Characterization of T- and B-cell epitopes of a simian retrovirus (SRV-2) envelope protein.
Synthetic envelope peptides of a simian retrovirus (SRV-2) were used to define both T- and B-cell epitopes of the envelope protein. The SRV-2 peptide 100-106 specifically blocks rhesus anti-SRV-2 neutralizing antibody activity, and a peptide 100-106 keyhole limpet hemocyanin conjugate induces a strong antipeptide antibody response. SRV-2 peptide 100-106 and 233-249 induces good T-cell proliferation of murine spleen cells immunized with the SRV-2 virus. Thus, SRV-2 envelope peptide 100-106 represents both a T- and B-cell epitope, and peptide 233-249 a T-cell epitope.
Malley
A
A
Oregon Regional Primate Research Center, Beaverton 97006.
Werner
L
L
Benjamini
E
E
Leung
C Y
CY
Torres
J
J
Pangares
N
N
Shiigi
S
S
Axthelm
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D012191:Retroviridae Proteins / Q000737:chemistry / Q000276:immunology*; D015301:Retroviruses, Simian / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
D000914:Antibodies, Viral; D000939:Epitopes; D007106:Immune Sera; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins