title: Influence of high cholesterol feeding on the pattern and progression of experimental cerebral ischemia.
abstract: The aim of our research was to study if cholesterol feeding might affect the ischemic changes in the vessels surrounding infarction foci in Sephadex G-75-induced cerebral ischemia model (SG-75). One hundred-twenty-four rabbits were divided as follows: group I was given standard food for 5 weeks; group II: as group I and then injected with SG-75; group III: standard food plus 1% cholesterol for 5 weeks; and group IV: as group III and then injected with SG-75. Rabbits were sacrificed 3 h, 6 h and 2, 5 and 7 days after ischemia had occurred. Vessels surrounding infarction foci (SIF) were identified by using a 6% carbon perfusion. Samples were examined by light microscopy and transmission electron microscopy (TEM). The occurrence of hemorrhagic infarction (HI) showed a clear time/course increase in group II whereas a decrease after 2 days in group IV was observed. The rate of HI was 40% and 20% in group II and IV, respectively. SIF vessels showed red blood cells leakage in group II, whereas multiple platelet thrombi appeared in group IV. This phenomenon caused a more extensive ischemic damage, when compared to group II. By making use of a widely employed model of high cholesterol diet and of a more physiological model of cerebral ischemia devised by us, we have provided the evidence that the hypercholesterolemia-induced changes in the SIF vessels strongly affect the pattern and progression of cerebral ischemia.
authors.affiliation: Norman Bethune University, Department of Internal Medicine, Changchun, China.
authors.forename: D H
authors.initials: DH
authors.lastname: Chui
authors.forename: J
authors.initials: J
authors.lastname: Marotta
authors.forename: J S
authors.initials: JS
authors.lastname: Zang
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: M L
authors.initials: ML
authors.lastname: Rao
authors.forename: D S
authors.initials: DS
authors.lastname: Liu
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000473:pathology; D002545:Brain Ischemia / Q000139:chemically induced / Q000150:complications* / Q000473:pathology; D002544:Cerebral Infarction / Q000209:etiology / Q000473:pathology; D002791:Cholesterol, Dietary / Q000378:metabolism*; D003911:Dextrans / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D011817:Rabbits
chemical_list: D002791:Cholesterol, Dietary; D003911:Dextrans; C025614:sephadex