title: Platelet-activating factor acetylhydrolase in human seminal plasma.
abstract: OBJECTIVE
  To search for the presence of platelet-activating factor (PAF) acetylhydrolase activity in human seminal plasma.
  
  
  METHODS
  Experimental.
  
  
  METHODS
  Reproductive laboratory in a university-affiliated hospital research center.
  
  
  METHODS
  Human male volunteers were selected on the basis of apparent normal health.
  
  
  RESULTS
  Human seminal plasma contained significant levels of PAF-acetylhydrolase activity. Enzymatic hydrolysis of PAF displayed typical kinetics and was a calcium-independent process. Platelet-activating factor acetylhydrolase in seminal plasma was associated with a very high-density lipoprotein fraction. Enzymatic activity was independent of sperm count and motility.
  
  
  CONCLUSIONS
  Human seminal plasma contains significant levels of PAF acetylhydrolase. This enzyme may be an important factor in the regulation of PAF concentration and activity.
authors.affiliation: University of Montreal, Maisonneuve-Rosemont Hospital Research Center, Quebec, Canada.
authors.forename: E D
authors.initials: ED
authors.lastname: Letendre
authors.forename: P
authors.initials: P
authors.lastname: Miron
authors.forename: K D
authors.initials: KD
authors.lastname: Roberts
authors.forename: J
authors.initials: J
authors.lastname: Langlais
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D006801:Humans; D007700:Kinetics; D008074:Lipoproteins / Q000032:analysis / Q000097:blood; D008297:Male; D010741:Phospholipases A / Q000097:blood / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000378:metabolism; D012016:Reference Values; D012661:Semen / Q000737:chemistry / Q000201:enzymology*; D013076:Sperm Count; D013081:Sperm Motility
chemical_list: D008074:Lipoproteins; D010972:Platelet Activating Factor; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase