Rapid technique for the detection of carcinogens using mice liver microsomes.
Mice liver microsomes were prepared at low g (10,000 g) force by Ca(2+)-aggregation method and were used for the detection of carcinogens by degranulation technique. RNA/protein ratio of these microsomes was 0.177 which was comparable to rat liver microsomes. Per cent degranulation with two carcinogens (O-dianisidine and benzidine) at 20 micrograms concentration was 21 per cent on the basis of RNA/protein ratio basis with both the carcinogens. At 40 micrograms concentration the per cent degranulation was observed to be almost double. Both the carcinogens required NADPH for their activation.
Jagota
S K
SK
Department of Biochemistry, Panjab University, Chandigarh.
D000818:Animals; D015197:Carcinogenicity Tests / Q000379:methods*; D015550:Cell Degranulation / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects*; D009249:NADP / Q000494:pharmacology; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis
D011506:Proteins; D009249:NADP; D012313:RNA