Immunohistochemical localization of the matrix glycoproteins--tenascin and the ED-sequence-containing form of cellular fibronectin--in human permanent teeth and periodontal ligament.
The expression of two matrix glycoproteins, tenascin and cellular fibronectin (cFN), has been studied in fully developed human permanent teeth, periodontal ligament, and alveolar bone, in both frozen and paraffin-processed material. Polyclonal antibodies to tenascin and a monoclonal antibody recognizing the ED sequence specific to at least some forms of cFN were used. Staining for both tenascin and cFN was positive in the dental pulp, odontoblastic layer, cementoblast-pre-cementum zone, and on the periosteal as well as endosteal surfaces of the alveolar bone. In the periodontal ligament, cFN was evenly distributed, whereas tenascin was accumulated in the attachment zones. Pre-dentin stained for tenascin but not for cFN. Mineralized dentin and cementum were tenascin- and cFN-negative. The relative staining intensity for tenascin was greater than that for cFN in the cementoblast-pre-cementum layer and in the attachment zones of the periodontal ligament, whereas cFN stained more intensely in the pulp. In frozen material, antigenicities were well-preserved. Paraffin processing facilitated precise recognition of tissue morphology, but the antigenicity of cFN was lost. The co-expression of tenascin and cFN in the dental pulp, cementogenic zone, and on the surfaces of the alveolar bone may reflect the ability of the cells to deposit mineralized tissue matrices. The pronounced expression of tenascin in the interfaces between mineralized and non-mineralized tissues suggests that it is functionally associated with mechanical stress and may thus have at least two distinct functions. The relative amounts of the two matrix glycoproteins may contribute to regulation of tissue structure.
Lukinmaa
P L
PL
Department of Dental Radiology/Oral Pathology, University of Helsinki, Finland.
Mackie
E J
EJ
Thesleff
I
I
D000328:Adult; D000539:Alveolar Process / Q000737:chemistry / Q000648:ultrastructure; D000911:Antibodies, Monoclonal; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry*; D003739:Dental Cementum / Q000737:chemistry / Q000648:ultrastructure; D003782:Dental Pulp / Q000737:chemistry / Q000648:ultrastructure; D003804:Dentin / Q000737:chemistry / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D003012:Microbial Collagenase / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D010513:Periodontal Ligament / Q000737:chemistry* / Q000648:ultrastructure; D010521:Periosteum / Q000737:chemistry / Q000648:ultrastructure; D019063:Tenascin; D014070:Tooth / Q000737:chemistry* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D009419:Nerve Tissue Proteins; D019063:Tenascin; D003012:Microbial Collagenase