Immunochemical analysis of high molecular-weight human salivary mucins (MG1) using monoclonal antibodies.
Using four Mabs with different specificities for salivary mucins, an ELISA has been developed in which human whole saliva, glandular salivas, salivary protein fractions and purified, high molecular-weight, mucin fractions (MG1) isolated from human submandibular and sublingual glandular tissues have been immunochemically analysed. All four Mabs reacted with MG1s. Three of them reacted with the purified, low molecular-weight salivary mucins (MG2). None was reactive with parotid saliva. MG1 preparations isolated from submandibular and sublingual glandular tissues of one and the same individual displayed different patterns of reactivity with these Mabs, indicating that they differ immunochemically. Analysis of the MG1s in salivas derived from individual salivary glands showed differences in immunochemical composition. These results indicate that the MG1 fraction in human whole saliva consists of several immunochemically different species.
Veerman
E C
EC
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit, The Netherlands.
Valentijn-Benz
M
M
van den Keybus
P A
PA
Rathman
W M
WM
Sheehan
J K
JK
Nieuw Amerongen
A V
AV
D000017:ABO Blood-Group System / Q000032:analysis; D000116:Acetylgalactosamine / Q000032:analysis; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D009077:Mucins / Q000032:analysis* / Q000302:isolation & purification; D009438:Neuraminic Acids / Q000032:analysis; D010306:Parotid Gland / Q000378:metabolism; D012470:Salivary Glands, Minor / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000302:isolation & purification; D013361:Sublingual Gland / Q000378:metabolism; D013363:Submandibular Gland / Q000378:metabolism
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D009077:Mucins; D009438:Neuraminic Acids; D012471:Salivary Proteins and Peptides; D005643:Fucose; D000116:Acetylgalactosamine; D005690:Galactose