Activation of interferon-inducible genes in vivo by synthetic double-stranded RNA, poly rI:rC.
We analyzed the effects of synthetic dsRNA (poly rI:rC) treatment on the expression in vivo of two interferon (IFN)-inducible genes. DBA/2 mice were injected i.p. with poly rI:rC and its effect on the levels of the following mRNAs was determined; 202, 2'-5' oligoadenylate synthetase (2-5A synthetase) and beta-actin. After poly rI:rC treatment the levels of the 202 and 2-5A synthetase mRNAs in the spleen and in bone marrow peaked between 12 and 24 h and decreased thereafter, whereas beta-actin levels remained unchained unchanged. Pretreatment of DBA/2 mice with sheep anti-murine IFN-alpha/beta antibodies before rI:rC injection strongly diminished the induction of 202 mRNA indicating that IFN-alpha/beta mediated this induction.
Gariglio
M
M
Institute of Microbiology, Medical School, University of Turin, Italy.
Panico
S
S
Gribaudo
G
G
Martinotti
M G
MG
Cavallo
G
G
Landolfo
S
S
D000818:Animals; D001853:Bone Marrow / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D007372:Interferons / Q000276:immunology / Q000502:physiology*; D008025:Ligases / Q000235:genetics; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D011070:Poly I-C / Q000008:administration & dosage / Q000494:pharmacology*; D012330:RNA, Double-Stranded / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000737:chemistry
D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007372:Interferons; D008025:Ligases; D011070:Poly I-C