[The structure and composition of the complex that provides the adhesion of the cellular layers during the morphogenesis of the human embryonic adenohypophysis].
The area of contact between adenohypophysis and diencephalon rudiments of human embryos (5-7 weeks of development) was studied using immunohistochemistry and electron microscopy. Basement membranes of Rathke's pouch ectoderm and of diencephalon bottom neuroectoderm are connected by means of a complex consisting of thin fibrous material and collagen-like fibrils. After Ca+2 and Mg+2 ions removal, this complex with basement membranes was separated from epithelial layers. The material in the contact area differed in its composition from the basement membranes covering the adenohypophysis and diencephalon rudiments by the high content of tenascin and the absence of EDB-fibronectin. Other basement membrane components (collagen IV, heparan-sulphate proteoglycans, entactin and laminin) were also present in this area. Tenascin accumulation was also found in Rathke's pouch epithelium and cavity.
Bozhok
Iu M
IuM
Kozyritskiĭ
V G
VG
Zelinskaia
A V
AV
D000911:Antibodies, Monoclonal; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism / Q000648:ultrastructure*; D015816:Cell Adhesion Molecules, Neuronal / Q000276:immunology / Q000378:metabolism; D004027:Diencephalon / Q000196:embryology / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000276:immunology / Q000378:metabolism; D005353:Fibronectins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009024:Morphogenesis; D010503:Periodic Acid-Schiff Reaction; D010903:Pituitary Gland, Anterior / Q000196:embryology* / Q000378:metabolism; D019063:Tenascin
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D019063:Tenascin
Struktura i sostav kompleksa, obespechivaiushchego adgeziiu kletochnykh plastov v khode morfogeneza adenogipofiza zarodysheĭ cheloveka.