Thyroid hormone receptor and receptor-related RNA levels in developing rat brain.
The proto-oncogene c-erbA and its analogues are genes that encode thyroid hormone receptors. In rats, at least two loci have been identified by their homology to c-erbA. Each locus can produce at least two distinct mRNA species via RNA processing. However, one of those transcripts, r-erbA alpha-2, does not yield a triiodothyronine (T3)-binding protein when translated in vitro. Proper development of the rat brain is thyroid hormone-dependent during the perinatal period and there is a documented increase in brin nuclear T3-binding capacity during that time. By hybridizing cDNA probes specific to various rat erbA-like transcripts with RNA extracted from developing rat brain, we sought changes in thyroid hormone receptor mRNA levels that correspond with changes in T3-binding capacity in rat brain during the perinatal period. Three mRNA of the erbA family showed variations in relative abundance during brain development. Oddly, r-erbA alpha-2, a variant that does not code for a T3-binding protein, was the most abundant erbA-like RNA to correlate with the reported changes in T3-binding capacity. The message for rat r-erbA alpha-1 that encodes a functional T3 receptor also varies with T3-binding capacity but at a level that is at least 8-fold lower than the r-erbA alpha-2 message. The level of rat erbA beta-1 message also varies in rat brain during the perinatal period but not in a way that correlates with changes in T3-binding capacity.
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Department of Pediatrics, UCLA Medical Center, Torrance 90509.
Fisher
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D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011988:Receptors, Thyroid Hormone / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D011988:Receptors, Thyroid Hormone; D012313:RNA