Immunological memory for HIV-1 induced in rabbits by immune poly(A)+ RNA.
New Zealand rabbits were used to demonstrate the in vitro and in vivo transfer of reactivity, including immunological memory, to a synthetic peptide corresponding to residues 586-606 of the gp-160 protein of human immunodeficiency virus (HIV-1). The transfer were mediated by immune poly (A)+ RNA from lymphoid organs (spleen and mesenteric nodules) harvested after immunization of a sheep with the peptide (8 subcutaneous injections plus glucan and complete Freund's adjuvant using a total of 1750 micrograms peptide). Immunological reactivity was detected by the leukocyte adherence inhibition (LAI) test for cellular immunity. A dose of 150 micrograms poly(A)- RNA ml-1 10(7) leukocytes-1 or 2.0 micrograms poly(A)+ RNA ml-1 10(7) leukocytes-1 was used for in vitro transfer. For in vivo transfer the recipient rabbits received 3,000 micrograms poly(A)- RNA or 20 micrograms poly(A)+ RNA. The mean non-adherence index (NAI) obtained in vitro was 10 +/- 7 for leukocytes treated with poly(A)-RNA and 60 +/- 10 leukocytes treated with poly(A)+ RNA. The poly(A)+ RNA fraction induced a primary-like response and memory cells in vivo. The poly(A)-RNA fraction had no effect. Since sheep are refractory to, and rabbits are sensitive to HIV-1, we suggest the use of this animal model for testing the immunomodulating effect of anti-HIV-1 immune poly(A)+ RNA.
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Departamento de Morfologia, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Brasil.
D000818:Animals; D005260:Female; D015488:HIV Antigens / Q000276:immunology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D007117:Immunization, Secondary; D007156:Immunologic Memory; D007957:Leukocyte Adherence Inhibition Test; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D011061:Poly A / Q000276:immunology*; D012313:RNA / Q000276:immunology*; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination
D015488:HIV Antigens; D008562:Membrane Glycoproteins; D011061:Poly A; D012313:RNA