title: Metabolic and structural effects of HIV infection in human peripheral blood mononuclear cells can be monitored with 1H NMR spectroscopy.
abstract: Infection of human peripheral blood lymphocytes by human immunodeficiency virus type 1 (HIV-1) was investigated by means of 1H nuclear magnetic resonance spectroscopy, taking advantage of the presence of signals from fluid lipid domains in the membrane of stimulated lymphocytes. A transient decrease of the lipid methylene signal intensity was observed at the time of HIV internalization, monitoring a general rearrangement of membrane structure associated with virus entry. A similar effect was also observed a few days after infection, when HIV particles are released by infected cells as demonstrated by high reverse transcriptase activity in cell supernatant. Signals arising from choline-based metabolites were also affected by HIV infection, indicating a possible slowing down of phospholipid synthesis.
authors.affiliation: Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Federico
authors.forename: M T
authors.initials: MT
authors.lastname: Maggiorella
authors.forename: N
authors.initials: N
authors.lastname: Sulli
authors.forename: L
authors.initials: L
authors.lastname: Guidoni
authors.forename: A M
authors.initials: AM
authors.lastname: Luciani
authors.forename: A
authors.initials: A
authors.lastname: Rosi
authors.forename: V
authors.initials: V
authors.lastname: Viti
authors.forename: G B
authors.initials: GB
authors.lastname: Rossi
authors.forename: P
authors.initials: P
authors.lastname: Verani
mesh_terms: D002474:Cell-Free System; D002478:Cells, Cultured; D015658:HIV Infections / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D054303:HIV Reverse Transcriptase; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008213:Lymphocyte Activation; D009682:Magnetic Resonance Spectroscopy; D015166:Monitoring, Immunologic; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D013997:Time Factors
chemical_list: D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase