[Determination of the rate of utilization of glucose in a suspension of erythrocytes].
The authors suggest a new method for measuring the rate of glucose utilization in a cell (red cell) suspension, making use of interferometry. This method allows an easy examination of glucose utilization kinetics in time with but 1000 cells. Thermostatic control of the sample in the cuvette of the device permits detecting the temperature effects on glucose metabolism kinetics and thus define the temperature optimal for the process. A correlation was revealed between the point of structure phase transition in the red cell membrane and the optimal temperature for glucose utilization within the range of physiologic temperatures. This method may be useful in medical, biochemical, physiologic, and hematologic studies. The relative error of the technique is 3 percent.
Kunitsyn
V G
VG
Nekrasova
M F
MF
Fedenkov
V I
VI
D001786:Blood Glucose / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007368:Interferometry / Q000379:methods; D007700:Kinetics
D001786:Blood Glucose
Opredelenie skorosti utilizatsii gliukozy vo vzvesi éritrotsitov.