title: Influence of internucleotide phosphate linkage on relative base stacking in 3'-5' and 2'-5' RNA: a circular dichroic spectroscopic study of RNA hexamer AACCUU.
abstract: The variations in base stacking interactions of two isomeric RNA hexamers, 3'-5'r (AACCUU) and 2'-5'r' (AACCUU), have been studied using temperature dependent CD spectroscopy. Both RNA hexamers, in single strand form, exhibited a right handed helical sense. Van't Hoff analysis of the CD spectral results, derived from a two state model, gave a higher enthalpy of stacking for 3'-5' RNA than for 2'-5'RNA. The results suggest that 3'-5' linkage in RNA facilitates formation of better helical stacks in relation to an isomeric 2'-5' linkage.
authors.affiliation: Division of Organic Chemistry, National Chemical Laboratory, Pune, India.
authors.forename: V
authors.initials: V
authors.lastname: Gopalakrishnan
authors.forename: R S
authors.initials: RS
authors.lastname: Ghadage
authors.forename: K N
authors.initials: KN
authors.lastname: Ganesh
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D007536:Isomerism; D009691:Nucleic Acid Denaturation; D009843:Oligoribonucleotides / Q000737:chemistry*; D010710:Phosphates; D012313:RNA / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D009843:Oligoribonucleotides; D010710:Phosphates; D012313:RNA