[Diversity and relative inaccuracy of internal images of antigen group A revealed by IEF analysis].
The diversity of a polyclonal anti-idiotypic response (Ab2) to a murine monoclonal anti-A (Ab1) was investigated after purification of two Ab2 populations. One was eluted from human polyclonal anti-A column and the other from Ab1. Analysis of the Ab specificity, as well as screening of the clonotypic distribution, were achieved after splitting Ab by IEF; this was followed by immunoblotting and probing with various anti-ABH mAb. The first population reacted with almost all the murine anti-ABH mAb, as well as with four human anti-A mAb, and consequently consisted of Ab2 beta. The second was composed of "true" Ab2 directed against Ab1. In the first population internal images mimicked either A Ag, or H Ag, or some epitopes common to both. This study demonstrates the plurality of internal images-bearing Ig molecules, some mimicking completely, and some only partially or even unfaithfully the nominal A determinant. The analysis of this idiotypic cascade proves the existence of a degeneracy of the initial restricted antigenic specificity. The consequences of such a process are discussed.
Crespeau
H
H
Institut national de Transfusion sanguine, Paris.
Alliot
C
C
Fine
J M
JM
Rochu
D
D
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D000939:Epitopes / Q000276:immunology* / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000276:immunology; D007525:Isoelectric Focusing; D051379:Mice; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007075:Immunoglobulin M
Diversité et infidélité relative des images internes de l'antigène de groupe A révélées par l'analyse par IEF.