New in vitro line from a human (B) non-Hodgkin lymphoma.
An in vitro cell line (HT 58) has been established from a human (B) NHL xenograft. The lymphoma cells in culture retained their lymphoblastic appearance, DNA-content, IgM/lambda monoclonality and many immunophenotypic markers. The clonal chromosomal abnormalities involved the chromosomes 1, 2, 3 and 14. The cells expressed and produced chondroitin sulfate proteoglycans identified with mAbs that were raised against human articular cartilage CSPG. The cells also released IgM into the medium as well as substances that stimulated the proliferation of activated normal peripheral B-cells.
Kopper
L
L
1st Institute of Pathology, Semmelweis Medical University, Budapest, Hungary.
Bankfalvi
A
A
Mihalik
R
R
Nagy
P
P
Fulop
C
C
Sarmay
G
G
D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure; D002455:Cell Division; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D046508:Culture Techniques / Q000379:methods; D004273:DNA, Neoplasm / Q000032:analysis; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007621:Karyotyping; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D004273:DNA, Neoplasm; D000939:Epitopes