Novel DNA superstructures formed by telomere-like oligomers.
DNA oligomers containing three or more contiguous guanines form tetrastranded parallel complexes, G4-DNA, in the presence of alkali cations. However, oligomers that have a single multi-guanine motif at their 3' or 5' end, with a guanine as the terminal base, also form higher order products. Thus, the oligomer T8G3T forms a unique G4-DNA product at neutral pH in the presence of Na+, K+, or Rb+; however, its isomeric counterpart T9G3 in K+ or Rb+ generates an additional ladder of products of substantially lower gel mobility. We show that these larger complexes contain, respectively, 8, 12, or 16 distinct strands of oligomer. The octamer structure formed by T9G3 assembles in moderate salt at room temperature and melts around 60 degrees C in 100 mM KCl. Methylation protection experiments suggest a nested head-to-tail superstructure containing two tetraplexes bonded front-to-back via G quartets formed by out-of-register guanines. Naturally occurring chromosomal telomeres, which all have guanines at their 3' termini, may be able to form these superstructures.
Sen
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Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
Gilbert
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W
D001483:Base Sequence; D002412:Cations; D004247:DNA / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D008745:Methylation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D016615:Telomere / Q000737:chemistry*
D002412:Cations; D009841:Oligonucleotides; D006147:Guanine; D004247:DNA