title: Heterogeneous expression of CD23 epitopes by eosinophils from patients. Relationships with IgE-mediated functions.
abstract: The receptor for IgE (Fc epsilon RII) on human eosinophils presents some common characteristics with CD23, a differentiation marker of B cells. We have used flow cytometry for evaluating the expression of various epitopes of CD23 on purified eosinophils from patients with eosinophilia. A correlation was found between the binding of myeloma IgE protein and the binding of a monoclonal antibody (mAb 135), directed against the IgE-binding site of B cell CD23. Using two additional anti-CD23 mAb, directed (8-30) or not (3-5) against the IgE-binding site, a low expression of these CD23 epitopes was observed on eosinophils from different eosinophilic patients. Northern blot analysis of eosinophil RNA with the cDNA probe of CD23 revealed a low-abundance transcript in three of the six patients expressing membrane CD23. The inhibition by all anti-CD23 mAb of IgE-mediated cytotoxicity and IgE binding to eosinophils clearly indicated the participation of a CD23-related molecule in IgE-dependent eosinophil functions.
authors.affiliation: Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur de Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Capron
authors.forename: M J
authors.initials: MJ
authors.lastname: Truong
authors.forename: D
authors.initials: D
authors.lastname: Aldebert
authors.forename: V
authors.initials: V
authors.lastname: Gruart
authors.forename: M
authors.initials: M
authors.lastname: Suemura
authors.forename: G
authors.initials: G
authors.lastname: Delespesse
authors.forename: B
authors.initials: B
authors.lastname: Tourvieille
authors.forename: A
authors.initials: A
authors.lastname: Capron
mesh_terms: D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001667:Binding, Competitive; D003602:Cytotoxicity, Immunologic; D004804:Eosinophils / Q000276:immunology*; D000939:Epitopes; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology* / Q000378:metabolism; D066298:In Vitro Techniques; D012333:RNA, Messenger / Q000235:genetics; D011961:Receptors, Fc / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D017455:Receptors, IgE
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000939:Epitopes; D012333:RNA, Messenger; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E