Cloning of the human serotonin 5-HT2 and 5-HT1C receptor subtypes.
We report the cloning and the deduced amino acid sequence of cDNAs encoding both the human serotonin 5-HT2 and 5-HT1C receptors. The human 5-HT2 and 5-HT1C receptors shared 87% and 90% amino acid homology, respectively, with their rat counterparts. The most divergent regions of the 5-HT2 receptor between human and rat were the N-terminal extracellular domain (75% homology) and the C-terminal intracellular domain (67% homology between amino acids 426-474). The greatest variability between the human and rat 5-HT1C receptors were at the N-terminal extracellular domain (78% homology) and the third cytoplasmic loop (71% homology). The availability of the cloned human 5-HT2 and 5-HT1C receptors will help facilitate the further understanding of the molecular pharmacology and physiology of these receptors.
Saltzman
A G
AG
Rhône-Poulenc Rorer Central Research, King of Prussia, PA 19406.
Morse
B
B
Whitman
M M
MM
Ivanshchenko
Y
Y
Jaye
M
M
Felder
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001933:Brain Stem / Q000502:physiology*; D002421:Caudate Nucleus / Q000502:physiology*; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D051381:Rats; D011985:Receptors, Serotonin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D011061:Poly A; D012313:RNA; D004247:DNA