Drastic change of reverse micellar structure by protein or enzyme addition.
On addition of cytochrome c to a AOT reverse micellar solution, the percolation process usually observed at high temperatures and surfactant concentrations, occurs at room temperature. This is observed either at relatively high water content at a given cytochrome c concentration or at low content on increasing the cytochrome c concentration. On increasing the water content a phase transition is observed with two optically transparent phases. A similar phase transition is observed on solubilizing various enzymes. The temperature of the transition appears to be strongly dependent on the location of the macromolecule in the reserve micelle.
Huruguen
J P
JP
Université Pierre et Marie Curie, Laboratoire S.R.I., bâtiment de Chimie-Physique, Paris, France.
Pileni
M P
MP
D002918:Chymotrypsin / Q000737:chemistry; D003574:Cytochrome c Group / Q000737:chemistry; D004143:Dioctyl Sulfosuccinic Acid; D004553:Electric Conductivity; D008027:Light; D046911:Macromolecular Substances; D008823:Micelles; D015394:Molecular Structure; D009795:Octanes; D012260:Ribonucleases / Q000737:chemistry; D012542:Scattering, Radiation; D012995:Solubility; D013054:Spectrophotometry, Atomic; D013696:Temperature; D014867:Water
D003574:Cytochrome c Group; D046911:Macromolecular Substances; D008823:Micelles; D009795:Octanes; D014867:Water; D004143:Dioctyl Sulfosuccinic Acid; D012260:Ribonucleases; D002918:Chymotrypsin; C045798:2,2,4-trimethylpentane