Cellular and tissue distribution of a single-strand-specific nuclease.
1. Specific antibodies which were raised against a single-strand-specific nuclease isolated from rat liver microsomes were used for characterizing this enzyme and determining its cellular and tissue distribution. 2. The single-strand-specific nuclease does not show any homology with other known nucleolytic enzymes. 3. It is mainly localized in microsomes and cytosol; traces of it are also found in nuclei, but it could not be detected in mitochondria. 4. Using the same specific antibodies we attempted to detect this nuclease in some other tissues which exhibit high metabolic rates, namely kidneys, heart and spleen. 5. Thus, with the aid of immunological techniques we were able to determine that at least part of the total poly(U) nucleolytic activity observed in kidney and heart is due to a nuclease immunologically identical to the enzyme under study. Kidneys were the best source for this enzyme.
Vavatsi
N A
NA
Department of Biological Chemistry, School of Medicine, Aristotelian University of Thessaloniki, Greece.
Kouidou
S A
SA
Triantos
A A
AA
Kavoukopoulos
E M
EM
Papageorgiou
G E
GE
Trakatellis
A C
AC
D000818:Animals; D000906:Antibodies; D003600:Cytosol / Q000201:enzymology; D004277:DNA, Single-Stranded / Q000378:metabolism; D003851:Deoxyribonucleases / Q000276:immunology / Q000378:metabolism*; D004720:Endonucleases / Q000276:immunology / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D012313:RNA / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases / Q000276:immunology / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000201:enzymology
D000906:Antibodies; D004277:DNA, Single-Stranded; D012313:RNA; D003851:Deoxyribonucleases; D004720:Endonucleases; D012260:Ribonucleases; C054500:single-strand-specific nuclease, endoplasmic reticulum