Molecular and functional analysis of the virus- and interferon-inducible human MxA promoter.
The virus- and interferon-inducible human MxA (IFI-78k) gene is a homologue of the murine influenza resistance gene Mx1. Three overlapping human cosmid clones covering most of the gene including its promoter region were isolated. Sequencing the 5' MxA cDNA derived by RT-PCR (reverse transcriptase-polymerase chain reaction) confirmed the most 5' putative transcriptional start site. The MxA promoter does not contain a TATA or CCAAT box but has three Interferon Stimulated Response Element (ISRE) motifs. Strong induction with type I interferons was demonstrated with a fragment containing only two ISREs in human L132 cells. This induced expression was not adversely affected by 2-aminopurine. However, the promoter showed constitutive expression in transiently or stably transfected murine LM cells.
Chang
K C
KC
Unit of Veterinary Molecular and Cellular Biology, Royal Veterinary College, University of London, U.K.
Hansen
E
E
Foroni
L
L
Lida
J
J
Goldspink
G
G
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA; D019204:GTP-Binding Proteins; D015870:Gene Expression; D015698:Genomic Library; D006801:Humans; D007370:Interferon Type I / Q000502:physiology; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D064547:Myxovirus Resistance Proteins; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection
D007370:Interferon Type I; C579071:MX1 protein, human; C579070:Mx1 protein, mouse; D064547:Myxovirus Resistance Proteins; D011506:Proteins; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D012194:RNA-Directed DNA Polymerase; D019204:GTP-Binding Proteins