Combination of neutron activation analysis, tracer techniques, and biochemical methods in the investigation of selenium metabolism.
In several studies on rats, the metabolism of selenium was investigated. The quantitative determination of the element was carried out by instrumental neutron activation analysis. For in vivo tracer experiments, 75Se-labeled selenium compounds were used. In addition to these methods, procedures for the measurement of the selenoenzyme glutathione peroxidase, and for the investigation of other selenoproteins, were applied. In this way, information on the specific pools and sites of action of the element, on biologically important selenoproteins and the regulation of the selenium metabolism, was obtained.
Behne
D
D
Hahn-Meitner-Institut Berlin, FRG.
Scheid
S
S
Hilmert
H
H
Gessner
H
H
Gawlik
D
D
Kyriakopoulos
A
A
D000818:Animals; D005979:Glutathione Peroxidase / Q000378:metabolism; D009501:Neutron Activation Analysis / Q000379:methods*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D012643:Selenium / Q000032:analysis / Q000172:deficiency / Q000378:metabolism*; D012644:Selenium Radioisotopes; D051140:Selenoproteins; D014018:Tissue Distribution
D011506:Proteins; D012644:Selenium Radioisotopes; D051140:Selenoproteins; D005979:Glutathione Peroxidase; D012643:Selenium