Antibody to histo-blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood group B or O donors.
Three virus isolates HTLV-IIIB/lyA, HTLV-IIIB/lyB and HTLV-IIIB/lyO, obtained by passaging and propagating the HTLV-IIIB/H9 isolate in three separate cultures of mixed peripheral blood mononuclear cells (PBMC) from donors of blood type A, B or O, respectively, were tested for susceptibility for virus neutralization by the monoclonal antibody (MAb) AH16 directed against the blood group A epitope. MAb AH16 was previously shown to inhibit cell-free virus infection using HTLV-IIIB propagated in H9 cells. AH16 showed a concentration-dependent inhibition of the HTLV-IIIB/lyA isolate but did not inhibit the HTLV-IIIB/lyB or the HTLV-IIIB/lyO isolate. Specificity of the MAb-mediated inhibition was shown using A-antigen (tetrasaccharide). Thus, HIV infection of PBMC from donors with blood type A appears to induce expression of host-cell-encoded carbohydrate blood group A epitope on HIV which can be a target for MAb-mediated virus neutralization.
Arendrup
M
M
Department of Infectious Diseases 144, Hvidovre Hospital, University of Copenhagen, Denmark.
Hansen
J E
JE
Clausen
H
H
Nielsen
C
C
Mathiesen
L R
LR
Nielsen
J O
JO
D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001782:Blood Donors; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D000939:Epitopes; D006031:Glycosylation; D006678:HIV / Q000254:growth & development / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D009500:Neutralization Tests
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D015488:HIV Antigens