[A stable reagent for the-single stage determination of inorganic phosphate].
A recipe of a simple reagent for phosphorus detection has been developed, consisting of ammonium molybdate (4 mM), sulfuric acid (0.2 N), and Tween-80 (0.2%). The developing phosphate staining may be registered in 15 min at a wavelength of 350 nm. The product molar extinction is equal to 1.20.10(4) M-1.cm-1, this being close to that of molybdic blue. Phosphate staining is characterized by the stability of results and insensitivity to the presence of a number of substances used in enzymology. The prepared reagent is fit for experiments within a fortnight if stored in the cold.
Pupyshev
A B
AB
D007202:Indicators and Reagents; D010758:Phosphorus / Q000032:analysis*; D013194:Staining and Labeling
D007202:Indicators and Reagents; D010758:Phosphorus
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