Synthesis of fusion proteins of Epstein-Barr virus nuclear antigens in E. coli and their antigenicity.
Expression and yield in E. coli of a panel of fusion proteins containing various domains of Epstein-Barr virus nuclear antigens, EBNA-1, EBNA-2, EBNA-3, EBNA-4 and EBNA-6, were scrutinized. The antigenicity of the EBNA fusion proteins against human sera was examined. Monospecific antisera to the different EBNA domains were produced by immunizing guinea pigs and rabbits. An EBNA-6 fusion polypeptide was useful for separating anti-EBNA-6 antibody from human sera by immunoaffinity purification. The applications of the fusion proteins to clinical diagnosis are discussed.
Inoue
N
N
Department of Virology and Rickettsiology, National Institute of Health, Tokyo, Japan.
Harada
S
S
Yanagi
K
K
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D000939:Epitopes; D019309:Epstein-Barr Virus Nuclear Antigens; D004926:Escherichia coli / Q000235:genetics; D006168:Guinea Pigs; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D001616:beta-Galactosidase / Q000235:genetics / Q000276:immunology
D000956:Antigens, Viral; D000939:Epitopes; D019309:Epstein-Barr Virus Nuclear Antigens; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase