Bovine immunodeficiency virus: immunochemical characterization and serological survey.
Bovine immunodeficiency virus (BIV) was purified by isodensity centrifugation; viral activities were monitored in gradient fractions using the reverse transcriptase assay and a p26-specific monoclonal antibody ELISA. In the coincident peak fractions (density about 1.17 g/ml) proteins with Mr values of 26K, 17K, 53K, 14K and 100K (with decreasing intensity) were detected by Western blotting using serum of a calf after experimental BIV infection. When 957 randomly collected cattle sera from The Netherlands were tested by indirect immunofluorescence and confirmed using Western blot and/or radioimmunoprecipitation, 1.4% appeared seropositive. Thus BIV infection is not uncommon in one European cattle population.
Horzinek
M
M
Department of Virology, Veterinary Faculty, State University of Utrecht, The Netherlands.
Keldermans
L
L
Stuurman
T
T
Black
J
J
Herrewegh
A
A
Sillekens
P
P
Koolen
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D016087:Immunodeficiency Virus, Bovine / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D009426:Netherlands / Q000453:epidemiology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D015531:Radioimmunoprecipitation Assay / Q000662:veterinary; D012698:Serologic Tests
D000911:Antibodies, Monoclonal; D012194:RNA-Directed DNA Polymerase