Mechanism of ethanol-induced aggregation in whole blood.
Effects of ethanol on blood clotting and platelet aggregation have been reported in many models, but its in vitro actions in whole blood, impedance aggregometry have not been reported. We investigated the effect of ethanol in vitro in whole blood and platelet rich plasma of humans and rats, as measured by impedance aggregometry. Ethanol (34 to 170 mM) induced concentration-dependent aggregation in whole blood but not platelet rich plasma. In further studies in rats, aggregation was inhibited by pretreatment of whole blood with the prostacyclin analog iloprost or the enzyme apyrase, which degrades ADP to AMP. Levels of ethanol which produced aggregation in whole blood were also associated with concentration-dependent hemolysis. Based on the requirement for whole blood for ethanol-induced aggregation, the inhibitory effect of apyrase and our observation of hemolysis, and previous studies which have demonstrated the potential contribution of ADP from lysed red blood cells to platelet aggregation, we conclude that ethanol-induced aggregation in whole blood is mediated by erythrocyte lysis and the ADP released from these cells.
Abi-Younes
S A
SA
Department of Physiology & Biophysics, Georgetown University Medical Center, Washington, DC 20007.
Ayers
M L
ML
Myers
A K
AK
D000244:Adenosine Diphosphate / Q000037:antagonists & inhibitors; D000818:Animals; D001081:Apyrase / Q000494:pharmacology; D003094:Collagen / Q000037:antagonists & inhibitors; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D016285:Iloprost / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D010975:Platelet Aggregation Inhibitors; D000431:Ethanol; D000244:Adenosine Diphosphate; D003094:Collagen; D001081:Apyrase; D016285:Iloprost