[Assessment of the reparative capacity of the small intestine in celiac disease in children according to the DNA and RNA contents in enterocyte nuclei].
In 44 children suffering from celiac disease, cytophotometry was used to measure the content of DNA and RNA in enterocyte nuclei in the jejunum mucosa. The measurements were made after the diagnosis was established, 6-8 months and 1-1.5 years after administration of the agliadin diet. In celiac disease children, there was an increase of the content of DNA and RNA in enterocyte nuclei of the intestinal villi and cryptae and lack of gradient in the distribution of the amount of nucleic acids along the length of the villi, which characterized the hyper-regeneration type of small intestinal mucosa atrophy. The recovery of the crypta/villus gradient accompanied by the normalization of the content of nucleic acids, comparable with the analogous indicators of the unchanged mucosa of the small intestine, allows regarding the measurements of the content of DNA and RNA in the nuclei as an index of reparative capacity of enterocytes in children afflicted with celiac disease.
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D002446:Celiac Disease / Q000178:diet therapy / Q000503:physiopathology*; D002648:Child; D002675:Child, Preschool; D003592:Cytophotometry; D004247:DNA / Q000032:analysis*; D006801:Humans; D007223:Infant; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism / Q000503:physiopathology*; D008954:Models, Biological; D012313:RNA / Q000032:analysis*; D012038:Regeneration
D012313:RNA; D004247:DNA
Otsenka reparativnoĭ sposobnosti tonkoĭ kishki pri tseliakii u deteĭ po soderzhaniiu DNK i RNK v iadrakh énterotsitov.