Effects of lindane on fluidity and lipid composition in rat renal cortex membranes.
The influence of lindane upon dynamic properties of plasma membranes from rat renal cortex has been investigated using a fluorescence polarization technique. Preincubation with lindane increased membrane fluidity in a manner that is dose-dependent. This increase was higher in brush border membranes than in basolateral membranes. However, a significant decrease of the membrane fluidity was found in brush border membranes when rats were injected with lindane for 12 days. A possible solution to this difference could involve a resistance to membrane disordering by lindane through a regulatory mechanism that would balance the amount of cholesterol and phospholipid classes in the renal cortex membranes of lindane-injected rats.
Pérez-Albarsanz
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Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Alcalá de Henares-Madrid, Spain.
López-Aparicio
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P
Senar
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Recio
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D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D002784:Cholesterol / Q000032:analysis; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005454:Fluorescence Polarization; D001556:Hexachlorocyclohexane / Q000494:pharmacology*; D007672:Kidney Cortex / Q000187:drug effects* / Q000648:ultrastructure; D007700:Kinetics; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D008563:Membrane Lipids / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D008563:Membrane Lipids; D010743:Phospholipids; D001556:Hexachlorocyclohexane; D002784:Cholesterol; D000469:Alkaline Phosphatase; D000254:Sodium-Potassium-Exchanging ATPase; D004121:Dimethyl Sulfoxide