Termination of transcription in an 'in vitro' system is dependent on a polyadenylation sequence.
Using HeLa cell nuclear extract as a source of the different transcription and polyadenylation factors and reverse transcription to analyze the levels of RNA 5' and 3' to the cleavage-polyadenylation site, an in vitro assay has been established to study polyadenylation coupled to transcription directed by different adenovirus promoters. The levels of transcription 5' and 3' to the cleavage site in the L3 polyadenylation region are practically the same as described previously, however, the level of transcription 3' to the cleavage site in the SV40 early polyadenylation region decreases immediately after the cleavage site indicating a termination of the transcription.
Miralles
V J
VJ
Departament de Bioquìmica i Biologìa Molecular, Facultat de Farmàcia, Universitat de València, Spain.
D000256:Adenoviridae / Q000235:genetics; D001483:Base Sequence; D002457:Cell Extracts; D002872:Chromosome Deletion; D006367:HeLa Cells; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011061:Poly A / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic
D002457:Cell Extracts; D012333:RNA, Messenger; D011061:Poly A; D012319:RNA Polymerase II; D012194:RNA-Directed DNA Polymerase