Crystallization and preliminary characterization of mitogillin, a ribosomal ribonuclease from Aspergillus restrictus.
Mitogillin is a ribonuclease secreted by the fungus Aspergillus restrictus. The substrate for mitogillin is a short, universally conserved, sequence in ribosomal RNA. Cleavage of this sequence inactivates protein synthesis. Mitogillin was crystallized by a two-chamber vapor/liquid diffusion method using ethanol as the precipitant. This method has wider potential in the use of volatile organic solvents as precipitants. Crystals of mitogillin diffract X-rays to lattice d-spacings of at least 1.6 A, and belong to the monoclinic space group P2(1), with a = 50.4 A, b = 82.4 A, c = 38.2 A and beta = 99.8 degrees.
Martinez
S E
SE
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.
Smith
J L
JL
D000485:Allergens; D000818:Animals; D052179:Antigens, Plant; D001230:Aspergillus / Q000201:enzymology*; D001483:Base Sequence; D003460:Crystallization; D005656:Fungal Proteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D012313:RNA / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases / Q000737:chemistry*; D012270:Ribosomes / Q000201:enzymology*; D014961:X-Ray Diffraction
C081947:ASPF1 protein, Aspergillus fumigatus; D000485:Allergens; D052179:Antigens, Plant; D005656:Fungal Proteins; D010455:Peptides; C036196:MITF protein, Aspergillus restrictus; D012313:RNA; D012260:Ribonucleases