In vivo effects of anti-idiotype on Plasmodium chabaudi infection in mice.
A polyclonal anti-idiotype was raised in rabbits following immunization with a murine monoclonal antibody which recognized a 250,000 MW antigen of Plasmodium chabaudi-infected erythrocytes. The monoclonal antibody, NIMP M23 (clone 3,) has been shown to protect mice against homologous parasite challenge. Following purification, the anti-idiotype was shown to bind only the immunizing idiotype and to recognize antigen-binding site-associated anti-idiotype. Mice primed with anti-idiotype and challenged with live parasites had an altered course of infection, with significant reduction in their peak parasitaemia levels. Anti-idiotype priming did not induce an antigen-reactive antibody response in vivo but a population of T cells capable of proliferating in vitro to P. chaubaudi-infected red cells was stimulated. These data are discussed in the context of possible idiotypic interaction in murine malaria.
Moore
M J
MJ
Department of Immunology, University College and Middlesex School of Medicine, London, U.K.
Hay
F C
FC
Wood
J
J
Brown
K N
KN
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000953:Antigens, Protozoan / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D008213:Lymphocyte Activation; D008288:Malaria / Q000276:immunology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D013601:T-Lymphocytes / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000953:Antigens, Protozoan; D000939:Epitopes