title: Binding of cloned S-fimbriated E. coli to human buccal epithelial cells--different inhibition of binding by neonatal saliva and adult saliva.
abstract: Investigations were carried out on the adhesion of cloned S-fimbriated E. coli, labelled with fluoresceinisothiocyanate (FITC) to human buccal epithelial cells. Fluorescence microscopic analysis revealed binding of bacteria to 75-95% of epithelial cells. Inhibition experiments with fetuin, alpha 1-acid glycoprotein and N-acetyl neuraminic acid confirmed the specificity of bacterial binding to sialoglycoproteins. Further studies using saliva as an inhibitor resulted in a 4-5 times stronger binding inhibition by newborn saliva in comparison to adult saliva coinciding with a 4-5 times higher content of total N-acetyl neuraminic acid in samples of newborn saliva. In Western blot analysis sialoglycoprotein bands with a molecular weight greater than 200 kD reacting with wheat germ agglutinin (WGA), were only identified in samples of newborn saliva. These bands are classified as mucins on account of molecular weight and staining. These data suggest that saliva mucins could represent a major defense mechanism against bacterial infections at a stage of ontogeny where the secretory IgA-system is not yet developed.
authors.affiliation: Universitäts-Kinderklinik, Düsseldorf.
authors.forename: H
authors.initials: H
authors.lastname: Schroten
authors.forename: A
authors.initials: A
authors.lastname: Wolske
authors.forename: R
authors.initials: R
authors.lastname: Plogmann
authors.forename: F G
authors.initials: FG
authors.lastname: Hanisch
authors.forename: J
authors.initials: J
authors.lastname: Hacker
authors.forename: G
authors.initials: G
authors.lastname: Uhlenbruck
authors.forename: V
authors.initials: V
authors.lastname: Wahn
mesh_terms: D000328:Adult; D001422:Bacterial Adhesion / Q000187:drug effects; D015153:Blotting, Western; D002478:Cells, Cultured; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006021:Glycophorins / Q000494:pharmacology; D006801:Humans; D007231:Infant, Newborn; D008856:Microscopy, Fluorescence; D009061:Mouth Mucosa / Q000166:cytology / Q000382:microbiology*; D009439:Neuraminidase / Q000494:pharmacology; D009961:Orosomucoid / Q000494:pharmacology; D012463:Saliva / Q000737:chemistry / Q000502:physiology*; D012794:Sialic Acids / Q000032:analysis / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000494:pharmacology
chemical_list: D006021:Glycophorins; D009961:Orosomucoid; D012794:Sialic Acids; D000509:alpha-Fetoproteins; D009439:Neuraminidase