title: Amyloid-like properties of a synthetic peptide corresponding to the carboxy terminus of beta-amyloid protein precursor.
abstract: A synthetic peptide whose sequence corresponds to the 20 carboxy-terminal amino acids of beta-amyloid protein precursor (APP) was found to form fibrils in vitro. These fibrils showed birefringence in polarized light when stained with Congo red, fluoresced when bound with thioflavin S, were resistant to proteases, and had a cross-beta conformation. By contrast, peptides with other sequences from the intracellular domain of APP and a peptide corresponding to this entire domain did not exhibit the full range of beta-amyloid properties. These results suggest that a fragment from the C-terminus of the beta-amyloid protein precursor could bind to intraneuronal paired helical filaments and account for some of its amyloid-like properties.
authors.affiliation: ICI Pharmaceuticals Group, Wilmington, Delaware 19897.
authors.forename: C B
authors.initials: CB
authors.lastname: Caputo
authors.forename: P E
authors.initials: PE
authors.lastname: Fraser
authors.forename: I E
authors.initials: IE
authors.lastname: Sobel
authors.forename: D A
authors.initials: DA
authors.lastname: Kirschner
mesh_terms: D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry* / Q000648:ultrastructure; D000818:Animals; D010450:Endopeptidases; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D000685:Serum Amyloid A Protein / Q000737:chemistry / Q000648:ultrastructure; D013329:Structure-Activity Relationship; D013552:Swine; D014961:X-Ray Diffraction
chemical_list: D016564:Amyloid beta-Protein Precursor; D010455:Peptides; D000685:Serum Amyloid A Protein; D010450:Endopeptidases