Quantification and partial characterisation of regulatory peptides in the liver fluke, Fasciola hepatica, from different mammalian hosts.
1. Extracts of the liver fluke, Fasciola hepatica from three different hosts (cow, sheep, rat) have been subjected to radioimmunoassay using antisera to 6 mammalian regulatory peptides. 2. Immunoreactivity was measured to pancreatic polypeptide, substance P, peptide histidine isoleucine and gastrin-releasing peptide. Levels of each peptide varied considerably in flukes from different hosts. 3. Reverse-phase HPLC of rat and sheep fluke extracts revealed three molecular forms of tachykinin immunoreactivity and single peaks of pancreatic polypeptide and peptide histidine isoleucine immunoreactivity. No GRP-immunoreactivity was detected by RIA of HPLC fractions.
Magee
R M
RM
School of Biology and Biochemistry, Queen's University, Belfast, Northern Ireland.
Fairweather
I
I
Shaw
C
C
McKillop
J M
JM
Montgomery
W I
WI
Johnston
C F
CF
Halton
D W
DW
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D002851:Chromatography, High Pressure Liquid; D005210:Fasciola hepatica / Q000032:analysis*; D005211:Fascioliasis / Q000469:parasitology / Q000662:veterinary*; D019886:Gastrin-Releasing Peptide; D009479:Neuropeptides / Q000032:analysis*; D010191:Pancreatic Polypeptide / Q000032:analysis; D010451:Peptide PHI / Q000032:analysis; D010455:Peptides / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*; D013373:Substance P / Q000032:analysis; D015320:Tachykinins / Q000032:analysis
D009479:Neuropeptides; D010451:Peptide PHI; D010455:Peptides; D015320:Tachykinins; D013373:Substance P; D010191:Pancreatic Polypeptide; D019886:Gastrin-Releasing Peptide