Retarded Wallerian degeneration following peripheral nerve transection in C57BL/6/Ola mice is associated with delayed down-regulation of the P0 gene.
Wallerian degeneration is markedly retarded in C57BL/6/Ola mice, the majority of axons remaining intact for up to 10 days after sciatic transections. By 5 days after axotomy in normal mice P0 mRNA is markedly down-regulated, whereas high expression is present in the mutant mice at 7 days and is not reduced by a second distal axotomy. However, dissociated Schwann cells cultured without neurons down-regulate P0 in the normal manner. The data suggest that the continued expression of P0 mRNA is probably dependent on a relatively stable axonal signal with a low turnover that does not require continuous fast anterograde or retrograde transport. This may indicate an intrinsic axolemmal molecule(s) is involved in the neuronal signal.
Thomson
C E
CE
Applied Neurobiology Group, University of Glasgow, Bearsden, Scotland, U.K.
Mitchell
L S
LS
Griffiths
I R
IR
Morrison
S
S
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D003714:Denervation; D005786:Gene Expression Regulation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants; D018993:Myelin P0 Protein; D009185:Myelin Proteins / Q000235:genetics*; D009474:Neurons / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012016:Reference Values; D012584:Sciatic Nerve / Q000502:physiology*; D014855:Wallerian Degeneration
D018993:Myelin P0 Protein; D009185:Myelin Proteins; D012333:RNA, Messenger