title: Report of studies on monoclonal anti-IgG antibodies.
abstract: Eleven monoclonal anti-IgG antibodies were evaluated by ten laboratories for possible AHG reagent use by the ISBT/ICSH protocol. Three of the anti-IgGs were selected by tests with weak Fya sensitized cells as this represents the best screening test. One of the IgM class anti-IgGs (205) equalled ISBT/ICSH reference reagents (R3P and RIIIM) against weak anti-D, -K and Fya sensitized cells and it reacted with all four subclasses of IgG. Thus, subject to satisfactory stability and extended trials with a wide range of weak antibodies, monoclonal anti-IgGs for AHG reagent use are now a possible alternative to conventional rabbit anti-IgG.
authors.affiliation: Regional Transfusion and Immuno-Haematology Centre, Cambridge, U.K.
authors.forename: D
authors.initials: D
authors.lastname: Voak
authors.forename: U
authors.initials: U
authors.lastname: Nilsson
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D000915:Antibody Affinity; D000939:Epitopes / Q000032:analysis; D004912:Erythrocytes / Q000276:immunology; D005189:False Positive Reactions; D006801:Humans; D007074:Immunoglobulin G
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G