Alpha interferon suppresses virion but not soluble human immunodeficiency virus antigen production in chronically infected T-lymphocytic cells.
Alpha interferon (IFN-alpha) is effective in preventing the release of human immunodeficiency virus (HIV) from chronically infected T-lymphocytic (ACH-2) and promonocytic (U1) cell lines stimulated with the phorbol ester phorbol-12-myristate-13 acetate (PMA). In the present study, we observed that together with particle production, shedding of HIV antigen (p24gag) occurs in the T-cell line ACH-2 both constitutively and after stimulation with PMA. IFN-alpha, although effective in suppressing the release of HIV particles, did not inhibit shedding of p24gag into the culture supernatants of either unstimulated or PMA-stimulated cells. These observations may be of relevance in the evaluation of the in vivo efficacy of IFN-alpha treatment of HIV-infected individuals as determined by levels of p24 antigen in plasma.
Fernie
B F
BF
Department of Microbiology, Georgetown University, Rockville, Maryland 20852.
Poli
G
G
Fauci
A S
AS
D002460:Cell Line; D015683:Gene Products, gag / Q000276:immunology / Q000378:metabolism; D015488:HIV Antigens / Q000096:biosynthesis* / Q000737:chemistry; D016655:HIV Core Protein p24; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D008213:Lymphocyte Activation; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012995:Solubility; D013601:T-Lymphocytes / Q000382:microbiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014758:Viral Core Proteins / Q000276:immunology / Q000378:metabolism; D014771:Virion / Q000276:immunology
D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D007370:Interferon Type I; D014758:Viral Core Proteins; D012194:RNA-Directed DNA Polymerase; D013755:Tetradecanoylphorbol Acetate