Human ribosomal RNA gene repeats are localized in the dense fibrillar component of nucleoli: light and electron microscopic in situ hybridization in human Sertoli cells.
The distribution of the human ribosomal gene repeat within human Sertoli cell nucleoli was investigated with the help of DNA-DNA in situ hybridization at the light and electron microscopic level. Probes from both the transcribed part of the gene repeat and the "non-transcribed" spacer were found to hybridize predominantly to the dense fibrillar component of nucleoli. It therefore can be concluded that the dense fibrillar component of nucleoli is the major site of the intranucleolar location of the ribosomal DNA. This holds true not only for the dense fibrillar component adjacent to fibrillar centers, but also for the dense fibrillar component remote from the fibrillar centers.
Wachtler
F
F
Histologisch-Embryologisches Institut der Univ. Wien, Austria.
Schöfer
C
C
Mosgöller
W
W
Weipoltshammer
K
K
Schwarzacher
H G
HG
Guichaoua
M
M
Hartung
M
M
Stahl
A
A
Bergé-Lefranc
J L
JL
Gonzalez
I
I
D002466:Cell Nucleolus / Q000737:chemistry / Q000648:ultrastructure*; D004275:DNA, Ribosomal / Q000032:analysis*; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D015530:Nuclear Matrix / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012708:Sertoli Cells / Q000648:ultrastructure*
D004275:DNA, Ribosomal