The stimuli releasing histamine from murine bone marrow-derived mast cells. 2. Mechanisms involved in histamine release induced by extracellular ATP and its metabolites.
Extracellular ATP stimulated histamine release and generation of leukotrience C4 (LTC4) accompanied with the formation of inositol phosphates and a rapid increase in intracellular Ca2+ ([Ca2+]i) in mouse bone marrow-derived cultured mast cells (BMMC). The rank order of histamine-releasing potency of ATP and its metabolites is ATP greater than ADP greater than AMP greater than adenosine. Nonhydrolyzable ATP analog, adenosine-5'-O-[2-thiotriphosphate] (ATP-S) released more histamine from the cells than ATP. On the other hand, simultaneous addition of adenosine analogues at micromolar concentrations potentiated histamine release from the cells induced by ATP (50 microM) or DNP-HSA antigen (0.1 ng/ml) in the following rank order: adenosine greater than AMP much greater than ADP = ATP. Histamine release potentiated by adenosine was blocked by the treatment with pertussis toxin, whereas histamine release induced by ATP was not affected by the toxin, suggesting that extracellular ATP stimulate histamine release from BMMC probably via mechanisms independent of the potentiation of histamine release induced by adenosine.
Saito
H
H
Division of Allergy, National Children's Medical Research Center.
Sakaguchi
N
N
Ebisawa
M
M
Matsumoto
K
K
Akasawa
A
A
Iikura
Y
Y
D000241:Adenosine / Q000276:immunology; D000244:Adenosine Diphosphate / Q000276:immunology; D000249:Adenosine Monophosphate / Q000276:immunology; D000255:Adenosine Triphosphate / Q000276:immunology*; D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D006636:Histamine Release; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism*; D051379:Mice
D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000241:Adenosine