title: Characterization of a 70Z/3 pre-B cell derived macrophage clone. Differential expression of Hox family genes.
abstract: An adherent cell line was established from the much studied pre-B cell line 70Z/3. The adherent cell line had the morphological features of a macrophage and stained positive for non-specific esterase. In addition, this line expressed high levels of RNA for the macrophage specific enzyme, lysozyme. Although the 70Z/3 macrophage variant has lost the ability to respond to lipopolysaccharide and interferon-gamma by expressing RNA transcripts for immunoglobulin light chain, it exhibited a new response characterized by increased levels of I-A RNA transcripts. Both the pre-B and macrophage variants also expressed RNA transcripts which hybridize to a Hox 2.3 probe. In contrast, transcripts which hybridize to Hox 1.1, 2.1, and 6.1 probes are expressed in the pre-B line but could not be detected in the macrophage.
authors.affiliation: Ontario Cancer Institute, Toronto, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Hara
authors.forename: M
authors.initials: M
authors.lastname: Sam
authors.forename: R A
authors.initials: RA
authors.lastname: Maki
authors.forename: G E
authors.initials: GE
authors.lastname: Wu
authors.forename: C J
authors.initials: CJ
authors.lastname: Paige
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000201:enzymology / Q000276:immunology; D002448:Cell Adhesion; D002999:Clone Cells / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D008264:Macrophages / Q000166:cytology* / Q000201:enzymology / Q000276:immunology; D015335:Molecular Probes; D012313:RNA / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D015335:Molecular Probes; D012313:RNA