Abnormalities in CD4+ T lymphocyte subsets in patients with common variable immunodeficiency.
In order to investigate whether deficient immunoglobulin production in common variable immunodeficiency (CVI) patients was related to defective T cells functions, phenotype and proliferative responses to mitogen of peripheral blood mononuclear cells (PBMC) were investigated in 9 patients with CVI. The results were compared to those of 12 age- and sex-matched normal controls. The numbers of CD3+ and CD8+ T cells in the patients were not different from those in the control group, but the numbers of CD4 T cells were decreased (511 +/- 237 vs 844 +/- 247/mm3; P less than 0.01). The decrease in CD4 T cells was due to a dramatic deficiency in the CD4+ CD45RA+ subset, observed as an absolute value of blood lymphocytes (126 +/- 91 vs 384 +/- 142; P less than 0.001) and as a percentage (9.0 +/- 7.1 vs 18.8 +/- 5.0; P less than 0.01). In contrast, the CD4+ CD29+ T cell subset was not different in CVI from those in the control group. Moreover, there was a strong positive correlation between the number of percentages of CD4+ CD45RA+ blood T cells and the proliferative response of PBMC to PHA (respectively, P less than or equal to 0.02 and P = 0.05) and to Con A (P less than or equal to 0.02). The decrease of CD4+ CD45RA+ T cells could reflect an abnormality in the physiological status of T cells and could be of critical importance in the antibody deficiency.
Lebranchu
Y
Y
Department of Immunology, Faculté de Médecine, Tours, France.
Thibault
G
G
Degenne
D
D
Bardos
P
P
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D019012:Integrin beta1; D017493:Leukocyte Common Antigens; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010641:Phenotype
D015703:Antigens, CD; D015704:CD4 Antigens; D006649:Histocompatibility Antigens; D019012:Integrin beta1; D017493:Leukocyte Common Antigens