Transcriptional regulation of murine NADP(+)-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase-synthetase.
The cytosolic NADP(+)-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase is ubiquitously expressed in all mouse tissues and cell lines examined. Northern analyses of the RNA indicated that there is an extensive variation in the levels of mRNA in different tissues. However, the gene is refractory to induction by serum, phorbol esters or growth factors in cultured fibroblasts. The mRNA of the NADP(+)-dependent trifunctional enzyme is stabilized post-transcriptionally by insulin-like growth factor-1.
Peri
K G
KG
Department of Biochemistry, McGill University, Montréal, Québec, Canada.
MacKenzie
R E
RE
D016475:3T3 Cells; D000619:Aminohydrolases / Q000235:genetics*; D000818:Animals; D005574:Formate-Tetrahydrofolate Ligase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000494:pharmacology; D009097:Multienzyme Complexes / Q000235:genetics*; D009249:NADP / Q000378:metabolism; D009928:Organ Specificity; D011061:Poly A / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic
D008934:Mitogens; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D011061:Poly A; D009249:NADP; D012313:RNA; C021836:formyl-methenyl-methylenetetrahydrofolate synthetase; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D000619:Aminohydrolases; D005574:Formate-Tetrahydrofolate Ligase