[Detection of pneumococcal capsular polysaccharide antigen in urine by counterimmunoelectrophoresis. Technical features and correlation with serotype].
The effectiveness of CIE in detecting capsular polysaccharide antigen of pneumococci in urine is revised. Using CIE, we studied urine samples from 57 patients with systemic pneumococcal infections, proved bacteriologically by means of isolation of the microorganisms from sterile sites. We compare the usefulness of CIE with the microorganism isolation from organic products. We also determine the sensitivity gain after passive diffusion at 4 degrees C and bright blue Coomasie staining (CBB R-250). We correlate the CIE results with the serotype of isolated pneumococci. CIE was positive in 17 of all 35 pneumonia cases (48.6%), in 2 of all 5 bacteremias (40%), 4 of all 15 meningitis (26.7%) and in none of all two peritonitis. Direct urine examination was positive in 12 out of 23 patients with positive CIE (52.2%) and concentrated urine in 22 patients (95.6%). Passive diffusion at 4 degrees C and CBB R-250 stain increases the positive rate of the test. The correlation of serotype with our results is difficult due to the wide variety of serotypes identified. However, particular serotypes such (3, 4 or 8) had been identified with increased sensitivity. Global sensitivity of CIE in detecting capsular polysaccharide antigen in urine samples is not high enough (40.3%), even under the best circumstances. Antigen detection in urine is more sensitive than blood cultures, and therefore we believe that could be used in clinical microbiology laboratories until a more effective method is available.
Coll
P
P
Servicio de Microbiología, Hospital de la Santa Creu i Sant Pau, Barcelona.
Moreno
C
C
Sánchez
F
F
Navarro
F
F
Vilamala
A
A
Prats
G
G
D000942:Antigens, Bacterial / Q000652:urine*; D003080:Cold Temperature; D003378:Counterimmunoelectrophoresis; D006801:Humans; D011008:Pneumococcal Infections / Q000652:urine*; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000652:urine*; D012394:Rosaniline Dyes; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D013296:Streptococcus pneumoniae / Q000145:classification / Q000276:immunology*
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; D012394:Rosaniline Dyes; C048139:Coomassie blue
Detección de antígeno polisacárido capsular neumocócico en orina por contrainmunoelectroforesis. Aspectos técnicos y correlación con el serotipo.