[Single cell fluorescence photometry for quantification of antigens on alveolar macrophages].
The quantitation of cell surface antigens on alveolar macrophages (AM) using flow-cytometry is complicated by strong autofluorescence and wide variation of cell- size and autofluorescence. Therefore a microscope-fluorometric method was developed which allows simultaneous assessment of fluorescence of fluorochrome-labelled monoclonal antibodies and size in individual cells. Autofluorescence of smokers AM was found to be variable and considerably stronger than autofluorescence of nonsmokers AM. By means of a multi step determination of autofluorescence and specific fluorescence using different filter combinations of the microscope fluorometer density of membrane bound HLADR-antigens could be efficiently determined also in strongly autofluorescent smoker AM.
Pankow
W
W
Zentrum für Innere Medizin, Philipps-Universität Marburg.
Neumann
K
K
Rüschoff
J
J
von Wichert
P
P
D000954:Antigens, Surface / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000473:pathology; D011650:Pulmonary Alveoli / Q000276:immunology / Q000473:pathology*; D012016:Reference Values; D012907:Smoking / Q000276:immunology / Q000473:pathology*; D007507:Therapeutic Irrigation
D000954:Antigens, Surface
Einzelzell-Fluoreszenzphotometrie zur Antigenquantifizierung auf Alveolarmakrophagen.