A new method for selective localization of flavan-3-ols in plant tissues involving glycolmethacrylate embedding and microwave irradiation.
A method for selective staining of flavan-3-ols in plant tissues fixed with glutaraldehyde is given. The use of glycolmethacrylate as embedding medium allows the sulphuric acid-containing staining solution to be heated without destroying the fine structure of the tissue. The distribution of flavan-3-ols and proanthocyanidins in different plant tissues is discussed.
Gutmann
M
M
Institut für Pflanzenbau, Lehrstuhl für Obstbau, Technische Universität München, Federal Republic of Germany.
Feucht
W
W
D000872:Anthocyanins / Q000032:analysis*; D005404:Fixatives; D005419:Flavonoids / Q000032:analysis*; D005976:Glutaral / Q000737:chemistry; D006652:Histological Techniques; D006358:Hot Temperature; D008872:Microwaves; D010944:Plants; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry; D044945:Proanthocyanidins; D013194:Staining and Labeling
D000872:Anthocyanins; D005404:Fixatives; D005419:Flavonoids; D044945:Proanthocyanidins; C013221:proanthocyanidin; D011102:Polyhydroxyethyl Methacrylate; D005976:Glutaral