Two-parameter mobile phase optimization for the simultaneous high-performance liquid chromatographic determination of dopamine, serotonin and related compounds in microdissected rat brain nuclei.
A new high-pressure liquid chromatography method with electrochemical detection is described that allows the simultaneous determination of dopamine, serotonin, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylacetic acid, 5-hydroxytryptophan and 5-hydroxyindoleacetic acid in microdissected nuclei from individual rat brains. No sample pre-treatment steps are required. Resolution and analysis time were optimized by a simple limited optimization procedure, involving two-parameter factorial design.
Lema
M
M
Department of Physiology, Faculty of Biology, University of Santiago, Galicia, Spain.
Otero
J
J
Marcó
J
J
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D006916:5-Hydroxytryptophan / Q000032:analysis; D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000032:analysis*; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis*
D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D008734:Methoxyhydroxyphenylglycol; D006897:Hydroxyindoleacetic Acid; D006916:5-Hydroxytryptophan; D004298:Dopamine; D009638:Norepinephrine