Enhancement of neutralizing efficacy by combining three monoclonal antibodies to human interferon-alpha.
Three murine monoclonal antibodies (mAb) directed to distinct epitopes on recombinant human interferon (IFN)-alpha 1, and three mAb recognizing distinct epitopes on recombinant human interferon (IFN) alpha 1, and three mAb recognizing distinct epitopes on recombinant human IFN-alpha sc, were studied by IFN-neutralizing assays. The efficacy of neutralization of the anti-viral and the anti-proliferative activities of IFN-alpha 1, or IFN-alpha 2c, by the specific antibodies used, individually or in combination, were evaluated. In comparison with single mAb, the mixtures of three mAb against IFN-alpha 1 or three mAb against IFN-alpha 2c were capable of neutralizing more than 10-times larger amounts of IFN-alpha 1 and alpha 2c, respectively. The strong potentiation of the neutralization efficacy resulting from mixing different mAb was demonstrated by neutralization of the anti-viral as well as the anti-proliferative activities of both recombinant IFN. The neutralization experiments support the interpretation that the observed potentiation results from simultaneous interaction of anti-IFN mAb with different epitope specificity.
Kontsek
P
P
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czech.
Borecký
L
L
Novák
M
M
Kontseková
E
E
Máciková
I
I
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007370:Interferon Type I / Q000276:immunology*; D011994:Recombinant Proteins; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007370:Interferon Type I; D011994:Recombinant Proteins