TSH, IGF-1 and activated ras protein induce DNA synthesis in cultured thyroid cells.
TSH, IGF-1 and cellular ras genes have been proposed to function in thyroid cell transformation. Using cultured follicular cells, we demonstrate that TSH, IGF-1 and microinjected activated ras protein individually stimulate DNA synthesis. TSH-stimulated pathways include Gs at the plasma membrane and cyclic AMP response elements in the nucleus. The pathways and nuclear targets of IGF-1 and ras action appear at least partially distinct from those used by TSH.
Meinkoth
J L
JL
Department of Medicine, University of California, San Diego School of Medicine, La Jolla 92093-0602.
Dela Cruz
J
J
Burrow
G N
GN
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D004789:Enzyme Activation / Q000187:drug effects; D019204:GTP-Binding Proteins / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015290:Second Messenger Systems; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000378:metabolism
D015124:8-Bromo Cyclic Adenosine Monophosphate; D007334:Insulin-Like Growth Factor I; D013972:Thyrotropin; D004247:DNA; D000242:Cyclic AMP; D011494:Protein Kinases; D001616:beta-Galactosidase; D019204:GTP-Binding Proteins; D016283:Proto-Oncogene Proteins p21(ras)