Molecular cloning and expression of biologically active human glia maturation factor-beta.
Glia maturation factor-beta, a protein found in the brains of all vertebrates thus far examined, appears to play a role in the differentiation, maintenance, and regeneration of the nervous system. Using oligonucleotide probes based on the sequences of three tryptic peptides derived from bovine glia maturation factor-beta, we screened a human brainstem cDNA library in lambda gt11. A 0.7-kb clone was isolated, sequenced in its entirety, and found to encode a polypeptide of 142 amino acids which contained regions identical to the three bovine peptides. This polypeptide, human recombinant glia maturation factor-beta, has been expressed in Escherichia coli and found to possess structural characteristics and biological activity indistinguishable from those of the native bovine protein.
Kaplan
R
R
Department of Neurology, Division of Neurochemistry and Neurobiology, University of Iowa College of Medicine, Iowa City.
Zaheer
A
A
Jaye
M
M
Lim
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001933:Brain Stem / Q000502:physiology*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D015723:Gene Library; D020904:Glia Maturation Factor; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D015345:Oligonucleotide Probes; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology
D020904:Glia Maturation Factor; D006133:Growth Substances; D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D012313:RNA