Cadmium, selenium, and tellurium chelators in Aspergillus terreus.
Aspergillus terreus was cultivated on Harrold's medium supplemented with 0.1% (w/v) cadmium chloride as well as on sulfur free medium amended with 0.1% (w/v) sodium selenite and potassium tellurite separately. The cell free extract of the fungus for each treatment was fractionated on a column packed with Sephadex G 75. The results demonstrated the ability of the fungus to synthesize several cadmium, selenium, and tellurium-binding proteins as well as metallothionein. The results suggested the biosynthesis of heavy metals chelators as well. The amino acids composition of a cadmium-binding metallothionein revealed the presence of high levels of both aromatic and sulfur amino acids in the hydrolysate.
Abbass
I M
IM
Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt.
Razak
A A
AA
D000596:Amino Acids / Q000032:analysis; D001230:Aspergillus / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis; D002850:Chromatography, Gel; D005656:Fungal Proteins / Q000032:analysis; D008668:Metallothionein / Q000096:biosynthesis; D012643:Selenium / Q000378:metabolism*; D051137:Selenium-Binding Proteins; D013691:Tellurium / Q000378:metabolism*
D000596:Amino Acids; D002352:Carrier Proteins; D005656:Fungal Proteins; D051137:Selenium-Binding Proteins; C004203:cadmium-binding protein; D002104:Cadmium; D008668:Metallothionein; D012643:Selenium; D013691:Tellurium