Use of chromatography for the preparation of homologous tetanus antitoxin.
To obtain immunoglobulin of selected action with a higher titre, techniques for isolating antibodies from a donor's normal immunoglobulin by means of affinity chromatography were used. To obtain an affinity sorbent, tetanus toxoid was used as a ligand. Using an immunosorbent based on the purified ligand, tetanus antibodies were prepared with a specific activity of 65 IU/mg. According to gel-permeation chromatography, the tetanus antibodies obtained were represented mainly by IgG monomer (greater than 90%). The anticomplementary activity of the preparations obtained, being at least 11 mg of protein per 2CH50, does not rule out the possibility of intravenous administration of tetanus antibodies.
Alyoshkin
V A
VA
G.N. Gabrichevsky Research Institute of Epidemiology and Microbiology, Moscow, U.S.S.R.
Borisova
V N
VN
Ponomaryova
A M
AM
Popkov MYu
D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D008024:Ligands; D013743:Tetanus Antitoxin / Q000302:isolation & purification*; D013744:Tetanus Toxin; D005719:gamma-Globulins / Q000032:analysis
D008024:Ligands; D013743:Tetanus Antitoxin; D013744:Tetanus Toxin; D005719:gamma-Globulins