title: Circulating and localized immune complexes in experimental mycoplasma-induced arthritis-associated ocular inflammation.
abstract: Ocular deposits of immune complexes are believed to contribute to the anterior segment inflammations observed in association with the human arthritides. Arthritis-related ocular inflammations may be reproduced in animals by infection with certain species of mycoplasma. To evaluate the role of immune complexes in the production of ocular lesions, we studied their involvement in the rodent model of experimental arthritis-associated ocular inflammation induced by Mycoplasma arthritidis. Sprague-Dawley rats were infected with viable concentrates of M. arthritidis and monitored for the production of related circulating and intraocular immune complexes. Circulating immune complexes were monitored by antigen capture systems, and localized intraocular complexes were identified by indirect immunohistochemistry. Polyacrylamide gel immunoblot analysis of captured complexes confirmed the antigen(s) involved as proteins derived from M. arthritidis. Indirect immunofluorescence revealed localized complexes containing mycoplasma antigens within the ciliary-iris vasculature. Concentrations of the generated complexes diminished rapidly over a 30-day period. While complex deposits within ocular tissues could represent a contributing cause to the localized anterior segment inflammation reported in this rodent model, secondary challenge with viable M. arthritidis, which reproduced high concentrations of intraocular and circulating immune complexes, failed to elicit any ocular response.
authors.affiliation: University of California, Medical Center, Davis, Sacramento 95816.
authors.forename: C E
authors.initials: CE
authors.lastname: Thirkill
authors.forename: N K
authors.initials: NK
authors.lastname: Tyler
authors.forename: A M
authors.initials: AM
authors.lastname: Roth
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000032:analysis*; D001170:Arthritis, Infectious / Q000276:immunology*; D015153:Blotting, Western; D003231:Conjunctivitis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007150:Immunohistochemistry; D009175:Mycoplasma Infections / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex