[An interferon inhibitor in regulating the activity of human natural killer cells].
The action of mouse serum interferon--alpha/beta (IFN) at the dose of 100 U/ml, of its inhibitor (I) at the dose of 8 U/ml as well as of their combination with the above doses on sensitivities of mouse target cells (TC) of sensitive to IFN line L 929 and resistant to the one line MCB in natural cytotoxic reaction was studied. Cytotoxic activity of human natural killer cells was detected in 14 hrs cytotoxic test using 3H-uridine for labelling of TC. IFN, I, and IFN+I were added to cell cultures for 24 hrs at 37 degrees C with following removing of preparations. It has been shown that I abolished protective effect of IFN on TC L 929 whereas the one possessed the protective action on TC MCB in natural cytotoxic reaction. These data confirmed a suggestion about immunoregulatory properties of I which displayed in abolition or realization of protective effect on TC in natural cytotoxic reaction in dependence on initial sensitivity of TC to antiviral IFN action.
Cheknev
S B
SB
Narovlianskiĭ
Amchenkova
A M
AM
Ershov
F I
FI
D000818:Animals; D003602:Cytotoxicity, Immunologic; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D016899:Interferon-beta / Q000494:pharmacology; D007372:Interferons / Q000037:antagonists & inhibitors*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D014529:Uridine / Q000378:metabolism
D016898:Interferon-alpha; D016899:Interferon-beta; D007372:Interferons; D014529:Uridine
Ingibitor deĭstviia interferona v reguliatsii aktivnosti estestvennykh killerov cheloveka.