Regulation of MHC molecules on MBP positive oligodendrocytes in mice by IFN-gamma and TNF-alpha.
The expression of class I and class II histocompatibility antigens by myelin basic protein (MBP)-positive oligodendrocytes, in response to exogenous cytokines, has been investigated in vitro. It has been found that interferon-gamma (IFN-gamma), although capable of class I induction, does not induce class II on oligodendrocytes. Furthermore, tumour necrosis factor-alpha (TNF-alpha), which was shown to induce class I MHC on other neural cells, failed to induce class I on oligodendrocytes. A combination of IFN-gamma and TNF-alpha also failed to facilitate the expression of class II antigens on oligodendrocytes, nor did it amplify the expression of class I seen with IFN-gamma alone. Thus it appears that MBP+ murine oligodendrocytes are refractory to class II induction, and express class I in response to IFN-gamma but not TNF-alpha. The differential regulation and class of MHC expression may have implications in terms of the initiation and targeting of immune responses directed toward the oligodendrocyte.
Turnley
A M
AM
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Australia.
Miller
J F
JF
Bartlett
P F
PF
D001921:Brain / Q000276:immunology*; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007371:Interferon-gamma / Q000494:pharmacology*; D008285:Major Histocompatibility Complex; D004676:Myelin Basic Protein / Q000032:analysis*; D009836:Oligodendroglia / Q000187:drug effects / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D004676:Myelin Basic Protein; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma