Properties of bovine nephritogenic antigen that induces anti-GBM nephritis in rats and its similarity to the Goodpasture antigen.
The nephritogenic antigen that induces antiglomerular basement membrane antibody-induced glomerulonephritis (anti-GBM nephritis) in rats was isolated from collagenase-solubilized bovine renal basement membranes. Purification was achieved using antibody-coupled affinity columns which were originally used for the purification of trypsin-solubilized nephritogenic antigen (Sado et al. 1984a). The nephritogenic antigen was a heteropolymer composed of P2 (Mr 28 kDa) and P3 (Mr 30 kDa) polypeptides as monomers and their dimers in sodium-dodecyl-sulfate (SDS) polyacrylamide gel electrophoresis. The P3 polypeptide was considered to be the nephritogenic epitope, since a fraction composed of the P2 polypeptide alone was not nephritogenic. The properties of the nephritogenic epitope were the same as those of the Goodpasture epitope (M2*), which is a noncollagenous domain of the alpha 3 chain of type IV collagen (Butkowski et al. 1985; Saus et al. 1988), indicating that the nephritogenic antigen is the same as the Goodpasture antigen.
Sado
Y
Y
Division of Immunology, Shigei Medical Research Institute, Okayama, Japan.
Kagawa
M
M
Naito
I
I
Okigaki
T
T
D000818:Animals; D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology; D000906:Antibodies / Q000276:immunology; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000139:chemically induced / Q000276:immunology; D001485:Basement Membrane / Q000276:immunology; D002417:Cattle; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003094:Collagen / Q000276:immunology*; D024141:Collagen Type IV; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology; D005260:Female; D005921:Glomerulonephritis / Q000139:chemically induced / Q000276:immunology; D026541:Heymann Nephritis Antigenic Complex; D006801:Humans; D005779:Immunodiffusion; D007678:Kidney Glomerulus / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology / Q000302:isolation & purification* / Q000633:toxicity; D003012:Microbial Collagenase; D051381:Rats; D011921:Rats, Inbred WKY; D014357:Trypsin
D000906:Antibodies; D001324:Autoantigens; D024141:Collagen Type IV; D000939:Epitopes; D026541:Heymann Nephritis Antigenic Complex; D008562:Membrane Glycoproteins; C057112:type IV collagen alpha3 chain; D003094:Collagen; D014357:Trypsin; D003012:Microbial Collagenase