Serotonin regulation of tachykinin biosynthesis in the rat neostriatum.
Serotonin (5-HT) neurotransmission was altered to determine its role in regulating the biosynthesis of tachykinins in the neostriatum (NS). Depletion of 5-HT with subchronic p-chlorophenylalanine (pCPA) treatment decreased preprotachykinin (PPT, the prohormone precursor to SP) mRNA levels in the NS. By contrast, raising extracellular 5-HT levels with zimelidine (a 5-HT uptake inhibitor) or clorgyline (a monoamine oxidase inhibitor) resulted in increased levels of PPT mRNA. To determine whether 5-HT receptors played a role in mediating the changes in PPT mRNA, animals were treated with the 5-HT2 agonist DOI. This drug significantly increased both PPT mRNA and SP-like immunoreactivity in the NS. These results together indicate that neostriatal tachykinin biosynthesis is sensitive to alterations in 5-HT neurotransmission.
Walker
P D
PD
Department of Biological Sciences, Rutgers University, Newark, NJ 07102.
Riley
L A
LA
Hart
R P
RP
Jonakait
G M
GM
D000818:Animals; D015152:Blotting, Northern; D002421:Caudate Nucleus / Q000502:physiology*; D010134:Fenclonine / Q000494:pharmacology; D008995:Monoamine Oxidase / Q000378:metabolism; D011498:Protein Precursors / Q000096:biosynthesis; D011699:Putamen / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D011985:Receptors, Serotonin / Q000187:drug effects; D012701:Serotonin / Q000502:physiology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D013373:Substance P / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D015320:Tachykinins / Q000096:biosynthesis*; D014365:Tryptophan Hydroxylase / Q000378:metabolism
D011498:Protein Precursors; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D015320:Tachykinins; C044206:preprotachykinin; D012701:Serotonin; D013373:Substance P; D014365:Tryptophan Hydroxylase; D008995:Monoamine Oxidase; D010134:Fenclonine