title: Comparative effect of distal and proximal intestinal resection and bypass on the rat exocrine pancreas.
abstract: We studied the effects of small-bowel resection and bypass on pancreatic function in rats subjected to a 50% distal resection (DR), a 50% proximal resection (PR), a 50% jejunal bypass (BP) or an intestinal transection (SH) (controls). Duodenal contents were collected after cannulation (under basal conditions). Afterwards, an in vivo duodenal perfusion was made using a glucose/saline solution and perfusate was collected for 1 h. Following this, a cholecystokinin (CCK) solution was injected into the jugular vein (1 U/kg body wt.) and perfusion continued for another 1 h. Basal duodenal volume only increased in rats with a PR, and no significant changes occurred in protein content. In basal conditions, no decreases in amylase, lipase, trypsin, or chymotrypsin activities after DR, PR or BP were detected. When animals were subjected to a perfusion and CCK stimulation, no significant changes occurred in animals with BP; the volume was maintained in rats with PR and DR but a decrease in protein and enzymatic contents was found. We concluded that, in basal conditions, the lack (resections) or exclusion (BP) of 50% of the small bowel does not negatively affect the digestive function. When however, a sustained activity is required, the extirpation of intestinal surface provokes a fall in enzymatic activities and is not modified if only the intestinal transit is suppressed, as occurs in the cases of BP.
authors.affiliation: Departamento de Fisiología y Biología Animal, Facultad de Farmacia, Universidad de Sevilla, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Carreras
authors.forename: J C
authors.initials: JC
authors.lastname: Carrillo
authors.forename: M L
authors.initials: ML
authors.lastname: Murillo
authors.forename: M J
authors.initials: MJ
authors.lastname: Delgado
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D002766:Cholecystokinin / Q000494:pharmacology; D007421:Intestine, Small / Q000601:surgery*; D007581:Jejunoileal Bypass; D008049:Lipase / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D010189:Pancreatic Juice / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012636:Secretory Rate / Q000187:drug effects
chemical_list: D002766:Cholecystokinin; D008049:Lipase; D000681:Amylases