title: Protection from chemical modification of nucleotides in complexes of M1 RNA, the catalytic subunit of RNase P from E coli, and tRNA precursors.
abstract: Certain nucleotides in M1 RNA, the catalytic RNA subunit of RNase P from E coli, are protected from chemical modification when M1 RNA forms complexes with tRNA precursor molecules (ES complexes). Many of these nucleotides are important in the formation of the Michaelis complex. In the presence of tRNA precursor molecules, the pattern of protection from chemical modification of a region in M1 RNA that resembles the E site in 23S rRNA is similar to the pattern of protection of the E site in the presence of deacylated tRNA. In the complex with the RNA enzyme, more nucleotides in the substrate become accessible to modification, an indication that the substrate is in an unfolded conformation under these conditions.
authors.affiliation: Department of Biology, Yale University, New Haven, CT 06520.
authors.forename: A K
authors.initials: AK
authors.lastname: Knap
authors.forename: D
authors.initials: D
authors.lastname: Wesolowski
authors.forename: S
authors.initials: S
authors.lastname: Altman
mesh_terms: D001483:Base Sequence; D004722:Endoribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D008961:Models, Structural; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000378:metabolism*; D012329:RNA, Bacterial / Q000378:metabolism*; D016337:RNA, Catalytic / Q000378:metabolism*; D012338:RNA, Ribosomal, 23S / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism*; D043262:Ribonuclease P; D013463:Sulfuric Acid Esters / Q000494:pharmacology
chemical_list: D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D012322:RNA Precursors; D012329:RNA, Bacterial; D016337:RNA, Catalytic; D012338:RNA, Ribosomal, 23S; D013463:Sulfuric Acid Esters; D012343:RNA, Transfer; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli; C007482:dimethyl sulfate