[Partial purification of glycosyltransferases participating in the biosynthesis of Salmonella anatum and S. kentucky O-antigens using high performance gel chromatography].
The solubilized glycosyltransferases which catalyse the biosynthesis of Salmonella anatum and S. kentucky O-specific polysaccharides were partially purified by HPLC on Superose 12. Two mannosyl transferases from S. kentucky were separated by gel chromatography; these transferases were found useful for chemical-enzymic synthesis of polyprenylpyrophosphate derivatives of trisaccharides Tal-Man-Gal and Man-Tal-Gal.
Druzhinina
T N
TN
Sizova
O V
OV
Torgov
V I
VI
Shibaev
V N
VN
D000942:Antigens, Bacterial / Q000096:biosynthesis*; D002850:Chromatography, Gel; D005700:Galactosyltransferases / Q000378:metabolism; D005964:Glucosyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D008364:Mannosyltransferases / Q000378:metabolism; D019081:O Antigens; D012475:Salmonella / Q000201:enzymology / Q000276:immunology*; D013045:Species Specificity
D000942:Antigens, Bacterial; D019081:O Antigens; D005700:Galactosyltransferases; D005964:Glucosyltransferases; D008364:Mannosyltransferases
Chastichnaia ochistka glikoziltransferaz, uchastvuiushchikh v biosinteze O-antigenov Salmonella antatum i S. kentucky s pomoshch'iu vysokoéffektivnoĭ gel'-khromatografii.