[The strain-specific diagnosis of influenza by using lanthanide immunofluorescence analysis based on monoclonal antibodies to the hemagglutinin of the influenza A virus].
Nine monoclonal antibodies (MCA) to hemagglutinin of influenza A/Taiwan/1/86 (H1N1) virus and 5 MCA to influenza A/Mississippi/1/85 (H3N2) virus were generated and characterized. The MCA were used for the development of diagnostic test systems on the basis of time-resolved fluoroimmunoassay. The same MCA were used as primary and detecting antibodies in the test system specific for HA of the H1 serosubtype, whereas in the test system specific for influenza A serosubtype H3 virus MCA of different epitope appurtenance were used as primary and secondary antibodies. The sensitivity of the test system for HA of serosubtype H1 was found to be 10 ng/ml and that for serosubtype H3 5 nh/ml. The developed test systems were tried on the clinical material collected during the epidemic periods of 1983-1989.
Busse
T L
TL
Khalling
T A
TA
Khristova
M L
ML
Allikmets
E U
EU
Kharitonenkov
I G
IG
Saarma
M J
MJ
Halonen
P
P
D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006389:Hemagglutinins, Viral / Q000276:immunology*; D006801:Humans; D009980:Influenza A virus / Q000145:classification / Q000276:immunology*; D007251:Influenza, Human / Q000175:diagnosis*; D008674:Metals, Rare Earth; D012680:Sensitivity and Specificity; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006389:Hemagglutinins, Viral; D008674:Metals, Rare Earth
Shtammospetsificheskaia diagnostika grippa s ispol'zovaniem lantanidnogo immunofliuorestsentnogo analiza na osnove monoklonal'nykh antitel k gemaggliutininu virusa grippa A.