Expression of the mRNA for the ligand of c-kit in mouse Sertoli cells.
The expression of the mRNA for SLF (the c-kit ligand), a product of the "steel" locus, has been investigated in postnatal mouse testis and homogeneous populations of testicular cells. The message was found expressed in postnatal mouse testis but not in germ cells. Studies on primary mouse Sertoli cell cultures from 18 day old mice show that Sertoli cells are the site of SLF mRNA expression in the seminiferous tubules. Treatment of Sertoli cell cultures with cAMP analogs led to a significant increase in the SLF mRNA levels.
Rossi
P
P
Department of Public Health and Cell Biology, II University of Rome Tor Vergata, Italy.
Albanesi
C
C
Grimaldi
P
P
Geremia
R
R
D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology; D015870:Gene Expression; D008024:Ligands; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019009:Proto-Oncogene Proteins c-kit; D012333:RNA, Messenger / Q000378:metabolism*; D012708:Sertoli Cells / Q000378:metabolism*; D013737:Testis / Q000378:metabolism
D008024:Ligands; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; D019009:Proto-Oncogene Proteins c-kit