title: Correlation of 125I-LSD autoradiographic labeling with serotonin voltage clamp responses in Aplysia neurons.
abstract: Autoradiographic receptor binding studies using 125I-LSD (2-[125I]lysergic acid diethyamide) revealed intense labelling on the soma of a symmetrically located pair of cells in the abdominal ganglion of Aplysia californica. This binding was blocked by micromolar concentrations of serotonin and lower concentrations of the serotonergic antagonists, cyproheptadine and mianserin (Kadan and Hartig, 1988). Electrophysiological investigation of responses to serotonin of neurons in the left upper quadrant, where one of the labeled neurons is located, revealed a range of serotonin responses. Cells L3 and L6 have a K+ conductance increase in response to serotonin that is not blocked by cyproheptadine or mianserin. Cells L2 and L4 have a biphasic response to serotonin: a Na+ conductance increase, which can be blocked by cyproheptadine and mianserin, followed by a voltage dependent Ca2+ conductance which is blocked by Co2+ but not the serotonergic antagonists. Cell L1, and its symmetrical pair, R1, have in addition to the Na+ and Ca2+ responses observed in L2 and L4, a Cl- conductance increase blocked by LSD, cyproheptadine and mianserin. LSD had little effect on the other responses. We conclude that the symmetrically located cells L1 and R1 have a Cl- channel linked to a cyproheptadine- and mianserin-sensitive serotonin receptor that is selectively labelled by 125I-LSD. This receptor has many properties in common with the mammalian serotonin 1C receptor.
authors.affiliation: Wadsworth Center for Laboratories and Research, New York State Department of Health, State University of New York, Albany 12237.
authors.forename: M L
authors.initials: ML
authors.lastname: Evans
authors.forename: M J
authors.initials: MJ
authors.lastname: Kadan
authors.forename: P R
authors.initials: PR
authors.lastname: Hartig
authors.forename: D O
authors.initials: DO
authors.lastname: Carpenter
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001048:Aplysia; D003533:Cyproheptadine / Q000494:pharmacology; D004553:Electric Conductivity / Q000187:drug effects; D005724:Ganglia / Q000502:physiology; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology; D008238:Lysergic Acid Diethylamide / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D008803:Mianserin / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D011869:Radioligand Assay; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology*
chemical_list: D007457:Iodine Radioisotopes; D007473:Ion Channels; C031182:LSD receptor; D011985:Receptors, Serotonin; D008803:Mianserin; D003533:Cyproheptadine; D012701:Serotonin; D008238:Lysergic Acid Diethylamide; D000109:Acetylcholine