Involvement of OmpF during reception and translocation steps of colicin N entry.
[125I]-colicin N binds to OmpF receptor sites (70,000 per cell) with an average Kassoc of 3.2 x 10(6) M-1 at 23 degrees C. Monoclonal antibody directed against a cell-surface-exposed epitope of OmpF is able to complete with the binding of the colicin in vitro and also to protect against colicin N in vivo. OmpF is an absolute requirement for colicin N uptake. OmpC cannot serve as a substitute for OmpF during translocation across the outer membrane under receptor bypass conditions, which is in contrast to colicin A. Colicin N does not cross-react with various monoclonal antibodies directed against colicin A.
Bourdineaud
J P
JP
Centre de Biochimie et de Biologie Moléculaire, CNRS, Marseille, France.
Fierobe
H P
HP
Lazdunski
C
C
Pagès
J M
JM
D000911:Antibodies, Monoclonal / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D001667:Binding, Competitive; D015153:Blotting, Western; D003087:Colicins / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D004926:Escherichia coli / Q000378:metabolism; D010957:Plasmids
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D003087:Colicins; D000939:Epitopes