Tumorigenesis of a v-Ha-ras-expressing pre-B cell line selects for c-myc activation.
Seven tumors independently derived from a v-Ha-ras-expressing pre-B cell line were examined to determine the oncogene activations cooperating with v-Ha-ras in in vivo tumor progression. The pre-B cell line was generated by infection with Moloney murine leukemia virus (MoMuLV) and a MoMuLV-derived recombinant expressing v-Ha-ras. Two of seven tumors possessed a MoMuLV integration immediately upstream and in reverse transcriptional orientation to c-myc. This correlated with a 3-fold increased level of c-myc mRNA. Two other tumors displayed elevated c-myc mRNA levels, although the mechanism of enhanced expression was unclear. Thus the tumor progression of a v-Ha-ras-expressing murine pre-B cell line selects for the activation of c-myc.
Chen
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SC
Department of Microbiology, Michigan State University, East Lansing 48824-1101.
Redenius
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D
Schwartz
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RC
D000818:Animals; D001402:B-Lymphocytes; D002460:Cell Line; D002472:Cell Transformation, Viral; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D005786:Gene Expression Regulation; D016259:Genes, myc; D011905:Genes, ras; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D012313:RNA; D004247:DNA