title: Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli.
abstract: Enzyme-linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli-expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.
authors.affiliation: Virology Division, National Cancer Center Research Institute, Tokyo.
authors.forename: K
authors.initials: K
authors.lastname: Muraiso
authors.forename: M
authors.initials: M
authors.lastname: Hijikata
authors.forename: N
authors.initials: N
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Shimotohno
authors.forename: N
authors.initials: N
authors.lastname: Okazaki
authors.forename: S
authors.initials: S
authors.lastname: Ohkoshi
authors.forename: M
authors.initials: M
authors.lastname: Uura
authors.forename: S
authors.initials: S
authors.lastname: Kaneko
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Omata
mesh_terms: D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics*; D006508:Hepatitis Antibodies / Q000032:analysis; D006526:Hepatitis C / Q000175:diagnosis*; D018937:Hepatitis C Antibodies; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis; D012698:Serologic Tests; D014758:Viral Core Proteins / Q000032:analysis / Q000276:immunology*
chemical_list: D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D012367:RNA, Viral; D014758:Viral Core Proteins