Expression of protein disulfide isomerase in cultured rat liver epithelial cells is unrelated to the growth inhibitory effect of TGF-beta 1.
The effect of TGF-beta 1 treatment on the level of protein disulfide isomerase (PDI) mRNA in normal and chemically or spontaneously transformed rat liver epithelial cell lines was investigated. TGF-beta 1 at 1 or 10 ng/ml concentrations did not significantly decrease the mRNA level of PDI at 4 or 24 hours after exposure to TGF-beta 1, irrespective whether the cell line was sensitive or resistant to the growth-inhibitory effect of TGF-beta 1 at these concentrations. The results indicate that in normal or neoplastic rat liver epithelial cells, the expression of PDI is unrelated to the growth inhibitory effect of TGF-beta 1.
Tsao
M S
MS
Department of Pathology, Montreal General Hospital, Quebec, Canada.
Grisham
J W
JW
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D015342:DNA Probes; D004261:DNA Replication / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D007535:Isomerases / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D019704:Protein Disulfide-Isomerases; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D011916:Rats, Inbred F344; D013936:Thymidine / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D015342:DNA Probes; D016212:Transforming Growth Factor beta; D012313:RNA; D007535:Isomerases; D019704:Protein Disulfide-Isomerases; D013936:Thymidine