Newly established uterine cervical carcinoma cell line with co-amplification of human papillomavirus DNA and c-myc gene.
A new human tumor cell line, NCC-c-CX-1 (CX-1), was established from a uterine cervical cancer xenografted in nude mice. This cell line harbored approximately 50 to 100 copies of human papillomavirus (HPV) type 18 DNA per haploid genome, and contained about 16-fold-amplified c-myc gene with rearrangement. These genomic alterations found in CX-1 cells were also present in both primary tumor and xenografted tumor. Histopathologically, original and xenografted tumors were poorly differentiated cancer and were characterized by neuroendocrine features such as positive neuron-specific enolase and chromogranin A by immunohistochemistry and abundant neurosecretory-type granules in the cytoplasm by electron microscopy. However, the established cell line had lost the neuroendocrine features. This cervical cancer cell line may be a useful model for studying cervical carcinogenesis, especially the interaction between HPV and c-myc oncogene.
Gotoh
M
M
Pathology Division, National Cancer Center Research Institute, Tokyo.
Nakajima
T
T
Yokota
J
J
Tsunokawa
Y
Y
Terada
M
M
Shimoyama
Y
Y
Teshima
S
S
Hirohashi
S
S
Shimosato
Y
Y
D000328:Adult; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002455:Cell Division; D002460:Cell Line; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D007700:Kinetics; D051379:Mice; D008819:Mice, Nude; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure
D004273:DNA, Neoplasm; D004279:DNA, Viral; D012313:RNA; D010751:Phosphopyruvate Hydratase