title: Genetic analysis in cystic fibrosis using the amplification refractory mutation system (ARMS): the J3.11 MspI polymorphism.
abstract: A new method of genetic analysis has been devised. The method, amplification refractory mutation system (ARMS), has been used to genotype the J3.11 MspI restriction fragment length polymorphism (RFLP) closely linked to cystic fibrosis (CF). The DNA sequence for both alleles of this dimorphism has been used to design ARMS primers. These allow genotyping of DNA isolated from blood, Guthrie cards, and buccal cells.
authors.affiliation: ICI Pharmaceuticals, Biotechnology Department, Mereside, Alderley Park, Macclesfield, Cheshire.
authors.forename: C R
authors.initials: CR
authors.lastname: Newton
authors.forename: C
authors.initials: C
authors.lastname: Summers
authors.forename: L E
authors.initials: LE
authors.lastname: Heptinstall
authors.forename: J R
authors.initials: JR
authors.lastname: Lynch
authors.forename: R S
authors.initials: RS
authors.lastname: Finniear
authors.forename: D
authors.initials: D
authors.lastname: Ogilvie
authors.forename: J C
authors.initials: JC
authors.lastname: Smith
authors.forename: A F
authors.initials: AF
authors.lastname: Markham
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002610:Cheek / Q000033:anatomy & histology; D003550:Cystic Fibrosis / Q000235:genetics*; D004247:DNA / Q000097:blood / Q000737:chemistry; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005784:Gene Amplification; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D010375:Pedigree; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004247:DNA; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific