Reinvestigation of phosphorylation of tRNA in Escherichia coli.
This report shows the results of the reinvestigation of tRNA phosphorylation in E. coli. The phosphorylation did not occur on suppressor seryl-tRNA but occurred on other tRNA species. The activity of tRNA phosphorylation was found in E. coli extracts and partially purified. On DEAE-Sephadex A50 and PAGE gel, the phosphorylated-tRNA showed a pattern different from that the natural suppressor serine tRNA.
Mizutani
T
T
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Kurata
H
H
D002852:Chromatography, Ion Exchange; D004926:Escherichia coli / Q000235:genetics*; D010766:Phosphorylation; D012329:RNA, Bacterial / Q000378:metabolism; D012343:RNA, Transfer / Q000302:isolation & purification / Q000378:metabolism*; D012352:RNA, Transfer, Glu / Q000378:metabolism; D012357:RNA, Transfer, Lys / Q000378:metabolism; D012362:RNA, Transfer, Ser / Q000378:metabolism
D012329:RNA, Bacterial; D012352:RNA, Transfer, Glu; D012357:RNA, Transfer, Lys; D012362:RNA, Transfer, Ser; D012343:RNA, Transfer