[A new pan-macrophage antibody Ki-M1P stains plasmacytoid cells in paraffin sections of lymph nodes].
Plasmacytoid, T cells (PTC) occurring in cases of chronic non-specific lymphadenitis were investigated using a panel of monoclonal antibodies and cryostat sections. From the typical T cell antigens only CD4 was detectable on PTC. Antibodies directed against myelo-monocytic antigens such as Ki-M2, Ki-M6 (CD68), and Ki-M7 revealed positive reaction with these cells. The recently established monoclonal antibody Ki-M1P reactive with monocyte/macrophages shows a surface and granular cytoplasmic reactivity with PTC. This observation may indicate the myelo-monocytic origin. Ki-M1P detects a formalin resistant antigen and is thus applicable to conventionally processed paraffin sections as demonstrated in twenty cases of hystiocytic necrotizing lymphadenitis (KIKUCHI).
Wacker
H H
HH
Institut für Pathologie, Universität Kiel.
Hansmann
M L
ML
Lumbeck
H
H
Radzun
H J
HJ
Parwaresch
M R
MR
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology* / Q000473:pathology; D008199:Lymphadenitis / Q000276:immunology* / Q000473:pathology; D008264:Macrophages / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic
Ein neuer Pan-Makrophagen Antikörper Ki-M1P erkennt plasmozytoide Lymphknotenzellen in Paraffinschnitten.