Human milk fat globule membrane glycoproteins express blood group-related determinants primarily on mucin-like epithelial membrane antigens and gp70.
Milk fat globule membranes (MFGM) were prepared from 21 human milks and dot-blotted. MFGM samples were compared with reference to 8 blood group-related antigens reactive with monoclonal antibodies and lectins. All preparations contained epithelial membrane antigen (EMA) and the majority was positive for type 1 Lewis a and b antigens, whereas only trace amounts of sialyl Lewis a were found. For type 2 Lewis antigens, most MFGM reacted intensely for X, but only weakly and infrequently for the Y antigen. Reactivities for H were also infrequent and antigens related to A and/or B (types 1 and 2) were not found. Western blot analyses established that these antigenic determinants were borne mainly by mucin-like components and gp70.
Dion
A S
AS
Center for Molecular Medicine & Immunology, Newark, NJ 07103.
Williams
C J
CJ
Herlyn
M
M
Major
P
P
D000017:ABO Blood-Group System / Q000276:immunology; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001789:Blood Group Antigens / Q000276:immunology*; D015153:Blotting, Western; D000939:Epitopes / Q000276:immunology; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*; D008892:Milk / Q000276:immunology; D008970:Molecular Weight; D018396:Mucin-1; D009077:Mucins / Q000276:immunology*
D000017:ABO Blood-Group System; D000941:Antigens; D001789:Blood Group Antigens; D000939:Epitopes; D006023:Glycoproteins; D007983:Lewis Blood Group Antigens; D008562:Membrane Glycoproteins; D018396:Mucin-1; D009077:Mucins