Beta-subunits of equine chorionic gonadotropin and lutenizing hormone with an identical amino acid sequence have different asparagine-linked oligosaccharide chains.
The glycoprotein hormones, equine chorionic gonadotropin (eCG) and lutenizing hormone (eLH), possess a beta-subunit with an identical amino acid sequence. The Asn-linked oligosaccharide chains of eCG beta and eLH beta were quantitatively liberated as tritium-labeled oligosaccharides by hydrazinolysis followed by N-acetylation and NaB3H4-reduction. Paper electrophoresis in combination with sialidase digestion and solvolytic desulfation indicated that eCG beta contained neutral and sialylated oligosaccharides, while eLH beta contained neutral, sialylated, sulfated, and both sialylated and sulfated oligosaccharides. In addition, elution profiles on a Bio-Gel P-4 column of the neutralized oligosaccharide mixtures of eCG beta and eLH beta were different, indicating that the molecular masses of oligosaccharides of the two glycoproteins are different. Therefore, this suggests that the structures of the Asn-linked oligosaccharide chains of eCG beta and eLH beta are different although they have an identical amino acid sequence.
Matsui
T
T
Division of Biomedical Polymer Science, Fujita Health University School of Medicine, Aichi, Japan.
Sugino
H
H
Miura
M
M
Bousfield
G R
GR
Ward
D N
DN
Titani
K
K
Mizuochi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001216:Asparagine; D006063:Chorionic Gonadotropin / Q000737:chemistry*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D002850:Chromatography, Gel; D004590:Electrophoresis, Paper; D006736:Horses; D007986:Luteinizing Hormone / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid; D012794:Sialic Acids / Q000032:analysis
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D009844:Oligosaccharides; D010446:Peptide Fragments; D012794:Sialic Acids; D001216:Asparagine; D007986:Luteinizing Hormone