Corticotropin-releasing factor (CRF) gene family in the brain of the teleost fish Catostomus commersoni (white sucker): molecular analysis predicts distinct precursors for two CRFs and one urotensin I peptide.
Molecular cloning experiments indicate the presence of two distinct CRF genes in the sucker genome encoding independent 162-amino-acid precursors, which both consist of a signal sequence, succeeded by a cryptic peptide and subsequently by the hormone moiety. The two 41-amino-acid CRF peptides differ by an Ala-->Val substitution at amino acid position 28. CRF transcripts are primarily found in the sucker pre-optic nucleus (PON), to a much lesser extent in the lateral tuberal nucleus (LTN). In contrast, urotensin I (U I) encoding mRNA is equally present in both tissues. In urophysectomized fish, U I mRNA is elevated especially in LTN tissue, while CRF mRNA levels remain more or less constant in the PON and LTN regions.
Morley
S D
SD
Institut für Zellbiochemie und klinische Neurobiologie, Universität Hamburg, Federal Republic of Germany.
Schönrock
C
C
Richter
D
D
Okawara
Y
Y
Lederis
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D003001:Cloning, Molecular; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D005399:Fishes / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D005810:Multigene Family; D011061:Poly A / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D011301:Preoptic Area / Q000737:chemistry / Q000201:enzymology; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics; D012313:RNA / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014579:Urotensins / Q000737:chemistry / Q000235:genetics*
D011498:Protein Precursors; D012333:RNA, Messenger; D014579:Urotensins; D011061:Poly A; D012313:RNA; D003346:Corticotropin-Releasing Hormone; C029676:urotensin I