title: Formation of cation channels in planar lipid bilayers by brefeldin A.
abstract: Brefeldin A (BFA) is a novel agent with the unique property of effecting a rapid increase of Golgi cisternae volume and subsequent loss of a recognizable Golgi apparatus in treated cells. Although a receptor-mediated mechanism has been proposed, the molecular basis of BFA action remains unknown (Lippincott-Schwartz, J., Glickman, J., Donaldson, J. G., Robbins, J., Kreis, T. E., Seamon, K. B., Sheetz, M. P., and Klausner, R. D. (1991) J. Cell Biol. 112, 567-577). Since a variety of ionophores distort Golgi architecture by initially causing osmotic swelling of the cisternae (Mollenhauer, H. H., Morre, D. J., and Rowe, L. D. (1990) Biochim. Biophys. Acta 1031, 225-246), Golgi membrane permeabilization by BFA seemed possible. We examined the effects of BFA on the conductance of planar lipid bilayers bathed in several aqueous salt solutions. Addition of BFA (1 microgram/ml) quickly augmented alkali cation conductance (K+ greater than Na+ much greater than Li+) but not anion conductance of the bilayer. Lower concentrations (1 ng/ml) indicated that BFA formed discrete, cation-selective channels in these bilayers. Given that Golgi cisternae volume increases immediately upon treatment with BFA, these findings suggest that alteration of ion gradients or Golgi membrane potential followed by an influx of water may be the mechanism by which BFA initiates disruption of Golgi structural integrity. Subsequent functional perturbations may then ensue either as a consequence of these initial structural changes or by a combination of several distinct mechanisms.
authors.affiliation: Department of Nephrology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
authors.forename: M
authors.initials: M
authors.lastname: Zizi
authors.forename: R S
authors.initials: RS
authors.lastname: Fisher
authors.forename: F G
authors.initials: FG
authors.lastname: Grillo
mesh_terms: D020126:Brefeldin A; D002412:Cations; D003517:Cyclopentanes / Q000494:pharmacology*; D004563:Electrochemistry; D007473:Ion Channels / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology*; D008051:Lipid Bilayers / Q000378:metabolism*
chemical_list: D002412:Cations; D003517:Cyclopentanes; D007473:Ion Channels; D007476:Ionophores; D008051:Lipid Bilayers; D020126:Brefeldin A