The 5'-untranslated region of p23 mRNA from the Ehrlich ascites tumor is involved in translation control of the growth related protein p23.
The growth-related protein p23 of the Ehrlich ascites tumor (EAT) is preferentially expressed in the exponentially growing tumor; its synthesis is translationally controlled. p23 mRNA is efficiently translated in the wheat germ cell-free lysate. In contrast, p23 mRNA present in poly(A)+RNA isolated from EAT is not translated in cell-free systems of EAT and reticulocytes. Moreover, translation of a p23 transcript is inhibited in the presence of total poly(A)+RNA. This inhibition is abolished by the removal of the 5'-UTR of the p23 transcript. Solution hybridization/RNase protection experiments point to the presence of a nucleotide sequence complementary to the 5'-UTR of p23 mRNA which might be involved in p23 mRNA inhibition.
Böhm
H
H
Central Institute of Molecular Biology, Department of Cell Physiology, Berlin-Buch.
Gross
B
B
Gaestel
M
M
Bommer
U A
UA
Ryffel
G
G
Bielka
H
H
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics* / Q000473:pathology; D002455:Cell Division; D002474:Cell-Free System; D002872:Chromosome Deletion; D051379:Mice; D009865:Oocytes / Q000378:metabolism; D010957:Plasmids; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D015183:Restriction Mapping; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic; D014908:Triticum / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014981:Xenopus
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA