title: Staining of nuclei in fungi by ethidium bromide.
abstract: Ethidium bromide, (0.1% solution in ethanol-water, 1:3, v/v) was used to stain nuclei in mycelia and spores of different fungi. Nuclei looked bright brick red under green excitation. This method is very efficient, specific, reproducible and cost-effective.
authors.affiliation: Department of Plant Pathology, G. B. Pant University of Agriculture and Technology, Pantnagar, India.
authors.forename: U S
authors.initials: US
authors.lastname: Singh
authors.forename: J
authors.initials: J
authors.lastname: Kumar
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D004271:DNA, Fungal / Q000378:metabolism; D004996:Ethidium; D005453:Fluorescence; D005658:Fungi / Q000378:metabolism / Q000648:ultrastructure*; D013172:Spores, Fungal; D013194:Staining and Labeling
chemical_list: D004271:DNA, Fungal; D004996:Ethidium