Preliminary X-ray investigation of a bifunctional inhibitor from Indian finger millet (ragi).
A bifunctional alpha-amylase/trypsin inhibitor that has two binding sites has been purified from ragi. The inhibitor has been crystallized from its ammonium sulphate solution by the vapour diffusion method. The crystals belong to the orthogonal space group P2(1)2(1)2(1) with unit cell dimensions a = 30.49 A, b = 56.30 A, c = 73.65 A and Z = 4.
Srinivasan
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Department of Biophysics, All India Institute of Medical Sciences, New Delhi.
Raman
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Singh
T P
TP
D000681:Amylases / Q000037:antagonists & inhibitors*; D001665:Binding Sites; D003432:Cross-Linking Reagents; D006109:Poaceae / Q000737:chemistry*; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D014961:X-Ray Diffraction
D003432:Cross-Linking Reagents; D014361:Trypsin Inhibitors; D000681:Amylases