Scyllatoxin, a blocker of Ca(2+)-activated K+ channels: structure-function relationships and brain localization of the binding sites.
Chemical modifications of scyllatoxin (leiurustoxin I) have shown that two arginines in the sequence, Arg6 and Arg13, are essential both for binding to the Ca(2+)-activated K+ channel protein and for the functional effect of the toxin. His31 is important both for the binding activity of the toxin and for the induction of contractions on taenia coli. However, although its iodination drastically decreases the toxin activity, it does not abolish it. Chemical modification of lysine residues or of Glu27 does not significantly alter toxin binding, but it drastically decreases potency with respect to contraction of taenia coli. The same observation has been made after chemical modification of the lysine residues. The brain distribution of scyllatoxin binding sites has been analyzed by quantitative autoradiographic analysis. It indicates that apamin (a bee venom toxin) binding sites are colocalized with scyllatoxin binding sites. The results are consonant with the presence of apamin/scyllatoxin binding sites associated with Ca(2+)-activated K+ channels. High-affinity binding sites for apamin can be associated with very-high-affinity (less than 70 pM), high-affinity (approximately 100-500 pM), or moderate-affinity (greater than 800 pM) binding sites for scyllatoxin.
Auguste
P
P
Institute de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
Hugues
M
M
Mourre
C
C
Moinier
D
D
Tartar
A
A
Lazdunski
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001030:Apamin / Q000037:antagonists & inhibitors* / Q000378:metabolism; D001345:Autoradiography; D001665:Binding Sites; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects / Q000502:physiology; D006168:Guinea Pigs; D006639:Histidine; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007700:Kinetics; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D009928:Organ Specificity; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000378:metabolism*; D012604:Scorpion Venoms / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D007457:Iodine Radioisotopes; D015221:Potassium Channels; D011950:Receptors, Cholinergic; D012604:Scorpion Venoms; C070000:scyllatoxin receptor; C056574:leiurotoxin I; D001030:Apamin; D006639:Histidine; D002118:Calcium