Unique 3'-untranslated sequence of insulin-like growth factor-I isolated from human placenta.
A cDNA clone of 525 bp corresponding to the 3'-untranslated region of insulin-like growth factor-I was isolated from a human placenta library. The sequence of this clone extended 200 nucleotides downstream from the previously reported 3'-end of IGF-IA cDNA, indicating the existence of IGF-IA transcripts having an even larger 3'-untranslated region. By using this clone for RNA transfer blot hybridization, it was shown that this longer 3'-untranslated region is included in the 7.5- and 5.0-kb transcripts, but not in the 1.1- and 0.9-kb transcripts. It is also apparent that transcripts bearing the extended 3'-untranslated sequence are highly expressed in human placenta.
Daimon
M
M
Department of Reproductive Biology, Case Western Reserve University School of Medicine, Cleveland, Ohio.
Wang
C Y
CY
Johnson
T R
TR
Ilan
J
J
Ilan
J
J
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism*; D011061:Poly A / Q000235:genetics; D011247:Pregnancy; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012343:RNA, Transfer / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D007334:Insulin-Like Growth Factor I; D004247:DNA; D012343:RNA, Transfer