Effects of hyperbaric oxygen and a perfluorooctylbromide emulsion on the radiation responses of tumors and normal tissues in rodents.
Perfluorochemical emulsions are being examined in many laboratory and clinical studies as possible adjuncts to radiotherapy and chemotherapy. The studies reported here examine the clinical potential of hyperbaric oxygen (HBO) in combination with a highly concentrated perfluorochemical emulsion (Oxygent) containing 100% w/v perfluorooctylbromide (PFOB). HBO alone produced only a small improvement in the radiation response of BA1112 tumors in WAG/rij rats, while regimens combining HBO with Oxygent produced much greater radiation sensitization. A sham emulsion, formulated without the O2-carrying PFOB, did not alter the radiation response of the tumors in comparison with that seen with HBO alone. Neither HBO nor Oxygent plus HBO altered the radiosensitivity of bone marrow progenitor cells in BALB/c mice. HBO alone augmented skin reactions in BALB/c mice, but addition of Oxygent did not alter the skin reactions in comparison to those seen with HBO alone. Regimens combining Oxygent with HBO selectively increased the radiation sensitivity of tumors relative to normal tissues, thereby enhancing the therapeutic ratio. These results support the potential usefulness of perfluorochemical emulsions and HBO in clinical radiation therapy.
Rockwell
S
S
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06510-8040.
Irvin
C G
CG
Kelley
M
M
Hughes
C S
CS
Yabuki
H
H
Porter
E
E
Fischer
J J
JJ
D000818:Animals; D003114:Colony-Forming Units Assay; D004655:Emulsions; D005260:Female; D005466:Fluorocarbons / Q000494:pharmacology*; D006098:Granulocytes; D006842:Hydrocarbons, Brominated; D006931:Hyperbaric Oxygenation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D012208:Rhabdomyosarcoma / Q000532:radiotherapy*; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D004655:Emulsions; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D011838:Radiation-Sensitizing Agents; C003072:perflubron