Induction of airway hyperresponsiveness in allergic rats.
Brown-Norway rats (male) were sensitized with both dinitrophenylated-bovine serum albumin (DNP-BSA) and Bordetella pertussis simultaneously in order to induce airway hyperresponsiveness (AHR) as the first sensitization. At five days, DNP-BSA was inhaled as a booster into the airways under thiopental anaesthesia. At eight days, inhalation of antigen markedly increased the tracheal pressure (TP) in sensitized rats (11.9 +/- 1.6 cmH2O) and slightly increased TP in non-sensitized rats (1.1 +/- 0.4), the difference between the two groups being significant (p less than 0.001). Twenty-four hours after antigen challenge, the airway responsiveness to ACh in sensitized rats was markedly increased to about 4-fold as compared to that in non-sensitized rats. Inhalation of dinitrophenylated-ovalbumin failed to increase the airway responsiveness to ACh in rats sensitized with DNP-BSA, although a marked increase in TP was induced immediately after antigen challenge. We thus succeeded in preparing a model of AHR by employing a new procedure of sensitization.
Shishikura
T
T
Life Science Research Laboratories, Showa Denko Co., Ohta-ku, Tokyo.
Yamada
M
M
Oguro
K
K
Tamaki
N
N
Kosugi
T
T
D000109:Acetylcholine / Q000494:pharmacology; D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000378:metabolism / Q000502:physiology; D001886:Bordetella pertussis / Q000502:physiology; D004140:Dinitrophenols / Q000008:administration & dosage / Q000378:metabolism; D000939:Epitopes; D051381:Rats; D011914:Rats, Inbred BN; D012130:Respiratory Hypersensitivity / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D012137:Respiratory System / Q000503:physiopathology; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000378:metabolism; D014132:Trachea / Q000187:drug effects / Q000502:physiology
D000941:Antigens; D004140:Dinitrophenols; D000939:Epitopes; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D000109:Acetylcholine