title: Secondary structural analysis of two recombinant murine proteins, interleukins 1 alpha and 1 beta: is infrared spectroscopy sufficient to assign structure?
abstract: The secondary structure for two murine recombinant proteins, interleukins 1 alpha and 1 beta (rmIL-1 alpha and -1 beta), has been analyzed by Fourier transform infrared (IR) spectroscopy and then compared to results obtained by X-ray diffraction, circular dichroism (CD), and nuclear magnetic resonance (NMR) spectroscopy. The IR results obtained here for rmIL-1 alpha and -1 beta suggested that their secondary structures consisted predominantly of beta-sheets or strands. However, the analysis also revealed a significant absorption band near 1656 cm-1, which is typically assigned to alpha-helical or random structures. When these same murine polypeptides were analyzed by CD, no evidence of alpha-helical structures was observed. Further, published X-ray diffraction and NMR studies characterizing the human forms of IL-1 alpha and -1 beta indicate the absence of alpha-helices and that the human proteins are composed mainly of beta-strands (i.e., greater than 55%), with approximately 24% of the amino acids involved in large loops connecting the strands. The murine IL-1 proteins, when compared to their respective human counterparts, each show greater than 80% sequence homology. Given this fact, the CD analyses, and the result that this IR band amounted to 21% of the overall integrated area, the absorption peak at 1656 cm-1 was attributed to the presence of large loops rather than to alpha-helical or random structures. Such a structural assignment appears reasonable and is totally consistent with the established existence of large loops in the human forms as well as in other proteins found to fold similarly (viz., human bFGF).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Central Research Division, Pfizer, Inc., Groton, Connecticut 06340.
authors.forename: C L
authors.initials: CL
authors.lastname: Wilder
authors.forename: A D
authors.initials: AD
authors.lastname: Friedrich
authors.forename: R O
authors.initials: RO
authors.lastname: Potts
authors.forename: G O
authors.initials: GO
authors.lastname: Daumy
authors.forename: M L
authors.initials: ML
authors.lastname: Francoeur
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D006801:Humans; D007375:Interleukin-1 / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid; D013055:Spectrophotometry, Infrared; D014961:X-Ray Diffraction
chemical_list: D007375:Interleukin-1; D011994:Recombinant Proteins