Interleukin-1-induced cartilage degradation is independent of substance P level in rabbit knees.
The effect of SP on recombinant human IL-1 alpha-induced cartilage degradation in rabbit knees was investigated. Co-injection of 10 ng IL-1 and 1 micrograms SP did not increase cartilage degradation above the level observed after injection of 10 ng IL-1 alone. Co-injection of 100 ng IL-1 and 150 micrograms Spantide, a potent SP antagonist, did not reduce the level of cartilage degradation. Infusion of 100 ng SP/day for two weeks did not induce cartilage degradation. These observations suggest that cartilage degradation induced by IL-1 is independent of SP concentrations in rabbit knees.
O'Byrne
E M
EM
CIBA-GEIGY Corporation, Research Department, Summit, NJ 07901.
Goldberg
R L
RL
Doughty
J R
JR
Wong
M
M
Blancuzzi
V
V
Wilson
D
D
Jeng
A Y
AY
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000473:pathology; D006614:Hindlimb; D007270:Injections, Intra-Articular; D007375:Interleukin-1; D007592:Joint Diseases / Q000139:chemically induced / Q000378:metabolism*; D007596:Joints / Q000378:metabolism; D007632:Keratan Sulfate / Q000378:metabolism; D011817:Rabbits; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000037:antagonists & inhibitors; D011994:Recombinant Proteins; D013373:Substance P / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology
D007375:Interleukin-1; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D011994:Recombinant Proteins; D013373:Substance P; D007632:Keratan Sulfate; C042867:spantide