Structural chromosomal rearrangements in HpaII-treated human lymphocytes.
Restriction endonucleases have been shown to induce chromosome damage in a variety of cultured cells. We recently reported the coincidence between MspI-induced breakage and the location of common fragile sites. We have extended our study to HpaII, which induced a 4.5-fold increase in total breakage compared to controls. It appeared that a major contribution was given by stable chromosome rearrangements, which were present at a 14-fold increased frequency in comparison to the spontaneous levels. Moreover, several chromosome bands were involved in rearrangements in different cultures from different donors. Notably, HpaII-induced breakage occurred in the same bands where breakpoints of constitutional and neoplastic rearrangements are located.
Tedeschi
B
B
Department of Public Health and Cell Biology, II University of Rome Tor Vergata, Italy.
Porfirio
B
B
Caporossi
D
D
Vernole
P
P
Nicoletti
B
B
D004247:DNA / Q000187:drug effects; D004252:DNA Mutational Analysis; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific / Q000494:pharmacology*; D015321:Gene Rearrangement / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007621:Karyotyping; D008214:Lymphocytes / Q000187:drug effects; D012150:Polymorphism, Restriction Fragment Length
D004247:DNA; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific