Electrical parameters of the toad skin: effects of forskolin.
Forskolin stimulated short-circuit current (SCC) and transepitelial electrical conductance (G) in the isolated skin of the toad Bufo arenarum in a concentration-dependent manner, between 1.0 x 10(-6) and 2.4 x 10(-5) M. At the latter concentration, glandular secretion appeared to be stimulated also. The increase in G was considerably greater in skins bathed in Ringer solution than in solutions containing no chloride. The increased SCC was abolished by amiloride, a specific blocker of sodium transport in amphibian membranes, irrespective of the anion present in the solution bathing the skin. G was also decreased by amiloride to control values in skins bathed in solutions without chloride, but remained elevated in the presence of Cl-. The increase in SCC following exposure to forskolin, 4.4 x 10(-6) M, was not altered when furosemide, a specific blocker of chloride transport, was present in the Ringer solution bathing the dermal side of the skin. The response to forskolin, 2.4 x 10(-5) M, however, was significantly decreased by dermal furosemide; the inhibitor was ineffective in the absence of chloride. The data indicate that forskolin acts on at least two sites: stratum granulosum cells (the main pathway for sodium transport, and an alternate site, responsible for the increase in permeability to chloride. In addition, at high concentration of the agent, glandular secretion is also stimulated. The data suggest that the adenylate cyclase-cyclic AMP system is involved in the regulation of the permeability of the toad skin to sodium and chloride, probably by separate cell types.
Castillo
G A
GA
Dept. of Physiology, INSIBIO (UNT-CONICET), Tucumán, Argentina.
Coviello
A
A
Orce
G G
GG
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002022:Bufo arenarum / Q000502:physiology*; D002712:Chlorides / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology*; D004553:Electric Conductivity; D004594:Electrophysiology; D005665:Furosemide / Q000494:pharmacology; D007700:Kinetics; D012867:Skin / Q000187:drug effects; D012879:Skin Physiological Phenomena
D002712:Chlorides; D005576:Colforsin; D000584:Amiloride; D005665:Furosemide