title: Centrifugal growth in orthotopic grafts of allogeneic dorsal root ganglia in adult rats: evidence for possible central ingrowth?
abstract: Fetal allogeneic dorsal root ganglion (DRG) transplants from 13-15 day rat embryo's (E13-E15) survived and differentiated when grafted orthopically (within the capsules of the excised 4th and 5th lumbar (L4-L5) ganglia) in adult rats. Survival of grafted neurones was established by prelabeling the grafts with a fluorescent vital dye (DiI) and visualizing the retained fluorescent marker 3 to 9 months later. Simultaneous retrograde tracing using fluorescent tracers applied in the spinal cord and peripheral nerve, respectively, yielded double-labeled dorsal root ganglion neurons, some of which were prelabeled. These findings demonstrate that prelabeled E13-E15 ganglia survive orthopic grafting, organotypically differentiate into mature DRG neurones, and can be double-labeled with fluorescent dyes applied to their peripherally and centrally directed processes. The presence of DiI containing cells which were retrogradely labeled from the spinal cord suggests that fetal (E13-E15) ganglia may have the capability of growing into a mature spinal cord.
authors.affiliation: Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
authors.forename: C M
authors.initials: CM
authors.lastname: Rosario
authors.forename: H
authors.initials: H
authors.lastname: Aldskogius
authors.forename: T
authors.initials: T
authors.lastname: Carlstedt
authors.forename: R L
authors.initials: RL
authors.lastname: Sidman
mesh_terms: D000375:Aging; D000818:Animals; D002232:Carbocyanines; D005260:Female; D016332:Fetal Tissue Transplantation / Q000502:physiology; D005456:Fluorescent Dyes; D005727:Ganglia, Spinal / Q000166:cytology / Q000254:growth & development*; D005865:Gestational Age; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology / Q000254:growth & development*; D014184:Transplantation, Homologous
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C024286:3,3'-dioctadecylindocarbocyanine