Induction of nitrite production in mouse spleen cells by immunization.
Changes of nitrite production in mouse spleen cells of in vitro secondary antibody response were investigated. Mouse spleen cells immunized with gamma globulin fraction of rat serum produced nitrite 3 days after in vitro challenging with the same antigen. Nitrite production of rabbit IgG-challenged spleen cells was found to be about 2.9-times higher than that of spleen cells primed with the gamma globulin fraction of rat serum. Nitrite production in this system was completely suppressed by T cell depletion (99.7% inhibition). Furthermore, nitrite production in these cells significantly decreased by addition of anti-interferon gamma antibody (62.9% inhibition). These data indicate that nitrite production in antigen-immunized spleen cells is affected with the immunogenicity of an antigen and regulated by T cells, especially interferon (IFN) gamma.
Takagi
K
K
Department of Environment Biochemistry, School of Pharmaceutical Science, University of Shizuoka, Japan.
Nakagami
H
H
Nakano
T
T
Suketa
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002478:Cells, Cultured; D007114:Immunization; D007074:Immunoglobulin G / Q000378:metabolism / Q000494:pharmacology; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008212:Lymphocyte Depletion; D051379:Mice; D009573:Nitrites / Q000378:metabolism*; D013154:Spleen / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D005719:gamma-Globulins / Q000008:administration & dosage* / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009573:Nitrites; D005719:gamma-Globulins; D007371:Interferon-gamma