[Reverse transcriptase of the human immunodeficiency virus: cloning, expression in Escherichia coli, purification of the enzyme, and production of monoclonal antibodies].
To express HIV-1 reverse transcriptase in E. coli a number of genetic constructions containing reverse transcriptase and virus protease nucleotide sequences was obtained. The products of expression were characterized; monoclonal antibodies to reverse transcriptase were produced. The purification of reverse transcriptase was carried out. The substantial proteolysis of reverse transcriptase during purification was shown. The purified preparation is predominantly, an active protein with Mr 57 kDa. Some properties of this protein differed from the reverse transcriptase isolated from HIV.
Rechinskiĭ
V O
VO
Barbashov
S F
SF
Degtiarev
I L
IL
Vorob'ev
S M
SM
Liakhov
D L
DL
Kostiuk
D A
DA
Starov
A I
AI
Matsevich
G R
GR
Kochetkov
S N
SN
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D002853:Chromatography, Liquid; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology*; D006868:Hydrolysis; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D010957:Plasmids; D012194:RNA-Directed DNA Polymerase / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D015183:Restriction Mapping; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase
Obratnaia transkriptaza virusa immunodefitsita cheloveka: klonirovanie, ékspressiia v Escherichia coli, ochistka fermenta i poluchenie monoklonal'nykh antitel.