title: Purification, characterization, and acute phase response of plasma alpha-1-antiproteinase in the hamster, Mesacricetus auratus.
abstract: 1. alpha-1-Antiproteinase (also called alpha-1-antitrypsin or alpha-1-proteinase inhibitor) with a molecular mass of 60 kDa was purified to apparent homogeneity from hamster plasma. 2. It inhibited elastase, chymotrypsin and trypsin, but did not significantly affect pancreatic kallikrein, plasma kallikrein or plasmin. 3. It has the same N-terminal heptapeptide sequence as that of rat alpha-1-antiproteinase. 4. Its plasma level decreased after injection of bacterial lipopolysaccharide.
authors.affiliation: Research Institute of Oriental Medicine, Kinki University School of Medicine, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Amemiya
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Sinohara
mesh_terms: D000209:Acute-Phase Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000210:Acute-Phase Reaction / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D006224:Cricetinae; D005341:Fibrinolysin / Q000378:metabolism; D007525:Isoelectric Focusing; D007610:Kallikreins / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D008647:Mesocricetus; D008969:Molecular Sequence Data; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D016415:Sequence Alignment; D014361:Trypsin Inhibitors / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000209:Acute-Phase Proteins; D008070:Lipopolysaccharides; D014361:Trypsin Inhibitors; D000515:alpha 1-Antitrypsin; D007610:Kallikreins; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D005341:Fibrinolysin