The complex of alpha 2-macroglobulin with CD2 in the plasma of gastric carcinoma patients.
alpha 2-macroglobulin (alpha 2M) preparations purified from the plasma of healthy persons and gastric carcinoma patients were further analysed by immunochemical techniques. It was found that only alpha 2M from the plasma of gastric carcinoma patients gave a positive reaction with E-receptor sheep antiserum in rocket immunoelectrophoresis. The alpha 2M fraction giving the positive reaction with E-receptor sheep antiserum was isolated and analysed by the immunoblotting technique with monoclonal antibodies to T11 antigen. The presence of the alpha 2M-CD2 complex in the plasma of gastric carcinoma patients was demonstrated. This finding may explain the immunosuppressive effect of the plasma alpha 2M in cancer patients.
Smorodin
E P
EP
Department of Clinical Oncology, Institute of Experimental and Clinical Medicine, Estonia, USSR.
Kurtenkoy
O A
OA
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D018801:CD2 Antigens; D002277:Carcinoma / Q000276:immunology*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D011961:Receptors, Fc / Q000276:immunology; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000276:immunology*; D013274:Stomach Neoplasms / Q000276:immunology*; D000511:alpha-Macroglobulins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D011961:Receptors, Fc; D017455:Receptors, IgE; D011971:Receptors, Immunologic; D000511:alpha-Macroglobulins