Tyrosine hydroxylase and galanin mRNA levels in locus coeruleus neurons are increased following reserpine administration.
The neuropeptide galanin coexists in 80-90% of the norepinephrine-containing neurons in the locus coeruleus. In situ hybridization histochemistry was used to examine the effects of reserpine treatment or swim stress on tyrosine hydroxylase and galanin mRNA concentrations in locus coeruleus neurons. Reserpine administration significantly increased tyrosine hydroxylase and galanin mRNA levels in the locus coeruleus. The reserpine-induced increase in tyrosine hydroxylase mRNA was significantly correlated with the reserpine-induced increase in galanin mRNA. Three consecutive days of swim stress did not significantly alter either tyrosine hydroxylase or galanin mRNA concentrations in the locus coeruleus. These data suggest that both tyrosine hydroxylase and galanin gene expression in locus coeruleus neurons may be regulated by a reserpine-sensitive mechanism.
Austin
M C
MC
Clinical Neuroscience Branch, National Institute of Mental Health, Bethesda, Maryland 20892.
Cottingham
S L
SL
Paul
S M
SM
Crawley
J N
JN
D000818:Animals; D019004:Galanin; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D006651:Histocytochemistry; D008125:Locus Coeruleus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012110:Reserpine / Q000494:pharmacology*; D013312:Stress, Physiological / Q000378:metabolism; D013550:Swimming; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics* / Q000378:metabolism
D010455:Peptides; D012333:RNA, Messenger; D019004:Galanin; D012110:Reserpine; D014446:Tyrosine 3-Monooxygenase