title: Genetic differences accounting for evolution and pathogenicity of simian immunodeficiency virus from a sooty mangabey monkey after cross-species transmission to a pig-tailed macaque.
abstract: We determined the nucleotide sequences of two related isolates of simian immunodeficiency virus from the sooty mangabey monkey (SIVsmm) that exhibit dramatic differences in virulence. These isolates are separated by one experimental cross-species transmission, from sooty mangabey to pig-tailed macaque. The parental virus (SIVsmm9), nonpathogenic in the original host (sooty mangabeys), causes a chronic AIDS-like disease in macaques. In contrast, the variant virus (SIVsmmPBj14) induces an acute lethal disease in various macaque species and is also pathogenic for sooty mangabeys. The combination of necessary and sufficient mutations that determined the acutely lethal phenotype on the SIVsmm9 genetic background is included within a maximal set of 57 point mutations, plus two insertions located in the long terminal repeat (22 bp spanning an NF-kappa B-like enhancer element) and in the surface envelope glycoprotein (5 amino acids). Comparisons of synonymous and nonsynonymous nucleotide substitutions in the genome of SIVsmm indicated that selective pressures, probably due to the host immune response, favored amino acid changes in the envelope. This immunoevolutionary mechanism could explain the increase in diversity and the apparition of new virulent phenotypes after cross-species transmission.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U75 CHU Necker, Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Courgnaud
authors.forename: F
authors.initials: F
authors.lastname: Lauré
authors.forename: P N
authors.initials: PN
authors.lastname: Fultz
authors.forename: L
authors.initials: L
authors.lastname: Montagnier
authors.forename: C
authors.initials: C
authors.lastname: Bréchot
authors.forename: P
authors.initials: P
authors.lastname: Sonigo
mesh_terms: D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002478:Cells, Cultured; D016665:Cercocebus atys; D004279:DNA, Viral; D015752:Genes, env; D006801:Humans; D008254:Macaca nemestrina; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D016097:Simian Acquired Immunodeficiency Syndrome / Q000382:microbiology / Q000635:transmission*; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000472:pathogenicity; D013045:Species Specificity
chemical_list: D004279:DNA, Viral