Some substances with proposed digitalis-like effects evaluated on platelet functions sensitive for cardiac glycosides.
1. The effects of progesterone, corticosterone 21-acetate, chlormadinone acetate, dehydroepiandrosterone 3-sulfate and lysophosphatidylcholine were tested on 86Rb-uptake, 3H-5-HT-uptake, ADP-induced aggregation and 5-HT-induced shape change in human platelets. Ouabain and digoxin were used for reference. 2. Ouabain and digoxin 10(-5) M inhibited 86Rb-uptake by more than 85%, and chlormadinone acetate 10(-5) M by 20%. The other substances had no effects. 3. Ouabain and digoxin were potent inhibitors on 3H-5-HT-uptake, whereas chlormadinone acetate had no effect. 4. Ouabain and digoxin increased ADP-induced aggregation but chlormadinone acetate decreased it. 5-HT-induced shape change was decreased by ouabain and digoxin, and to a lesser extent by chlormadinone acetate and its vehicle (ethanol 1.0%).
Andersson
T L
TL
Department of Clinical Pharmacology, Lund University Hospital, Sweden.
Zygmunt
P
P
Vinge
E
E
D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002301:Cardiac Glycosides / Q000494:pharmacology*; D002715:Chlormadinone Acetate / Q000494:pharmacology; D003345:Corticosterone / Q000031:analogs & derivatives / Q000494:pharmacology; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives / Q000494:pharmacology; D019314:Dehydroepiandrosterone Sulfate; D004070:Digitalis; D004077:Digoxin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D010946:Plants, Medicinal; D010947:Plants, Toxic; D010974:Platelet Aggregation / Q000187:drug effects; D011374:Progesterone / Q000494:pharmacology; D012413:Rubidium / Q000493:pharmacokinetics; D012701:Serotonin / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D002301:Cardiac Glycosides; D008244:Lysophosphatidylcholines; D002715:Chlormadinone Acetate; D012701:Serotonin; D003687:Dehydroepiandrosterone; D011374:Progesterone; D019314:Dehydroepiandrosterone Sulfate; D010042:Ouabain; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase; C029939:corticosterone acetate; D012413:Rubidium; D003345:Corticosterone