Identification of phosphorylcholine containing antigens of Fasciola hepatica--successful tolerization against this epitope in experimental animals.
Phosphorylcholine containing antigens have been identified in the parasite Fasciola hepatica by immunoblotting and ELISA. Immunoblots probed with polyclonal and monoclonal antibodies indicate that the majority of antigens identified in both the immature and mature parasite contain both phosphorylcholine and non-phosphorylcholine epitopes. One antigen of 58 kDa appears to contain predominantly PC epitopes or at least this epitope is the major one responded to by host animals. Successful immunotolerization against the epitope PC was achieved by injecting the PC conjugate, ovalbumin PC, into neonatal rats. Immunotolerization against PC resulted in a 25% reduction in worm burden upon subsequent infection with Fasciola hepatica.
Sloan
T
T
Department of Zoology, University College Dublin, Belfield, Ireland.
Dooge
D
D
Joyce
P
P
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000302:isolation & purification*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology*; D015151:Immunoblotting; D010767:Phosphorylcholine / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000939:Epitopes; D010767:Phosphorylcholine