Comparison between synthetic nuclear localization signal peptides from the steroid/thyroid hormone receptors superfamily.
The main objective of the study is to demonstrate that short basic peptides from the steroid/thyroid hormone receptors superfamily act as Nuclear Localization Signals out of receptors context. Such synthesized peptides, chemically coupled to Bovine Serum Albumin, were shown to enable the corresponding BSA-conjugate to be transported to the nucleus. A second objective is to demonstrate the utility of viral cointernalization as a good method for rapid quantitation, comparison and competition in nuclear entry.
Hamy
F
F
INSERM Unité N. 16, Lille, France.
Helbecque
N
N
Henichart
J P
JP
D000256:Adenoviridae / Q000235:genetics; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000502:physiology*; D006801:Humans; D007624:KB Cells; D007700:Kinetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D009842:Oligopeptides / Q000138:chemical synthesis; D021382:Protein Sorting Signals / Q000235:genetics*; D011987:Receptors, Steroid / Q000235:genetics*; D011988:Receptors, Thyroid Hormone / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D002352:Carrier Proteins; D009842:Oligopeptides; D021382:Protein Sorting Signals; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone