Charting of type II glucocorticoid receptor-like immunoreactivity in the rat central nervous system.
The rat brain and spinal cord have been mapped for Type II glucocorticoid receptor-like immunoreactivity in neurons and glia, using a monoclonal antibody, BUGR2, which recognizes an epitope close to the DNA-binding domain of the rat Type II receptor. The study revealed a widespread distribution of Type II-like immunoreactive neurons and glia, and a heterogeneity of densities and intensities of immunoreactive elements. Our results corresponded to a large extent with previous immunocytochemical mapping using Ig2a, a monoclonal antibody against a different epitope in the variable domain, with some notable differences in the hippocampus, hypothalamus and cerebellum. There was also a good correlation between immunocytochemical mapping and binding studies, [3H]steroid autoradiography and mRNA localization of the Type II receptor.
Ahima
R S
RS
Department of Anatomy, Tulane University School of Medicine, New Orleans, LA 70112.
Harlan
R E
RE
D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology; D000939:Epitopes / Q000032:analysis; D008297:Male; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D011965:Receptors, Glucocorticoid / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D013116:Spinal Cord / Q000033:anatomy & histology* / Q000166:cytology; D014316:Tritium
D000911:Antibodies, Monoclonal; D000939:Epitopes; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D014316:Tritium