Cloning of a low-temperature-induced gene lti2 from the cyanobacterium Anabaena variabilis M3 that is homologous to alpha-amylases.
A gene called lti2 which is induced by a shift to a low temperature was isolated from the cyanobacterium Anabaena variabilis strain M3. This gene contained an open reading frame of 552 amino acid residues which exhibited homology to known alpha-amylases. The level of lti2 transcript increased 40-fold within an hour after a temperature shift from 38 to 22 degrees C and then slowly decreased to a low steady-state level. A less extensive expression of the lti2 gene is also induced by a temperature shift from 22 to 38 degrees C.
Sato
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Department of Botany, Faculty of Science, University of Tokyo, Japan.
D000595:Amino Acid Sequence; D017033:Anabaena / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D015870:Gene Expression / Q000502:physiology; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D000516:alpha-Amylases / Q000737:chemistry / Q000235:genetics*
D001426:Bacterial Proteins; D000516:alpha-Amylases