title: All T15 Id-positive antibodies (but not the majority of VHT15+ antibodies) are produced by peritoneal CD5+ B lymphocytes.
abstract: After adult irradiation and reconstitution with autologous bone marrow, BALB/c and C.B20 mice no longer utilize the T15 Id in response to phosphorylcholine. T15 Id expression can be restored by transfers of peritoneal B cells or by FACS-purified CD5+ IgM+ lymphocytes (but not by T lymphocytes) from syngeneic donors. Using bone marrow and peritoneal cell donors that are congeneic for heavy and light chain allotypes, the exclusive origin of the T15 Id in peritoneal B cells was ascertained. These conclusions have been essentially confirmed by immunization with either anti-T15 Id or anti-VHT15 antibodies conjugated to lipopolysaccharide. Thus, the production of VHT15-positive antibodies continues at control levels in bone marrow-reconstituted animals while no T15 Id production can be stimulated even in this protocol of direct B cell stimulation. These results constitute the first formal demonstration of the exclusive production of and Id by CD5+ B-cells.
authors.affiliation: Département d'Immunologie (Université Pierre & Marie Curie and UA CNRS 359), Institut Pasteur, Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Masmoudi
authors.forename: T
authors.initials: T
authors.lastname: Mota-Santos
authors.forename: F
authors.initials: F
authors.lastname: Huetz
authors.forename: A
authors.initials: A
authors.lastname: Coutinho
authors.forename: P A
authors.initials: PA
authors.lastname: Cazenave
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation; D001402:B-Lymphocytes / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D018956:CD5 Antigens; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis*; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology; D010767:Phosphorylcholine / Q000276:immunology; D011828:Radiation Chimera
chemical_list: D000943:Antigens, Differentiation; D018956:CD5 Antigens; D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine