The tetrazolium-formazan system: design and histochemistry.
Our increasing knowledge about the chemistry and the correlations between chemical structure and histochemical properties of the tetrazolium/formazan system is resulting in: a better understanding of existing histochemical tetrazolium techniques; the selection of optimal tetrazolium salts for qualified use in histochemistry, cytochemistry and biochemistry; both qualitative and quantitative improvements in histochemical techniques for purposes demonstrating the activities of various dehydrogenating enzymes; an extended insight into the "state" of the tested biological object by means of tetrazolium indicators with special properties; and the combination of histochemical enzyme determination with further morphological techniques. This article has attempted to illustrate the progress in the use of the tetrazolium/formazan-system for histochemical purposes.
Seidler
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Institute of Anatomy, Humboldt University Berlin, FRG.
D005562:Formazans / Q000737:chemistry*; D006651:Histocytochemistry / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods; D009993:Osmium Tetroxide / Q000737:chemistry; D013194:Staining and Labeling; D013778:Tetrazolium Salts / Q000737:chemistry*
D005562:Formazans; D013778:Tetrazolium Salts; D009993:Osmium Tetroxide