title: Molecular form of immunoreactive endothelin in plasma and urine of normal subjects and patients with various disease states.
abstract: To elucidate the pathophysiologic significance of the family of endothelin (ET) peptides, we have investigated plasma and urinary immunoreactive (ir-) ET levels and its molecular forms in normal and pathological conditions. Plasma and urine ET were extracted with an Amprep C2 column. The molecular form of ET was determined by a combination of radioimmunoassay and reverse-phase high-performance liquid chromatography. Although plasma ir-ET was composed mainly of big ET and endothelin-1 (ET-1) in normal subjects, that in acute myocardial infarction, chronic renal failure (CRF), essential hypertension, and vasospastic angina pectoris was characterized by an increase of high molecular ir-ET in addition to increases in big ET and ET-1. Urinary ir-ET in both normal subjects and patients with CRF was composed mainly of a high molecular form in addition to big ET and ET-1. These results suggest that the biosynthetic and/or degradation process of ET under pathological conditions appears to be different from that under normal conditions.
authors.affiliation: Department of Medicine, Tokyo Women's Medical College, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Naruse
authors.forename: M
authors.initials: M
authors.lastname: Naruse
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: I
authors.initials: I
authors.lastname: Yoshihara
authors.forename: K
authors.initials: K
authors.lastname: Ohsumi
authors.forename: J
authors.initials: J
authors.lastname: Horiuchi
authors.forename: C
authors.initials: C
authors.lastname: Monzen
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: N
authors.initials: N
authors.lastname: Sugino
mesh_terms: D000787:Angina Pectoris / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000378:metabolism*; D019332:Endothelin-1; D016232:Endothelins / Q000097:blood / Q000737:chemistry* / Q000652:urine; D006801:Humans; D006973:Hypertension / Q000378:metabolism; D007674:Kidney Diseases / Q000378:metabolism*; D009203:Myocardial Infarction / Q000378:metabolism; D011498:Protein Precursors / Q000737:chemistry
chemical_list: D019332:Endothelin-1; D016232:Endothelins; D011498:Protein Precursors