title: Evaluation of three techniques to demonstrate retrograde transport of horseradish peroxidase.
abstract: Three methods of tracing neural connections by the use of horseradish peroxidase (HRP) were evaluated: (1) endoneurium injection, (2) injection followed by crushing at site of injection, and (3) nerve transection followed by capping of the proximal stump with a silicone cylinder containing HRP. The capping technique resulted in increased uptake and more uniform distribution of HRP in the nerves under study.
authors.affiliation: University of Pennsylvania, School of Veterinary Medicine, New Bolton Center, Kennett Square 19348.
authors.forename: J A
authors.initials: JA
authors.lastname: Orsini
authors.forename: R V
authors.initials: RV
authors.lastname: Pollock
mesh_terms: D000818:Animals; D001370:Axonal Transport; D001693:Biological Transport, Active; D004285:Dogs; D006735:Horseradish Peroxidase / Q000008:administration & dosage / Q000493:pharmacokinetics*; D009498:Neurotoxins / Q000493:pharmacokinetics; D010525:Peripheral Nerves / Q000378:metabolism*
chemical_list: D009498:Neurotoxins; D006735:Horseradish Peroxidase