A sensitive fluorometric method for measurement of vascular permeability in spinal cord injury.
A sensitive fluorometric method was modified for the evaluation of drug action upon vascular permeability in rat spinal cord injury. Fluorescein isothiocyanate-conjugated dextran (FITC-D MW 71,200), used as a macromolecular tracer, was injected iv 2 hours before sacrifice. The optimal pH for FITC-D fluorescence was 8.2. The recovery in spinal cord was 101.4 +/- 4.0% (mean +/- SD). The extent of FITC-D extravasation, expressed as the vascular injury index (VII), was increased in proportion to the trauma force. The peak of VII after trauma was at 2 hours. This fluorometric method is sensitive, simple, and reliable for evaluation of drug effects upon vascular permeability in CNS trauma.
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Z X
ZX
Department of Neurology, Medical University of South Carolina, Charleston.
Xu
J
J
Perot
P L
PL
Hogan
E L
EL
D000818:Animals; D002199:Capillary Permeability; D003911:Dextrans; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins; D006863:Hydrogen-Ion Concentration; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000098:blood supply; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology*; D013314:Stress, Mechanical
D003911:Dextrans; D005452:Fluoresceins; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate