Alpha-fetoprotein: a sensitive avidine-biotin assay.
A conventional enzyme-linked immunosorbent assay (ELISA) for alpha-fetoprotein was modified to incorporate the avidine-biotin complex, in order to enhance sensitivity. Five modifications of this assay were compared, of which the 2-step noncompetitive avidine-biotin assay (NABA) was found to be the most rapid and sensitive (detection limit 0.05 ng/ml). Levels of AFP in human cord serum measured by the 2-step NABA method showed good correlation (r = 0.965) with measurements by radio-immuno assay, and the assay can be performed within one hour. The simplicity and sensitivity of this assay should make it a reliable method of choice for determination of alpha-fetoprotein.
Chakrabarty
M
M
Indian Institute of Chemical Biology, Calcutta, India.
Bhattacharyya
M
M
Mandal
C
C
D001360:Avidin; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005312:Fetal Blood / Q000737:chemistry; D006801:Humans; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins; D001360:Avidin; D001710:Biotin