title: Detection of common polysaccharide antigen of Pseudomonas aeruginosa with O-antiserum to Pseudomonas cerasi.
abstract: The identity of the structures of common polysaccharide antigen (CPA) of Pseudomonas aeruginosa and O-antigen of Pseudomonas cerasi was used for immunochemical study of polysaccharide antigens of seven immunotypes (IT) of P. aeruginosa. ELISA performed with O-antiserum to P. cerasi showed that CPA is present in all seven ITs in different amounts. In SDS-PAGE this antigen behaves as a lipopolysaccharide (LPS) and is detected by immunoblotting technique in five of seven ITs.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow.
authors.forename: N A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: A
authors.initials: A
authors.lastname: Romanowska
authors.forename: E
authors.initials: E
authors.lastname: Romanowska
authors.forename: T A
authors.initials: TA
authors.lastname: Makarenko
authors.forename: L S
authors.initials: LS
authors.lastname: Edvabnaya
authors.forename: E S
authors.initials: ES
authors.lastname: Stanislavsky
authors.forename: G M
authors.initials: GM
authors.lastname: Zdorovenko
authors.forename: L M
authors.initials: LM
authors.lastname: Yakovleva
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera; D008969:Molecular Sequence Data; D019081:O Antigens; D011549:Pseudomonas / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011817:Rabbits; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera; D019081:O Antigens