Studies on linear epitopes of Semliki Forest virus in protection studies against lethal challenge virus infection.
Purified reduced and non-reduced glycoproteins E1 and E2 of Semliki Forest Virus (SFV) were used to investigate the protection potency to prevent clinical disease after lethal virus challenge. In parallel synthetic oligopeptides deduced from conserved regions of the nucleotide sequences coding for the glycoproteins E1 and E2 were included. It could be demonstrated that both reduced and non-reduced glycoprotein preparations induced protection against lethal virus challenge, whereas the oligopeptides did not. The role of linear epitopes in immunity and their potential use as synthetic vaccines against Alphaviruses are critically discussed.
Truyen
U
U
Institute for Virology, Hannover Veterinary School, F. R. G.
Kaaden
O R
OR
D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012376:Rodent Diseases / Q000517:prevention & control*; D012672:Semliki forest virus / Q000276:immunology*; D014036:Togaviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
D000939:Epitopes; D014759:Viral Envelope Proteins