Preparation and characterization of monoclonal antibodies to substance P.
A series of mouse hybridomas that produced monoclonal antibodies reactive with Substance P was prepared in an attempt to find a surrogate NK-1 receptor. When the binding profile of one particular antibody, SubP14.36.1, was compared in a rank order plot with NK-1 receptors against various agonists and antagonists of Substance P, a high correlation was found between these two binding structures. In addition, this monoclonal antibody inhibited a functional assay for Substance P. This result indicated that the pharmacological effects of this peptide can be blocked by an antibody (Ab).
Frickey
P H
PH
Clinical Products Research Laboratory, Eastman Kodak Company, Rochester, New York 14650-2117.
Yanni
J M
JM
Appell
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D006168:Guinea Pigs; D006825:Hybridomas / Q000276:immunology; D007082:Ileum / Q000187:drug effects; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D011485:Protein Binding; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013373:Substance P / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter; D013373:Substance P