Short-term changes in erythrocyte alpha-tocopherol content of vitamin E-deficient patients with cystic fibrosis.
Polyunsaturated fatty acids of biomembranes are a major target of lipid peroxidation. In vitamin E deficiency an efficient delivery of a high oral loading dose of all-rac-alpha-tocopheryl acetate to erythrocyte membranes could provide an early onset antioxidative effect. We investigated short-term changes in erythrocyte alpha-tocopherol after a single oral dose of 100 mg all-rac-alpha-tocopheryl acetate/kg in 10 vitamin E-deficient cystic fibrosis (CF) patients. Over 24 h, erythrocyte alpha-tocopherol increased 68% to 420% of preloading concentrations. With two exceptions, peak values were achieved 12 or 24 h after administration, which was 3-18 h later than peak plasma concentrations. Separate median-based curve estimates for the changes in erythrocyte alpha-tocopherol for five patients with and five without associated cholestatic liver disease were obtained. Cross-sectional test results revealed significantly lower erythrocyte alpha-tocopherol for the 9- and 24-h observations for patients with cholestatic liver disease compared with those without. Oral all-rac-alpha-tocopheryl acetate can be rapidly incorporated into erythrocyte membranes in vitamin E-deficient CF patients.
Winklhofer-Roob
B M
BM
Department of Pediatrics, University of Zurich, Switzerland.
Shmerling
D H
DH
Schimek
M G
MG
Tuchschmid
P E
PE
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002780:Cholestasis, Intrahepatic / Q000097:blood* / Q000150:complications; D003430:Cross-Sectional Studies; D003550:Cystic Fibrosis / Q000097:blood* / Q000150:complications; D004912:Erythrocytes / Q000737:chemistry*; D005500:Follow-Up Studies; D006801:Humans; D007223:Infant; D011897:Random Allocation; D014810:Vitamin E / Q000097:blood*; D014811:Vitamin E Deficiency / Q000097:blood* / Q000150:complications
D014810:Vitamin E