title: Developmental regulation of CUP gene expression through DNA methylation in Mucor spp.
abstract: Inserts which carried the CUP gene from Saccharomyces cerevisiae or Mucor racemosus were used as hybridization probes to measure the methylation state and expression of the CUP gene from Mucor rouxii at different stages of growth. It was observed that the fungus contains a CUP multigene family. All the CUP genes were present in a hypermethylated DNA region in nongrowing and isodiametrically growing spores and were not transcribed at these stages. After germ tube emergence, CUP genes became demethylated and transcriptionally active. Development, demethylation, and transcription of CUP genes were blocked by the ornithine decarboxylase inhibitor 1,4-diaminobutanone. These results suggest that genes that are activated during development became demethylated in this fungus.
authors.affiliation: Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato, Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Cano-Canchola
authors.forename: L
authors.initials: L
authors.lastname: Sosa
authors.forename: W
authors.initials: W
authors.lastname: Fonzi
authors.forename: P
authors.initials: P
authors.lastname: Sypherd
authors.forename: J
authors.initials: J
authors.lastname: Ruiz-Herrera
mesh_terms: D002352:Carrier Proteins; D004271:DNA, Fungal / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008668:Metallothionein / Q000235:genetics*; D008745:Methylation; D009089:Mucor / Q000235:genetics* / Q000254:growth & development; D005810:Multigene Family; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: C500769:CUP1-1 protein, S cerevisiae; D002352:Carrier Proteins; D004271:DNA, Fungal; C007121:copper thionein; D008668:Metallothionein