The immune response to anti-idiotype antibodies bearing an internal image epitope of tetanus toxin/toxoid. II. Comparison of the primary humoral immune response to xenogeneic Ab2 beta 1 and Ab2 beta 2 internal image anti-idiotype antibodies.
Mouse monoclonal (Ab1) anti-tetanus toxin/toxoid antibodies were used to raise Ab2 beta (tetanus toxin/toxoid internal image bearing) anti-idiotype antibodies in rabbits. Those rabbit serum antibodies (Ab2 beta) that did not bind to mouse serum proteins on an affinity column gave rise to an Ab3 anti-tetanus toxin/toxoid antibody response in mice. Rabbit serum antibodies that did bind to the affinity column, when eluted and used to inoculate mice also gave rise to an Ab3 anti-tetanus toxin/toxoid antibody response. It is suggested that one population of rabbit Ab2 beta anti-idiotype antibodies (unbound fraction) bears a partial or complete internal image of a tetanus epitope (Ab2 beta 1) while others (bound fraction) bear a complete or partial mirror image of a mouse immunoglobulin epitope as well (Ab2 beta 2).
Poskitt
D C
DC
Commonwealth Serum Laboratories, Research and Development Division, Melbourne, Australia.
Turnbull
S
S
Macdonald
L
L
Jean-François
M J
MJ
Yasmeen
D
D
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis; D000917:Antibody Formation; D002846:Chromatography, Affinity; D000939:Epitopes / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013744:Tetanus Toxin / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D013744:Tetanus Toxin; D013745:Tetanus Toxoid