title: Dimensional changes in cells and tissues during specimen preparation for the electron microscope.
abstract: Studies on dimensional changes incurred during preparation of tissue specimens for the transmission and scanning electron microscopes are reviewed, with emphasis on quantitative measurements pertinent to morphometry and three-dimensional reconstruction. The scope of the review includes fixation, dehydration, plastic embedment, critical-point drying, and freeze-drying. Recommendations are presented for monitoring dimensional changes; a strategy for the choice of method of specimen preparation is outlined.
authors.affiliation: Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
authors.forename: M V
authors.initials: MV
authors.lastname: King
mesh_terms: D016477:Artifacts; D002477:Cells / Q000648:ultrastructure*; D005612:Freeze Drying; D016591:Histocytological Preparation Techniques; D008854:Microscopy, Electron / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D016610:Tissue Embedding; D014021:Tissue Preservation