Human cells (normal and ataxia telangiectasia) transfected with pR plasmid are hypersensitive to DNA strand-breaking agents.
Ataxia telangiectasia (AT) cells are known to be hypersensitive to ionizing radiations and to drugs such as bleomycin and epipodophyllotoxin VP16, a topoisomerase II poison. Both of these produce DNA double-strand breaks even if through different mechanisms. In this work we analyzed the sensitivity to bleomycin and to epipodophyllotoxin of AT cells after transfection with pR plasmid. This plasmid, interacting with bacterial SOS repair pathways, expresses itself in mammalian cells conferring cell resistance to the SOS inducers UV and 4NQO and cell sensitivity to different drugs such as bleomycin. This effect is presumably due to the interaction of pR products with double-strand breaks. Our findings indicate that pR plasmid, in both AT lines tested (AT5BIVA fibroblasts and ATL6 lymphoblasts), expresses itself (increasing UV protection) and amplifies the already enhanced AT cell sensitivity to both bleomycin and VP16.
Antonelli
A
A
Dipartimento di Biopatologia Umana, Università La Sapienza, Rome, Italy.
Elli
R
R
Marcucci
L
L
Bosi
R
R
Kobal
D
D
Petrinelli
P
P
D001260:Ataxia Telangiectasia / Q000235:genetics*; D001761:Bleomycin / Q000494:pharmacology*; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000235:genetics; D002869:Chromosome Aberrations; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D005047:Etoposide / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D004854:Herpesvirus 4, Human; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects; D010957:Plasmids / Q000235:genetics; D013014:SOS Response, Genetics; D014162:Transfection
D001761:Bleomycin; D005047:Etoposide