Conservation of structure and expression of the c-yes and fyn genes in lower vertebrates.
The src-gene family in mammals and birds consists of 9 closely related protein tyrosine kinases. We have cloned the c-yes and fyn homologues of the src-family from the teleost fish Xiphophorus helleri. Both genes show a high degree of sequence conservation and exhibit all structural motifs diagnostic for functional src-like protein tyrosine kinases. Sequence comparisons revealed three domains (exon 2, exons 3-6, exons 7-12) which evolve at different rates. Both genes exhibit an identical expression pattern, with preferential expression in neural tissues. No transcripts of c-yes were found in liver which is contrary to the situation in higher vertebrates. In malignant melanoma, elevated levels of c-yes and fyn were detected indicating a possible function during secondary steps of tumor progression for src-related tyrosine kinases.
Hannig
G
G
Gene Center, Max-Planck-Institute for Biochemistry, Martinsried, Germany.
Ottilie
S
S
Schartl
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura; D005075:Biological Evolution; D015152:Blotting, Northern; D002645:Chickens; D003532:Cyprinodontiformes; D006801:Humans; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051076:Proto-Oncogene Proteins c-fyn; D051575:Proto-Oncogene Proteins c-yes; D012313:RNA / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D019061:src-Family Kinases
D011518:Proto-Oncogene Proteins; D012313:RNA; D011505:Protein-Tyrosine Kinases; C494957:FYN protein, human; D051076:Proto-Oncogene Proteins c-fyn; D051575:Proto-Oncogene Proteins c-yes; D019061:src-Family Kinases