Analysis of rat brain microdialysate by gas chromatography-high-resolution selected-ion monitoring mass spectrometry.
Gas chromatography-high-resolution selected-ion monitoring mass spectrometry was used to analyze catecholamine metabolites in rat brain microdialysate. Dialysate samples were collected in vials containing stable isotope analogues of homovanillic acid (HVA), 3-methoxy-4-hydroxyphenylglycol (MHPG) and 5-hydroxyindoleacetic acid (5HIAA) and analyzed as their trimethylsilyl derivatives. The metabolite levels were monitored at 20-min intervals throughout the time course of the experiment, beginning immediately after surgery and implantation of the dialysis probe and ending 4 h after amphetamine treatment. The levels of HVA were observed to decrease after amphetamine treatment, while those of MHPG and 5HIAA did not change significantly.
Chang
S Y
SY
Burroughs Wellcome Co., Research Triangle Park, NC 27709.
Moore
T A
TA
Devaud
L L
LL
Taylor
L C
LC
Hollingsworth
E B
EB
D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002395:Catecholamines / Q000032:analysis*; D003956:Dialysis; D008401:Gas Chromatography-Mass Spectrometry; D006719:Homovanillic Acid / Q000032:analysis; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D002395:Catecholamines; D008734:Methoxyhydroxyphenylglycol; D006897:Hydroxyindoleacetic Acid; D000661:Amphetamine; D006719:Homovanillic Acid