Characterization of Sendai virus-induced human placental trophoblast interferons.
Human placental trophoblast cultures produce a mixture of interferons (IFNs) when challenged with Sendai virus. High-performance dye-ligand and immunoaffinity chromatography of a trophoblast IFN (tro-IFN) preparation enabled the isolation of three antigenically distinct IFNs, alpha I, alpha II 1 and beta, with Mrs of 16K, 22K and 24K respectively, by reducing and non-reducing SDS-PAGE. The major IFN, responsible for 75% of the total antiviral activity, was tro-IFN-beta, with the remaining activity being due to tro-IFN-alpha I and tro-IFN-alpha II 1, as determined by an antiviral neutralization test using specific anti-human IFN antibodies. The antiviral activities of the tro-IFNs were stable at pH 2.0 for 24 h and tro-IFN-alpha II 1 and -beta were shown to be glycoproteins. The three tro-IFNs showed different antiviral activities when assayed on human and bovine cell species; tro-IFN-alpha I and alpha II 1 protected both human and bovine (MDBK) cells from virus infection, whereas tro-IFN-beta showed a high degree of species specificity, protecting only the human cell types tested.
Aboagye-Mathiesen
G
G
Danish Cancer Society, Department of Virus and Cancer, Aarhus C.
Tóth
F D
FD
Juhl
C
C
Nørskov-Lauritsen
N
N
Petersen
P M
PM
Zachar
V
V
Ebbesen
P
P
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002417:Cattle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007372:Interferons / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D009500:Neutralization Tests; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D014327:Trophoblasts / Q000276:immunology*
D000914:Antibodies, Viral; D007372:Interferons