title: Iron entry route in horse spleen apoferritin. Involvement of the three-fold channels as probed by selective reaction of cysteine-126 with the spin label 4-maleimido-tempo.
abstract: Apoferritin has been selectively labeled with a maleimide nitroxide derivative at Cys-126, located in the hydrophilic 3-fold channels. Titration of this derivative with Fe(II), which gives rise to the initial Fe(III)-apoferritin complex, produces, at low metal-to-protein ratios, a decrease of the intensity of the label EPR signal due to the occurrence of a magnetic dipolar interaction. A label-metal distance ranging between 8-12 A can be estimated from titrations performed with VO(IV), which is known to bind in the 3-fold channels, and likewise produces a decrease in the label EPR signal. The present findings indicate that iron binds in the hydrophilic channels in its higher oxidation state and that these channels represent the metal entry route at least at low metal-to-protein ratios.
authors.affiliation: Department of Organic and Biological Chemistry, University of Messina, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Desideri
authors.forename: S
authors.initials: S
authors.lastname: Stefanini
authors.forename: F
authors.initials: F
authors.lastname: Polizio
authors.forename: R
authors.initials: R
authors.lastname: Petruzzelli
authors.forename: E
authors.initials: E
authors.lastname: Chiancone
mesh_terms: D000818:Animals; D001052:Apoferritins / Q000378:metabolism*; D002104:Cadmium / Q000494:pharmacology; D003497:Cyclic N-Oxides; D003545:Cysteine; D004578:Electron Spin Resonance Spectroscopy; D006736:Horses; D007473:Ion Channels / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D013113:Spin Labels; D013154:Spleen / Q000737:chemistry*; D014638:Vanadates / Q000494:pharmacology
chemical_list: D003497:Cyclic N-Oxides; D007473:Ion Channels; D013113:Spin Labels; D002104:Cadmium; C017480:4-maleimido-2,2,6,6-tetramethylpiperidinooxyl; D014638:Vanadates; D001052:Apoferritins; D007501:Iron; D003545:Cysteine