title: Preparation of antibacterial and antitoxic Clostridium difficile sera.
abstract: Preparation of Clostridium difficile antibacterial and antitoxic sera is presented. Fifty one strains (72%) were typeable within Delmee scheme. Twenty strains (28%) belonged to new Polish serogroups designated 18, 27, 70, 71, 72, 88, 89 and NICH. Supernatants of all toxigenic Clostridium difficile strains were neutralized by gamma-globulin fraction of goat Clostridium difficile antitoxin in neutralization assay when it was performed on McCoy cell line. Only 8 toxigenic strains (21%) were positive in counterimmunoelectrophoresis.
authors.affiliation: Department of Bacteriology and Immunology, Institute of Biostructure Medical Academy, Warsaw, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Sokół-Leszczyńska
authors.forename: G
authors.initials: G
authors.lastname: Martirossian
authors.forename: F
authors.initials: F
authors.lastname: Meisel-Mikołajczyk
authors.forename: J
authors.initials: J
authors.lastname: Mierzejewski
authors.forename: J
authors.initials: J
authors.lastname: Matras
authors.forename: D
authors.initials: D
authors.lastname: Lipnicki
authors.forename: W
authors.initials: W
authors.lastname: Sadowski
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood*; D000992:Antitoxins / Q000096:biosynthesis / Q000097:blood*; D016360:Clostridioides difficile / Q000145:classification / Q000276:immunology*; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis; D006041:Goats; D007106:Immune Sera / Q000096:biosynthesis*; D011817:Rabbits; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial; D000992:Antitoxins; D007106:Immune Sera