Inhibitors of protein and RNA synthesis block the sensitization of murine peritoneal mast cells.
Addition of the protein synthesis inhibitor cycloheximide (CX, 1 microgram/ml) and the RNA synthesis inhibitor actinomycin D (AD, 0.1 microgram/ml) to unfractionated mouse peritoneal mast cells simultaneously with IgE anti-DNP, for 24 h prior to challenge, completely blocked antigen-induced 5-HT release. Responses to anti-IgE were strongly abrogated whereas responses to the calcium ionophore A23187 were not affected. When CX and AD were added to presensitized cells their effects on antigen and anti-IgE-induced release were much reduced. These results suggest a requirement for protein synthesis during mast cell sensitization.
Coleman
J W
JW
Department of Pharmacology and Therapeutics, University of Liverpool, UK.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D007073:Immunoglobulin E / Q000276:immunology*; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012701:Serotonin / Q000378:metabolism
D003609:Dactinomycin; D012701:Serotonin; D007073:Immunoglobulin E; D012313:RNA; D003513:Cycloheximide