A method for the rapid identification of T lymphocyte epitopes.
Mounting interest in host immunity and understanding the pathogenesis of disease has focused attention on the identification of T lymphocyte epitopes within various antigens. This effort has proven troublesome because of time and financial constraints. We have developed a cost-effective technique that allows for the rapid identification of these antigenic sequences within proteins of interest. Using a panel of in vitro synthesized peptides and a CD4+ bovine T lymphocyte cell line, we have identified a single epitope on a herpesviral envelope glycoprotein. In the future, this technology should allow epitope mapping to be more practical for a large number of antigens.
Leary
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TP
Department of Veterinary Sciences, University of Wisconsin-Madison 53706.
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D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D000939:Epitopes; D006564:Herpesviridae / Q000276:immunology*; D011817:Rabbits; D014759:Viral Envelope Proteins / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins