Detection of Helicobacter pylori in stomach tissue by use of a monoclonal antibody.
Monoclonal antibodies were produced against an acid glycine extract of Helicobacter pylori ATCC 43504T. One of these appeared to be specific for H. pylori; it recognized all H. pylori isolates by an indirect immunofluorescence assay (IIF) but it did not cross-react with the other strains tested, including different species of the genera Helicobacter, Campylobacter, and Wolinella. Different strains of members of the families Enterobacteriaceae and Pseudomonadaceae or other gram-negative bacteria tested also gave negative reactions. Indirect immunofluorescence assay of antral biopsy specimens identified 54 of 56 infected patients (96.4%), and it may be able to detect nonviable organisms after antibiotic therapy.
Husson
M O
MO
Laboratoire de Bactériologie A, Faculté de Médecine, Lille, France.
Leclerc
H
H
D000166:Acridines; D000818:Animals; D000911:Antibodies, Monoclonal; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011706:Pyloric Antrum / Q000382:microbiology*; D013194:Staining and Labeling; D014510:Urease
D000166:Acridines; D000911:Antibodies, Monoclonal; D014510:Urease