title: Coexistence of autologous antibodies and decay-accelerating factor, an inhibitor of complement, on human renal tumor cells.
abstract: Autologous immunoglobulin was detected on the cell surface of tumor cells freshly isolated from cancerous kidneys of patients with renal cell carcinoma by flow cytometry after staining with murine anti-human IgG monoclonal antibodies. Cells isolated in parallel from macroscopically normal regions of the tumorous kidneys were not specifically stained with the anti-human IgG reagents. In further studies, tumor cells were stained with an antibody to decay-accelerating factor (DAF), a known inhibitor of complement. Flow cytometry of these cells revealed that nearly all tumor cells expressed DAF, and that the intensity of staining with the anti-DAF monoclonal antibody correlated with the staining of cells with anti-IgG. The results suggest that tumor cells coated with autologous antibody may be resistant to complement-mediated cytotoxicity in vivo through the expression of high levels of DAF.
authors.affiliation: Section of Immunology, Cleveland Clinic Foundation, Ohio 44195.
authors.forename: T
authors.initials: T
authors.lastname: Terachi
authors.forename: G
authors.initials: G
authors.lastname: Stanescu
authors.forename: J E
authors.initials: JE
authors.lastname: Pontes
authors.forename: M E
authors.initials: ME
authors.lastname: Medof
authors.forename: M J
authors.initials: MJ
authors.lastname: Caulfield
mesh_terms: D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000032:analysis*; D018958:CD55 Antigens; D003169:Complement Inactivator Proteins / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D007680:Kidney Neoplasms / Q000276:immunology* / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology; D008565:Membrane Proteins / Q000032:analysis*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D018958:CD55 Antigens; D003169:Complement Inactivator Proteins; D008565:Membrane Proteins; D011947:Receptors, Antigen, B-Cell