Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell lines.
Rat monocyte chemoattractant protein-1 (MCP-1) cDNA was cloned. A cDNA library was constructed from mRNA extracted from Con A-stimulated rat spleen cells. After 2 rounds of screening with a radiolabeled oligonucleotide probe and DNA sequencing from plasmid DNAs, one clone which encoded for MCP-1 was obtained. The cDNA clone comprises 665 base pairs with an open reading frame which encodes for 148 amino acid protein. Spleen cells expressed a significant amount of MCP-1 mRNA without stimulus. But higher expression was observed when cells were stimulated with Con A. MCP-1 mRNA was also expressed in tumor cell lines, although the amount of expressed MCP-1 mRNA was different among cell lines.
Yoshimura
T
T
Immunopathology Section, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702.
Takeya
M
M
Takahashi
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000235:genetics* / Q000494:pharmacology / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003001:Cloning, Molecular; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D051381:Rats; D011916:Rats, Inbred F344; D011994:Recombinant Proteins / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000187:drug effects / Q000378:metabolism*
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D012333:RNA, Messenger; D011994:Recombinant Proteins; D003208:Concanavalin A; D011061:Poly A; D012313:RNA; D004247:DNA