Sequence-informative fragmentation of peptides up to a molecular weight of 4.6 kDa in plasma-desorption mass spectrometry.
The potential of plasma-desorption mass spectrometry (PDMS) in peptide sequencing is investigated. This paper shows that PDMS spectra recorded for longer times and using larger amounts of peptide than used for obtaining molecular weight information, provides sequence information on peptides of up to 4.5 kDa molecular weight. This approach strongly enhances the utility of PDMS for validation of the sequence of recombinant peptides.
Vorst
H J
HJ
Gist-brocades, R&D Central Analytical Department, Delft, The Netherlands.
van Tilborg
M W
MW
van Veelen
P A
PA
Tjaden
U R
UR
van der Greef
J
J
D000324:Adrenocorticotropic Hormone; D000595:Amino Acid Sequence; D000808:Angiotensinogen; D013007:Growth Hormone-Releasing Hormone; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013373:Substance P
D010446:Peptide Fragments; D000808:Angiotensinogen; D013373:Substance P; D000324:Adrenocorticotropic Hormone; D013007:Growth Hormone-Releasing Hormone