Immunotherapeutic potential in guinea-pig tumor model of deoxyribonucleic acid from Mycobacterium bovis BCG complexed with poly-L-lysine and carboxymethylcellulose.
In vivo antitumor activity of a deoxyribonucleic acid fraction obtained from Mycobacterium bovis BCG (named MY-1) increased when it was complexed with poly-L-lysine (poly LL) solubilized by addition of carboxymethylcellulose (CMC). The complex of MY-1 and poly LL/CMC induced interferon in vivo at a low dose of MY-1 which alone exerted no IFN induction. With Line 10 hepatoma (L10) which is syngeneic with strain 2 guinea pigs, it was demonstrated that repeated intralesional injections of the complex resulted in delay of tumor growth and complete cure of animals from L10 tumor inoculated. Similar treatment of the animals with the same amount of MY-1 or poly LL/CMC alone had little therapeutic effect on the tumor growth.
Kataoka
T
T
Department of Cellular Immunology, Tokyo.
Yamamoto
S
S
Yamamoto
T
T
Tokunaga
T
T
D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000008:administration & dosage*; D004269:DNA, Bacterial / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D006168:Guinea Pigs; D007167:Immunotherapy; D007372:Interferons / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000032:analysis*; D009374:Neoplasms, Experimental / Q000628:therapy*; D011107:Polylysine / Q000008:administration & dosage*
D004269:DNA, Bacterial; D011107:Polylysine; D007372:Interferons; D002266:Carboxymethylcellulose Sodium