title: (K15R M52E) aprotinin is a weak Kunitz-type inhibitor of HIV-1 replication in H9 cells.
abstract: The (K15R M52E) aprotinin is a recombinant molecule with a broader inhibition spectrum against serine proteinases and a higher affinity towards certain proteinases as compared to native aprotinin. This aprotinin variant was produced in E. coli, isolated and purified to homogeneity. The inhibitor was further tested for its effectiveness to reduce human immunodeficiency virus type 1 (HIV-1) replication. Virus growth was followed by an ELISA which detects the amount of virus core protein p24. At a concentration of 50 microM, the recombinant (K15R M52E) aprotinin clearly reduced HIV-1 replication in H9 cells.
authors.affiliation: Abteilung für Klinische Chemie, Universität München, FRG.
authors.forename: E A
authors.initials: EA
authors.lastname: Auerswald
authors.forename: A
authors.initials: A
authors.lastname: Schubert
authors.forename: M
authors.initials: M
authors.lastname: Dolinar
authors.forename: L
authors.initials: L
authors.lastname: Gürtler
authors.forename: F
authors.initials: F
authors.lastname: Deinhardt
mesh_terms: D000595:Amino Acid Sequence; D007611:Aprotinin / Q000235:genetics / Q000494:pharmacology*; D002460:Cell Line; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D014361:Trypsin Inhibitors / Q000235:genetics / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D016655:HIV Core Protein p24; D015699:HIV Envelope Protein gp120; D014361:Trypsin Inhibitors; D007611:Aprotinin