Isolation and characterization of 1,200 kDa peptide of alpha-connectin.
When rabbit skeletal muscle myofibrils were kept for 12 h at 4 degrees C, alpha-connectin was partially degraded and 1,200 kDa peptide was newly formed [Takahashi, K. & Takai, H. (1988) Abst. 80th Jpn. Soc. Zootech. Sci. p-102]. The latter was isolated together with remaining alpha-connectin. Ultracentrifugation of the mixture at low ionic strength resulted in sedimentation of alpha-connectin, leaving the 1,200 kDa peptide in the supernatant. Physicochemical properties of the isolated 1,200 kDa peptide were investigated: UV absorption spectra, circular dichroism spectra, amino acid composition, and molecular size and shape. Polyclonal antibodies against the 1,200 kDa peptide [PcAb(1200)] bound the Z line and I-band. The position of the stripe in the I band near the N1 line due to the binding of PcAb(1200) moved both away from the Z lines and from the A band as sarcomeres were elongated. Therefore, it is considered that the 1,200 kDa portion of alpha-connectin is elastic.
Matsuura
T
T
Department of Biology, Faculty of Science, Chiba University.
Kimura
S
S
Ohtsuka
S
S
Maruyama
K
K
D000199:Actins / Q000302:isolation & purification; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies; D002942:Circular Dichroism; D064211:Connectin; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008565:Membrane Proteins / Q000737:chemistry; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry* / Q000302:isolation & purification; D009132:Muscles / Q000737:chemistry / Q000166:cytology; D009210:Myofibrils / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D011487:Protein Conformation; D011494:Protein Kinases; D011817:Rabbits
D000199:Actins; D000596:Amino Acids; D000906:Antibodies; D064211:Connectin; D000939:Epitopes; D008565:Membrane Proteins; D009124:Muscle Proteins; D010446:Peptide Fragments; D011494:Protein Kinases