Lack of 5-methylcytosine in Dictyostelium discoideum DNA.
We find no evidence for the presence of 5-methylcytosine in the DNA of Dictyostelium discoideum. Methylation was absent from CCGG sites in repetitive DNA and in DNA from the actin multigene family. Nor was 5-methylcytosine detected in total DNA when base composition was determined by means of h.p.l.c.
Smith
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SS
Department of Cell and Tumor Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010.
Ratner
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DI
D044503:5-Methylcytosine; D000199:Actins / Q000235:genetics; D001483:Base Sequence; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D004023:Dictyostelium / Q000235:genetics*; D005800:Genes, Fungal; D015183:Restriction Mapping
D000199:Actins; D004271:DNA, Fungal; D044503:5-Methylcytosine; D003596:Cytosine; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific