title: DNA histogram debris theory and compensation.
abstract: In this paper we describe a theory of DNA histogram debris generation and compensation that can be applied to paraffin-embedded frozen tissue preparations. The theory predicts the distribution of fragments generated from single and multiple random sectioning of three-dimensional ellipsoids representing nuclei. The fragment distribution is assumed to be a major component of the underlying debris in DNA histograms. A comparison of S-phase fractions (SPF) from matched tissue prepared by frozen and formalin-fixed paraffin-embedded DNA methods demonstrates the usefulness of the theory.
authors.affiliation: Maine Cytometry Research Institute, Verity Software House Inc., Portland.
authors.forename: C B
authors.initials: CB
authors.lastname: Bagwell
authors.forename: S W
authors.initials: SW
authors.lastname: Mayo
authors.forename: S D
authors.initials: SD
authors.lastname: Whetstone
authors.forename: S A
authors.initials: SA
authors.lastname: Hitchcox
authors.forename: D R
authors.initials: DR
authors.lastname: Baker
authors.forename: D J
authors.initials: DJ
authors.lastname: Herbert
authors.forename: D L
authors.initials: DL
authors.lastname: Weaver
authors.forename: M A
authors.initials: MA
authors.lastname: Jones
authors.forename: E J
authors.initials: EJ
authors.lastname: Lovett
mesh_terms: D002453:Cell Cycle; D003198:Computer Simulation; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry; D005629:Frozen Sections; D008954:Models, Biological; D009010:Monte Carlo Method; D010232:Paraffin; D013194:Staining and Labeling
chemical_list: D010232:Paraffin; D004247:DNA