Expression of the hst-1 and c-kit protooncogenes in human testicular germ cell tumors.
Seventy testicular germ cell tumors were analyzed at the DNA and RNA levels for the c-kit, hst-1, and int-2 oncogenes using Northern and Southern blot analyses, respectively. There were significant differences in oncogene expression between seminomas and nonseminomas with c-kit being expressed in 24 of 30 (80%) seminomas but in only 3 of 40 (7%) nonseminomatous tumors (P = 0.0001, chi 2 test) and hst-1 being expressed in 24 of 38 (63%) nonseminomas but only 1 of 24 (4%) of seminomas (P = 0.0001, chi 2 test), demonstrating an inverse relationship in the expression pattern of these 2 oncogenes in human testicular germ cell tumors. A significant association between tumor stage and hst-1 expression in the nonseminoma group was found (P = 0.0002, chi 2 test). No gross alterations in the c-kit, hst-1, and int-2 loci were found at the DNA level and no int-2 mRNA expression was detected in any of the germ cell tumors examined.
Strohmeyer
T
T
Department of Medicine, University of California, School of Medicine, Los Angeles 90024.
Peter
S
S
Hartmann
M
M
Munemitsu
S
S
Ackermann
R
R
Ullrich
A
A
Slamon
D J
DJ
D015139:Blotting, Southern; D004273:DNA, Neoplasm / Q000032:analysis; D051519:Fibroblast Growth Factor 4; D005346:Fibroblast Growth Factors / Q000032:analysis*; D005784:Gene Amplification; D006133:Growth Substances / Q000032:analysis*; D006801:Humans; D008297:Male; D009373:Neoplasms, Germ Cell and Embryonal / Q000235:genetics*; D015513:Oncogene Proteins / Q000032:analysis*; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D019009:Proto-Oncogene Proteins c-kit; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D013736:Testicular Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm; C496445:FGF4 protein, human; D051519:Fibroblast Growth Factor 4; D006133:Growth Substances; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D005346:Fibroblast Growth Factors; D019009:Proto-Oncogene Proteins c-kit