Generation of anti-peptide and anti-protein sera. Effect of peptide presentation on immunogenicity.
The technique of Fmoc chemistry has been applied successfully to the synthesis of branched peptides. The immunogenicity of branched peptides has been compared quantitatively with those of protein-conjugated and resin-linked peptides. Six different peptide sequences were used to immunise rabbits and both antipeptide and anti-protein titres were determined for each serum. The data show that the titres of sera from rabbits immunised with branched peptides were higher than those of rabbits immunised with protein-conjugated peptides which in turn were higher than those immunised with resin-linked peptides. The effect was demonstrated with two strains of rabbits.
McLean
G W
GW
Institute of Virology, Glasgow, Scotland, U.K.
Owsianka
A M
AM
Subak-Sharpe
J H
JH
Marsden
H S
HS
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000276:immunology; D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005449:Fluorenes; D007114:Immunization; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits; D018139:Simplexvirus / Q000276:immunology; D013329:Structure-Activity Relationship; D014764:Viral Proteins / Q000138:chemical synthesis / Q000276:immunology
D000596:Amino Acids; D000939:Epitopes; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010446:Peptide Fragments; D010455:Peptides; D014764:Viral Proteins