Human alpha-fetoprotein and its purification by chromatography on immobilized estrogens.
Human alpha-fetoprotein (AFP) was isolated from human abortive tissue by biospecific chromatography on immobilized estrogens. The most effective sorbents were: estrone-0-3-hemisuccinyl-hexamethylenediamine-Sepharose CL 4B and diethylstilbestrol-diasoanisole-sulfonyl-oxyethyl-Sepharose CL 4B. As elution solution the most optimum was 10% buffered aqueous butanol. Taking into consideration the data obtained, one can conclude that AFP in human biological fluids is bound to immobilized estrogens. Butanol extraction deestrogenizes AFP, and as a result human AFP acquires affinity to immobilized estrogens. During rechromatography on immobilized diethylstilbestrol, it was possible to obtain AFP preparations of about 95% purity. The present results provide the opportunity to work out new methodological approaches to human AFP isolation using biospecific chromatography on immobilized estrogens.
Tatarinov
Y S
YS
Department of Biochemistry and Immunochemical, Second Moscow Medical Institute, USSR.
Terentiev
A A
AA
Moldogazieva
N T
NT
Tagirova
A K
AK
D000418:Albumins / Q000302:isolation & purification; D002845:Chromatography / Q000379:methods; D004054:Diethylstilbestrol / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D005333:Fetus; D006801:Humans; D011247:Pregnancy; D012685:Sepharose; D014020:Tissue Extracts / Q000737:chemistry / Q000302:isolation & purification; D000509:alpha-Fetoproteins / Q000302:isolation & purification* / Q000378:metabolism
D000418:Albumins; D004967:Estrogens; D014020:Tissue Extracts; D000509:alpha-Fetoproteins; D004054:Diethylstilbestrol; D012685:Sepharose