title: Induction of antibodies to canine herpesvirus in mice by immunization with anti-idiotypic antibodies.
abstract: Anti-idiotypic antibodies (anti-Id Abs) were produced in rabbits after inoculation with two mouse monoclonal antibodies (mAbs) directed against canine herpesvirus (CHV) glycoproteins (gps). One of the mAbs, 12H11, was directed against an epitope on gp 145/112 of CHV which induced virus neutralizing (VN) antibodies and against a cross-reacting epitope on the gp 143/108 of feline herpes-virus type 1 (FHV-1). The other mAb, 11F7, was directed against epitopes on CHV gp47 which induce VN and hemagglutination-inhibition (HAI) antibodies. Using VN-inhibition and HAI-inhibition assays with CHV and FHV-1, the anti-Id Abs obviously inhibited the activities of autologous mAbs, suggesting that anti-Id Abs mimic the epitopes of CHV gp 145/112 or FHV-1 gp 143/108 and CHV gp47 by binding the anti-combining site of the mAbs. These anti-Id Abs, when injected into mice, elicited specific CHV-neutralizing and HAI antibody responses, and one of them also elicited a specific FHV-1-neutralizing antibody response. These data supported the idea that immunization with anti-Id Ab can induce specific VN antibody response, as has been theorized by other workers.
authors.affiliation: Department of Veterinary, Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: X N
authors.initials: XN
authors.lastname: Xuan
authors.forename: J A
authors.initials: JA
authors.lastname: Limcumpao
authors.forename: T
authors.initials: T
authors.lastname: Horimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Tohya
authors.forename: M
authors.initials: M
authors.lastname: Azetaka
authors.forename: E
authors.initials: E
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000918:Antibody Specificity; D003429:Cross Reactions; D004285:Dogs; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006564:Herpesviridae / Q000276:immunology*; D007114:Immunization / Q000662:veterinary; D051379:Mice; D009500:Neutralization Tests; D011817:Rabbits
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D006023:Glycoproteins