Effect of endothelin-1 on the vascular smooth muscle cell cycle.
Rabbit vascular smooth muscle cells were cultured in various concentrations of endothelin-1 (ET-1) at 37 degrees C for 24 h. The vascular smooth muscle cell cycle distribution was determined by flow cytometry according to DNA content. In the control group, the mitotically active phase (S + G2 + M phase) of vascular smooth muscle cells was 11.94%. In ET-1-cultured smooth muscle cells, the mitotically active phase was 11.69% at a concentration of 1 pM ET-1, whereas the mitotically active phases were 18.98 and 26.91% at concentrations of 0.1 and 10 nM ET-1, respectively. The findings show that ET-1 significantly increased mitotic activity of cultured vascular smooth muscle cells.
Yu
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Institute of Geriatrics of Chinese PLA General Hospital, Beijing.
Yu
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CC
Wong
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Yuan
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D000818:Animals; D001011:Aorta / Q000166:cytology; D002453:Cell Cycle / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D016232:Endothelins / Q000494:pharmacology*; D005434:Flow Cytometry; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011817:Rabbits
D016232:Endothelins; D004247:DNA