Effect of light on the NADPH-protochlorophyllide oxidoreductase of Arabidopsis thaliana.
A cDNA encoding the NADPH-protochlorophyllide oxidoreductase (Pchlide reductase) of Arabidopsis thaliana has been isolated and sequenced. The cDNA contains the complete reading frame for the precursor of the Pchlide reductase. The deduced amino acid sequence of the Arabidopsis enzyme closely resembles the corresponding sequences of barley and oat. The cDNA has been used as a template for the synthesis of the enzyme protein in Escherichia coli. An antiserum was raised against this enzyme protein and both the antiserum and the cDNA were used as experimental tools to study the effects of light on the Pchlide reductase in A. thaliana. When etiolated seedlings of Arabidopsis were exposed to light the enzyme activity and the concentration of the enzyme protein rapidly declined. Similar light effects have been described previously for other angiosperms. In contrast to most of these species, however, in Arabidopsis only minor changes in Pchlide reductase mRNA content could be observed when etiolated seedlings were exposed to light.
Benli
M
M
Botanisches Institut der Christian-Albrechts-Universität Kiel, Germany.
Schulz
R
R
Apel
K
K
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005786:Gene Expression Regulation / Q000528:radiation effects; D017343:Genes, Plant / Q000528:radiation effects*; D001467:Hordeum / Q000201:enzymology / Q000235:genetics; D008027:Light; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000528:radiation effects*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000528:radiation effects; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D011994:Recombinant Proteins; D011061:Poly A; D012313:RNA; D004247:DNA; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C023886:protochlorophyllide reductase