The expression of the c-src+ proto-oncogene mRNA in foetal striatal tissue grafts.
We have studied by in situ hybridization the mRNA expression of the pp60c-src proto-oncogene in grafts of foetal striatal neurons implanted into the ibotenic acid lesioned adult rat neostriatum. Animals were studied at 5, 10, 15, 30 days and 4 months after transplantation. Using 35S-labelled specific oligonucleotide probes, high levels of mRNA for pp60c-src+ but not pp60c-src- were found in the normal embryonic striatum and in striatal grafts up to 1 month after implantation; no hybridization signal was obtained in the normal adult striatum or in 4-month old grafts. The data suggest that pp60c-src+ is highly expressed in developing, but not mature, neurons and may play a fundamental role in the maturation and differentiation of grafted embryonic striatal neurons.
Mayer
E
E
Department of Experimental Psychology, University of Cambridge, UK.
Heavens
R P
RP
Dunnett
S B
SB
Sirinathsinghji
D J
DJ
D000818:Animals; D016380:Brain Tissue Transplantation; D003342:Corpus Striatum / Q000187:drug effects / Q000196:embryology / Q000378:metabolism / Q000637:transplantation*; D005260:Female; D016332:Fetal Tissue Transplantation; D015971:Gene Expression Regulation, Enzymologic; D016391:Genes, src; D007051:Ibotenic Acid / Q000633:toxicity; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D007051:Ibotenic Acid; D016392:Proto-Oncogene Proteins pp60(c-src)