N-carboxymethylchitosan-N,O-sulfate as an anti-HIV-1 agent.
N-carboxymethylchitosan-N-O-sulfate (NCMCS), a sulfated polysaccharide derivative of chitin, inhibited the propagation of the human immunodeficiency virus type 1 (HIV-1) in human CD4+ cells and that of Rauscher murine leukemia virus (RLV) in murine fibroblasts. A dose-dependent inhibition of both viruses was observed without significant cytotoxicity. NCMCS blocked the binding of HIV-1 to human CD4+ target cells and competitively inhibited HIV-1 reverse transcriptase. Thus, NCMCS may prevent HIV-1 infection by inhibiting viral adsorption to the CD4 receptor and reverse transcription of the viral genome.
Sosa
M A
MA
Dana-Farber Cancer Institute, Division of Cancer Pharmacology, Boston, Massachusetts 02115.
Fazely
F
F
Koch
J A
JA
Vercellotti
S V
SV
Ruprecht
R M
RM
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D006801:Humans; D007700:Kinetics; D051379:Mice; D011925:Rauscher Virus / Q000187:drug effects* / Q000502:physiology; D018894:Reverse Transcriptase Inhibitors; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D018894:Reverse Transcriptase Inhibitors; D014764:Viral Proteins; C067123:N-carboxymethylchitosan-N-O-sulfate; D002686:Chitin