[3'-Mercapto-3'-deoxythymidine-5'-triphosphate as a terminator of DNA synthesis catalyzed by RNA-dependent DNA-polymerases].
3'-Mercapto-3'-deoxy-TTP was synthesized and tested as DNA chain terminator nucleotide for calf thymus alpha DNA polymerase, E. coli DNA polymerase I (Klenow fragment), terminal deoxyribonucleotidyltransferase (Bollum enzyme) and reverse transcriptase from AMV- and HIV-I-viruses. It was shown that the compound terminates DNA chain elongation by reverse transcriptases selectively and irreversibly. Other tested DNA polymerases do not use this nucleotide analogue as a substrate. 3'-Mercapto-3'-deoxythymidine was tested on lymphoblastoid T-cell line MT-4 with HIV-viruses and shown to suppress viruses as efficiently as 3'-azido-3'-deoxythymidine.
Iuzhakov
A A
AA
Chidzhavadze
Z G
ZG
Bibilashvili
P Sh
PSh
Kraevskiĭ
A A
AA
Galegov
G A
GA
Korneeva
M N
MN
Posik
D N
DN
Kilesso
T Iu
TIu
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001483:Base Sequence; D002384:Catalysis; D002417:Cattle; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects; D004256:DNA Polymerase I / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000201:enzymology; D015497:HIV-1 / Q000201:enzymology; D008969:Molecular Sequence Data; D018894:Reverse Transcriptase Inhibitors; D013942:Thymine Nucleotides / Q000494:pharmacology*
D018894:Reverse Transcriptase Inhibitors; D013942:Thymine Nucleotides; C070668:3'-mercapto-3'-deoxythymidine-5'-triphosphate; D004247:DNA; D004256:DNA Polymerase I
3'-Merkapto-3'-dezoksitimidin-5'-trifosfat kak terminator sinteza DNK, kataliziruemogo RNK-zavisimymi DNK-polimerazami.