[The use of detergents in the polarographic determination of superoxide dismutase and catalase activities].
A number of detergents were screened to choose the most suitable one for polarographic measurement of superoxide dismutase and catalase activity. Triton X-100 and triton X-305 nonionic detergents proved to be the best of all the tested ionic and nonionic detergents. The sensitivity of the method with these tritons is close to the sensitivity of the original method and, in contrast to it, does not involve addition of blood plasma. Effects of a number of substances, used in studies of biological samples, on activity measurements by the developed methods were under study. Superoxide dismutase activities were measured in tissues of hepatomas with different growth rates.
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D000818:Animals; D002374:Catalase / Q000378:metabolism*; D003902:Detergents; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D051379:Mice; D008809:Mice, Inbred C3H; D011048:Polarography / Q000379:methods; D013482:Superoxide Dismutase / Q000378:metabolism*
D003902:Detergents; D002374:Catalase; D013482:Superoxide Dismutase
Ispol'zovanie detergentov pri poliarograficheskom opredelenii aktivnosti superoksiddismutazy i katalazy.