[A highly sensitive immunoenzyme analysis using electrochemical detection].
The authors have developed a method for improving the sensitivity of electrochemical enzyme immunoassay, based on binding the enzymatic reaction volatile component to a complexone, followed by destruction of the complex in a flow-type amperometric detector. The lowest limit of the measured levels of blood serum antibodies and antigens is 10(-11)-10(-12) M. The method was tried in enzyme immunoassay of the blood serum IgE in patients with asthma and chronic obstructive bronchitis. Reduction of IgE level after therapy was found to be a regular feature; the authors claim that it may be considered as one of the criteria indicating the treatment efficacy.
Sitdykov
R A
RA
Ivnitskiĭ
D M
DM
Davidian
A A
AA
Valieva
R U
RU
D000328:Adult; D001249:Asthma / Q000097:blood; D001991:Bronchitis / Q000097:blood; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000097:blood; D008875:Middle Aged
D007073:Immunoglobulin E
Vysokochuvstvitel'nyĭ immunofermentnyĭ analiz s élektrokhimicheskim detektirovaniem.