The Na+/H+ exchanger role in the intracellular pH regulation of human platelets.
The intracellular pH of human platelets was measured with a fluorescent intracellular probe. When platelets were in basal conditions (pHo 7.4, [Na+]o 140 mM) the pHi was 6.98 +/- 0.04. Five minutes after the addition of EIPA 60 microM, an inhibitor of Na+/H+ exchange, the pHi fell in 0.075 +/- 0.022 pH units (P less than 0.05). Preincubation in a sodium free, acid medium (pHo 6.3) induced a cell acidification to pH 6.61 +/- 0.03 (P less than 0.01). Preacidified platelets showed a recovery in sodium-containing solution that is a function of [Na+]o. The initial rate of recovery depends on [Na+]o in a Michaelis-Menten fashion, showing a Km of 35.6 mM and a Vmax of 0.213 pH units/min. These results show that the pHi is maintained in human platelets by a Na+/H+ exchange that is active even in basal conditions. The properties of the Na+/H+ exchanger in human platelets and in the less accessible smooth vascular cells are similar; generalized pathological alterations, like hypertension, could be reflected by both tissues.
Gende
O A
OA
Centro de Investigaciones Cardiovasculares, Facultad de Ciencias Médicas Universidad Nacional de La Plata, Argentina.
Cingolani
H E
HE
D000136:Acid-Base Equilibrium / Q000502:physiology*; D000584:Amiloride / Q000031:analogs & derivatives / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002352:Carrier Proteins / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012964:Sodium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers
D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride; D012964:Sodium; C039614:ethylisopropylamiloride