Characterization of a U-937 subline which can be induced to differentiate in serum-free medium.
We report the selection and characterization of a U-937 subline which is capable of long-term growth in serum-free medium and can be induced to differentiate. The subline (U-937-1SF) can be maintained in standard RPMI-1640 medium supplemented by antibiotics only. As compared to the serum-dependent U-937 parental cell line, U-937-1SF produced lower amounts of lysozyme and elastase and had a decreased surface expression of complement receptor 1 (CD35) and myeloid antigens CDw17 and CD38. Apart from these alterations, the U-937-1SF cells appear to be morphologically, cytogenetically and phenotypically similar to the parental U-937 clone-1 cells. The capacity of U-937 clone-1 cells to undergo phorbol myristic acid (PMA)-, vitamin D3 (VitD3)- and retinoic-acid (RA)-induced differentiation was retained in the U-937-1SF cells as evidenced by the induced growth arrest, development of a monocyte/macrophage morphology and increased expression of differentiation-associated antigens, e.g. CD11b, CD11c, CD14 and CD18. The growth-inhibitory response to cytokines involved in the activation and differentiation of monocytes, IFN-gamma, TNF-alpha, IL-1 beta, IL-6 and GM-CSF, was normal. Our results suggest that the U-937-1SF subline can be used as a serum-free model system for studies on various aspects of monocyte differentiation.
Oberg
F
F
Department of Pathology, Uppsala University, University Hospital, Sweden.
Hult
N
N
Bjare
U
U
Ivhed
I
I
Kivi
S
S
Bergh
J
J
Larsson
L G
LG
Sundström
C
C
Nilsson
K
K
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D016895:Culture Media, Serum-Free; D016207:Cytokines / Q000235:genetics / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006410:Hematopoiesis; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D051379:Mice; D008819:Mice, Nude; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000473:pathology*; D014807:Vitamin D / Q000494:pharmacology
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D016895:Culture Media, Serum-Free; D016207:Cytokines; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014807:Vitamin D; D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate