[Fusion of protoplasts of inactive variants of 2 producers of actinomycin C and the biosynthesis of an antibiotic of non- actinomycin nature].
Fusion of protoplasts of double auxotrophic mutants of spontaneous inactive variants of two cultures producing actinomycin C, i.e. Streptomyces chrysomalus 305 and Streptomyces sp. 26-115 induced by PEG-600 yielded a number of stable recombinants. One of the recombinants requiring proline for its growth was designated as recPro. Unlike its parent strains, it synthesized an antibiotic substance active against gram-positive bacteria and Saccharomyces cerevisiae. The nature of the substance is under investigation.
Orlova
T I
TI
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology; D003470:Culture Media; D003609:Dactinomycin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D066298:In Vitro Techniques; D009154:Mutation / Q000235:genetics; D011523:Protoplasts / Q000166:cytology / Q000378:metabolism*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D013302:Streptomyces / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003470:Culture Media; C030013:cactinomycin; D003609:Dactinomycin
Sliianie protoplastov neaktivnykh variantov dvukh produtsentov aktinomitsina C i biosintez antibiotika neaktinomitsinovoĭ prirody.