Is there a unique ribosome phenotype for naturally occurring Escherichia coli?
We have compared the growth characteristics of natural isolates of E coli with the kinetic properties of their ribosomes in vitro. The variability of the different performance characteristics of ribosomes isolated from natural isolates of E coli show that there is not a unique wild-type ribosome phenotype just as there is not a unique growth phenotype for the bacteria. In addition, there is a strong correlation between the growth rates and the efficiency of the kinetic interaction between the ribosomes and the EF-Tu-GTP-aminoacyl-tRNA ternary complex in vitro. No such correlation is seen between the growth rate and the maximum turnover rate of the ribosomes in vitro. These data suggest that the codon-programmed ribosomes are not kinetically saturated with ternary complexes in vivo.
Mikkola
R
R
Department of Molecular Biology, Uppsala University, Sweden.
Kurland
C G
CG
D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D010444:Peptide Elongation Factor Tu / Q000378:metabolism; D010641:Phenotype; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D006160:Guanosine Triphosphate; D010444:Peptide Elongation Factor Tu