Enhanced insulin-like growth factor I gene expression in regenerating rat pancreas.
Insulin-like growth factor I (IGF-I) mRNA expression was studied after 90% partial pancreatectomy in the rat to determine whether IGF-I was associated with pancreatic regeneration. The level of IGF-I mRNA was maximally increased (4-fold above control value) 3 days after pancreatectomy, but thereafter gradually decreased, returning to control levels by 14 days after surgery. By in situ hybridization, IGF-I mRNA in both pancreatectomized and sham-operated rats was localized to capillary endothelial cells, indicating that this is the site of IGF-I expression in the normal rat pancreas. However, enhanced IGF-I mRNA expression was localized to focal areas of regeneration unique to pancreatectomized rats. In these areas, epithelial cells of proliferating ductules and individual connective tissue cells expressed IGF-I, suggesting that IGF-I may play an important role in the growth or differentiation of pancreatic tissue.
Smith
F E
FE
E. P. Joslin Research Laboratory, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215.
Rosen
K M
KM
Villa-Komaroff
L
L
Weir
G C
GC
Bonner-Weir
S
S
D000818:Animals; D001345:Autoradiography; D015342:DNA Probes; D015870:Gene Expression; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D007700:Kinetics; D008297:Male; D009693:Nucleic Acid Hybridization; D010179:Pancreas / Q000502:physiology*; D010180:Pancreatectomy; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012038:Regeneration; D013462:Sulfur Radioisotopes; D013997:Time Factors
D015342:DNA Probes; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D012313:RNA; D007334:Insulin-Like Growth Factor I