Diversity of T-cell antigen receptor variable genes used by mycosis fungoides cells.
The expression of T-cell antigen receptor beta-chain variable genes (V beta) was evaluated in 28 cases of mycosis fungoides. A novel polymerase chain reaction (PCR) technique was used to associate expression of particular V beta genes with monoclonal T-cell populations. In addition, the same biopsies used for PCR analysis were also examined for reactivity with a panel of seven monoclonal antibodies that specifically recognized V beta proteins from four different families. Only three cases clearly stained with the antibodies, a result consistent with a diverse set of V beta genes being used. This was confirmed by PCR analysis, which indicated that V beta genes from many different families were expressed by these tumors. Preferential use of the V beta 8 family, which had been previously use of the V beta 8 family, which had been previously reported for this disease, was not evident among the cases analyzed.
Bahler
D W
DW
Department of Medicine, Stanford University Medical Center, California.
Berry
G
G
Oksenberg
J
J
Warnke
R A
RA
Levy
R
R
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D001483:Base Sequence; D001706:Biopsy; D015139:Blotting, Southern; D004273:DNA, Neoplasm / Q000235:genetics; D005796:Genes / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009182:Mycosis Fungoides / Q000235:genetics* / Q000473:pathology; D009841:Oligonucleotides / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012867:Skin / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D009841:Oligonucleotides; D012334:RNA, Neoplasm; D011948:Receptors, Antigen, T-Cell