The putative role of members of the CEA-gene family (CEA, NCA an BGP) as ligands for the bacterial colonization of different human epithelial tissues.
Immobilized purified CEA (carcinoembryonic antigen), NCA (non-specific crossreacting antigen) and BGP I (biliary glycoprotein I) bind strains of E. coli (including EPEC) and some Salmonella species (including S. typhi, S. paratyphi A + B and S. java) while Shigella-, Yersinia- and Bacteroides- strains showed no adhesion. The binding was of high avidity, heat sensitive, dose dependent, saturable and nearly completely abolished in the presence of 10 mM alpha-methylmannoside. From inhibition studies with aromatic mannose compounds, it was suggested that in contrast to Salmonella strains E. coli strains exhibit a higher hydrophobicity in the binding region adjacent to the CEA-, NCA- and BGP-binding site. By further inhibition experiments it could be demonstrated that E. coli and Salmonella strains bind to high-mannose type oligosaccharides of these molecules via lectins on bacterial type I fimbriae. We conclude that the expression of products of this gene family on different human epithelial cells (colon-, bile canaliculi, uroepithel etc.) may function as ligands for bacterial colonization of epithelial tissues.
Leusch
H G
HG
Institut für Medizinische Immunologie, Medizinischen Fakultät, RWTH, Aachen.
Drzeniek
Z
Z
Hefta
S A
SA
Markos-Pusztai
Z
Z
Wagener
C
C
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000378:metabolism; D000951:Antigens, Neoplasm; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion; D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000378:metabolism*; D015815:Cell Adhesion Molecules; D004848:Epithelium / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008766:Methylmannosides / Q000494:pharmacology; D010047:Ovalbumin / Q000378:metabolism; D012475:Salmonella / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000951:Antigens, Neoplasm; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D008766:Methylmannosides; D000509:alpha-Fetoproteins; C008512:methylumbelliferyl-alpha-D-mannopyranoside; D006923:Hymecromone; C008466:methylmannoside; D010047:Ovalbumin