Ion transport in epithelial cells from the bovine trachea in culture: effects of dexamethasone and aldosterone.
The ion transport properties of epithelial cells from the bovine trachea grown in culture were investigated. The cellular uptake of 22Na+, 36Cl- and 86Rb+ was measured and the sensitivity of ion uptake to amiloride and bumetanide revealed the presence of the transport pathways that exist in the native tissue. The action of corticosteroids, dexamethasone and aldosterone (0.1 or 1.0 microM), was studied by measuring the short-circuit current and the uptake of Na+ and Cl- following a treatment of 18 h, 2 or 8 days. The results showed that, in each situation, dexamethasone enhanced markedly the amiloride-sensitive current and Na+ uptake. In contrast, aldosterone exerted no detectable effect on the ion transport and electrical properties.
Lehmann
C
C
Institut de Physiologie, Université de Fribourg, Suisse.
Durand
J
J
D000042:Absorption / Q000187:drug effects; D000450:Aldosterone / Q000494:pharmacology*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002034:Bumetanide / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D002713:Chlorine / Q000493:pharmacokinetics; D003907:Dexamethasone / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D007477:Ions; D008353:Mannitol / Q000378:metabolism; D011868:Radioisotopes; D012414:Rubidium Radioisotopes; D012979:Sodium Radioisotopes; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism
D007477:Ions; D011868:Radioisotopes; D012414:Rubidium Radioisotopes; D012979:Sodium Radioisotopes; D002034:Bumetanide; D008353:Mannitol; D000450:Aldosterone; D002713:Chlorine; D000584:Amiloride; D003907:Dexamethasone