Electrophoretic pattern of rodent seminal vesicle proteins as revealed by silver staining.
Electrophoresis of seminal vesicle secretions (SVS) from several rodents showed a very simple pattern composed of 3-5 main protein bands when an anionic dye (Coomassie brilliant blue) was used. However, use of a silver staining method showed a more complex protein spectrum, and several minor components of 12-90 kD, were clearly revealed. Western blotting using antibodies to SVS demonstrated that these minor protein components were not serum contaminants. Rat, mouse and hamster SVS shared antigenic determinants which were not related to rabbit SVS.
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Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
Esponda
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D000818:Animals; D015153:Blotting, Western; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008647:Mesocricetus; D051379:Mice; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012394:Rosaniline Dyes; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000737:chemistry* / Q000378:metabolism; D012834:Silver; D013194:Staining and Labeling
D029584:Prostatic Secretory Proteins; D011506:Proteins; D012394:Rosaniline Dyes; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D012834:Silver; C048139:Coomassie blue