Antimutagenicity of propionic acid bacteria.
The antimutagenic effect of dialysed cell extracts of 4 strains of propionic acid bacteria was examined against the mutagenicity of sodium azide in the TA1535 tester strain of Salmonella typhimurium using the Ames test. It was noted that dialysates of 2 strains of Propionibacterium shermanii, P. pentosaceum and P. acnes, significantly reduced sodium azide-induced revertants. The dialysate of propionic acid cocci did not show an antimutagenic effect. The inhibitory activity was enhanced if the mutagen and extract were coincubated for 20 min prior to performing the mutagenicity assay. Antimutagenicity of dialysates from P. shermanii VKM-103 against MNNG and 9-aminoacridine was shown in S. typhimurium strains TA1535 and TA97. The antimutagenic activity was found in the protein fraction of the cell extract of P. shermanii. The proteins of the dialysate of P. shermanii were separated using a Toyopearl gel column into 3 main peaks according to their molecular weights. The antimutagenic activity towards sodium azide was found in the second and the third peaks. We suggest that dialysates of the cells of propionic acid bacteria contain several kinds of antimutagenic substances with different molecular weights.
Vorobjeva
L I
LI
Department of Microbiology, Biology Faculty, Moscow State University, U.S.S.R.
Cherdinceva
T A
TA
Abilev
S K
SK
Vorobjeva
N V
NV
D000585:Aminacrine / Q000633:toxicity; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001386:Azides / Q000037:antagonists & inhibitors* / Q000633:toxicity; D008769:Methylnitronitrosoguanidine / Q000037:antagonists & inhibitors / Q000633:toxicity; D009152:Mutagenicity Tests; D011422:Propionates / Q000494:pharmacology*; D011424:Propionibacterium / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000187:drug effects; D019810:Sodium Azide; D014805:Vitamin B 12 / Q000032:analysis
D016587:Antimutagenic Agents; D001386:Azides; D011422:Propionates; D008769:Methylnitronitrosoguanidine; D000585:Aminacrine; D019810:Sodium Azide; C029658:propionic acid; D014805:Vitamin B 12