[The low-molecular protein of the cell wall in streptococci group A devoid of serological type specificity].
The scheme for the isolation and purification of low-molecular cell-wall protein without type specificity, including the extraction of the cell walls of group A streptococci, type M 29, with 1% solution of Triton X-100, the separation of the extract by ion-exchange chromatography in DEAE-trisacryl M with the subsequent two-stage gel filtration in superfine Sephadex G-50, is described. The isolated protein had a molecular weight of 4,000 daltons and contained no admixtures of group-specific polysaccharide A, phosphorus, nucleic acids and Fc receptors and interacted with antisera to group A streptococcal cells of heterologous type M in the enzyme immunoassay (EIA). Purified protein was characterized by a high content of glycine. The antigenic determinants of immobilized protein, recognized by antibodies in EIA, were sensitive to the action of trypsin and resistant to the action of pepsin, papain, pronase E and sodium periodate.
Molchanova
T O
TO
Shikhman
A R
AR
Dynga
L O
LO
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002458:Cell Fractionation / Q000379:methods; D002473:Cell Wall / Q000737:chemistry / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D007114:Immunization; D008970:Molecular Weight; D011817:Rabbits; D012703:Serotyping; D013297:Streptococcus pyogenes / Q000032:analysis* / Q000145:classification / Q000276:immunology
D000596:Amino Acids; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes
Nizkomolekuliarnyĭ belok kletochnoĭ stenki streptokokkov gruppy A, lishennyĭ tipovoĭ serologicheskoĭ spetsifichnosti.