title: Plasmodium berghei: in vivo generation and selection of karyotype mutants and non-gametocyte producer mutants.
abstract: We previously reported that karyotype and gametocyte-producer mutants spontaneously arose during in vivo asexual multiplication of Plasmodium berghei. Here we studied the rate of selection of these mutants in vivo. Gametocyte production and karyotype pattern were established at regular intervals during prolonged periods of asexual multiplication of clone 8417 of P. berghei. We found that karyotype mutants and mutants which do not produce gametocytes can replace the original high-producer parasites of clone 8417 within several weeks. The time at which mutants became predominant in the population in different experiments, however, differed greatly. Mutants with intermediate or low gametocyte production were not found. In experimentally mixed infections, containing parasites from two clones from different strains (clone 8417 of the ANKA strain; clone 1 of the K173 strain), high-producer parasites of clone 8417 were overgrown by parasites of the nonproducer clone. Nonproducer mutants from the originally high-producer clone 8417, however, were able to coexist with parasites of the nonproducer clone. These results demonstrate that in our experiments nonproducer parasites had a strong selective advantage during asexual multiplication compared to high producers. All karyotype mutants which became predominant in our experiments were nonproducers. In two experiments a change in karyotype coincided with the loss of gametocyte production which may suggest a causal relationship between these events.
authors.affiliation: Laboratory of Parasitology, University of Leiden, The Netherlands.
authors.forename: C J
authors.initials: CJ
authors.lastname: Janse
authors.forename: J
authors.initials: J
authors.lastname: Ramesar
authors.forename: F M
authors.initials: FM
authors.lastname: van den Berg
authors.forename: B
authors.initials: B
authors.lastname: Mons
mesh_terms: D000818:Animals; D005718:Gametogenesis / Q000235:genetics; D014644:Genetic Variation; D007621:Karyotyping; D008288:Malaria / Q000469:parasitology; D051379:Mice; D008938:Mitosis / Q000235:genetics; D009154:Mutation; D010962:Plasmodium berghei / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D012100:Reproduction, Asexual / Q000235:genetics