Two regions of M13 phage genome hybridizing with human DNA are similar to several keratin genes.
DNA from two regions of the phage M13 genome hybridizes with DNA restriction fragments from genomes of various species including man [15, 20]. As the pattern of hybridization is individual-specific, this phage M13 probe can be used for DNA fingerprinting. We demonstrate here that the regions of many keratin genes coding for glycine-rich parts of C and N end domains are very similar to the phage M13 probe, and this similarity may be responsible for hybridization.
Kondrashov
A S
AS
Research Computer Center Academy of Sciences USSR, Moscow Region.
Beridze
T G
TG
Chiaureli
N B
NB
D000818:Animals; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D007633:Keratins / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D015342:DNA Probes; D004279:DNA, Viral; D007633:Keratins; D004247:DNA