Soluble and particulate inositol 1,4,5-trisphosphate 5-phosphatases show common antigenic determinants.
Inositol 1,4,5-trisphosphate 5-phosphatase catalyses the dephosphorylation of the phosphate in the 5-position from inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate. One particulate and two soluble enzymes were previously described in bovine brain. In this study, we have obtained a precipitating antiserum against soluble type I inositol 1,4,5-trisphosphate 5-phosphatase. The particulate, but not the soluble type II enzyme, was immunoprecipitated by the serum. Inositol 1,4,5-trisphosphate 5-phosphatase activity from crude extracts of rat brain, human platelets and rat liver were immunoprecipitated by the same antibodies, suggesting the existence of common antigenic determinant among inositol 1,4,5-trisphosphate 5-phosphatases of diverse sources.
Verjans
B
B
Institut de Recherche Interdisciplinaire (IRIBHN), Université Libre de Bruxelles, Brussels, Belgium.
Hollande
F
F
Moreau
C
C
Lejeune
C
C
Erneux
C
C
D000818:Animals; D001792:Blood Platelets / Q000201:enzymology; D001921:Brain / Q000201:enzymology; D002417:Cattle; D000939:Epitopes; D006801:Humans; D000072181:Inositol Polyphosphate 5-Phosphatases; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology; D010744:Phosphoric Monoester Hydrolases / Q000276:immunology* / Q000302:isolation & purification; D011233:Precipitin Tests; D051381:Rats; D012995:Solubility
D000939:Epitopes; D007527:Isoenzymes; D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases