Regeneration of adsorbed and covalently immobilized antibodies on solid phases for immunoassay.
A technique for the reproducible re-use of antibody-coated solid phases for immunoassays is described. The method based on the dissociation of the antigen-antibody complexes. Two different procedures, using glycine buffer (pH 2.3) or ethanolamine, were developed. More than ten immunoassay cycles can be realized with the same antibody-coated microtitre plates. These procedures were tested with competitive and sandwich immunoassays, monoclonal and polyclonal antibodies, and a commercial immunoassay kit.
Ilchmann
D
D
Forschungsinstitut für Medizinische Diagnostik, Dresden, DDR.
Helbig
D
D
Göhler
H
H
Stopsack
M
M
Thiele
H J
HJ
Hubl
W
W
D000327:Adsorption; D000469:Alkaline Phosphatase / Q000032:analysis* / Q000276:immunology; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007202:Indicators and Reagents; D011374:Progesterone / Q000032:analysis* / Q000276:immunology; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007202:Indicators and Reagents; D000509:alpha-Fetoproteins; D011374:Progesterone; D000469:Alkaline Phosphatase