Decreased formation of inositol trisphosphate in Madin-Darby canine kidney cells under conditions of beta-glucosidase inhibition.
Recent work has demonstrated the enhancement of hormone-stimulated inositol trisphosphate formation in renal epithelial cells under conditions of glucosylceramide depletion. The role of glucosylceramide metabolism was explored further by exposing Madin-Darby canine kidney (MDCK) cells to the beta-glucosidase inhibitor conduritol B epoxide, which produced time-dependent and concentration-dependent increases in glucosylceramide levels and decreased bradykinin-stimulated inositol trisphosphate formation from isolated MDCK cell membranes. These data provide further support for an association between glucosylceramide levels and hormone-stimulated inositol trisphosphate formation.
Mahdiyoun
S
S
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0676.
Deshmukh
G D
GD
Abe
A
A
Radin
N S
NS
Shayman
J A
JA
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002460:Cell Line; D004285:Dogs; D005690:Galactose / Q000378:metabolism; D005963:Glucosylceramides / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology*; D007294:Inositol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007668:Kidney; D007700:Kinetics; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D011865:Radioisotope Dilution Technique; D013107:Sphingolipids / Q000378:metabolism; D014316:Tritium; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors*
D005963:Glucosylceramides; D010169:Palmitic Acids; D013107:Sphingolipids; D014316:Tritium; D019308:Palmitic Acid; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D007294:Inositol; D015544:Inositol 1,4,5-Trisphosphate; D001617:beta-Glucosidase; C011423:conduritol epoxide; D001920:Bradykinin; D005690:Galactose