Mitotic spindle damage induced by 2-chlorobenzylidene malonitrile (CS) in V79 Chinese hamster cells examined by differential staining of the spindle apparatus and chromosomes.
A 3-h exposure of V79 Chinese hamster cells with the sensory irritant 2-chlorobenzylidene malonitrile (CS) caused apolar mitoses in a dose-dependent manner. With a preparation and staining technique that allows for the visualization of the spindle apparatus and the chromosomes it was found that unlike in Colcemid-induced c-metaphases residual spindle fibers or microtubule material were present in the majority of CS-induced c-metaphases. The observation suggests different mechanisms for the induction of the c-mitotic effect by the two spindle poisons.
Salassidis
K
K
GSF-Forschungszentrum für Umwelt und Gesudheit, Institut für Strahlenbiologie, München-Neuherberg, F.R.G.
Schmid
E
E
Bauchinger
M
M
D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D008677:Metaphase; D009152:Mutagenicity Tests; D008941:Spindle Apparatus / Q000187:drug effects* / Q000648:ultrastructure; D013194:Staining and Labeling; D009763:o-Chlorobenzylidenemalonitrile / Q000633:toxicity*
D009763:o-Chlorobenzylidenemalonitrile