Allergenic fragments in Parietaria judaica pollen extract.
High-performance ion-exchange chromatography and immunoaffinity chromatography suggest that Par jI, the principal allergenic component of Parietaria judaica pollen, is a very unstable molecule, which tends to fragment in solution. Several fragments were obtained from Par jI and some of them show positivity toward the anti-Par jI monoclonal antibody, suggesting that they retain the entire structure of the allergenic determinant. These fragments could be the target for sequence and conformation studies.
Bolzacchini
E
E
Dipartimento di Chimica Organica e Industriale, Università di Milano, Italy.
Di Gennaro
P
P
Di Gregorio
G
G
Rindone
B
B
Falagiani
P
P
Mistrello
G
G
Sondergaard
I
I
D000485:Allergens / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D000939:Epitopes / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007158:Immunologic Techniques; D011058:Pollen / Q000032:analysis* / Q000276:immunology; D011852:Radioallergosorbent Test
D000485:Allergens; D000911:Antibodies, Monoclonal; D000939:Epitopes