[Virus demonstration and pathologic changes in different phases of coxsackievirus B myocarditis in mice].
A/J mice between 15 days and 10 weeks of age were infected intraperitoneally with Coxsackievirus B3 (CVB3). To search for virus in the myocardium various methods were applied: virus isolation from the myocardium, RNA extraction for dot blot hybridization and in situ hybridization. Two different RNA probes, one specific for CVB3 the other cross-reacting with other enteroviruses, were radioactively labeled with 35S or 32P by in vitro transcription. In paraffin sections histological alterations were assessed semiquantitatively. The animals developed acute myocarditis with myolysis and virus in the myocardium until 14 days after infection. The second stage of the disease was characterized by a persistent inflammatory infiltrate. At this stage no virus could be shown in the myocardium. Antibodies against cardiac myosin appeared 16 days after infection. Autoimmune mechanisms thus seem to be a most relevant factor for persistent inflammation after the acute viral phase of the disease.
Rabausch-Starz
I
I
Pathologisches Institut, Universität Würzburg.
Neu
N
N
Müller-Hermelink
H K
HK
D000818:Animals; D003384:Coxsackievirus Infections / Q000175:diagnosis / Q000473:pathology*; D029822:Enterovirus B, Human / Q000235:genetics / Q000302:isolation & purification*; D006321:Heart / Q000382:microbiology*; D051379:Mice; D008805:Mice, Inbred A; D009205:Myocarditis / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification
D012367:RNA, Viral
Virusnachweis und pathologische Veränderungen in verschiedenen Phasen der Coxsackievirus B Myokarditis bei Mäusen.