title: Analysis of calcium activated chloride current fluctuations in Xenopus laevis oocytes.
abstract: Fluctuations of calcium activated chloride currents were investigated in oocytes of Xenopus laevis. The method of noise analysis and the model of chloride channels activation by calcium ions were used to estimate the chloride channels lifetime and the average frequency of current fluctuations, which depends on changes of cytoplasmic calcium concentration. This current fluctuations can be evoked by activation of cholinergic receptors or inhibition by Na3VO4 of plasma membrane Ca(2+)-ATPase. The average opening lifetime of chloride channels was approximately 100 ms. The frequency of fluctuations increased with the increasing extracellular calcium concentrations and external ACh concentrations. Caffeine in 2 mmol/l concentration changed the current fluctuations into oscillations with a period of about 18-20s. Ten mmol/l caffeine fully inhibited the oscillation activity.
authors.affiliation: Institute of Neurobiology, Slovak Academy of Sciences, Kosice.
authors.forename: T
authors.initials: T
authors.lastname: Kristian
authors.forename: M
authors.initials: M
authors.lastname: Kolaj
authors.forename: J
authors.initials: J
authors.lastname: Poledna
mesh_terms: D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D018118:Chloride Channels; D002712:Chlorides / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000378:metabolism*; D008954:Models, Biological; D009865:Oocytes / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D007473:Ion Channels; D008565:Membrane Proteins; D000252:Calcium-Transporting ATPases; D002118:Calcium