Distribution and effect of galanin on gallbladder and sphincter of Oddi motility in the pig.
This study was designed to determine the occurrence and topographical distribution of galanin-like immunoreactivity (GAL-LI) in the porcine gallbladder and sphincter of Oddi and to investigate the pharmacologic effect of GAL on gallbladder and sphincter of Oddi motility. By radioimmunoassay the concentration of GAL-LI in the gallbladder was 2.75 +/- 0.23, 9.73 +/- 1.33 in the common bile duct and 5.10 +/- 0.37 in the sphincter of Oddi (pmol/g +/- SE). By immunohistochemistry GAL-LI was found exclusively in ganglionic cells and in nerve fibers among the smooth muscle bundles. Gallbladder and sphincter of Oddi pressures were recorded before and during 5-minute local intraarterial infusion of 4, 8, 19, 39, 78 and 194 ng GAL - Kg-1 - min-1 in 12 anaesthetized pigs. GAL in doses greater than or equal to 39 ng.kg-1.min-1 significantly reduced sphincter of Oddi phasic wave frequency (4.8 +/- 0.4 vs. 2.1 +/- 0.5; p = 0.004) and sphincter of Oddi motility index (70.2 +/- 6.02 vs. 27.7 +/- 8.3; p = 0.002) but did not affect gallbladder pressure. We conclude that the distribution of GAL-LI in the sphincter of Oddi and the effect that a pharmacologic dose of GAL has on sphincter of Oddi motor activity, suggests that GAL may be involved in the physiologic control of bile flow in the pig.
Harling
H
H
Department of Surgical Gastroenterology C, Rigshospitalet, University of Copenhagen, Denmark.
Messell
T
T
Jensen
S L
SL
Poulsen
S S
SS
D000818:Animals; D001646:Bile / Q000502:physiology*; D019004:Galanin; D005704:Gallbladder / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D007150:Immunohistochemistry; D009479:Neuropeptides / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D011863:Radioimmunoassay; D009803:Sphincter of Oddi / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D013552:Swine
D009479:Neuropeptides; D010455:Peptides; D019004:Galanin