How does ppGpp affect translational accuracy in the stringent response?
With an in vitro poly(Phe) synthesis system we have tested recent models concerning translational accuracy in the stringent response during aminoacid starvation. We have found that cognate, deacylated tRNA of very high concentrations is unable to block the A-site. No influence of EF-Tu.ppGpp on ribosomal proofreading has been found. Alternative mechanisms to keep translational errors low by the stringent response are discussed.
Rojas
A M
AM
Department of Molecular Biology, Uppsala University, Sweden.
Ehrenberg
M
M
D000596:Amino Acids / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D006159:Guanosine Tetraphosphate / Q000494:pharmacology*; D010452:Peptide Biosynthesis; D010444:Peptide Elongation Factor Tu / Q000494:pharmacology; D010455:Peptides; D014176:Protein Biosynthesis / Q000187:drug effects*; D012329:RNA, Bacterial / Q000235:genetics; D012360:RNA, Transfer, Phe / Q000235:genetics; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism
D000596:Amino Acids; D010455:Peptides; D012329:RNA, Bacterial; D012360:RNA, Transfer, Phe; C012001:polyphenylalanine; D006159:Guanosine Tetraphosphate; D010444:Peptide Elongation Factor Tu