Functional expression of the alpha 1 subunit of the AMPA-selective glutamate receptor channel, using a baculovirus system.
Using a baculovirus expression vector system, the alpha 1 subunit of the mouse AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-selective glutamate receptor channel was expressed in insect Spdoptera frugiperda cells. Binding studies using [3H]AMPA showed that insect cells infected with the recombinant virus expressed approximately 1.8 x 10(5) binding sites per cell on their surface. The ligand binding characteristics of the receptors expressed in insect cells were examined. The baculovirus-insect cell expression system affords high-efficiency expression of the receptor in sufficient amounts to permit structural and functional analyses.
Kawamoto
S
S
Department of Bacteriology, Yokohama City, University School of Medicine, Japan.
Onishi
H
H
Hattori
S
S
Miyagi
Y
Y
Amaya
Y
Y
Mishina
M
M
Okuda
K
K
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D004274:DNA, Recombinant; D015870:Gene Expression; D005822:Genetic Vectors; D005971:Glutamates / Q000378:metabolism / Q000494:pharmacology; D018698:Glutamic Acid; D007051:Ibotenic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D007313:Insecta / Q000235:genetics / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D007608:Kainic Acid / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D016318:Quisqualic Acid / Q000494:pharmacology; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
D004274:DNA, Recombinant; D005971:Glutamates; D007473:Ion Channels; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D007051:Ibotenic Acid; D018698:Glutamic Acid; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D016318:Quisqualic Acid; D007608:Kainic Acid