Monoclonal antibodies raised against post-translational domains of the electroplax sodium channel.
Eleven monoclonal antibodies were identified that recognized eel electroplax sodium channels. All the monoclonal antibodies specifically immunostained the mature TTX-sensitive sodium channel (Mr 265,000) on immunoblots. None of the monoclonal antibodies would precipitate the in vitro translated channel core polypeptide in solution. One monoclonal antibody, 3G4, was found to bind to an epitope involving terminal polysialic acids. Extensive digestion of the channel by the exosialidase, neuraminidase, or partial polysialic acid removal by the endosialidase, endo-N-acetylneuraminidase, destroy the 3G4 epitope. 3G4 is, therefore, a highly selective probe for the post-translationally attached polysialic acids. Except for this monoclonal antibody, the epitopes recognized by the remaining antibodies were highly resistant to extensive N-linked deglycosylation. Thus, the monoclonal antibodies may be directed against unique post-translationally produced domains of the electroplax sodium channel, presumably sugar groups that are abundant on this protein (Miller, J.A., Agnew, W.S., Levinson, S.R. 1983, Biochemistry 22:462-470). These monoclonal antibodies should prove useful as tools to study discrete post-translational processing events in sodium channel biosynthesis.
Ivey
S
S
Department of Physiology, University of Colorado Medical School, Denver 80262.
Thornhill
W B
WB
Levinson
S R
SR
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004593:Electrophorus / Q000378:metabolism*; D000939:Epitopes; D006026:Glycoside Hydrolases; D011499:Protein Processing, Post-Translational; D012794:Sialic Acids / Q000276:immunology; D015222:Sodium Channels / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000939:Epitopes; D012794:Sialic Acids; D015222:Sodium Channels; C021319:polysialic acid; D006026:Glycoside Hydrolases