[The determination of free hemoglobin in blood plasma using the hemiglobincyanide method].
The authors have proved the possibility of using the hemiglobincyanide method for measurements of blood plasma free hemoglobin: free hemoglobin was measured in autoblood reinfused from the operation field by various methods (45-benzidine method, 3 parallel analyses in every study, and 45-hemiglobincyanide method, modified by increasing the amount of the tested material from 0.02 to 2.0 ml, also 3 parallel analyses per sample); a correction coefficient equal to 71.7 is suggested, and correction of the data according to a formula.
Savel'ev
O N
ON
Sukhorukov
V P
VP
Kiseleva
A V
AV
Koroleva
G A
GA
D001804:Blood Transfusion, Autologous; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D007432:Intraoperative Period; D008706:Methemoglobin / Q000031:analogs & derivatives*
D006454:Hemoglobins; D007202:Indicators and Reagents; C018958:hemiglobincyanide; D008706:Methemoglobin
Opredelenie svobodnogo gemoglobina plazmy krovi gemiglobintsianidnym metodom.