title: Spontaneously productive C-type retrovirus infection of fish cell lines.
abstract: The spontaneous production and release of morphologically typical, 85 to 90 nm diameter C-type retrovirus particles from four cell lines derived from three species of warmwater fish have been identified. Virus pellets from cell culture supernatants showed high levels of Mn(2+)-dependent reverse transcriptase activity at 24 degrees C. Peak enzyme activity was associated with a 1 x 16 g/ml sucrose gradient fraction. All four isolates induced a cytolytic infection of a bluegill fry cell line within 6 to 10 days.
authors.affiliation: Institute of Aquaculture, University of Stirling, U.K.
authors.forename: G N
authors.initials: GN
authors.lastname: Frerichs
authors.forename: D
authors.initials: D
authors.lastname: Morgan
authors.forename: D
authors.initials: D
authors.lastname: Hart
authors.forename: C
authors.initials: C
authors.lastname: Skerrow
authors.forename: R J
authors.initials: RJ
authors.lastname: Roberts
authors.forename: D E
authors.initials: DE
authors.lastname: Onions
mesh_terms: D000818:Animals; D002460:Cell Line; D005399:Fishes / Q000382:microbiology*; D010471:Perches / Q000382:microbiology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism; D014771:Virion / Q000378:metabolism
chemical_list: D012194:RNA-Directed DNA Polymerase