Sequence of a cDNA clone encoding pig heart mitochondrial CoA transferase.
We have isolated a full-length cDNA clone encoding the cytoplasmic precursor to pig heart mitochondrial CoA transferase (succinyl-CoA:3-ketoacid coenzyme A transferase (3-oxoacid CoA transferase, EC 2.8.3.5], a key enzyme for ketone body catabolism. The deduced amino acid sequence indicates the presence of a 39-residue mitochondrial signal sequence and a 481-residue mature protein of molecular weight 52,197. CoA transferase is known to be susceptible to proteolytic cleavage to produce a nicked but active enzyme. We have identified the site of proteolysis, and analysis of the sequence in its vicinity suggests that the polypeptide may fold into two domains connected by a highly hydrophilic bridge.
Lin
T W
TW
Department of Biochemistry, University of Alberta, Edmonton, Canada.
Bridger
W A
WA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015256:Coenzyme A-Transferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D006868:Hydrolysis; D008929:Mitochondria, Heart / Q000201:enzymology*; D008969:Molecular Sequence Data; D013552:Swine; D014162:Transfection
D004247:DNA; D015256:Coenzyme A-Transferases