Activities of human acidic fibroblast growth factor in an in vitro dermal equivalent model.
Acidic fibroblast growth factor is a potent mitogen for human dermal fibroblasts in an in vitro three-dimensional collagen matrix, the "dermal equivalent." Both cell numbers and DNA synthesis are optimally stimulated by daily doses of 1 ng/ml of the pure human mitogen in the presence of heparin, which binds to, and stabilizes, the protein. Under daily treatment by 1 ng/ml aFGF, the fibroblast mitogenic response is marked but transient, and decreases steadily when fibroblasts mature in the collagen matrix. aFGF mitogenic stimulation also results in a decrease in cellular volume and inhibition of fibroblast-mediated contraction of the collagen gel. Various dosing regimes indicate that, although the greatest mitotic response was generated by daily dosing, nearly optimal responses can also be achieved with either a short duration of early daily dosing or longer-term intermittent treatment.
Dubertret
L
L
INSERM U 312, Hôpital Henri Mondor, Creteil, France.
Brunner-Ferber
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F
Misiti
J
J
Thomas
K A
KA
Dubertret
M L
ML
D002452:Cell Count / Q000187:drug effects; D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008954:Models, Biological; D012867:Skin / Q000737:chemistry*; D013997:Time Factors
D016220:Fibroblast Growth Factor 1