Identification and nucleotide sequence determination of the gene responsible for Ogawa serotype specificity of V. cholerae 01.
The gene encoding a protein of 27 kDa, which is specifically expressed in Vibrio cholerae of serotype Ogawa, was identified and its nucleotide sequence determined. The plasmid carrying this gene was found to convert serotype specificity from Inaba to Ogawa when introduced into the Escherichia coli DH5(pVCI112) cell which harbors a cloned 20-kilobase genomic DNA fragment of V. cholerae NIH35A3 and expresses the 01 antigen of Inaba serotype.
Ito
T
T
Department of Bacteriology, Faculty of Medicine, Juntendo University, Tokyo, Japan.
Ohshita
Y
Y
Hiramatsu
K
K
Yokota
T
T
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D019081:O Antigens; D015183:Restriction Mapping; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D019081:O Antigens