Succinate-ubiquinone reductase linked recycling of alpha-tocopherol in reconstituted systems and mitochondria: requirement for reduced ubiquinone.
Studies have demonstrated that accumulation of mitochondrial tocopheroxyl radical, the primary oxidation product of alpha-tocopherol, accompanies rapid consumption of tocopherol. Enzyme-linked electron flow lowers both the steady-state concentration of the radical and the consumption of tocopherol. Reduction of tocopheroxyl radical by a mitochondrial electron carrier(s) seems a likely mechanism of tocopherol recycling. Succinate-ubiquinone reductase (complex II) was incorporated into liposomes in the presence of tocopherol and ubiquinone-10. After inducing formation of tocopheroxyl radical, it was possible to show that reduced ubiquinone prevents radical accumulation and tocopherol consumption. There was no evidence of direct reduction of tocopheroxyl radical by succinate-reduced complex II. These reactions were also measured using ubiquinone-1 and alpha-C-6-chromanol (2,5,7,8-tetramethyl-2-(4'-methylpentyl)-6-chromanol) which are less hydrophobic analogues of ubiquinone-10 and alpha-tocopherol. Mitochondrial membranes were made deficient in ubiquinone but sufficient in alpha-tocopherol and were reconstituted with added quinone. With these membranes it was shown that mitochondrial enzyme-linked reduction of ubiquinone protects alpha-tocopherol from consumption, and there is a requirement for ubiquinone. This complements the observations made in liposomes and we propose that reduced mitochondrial ubiquinones have a role in alpha-tocopherol protection, presumably through efficient reduction of the tocopheroxyl radical.
Maguire
J J
JJ
Applied Science Division, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
Kagan
V
V
Ackrell
B A
BA
Serbinova
E
E
Packer
L
L
D000818:Animals; D042963:Electron Transport Complex II; D005609:Free Radicals; D007425:Intracellular Membranes / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008930:Mitochondria, Liver / Q000737:chemistry* / Q000201:enzymology; D009097:Multienzyme Complexes / Q000737:chemistry*; D010088:Oxidoreductases / Q000737:chemistry*; D011510:Proteolipids / Q000737:chemistry; D051381:Rats; D013367:Submitochondrial Particles / Q000737:chemistry; D013385:Succinate Dehydrogenase / Q000737:chemistry*; D014451:Ubiquinone / Q000737:chemistry* / Q000378:metabolism; D014810:Vitamin E / Q000031:analogs & derivatives / Q000737:chemistry*
D005609:Free Radicals; D008081:Liposomes; D009097:Multienzyme Complexes; D011510:Proteolipids; D014451:Ubiquinone; D014810:Vitamin E; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase