Elimination of matrix effects in electrothermal atomic absorption spectrophotometric determinations of bismuth in serum and urine.
A sensitive and precise electrothermal atomic absorption spectrophotometric method for determining bismuth concentration is described. Protein precipitation and the use of a palladium modifier reduce the problems of foaming and permit the use of a higher ashing temperature. The detection limit of the assay is 0.9 nmol/L. Total CVs (intra- and interassay) for serum ranged from 3.5% to 15.1% and for urine from 4.8% to 14.5% at concentrations of 60.0 and 6.0 nmol/L, respectively. Analytical recoveries of bismuth added to serum and urine were 102% and 103% over the same range. The method is robust and reproducible and can be accurately calibrated with aqueous standards.
Dean
S
S
Department of Biochemistry, Royal Perth Hospital, Western Australia.
Tscherwonyi
P J
PJ
Riley
W J
WJ
D000328:Adult; D001729:Bismuth / Q000097:blood* / Q000652:urine*; D011232:Chemical Precipitation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010165:Palladium; D012016:Reference Values; D013054:Spectrophotometry, Atomic / Q000379:methods* / Q000706:statistics & numerical data; D013696:Temperature
D010165:Palladium; D001729:Bismuth