title: Novel antipeptide antibodies to the human glucocorticoid receptor: recognition of multiple receptor forms in vitro and distinct localization of cytoplasmic and nuclear receptors.
abstract: We have synthesized two peptides that correspond to unique regions of the amino-terminus of the human glucocorticoid receptor (GR). Peptides representing amino acids 245-259 and 346-367 (designated 59 and 57, respectively) were chosen on the basis of hydrophobicity/hydrophilicity ratios as well as overall proline content. These peptides were then used as antigens to produce epitope-specific antibodies that recognize and interact with human GR in a variety of physical states. Antiserum directed against each peptide recognizes denatured, [3H] dexamethasone mesylate-labeled GR as well as unliganded receptor on Western blots. In contrast to other antipeptide GR antibodies, these antibodies recognize and form stable complexes with unactivated and molybdate-stabilized forms of the GR, indicating that neither epitope is occluded when the receptor exists in an oligomeric state. Activated, 4S DNA-binding forms of the receptor are also recognized by both antibodies. The interaction of antibodies 59 and 57 with human GR in various states is highly specific based on the observation that preincubation of either antiserum with the appropriate peptide completely precludes the recognition of receptor by antibody. Titration analysis of antisera reveals that an increase in the antibody concentration cause discrete increases in the sedimentation coefficient of GR on sucrose gradients. These shifts occur under high salt conditions and are consistent with the formation of multiple stable antibody-receptor complexes. Interestingly, neither antibody interferes with the ability of the GR to be activated into a DNA-binding form or with the ability of the activated GR to interact with DNA cellulose. Consistent with these observations, both antibodies recognize and form stable complexes with GR when the receptor is associated with DNA fragments that contain specific glucocorticoid-responsive elements. Thus, both antibodies appear to recognize all known forms of the human GR protein. Using immunohistochemical techniques to visualize GR in HeLa S3 cells as well as in Chinese hamster ovary cells that stably express transfected human GR, a cytoplasmic location for receptor is observed in the absence of ligand. In contrast, immunoreactive GR is predominantly nuclear after hormone treatment, further supporting a role for nuclear translocation in GR function.
authors.affiliation: Department of Physiology, University of North Carolina, Chapel Hill 27599.
authors.forename: J A
authors.initials: JA
authors.lastname: Cidlowski
authors.forename: D L
authors.initials: DL
authors.lastname: Bellingham
authors.forename: F E
authors.initials: FE
authors.lastname: Powell-Oliver
authors.forename: D B
authors.initials: DB
authors.lastname: Lubahn
authors.forename: M
authors.initials: M
authors.lastname: Sar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D015153:Blotting, Western; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry*; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003593:Cytoplasm / Q000737:chemistry*; D004247:DNA / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D011489:Protein Denaturation; D011965:Receptors, Glucocorticoid / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D014162:Transfection
chemical_list: D000906:Antibodies; D000941:Antigens; D000939:Epitopes; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D004247:DNA