Higher order structure of the ribosomal 5 S RNA.
The structure of the ribosomal 5 S RNA was examined using Fe(II)-EDTA, a solvent-based reagent that cleaves the phosphodiester backbone of both double- and single-stranded RNA but is restricted by the three-dimensional structure. In the yeast 5 S RNA, cleavages were significantly restricted in six specific regions of the molecule; restrictions in only two of these regions were clearly dependent on a high salt/magnesium ion environment. A comparison of four RNAs of diverse origin revealed strong similarities in the cleavage profiles supporting a highly conserved higher order structure. Taken together with previous studies these data provide a more detailed modeling of the three-dimensional structure.
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Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder 80309-0215.
D000818:Animals; D001483:Base Sequence; D004492:Edetic Acid / Q000737:chemistry; D004926:Escherichia coli / Q000648:ultrastructure; D005296:Ferrous Compounds / Q000737:chemistry; D006217:Halobacterium / Q000648:ultrastructure; D066298:In Vitro Techniques; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000648:ultrastructure; D012331:RNA, Fungal / Q000737:chemistry / Q000648:ultrastructure; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000648:ultrastructure*; D051381:Rats; D012441:Saccharomyces cerevisiae
D005296:Ferrous Compounds; D012329:RNA, Bacterial; D012331:RNA, Fungal; D012341:RNA, Ribosomal, 5S; D004492:Edetic Acid