title: Primary culture of chicken bursal plical epithelium.
abstract: Plical epithelial cells were obtained by trypsin-EDTA treatment of chicken bursa of Fabricius and cultured in the presence of type IV collagen. The culture became confluent six to seven days after seeding. The grown cells showed a positive reaction for cytokeratin by immunostaining and had ultrastructural characteristics of the epithelial cells in vivo. The cell culture will be useful for parasitological and virological studies.
authors.affiliation: Nippon Institute for Biological Science, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nunoya
authors.forename: M
authors.initials: M
authors.lastname: Tajima
authors.forename: C
authors.initials: C
authors.lastname: Itakura
mesh_terms: D000818:Animals; D002060:Bursa of Fabricius / Q000166:cytology* / Q000648:ultrastructure; D002478:Cells, Cultured; D002645:Chickens / Q000033:anatomy & histology*; D003094:Collagen; D004492:Edetic Acid / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008854:Microscopy, Electron; D013047:Specific Pathogen-Free Organisms; D014357:Trypsin / Q000378:metabolism
chemical_list: D007633:Keratins; D003094:Collagen; D004492:Edetic Acid; D014357:Trypsin