title: 5-Azacytidine induction of a cellular heat shock protein and expression of the major immediate early protein of human cytomegalovirus in Vero cells.
abstract: Human cytomegalovirus can support an abortive infection in Vero cells and only some immediate early events are expressed in a low proportion of the cell population. It has been shown that cellular factors partially remove blocks in cells abortively or latently infected by HCMV, whereas 5-azacytidine is known to act on gene expression and cell differentiation. In this work we present evidence that the treatment of HCMV infected Vero cells with 5-azacytidine induces the expression of the major immediate early protein (68K) of Human cytomegalovirus by enhancing a 72K cellular heat shock protein.
authors.affiliation: Istituto di Microbiologia, Università di Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Zerbini
authors.forename: M
authors.initials: M
authors.lastname: Musiani
authors.forename: D
authors.initials: D
authors.lastname: Gibellini
authors.forename: G
authors.initials: G
authors.lastname: Gentilomi
authors.forename: M
authors.initials: M
authors.lastname: La Placa
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis*; D001374:Azacitidine / Q000494:pharmacology*; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D017874:Immediate-Early Proteins; D015151:Immunoblotting; D014709:Vero Cells
chemical_list: D000956:Antigens, Viral; D006360:Heat-Shock Proteins; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus; D001374:Azacitidine