Calcium transport sensitive to ruthenium red in cytochrome oxidase vesicles reconstituted with mitochondrial proteins.
We describe a calcium transport that is sensitive to ruthenium red in liposomes reconstituted with mitochondrial extracts. This system is able to build an internally negative membrane potential, which allows the electrogenic influx of Ca2+ and Sr2+. Proteins with molecular weights higher than 35 kDa were incorporated to the vesicles, and enhanced the accumulation of the cation in an energy-dependent fashion.
Zazueta
C
C
Departamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, México, D.F.
Holguín
J A
JA
Ramírez
J
J
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007700:Kinetics; D008081:Liposomes; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology*
D008081:Liposomes; D012430:Ruthenium Red; D003576:Electron Transport Complex IV; D008274:Magnesium; D002118:Calcium