Association of the HNK-1 epitope with 5'-nucleotidase from Torpedo marmorata (electric ray) electric organ.
5'-Nucleotidase isolated from the electric organ of the electric ray (Torpedo marmorata) has a molecular mass of 62 kDa and, on two-dimensional electrophoresis, separates into up to 13 isoforms within a pI range of 5.9-6.7. The N-terminal sequence data show a 71% identity over 17 amino acids with that previously published for the rat liver enzyme. All forms of 5'-nucleotidase are recognized by the HNK-1 monoclonal antibody. HNK-1 immunoreactivity is found at the surface of the Schwann-cell processes covering the synaptic terminals and in this respect corresponds to that of 5'-nucleotidase in the same tissue. Since a number of glycoproteins involved in cell recognition and cell adhesion carry the HNK-1 epitope, 5'-nucleotidase may play a role in cell-cell or cell-extracellular matrix interaction in addition to its activity as an enzyme.
Vogel
M
M
AK Neurochemie, Zoologisches Institut der J.W. Goethe-Universität, Frankfurt am Main, Federal Republic of Germany.
Kowalewski
H J
HJ
Zimmermann
H
H
Janetzko
A
A
Margolis
R U
RU
Wollny
H E
HE
D015720:5'-Nucleotidase / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis*; D015153:Blotting, Western; D018959:CD57 Antigens; D004557:Electric Organ / Q000201:enzymology*; D007124:Immunoenzyme Techniques; D007526:Isoelectric Point; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014101:Torpedo
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018959:CD57 Antigens; D015720:5'-Nucleotidase