title: Substrate response in acid phosphatase activity of Pseudomonas pseudomallei and Pseudomonas cepacia, with special reference to tyrosine phosphatase.
abstract: The substrate response in acid phosphatase activity of Pseudomonas pseudomallei and Pseudomonas cepacia was examined with different phosphate esters including hexose phosphates and phosphoaminoacids in a whole cell assay system. The enzymatic activity against each substrate was evaluated in terms of percent activity to that against para-nitrophenyl phosphate set as 100. A remarkable finding was that the phosphatase reaction was the highest with phosphotyrosine or phosphoserine as substrate showing 180% activity. This tyrosine phosphatase activity was resistant to heating at 60 C for 20 min and inhibited greatly by 0.1% ZnCl2. Pseudomonas cepacia showed the same pattern of substrate response and the same characteristics of tyrosine phosphatase activity.
authors.affiliation: National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand.
authors.forename: K
authors.initials: K
authors.lastname: Kanai
authors.forename: E
authors.initials: E
authors.lastname: Kondo
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D016956:Burkholderia cepacia / Q000201:enzymology*; D016957:Burkholderia pseudomallei / Q000201:enzymology*; D002712:Chlorides / Q000494:pharmacology; D006358:Hot Temperature / Q000009:adverse effects; D006801:Humans; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013379:Substrate Specificity; D015032:Zinc / Q000494:pharmacology; D017967:Zinc Compounds
chemical_list: D002712:Chlorides; D017967:Zinc Compounds; C016837:zinc chloride; D000135:Acid Phosphatase; D017027:Protein Tyrosine Phosphatases; D015032:Zinc