title: Avian leucocyte common antigens: molecular weight determination and flow cytometric analysis using new monoclonal antibodies.
abstract: New leucocyte common antigens expressed on all the normal chicken leucocytes have been characterized using two monoclonal antibodies designated as K-11 and K-55. These monoclonal antibodies stained virtually 100% of the leucocytes derived from various lymphoid organs including the spleen, thymus, bursa of Fabricius, caecal tonsil and peripheral blood, as well as a monocytic cell line (MC29), a B cell line (LSCC-RP9), and a T cell line (CU12). However, they did not stain mature erythrocytes, intestinal epithelial cells, or chicken embryonic fibroblasts. The two monoclonal antibodies showed different staining patterns and detected non-overlapping epitopes on MC29 cells in two color immunofluorescence analysis. Western blot analysis under non-reducing conditions showed that the monoclonal antibody K-11 recognized three splenic leucocyte proteins with molecular weights of 92, 42 and 41 kDa, whereas the monoclonal antibody K-55 recognized two proteins with molecular weights of 97 and 42 kDa. The data indicate that the monoclonal antibodies K-11 and K-55 recognize novel leucocyte-common antigens which have lower molecular weights than the previously reported leucocyte-common antigen family.
authors.affiliation: Livestock and Poultry Sciences Institute, U.S. Department of Agriculture, ARS, Beltsville, MD 20705.
authors.forename: K S
authors.initials: KS
authors.lastname: Chung
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: M C
authors.initials: MC
authors.lastname: Jenkins
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D002645:Chickens / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006649:Histocompatibility Antigens / Q000276:immunology*; D017493:Leukocyte Common Antigens; D008221:Lymphoid Tissue / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010749:Phosphoprotein Phosphatases / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000939:Epitopes; D006649:Histocompatibility Antigens; D008562:Membrane Glycoproteins; D010749:Phosphoprotein Phosphatases; D017493:Leukocyte Common Antigens