title: Promyelocytic leukemia cell line, HL-60, produces human hepatocyte growth factor.
abstract: The human promyelocytic leukemia cell line, HL-60, stimulated with PMA, produced human HGF-like immunoreactivity (HL-60 HGF), which was detected with human HGF-specific ELISA. The purified HL-60 HGF was indistinguishable from human HGF in the plasma of patients with fulminant hepatic failure by studies of subunit constitution and amino acid composition. The HL-60 HGF mRNA corresponded to 6 kb, which was consistent with previous reported data in rat and human HGF mRNA, was detected in stimulated HL-60, by northern hybridization analysis using human HGF cDNA probe. These findings indicated that HL-60 HGF was identical to, or closely resembled, human plasma HGF. The HL-60 cell is an attractive model for studies of HGF-producing mechanisms, the manner of secretion and the nature of induction signals.
authors.affiliation: Cellular Technology Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishino
authors.forename: N
authors.initials: N
authors.lastname: Kaise
authors.forename: Y
authors.initials: Y
authors.lastname: Sindo
authors.forename: N
authors.initials: N
authors.lastname: Nishino
authors.forename: T
authors.initials: T
authors.lastname: Nishida
authors.forename: S
authors.initials: S
authors.lastname: Yasuda
authors.forename: Y
authors.initials: Y
authors.lastname: Masui
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004261:DNA Replication / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006133:Growth Substances / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D017228:Hepatocyte Growth Factor; D006801:Humans; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute; D008099:Liver / Q000187:drug effects / Q000502:physiology; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D003470:Culture Media; D006133:Growth Substances; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D017228:Hepatocyte Growth Factor; D013755:Tetradecanoylphorbol Acetate