Zinc depletion modifies CD5 expression by 70Z/3 murine pre-B leukemia cell line.
CD5 expression on B cells is regulated by certain humoral factors. In a pre-B leukemia cell line 70Z/3, we found that interleukin 4 down-regulates it. Herein, we report that zinc influences spontaneous CD5 expression by this cell line as well as actions of these factors on CD5 expression considerably. In zinc-depleted culture media, spontaneous CD5 expression by 70Z/3 cells was enhanced. In contrast, the down-regulatory action of interleukin 4 was significantly reduced under culture conditions of zinc depletion. The supplementation of zinc to physiologic concentrations (1 to 2 microM) abolished such effects of zinc-depleted medium. The reduction of the suppressive action of interleukin 4 was observed at the level of gene expression. However, CD5 mRNA expression enhanced by lipopolysaccharide or NZB-SF was not further enhanced under conditions of zinc deficiency. These observations may suggest that CD5 expression by malignant or even normal B cells may be influenced by cellular/serum zinc levels.
Jyonouchi
H
H
Department of Pediatrics/St. Petersburg, University of South Florida College of Medicine/All Children's Hospital 33701.
Voss
R M
RM
Good
R A
RA
D000818:Animals; D015703:Antigens, CD / Q000032:analysis* / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology; D018956:CD5 Antigens; D015847:Interleukin-4 / Q000494:pharmacology; D015448:Leukemia, B-Cell / Q000276:immunology*; D051379:Mice; D011289:Preleukemia / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000172:deficiency / Q000502:physiology*
D015703:Antigens, CD; D018956:CD5 Antigens; D012333:RNA, Messenger; D015847:Interleukin-4; D015032:Zinc