title: Immunoreactive endothelin in urine of patients with and without diabetes mellitus.
abstract: Immunoreactive (ir)-endothelin (ET) in urine was studied with a radioimmunoassay in patients with diabetes mellitus (DM) and non-DM diseases including endocrine disorders, primary glomerular diseases, autoimmune diseases, and hematological malignancies. Twenty-four-hour excretions (mean +/- SEM) of ir-ET were 8.0 +/- 0.9 pmol/day in the DM group (n = 13) and 9.5 +/- 1.2 pmol/day in the non-DM group (n = 51). No significant differences among DM and other disease groups were noted with respect to 24-h ir-ET excretion. Reverse-phase high-performance liquid chromatography of a normal urine extract revealed a major peak eluting later than ET-1. Gel chromatography revealed a single major peak in a smaller molecular weight (MW) region in normal urine and an additional peak in larger MW region in a urine extract from a DM patient. Urinary ir-ET consists of at least two components which may be metabolites of ET or ET precursors in plasma or peptides derived from the kidney.
authors.affiliation: Second Department of Internal Medicine, Tohoku University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Totsune
authors.forename: M
authors.initials: M
authors.lastname: Sone
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Ohneda
authors.forename: K
authors.initials: K
authors.lastname: Itoi
authors.forename: O
authors.initials: O
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Mouri
authors.forename: K
authors.initials: K
authors.lastname: Yoshinaga
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000652:urine*; D016232:Endothelins / Q000276:immunology / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay
chemical_list: D016232:Endothelins