The protein-encoding gene T-urf13 is not edited in maize mitochondria.
RNA editing of T-urf13, a gene specific to the mitochondria of cytoplasmic male-sterile, type-T (cms-T) maize, and an adjacent, cotranscribed gene orf221, have been studied by cDNA sequencing. No editing was detected in 22 cDNA clones. This is the only report of a polypeptide-encoding gene in higher-plant mitochondria that is not edited. T-urf13 may not be edited because it is derived largely from the coding and flanking regions, which are rarely edited, of a ribosomal RNA gene. orf221 is edited; however, the similarity between the predicted amino acid sequences of orf221 in cms-T and normal cytoplasms is not increased.
Ward
G C
GC
Department of Genetics, North Carolina State University, Raleigh 27695-7614.
Levings
C S
CS
D000595:Amino Acid Sequence; D001483:Base Sequence; D017343:Genes, Plant; D016678:Genome; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
D024101:Mitochondrial Proteins; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins; C071393:URF13 protein, Zea mays; D012313:RNA