title: A rapid method for purification of synthetic oligoribonucleotides.
abstract: A procedure for large-scale purification of synthetic oligoribonucleotides has been developed that has significant advantages over gel purification techniques currently in use. Synthesis was performed using commercially available 2'-O-silylated ribonucleoside 3'-O-phosphoramidites, and coupling efficiencies were consistently greater than 97% for oligoribonucleotides up to 31 residues in length. Using C4 reverse-phase chromatography to remove material not deprotected by treatment with tetrabutylammonium fluoride, we have eliminated reactants in which the 2'-O-silyl group is only partly removed, thus ensuring a homogeneous population of oligoribonucleotide.
authors.affiliation: Yale University School of Medicine, New Haven, CT 06510-0824.
authors.forename: K R
authors.initials: KR
authors.lastname: Webster
authors.forename: Y
authors.initials: Y
authors.lastname: Shamoo
authors.forename: W
authors.initials: W
authors.lastname: Konigsberg
authors.forename: E K
authors.initials: EK
authors.lastname: Spicer
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D003902:Detergents; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification*; D000644:Quaternary Ammonium Compounds; D012313:RNA / Q000302:isolation & purification; D014764:Viral Proteins
chemical_list: D003902:Detergents; D009843:Oligoribonucleotides; D000644:Quaternary Ammonium Compounds; C066700:RegA protein, Enterobacteria phage T4; D014764:Viral Proteins; D012313:RNA; C009405:tetrabutylammonium