title: A peptide-hormone-inactivating endopeptidase in Xenopus laevis skin secretion.
abstract: An endopeptidase was isolated from Xenopus laevis skin secretions. This enzyme, which has an apparent molecular mass of 100 kDa, performs a selective cleavage at the Xaa-Phe, Xaa-Leu, or Xaa-Ile bond (Xaa = Ser, Phe, Tyr, His, or Gly) of a number of peptide hormones, including atrial natriuretic factor, substance P, angiotensin II, bradykinin, somatostatin, neuromedins B and C, and litorin. The peptidase exhibited optimal activity at pH 7.5 and a Km in the micromolar range. No cleavage was produced in vasopressin, ocytocin, minigastrin I, and [Leu5]enkephalin, which include in their sequence an Xaa-Phe, Xaa-Leu, or Xaa-Ile motif. The endopeptidase activity was inhibited by divalent cation chelators and by phosphoramidon only at high concentrations (IC50 = 50 microM), whereas it was insensitive to classical inhibitors of chymotrypsin, angiotensin convertase, and serine and cysteine peptidases, as well as carboxypeptidases. It is hypothesized that this enzyme, which is distinct from neutral endopeptidase (EC 3.4.24.11), constitutes the prototype of a family of related metalloendopeptidases that inactivate peptide substrates by cleavage at the Xaa-Phe, Xaa-Leu, or Xaa-Ile bond.
authors.affiliation: Université Pierre et Marie Curie, Centre National de la Recherche Scientifique, Paris, France.
authors.forename: K M
authors.initials: KM
authors.lastname: Carvalho
authors.forename: C
authors.initials: C
authors.lastname: Joudiou
authors.forename: H
authors.initials: H
authors.lastname: Boussetta
authors.forename: A M
authors.initials: AM
authors.lastname: Leseney
authors.forename: P
authors.initials: P
authors.lastname: Cohen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006728:Hormones / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008666:Metalloendopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D012867:Skin / Q000201:enzymology; D013379:Substrate Specificity; D014982:Xenopus laevis / Q000502:physiology*
chemical_list: D006728:Hormones; D010455:Peptides; D011480:Protease Inhibitors; D008666:Metalloendopeptidases; C081597:peptide hormone inactivating endopeptidase