Immunohistochemical demonstration of alpha 1-antichymotrypsin and alpha 1-antitrypsin in salivary gland pleomorphic adenomas of children.
Twenty-five benign pleomorphic adenomas of salivary glands in children were studied with immunohistochemical techniques in order to characterize the cell types comprising the epithelial and so-called "mesenchymal" regions of the tumors. The antisera against alpha 1-antichymotrypsin (alpha 1-ACT) and alpha 1-antitrypsin (alpha 1-AT) were used to stain in normal salivary gland tissue as well as in pleomorphic adenoma. In normal salivary glands, alpha 1-ACT was localized to the intercalated duct and serous acinar cells. On the other hand, there was positive staining for alpha 1-AT in the intercalated and striated duct cells. Twenty-five cases (100%) of pleomorphic adenomas in children displayed positivity to alpha 1-ACT staining and 22 cases (88%) showed a positive staining for alpha 1-AT. alpha 1-ACT staining was particularly intense in chondrocyte-like cells of 20 cases (80%), in inner tubular cells of 16 (64%) and cyst-lining cells of 12 (52%). The limited number of tumor cells which were called plasmatoid or hyaline cells and squamous epithelial cells, were positive for alpha 1-ACT. None of the outer tubular cells and hyalinous material was positively stained for alpha 1-ACT. A strong positive reaction for alpha 1-AT was observed in chondrocyte-like cells of 15 cases (60%). Inner tubular cells were positive for alpha 1-AT in 12 cases (48%), plasmatoid or hyaline cells in 10 (40%) and cyst-lining cells in 8 (35%). Squamous epithelial cells, clear cells, secretory product and hyalinous material were positive for alpha 1-AT in some cases. Chondroid matrix and myxoid stroma had no reaction with both antibodies. The biological role of alpha 1-ACT and alpha 1-AT with a wide immunohistochemical distribution in pleomorphic adenomas of children may be associated with a self regulating mechanism which inhibits degradation by tissue proteinases.
Takahashi
H
H
Department of Oral Pathology, Nagasaki University School of Dentistry.
Fujita
S
S
Tsuda
N
N
Tezuka
F
F
Okabe
H
H
D000236:Adenoma / Q000473:pathology* / Q000601:surgery; D002356:Cartilage / Q000473:pathology; D002648:Child; D004848:Epithelium / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D012016:Reference Values; D012468:Salivary Gland Neoplasms / Q000473:pathology* / Q000601:surgery; D012469:Salivary Glands / Q000166:cytology* / Q000473:pathology; D013194:Staining and Labeling; D000514:alpha 1-Antichymotrypsin / Q000032:analysis*; D000515:alpha 1-Antitrypsin / Q000032:analysis*
D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin