Immunohistochemistry of porcine skin.
The present paper reports immunohistological findings in porcine skin, which were obtained by use of mono- and polyclonal antihuman antibodies and either alkaline phosphatase anti-alkaline phosphatase (APAAP) or peroxidase (POX) technique. Epidermal staining was observed with antibodies to keratins (K 8.12, RSKE 60), filaggrin, and calmodulin (ACAM). Staining of connective tissue and vessels was achieved using antibodies to vimentin (V9(1)), collagen type IV, and fibronectin. In general, these antibodies gave a staining pattern similar to that of normal human skin. The similarities of immunoreactivity to poly- and monoclonal antihuman antibodies in porcine and human skin render porcine skin a reliable model in biomedical research.
Wollina
U
U
Department of Dermatology, University of Köln, FRG.
Berger
U
U
Mahrle
G
G
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002147:Calmodulin / Q000032:analysis; D000078404:Epidermal Cells; D005347:Fibroblasts / Q000166:cytology; D005353:Fibronectins / Q000032:analysis; D000091344:Filaggrin Proteins; D007124:Immunoenzyme Techniques; D007381:Intermediate Filament Proteins / Q000032:analysis; D007633:Keratins / Q000032:analysis; D012867:Skin / Q000166:cytology*; D013552:Swine; D014746:Vimentin / Q000032:analysis
D000906:Antibodies; D000911:Antibodies, Monoclonal; D002147:Calmodulin; C000717249:FLG protein, human; D005353:Fibronectins; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D014746:Vimentin; D007633:Keratins