Expression of interleukin-6 and interleukin-6 receptor in Hodgkin's disease.
Interleukin-6 (IL-6) is a multipotent lymphokine that can mediate differentiation of B cells into Ig-secreting cells, stimulate the growth of plasmacytomas, hybridomas, and T cells, and induce acute-phase proteins in liver cells. It has been suggested that IL-6 is involved in the pathogenesis of several diseases by autocrine or paracrine pathways. To examine whether IL-6 is possibly involved in the pathophysiology of Hodgkin's disease (HD), we analyzed the expression of IL-6 and IL-6 receptor mRNA and protein in cell lines and primary specimens from patients with HD. IL-6-specific transcripts were detected in three of six HD-derived cell lines by Northern blot analysis. In the culture supernatants of four HD-derived cell lines, IL-6 was detected by radioimmunoassay. Biologic activity of IL-6 was confirmed by proliferation of an IL-6-dependent cell line. In situ hybridization experiments showed IL-6-specific transcripts in Hodgkin (H) and Reed-Sternberg (RS) cells in primary tissues of two patients. In addition, mRNAs specific for the IL-6 receptor were detected in five HD-derived cell lines. Immunostaining experiments showed expression of IL-6 receptor molecules on H and RS cells in 8 of 16 cases with HD. Thus, our data suggest that IL-6 might be involved in the pathophysiology of HD.
Jücker
M
M
I. Medizinische Klinik, Universität Köln, Germany.
Abts
H
H
Li
W
W
Schindler
R
R
Merz
H
H
Günther
A
A
von Kalle
C
C
Schaadt
M
M
Diamantstein
T
T
Feller
A C
AC
D000911:Antibodies, Monoclonal; D001681:Biological Assay; D002460:Cell Line; D015870:Gene Expression; D006689:Hodgkin Disease / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis / Q000235:genetics*; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D011863:Radioimmunoassay; D011971:Receptors, Immunologic / Q000032:analysis / Q000235:genetics*; D019947:Receptors, Interleukin-6
D000911:Antibodies, Monoclonal; D015850:Interleukin-6; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6; D011061:Poly A; D012313:RNA