title: Identification of phosphotyrosine residues during protein sequence analysis.
abstract: Synthetic tyrosine-phosphorylated peptides were subjected to protein sequence analysis using a gas-phase sequencer and on-line phenylthiohydantoin (PTH) amino acid analysis. Our data show that phosphotyrosine is stable to the gas-phase sequencing chemistry and can be detected as its PTH-derivative during routine sequence analysis without the need of prior tyrosine radiolabeling.
authors.affiliation: Howard Hughes Medical Institute, University of California, San Francisco.
authors.forename: C W
authors.initials: CW
authors.lastname: Turck
authors.forename: J
authors.initials: J
authors.lastname: Herrmann
authors.forename: J A
authors.initials: JA
authors.lastname: Escobedo
authors.forename: L T
authors.initials: LT
authors.lastname: Williams
mesh_terms: D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010669:Phenylthiohydantoin; D019000:Phosphotyrosine; D011506:Proteins / Q000737:chemistry*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D011506:Proteins; D010669:Phenylthiohydantoin; D019000:Phosphotyrosine; D014443:Tyrosine