Phosphorylation of nuclear protein is an early event in TGF beta 1 action.
Transforming growth factor beta (TGF beta) is a family of polypeptides that modulate growth and differentiation. TGF beta exerts its effects on target cells through interaction with specific cell surface receptors, but the signal transduction pathways are as yet largely unresolved. In this study we report that the growth inhibitory action of TGF beta on mink lung CCl 64 cells is associated with a rapid and transient phosphorylation of a number of nuclear proteins. In parallel, a transient expression of the immediate early gene jun B is observed. The expression of jun B can be inhibited by the protein kinase inhibitor H7 and can be augmented by the phosphatase inhibitor okadaic acid. Thus, protein phosphorylation can be a possible mechanism through which TGF beta 1 initiates early genomic responses.
Kramer
I M
IM
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Koornneef
I
I
de Vries
C
C
de Groot
R P
RP
de Laat
S W
SW
van den Eijnden-van Raaij
A J
AJ
Kruijer
W
W
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D004988:Ethers, Cyclic / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D008168:Lung; D008907:Mink; D009687:Nuclear Proteins / Q000378:metabolism*; D019319:Okadaic Acid; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation; D010879:Piperazines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D011519:Proto-Oncogenes / Q000187:drug effects; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D014157:Transcription Factors / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D004268:DNA-Binding Proteins; D004988:Ethers, Cyclic; D007546:Isoquinolines; D009687:Nuclear Proteins; D010710:Phosphates; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D019319:Okadaic Acid; D012313:RNA; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine