title: Affinity purification of human tau proteins and the construction of a sensitive sandwich enzyme-linked immunosorbent assay for human tau detection.
abstract: Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau protein was used to purify tau proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau proteins. The affinity-purified human tau was used to produce a high-titered rabbit anti-human tau serum. The monoclonal anti-tau antibody and the polyclonal rabbit anti-tau serum were then used to construct a sandwich enzyme-linked immunosorbent assay for detection of human tau proteins, with a sensitivity of 1 ng/ml.
authors.affiliation: Innogenetics, Ghent, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Mercken
authors.forename: M
authors.initials: M
authors.lastname: Vandermeeren
authors.forename: U
authors.initials: U
authors.lastname: Lübke
authors.forename: J
authors.initials: J
authors.lastname: Six
authors.forename: J
authors.initials: J
authors.lastname: Boons
authors.forename: E
authors.initials: E
authors.lastname: Vanmechelen
authors.forename: A
authors.initials: A
authors.lastname: Van de Voorde
authors.forename: J
authors.initials: J
authors.lastname: Gheuens
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007106:Immune Sera; D016875:tau Proteins / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D007106:Immune Sera; D016875:tau Proteins