[The determination of antigen-specific immune complexes by an immunoenzyme method].
The methodological approach permitting the detection of immune complexes containing specific antibodies to a definite antigen in the enzyme-linked immunosorbent assay (ELISA) is described. The basic conditions of the assay were optimized. Immune complexes were precipitated from blood serum with 3.5% polyethylene glycol 6000 for 4 hours. The precipitate thus obtained was dissolved and incubated in polystyrene plates with immobilized antigen at 37 degrees C for a long time (at least 6 hours) in a humid chamber. The amount of bound antibodies, determined by ELISA techniques, was conjectured from the level of antigen-specific immune complexes. The proposed approach can be used in the immunodiagnosis of infectious diseases.
Bobrovnik
S A
SA
Liashchenko
K P
KP
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000302:isolation & purification; D000936:Antigen-Antibody Complex / Q000097:blood*; D001500:BCG Vaccine / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000097:blood*; D005260:Female; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D009169:Mycobacterium tuberculosis / Q000276:immunology; D014373:Tuberculin / Q000276:immunology*
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D001500:BCG Vaccine; D000939:Epitopes; D007106:Immune Sera; D014373:Tuberculin
Opredelenie antigenspetsifichnykh immunnykh kompleksov immunofermentnym metodom.