[219 Zw(a) positive mothers of children with clinically suspected neonatal alloimmune thrombocytopenia].
The sera of 219 Zwa-positive mothers who gave birth to children with clinically suspected neonatal alloimmune thrombocytopenia (NAIT) were tested for platelet-reactive antibodies using the platelet adhesion immunofluorescence test and a glycoprotein-specific immunoassay (MAIPA). In 102 families a serological crossmatch of maternal serum against paternal platelets was performed. 12 sera contained platelet specific antibodies: Zwb (1), Bra (4), Bra and HLA (5), Baka and HLA (1), Sra (1); three further sera contained antibodies against blood group A (1), blood group B (1), blood group B and HLA (1). Anti-Bra was the most important antibody in this cohort. There is evidence that in certain cases antibodies against blood group antigens A or B may cause NAIT. Clinical data of NAIT duo to anti-Bra suggest a less severe bleeding tendency than in NAIT due to anti-Zwa. The HLA-DRw6 antigen was identified as an immunogenetic marker for the immunization against the Bra-antigen.
Kroll
H
H
Institut für Klinische Immunologie und Transfusionsmedizin, Justuts-Liebig-Universität Giessen.
Kiefel
V
V
Mueller-Eckhardt
G
G
Santoso
S
S
Mueller-Eckhardt
C
C
D000918:Antibody Specificity / Q000276:immunology; D016824:Antigens, Human Platelet; D001788:Blood Grouping and Crossmatching / Q000379:methods; D001792:Blood Platelets / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007231:Infant, Newborn; D039661:Integrin beta3; D007518:Isoantibodies / Q000032:analysis*; D007519:Isoantigens / Q000276:immunology*; D008431:Maternal-Fetal Exchange / Q000276:immunology; D011247:Pregnancy; D013921:Thrombocytopenia / Q000097:blood / Q000175:diagnosis*
C055973:5b alloantigen, human; D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D007074:Immunoglobulin G; D039661:Integrin beta3; D007518:Isoantibodies; D007519:Isoantigens
Untersuchung bei 219 Zwa-positiven Müttern von Kindern mit klinischem Verdacht einer neonatalen Alloimmunthrombozytopenie.