title: Characterization of methylated bovine serum albumin-induced allergic inflammation in rats.
abstract: An air pouch type allergic inflammation in rats was induced using an insoluble cationic protein, methylated bovine serum albumin (MeBSA), as an antigen. Changes in vascular permeability, local tissue edema, histamine contents in the pouch fluid, and number of infiltrated leukocytes and chemotactic activity in the pouch fluid were analyzed during an 8-hour period after injecting the antigen solution into the air pouch of the immunized and nonimmunized rats. Vascular permeability during the first 30-min interval in the immunized rats was higher than that in the nonimmunized rats, reflecting a higher histamine level in the pouch fluid. However, both the increase in vascular permeability and histamine level in the immunized rats in this period were much lower than those induced by a soluble, noncationic antigen, azobenzenearsonate-conjugated acetyl bovine serum albumin. In the MeBSA-induced allergic inflammation model, a second peak of vascular permeability was induced at 2 h, and local tissue edema formation became apparent at 2 h, reaching a plateau at 4 h. A prominent increase in leukocyte infiltration, especially neutrophils, into the pouch fluid was induced at 4 h in accordance with an increase in chemotactic activity in the pouch fluid. These observations indicate that the acute phase of MeBSA-induced allergic inflammation is characterized by a weak anaphylactic response and a prominent neutrophil infiltration.
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hasegawa
authors.forename: N
authors.initials: N
authors.lastname: Hirasawa
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Arakawa
authors.forename: S
authors.initials: S
authors.lastname: Tsurufuji
authors.forename: K
authors.initials: K
authors.lastname: Ohuchi
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D004487:Edema / Q000276:immunology; D006636:Histamine Release; D006967:Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D007249:Inflammation; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D012871:Skin Diseases / Q000276:immunology; D010132:p-Azobenzenearsonate / Q000008:administration & dosage
chemical_list: C029180:methylated bovine serum albumin; D012710:Serum Albumin, Bovine; D010132:p-Azobenzenearsonate