Endothelial markers in malignant vascular tumours of the liver: superiority of QB-END/10 over von Willebrand factor and Ulex europaeus agglutinin 1.
A new monoclonal antibody, QB-END/10, raised against the CD34 antigen in human endothelial cell membranes and haemopoietic progenitor cells, was studied for its usefulness as a marker of neoplastic vascular cells in 21 angiosarcomas and seven malignant haemangioendotheliomas of the liver. QB-END/10 was both more sensitive and more specific than Von Willebrand factor (VWF) and Ulex europaeus 1 agglutinin (UEA-1) in labelling endothelial cells and it did not cross react with epithelia as UEA-1 often does. Staining was uniformly strong and clear in all histological variants of these two tumours. QB-END/10 should prove particularly useful in the differential diagnosis of malignant vascular tumours of the liver.
Anthony
P P
PP
Area Department of Pathology, Royal Devon Hospital, Postgraduate Medical School, University of Exeter, Heavitree.
Ramani
P
P
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D018952:Antigens, CD34; D000943:Antigens, Differentiation / Q000276:immunology; D014408:Biomarkers, Tumor / Q000276:immunology*; D006390:Hemangioendothelioma / Q000276:immunology*; D006394:Hemangiosarcoma / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins; D008113:Liver Neoplasms / Q000276:immunology*; D037121:Plant Lectins; D013194:Staining and Labeling; D014841:von Willebrand Factor
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D014408:Biomarkers, Tumor; D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D014841:von Willebrand Factor