Bacteria