Comparative analysis of the replication regions of IncB, IncK, and IncZ plasmids.
Minireplicons from the I-complex plasmids R387 (IncK) and pIE545 (IncZ) were constructed, and the nucleotide sequences of their replication regions were compared with that of the B plasmid, pMU720. The coding sequence of the putative replication protein, RepA, of each plasmid was located. RepA of K and B plasmids were homologous, whereas RepA of Z resembled RepA1 of FII plasmid. Sequences upstream of RepA were conserved in the three I-complex plasmids. Group B and Z plasmids were incompatible.
Praszkier
J
J
Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
Wei
T
T
Siemering
K
K
Pittard
J
J
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004265:DNA Helicases; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010957:Plasmids; D011506:Proteins; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D013816:Thermodynamics; D015534:Trans-Activators
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D011506:Proteins; D012329:RNA, Bacterial; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases