title: Purification and characterization of two basic beta-1,3-glucanases induced in Colletotrichum lindemuthianum-infected bean seedlings.
abstract: Two beta-1,3-glucanases which are rapidly induced in the incompatible interaction between bean (cv. Processor) and Colletotrichum lindemuthianum race beta were purified to homogeneity. Characterization of the two enzymes, GE1 and GE2, showed that they both had a basic isolectric point and a similar molecular weight (36,500 for GE1 and 36,000 for GE2), but differed in their pH optimum, thermal stability, and specific activity. GE2 was present in higher amounts but was shown to be less active than GE1 against laminarin and fungal cell walls isolated from race beta of the fungus. Both enzymes were specific for beta-1,3 linkages and showed a strict endolytic mode of action. Further characterization of GE2 was achieved by amino acid sequence analysis of tryptic peptides; the degree of homology shared with other basic beta-1,3-glucanases depended on the plant source. A time-course study showed that GE1 and GE2 were increased during infection. They were also induced by fungal elicitors, thereby indicating that they originate from the host.
authors.affiliation: Centre de Physiologie Végétale, Université Paul Sabatier, U. A. CNRS No. 241, Toulouse, France.
authors.forename: J H
authors.initials: JH
authors.lastname: Daugrois
authors.forename: C
authors.initials: C
authors.lastname: Lafitte
authors.forename: J P
authors.initials: JP
authors.lastname: Barthe
authors.forename: C
authors.initials: C
authors.lastname: Faucher
authors.forename: A
authors.initials: A
authors.lastname: Touze
authors.forename: M T
authors.initials: MT
authors.lastname: Esquerre-Tugaye
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D004790:Enzyme Induction; D004795:Enzyme Stability; D007887:Fabaceae / Q000201:enzymology* / Q000382:microbiology; D043326:Glucan 1,3-beta-Glucosidase; D007700:Kinetics; D003904:Mitosporic Fungi / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010946:Plants, Medicinal; D012689:Sequence Homology, Nucleic Acid; D001617:beta-Glucosidase / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase