Phosphorylation sites of the nicotinic acetylcholine receptor. A novel site detected in position delta S362.
The delta-subunit of the nicotinic acetylcholine receptor from Torpedo californica electric tissue isolated form receptor purified in the absence of protein phosphatase inhibitors contains a total of four phosphate groups. Three of these are shown to represent phosphoserine groups. The fourth possible represents phosphotyrosine. The phosphate groups are localized within the primary structure: We found phosphoserine in positions delta S361 and delta S377, the predicted sites phosphorylated by PKA and PKC, respectively. In addition, we found that position delta S362 is also phosphorylated. Phosphorylation experiments with the synthetic peptide delta L357-delta K368 show that phosphorylation of this novel site can be catalyzed by PKA and by PKC. It is concluded that the delat-subunit of the acetylcholine receptor is stably and not transiently phosphorylated. Implications for the physiological functions of receptor phosphorylation are discussed.
Schroeder
W
W
Freie Universität Berlin, Institut für Biochemie, FRG.
Meyer
H E
HE
Buchner
K
K
Bayer
H
H
Hucho
F
F
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004789:Enzyme Activation; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation; D010768:Phosphoserine / Q000737:chemistry; D019000:Phosphotyrosine; D011494:Protein Kinases / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000737:chemistry* / Q000378:metabolism; D014101:Torpedo; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry
D010710:Phosphates; D011978:Receptors, Nicotinic; D010768:Phosphoserine; D019000:Phosphotyrosine; D014443:Tyrosine; D011494:Protein Kinases