title: Characterization of murine monoclonal antibodies that recognize defined epitopes of pertussis toxin and neutralize its toxic effect on Chinese hamster ovary cells.
abstract: Three murine monoclonal antibodies (MAb), E19, E205, and E251, raised against pertussis toxin reacted in Western blots (immunoblots) with the S1, S4, and S2-S3 subunits, respectively, and neutralized the Chinese hamster ovary cell-clustering activity of pertussis toxin. MAb E251 recognized a linear synthetic peptide corresponding to amino acids 107 to 120 of the S2 subunit, suggesting a role for this region in receptor binding.
authors.affiliation: Department of Microbiology, GBF-National Research Centre for Biotechnology, Braunschweig, Germany.
authors.forename: M J
authors.initials: MJ
authors.lastname: Walker
authors.forename: J
authors.initials: J
authors.lastname: Wehland
authors.forename: K N
authors.initials: KN
authors.lastname: Timmis
authors.forename: B
authors.initials: B
authors.lastname: Raupach
authors.forename: M A
authors.initials: MA
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000992:Antitoxins / Q000276:immunology*; D015153:Blotting, Western; D016466:CHO Cells; D006224:Cricetinae; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D066298:In Vitro Techniques; D051379:Mice; D037342:Pertussis Toxin; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000276:immunology* / Q000633:toxicity
chemical_list: D000911:Antibodies, Monoclonal; D000992:Antitoxins; D000939:Epitopes; D010566:Virulence Factors, Bordetella; D011065:Poly(ADP-ribose) Polymerases; D037342:Pertussis Toxin