Anatomical and electrophysiological evidence for a glycinergic inhibitory innervation of the rat locus coeruleus.
Using a highly specific antiserum to glycine and a very sensitive immunohistochemical technique with streptavidin-horseradish peroxidase, we visualized for the first time a dense plexus of glycine varicose fibers in the locus ceruleus (LC) of the rat. We further demonstrated that iontophoretically applied glycine inhibits the spontaneous LC noradrenergic cell discharge and that this inhibition is blocked by co-iontophoresis of strychnine. These anatomical and electrophysiological results indicate that the rat locus ceruleus receives an inhibitory glycinergic input.
Luppi
P H
PH
Département de Médecine Expérimentale, INSERM U52, CNRS URA 1195, Faculté de Médecine, Université Claude Bernard, Lyon, France.
Charlety
P J
PJ
Fort
P
P
Akaoka
H
H
Chouvet
G
G
Jouvet
M
M
D000818:Animals; D004594:Electrophysiology; D005998:Glycine / Q000502:physiology*; D007150:Immunohistochemistry; D008125:Locus Coeruleus / Q000166:cytology / Q000502:physiology; D009412:Nerve Fibers / Q000502:physiology; D009433:Neural Inhibition / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D005998:Glycine