Fast isolation of recombinant baculovirus by antibody screening.
The use of a rapid and efficient scheme for the detection and purification of recombinant baculoviruses is described. The method is based on the detection of foreign proteins in cellular lysates of baculovirus-infected insect cells by antibody screening. The recombinant virus is purified by repeated serial dilutions. The method allows the identification and purification of recombinant viruses within two to three weeks.
Manns
A
A
Department of Chemistry, Max-Planck-Institute for Experimental Medicine, Göttingen, Germany.
Grosse
F
F
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004279:DNA, Viral; D015870:Gene Expression; D015751:Genes, pol; D015497:HIV-1 / Q000201:enzymology / Q000235:genetics; D015151:Immunoblotting / Q000379:methods*; D009036:Moths / Q000382:microbiology; D012194:RNA-Directed DNA Polymerase / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral; D004274:DNA, Recombinant; D004279:DNA, Viral; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase