title: The stringent response in Streptomyces coelicolor A3(2).
abstract: The stringent response was elicited in the antibiotic producer Streptomyces coelicolor A3(2) either by amino acid depletion (nutritional shiftdown) or by the addition of serine hydroxamate; both led to increased levels of ppGpp and to a reduction in transcription from the four promoters of the rrnD rRNA gene set. Analysis of untreated batch cultures revealed elevated ppGpp levels at the end of exponential growth, preceding the onset of antibiotic production. The effect of provoking the stringent response on antibiotic production in exponentially growing cultures was assessed by S1 nuclease mapping of actIII, an early gene of the actinorhodin biosynthetic cluster. Expression of actIII occurred after nutritional shiftdown, but not after treatment with serine hydroxamate. Although the need for ppGpp in triggering antibiotic production remains equivocal, ppGpp synthesis alone does not appear to be sufficient to initiate secondary metabolism in S. coelicolor A3(2).
authors.affiliation: John Innes Institute, John Innes Centre for Plant Science Research, Norwich, UK.
authors.forename: E
authors.initials: E
authors.lastname: Strauch
authors.forename: E
authors.initials: E
authors.lastname: Takano
authors.forename: H A
authors.initials: HA
authors.lastname: Baylis
authors.forename: M J
authors.initials: MJ
authors.lastname: Bibb
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000880:Anthraquinones; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D005798:Genes, Bacterial; D006159:Guanosine Tetraphosphate / Q000378:metabolism*; D007700:Kinetics; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis / Q000235:genetics; D012694:Serine / Q000031:analogs & derivatives / Q000494:pharmacology; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D000095622:Benzoisochromanequinones
chemical_list: D000596:Amino Acids; D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D006159:Guanosine Tetraphosphate; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012694:Serine; D015719:Single-Strand Specific DNA and RNA Endonucleases; C028002:serine hydroxamate; C013390:actinorhodin; D000095622:Benzoisochromanequinones