Minigene rescues acetylcholinesterase lethal mutations in Drosophila melanogaster.
The gene encoding acetylcholinesterase in Drosophila melanogaster is over 34,000 base-pairs long. We have constructed a 5800 base-pair minigene containing 1500 base-pairs of genomic sequence upstream from the transcription start spliced to the coding sequence, but lacking the nine introns. After germline genetic transformation, this minigene rescues acetylcholinesterase lethal mutants. Tissue-specific distribution appears normal. This allows us to test site-directed mutations of acetylcholinesterase. In a first effort, deletion of most of the unusual 1000 bases leader and its intriguing short open reading frames showed no effect on gene expression. The way is open to study in vivo the structure-function relationships of acetylcholinesterase and insecticide resistance.
Hoffmann
F
F
Department of Zoology and Animal Biology, University of Geneva, Switzerland.
Fournier
D
D
Spierer
P
P
D000110:Acetylcholinesterase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
D012333:RNA, Messenger; D000110:Acetylcholinesterase