[Double immunoenzymatic and two-color flow cytometric analyses of tonsillar T-cell subsets].
Distribution of various T-cell subsets in the palatine tonsil was investigated by two-color flow cytometry and double immunoenzymatic stain. Tonsillar lymphocytes contained many (about 20%) helper T (CD4+ Leu8-) cells and few (only 1%) suppressor T (CD8+ CD11b+) cells. In interfollicular area, each T-cell subset, i.e., helper, helper-inducer (CD4+ CD29+), suppressor-inducer (CD4+ CD45RA+), cytotoxic (CD8+ CD11b-), and suppressor, was identified by double immunoenzymatic labeling technique using alkaline phosphatase and peroxidase. On the other hand, only two T-cell subsets, helper, and cytotoxic T-cell subpopulations, were recognized in germial center. These results indicate that double immunoenzymatic stain as well as two-color flow cytometry gives us useful informations in terms of tonsillar T-cell subsets.
Ryan
Y
Y
Department of Otolaryngology, Sapporo Medical College, Sapporo.
Harabuchi
Y
Y
Kataura
A
A
D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D014066:Palatine Tonsil / Q000166:cytology*; D013194:Staining and Labeling; D016176:T-Lymphocyte Subsets