Mode of action of 2-(diethylamino)-7-ethoxychromone on human platelets.
The in vitro effect of 2-(diethylamino)-7-ethoxychromone (RC39XVIII) on human platelet aggregation induced by several agonists and on thromboxane B2 formation, granule release and intracellular cAMP elevation has been studied. The chromosome-derivative exerts a dose-dependent inhibitory effect on aggregation produced by U46619, arachidonic acid, thrombin, collagen and ADP. RC39XVIII inhibits aggregation, TxB2 formation and granule release in parallel. Moreover the drug potentiates cAMP accumulation induced by iloprost and forskolin. The drug also inhibits soluble cAMP phosphodiesterase in a dose-dependent manner. No effect on adenylate cyclase activity measured in platelet membranes was evident.
Leoncini
G
G
Istituto di Chimica Biologica, Università di Genova, Italy.
Maresca
M
M
Colao
C
C
Buzzi
E
E
Mazzei
M
M
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism; D002867:Chromones / Q000494:pharmacology*; D005576:Colforsin / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D016285:Iloprost / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology; D013928:Thromboxane A2 / Q000378:metabolism
D002867:Chromones; D010975:Platelet Aggregation Inhibitors; D011450:Prostaglandin Endoperoxides, Synthetic; C071473:2-(diethylamino)-7-ethoxychromone; D005576:Colforsin; D013928:Thromboxane A2; D000244:Adenosine Diphosphate; D003094:Collagen; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D013917:Thrombin; D000262:Adenylyl Cyclases; D016285:Iloprost