Physico-chemical analysis of resorcin-fuchsin reagents and its relevance to staining quality of elastic fibres.
Three Weigert's resorcin-fuchsin reagents for elastic fibres were prepared by using different lots of basic fucsin and by preparing the staining solutions with ferric chloride or ferric perchlorate. The solutions were analyzed by spectrophotometry, thin layer chromatography (TLC) and spectrofluorimetry. Their ability to stain elastic fibres was tested on several human tissue. The strongest stain and faintest background were obtained with the fuchsin-chloride reagent, which showed the most significant reduction of the absorption at 575 nm by spectrofluorimetry, associated with an increased absorption at 510 nm with respect to the other stains. The results showed that physico-chemical analysis can represent a reliable, although empirical, way to predict the usefulness of a resorcin-fuchsin reagent for histological work.
Crescenzi
A
A
Dipart. di Biopat. Umana, Università La Sapienza, Roma, Italy.
Muda
A O
AO
Origgi
P
P
Bonfichi
R
R
Faraggiana
T
T
D002855:Chromatography, Thin Layer; D004547:Elastic Tissue / Q000033:anatomy & histology*; D006801:Humans; D012118:Resorcinols / Q000737:chemistry*; D012394:Rosaniline Dyes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling / Q000379:methods*
D012118:Resorcinols; D012394:Rosaniline Dyes; C031389:resorcinol