Effects of interleukin-6 and leukemia inhibitory factor on the acute phase response and DNA synthesis in cultured rat hepatocytes.
Human recombinant interleukin-6 (IL-6) and human recombinant leukemia inhibitory factor (LIF) similarly stimulate synthesis of typical acute-phase proteins in the primary rat hepatocyte cultures. LIF is, however, less effective in increasing uptake of alpha-aminoisobutyric acid than IL-6. Antiserum to human IL-6 abolishes induced protein synthesis and amino acid uptake elicited by hrIL-6 but has no effect on the acute-phase response of rat liver cells stimulated by LIF. Both IL-6 and LIF inhibit basal and epidermal growth factor-induced DNA synthesis in rat hepatocytes.
Kordula
T
T
Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.
Rokita
H
H
Koj
A
A
Fiers
W
W
Gauldie
J
J
Baumann
H
H
D000209:Acute-Phase Proteins / Q000096:biosynthesis; D000210:Acute-Phase Reaction / Q000139:chemically induced*; D000818:Animals; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D006131:Growth Inhibitors; D015850:Interleukin-6 / Q000494:pharmacology*; D053605:Leukemia Inhibitory Factor; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008222:Lymphokines / Q000494:pharmacology*; D051381:Rats
D000209:Acute-Phase Proteins; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D004247:DNA