title: Liposomal malaria vaccine in humans: a safe and potent adjuvant strategy.
abstract: This study describes the safety and immunogenicity of a liposome-based vaccine injected into human subjects. Thirty healthy adult male volunteers were immunized with a liposome-encapsulated recombinant protein (R32NS181) containing epitopes from the repeat region of the circumsporozoite protein of Plasmodium falciparum. This antigen had previously been found to be poorly immunogenic in humans when it was adsorbed with Al(OH)3. In the present study, R32NS181 was encapsulated in liposomes containing monophosphoryl lipid A that were subsequently adsorbed to Al(OH)3. Increasing doses of liposomes containing antigen and monophosphoryl lipid A were used, but the liposomes were always adsorbed to the same dose of Al(OH)3. R32-specific serum IgG antibody responses to liposome-encapsulated R32NS181 were much higher than levels attained previously in humans with R32NS181 adsorbed to Al(OH)3. Geometric mean specific IgG levels after three doses ranged from 14 to 33 micrograms/ml. Sera from volunteers receiving the two highest doses inhibited P. falciparum sporozoite invasion of cultured hepatoma cells by an average of 92%, a result that was again superior to previously reported vaccines. Moderate but acceptable transient local reactogenicity was noted at high doses of the vaccine formulation, but little or no systemic toxicity was seen despite liposomal monophosphoryl lipid A doses up to 2200 micrograms. We conclude that encapsulation of poorly immunogenic circumsporozoite protein repeat peptides in monophosphoryl lipid A-containing liposomes is a successful adjuvant strategy in humans for inducing high levels of specific antibody production.
authors.affiliation: Center for Immunization Research, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, MD 21205.
authors.forename: L F
authors.initials: LF
authors.lastname: Fries
authors.forename: D M
authors.initials: DM
authors.lastname: Gordon
authors.forename: R L
authors.initials: RL
authors.lastname: Richards
authors.forename: J E
authors.initials: JE
authors.lastname: Egan
authors.forename: M R
authors.initials: MR
authors.lastname: Hollingdale
authors.forename: M
authors.initials: M
authors.lastname: Gross
authors.forename: C
authors.initials: C
authors.lastname: Silverman
authors.forename: C R
authors.initials: CR
authors.lastname: Alving
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000328:Adult; D000595:Amino Acid Sequence; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D002478:Cells, Cultured / Q000469:parasitology; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D066298:In Vitro Techniques; D008050:Lipid A / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000276:immunology; D008081:Liposomes; D008288:Malaria / Q000517:prevention & control*; D008297:Male; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000009:adverse effects / Q000737:chemistry
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D008050:Lipid A; D008081:Liposomes; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C048436:monophosphoryl lipid A