RecBC promoted repair of bleomycin damage in Escherichia coli.
The repair response of Escherichia coli K-12 to bleomycin was examined in Rec- mutants showing differential sensitivity to this agent. Sedimentation analysis of the cellular DNA showed incision after bleomycin treatment. The subsequent reformation of the DNA, found in the wild-type and the recD mutant, was abolished in the recB and delayed in the recF and recBC sbcB mutants. The bleomycin-induced SOS response was reduced in strains containing recB or recBC sbsB mutations. It is suggested that the RecBCD pathway has the main role in the efficient repair of bleomycin-induced DNA damage.
Knezević-Vukcević
J
J
Laboratory for Microbiology, Faculty of Science, University of Belgrade, Yugoslavia.
Simić
D
D
D001761:Bleomycin / Q000494:pharmacology*; D004249:DNA Damage; D004265:DNA Helicases / Q000187:drug effects / Q000235:genetics*; D004260:DNA Repair; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial; D011995:Recombination, Genetic
D029968:Escherichia coli Proteins; D001761:Bleomycin; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli; D004265:DNA Helicases