A comparison of WIN 51711 and R 78206 as stabilizers of poliovirus virions and procapsids.
The thermal denaturation of poliovirus virions and procapsids in the 42 to 48 degrees C range was studied using N- and H-specific monoclonal antibodies. The half-life of the N antigen of Mahoney and Sabin 1 virions was extended 50- to 250-fold by either 10 microM WIN 51711 or R 78206. The minimum concentrations required for full stabilization at 46 degrees C (1.0 microM for WIN 51711, 0.2 microM for R 78206) were independent of the strain or serotype of the virus; 30 to 60 molecules of stabilizer per virion were required for full protection. R 78206 was the most efficient stabilizer of Mahoney procapsids; the half-life of the N-specific epitopes of these particles at 44 degrees C was extended from less than 1 min to 1 day.
Rombaut
B
B
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
Andries
K
K
Boeyé
A
A
D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002213:Capsid / Q000187:drug effects / Q000378:metabolism; D000939:Epitopes / Q000378:metabolism; D006207:Half-Life; D007555:Isoxazoles / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D010880:Piperidines / Q000737:chemistry / Q000494:pharmacology*; D017955:Poliovirus / Q000187:drug effects*; D011498:Protein Precursors / Q000187:drug effects; D011724:Pyridazines / Q000737:chemistry / Q000494:pharmacology*; D014758:Viral Core Proteins / Q000378:metabolism; D014771:Virion / Q000187:drug effects*
D000998:Antiviral Agents; D000939:Epitopes; D007555:Isoxazoles; D010880:Piperidines; D011498:Protein Precursors; D011724:Pyridazines; D014758:Viral Core Proteins; C070921:R 78206; C045978:disoxaril