Immunoblot analysis demonstrates that the large and small forms of hepatitis delta virus antigen have different C-terminal amino acid sequences.
Antisera to a peptide representing the extreme carboxy terminus of the hepatitis delta virus antigen (HDAg) open reading frame (residues 197 to 211) recognized only the large (p27 delta) and not the small (p24 delta) form of HDAg in immunoblots of infected liver extracts, thereby providing direct proof that p27 delta and p24 delta differ in their carboxyl-terminal sequence and that p27 delta results from mutation within the stop codon terminating translation of p24 delta. Reactions with other peptide antisera demonstrated that multiple smaller virus-specified proteins were carboxy-terminally truncated forms of HDAg, and immunoprecipitation studies suggested that different forms of HDAg were present as heterologous complexes within the liver extract.
Wang
J G
JG
Department of Medicine, School of Medicine, University of North Carolina, Chapel Hill 27599-7030.
Cullen
J
J
Lemon
S M
SM
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000097:blood / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D003062:Codon / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D003699:Hepatitis D / Q000276:immunology / Q000382:microbiology*; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000276:immunology*; D035923:Hepatitis delta Antigens; D015151:Immunoblotting; D008099:Liver / Q000276:immunology / Q000382:microbiology; D008392:Marmota; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011233:Precipitin Tests
D000956:Antigens, Viral; D003062:Codon; D035923:Hepatitis delta Antigens; C442244:hepatitis delta virus large antigen