title: High-performance liquid chromatographic determination of bleomycins.
abstract: A rapid and specific ion-pair reversed-phase high-performance liquid chromatographic method was developed for the determination of bleomycins. The use of 5-microns particles of less adsorptive reversed-phase packings and sodium perchlorate as ion-pairing reagent permitted a short analysis time and the transferability of the separations on different batches of the reversed-phase materials. The detection sensitivity and precision of the method demonstrated that the system is suitable for routine analysis.
authors.affiliation: Universität Tübingen, Biologisches Institut, LB Mikrobiologie/Antibiotika, F.R.G.
authors.forename: H P
authors.initials: HP
authors.lastname: Fiedler
authors.forename: J
authors.initials: J
authors.lastname: Wachter
mesh_terms: D001761:Bleomycin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001761:Bleomycin