title: Colocalization of immunoreactive oxytocin, vasopressin and interleukin-1 in human thymic epithelial neuroendocrine cells.
abstract: Monoclonal antibodies to oxytocin (OT) and vasopressin (VP) revealed some positively staining stromal cells in the subcapsular cortex and in the medulla of the human thymus. We further demonstrated that these cells are a subset of epithelial endocrine cells and also contain immunoreactive interleukin-1 together with the neuropeptides. In addition, the thymic cells stained by monoclonal antibodies directed to the cyclic part of oxytocin or vasopressin also contained some immunoreactive neurophysins. These data support the concept of intrathymic synthesis of neurohypophyseal-like peptides fitting the hypothalamic model. However, we observed that, contrary to the situation in the brain, OT- and VP-like peptides colocalized in the same thymic cells. Furthermore, one monoclonal antibody, specific for the tail part of oxytocin, did not label thymic cells. Therefore, thymic neuropeptide(s) could be related to, but distinct from, authentic OT and VP. These observations suggest some molecular differences between hypothalamic and thymic oxytocin biosynthetic pathways which need to be further investigated.
authors.affiliation: INSERM, U-308, Faculty of Medicine, Vandoeuvre-les-Nancy, France.
authors.forename: F
authors.initials: F
authors.lastname: Robert
authors.forename: V
authors.initials: V
authors.lastname: Geenen
authors.forename: J
authors.initials: J
authors.lastname: Schoenen
authors.forename: E
authors.initials: E
authors.lastname: Burgeon
authors.forename: D
authors.initials: D
authors.lastname: De Groote
authors.forename: M P
authors.initials: MP
authors.lastname: Defresne
authors.forename: J J
authors.initials: JJ
authors.lastname: Legros
authors.forename: P
authors.initials: P
authors.lastname: Franchimont
mesh_terms: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D004847:Epithelial Cells; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007375:Interleukin-1 / Q000032:analysis*; D007633:Keratins / Q000032:analysis; D015213:Neuroimmunomodulation / Q000502:physiology*; D009481:Neurophysins / Q000032:analysis* / Q000276:immunology; D009490:Neurosecretory Systems / Q000737:chemistry*; D010121:Oxytocin / Q000235:genetics / Q000276:immunology; D013950:Thymus Gland / Q000737:chemistry / Q000166:cytology*; D014667:Vasopressins / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007375:Interleukin-1; D009481:Neurophysins; D014667:Vasopressins; D010121:Oxytocin; D007633:Keratins