Microinjection of neuropeptide Y into the superficial dorsal horn reduces stimulus-evoked release of immunoreactive substance P in the anaesthetized cat.
In barbiturate anaesthetized spinal cats, antibody microprobes were used to measure release of immunoreactive substance P in the superficial dorsal horn following electrical stimulation of unmyelinated primary afferents of the ipsilateral tibial nerve. Prior microinjection of neuropeptide Y (0.2-0.6 microliters of 10(-5) mol/l solution) in the region of the substantia gelatinosa reduced the evoked release of immunoreactive substance P for up to 40 min. Microinjection of similar volumes of phosphate-buffered saline at similar sites was without effect. This action of neuropeptide Y could contribute to analgesia, particularly if this neuropeptide is co-released with noradrenaline from axon terminals in the superficial dorsal horn.
Duggan
A W
AW
Department of Preclinical Veterinary Science, University of Edinburgh, U.K.
Hope
P J
PJ
Lang
C W
CW
D000344:Afferent Pathways / Q000187:drug effects / Q000502:physiology; D000768:Anesthesia, General; D000818:Animals; D002415:Cats; D003655:Decerebrate State; D004558:Electric Stimulation; D008845:Microinjections; D009478:Neuropeptide Y / Q000008:administration & dosage / Q000494:pharmacology*; D013373:Substance P / Q000378:metabolism*; D013376:Substantia Gelatinosa / Q000187:drug effects* / Q000502:physiology; D013979:Tibial Nerve / Q000502:physiology
D009478:Neuropeptide Y; D013373:Substance P