[Quantitative determination of the carbohydrate determinants of O-antigens of Yersinia pseudotuberculosis and Pseudomonas fluorescens].
Interactions of O-specific polysaccharides, LPS and LPS-protein complex with specific antibodies isolated on a polysaccharide-substituted immunoadsorbent gel and monovalent Fab-fragments of the antibodies were investigated. The association constants and quantity of Fab-fragments bound to these antigens were established. The interaction of the LPS and LPS-protein complexes was shown to depend considerably on concentration of antigens, and only 10-15% of carbohydrate determinants are accessible for binding with antibodies.
Naberezhnykh
G A
GA
Solov'eva
T F
TF
Ovodov
Iu S
IuS
D000209:Acute-Phase Proteins; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D002241:Carbohydrates / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D007128:Immunoglobulin Fragments / Q000276:immunology; D008562:Membrane Glycoproteins; D019081:O Antigens; D011551:Pseudomonas fluorescens / Q000276:immunology*; D015011:Yersinia pseudotuberculosis / Q000276:immunology*; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D002241:Carbohydrates; D002352:Carrier Proteins; D000939:Epitopes; D007128:Immunoglobulin Fragments; D008562:Membrane Glycoproteins; D019081:O Antigens; D000099231:Lipopolysaccharide-Binding Protein
Kolichestvennoe opredelenie uglevodnykh determinant O-antigenov Yersinia pseudotuberculosis i Pseudomonas fluorescens.