Immortalization of human adult normal prostatic epithelial cells by liposomes containing large T-SV40 gene.
Simian virus SV40 has been widely used to immortalize epithelial cells of mammalian origin. We report here, for the first time to our knowledge, the immortalization of normal adult prostatic epithelial cells in culture by transfection of a plasmid containing SV40 genome with a defective replication origin (SV40 ori-) encapsulated into liposomes. These cells (PNT1) have now been cultured for more than 12 months, and shown to contain the SV40 genome. They express large T protein, present the phenotype of differentiated luminal prostatic cells (positive with antibodies to cytokeratin 18, 19, weakly positive for prostatic acid phosphatase and prostatic specific antigen, negative with anticytokeratin 14 and KL2 antibody). PNT1 cells contain high affinity receptors for dihydrotestosterone. These cells provide a useful tool to study the biology and the pathology of adult prostatic epithelial cells, specially to understand the steps leading to prostatic transformation.
Cussenot
O
O
Department of Urology, INSERM Unit 301, Hospital Saint-Louis, Paris, France.
Berthon
P
P
Berger
R
R
Mowszowicz
I
I
Faille
A
A
Hojman
F
F
Teillac
P
P
Le Duc
A
A
Calvo
F
F
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism; D000957:Antigens, Viral, Tumor / Q000032:analysis / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D007621:Karyotyping; D007633:Keratins / Q000378:metabolism; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011467:Prostate / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology; D017430:Prostate-Specific Antigen; D011944:Receptors, Androgen / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection; D014746:Vimentin / Q000378:metabolism
D000951:Antigens, Neoplasm; D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D011944:Receptors, Androgen; D014746:Vimentin; D007633:Keratins; D000135:Acid Phosphatase; D017430:Prostate-Specific Antigen