[Determination of the circulating antibodies to cardiomyocyte membrane proteins based on the immunoblotting principle].
Cardiomyocyte membrane proteins (CMP) were isolated from the hearts of subjects dead from injuries or brain tumors by differentiated centrifugation in sucrose density gradient for the determination of anti-CMP antibodies in the blood serum samples of myocarditis patients. CMP preparation reactivity with blood serum antibodies of patients with myocarditis and other myocardial diseases, as well as with systemic lupus erythematosus, was assessed by the immune blotting technique. The findings evidence a high incidence of antibody interaction with CMP with a molecular mass of 67 kD. The method is highly specific and sensitive, its results are fairly well reproducible, and the technique is simple.
Tereshchenko
S E
SE
Riazanov
S L
SL
Zimin
Iu I
IuI
Kotelevtsev
Iu V
IuV
D000917:Antibody Formation; D009202:Cardiomyopathies / Q000276:immunology*; D003327:Coronary Disease / Q000276:immunology; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D009206:Myocardium / Q000276:immunology*
D008565:Membrane Proteins
Opredelenie tsirkuliruiushchikh antitel k belkam membrany kardiomiotsitov na printsipe immunoblottinga.