[Comparative histological and light-microscopic cytomorphometric studies of the tissue repair processes in postoperative wound complications treated locally with helium-neon laser radiation or a proteolytic enzyme].
Morphological studies were carried out on tissue material, removed from wound peripheral parts during application of secondary sutures on 69 complete perineal wound dehiscences of parturients prepared by helium-neon laser irradiation according to a method described by us, as of wound peripheral parts of 15 perineal wound dehiscences, prepared by alphahymotripsin dissolved in a solution with vitamin C. Histological preparations were stained by hematoxilin-eosin by van Gieson (for collagenous fibres), by Weigert (for elastic fibres) and by Gomori (for reticular fibres). Light microscopic cytomorphometric studies were made by a graduated ocular micrometer on stained with hematoxilin-eosin tissue sections with the help of standard optical system. The results of the performed histological examinations give foundation to accept the occurred more active stimulation of inflammatory and proliferative phase of wound reparative process after laser therapy with helium-neon laser irradiation. Light microscopic cytomorphometric studies indicate the presence of more advanced in its development granulation tissue after laser therapy by means of activation of local immunocompetent cells.
Burgudzhieva
T
T
Enchev
V
V
Bradinski
A
A
D002918:Chymotrypsin / Q000008:administration & dosage; D005260:Female; D006801:Humans; D053685:Laser Therapy; D010447:Peptide Hydrolases / Q000627:therapeutic use*; D010502:Perineum / Q000601:surgery; D013194:Staining and Labeling / Q000379:methods; D013529:Surgical Wound Dehiscence / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy; D014945:Wound Healing / Q000187:drug effects* / Q000528:radiation effects*
D010447:Peptide Hydrolases; D002918:Chymotrypsin
Sravnitelni khistologichni i svetlinnomikroskopski tsitomorfometrichni prouchvaniia na tŭkannite reparativni protsesi pri sledoperativni ranevi uslozhneniia, tretirani lokalno s kheliĭ-neonovo lazerno lŭchenie ili proteolitichen enzim.