Immunoscintigraphy of experimental transplantable tumours using monoclonal antibody against myelin basic protein.
Monoclonal antibody was prepared against myelin basic protein a so-called pancarcinoma antigen. After labelling with 131I the monoclonal antibody was injected into Lewis-lung cancer mice and rats with Walker breast cancer. Two, 24, 48, 72 and 96 hours after the labelled monoclonal antibody injection, radioimmunoimaging studies were carried out. After each gamma-camera study, organ distribution of the labelled monoclonal antibody was determined with radiobioassay technique which showed significantly higher activity in the tumour tissue than in healthy ones. Significant sample radioactivity could be recovered in the tumour masses 48 hours after injection, which persisted even after 96 hours. The later finding might enable diagnosing types of malignancy with isotope-labelled monoclonal antibody against myelin basic protein.
Kövesi
G
G
Department of Maxillofacial Surgery, Semmelweis University of Medicine, Budapest, Hungary.
Mohari
K
K
Kocsár
L
L
Fekete
B
B
Szilvási
I
I
Szabó
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002277:Carcinoma / Q000000981:diagnostic imaging* / Q000276:immunology; D007457:Iodine Radioisotopes; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D004676:Myelin Basic Protein / Q000276:immunology*; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D004676:Myelin Basic Protein