[Determination of tuberculosis antibodies using an immunoenzyme assay in lyophilized samples of capillary blood].
The aim of this work was to find the technique of enzyme immunoassay (EIA) of capillary blood lyophilized on a filter paper disk. The author has selected a buffer on the basis of egg yolk aqueous extract, that permits blocking of nonspecific binding in EIA but not preventing the specific antigen-antibody interactions. The results of analyses of capillary blood lyophilized drops and of blood serum samples, carried out in 7 tuberculous patients and 6 donors, coincided. The results were tested in 57 blood samples.
Vishnevskaia
E B
EB
D000907:Antibodies, Bacterial / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
D000907:Antibodies, Bacterial
Opredelenie protivotuberkuleznykh antitel metodom immunofermentnogo analiza v sukhikh obraztsakh kapilliarnoĭ krovi.