title: Generation and characterization of two monoclonal self-associating IgG rheumatoid factors from a rheumatoid synovium.
abstract: OBJECTIVE
  To study IgG rheumatoid factor (RF) from rheumatoid synovium.
  
  
  METHODS
  We fused the K6H6/B5 human-mouse heterohybridoma with unstimulated rheumatoid synovial B cells to generate IgG-RF-secreting hybridomas.
  
  
  RESULTS
  The RFs from 2 such hybridomas bound specifically to the Fc fragment of human IgG and self-associated to form immune complexes. Such immune complexes are a major characteristic of the pathogenic IgG-RFs in rheumatoid synovium.
  
  
  CONCLUSIONS
  IgG-RF-secreting hybridomas have been obtained. Analyses may reveal the underlying mechanisms of the induction of IgG-RF.
authors.affiliation: Department of Medicine, University of California, San Diego 92093.
authors.forename: E W
authors.initials: EW
authors.lastname: Lu
authors.forename: M
authors.initials: M
authors.lastname: Deftos
authors.forename: H
authors.initials: H
authors.lastname: Tighe
authors.forename: D A
authors.initials: DA
authors.lastname: Carson
authors.forename: P P
authors.initials: PP
authors.lastname: Chen
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000473:pathology; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D012217:Rheumatoid Factor / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D013583:Synovial Membrane / Q000737:chemistry* / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D012217:Rheumatoid Factor