Frequent occurrence of Ha-rasl allelic deletion in human ovarian adenocarcinomas.
Fourteen human adenocarcinoma specimens were analyzed for somatic abnormalities affecting genes of the ras family. No amplification of the 3 ras genes was detected. Allelic deletion of the Ha-rasl gene (11p15.5) was found to be a very common abnormality in human ovarian adenocarcinomas (4 out of 7 informative cases). However, in these neoplasm deletion of a presumed normal Ha-rasl allele is not a contributory factor in strengthening the tumorigenic effect of a mutated allele. More probably, Ha-rasl allelic losses are markers of larger chromosomal deletions. Analyses at gamma globin loci (11p15.5) and int-2 locus (11q13) provided evidence that the deletions may extend from Ha-rasl locus towards the centromere but never involve loss of the entire chromosome 11. These findings may suggest that a putative tumor suppressor gene closely linked to Ha-rasl in 11p15.5 is involved in ovarian cancerogenesis.
Viel
A
A
Division of Experimental Oncology 1, Centro di Riferimento Oncologico, Aviano, Pordenone, Italy.
De Pascale
L
L
Toffoli
G
G
Tumiotto
L
L
Miotto
E
E
Boiocchi
M
M
D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D000483:Alleles; D002872:Chromosome Deletion; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics*; D005719:gamma-Globulins / Q000235:genetics
D004273:DNA, Neoplasm; D005719:gamma-Globulins