Imbalance of CD45R and CDw29 helper cell subsets after seven days of culture from penicillin hypersensitive patients.
Specific PBMC proliferation to penicillin represents one of the in vitro diagnostic procedures to show antigenic sensitization. Nine patients with suggestive clinical history of penicillin hypersensitivity, significant stimulation index in the lymphocyte transformation test (LTT), and increased CD25 expression were selected. Their phenotypic expression of T-cell subset markers was compared with that of six normal subjects after 7 days of culture. Higher percentages of CD45R and CDw29 positive cells were found in penicillin-hypersensitive patients. This group of patients showed a significantly (P less than 0.01) higher percentage of CD45R positive cells compared with normal subjects, after 7 days of culture. The percentage of CD45R cells did not differ between patients and normal subjects at day 0, and the progressive decrease of CD45R positive cells in culture of normal subjects was not observable in our patients. These results suggest that the patients with penicillin hypersensitivity have an imbalance of CD45R/CDw29 ratio after 7 days of culture, with a higher expression of CD45R positive cells.
Perez
T
T
Dept. of Medicine, Hôpital Cantonal Universitaire, Geneva, Switzerland.
Girard
J P
JP
Dayer
E
E
D015703:Antigens, CD / Q000032:analysis*; D002455:Cell Division; D002478:Cells, Cultured; D004342:Drug Hypersensitivity / Q000209:etiology* / Q000276:immunology; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D019012:Integrin beta1; D017493:Leukocyte Common Antigens; D010400:Penicillin G / Q000009:adverse effects*; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D015703:Antigens, CD; D006649:Histocompatibility Antigens; D019012:Integrin beta1; D017493:Leukocyte Common Antigens; D010400:Penicillin G