Purification and characterization of liver arylformamidase in rainbow trout and cattle.
1. Arylformamidases were purified from the liver of rainbow trout and cattle. 2. Optimal pH's were 7.7 and 8.0 for the fish and cattle enzyme, respectively. Both enzymes showed optimum temperature at 40 degrees C. Thermal and pH stability ranges and Arrhenius plots of the enzymes differed. 3. Km and molecular weight of the fish enzyme were determined to be 0.28 mM and 32,700, respectively, while those for the cattle enzyme were 0.78 mM and 42,5000, respectively.
Serrano
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AE
Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
Nagayama
F
F
D000818:Animals; D005558:Arylformamidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002845:Chromatography; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007737:Kynurenine / Q000031:analogs & derivatives / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D013045:Species Specificity; D013696:Temperature; D014337:Trout
C007772:N'-formylkynurenine; D007737:Kynurenine; D005558:Arylformamidase