title: Studies on glycoprotein 13 (gp13) of equid herpesvirus 1 using affinity-purified gp13, glycoprotein-specific monoclonal antibodies and synthetic peptides in a hamster model.
abstract: Hamsters were immunized with either an affinity-purified preparation of equid herpesvirus 1 (EHV-1) glycoprotein 13 (gp13) or synthetic peptides representing three sequences within the homologous glycoprotein of EHV-4, resulting in the production of anti-peptide (in the case of peptide-immunized animals) or antivirus antibodies. The sera from gp13-immunized hamsters contained antibodies which showed virus-neutralizing activity and complement-mediated antibody lysis of EHV-1-infected target cells. These hamsters were protected from EHV-1 challenge. The characteristics of a panel of anti-gp13 monoclonal antibodies (P28, P17, 14H7, 16E4 and 16H9) were assessed both in vivo and in vitro. 16E4 and P28 showed high levels of complement-mediated neutralization of virus, complement-mediated lysis of virus-infected target cells and passive protection of hamsters. Furthermore, epitope mapping studies demonstrated that this glycoprotein contains a neutralizing epitope recognized by EHV-1-immune horse serum. The data imply that gp13 has potential as a candidate antigen for a molecular vaccine.
authors.affiliation: AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, U.K.
authors.forename: A
authors.initials: A
authors.lastname: Stokes
authors.forename: A H
authors.initials: AH
authors.lastname: Corteyn
authors.forename: L A
authors.initials: LA
authors.lastname: Pullen
authors.forename: T R
authors.initials: TR
authors.lastname: Doel
authors.forename: D M
authors.initials: DM
authors.lastname: Meredith
authors.forename: R A
authors.initials: RA
authors.lastname: Killington
authors.forename: I W
authors.initials: IW
authors.lastname: Halliburton
authors.forename: G R
authors.initials: GR
authors.lastname: Whittaker
authors.forename: L A
authors.initials: LA
authors.lastname: Wheldon
authors.forename: L
authors.initials: L
authors.lastname: Nicolson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D002846:Chromatography, Affinity; D003165:Complement System Proteins; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D004861:Herpesvirus 1, Equid / Q000276:immunology* / Q000302:isolation & purification; D007116:Immunization, Passive; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008647:Mesocricetus; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010455:Peptides / Q000138:chemical synthesis; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D010455:Peptides; D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; D003165:Complement System Proteins