[Direct breaks in a single-stranded DNA fragment by a bleomycin-derived oligonucleotide].
A method for coupling bleomycin to oligonucleotides is suggested. The reaction was carried out between the amino group of the spermidine residue of the bleomycin A5 Cu(II)-complex (Cu(II)Blm-RH) and the 5'-phosphate group of the oligonucleotide pd(CCAAACA) (I) activated with a mixture of triphenylphosphine and 2,2'-dipyridyldisulphide in the presence of 4-N,N-dimethylaminopyridine-1-oxide. The resultant compound (Ia) (yield 70%) forms more stable complementary complexes than the parent oligonucleotide (delta Tm = 11 degrees C). When Cu(II) ion was removed from (Ia), compound (Ib) formed which effectively (80%) cleaved pd(TGTTTGGCGAAGGA). Neither pd(TCCTTCG) nor the oligonucleotide tail of the reagent (Ib) were destroyed under the cleavage conditions. Free Blm-RH and bleomycin bound in the reagent (Ib) damage different regions of the target.
Godovikova
T S
TS
Zarytova
V F
VF
Sergeev
D S
DS
D001483:Base Sequence; D001761:Bleomycin / Q000494:pharmacology* / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid; D004277:DNA, Single-Stranded / Q000187:drug effects* / Q000378:metabolism; D004586:Electrophoresis; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000378:metabolism*
D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D001761:Bleomycin; C025703:bleomycetin
Priamye razryvy odnotsepochechnogo fragmenta DNK bleomitsinovym proizvodnym oligonukleotida.