title: Na current density at and away from end plates on rat fast- and slow-twitch skeletal muscle fibers.
abstract: Na current density and membrane capacitance were studied with the loose patch voltage clamp technique on rat fast- and slow-twitch skeletal muscle fibers at three different regions on the fibers: 1) the end plate border, 2) greater than 200 microns from the end plate (extrajunctional), and 3) on the end plate postsynaptic membrane. Fibers were treated with collagenase to improve visualization of the end plate and to enzymatically remove the nerve terminal. The capacitance of membrane patches was similar on fast- and slow-twitch fibers and patches of membrane on the end plate had twice the capacitance of patches elsewhere. For fast- and slow-twitch fibers, the sizes of the Na current normalized to the area of the patch were as follows: end plate greater than end plate border greater than extrajunctional. For both types of fibers, the amplitudes of the Na current normalized to the capacitance of the membrane patch were as follows: end plate approximately end plate border greater than extrajunctional. At each of the three regions, the Na current densities were larger on fast-twitch fibers and fast-twitch fibers had a larger increase in Na current density at the end plate border compared with extrajunctional membrane.
authors.affiliation: Department of Neurology, Cleveland Veterans Affairs Medical Center, Ohio.
authors.forename: R L
authors.initials: RL
authors.lastname: Ruff
mesh_terms: D000818:Animals; D004553:Electric Conductivity; D004594:Electrophysiology; D008297:Male; D009045:Motor Endplate / Q000502:physiology*; D009119:Muscle Contraction; D009132:Muscles / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000502:physiology*; D013997:Time Factors
chemical_list: D012964:Sodium