[Fast direct method of obtaining metaphase and prometaphase chromosomes from chorion biopsy cells and human embryos during the lst semester of pregnancy].
Samples of chorionic villi and embryonic tissues (brain, brain--sheaths) are thoroughly washed with Hank's solution, immediately subjected to hypotonic treatment (0.9% sodium citrate plus few drops of 0.01% colchicine) 37 degrees C, 30 min, prefixed 20 min with equal amount of standard fixative mixture, twice fixed in standard fixative solution (1 hour, -10 degrees C), hydrated with equal volume of distilled water (5-10 min), dried, macerated directly on the slide with 60% acetic acid. The cell suspension is then evenly spread on the slide surface, dried, postfixed and stained. The method provides sufficient amount of metaphase and prometaphase mitotic plates suitable for differentiating staining in 1.5-2 hours after sampling and might be recommended for routine chromosomal analysis in prenatal diagnosis of inherited diseases during early pregnancy.
Baranov
V S
VS
Lebedev
V M
VM
Poleev
A V
AV
Mikhaĭlova
E P
EP
Rybalko
A V
AV
Shved
N V
NV
D001706:Biopsy; D002824:Chorionic Villi; D002877:Chromosomes, Human; D003584:Cytological Techniques; D004622:Embryo, Mammalian; D005260:Female; D005865:Gestational Age; D006801:Humans; D008677:Metaphase; D011247:Pregnancy; D011296:Prenatal Diagnosis; D013194:Staining and Labeling
Uskorennyĭ priamoĭ metod polucheniia metafaznykh i prometafaznykh khromosom iz kletok bioptata khoriona i émbrionov cheloveka v pervom trimestre beremennosti.