Status of ornithine decarboxylase activity and DNA synthesis in mancozeb-exposed mouse skin.
The effect of mancozeb, a fungicide, on mouse skin ornithine decarboxylase (ODC) activity and DNA synthesis was studied. ODC activity was induced after topical application of mancozeb and exhibited a peak level at 5 h. This ODC induction was dependent on the dose of mancozeb applied. Cycloheximide, an inhibitor of protein synthesis, inhibited the mancozeb-caused ODC induction, indicating the effect on enzyme protein synthesis. The rate of DNA synthesis was also increased by mancozeb, as indicated by increased [3H]thymidine incorporation into skin DNA. Induction of ODC activity and DNA synthesis are among the events probably involved in the tumorigenic action of mancozeb on mouse skin.
Gupta
K P
KP
Laboratory of Environmental Carcinogenesis, Industrial Toxicology Research Centre, Lucknow, India.
Mehrotra
N K
NK
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D005659:Fungicides, Industrial / Q000633:toxicity*; D008344:Maneb / Q000633:toxicity*; D051379:Mice; D009955:Ornithine Decarboxylase / Q000032:analysis*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012878:Skin Neoplasms / Q000139:chemically induced; D013755:Tetradecanoylphorbol Acetate; D015038:Zineb / Q000633:toxicity*
D005659:Fungicides, Industrial; D008344:Maneb; D004247:DNA; D003513:Cycloheximide; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate; C013099:mancozeb; D015038:Zineb