title: Blast colony-forming cells and precursors of colony-forming cells detectable in long-term marrow culture express the same phenotype (CD33- CD34+).
abstract: We have previously shown that CD33- CD34+ human marrow cells are capable of giving rise to colony-forming cells (CFC) in long-term marrow culture (LTMC) but are mainly depleted of progenitors that directly form colonies in semi-solid media. In contrast, the CD33+ CD34+ population contains most of the CFC but not the precursors of these cells detectable in LTMC. The purpose of the present study was to determine if a form of CFC with self-renewal potential, the blast CFC, is contained within the CD33+ CD34+ or CD33- CD34+ population. The results demonstrate that blast CFC segregate within the CD33- population, representing a distinct progenitor population that may be analogous to progenitors for CFC detectable in LTMC.
authors.affiliation: Fred Hutchinson Cancer Research Center, Department of Pediatric Oncology, Seattle, WA 98104.
authors.forename: I D
authors.initials: ID
authors.lastname: Bernstein
authors.forename: A G
authors.initials: AG
authors.lastname: Leary
authors.forename: R G
authors.initials: RG
authors.lastname: Andrews
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
mesh_terms: D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D006098:Granulocytes / Q000166:cytology / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D063268:Sialic Acid Binding Ig-like Lectin 3
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; D063268:Sialic Acid Binding Ig-like Lectin 3