title: Nucleo-cytoplasmic incompatibility in cybrid plants possessing an Atropa genome and a Nicotiana plastome.
abstract: Twenty-nine cybrids possessing an Atropa belladonna nuclear genome and a Nicotiana tabacum plastome were selected from two independent protoplast fusion experiments. In contrast to the previously described reciprocal, green and fertile cybrids with a Nicotiana nuclear genome and an Atropa plastome (Kushnir et al. 1987), the plants obtained were totally chlorophyll-deficient. An Atropa nuclear genome and a Nicotiana plastome from these chlorophyll-deficient cybrids were combined with an Atropa or a Scopolia plastome and a Nicotiana nuclear genome, respectively, in control fusion experiments. All of these nuclear genome/plastome combinations gave rise to normal, green plants. Therefore, we conclude that an N. tabacum plastome is incompatible with an A. belladonna nuclear genome.
authors.affiliation: Institute of Cell Biology and Genetic Engineering, Ukrainian Academy of Sciences, Kiev, USSR.
authors.forename: S
authors.initials: S
authors.lastname: Kushnir
authors.forename: E
authors.initials: E
authors.lastname: Babiychuk
authors.forename: M
authors.initials: M
authors.lastname: Bannikova
authors.forename: V
authors.initials: V
authors.lastname: Momot
authors.forename: I
authors.initials: I
authors.lastname: Komarnitsky
authors.forename: N
authors.initials: N
authors.lastname: Cherep
authors.forename: Y
authors.initials: Y
authors.lastname: Gleba
mesh_terms: D000681:Amylases / Q000378:metabolism; D001532:Atropa belladonna / Q000032:analysis / Q000235:genetics*; D002459:Cell Fusion; D002467:Cell Nucleus / Q000378:metabolism*; D002734:Chlorophyll / Q000032:analysis; D003593:Cytoplasm / Q000378:metabolism*; D017343:Genes, Plant; D006822:Hybrid Cells; D007527:Isoenzymes / Q000378:metabolism; D010641:Phenotype; D010946:Plants, Medicinal; D010947:Plants, Toxic; D014026:Nicotiana / Q000032:analysis / Q000235:genetics*
chemical_list: D007527:Isoenzymes; D002734:Chlorophyll; D000681:Amylases