Changes in beta-tubulin isoforms and their RNA level in synchronized tobacco cells.
Tobacco BY-2 cells were synchronized by an aphidicolin treatment, and their beta-tubulin isoforms and their mRNA were analyzed by Western, Northern and dot blottings. The relative ratio of the beta-tubulin isoforms changed with the progress of cell cycle stage. By Northern blot hybridization of poly(A)+RNAs with a cloned carrot beta-tubulin cDNA probe, a single band of about 1.6 kb was detected throughout the cell cycle. Dot blot hybridization showed that beta-tubulin mRNA existed in all stages in the cell cycle at a relatively constant level, though it accumulated slightly more than average at M phase and decreased during G1 phase.
Nishimura
M
M
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Tanigaki
C
C
Okamura
S
S
D016590:Aphidicolin; D002478:Cells, Cultured; D015151:Immunoblotting; D008940:Mitotic Index / Q000502:physiology; D010947:Plants, Toxic; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism*
D014404:Tubulin; D016590:Aphidicolin; D012313:RNA