Acridine orange stain for determining intracellular enteropathogens in HeLa cells.
Green-fluorescent intracellular enteropathogenic bacteria were observed after infected HeLa cell monolayers were stained with acridine orange and counterstained with crystal violet at least 3 h after infection.
Miliotis
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MD
Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
D000165:Acridine Orange; D004755:Enterobacteriaceae / Q000648:ultrastructure*; D004756:Enterobacteriaceae Infections / Q000175:diagnosis*; D005456:Fluorescent Dyes; D005840:Gentian Violet; D006367:HeLa Cells / Q000382:microbiology*; D006801:Humans; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D000165:Acridine Orange; D005840:Gentian Violet