Isolation of a cDNA clone, encoding a human beta-galactoside binding protein, overexpressed during glucocorticoid-induced cell death.
In this report we describe the isolation and characterization of a cDNA clone overexpressed tenfold during the induction of apoptosis in the glucocorticoid-sensitive human leukaemia cell line CEM C7. This clone was shown by DNA sequence analysis to represent the human HL14 gene, encoding a beta-galactoside binding protein, the mouse homologue of which has recently been reported to act as a cell growth inhibitory factor.
Goldstone
S D
SD
Queensland Cancer Fund Research Unit, Queensland Institute of Medical Research, Herston, Brisbane, Australia.
Lavin
M F
MF
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D003907:Dexamethasone / Q000494:pharmacology*; D005697:Galactosides / Q000378:metabolism; D037161:Galectins; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D015723:Gene Library; D006388:Hemagglutinins / Q000235:genetics*; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification
D002352:Carrier Proteins; D004273:DNA, Neoplasm; D005697:Galactosides; D037161:Galectins; D006388:Hemagglutinins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D003907:Dexamethasone