title: Mechanisms of molecular recognition of tRNAs by aminoacyl-tRNA synthetases.
abstract: Interactions of Escherichia coli isoleucyl- and glutamyl-tRNA synthetases and their cognate tRNAs were analyzed by phosphate-alkylation mapping with N-nitroso-N-ethylurea and/or by 1H-NMR analysis. When E. coli tRNA(Ile) was bound with isoleucyl-tRNA synthetase, many of the phosphate groups in the anticodon loop and stem and in the D-stem were protected from alkylation. This result is consistent with that of analysis of imino proton resonances due to the secondary and tertiary base pairs. These analyses also suggested that the L-shaped tertiary structure of tRNA(Ile) is distorted upon complex formation with IleRS because of disruption of some tertiary base pairs. In the case of E. coli tRNA(Glu), several phosphate groups in the D-stem and the variable loop were significantly protected by the cognate synthetase. These results indicate that the two tRNAs, unlike other tRNAs studied so far, have some of the "identity determinants" in the D-stem and/or in the anticodon stem.
authors.affiliation: Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Nureki
authors.forename: M
authors.initials: M
authors.lastname: Tateno
authors.forename: T
authors.initials: T
authors.lastname: Niimi
authors.forename: T
authors.initials: T
authors.lastname: Kohno
authors.forename: T
authors.initials: T
authors.lastname: Muramatsu
authors.forename: H
authors.initials: H
authors.lastname: Kanno
authors.forename: Y
authors.initials: Y
authors.lastname: Muto
authors.forename: R
authors.initials: R
authors.lastname: Giege
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D000478:Alkylation; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000926:Anticodon; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D000926:Anticodon; D012329:RNA, Bacterial; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases