Molecular analysis of the Bacillus subtilis recF function.
recF resides between the dnaN and gyrB genes of Bacillus subtilis. The recF15 mutation results in replacement of a glutamate residue in the wild type with a lysine residue in the mutant RecF protein. We investigated the in vivo regulation of recF using a transcriptional fusion to the xylE gene and assaying mRNA production. We found that novobiocin leads to a four-fold induction in recF gene expression, but this is not observed in a gyrB mutant strain. Enhancement of expression of the recF gene in the presence of novobiocin is unrelated to the SOS response. The RecF protein, which has a predicted molecular mass of 42.2 kDa, does not seem to be involved in its own regulation.
Alonso
J C
JC
Max-Planck-Institut für Molekulare Genetik, Berlin, Federal Republic of Germany.
Stiege
A C
AC
D001412:Bacillus subtilis / Q000235:genetics*; D004260:DNA Repair; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial; D009675:Novobiocin / Q000494:pharmacology; D010957:Plasmids; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D013014:SOS Response, Genetics; D059005:Topoisomerase II Inhibitors; D014158:Transcription, Genetic
D012329:RNA, Bacterial; D012333:RNA, Messenger; D059005:Topoisomerase II Inhibitors; D009675:Novobiocin