Retrograde neuronal labelling by E. coli enterotoxin subunit B.
The present communication reports that subunit B of Escherichia coli heat-labile enterotoxin (LTB) can be utilized as a powerful tracer in neuroanatomical studies. LTB was injected into the limb muscle of the chick, or into the superior cervical ganglion of the rat. Sections were processed with an immunohistochemical technique using an antibody against LTB. After the LTB injection into the muscle, retrogradely labelled motoneurons were found: the entire extent of dendritic arborizations appeared labeled. After the LTB injection into the ganglion, virtually all of the preganglionic neurons were retrogradely labelled in the rat spinal cord.
Okado
N
N
Department of Anatomy, University of Tsukuba, Ibaraki, Japan.
Hayashi
H
H
Hosoya
Y
Y
Matsukawa
M
M
D000344:Afferent Pathways / Q000033:anatomy & histology*; D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000648:ultrastructure; D001427:Bacterial Toxins; D002645:Chickens; D004768:Enterotoxins; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005728:Ganglia, Sympathetic / Q000033:anatomy & histology*; D009132:Muscles / Q000294:innervation*; D009474:Neurons / Q000166:cytology*; D051381:Rats; D013116:Spinal Cord / Q000033:anatomy & histology*
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli