IL-1 receptor antagonist regulation of acute phase protein synthesis in human hepatoma cells.
The hepatoma cell line HuH-7 has recently been shown to synthesize serum amyloid A (SAA) in response to IL-1. IL-1 receptor antagonist (IL-1Ra) was able to completely inhibit the response of SAA to IL-1 but not the increase seen in response to IL-6. IL-1Ra was equally effective at inhibiting IL-1 alpha or IL-1 beta. At a 10-fold molar excess of IL-1Ra over IL-1 there was complete inhibition of the SAA response. Removal of IL-1 at 24 h rapidly reduced the SAA secreted over the next 24 h. Addition of IL-1Ra to the cells at this time was as effective as removal of IL-1 at inhibiting the subsequent secretion of SAA. IL-1Ra was less effective at inhibition of IL-1-induced haptoglobin secretion. We would conclude that IL-1Ra may play an important role in the regulation of acute phase protein synthesis.
Bevan
S
S
Molecular Immunology Unit, London School of Hygiene and Tropical Medicine, UK.
Raynes
J G
JG
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D006242:Haptoglobins / Q000096:biosynthesis; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000494:pharmacology; D008113:Liver Neoplasms / Q000378:metabolism*; D011506:Proteins / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D012709:Serum Albumin / Q000096:biosynthesis; D000685:Serum Amyloid A Protein / Q000096:biosynthesis*; D012795:Sialoglycoproteins; D014407:Tumor Cells, Cultured / Q000378:metabolism
D000209:Acute-Phase Proteins; D006242:Haptoglobins; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D011506:Proteins; D011994:Recombinant Proteins; D012709:Serum Albumin; D000685:Serum Amyloid A Protein; D012795:Sialoglycoproteins