Characterization of amino acid aminotransferases of Methanococcus aeolicus.
Four aminotransferases were identified and characterized from Methanococcus aeolicus. Branched-chain aminotransferase (BcAT, EC 2.6.1.42), aspartate aminotransferase (AspAT, EC 2.6.1.1), and two aromatic aminotransferases (EC 2.6.1.57) were partially purified 175-, 84-, 600-, and 30-fold, respectively. The apparent molecular weight, substrate specificity, and kinetic properties of the BcAT were similar to those of other microbial BcATs. The AspAT had an apparent molecular weight of 162,000, which was unusually high. It had also a broad substrate specificity, which included activity towards alanine, a property which resembled the enzyme from Sulfolobus solfataricus. An additional alanine aminotransferase was not found in M. aeolicus, and this activity of AspAT could be physiologically significant. The apparent molecular weights of the aromatic aminotransferases (ArAT-I and ArAT-II) were 150,000 and 90,000, respectively. The methanococcal ArATs also had different pIs and kinetic constants. ArAT-I may be the major ArAT in methanococci. High concentrations of 2-ketoglutarate strongly inhibited valine, isoleucine, and alanine transaminations but were less inhibitory for leucine and aspartate transaminations. Aromatic amino acid transaminations were not inhibited by 2-ketoglutarate. 2-Ketoglutarate may play an important role in the regulation of amino acid biosynthesis in methanococci.
Xing
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Department of Microbiology, University of Georgia, Athens 30602.
Whitman
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WB
D000410:Alanine Transaminase / Q000737:chemistry; D000597:Amino Acids, Branched-Chain / Q000737:chemistry; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000737:chemistry; D001219:Aspartate Aminotransferases / Q000737:chemistry; D007525:Isoelectric Focusing; D017017:Methanococcus / Q000187:drug effects / Q000201:enzymology*; D008970:Molecular Weight; D000637:Transaminases / Q000737:chemistry* / Q000302:isolation & purification
D000597:Amino Acids, Branched-Chain; D000637:Transaminases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001142:Aromatic-L-Amino-Acid Decarboxylases