Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation.
Hematoxylin-eosin staining (H&E) of cytomegalovirus (CMV) inclusion bodies was compared with in situ hybridization using a biotinylated DNA probe for the detection of CMV. Of 29 biopsy specimens selected from 23 patients with clinical CMV hepatitis and typical CMV inclusions on histopathologic examination, 23 (79%) were positive by DNA probe and 17 (59%) were detected in cell cultures. The mean number of CMV foci per tissue section was higher by DNA probe (12) compared with H&E (5). We do not recommend in situ hybridization in microbiology laboratories as a replacement for histopathology for the diagnosis of CMV in tissue specimens.
Espy
M J
MJ
Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905.
Paya
C V
CV
Holley
K E
KE
Ludwig
J
J
Hermans
P F
PF
Wiesner
R H
RH
Krom
R A
RA
Smith
T F
TF
D001707:Biopsy, Needle; D001710:Biotin; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D006525:Hepatitis, Viral, Human / Q000175:diagnosis*; D006801:Humans; D007181:Inclusion Bodies, Viral; D008099:Liver / Q000382:microbiology* / Q000473:pathology; D016031:Liver Transplantation; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D013194:Staining and Labeling
D015342:DNA Probes; D004279:DNA, Viral; D001710:Biotin