title: [3'-Mercapto-3'-deoxythymidine-5'-triphosphate as a terminator of DNA synthesis catalyzed by RNA-dependent DNA-polymerases].
abstract: 3'-Mercapto-3'-deoxy-TTP was synthesized and tested as DNA chain terminator nucleotide for calf thymus alpha DNA polymerase, E. coli DNA polymerase I (Klenow fragment), terminal deoxyribonucleotidyltransferase (Bollum enzyme) and reverse transcriptase from AMV- and HIV-I-viruses. It was shown that the compound terminates DNA chain elongation by reverse transcriptases selectively and irreversibly. Other tested DNA polymerases do not use this nucleotide analogue as a substrate. 3'-Mercapto-3'-deoxythymidine was tested on lymphoblastoid T-cell line MT-4 with HIV-viruses and shown to suppress viruses as efficiently as 3'-azido-3'-deoxythymidine.
authors.forename: A A
authors.initials: AA
authors.lastname: Iuzhakov
authors.forename: Z G
authors.initials: ZG
authors.lastname: Chidzhavadze
authors.forename: P Sh
authors.initials: PSh
authors.lastname: Bibilashvili
authors.forename: A A
authors.initials: AA
authors.lastname: Kraevskiĭ
authors.forename: G A
authors.initials: GA
authors.lastname: Galegov
authors.forename: M N
authors.initials: MN
authors.lastname: Korneeva
authors.forename: D N
authors.initials: DN
authors.lastname: Posik
authors.forename: T Iu
authors.initials: TIu
authors.lastname: Kilesso
mesh_terms: D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001483:Base Sequence; D002384:Catalysis; D002417:Cattle; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects; D004256:DNA Polymerase I / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000201:enzymology; D015497:HIV-1 / Q000201:enzymology; D008969:Molecular Sequence Data; D018894:Reverse Transcriptase Inhibitors; D013942:Thymine Nucleotides / Q000494:pharmacology*
chemical_list: D018894:Reverse Transcriptase Inhibitors; D013942:Thymine Nucleotides; C070668:3'-mercapto-3'-deoxythymidine-5'-triphosphate; D004247:DNA; D004256:DNA Polymerase I
vernacular_title: 3'-Merkapto-3'-dezoksitimidin-5'-trifosfat kak terminator sinteza DNK, kataliziruemogo RNK-zavisimymi DNK-polimerazami.