Immunocytochemical study of tissues from clinically normal dogs and of neoplasms, using keratin monoclonal antibodies.
Three commonly used keratin monoclonal antibodies (MAB)--AE1:AE3, CAM 5.2, and MAK-6--were compared with routinely used cytokeratin antibody. The expression of these antibodies was analyzed in several tissues obtained from clinically normal dogs and in a variety of neoplasms from dogs. Using appropriate enzymatic digestion, paraffin-embedded tissues processed in routine manner retained their typical keratin expression. Differentiated and poorly differentiated epithelial neoplasms, lymphomas, and melanomas were studied by use of the avidin-biotin-peroxidase technique. All 4 of the aforementioned antibodies had similar staining profiles. Of 3 anaplastic carcinomas, 2 had positive reaction to all 4 antibodies. All lymphomas, plasma cell tumors, and amelanotic melanomas had negative reaction to MAK-6, CAM 5.2, AE1:AE3, and cytokeratin MAB. Three basal cell epitheliomas had positive reaction to all 4 antibodies, whereas 1 basal cell tumor with a solid pattern had negative staining reaction. Two carcinoids had negative reaction to all markers and 1 of 2 malignant chemodectomas and 1 transitional cell carcinoma had staining reaction to only AE1:AE3 MAB. Comparing the 4 antibodies, use of AE1:AE3 MAB produced the strongest staining intensity followed by cytokeratin, MAK-6, and CAM 5.2 MAB. All 4 antibodies had low background staining. In conclusion, AE1:AE3 and MAK-6 MAB are as useful as cytokeratin MAB for identification of poorly differentiated epithelial neoplasms in dogs and cats.
Sandusky
G E
GE
Toxicology Division, Lilly Research Laboratories, Greenfield, IN 46140.
Wightman
K A
KA
Carlton
W W
WW
D000818:Animals; D000911:Antibodies, Monoclonal; D002277:Carcinoma / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary; D004283:Dog Diseases / Q000175:diagnosis / Q000473:pathology*; D004285:Dogs; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000737:chemistry; D007633:Keratins / Q000032:analysis / Q000276:immunology*; D008175:Lung Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D011237:Predictive Value of Tests; D001743:Urinary Bladder / Q000737:chemistry
D000911:Antibodies, Monoclonal; D007633:Keratins