title: Construction, purification and characterization of a recombinant baculovirus containing the gene for alpha subunit of human chorionic gonadotropin.
abstract: A cDNA encoding the alpha subunit of human chorionic gonadotropin, a placental glycoprotein hormone, was cloned downstream to the viral polyhedrin gene promoter of Autographa california nuclear polyhedrosis virus and the recombinant transfer vector was used to co-transfect Spodoptera frugiperda cells growing in culture. Recombinant baculovirus carrying the alpha hCG gene was detected and isolated after dot hybridization using supernatant from co-transfected cells. Recombinant vAc alpha hCG having a replacement of the viral polyhedrin gene, which is hyper-transcribed very late in the infection cycle, with the alpha hCG cDNA was purified after a single round of plaque purification. Insect cell culture infected with vAc alpha hCG, secreted high levels of hCG which was biologically active.
authors.affiliation: National Institute of Immunology, Shahid Jeet Singh Marg, New Delhi, India.
authors.forename: B
authors.initials: B
authors.lastname: Nakhai
authors.forename: P
authors.initials: P
authors.lastname: Sridhar
authors.forename: G P
authors.initials: GP
authors.lastname: Talwar
authors.forename: S E
authors.initials: SE
authors.lastname: Hasnain
mesh_terms: D016367:Baculoviridae / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005822:Genetic Vectors; D015292:Glycoprotein Hormones, alpha Subunit / Q000235:genetics*; D006801:Humans; D015183:Restriction Mapping
chemical_list: D015292:Glycoprotein Hormones, alpha Subunit; D004247:DNA