title: Anti-phosphotyrosine and anti-phosphoserine antibodies in SLE sera.
abstract: By using an ELISA method, we identified antiPTyr and antiPSer antibodies in the sera of some SLE patients. Afterwards, antiPTyr and antiPSer antibodies were purified by affinity chromatography on phosphotyramine-CNBr-Sepharose column and on phosphoethanolamine-CNBr-Sepharose, respectively, and the specificity of the purified antibodies was demonstrated by inhibition assays. The study pointed out a higher incidence of antiPTyr than anti PSer antibodies in the sera of these patients, which suggests that some "autoantigens" from membrane might be involved.
authors.affiliation: Cantacuzino Institute, Bucharest, Romania.
authors.forename: M
authors.initials: M
authors.lastname: Stefănescu
authors.forename: L
authors.initials: L
authors.lastname: Cremer
authors.forename: C
authors.initials: C
authors.lastname: Matache
authors.forename: C
authors.initials: C
authors.lastname: Cristescu
authors.forename: G
authors.initials: G
authors.lastname: Szegli
mesh_terms: D000906:Antibodies / Q000097:blood* / Q000302:isolation & purification; D001323:Autoantibodies / Q000097:blood; D002846:Chromatography, Affinity / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D010768:Phosphoserine / Q000276:immunology*; D019000:Phosphotyrosine; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000276:immunology
chemical_list: D000906:Antibodies; D001323:Autoantibodies; D010768:Phosphoserine; D019000:Phosphotyrosine; D014443:Tyrosine