title: Structure of a ternary complex of an allosteric lactate dehydrogenase from Bacillus stearothermophilus at 2.5 A resolution.
abstract: We report the refined structure of a ternary complex of an allosterically activated lactate dehydrogenase, including the important active site loop. Eightfold non-crystallographic symmetry averaging was utilized to improve the density maps. Interactions between the protein and bound coenzyme and oxamate are described in relation to other studies using site-specific mutagenesis. Fructose 1,6-bisphosphate (FruP2) is bound to the enzyme across one of the 2-fold axes of the tetramer, with the two phosphate moieties interacting with two anion binding sites, one on each of two subunits, across this interface. However, because FruP2 binds at this special site, yet does not possess an internal 2-fold symmetry axis, the ligand is statistically disordered and binds to each site in two different orientations. Binding of FruP2 to the tetramer is signalled to the active site principally through two interactions with His188 and Arg173. His188 is connected to His195 (which binds the carbonyl group of the substrate) and Arg173 is connected to Arg171 (the residue that binds the carboxylate group of the substrate).
authors.affiliation: Department of Biochemistry, University of Leicester, U.K.
authors.forename: D B
authors.initials: DB
authors.lastname: Wigley
authors.forename: S J
authors.initials: SJ
authors.lastname: Gamblin
authors.forename: J P
authors.initials: JP
authors.lastname: Turkenburg
authors.forename: E J
authors.initials: EJ
authors.lastname: Dodson
authors.forename: K
authors.initials: K
authors.lastname: Piontek
authors.forename: H
authors.initials: H
authors.lastname: Muirhead
authors.forename: J J
authors.initials: JJ
authors.lastname: Holbrook
mesh_terms: D000494:Allosteric Regulation; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003461:Crystallography; D004252:DNA Mutational Analysis; D005636:Fructosephosphates / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D007770:L-Lactate Dehydrogenase / Q000648:ultrastructure*; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D010315:Particle Accelerators; D014961:X-Ray Diffraction
chemical_list: D005636:Fructosephosphates; D046911:Macromolecular Substances; D009243:NAD; D007770:L-Lactate Dehydrogenase