Manganese ion elicits a binding activity of placenta vitronectin receptor to fibronectin cell-binding domain.
Some members of the integrin family recognize the RGD sequence which is common to cell adhesive proteins in a divalent cation-dependent manner. In the presence of Ca2+ and Mg2+, the fibronectin receptor of placenta recognizes the RGD sequence of fibronectin, but not that of vitronectin, while the vitronectin receptor of placenta recognizes the RGD sequence of vitronectin, but not that of fibronectin, although both receptors recognize the same RGD sequence. We have found by performing an enzyme-linked immunosorbent assay (ELISA) using receptor-specific monoclonal antibodies and by electrophoretic analysis that in the presence of Mn2+ a vitronectin receptor of placenta binds to an affinity column coupled with the cell-binding domain of fibronectin. By replacing divalent cations from Mn2+ to Ca2+ and Mg2+, the vitronectin receptor was completely eluted from the column. When the synthetic peptides GRGDSP and GRGESP were applied to the column as competitors, the Mn(2+)-dependent binding was inhibited by both peptides. These results suggest that Mn2+ elicits a binding activity of the placenta vitronectin receptor to the RGD site of fibronectin. The modulation of ligand specificity by Mn2+ will provide an important clue in the elucidation of the cause of individual ligand specificity of RGD-recognizing integrins.
Yanai
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T
Tissue Culture Laboratory, Iwaki Glass Company, Chiba, Japan.
Shimo-Oka
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Ii
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D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005353:Fibronectins / Q000276:immunology / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D016023:Integrins / Q000235:genetics / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology*; D009842:Oligopeptides / Q000032:analysis / Q000378:metabolism; D010920:Placenta / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D011247:Pregnancy; D011971:Receptors, Immunologic / Q000187:drug effects / Q000378:metabolism*; D019030:Receptors, Vitronectin; D019096:Vitronectin
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D006023:Glycoproteins; D016023:Integrins; D009842:Oligopeptides; D011971:Receptors, Immunologic; D019030:Receptors, Vitronectin; D019096:Vitronectin; D008345:Manganese; C047981:arginyl-glycyl-aspartic acid