title: Identification of a gene, MLL, that spans the breakpoint in 11q23 translocations associated with human leukemias.
abstract: Recurring chromosomal translocations involving chromosome 11, band q23, have been observed in acute lymphoid leukemias and especially in acute myeloid leukemias. We recently showed that breakpoints in four 11q23 translocations, t(4;11)(q21;q23), t(6;11)(q27;q23), t(9;11)(p22;q23), and t(11;19)(q23;p13.3), were contained within a yeast artificial chromosome clone bearing the CD3D and CD3G gene loci. We have identified within the CD3 yeast artificial chromosome a transcription unit that spans the breakpoint junctions of the 4;11, 9;11, and 11;19 translocations, and we describe two other, related transcripts that are upregulated in the RS4;11 cell line. We have named this gene MLL (myeloid/lymphoid, or mixed-lineage, leukemia.
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.
authors.forename: S
authors.initials: S
authors.lastname: Ziemin-van der Poel
authors.forename: N R
authors.initials: NR
authors.lastname: McCabe
authors.forename: H J
authors.initials: HJ
authors.lastname: Gill
authors.forename: R
authors.initials: R
authors.lastname: Espinosa
authors.forename: Y
authors.initials: Y
authors.lastname: Patel
authors.forename: A
authors.initials: A
authors.lastname: Harden
authors.forename: P
authors.initials: P
authors.lastname: Rubinelli
authors.forename: S D
authors.initials: SD
authors.lastname: Smith
authors.forename: M M
authors.initials: MM
authors.lastname: LeBeau
authors.forename: J D
authors.initials: JD
authors.lastname: Rowley
mesh_terms: D000328:Adult; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D002675:Child, Preschool; D002871:Chromosome Banding; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005796:Genes; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014178:Translocation, Genetic
chemical_list: D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011061:Poly A; D012313:RNA; D013755:Tetradecanoylphorbol Acetate