The characterization of synthetic by-products by B/E linked scanning and high-resolution thermospray mass spectrometry.
Characterization of unknown synthetic by-products from a test synthesis of the experimental inhibitor of HIV-1 reverse transcriptase, BI-RG-587, is presented. Seven impurities were separated and characterized by thermospray liquid chromatography/mass spectrometry using low-resolution, high-resolution, and B/E linked scanning methods. The high-resolution mass measurements which were obtained were accurate to within 10 ppm of structures proposed for synthetic by-products present at the 0.1% level. Linked scans produced daughter ion spectra for each impurity to further aid in the characterization.
Dinallo
R M
RM
Department of Analytical Sciences, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, Connecticut 06877.
Davidson
W C
WC
Hansen
G E
GE
D000998:Antiviral Agents / Q000138:chemical synthesis*; D015497:HIV-1 / Q000201:enzymology*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D019829:Nevirapine; D011725:Pyridines / Q000138:chemical synthesis*; D018894:Reverse Transcriptase Inhibitors
D000998:Antiviral Agents; D011725:Pyridines; D018894:Reverse Transcriptase Inhibitors; D019829:Nevirapine