Studies on glycoprotein 13 (gp13) of equid herpesvirus 1 using affinity-purified gp13, glycoprotein-specific monoclonal antibodies and synthetic peptides in a hamster model.
Hamsters were immunized with either an affinity-purified preparation of equid herpesvirus 1 (EHV-1) glycoprotein 13 (gp13) or synthetic peptides representing three sequences within the homologous glycoprotein of EHV-4, resulting in the production of anti-peptide (in the case of peptide-immunized animals) or antivirus antibodies. The sera from gp13-immunized hamsters contained antibodies which showed virus-neutralizing activity and complement-mediated antibody lysis of EHV-1-infected target cells. These hamsters were protected from EHV-1 challenge. The characteristics of a panel of anti-gp13 monoclonal antibodies (P28, P17, 14H7, 16E4 and 16H9) were assessed both in vivo and in vitro. 16E4 and P28 showed high levels of complement-mediated neutralization of virus, complement-mediated lysis of virus-infected target cells and passive protection of hamsters. Furthermore, epitope mapping studies demonstrated that this glycoprotein contains a neutralizing epitope recognized by EHV-1-immune horse serum. The data imply that gp13 has potential as a candidate antigen for a molecular vaccine.
Stokes
A
A
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, U.K.
Corteyn
A H
AH
Pullen
L A
LA
Doel
T R
TR
Meredith
D M
DM
Killington
R A
RA
Halliburton
I W
IW
Whittaker
G R
GR
Wheldon
L A
LA
Nicolson
L
L
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D002846:Chromatography, Affinity; D003165:Complement System Proteins; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D004861:Herpesvirus 1, Equid / Q000276:immunology* / Q000302:isolation & purification; D007116:Immunization, Passive; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008647:Mesocricetus; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010455:Peptides / Q000138:chemical synthesis; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010455:Peptides; D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; D003165:Complement System Proteins