[The autocrine regulation of proliferation in cell cultures. I. A study of the heparin-binding factors with growth-stimulating activity contained in media conditioned with transformed rat fibroblasts].
A study was made of the medium conditioned by spontaneously transformed rat embryo fibroblasts of line Rec1-sf, which are capable of unlimited reproduction in medium free of serum and of other endogenous growth factors (c-medium). Addition of c-medium to stationary cultures of nontransformed rat embryo fibroblasts (REF), spontaneously transformed REF (line Rec1), and cells of Rec1-sf stimulated the incorporation of 3H-thymidine by 1.5-6 times. SDS-polyacrilamide-gel electrophoresis of proteins, marked by 35S-metionine of c-medium of the cell line Rec 1-sf, demonstrated that this medium had proteins with molecular mass from 10 to 110 kDa. The fractionating divisible by 100 ultra-concentrates of c-medium with utilization of heparin-sepharose allowed to isolate two types of heparin-binding proteins. The proteins of the first type took about 5% of all the proteins of c-medium; they were eluted with 1.1 M NaCl and stimulated the incorporation of 3H-thymidine in REF, Rec1 and Rec1-sf cultures by 1.3-1.9 times. The second type proteins took about 1% of all the proteins of c-medium and were eluted with 2M NaCl, and, like the main endogenic basic growth factor of fibroblasts, stimulated the incorporation of 3H-thymidine into REF and Rec1-sf, but not into the culture of Rec1 line cells. The results obtained are discussed in terms of a hypothesis of autocrine regulation of cell proliferation.
Goĭlo
T A
TA
Kostrominova
T Iu
TIu
Turilova
V I
VI
Pleskach
V A
VA
D000818:Animals; D001345:Autoradiography; D002455:Cell Division / Q000502:physiology; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured / Q000502:physiology; D003470:Culture Media / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D016220:Fibroblast Growth Factor 1 / Q000032:analysis / Q000502:physiology*; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology; D008970:Molecular Weight; D051381:Rats
D003470:Culture Media; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1
Autokrinnaia reguliatsiia proliferatsii v kul'turakh kletok. I. Issledovanie geparinsviazyvaiushchikh faktorov s rostostimuliruiushcheĭ aktivnost'iu, soderzhashchikhsia v sredakh, konditsionirovannykh transformirovannymi fibroblastami krysy.