title: Small differences in Drosophila tropomyosin expression have significant effects on muscle function.
abstract: The effects of promoter deletions on Drosophila tropomyosin I (TmI) gene expression have been determined by measuring TmI RNA levels in transformed flies. Decreases in RNA levels have been correlated with rescue of flightless and jumpless mutant phenotypes in Ifm(3)3 mutant transformed flies and changes in muscle ultrastructure. The results of this analysis have allowed us to identify a region responsible for 20% of maximal TmI expression, estimate threshold levels of TmI RNA required for indirect flight and jump muscle function, and obtain evidence suggesting that sarcomere length may be an important determinant of flight muscle function.
authors.affiliation: Department of Biochemistry, University of Illinois College of Medicine, Chicago 60612.
authors.forename: T
authors.initials: T
authors.lastname: Tansey
authors.forename: J R
authors.initials: JR
authors.lastname: Schultz
authors.forename: R C
authors.initials: RC
authors.lastname: Miller
authors.forename: R V
authors.initials: RV
authors.lastname: Storti
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics; D005260:Female; D009132:Muscles / Q000502:physiology* / Q000648:ultrastructure; D009154:Mutation; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012313:RNA / Q000378:metabolism; D014170:Transformation, Genetic; D014335:Tropomyosin / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D014335:Tropomyosin; D012313:RNA