Identification of a binding site in the hepatitis B virus RNA pregenome for the viral Pol gene product.
The hepatitis B virus, although containing a DNA genome, replicates by reverse transcription of an RNA pregenome. The viral Pol gene encodes the reverse transcriptase which catalyzes viral DNA synthesis. To study the interaction of this protein with HBV RNA, the entire Pol gene product was expressed except its eight amino-terminal codons in Escherichia coli as fusion protein with beta-galactosidase. In the absence of competing nucleic acids full-length expression products were able to nonspecifically bind in vitro synthesized HBV RNAs of different polarity and length. However, if competed with an excess of unspecific RNA, only those HBV RNAs were bound which contained besides the direct repeats 1 and 2 nucleotide sequences downstream of direct repeat 1. The corresponding binding site was found to be located within the adjacent 134 nucleotides downstream of DR1. We conclude from our data that this region which is in part homologous to the U5 region of retroviral genomes may be important for the binding of the HBV Pol gene product to the viral pregenome.
Köchel
H G
HG
Center of Hygiene and Human Genetics of the University, Department of Medical Microbiology, Göttingen, Germany.
Kann
M
M
Thomssen
R
R
D001665:Binding Sites; D015153:Blotting, Western; D003001:Cloning, Molecular; D006515:Hepatitis B virus / Q000235:genetics*; D066298:In Vitro Techniques; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014779:Virus Replication
D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D012194:RNA-Directed DNA Polymerase