Characterization of a new minor lymphocyte stimulatory system. I. Cluster of self antigens recognized by "I-E-reactive" V beta s, V beta 5, V beta 11, and V beta 12 T cell receptors for antigen.
In the mouse, two sets of V beta gene products have been shown to be associated with T cell recognition of endogenous self Ag. One of these is the set of V beta associated with T cell reactivities to stimulatory Mls gene products, Mlsa (V beta 6, V beta 8.1, V beta 9) or Mlsc (V beta 3); another is the set of V beta, such as V beta 5, V beta 11, V beta 12, or V beta 17a, which were originally found to be related to I-E recognition. Although the Mls system has been well characterized, little is known about the nature of the ligands for the second set of V beta. In this work, we describe the evidence that the natural ligand or ligands of V beta 5, V beta 11, and V beta 12 may be novel Mls determinants that are recognized by naive T cells at a high precursor frequency and function as the ligand for clonal deletion of self-reactive T cells by negative selection. However, surprisingly, unlike the conventional Mls system, in which all V beta associated with Mlsa recognition or Mlsc recognition are uniformly deleted in those animals expressing the relevant Mls type, expression of these three V beta segregates independently among strains. Based on these observations, the nature of T cell recognition for this new Mls gene product(s) is discussed.
Abe
R
R
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Kanagawa
O
O
Sheard
M A
MA
Malissen
B
B
Foo-Phillips
M
M
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D015152:Blotting, Northern; D002455:Cell Division / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D005434:Flow Cytometry; D006183:H-2 Antigens / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D066298:In Vitro Techniques; D008212:Lymphocyte Depletion; D051379:Mice; D008815:Mice, Inbred Strains; D016747:Minor Lymphocyte Stimulatory Antigens; D012313:RNA / Q000032:analysis; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013154:Spleen / Q000378:metabolism
D000954:Antigens, Surface; D001324:Autoantigens; D000939:Epitopes; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D016747:Minor Lymphocyte Stimulatory Antigens; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D012313:RNA