Structure, expression and in vitro functional characterization of a novel RNA binding zinc finger protein from Xenopus.
Large multigene families of zinc finger proteins are expressed in vertebrates. One way of approaching their function is to characterize their structure, expression and biochemical properties. XFG 5-1 is a Xenopus zinc finger protein which is widely transcribed in oocytes, embryos and adult tissues. It carries a novel, non-finger repeat structure, which is common to a subfamily of Xenopus zinc finger proteins. The bacterially expressed protein exhibits specific RNA homopolymer binding activities with the zinc finger domain being sufficient for this ability. These findings suggest that XFG 5-1 serves a general biological function involving its RNA binding capacity.
Köster
M
M
Abteilung Biochemie, Universität Ulm, FRG.
Kühn
U
U
Bouwmeester
T
T
Nietfeld
W
W
el-Baradi
T
T
Knöchel
W
W
Pieler
T
T
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D014981:Xenopus / Q000378:metabolism*; D029867:Xenopus Proteins; D016335:Zinc Fingers / Q000235:genetics
D016601:RNA-Binding Proteins; D029867:Xenopus Proteins; C071008:XFG 5-1 protein, Xenopus; D012313:RNA; D004247:DNA