title: [Rapid assay of hyaluronic acid in serum].
abstract: A method for measurement of hyaluronic acid (HA) level in serum was developed based on using "hyaluronic acid binding protein" (HABP)-coated polystyrene beads. After the beads and test serum being mixed, the mixture was incubated together with reaction buffer for 2 hours, and then the beads were washed. Subsequently, biotinylated HABP was added to the washed beads and incubated for 1 hour. Then peroxidase-conjugated avidin was added to the mixture and incubated again for 1 hour. After the beads being washed, a substrate solution was added to the washed beads and left for 1 hour. Then the reaction was stopped by adding 2N-H2SO4. The absorbance at 492 nm was recorded. The analytical range of HA in serum by this method was found to be between 10-800 micrograms/l, and the precision of the HA assay (CV%) was between 3.0-8.4 in the "with-in" assay (n = 10), and 4.8-8.9 in the "between" assay (n = 5). The analytical recovery of HA assay was between 92-115%. In this study, the results in screening of the serum HA level in RA patients (n = 107), OA patients (n = 16) and healthy subjects (n = 30) showed that the HA level of RA patients was demonstrated significantly higher than that of healthy subjects and OA patients.
authors.affiliation: Diagnostics Technology Labs, Chugai Pharmaceutical Co., Ltd., Tokyo.
authors.forename: T
authors.initials: T
authors.lastname: Kondo
authors.forename: K
authors.initials: K
authors.lastname: Chichibu
authors.forename: H
authors.initials: H
authors.lastname: Usuki
authors.forename: T
authors.initials: T
authors.lastname: Matsuura
authors.forename: S
authors.initials: S
authors.lastname: Shichijo
authors.forename: M M
authors.initials: MM
authors.lastname: Yokoyama
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000097:blood; D002352:Carrier Proteins; D006801:Humans; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid / Q000097:blood*; D008722:Methods; D008875:Middle Aged; D010003:Osteoarthritis / Q000097:blood
chemical_list: D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid