Bovine lactoferrin mRNA: sequence, analysis, and expression in the mammary gland.
The mRNA sequence for bovine lactoferrin expressed in the mammary gland was determined by sequencing three over lapping cDNA clones and by direct sequencing of the mRNA. The mRNA (2351 bases) codes for a 708 amino acid protein with a 19 amino acid signal peptide immediately preceding a sequence identical to the N-terminal 40 amino acids reported for bovine lactoferrin. A putative destabilizing sequence (AUUUA) was identified in the 3'-untranslated region. The nucleic acid sequence and deduced amino acid sequence are highly homologous with other transferrin family members. Lactoferrin mRNA concentrations in bovine mammary tissue were quite low two days before parturition and during lactation but were high three days after the cessation of milking, a sharp contrast from the pattern of regulation of the other milk proteins.
Goodman
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J. A. Baker Institute, Cornell University, Ithaca, New York 14853.
Schanbacher
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D015342:DNA Probes / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014168:Transferrin / Q000235:genetics
D015342:DNA Probes; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D014168:Transferrin; D012313:RNA; D007781:Lactoferrin