Rapid method for isolation and staining of bacterial lipopolysaccharide.
Classically bacterial lipopolysaccharide (LPS) purification and silver staining take several days. We designed a simple and fast method for LPS isolation which when combined with silver staining using Pharmacia PhastSystem both can be completed in few hours. The purity of LPS isolated by this simple method may not be comparable to that by the phenol-water method hence we recommend this rapid isolation and staining procedures for simple and fast study of LPS patterns in gels.
al-Hendy
A
A
Department of Medical Microbiology, Turku University, Finland.
Toivanen
P
P
Skurnik
M
M
D004591:Electrophoresis, Polyacrylamide Gel; D005590:Fractional Precipitation; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D012486:Salmonella typhimurium / Q000737:chemistry; D012834:Silver; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling / Q000379:methods; D015007:Yersinia / Q000737:chemistry
D008070:Lipopolysaccharides; D012967:Sodium Dodecyl Sulfate; D012834:Silver