Chromosomal localization of the gene for AA-type platelet-derived growth factor receptor (PDGFRA) in humans and mice.
The full-length cDNA of the receptor for human AA-type platelet-derived growth factor (PDGF) was used to assign the PDGFRA gene to region q11----q21 of human chromosome 4 and to mouse Chromosome 5 by somatic cell hybrid analysis. Since the same region also contains the c-kit oncogene homolog KIT, we carried out pulsed-field gel electrophoresis to determine the physical distance between the two genes in human DNA. The two probes, when successively applied to the same filters, hybridized to a 450-kb EagI-fragment but not to other common restriction fragments. The genes are separated by at least one NotI, one XhoI, and one SalI site.
Hsieh
C L
CL
Department of Genetics, Stanford University Medical Center, CA 94305-5428.
Navankasattusas
S
S
Escobedo
J A
JA
Williams
L T
LT
Francke
U
U
D000818:Animals; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D015342:DNA Probes; D005796:Genes; D006801:Humans; D051379:Mice / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019009:Proto-Oncogene Proteins c-kit; D011956:Receptors, Cell Surface / Q000235:genetics*; D017479:Receptors, Platelet-Derived Growth Factor; D015183:Restriction Mapping
D015342:DNA Probes; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D019009:Proto-Oncogene Proteins c-kit; D017479:Receptors, Platelet-Derived Growth Factor