title: CD5+ B lymphocytes in high-risk islet cell antibody-positive and newly diagnosed IDDM subjects.
abstract: Human CD5+ B lymphocytes produce autoantibodies that bind to self- and exogenous antigens. Extremely high percentages of CD5+ B lymphocytes are present in the fetal and newborn periods, whereas they constitute only a minority of B lymphocytes in healthy adults. Increased percentages of circulating CD5+ lymphocytes have previously been demonstrated in several autoimmune diseases, including rheumatoid arthritis, progressive systemic sclerosis, Graves' disease, and Sjögren's syndrome. We measured the percentages of B lymphocytes that expressed the CD5 determinant in 93 control subjects (age range 1 day to 59 yr, mean +/- 22.6 +/- 17.7 yr), 17 subjects with newly diagnosed insulin-dependent diabetes mellitus (IDDM; range 5-29 yr, mean +/- SD 13 +/- 5.9 yr), 31 high-risk islet cell antibody (ICA)-positive nondiabetic subjects (range 4-45 yr, mean +/- SD 19.8 +/- 14.1 yr), and 13 subjects with IDDM of greater than 5 yr duration (range 10-43 yr, mean +/- SD 24.2 +/- 9.9 yr). We report that CD5+ B-lymphocyte percentages are strikingly age dependent in healthy control subjects, declining progressively from the newborn period to the middle-age years (r = -0.75, P = 0.0001). In ICA+ nondiabetic and recent-onset IDDM subjects less than 29 yr of age, the percentage of circulating CD5+ B lymphocytes fell within the 95% confidence intervals established for control subjects. However, the age-dependent rate of decline in the percentage of CD5+ B lymphocytes within the control range was slower in ICA+ and newly diagnosed IDDM subjects than in control subjects.
authors.affiliation: Department of Pediatrics, University of Florida College of Medicine, Gainesville.
authors.forename: D A
authors.initials: DA
authors.lastname: Schatz
authors.forename: F
authors.initials: F
authors.lastname: Lang
authors.forename: A B
authors.initials: AB
authors.lastname: Cantor
authors.forename: W J
authors.initials: WJ
authors.lastname: Riley
authors.forename: N K
authors.initials: NK
authors.lastname: Maclaren
authors.forename: J W
authors.initials: JW
authors.lastname: Sleasman
authors.forename: D J
authors.initials: DJ
authors.lastname: Barrett
mesh_terms: D000293:Adolescent; D000328:Adult; D000375:Aging / Q000276:immunology; D015703:Antigens, CD / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D001402:B-Lymphocytes / Q000276:immunology*; D018956:CD5 Antigens; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007515:Islets of Langerhans / Q000276:immunology*; D008875:Middle Aged; D012044:Regression Analysis
chemical_list: D015703:Antigens, CD; D001323:Autoantibodies; D018956:CD5 Antigens; C024211:islet cell antibody