title: [The effect of endo-beta-galactosidase on lactosamine sialoantigens Gd, Fl, Vo, Li].
abstract: Human red cells (RBC) were treated with endo-beta-galactosidase from Bacteroides fragilis removing type 2 polylactosamine chains [Galssl-4GlcNAc]n from the RBC surface by cleaving internal Galssl-4Glc(NAc) bonds of linear lactosamine sequences. I and i built up by unsubstituted branched and linear chains were inactivated. The sialo-antigen Vo resembling i antigen expression on adult, newborn and i adult RBC was also inactivated, demonstrating that the Vo determinant is created by sialylation of linear chains. Fl and Gd antigens known to be sialylated polylactosamine chains were not inactivated. Because Gd antigenicity is increased by increasing the length of sugar sequences and Vo determinants are obviously short chain sequences, the action of endo-beta-galactosidase could depend on the length of sialylated sequences on the RBC membrane.
authors.affiliation: Institut für Immunologie, Universität Heidelberg.
authors.forename: D
authors.initials: D
authors.lastname: Roelcke
mesh_terms: D000328:Adult; D000606:Amino Sugars / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects* / Q000276:immunology; D006026:Glycoside Hydrolases; D006801:Humans; D007231:Infant, Newborn; D007519:Isoantigens / Q000097:blood*; D001616:beta-Galactosidase / Q000494:pharmacology*
chemical_list: D000606:Amino Sugars; D007519:Isoantigens; C032233:lactosamine; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase
vernacular_title: Der Effekt von Endo-beta-Galaktosidase auf die Laktosamin-Sialoantigene Gd, Fl, Vo, Li.