Complete cDNA and gene sequence of the developmentally regulated arylphorin of Calliphora vicina and its homology to insect hemolymph proteins and arthropod hemocyanins.
Two cDNA libraries were prepared from poly(A)+ RNA isolated from fat bodies of last instar larvae of the blowfly Calliphora vicina. The libraries were probed with a genomic clone containing the coding sequence for an arylphorin subunit. Two cDNA clones as well as the genomic clone were mapped and their nucleotide sequences were determined. This revealed the presence of an open reading frame corresponding to a polypeptide with 759 amino acid residues. The deduced primary structure of Calliphora arylphorin and hemolymph proteins of other insect species and arthropod hemocyanine show nearly 30% identity. Highly conserved regions could be also identified.
Naumann
U
U
Institute of Zoology, Developmental Biology, University of Würzburg, Germany.
Scheller
K
K
D000273:Adipose Tissue / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001181:Arthropods / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004175:Diptera / Q000235:genetics* / Q000254:growth & development; D005786:Gene Expression Regulation; D015723:Gene Library; D005796:Genes; D006023:Glycoproteins / Q000235:genetics; D006433:Hemocyanins / Q000235:genetics*; D006458:Hemolymph / Q000502:physiology; D007301:Insect Hormones / Q000235:genetics*; D019476:Insect Proteins; D007313:Insecta / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D011506:Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D006023:Glycoproteins; D007301:Insect Hormones; D019476:Insect Proteins; D046911:Macromolecular Substances; D011506:Proteins; D012333:RNA, Messenger; C041137:storage proteins, Insecta; D011061:Poly A; D012313:RNA; D004247:DNA; D006433:Hemocyanins