Lasting loss in substance P following administration of substance P antiserum to newborn rats. An immunohistochemical study.
Substance P (SP) antiserum (500 micrograms protein) was administered to rats on the second day of life and the animals were sacrificed 3 months later. This treatment produced a loss in SP immunoreactive fibers in the dorsal horn of the spinal cord, in the substantia nigra and in the periaqueductal gray matter, when compared to control animals receiving a neonatal treatment of non-specific immunoglobulins. In the dorsal horn, the observed depletion was greater in the superficial layers, lamina I and lamina IIo. Immunoreactivity for Met-enkephalin was apparently unchanged by SP antiserum. Results of this study provide cytochemical evidence for a specific and lasting deleterious effect of SP antiserum on different SP-containing neuronal systems.
Achaval
M
M
Department of Chemical Neuroanatomy, Cajal Institute, CSIC, Madrid, Spain.
Martínez-Murillo
R
R
Rodrigo
J
J
Aguado
F
F
De Felipe
M C
MC
Del Río
J
J
D000818:Animals; D000831:Animals, Newborn; D004744:Enkephalin, Methionine / Q000032:analysis; D007106:Immune Sera / Q000008:administration & dosage*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000008:administration & dosage; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000502:physiology* / Q000648:ultrastructure; D010487:Periaqueductal Gray / Q000502:physiology; D051381:Rats; D012016:Reference Values; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*; D013373:Substance P / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D013378:Substantia Nigra / Q000166:cytology / Q000502:physiology*
D007106:Immune Sera; D007074:Immunoglobulin G; D013373:Substance P; D004744:Enkephalin, Methionine