[Cytoplasmic proteins in the uterine cervix].
The uterine cervical tissue was homogenized in STM buffer (0.25 M sucrose, 10 mM MgCl2, 10 mM Tris, pH 7.5). A soluble fraction with cytoplasmic proteins was obtained in the supernatant by centrifugation at 100,000 g for 40 minutes. According to the Davis method, there was almost no difference between the most abundant native cytoplasmic proteins (0.8/0.4/0.1/0.3) and the control serum proteins (0.8/0.1/0.4/0.3). Otherwise, according to the Laemmli method, the third and the fourth number of the "four-part numerical code" were strongly different for cytoplasmic proteins (67, 41, 186, 294) toward serum proteins (67, 41, 112, 235). Those data confirm Irwin's suggestion about the specificity of the "tissue codes".
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Zavoda za biologiju Medicinskog fakulteta Sveucilista u Zagrebu.
D002584:Cervix Uteri / Q000737:chemistry* / Q000648:ultrastructure; D003593:Cytoplasm / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D011506:Proteins / Q000032:analysis*
D011506:Proteins
Citoplazmatski proteini cerviksa uterusa.