Liposomes as a model for the study of the mechanism of fish toxicity of sodium dodecyl sulfate in sea water.
The mechanism underlying the shark repellency of SDS was studied by comparing it with the shark nonrepelling detergent, Triton X-100. The findings can be summarized as follows: (1) The effective concentration of SDS for termination of shark tonic immobility (an immediate and fast response) was close to its critical micellar concentration in sea water (70 microM). The fish lethal concentrations (LD50) were far below the CMC value for SDS, and at CMC level for Triton X-100. (2) In sea water SDS possesses a strong affinity for lipid membranes, expressed in a lipid sea water partition coefficient (Kp) of about 3000. (3) In liposomal systems examined by assays of turbidity, fluorescence resonance energy transfer and kinetics of carboxyfluorescein (CF) release, the pattern of SDS induced changes in the phospholipid bilayer suggests: (a) absence of vesicle-vesicle fusion; (b) occurrence of vesicle size increase, and (c) nonlytic gradual release of CF above and below its CMC values. In contrast, Triton X-100 above its CMC induces membrane solubilization. (4) Assays coupling CF release from liposomes to potassium diffusion potential induced by valinomycin indicate that SDS related CF release can also be attributed to a specific mechanism such as cation pore formation and not only to membrane solubilization. The hypothesis of pore formation by SDS is discussed.
Kalmanzon
E
E
H. Steinitz Marine Biological Laboratory, Interuniversity Institute of Eilat, Israel.
Zlotkin
E
E
Cohen
R
R
Barenholz
Y
Y
D000818:Animals; D003902:Detergents; D005452:Fluoresceins / Q000737:chemistry; D007700:Kinetics; D007928:Lethal Dose 50; D008081:Liposomes; D017830:Octoxynol; D011092:Polyethylene Glycols; D012623:Seawater; D012754:Sharks; D012967:Sodium Dodecyl Sulfate / Q000633:toxicity*; D013696:Temperature
D003902:Detergents; D005452:Fluoresceins; D008081:Liposomes; C024098:6-carboxyfluorescein; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D017830:Octoxynol