Shiverer jimpy double mutant mice. V. Correlation of genotype and myelin proteins.
We have reexamined the levels of myelin basic protein (MBP) and proteolipid protein (PLP) in the brains of mice bred to carry both the shi/shi and jp/Y hypomyelination defects. The genotype of each putative double mutant was confirmed by direct DNA analysis: shi/shi by Southern blot analysis, and jp/Y by restriction enzyme analysis of polymerase chain reaction-amplified fragments. MBP and PLP levels were assessed by immunoblotting. All putative double mutants were found to be shi/shi. However, examination of the PLP locus revealed both jp and wild-type genotypes, the latter produced by an expected crossover. Animals proven to be shi/shi*jp/Y had no detectable MBP or PLP; those proven to be shi/shi*+/Y (the crossover) had no MBP but had PLP. These results differ from an earlier report of both MBP and PLP in the brains of presumed shi*jp animals.
Sinclair
A
A
Department of Neurology, Childrens Hospital, Boston, Mass.
Raz
Y
Y
Kirschner
D A
DA
Villa-Komaroff
L
L
Wolf
M K
MK
Billings-Gagliardi
S
S
D000818:Animals; D015139:Blotting, Southern; D001923:Brain Chemistry; D003434:Crossing Over, Genetic; D004252:DNA Mutational Analysis; D005838:Genotype; D051379:Mice; D008816:Mice, Jimpy / Q000235:genetics* / Q000378:metabolism; D008818:Mice, Neurologic Mutants / Q000235:genetics* / Q000378:metabolism; D004676:Myelin Basic Protein / Q000172:deficiency* / Q000235:genetics; D009185:Myelin Proteins / Q000032:analysis* / Q000235:genetics; D018991:Myelin Proteolipid Protein; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein