Digoxigenin as a probe label for in situ hybridization on skeletal tissues.
Non-specific staining was encountered using digoxigenin-labelled cDNA probes for in situ hybridization on sections of skeletal tissues. This staining was most pronounced in cartilaginous matrices. Experimental procedures indicate that the background staining is caused by antibody-binding to hydrophobic sites in the tissues revealed by proteolytic permeabilization. A protocol for minimizing this background is described.
Bland
Y S
YS
Department of Anatomy, St George's Hospital Medical School, Tooting, London, UK.
Critchlow
M A
MA
Ashhurst
D E
DE
D000818:Animals; D002356:Cartilage / Q000737:chemistry*; D004076:Digoxigenin / Q000737:chemistry* / Q000276:immunology; D005189:False Positive Reactions; D005333:Fetus / Q000033:anatomy & histology; D016591:Histocytological Preparation Techniques; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes / Q000737:chemistry*; D011092:Polyethylene Glycols; D011817:Rabbits; D013194:Staining and Labeling
D007202:Indicators and Reagents; D015341:Nucleic Acid Probes; D011092:Polyethylene Glycols; D004076:Digoxigenin