Oligoclonal expansion and CD1 recognition by human intestinal intraepithelial lymphocytes.
A human intestinal intraepithelial lymphocyte (IEL) T cell line was established from jejunum to characterize the structure and function of the alpha beta T cell antigen receptors (TCRs) expressed by this population. Single-sided polymerase chain reaction (PCR) amplification cloning and quantitative PCR amplification of the TCR chains from the cell line and from fresh IELs demonstrated that IELs were oligoclonal. The IEL T cell line exhibited CD1-specific cytotoxicity and a dominant IEL T cell clone was CD1c-specific. Thus, human jejunal intraepithelial lymphocytes are oligoclonal and recognize members of the CD1 gene family.
Balk
S P
SP
Hematology-Oncology Division, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215.
Ebert
E C
EC
Blumenthal
R L
RL
McDermott
F V
FV
Wucherpfennig
K W
KW
Landau
S B
SB
Blumberg
R S
RS
D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D018949:Antigens, CD1; D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells; D004848:Epithelium / Q000502:physiology; D006801:Humans; D007583:Jejunum / Q000276:immunology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; D018949:Antigens, CD1; D015345:Oligonucleotide Probes; D011948:Receptors, Antigen, T-Cell