Immobilized metal ion affinity partitioning, a method combining metal-protein interaction and partitioning of proteins in aqueous two-phase systems.
Immobilized metal ions were used for the affinity extraction of proteins in aqueous two-phase systems composed of polyethylene glycol (PEG) and dextran or PEG and salt. Soluble chelating polymers were prepared by covalent attachment of metal-chelating groups to PEG. The effect on the partitioning of proteins of such chelating PEG derivatives coordinated with different metal ions is demonstrated. The proteins studied were alpha 2-macroglobulin, tissue plasminogen activator, superoxide dismutase and monoclonal antibodies. The results indicate that immobilized metal ion affinity partitioning provides excellent potential for the extraction of proteins.
Birkenmeier
G
G
Institute of Biochemistry, Karl-Marx-Universität Leipzig, Germany.
Vijayalakshmi
M A
MA
Stigbrand
T
T
Kopperschläger
G
G
D000911:Antibodies, Monoclonal / Q000032:analysis / Q000378:metabolism; D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods*; D003911:Dextrans; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000378:metabolism*; D011092:Polyethylene Glycols; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000032:analysis / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000032:analysis / Q000378:metabolism
D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D003911:Dextrans; D008670:Metals; D011506:Proteins; D000511:alpha-Macroglobulins; D011092:Polyethylene Glycols; D013482:Superoxide Dismutase; D010959:Tissue Plasminogen Activator