[Comparison of flow cytometric, static cytometry and tumor cytogenetic investigation results human renal cell carcinomas].
Published data about the frequency of DNA-aneuploidy of renal cell tumours show a great variation. The own study was performed to clarify whether this fact is due to methodological discrepancies or to intratumoural heterogeneity of DNA ploidy. The comparison of DNA ploidy assessed by flow cytometry (fresh and paraffin-embedded tissue) and static cytometry (paraffin-embedded tissue) in equivalent tumour regions showed identical results. 71% of clear cell renal carcinomas showed intratumoural heterogeneity of DNA ploidy. In 55% there was either a combination of diploid and aneuploid stem lines and/or a combination of different aneuploid stem lines ("heterogenous aneuploidy"). The rate of aneuploidy was found to be higher with increasing cytoplasmic eosinophilia of the clear cell renal carcinoma cells. Studies which address the prognostic significance of DNA ploidy of renal carcinomas should specifically investigate on the significance of heterogenous aneuploidy for prognosis.
Krech
R H
RH
Institut für Pathologie, Medizinischen Universität zu Lübeck.
Baretton
G
G
Fonatsch
C
C
Diebold
J
J
Früchtnicht
W
W
Löhrs
U
U
D000782:Aneuploidy; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000473:pathology*; D003581:Cytodiagnosis / Q000379:methods; D003582:Cytogenetics / Q000379:methods; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000473:pathology*; D011003:Ploidies
D004273:DNA, Neoplasm
Vergleich durchflusszytophotometrischer, einzelzellzytophotometrischer und tumorzytogenetischer Untersuchungsergebnisse an Nierenzellkarzinomen des Menschen.