[Localization of an antigenic determinant of recombinant interleukin-2, recognized by monoclonal antibody 13B1].
We suggest a simple approach to localization of antigenic determinants for the monoclonal antibody 13B1 raised against recombinant human interleukin-2. The approach is based on the limited trypsin proteolysis, peptide separation by the O'Farrell method and identification of the peptides, interacting with monoclonal antibodies, and comparison of the charge and length of these peptides with the corresponding values of theoretically possible peptides.
Skrivelis
V A
VA
Bundulis
Iu P
IuP
Paniutich
A V
AV
Voĭtenok
N N
NN
Lunev
V E
VE
Nesmeianov
V A
VA
Tsimanis
A Iu
AIu
Gren
E Ia
EIa
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology*; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D014357:Trypsin / Q000737:chemistry
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007376:Interleukin-2; D011994:Recombinant Proteins; D014357:Trypsin
Lokalizatsiia antigennoĭ determinanty rekombinantnogo interleĭkina-2, uznavaemoĭ monoklonal'nymi antitelami 13B1.