Isolation and genetic study of p-fluoro-DL-phenylalanine-resistant mutants overproducing beta-phenethyl-alcohol in Saccharomyces cerevisiae.
p-Fluoro-DL-phenylalanine (PFP)-resistant mutants which produce a large amount of beta-phenethyl-alcohol, a rose-like flavor component, were isolated from the isogenic strains X2180-1A and X2180-1B of Saccharomyces cerevisiae. Cells of these mutants accumulated phenylalanine and tryptophan more than 3-fold times that of wild-type cells, while they accumulated less than half the tyrosine. The activity of prephenate dehydrogenase (PDG) (EC 1.3.1.12) was markedly decreased while that of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (EC 4.1.2.15) was increased. Genetic analysis revealed that the mutation occurred at the TYR1 locus, encoding PDG, and that the mutated TYR1 gene, try1-pfp, caused both PFP resistance and beta-phenethyl-alcohol overproduction. This was supported by molecular genetic studies with cloned tyr1-pfp DNA.
Fukuda
K
K
Foods and Liquors Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Ibaraki, Japan.
Watanabe
M
M
Asano
K
K
Ouchi
K
K
Takasawa
S
S
D000483:Alleles; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D004352:Drug Resistance, Microbial / Q000235:genetics; D005800:Genes, Fungal; D005808:Genes, Recessive; D008969:Molecular Sequence Data; D009154:Mutation; D010626:Phenylethyl Alcohol / Q000378:metabolism*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014170:Transformation, Genetic; D010135:p-Fluorophenylalanine / Q000494:pharmacology*
D004271:DNA, Fungal; D010135:p-Fluorophenylalanine; D010626:Phenylethyl Alcohol