Sodium and potassium ATPase of the teleost fish Catostomus commersoni. Sequence, protein structure and evolutionary conservation of the alpha-subunit.
The alpha-subunit of a Na+/K+ ATPase has been cloned by analysing a lambda gt11 library constructed from polyA+ RNA from the hypothalamic region of the teleost fish Catostomus commersoni (white sucker). The cDNA clone consists of 3853 bp and predicts a protein of 1027 amino-acid residues. Alignment of the sucker sequence with protein sequences previously published for alpha-subunits from various species reveals a high degree of homology throughout the entire sequence containing five potential sites for N-glycosylation, a phosphorylation site and a site for binding fluorescein 5'-isothiocyanate (FITC). A hydropathy profile predicts a secondary structure of the Na+/K+ ATPase alpha-subunit with at least eight membrane-spanning domains. Northern and southern blot analyses suggest the existence of two distinct Na+/K+ ATPase alpha-subunit genes in the sucker genome.
Schönrock
C
C
Institut für Zeilbiochemie und Klinische Neurobiologie, UKF, Universitat Hamburg.
Morley
S D
SD
Okawara
Y
Y
Lederis
K
K
Richter
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005399:Fishes / Q000235:genetics / Q000378:metabolism*; D007031:Hypothalamus / Q000201:enzymology*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012313:RNA / Q000235:genetics; D016415:Sequence Alignment; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry / Q000235:genetics*
D012313:RNA; D004247:DNA; D000254:Sodium-Potassium-Exchanging ATPase