Bradyrhizobium japonicum nodD1 can be specifically induced by soybean flavonoids that do not induce the nodYABCSUIJ operon.
Besides genistein and daidzein, which are active inducers of the nodYABCSUIJ operon in Bradyrhizobium japonicum, soybean seeds also excrete compounds that are not inducers of the nodYABCSUIJ genes but enhance induction of this operon in the presence of a suboptimal genistein concentration. This synergism was studied in detail, and specific compounds were identified in seed exudate which specifically induce the nodD1 gene but not the nodYABCSUIJ operon. Therefore, our current hypothesis is that the observed synergism is caused by a specific induction of nodD1. The specific nodD1 inducers from soybean seed extract have been purified and characterized chemically. They appear to be derivatives of genistein, glycitein, and daidzein with glucose, malonyl, and acetyl groups attached. Both root and seed exudate appear to contain these compounds, with the seed being the major source. No hydrolysis of these compounds to their aglycone forms was detected in the presence of B. japonicum. A model for nod gene induction in B. japonicum is discussed.
Smit
G
G
Center for Legume Research, University of Tennessee, Knoxville 37996.
Puvanesarajah
V
V
Carlson
R W
RW
Barbour
W M
WM
Stacey
G
G
D001426:Bacterial Proteins / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000302:isolation & purification / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D019833:Genistein; D006868:Hydrolysis; D007529:Isoflavones / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008957:Models, Genetic; D009876:Operon; D012230:Rhizobiaceae / Q000235:genetics*; D013025:Glycine max / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet; D015533:Transcriptional Activation; D001616:beta-Galactosidase / Q000378:metabolism
D001426:Bacterial Proteins; D005419:Flavonoids; D007529:Isoflavones; C060773:NodD protein, Bacteria; C013908:daidzin; C086566:glycitein; D019833:Genistein; D001616:beta-Galactosidase