title: The role of alpha 2-macroglobulin in furunculosis: a comparison of rainbow trout and brook trout.
abstract: 1. The molecular basis for the high survival rate of rainbow trout, Oncorhynchus mykiss, infected with furunculosis was investigated. 2. Alpha 2-macroglobulin (alpha 2M), a major serum protease inhibitor, was partially purified from rainbow trout and brook trout, Salvelinus fontinalis, sera; the latter species shows marked disease susceptibility. 3. It is shown that a 10-fold species-based difference in alpha 2M inhibitory activity exists against a furunculosis associated bacterial protease. 4. A possible basis for the observed disparity is discussed. 5. Results suggest that the high mol. wt form of teleost (trout) albumin is a dimer composed of two 85,000 subunits.
authors.affiliation: Department of Fisheries and Wildlife, Utah State University, Logan 84322.
authors.forename: S J
authors.initials: SJ
authors.lastname: Freedman
mesh_terms: D000333:Aeromonas / Q000201:enzymology*; D000818:Animals; D001424:Bacterial Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D005393:Fish Diseases / Q000097:blood / Q000276:immunology; D005667:Furunculosis / Q000097:blood / Q000276:immunology / Q000662:veterinary; D007113:Immunity, Innate; D008970:Molecular Weight; D011480:Protease Inhibitors; D012474:Salmon / Q000097:blood; D012487:Salmonidae / Q000097:blood*; D014337:Trout / Q000097:blood; D000511:alpha-Macroglobulins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D011480:Protease Inhibitors; D000511:alpha-Macroglobulins