title: Expression and function of a variant T cell receptor complex lacking CD3-gamma.
abstract: A T cell line termed DIL2 has been derived from an infant with a polyclonal T cell receptor (TCR)/CD3 cell surface expression defect. Indirect immunofluorescence showed that the expression of certain TCR/CD3 epitopes (like those detected by WT31 and BMA031 monoclonals) was strongly reduced (around five-fold) on DIL2, whereas other epitopes (like those detected by SP34 and Leu4) were only around two-fold lower than in normal T cell lines. Specific immunoprecipitates of surface-radioiodinated DIL2 cells contained TCR-alpha, TCR-beta, CD3-delta, CD3-epsilon and TCR-zeta chains, but lacked CD3-gamma. This structural TCR/CD3 variant was, however, capable of transducing certain activation signals, since normal proliferation and a low but significant calcium flux was observed in DIL2 cells after engagement with specific antibodies. Our data suggest that a functional TCR/CD3 complex can be expressed on the surface of T cells in the absence of CD3-gamma.
authors.affiliation: Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Pérez-Aciego
authors.forename: B
authors.initials: B
authors.lastname: Alarcón
authors.forename: A
authors.initials: A
authors.lastname: Arnaiz-Villena
authors.forename: C
authors.initials: C
authors.lastname: Terhorst
authors.forename: M
authors.initials: M
authors.lastname: Timón
authors.forename: O G
authors.initials: OG
authors.lastname: Segurado
authors.forename: J R
authors.initials: JR
authors.lastname: Regueiro
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000172:deficiency* / Q000276:immunology; D015152:Blotting, Northern; D017252:CD3 Complex; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism; D011863:Radioimmunoassay; D011948:Receptors, Antigen, T-Cell / Q000172:deficiency* / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D000939:Epitopes; D007376:Interleukin-2; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta; D012313:RNA; D002118:Calcium