Transcription of sequences upstream of the rat prolactin gene suggests the existence of a second promoter.
The transcription of the rat prolactin gene domain has been examined using a modified Southern blot procedure. Cloned genomic DNAs were resolved by electrophoresis in agarose, transferred to nitrocellulose, and probed with radiolabeled RNA that had been synthesized in vitro by nuclei isolated from pituitary tumor cells. Data presented in this paper illustrate that single copy genomic sequences located within 7.3 kb upstream of exon 1 are transcribed. Single copy or low copy number DNA sequences that reside greater than 7.3 kb upstream of exon 1, or downstream of exon 5 were not transcribed at detectable levels. These data suggest that a second promoter may exist upstream of the rat prolactin gene and that this second promoter may be active in pituitary cells.
Shull
J D
JD
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-6805.
D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism; D010911:Pituitary Neoplasms / Q000139:chemically induced / Q000378:metabolism; D011388:Prolactin / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D011916:Rats, Inbred F344; D014158:Transcription, Genetic
D012313:RNA; D011388:Prolactin