Determination of the axial ratio of globular proteins in aqueous solution using viscometric measurements.
The paper presents the results of viscosity determinations on aqueous solutions of several globular proteins in a wide range of concentrations. On the basis of these measurements a general formula connecting the relative viscosity with concentration and axial ratio of the dissolved proteins was established. By applying the formula the axial ratios of bovine gamma-globulin and horse albumin molecules were calculated.
Monkos
K
K
Department of Biophysics, Silesian Medical Academy, Zabrze, Poland.
Turczynski
B
B
D000418:Albumins / Q000737:chemistry; D000818:Animals; D002417:Cattle; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D008970:Molecular Weight; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D014783:Viscosity; D005719:gamma-Globulins / Q000032:analysis
D000418:Albumins; D006454:Hemoglobins; D011506:Proteins; D012996:Solutions; D005719:gamma-Globulins