Detection and identification modes for the highly sensitive and simultaneous determination of various biogenic amines by coulometric high-performance liquid chromatography.
Detection and identification modes for the rapid, selective, highly sensitive and simultaneous determination of catecholamines, indoleamines and related metabolites by high-performance liquid chromatography (HPLC) with series of coulometric working electrodes (CWE) were investigated. Five detection modes were examined: (1) oxidative single mode using a single CWE, (2) oxidative screen mode using a series of two CWE, (3) redox mode using a series of two CWE, (4) redox-reductive screen mode using a series of three CWE and (5) redox-reductive screen mode using a series of four CWE. For the highly sensitive detection of catechol compounds, oxidative single, redox and redox-reductive screen modes were suitable. Oxidative single and oxidative screen modes were better than the other modes for indole and o-methylated catechol compounds. For the selective detection of these compounds, however, the redox-reductive screen mode was best. The specific ratio obtained in HPLC with the redox or redox-reductive screen mode is useful as an index for identification purposes. These findings suggest that HPLC with the redox-reductive screen mode of detection is applicable to neuroscience studies.
Takeda
H
H
Department of Pharmacology, Tokyo Medical College, Japan.
Matsumiya
T
T
Shibuya
T
T
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000031:analogs & derivatives / Q000032:analysis; D006719:Homovanillic Acid / Q000032:analysis; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000032:analysis; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis
D001679:Biogenic Amines; D002395:Catecholamines; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D008734:Methoxyhydroxyphenylglycol; D006897:Hydroxyindoleacetic Acid; C001746:3-methoxytyramine; D004298:Dopamine; D006719:Homovanillic Acid