Detection of proteins related to a salivary glycoprotein (EP-GP). Concentrations in human secretions (saliva, sweat, tears, nasal mucus, cerumen, seminal plasma).
With a highly specific monoclonal antibody against a previously isolated and characterized human salivary 19-20-kDa glycoprotein, designated as extra-parotid glycoprotein [Rathman et al. (1989) J. Biol. Buccale 17, 199-208], a common epitope was detected on proteins in several excretory human body fluids. With a quantitative ELISA the EP-GP epitope was measured in widely different concentrations in several secretory human body fluids in the descending order of seminal plasma much greater than tears approximately nasal mucus approximately sweat much greater than saliva. Crossreactivity was also observed in cerumen but not in milk, cerebrospinal fluid, blood plasma and urine. The relative amount of EP-GP in the positively reacting secretions was however, in the same order in each fluid per mg of protein on an average of 1% of the total protein amount. The EP-GP-epitope bearing proteins found in the various human secretions were further characterized by means of electrophoresis and immunoblotting. The molecular masses and the isoelectric points of the proteins in the different secretions display strong resemblance to values found for the salivary glycoprotein EP-GP (molecular masses 19 and 20 kDa; pI values between 4.8 and 5.4). All these findings point to the presence of proteins related to EP-GP in human secretions other than saliva.
Schenkels
L C
LC
Department of Oral Biochemistry, Vrije Universiteit, Amsterdam.
Rathman
W M
WM
Veerman
E C
EC
Nieuw Amerongen
A V
AV
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D001826:Body Fluids / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009093:Mucus / Q000737:chemistry; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D012661:Semen / Q000737:chemistry; D013542:Sweat / Q000737:chemistry; D013666:Tears / Q000737:chemistry
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D012471:Salivary Proteins and Peptides