Transport of 4-hydroxyphenylacetic acid in Klebsiella pneumoniae.
Klebsiella pneumoniae M5a1 has been shown to possess an inducible transport system for 4-hydroxyphenylacetate (4-HPA). This transport system has a Kt of 16.3 microM and a maximal velocity of 31.2 nmol/min (milligrams dry weight). The transport system has been inhibited by inhibitors of energy metabolism with a concomitant decrease in cellular ATP concentrations, and the 4-HPA binding activity has been detected in the crude shock extracts. All these observations indicate that 4-HPA uptake is an active transport which involves a periplasmic binding protein and it seems to be energized by phosphate bond energy.
Allende
J L
JL
Departamento de Bioquímica y Biologiá Molecular IV, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Gibello
A
A
Martin
M
M
Garrido-Pertierra
A
A
D000255:Adenosine Triphosphate / Q000378:metabolism; D001692:Biological Transport; D002250:Carbon Radioisotopes; D004734:Energy Metabolism; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism*; D011865:Radioisotope Dilution Technique
D002250:Carbon Radioisotopes; D010648:Phenylacetates; C008070:4-hydroxyphenylacetic acid; D000255:Adenosine Triphosphate