Covalent binding of bleomycin to concanavalin A and immunoglobulin G enhances the ability of the bleomycin-Fe(II) complex to destroy the erythrocyte membrane.
The antibiotic bleomycin was examined as a possible component of hybrid molecules composed of an address fragment and a generator of reactive oxygen species. The bleomycin-Fe(II) complex was found to destroy the erythrocyte membrane by generating reactive oxygen. The ability of antioxidants to slow down haemolysis points to a free-radical mechanism for this process. The protective effects of catalase and superoxide dismutase indicate that hydrogen peroxide and the superoxide radical formed on autoxidation of the complex are essential for membrane damage. Haemolytic activity is also exhibited by bleomycin-Fe(III) reduced in the NADPH-cytochrome P450 reductase reaction. The covalent binding of bleomycin to such address molecules as concanavalin A and antierythrocyte immunoglobulin G enhances the ability of the bleomycin-Fe(II) complex to destroy the plasma membrane of erythrocytes.
Voznesenskii
A I
AI
Second Moscow Medical Institute, Ministry of Health of the RSFSR.
Galanova YuV
Archakov
A I
AI
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001761:Bleomycin / Q000737:chemistry* / Q000494:pharmacology* / Q000031:analogs & derivatives; D002374:Catalase / Q000494:pharmacology; D003208:Concanavalin A / Q000737:chemistry*; D004910:Erythrocyte Membrane / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D007074:Immunoglobulin G; D007700:Kinetics; D051379:Mice; D008808:Mice, Inbred CBA; D011485:Protein Binding; D013482:Superoxide Dismutase / Q000494:pharmacology
D000975:Antioxidants; D007074:Immunoglobulin G; C023503:iron bleomycin; D003208:Concanavalin A; D001761:Bleomycin; D002374:Catalase; D013482:Superoxide Dismutase