Immunohistochemical localization of tenascin and fibronectin in the dentine and gingiva of Canis familiaris.
Specific, well-characterized antisera to the extracellular matrix glycoprotein tenascin were used. Immunoreactivity was detected in association with dentinal tubules; it was particularly prominent in the tooth crown, and was stronger within matrix than within predentine. In contrast, there was no anti-fibronectin staining in dentinal tubules. In periodontium, anti-tenascin immunoreactivity was stronger in the oral and sulcular gingival epithelia than in the underlying connective tissue, in contrast to the strong staining of connective tissue by anti-fibronectin. The appearance of tenascin immunoreactivity in gingival epithelia indicates that this protein is not exclusively a component of mesenchymal extracellular matrix.
Tucker
J E
JE
Department of Endodontics, Louisiana State University School of Dentistry, New Orleans 70119.
Lemon
R
R
Mackie
E J
EJ
Tucker
R P
RP
D000818:Animals; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D003238:Connective Tissue / Q000737:chemistry; D003804:Dentin / Q000737:chemistry* / Q000648:ultrastructure; D004285:Dogs; D004848:Epithelium / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005353:Fibronectins / Q000032:analysis*; D005881:Gingiva / Q000737:chemistry*; D007124:Immunoenzyme Techniques; D008297:Male; D013194:Staining and Labeling; D019063:Tenascin
D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D019063:Tenascin