title: Simultaneous determination of isbufylline and its major metabolites in rabbit blood and urine by reversed-phase high-performance liquid chromatography.
abstract: A sensitive high-performance liquid chromatographic assay for isbufylline and its major metabolites in rabbit blood and urine is described. After extraction, samples were eluted by a linear reversed-phase gradient. Specimens obtained after intravenous administration of isbufylline to rabbits were analysed to identify and subsequently quantify the potential metabolites. Using the ultraviolet absorption trace on the recorder as a reference, elution fractions were collected and analysed by mass spectrometry with the direct inlet system and gas chromatography-mass spectrometry after derivatization. Seven metabolites were identified and another five quantified. The method is specific, accurate, reproducible and recommended for pharmacokinetic studies.
authors.affiliation: Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, S. Maria Imbaro, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Celardo
authors.forename: G
authors.initials: G
authors.lastname: Dell'Elba
authors.forename: R
authors.initials: R
authors.lastname: Frassanito
authors.forename: E
authors.initials: E
authors.lastname: Benfenati
authors.forename: M
authors.initials: M
authors.lastname: Bonati
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000652:urine; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D010276:Parasympatholytics / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010276:Parasympatholytics; C058878:isbufylline; D015056:1-Methyl-3-isobutylxanthine