title: A modified method for the detection of nucleolar organizer regions (AgNORs).
abstract: A modified method for the detection of argyrophilic nucleolar organizer regions (AgNORs) is described. By combination of the original silver staining with a Feulgen staining at the same specimen both the AgNORs and the nuclei of the cells under study are sufficiently contrasted, so that a partial automated counting and measurement of these structures can be done using a computerized microscope image analysis system.
authors.affiliation: Institute of Pathology, Humboldt University, Berlin, F.R.G.
authors.forename: G
authors.initials: G
authors.lastname: Korek
authors.forename: H
authors.initials: H
authors.lastname: Martin
authors.forename: K
authors.initials: K
authors.lastname: Wenzelides
mesh_terms: D000818:Animals; D001254:Astrocytoma / Q000648:ultrastructure; D004396:Coloring Agents; D005909:Glioblastoma / Q000648:ultrastructure; D006651:Histocytochemistry; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D051381:Rats; D012394:Rosaniline Dyes; D012834:Silver; D013194:Staining and Labeling; D001749:Urinary Bladder Neoplasms / Q000648:ultrastructure
chemical_list: D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D012834:Silver