title: Macrophage subpopulations in normal and transplanted heart and kidney tissues in the rat.
abstract: The purpose of the present study was to investigate the phenotype of macrophages that infiltrate normal and transplanted rat tissues. The macrophage monoclonal antibodies ED1, ED2, ED3, 52-1D4, ER15, and OX43, together with antibodies against lymphocyte and class II MHC antigens, were used in an indirect immunofluorescence technique with sections of normal tissues and heart and renal grafts that experienced long-term survival or rejection. A small number of ED1- and ED3-positive interstitial cells were detected in normal heart and renal tissues and their number increased dramatically in rejection. Normal heart tissue contained a population of ED2-positive cells with dendritic morphology that was not detected in renal tissue. Following transplantation, a diffuse increase of rounded ED2-positive cells was observed in heart grafts; no ED2-positive cells were detected in grafts removed after 20-30 days from nonimmunosuppressed recipients. Grafts from CsA-treated animals or grafts that survived greater than 50 days in nonimmunosuppressed recipients exhibited the interstitial dendritic pattern of ED2-positive cells. Only very few rounded ED2-positive cells were observed in renal allografts; if present, they were mostly located in the medulla. OX43, which bound in normal tissues to vessel endothelium and a population of macrophages, stained in allografts an additional small population of graft-infiltrating cells, and in F344 renal allografts a population of multinucleated giant cells. We conclude that the posttransplant macrophage infiltration pattern of heart and renal allografts, defined by the monocyte/macrophage antibodies ED1, ED3, 52-1D4, and ER15, is very similar for both types of organs, although the antibody ED2 and the endothelial-macrophage antibody OX43 revealed remarkable differences between the two types of organ allografts.
authors.affiliation: Department of Medicine, University of Calgary, Canada.
authors.forename: L C
authors.initials: LC
authors.lastname: Paul
authors.forename: G T
authors.initials: GT
authors.lastname: Grothman
authors.forename: H
authors.initials: H
authors.lastname: Benediktsson
authors.forename: A
authors.initials: A
authors.lastname: Davidoff
authors.forename: J
authors.initials: J
authors.lastname: Rozing
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D006084:Graft Rejection; D016027:Heart Transplantation / Q000276:immunology*; D016030:Kidney Transplantation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D014184:Transplantation, Homologous
chemical_list: D000911:Antibodies, Monoclonal