Functional characteristics of sperm obtained by electroejaculation.
Sperm obtained by electroejaculation in 32 anejaculatory men were examined for functional characteristics. Raw specimens showed high sperm counts but motility averaged only 11%. Average viability was 10% for antegrade and 5% for retrograde fractions. Bovine cervical mucus penetration was normal (30 mm. or more in 30 minutes) in only 24% of the electroejaculation samples but it was normal in all of the donor samples tested. Processed sperm motility averaged 30% with 71% forward progression. At 20 hours patient samples retained 46% of the original motility, while donor controls retained 81%. In the hamster egg penetration assay patient sperm penetrated 14% of the oocytes while donor sperm penetrated 40%. Therefore, we identified 4 characteristics of sperm obtained by electroejaculation: 1) low viability, 2) poor survival after overnight incubation, 3) moderately impaired cervical mucus penetration and 4) moderately poor fertilizing capability as measured by the hamster egg penetration assay. Poor sperm survival and impaired function may explain the low pregnancy rates from insemination with electroejaculated sperm.
Denil
J
J
Department of Surgery, University of Michigan Medical Center, Ann Arbor.
Ohl
D A
DA
Menge
A C
AC
Keller
L M
LM
McCabe
M
M
D000328:Adult; D004542:Ejaculation; D004558:Electric Stimulation; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D013076:Sperm Count; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000473:pathology / Q000502:physiology*