title: [Utility of the L-alanine-aminopeptidase test for differentiating the cell wall structure of bacteria].
abstract: Evaluation of a detection test for L-alanine-aminopeptidase enzyme (Bactident Aminopeptidase) for determining the structure of bacterial cell wall. In a total of 246 clinical isolates of aerobic, microaerophilic and anaerobic bacteria, we detect the presence or absence of L-alanine-aminopeptidase using commercial kits (Bactident Aminopeptidase, Merck Diagnostica). We also identify and further classified the 246 strains. In nearly all gram-negative bacteria L-alanine-aminopeptidase was found, with the exception of Campylobacter spp and gram-negative anaerobic bacilli. All gram-positive and gram-variable bacteria were negative for the L-alanine-aminopeptidase presence. The results suggest a good correlation between the presence or absence of L-alanine-aminopeptidase and Gram stain method.
authors.affiliation: Laboratorio de Microbiología, Hospital La Inmaculada, Huercal-Overa, Almería.
authors.forename: J M
authors.initials: JM
authors.lastname: Hernández Molina
authors.forename: A
authors.initials: A
authors.lastname: Martínez
authors.forename: M C
authors.initials: MC
authors.lastname: Parra
authors.forename: M I
authors.initials: MI
authors.lastname: Ortega
mesh_terms: D000626:Aminopeptidases / Q000032:analysis*; D001424:Bacterial Infections / Q000382:microbiology; D001426:Bacterial Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques; D018826:CD13 Antigens; D002473:Cell Wall / Q000201:enzymology; D005069:Evaluation Studies as Topic; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification; D006801:Humans; D011933:Reagent Kits, Diagnostic; D013194:Staining and Labeling
chemical_list: D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; D000626:Aminopeptidases; D018826:CD13 Antigens
vernacular_title: Utilidad de la prueba de la L-alanina-aminopeptidasa para diferenciar la estructura de la pared celular de las bacterias.