title: [Epitope specificity of hemagglutinating monoclonal anti-B antibodies].
abstract: Fine epitope specificity of ten monoclonal antibodies (MA) agglutinating red blood cells B was studied. Three methods were used: 1) inhibition of MA binding to natural antigen by synthetic oligosaccharides (OS) and their polyacrylamide conjugates, 2) direct MA binding to a series of synthetic OS-polyacrylamide conjugates differing in carbohydrate epitope density, 3) direct MA binding to the affinity sorbents. It is shown that all antibodies studied prefer trisaccharide B determinant Gal alpha 1-3(Fuc alpha 1-2) Gal independently of their ability to discriminate serological subgroups of B erythrocytes (B, Bweak, B3). The correlation of the MAs epitope specificity with their ability to agglutinate red blood cells B subgroups is discussed. Of an interest is that MAs which are able to agglutinate any B subgroups also bing the synthetic tetrasaccharide Gal alpha 1-3(Fuc alpha 1-2)Gal beta 1-3GalNAc, a B type 3 determinant.
authors.forename: O E
authors.initials: OE
authors.lastname: Galanina
authors.forename: E I
authors.initials: EI
authors.lastname: Deriugina
authors.forename: N I
authors.initials: NI
authors.lastname: Olovnikova
authors.forename: A E
authors.initials: AE
authors.lastname: Nosyrev
authors.forename: M I
authors.initials: MI
authors.lastname: Lapenkov
authors.forename: N B
authors.initials: NB
authors.lastname: Chekneva
authors.forename: T V
authors.initials: TV
authors.lastname: Zemlianukhina
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Korchagina
authors.forename: N V
authors.initials: NV
authors.lastname: Bovin
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006384:Hemagglutination; D006801:Humans; D008567:Membranes, Artificial; D009844:Oligosaccharides; D013379:Substrate Specificity
chemical_list: D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D008567:Membranes, Artificial; D009844:Oligosaccharides
vernacular_title: Epitopnaia spetsifichnost' gemaggliutiniruiushchikh monoklonal'nykh anti-B-antitel.