title: Variant cDNAs encoding proteins similar to the alpha subunit of chicken CapZ.
abstract: Chicken adult muscle and liver cDNA libraries were screened with a cDNA, alpha 1, previously isolated from a chicken embryo library by screening with antibodies against the alpha subunit of chicken CapZ. cDNAs with a new coding region, called alpha 2, were found in addition to ones with the alpha 1 coding region. alpha 2 predicts a protein sequence that matches exactly the N-terminal sequence of 5 peptides prepared from CapZ alpha purified from chicken muscle, while the protein sequence predicted by alpha 1 matches the peptides well, but not exactly. The predicted protein sequences of alpha 1 and alpha 2 are very similar to each other, and they are similar to those of the alpha subunit of capping protein from Dictyostelium [Hartmann et al., J. Biol. Chem. 163:5254-5254, 1989] and an actin-binding protein from Xenopus [Ankenbauer et al., Nature 342:822-824, 1989]. Other conserved features of the predicted primary and secondary structures are noted. Chicken alpha 1 and alpha 2 are transcribed in all of 7 adult chicken muscle and non-muscle tissues in comparable amounts by Northern analysis. alpha 2 has four poly(A)+RNA transcripts, one of which is rare in liver. alpha 1 has two transcripts. alpha 1 and alpha 2 are encoded by different single-copy genes by Southern analysis of chicken genomic DNA.
authors.affiliation: Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri.
authors.forename: J A
authors.initials: JA
authors.lastname: Cooper
authors.forename: J E
authors.initials: JE
authors.lastname: Caldwell
authors.forename: D J
authors.initials: DJ
authors.lastname: Gattermeir
authors.forename: M A
authors.initials: MA
authors.lastname: Torres
authors.forename: J F
authors.initials: JF
authors.lastname: Amatruda
authors.forename: J F
authors.initials: JF
authors.lastname: Casella
mesh_terms: D051339:Actin Depolymerizing Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D051347:CapZ Actin Capping Protein; D002645:Chickens / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D051345:Destrin; D004023:Dictyostelium / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000378:metabolism; D011061:Poly A / Q000032:analysis / Q000235:genetics; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014981:Xenopus / Q000235:genetics
chemical_list: D051339:Actin Depolymerizing Factors; D051347:CapZ Actin Capping Protein; D015342:DNA Probes; D051345:Destrin; D008840:Microfilament Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA