Suppression of messenger ribonucleic acid for glutathione peroxidase in chemically induced rat hepatocellular carcinoma and its biological significance.
Deviations of the enzyme activity, immunoreactivity and messenger ribonucleic acid (mRNA) levels of glutathione peroxidase (GSH-PO) in 3'-methyl-4-dimethylaminoazobenzene- induced hepatocellular carcinoma of the rat were investigated. Enzyme activities of GSH-PO were significantly lower in hepatocellular carcinomas than those in the normal control rat liver. Immunohistochemically, GSH-PO was strongly localized in normal hepatocytes, but was only faintly stained in hepatocellular carcinoma cells. Heterogeneous staining patterns of GSH-PO were observed among individual cancer cells. In Northern blot analysis, GSH-PO mRNA in the cancer tissue was decreased to two thirds of the level in normal hepatocytes. It was suggested that suppressed expression of GSH-PO in carcinogen-induced hepatocellular carcinomas occurred at the level of mRNA transcription.
Lertprasertsuke
N
N
Department of Pathology, School of Medicine, Tokai University, Kanagawa, Japan.
Shinoda
M
M
Takekoshi
S
S
Yoshimura
S
S
Watanabe
K
K
D000818:Animals; D015152:Blotting, Northern; D005979:Glutathione Peroxidase / Q000235:genetics*; D007150:Immunohistochemistry / Q000379:methods; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D008749:Methyldimethylaminoazobenzene; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D012333:RNA, Messenger; D008749:Methyldimethylaminoazobenzene; D005979:Glutathione Peroxidase