Epitope mapping of the Dermatophagoides pteronyssinus house dust mite major allergen Der p II using overlapping synthetic peptides.
Fourteen synthetic peptides of 15 amino acid residues length, overlapping by five residues and spanning the entire sequence of the major allergen Der p II from the house dust mite Dermatophagoides pteronyssinus were synthesized. These peptides were coupled to CNBr-activated Sepharose-4B and used as solid-phase antigens in epitope mapping studies using human IgE antisera. These antibodies bound predominantly to the peptide comprising residues 65-78, the binding of which was inhibited by native Der p II. In addition these antisera bound, to a lesser extent, to the peptide that comprised residues 1-15, which binding was not inhibited by native Der p II. Thus, we found one sequential epitope for a number of IgE sera.
van 't Hof
W
W
Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Driedijk
P C
PC
van den Berg
M
M
Beck-Sickinger
A G
AG
Jung
G
G
Aalberse
R C
RC
D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000937:Antigen-Antibody Reactions; D039741:Antigens, Dermatophagoides; D002874:Chromosome Mapping; D000939:Epitopes / Q000235:genetics*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008925:Mites / Q000276:immunology*; D008969:Molecular Sequence Data; D011852:Radioallergosorbent Test
D000485:Allergens; D039741:Antigens, Dermatophagoides; D000939:Epitopes; D007073:Immunoglobulin E