title: A novel approach to nonradioactive hybridization assay of nucleic acids using stained latex particles.
abstract: The paper describes a sensitive latex hybridization assay (LHA) method applied for indirect detection of biotinylated nucleic acid hybrids immobilized on a synthetic membrane. The biotinylated hybrids were visualized by means of latex particles containing the fluorescent dye pyronine G and coated with streptavidin; 1.6 and 0.3 pg of lambda-phage DNA was detected by dot blot hybridizations on nylon membrane and polyethyleneimine-cellophane, respectively. The assay sensitivity was increased by three orders of magnitude over that with fluorescently labeled probes due to encapsulation of the fluorescent dye in polymer particles. LHA is simple (single-stage detection procedure), fast, and more sensitive than any of the other nonradioactive hybridization methods.
authors.affiliation: Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
authors.forename: T I
authors.initials: TI
authors.lastname: Vener
authors.forename: M F
authors.initials: MF
authors.lastname: Turchinsky
authors.forename: V D
authors.initials: VD
authors.lastname: Knorre
authors.lastname: Lukin YuV
authors.forename: S N
authors.initials: SN
authors.lastname: Shcherbo
authors.forename: V P
authors.initials: VP
authors.lastname: Zubov
authors.forename: E D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D000171:Acrolein; D001426:Bacterial Proteins; D015415:Biomarkers; D001710:Biotin; D002476:Cellophane; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes; D007840:Latex; D008567:Membranes, Artificial; D009693:Nucleic Acid Hybridization; D009757:Nylons; D011094:Polyethyleneimine; D011108:Polymers; D013194:Staining and Labeling; D019809:Streptavidin
chemical_list: D001426:Bacterial Proteins; D015415:Biomarkers; D005456:Fluorescent Dyes; D007840:Latex; D008567:Membranes, Artificial; D009757:Nylons; D011108:Polymers; C029975:polyacrolein; D001710:Biotin; D000171:Acrolein; D011094:Polyethyleneimine; D002476:Cellophane; D004247:DNA; D019809:Streptavidin