Combined effect of acute denervation and ischemia on the microcirculation of skeletal muscle.
Using direct in vivo videomicroscopy and a fluorescein dye technique, reperfusion injury after 3 h of ischemia was studied in the acutely denervated cremaster muscle of the rat. Compared with normally innervated controls, ischemia-induced reperfusion injury was more severe in the denervated group and included a delay of blood flow recovery, vortex formation, edema, hemorrhage, and vessel spasm. Vessel size was reduced at the arteriole and small artery level, and there was a decrease of reactive hyperemia. The injury mechanism may be related to a loss of active vasomotion and vascular response to vasoactive substances after denervation. The results suggest that shortening the ischemia time of denervated tissues may reduce ischemia-induced reperfusion injury. Similarly, given the same ischemia time, improved tissue reperfusion may be expected if the nerve supply is maintained.
Chen
L E
LE
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710.
Seaber
A V
AV
Urbaniak
J R
JR
D000818:Animals; D003714:Denervation; D019793:Fluorescein; D005452:Fluoresceins; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D008833:Microcirculation; D009132:Muscles / Q000098:blood supply* / Q000294:innervation; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D015424:Reperfusion; D014661:Vasoconstriction
D005452:Fluoresceins; D019793:Fluorescein