title: [A stable reagent for the-single stage determination of inorganic phosphate].
abstract: A recipe of a simple reagent for phosphorus detection has been developed, consisting of ammonium molybdate (4 mM), sulfuric acid (0.2 N), and Tween-80 (0.2%). The developing phosphate staining may be registered in 15 min at a wavelength of 350 nm. The product molar extinction is equal to 1.20.10(4) M-1.cm-1, this being close to that of molybdic blue. Phosphate staining is characterized by the stability of results and insensitivity to the presence of a number of substances used in enzymology. The prepared reagent is fit for experiments within a fortnight if stored in the cold.
authors.forename: A B
authors.initials: AB
authors.lastname: Pupyshev
mesh_terms: D007202:Indicators and Reagents; D010758:Phosphorus / Q000032:analysis*; D013194:Staining and Labeling
chemical_list: D007202:Indicators and Reagents; D010758:Phosphorus
vernacular_title: Stabil'nyĭ reaktiv dlia odnostadiĭnogo opredeleniia neorganicheskogo fosfata.