title: Alpha-fetoprotein uptake by differentiating neuroretinal structures of the chick embryo.
abstract: Internalization of exogenous fluoresceinated alpha-fetoprotein (FITC-AFP) was studied at different stages of development on embryonic chick neural retinas maintained, for short periods, in organ cultures. Cellular localization of endogenous, native AFP was carried out by immunohistoperoxidase methods. Cells which specifically internalized exogenous FITC-AFP (neurons and their processes) were precisely those showing positive immunolabeling for the endogenous, native protein. Such a result supports the hypothesis of a predominantly exogenous origin of intracellular neuroretinal AFP. A precise topography and temporal sequence of AFP labeling after internalization in retinal structures is given. AFP uptake was not displayed by undifferentiated cell precursors or germinal cell layers but was apparent in cells with phenotypic characteristics of maturing neurons. Nerve fibers and synaptic layers actively internalized FITC-AFP at specific stages of development. Fully differentiated neurons and processes did not internalize AFP. The possible role of AFP, a carrier of biologically active substances such as fatty acids, in neural retina differentiation is discussed.
authors.affiliation: Laboratoire de Chimie des Protéines, IRSC-CNRS, Villejuif, France.
authors.forename: M
authors.initials: M
authors.lastname: Hajeri-Germond
authors.forename: J
authors.initials: J
authors.lastname: Trojan
authors.forename: J
authors.initials: J
authors.lastname: Uriel
mesh_terms: D000818:Animals; D001692:Biological Transport; D002642:Chick Embryo; D002645:Chickens / Q000254:growth & development / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000378:metabolism; D009924:Organ Culture Techniques; D010786:Photoreceptor Cells / Q000378:metabolism; D012160:Retina / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000032:analysis / Q000493:pharmacokinetics*
chemical_list: D000509:alpha-Fetoproteins; D016650:Fluorescein-5-isothiocyanate