title: Heterocycles related to nucleotides. XI. An organomercurial column chromatography for thiouridine.
abstract: By the organomercurial column: chromagel or Sephadex coupled to p-aminophenylmercury chloride, 4- and 2-thiouridine were selectively separated from the mixtures with major nucleotides. Oligonucleotides containing 4-thiouridilic acid were also separated from the ribonuclease-T1 digests of unfractionated transfer ribonucleic acid of E. coli.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Sato
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Kanaoka
authors.forename: K
authors.initials: K
authors.lastname: Kimura
mesh_terms: D002850:Chromatography, Gel; D004926:Escherichia coli / Q000235:genetics; D015394:Molecular Structure; D009705:Nucleosides / Q000302:isolation & purification; D009711:Nucleotides / Q000302:isolation & purification; D009841:Oligonucleotides / Q000302:isolation & purification; D010663:Phenylmercury Compounds; D012329:RNA, Bacterial / Q000302:isolation & purification; D012343:RNA, Transfer / Q000302:isolation & purification; D013891:Thiouridine / Q000302:isolation & purification*
chemical_list: D009705:Nucleosides; D009711:Nucleotides; D009841:Oligonucleotides; D010663:Phenylmercury Compounds; D012329:RNA, Bacterial; D013891:Thiouridine; C067422:4-aminophenylmercury chloride; D012343:RNA, Transfer