Identification of a sterility-inducing cytoplasm in a fertile accession line of Phaseolus vulgaris L.
Previous investigations into the genetic mechanism of fertility restoration in cytoplasmic male sterile Phaseolus vulgaris suggested that this is a particularly interesting system for the study of nuclear-mitochondrial interactions. This study was conducted to investigate the nature of nuclear-mitochondrial compatibility in fertile accession line G08063, the reported progenitor to the cytoplasmic male sterile line. Results from genetic analysis indicated that fertile line G08063 carried a sterility-inducing cytoplasm with a fertility restoring nuclear genotype. Mitochondrial DNA analysis indicated that the mechanism of fertility restoration by line G08063 was different from that conditioned by Fr, a previously described restorer gene. A mitochondrial DNA sequence associated with sterility and lost upon fertility restoration by nuclear gene Fr was present in the mitochondrial genome of fertile line G08063; this sequence was not carried within the mitochondrial genome of any other P. vulgaris accession line tested.
Mackenzie
S A
SA
Department of Agronomy, Purdue University, West Lafayette, Indiana 47907.
D002467:Cell Nucleus / Q000502:physiology; D003433:Crosses, Genetic; D003593:Cytoplasm / Q000502:physiology; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D007887:Fabaceae / Q000235:genetics / Q000502:physiology*; D005298:Fertility; D005838:Genotype; D008928:Mitochondria / Q000502:physiology; D010946:Plants, Medicinal; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D000077278:RNA, Mitochondrial
D004272:DNA, Mitochondrial; D000077278:RNA, Mitochondrial; D012313:RNA