[Binding of monoclonal antibodies to an insulin-producing rat tumor cell (RIN) after partial cell synchronization].
Murine monoclonal antibodies against pancreatic islet cell antigens were generated by somatic cell hybridization and their binding to the insulin-producing rat insulinoma cell line (RIN-5 AH) was evaluated in an indirect immunofluorescence test. An ascites dilution of 1:50 was used for immunostaining using RIN cells harvested from monolayer cultures in RPMI 1640 supplemented with 7.5% fetal calf serum (controls) or test cultures supplemented with 10 mmol/l hydroxyurea to collect the cells in G1/S phase. The five mc-ICSA tested showed a wide variety in their individual binding from 95 up to 30% but their binding was not influenced by hydroxyurea use for synchronization. The antigenic determinant recognized by the anti-glucolipid mc-ICSA 56a F3 was not present on all RIN-5 AH cells. In average, only 35% of the RIN cells showed a striking binding of the monoclonal 56a F3.
Ziegler
B
B
Zentralinstitut für Diabetes Gerhardt Katsch, Karlsburg, DDR.
Schlosser
M
M
Witt
S
S
Bürgelt
P
P
Ziegler
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D002460:Cell Line; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007340:Insulinoma / Q000276:immunology / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000276:immunology / Q000473:pathology*; D051381:Rats
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000939:Epitopes
Untersuchungen zur Bindung monoklonaler Antikörper an einer insulinproduzierenden Rattentumorzellinie (RIN) nach partieller Zellsynchronisierung.