Monoaminergic regulation of proopiomelanocortin messenger RNA concentrations in primary cell cultures of rat hypothalamus.
The effect of monoaminergic neurotransmitters on a 1.1-kb proopiomelanocortin messenger RNA (POMC mRNA) detected in rat hypothalamic cells maintained in culture has been evaluated. Serotonin caused a 15% increase in POMC mRNA levels, an effect which was blocked by the 5-HT2 receptor antagonist ketanserin. Dopamine markedly decreased POMC mRNA levels in a dose related manner. Haloperidol and the selective D2 antagonist (+)-butaclamol prevented the inhibitory effects of both dopamine and the selective D2 agonist, 2-bromo-alpha-ergocryptine. The selective dopamine D1 receptor agonist, SKF 38393, as well as norepinephrine and acetylcholine did not affect POMC mRNA levels. It is concluded that serotonin exerts a positive control and dopamine a negative control on POMC mRNA concentrations in primary cultures of rat hypothalamic neurons. The negative effect of dopamine appears to be exerted via D2 receptor-mediated mechanism.
L'Héreault
S
S
Molecular Psychogenetics Laboratory, CHUL Research Centre, Ste Foy, Que. Canada.
Barden
N
N
D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology*; D002069:Butaclamol / Q000494:pharmacology; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology*; D004622:Embryo, Mammalian; D006220:Haloperidol / Q000494:pharmacology; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D009638:Norepinephrine / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D011333:Pro-Opiomelanocortin / Q000235:genetics*; D015347:RNA Probes; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D015347:RNA Probes; D012333:RNA, Messenger; D001971:Bromocriptine; D011333:Pro-Opiomelanocortin; D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine; D003907:Dexamethasone; D002069:Butaclamol; D006220:Haloperidol; D000109:Acetylcholine; D004298:Dopamine; D009638:Norepinephrine