title: Cryoprecipitate of intermediate purity produced in a closed thaw-siphon system from DDAVP stimulated blood donor plasma.
abstract: The effects of intravenous administration of DDAVP to blood donors and the use of DDAVP plasma for the production of cryoprecipitate in the closed thaw-siphon system were evaluated. DDAVP treatment produced on the average a 3.2-fold rise in plasma levels of factor VIII. Von Willebrand factor antigen increased to a lesser extent. Cryoprecipitate prepared from 220-280 ml aliquots of DDAVP stimulated donor plasma contained 472 +/- 210 units of factor VIII and 276 +/- 130 units of von Willebrand factor antigen. The average yield of factor VIII was 57% of that in the prefrozen plasma. The specific activity of factor VIII in cryoprecipitate was 0.77 +/- 0.44 U/mg protein, comparable to that for intermediate purity concentrates. Thus, by the use of DDAVP and the thaw-siphon technique it is possible to produce cryoprecipitate 4-7 times as potent as conventionally manufactured preparations.
authors.affiliation: Department of Blood Storage and Preparation, Institute of Haematology, Warsaw, Poland.
authors.forename: G
authors.initials: G
authors.lastname: Konecka
authors.forename: K
authors.initials: K
authors.lastname: Bykowska
authors.forename: A
authors.initials: A
authors.lastname: Ludwicka
authors.forename: M
authors.initials: M
authors.lastname: Letowska
authors.forename: Z
authors.initials: Z
authors.lastname: Wegrzynowicz
authors.forename: J
authors.initials: J
authors.lastname: Sablinski
authors.forename: S
authors.initials: S
authors.lastname: Lopaciuk
mesh_terms: D001782:Blood Donors; D011232:Chemical Precipitation; D003894:Deamino Arginine Vasopressin / Q000008:administration & dosage* / Q000494:pharmacology; D005169:Factor VIII / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D005615:Freezing; D006801:Humans; D008297:Male; D012016:Reference Values; D014841:von Willebrand Factor / Q000302:isolation & purification
chemical_list: D014841:von Willebrand Factor; D005169:Factor VIII; D003894:Deamino Arginine Vasopressin