[Immunohistologic investigations of paraffin-embedded lymph nodes after bone marrow transplantation in autopsy samples].
A histological and immunohistochemical analysis of paraffin-embedded lymph nodes from 31 autopsies after bone marrow transplantation (BMT) was performed (20 allogeneic, 10 autologous, 1 syngeneic). Monoclonal antibodies against CD 45RB, CD 20, CD 21, CD 35. CD 43, CD 45RO, CD 76 and Ki-B3, and antisera for detection of S 100-protein and immunoglobulin isotypes were used. None of the lymph nodes showed a regular reconstitution. The lymphoid cells, scattered in a diffuse pattern, were mainly CD 43-positive. Most of them also expressed the CD 45RO antigen. CD 20- and Ki-B3 positive lymphoid cells were nearly absent within the first 100 days after BMT. After that time B cell follicles were detectable in a few cases. Surprisingly, in nearly all cases with infectious complications, numerous plasma cells could be found. The origin of this plasma cells is discussed.
Friedrich
T
T
Pathologisches Institut, Universität Leipzig.
Kupper
H
H
Püschel
W
W
Pönisch
W
W
Helbig
W
W
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D001344:Autopsy; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D016026:Bone Marrow Transplantation / Q000276:immunology* / Q000473:pathology; D006652:Histological Techniques; D006801:Humans; D007106:Immune Sera; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology* / Q000473:pathology; D010232:Paraffin; D009418:S100 Proteins / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D007106:Immune Sera; D009418:S100 Proteins; D010232:Paraffin
Immunhistologische Untersuchungen an paraffineingebetteten Lymphknoten Verstorbener nach Knochenmarktransplantation.