Direct cleavage of a DNA fragment by a bleomycin-oligonucleotide derivative.
Bleomycin A5 oligonucleotide derivative was used for direct cleavage of a DNA target. In the presence of Fe2+ ions and 2-mercaptoethanol, Blm-R-pd(CCAAACA) (I) damaged the target. pd(TGTTTGGCGAAGGA), with the yield of 80% , without affecting its own oligonucleotide tail. The sites of cleavage were T3-T5 and G6-G7. Unbound bleomycin A5 damaged the G6-G7-C8 site. Reagent I formed more stable complementary complexes with the target than parent oligonucleotide (ATm = 11 C).
Sergeyev
D S
DS
Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
Godovikova
T S
TS
Zarytova
V F
VF
D001483:Base Sequence; D001761:Bleomycin / Q000494:pharmacology* / Q000031:analogs & derivatives; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D006868:Hydrolysis; D007501:Iron / Q000494:pharmacology; D008623:Mercaptoethanol / Q000494:pharmacology; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000494:pharmacology*
D009841:Oligonucleotides; D001761:Bleomycin; C025703:bleomycetin; D008623:Mercaptoethanol; D004247:DNA; D007501:Iron