Change in sensitivity to lysophosphatidylserine of mouse bone marrow-derived mast cells during cultivation with fibroblasts.
Lysophosphatidylserine (lysoPS) is known to enhance IgE-mediated activation of rodent connective tissue mast cells (CTMCs). In the present study, we investigated the effect of lysoPS on degranulation of interleukin-3-dependent mouse bone marrow-derived mucosal mast cells (BMMCs) and of their CTMC-like differentiated cells. In the absence of lysoPS, BMMCs released approximately 20% of their histamine when sensitized with anti-dinitrophenyl (DNP) IgE and challenged with DNP-conjugated antigen. When stimulated in the presence of lysoPS, no appreciable enhancement was observed. On the other hand, histamine release from BMMCs, which had differentiated to CTMC-like cells by co-culture with 3T3 fibroblasts, was enhanced 2- to 3-fold by the addition of lysoPS. The maximum potentiation was observed at 5 x 10(-6) M lysoPS. These results suggest that mast cells might acquire their dependence on exogenous lysoPS during differentiation from mucosal mast cells to CTMC-like cells.
Murakami
M
M
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Umeda
M
M
Kudo
I
I
Inoue
K
K
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D015550:Cell Degranulation / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006636:Histamine Release / Q000187:drug effects*; D007114:Immunization; D008246:Lysophospholipids / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats
D008246:Lysophospholipids; C025059:lysophosphatidylserine