Neurochordins of higher vertebrates: similarities in P-epitope-bearing polypeptide composition and a study of P-epitope expression in cultured neural tissue cells.
Neurochordins are a family of high Mr P-epitope-bearing neural tissue glycoproteins. Comparison of SDS-agarose electrophoretic patterns of extracts from human, rat, mouse and chicken brain after immunostaining of blots with an anti-P-epitope MAb At5 demonstrated that in each case neurochordins from the A, B and C group were present. A similar result was obtained when polyclonal serum against total human neurochordin was used. The data favours the idea of high evolutional conservatism of neurochordins of higher vertebrate species. Expression of the P-epitope on glial cells in culture was studied. Strong immunostaining of oligodendrocytes with MAb At5 and the absence of this staining in astrocytes and Schwann cells make it possible to use At5 for identification of cell types in tissue culture experiments as well as for differential diagnostics of brain tumours.
Preobrazhensky
A A
AA
A.N. Bach Institute of Biochemistry, USSR Academy of Sciences, Moscow.
Khalansky
A S
AS
Kozlova
M V
MV
D000818:Animals; D000911:Antibodies, Monoclonal; D005075:Biological Evolution; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry; D002478:Cells, Cultured; D002645:Chickens; D004587:Electrophoresis, Agar Gel; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D009457:Neuroglia / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology; D051381:Rats; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins