Generation of monoclonal antibodies detecting specific epitopes in olfactory and respiratory epithelia.
Two panels of monoclonal antibodies have been generated, each panel having a distinct specificity for antigens located in the ciliary zone of either the olfactory or respiratory epithelium of rats. Tissue specificity was confirmed in enzyme-linked immunosorbent assays on membrane fractions from various tissues. During ontogeny, the expression of olfactory-specific antigens preceeds that of respiratory-specific antigens; this observation correlates with differences in the genesis of the respective cilia type and confirms that different molecular entities are recognized. A spatial segregation of immunoreactivity in the chemosensory epithelium was observed for one of the olfactory-specific monoclonal antibodies; negative zones were located in the dorsal recess of the nasal cavity and on the tips of the turbinates. Olfactory-specific antibodies reacted with distinct polypeptide bands on Western blots from olfactory ciliary preparations.
Strotmann
J
J
Institut für Zoophysiologie, Universität Stuttgart-Hohenheim, Federal Republic of Germany.
Breer
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002454:Cell Differentiation; D002923:Cilia / Q000737:chemistry*; D004848:Epithelium / Q000737:chemistry / Q000254:growth & development; D000939:Epitopes / Q000032:analysis*; D005786:Gene Expression Regulation; D051379:Mice; D008807:Mice, Inbred BALB C; D009300:Nasal Septum; D009831:Olfactory Mucosa / Q000737:chemistry* / Q000254:growth & development; D051381:Rats; D011919:Rats, Inbred Strains; D014420:Turbinates
D000911:Antibodies, Monoclonal; D000939:Epitopes