[The effect of sorbitol on the determination of keto amines using a colorimetric method].
The presence of sorbitol in a concentration 11 mg/ml in a sample reduces by 68 percent the values of fructose glycine measured by colorimetry with thiobarbituric acid. Such sorbitol concentration has no effect on furfural production and on 5-hydroxymethylfurfural reaction with thiobarbituric acid. Measurements of glycolyzed hemoglobin have demonstrated that sorbitol in concentration 11 mg/ml completely inhibits hydrolysis. This fact should be borne in mind when measuring blood glycosylated hemoglobin by colorimetry with thiobarbituric acid and exclude sorbitol from patients' rations before measurements.
Kovaleva
G G
GG
Gorodetskiĭ
V K
VK
D003124:Colorimetry; D006442:Glycated Hemoglobin / Q000032:analysis*; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D013012:Sorbitol / Q000494:pharmacology*; D013858:Thiobarbiturates
D006442:Glycated Hemoglobin; D005998:Glycine; D007202:Indicators and Reagents; D013012:Sorbitol; D013858:Thiobarbiturates; C033188:fructosyl-glycine; C029684:thiobarbituric acid
Vliianie sorbita na opredelenie ketoaminov kolorimetricheskim metodom.