title: Role of sn-1-saturated,sn-2-polyunsaturated phospholipids in control of membrane receptor conformational equilibrium: effects of cholesterol and acyl chain unsaturation on the metarhodopsin I in equilibrium with metarhodopsin II equilibrium.
abstract: The effect of phospholipid bilayer acyl chain packing free volume on the equilibrium concentration of the form of photolyzed rhodopsin which initiates visual signal transduction, metarhodopsin II (meta II), is examined in reconstituted systems formed from the saturated phospholipid dimyristoylphosphatidylcholine (DMPC) and in the polyunsaturated phospholipid sn-1-palmitoyl-sn-2-arachidonoylphosphatidylcholine (PAPC) with and without 30 mol% cholesterol. The extent of meta II formation is determined from both flash photolysis measurements and rapidly acquired absorbance spectra. Equilibrium and dynamic properties of the lipid bilayer are characterized by the dynamic fluorescence properties of 1,6-diphenyl-1,3,5-hexatriene (DPH). DPH orientational properties are characterized by fv, a parameter which reflects the volume available for probe reorientation in the bilayer, relative to that available in an unhindered, isotropic environment [Straume, M., & Litman, B. J. (1987) Biochemistry 26, 5121-5126]. The metarhodopsin I in equilibrium with meta II equilibrium constant, Keq has a linear relationship with fv for rhodopsin in PAPC vesicles with and without cholesterol as well as for rhodopsin in DMPC vesicles, and these two correlation lines have different slopes. The correlations between Keq and fv in PAPC and DMPC systems are compared with a similar correlation in the native rod outer segment disk membrane and one reported previously in an egg phosphatidylcholine (egg PC) system [Mitchell, D. C., Straume, M., Miller, J. L., & Litman, B. J. (1990) Biochemistry 29, 9143-9149].(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biochemistry, University of Virginia Health Sciences Center, Charlottesville 22908.
authors.forename: D C
authors.initials: DC
authors.lastname: Mitchell
authors.forename: M
authors.initials: M
authors.lastname: Straume
authors.forename: B J
authors.initials: BJ
authors.lastname: Litman
mesh_terms: D000818:Animals; D002417:Cattle; D004134:Dimyristoylphosphatidylcholine / Q000494:pharmacology*; D007700:Kinetics; D008051:Lipid Bilayers; D010742:Phospholipid Ethers / Q000494:pharmacology*; D011487:Protein Conformation; D012243:Rhodopsin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000378:metabolism; D013053:Spectrophotometry
chemical_list: D008051:Lipid Bilayers; D010742:Phospholipid Ethers; C013110:metarhodopsins; C043051:1-O-hexadecyl-2-arachidonyl-sn-glycero-3-phosphocholine; D012243:Rhodopsin; D004134:Dimyristoylphosphatidylcholine