Parotid secretion of fluid, amylase and kallikrein during reflex stimulation under normal conditions and after acute administration of autonomic blocking agents in man.
The purpose of this work was to study the effect of graded mechanical and gustatory stimulation on the secretion of the acinar products fluid and amylase and the ductal product kallikrein from the human parotid gland (n = 9). The involvement of parasympathetic and sympathetic nerves in the salivary reflexes was subsequently examined using receptor blocking agents (n = 4). Chewing elevated the secretion of all products as compared to rest (P less than 0.013). When increasing the length of the chewing object, secretion of fluid (P less than 0.013), but not enzymes, further increased. The shift from mechanical to gustatory stimulation with 0.5% citric acid enhanced significantly the secretion of amylase and kallikrein (P less than 0.009), while application of 5.0% citric acid increased the secretion of both acinar products (P less than 0.009) more than kallikrein. A differentiated reflex control of salivation both with regard to input and output was thereby indicated. The muscarinic-cholinergic antagonist oxyphencyclimin reduced median fluid secretion between 54 and 76% depending on the stimuli. During citric acid stimulation, but not during chewing, fluid secretion was reduced about 40% by the beta 1-adrenergic antagonist metoprolol, and about 20% by the alpha 1-adrenergic antagonist prazosin. Median amylase secretion was reduced 30% during chewing and 75% during gustatory stimulation by metoprolol. It was concluded that the masticatory-salivary reflex mainly activated parasympathetic pathways producing saliva of low protein content.(ABSTRACT TRUNCATED AT 250 WORDS)
Jensen
J L
JL
Department of Physiology and Biochemistry, Faculty of Dentistry, University of Oslo, Norway.
Brodin
P
P
Berg
T
T
Aars
H
H
D000328:Adult; D000681:Amylases / Q000378:metabolism*; D001341:Autonomic Nervous System / Q000187:drug effects / Q000502:physiology*; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D005260:Female; D006801:Humans; D007610:Kallikreins / Q000378:metabolism*; D008297:Male; D008790:Metoprolol / Q000494:pharmacology; D010276:Parasympatholytics / Q000494:pharmacology*; D010306:Parotid Gland / Q000378:metabolism*; D011224:Prazosin / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D012463:Saliva / Q000378:metabolism*; D012472:Salivation / Q000187:drug effects / Q000502:physiology*; D012636:Secretory Rate / Q000187:drug effects
D002951:Citrates; D010276:Parasympatholytics; D011743:Pyrimidines; D019343:Citric Acid; C100201:oxyphencyclimine; D000681:Amylases; D007610:Kallikreins; D008790:Metoprolol; D011224:Prazosin