Lectin staining of peritoneal mesothelial cells in vitro.
A survey of lectin-binding specificities present on rodent and human mesothelial cells propagated and maintained in tissue culture was made using fluorescein isothiocynate conjugated (FITC) lectins. Rodent and human cells exhibited cell-associated fluorescence following exposure to the FITC-lectins from C. ensiformis, T. vulgaris, A. hypogaea, E. cristagalli and B. simplicifolia, but not with lectins from G. max and D. biflorus. Rodent cells were also positive for FITC-M. pomifera lectin binding. Human, but not rodent, cells were positive for FITC-T. purpureas lectin binding. Exposure of rabbit mesothelial cells in vitro to FITC-lectins that bound to the cell surface resulted in the appearance of discrete loci of putatively intracellular fluorescence. Exposure of cells to ferritin-labelled T. vulgaris lectin at 37 degrees C for as little as 7.5 minutes resulted in the appearance of ferritin-size particles in intracellular vesicles. These results demonstrate 1. the presence of lectin-binding sites in and on peritoneal mesothelial cells from rodents and humans and 2. a possible role of such sites in mediating the entry of lectin-like endogenous molecules into the vacuolar apparatus of these cells.
Hjelle
J T
JT
Department of Basic Sciences, University of Illinois College of Medicine, Peoria 61656.
Golinska
B T
BT
Waters
D C
DC
Steidley
K R
KR
Miller
M A
MA
McCarroll
D R
DR
Dobbie
J W
JW
D000818:Animals; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005293:Ferritins; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008856:Microscopy, Fluorescence; D010537:Peritoneum; D011817:Rabbits; D051381:Rats; D013194:Staining and Labeling
D005452:Fluoresceins; D037102:Lectins; D005293:Ferritins; D016650:Fluorescein-5-isothiocyanate