[A method of determining heparin in blood plasma based on its antithrombin activity].
The suggested method for measuring blood plasma heparin is based on heparin ability to enhance antithrombin activity of antithrombin III (AT-III), the major Xa and thrombin inhibitor. The method consists in measurement of blood plasma AT-III activity in the presence and absence of protamine sulfate that destroys the heparin--AT-III complex. Heparin content in U/ml is determined from the difference in the activities of heparin--AT-III complex and AT-III proper activity represented on the calibration curve. The method is sufficiently sensitive, it permits registration of heparin concentrations in a wide band (from 0.01 U/ml to 0.75 U/ml of plasma).
Taktashev
R E
RE
Suvorova
A V
AV
Miller
V E
VE
Kolesnikova
O I
OI
D000990:Antithrombin III / Q000378:metabolism*; D006493:Heparin / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D011479:Protamines
D007202:Indicators and Reagents; D011479:Protamines; D000990:Antithrombin III; D006493:Heparin
Metod opredeleniia geparina v plazme krovi po ego antitrombinovoĭ aktivnosti.