title: In vitro HIV infection of primate lymphocytes.
abstract: Peripheral blood mononuclear cells (PBMC) obtained from four different primate species were tested for their respective ability to support the "in vitro" replication of the human immunodeficiency viruses, HIV-1, and HIV-2. PBMC of Cebus apella, patas (Erythrocebus patas), green (cercopithecus aethiops sabaeus) and rhesus monkeys (Macaca mulatta) were infected "in vitro" with either HIV-1 or HIV-2. Cultures were assayed weekly for particle-associated reverse transcriptase activity. Both viruses were found to be cytolytic for all these monkey's PBMC. Low levels of HIV-1 and HIV-2 infection were observed in Cebus cells. However, productive infection was only detected in HIV-2 infected rhesus PBMC. The capacity of HIV-2 to replicate in rhesus cells may provide a useful model for evaluating antiviral drugs and vaccines.
authors.affiliation: Department of Microbiology and Medical Zoology, University of Puerto Rico, San Juan 00936.
authors.forename: E N
authors.initials: EN
authors.lastname: Kraiselburd
authors.forename: D C
authors.initials: DC
authors.lastname: Williams
authors.forename: M J
authors.initials: MJ
authors.lastname: Kessler
mesh_terms: D000818:Animals; D002428:Cebus / Q000382:microbiology; D002470:Cell Survival; D002478:Cells, Cultured; D002522:Chlorocebus aethiops / Q000382:microbiology; D004901:Erythrocebus patas / Q000382:microbiology; D015497:HIV-1 / Q000502:physiology*; D015498:HIV-2 / Q000502:physiology*; D000882:Haplorhini / Q000382:microbiology*; D007963:Leukocytes, Mononuclear / Q000201:enzymology / Q000382:microbiology*; D008253:Macaca mulatta / Q000382:microbiology; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D014779:Virus Replication
chemical_list: D012194:RNA-Directed DNA Polymerase