Enhancement by IL-1 beta and IFN-gamma of platelet activation: adhesion to leukocytes via GMP-140/PADGEM protein (CD62).
We have examined the effect of inflammatory cytokines on the platelet activation. IL-1 beta and IFN-gamma were found to enhance the adhesion of thrombin-treated platelets to monocytic leukemia cells (U937), when the adhesion was assayed by platelet-mediated cell agglutination. The agglutination was inhibited by a monoclonal anti-GMP140 antibody or EDTA, suggesting that the enhanced platelet adhesion to the leukemic cells was mediated by GMP140. In addition, these cytokines also increased the release of 5-HT from platelets in the presence of a low concentration of thrombin. These data suggest that platelet functions are regulated by the cytokines and that activated platelets participate in inflammatory process.
Todoroki
N
N
Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Watanabe
Y
Y
Akaike
T
T
Katagiri
Y
Y
Tanoue
K
K
Yamazaki
H
H
Tsuji
T
T
Toyoshima
S
S
Osawa
T
T
D001792:Blood Platelets / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D009000:Monocytes / Q000502:physiology*; D019007:P-Selectin; D015539:Platelet Activation / Q000187:drug effects*; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology
D015815:Cell Adhesion Molecules; D007375:Interleukin-1; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D011994:Recombinant Proteins; D007371:Interferon-gamma