Cell death in the human leukemia cell line, K-562, induced by antiserum and monoclonal antibodies.
Rabbit polyclonal antiserum, or derived gamma globulin, to K-562 cells induces decreased TdR uptake within hours and cell death without cytolysis in 2-4 days. A panel of nine mAb, reactive with K-562 cells, was grouped on the basis of no effect on growth or TdR uptake, increased uptake, or decreased uptake. Treatment of cells with antiserum, gamma globulin, or mAb of the last group caused single-strand, but not double-strand, DNA fragmentation at a time when the cells were still viable. Cycloheximide did not inhibit the antibody effect suggesting that protein synthesis was not required. Aurintricarboxylic acid at certain concentrations markedly enhanced TdR uptake and protected the cells when antiserum was used but did not protect from mAb treatment.
Wust
C J
CJ
Department of Microbiology, University of Tennessee, Knoxville 37996.
Hodge
J W
JW
Ichiki
A T
AT
Lozzio
C B
CB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D002470:Cell Survival; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000187:drug effects; D004277:DNA, Single-Stranded / Q000187:drug effects; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000378:metabolism* / Q000473:pathology; D007951:Leukemia, Myeloid / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis; D011817:Rabbits; D014407:Tumor Cells, Cultured; D005719:gamma-Globulins / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D007106:Immune Sera; D009363:Neoplasm Proteins; D005719:gamma-Globulins; D001312:Aurintricarboxylic Acid