En bloc staining available for stereoscopic observation of epoxy resin Quetol 651-embedded thick sections under a high voltage transmission electron microscope.
This method has been devised for easy en block staining for stereoscopic observation of thick sections under a high voltage transmission electron microscope (HVTEM). It uses carbohydrazide as an osmium bridging agent and both osmium tetroxide and uranyl acetate as electron staining agents. Osmium tetroxide-fixed and en bloc-stained tissue blocks are embedded in a Quetol 651 resin mixture. Thick sections (2-3 microns thick) without double staining are observed at an accelerating potential of 300 kV and a tilt angle of +/- 10 degrees by an H-9000 TEM with a side-entry goniometer. Stereoscopic electron micrographs can be obtained.
Kushida
T
T
Department of Anatomy, Tokyo Women's Medical College, Japan.
Iijima
H
H
Kushida
H
H
Tsuruta
C
C
D000818:Animals; D004853:Epoxy Resins; D008297:Male; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D008867:Microtomy / Q000379:methods; D012671:Seminiferous Tubules / Q000648:ultrastructure; D013194:Staining and Labeling
D004853:Epoxy Resins; C035364:Quetol 651