Diacylglycerol accumulation is involved in the potentiating effect of A23187 on carbachol-stimulated amylase secretion from parotid gland.
We studied the role of sn-1, 2-diacylglycerol (DAG) accumulation in the potentiating effect of A23187 on carbachol (CCh)-stimulated amylase secretion from rat parotid acinar cells. A23187 (0.1 microM) linearly increased DAG accumulation with time for at least 30 min. At concentrations higher or lower than 0.1 microM, DAG levels increased for up to 20 min, but declined at 30 min. Dose-response curve for DAG accumulation induced by A23187 was similar to that for amylase secretion. A23187 augmented CCh-stimulated DAG accumulation and amylase secretion. These results suggest that DAG accumulation is involved in the potentiating effect of A23187 on CCh-stimulated amylase secretion.
Komabayashi
T
T
Department of Physiology and Pharmacology, Tokyo College of Pharmacy, Japan.
Yakata
A
A
Izawa
T
T
Suda
K
K
Tsuboi
M
M
D000681:Amylases / Q000096:biosynthesis* / Q000378:metabolism; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002217:Carbachol / Q000494:pharmacology; D004075:Diglycerides / Q000096:biosynthesis* / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004790:Enzyme Induction / Q000187:drug effects; D008297:Male; D010306:Parotid Gland / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
C011439:1,2-diacylglycerol; D004075:Diglycerides; D000001:Calcimycin; D002217:Carbachol; D000681:Amylases