title: In vivo inhibition of TonB-dependent processes by a TonB box consensus pentapeptide.
abstract: The TonB box, a conserved pentapeptide sequence found in TonB-dependent colicins and receptors, is thought to interact physically with the TonB protein to facilitate TonB-dependent processes. Strains of Escherichia coli were treated in vivo with the synthetic TonB box pentapeptide Glu-Thr-Val-Ile-Val. The pentapeptide inhibited several TonB-dependent processes, including cell growth in low-iron medium, phi 80 infection, and killing by colicins B and Ia. Two unrelated control pentapeptides had no effect on TonB-dependent processes.
authors.affiliation: Department of Microbial Genetics and Biochemistry, American Cyanamid Company, Lederle Laboratories, Pearl River, New York 10965.
authors.forename: M
authors.initials: M
authors.lastname: Tuckman
authors.forename: M S
authors.initials: MS
authors.lastname: Osburne
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport / Q000502:physiology; D003087:Colicins / Q000378:metabolism; D003090:Coliphages; D016384:Consensus Sequence; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D029968:Escherichia coli Proteins; D007501:Iron / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D003087:Colicins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D009842:Oligopeptides; C057616:tonB protein, Bacteria; C489181:tonB protein, E coli; D007501:Iron