Localization of hepatitis C virus (HCV) antigen by immunohistochemistry on fixed-embedded liver tissue.
An immunohistochemical method of staining HCV-related antigens in fixed-embedded liver biopsies is described. Two primary antisera were used: 1) a high titre anti-HCV human IgG separated from an anti-HCV positive serum; and 2) rabbit anti-HCV antibodies. In our experience this method proves to be reproducible and shows a good correlation with serologic results.
Infantolino
D
D
Servizio di Anatomia ed Istologia, Ospedale di Castelfranco Veneto, Torino, Italy.
Bonino
F
F
Zanetti
A R
AR
Lesniewski
R R
RR
Barbazza
R
R
Chiaramonte
M
M
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification*; D001706:Biopsy; D005404:Fixatives; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000008:administration & dosage; D006526:Hepatitis C / Q000276:immunology / Q000473:pathology; D018936:Hepatitis C Antigens; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000276:immunology / Q000473:pathology*; D013194:Staining and Labeling; D016610:Tissue Embedding
D000956:Antigens, Viral; D005404:Fixatives; D006508:Hepatitis Antibodies; D018936:Hepatitis C Antigens