De novo chromosome formation in rodent cells.
A hybrid cell line was produced by the fusion of an EC3/7 mouse cell with a Chinese hamster ovary cell. The EC3/7 cell carries a dicentric chromosome with a functional marker centromere. This marker centromere contains human, lambda, and bacterial vector DNA sequences and a dominant selectable gene (aminoglycoside 3'-phosphotransferase type II; neo). In the hybrid, the marker centromere separated from the dicentric chromosome and formed a full-sized chromosome (lambda neo). The newly formed chromosome is stable, even under nonselective culture conditions. This functional chromosome, which is the result of an amplification process, is composed of seven large, different-sized amplicons. Each amplicon contains multiple copies of human, lambda, neo, and mouse telomeric DNA sequences. Individual amplicons are separated from each other by mouse major satellite DNA sequences. The marker centromere was localized to a terminal amplicon by anticentromere immunostaining. The number of amplicons in the newly formed chromosome is remarkably consistent. This finding suggests that the length of the newly formed chromosome is highly constrained.
Praznovszky
T
T
Institute of Genetics, Hungarian Academy of Sciences, Szeged.
Keresö
J
J
Tubak
V
V
Cserpán
I
I
Fátyol
K
K
Hadlaczky
G
G
D000818:Animals; D006690:Bisbenzimidazole; D016466:CHO Cells; D002459:Cell Fusion; D002460:Cell Line; D002503:Centromere / Q000648:ultrastructure; D002875:Chromosomes / Q000502:physiology / Q000648:ultrastructure*; D006224:Cricetinae; D015342:DNA Probes; D005455:Fluorescent Antibody Technique; D006822:Hybrid Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D016615:Telomere / Q000648:ultrastructure
D015342:DNA Probes; D006690:Bisbenzimidazole