title: Sequence, ontogeny, and cellular localization of murine surfactant protein B mRNA.
abstract: We have isolated and characterized murine surfactant protein (SP-B) cDNAs and determined both the cellular distribution and developmental expression of SP-B mRNA. The nucleotide sequence of the composite SP-B cDNAs and cloned genomic SP-B DNA predicted a primary translation product of 377 amino acids (molecular mass = 41.7 kDa) that contained a single asparagine linked glycosylation site. The 410 base 3' untranslated region contained a novel sequence consisting of a (CCA)21 repeat. Tissue specificity and developmental expression of SP-B mRNA was assessed by Northern blot analysis. A single 1.6-kb mRNA species was detected specifically in lung tissue. In fetal mouse lung SP-B mRNA was detected at low levels on day 15 of gestation and increased markedly between days 16 and 17, reaching adult levels by birth. In situ hybridization analysis of SP-B mRNA demonstrated SP-B expression primarily in bronchiolar and alveolar epithelium, although discrete bronchial cells also contained the SP-B mRNA. The murine SP-B mRNA was expressed in proximal and distal epithelial cells within the respiratory tract and shares a close structural relationship to SP-B from other species.
authors.affiliation: Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, Ohio 45229.
authors.forename: M A
authors.initials: MA
authors.lastname: D'Amore-Bruno
authors.forename: K A
authors.initials: KA
authors.lastname: Wikenheiser
authors.forename: J E
authors.initials: JE
authors.lastname: Carter
authors.forename: J C
authors.initials: JC
authors.lastname: Clark
authors.forename: J A
authors.initials: JA
authors.lastname: Whitsett
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D005333:Fetus / Q000378:metabolism; D016678:Genome; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011510:Proteolipids / Q000235:genetics*; D011663:Pulmonary Surfactants / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D011510:Proteolipids; D011663:Pulmonary Surfactants; D012333:RNA, Messenger; D004247:DNA