Two genes encoding the soybean translation elongation factor eEF-1 alpha are transcribed in seedling leaves.
A cDNA and a genomic DNA library from soybean (Glycine max L.) were used to identify and sequence two genes coding for the alpha-subunit of the translation elongation factor eEF-1. Within the coding part, the two genes (tefS1 and tefS2) diverge in 80 wobble positions thus yielding an identical protein composed of 447 amino acids. The soybean protein has about 95% similarity with eEF-1 alpha proteins of Arabidopsis thaliana and tomato. Both genes S1 and S2 contain, within the coding part at a site seemingly unique to higher plants, a single short intron of 86 and 116 nucleotides, respectively. The untranslated leader part of both genes is interrupted by a large intron (partially sequenced). Genes S1 and S2 are transcribed in young leaves. cDNA and gene-specific oligonucleotide probes interact with a unique transcript of close to 1.9 kb. Northern hybridization studies using RNAs from dark- and light-grown seedlings show that light sharply increases the level of stable transcripts (1.9 kb). A peak value is measured after about 3 h of illumination, afterwards the transcript concentration drops to about 10% of the peak value. Genes S1 and S2 follow a similar transcription pattern in developing seedling leaves, which is distinct from that of the rbcS genes measured in parallel experiments. According to northern results, S1 transcripts are more abundant in leaves at all measured stages of development than S2 transcripts.
Aguilar
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F
Laboratoire de Biochimie Végétale, Université de Neuchatel, Switzerland.
Montandon
P E
PE
Stutz
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E
D000595:Amino Acid Sequence; D001345:Autoradiography; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D005091:Exons; D015698:Genomic Library; D007438:Introns; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D012313:RNA / Q000302:isolation & purification; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics; D012639:Seeds / Q000378:metabolism; D013025:Glycine max / Q000235:genetics*; D014158:Transcription, Genetic
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010940:Plant Proteins; D021382:Protein Sorting Signals; D012313:RNA; D004247:DNA; D012273:Ribulose-Bisphosphate Carboxylase