title: Translational diffusion and intrinsic viscosity of globular proteins. Theoretical predictions using hydrated hydrodynamic models. Application to BPTI.
abstract: In this paper, we present a way to make hydrodynamic models of globular proteins, including the hydration shell associated with them in aqueous solutions. Theoretical calculations using these models are made in order to determine the hydrodynamic properties of these proteins, employing rigorous and approximate methods of calculation. These will be applied to the bovine pancreatic trypsin inhibitor, BPTI. Several hydrodynamic models are constructed: the A-model for the unhydrated protein BPTI and a set of H-models for hydrated protein with different hydration degrees. Theoretical results for the translational diffusion coefficient Dt and the intrinsic viscosity [eta] are obtained from different models. From the analysis of the A-model and hydrodynamic properties, there is not a clear assignation of an ellipsoidal shape to this protein molecule. An amount of approximately 0.5 g H2O/g protein could be assigned to the BPTI.
authors.affiliation: Departamento de Química Física, Universidad de Extremadura, Badajoz, Spain.
authors.forename: M M
authors.initials: MM
authors.lastname: García
authors.forename: M A
authors.initials: MA
authors.lastname: Jiménez Rios
authors.forename: J M
authors.initials: JM
authors.lastname: García Bernal
mesh_terms: D000818:Animals; D007611:Aprotinin / Q000737:chemistry*; D002417:Cattle; D004058:Diffusion; D008958:Models, Molecular; D014783:Viscosity; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D007611:Aprotinin