cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase.
1. The sensitivity of partially purified low Km phosphodiesterase (PDE) from Mucor rouxii to pharmacological agents and cAMP analogs was studied. The IC50 obtained were compared with those reported for PDEs from higher eukaryotes. 2. The best inhibitors of the hydrolysis of 1 microM cAMP were SQ 65.442 (IC50 c 10 microM), dipyridamol and CI 930. cGMP was not an inhibitor (IC50 greater than 1000 microM). 3. The cAMP analogs were tested as inhibitors of the hydrolysis of 0.1 microM cAMP. 8-Aminohexylamino cAMP was the best inhibitor with an IC50 of c 1 microM. 4. A sedimentation profile of Mucor PDE was assayed in the presence of several pharmacological inhibitors and cAMP analogs. No isoforms with different sensitivity towards the inhibitors were detected. Forms with slightly different behaviour towards some cAMP analogs were observed.
Tomes
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Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Republica Argentina.
Moreno
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D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D004176:Dipyridamole / Q000494:pharmacology; D006868:Hydrolysis; D009089:Mucor / Q000201:enzymology*; D009539:Nicotinic Acids / Q000494:pharmacology; D010208:Papaverine / Q000494:pharmacology; D011724:Pyridazines / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology
D009539:Nicotinic Acids; D011724:Pyridazines; C067297:8-aminohexylamino cAMP; C027166:SQ 65442; D004176:Dipyridamole; C042528:4,5-dihydro-6-(4-(imidazol-1-yl)phenyl)-5-methyl-3(2H)-pyridazinone; D013806:Theophylline; D010208:Papaverine; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine