Facile cleavage of RNA via phosphodiester linkage fission by Co(III) complex.
Adenylyl (3'-5')adenosine (ApA) is effectively cleaved to two adenosine molecules by [Co(trien)(H2O)2]3+ complex (trien: triethylenetetramine). The complex (0.20 M) accelerates the cleavage by 10(5) fold, decreasing half-life of ApA from 4000 years to 9.3 days. The reaction involves general base catalysis by the hydroxide ion bound to the Co(III) ion for the formation of adenosine 2',3'-cyclic phosphate (A greater than p), followed by the prompt cleavage of the intermediate to adenosine.
Matsumoto
Y
Y
Institute of Materials Science, University of Tsukuba, Ibaraki, Japan.
Yonezawa
K
K
Komiyama
M
M
D015226:Dinucleoside Phosphates / Q000378:metabolism*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000494:pharmacology*; D012313:RNA / Q000187:drug effects* / Q000378:metabolism; D014266:Trientine / Q000031:analogs & derivatives* / Q000494:pharmacology
D015226:Dinucleoside Phosphates; D009942:Organometallic Compounds; C070281:cobalt triethylene tetramine complex; C006412:adenylyl-(3'-5')-adenosine; D012313:RNA; D014266:Trientine