Endothelin-1 stimulates hypertrophy and contractility of neonatal rat cardiac myocytes in a serum-free medium. II.
The effect of endothelin-1 (ET-1) on rat cardiac myocytes cultured in a serum-free medium was determined. Cardiac myocytes cultured in our medium showed an increased rate of protein synthesis, morphological size, contraction rate, and Ca2+ uptake when ET-1 was added. These actions of ET-1 were inhibited by a protein kinase C inhibitor, H-7. Among these four phenomena, contraction and Ca2+ uptake were inhibited by a Ca2+ channel blocker, nicardipine. Therefore, it is likely that the signal for ET-1 to induce these phenomena is transduced by kinase C, and Ca2+ uptake is related to maintaining a high contraction rate.
Suzuki
T
T
Bio-Science Laboratory, Inc., Yamagata, Japan.
Hoshi
H
H
Sasaki
H
H
Mitsui
Y
Y
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D003470:Culture Media; D016232:Endothelins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D006984:Hypertrophy; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis; D009200:Myocardial Contraction / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D009529:Nicardipine / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D003470:Culture Media; D016232:Endothelins; D007546:Isoquinolines; D009124:Muscle Proteins; D010879:Piperazines; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D009529:Nicardipine; D011493:Protein Kinase C; D002118:Calcium