title: Expression of SV40-lacZ gene in mouse preimplantation embryos after pronuclear microinjection.
abstract: In order to study the expression of an exogenous gene in developing mouse embryos during the preimplantation period, DNA carrying the SV40 early promoter fused with the Escherichia coli beta-galactosidase gene (lacZ) was microinjected into the pronucleus of fertilized mouse eggs. Expression of lacZ gene was detected by staining embryos with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) as a substrate at pH 7.2. The embryos expressing the lacZ gene showed various intensities of blue staining, all showing a mosaic pattern. The exogenous gene was expressed from the 4-cell stage until the blastocyst stage. The proportion of embryos expressing the lacZ gene was maximal (38%) at the morula stage, and the expression was dependent on the presence of the SV40 promoter.
authors.affiliation: Department of Animal Pathology, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takeda
authors.forename: Y
authors.initials: Y
authors.lastname: Toyoda
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D004247:DNA / Q000032:analysis*; D015971:Gene Expression Regulation, Enzymologic; D006863:Hydrogen-Ion Concentration; D007763:Lac Operon; D051379:Mice; D008845:Microinjections; D009028:Morula / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics; D013194:Staining and Labeling; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D004247:DNA; D001616:beta-Galactosidase