Does insulin-like growth factor I (IGF-1) trigger the cell body reaction in the rat sciatic nerve?
Regeneration was measured after the infliction of a crush lesion on rat sciatic nerves which 4 days earlier had been subjected to a distal conditioning transection. Such nerves exhibited an increased outgrowth of nerve fibers as compared to nerves subjected to a single crush lesion. This increased outgrowth could be prevented, if the nerve was locally perfused around the site of the transection during the 4 days conditioning interval, with cycloheximide, actinomycin D and vinblastine, inhibitors of protein-, RNA-synthesis and retrograde axonal transport, respectively. The inhibitory effect of cycloheximide could be overcome by simultaneous perfusion with insulin-like growth factor I (IGF-1). The results suggest that proteins including IGF-1 which are synthesised locally around a nerve lesion and then transported retrogradely could trigger regenerative events in the neuronal cell body.
Kanje
M
M
Department of Animal Physiology, University of Lund, Sweden.
Skottner
A
A
Lundborg
G
G
Sjöberg
J
J
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D012584:Sciatic Nerve / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D009419:Nerve Tissue Proteins; D012313:RNA; D007334:Insulin-Like Growth Factor I; D004247:DNA