[The detection of the Marburg virus antigen by solid-phase immunoenzyme analysis].
Comparative studies of two variants of the enzyme-linked immunosorbent assay (ELISA) were carried out to determine the sensitivity of the detection of Marburg virus antigens in Vero cells. Both competitive and two-antibody ELISA variants detected as little as 5 ng of Marburg virus antigen. The Vero cell monolayer was found to produce 5-50 ng/0.05 ml of the virus-specific proteins at 6 to 8 days postinfection.
Vladyko
A S
AS
Chepurnov
A A
AA
Bystrova
S I
SI
Lemeshko
N N
NN
Lukashevich
I S
IS
D000818:Animals; D000956:Antigens, Viral / Q000097:blood* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D000939:Epitopes / Q000097:blood / Q000302:isolation & purification; D006168:Guinea Pigs; D007114:Immunization; D029024:Marburgvirus / Q000276:immunology*; D011817:Rabbits; D012680:Sensitivity and Specificity; D012692:Serial Passage; D013997:Time Factors; D014709:Vero Cells
D000956:Antigens, Viral; D000939:Epitopes
Vyiavlenie antigena virusa Marburg metodom tverdofaznogo immunofermentnogo analiza.