Helium-neon laser irradiation of rat liver mitochondria gives rise to a new subpopulation of mitochondria: isolation and first biochemical characterization.
An experiment was performed to isolate the small atypical mitochondria produced during the irradiation of normal mitochondria with an He-Ne laser. Rat liver mitochondria were irradiated with a low-power continuous-wave He-Ne laser (energy dose, 5 J cm-2), followed by isolation using a sucrose gradient. In the irradiated sample, two bands were observed, one corresponding to normal mitochondria and the other to atypical mitochondria. Certain biochemical features of the mitochondria were investigated: mitochondrial enzyme activity and the presence of DNA and RNA were demonstrated. Hybridization experiments carried out with labelled mitochondrial probes, containing the genes for cytochrome oxidase subunit I and 12S rRNA, confirmed the mitochondrial nature of the isolated RNA.
Greco
M
M
Centro di Studio sui Mitocondri e Metabolismo Energetico CNR, Bari, Italy.
Perlino
E
E
Pastore
D
D
Guida
G
G
Marra
E
E
Quagliariello
E
E
D000263:Adenylate Kinase / Q000378:metabolism / Q000528:radiation effects; D000818:Animals; D002458:Cell Fractionation; D004272:DNA, Mitochondrial / Q000235:genetics / Q000528:radiation effects; D005969:Glutamate Dehydrogenase / Q000378:metabolism / Q000528:radiation effects; D006371:Helium; D007770:L-Lactate Dehydrogenase / Q000378:metabolism / Q000528:radiation effects; D007834:Lasers; D008291:Malate Dehydrogenase / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects*; D009356:Neon; D012313:RNA / Q000528:radiation effects; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic / Q000528:radiation effects
D004272:DNA, Mitochondrial; D006371:Helium; D009356:Neon; D012313:RNA; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005969:Glutamate Dehydrogenase; D000263:Adenylate Kinase