title: Coupling of killer virus transcription with translation in yeast cell-free extracts.
abstract: The cytoplasmically inherited killer virus of Saccharomyces cerevisiae expresses its dsRNA genome via apparently uncapped viral transcripts produced in the cytoplasm of infected cells. Virions of this naturally temperature-sensitive virus can be added to cell-free translational extracts of uninfected yeast cells resulting in a reaction in which viral transcription and translation are coupled at 15 degrees C in vitro. In this reaction nucleotides are incorporated into full-length transcripts of the M and L-A dsRNA segments, with lower levels of incorporation into genomic RNA. In addition, incorporation of nucleotides is observed into a smaller RNA species showing no sequence relatedness to M or L-A.
authors.affiliation: University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Department of Molecular Genetics and Microbiology, Piscataway 08854-5635.
authors.forename: F P
authors.initials: FP
authors.lastname: Barbone
authors.forename: M J
authors.initials: MJ
authors.lastname: Leibowitz
mesh_terms: D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012313:RNA / Q000502:physiology; D015347:RNA Probes / Q000737:chemistry; D012328:RNA Viruses / Q000235:genetics* / Q000502:physiology; D019244:RNA, Satellite; D012367:RNA, Viral / Q000737:chemistry / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013696:Temperature; D014158:Transcription, Genetic; D014771:Virion / Q000235:genetics* / Q000502:physiology
chemical_list: D015347:RNA Probes; D019244:RNA, Satellite; D012367:RNA, Viral; D012313:RNA