Goat antibodies to amino acid sequences of human chorionic gonadotropin (hCG)
Human chorionic gonadotropin, its two subunits and its conjugate with thyroglobulin were used for immunization. The strongest immunogens were demonstrated to be the intact hormone and beta subunit, the weakest was alpha subunit. Using three peptides corresponding to hormone subunits coupled to Sepharose 4B various antibodies were isolated from goat antiserum by immunoaffinity chromatography. Specificity of the purified antibody preparations was studied. It was demonstrated that enzyme linked immunosorbent assay with two goat antibodies--one for coating of microtiter plates and the other one conjugated with peroxidase--is suitable for sensitive assays of both human chorionic gonadotropin and luteinizing hormone. Specific and sensitive assays were performed using combination of goat antibody anti-122-145-beta hCG and monoclonal antibody anti-alpha hCG.
Kochanowska
I E
IE
Biochemical Laboratory, Institute of Immunology and Experimental Therapy, Wrocław, Poland.
Rapak
A
A
Maćkiewicz
Z
Z
Kupryszewski
G
G
Szewczuk
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000941:Antigens; D006063:Chorionic Gonadotropin / Q000032:analysis / Q000737:chemistry* / Q000276:immunology*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D015292:Glycoprotein Hormones, alpha Subunit / Q000737:chemistry / Q000276:immunology; D006041:Goats; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D015292:Glycoprotein Hormones, alpha Subunit; D010446:Peptide Fragments