title: Catabolite inactivation, cyclic AMP and protein phosphorylation in the methylotrophic yeast Hansenula polymorpha.
abstract: The inactivation of the peroxisomal enzyme alcohol oxidase and the cytoplasmic enzymes fructose-1,6-bisphosphatase, malate dehydrogenase and phosphoenolpyruvate carboxykinase was found to occur after addition of glucose to methanol-grown cells of the yeast Hansenula polymorpha. The concentration of cyclic AMP increased nearly twofold within 3 min under the same conditions. In crude extracts of H. polymorpha about 20 proteins are phosphorylated by cyclic AMP dependent protein kinases, among them also fructose-1,6-bisphosphatase. No phosphorylation of the alcohol oxidase protein could be detected. From this fact, it was concluded that the inactivation of the peroxisomal alcohol oxidase is independent of cyclic AMP-dependent protein phosphorylation.
authors.affiliation: Institut für Angewandte und Technische Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, Germany.
authors.forename: K H
authors.initials: KH
authors.lastname: Hofmann
authors.forename: E
authors.initials: E
authors.lastname: Polnisch
mesh_terms: D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D009249:NADP / Q000378:metabolism*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism*; D010766:Phosphorylation; D010843:Pichia / Q000201:enzymology / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D005656:Fungal Proteins; D009249:NADP; D000242:Cyclic AMP; D000429:Alcohol Oxidoreductases; D008291:Malate Dehydrogenase; C019643:alcohol oxidase; D011494:Protein Kinases; D006597:Fructose-Bisphosphatase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D000432:Methanol