title: Characterization of a neu/c-erbB-2 protein-specific activating factor.
abstract: The neu oncogene encodes a tyrosine kinase with growth factor receptor-like properties. A neu protein-specific activating factor (NAF) was partially purified from medium conditioned by the transformed human T-cell line ATL-2. NAF was able to stimulate the tyrosine-specific kinase activity of the neu protein (p185neu), induce dimerization and internalization, and increase the growth of cells bearing the neu protein. The effects of NAF were mediated by an interaction with the p185neu extracellular domain. NAF had no effect on the epidermal growth factor receptor kinase activity and no effect on cells that express that receptor. Further analysis of NAF and of other recently described neu protein-activating activities should help clarify the role of the neu protein in cell growth and transformation.
authors.affiliation: Division of Immunology, University of Pennsylvania School of Medicine, Philadelphia.
authors.forename: K
authors.initials: K
authors.lastname: Dobashi
authors.forename: J G
authors.initials: JG
authors.lastname: Davis
authors.forename: Y
authors.initials: Y
authors.lastname: Mikami
authors.forename: J K
authors.initials: JK
authors.lastname: Freeman
authors.forename: J
authors.initials: J
authors.lastname: Hamuro
authors.forename: M I
authors.initials: MI
authors.lastname: Greene
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D015536:Down-Regulation; D004789:Enzyme Activation; D006801:Humans; D008024:Ligands; D046911:Macromolecular Substances; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D011940:Receptor Aggregation; D018719:Erb-b2 Receptor Tyrosine Kinases; D013601:T-Lymphocytes / Q000737:chemistry; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D008024:Ligands; D046911:Macromolecular Substances; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D014443:Tyrosine