A C-nucleotide base pair: methylpseudouridine-directed incorporation of formycin triphosphate into RNA catalyzed by T7 RNA polymerase.
With templates containing 2'-deoxy-1-methylpseudouridine (dm psi), T7 RNA polymerase catalyzes the incorporation of either adenosine triphosphate (ATP) or formycin triphosphate (FTP) into a growing chain of RNA with the same efficiency as with templates containing thymidine (dT). In each case, the overall rate of synthesis of full-length products containing formycin is about one-tenth of the rate of synthesis of analogous products containing adenosine. Analysis of the products of abortive initiation shows that incorporation of FMP into the growing oligonucleotide by T7 RNA polymerase is more likely to lead to premature termination of transcription than is incorporation of AMP. Nevertheless, the results demonstrate that T7 RNA polymerase tolerates the formation of a C-nucleotide transcription complex in which the nucleoside bases on both the template and the incoming nucleotide are joined to the ribose by a carbon-carbon bond. This result increases the prospects for further expanding the genetic alphabet via incorporation of new base pairs with novel hydrogen-bonding schemes (Piccirilli et al., 1990).
Piccirilli
J A
JA
Laboratory for Organic Chemistry, Swiss Federal Institute of Technology, Zurich.
Moroney
S E
SE
Benner
S A
SA
D000255:Adenosine Triphosphate / Q000737:chemistry; D001482:Base Composition; D001483:Base Sequence; D002384:Catalysis; D004247:DNA / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry*; D005573:Formycins / Q000737:chemistry*; D008969:Molecular Sequence Data; D011560:Pseudouridine / Q000031:analogs & derivatives* / Q000494:pharmacology; D012313:RNA / Q000737:chemistry*; D012265:Ribonucleotides / Q000737:chemistry*; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014764:Viral Proteins
D005573:Formycins; D012265:Ribonucleotides; D014764:Viral Proteins; D011560:Pseudouridine; C032552:formycin triphosphate; D012313:RNA; C034685:2'-deoxy-3-methylpseudouridine; D000255:Adenosine Triphosphate; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases