Macrophages produce nitrite, nitrate and nitrosamines after addition of catalase.
Mouse macrophages produced nitrite and N-nitrosomorpholine after incubation with catalase. A macrophage cell line, J774.1 (1 x 10(6) cells/ml), was incubated with catalase (500 U/ml) and morpholine (5 mM); after 48 h incubation at 37 degrees C, macrophages produced nitrite (100 microM) and N-nitrosomorpholine (1 microM). Stimulation of J774.1 cells with catalase enhanced interleukin-1 production and tumour-killing activity against mastocytoma P815 cells. Flow cytometric analysis showed that catalase was bound to the surface of the macrophages.
Miwa
M
M
National Food Research Institute, Ibaraki, Japan.
Watanabe
M
M
Nishida
T
T
Shinohara
K
K
D000818:Animals; D002374:Catalase / Q000494:pharmacology*; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D007372:Interferons / Q000494:pharmacology; D007375:Interleukin-1 / Q000032:analysis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D009602:Nitrosamines / Q000378:metabolism*
D007375:Interleukin-1; D008070:Lipopolysaccharides; D009566:Nitrates; D009573:Nitrites; D009602:Nitrosamines; D007372:Interferons; D002374:Catalase