Intrathoracic accumulation of 111In-labeled neutrophils in guinea pigs in response to PAF.
The intrathoracic content of neutrophils, labeled with 111In-oxine has been measured in the anesthetized guinea pig by using an automated isotope-monitoring system. Intravenous infusion of platelet-activating factor (PAF; 5.6, 10, or 18 ng.kg-1.min-1 over 5 min) caused a dose-related abrupt intrathoracic accumulation of neutrophils, which dispersed from the thorax within 20 min. Repetition of this procedure after 1 h gave responses of comparable magnitude and duration. Anti-platelet antiserum pretreatment did not influence the response of neutrophils to PAF. Iloprost infusion (10 ng.kg-1.min-1 over 15 min) did not affect the response of neutrophils to PAF, whereas accumulation of radiolabeled platelets in the lung was totally suppressed by this dose. Intrathoracic accumulation of neutrophils in response to PAF can be considered to be independent of platelet activation.
Hultkvist-Bengtsson
U
U
Preclinical Research, Sandoz, Basel, Switzerland.
Anderson
G P
GP
Morley
J
J
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D006168:Guinea Pigs; D016285:Iloprost / Q000494:pharmacology; D007700:Kinetics; D008168:Lung / Q000166:cytology; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013909:Thorax
D010972:Platelet Activating Factor; D016285:Iloprost