Differential effects of all-trans and 13-cis-retinoic acid on mRNA levels of nuclear retinoic acid receptors in rat lung and liver.
The effects of three retinoids, all-trans-retinoic acid (all-trans-RA), 13-cis-RA, and etretin were examined on mRNA abundance of nuclear retinoic acid receptors (RAR-alpha, beta, and gamma) in lung and liver of retinol deficient and chow fed rats. All-trans-RA increased lung RAR-beta mRNA levels 5 or 11-fold in chow fed and retinol deficient rats, respectively. Similarly to lung, liver RAR-beta mRNA levels were 3-fold higher in retinol deficient rats fed all-trans-RA than the rats fed cottonseed oil. Lung RAR-gamma mRNA levels were also induced 2-fold by all-trans-RA. In contrast to this, 13-cis-RA and etretin at equimolar doses failed to enhance lung or liver RAR-beta or lung RAR-gamma mRNA levels in retinol deficient rats. These data for the first time show that all-trans-RA is more effective than its 13-cis-isomer in regulating the expression of RAR-beta and gamma transcripts in adult animal.
Haq
R
R
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Pfahl
M
M
Chytil
F
F
D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D018168:Receptors, Retinoic Acid; D012016:Reference Values; D013237:Stereoisomerism; D014212:Tretinoin / Q000494:pharmacology*
D002352:Carrier Proteins; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D014212:Tretinoin; D012313:RNA