Organ and species specific differences in cytoskeletal protein profiles of cultured microvascular endothelial cells.
1. Using two-dimensional gel electrophoresis and immunoblotting techniques we systematically document the structural diversity of cytoskeletal proteins in tight and leaky cultured microvascular endothelial cells (MEC). Bovine pulmonary and eel rete mirabile MEC primarily express cytokeratins 8 and 19. Cytokeratins 8 and 18 were found to be prominent in rat pulmonary MEC. Bovine retinal MEC contained cytokeratins 8, 18 and 19. Bovine adrenal MEC contain vimentin as their sole intermediate filament protein. 2. Four principal actin isoforms were resolved in micro/macrovascular endothelial cells as well as in vascular smooth muscle cells. Retinal pericytes expressed three principal actin isoforms. 3. These results indicate that MEC are diverse, highly differentiated cells displaying a large repertoire of cytoskeletal protein profiles suited for specific tissue functions.
Dodge
A B
AB
Biological Research Center, Boston University, MA 02215.
Patton
W F
WF
Yoon
M U
MU
Hechtman
H B
HB
Shepro
D
D
D000199:Actins / Q000032:analysis; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000032:analysis*; D003893:Desmin / Q000032:analysis; D004524:Eels; D015180:Electrophoresis, Gel, Two-Dimensional; D004730:Endothelium, Vascular / Q000737:chemistry*; D007633:Keratins / Q000032:analysis; D008168:Lung / Q000098:blood supply; D008833:Microcirculation / Q000737:chemistry; D009928:Organ Specificity; D051381:Rats; D012171:Retinal Vessels / Q000737:chemistry; D013045:Species Specificity; D014746:Vimentin / Q000032:analysis
D000199:Actins; D003598:Cytoskeletal Proteins; D003893:Desmin; D014746:Vimentin; D007633:Keratins