[Granulocyte differentiation of human promyelocytic leukemic cells induced by coordinate action of granulocyte colony stimulating factor and retinoic acid].
We analysed the effects of recombinant human G-CSF (rhG-CSF) and retinoic acid (RA) on proliferation and differentiation of HL-60 cells and human acute myeloid leukemic (AML) cells. A synergistic effect on granulocyte differentiation was observed when HL-60 cells and primary cultured acute promyelocyte leukemic cells were cocultured with 10(-8)mol/L RA plus 1:2000 or 1:1000 rhG-CSF. The rhG-CSF plus RA treated cells demonstrated significant increase in the percentage of mature cells. Morphological changes and nitroblue tetrazolium (NBT) reduction activity evidenced more increase than RA treatment alone (P less than 0.001). The results suggest that RA not only inhibits the proliferative action of G-CSF, but also retains and enhances the action of G-CSF to induce differentiation. Therefore, we believe that the combined use of G-CSF with RA may improve the treatment of leukemia.
Huang
F
F
D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology; D015479:Leukemia, Myelomonocytic, Acute / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D014212:Tretinoin