Cytogenetic characterization of radiosensitive mouse mutants.
In order to develop mouse models for human mutagen-sensitive syndromes, we carried out cytogenetic characterization of several mouse mutants and MS/Ae mice showing enhanced radiosensitivities. The applied cytogenetic techniques include chromosomal analysis of in vitro cell cultures and lymphocyte cultures as well as in vivo UDS in hepatocytes, induction of micronuclei in polychromatic erythrocytes and translocation induction in spermatogonial stem cells. Among the mutations studied, namely the contrasted allele of steel (Slcon), viable dominant spotting (Wc), wasted (wst), varitint-waddler (Va) and dystonia musculorum (dt) as well as MS/Ae mice, various iso-, hyper- or hypo-sensitive conditions were recorded. Only Va and dt appear to be associated with some deficiency in DNA repair.
van Buul
P P
PP
MGC, Department of Radiation Genetics and Chemical Mutagenesis, Leiden, The Netherlands.
Tuinenburg-Bol Raap
A
A
Goudzwaard
H J
HJ
Seelen
C M
CM
Beechey
C V
CV
Natarajan
A T
AT
Searle
A G
AG
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005838:Genotype; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D011836:Radiation Tolerance / Q000235:genetics*; D013737:Testis / Q000166:cytology / Q000528:radiation effects
D004247:DNA