Estimation of the chloramphenicol and cycloheximide inhibition of protein synthesis in brain cholinergic synaptosomes.
Cholinergic synaptosomes have been prepared from sheep brain cortex by means of an immunoaffinity method using a specific anti-(Chol I) antiserum. The [14C]leucine incorporation into proteins of this preparation shows a low cycloheximide and a high chloramphenicol sensitivity. This fact suggests that the mitochondrial protein synthesis system is the only one present in this fraction.
Corbaton
V
V
Departamento de Bioqumica, Biología Molecular y Celular, Facultad de Veterinaria, Zaragoza, Spain.
Muiño
M T
MT
Fernández-Silva
P
P
López-Pérez
M J
MJ
Montoya
J
J
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology*; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010275:Parasympathetic Nervous System / Q000187:drug effects / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D012756:Sheep; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D012313:RNA; D002701:Chloramphenicol; D003513:Cycloheximide; D007930:Leucine