Biological properties of a renotropic protein present in plasma of uninephrectomized rats.
The biological characteristics of a kidney growth factor (KGF) from uninephrectomized rat plasma have been studied. A crude preparation of this factor [Nephrol. Dial. Transplant. 4: 334-338, 1989] was further purified by hydrophobic interaction HPLC and gel filtration. KGF was found to be a heat- and trypsin-resistant protein. This factor stimulated dose-dependently DNA synthesis by the mouse kidney in vivo, and by either rat renal tubules or serum-deprived LLC-PK1 cells, in vitro. KGF also increased protein synthesis in these cells, in a dose-dependent manner. Moreover, KGF stimulated sodium uptake by these cells, associated with the maximal increase of protein synthesis. Our findings indicate that KGF is a potent renotropic protein which can play a key role in the renal compensatory growth after uninephrectomy.
Esbrit
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Laboratorio de la Unidad Metabólica, Fundación Jiménez Díaz-CSIC, Madrid, España.
García-Ocaña
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García-Cañero
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Manzano
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F
Jiménez-Clavero
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MA
D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D006133:Growth Substances / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D007668:Kidney / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D007684:Kidney Tubules / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D009392:Nephrectomy; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism
D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; C010320:renotropins; D004247:DNA; D012964:Sodium