DNA-dependent adenosinetriphosphatase A is the eukaryotic analogue of the bacteriophage T4 gene 44 protein: immunological identity of DNA replication-associated ATPases.
We report the construction of three stable murine hybridomas that secrete monoclonal antibodies which recognize calf thymus DNA-dependent adenosinetriphosphatase A. All three of the antibodies react specifically with calf thymus ATPase A and the gene 44 protein from the bacteriophage T4 DNA-dependent ATPase. Each of the three anti-ATPase A antibodies appears to recognize a different epitope and none of the antibodies inhibit DNA-dependent ATP hydrolysis by ATPase A. Furthermore, one of the antibodies has been shown to react with two different preparations of HeLa cell DNA-dependent ATPases and a yeast DNA-dependent ATPase, all of which have been implicated in the enzymology of DNA replication. These findings provide strong evidence for the role of ATPase A in DNA replication. These observations lead us to conclude that, apart from the nucleotide binding sites, there are at least three epitopes common to both the bacteriophage and eukaryotic DNA-dependent ATPases that we have examined and that the different preparations of the eukaryotic ATPases contain the same DNA-dependent ATPase.
Mesner
L D
LD
Department of Biochemistry, University of Virginia Health Sciences Center, Charlottesville 22908.
Sutherland
W M
WM
Hockensmith
J W
JW
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D004265:DNA Helicases; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis; D000939:Epitopes / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D013604:T-Phages / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D004271:DNA, Fungal; D000939:Epitopes; D014764:Viral Proteins; D000251:Adenosine Triphosphatases; D004265:DNA Helicases