Calcium signal and histamine secretion induced by aggregation of immunoglobulin E receptors in rat basophilic leukemia (RBL-2H3) cells.
The changes in cytosolic Ca concentration in rat basophilic leukemia cells (RBL-2H3) were determined using a fluorescent Ca probe, fura-2, after aggregation of IgE receptors. By means of a superfusion system, both histamine secretion and changes in cytosolic Ca concentration were determined simultaneously. In addition, the microscopic observation revealed the oscillatory Ca signal after stimulation with an antigen.
Maeyama
K
K
Department of Pharmacology I, Tohoku University School of Medicine, Sendai, Japan.
Yagi
S
S
Sasaki
M
M
Sakurai
E
E
Watanabe
T
T
D000818:Animals; D000941:Antigens / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004140:Dinitrophenols / Q000276:immunology; D006636:Histamine Release; D015471:Leukemia, Basophilic, Acute / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D011961:Receptors, Fc / Q000378:metabolism*; D017455:Receptors, IgE; D013189:SRS-A / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000276:immunology; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D000941:Antigens; D000944:Antigens, Differentiation, B-Lymphocyte; D004140:Dinitrophenols; D011961:Receptors, Fc; D017455:Receptors, IgE; D013189:SRS-A; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D002118:Calcium