Quantitation of DNA and RNA in crude tissue extracts by flow injection analysis.
An automated two-dye flow injection analysis system to quantitate DNA and RNA in crude extracts of tissues is described. The method uses the fluorochrome dyes ethidium bromide and Hoechst 33258. DNA concentration is determined directly from its fluorescence in Hoechst dye. RNA is estimated from fluorescence in ethidium bromide after subtraction of the fluorescence due to DNA. This method has several advantages: a simple extraction procedure, a low detection limit (0.01 micrograms DNA and 0.10 micrograms RNA), automation, and a high sample throughput.
Caldarone
E M
EM
National Oceanic and Atmospheric Administration, Narragansett Laboratory, Rhode Island 02882-1199.
Buckley
L J
LJ
D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D006690:Bisbenzimidazole; D002417:Cattle; D004247:DNA / Q000032:analysis*; D004996:Ethidium; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry; D008297:Male; D009132:Muscles / Q000737:chemistry; D012313:RNA / Q000032:analysis*; D012331:RNA, Fungal / Q000032:analysis; D012441:Saccharomyces cerevisiae; D012474:Salmon; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013094:Spermatozoa / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry
D007202:Indicators and Reagents; D012331:RNA, Fungal; D012313:RNA; D004247:DNA; D004996:Ethidium; D006690:Bisbenzimidazole