Determination of chlorhexidine in saliva and in aqueous solutions.
A new method is presented for the determination of chlorhexidine in centrifuged saliva and in aqueous solutions by means of fluorescence spectroscopy. The method relies on complex formation between chlorhexidine and eosin. The fluorescence value of the chlorhexidine-eosin system decreases with increasing chlorhexidine concentrations. In centrifuged saliva a linear relation was found between the fluorescence at 541 nm and the chlorhexidine content up to about 15 ppm; the reproducibility of the method was found to be better than 1 ppm chlorhexidine. The biological spreading for centrifuged saliva from different participants as well as the spreading due to the period of saliva collection are about 3%. In whole uncentrifuged saliva the fluorescence method has been a detection limit of about 8 ppm chlorhexidine, due to the binding of the compound to salivary constituents. Above 8 ppm chlorhexidine, the biological variation in saliva is such that chlorhexidine determinations by means of fluorescence can be done only with a reproducibility of +/- 10 ppm. Advantages and disadvantages of the ultraviolet spectroscopy method, the radioactive labelling technique, and the fluorescence method for chlorhexidine determinations are discussed.
de Vries
J
J
Laboratory for Materia Technica, University of Groningen, The Netherlands.
Ruben
J
J
Arends
J
J
D002498:Centrifugation; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000032:analysis; D004801:Eosine Yellowish-(YS); D006801:Humans; D012463:Saliva / Q000737:chemistry*; D012996:Solutions / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D012996:Solutions; C010882:chlorhexidine gluconate; D002710:Chlorhexidine; D004801:Eosine Yellowish-(YS)