Cross-linking of VLA/CD29 molecule has a co-mitogenic effect with anti-CD3 on CD4 cell activation in serum-free culture system.
In this study, we demonstrated that antibodies reactive with a common beta 1 subunit (CD29) of the VLA family of antigens could synergize with CD3 but not CD2 in inducing CD4 cell proliferation in a serum-free culture system. The extent of activation was similar to that obtained when CD4 cells were cultured with anti-CD3 plus fibronectin. The proliferative response induced via the CD3-CD29 pathway was associated with the interleukin (IL) 2 autocrine pathway, including IL 2 production and IL 2 receptor expression. Specifically, cross-linking of VLA-5, but not other members of the VLA family of antigens with the CD3 molecule induced strong CD4 cell activation. However, cross-linking of CD3 with other cell surface antigens such as CD4 did not induce CD4 cell activation in this serum-free culture system. The above results strongly suggest that the VLA/CD29 family of antigens may play an important role in regulating CD4 T cell activation via the CD3-T cell receptor pathway.
Yamada
A
A
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115.
Nojima
Y
Y
Sugita
K
K
Dang
N H
NH
Schlossman
S F
SF
Morimoto
C
C
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000502:physiology*; D000943:Antigens, Differentiation / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D018801:CD2 Antigens; D017252:CD3 Complex; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D003470:Culture Media; D005353:Fibronectins / Q000502:physiology; D006801:Humans; D019012:Integrin beta1; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000502:physiology; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D016029:Receptors, Very Late Antigen / Q000502:physiology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D017252:CD3 Complex; D003470:Culture Media; D005353:Fibronectins; D019012:Integrin beta1; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D016029:Receptors, Very Late Antigen