Platelet-activating factor stimulates expression of IL-1 beta mRNA in THP-1 cells.
A human monocytic cell line (THP-1) was used to study the effects of PAF (platelet-activating factor) on the expression of IL-1 beta mRNA. THP-1 cells were incubated with 10 pM PAF in the presence or absence of 0.1 microgram/mL endotoxin for 4 hr, after which cytoplasmic RNA was extracted and subjected to Northern hybridizations. PAF, alone and in combination with endotoxin, caused an increase in mRNA levels for IL-1 beta. The magnitude of the effects of PAF on IL-1 beta mRNA levels matched closely the effects seen at the level of protein synthesis, suggesting that the effects of PAF on IL-1 beta release may result largely from its effects on IL-1 beta mRNA levels.
Barthelson
R A
RA
Department of Medicine, University of California San Francisco.
Valone
F H
FH
D001345:Autoradiography; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D009000:Monocytes / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism*
D007375:Interleukin-1; D010972:Platelet Activating Factor; D012333:RNA, Messenger; D012313:RNA