Immunological relevance of malonic dialdehyde (MDA): IV. Further evidences about the epitope recognized by antibodies obtained from rabbits immunized with MDA-modified lysozyme.
Reactions of MDA with primary amino groups produce inter- or intra-molecular 1-amino-3-imino-propene (AIP) bridges, leading to structural modifications of biological molecules. In this work, applying electrophoresis followed by transfer onto nitrocellulose membranes, we observed that serum of a rabbit immunized with MDA-modified lysozyme (ML) reacts not only with ML and native lysozyme (L), but also with MDA-modified ribonuclease, cytochrome c or polylysine (MR, MC and MP respectively), while it does not react with native ribonuclease, cytochrome c or polylysine (R, C and P respectively). These results confirm previous ones indicating that sera of rabbits immunized with ML contain antibodies reacting specifically with epitopes containing AIP bridges.
Chancerelle
Y
Y
Centre de Recherches du Service de Santé des Armées, La Tronche, France.
Alban
C
C
Viret
R
R
Tosetti
F
F
Kergonou
J F
JF
D000818:Animals; D000906:Antibodies; D000939:Epitopes; D007114:Immunization; D007120:Immunochemistry; D008315:Malondialdehyde / Q000276:immunology*; D009113:Muramidase / Q000276:immunology; D011817:Rabbits
D000906:Antibodies; D000939:Epitopes; D008315:Malondialdehyde; D009113:Muramidase