title: Nucleic acid binding properties of the 92-kDa replicase subunit of alfalfa mosaic virus produced in yeast.
abstract: The 92-kDa non-structural protein of alfalfa mosaic virus (one of the replicase subunits) was synthesized by Saccharomyces cerevisiae transformed with a recombinant expression vector. The yeast-expressed protein had the immunological and size characteristics of the naturally made viral protein. It was partially purified and its nucleic acid binding properties were tested by gel-retardation electrophoresis and nitrocellulose adsorption. The protein interacted with single-stranded RNA, double-stranded RNA and double-stranded DNA in a salt-dependent manner, with a slight preference for RNA. These properties may be related to its putative function as a core RNA polymerase.
authors.affiliation: Institut de Biologie Moléculaire des Plantes du C.N.R.S., Université Louis Pasteur, Strasbourg, France.
authors.forename: C
authors.initials: C
authors.lastname: Erny
authors.forename: F
authors.initials: F
authors.lastname: Schoumacher
authors.forename: T
authors.initials: T
authors.lastname: Godefroy-Colburn
authors.forename: C
authors.initials: C
authors.lastname: Stussi-Garaud
mesh_terms: D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D000455:Medicago sativa / Q000382:microbiology*; D009029:Mosaic Viruses / Q000201:enzymology* / Q000235:genetics; D010957:Plasmids; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014170:Transformation, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins