title: Ion channels in cultured adult human Schwann cells.
abstract: Single channel currents have been recorded from cultured adult human Schwann cells. In both cell-attached and -excised (inside-out) patches, openings from a high-conductance (360 pS) channel were observed; measurements of the zero-current potential indicated that the channel was predominantly selective for chloride. Depolarizing and hyperpolarizing voltage steps activated the anion channel, which subsequently reverted to a closed state even in the presence of the maintained step. A second channel, with a conductance near 20 pS and with a current amplitude that increased with patch hyperpolarization, passed inward K+ currents in both cell-attached and inside-out patches. The mean open times for this channel were near 20 ms at the cell resting potential and decreased with patch hyperpolarization. The presence of these anion and cation selective channels in the human Schwann cell membrane would be consistent with a role for the cells in the regulation of extracellular K+.
authors.affiliation: Department of Pharmacology and Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
authors.forename: J G
authors.initials: JG
authors.lastname: McLarnon
authors.forename: S U
authors.initials: SU
authors.lastname: Kim
mesh_terms: D000838:Anions / Q000378:metabolism; D002412:Cations / Q000378:metabolism; D002478:Cells, Cultured; D004594:Electrophysiology; D006801:Humans; D007473:Ion Channels / Q000378:metabolism* / Q000502:physiology; D015221:Potassium Channels / Q000378:metabolism / Q000502:physiology; D012583:Schwann Cells / Q000378:metabolism*
chemical_list: D000838:Anions; D002412:Cations; D007473:Ion Channels; D015221:Potassium Channels