title: Pathophysiology of Streptococcus pneumoniae otitis media: kinetics of the middle ear biochemical and cytologic host responses.
abstract: Streptococcus pneumoniae is an important bacterial pathogen in the pathophysiology of otitis media. To elucidate the inflammatory responses that occur during pneumococcal otitis media, the kinetics of the biochemical and cytologic middle ear responses to heat-killed encapsulated and nonencapsulated pneumococci were studied in the chinchilla model. Inoculation of the middle ear cavity with at least 10(6) S pneumoniae cells induced an early, brief vascular response with leakage of small (albumin) followed by larger (alpha 2-macroglobulin) proteins, followed by sustained influx of acute inflammatory cells and lysozyme. The threshold for a sustained lysozyme response was 1,000 times lower for nonencapsulated than for encapsulated pneumococci. These results indicate that nonviable S pneumoniae organisms with an intact envelope initiate the middle ear inflammatory response. Therefore, interventions that enhance the clearance of pneumococcal cells from the middle ear may reduce the inflammatory response and prevent chronic middle ear inflammation.
authors.affiliation: University of Minnesota Otitis Media Research Center, Minneapolis.
authors.forename: N
authors.initials: N
authors.lastname: Nonomura
authors.forename: G S
authors.initials: GS
authors.lastname: Giebink
authors.forename: S K
authors.initials: SK
authors.lastname: Juhn
authors.forename: T
authors.initials: T
authors.lastname: Harada
authors.forename: D
authors.initials: D
authors.lastname: Aeppli
mesh_terms: D000418:Albumins / Q000378:metabolism; D000818:Animals; D002682:Chinchilla; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology; D010033:Otitis Media / Q000209:etiology / Q000503:physiopathology*; D010035:Otitis Media, Suppurative / Q000209:etiology / Q000503:physiopathology; D013290:Streptococcal Infections / Q000503:physiopathology*; D000511:alpha-Macroglobulins / Q000378:metabolism
chemical_list: D000418:Albumins; D000511:alpha-Macroglobulins; D009113:Muramidase