Immunological cross-reactivity of the fragments of staphylococcal enterotoxins A and E generated by digestion of proteolytic enzymes.
Three major fragments were generated by limited digestion of staphylococcal enterotoxins A (SEA) and E (SEE) with papain, whereas five major fragments were generated by limited digestion with staphylococcal protease V8 (V8). All of these fragments were detected by immunoblotting with polyclonal anti-SEA and -SEE sera. Some of generated fragments were detected by monoclonal antibodies (MAbs) with specificities for SEA (A-111 and A-211), SEE (E-142), or both (AE-32, AE-37, and AE-53). This indicates that fragments of SEA and SEE containing the type-specific and cross-reacting epitopes may be generated by digestion of the toxins with either papain or V8.
Shinagawa
K
K
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Japan.
Tachihara
R
R
Matsusaka
N
N
Sugii
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D015153:Blotting, Western; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D004768:Enterotoxins / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D008970:Molecular Weight; D010206:Papain / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology*; D012697:Serine Endopeptidases / Q000378:metabolism; D013211:Staphylococcus aureus
D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D000939:Epitopes; D010446:Peptide Fragments; C049463:enterotoxin E, Staphylococcal; C026066:enterotoxin A, Staphylococcal; D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D010206:Papain