title: Monoclonal antibodies against human placental glutathione transferase (class pi).
abstract: Five monoclonal antibodies (MAbs) were produced in a mouse hybridoma system against human placental glutathione transferase (GST pi). Four of these monoclonal antibodies, named 461 to 464, were of immunoglobulin G class, whereas the monoclonal antibody 465 was of IgA class. All these MAbs specifically recognized the glutathione transferase from human placenta (class pi) showing no cross reactivity against the basic and the neutral forms of GST from human liver. When each MAb was incubated with the GST pi, no inhibition of enzymatic activity towards 1-chloro-2,4-dinitrobenzene was observed except for MAb 465 which showed a slight inhibition to a serial dilution of 1:128.
authors.affiliation: Department of Biology, 2nd University of Rome Tor Vergata, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Massoud
authors.forename: M
authors.initials: M
authors.lastname: Lo Bello
authors.forename: E
authors.initials: E
authors.lastname: De Stefano
authors.forename: A
authors.initials: A
authors.lastname: Molino
authors.forename: D
authors.initials: D
authors.lastname: Zelaschi
authors.forename: G
authors.initials: G
authors.lastname: Federici
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D000918:Antibody Specificity; D001665:Binding Sites; D000939:Epitopes; D005260:Female; D005982:Glutathione Transferase / Q000145:classification / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D051379:Mice; D010920:Placenta / Q000276:immunology; D011247:Pregnancy
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005982:Glutathione Transferase