title: Production of MUC1 and MUC2 mucins by human tumor cell lines.
abstract: A mucus secreting, clonal derivative (HT29-SB) of the human colonic adenocarcinoma cell line HT29, and the LS174T colon cancer cell line, secrete mucin into the culture medium as a viscoelastic gel. Mab BC2, which defines a peptide epitope present in the variable number of tandem repeats (VNTR) of the MUC1 core protein, reacted with this material after deglycosylation. Two high molecular weight bands were detected in TFMSA treated gel-formed mucin from HT29-SB and LS174T by western blotting (Mr 580 kDa and 420 kDa). A similar pattern of reactivity was seen with the culture supernatants from HT29-SB, the ovarian tumor cell line COLO-316, and the breast cancer cell line MCF-7. Mab CCP58 (anti-MUC2 VNTR) reacted with a 580 kDa band in gel-formed mucin produced by LS174T, but was not reactive with mucin produced by the other cell lines. The findings indicate that human colonic cell lines, in addition to breast and ovarian cell lines, may both express and secrete the MUC1 protein core, and that the LS174T cell line expresses and secretes both the MUC1 and MUC2 core proteins.
authors.affiliation: Medical Innovations Limited, Labrador, Australia.
authors.forename: P L
authors.initials: PL
authors.lastname: Devine
authors.forename: G T
authors.initials: GT
authors.lastname: Layton
authors.forename: B A
authors.initials: BA
authors.lastname: Clark
authors.forename: G W
authors.initials: GW
authors.lastname: Birrell
authors.forename: B G
authors.initials: BG
authors.lastname: Ward
authors.forename: P X
authors.initials: PX
authors.lastname: Xing
authors.forename: I F
authors.initials: IF
authors.lastname: McKenzie
mesh_terms: D000230:Adenocarcinoma; D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line; D003110:Colonic Neoplasms; D000939:Epitopes / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009077:Mucins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D009093:Mucus / Q000737:chemistry; D011485:Protein Binding; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D000951:Antigens, Neoplasm; D000939:Epitopes; D009077:Mucins