Effects of indomethacin, triamcinolone, and dexamethasone on recombinant human interleukin-1-induced substance P and prostaglandin E2 levels in rabbit knee joints.
Intra-articular (i.a.) injection of recombinant human interleukin-1 alpha (rHuIL-1 alpha) in rabbit knees induced both an inflammation, as determined by increases in leukocytes in the joint fluid, and cartilage degradation, as measured by loss of proteoglycan. Substance P (SP) and prostaglandin E2 (PGE2) levels in the joint lavage are also elevated. Treatment with 5 mg indomethacin/kg, p.o., b.i.d., 2 mg triamcinolone i.a., and 10 mg dexamethasone/kg, p.o., reduced synovial lavage leukocyte counts, as well as PGE2 and SP lavage concentrations induced with IL-1 injection. However, none of the treatments inhibited rHuIL-1-induced proteoglycan loss.
O'Byrne
E M
EM
Research Department, CIBA-GEIGY Corporation, Summit, NJ 07901.
Blancuzzi
V
V
Wilson
D
D
Wong
M
M
Peppard
J
J
Simke
J P
JP
Jeng
A Y
AY
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis*; D006614:Hindlimb / Q000187:drug effects / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology*; D007270:Injections, Intra-Articular; D007375:Interleukin-1 / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D007596:Joints / Q000187:drug effects / Q000378:metabolism*; D007958:Leukocyte Count / Q000187:drug effects; D011509:Proteoglycans / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000494:pharmacology; D013373:Substance P / Q000096:biosynthesis*; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism; D014221:Triamcinolone / Q000494:pharmacology*
D007375:Interleukin-1; D007457:Iodine Radioisotopes; D011509:Proteoglycans; D011994:Recombinant Proteins; D014221:Triamcinolone; D013373:Substance P; D003907:Dexamethasone; D015232:Dinoprostone; D007213:Indomethacin