Clastogenic effects of bleomycin, cyclophosphamide, and ethyl methanesulfonate on resting and proliferating human B- and T-lymphocytes.
The effects of bleomycin (BM), cyclophosphamide (CP), and ethyl methanesulfonate (EMS) on the frequencies of chromosomal aberrations were tested in mitogen-stimulated highly purified human B- and T-lymphocytes. In unstimulated G0/G1 B- and T-lymphocytes the clastogen induction of chromosome fragments was investigated in prematurely condensed chromosomes (PCC) induced by cell fusion with xenogenic mitotic cells. BM, CP (with metabolic activation), and EMS induced a significant increase in chromosome aberrations in proliferating human B- and T-lymphocytes. There were no significant differences in the BM-induced aberration rates between the cell populations. CP and EMS induced more aberrations in T- than in B-lymphocytes. In the PCC tests, BM-exposed G0/G1 lymphocytes showed dose-dependent high yields of chromosome fragments. No significant differences between B- and T-lymphocytes were observed. CP and EMS induced no clear increase in fragments in either cell population.
Miller
K
K
Abteilung Humangenetik der Medizinischen Hochschule Hannover, F.R.G.
D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D001761:Bleomycin / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003520:Cyclophosphamide / Q000633:toxicity*; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D006801:Humans; D007621:Karyotyping; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D013223:Statistics as Topic; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*
D009153:Mutagens; D001761:Bleomycin; D003520:Cyclophosphamide; D005020:Ethyl Methanesulfonate