Inactivation of human immunodeficiency virus type 1 by ozone in vitro.
A device was designed to deliver a constant source of given concentrations of ozone to fluids containing human immunodeficiency virus type 1 (HIV-1). Ozone was found to inactivate HIV-1 virions in a dose-dependent manner. Greater than 11 log inactivation was achieved within 2 hours at a concentration of 1,200 ppm ozone. Similar concentrations of ozone had minimal effect on factor VIII activity in both plasma and immunoaffinity-purified preparations of factor VIII treated for the same time period. The data indicate that the antiviral effects of ozone include viral particle disruption, reverse transcriptase inactivation, and/or a perturbation of the ability of the virus to bind to its receptor on target cells. Ozone treatment offers promise as a means to inactivate human retroviruses in human body fluids and blood product preparations.
Wells
K H
KH
Department of Medicine, SUNY Health Science Center, Syracuse 13210.
Latino
J
J
Gavalchin
J
J
Poiesz
B J
BJ
D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001826:Body Fluids / Q000382:microbiology; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005169:Factor VIII / Q000378:metabolism; D016655:HIV Core Protein p24 / Q000032:analysis / Q000378:metabolism; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D010126:Ozone / Q000008:administration & dosage / Q000494:pharmacology*; D018894:Reverse Transcriptase Inhibitors
D000998:Antiviral Agents; D016655:HIV Core Protein p24; D018894:Reverse Transcriptase Inhibitors; D010126:Ozone; D005169:Factor VIII; D054303:HIV Reverse Transcriptase