[Localization of the site of human transferrin interacting with cellular receptors using monoclonal antibodies].
Antigenic determinants of the human transferrin molecule on the sublobe and lobe levels were localized for 7 monoclonal antibodies. Antibodies used have different effects on the interaction of the transferrin with its receptor. It was concluded that transferrin-receptor recognition was determined by NH2-lobe, the N2-sublobe playing major part. Dimerization of the transferrin molecules in solution was detected. Using the panel of monoclonal antibodies it was shown that dimerization accomplished by means of the COOH-lobes of transferrin molecules, the sites of interaction of the NH2-lobe with receptor being exposed. A model of the transferrin - receptor complex is proposed.
Rubikaĭte
B I
BI
Beresten'
S F
SF
Viklitskiĭ
V
V
Slavichkova
A
A
D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006801:Humans; D006868:Hydrolysis; D011863:Radioimmunoassay; D011990:Receptors, Transferrin / Q000378:metabolism*; D014168:Transferrin / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D004220:Disulfides; D000939:Epitopes; D011990:Receptors, Transferrin; D014168:Transferrin
Lokalizatsiia uchastka vzaimodeĭstviia transferrina cheloveka s kletochnym retseptorom s pomoshchĭu monoklonal'nykh antitel.