Heterodimeric capping proteins constitute a highly conserved group of actin-binding proteins.
The two subunits of the heterodimeric protein cap32/34, an actin-binding protein, are encoded by separate single-copy genes. We have established the genomic structure of both genes. A sequence comparison of cap32/34 with capZ from chicken skeletal muscle and two partially known sequences from Saccharomyces cerevisiae and Xenopus laevis show that heterodimeric capping proteins belong to a highly conserved group of actin-binding proteins. This conclusion is supported by the cross-reaction of polyclonal antibodies against cap32 and cap34 with proteins from lower and higher eukaryotes. In addition, a system is presented that allows the expression of truncated cap34 polypeptides under the control of the cap34 promoter.
Hartmann
H
H
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Schleicher
M
M
Noegel
A A
AA
D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002645:Chickens; D003429:Cross Reactions; D004023:Dictyostelium / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D015800:Protozoan Proteins; D012313:RNA / Q000032:analysis; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic; D014982:Xenopus laevis
D000199:Actins; D005656:Fungal Proteins; D008840:Microfilament Proteins; D015800:Protozoan Proteins; C069272:cap32-34 protein, Dictyostelium; D012313:RNA