Isolation of monoclonal antibodies monospecific for bovine beta-lactoglobulin.
Monoclonal antibodies were generated against purified bovine beta-lactoglobulin A. Six antibodies reactive only with beta-lactoglobulin were selected. At least one of the antibodies appeared to be species monospecific for bovine beta-lactoglobulin. The remaining five antibodies recognized proteins in caprine and porcine whey fractions. One monoclonal antibody (59-1) exhibited a distinct 2:1 preference for beta-lactoglobulin B over A at near neutral pH (7.5). These antibodies should prove extremely useful adjuncts in structural studies of bovine beta-lactoglobulin.
Kuzmanoff
K M
KM
Specialized Center for Cancer Research and Education, University of Illinois School of Medicine, Chicago 60612.
Beattie
C W
CW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006041:Goats; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D007782:Lactoglobulins / Q000276:immunology*; D011863:Radioimmunoassay; D013552:Swine
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D007782:Lactoglobulins