Specific uptake of asialofetuin-tacked liposomes encapsulating interferon-gamma by human hepatoma cells and its inhibitory effect on hepatitis B virus replication.
Asialofetuin-tacked liposomes (AF-liposomes) encapsulating interferon (IFN)-gamma were bound and internalized into a human hepatoma cell line, HepG2 cells, selectively through asialoglycoprotein receptor, but not non-tacked liposomes (N-liposomes). AF-liposomal IFN-gamma was more effective for inhibition of viral DNA replication in hepatitis B virus (HBV)-producing clone from HepG2 cells transfected with HBV-DNA than N-liposomal IFN-gamma. AF-liposomes may increase the therapeutic potential of IFN-gamma through asialoglycoprotein receptor in treating HBV-infected hepatocytes.
Ishihara
H
H
Tokyo College of Pharmacy, Japan.
Hayashi
Y
Y
Hara
T
T
Aramaki
Y
Y
Tsuchiya
S
S
Koike
K
K
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D001692:Biological Transport; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D004337:Drug Carriers; D060748:Fetuins; D006515:Hepatitis B virus / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D008081:Liposomes; D008113:Liver Neoplasms; D011971:Receptors, Immunologic / Q000378:metabolism*; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D013696:Temperature; D014779:Virus Replication / Q000187:drug effects*; D000509:alpha-Fetoproteins
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D004337:Drug Carriers; D060748:Fetuins; D008081:Liposomes; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D007371:Interferon-gamma