Polyadenylated RNA sequences produced in vaccinia virus-infected cells under aberrant conditions inhibit protein synthesis in vitro.
We have previously demonstrated that small nontranslated polyadenylated RNAs (POLADS) produced in vaccinia virus (VV)-infected cells inhibit the translation of cellular mRNAs, but minimally affect the translation of VV mRNAs in a cell-free protein synthesizing system. Infection of HeLa cells with ultraviolet-irradiated vaccinia virus or infection in the presence of actinomycin D (ACD) amplifies the synthesis of POLADS compared to the amount produced in cells infected under normal conditions. The effect of these POLADS on translation was studied in the reticulocyte lysate system. Polyadenylated RNAs isolated from cells infected with wild-type virus (V-POLADS) had a greater inhibitory effect on HeLa cell protein synthesis than on VV protein synthesis. Polyadenylated sequences obtained from cells infected with ultraviolet-irradiated virus (UV-POLADS) or from cells infected in the presence of ACD (ACD-POLADS), however, inhibited translation of both HeLa and viral mRNAs. Ultraviolet-POLADS and ACD-POLADS were found to possess, on average, longer poly(A) tails than V-POLADS. The inhibition of translation of both host and viral mRNAs effected by V-POLADS, UV-POLADS, and ACD-POLADS was reversed by poly(A) binding protein.
Cacoullos
N
N
Department of Microbiology and Immunology, State University of New York, Brooklyn 11203.
Bablanian
R
R
D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D011061:Poly A / Q000737:chemistry / Q000235:genetics*; D014176:Protein Biosynthesis; D012313:RNA / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D014615:Vaccinia / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics*
D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D012313:RNA