Electron microscopic study of receptor mediated endocytosis of a monoclonal antibody (RoMo-1) against the surface marker CD 14 of human monocytes.
The internalization of the gold labelled monoclonal antibody RoMo-1 recognizing the CD 14 surface molecule of human monocytes was studied by electron microscopy. Monocyte enriched mononuclear cells (MNC) from the peripheral blood of healthy donors were incubated for 15, 30, 60, and 90 min with gold marked RoMo-1 in Eagle MEM at 37 degrees C. The process of internalization of RoMo-1 occurs as a receptor mediated endocytosis (RME), as described for other ligands.
Jonas
L
L
Electron Microscopic Centre, Wilhelm-Pieck-University of Rostock, GDR.
Schütt
C
C
Neels
P
P
Walzel
H
H
Siegl
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D015703:Antigens, CD / Q000276:immunology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D002462:Cell Membrane / Q000648:ultrastructure; D004705:Endocytosis; D011992:Endosomes / Q000502:physiology / Q000648:ultrastructure; D006046:Gold; D017979:Gold Colloid; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D018950:Lipopolysaccharide Receptors; D008247:Lysosomes / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008854:Microscopy, Electron; D012016:Reference Values; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D017979:Gold Colloid; D018950:Lipopolysaccharide Receptors; C058457:Aurodye; D006046:Gold