title: Two vaccinia virus recombinants expressing HBsAg with different concentration of A- and pre-S2 antigenic determinants.
abstract: Comparative studies of two vaccinia virus (VV) recombinants expressing the hepatitis B virus (HBV) surface antigen (HBsAg) including the pre-S2 region (M-protein) showed that the L-pre-S2/15 recombinant expressed 5-fold more HBsAg as determined by the content of a-determinant than the recombinant v137. However, both recombinants expressed comparable amounts of the pre-S2 antigenic determinant as assessed by enzyme immunoassay with monoclonal antibodies. According to our calculations, one HBsAg unit expressed by the recombinant v137 contained 7-9 times more pre-S2 antigen than did one HBsAg unit expressed by the L-pre-S2/15 recombinant. Binding of pre-S2 region to polymerized human serum albumin was shown not to be an efficient assay at low pre-S2 concentration. HBsAg expressed by the v137 recombinant was less extensively secreted from cells as compared to that expressed by L-pre-S2/15 recombinant. Both recombinants induced the production of antibodies to the pre-S2 antigenic determinant in rabbits. L-pre-S2/15 induced anti-HBsAg a-determinant antibody as well.
authors.affiliation: Institute for Viral Preparations of the U.S.S.R. AMS, Moscow.
authors.forename: N V
authors.initials: NV
authors.lastname: Chelyapov
authors.forename: L E
authors.initials: LE
authors.lastname: Rakhilina
authors.forename: T P
authors.initials: TP
authors.lastname: Antonova
authors.forename: V I
authors.initials: VI
authors.lastname: Chernos
authors.forename: V G
authors.initials: VG
authors.lastname: Grigorjev
authors.forename: E A
authors.initials: EA
authors.lastname: Chudakova
authors.forename: G V
authors.initials: GV
authors.lastname: Pashvykina
authors.forename: L G
authors.initials: LG
authors.lastname: Zakharova
authors.forename: A D
authors.initials: AD
authors.lastname: Altstein
authors.forename: L
authors.initials: L
authors.lastname: Kutinová
mesh_terms: D000818:Animals; D000939:Epitopes / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D011498:Protein Precursors / Q000032:analysis / Q000276:immunology*; D011817:Rabbits; D011995:Recombination, Genetic / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology*
chemical_list: D000939:Epitopes; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050807:presurface protein 2, hepatitis B surface antigen