Analysis of major histocompatibility complex gene products in tissues isolated from the developing human nervous system.
We have examined the expression of the major histocompatibility complex (MHC) class I and II gene products in the developing human fetal peripheral nervous system. As determined by RNA blot hybridization analysis, MHC class I RNA was readily detectable in extracts prepared from dorsal root ganglia (DRG) obtained from aborted human fetal material. However, utilizing similar methodology, it was not possible to detect MHC class II RNA. In conjunction with these studies, expression of MHC class I and II proteins in primary human fetal DRG tissue was examined by fluorescence-activated flow cytometry and protein immunoblotting. Consistent with the detection of MHC-specific RNA, the accumulation of MHC class I-specific protein was readily detectable in human fetal DRG neural cell populations with little, if any, accumulation of MHC class II-specific protein evident. These studies suggest that MHC gene products may be expressed early in the development of the human nervous system resulting in the generation of specific immunocompetent neural cell populations.
Boyer Kollas
B
B
Department of Microbiology and Immunology, Pennsylvania State University, College of Medicine, Hershey.
Hartle
H T
HT
Wigdahl
B
B
D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000737:chemistry / Q000196:embryology* / Q000276:immunology; D005802:Genes, MHC Class II / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D012313:RNA