Antiidiotypic antibodies interacting with cGMP-dependent channels of frog retinal rod outer segments.
Antiidiotypic approach was used to obtain antibodies interacting with cGMP-binding site of the cGMP-activated channel of the photoreceptor cell. Monoclonal anti-BrcGMP antibodies having characteristics of binding of agonist and its analogs close to those for a natural receptor have been obtained. These antibodies were used to raise polyclonal antiidiotypic antibodies capable of interacting with a natural cGMP-receptor. Application of immunoglobulins, isolated from antiidiotypic serum, to inside-out fragments of the rod plasma membrane led to an irreversible increase of the conductance of cGMP-dependent channels.
Krapivinsky
G B
GB
Laboratory of Biophysics of Reception, USSR Academy of Sciences, Moscow Region.
Filatova
E A
EA
Filatov
G N
GN
Jainazarov
A B
AB
Mantzygin YuA
Fesenko
E E
EE
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D006152:Cyclic GMP / Q000502:physiology*; D054815:Cyclic Nucleotide-Gated Cation Channels; D005136:Eye Proteins / Q000276:immunology*; D007106:Immune Sera / Q000737:chemistry; D007473:Ion Channels / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D011898:Ranidae; D012374:Rod Cell Outer Segment / Q000737:chemistry*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D054815:Cyclic Nucleotide-Gated Cation Channels; D005136:Eye Proteins; D007106:Immune Sera; D007473:Ion Channels; D006152:Cyclic GMP