[Immunohistologic photometric quantification of pyruvate kinase content of rat tumors].
86 benign and malignant tumours were investigated histologically and immunohistologically. Staining intensity was measured by scanning-photometry. The computed optical density was digitalized and evaluated with an image analysis system. The results indicate a significantly higher amount of M2-PK in malignant tumours. In the adenocarcinomas of the mammary gland, the content of the M2-PK increased parallel with malignancy (p less than 0.01; r = 0.743). The M2-PK can serve as an indicator of the grade of malignancy in mammary tumours of the rat.
Bahnemann
R
R
Abteilung Pathologie, BASF AG, Ludwigshafen.
Domingo
M
M
Eigenbrodt
E
E
Reinacher
M
M
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis*; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology / Q000473:pathology; D009367:Neoplasm Staging; D009369:Neoplasms / Q000201:enzymology* / Q000473:pathology; D009374:Neoplasms, Experimental / Q000201:enzymology* / Q000473:pathology; D011770:Pyruvate Kinase / Q000032:analysis*; D051381:Rats; D013053:Spectrophotometry / Q000379:methods
D014408:Biomarkers, Tumor; D007527:Isoenzymes; D011770:Pyruvate Kinase
Immunhistologische photometrische Quantifizierung des Pyruvatkinase-Gehaltes in Rattentumoren zur Dignitätsbestimmung.