Definition of platelet-derived histamine-releasing factor and histaminergic receptors modulating platelet aggregation.
Endogenous and exogenous free radical scavengers significantly decrease mast cell histamine release induced by coincubation with resting, activated platelets or with platelet-derived supernatant. Histamine and pyridylethylamine dose-dependently enhance platelet aggregation; the effect is potentiated by ranitidine and blocked by mepyramine. Histamine increases also cytosolic calcium concentration in platelets stimulated with thrombin, and binding sites for [3H]-mepyramine are present on platelet membranes.
Mannaioni
P F
PF
Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.
Pistelli
A
A
Gambassi
F
F
Di Bello
M G
MG
Raspanti
S
S
Masini
E
E
D001792:Blood Platelets / Q000737:chemistry / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D005609:Free Radicals; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D010974:Platelet Aggregation; D011968:Receptors, Histamine / Q000502:physiology*
D005609:Free Radicals; D011968:Receptors, Histamine; D002118:Calcium