Establishment of an alpha-fetoprotein-producing gastric cancer cell line in serum-free media.
A new cell line, designated ISt-1, was established in serum-free medium from a 63-year-old gastric cancer patient with an extremely high serum level of alpha-fetoprotein (AFP). ISt-1 exhibited a typical morphology of epithelial cells in culture. The population doubling time was 31 hours. Production of AFP was demonstrated by immunohistochemical study and high levels of AFP were detected in the conditioned medium of ISt-1 cells. ISt-1 is considered to be an AFP-producing gastric cancer cell line and will provide a useful tool for the study of production and secretion of AFP from cells.
Terashima
M
M
First Department of Surgery, School of Medicine, Iwate Medical University.
Ikeda
K
K
Maesawa
C
C
Kawamura
H
H
Niitsu
Y
Y
Satoh
M
M
Saito
K
K
D003470:Culture Media; D006801:Humans; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000096:biosynthesis*
D003470:Culture Media; D000509:alpha-Fetoproteins