Assignment of the gene for human tenascin to the region q32-q34 of chromosome 9.
Tenascin (TN) is a hexameric extracellular matrix glycoprotein that is highly expressed in solid tumors but has a restricted distribution in normal adult tissues. Each TN subunit is composed of segments with high homology to the sequences of epidermal growth factor, fibronectin and fibrinogen. Furthermore, it has been suggested that TN could modulate epithelial-mesenchymal and neuronal-glial interactions. Here, using a cDNA probe to human TN, we have carried out Southern blot analysis of the genomic DNAs from a panel of human-hamster somatic cell hybrids carrying different complements of human chromosomes. The results demonstrate that the human TN gene is located on chromosome 9. Furthermore, in situ hybridization studies demonstrate that human TN is located at 9q32-q34.
Rocchi
M
M
Laboratory of Molecular Genetics, Istituto G. Gaslini, Genoa, Italy.
Archidiacono
N
N
Romeo
G
G
Saginati
M
M
Zardi
L
L
D000818:Animals; D015139:Blotting, Southern; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D006224:Cricetinae; D015342:DNA Probes; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D009363:Neoplasm Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D019063:Tenascin
D015816:Cell Adhesion Molecules, Neuronal; D015342:DNA Probes; D016326:Extracellular Matrix Proteins; D009363:Neoplasm Proteins; D019063:Tenascin