Molecular heterogeneity of angiotensin converting enzyme in human cerebrospinal fluid.
Three different molecular forms of angiotensin converting enzyme (ACE) (approximately Mr 150,000, 80,000 and 40,000, respectively), have been recovered from human cerebrospinal fluid. All three enzymes were inhibited by captopril and enalapril and their activity was potentiated by chloride ions. They were capable of degrading Leu-enkephalin-Arg6 and substance -P, but gave no conversion of neurokinin A. In all these aspects, the CSF enzymes were identical with the human pulmonary enzyme. The Mr 40,000 form of ACE is the smallest active form of the enzyme hitherto reported and is likely to represent a fragment of the C-terminal part of native ACE, where its active center is located.
Lantz
I
I
Department of Pharmacology, University of Uppsala, Sweden.
Nyberg
F
F
Terenius
L
L
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors; D002216:Captopril / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004656:Enalapril / Q000494:pharmacology; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015288:Neurokinin A / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000134:cerebrospinal fluid* / Q000737:chemistry / Q000378:metabolism; D011863:Radioimmunoassay; D013373:Substance P / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D002712:Chlorides; D013373:Substance P; D004743:Enkephalin, Leucine; D004656:Enalapril; C026768:enkephalin-Leu, Arg(6)-; D015288:Neurokinin A; D002216:Captopril; D007703:Peptidyl-Dipeptidase A