title: Antibody BNH9 detects red blood cell-related antigens on anaplastic large cell (CD30+) lymphomas.
abstract: Two new monoclonal antibodies--BNH9 and BNF13--were generated by using a human lung adenocarcinoma cell line and standard hybridoma techniques. Both were found to react with epithelial and endothelial cells in routinely fixed and embedded tissues. Unexpected membrane labelling of some large cell lymphomas while non-reacting with normal lymphoid cells, prompted further characterisation. The antibodies were found to recognise red blood cell-related oligosaccharide antigens. The specificities were directed towards H and Y determinants. A distinctive pattern of reactivity was found for BNH9 in studying 480 cases of various lymphoid neoplasms. Strong expression of H and/or Y antigens was observed in 65/127 (51%) cases of anaplastic large cell(ALC) (CD30+) lymphomas, which are also known to co-express epithelial membrane antigen (EMA) frequently. Only a minority (less than 6%) of other non-Hodgkin's lymphomas (NHL) (CD30-,EMA-; 208 cases) and Hodgkin's disease (HD) (CD30+, EMA-; 126 cases) were positive. Expression of H and Y antigens was inducible on normal lymphocytes by mitogenic stimulation and by Epstein-Barr virus infection. The data suggest remarkable biological differences of ALC lymphomas within NHL and from HD.
authors.affiliation: Department of Anatomical Pathology, CHU Purpan, Toulouse, France.
authors.forename: G
authors.initials: G
authors.lastname: Delsol
authors.forename: A
authors.initials: A
authors.lastname: Blancher
authors.forename: T
authors.initials: T
authors.lastname: al Saati
authors.forename: E
authors.initials: E
authors.lastname: Ralfkiaer
authors.forename: A
authors.initials: A
authors.lastname: Lauritzen
authors.forename: L
authors.initials: L
authors.lastname: Bruigères
authors.forename: P
authors.initials: P
authors.lastname: Brousset
authors.forename: F
authors.initials: F
authors.lastname: Rigal-Huguet
authors.forename: C
authors.initials: C
authors.lastname: Mazerolles
authors.forename: A
authors.initials: A
authors.lastname: Robert
mesh_terms: D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens / Q000276:immunology*; D002472:Cell Transformation, Viral; D000939:Epitopes; D006689:Hodgkin Disease / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000276:immunology*; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D000939:Epitopes