title: Increased cellular growth of FRTL-5 thyroid cells mediated by discontinuous stimulation with bTSH.
abstract: Previous reports showed pulsatile secretion of TSH in man. Therefore, we investigated the effect of discontinuous versus continuous TSH stimulation on the cellular level of FRTL-5 thyroid cells, namely the expression of a nuclear proliferation antigen (NPAg). The expression of this antigen correlates linearly with the 3H-thymidine incorporation and is a marker for cellular growth. The FRTL-5 cells were stimulated for 4 hours with bTSH (0.01-1.0 U/l). Compared to continuously stimulated cell cultures the discontinuous stimulation of FRTL-5 cells with bTSH showed a significant higher rate of NPAg expression, i.e. cellular growth.
authors.affiliation: Klinik für Innere Medizin, Medizinische Universität zu Lübeck, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Klugmann
authors.forename: I
authors.initials: I
authors.lastname: Mons
authors.forename: B E
authors.initials: BE
authors.lastname: Wenzel
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D009687:Nuclear Proteins / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013972:Thyrotropin / Q000494:pharmacology*
chemical_list: D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D013972:Thyrotropin