title: Extracellular ATP stimulates interleukin-dependent cultured mast cells and eosinophils through calcium mobilization.
abstract: We examined the effect of ATP and related nucleotides on the changes in intracellular calcium ([Ca2+]i) in murine bone marrow-derived mast cells (BMMC) and human cord blood-derived eosinophils (EO) cultured in the presence of interleukins. ATP, ADP and AMP released a substantial amount of histamine and leukotriene C4 from BMMC, and EO showed locomotive activity in response to ATP, ADP and GTP. These reactions were accompanied with an increase in [Ca2+]i in BMMC and in EO. The rise in [Ca2+]i in BMMC induced by ATP or antigen at optimal concentrations was inclined to be persisting. On the other hand, these nucleotides induced a rapid and transient rise in [Ca2+]i in EO. Purified human peripheral EO also exhibited locomotive activity and an increase in [Ca2+]i in response to ATP. These results indicate that extracellular ATP activates interleukin-dependent cultured mast cells and EO through Ca2+ mobilization, and suggest that ATP, which is known to be released from activated platelets or autonomic nerves, may stimulate in vivo counterparts of these cultured inflammatory cells.
authors.affiliation: Division of Allergy, National Children's Medical Research Center, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: M
authors.initials: M
authors.lastname: Ebisawa
authors.forename: D C
authors.initials: DC
authors.lastname: Reason
authors.forename: K
authors.initials: K
authors.lastname: Ohno
authors.forename: K
authors.initials: K
authors.lastname: Kurihara
authors.forename: N
authors.initials: N
authors.lastname: Sakaguchi
authors.forename: A
authors.initials: A
authors.lastname: Ohgimi
authors.forename: E
authors.initials: E
authors.lastname: Saito
authors.forename: A
authors.initials: A
authors.lastname: Akasawa
authors.forename: K
authors.initials: K
authors.lastname: Akimoto
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D007378:Interleukins / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D013189:SRS-A / Q000096:biosynthesis
chemical_list: D007378:Interleukins; D013189:SRS-A; D000255:Adenosine Triphosphate; D002118:Calcium