Aggregation of human granulocytes by Staphylococcus aureus lipase.
The effects of purified extracellular lipase from Staphylococcus aureus on human granulocytes were studied in vitro with a turbidimetric technique. Within the concentration range 0.6-4.4 micrograms/ml, lipase caused monophasic aggregation accompanied by the release of lactoferrin; the corresponding concentrations of the solvent in which it was suspended, Triton X100, had no effect. Lipase-induced aggregation did not occur in the presence of autologous plasma.
Rollof
J
J
Department of Infectious Diseases, University Hospital of Lund, Sweden.
Vinge
E
E
Nilsson-Ehle
P
P
Braconier
J H
JH
D002449:Cell Aggregation / Q000502:physiology*; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D007781:Lactoferrin / Q000032:analysis; D008049:Lipase / Q000378:metabolism*; D017830:Octoxynol; D011092:Polyethylene Glycols; D013211:Staphylococcus aureus / Q000201:enzymology*; D013696:Temperature
D011092:Polyethylene Glycols; D017830:Octoxynol; D008049:Lipase; D007781:Lactoferrin