A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures.
A novel, simplified procedure for the removal of DNA template from newly synthesized RNA is described. The method avoids the use of DNase and involves extraction of the RNA transcription products at acid pH using either phenol or phenol: chloroform:guanidinium thiocyanate mixture. Both procedures are as effective as DNase digestion in removing DNA template, but are free of the hazard of RNA degradation, are highly reproducible and remove proteins at the same time.
Kedzierski
W
W
University of Texas, Southwestern Medical Center.
Porter
J C
JC
D002725:Chloroform; D004247:DNA / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005821:Genetic Techniques; D006146:Guanidines; D006863:Hydrogen-Ion Concentration; D010636:Phenols; D010957:Plasmids; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification*; D013698:Templates, Genetic; D013861:Thiocyanates; D014158:Transcription, Genetic
D006146:Guanidines; D010636:Phenols; D013861:Thiocyanates; C054436:guanidine thiocyanate; D012313:RNA; D002725:Chloroform; D004247:DNA