title: Toxic effects of alkyl-lysophospholipids on human bone marrow and de novo leukaemias. A short overview.
abstract: Alkyl-lysophospholipids (ALPs) are reported to have an antineoplastic activity against leukaemic cells. We have tested some halogen-containing ALPs from the Central Institute of Molecular Biology (H. Brachwitz) in comparison with racemic 1-ostadecyl-2-methyl-glycero-3-phosphocholine (ET-18-OCH3) (P.G. Munder, Max-Planck-Institut für Immunobiologie, Freiburg, FRG). We found freshly dissolved ALPs to be very toxic both to human bone marrow and to leukaemic cells of patients. ALP-incubation before cryopreservation is more toxic to bone marrow (but not to AML blasts) than after cryopreservation. All experiments to test the selectivity and to establish a purging protocol should be done using 1, remission marrow including a cryopreservation step and 2, blasts of de novo leukaemias instead of cell as sensitive as HL 60 to ALP-incubation. We found direct toxicity of ALPs to be not suitable for purging lines of bone marrow from patients in remission.
authors.affiliation: District Institute of Blood Transfusion, Leipzig, GDR.
authors.forename: M
authors.initials: M
authors.lastname: Wötzel
authors.forename: H
authors.initials: H
authors.lastname: Brachwitz
authors.forename: P
authors.initials: P
authors.lastname: Langen
authors.forename: V
authors.initials: V
authors.lastname: Thierbach
mesh_terms: D000478:Alkylation; D001752:Blast Crisis / Q000473:pathology; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015925:Cryopreservation; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000473:pathology*; D015473:Leukemia, Promyelocytic, Acute; D008246:Lysophospholipids / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
chemical_list: D008246:Lysophospholipids