title: Expression of plasmolipin in oligodendrocytes.
abstract: Plasmolipin is a plasma membrane proteolipid which has recently been described as a component of myelin (Cochary et al.: Journal of Neurochemistry 55:602-610, 1990). The present study reports the expression and localization of plasmolipin in primary glial cultures and secondary oligodendrocyte cultures. Double-label immunofluorescence showed that plasmolipin was expressed by galactocerebroside (GC)-positive oligodendrocytes, but was absent from astrocytes, characterized by their positive staining for glial fibrillary acidic protein (GFAP). At 1 week in culture plasmolipin staining was relatively weak in the cell body of some of the GC-positive cells. During the following 3 weeks in culture plasmolipin staining of oligodendrocytes gradually increased and was present in the cell body, its plasma membrane, and all the processes. However, the plasmolipin antibodies did not stain regions of the flat membrane sheets. Western blot analysis of homogenates from primary glial cultures showed that plasmolipin levels gradually increased during the first 5 weeks in culture. We conclude that the presence of plasmolipin in myelin is a result of its expression by oligodendrocytes.
authors.affiliation: Department of Biochemistry, Eunice Kennedy Shriver Center, Waltham, MA 02254.
authors.forename: I
authors.initials: I
authors.lastname: Fischer
authors.forename: E F
authors.initials: EF
authors.lastname: Cochary
authors.forename: J T
authors.initials: JT
authors.lastname: Konola
authors.forename: G
authors.initials: G
authors.lastname: Romano-Clark
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000737:chemistry; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005699:Galactosylceramides / Q000032:analysis; D005786:Gene Expression Regulation; D008565:Membrane Proteins; D051379:Mice; D004676:Myelin Basic Protein / Q000096:biosynthesis; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism*; D011510:Proteolipids / Q000096:biosynthesis*; D051381:Rats
chemical_list: D005699:Galactosylceramides; D008565:Membrane Proteins; D004676:Myelin Basic Protein; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D009419:Nerve Tissue Proteins; C489075:Pllp protein, mouse; C489076:Pllp protein, rat; D011510:Proteolipids