title: Identification of a cationic channel in synaptosomal membranes.
abstract: Synaptosomal membranes were fused with liposomes using the 'hydration technique' to produce giant proteoliposomes amenable to patch clamp recordings. Single channel currents of a cationic channel with particular properties were detected. In a solution of 150 mM NaCl, the channel displayed a unit conductance of 136 pS and a mean open state lifetime of 1.1 ms. The gating of the channel was shown to be voltage as well as calcium dependent. Pharmacological studies revealed that the channel was insensitive to a variety of channel blockers, but was inactivated by ruthenium red. Presumably, this channel may play a role in regulating the evoked release of neurotransmitters.
authors.affiliation: Institut für Zoophysiologie, Universität Stuttgart-Hohenheim, Federal Republic of Germany.
authors.forename: E
authors.initials: E
authors.lastname: Tareilus
authors.forename: W
authors.initials: W
authors.lastname: Hanke
authors.forename: H
authors.initials: H
authors.lastname: Breer
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology; D002412:Cations / Q000378:metabolism; D006110:Grasshoppers; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry; D011510:Proteolipids / Q000378:metabolism; D012430:Ruthenium Red / Q000494:pharmacology; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002412:Cations; D007473:Ion Channels; D008081:Liposomes; D011510:Proteolipids; C020485:proteoliposomes; D012430:Ruthenium Red; D002118:Calcium