Effect of glutaraldehyde-fixation on the immunogenicity, particle stability and antigenic reactivity of alfalfa mosaic virus, and the specificity of elicited antibodies.
Glutaraldehyde-fixation was shown to stabilize the structural integrity of alfalfa mosaic virus (AMV) particles as well as to increase their immunogenicity and antigenic reactivity. The antigenic reactivity of the particles was substantially increased irrespective of whether the antibodies were from animals immunized with native or fixed AMV, or preparations of coat protein subunits isolated from the virus. No significant changes in the antigenic specificity of AMV particles were detected following glutaraldehyde-fixation. Thus it is possible to raise antisera to AMV with higher titres by using fixed virus as immunogen.
Hajimorad
M R
MR
Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia.
Francki
R I
RI
D000914:Antibodies, Viral; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000302:isolation & purification*; D002213:Capsid / Q000276:immunology / Q000302:isolation & purification; D000939:Epitopes / Q000302:isolation & purification; D005404:Fixatives; D005976:Glutaral; D000455:Medicago sativa; D009029:Mosaic Viruses / Q000276:immunology* / Q000302:isolation & purification; D014773:Virology / Q000379:methods
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D005404:Fixatives; D005976:Glutaral