Human normal B lymphocytes produce a growth-promoting activity for granulocyte progenitors in diffusion chamber culture after stimulation with pokeweed mitogen.
We have measured the effect of normal B lymphocytes on more primitive granulocyte progenitors (CFU-dG) clonal growth in double-diffusion chamber culture in vivo. It was found that pokeweed mitogen (PWM) stimulated B cells produce a growth-promoting activity which augments the CFU-dG--derived myeloid colony formation in a dose-dependent fashion. All colonies formed under the experimental conditions were composed exclusively of granulocytes at different stages of maturation. Unstimulated B lymphocytes did not effect the CFU-dG clonal proliferation.
Hansz
J
J
Department of Haematology, Academy of Medicine, Poznán, Poland.
Kozłowska-Skrzypczak
M
M
D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D001854:Bone Marrow Cells; D002455:Cell Division; D003470:Culture Media; D015194:Diffusion Chambers, Culture; D006098:Granulocytes / Q000166:cytology*; D006133:Growth Substances / Q000096:biosynthesis / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D008213:Lymphocyte Activation; D011043:Pokeweed Mitogens; D012016:Reference Values
D003470:Culture Media; D006133:Growth Substances; D011043:Pokeweed Mitogens