Saccharide-protein covalent conjugates: immunochemical characterization of Citrobacter 036 core oligosaccharide-tetanus toxoid conjugates.
Core oligosaccharides (complete and incomplete) isolated from Citrobacter 036 lipopolysaccharide were covalently conjugated with tetanus toxoid. Serological examination of the Citrobacter 036 core oligosaccharide-tetanus toxoid conjugates showed that they are strong immunogens. The monospecific anti-conjugate sera prepared by immunization of rabbits, were used to study the antigenic relations between lipopolysaccharide core regions of 8 strains of Citrobacter. Immunoelectrophoresis, immunoblotting and quantitative microprecipitation were performed in the experiments.
Lugowski
C
C
Laboratory of Microbial Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Kułakowska
M
M
Romanowska
E
E
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002954:Citrobacter / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes; D007106:Immune Sera; D015151:Immunoblotting; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology*; D011233:Precipitin Tests; D011817:Rabbits; D013745:Tetanus Toxoid / Q000737:chemistry / Q000276:immunology*
D000939:Epitopes; D007106:Immune Sera; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D013745:Tetanus Toxoid