Binding sites of the antibiotics pactamycin and celesticetin on ribosomal RNAs.
The binding sites of the antibiotics pactamycin and celesticetin on the rRNAs of Escherichia coli ribosomes were investigated by a chemical footprinting procedure. Pactamycin protected residues G-693 and C-795 in 16S RNA which are located in an important functional region of the 30S subunit participating in initiation complex formation and ribosomal subunit interaction. Celesticetin altered the reactivities of 5 residues A-2058, A-2059, A-2062, A-2451 and G-2505 within the central loop of domain V of 23S RNA which has been implicated in peptidyltransferase activity. Inferences are drawn concerning the mode of action of the antibiotics.
Egebjerg
J
J
Biostructural Chemistry, Aarhus University, Denmark.
Garrett
R A
RA
D000900:Anti-Bacterial Agents / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008034:Lincomycin / Q000031:analogs & derivatives / Q000378:metabolism; D055231:Lincosamides; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010142:Pactamycin / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D012338:RNA, Ribosomal, 23S / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D055231:Lincosamides; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D010142:Pactamycin; C004650:celesticetin A; D008034:Lincomycin