title: Detection of infectious agents by molecular methods at the cellular level.
abstract: The role of molecular methods for the detection of infectious agents at the cellular level continues to grow. For the future PCR and in situ PCR methods appear to hold great potential as adjuncts to current methods, but not without overcoming some obstacles.
authors.affiliation: Department of Pathology, New England Medical Center Hospitals, Tufts University, Boston, MA 02111.
authors.forename: H J
authors.initials: HJ
authors.lastname: Wolfe
authors.forename: D
authors.initials: D
authors.lastname: Ross
authors.forename: B
authors.initials: B
authors.lastname: Wolfe
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000473:pathology*; D004279:DNA, Viral / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis / Q000473:pathology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004279:DNA, Viral