A novel compound, depudecin, induces production of transformation to the flat phenotype of NIH3T3 cells transformed by ras-oncogene.
A novel compound, depudecin, induced production of the flat phenotype of Ki-ras-transformed NIH3T3 cells at the low concentration of 1 microgram/ml. This effect was reversible. Actin stress fiber was detected in these cells after depudecin treatment. Almost complete reversion to the flat phenotype was observed at 6 h after depudecin addition. The synthesis of ras-mRNA did not decrease enough with depudecin treatment at the concentration of 10 micrograms/ml to reverse the transformed morphology.
Sugita
K
K
Department of Microbiology, Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Yoshida
H
H
Matsumoto
M
M
Matsutani
S
S
D016475:3T3 Cells; D000199:Actins / Q000187:drug effects / Q000378:metabolism; D000466:Alkadienes / Q000494:pharmacology*; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004852:Epoxy Compounds / Q000494:pharmacology*; D005233:Fatty Alcohols / Q000494:pharmacology*; D015870:Gene Expression; D011905:Genes, ras; D006877:Hydroxamic Acids / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics
D000199:Actins; D000466:Alkadienes; D000935:Antifungal Agents; D004852:Epoxy Compounds; D005233:Fatty Alcohols; D006877:Hydroxamic Acids; D012333:RNA, Messenger; C072251:depudecin; C012589:trichostatin A