Separation and characterization of sialic acid-containing salivary-type amylase from patients' sera with immunoglobulin A-type myeloma.
Isoamylases, with an abnormal anodic migration, were detected by an electrophoretic technique in the sera from two patients with immunoglobulin A-type myeloma. The abnormal isoamylase bands migrate towards the anode faster than the salivary isoamylase (S2) band and were stained more strongly than the S2 sub-band. The abnormal isoamylase could be separated from patients' sera by using size-exclusion high-performance liquid chromatography. The serum abnormal isoamylases were showed to be sialic acid residues containing amylase, after the study of treatment with neuraminidase (EC 3.2.1.18), and to be salivary-type amylase, after the study of reaction with human salivary monoclonal antibody. The abnormal bands were not detected in the saliva from one patient. The two patients had no detectable malignancies except myeloma. These findings strongly suggest that the sialic acid-containing salivary-type amylases were produced ectopically from myeloma cells. In this regard the ectopic amylase production by myeloma cells is discussed.
Moriyama
T
T
Clinical Laboratory, Asahikawa Medical College, Japan.
Tozawa
T
T
Yamashita
H
H
Onodera
S
S
Nobuoka
M
M
Makino
M
M
D000368:Aged; D000369:Aged, 80 and over; D000681:Amylases / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D008297:Male; D009101:Multiple Myeloma / Q000097:blood* / Q000276:immunology; D019158:N-Acetylneuraminic Acid; D012463:Saliva / Q000201:enzymology; D012794:Sialic Acids / Q000032:analysis*
D007070:Immunoglobulin A; D012794:Sialic Acids; D000681:Amylases; D019158:N-Acetylneuraminic Acid