title: Intratypic variations in neutralizable epitopes among herpes simplex virus type 2 isolates.
abstract: Intratypic variation among 94 isolates of herpes simplex virus type 2 (HSV-2) was investigated using 4 different monoclonal antibodies (MAbs). By neutralization test, these MAbs appeared to be directed to at least 2 distinct epitopes on the viral glycoprotein D (gD), i.e., 6G6.G9 and 6E8.F11 which did not require complement (C-MAb) and gD-105 and gD-110 whose neutralizing activities could be enhanced by complement (C+MAb). The C-MAb pairs each separately could detect significant intratypic variations among the isolates. Whether these variations also existed in the gD epitope(s) recognized by C+MAbs remains to be elucidated. The results suggested that intratypic variation occurred on at least one of the neutralizable (thus related to protective immunity) epitopes on gD of HSV-2.
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Bhattarakosol
authors.forename: C
authors.initials: C
authors.lastname: Yoosook
authors.forename: P
authors.initials: P
authors.lastname: Matangkasombut
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006168:Guinea Pigs; D006558:Herpes Genitalis / Q000382:microbiology; D006801:Humans; D009500:Neutralization Tests; D051381:Rats; D018139:Simplexvirus / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins; C040570:glycoprotein D-herpes simplex virus type 2