title: Acceleration of the hemopoietic reconstitution in mice undergoing bone marrow transplantation by recombinant human granulocyte colony-stimulating factor.
abstract: We have investigated the effects of recombinant human granulocyte colony-stimulating factor (rG-CSF) on hemopoietic reconstitution after bone marrow transplantation (BMT) following lethal irradiation in mice. Mice received a daily administration of 10 micrograms/kg rG-CSF or control vehicle one through 21 days after BMT. Spleen colony-forming units (CFU-S), granulocyte-macrophage colony-forming units (CFU-GM), megakaryocyte colony-forming units (CFU-Meg), and erythroid burst-forming units (BFU-E) increased in both bone marrow and spleen of the rG-CSF-treated mice as compared with the control. This increase was evident during the administration period. In spite of the increase in the progenitor cells in bone marrow and spleen, only a recovery of neutrophils was accelerated in peripheral blood. Thus rG-CSF accelerated granulopoietic recovery in the BMT mice, with an enhanced recovery of the stem cells and the progenitors for erythrocytes and megakaryocytes. These results indicate the potential clinical usefulness of rG-CSF in the treatment of patients undergoing BMT.
authors.affiliation: Fuji-Gotemba Research Laboratories, Chugai Pharmaceutical Company, Shizuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tamura
authors.forename: T
authors.initials: T
authors.lastname: Yoshino
authors.forename: K
authors.initials: K
authors.lastname: Hattori
authors.forename: A
authors.initials: A
authors.lastname: Kawamura
authors.forename: H
authors.initials: H
authors.lastname: Nomura
authors.forename: N
authors.initials: N
authors.lastname: Imai
authors.forename: M
authors.initials: M
authors.lastname: Ono
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D003114:Colony-Forming Units Assay; D005269:Femur; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000502:physiology; D011994:Recombinant Proteins; D013154:Spleen / Q000502:physiology
chemical_list: D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor