Atrial peptides induce mast cell histamine release.
Human atrial natriuretic peptide [ANF(1-28)] contains five arginine residues and carries an overall positive change of four. It was hypothesized that atrial peptides may induce mast cell histamine release. In vitro, three atrial peptides [ANF(1-28), (3-28) and (5-28)] were demonstrated to induce dose-dependent histamine release from isolated rat peritoneal mast cells. In vivo, ANF(3-28) produced a dose-dependent increase in rat skin permeability which was blocked by antagonists of histamine and serotonin. The results indicate atrial peptides are capable of inducing mast cell degranulation in a manner similar to that described for other positively charged peptides.
Opgenorth
T J
TJ
Abbott Laboratories, Abbott Park, IL 60064.
Budzik
G P
GP
Mollison
K W
KW
Davidsen
S K
SK
Holst
M R
MR
Holleman
W H
WH
D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006633:Histamine Antagonists / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D007271:Injections, Intradermal; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012702:Serotonin Antagonists / Q000494:pharmacology; D013268:Stimulation, Chemical; D013329:Structure-Activity Relationship; D013373:Substance P / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D006633:Histamine Antagonists; D010446:Peptide Fragments; D012702:Serotonin Antagonists; C049490:atrial natriuretic factor (3-28); D013373:Substance P; D003189:p-Methoxy-N-methylphenethylamine; D009320:Atrial Natriuretic Factor; C054538:atrial natriuretic factor (5-28)