Absence of the 40-kDa form of (2'-5')oligoadenylate synthetase and its corresponding mRNA from skin fibroblasts derived from Alzheimer disease patients.
Cultured skin fibroblasts derived from Alzheimer disease patients fail to express the 1.6-kilobase (kb) mRNA and the corresponding 40-kDa form of (2'-5')oligoadenylate (2-5A) synthetase when exposed to interferon. In addition, the 3.6-kb mRNA, which is present in normal fibroblasts, is barely detectable in the Alzheimer disease counterpart. The deficiency of the 2-5A synthetase 1.6-kb mRNA and its corresponding protein is not related to an impairment of interferon receptors but most probably represents an alteration in the expression of the 2-5A synthetase gene. The data have potential implications for the diagnosis of Alzheimer disease and demonstrate that the absence of a specific form of 2-5A synthetase is linked to a disease state.
Khanna
K K
KK
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
An
S J
SJ
Wu
J M
JM
Landolfo
S
S
Hovanessian
A G
AG
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics / Q000378:metabolism*; D000544:Alzheimer Disease / Q000201:enzymology*; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D004790:Enzyme Induction / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007372:Interferons / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000378:metabolism; D017471:Receptors, Interferon; D012867:Skin / Q000166:cytology / Q000201:enzymology
D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase