title: Cowdria ruminantium is recognized by a monoclonal antibody directed against the major outer membrane protein of Chlamydia trachomatis.
abstract: The relationship between Cowdria ruminantium and Chlamydia trachomatis was studied by immunofluorescence. A monoclonal antibody directed against the major outer membrane protein of C. trachomatis recognized rickettsial colonies of C. ruminantium in infected goat brain. No specific fluorescence was observed in non-infected brain. Two commercial Chlamydia-specific monoclonal antibodies as well as polyvalent anti-Chlamydia rabbit serum recognized C. trachomatis, but did not recognize Cowdria. Moreover, polyvalent Cowdria antiserum failed to recognize C. trachomatis cultivated in HeLa cells. It is concluded that Cowdria and Chlamydia are to a certain extent related, confirming similarities in ultrastructure and developmental cycle.
authors.affiliation: Department of Tropical Veterinary Medicine and Protozoology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht, Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: Jongejan
authors.forename: R
authors.initials: R
authors.lastname: Bax
authors.forename: M J
authors.initials: MJ
authors.lastname: Meddens
authors.forename: W G
authors.initials: WG
authors.lastname: Quint
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001921:Brain / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D015511:Goat Diseases / Q000276:immunology / Q000382:microbiology; D006041:Goats; D006367:HeLa Cells; D006357:Heartwater Disease / Q000276:immunology / Q000382:microbiology; D007106:Immune Sera / Q000276:immunology; D010802:Phylogeny; D012287:Rickettsiaceae / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D007106:Immune Sera