In vitro transcription from cauliflower mosaic virus promoters by a cell-free extract from tobacco cells.
We have studied transcription from the cauliflower mosaic virus 19S and 35S promoters in a cell-free system derived from tobacco cells in suspension culture. While a whole-cell extract is incapable of detectable transcription from these promoters, successive purification by column chromatography allows the preparation of two fractions which contain all factors necessary for transcription from the 19S promoter. In contrast, transcription from the 35S promoter leads to the accumulation of short RNAs. This accumulation can only be partially alleviated by modifying the conditions of transcription.
Cooke
R
R
Laboratoire de Physiologie Végétale (UA565 du CNRS), Université de Perpignan, France.
Penon
P
P
D001483:Base Sequence; D002474:Cell-Free System; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D010944:Plants / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D014158:Transcription, Genetic
D012313:RNA; D004247:DNA; D012319:RNA Polymerase II