B cells of chronic lymphatic leukemia express V genes in unmutated form.
In order to investigate whether the leukemic B cells in B-CLL express immunoglobulin genes in mutated or unmutated form, independent cDNA clones of one patient with B-CLL expressing heavy and light chain V region genes were sequenced. The sequences of both the VH and V kappa clones were identical. The V genes could be assigned to known germline V genes. No somatic mutations were found. At the V kappa-J kappa border there is an insertion of N-sequences which are only rarely found in immunoglobulin light chain genes. Our study confirms other published data on V gene expression in B-CLL in that the surface immunoglobulins in these tumors are unmutated.
Küppers
R
R
I. Medizinische Universitätsklinik Köln, F.R.G.
Gause
A
A
Rajewsky
K
K
D000943:Antigens, Differentiation / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology; D001483:Base Sequence; D015139:Blotting, Southern; D018956:CD5 Antigens; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007075:Immunoglobulin M / Q000235:genetics; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data
D000943:Antigens, Differentiation; D000954:Antigens, Surface; D018956:CD5 Antigens; D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M