Evidence for an involvement of eicosanoids in neurokinin3-receptor mediated acetylcholine release from myenteric neurons.
The release of acetylcholine (ACh) from myenteric plexus evoked by 5-hydroxytryptamine (5-HT) and senktide (a selective neurokinin3 (NK3) agonist) was depressed by mepacrine, an inhibitor for phospholipase A2 activity. Release of ACh was stimulated by arachidonic acid; this release was partially depressed by nordihydroguaiaretic acid (NDGA), which inhibits lipoxygenase activity. NDGA failed to modify the ACh secretion elicited by 5-HT. Release of ACh evoked by senktide was significantly inhibited by NDGA, suggesting an involvement of eicosanoids in the release of ACh elicited by specific neurokinin receptors in myenteric neurons.
Yau
W M
WM
Department of Physiology, School of Medicine, Southern Illinois University, Carbondale 62901-6512.
Bowen
D J
DJ
Youther
M L
ML
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D015777:Eicosanoids / Q000502:physiology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009637:Masoprocol / Q000494:pharmacology; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism; D009197:Myenteric Plexus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D011796:Quinacrine / Q000494:pharmacology; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000378:metabolism / Q000502:physiology; D013373:Substance P / Q000031:analogs & derivatives / Q000494:pharmacology
D015777:Eicosanoids; D010446:Peptide Fragments; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter; D011985:Receptors, Serotonin; C054724:senktide; D016718:Arachidonic Acid; D013373:Substance P; D009637:Masoprocol; D010741:Phospholipases A; D054467:Phospholipases A2; D011796:Quinacrine; D000109:Acetylcholine