Selective effect of inhibitors on inner mitochondrial membrane channels.
The effect of amphiphilic cationic drugs on the channel activity of the mitochondrial inner membrane was examined with patch-clamp techniques. The therapeutic drugs amiodarone, propranolol and quinine reduced the probability of being open for the multiconductance channel (MCC) activity (levels from 30 pS to over 1 nS). While amiodarone decreased the probability of being open for the voltage dependent approximately 100 pS channel, it increased the conductance 42 +/- 20% (mean +/- SD, n = 6) with no significant change in mean open time. Similar results were obtained with propranolol. These data indicate that the approximately 100 pS channel is distinct from MCC activity.
Antonenko
Y N
YN
Department of Bioenergetics, A.N. Belozersky Laboratory, Moscow State University, USSR.
Kinnally
K W
KW
Perini
S
S
Tedeschi
H
H
D000638:Amiodarone / Q000494:pharmacology*; D000818:Animals; D004553:Electric Conductivity / Q000187:drug effects; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007473:Ion Channels / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology*; D011803:Quinine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D007473:Ion Channels; D011433:Propranolol; D011803:Quinine; D000638:Amiodarone