[Human lymphocyte transformation after immortalization by transfection with cytoplasmic DNA from mouse L cells of the cytokines IL-1alpha, IL-6 and TNF].
Human lymphocytes from peripheral blood were induced to proliferate indefinitely in vitro by transfection with cytoplasmic DNA isolated from transformed mouse L929 cells. Two cell lines analyzed proliferate in chemically defined, serum-free media without addition of cytokines. Using RIA and bioassays, the cells were found to secrete IL-1 alpha, IL-6, and TNF into the culture medium whereas no IL-2, IL-4, BCGF, CSF, or IFN-gamma were detected. Northern blot hybridizations revealed TNF alpha mRNA (1.5 kb) as well as TNF beta mRNA (1.4 kb) transcripts in RNA of the immortalized cells. Since the immortalized lymphoid cell lines neither form colonies in soft agar medium nor induce tumors after injection into immunodeficient nude mice, we suggest that these cytokines may be involved in autocrine growth stimulation of human lymphoid cell lines immortalized by transfection with cytoplasmic DNA from mouse L-cells.
Abken
H
H
Abteilung Molekulargenetik, Universität Bonn.
Fluck
J
J
Willecke
K
K
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics*; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D007739:L Cells / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D014158:Transcription, Genetic; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D004247:DNA
Menschliche Lymphozyten sezernieren nach Immortalisierung durch Transfektion einer zytoplasmatischen DNS aus Maus-L-Zellen die Zytokine IL-1 alpha, IL-6 und TNF.