Identification of phosphotyrosine residues during protein sequence analysis.
Synthetic tyrosine-phosphorylated peptides were subjected to protein sequence analysis using a gas-phase sequencer and on-line phenylthiohydantoin (PTH) amino acid analysis. Our data show that phosphotyrosine is stable to the gas-phase sequencing chemistry and can be detected as its PTH-derivative during routine sequence analysis without the need of prior tyrosine radiolabeling.
Turck
C W
CW
Howard Hughes Medical Institute, University of California, San Francisco.
Herrmann
J
J
Escobedo
J A
JA
Williams
L T
LT
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010669:Phenylthiohydantoin; D019000:Phosphotyrosine; D011506:Proteins / Q000737:chemistry*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D011506:Proteins; D010669:Phenylthiohydantoin; D019000:Phosphotyrosine; D014443:Tyrosine