Cloning and sequence analysis of a cDNA encoding human non-selective type of endothelin receptor.
A cDNA encoding non-selective type (ETB) of endothelin receptor was isolated from a human liver cDNA library. The cDNA had an open reading frame encoding a protein of 442 amino acid residues with a relative Mr of 49,643. The deduced amino acid sequence of human ETB receptor was 88% and 64% identical to those of rat lung ETB receptor and bovine lung ET-1-specific (ETA) receptor, respectively, and contained a relatively long and proline-rich extracellular N-terminal region in addition to a significant similarity with the G protein-coupled receptor super-family with seven transmembrane segments.
Nakamuta
M
M
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Japan.
Takayanagi
R
R
Sakai
Y
Y
Sakamoto
S
S
Hagiwara
H
H
Mizuno
T
T
Saito
Y
Y
Hirose
S
S
Yamamoto
M
M
Nawata
H
H
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D016232:Endothelins / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics*; D017466:Receptors, Endothelin; D012689:Sequence Homology, Nucleic Acid
D016232:Endothelins; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D011061:Poly A; D012313:RNA; D004247:DNA