[Synthesis and immunochemical properties of oligopeptides--fragments of capsid proteins of the hepatitis A virus].
The hepatitis A virus (HAV) capsid protein VP1, VP2 and VP3 are exposed at the virion surface and should therefore contain antigenic determinants. Algorithms for hydrophilicity, antigenicity and flexibility were used to predict probable antigenic sites. Synthesis of 7- to 23-membered overlapping peptides from seven sites, viz., 1-11, 1-17, 2-33, 11-25, 73-82, 76-86, 98-109, 98-112, 102-107, 102-108, 108-127, 113-123, 118-140, 276-298 from VP1, 42-62 from VP2, 76-85 from VP3, and 1-23 from VP4, was performed by various solid-phase methods. Free peptides and their conjugates with different carriers were used for immunization and study of antigenicity. The peptides did not interact with antibodies to the hepatitis A virus, whereas their conjugates did not induce the formation of anti-HAV-antibodies.
Chikin
L D
LD
Kozhich
A T
AT
Ivanov
V S
VS
Ivanov
V T
VT
Nastashenko
T A
TA
Kusov
Iu Iu
IuIu
Balaian
M S
MS
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000276:immunology; D002213:Capsid / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D006507:Hepatovirus / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000378:metabolism
D000956:Antigens, Viral; D000939:Epitopes; D010455:Peptides
Sintez i immunokhimicheskie svoĭstva oligopeptidov--fragmentov kapsidnykh belkov virusa gepatita A.