Establishment of a human cell line highly expressing endothelin in serum-free medium.
Human preproendothelin-1 (prepro-ET-1) was transfected into an immortal human endothelial cell line that had been cultivated in a serum-free medium. Several transformants selected with Ecogpt selection were probed to have prepro-ET insert, express prepro-ET-1 mRNA, and secrete immunoreactive ET (ir-ET). One of the transformants, t-HUE2-1, secreted 30 times the amount of ET-1 and the ratio of mature ET-1 to big ET-1 was 11-fold higher compared with normal human endothelial cells. Thus, this transformant might be of use to study regulation of the posttranscriptional process of prepro-ET-1 in human cells.
Kondo
M
M
Cell Science and Technology Division, Fermentation Research Institute, Agency of Industrial Science and Technology, Ibaraki, Japan.
Ishida
N
N
Kobayashi
M
M
Mitsui
Y
Y
D002460:Cell Line; D003470:Culture Media; D004558:Electric Stimulation; D019332:Endothelin-1; D016232:Endothelins / Q000096:biosynthesis* / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism / Q000502:physiology; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D011247:Pregnancy; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection; D014170:Transformation, Genetic; D014471:Umbilical Veins / Q000166:cytology
D003470:Culture Media; D019332:Endothelin-1; D016232:Endothelins; D011498:Protein Precursors