Antiviral activity of sulfated polysaccharides against African swine fever virus.
The polyanionic substances lambda and kappa carrageenan, pentosan polysulfate, fucoidan, dextran sulfate and heparin were investigated for their inhibitory effect on the replication of African swine fever virus (ASFV) in vitro. Lambda carrageenan was the most efficacious with a selectivity index, as based on the ratio of the 50% cytotoxic concentration to the 50% antiviral effective concentration, of 120, followed by pentosan polysulfate with 30, kappa carrageenan 13.3 and fucoidan 10. Dextran sulfate and heparin were almost inactive. In general, the substances had low toxicity for Vero cells. The studies with radiolabeled ASF virions suggest that the sulfated polysaccharides inhibit virus adsorption. Inhibition of virus replication was found for all the polysaccharides only when the substances were present during virus adsorption, with the exception of lambda and kappa carrageenan, which were also inhibitory when added immediately after the adsorption period.
García-Villalón
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D
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientificas, Madrid, Spain.
Gil-Fernández
C
C
D000327:Adsorption / Q000187:drug effects; D000358:African Swine Fever Virus / Q000187:drug effects*; D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D016264:Dextran Sulfate / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D010426:Pentosan Sulfuric Polyester / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D014709:Vero Cells; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects*
D011134:Polysaccharides; D010426:Pentosan Sulfuric Polyester; D002351:Carrageenan; D006493:Heparin; D016264:Dextran Sulfate; C007789:fucoidan