Auto- and polyreactivity of IgM from CD5+ and CD5- cord blood B cells.
The presence of the CD5 (67 kDa) molecule on the surface of B cells has been considered a marker for cells producing auto- and polyreactive antibodies. Cord blood B lymphocytes (rich in CD5+ B cells) have been sorted into CD5 positive and negative populations by flow cytometry using monoclonal antibodies to CD20 and CD5. Clones of these populations were obtained by immortalization with Epstein-Barr virus. Clones derived from both CD5+ and CD5- B cells produced IgM which was auto- and polyreactive with a higher frequency of these specificities in the CD5+ population. These data indicate that expression of surface CD5 on cord blood B cells is not a definitive marker of an auto/polyreactive population.
Mackenzie
L E
LE
Department of Immunology, UCMSM, London, UK.
Youinou
P Y
PY
Hicks
R
R
Yuksel
B
B
Mageed
R A
RA
Lydyard
P M
PM
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology; D018956:CD5 Antigens; D002472:Cell Transformation, Viral / Q000276:immunology; D002999:Clone Cells; D004797:Enzyme-Linked Immunosorbent Assay; D005312:Fetal Blood / Q000276:immunology*; D005434:Flow Cytometry; D004854:Herpesvirus 4, Human / Q000254:growth & development; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis*; D016130:Immunophenotyping
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D001323:Autoantibodies; D018956:CD5 Antigens; D007075:Immunoglobulin M