Large scale purification and immunological characterization of human placental nerve growth factor.
Nerve growth factor (NGF) is a protein which plays a critical role in the development and survival of not only peripheral neurons, but possibly also cholinergic brain neurons. The present study describes a procedure for large scale isolation of human NGF of placental origin, and its immunological characterization. A protein species of approximately 26 kDa was obtained, which cross-reacted with antibodies to mouse NGF. Polyclonal and monoclonal anti-mouse NGF antibodies appeared to recognize different bands within this human NGF preparation. Although these polyclonal antibodies recognized both the dimeric and monomeric forms of mouse NGF, the monoclonal antibody recognized only a band corresponding to the dimeric form of mouse NGF.
Bigon
E
E
Advanced Technology Division, Fidia Research Laboratories, Abano Terme, Italy.
Soranzo
C
C
Minozzi
C
C
Skaper
S D
SD
Callegaro
L
L
D000906:Antibodies; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003429:Cross Reactions; D003956:Dialysis / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D000939:Epitopes / Q000032:analysis; D005260:Female; D005612:Freeze Drying; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000276:immunology / Q000302:isolation & purification*; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D014462:Ultrafiltration / Q000379:methods
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes; D046911:Macromolecular Substances; D009414:Nerve Growth Factors