title: Structure and localization of the human insulin-like growth factor-binding protein 2 gene.
abstract: Insulin-like growth factor binding proteins (IGFBPs) are extracellular proteins that specifically bind IGF and modulate their effects. The human IGFBP2 gene was studied and shown to be localized to chromosome 2 region q33-q34, by somatic cell hybrid analysis and in situ hybridization. Structural characterization of the gene showed that it consists of four exons with three introns of lengths 27.0, 1.0, and 1.9 kilobase-pairs. Comparison of the encoded protein sequence of each exon in IGFBP1, 2, and 3 reveals the highest amino acid identity, 28%, in exon 1, while the lowest was found in exon 2. However, pairwise sequence comparisons demonstrate 50% identity between the protein sequences encoded by exon 4 in IGFBP1 and 2, while their respective identities with IGFBP3 are only 25 and 30%.
authors.affiliation: Department of Clinical Genetics, Karolinska Institute, Stockholm, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Ehrenborg
authors.forename: S
authors.initials: S
authors.lastname: Vilhelmsdotter
authors.forename: S
authors.initials: S
authors.lastname: Bajalica
authors.forename: C
authors.initials: C
authors.lastname: Larsson
authors.forename: I
authors.initials: I
authors.lastname: Stern
authors.forename: J
authors.initials: J
authors.lastname: Koch
authors.forename: K
authors.initials: K
authors.lastname: Brøndum-Nielsen
authors.forename: H
authors.initials: H
authors.lastname: Luthman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D003360:Cosmids; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D005260:Female; D005796:Genes; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology; D018971:Insulin-Like Growth Factor Binding Protein 2; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010920:Placenta / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D002352:Carrier Proteins; D018971:Insulin-Like Growth Factor Binding Protein 2; D015345:Oligonucleotide Probes; D004247:DNA