Hepatocyte growth factor is a potent mitogen for cultured rabbit renal tubular epithelial cells.
Hepatocyte growth factor (HGF), which is a potent growth factor of adult rat hepatocytes in primary culture, also strongly stimulated DNA synthesis of rabbit renal tubular epithelial cells in secondary culture. Its mitogenic activity was dose-dependent, being detectable at 3 ng/ml and maximal at 30 ng/ml. Over 20% of the cells were shifted to the S-phase by HGF alone, judging by the labeling index. HGF had additive effects with EGF, acidic fibroblast growth factor (a-FGF), and insulin. Transforming growth factor-beta 1 (TGF-beta 1) strongly inhibited DNA synthesis of renal tubular cells stimulated by HGF. The growth of renal tubular epithelial cells was also regulated by cell density: DNA synthesis stimulated by HGF was high at lower cell density and was strongly suppressed at high cell density. These results suggest that HGF may act as a renotropic factor in compensatory renal growth or renal regeneration in vivo.
Igawa
T
T
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Kanda
S
S
Kanetake
H
H
Saitoh
Y
Y
Ichihara
A
A
Tomita
Y
Y
Nakamura
T
T
D000818:Animals; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D017228:Hepatocyte Growth Factor; D007065:Idoxuridine / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D007672:Kidney Cortex / Q000166:cytology* / Q000187:drug effects; D007684:Kidney Tubules / Q000166:cytology* / Q000187:drug effects; D007700:Kinetics; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D006133:Growth Substances; D007328:Insulin; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D016220:Fibroblast Growth Factor 1; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D007065:Idoxuridine