Intracellular antigenic changes associated with meiosis in the orthopteran Stauroderus scalaris.
Monoclonal antibodies have been prepared against purified pachytene cells from grasshopper testes. Immunoblotting and immunofluorescence analyses identified those monoclonal antibodies which showed specificity for antigens in pachytene cells. Several antigenic changes were found to be associated with meiotic cells. Five monoclonal antibodies detected antigens which were located in the cytoplasm of premeiotic cells but were nuclear during meiosis. One monoclonal antibody showed a discrete cytoplasmic fluorescent pattern in meiotic, but not in premeiotic, cells. Another bound specifically to the nuclei of some epithelial cells at the base of follicles in mature testes.
Percy
M J
MJ
Division of Genetic Engineering, School of Biology and Biochemistry, Queen's University of Belfast, Northern Ireland.
Fletcher
H L
HL
Trudgett
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000378:metabolism*; D002467:Cell Nucleus / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006110:Grasshoppers; D015151:Immunoblotting; D008297:Male; D008540:Meiosis; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D013094:Spermatozoa / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes