title: Physiological effects of the fructose-1,6-diphosphate aldolase ts8 mutation on stable RNA synthesis in Escherichia coli.
abstract: The conditional lethal mutations ts8 and h8 are located in fda, the gene encoding aldolase, and they inhibit RNA synthesis upon shift to the nonpermissive temperature. We demonstrate that both mutations preferentially inhibit stable RNA synthesis and that this inhibition occurs at the level of transcription initiation. The susceptibility of a promoter to the inhibitory effects of ts8 is correlated with the ability of the promoter to be growth rate regulated. This effect is independent of relA and spoT function. Inhibition is dependent upon glucose metabolism past the generation of glucose-6-phosphate; however, the mechanism of this effect is unknown.
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison 53706.
authors.forename: M
authors.initials: M
authors.lastname: Singer
authors.forename: W A
authors.initials: WA
authors.lastname: Walter
authors.forename: B M
authors.initials: BM
authors.lastname: Cali
authors.forename: P
authors.initials: P
authors.lastname: Rouviere
authors.forename: H H
authors.initials: HH
authors.lastname: Liebke
authors.forename: R L
authors.initials: RL
authors.lastname: Gourse
authors.forename: C A
authors.initials: CA
authors.lastname: Gross
mesh_terms: D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D012329:RNA, Bacterial; D012333:RNA, Messenger; D005634:Fructose-Bisphosphate Aldolase; D005947:Glucose