title: Lymphotoxin and tumor necrosis factor-alpha production by myelin basic protein-specific T cell clones correlates with encephalitogenicity.
abstract: Lymphokine activity in seven myelin basic protein (MBP)-specific T cell clones was examined. All of the clones recognize MBP peptide 1-9 in the context of I-Au. A strong positive correlation was found between levels of lymphotoxin (LT) and tumor necrosis factor alpha (TNF-alpha) mRNA and biological activity on L929 cells and their capacity to induce paralysis, the clinical hallmark of experimental allergic encephalomyelitis (EAE). No correlation was found between interleukin-2 or gamma interferon production and encephalitogenicity. LT and/or TNF-alpha may play a central role in the pathogenesis of EAE.
authors.affiliation: Department of Neurology, Stanford University School of Medicine, CA 94305.
authors.forename: M B
authors.initials: MB
authors.lastname: Powell
authors.forename: D
authors.initials: D
authors.lastname: Mitchell
authors.forename: J
authors.initials: J
authors.lastname: Lederman
authors.forename: J
authors.initials: J
authors.lastname: Buckmeier
authors.forename: S S
authors.initials: SS
authors.lastname: Zamvil
authors.forename: M
authors.initials: M
authors.lastname: Graham
authors.forename: N H
authors.initials: NH
authors.lastname: Ruddle
authors.forename: L
authors.initials: L
authors.lastname: Steinman
mesh_terms: D000818:Animals; D002999:Clone Cells / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology*; D008222:Lymphokines / Q000096:biosynthesis*; D008233:Lymphotoxin-alpha / Q000096:biosynthesis; D051379:Mice; D004676:Myelin Basic Protein / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D008222:Lymphokines; D008233:Lymphotoxin-alpha; D004676:Myelin Basic Protein; D014409:Tumor Necrosis Factor-alpha