The induction of specific-locus mutations with N-propyl-N-nitrosourea in stem-cell spermatogonia of mice.
A specific-locus test to determine the effect of N-propyl-N-nitrosourea (PNU) on the stem-cell spermatogonia of mice has been performed. Male wild-type mice (C3H/He) were treated with an intraperitoneal injection of 200 mg/kg [corrected] of PNU. Eight weeks after the injections, the males were mated with tester stock females (PW), homozygous for 6 visible recessive genes. Twelve mutants among 8605 offspring were observed. The mutation frequency with PNU was calculated to be 23.2 x 10(-5)/locus/gamete, showing a significant difference from that of the non-treated control. The mutations were all heritable and half of them were viable in homozygous condition. The mutation frequency with PNU was about one-third of that with N-ethyl-N-nitrosourea, a highly potent mutagen for mouse stem-cell spermatogonia.
Murota
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T
Laboratory of Genetic Toxicology, Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan.
Shibuya
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D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D005038:Ethylnitrosourea / Q000633:toxicity; D006720:Homozygote; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009607:Nitrosourea Compounds / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D012016:Reference Values; D013093:Spermatogonia / Q000187:drug effects* / Q000473:pathology; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects / Q000473:pathology
D002273:Carcinogens; D009153:Mutagens; D009607:Nitrosourea Compounds; C020796:propylnitrosourea; D005038:Ethylnitrosourea