Expression of the large myelin-associated glycoprotein isoform during the development in the mouse peripheral nervous system.
The developmental maximum expression of the large myelin-associated glycoprotein isoform (L-MAG) protein prior to that of the small myelin-associated glycoprotein isoform (S-MAG) in both the central and peripheral nervous systems (CNS, PNS) in mice was shown by immunoblotting techniques using specific antibodies to the L-MAG protein and the S-MAG protein. Both the L-MAG protein and the S-MAG protein were expressed earlier in the PNS than in the CNS, which reflects earlier myelination in the PNS. The peak of the L-MAG protein concentration was 8 days in the sciatic nerve and 15 days in the brainstem. The concentration of the S-MAG protein in the sciatic nerve reached a peak at 15 days, whereas in the brainstem it increased rapidly between 15 and 20 days and gradually thereafter. Thus, the preceding maximum expression of the L-MAG during active myelination in the PNS demonstrated here as well as in the CNS strongly suggests an important role for L-MAG in myelin formation.
Inuzuka
T
T
Department of Neurology, Niigata University, Japan.
Fujita
N
N
Sato
S
S
Baba
H
H
Nakano
R
R
Ishiguro
H
H
Miyatake
T
T
D000375:Aging; D000818:Animals; D001933:Brain Stem / Q000254:growth & development* / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D015151:Immunoblotting; D051379:Mice; D008815:Mice, Inbred Strains; D009185:Myelin Proteins / Q000032:analysis / Q000235:genetics*; D018992:Myelin-Associated Glycoprotein; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D012584:Sciatic Nerve / Q000254:growth & development* / Q000502:physiology
D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D012333:RNA, Messenger