title: [Localization of an antigenic determinant of recombinant interleukin-2, recognized by monoclonal antibody 13B1].
abstract: We suggest a simple approach to localization of antigenic determinants for the monoclonal antibody 13B1 raised against recombinant human interleukin-2. The approach is based on the limited trypsin proteolysis, peptide separation by the O'Farrell method and identification of the peptides, interacting with monoclonal antibodies, and comparison of the charge and length of these peptides with the corresponding values of theoretically possible peptides.
authors.forename: V A
authors.initials: VA
authors.lastname: Skrivelis
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Bundulis
authors.forename: A V
authors.initials: AV
authors.lastname: Paniutich
authors.forename: N N
authors.initials: NN
authors.lastname: Voĭtenok
authors.forename: V E
authors.initials: VE
authors.lastname: Lunev
authors.forename: V A
authors.initials: VA
authors.lastname: Nesmeianov
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Tsimanis
authors.forename: E Ia
authors.initials: EIa
authors.lastname: Gren
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology*; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D014357:Trypsin / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007376:Interleukin-2; D011994:Recombinant Proteins; D014357:Trypsin
vernacular_title: Lokalizatsiia antigennoĭ determinanty rekombinantnogo interleĭkina-2, uznavaemoĭ monoklonal'nymi antitelami 13B1.