Presence of substance P and angiotensin II in corticotropic cells of the lizard Gallotia galloti: immunochemical study in the adult and during ontogenesis.
The hypophysis of the lizard Gallotia galloti showed substance-P-like immunoreactivity in both the adenohypophysis (pars distalis, PD; pars intermedia, PI) and the neurohypophysis (median eminence and pars nervosa), whereas angiotensin-II-like immunoreactivity appeared only in PD and PI. The elution-restaining procedure has allowed us to demonstrate the colocalization of both peptides with adrenocorticotropic hormone (ACTH) in PD and PI cells. Electron microscopic study revealed the presence of substance P immunoreactivity on ACTH secretory granules. The ontogeny of both peptides in corticotropic cells has been studied, revealing that the presence of substance P in ACTH-containing cells of the PI occurs from the embryonic stage 33 (S 33), whereas in the PD it occurs from S 34, coinciding with the appearance of ACTH within the same cells. In both median eminence and pars nervosa of the neurohypophysis, substance P appeared later in development, at S 38. Angiotensin II immunoreactivity in PI cells first appeared at S 38, while in PD it appeared from S 40.
Bello
A R
AR
Departamento de Biologia Celular, Facultad de Biología, Universidad de La Laguna, Tenerife, España.
Marti
E
E
Lancha
A
A
Beauvillain
J C
JC
Tramu
G
G
Batista
M A
MA
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000804:Angiotensin II / Q000032:analysis*; D000818:Animals; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000378:metabolism; D007124:Immunoenzyme Techniques; D008116:Lizards / Q000196:embryology / Q000254:growth & development*; D008473:Median Eminence / Q000737:chemistry; D008854:Microscopy, Electron; D010902:Pituitary Gland / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure; D010903:Pituitary Gland, Anterior / Q000737:chemistry / Q000254:growth & development / Q000648:ultrastructure; D010904:Pituitary Gland, Posterior / Q000737:chemistry / Q000254:growth & development / Q000648:ultrastructure; D013373:Substance P / Q000032:analysis*
D000804:Angiotensin II; D013373:Substance P; D000324:Adrenocorticotropic Hormone