Phenotypic abnormalities in CD8+ T lymphocyte subsets in patients with rheumatoid arthritis.
We analyzed the cell surface phenotype of CD8+ cells in both peripheral blood and synovial fluid (SF) of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Utilizing the monoclonal antibodies anti-CD45RA, anti-CD29 and anti-S6F1-, one can define both suppressor effector (CD45RA+CD29-S6F1-) and killer effector (CD45RA-CD29+S6F1+) cells within the CD8 population. In patients with OA, normal proportions of CD8+CD45RA+, CD8+CD29+ and CD8+S6F1+ cells were found in both peripheral blood and SF. The peripheral blood of patients with RA, in contrast, showed a decreased percentage of CD8+CD45RA+ cells (13.4 +/- 2.6) (p less than 0.05), but a normal percentage of CD8+CD29+ and CD8+S6F1+ cells. In the SF of patients with RA, we observed a more dramatic decrease in CD8+CD45RA+ suppressor effector cells (6.4 +/- 5.0) (p less than 0.001), a significant increase in killer effector cells as measured by both CD8 + CD29+ (35.5 +/- 9.9) (p less than 0.001) and CD8 + S6F1+ cells (28.2 +/- 11.4) (p less than 0.01). These changes may contribute to the immunologic abnormalities previously noted in this disease and may provide some insight into the pathophysiologic mechanisms of RA.
Sohen
S
S
Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School.
Romain
P L
PL
Rothstein
D M
DM
Yamane
T
T
Tanaka
S
S
Anderson
P
P
Schlossman
S F
SF
Morimoto
C
C
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000276:immunology*; D016827:CD8 Antigens / Q000032:analysis*; D005260:Female; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D019012:Integrin beta1; D017493:Leukocyte Common Antigens; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D013582:Synovial Fluid / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D015703:Antigens, CD; D016827:CD8 Antigens; D006649:Histocompatibility Antigens; D019012:Integrin beta1; D017493:Leukocyte Common Antigens