[Coupling of bovine gamma globulin to carbonochloridate-activated cellulose beads, CNBr-activated Sepharose CL-4B and NaIO4-oxidized Sepharose 6B and use of the coupled products for immunoaffinity chromatography].
For coupling 25 mg of bovine IgG (BGG) were given to 5 ml volumes of packed bead cellulose activated by 5-norbornene-2.3-dicarboximido carbonochloridate and CNBr-activated Sepharose CL-4B, respectively. Thus, BGG-immunosorbents were obtained with 4.6 to 4.9 mg BGG/ml matrix. 5 ml volumes of packed NaIO4-oxidized Sepharose 6B coupled 27% from 25 mg of BGG, only. In this case, immunosorbents with 1.35 mg BGG/ml matrix were produced. All BGG-immunosorbents were chemically relatively stable. The use of these immunosorbents for affinity chromatography results in the isolation of one milligram of pure rabbit anti-BGG antibodies by means of about 4.6 mg of BGG coupled to the cellulose or the Sepharose-CL-4B matrices. On the other side, only 3.4 mg of BGG coupled to the NaIO4-activated Sepharose 6B were necessary in order to isolate one milligram of antibodies in an immunoelectrophoretically pure state.
Hädge
D
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Bereich Tierphysiologie und Immunbiologie, Karl-Marx-Universität Leipzig.
D000818:Animals; D002417:Cattle; D002482:Cellulose / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D007163:Immunosorbent Techniques; D066298:In Vitro Techniques; D012685:Sepharose / Q000737:chemistry; D005719:gamma-Globulins / Q000737:chemistry*
D005719:gamma-Globulins; D002482:Cellulose; D012685:Sepharose
Kopplung von Rindergammaglobulin an Chlorameisensäureester-aktivierte Perlcellulose, CNBr-aktivierte Sepharose CL-4B und NaIO4-oxidierte Sepharose 6B sowie Einsatz der Kopplungsprodukte zur Immunaffinitätschromatographie.