Isolation and structural characterization of the Chlamydomonas reinhardtii gene for cytochrome c6. Analysis of the kinetics and metal specificity of its copper-responsive expression.
We have isolated a 5-kilobase pair fragment of genomic DNA containing the entire coding region for the Chlamydomonas reinhardtii gene encoding the copper-repressible Cyt c6. A region comprising 2.6 kilobase pairs contains the entire transcribed region plus 852 nucleotides upstream of the Cyt c6 transcription start site and 495 nucleotides downstream of the conserved C. reinhardtii polyadenylation signal. Comparison of the genomic sequence with the cDNA sequence (Merchant, S., and Bogorad, L. (1987) J. Biol. Chem. 262, 9062-9067) revealed that the coding region is interrupted by two introns, each of which is flanked by C. reinhardtii consensus intron/exon boundaries. Primer extension and S1 nuclease protection analyses identified the 5' border of the Cyt c6 mRNA at approximately 79 base pairs upstream from the initiator methionine. Analysis of the 5' upstream region reveals no significant similarity to sequences found in upstream regions of other copper-regulated genes. Time-course studies indicate that 1) the mature Cyt c6 mRNA has a half-life of approximately 45-60 min and is completely lost within 4 h, and 2) the primary, unspliced transcript has a half-life of approximately 10 min and is completely lost within 30 min after the addition of copper ions to copper-depleted cells. These results indicate that the response to copper occurs very rapidly upon elevation of extracellular copper levels. Although this gene is unresponsive to silver ions in vivo, in contrast to the yeast copper-responsive CUP1 gene (Furst, P., Hu, S., Hackett, R., and Hamer, D. (1988) Cell 55, 705-717), it does respond to mercury ions, albeit with less sensitivity. Mercury ions cannot, however, substitute for copper in allowing the accumulation of plastocyanin in vivo.
Hill
K L
KL
Department of Chemistry and Biochemistry, UCLA 90024.
Li
H H
HH
Singer
J
J
Merchant
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002696:Chlamydomonas / Q000201:enzymology / Q000235:genetics*; D003300:Copper / Q000502:physiology*; D003580:Cytochromes / Q000235:genetics*; D045348:Cytochromes f; D004247:DNA / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D017343:Genes, Plant; D007700:Kinetics; D008627:Mercuric Chloride / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology; D008969:Molecular Sequence Data; D010970:Plastocyanin / Q000378:metabolism; D012329:RNA, Bacterial / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D015183:Restriction Mapping; D012835:Silver Nitrate / Q000494:pharmacology; D013379:Substrate Specificity; D014158:Transcription, Genetic
D003580:Cytochromes; D008670:Metals; D012329:RNA, Bacterial; D012333:RNA, Messenger; D008627:Mercuric Chloride; D003300:Copper; D004247:DNA; D010970:Plastocyanin; D045348:Cytochromes f; D012835:Silver Nitrate