title: Stage-specific expression of plasmodial proteins containing an antigenic marker of the intraerythrocytic cisternae.
abstract: A monoclonal antibody, LWLI, recognized 3 proteins of 45, 50 and 102 kDa in Plasmodium falciparum-infected erythrocytes. The 45- and 50-kDa proteins were parasite-encoded and displayed markedly different peptide maps, indicating that they were distinct plasmodial polypeptides with a common antigenic epitope rather than differentially processed forms of a primary translational product. The 45-kDa protein was present throughout intraerythrocytic growth, while the 50-kDa molecule was not detected earlier than 11 h in the life cycle. The 102-kDa protein was only expressed in trophozoite- and schizont-infected red cells: its structural relationship to the 45- and 50-kDa proteins, if any, remains undefined. By indirect immunofluorescence and immunoelectron microscopy, LWLI bound to flattened intraerythrocytic cisternae exported into the erythrocyte cytoplasm. The results support the theory that proteins recognized by the antibody were concentrated in these compartments and their common antigenic epitope may serve as a marker for the cisternae. Stage-specific expression of LWLI reactive proteins implicates developmental regulation of cisternal functions during asexual parasite development.
authors.affiliation: Dept. of Microbiology and Immunology, Stanford University, CA 94305.
authors.forename: W L
authors.initials: WL
authors.lastname: Li
authors.forename: A
authors.initials: A
authors.lastname: Das
authors.forename: J Y
authors.initials: JY
authors.lastname: Song
authors.forename: J L
authors.initials: JL
authors.lastname: Crary
authors.forename: K
authors.initials: K
authors.lastname: Haldar
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D015415:Biomarkers; D000939:Epitopes; D004912:Erythrocytes / Q000469:parasitology*; D006801:Humans; D008970:Molecular Weight; D010449:Peptide Mapping; D010963:Plasmodium falciparum / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*; D015800:Protozoan Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D015415:Biomarkers; D000939:Epitopes; D015800:Protozoan Proteins