Expression of MHC class II antigens on human glioma cells modulated by transfection with genes encoding these antigens.
Four glioma cell lines not expressing human leukocyte antigen (HLA)-DR or DQ did so after transfection with genes encoding HLA-DR and DQ. The glioma cells had enhanced ability to stimulate allogeneic and autologous responding lymphocytes in the mixed lymphocyte response (MLR). Glioma cells expressing only HLA-DR had weaker MLR enhancement than those expressing both HLA-DR and DQ or only HLA-DQ. Stimulation by transformed glioma cells increased the killing activity of responding lymphocytes against autologous glioma cells 2.0 times. Both HLA-DR and DQ are important in MLR, and the immunogenicity of glioma cells might be increased by transfection with genes encoding these antigens.
Yoshida
S
S
Department of Neurosurgery, School of Medicine, Niigata University.
Takahashi
H
H
Tanaka
R
R
D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D001932:Brain Neoplasms / Q000276:immunology / Q000473:pathology*; D003601:Cytotoxicity Tests, Immunologic; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000276:immunology / Q000473:pathology*; D006683:HLA-DQ Antigens / Q000096:biosynthesis* / Q000235:genetics; D006684:HLA-DR Antigens / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000276:immunology
D000951:Antigens, Neoplasm; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D011994:Recombinant Proteins