title: Characterization and pharmacological studies of an octopamine-sensitive adenylate cyclase from nerve cord of Locusta migratoria.
abstract: An octopamine-sensitive adenylate cyclase that is insensitive to stimulation by dopamine (DA) and 5-hydroxytryptamine (5-HT), was studied in a homogenized nerve cord preparation of the migratory locust, Locusta migratoria. The enzyme complex is similar to catecholamine-sensitive cyclases from mammals with respect to pH-optimum, requirement for Mg++ and GTP, and sensitivity to forskolin. The octopamine-mediated elevation of adenylate cyclase activity is antagonized by a variety of drugs with the following order of potency: mianserin greater than phentolamine greater than promethazine greater than gramine greater than cyproheptadine greater than cis-flupenthixol greater than chlorpromazine greater than metoclopramide.
authors.affiliation: Department of Biology, University of Waterloo, Ontario, Canada.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Wang
authors.forename: R G
authors.initials: RG
authors.lastname: Downer
authors.forename: J W
authors.initials: JW
authors.lastname: Gole
authors.forename: G L
authors.initials: GL
authors.lastname: Orr
mesh_terms: D000262:Adenylyl Cyclases / Q000187:drug effects*; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004298:Dopamine / Q000494:pharmacology; D006110:Grasshoppers / Q000187:drug effects / Q000201:enzymology*; D066298:In Vitro Techniques; D008297:Male; D009412:Nerve Fibers / Q000187:drug effects / Q000201:enzymology; D009655:Octopamine / Q000494:pharmacology*; D012701:Serotonin / Q000494:pharmacology
chemical_list: D009655:Octopamine; D005576:Colforsin; D012701:Serotonin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D004298:Dopamine