Expression, purification, and crystallization of the HIV-1 reverse transcriptase (RT).
The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.
Unge
T
T
Department of Molecular Biology, Uppsala University, Sweden.
Ahola
H
H
Bhikhabhai
R
R
Bäckbro
K
K
Lövgren
S
S
Fenyö
E M
EM
Honigman
A
A
Panet
A
A
Gronowitz
J S
JS
Strandberg
B
B
D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015751:Genes, pol; D015497:HIV-1 / Q000201:enzymology* / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
D012194:RNA-Directed DNA Polymerase