Identification and characterization of the hamster polyomavirus middle T antigen.
Hamster polyomavirus (HaPV) is associated with lymphoid and hair follicle tumors in Syrian hamsters. The early region of HaPV has the potential to encode three polypeptides (which are related to the mouse polyomavirus early proteins) and can transform fibroblasts in vitro. We identified the HaPV middle T antigen (HamT) as a 45-kDa protein. Like its murine counterpart, HamT was associated with serine/threonine phosphatase, phosphatidylinositol-3 kinase, and protein tyrosine kinase activities. However, whereas mouse middle T antigen associates predominantly with pp60c-src and pp62c-yes, HamT was associated with a different tyrosine kinase, p59fyn. The ability of HaPV to cause lymphoid tumors may therefore reside in its ability to associate with p59fyn, a potentially important tyrosine kinase in lymphocytes.
Courtneidge
S A
SA
Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Goutebroze
L
L
Cartwright
A
A
Heber
A
A
Scherneck
S
S
Feunteun
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis*; D002460:Cell Line; D006224:Cricetinae; D008647:Mesocricetus; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000276:immunology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000276:immunology; D051076:Proto-Oncogene Proteins c-fyn; D051575:Proto-Oncogene Proteins c-yes; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000276:immunology; D019061:src-Family Kinases
D000952:Antigens, Polyomavirus Transforming; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D051076:Proto-Oncogene Proteins c-fyn; D051575:Proto-Oncogene Proteins c-yes; D016392:Proto-Oncogene Proteins pp60(c-src); D019061:src-Family Kinases