Specific, high-affinity binding sites for angiotensin II on Mycoplasma hyorhinis.
Mycoplasmataceae are known to express various proteins that are similar to those present in mammals. We report a strain of Mycoplasma hyorhinis isolated from opossum kidney cells with specific, high-affinity binding sites for human angiotensin II (Kd = 5.1 +/- 1.9 nM). In contrast, two strains of M. hominis revealed no specific binding. These binding sites resembled mammalian angiotensin II receptors by their high affinity and by their sensitivity to dithiothreitol. However, they are different from mammalian angiotensin II receptors in that they bind angiotensin I with high affinity (Kd = 1.6 +/- 0.29 nM) but not angiotensin III (Kd approximately 330,000 nM). [125I]-angiotensin II binding was not inhibited by angiotensin receptor subtype antagonists DuP 753 and CGP 42112A but it was sensitive to bacitracin and aprotinin. Positions Asp1, Ile5, His6 and Pro7 were essential for binding to M. hyorhinis as deletion of these residues led to a more than 10,000-fold decrease in affinity.
Bergwitz
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Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Boston 02114.
Madoff
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Abou-Samra
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Jüppner
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D000803:Angiotensin I / Q000378:metabolism*; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D001414:Bacitracin / Q000494:pharmacology; D001667:Binding, Competitive; D002460:Cell Line; D004229:Dithiothreitol / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D009174:Mycoplasma / Q000378:metabolism*; D011869:Radioligand Assay; D011945:Receptors, Angiotensin / Q000187:drug effects / Q000378:metabolism*; D013045:Species Specificity
D011945:Receptors, Angiotensin; D000804:Angiotensin II; D001414:Bacitracin; D000803:Angiotensin I; D007611:Aprotinin; D004229:Dithiothreitol