Analysis of integrin mRNA in human and rodent tumor cells.
Several human and rodent tumor cell lines were examined for the presence of integrin mRNAs by dot- and Northern-blot analysis. All tumor cells tested expressed mRNAs for alpha 5, alpha IIb, beta 1 and beta 3. The mRNA of beta 2 integrin was not detectable and that of alpha V integrin was found only in certain cells. Northern blotting was carried out in three selected tumor cell lines: Clone A, HEL and B16a. An apparent difference in the mRNA species coding for the alpha IIb beta 3 integrin, but not for alpha 5 and beta 1, was found. Our result suggests that alternative splicing of integrin genes may be one of the important mechanisms in regulating alpha IIb beta 3 expression and function in different tumor cells.
Chang
S Y
SY
Center for Molecular Biology, Wayne State University, Detroit, MI 48202.
Chen
Y Q
YQ
Fitzgerald
L A
LA
Honn
K V
KV
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D006801:Humans; D015151:Immunoblotting; D016023:Integrins / Q000235:genetics*; D051379:Mice; D008970:Molecular Weight; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats
D016023:Integrins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA