Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli.
Enzyme-linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli-expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.
Muraiso
K
K
Virology Division, National Cancer Center Research Institute, Tokyo.
Hijikata
M
M
Kato
N
N
Shimotohno
K
K
Okazaki
N
N
Ohkoshi
S
S
Uura
M
M
Kaneko
S
S
Kobayashi
K
K
Omata
M
M
D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics*; D006508:Hepatitis Antibodies / Q000032:analysis; D006526:Hepatitis C / Q000175:diagnosis*; D018937:Hepatitis C Antibodies; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis; D012698:Serologic Tests; D014758:Viral Core Proteins / Q000032:analysis / Q000276:immunology*
D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D012367:RNA, Viral; D014758:Viral Core Proteins