title: [The direct demonstration of the tyrosine phosphorylation of epidermal growth factor receptors internalized in the endosomes of A431 cells].
abstract: Phosphorylation of the epidermal growth factor (EGF) receptors in endosomes isolated from A431 cells was studied using antiphosphotyrosin antibody (anti-P-Tyr). A431 cells were preincubated with EGF and then washed with acid buffer to remove surface-bound EGF. Endosomes were isolated from such cells by the method of subcellular fractionation on Percoll density gradient. Addition to isolated endosomes of anti-P-Tyr complexes with immunogold resulted in a significant shift of endosome peak to the high density region. This fact indicates that anti-P-Tyr interacts with phosphotyrosine residues of EGF receptors localized in endosomes.
authors.forename: A M
authors.initials: AM
authors.lastname: Nesterov
authors.forename: I B
authors.initials: IB
authors.lastname: Vdovina
authors.forename: E S
authors.initials: ES
authors.lastname: Kornilova
authors.forename: N N
authors.initials: NN
authors.lastname: Nikol'skiĭ
mesh_terms: D000906:Antibodies / Q000302:isolation & purification; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000378:metabolism*; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D004815:Epidermal Growth Factor; D066246:ErbB Receptors / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D015388:Organelles / Q000276:immunology / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000276:immunology / Q000378:metabolism*
chemical_list: D000906:Antibodies; D019000:Phosphotyrosine; D014443:Tyrosine; D004815:Epidermal Growth Factor; D066246:ErbB Receptors
vernacular_title: Priamoe dokazatel'stvo fosforilirovaniia po tirozinu retseptorov épidermal'nogo faktora rosta, internalizovannykh v éndosomakh kletok A431.