title: Expression of renal Na(+)-Ca2+ exchange activity in Xenopus laevis oocytes.
abstract: The expression of a renal Na(+)-Ca2+ exchanger by Xenopus oocytes has been investigated. Each oocyte was injected with 50 ng of poly(A)+ RNA from either rat or rabbit kidney or with an equivalent volume of water. Na(+)-Ca2+ exchange was determined 3 days after injection, by measuring Ca2+ uptake by oocytes in the presence or absence of an outwardly directed Na+ concentration gradient. To manipulate Na+ concentration gradients, oocytes were first loaded with Na+ in Ca(2+)-free medium containing 90 mM Na+ and nystatin. They were then exposed to medium containing 45Ca and either 90 mM or 0 Na+. Na(+)-free media contained (in mM) either 90 K+, 90 choline or 85 choline plus 5 K+. Oocytes injected with rat kidney poly(A)+ RNA showed a Na+ gradient-dependent Ca2+ uptake of 6.6 +/- 0.8 (SE, n = 5) pmol.oocyte-1.30 min-1. This is significantly higher than the value of 3.4 +/- 0.5 (SE, n = 5) pmol.oocyte-1.30 min-1 obtained in water-injected oocytes (P less than 0.001). Similar results were obtained using poly(A)+ RNA from rabbit kidney cortex. Neither 10 microM nifedipine nor 0.5 mM D 600 significantly affected this Ca2+ uptake. However, 90% of the Ca2+ uptake was inhibited in the presence of 0.1 mM La3+. The poly(A)+ RNA-induced Na(+)-Ca2+ exchange activity was stimulated by the presence of 5 mM K+ in the extracellular choline solution compared with choline alone. Fractionation experiments indicate that the rat kidney Na(+)-Ca2+ exchanger was encoded by poly(A)+ RNA of 3-4 kb.
authors.affiliation: Department of Physiology and Biophysics, Cornell University Medical College, New York, New York 10021.
authors.forename: S
authors.initials: S
authors.lastname: Milovanovic
authors.forename: G
authors.initials: G
authors.lastname: Frindt
authors.forename: S S
authors.initials: SS
authors.lastname: Tate
authors.forename: E E
authors.initials: EE
authors.lastname: Windhager
mesh_terms: D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism*; D005591:Chemical Fractionation; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D007267:Injections; D007668:Kidney / Q000378:metabolism; D007811:Lanthanum / Q000493:pharmacokinetics; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000235:genetics; D011188:Potassium / Q000378:metabolism; D012313:RNA / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D019831:Sodium-Calcium Exchanger; D014982:Xenopus laevis
chemical_list: D002121:Calcium Channel Blockers; D002352:Carrier Proteins; D019831:Sodium-Calcium Exchanger; D011061:Poly A; D012313:RNA; D007811:Lanthanum; D012964:Sodium; D011188:Potassium; D002118:Calcium