title: [Antibodies against hepatitis C virus. Experience with a second generation test].
abstract: A second generation recombinant immunoblot assay to detect antibodies against Hepatitis C virus (RIBA II, Ortho Diagnostic Systems) was applied to 30 serum samples repeatedly reactive to ELISA anti-HCV c100-3, 11 from hemodialysis patients, 11 from patients with chronic hepatitis and 8 from patients with cirrhosis. The assay detects individual antibodies directed to 4 antigenic components of the C virus, 2 antigens, c100 and 5-1-1 contained in the original ELISA assay and 2 new antigens, c33c and c22-3 the latter structural in nature. From the total of 30 samples, 23 (77%) were reactive, 4 (13%) indeterminate and 3 (10%) non reactive to the RIBA II assay. Much variation was observed regarding the response to each individual antigen. c22-3 was the most frequently reactive with the highest intensity. RIBA II assay confirmed a high percentage of the reactive results with the ELISA c-100-3, serum samples that were non reactive to RIBA showed a low optical density with ELISA. c22-3 was the most sensitive antigenic component.
authors.affiliation: Departamento de Gastroenterología, Inmunología y Nefrología, Hospital Militar Dr. Carlos Arvelo, Caracas.
authors.forename: M A
authors.initials: MA
authors.lastname: Garassini
authors.forename: F
authors.initials: F
authors.lastname: Ortega
authors.forename: M
authors.initials: M
authors.lastname: Alvarado
authors.forename: A
authors.initials: A
authors.lastname: Valdés
authors.forename: F
authors.initials: F
authors.lastname: Díaz
authors.forename: I
authors.initials: I
authors.lastname: Dugarte
authors.forename: L
authors.initials: L
authors.lastname: Serrano
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000032:analysis*; D006526:Hepatitis C / Q000175:diagnosis / Q000276:immunology*; D018937:Hepatitis C Antibodies; D006801:Humans; D015151:Immunoblotting; D012680:Sensitivity and Specificity
chemical_list: D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies
vernacular_title: Anticuerpos contra el virus de la hepatitis C. Experiencias con una prueba de segunda generación.