Effect of subarachnoid hemorrhage on serotonin uptake and metabolism in rabbit basilar artery.
The effects of subarachnoid hemorrhage (SAH) on neuronal uptake and metabolism of serotonin (5-HT) in the rabbit basilar artery were examined. Extracted 3H-amines from the isolated arteries after incubation with [3H]5-HT were separated by column chromatography. Radioactivity of 5-HT and 5-hydroxyindoleacetic acid was, respectively, 52.7 +/- 13.9 and 22.9 +/- 5.4 x 10(2) dpm/mg tissue in the control group (n = 8); 32 and 18% of control after denervation (n = 6); 99 and 12% of control after treatment with pargyline (n = 7); and 65 and 76% of control after SAH (n = 7). These results suggest that the neuronal uptake of 5-HT is impaired by SAH, although monoamine oxidase activity is relatively preserved.
Tanaka
Y
Y
Department of Neurological Surgery, University of Virginia Health Sciences Center, Charlottesville 22908.
Kassell
N F
NF
Machi
T
T
Dougherty
D A
DA
D000818:Animals; D001345:Autoradiography; D001488:Basilar Artery / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D003714:Denervation; D006892:Hydroxydopamines / Q000494:pharmacology; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000378:metabolism; D016627:Oxidopamine; D010293:Pargyline / Q000494:pharmacology; D011817:Rabbits; D012701:Serotonin / Q000378:metabolism*; D013345:Subarachnoid Hemorrhage / Q000378:metabolism*
D006892:Hydroxydopamines; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D016627:Oxidopamine; D010293:Pargyline