title: Bacteria-induced histamine release from human bronchoalveolar cells and blood leukocytes.
abstract: Histamine release induced by Staphylococcus aureus was examined in cells obtained by bronchoalveolar lavage (BAL) in non-atopic individuals. Approximately half of the individuals responded with mediator release to the bacterium, and the release was found to be time- and concentration dependent. No difference was found between the patients who responded and those who did not respond in regard to age, sex, smoker/non-smoker, % recovery of BAL-fluid, total cell count, differential cell counts, histamine content per mast cell, or diagnoses. Also stimulation of the BAL-cells with the calcium-ionophore A23187 resulted in histamine release. S. aureus-induced histamine release from basophils was examined in leukocyte suspensions obtained from the same individuals, and in all experiments release was found. The dose-response curves were similar to those obtained with BAL cells. The bacteria-induced mediator release from superficially lying cells in the airways epithelium might be of importance for the precipitation or exacerbation of bronchial asthma in respiratory tract infections.
authors.affiliation: Department of Pharmacology, University of Copenhagen, Denmark.
authors.forename: P
authors.initials: P
authors.lastname: Clementsen
authors.forename: N
authors.initials: N
authors.lastname: Milman
authors.forename: E
authors.initials: E
authors.lastname: Struve-Christensen
authors.forename: B N
authors.initials: BN
authors.lastname: Petersen
authors.forename: M
authors.initials: M
authors.lastname: Pedersen
authors.forename: H
authors.initials: H
authors.lastname: Bisgaard
authors.forename: H
authors.initials: H
authors.lastname: Permin
authors.forename: S
authors.initials: S
authors.lastname: Norn
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000276:immunology / Q000382:microbiology; D001491:Basophils / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology* / Q000378:metabolism; D002452:Cell Count; D005260:Female; D006636:Histamine Release; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008407:Mast Cells; D008875:Middle Aged; D013203:Staphylococcal Infections / Q000276:immunology; D013211:Staphylococcus aureus / Q000502:physiology*; D013997:Time Factors