Copper stained protein gels can be efficiently used for immunoblotting.
We have found that the previously described fast and sensitive copper staining of proteins resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis does not interfere with the subsequent electrotransfer of these proteins to a solid support and their detection by specific antibodies. After the gel is copper stained and photographed it is simply destained and then equilibrated in transfer buffer prior to immunoblotting. We find that this treatment has no significant effect on transfer efficiency or band sharpness and is compatible with all common detection methods for the blotted proteins. It thus permits the separation of proteins to be checked in a simple way before immunoblotting is performed.
Seeber
F
F
Institut für Tropenhygiene und Offentliches Gesundheitswesen, University of Heidelberg, Germany.
Lucius
R
R
D003300:Copper; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling
D011506:Proteins; D003300:Copper