Human neuroblastoma cells treated with aluminium express an epitope associated with Alzheimer's disease neurofibrillary tangles.
A number of studies have implicated aluminium as a possible factor in the pathogenesis of Alzheimer's disease (AD). Following an examination of the uptake of aluminium by human neuroblastoma cells in culture, treated with a range of concentrations of aluminium complexed with ethylene-diaminetetra-acetic acid (EDTA), we have now carried out an immunocytochemical study. Using an antibody to phosphorylated tau protein, which reacts specifically with AD neurofibrillary tangles (NFT), we have found that after treatment periods of 16 days to 8 weeks with aluminium-EDTA, the cells show positive staining with this antibody. No such reaction was detected in cells grown in medium alone, nor in aluminium-EDTA-treated cells subjected to the same immunocytochemical procedure but without added primary antibody. Cells grown in medium plus EDTA, which contains a low level of aluminium contamination, showed a slight reaction. Our system may provide a suitable model for studying the early changes which lead to NFT formation.
Guy
S P
SP
Department of Optometry and Vision Sciences, UMIST, Manchester, U.K.
Jones
D
D
Mann
D M
DM
Itzhaki
R F
RF
D000535:Aluminum / Q000633:toxicity*; D000544:Alzheimer Disease / Q000276:immunology / Q000473:pathology*; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000276:immunology; D009447:Neuroblastoma / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000378:metabolism*; D016875:tau Proteins
D000939:Epitopes; D008869:Microtubule-Associated Proteins; D016875:tau Proteins; D000535:Aluminum