Extracellular ATP stimulates interleukin-dependent cultured mast cells and eosinophils through calcium mobilization.
We examined the effect of ATP and related nucleotides on the changes in intracellular calcium ([Ca2+]i) in murine bone marrow-derived mast cells (BMMC) and human cord blood-derived eosinophils (EO) cultured in the presence of interleukins. ATP, ADP and AMP released a substantial amount of histamine and leukotriene C4 from BMMC, and EO showed locomotive activity in response to ATP, ADP and GTP. These reactions were accompanied with an increase in [Ca2+]i in BMMC and in EO. The rise in [Ca2+]i in BMMC induced by ATP or antigen at optimal concentrations was inclined to be persisting. On the other hand, these nucleotides induced a rapid and transient rise in [Ca2+]i in EO. Purified human peripheral EO also exhibited locomotive activity and an increase in [Ca2+]i in response to ATP. These results indicate that extracellular ATP activates interleukin-dependent cultured mast cells and EO through Ca2+ mobilization, and suggest that ATP, which is known to be released from activated platelets or autonomic nerves, may stimulate in vivo counterparts of these cultured inflammatory cells.
Saito
H
H
Division of Allergy, National Children's Medical Research Center, Tokyo, Japan.
Ebisawa
M
M
Reason
D C
DC
Ohno
K
K
Kurihara
K
K
Sakaguchi
N
N
Ohgimi
A
A
Saito
E
E
Akasawa
A
A
Akimoto
K
K
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D007378:Interleukins / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D013189:SRS-A / Q000096:biosynthesis
D007378:Interleukins; D013189:SRS-A; D000255:Adenosine Triphosphate; D002118:Calcium