[Isolation and purification of alpha fetal protein utilizing Act-Ultrogel AcA 22 affinity chromatography].
In the present communication, we report a method for the isolation and purification of alpha FP from fetal corpse homogenate in a single-step procedure using Act-Ultrogel AcA 22 affinity chromatogrpahy column. This method proves to be better than the conventional method in simplicity, fastness and validity with recovery, purity and immunoreactivity. The immunoreactivity of the purified alpha FP labelled by the method of Hunter and Greenwood (1962) was 50-60% (B/T%). This was similar to alpha FP standard (WHO). The results indicate that the 125I-alpha FP is suitable for alpha FP radioimmunoassay.
Tang
S
S
Deng
S
S
Zhou
W
W
D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
D000509:alpha-Fetoproteins