Kainate activated single channel currents as revealed by domoic acid.
We have studied the properties of kainic acid receptor-activated channels using domoic acid as an agonist. Similarities of the electrophysiological, pharmacological and noise properties of domoic acid and kainic acid-evoked currents confirm that domoate is a potent and specific agonist of the kainate receptor. Single-channel properties of domoic acid-evoked currents were directly determined from outside-out membrane patches for the first time, and results were compared with those obtained by fluctuation analysis of macroscopic currents. Small conductance cationic-selective channels of approximately 4 pS and a mean open time of 2 to 3 ms were detected using both methods.
Sciancalepore
M
M
Scuola Internazionale Superiore di Studi Avanzati, Trieste, Italy.
Gałdzicki
Z
Z
Zheng
X
X
Moran
O
O
D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000502:physiology; D004594:Electrophysiology; D007473:Ion Channels / Q000187:drug effects*; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D008274:Magnesium / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009467:Neuromuscular Depolarizing Agents / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D018092:Kainic Acid Receptors; D017981:Receptors, Neurotransmitter / Q000187:drug effects
D007473:Ion Channels; D007608:Kainic Acid; D008274:Magnesium; D009467:Neuromuscular Depolarizing Agents; D018092:Kainic Acid Receptors; D017981:Receptors, Neurotransmitter; C012301:domoic acid