High performance liquid chromatography (HPLC): a simple method to quantify Hb C, O-Arab, Agenogi and F.
Differentiation of some abnormal haemoglobins, such as Hb C, O-Arab, Agenogi, E, O-Indonesia, C-Harlem, and Siriraj, is difficult and quantitation of the various fractions is impossible with cellulose acetate electrophoresis. The authors report 13 cases of Hb C, 10 of Hb O-Arab and 5 of Hb Agenogi whose haemoglobin fractions were quantitated by HPLC during a thalassaemia screening programme. Hb F was determined by both Betke's method and HPLC. Analysis of data by linear regression demonstrates that the methods furnish overlapping results. Our findings show that HPLC is a rapid and easily reproduced method which allows quantitative and qualitative discrimination of the various haemoglobin fractions, making it a valid tool in screening programmes for haemoglobinopathies.
Samperi
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P
Cattedra di Ematologia Pediatrica, Università di Catania, Italy.
Mancuso
G R
GR
Dibenedetto
S P
SP
Di Cataldo
A
A
Ragusa
R
R
Schilirò
G
G
D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005319:Fetal Hemoglobin / Q000032:analysis*; D006444:Hemoglobin C / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D008403:Mass Screening; D008875:Middle Aged; D015203:Reproducibility of Results; D013789:Thalassemia / Q000175:diagnosis*
D006455:Hemoglobins, Abnormal; D006444:Hemoglobin C; C057245:hemoglobin Agenogi; D005319:Fetal Hemoglobin; C032031:hemoglobin Arab