Generation and characterization of two monoclonal self-associating IgG rheumatoid factors from a rheumatoid synovium.
OBJECTIVE
To study IgG rheumatoid factor (RF) from rheumatoid synovium.


METHODS
We fused the K6H6/B5 human-mouse heterohybridoma with unstimulated rheumatoid synovial B cells to generate IgG-RF-secreting hybridomas.


RESULTS
The RFs from 2 such hybridomas bound specifically to the Fc fragment of human IgG and self-associated to form immune complexes. Such immune complexes are a major characteristic of the pathogenic IgG-RFs in rheumatoid synovium.


CONCLUSIONS
IgG-RF-secreting hybridomas have been obtained. Analyses may reveal the underlying mechanisms of the induction of IgG-RF.
Lu
E W
EW
Department of Medicine, University of California, San Diego 92093.
Deftos
M
M
Tighe
H
H
Carson
D A
DA
Chen
P P
PP
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000473:pathology; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D012217:Rheumatoid Factor / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D013583:Synovial Membrane / Q000737:chemistry* / Q000276:immunology
D007074:Immunoglobulin G; D012217:Rheumatoid Factor