Growth of Chinese hamster ovary (CHO) cells in the presence of MMC in low-serum medium.
Chinese hamster ovary (CHO-WBLT) cells growing in McCoy's 5a with 10% fetal bovine serum (FBS) were adapted to 0.5% FBS in CHO-1 Complete Media System, a serum-free medium from Ventrex. Cells in these two media were exposed to 10(-7) M and 10(-8) M mitomycin C (MMC) for 24 h. Comparison of cell growth over 10 days showed that cells in 0.5% serum proliferate, though at a slower rate than cells in 10% serum. Treatment with MMC revealed that at 10(-7) M, MMC is cytotoxic to cells to both the media; at 10(-8) M, MMC is non-cytotoxic to cells in both media.
Sharma
K
K
College of Arts and Sciences, Western Michigan University, Kalamazoo 49008.
Milanowski
D
D
Ficsor
G
G
D000818:Animals; D016466:CHO Cells; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D003470:Culture Media; D007700:Kinetics; D016685:Mitomycin / Q000494:pharmacology*
D003470:Culture Media; D016685:Mitomycin