[The effect of salbutamol on histamine release from basophils in vivo and in vitro].
In vivo and in vitro basophil histamine release inhibition by salbutamol was investigated in patients with bronchial asthma. The study involved 14 patients in stable period of the disease: FEV1 = 66-84% of the normal values. Histamine release was determined following an incubation of the isolated basophils with concanavalin A (Sigma Co., USA) or specific allergens (dust, grass pollens, mites; Bencard, UK). Histamine was assayed with isotope-enzymatic technique according to Shaff and Beaven with histamine N-methyltransferase. Salbutamol administered intravenously in the dose of 1 mg inhibited allergen-induced histamine release from the basophils isolated from patient's blood within 30 minutes. Salbutamol in the concentration of 8 X 10(-6) M inhibited in vitro histamine release induced by an allergen and concanavalin A.
Chazan
R
R
Kliniki Pneumonologii Instytutu Chorób Wewnetrznych AM w Warszawie.
Wałajtys-Rode
E
E
Droszcz
P
P
D000328:Adult; D000420:Albuterol / Q000627:therapeutic use*; D001249:Asthma / Q000188:drug therapy*; D001491:Basophils / Q000378:metabolism*; D005260:Female; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged
D000420:Albuterol
Wpływ salbutamolu na uwalnianie histaminy z granulocytów zasadochłonnych krwi obwodowej w warunkach in vivo i in vitro.