title: Gastrointestinal neuropeptides suppress human colonic lamina propria lymphocyte DNA synthesis.
abstract: Gastrointestinal neuropeptides have been shown to modulate the circulatory immune system, but their effect on the mucosal immune system is not well defined. We studied the effect of VIP, SOM, S-P and Bomb on thymidine incorporation into human colonic lamina propria lymphocyte (LPL) DNA. Physiologic concentrations of VIP, SOM, S-P and Bomb significantly suppressed thymidine incorporation into Con A-stimulated human LPL. These neuropeptides did not affect DNA synthesis when LPL were induced with phorbol ester (PDB) and calcium ionophore (ionomycin). Our data suggest that a) VIP, SOM, S-P, and Bomb may have a regulatory role in the human mucosal immune system, and b) Bomb should be added to the list of neuropeptides which affect the gut immune system.
authors.affiliation: Department of Medicine, Wayne State University, Children's Hospital of Michigan, Detroit 48201.
authors.forename: Y
authors.initials: Y
authors.lastname: Elitsur
authors.forename: G D
authors.initials: GD
authors.lastname: Luk
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001839:Bombesin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003106:Colon / Q000187:drug effects* / Q000276:immunology; D004261:DNA Replication / Q000187:drug effects*; D003864:Depression, Chemical; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000276:immunology; D015759:Ionomycin / Q000494:pharmacology; D008875:Middle Aged; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D013004:Somatostatin / Q000494:pharmacology*; D013373:Substance P / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets; D013601:T-Lymphocytes / Q000187:drug effects*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
chemical_list: D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D015240:Phorbol 12,13-Dibutyrate; D013004:Somatostatin; D015759:Ionomycin; D001839:Bombesin