Purification of autoantibodies to myelin basic protein by antigen specific affinity chromatography from cerebrospinal fluid IgG of multiple sclerosis patients. Immunoreactivity studies with human myelin basic protein.
Immunoglobulin G (IgG) was purified by single-step protein A-Sepharose (Pharmacia) affinity chromatography from the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients and controls. Autoantibodies to myelin basic protein (anti-MBP) were isolated from the purified IgG fraction by two-step antigen specific affinity chromatography. Anti-MBP in the context of whole CSF or in purified form reacts equally to MBP prepared from non-MS or MS brain tissue. Kinetic studies of anti-MBP titers demonstrate that when anti-MBP is reacted with increasing amounts of non-MS or MS MBP, the autoantibody is immunoabsorbed by either antigen in vitro. Immunoabsorption of anti-MBP by MBP or its synthetic peptides may also be possible in vivo as a potential therapeutic tool.
Warren
K G
KG
Department of Medicine (Neurology), University of Alberta, Edmonton, Canada.
Catz
I
I
D001323:Autoantibodies / Q000302:isolation & purification*; D001921:Brain / Q000276:immunology; D002846:Chromatography, Affinity; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid* / Q000302:isolation & purification; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000276:immunology*; D004676:Myelin Basic Protein / Q000276:immunology* / Q000302:isolation & purification; D009902:Optic Neuritis / Q000134:cerebrospinal fluid / Q000276:immunology
D001323:Autoantibodies; D007074:Immunoglobulin G; D004676:Myelin Basic Protein