Efficient method of haemoglobin F enrichment in adult haemolysate for determination of the gamma-chain isoforms by high-performance liquid chromatography.
An alkali denaturation procedure was developed for the efficient enrichment of haemoglobin F (Hb F) in human adult haemolysate. In contrast to the previous procedures, the method is readily applicable to small amounts of blood containing less than 1% of Hb F, providing reproducibly a sufficient level of Hb F (at least 11%) to allow an accurate high-performance liquid chromatographic determination of the three gamma-chain isoforms. Further enrichment was possible by combination with CM-Sephadex chromatography.
Enoki
Y
Y
Second Department of Physiology, Nara Medical University, Japan.
Ohga
Y
Y
Nagano
R
R
Matsumura
K
K
D000328:Adult; D000468:Alkalies / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005319:Fetal Hemoglobin / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D008024:Ligands; D011487:Protein Conformation / Q000187:drug effects; D013053:Spectrophotometry
D000468:Alkalies; D008024:Ligands; D005319:Fetal Hemoglobin