Identification of a mouse cDNA fragment whose expressed polypeptide reacts with anti-recA antibodies.
We have previously reported the in vivo detection of a mouse nuclear protein that cross-reacts with antibodies raised against E coli recA protein. Here, we characterize monospecific anti-recA antibodies, their use for the immunological screening of a cDNA expression library and the isolation of a mouse cDNA fragment which codes for a polypeptide recognized by anti-recA antibodies. The cDNA fragment is 601 nucleotide long and was called KIN17(601). It contains an open reading frame coding for a 200 amino acid polypeptide. In kin17(200) polypeptide, there are amino acids identical to those that form one of the major antigenic determinants of recA protein. Kin17(200) polypeptide also displays a significant similarity with the helix 1 motif of several homeoproteins.
Angulo
J F
JF
Groupe d'Etude Mutagenèse et Cancérogenèse, CNRS, Gif-sur-Yvette, France.
Rouer
E
E
Benarous
R
R
Devoret
R
R
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000915:Antibody Affinity; D000918:Antibody Specificity; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D000939:Epitopes / Q000276:immunology; D005801:Genes, Homeobox; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000276:immunology; D011938:Rec A Recombinases / Q000235:genetics / Q000276:immunology*; D012689:Sequence Homology, Nucleic Acid
D000907:Antibodies, Bacterial; D000939:Epitopes; D010455:Peptides; D004247:DNA; D011938:Rec A Recombinases