The antibody response in BALB/c mice to the Plasmodium falciparum circumsporozoite repetitive epitope covalently coupled to synthetic lipopeptide adjuvant.
The repetitive epitope of Plasmodium falciparum circumsporozoite protein (Asn-Ala-Asn-Pro)3 [(NANP)3] was coupled to tripalmitoyl-S-glyceryl-cysteine (P3C) and tripalmitoyl-S-glyceryl-cysteinyl-serine (P3CS). The lipopeptide P3CS is a potent B-cell and macrophage activator. The resulting immunogenic lipopeptides were used for immunization of the low responder mouse strain BALB/c. These low molecular weight conjugates induced specific anti-(NANP)3 IgG and IgM levels without any carrier proteins or admixed adjuvants after a single administration.
Wiesmüller
K H
KH
Institute of Organic Chemistry, University of Tübingen, Germany.
Jung
G
G
Gillessen
D
D
Loffl
C
C
Bessler
W G
WG
Böltz
T
T
D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008074:Lipoproteins / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008074:Lipoproteins; D009842:Oligopeptides; C052953:(asparaginyl-alanyl-asparaginyl-proline)3