Selective cytotoxic effects of immunotoxin--monoclonal anti-AFP-abrin-A chain conjugate on several human hepatoma cell lines.
A highly specific monoclonal antibody (anti-AFP) against alpha-fetoprotein (AFP) was linked to N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) to form conjugates which were purified with a protein A-sepharose CL-4B affinity column. The conjugate, PDP-Anti-AFP was then covalently coupled to the toxic abrin-A chain to synthesize immunotoxins. The immunotoxin, anti-AFP-abrin-A conjugate, which was also purified with a protein A-sepharose CL-4B affinity column, had a molecular weight of 180,000 and had 80% antigen-binding activity that of anti-AFP activity and 92% toxicity of abrin-A chain. The immunotoxin showed selective cytotoxicities toward the AFP secreting human hepatoma cell lines, such HepG2 and Hep3B, but not toward AFP non-secreting human hepatoma cell line, PLC/PRF/5.
Chow
L P
LP
Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, R.O.C.
Chang
K H
KH
Lin
J Y
JY
D000036:Abrin / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D006528:Carcinoma, Hepatocellular; D002455:Cell Division; D002470:Cell Survival; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D000922:Immunotoxins / Q000494:pharmacology*; D014176:Protein Biosynthesis; D013388:Succinimides; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D000922:Immunotoxins; D013388:Succinimides; D000509:alpha-Fetoproteins; D000036:Abrin; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate