Reduction in cab and psb A RNA transcripts in response to supplementary ultraviolet-B radiation.
The cab and psb A RNA transcript levels have been determined in Pisum sativum leaves exposed to supplementary ultraviolet-B radiation. The nuclear-encoded cab transcripts are reduced to low levels after only 4 h of UV-B treatment and are undetectable after 3 days exposure. In contrast, the chloroplast-encoded psb A transcript levels, although reduced, are present for at least 3 days. After short periods of UV-B exposure (4 h or 8 h), followed by recovery under control conditions, cab RNA transcript levels had not recovered after 1 day, but were re-established to ca. 60% of control levels after 2 more days. Increased irradiance during exposure to UV-B reduced the effect upon cab transcripts, although the decrease was still substantial. These results indicate rapid changes in the cellular regulation of gene expression in response to supplementary UV-B and suggest increased UV-B radiation may have profound consequences for future productivity of sensitive crop species.
Jordan
B R
BR
CSIRO, Division of Plant Industry, Canberra ACT, Australia.
Chow
W S
WS
Strid
A
A
Anderson
J M
JM
D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D007887:Fabaceae / Q000235:genetics* / Q000528:radiation effects; D017343:Genes, Plant / Q000528:radiation effects; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D010946:Plants, Medicinal; D012313:RNA / Q000378:metabolism*; D014158:Transcription, Genetic / Q000528:radiation effects*; D014466:Ultraviolet Rays
D002352:Carrier Proteins; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D002734:Chlorophyll; C037184:chlorophyll b; D012313:RNA; D000077194:Chlorophyll A