Inter-species complementation of a rosy deficiency in Drosophila melanogaster.
A chimeric Xdh gene was constructed in vitro, by recombining DNA sequences from the Dipterans Drosophila melanogaster and Calliphora vicina. The ry506 strain, an eye-colour mutant of Drosophila that is deficient for Xdh, was genetically transformed with the recombinant gene. Transformed flies with ry+ eye phenotype and increased resistance to purine were obtained, showing that the chimeric XDH is physiologically active in Drosophila. XDH activity was detected in crude extracts from transformed flies, yet at lower levels than in wild-type controls. The amounts of Xdh transcripts in the transformants were found to be 8 to 16% of the amount of wild-type ry mRNA, suggesting that Calliphora Xdh sequences may be relatively inefficient for mRNA production in Drosophila, or may produce unstable mRNA.
Tiveron
M C
MC
Laboratoire de Biologie Cellulaire et Moléculaire du C.N.R.S., Marseille, France.
Houde
M
M
Vola
C
C
Hipeau-Jacquotte
R
R
Berreur
P
P
Brégégère
F
F
D000818:Animals; D015152:Blotting, Northern; D002678:Chimera; D004331:Drosophila melanogaster / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012313:RNA / Q000032:analysis; D011995:Recombination, Genetic; D013045:Species Specificity; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D012313:RNA