title: Expression of the large myelin-associated glycoprotein isoform during the development in the mouse peripheral nervous system.
abstract: The developmental maximum expression of the large myelin-associated glycoprotein isoform (L-MAG) protein prior to that of the small myelin-associated glycoprotein isoform (S-MAG) in both the central and peripheral nervous systems (CNS, PNS) in mice was shown by immunoblotting techniques using specific antibodies to the L-MAG protein and the S-MAG protein. Both the L-MAG protein and the S-MAG protein were expressed earlier in the PNS than in the CNS, which reflects earlier myelination in the PNS. The peak of the L-MAG protein concentration was 8 days in the sciatic nerve and 15 days in the brainstem. The concentration of the S-MAG protein in the sciatic nerve reached a peak at 15 days, whereas in the brainstem it increased rapidly between 15 and 20 days and gradually thereafter. Thus, the preceding maximum expression of the L-MAG during active myelination in the PNS demonstrated here as well as in the CNS strongly suggests an important role for L-MAG in myelin formation.
authors.affiliation: Department of Neurology, Niigata University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inuzuka
authors.forename: N
authors.initials: N
authors.lastname: Fujita
authors.forename: S
authors.initials: S
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Baba
authors.forename: R
authors.initials: R
authors.lastname: Nakano
authors.forename: H
authors.initials: H
authors.lastname: Ishiguro
authors.forename: T
authors.initials: T
authors.lastname: Miyatake
mesh_terms: D000375:Aging; D000818:Animals; D001933:Brain Stem / Q000254:growth & development* / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D015151:Immunoblotting; D051379:Mice; D008815:Mice, Inbred Strains; D009185:Myelin Proteins / Q000032:analysis / Q000235:genetics*; D018992:Myelin-Associated Glycoprotein; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D012584:Sciatic Nerve / Q000254:growth & development* / Q000502:physiology
chemical_list: D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D012333:RNA, Messenger