The rat alpha 2-C4 adrenergic receptor gene encodes a novel pharmacological subtype.
A rat gene and brain cDNA (pA2d) encoding the homologue of the human alpha-C4 adrenergic receptor subtype were isolated and characterized. RNA blots indicate that this gene is expressed in brain, heart and kidney but not in lung, liver or pancreas. Yohimbine, WB-4101 and prasozin all exhibited high affinity for this receptor in binding studies. Clonidine was more potent and efficacious than norepinephrine in inhibiting forskolin-stimulated cAMP production in CHO cells expressing pA2d. Together, these data suggest that the alpha 2-C4 gene product defines a previously undescribed pharmacological subtype of alpha 2-adrenergic receptor.
Voigt
M M
MM
Laboratory of Molecular Biology, NINDS, Bethesda, MD 20817.
McCune
S K
SK
Kanterman
R Y
RY
Felder
C C
CC
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000037:antagonists & inhibitors; D004247:DNA / Q000235:genetics; D004146:Dioxanes / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D011224:Prazosin / Q000494:pharmacology; D012313:RNA / Q000032:analysis; D011869:Radioligand Assay; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D015016:Yohimbine / Q000378:metabolism / Q000494:pharmacology
D000317:Adrenergic alpha-Antagonists; D004146:Dioxanes; D011942:Receptors, Adrenergic, alpha; D005576:Colforsin; D015016:Yohimbine; D012313:RNA; D004247:DNA; D000242:Cyclic AMP; C010654:(2-(2',6'-dimethoxy)phenoxyethylamino)methylbenzo-1,4-dioxane; D011224:Prazosin