Lymphotoxin and tumor necrosis factor-alpha production by myelin basic protein-specific T cell clones correlates with encephalitogenicity.
Lymphokine activity in seven myelin basic protein (MBP)-specific T cell clones was examined. All of the clones recognize MBP peptide 1-9 in the context of I-Au. A strong positive correlation was found between levels of lymphotoxin (LT) and tumor necrosis factor alpha (TNF-alpha) mRNA and biological activity on L929 cells and their capacity to induce paralysis, the clinical hallmark of experimental allergic encephalomyelitis (EAE). No correlation was found between interleukin-2 or gamma interferon production and encephalitogenicity. LT and/or TNF-alpha may play a central role in the pathogenesis of EAE.
Powell
M B
MB
Department of Neurology, Stanford University School of Medicine, CA 94305.
Mitchell
D
D
Lederman
J
J
Buckmeier
J
J
Zamvil
S S
SS
Graham
M
M
Ruddle
N H
NH
Steinman
L
L
D000818:Animals; D002999:Clone Cells / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology*; D008222:Lymphokines / Q000096:biosynthesis*; D008233:Lymphotoxin-alpha / Q000096:biosynthesis; D051379:Mice; D004676:Myelin Basic Protein / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008222:Lymphokines; D008233:Lymphotoxin-alpha; D004676:Myelin Basic Protein; D014409:Tumor Necrosis Factor-alpha