title: Interaction between 16S ribosomal RNA and ribosomal protein S12: differential effects of paromomycin and streptomycin.
abstract: Strains containing a series of restrictive and non-restrictive mutations in ribosomal protein S12 have been transformed with plasmids carrying the rrnB operon with mutations at positions 1409 and 1491 in 16S rRNA. The effects of the double-mutant constructs have been measured by growth rate, paromomycin and streptomycin sensitivity, resistance and dependence. The results demonstrate a functional interaction between the 1409-1491 region of rRNA and ribosomal protein S12.
authors.affiliation: Section of Biochemistry, Brown University, Providence, RI 02912.
authors.forename: M
authors.initials: M
authors.lastname: O'Connor
authors.forename: E A
authors.initials: EA
authors.lastname: De Stasio
authors.forename: A E
authors.initials: AE
authors.lastname: Dahlberg
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005838:Genotype; D009154:Mutation; D010303:Paromomycin / Q000494:pharmacology*; D010641:Phenotype; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D013307:Streptomycin / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D010303:Paromomycin; D013307:Streptomycin