title: Acridine orange staining in early diagnosis of mycotic keratitis.
abstract: We have assessed the comparative sensitivities of acridine orange staining and 10% potassium hydroxide wet mount in the examination of corneal scrapings from one hundred and sixty-one clinically suspected cases of mycotic keratitis. The sensitivity of acridine orange was found to be 76% as compared to that of potassium hydroxide (65%). The predictive value for culture result was also seen to be higher. Although both the values were not statistically significant (P greater than 0.05), acridine orange staining was a rapid and simple technique with an added advantage of detecting inflammatory cells in corneal scrapings.
authors.affiliation: Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
authors.forename: R
authors.initials: R
authors.lastname: Kanungo
authors.forename: R
authors.initials: R
authors.lastname: Srinivasan
authors.forename: R S
authors.initials: RS
authors.lastname: Rao
mesh_terms: D000165:Acridine Orange; D015715:Corneal Edema / Q000382:microbiology; D015821:Eye Infections, Fungal / Q000175:diagnosis* / Q000382:microbiology; D005658:Fungi / Q000302:isolation & purification; D006801:Humans; D006878:Hydroxides; D008856:Microscopy, Fluorescence; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D011188:Potassium; D017680:Potassium Compounds; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D006878:Hydroxides; D017680:Potassium Compounds; D000165:Acridine Orange; D011188:Potassium; C029943:potassium hydroxide