Gas chromatographic assay for N,N-dimethylglycine in urine.
A gas chromatographic method for the determination of N,N-dimethylglycine in urine has been developed. After clean-up by cation-exchange, N,N-dimethylglycine was derivatized with ethanol and hydrochloric acid to form the corresponding ethyl ester. After evaporation of solvent, N,N-dimethylglycine ethyl ester was extracted into methylene chloride and chromatographed on a gas chromatograph equipped with a packed column containing 10% Carbowax 20 M. The detection limit of the method is 0.01 mM N,N-dimethylglycine in urine. This method has been used to detect N,N-dimethylglycine in urine from healthy subjects as well as in urine from patients with metabolic disorders. These findings were verified by gas chromatography-mass spectrometry.
Tjørnelund
J
J
Department of Organic Chemistry, Royal Danish School of Pharmacy, Copenhagen.
Hansen
S H
SH
D001622:Betaine / Q000627:therapeutic use; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006712:Homocystinuria / Q000188:drug therapy / Q000652:urine; D006801:Humans; D006943:Hyperglycemia / Q000652:urine; D007202:Indicators and Reagents; D009153:Mutagens / Q000737:chemistry / Q000652:urine*; D012521:Sarcosine / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000652:urine; D013696:Temperature
D007202:Indicators and Reagents; D009153:Mutagens; D001622:Betaine; C025138:dimethylglycine; D012521:Sarcosine