The effect of lentinan on proliferative processes in parenchymal organs of rats--I. The effect on pyrimidine and nucleic acid syntheses.
The present study investigated the effect of Lentinan on the biochemical events associated with the pyrimidine and nucleic acid syntheses in the liver, kidney, thymus and spleen of rats. Lentinan was used at a dose of 4 mg/kg/day (twice) and in a single dose of 20 mg/kg. The following changes were observed. (1) The utilization of (14C)orotic acid for the synthesis of uridine components of liver acid-soluble extract and RNA uracil was activated after the administration of both doses of the drug. The specific activity of cytidine components of the acid-soluble extract and RNA were, on the other hand, not affected. The same holds true for the kidney. The ratio of the specific activity of cytidine:uridine components of the acid soluble extract as well as RNA decreased after the administration of both doses of the drug. The specific activity of DNA cytosine and thymine are slightly suppressed in the liver after the administration of a high dose of Lentinan; no effect was observed in the kidney. (2) The uptake of (14C)cytidine by the liver was not affected; the specific activity of DNA cytosine and thymine were increased after the administration of a high dose of Lentinan. (3) The uptake of (14C)thymidine by the liver was not affected; the specific activity of liver DNA thymine was increased after the administration of both doses of the drug. In the thymus an increase of specific activity of DNA thymine has also been observed. (4) Repeated doses of the drug (4 mg/kg for 6 consecutive days) increased the weight of the spleen. The specific activity of DNA thymine of the liver and spleen were significantly increased.
Seifert
J
J
Institute of Pharmacology, Czechoslovak Academy of Sciences, Prague.
Mostecká
H
H
Machková
Z
Z
Masek
K
K
Kaneko
Y
Y
Chihara
G
G
D000818:Animals; D003562:Cytidine / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007912:Lentinan / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009963:Orotic Acid / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism
D011743:Pyrimidines; D007912:Lentinan; D003562:Cytidine; D009963:Orotic Acid; D012313:RNA; D004247:DNA; D013936:Thymidine