title: The efficiency of RNA 3'-end formation is determined by the distance between the cap site and the poly(A) site in spleen necrosis virus.
abstract: The efficiency of RNA 3'-end formation of spleen necrosis virus (SNV) is determined by the distance between the cap site and the poly(A) site. When the distance between the cap site and the poly(A) site was shorter than 500 bases, only 3-9% of the RNA was polyadenylated at the SNV poly(A) site. However, when the distance between the cap site and the poly(A) site was 1400 bases or more, 70% of the total RNA was polyadenylated at the SNV poly(A) site. In contrast, the poly(A) signal sequences of the thymidine kinase (tk) and SV40 late genes functioned at high efficiency, even with a distance between the cap site and the poly(A) site that was short enough to inactivate the SNV poly(A) signal. Therefore, this distance-dependent inactivation of RNA 3'-end formation is specific for SNV sequences and perhaps for related retroviruses. This finding explains the difference between the 5' and 3' poly(A) sites in many retrovirus RNAs.
authors.affiliation: McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
authors.forename: K
authors.initials: K
authors.lastname: Iwasaki
authors.forename: H M
authors.initials: HM
authors.lastname: Temin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D005814:Genes, Viral; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D011061:Poly A / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics*; D012315:RNA Caps / Q000235:genetics*; D012333:RNA, Messenger; D012190:Retroviridae / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics
chemical_list: D015345:Oligonucleotide Probes; D012315:RNA Caps; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA