Development of sensitive methods for the detection of mycobacteria by DNA probes.
A sensitive method allowing the detection of mycobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.
Rahav
G
G
Department of Clinical Microbiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Sela
S
S
Bercovier
H
H
D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005798:Genes, Bacterial; D005821:Genetic Techniques; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012335:RNA, Ribosomal