title: A position effect on the time of replication origin activation in yeast.
abstract: The chromosomes of eukaryotes are characterized by the mosaic nature of their replication--large regions of DNA that replicate early in S phase are interspersed with regions that replicate late. This pattern of early and late synthesis appears to be the consequence of a temporal program that activates replication origins at different times. The basis of this temporal regulation in the yeast S. cerevisiae has been investigated by changing the chromosomal locations of two origins, one activated early in the S phase (ARS1) and one activated late (ARS501). We show that the cis-acting information controlling time of activation can be separated from the element that determines origin function. For the ARS501 origin, late activation appears to be a consequence of its proximity to the telomere.
authors.affiliation: Department of Genetics, University of Washington, Seattle 98195.
authors.forename: B M
authors.initials: BM
authors.lastname: Ferguson
authors.forename: W L
authors.initials: WL
authors.lastname: Fangman
mesh_terms: D015825:Chromosomes, Fungal / Q000502:physiology; D004261:DNA Replication / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D000072235:Mating Factor; D010455:Peptides / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016615:Telomere / Q000502:physiology
chemical_list: D004271:DNA, Fungal; D010455:Peptides; D000072235:Mating Factor