Centromere detection in vinblastine- and radiation-induced micronuclei of cytokinesis-blocked mouse cells by using in situ hybridization with a mouse gamma (major) satellite DNA probe.
Non-isotopic in situ hybridization using a mouse gamma (major) satellite probe DNA was applied to detect centromeres in micronuclei, which were induced in vitro in mouse liver cells by ionizing radiation and by vinblastine sulfate. In a cytokinesis-blocked micronucleus assay a dose-dependent induction of micronuclei was found for both agents. After vinblastine exposure the observed micronuclei showed centromere-positive hybridization signals in an order of magnitude of 70-90%, but after radiation exposure the magnitude was only 10-20%. Since the in situ hybridization technique detects centromeric DNA directly, it can be used in a cytokinesis-blocked micronucleus assay for a rapid and reliable discrimination between aneuploidy-inducing and clastogenic agents.
Salassidis
K
K
Institut für Strahlenbiologie, GSF-Forschungszentrum für Umwelt and Gesundheit, Neuherberg, Germany.
Huber
R
R
Zitzelsberger
H
H
Bauchinger
M
M
D000818:Animals; D015197:Carcinogenicity Tests / Q000379:methods*; D002460:Cell Line; D002503:Centromere; D015342:DNA Probes / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D051379:Mice; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D008856:Microscopy, Fluorescence; D009152:Mutagenicity Tests / Q000379:methods*; D009693:Nucleic Acid Hybridization; D014747:Vinblastine / Q000633:toxicity
D015342:DNA Probes; D004276:DNA, Satellite; D014747:Vinblastine