Metabolic and ultrastructural characteristics of biosynthetic processes in the cerebral cortex at 3 months following a prolonged hemorrhagic shock.
Biochemical indicators (the content of ATP, total RNA and DNA, acid and alkaline phosphatase activity) and ultrastructural changes in cells of the nerve tissue were studied in the cerebral cortex of anesthetized dogs 3 months after they had sustained a 4-h hemorrhagic shock (arterial pressure = 40 mmHg). A new variant of reconstruction of cell membranes and organelles that develops in neurons and gliocytes of the brain in the process of adaptation 3 months after resuscitation is identified. This reconstruction variant is described by (a) the presence of a monolayer of a substance with a medium electron density with the incorporated dense granules instead of the common three-layer organisation; (b) the ability displayed by the organellar structure to detect "errors" in its organisation; (c) changes in the character of intercellular relations. The development of this variant of cell structure in the postresuscitation period is likely to be based on the information disintegration of a cell as a system, resulting in distortion of biosynthesis of supracellular ensembles and biological membranes of nerve cells in particular.
Kozhura
V L
VL
Institute of General Resuscitology, U.S.S.R. Academy of Medical Sciences, Moscow.
Solov'eva
Zh V
ZhV
Novoderzhkina
I S
IS
Smirnova
N V
NV
D000135:Acid Phosphatase / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001253:Astrocytes / Q000648:ultrastructure; D001923:Brain Chemistry / Q000502:physiology*; D002540:Cerebral Cortex / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis; D004285:Dogs; D008854:Microscopy, Electron; D009474:Neurons / Q000648:ultrastructure; D009836:Oligodendroglia / Q000648:ultrastructure; D012313:RNA / Q000032:analysis; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
D012313:RNA; D000255:Adenosine Triphosphate; D004247:DNA; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase