title: CD66 identifies a neutrophil-specific epitope within the hematopoietic system that is expressed by members of the carcinoembryonic antigen family of adhesion molecules.
abstract: Preliminary results from the IVth Leucocyte Culture Conference have classified the monoclonal antibody (MoAb), YTH 71.3.2, as CD66. Two other MoAbs, YPC 2/12.1 and CE6/2D3.1, share a common cellular specificity, reacting with cells of the neutrophil series and colonic epithelium. The YTH 71.3.2 and CE6/2D3.1 MoAbs both recognize a similar CD66 defined epitope that is distinct from that identified by YPC 2/12.1. By Western blotting, these antibodies react with different molecular species from cells of different lineages. The antibodies identify 50- to 55-Kd, 80- to 100-Kd, and 130- to 200-Kd components present in a semi-purified carcinoembryonic antigen (CEA) preparation from colonic adenocarcinomas and a 90- to 130-Kd molecule from HL-60 cells. With the colonic cell line, LS174T, YPC2/12.1 stains diffuse bands of 160 to 200 Kd and 90 to 130 Kd with equal intensity, whereas the binding of CE6/2D3.1 and YTH 71.3.2 is biased toward the lower molecular weight set of molecules. Remarkably, all three antibodies recognize CEA-related molecules. Defined analyses using HeLa cells transfected with CEA, NCA(NCA-50/90), and CGM6(NCA-95) cDNAs show that the three MoAbs identify CEA to varying degrees. While YTH 71.3.2 and CE6/2D3.1 also bind to NCA-50/90, YPC 2/12.1 recognizes an epitope expressed by both the NCA-50/90 and NCA-95 molecular species.
authors.affiliation: Imperial Cancer Research Fund, London, UK.
authors.forename: S M
authors.initials: SM
authors.lastname: Watt
authors.forename: G
authors.initials: G
authors.lastname: Sala-Newby
authors.forename: T
authors.initials: T
authors.lastname: Hoang
authors.forename: D J
authors.initials: DJ
authors.lastname: Gilmore
authors.forename: F
authors.initials: F
authors.lastname: Grunert
authors.forename: G
authors.initials: G
authors.lastname: Nagel
authors.forename: S J
authors.initials: SJ
authors.lastname: Murdoch
authors.forename: E
authors.initials: E
authors.lastname: Tchilian
authors.forename: E S
authors.initials: ES
authors.lastname: Lennox
authors.forename: H
authors.initials: H
authors.lastname: Waldmann
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D000943:Antigens, Differentiation / Q000032:analysis* / Q000276:immunology; D001667:Binding, Competitive; D015153:Blotting, Western; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D005434:Flow Cytometry; D006367:HeLa Cells; D006413:Hematopoietic System / Q000276:immunology*; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D000939:Epitopes