Solubilization of rat kidney lysosomes in reversed micelles of aerosol OT in octane.
Intact lysosomes from rat kidneys were solubilized in the ternary system: surfactant (Aerosol OT)-buffer-organic solvent. According to data of laser light-scattering analysis and kinetic experiments with the lysosomal marker enzyme, N-acetyl-beta-D-hexosaminidase (EC 3.2.1.30), the solubilization of lysosomes in this system resulted in the destruction of the lysosomes and the entrapping of their components in reversed micelles.
Pshezhetsky
A V
AV
Belozersky Laboratory, Moscow State University, USSR.
Buneeva
O A
OA
Wiederschain GYa
D000818:Animals; D004143:Dioctyl Sulfosuccinic Acid; D007668:Kidney / Q000648:ultrastructure*; D008247:Lysosomes / Q000201:enzymology*; D008823:Micelles; D009795:Octanes; D051381:Rats; D012995:Solubility; D013501:Surface-Active Agents; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D008823:Micelles; D009795:Octanes; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid; D001619:beta-N-Acetylhexosaminidases; C026728:octane