IgG- and IgE-mediated histamine release from superfused guinea-pig airway tissues.
Anaphylactic histamine release and the inhibition by the beta-adrenoceptor agonist fenoterol has been investigated using lung and tracheal tissues from two groups of guinea-pigs, differently sensitized to respond with IgG or IgE antibodies, respectively. A superfusion method was introduced and compared with classical batch incubation. The difference between IgG- and IgE-mediated histamine release during superfusion of both tissues was much greater than the difference obtained during batch-incubations. Fenoterol inhibited IgG-mediated histamine release during superfusion at lower concentrations and to a larger extent than the release from IgE-sensitized tissues. The inhibition by fenoterol was less pronounced after batch-wise incubations, preincubation at 0 degrees C abolished the quantitative difference of IgG- and IgE-mediated histamine release from lung slices as well as the difference in beta-adrenergic inhibition. It is concluded that the new superfusion procedure for airway tissues enhances the sensitivity of antigen-induced histamine release for pharmacological modulation, compared with batch-wise incubation. In addition, the effects of 0 degrees C pretreatment show that cooled transport and storage of airway tissue should be considered with care.
Van Amsterdam
R G
RG
Department of Pharmacology, University of Groningen, The Netherlands.
Santing
R E
RE
De Boer
J
J
Brouwer
F
F
Zaagsma
J
J
D000818:Animals; D000906:Antibodies / Q000276:immunology; D005260:Female; D005280:Fenoterol / Q000494:pharmacology*; D006168:Guinea Pigs; D006636:Histamine Release / Q000187:drug effects*; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010477:Perfusion; D014132:Trachea / Q000187:drug effects / Q000502:physiology*
D000906:Antibodies; D007074:Immunoglobulin G; D005280:Fenoterol; D007073:Immunoglobulin E