title: Retrograde axonal transport of locally synthesized phosphoinositides in the rat sciatic nerve.
abstract: Although autoradiography has demonstrated local incorporation of [3H]inositol into axonal phospholipids after intraneural injection, retrograde axonal transport of phosphatidylinositol has only been demonstrated after injection of lipid precursor into the cell body regions (L4 and L5 dorsal root ganglia) of the sciatic nerve. We now report the retrograde axonal transport of inositol phospholipids synthesized locally in the axons. Following microinjection of myo-[3H]inositol into the rat sciatic nerve (50-55 mm distal to L4 and L5 dorsal root ganglia), a time-dependent accumulation of 3H label occurred in the dorsal root ganglia ipsilateral to the injection site. The ratio of dpm present in the ipsilateral dorsal root ganglia to that in the contralateral dorsal root ganglia was not significantly different from unity between 2 and 8 h following isotope injection but increased to 10-12-fold between 24 and 72 h following precursor injection. By 24 h following precursor injection, the ipsilateral/contralateral ratio of the water-soluble label in the dorsal root ganglia still remained approximately 1.0, whereas the corresponding ratio in the chloroform/methanol-soluble fraction was approximately 20. The time course of appearance of labeled lipids in the ipsilateral dorsal root ganglia after injection of precursor into the nerve at various distances from the dorsal root ganglia indicated a transport rate of at least 5 mm/h. Accumulation of label in the dorsal root ganglia could be prevented by intraneural injection of colchicine or ligation of the sciatic nerve between the dorsal root ganglia and the isotope injection site. These results demonstrate that inositol phospholipids synthesized locally in the sciatic nerve are retrogradely transported back to the nerve cell bodies located in the dorsal root ganglia.
authors.affiliation: Cellular and Molecular Toxicology Branch, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711.
authors.forename: S
authors.initials: S
authors.lastname: Padilla
authors.forename: C N
authors.initials: CN
authors.lastname: Pope
mesh_terms: D000818:Animals; D001370:Axonal Transport; D007839:Functional Laterality; D005727:Ganglia, Spinal / Q000502:physiology; D007294:Inositol / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008959:Models, Neurological; D010716:Phosphatidylinositols / Q000096:biosynthesis / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000502:physiology*; D013116:Spinal Cord / Q000502:physiology; D013997:Time Factors; D014316:Tritium
chemical_list: D010716:Phosphatidylinositols; D014316:Tritium; D007294:Inositol