Characterization by somatic cell genetics of a monoclonal antibody to the MDR1 gene product (P-glycoprotein): determination of P-glycoprotein expression in multi-drug-resistant KB and CEM cell variants.
We isolated an IgG2a murine monoclonal antibody (MAb) termed MAb57, specifically reactive with multi-drug-resistant (MDR) human cells. Its specificity toward the MDRI gene product (P-glycoprotein) has been demonstrated by the concordant segregation of the MAb57 epitope with the MDRI gene in interspecific mouse x human cell hybrids, and the reactivity of several different MDRI gene-expressing cells with MAb57, particularly insect cells acutely infected with a baculovirus encoding the MDRI gene. MAb57 can be used to detect, by flow cytometry, variations in the relative drug-resistance levels of several MDR KB and CEM cell variants. This immunological probe has also proven useful in selectively destroying MDR target cells in an antibody-dependent cell-mediated (ADCC) assay system as well as in detecting P-glycoprotein expression in normal and malignant tissues and cells.
Cenciarelli
C
C
Laboratorio di Immunologia, Istituto Superiore di Sanità, Rome, Italy.
Currier
S J
SJ
Willingham
M C
MC
Thiebaut
F
F
Germann
U A
UA
Rutherford
A V
AV
Gottesman
M M
MM
Barca
S
S
Tombesi
M
M
Morrone
S
S
Santoni
A
A
Mariani
M
M
Ramoni
C
C
Dupuis
M L
ML
Cianfriglia
M
M
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002460:Cell Line; D004351:Drug Resistance; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007624:KB Cells; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D000939:Epitopes; D008562:Membrane Glycoproteins