Isolation and amino terminal sequence of beta-trace, a novel protein from human cerebrospinal fluid.
beta-Trace, a 23.5 kDa glycoprotein of unknown biological functions, is present in all body fluids tested. It is found in higher concentration in human seminal fluid and cerebrospinal fluid (CSF) than in serum. A one-step procedure for the isolation of beta-trace from pooled CSF is described, by affinity chromatography using a specific antibody made against beta-trace. Amino terminal sequence analysis yields the sequence A P E A Q V S V Q P N F Q Q D K F L G with no homology to known proteins, indicating that beta-trace is a novel CSF protein.
Kuruvilla
A P
AP
Department of Neurology, New York University Medical Center, NY 10016.
Hochwald
G M
GM
Ghiso
J
J
Castaño
E M
EM
Pizzolato
M
M
Frangione
B
B
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D001609:Beta-Globulins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002556:Cerebrospinal Fluid Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D006801:Humans; D007114:Immunization; D015151:Immunoblotting; D019746:Intramolecular Oxidoreductases; D054834:Lipocalins; D008969:Molecular Sequence Data; D011817:Rabbits
D000906:Antibodies; D001609:Beta-Globulins; D002556:Cerebrospinal Fluid Proteins; D054834:Lipocalins; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase