Detection and identification of Treponema hyodysenteriae by using oligodeoxynucleotide probes complementary to 16S rRNA.
Oligodeoxynucleotide probes (17 and 28 bases long) complementary to a unique region of Treponema hyodysenteriae 16S rRNA were developed. These probes bound specifically to partially purified rRNA and whole-cell rRNA of T. hyodysenteriae. No binding to partially purified rRNA or whole-cell rRNA of Treponema innocens, Treponema succinifaciens, Treponema bryantii, or Escherichia coli occurred under stringent conditions. The 28-base probe was 5 to 10 times more sensitive than the 17-base probe when hybridized with T. hyodysenteriae rRNA. The 28-base probe detected T. hyodysenteriae in the feces of experimentally inoculated pigs exhibiting clinical signs of swine dysentery.
Jensen
N S
NS
Physiopathology Research Unit, U.S. Department of Agriculture, Ames, Iowa 50010.
Casey
T A
TA
Stanton
T B
TB
D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D004403:Dysentery / Q000175:diagnosis / Q000662:veterinary; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis; D014208:Treponema / Q000235:genetics* / Q000302:isolation & purification; D014211:Treponemal Infections / Q000175:diagnosis / Q000662:veterinary
D015342:DNA Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S