Riboflavin carrier protein from carp (C. carpio) eggs: comparison with avian riboflavin carrier protein.
A protein exhibiting immunological cross-reactivity with the chicken egg-white riboflavin carrier protein was detected by radioimmunoassay in the eggs and serum of the fresh water fish Cyprinus carpio and subsequently purified to homogeneity by use of affinity chromatography. Fish riboflavin carrier protein resembled chicken riboflavin carrier protein with respect to most of its physicochemical characteristics. The major epitopes of chicken riboflavin carrier protein were shown to be conserved in the fish protein as probed with monoclonal antibodies to the avian vitamin carrier.
Malhotra
P
P
Centre for Reproductive Biology and Molecular Endocrinology, Indian Institute of Science, Bangalore.
Karande
A A
AA
Prasadan
T K
TK
Adiga
P R
PR
D000818:Animals; D000911:Antibodies, Monoclonal; D005075:Biological Evolution; D015152:Blotting, Northern; D015153:Blotting, Western; D002347:Carps / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D002645:Chickens; D002846:Chromatography, Affinity; D003429:Cross Reactions / Q000276:immunology; D015342:DNA Probes; D004527:Egg Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D026901:Membrane Transport Proteins; D011863:Radioimmunoassay; D012256:Riboflavin
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D015342:DNA Probes; D004527:Egg Proteins; D000939:Epitopes; D026901:Membrane Transport Proteins; C019079:riboflavin-binding protein; D012256:Riboflavin