Glycosylation and transmembrane topography of bovine chromaffin granule p65.
The bovine homologue of p65, a calmodulin-binding protein located in the membranes of synaptic vesicles and endocrine secretory granules, has been studied by the use of monoclonal antibodies directed against this antigen and against dopamine beta-mono-oxygenase. The protein (apparent molecular mass 67 kDa; pI = 5.5-6.2) is partially degraded by treatment with neuraminidase or endoglycosidase F. Trypsin treatment of intact adrenal chromaffin granules or of granule membranes releases a soluble 39 kDa fragment of p65 which corresponds to the whole of its cytoplasmic domain. This domain contains both the epitope for the monoclonal antibody cgm67 and the calmodulin-binding site. The 20 amino acids at the N-terminus of this fragment are identical to part of the rat p65 sequence.
Tugal
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HB
Department of Biochemistry, University of Edinburgh Medical School, Scotland, U.K.
van Leeuwen
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Apps
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Haywood
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J
Phillips
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JH
D000595:Amino Acid Sequence; D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000737:chemistry* / Q000276:immunology; D002417:Cattle; D002837:Chromaffin Granules / Q000737:chemistry* / Q000378:metabolism; D003598:Cytoskeletal Proteins; D003600:Cytosol / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006031:Glycosylation; D007425:Intracellular Membranes / Q000737:chemistry* / Q000378:metabolism; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011487:Protein Conformation
D002148:Calmodulin-Binding Proteins; D003598:Cytoskeletal Proteins; D000939:Epitopes; D008840:Microfilament Proteins; D010750:Phosphoproteins; C066559:Lcp1 protein, mouse