Immunoregulatory effects of transforming growth factor-beta in a prolonged period of culture.
It is well known that transforming growth factor-beta (TGF-beta) strikingly inhibits numerous immune functions in short-term cultures. In this study we investigated the effects of TGF-beta on the immune responses of murine spleen cells in a prolonged period of culture. The addition of exogenous TGF-beta (0.1 ng/ml) inhibited the proliferation of Con A- or LPS-stimulated spleen cells, polyclonal IgM and IgG antibody production, and NK cell activity during 4 days of the initial culture and subsequently enhanced their responses on Day 10. The augmented polyclonal IgM and IgG responses in murine spleen cells induced by LPS and TGF-beta on Day 10 were suppressed by the secondary addition of TGF-beta on Day 6. These results suggest that TGF-beta acts as an immunoregulator in prolonged period responses by immunoactivators.
Ishizaka
S
S
Third Department of Internal Medicine, Nara Medical University, Japan.
Yoshikawa
M
M
Tsujii
T
T
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000008:administration & dosage; D007111:Immunity, Cellular / Q000187:drug effects; D007113:Immunity, Innate / Q000187:drug effects; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D008070:Lipopolysaccharides; D016212:Transforming Growth Factor beta; D003208:Concanavalin A