The reaction of ferric- and ferrous salts with bleomycin.
1. A comparative study shows that ferrous ions give a much better yield of Fe(III)-bleomycin than ferric ions, when iron salt is added to bleomycin in a buffer solution (pH 7.2). 2. The amount of Fe(III)-bleomycin formed after addition of ferric ions was markedly increased in the presence of ferric ion binding compounds (BSA, citrate) or reducing agents (ascorbate, cysteine).
Løvstad
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RA
Institute of Medical Biochemistry, University of Oslo, Norway.
D001205:Ascorbic Acid / Q000494:pharmacology; D001761:Bleomycin / Q000378:metabolism*; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D003545:Cysteine / Q000494:pharmacology; D005290:Ferric Compounds / Q000378:metabolism*; D005296:Ferrous Compounds / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013053:Spectrophotometry
D002951:Citrates; D005290:Ferric Compounds; D005296:Ferrous Compounds; D001761:Bleomycin; D012710:Serum Albumin, Bovine; D019343:Citric Acid; D003545:Cysteine; D001205:Ascorbic Acid