title: Role of ribosome release in the basal level of expression of the Escherichia coli gene pheA.
abstract: In Escherichia coli, the expression of the phenylalanine biosynthetic operon pheA is regulated by an attenuation mechanism. In the presence of excess phenylalanine, gene expression was decreased to 10% of the fully deattenuated level. To understand the factors that determine the basal level of pheA expression, we examined the role of ribosome release from the leader peptide stop codon UGA. The transcriptional readthrough from the pheA attenuator decreased by over 2-fold in the presence of the defective release factor 2. However, a release factor 1 (UAG and UAA specific) mutation did not influence the basal level of pheA expression. These results support the proposal that the release of translating ribosomes from the leader peptide stop codon in stem 2 of the pheA attenuator plays a crucial role in determining the basal level of expression of this gene.
authors.affiliation: Department of Molecular Biology and Biochemistry, School of Biological Sciences, University of California, Irvine 92717.
authors.forename: N
authors.initials: N
authors.lastname: Gavini
authors.forename: L
authors.initials: L
authors.lastname: Pulakat
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D010443:Peptide Chain Termination, Translational; D010649:Phenylalanine / Q000096:biosynthesis* / Q000235:genetics; D012329:RNA, Bacterial; D012270:Ribosomes / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D010649:Phenylalanine