title: Decrease in cytokine production by HIV-infected macrophages in response to LPS-mediated activation.
abstract: The capacity of human monocytes/macrophages (M/M) infected with a human immunodeficiency virus-1 (HIV-1) isolate to produce several immunomodulating cytokines including interleukin-1 alpha (IL-1 alpha), IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), IL-6, IL-8, and macrophage chemoattractant and activating factor (MCAF) was examined. Although HIV infection itself induced significant increases in the level of mRNAs for IL-1 beta, TNF-alpha, IL-6, and IL-8, the levels of lipopolysaccharide (LPS)-induced mRNAs for IL-1 alpha, IL-1 beta, TNF-alpha, IL-6, IL-8, and MCAF were decreased over those of uninfected LPS-stimulated cells. In addition, HIV-infected M/M produced lower amounts of IL-8 protein, as measured by radioimmunoassay over an 18-day culture period. These results suggest that HIV infection generally suppresses the LPS-inducible cytokine production in human M/M. The impact of the role of these cytokines in the immunity and pathogenesis of HIV-1 infection is discussed.
authors.affiliation: Laboratory of Biochemical Physiology, PRI/DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702-1201.
authors.forename: W P
authors.initials: WP
authors.lastname: Tsai
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: P L
authors.initials: PL
authors.lastname: Nara
authors.forename: Y D
authors.initials: YD
authors.lastname: Kuang
authors.forename: S
authors.initials: S
authors.lastname: Conley
authors.forename: B Q
authors.initials: BQ
authors.lastname: Li
authors.forename: H F
authors.initials: HF
authors.lastname: Kung
authors.forename: K
authors.initials: K
authors.lastname: Matsushima
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood*; D015152:Blotting, Northern; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008262:Macrophage Activation / Q000187:drug effects*; D009000:Monocytes / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000235:genetics
chemical_list: D016207:Cytokines; D012313:RNA