Isoelectric focusing electrophoresis of lignin.
Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.
Niku-Paavola
M L
ML
VTT, Biotechnical Laboratory, Espoo, Finland.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods*; D008031:Lignin / Q000302:isolation & purification*; D010544:Peroxidases; D012834:Silver; D013055:Spectrophotometry, Infrared; D013194:Staining and Labeling; D014466:Ultraviolet Rays
D012834:Silver; D008031:Lignin; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase