Competitive dissociation of encephalitogenic complexes between antigen presenting cells and myelin basic protein.
Splenic T cells from myelin basic protein (MBP)-immunised Lewis rats were activated to transfer experimental autoimmune encephalomyelitis (EAE) by co-culture with MBP-pulsed lymphoid dendritic cells (DC). MBP-pulsed DC could be kept for at least 24 h at 37 degrees C in antigen-free medium without affecting their ability subsequently to activate encephalitogenic T cells. However, MBP-pulsed DC were rendered much less stimulatory after a 6 h, but not 2 h, secondary incubation with ovalbumin. Thus, although encephalitogenic complexes between MBP and DC appear very stable in the absence of competing antigens, in their presence, antigen exchange can take place over a period of a few hours; this has positive implications for therapy of EAE by antigen competition.
Gautam
A M
AM
Multiple Sclerosis Society Laboratory, Institute of Neurology, London, U.K.
Glynn
P
P
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D001327:Autoimmune Diseases / Q000276:immunology*; D001667:Binding, Competitive; D002450:Cell Communication; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D004676:Myelin Basic Protein / Q000276:immunology* / Q000378:metabolism; D010047:Ovalbumin / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000502:physiology
D004676:Myelin Basic Protein; D010047:Ovalbumin