Light-induced, GTP-binding protein mediated membrane currents of Xenopus oocytes injected with rhodopsin of cephalopods.
Xenopus oocytes that were injected with rhabdomeric membranes of squid and octopus photoreceptors acquired light sensitivity. The injected oocytes showed a light-induced current having characteristics similar to other G-protein-mediated Cl- currents induced by the activation of other membrane receptors. Pretreatment of the oocytes with pertussis toxin before the injection suppressed the generation of the light-induced current, indicating an ability of cephalopod rhodopsin to cross-react with an endogenous G-protein of Xenopus oocytes.
Ando
H
H
Department of Physiology, School of Medicine, Kobe University, Japan.
Seidou
M
M
Kito
Y
Y
D000818:Animals; D002712:Chlorides / Q000502:physiology; D003429:Cross Reactions; D049832:Decapodiformes; D005260:Female; D019204:GTP-Binding Proteins / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D049831:Octopodiformes; D009865:Oocytes / Q000502:physiology*; D010775:Photic Stimulation; D012243:Rhodopsin / Q000502:physiology*; D013997:Time Factors; D014982:Xenopus laevis
D002712:Chlorides; D007473:Ion Channels; D012243:Rhodopsin; D019204:GTP-Binding Proteins