Catecholaminergic and peptidergic nerve components of intramural ganglia in the rat heart. An immunohistochemical study.
Immunohistochemical properties of the terminal nerve network in the rat heart were assessed by use of the elution-restaining method. The colocalization of the enzymes involved in catecholamine synthesis (tyrosine hydroxylase--TH. dopamine-beta-hydroxylase--DBH) as well as the respective distributions of the neuropeptides associated with the adrenergic nervous system (neuropeptide tyrosine--NPY, C-terminal flanking peptide of neuropeptide Y--C-PON) were studied in series of serial sections throughout the interatrial septum and the atrioventricular junction. Our data suggest that ganglion cells of sulcus terminalis as well as the epicardial ganglia enclosed between the superior vena cava and ascending aorta are VIP- and TH-negative, but neuropeptide Y- and DBH-immunoreactive. They give rise to three intraseptal nerves directed towards the specialised structures of the atrioventricular junction. These nerve fascicles contain abundant, thick TH-immunoreactive nerve fibres and scarce, thin NPY- and DBH-immunoreactive fibres. The cell bodies of the intramural ganglion cells localized between the right and left branches of the bundle of His (Moravec and Moravec 1984) are strongly TH- and DBH-immunoreactive. They are innervated by thick nerve fibres having the same immunohistochemical properties (NPY- and DBH-immunoreactivities) as those of a subpopulation of the epicardial ganglion cells and seem to supply some of the TH-immunoreactive nerve fibres directed via the intraseptal nerves to the epicardial ganglia. The existence of a multicomponent nerve network, characterized by a reciprocal innervation of the sinus node and atrioventricular node areas, is suggested by our immunohistochemical data.
Moravec
M
M
Laboratoire d'Energétique et de Cardiologie Cellulaire, INSERM, Faculté de Pharmacie, Dijon, France.
Moravec
J
J
Forsgren
S
S
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002395:Catecholamines / Q000502:physiology*; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D004745:Enkephalins / Q000378:metabolism; D005724:Ganglia / Q000166:cytology / Q000378:metabolism*; D006321:Heart / Q000294:innervation*; D006329:Heart Conduction System / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009412:Nerve Fibers / Q000378:metabolism / Q000648:ultrastructure; D009478:Neuropeptide Y / Q000378:metabolism; D010455:Peptides / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D002395:Catecholamines; D004745:Enkephalins; D009478:Neuropeptide Y; D010455:Peptides; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D015740:Calcitonin Gene-Related Peptide