title: Hepatocellular expression of lymphocyte function-associated antigen 3 in chronic hepatitis.
abstract: T lymphocyte-mediated cytolytic immune reactions are considered a major cause of hepatocyte injury in chronic viral and autoimmune hepatitis. To further investigate local immune responses, we studied the expression of lymphocyte antigens and cell-cell interaction molecules known to be involved in effector-target cell interactions by light and electron microscopy in liver biopsy specimens from patients with chronic viral and autoimmune hepatitis. CD8+ lymphocytes were found to be the predominant population of cells in the inflammatory infiltrate in chronic hepatitis B and non-A, non-B hepatitis. In contrast, CD4+ cells constituted a comparably higher proportion of cells and were more numerous than CD8+ cells in chronic autoimmune hepatitis. In both viral and autoimmune hepatitis, a substantial portion of lymphocytes expressed activation antigens such as T11/3 (CD2R) and IL-2-R (CD25). Lymphocyte function-associated antigen-3 (CD58), which mediates lymphocyte adhesion and activation and is the natural ligand of the CD2/T11 lymphocyte surface receptor, could be demonstrated on endothelial cells and hepatocytes. Hepatocellular lymphocyte function-associated antigen-3 expression in chronic hepatitis showed membranous and cytoplasmic staining of hepatocytes and had a positive correlation with the degree of inflammatory activity. These results suggest that effector-target interactions between hepatocytes and lymphocytes mediated by the lymphocyte function-associated antigen-3/CD2 pathway play a role in chronic inflammatory liver disease. Possible functional consequences of this interaction include enhancement of antigen-specific immune reactions and antigen-independent mechanisms of T cell activation, which may contribute considerably to the degree of inflammatory activity and tissue damage in chronic hepatitis.
authors.affiliation: Institut für Pathologie, Johannes Gutenberg Universität, Mainz, Federal Republic of Germany.
authors.forename: F
authors.initials: F
authors.lastname: Autschbach
authors.forename: S C
authors.initials: SC
authors.lastname: Meuer
authors.forename: U
authors.initials: U
authors.lastname: Moebius
authors.forename: M
authors.initials: M
authors.lastname: Manns
authors.forename: G
authors.initials: G
authors.lastname: Hess
authors.forename: K H
authors.initials: KH
authors.lastname: Meyer zum Büschenfelde
authors.forename: W
authors.initials: W
authors.lastname: Thoenes
authors.forename: H P
authors.initials: HP
authors.lastname: Dienes
mesh_terms: D000954:Antigens, Surface / Q000276:immunology*; D015415:Biomarkers; D018968:CD58 Antigens; D002908:Chronic Disease; D006680:HLA Antigens / Q000032:analysis; D006505:Hepatitis / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000276:immunology*; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets / Q000473:pathology; D008562:Membrane Glycoproteins / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D018968:CD58 Antigens; D006680:HLA Antigens; D008562:Membrane Glycoproteins