Cloning and functional expression of human cDNA for the ETB endothelin receptor.
We report the cloning of human cDNA encoding an ETB (non-isopeptide-selective) subtype of the endothelin receptor. The predicted amino acid sequence of the human ETB endothelin receptor was 87.8% and 62.9% identical with the previously cloned rat ETB and ETA receptors, respectively. COS cells transiently transfected with the cloned cDNA expressed specific, high-affinity binding sites for endothelin isopeptides and responded to the peptides with a transient increase of [Ca2+]i; endothelin-1 and endothelin-3 exhibited approximately equal potencies both in displacing 125I-labeled endothelin-1 binding and in eliciting [Ca2+]i transients. The ETB receptor mRNAs were expressed in various human tissues and also in the intact porcine aortic intimal cells ex vivo.
Sakamoto
A
A
Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
Yanagisawa
M
M
Sakurai
T
T
Takuwa
Y
Y
Yanagisawa
H
H
Masaki
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D016232:Endothelins / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007583:Jejunum / Q000502:physiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D017466:Receptors, Endothelin; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
D016232:Endothelins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D011994:Recombinant Proteins; D011061:Poly A; D012313:RNA; D002118:Calcium