Identification of coagulase-negative staphylococci by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and rRNA restriction patterns.
A total of 1,417 staphylococcal and micrococcal strains were collected from the beards and scalps of 10 subjects over a period of 8 months. Sixteen strains identified as Staphylococcus epidermidis with an API system had distinctive yellow colonies on nutrient agar plates and sodium dodecyl sulfate-polyacrylamide gel electrophoresis whole-cell polypeptide profiles similar to those of Staphylococcus capitis; this identification was confirmed by analysis of rRNA gene restriction patterns.
Pennington
T H
TH
Department of Medical Microbiology, University of Aberdeen, Scotland.
Harker
C
C
Thomson-Carter
F
F
D001426:Bacterial Proteins / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D003030:Coagulase / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D013210:Staphylococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D001426:Bacterial Proteins; D003030:Coagulase; D012329:RNA, Bacterial; D012335:RNA, Ribosomal