[Demonstration of DNA tumor viruses in carcinomas of the upper respiratory tract].
In situ hybridization (ISH) using 35S-labeled probes was applied to the detection of viral DNAs in 115 epithelial tumours from various sites. Epstein-Barr virus (EBV) DNA was detected in 83% of undifferentiated nasopharyngeal carcinomas (NPC) while none of seven squamous cell NPC displayed an EBV-specific autoradiographic signal. Also, all other tumours investigated, including thymomas, thymic carcinomas, tonsillar carcinomas and medullary breast carcinomas were negative upon ISH to EBV-specific probes. These results suggest a unique association of undifferentiated NPC with EBV. 28 tonsillar carcinomas were further analyzed for the presence of human papillomavirus (HPV) DNA. Six carcinomas showed an HPV16-specific signal while hybridization to HPV6- and HPV11-specific probes yielded negative results. No HPV-DNA was detected in any of 30 tonsils with chronic inflammation, thus excluding that the finding of HPV16 in a proportion of tonsillar carcinomas is merely circumstantial. These results point to a possible etiologic role of HPV16 in the pathogenesis of some tonsillar carcinomas.
Niedobitek
G
G
Institut für Pathologie, Klinikum Steglitz, Freie Universität Berlin.
Herbst
H
H
Pitteroff
S
S
Hansmann
M
M
Dienemann
D
D
Hartmann
C A
CA
Finn
T
T
Stein
H
H
D004263:DNA Tumor Viruses / Q000302:isolation & purification*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology / Q000473:pathology; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D012142:Respiratory Tract Neoplasms / Q000382:microbiology* / Q000473:pathology; D014067:Tonsillar Neoplasms / Q000382:microbiology / Q000473:pathology
Nachweis von DNS Tumorviren in Karzinomen des oberen Respirationstraktes.