[Effect of bleomycin A5 with calmodulin inhibitor on the proliferation of S-180 cells in vitro].
The effect of bleomycin A5 (BLM) alone and along with calmodulin inhibitor N-(4-aminobutyl)-5-chloro-2-naphthalene sulfonamide (W-13) on the proliferation of S-180 cells in vitro were studied. IC50 of BLM alone to the cells was about 2.63 micrograms/ml, which was decreased to 1/3.8 and 1/9.5 of 2.63 micrograms/ml when plus W-13 1, 5 micrograms/ml respectively. The results indicated that nontoxic doses of W-13 enhanced the inhibition of cell proliferation under the condition of BLM 0.5-2.5 micrograms/ml. In colony forming test, the survival fraction of S-180 cells treated with BLM plus W-13 was decreased to 1/87-240 of the cells treated with BLM alone. The results suggest that W-13 can enhance antitumor activity of BLM in vitro and may be used as an enhancer of BLM in vivo.
Zhang
H Q
HQ
Department of Biology, Beijing Normal University, China.
He
N G
NG
Xue
S B
SB
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology* / Q000031:analogs & derivatives; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D004357:Drug Synergism; D012510:Sarcoma 180 / Q000473:pathology*; D013449:Sulfonamides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014410:Tumor Stem Cell Assay
D000903:Antibiotics, Antineoplastic; D002147:Calmodulin; D013449:Sulfonamides; D001761:Bleomycin; C025703:bleomycetin; C033772:N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide