Tissue- and development-specific expression of HBGF-1 mRNA.
The gene for heparin-binding growth factor-1 (HBGF-1) encodes a 15.5-18 kDa polypeptide that affects the proliferation and differentiation of a broad range of mammalian cells and is widely distributed among normal adult tissues. In this study, we show that normal tissues of the adult rat express HBGF-1 transcripts in one of three patterns: a 4.4 kb mRNA was the predominant HBGF-1 transcript in brain, heart and lung; a 1.4 kb mRNA was the predominant transcript in the liver; approximately equal levels of the 1.4 and 4.4 kb mRNAs were found in the kidney. HBGF-1 expression was localized in two tissues: central nervous system expression of HBGF-1 was significantly higher in the brain stem compared to the cerebrum and cerebellum; renal expression of HBGF-1 was significantly higher in the medulla compared to the cortex. Analysis of the postnatal changes in HBGF-1 expression using the newborn rat kidney revealed that the level of HBGF-1 mRNA is low at birth and does not rise to adult levels until the seventh postnatal day. These findings demonstrate that HBGF-1 expression is specific for tissue type and stage of development.
Sullivan
D E
DE
Department of Pediatrics, Tulane University Medical Center, New Orleans, LA 70112.
Storch
T G
TG
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D004285:Dogs; D016220:Fibroblast Growth Factor 1 / Q000235:genetics*; D005786:Gene Expression Regulation; D006321:Heart / Q000254:growth & development; D007668:Kidney / Q000254:growth & development* / Q000378:metabolism; D008099:Liver / Q000254:growth & development / Q000378:metabolism; D008168:Lung / Q000254:growth & development / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
D012333:RNA, Messenger; D016220:Fibroblast Growth Factor 1