title: Identification of a conserved epitope in class I alcohol dehydrogenase isoenzymes using monoclonal antibodies.
abstract: Two monoclonal antibodies raised against native horse alcohol dehydrogenase (HADH) bind preferentially to the enzyme attached to solid supports and recognize the denatured and carboxymethylated HADH subunits. Both antibodies cross-react with the human class I isoenzymes but do not recognize the class III ADH isoenzyme. Protease digestion, electrophoresis and HPLC have been used to identify the linear epitope which is contained in the sequence Pro344-Glu357 of the HADH subunit.
authors.affiliation: International Institute of Genetics and Biophysics, CNR, Naples, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Adinolfi
authors.forename: N
authors.initials: N
authors.lastname: Leone
authors.forename: D
authors.initials: D
authors.lastname: Swallow
authors.forename: D A
authors.initials: DA
authors.lastname: Hopkinson
authors.forename: M C
authors.initials: MC
authors.lastname: Zapponi
authors.forename: G
authors.initials: G
authors.lastname: Ferri
authors.forename: L
authors.initials: L
authors.lastname: Camardella
authors.forename: S
authors.initials: S
authors.lastname: Ronchi
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000145:classification / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D000939:Epitopes / Q000737:chemistry; D006736:Horses; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000145:classification / Q000276:immunology*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007527:Isoenzymes; D000426:Alcohol Dehydrogenase