title: Synthetic peptides in HIV antibody screening and typing.
abstract: We have defined continuous native epitopes of HIV proteins by using a systematic epitope-scanning technology. We have demonstrated that there is a highly immunoreactive continuous native epitope region in the transmembrane protein gp41 of HIV-1 that is immunoreactive with all studied HIV-1 antibody-positive sera. The corresponding region in HIV-2 gp34 behaves similarly. There is a clear difference, however, between HIV type 1 and type 2 transmembrane proteins in the number of highly immunoreactive regions, when presented properly as synthetic antigens in solid-phase EIA, can provide tests unusually suitable for early and reliable diagnosis of HIV-1 and HIV-2 infections and for type-specific distinction of the two types of HIV infections.
authors.affiliation: Laboratory of Human Retrovirology, School of Medicine, University of Genoa, Italy.
authors.forename: O E
authors.initials: OE
authors.lastname: Varnier
authors.forename: A
authors.initials: A
authors.lastname: Närvänen
authors.forename: M
authors.initials: M
authors.lastname: Korkolainen
authors.forename: F
authors.initials: F
authors.lastname: Lillo
authors.forename: S
authors.initials: S
authors.lastname: Kontio
authors.forename: J
authors.initials: J
authors.lastname: Elm
authors.forename: J
authors.initials: J
authors.lastname: Suni
authors.forename: A
authors.initials: A
authors.lastname: Vaheri
authors.forename: M L
authors.initials: ML
authors.lastname: Huhtala
mesh_terms: D000939:Epitopes; D015483:HIV Antibodies / Q000032:analysis* / Q000145:classification; D015658:HIV Infections / Q000145:classification / Q000175:diagnosis; D006801:Humans; D010455:Peptides / Q000138:chemical synthesis* / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000939:Epitopes; D015483:HIV Antibodies; D010455:Peptides; D014764:Viral Proteins