Interleukin 4 drives phytohemagglutinin-activated T cells through several cell cycles: no synergism between interleukin 2 and interleukin 4.
Cell kinetic studies of T cells stimulated with the interleukin 2 (Il-2), Il-4, or both lymphokines were performed with conventional [3H]thymidine incorporation and with the bivariate BrdU/Hoechst technique. Il-2 and Il-4 are able to drive phytohemagglutinin-activated T cells through more than one cell cycle. Neither synergistic nor inhibitory effect on T-cell proliferation was seen for the stimulation with both Il-2 and Il-4 as compared with the effect of Il-2 alone. The quantitative data of the cell cycle distribution of phytohemagglutinin-activated T cells suggest that the population of Il-4-responsive cells is at least an overlapping population, if not a real subset of the population of the Il-2-responsive cells.
Lehrnbecher
T
T
Department of Physiological Chemistry, University of Würzburg, Germany.
Merz
H
H
Sebald
W
W
Poot
M
M
D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*
D007376:Interleukin-2; D010835:Phytohemagglutinins; D015847:Interleukin-4; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole