Anti-phosphotyrosine antibodies--obtaining and purification.
Anti-phosphotyrosine antibodies were obtained by rabbits immunization using bovine serum albumin-phosphotyrosine complex. Hyperimmune rabbit serum was purified on affinity chromatography column obtained by phosphotyramine binding on Sepharose 4 B, CNBr-activated. The antibodies obtained after purification were tested by ELISA and immunoblotting.
Stefănescu
M
M
Cantacuzino Institute, Bucharest, Romania.
Cremer
L
L
Cristescu
C
C
Matache
C
C
Szegli
G
G
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification*; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007114:Immunization; D015151:Immunoblotting / Q000379:methods; D019000:Phosphotyrosine; D011817:Rabbits; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000276:immunology
D000906:Antibodies; D019000:Phosphotyrosine; D014443:Tyrosine