A toxic substance from the sea urchin Toxopneustes pileolus induces histamine release from rat peritoneal mast cells.
A toxic substance (P-II fraction), fractionated from the pedicellariae of the sea urchin Toxopneustes pileolus, dose-dependently caused the histamine release from rat peritoneal mast cells. The histamine release induced by P-II fraction increased with time, while compound 48/80 caused a more rapid histamine release. The dose-response curve for P-II fraction was studied with concentration 0.03-2.0 mg/ml. This reaction was dependent on Ca2+ and temperature. When glucose (5.5 mM) was omitted during the incubation step, the histamine release induced by P-II fraction was significantly reduced as compared to that of compound 48/80. Pyruvate reversed this reduction. On the other hand, the histamine release induced by P-II fraction was effectively potentiated by the addition of glucose (11.0 mM), but not that by compound 48/80. These results suggest that P-II fraction-induced histamine release differs from that of compound 48/80 disregards to the effects of glucose, because this histamine release appears to be more sensitive to the glycolytic pathway than compound 48/80-induced histamine release.
Takei
M
M
Department of Pharmacology, School of Pharmacy, Tokushima Bunri University, Japan.
Nakagawa
H
H
Kimura
A
A
Endo
K
K
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003847:Deoxyglucose / Q000494:pharmacology; D006636:Histamine Release; D007700:Kinetics; D008387:Marine Toxins / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology*; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid; D012617:Sea Urchins / Q000032:analysis*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D008387:Marine Toxins; D011773:Pyruvates; D003189:p-Methoxy-N-methylphenethylamine; D019289:Pyruvic Acid; D003847:Deoxyglucose; D002118:Calcium