Epidermal growth factor (EGF) decreased endothelin-2 (ET-2) production in human renal adenocarcinoma cells.
Production of immunoreactive (ir-) endothelin-2 (ET-2) in renal adenocarcinoma cells, ACHN, was reduced by transforming growth factor-beta, basic fibroblast growth factor, transforming growth factor-alpha and, most strikingly, by epidermal growth factor (EGF). These growth factors did not show such inhibitory effects on the secretion of ir-ET-1 in ET-1-producing cells, indicating that the production of ET-2 and ET-1 is regulated differently by the growth factors. EGF specifically reduced the secretion of not only ir-ET-2 but also ir-big ET-2 with only a small decrease in total protein synthesis. Northern blot analysis indicated that EGF controls the ET-2-production at the transcription levels of ET-2 gene.
Tokito
F
F
Tsukuba Research Laboratories, Takeda Chemical Industries Ltd., Ibaraki, Japan.
Suzuki
N
N
Hosoya
M
M
Matsumoto
H
H
Ohkubo
S
S
Fujino
M
M
D000230:Adenocarcinoma; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D016232:Endothelins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007680:Kidney Neoplasms; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger
D016232:Endothelins; D006133:Growth Substances; D012333:RNA, Messenger; D011061:Poly A; D004815:Epidermal Growth Factor; D012313:RNA; D007930:Leucine