Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using colloidal silver.
A rapid and simple assay for quantification of proteins in sodium dodecyl sulfate-sample buffer is described. Proteins are bound to a nitrocellulose membrane, stained with colloidal silver, and quantified by transmission densitometry. The staining requires 1 microL of protein sample and takes less than 20 min. Good linearity between the staining intensity and amount of proteins is in the range of 5-100 ng.
Dráber
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Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
D002021:Buffers; D003101:Collodion; D003102:Colloids; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008567:Membranes, Artificial; D011506:Proteins / Q000737:chemistry*; D012835:Silver Nitrate; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling / Q000379:methods
D002021:Buffers; D003102:Colloids; D008567:Membranes, Artificial; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D003101:Collodion; D012835:Silver Nitrate