Protective anti-sporozoite antibodies induced by a chemically defined synthetic vaccine.
Chemically defined synthetic polymers, known as multiple antigen peptide systems (MAPs) represent an effective and novel approach for engineering peptide-based vaccines. Ten different mono and diepitope MAP models, containing different arrangements and stoichiometry of functional B and/or T helper epitopes from the circumsporozoite protein of Plasmodium berghei were used to immunize mice. High titers of antibody and protective immunity against sporozoite challenge were elicited by MAPs containing T and B epitopes arranged in tandem and in equimolar amounts. These results indicate that MAPs may serve as a basis for developing subunit vaccines to induce high levels of antibodies against sporozoites.
Zavala
F
F
New York University Medical Center, Department of Medical and Molecular Parasitology, NY 10010.
Chai
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology; D000939:Epitopes / Q000138:chemical synthesis; D008288:Malaria / Q000517:prevention & control*; D051379:Mice; D008969:Molecular Sequence Data; D010962:Plasmodium berghei / Q000276:immunology; D016052:Protozoan Vaccines / Q000737:chemistry / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology
D000913:Antibodies, Protozoan; D000939:Epitopes; D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic