DNA ploidy studies of benign and malignant tumours: comparison of flow cytometry and image analysis techniques using two types of cytological specimen.
DNA ploidy studies were carried out on Feulgen stained smears and cytocentrifuge preparations from 35 malignant tumours and four benign neoplasms using the CAS image analyser. The smears were prepared from scrapings from fresh tumour tissue whereas the cytocentrifuge preparations were prepared from single nuclear suspensions from paraffin-embedded cell blocks from the same tumour. Histograms obtained by image analysis of the tumour scrapes were compared with those obtained on the cytocentrifuge preparations. Concordant results were obtained in four benign tumours (100%) and 32 malignant tumours (91%). The results obtained by image analysis were also compared with results obtained by flow cytometry of the tumour tissue. Discordant results were obtained for three malignant tumours. Possible reasons for the discrepancy include sampling error, tumour heterogeneity and selective loss of cell populations during processing.
Cohen
C
C
Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30322.
Tickman
R J
RJ
DeRose
P B
PB
Whitaker
B P
BP
D001483:Base Sequence; D002138:Calibration; D002467:Cell Nucleus / Q000473:pathology; D002498:Centrifugation / Q000379:methods; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*; D011003:Ploidies; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling
D004273:DNA, Neoplasm