Murine eosinophil granulocytes bind the murine macrophage-monocyte specific monoclonal antibody F4/80.
Studies designed to identify a panel of monoclonal antibodies useful for the separation of murine eosinophil myeloid progenitors revealed that F4/80, an antigen heretofore thought to be expressed only by murine monocyte/macrophage lineage cells, was expressed by eosinophil granulocytes. Eosinophils from several strains of mice stained positively with specific antibody for the epitope. A novel pathway for myeloid differentiation is proposed in which neutrophil progenitors exit a common lineage prior to a common progenitor of monocytes and eosinophils. Moreover, the results demonstrate that binding of anti-F4/80 can no longer be viewed as exclusive for mononuclear phagocytes.
McGarry
M P
MP
Department of Laboratory Animal Resources, Roswell Park Cancer Institute, Buffalo, New York 14263.
Stewart
C C
CC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism*; D001666:Binding Sites, Antibody; D004804:Eosinophils / Q000378:metabolism* / Q000648:ultrastructure; D000939:Epitopes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D009000:Monocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes